LOCUS       DADLTY010000001        88440 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.001, whole genome shotgun sequence.
ACCESSION   DADLTY010000001 DADLTY010000000
VERSION     DADLTY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 88440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.001"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(88..1338)
                     /gene="icd"
                     /locus_tag="JN334_000001"
     CDS             complement(88..1338)
                     /gene="icd"
                     /locus_tag="JN334_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAY7436290.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1510..2163
                     /gene="rluE"
                     /locus_tag="JN334_000002"
     CDS             1510..2163
                     /gene="rluE"
                     /locus_tag="JN334_000002"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HAY7436291.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2173..2634
                     /gene="nudJ"
                     /locus_tag="JN334_000003"
     CDS             2173..2634
                     /gene="nudJ"
                     /locus_tag="JN334_000003"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HAY7436292.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2688..3794
                     /gene="mnmA"
                     /locus_tag="JN334_000004"
     CDS             2688..3794
                     /gene="mnmA"
                     /locus_tag="JN334_000004"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAY7436293.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3830..4471
                     /gene="hflD"
                     /locus_tag="JN334_000005"
     CDS             3830..4471
                     /gene="hflD"
                     /locus_tag="JN334_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HAY7436294.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4475..5845
                     /gene="purB"
                     /locus_tag="JN334_000006"
     CDS             4475..5845
                     /gene="purB"
                     /locus_tag="JN334_000006"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAY7436295.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6014..6685
                     /gene="phoP"
                     /locus_tag="JN334_000007"
     CDS             6014..6685
                     /gene="phoP"
                     /locus_tag="JN334_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HAY7436296.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6685..8145
                     /gene="phoQ"
                     /locus_tag="JN334_000008"
     CDS             6685..8145
                     /gene="phoQ"
                     /locus_tag="JN334_000008"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HAY7436297.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPMLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8942..9223)
                     /locus_tag="JN334_000009"
     CDS             complement(8942..9223)
                     /locus_tag="JN334_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436298.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9237..10898)
                     /locus_tag="JN334_000010"
     CDS             complement(9237..10898)
                     /locus_tag="JN334_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY7436299.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELELWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10882..11208)
                     /locus_tag="JN334_000011"
     CDS             complement(10882..11208)
                     /locus_tag="JN334_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY7436300.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11264..11446)
                     /locus_tag="JN334_000012"
     CDS             complement(11264..11446)
                     /locus_tag="JN334_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436301.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11430..11870)
                     /locus_tag="JN334_000013"
     CDS             complement(11430..11870)
                     /locus_tag="JN334_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7436302.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11870..12166)
                     /locus_tag="JN334_000014"
     CDS             complement(11870..12166)
                     /locus_tag="JN334_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY7436303.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12163..12501)
                     /locus_tag="JN334_000015"
     CDS             complement(12163..12501)
                     /locus_tag="JN334_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY7436304.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12498..13709)
                     /locus_tag="JN334_000016"
     CDS             complement(12498..13709)
                     /locus_tag="JN334_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY7436305.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13711..14283)
                     /locus_tag="JN334_000017"
     CDS             complement(13711..14283)
                     /locus_tag="JN334_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAY7436306.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14323..15480)
                     /locus_tag="JN334_000018"
                     /pseudo
     CDS             complement(14323..15480)
                     /locus_tag="JN334_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15781..16083)
                     /locus_tag="JN334_000019"
     CDS             complement(15781..16083)
                     /locus_tag="JN334_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707048.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436307.1"
                     /translation="MEHYWLFVTVRHSYYLKQTDTTEENAMPMKFDEILKQRDKYHAD
                     NMETMNITDYRAFLETGALIEKDHHGFVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16147..16308)
                     /locus_tag="JN334_000020"
     CDS             complement(16147..16308)
                     /locus_tag="JN334_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAY7436308.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(16318..16623)
                     /locus_tag="JN334_000021"
     CDS             complement(16318..16623)
                     /locus_tag="JN334_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436309.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16620..16898)
                     /locus_tag="JN334_000022"
     CDS             complement(16620..16898)
                     /locus_tag="JN334_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436310.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17187..18935)
                     /locus_tag="JN334_000023"
     CDS             complement(17187..18935)
                     /locus_tag="JN334_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY7436311.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18932..19231)
                     /locus_tag="JN334_000024"
     CDS             complement(18932..19231)
                     /locus_tag="JN334_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY7436312.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19237..19470)
                     /locus_tag="JN334_000025"
     CDS             complement(19237..19470)
                     /locus_tag="JN334_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436313.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19472..19692)
                     /locus_tag="JN334_000026"
                     /pseudo
     CDS             complement(19472..19692)
                     /locus_tag="JN334_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19685..19906)
                     /locus_tag="JN334_000027"
     CDS             complement(19685..19906)
                     /locus_tag="JN334_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436314.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19896..20096)
                     /locus_tag="JN334_000028"
     CDS             complement(19896..20096)
                     /locus_tag="JN334_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HAY7436315.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20226..20405
                     /locus_tag="JN334_000029"
     CDS             20226..20405
                     /locus_tag="JN334_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436316.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20530..20859
                     /locus_tag="JN334_000030"
     CDS             20530..20859
                     /locus_tag="JN334_000030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436317.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20909..21097)
                     /locus_tag="JN334_000031"
     CDS             complement(20909..21097)
                     /locus_tag="JN334_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY7436318.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21472..22701)
                     /locus_tag="JN334_000032"
     CDS             complement(21472..22701)
                     /locus_tag="JN334_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7436319.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22950..24071
                     /locus_tag="JN334_000033"
     CDS             22950..24071
                     /locus_tag="JN334_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY7436320.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24120..25346)
                     /gene="pepT"
                     /locus_tag="JN334_000034"
     CDS             complement(24120..25346)
                     /gene="pepT"
                     /locus_tag="JN334_000034"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAY7436321.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25596..26732
                     /gene="potA"
                     /locus_tag="JN334_000035"
     CDS             25596..26732
                     /gene="potA"
                     /locus_tag="JN334_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAY7436322.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26716..27573
                     /gene="potB"
                     /locus_tag="JN334_000036"
     CDS             26716..27573
                     /gene="potB"
                     /locus_tag="JN334_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HAY7436323.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27570..28364
                     /gene="potC"
                     /locus_tag="JN334_000037"
     CDS             27570..28364
                     /gene="potC"
                     /locus_tag="JN334_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HAY7436324.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28361..29407
                     /gene="potD"
                     /locus_tag="JN334_000038"
     CDS             28361..29407
                     /gene="potD"
                     /locus_tag="JN334_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HAY7436325.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29563..30384)
                     /gene="cobB"
                     /locus_tag="JN334_000039"
     CDS             complement(29563..30384)
                     /gene="cobB"
                     /locus_tag="JN334_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAY7436326.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30400..31311)
                     /gene="nagK"
                     /locus_tag="JN334_000040"
     CDS             complement(30400..31311)
                     /gene="nagK"
                     /locus_tag="JN334_000040"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAY7436327.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31340..32584)
                     /gene="lolE"
                     /locus_tag="JN334_000041"
     CDS             complement(31340..32584)
                     /gene="lolE"
                     /locus_tag="JN334_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HAY7436328.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32584..33285)
                     /gene="lolD"
                     /locus_tag="JN334_000042"
     CDS             complement(32584..33285)
                     /gene="lolD"
                     /locus_tag="JN334_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAY7436329.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33278..34477)
                     /gene="lolC"
                     /locus_tag="JN334_000043"
     CDS             complement(33278..34477)
                     /gene="lolC"
                     /locus_tag="JN334_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HAY7436330.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34739..35812
                     /locus_tag="JN334_000044"
     CDS             34739..35812
                     /locus_tag="JN334_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY7436331.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35940..39386
                     /gene="mfd"
                     /locus_tag="JN334_000045"
     CDS             35940..39386
                     /gene="mfd"
                     /locus_tag="JN334_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAY7436332.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39529..40491
                     /gene="ldtC"
                     /locus_tag="JN334_000046"
     CDS             39529..40491
                     /gene="ldtC"
                     /locus_tag="JN334_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HAY7436333.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40574..40786)
                     /locus_tag="JN334_000047"
     CDS             complement(40574..40786)
                     /locus_tag="JN334_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012542232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HAY7436334.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            41071..41703
                     /gene="comR"
                     /locus_tag="JN334_000048"
     CDS             41071..41703
                     /gene="comR"
                     /locus_tag="JN334_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HAY7436335.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41765..42304)
                     /locus_tag="JN334_000049"
     CDS             complement(41765..42304)
                     /locus_tag="JN334_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY7436336.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42531..43835)
                     /locus_tag="JN334_000050"
     CDS             complement(42531..43835)
                     /locus_tag="JN334_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY7436337.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(44243..44785)
                     /locus_tag="JN334_000051"
     CDS             complement(44243..44785)
                     /locus_tag="JN334_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7436338.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44808..45833)
                     /gene="nagZ"
                     /locus_tag="JN334_000052"
     CDS             complement(44808..45833)
                     /gene="nagZ"
                     /locus_tag="JN334_000052"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAY7436339.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45844..46668)
                     /gene="thiK"
                     /locus_tag="JN334_000053"
     CDS             complement(45844..46668)
                     /gene="thiK"
                     /locus_tag="JN334_000053"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HAY7436340.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46649..47290)
                     /gene="lpoB"
                     /locus_tag="JN334_000054"
     CDS             complement(46649..47290)
                     /gene="lpoB"
                     /locus_tag="JN334_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HAY7436341.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(47305..47684)
                     /locus_tag="JN334_000055"
                     /pseudo
     CDS             complement(47305..47684)
                     /locus_tag="JN334_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
     gene            complement(47687..48046)
                     /gene="hinT"
                     /locus_tag="JN334_000056"
     CDS             complement(47687..48046)
                     /gene="hinT"
                     /locus_tag="JN334_000056"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HAY7436342.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48379..50564
                     /gene="fhuE"
                     /locus_tag="JN334_000057"
                     /pseudo
     CDS             48379..50564
                     /gene="fhuE"
                     /locus_tag="JN334_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50624..52057)
                     /gene="ptsG"
                     /locus_tag="JN334_000058"
     CDS             complement(50624..52057)
                     /gene="ptsG"
                     /locus_tag="JN334_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAY7436343.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52352..53149)
                     /locus_tag="JN334_000059"
     CDS             complement(52352..53149)
                     /locus_tag="JN334_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7436344.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(53160..54164)
                     /gene="holB"
                     /locus_tag="JN334_000060"
     CDS             complement(53160..54164)
                     /gene="holB"
                     /locus_tag="JN334_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAY7436345.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(54161..54802)
                     /locus_tag="JN334_000061"
     CDS             complement(54161..54802)
                     /locus_tag="JN334_000061"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAY7436346.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54792..55814)
                     /gene="yceG"
                     /locus_tag="JN334_000062"
     CDS             complement(54792..55814)
                     /gene="yceG"
                     /locus_tag="JN334_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HAY7436347.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55817..56626)
                     /gene="pabC"
                     /locus_tag="JN334_000063"
     CDS             complement(55817..56626)
                     /gene="pabC"
                     /locus_tag="JN334_000063"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAY7436348.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56746..57987)
                     /gene="fabF"
                     /locus_tag="JN334_000064"
     CDS             complement(56746..57987)
                     /gene="fabF"
                     /locus_tag="JN334_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAY7436349.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(58074..58310)
                     /gene="acpP"
                     /locus_tag="JN334_000065"
     CDS             complement(58074..58310)
                     /gene="acpP"
                     /locus_tag="JN334_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7436350.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58521..59255)
                     /gene="fabG"
                     /locus_tag="JN334_000066"
     CDS             complement(58521..59255)
                     /gene="fabG"
                     /locus_tag="JN334_000066"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAY7436351.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(59268..60197)
                     /gene="fabD"
                     /locus_tag="JN334_000067"
     CDS             complement(59268..60197)
                     /gene="fabD"
                     /locus_tag="JN334_000067"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAY7436352.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(60213..61166)
                     /locus_tag="JN334_000068"
     CDS             complement(60213..61166)
                     /locus_tag="JN334_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAY7436353.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(61234..62304)
                     /gene="plsX"
                     /locus_tag="JN334_000069"
     CDS             complement(61234..62304)
                     /gene="plsX"
                     /locus_tag="JN334_000069"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAY7436354.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62385..62558)
                     /gene="rpmF"
                     /locus_tag="JN334_000070"
     CDS             complement(62385..62558)
                     /gene="rpmF"
                     /locus_tag="JN334_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAY7436355.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62610..63131)
                     /gene="yceD"
                     /locus_tag="JN334_000071"
     CDS             complement(62610..63131)
                     /gene="yceD"
                     /locus_tag="JN334_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HAY7436356.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63330..63914
                     /gene="yceF"
                     /locus_tag="JN334_000072"
     CDS             63330..63914
                     /gene="yceF"
                     /locus_tag="JN334_000072"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HAY7436357.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(64026..64985)
                     /gene="rluC"
                     /locus_tag="JN334_000073"
     CDS             complement(64026..64985)
                     /gene="rluC"
                     /locus_tag="JN334_000073"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HAY7436358.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65558..68749
                     /gene="rne"
                     /locus_tag="JN334_000074"
     CDS             65558..68749
                     /gene="rne"
                     /locus_tag="JN334_000074"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HAY7436359.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVDAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68946..69888)
                     /gene="flgL"
                     /locus_tag="JN334_000075"
                     /pseudo
     CDS             complement(68946..69888)
                     /gene="flgL"
                     /locus_tag="JN334_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
     gene            complement(69900..71542)
                     /gene="flgK"
                     /locus_tag="JN334_000076"
                     /pseudo
     CDS             complement(69900..71542)
                     /gene="flgK"
                     /locus_tag="JN334_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71608..72549)
                     /gene="flgJ"
                     /locus_tag="JN334_000077"
     CDS             complement(71608..72549)
                     /gene="flgJ"
                     /locus_tag="JN334_000077"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HAY7436360.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72549..73646)
                     /locus_tag="JN334_000078"
     CDS             complement(72549..73646)
                     /locus_tag="JN334_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAY7436361.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73658..74356)
                     /gene="flgH"
                     /locus_tag="JN334_000079"
     CDS             complement(73658..74356)
                     /gene="flgH"
                     /locus_tag="JN334_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAY7436362.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74409..75191)
                     /gene="flgG"
                     /locus_tag="JN334_000080"
     CDS             complement(74409..75191)
                     /gene="flgG"
                     /locus_tag="JN334_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAY7436363.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(75329..76080)
                     /locus_tag="JN334_000081"
                     /pseudo
     CDS             complement(75329..76080)
                     /locus_tag="JN334_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309482.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(76100..78028)
                     /gene="flgD"
                     /locus_tag="JN334_000082"
                     /pseudo
     CDS             complement(76100..78028)
                     /gene="flgD"
                     /locus_tag="JN334_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(78040..78443)
                     /gene="flgC"
                     /locus_tag="JN334_000083"
                     /pseudo
     CDS             complement(78040..78443)
                     /gene="flgC"
                     /locus_tag="JN334_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78447..78863)
                     /gene="flgB"
                     /locus_tag="JN334_000084"
     CDS             complement(78447..78863)
                     /gene="flgB"
                     /locus_tag="JN334_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HAY7436364.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            79018..79677
                     /gene="flgA"
                     /locus_tag="JN334_000085"
     CDS             79018..79677
                     /gene="flgA"
                     /locus_tag="JN334_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAY7436365.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79753..80046
                     /gene="flgM"
                     /locus_tag="JN334_000086"
     CDS             79753..80046
                     /gene="flgM"
                     /locus_tag="JN334_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HAY7436366.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            80051..80467
                     /gene="flgN"
                     /locus_tag="JN334_000087"
     CDS             80051..80467
                     /gene="flgN"
                     /locus_tag="JN334_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HAY7436367.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80507..82042)
                     /gene="murJ"
                     /locus_tag="JN334_000088"
     CDS             complement(80507..82042)
                     /gene="murJ"
                     /locus_tag="JN334_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAY7436368.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFVLTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(82242..83075)
                     /locus_tag="JN334_000089"
     CDS             complement(82242..83075)
                     /locus_tag="JN334_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY7436369.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(83077..83724)
                     /locus_tag="JN334_000090"
     CDS             complement(83077..83724)
                     /locus_tag="JN334_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HAY7436370.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83735..84319)
                     /gene="rimJ"
                     /locus_tag="JN334_000091"
     CDS             complement(83735..84319)
                     /gene="rimJ"
                     /locus_tag="JN334_000091"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HAY7436371.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84555..85763
                     /gene="mdtH"
                     /locus_tag="JN334_000092"
     CDS             84555..85763
                     /gene="mdtH"
                     /locus_tag="JN334_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HAY7436372.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85827..86474
                     /gene="grxB"
                     /locus_tag="JN334_000093"
     CDS             85827..86474
                     /gene="grxB"
                     /locus_tag="JN334_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAY7436373.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86608..87168
                     /locus_tag="JN334_000094"
     CDS             86608..87168
                     /locus_tag="JN334_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7436374.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            87274..88320
                     /gene="pyrC"
                     /locus_tag="JN334_000095"
     CDS             87274..88320
                     /gene="pyrC"
                     /locus_tag="JN334_000095"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAY7436375.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
BASE COUNT        21155 a        23627 c        21889 g        21769 t
ORIGIN      
        1 tatgctcgat atgctattaa taatatccac ataaataaaa caacgggcgt gttatacgcc
       61 cgtttaaata tttaacacat gtagtgatta catattcttg atgatcgcat caccaaactc
      121 tgaacatttc agcagtttag cgccttccat cagacgctcg aagtcatagg ttacggtctt
      181 ggcgttgatc gcgccttcca tacctttaac aatcaggtct gcggcttcag tccagcccat
      241 atggcgtaac atcatctcag cggagagaat aatagagcca gggtttactt tgtcctgacc
      301 ggcgtatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt caccgatgtt
      361 tgctccaggg gcgataccga taccgccaac ctgcgctgcc agagcgtcag aaatgtagtc
      421 accgttcagg ttcatacagg cgataacatc atattcagcc ggacgcagca ggatctgttg
      481 caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtat tcgggttttt
      541 aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aattcttcac gcgccagctg
      601 gtagccccag tctttaaacg cgccttcggt gaacttcatg atgttgcctt tgtgcaccag
      661 ggtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa ccagacgttt
      721 ggtgccttct tcagaacacg gcttaatacc gataccgcaa tgttccggga agcgaatttt
      781 cttcacgccc atctcttcac gcaggaattt aatcactttc tcggcgtcgg cagagtcagc
      841 tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa ccatatcggt
      901 cagttcaggg tgtttaaccg ggcttggagt gccctgatag taacgtaccg gacgcaggca
      961 gatgtagaga tccagttcct gacgcagggc aacgttgaga gaacgaatac cgccaccaac
     1021 cggagtggtc agcgggcctt taatggcaac gcgatattca cgaatcagat caagggtttc
     1081 agcaggcagc cagacatcct gaccataaac ctgtgtggat ttttcaccgg tgtaaatttc
     1141 catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt cgaccacttt
     1201 cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag ggataatcgg
     1261 attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt gtgccggaac
     1321 aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc gtctgccacc
     1381 acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt tttgttaatg
     1441 atttgtaatt gacttgtcaa tactacccta ttgtttgccg tcatgaaaga tgcgcgttat
     1501 taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac ttcttttaga
     1561 aatcaccagg ttaagcgatt cagctcgcaa cgttctacca ggcgtaaaca tgaaaaccag
     1621 cccacgcgtg tgatcctctt caataaaccc tacgatgttc ttccgcagtt caccgatgaa
     1681 accggacgca aaacattaaa agaattcatc ccggttcagg gcgtttatgc agcaggtcgc
     1741 cttgaccgcg atagcgaagg gttgctggtg ctgaccaata acggcgcgtt gcaggcgcgt
     1801 ttaacccagc cgggtaaacg caccggaaaa atctattatg tgcaggtgga aggtattccc
     1861 acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg ccctaccctg
     1921 cccgcaggcg cggagctggt tgacgaaccc gcgtggttat ggccgcggaa tccaccaatt
     1981 cgtgaacgca aaagtattcc caccagctgg ctgaagatca ccttatatga aggacgtaat
     2041 cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact gattcgctat
     2101 gcgatgggag attactcttt ggataatctt gccaatggcg aatggcgaga agtgacagat
     2161 taaggatgca aaatgtttaa accgcacgtt accgttgctt gcgtggtgca cgcagaaggc
     2221 aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca acctgccggg
     2281 catctggaag ccgatgaaac cttagtggaa gccgccgccc gtgaactgtg ggaagaaacc
     2341 ggcatcagcg cgcagccgca acactttatt cgtatgcatc agtggattgc gccagataaa
     2401 acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc gacccagcct
     2461 catgacagcg atatcgactg ctgccgttgg gtcagcgccg aagaaatttt acaggcgtca
     2521 aatcttcgtt cgccgctggt ggcggaaagt attcgttgtt atcaaagcgg gcaacgttat
     2581 ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat ctaaaaggct
     2641 gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg tctgaaaccg
     2701 caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct gcctggctgt
     2761 tgcaacaaca gggatatcag gtcgaaggcc tgtttatgaa gaactgggaa gaagacgacg
     2821 gtgaggaata ttgcacagca gcagcggatc tggctgatgc ccaggcggtc tgcgacaagc
     2881 tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaac gtcttcgaac
     2941 tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg tgcaacaagg
     3001 agatcaaatt taaagccttc ctcgaatttg ccgccgaaga tttaggtgcc gattatatcg
     3061 ctaccggtca ttacgtacgt cgggccgatg tcgatggcaa gagccgcctg ctgcgtggtc
     3121 tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag cagattgcgc
     3181 aaagcctgtt cccggtaggc gaactggaaa aaccgcaggt gcgtaagatt gctgaagatc
     3241 ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt ggcgaacgta
     3301 aattccgcga gttcctgggc cgttatctcc cggcgcaacc gggcaaaatc attaccgtcg
     3361 atggcgatga aattggtgag caccaggggc tgatgtatca cactctcggt cagcgtaaag
     3421 gtctgggtat cggtggcacc aaagaaggta ccgaagaacc gtggtatgtg gtggacaaag
     3481 acgtcgaaaa caacattctg gttgtcgctc agggccatga acacccgcgg ctgatgtctg
     3541 tcgggttgat tgcccagcag ctgcactggg tcgatcgcga accattcacc ggcactatgc
     3601 gttgcacggt aaaaacccgc tatcgccaga ccgacattcc gtgcaccgtc aaggcgctgg
     3661 acgacgatcg cattaaagtg attttcgatg agccggttgc cgccgtgacg ccgggccagt
     3721 ctgccgtctt ctataacggt gaagtgtgcc tcggtggcgg tattattgag cagcgtctgc
     3781 cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg tggcaaagaa
     3841 ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc tggtgcaaca
     3901 actcgctcac caggggcatt gtgatgccga tgcgctacac gtctcactca acagtattat
     3961 tgatatgaac cccagctcga cgctggcggt ttttggcggt agcgaagcca acctgcgcgt
     4021 cgggctggaa accctgctcg gcgtgctcaa tgccagcagt tgccagggct taaacgccga
     4081 attaacccgc tacacactca gcttgatggt gcttgagcgc aaactctcct cagcgaaagg
     4141 cgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg aacacttcga
     4201 tttacagtcc gaaacgctga tgagcgcgat ggctgctatc tatgttgatg tgatcagccc
     4261 gcttggcccg cgcattcagg tcaccggttc ccctgctgta ctgcaaagcc cacaagtgca
     4321 ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct ggcaccaggt
     4381 cggcggcgga cgtctacaac tgatgttttc tcgtaatcgc ctgaccactc aggcaaaaca
     4441 aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac tgaccgccgt
     4501 ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga ttttcagcga
     4561 atatggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa aactggccgc
     4621 gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt accttgatgc
     4681 aattgtcgcc aatttcagcg aagaagatgc cgcacgcatc aaaaccatcg agcgtaccac
     4741 taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg agatcccgga
     4801 actgcacgcg gtttctgaat tcatccactt tgcctgtact tcggaagata tcaataacct
     4861 ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt actggcgtca
     4921 actgattgat ggcattaaag atctcgccgt tcagtaccgc gatatcccgc tgctgtctcg
     4981 tacccacggt cagccagcca cgccgtcaac catcggtaaa gagatggcaa acgtcgccta
     5041 ccgtatggag cgccagcacc gccagcttaa ccaggtggag atcctcggca aaatcaacgg
     5101 cgcggtcggt aactataacg cccacatcgc cgcttacccg gaagttgact ggcatcagtt
     5161 cagcgaagag ttcgtcacct cgctgggtat tcaatggaac ccgtacacta cccagattga
     5221 accgcacgac tacattgccg aactgtttga ttgcgttgcg cgcttcaaca ccattctgat
     5281 cgactttgac cgtgacgtct ggggttatat cgcccttaac cacttcaaac agaaaaccat
     5341 tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgatcg acttcgaaaa
     5401 ctccgaaggg aatctgggcc tttccaacgc ggtattgcag catctggcaa gcaaactgcc
     5461 ggtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc tcggcgtggg
     5521 tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca aactggaagt
     5581 gaaccgtgac catctgctgg atgagctgga tcacaactgg gaagtgctgg cagaaccaat
     5641 ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga aagagcttac
     5701 tcgcggtaag cgcgttgacg ccgaaggcat gaagcagttt atcgatggtc tggcgttgcc
     5761 agaagaagag aaagcccgcc tgaaagcgat gacgccggct aactatattg gtcgagctat
     5821 cacgatggtt gatgagctga aataagcctc aaatcagtgc cggatggcga tgctgtccgg
     5881 cctgcttatt aagattatcc actttttctt ttttcacttt acctcccctc cccgctggtt
     5941 tatttaatgt ttacccccat aaacacataa tcgcgttaca ctattttaat aattaagaca
     6001 gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct
     6061 taaagttcag attcaggatg ctggtcatca ggtcgatgac gcagaagatg ccaaagaagc
     6121 cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgcctga
     6181 cgaggacggt ctgtcactga ttcgccgctg gcgtagcaac gatgtttcac tgccgattct
     6241 ggtattaacc gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ccggtgctga
     6301 tgattacgtg actaaaccgt ttcatattga agaggtgatg gcgcgaatgc aggcattaat
     6361 gcggcgtaat agcggtctgg cttcacaggt catttcgctc cccccgtttc aggttgatct
     6421 ctctcgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac
     6481 cattatggaa acgttgatac gcaataatgg caaagtggtc agcaaagatt cgttaatgct
     6541 acaactctat ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg
     6601 tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtc attaccaccg ttcgcggcca
     6661 gggctacctg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctcg
     6721 ctgcgggtac gttttctgtt ggcaacggca gcggtagtac tggtgctttc gcttgcctac
     6781 ggaatggtcg cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta
     6841 cgtggcgaga gcaatctgtt ctataccctt gcgcagtggg aaaacaataa gttgcatgtc
     6901 gagttacccg aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac
     6961 gggcagcttt tatgggcgca acgtgacgtg ccctggctga tgaaaatgat ccagcctgac
     7021 tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg
     7081 ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac
     7141 gcggagatga cccactcggt ggcggtaaac gtctacccgg caacatcgcg gatgccaaaa
     7201 ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg
     7261 agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc
     7321 gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa
     7381 gaacataacc gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga
     7441 aacctgaacc gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc
     7501 accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct
     7561 ctgcgtagtg aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc
     7621 cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg
     7681 ctcagccgcg agctgcatcc ggtcgcccca atgctggaca atctcacctc agcgctgaac
     7741 aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt
     7801 gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa
     7861 tattgcctcg agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg
     7921 gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt
     7981 caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc
     8041 accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg
     8101 gaggtgattt ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccttttacc
     8161 actacatcaa gtcattgatt cgatcaaaca attttcattg aaaaagtgcc aaaaatcgca
     8221 aattgactac accattaact acaccgttcg gtgcactgta tgaaacaacg tggaacaaat
     8281 agacacaaga aatatacagg cgggtaatct ttccaggggg aagcgcacca attcatgagg
     8341 ggcgttaatg tcgatatggg gatccccata acggggctac cggatttttt ttccggttaa
     8401 cctttaatca ggctggtggg ttttacatac ccttgatcac cgtaatgatg atccagatgt
     8461 gcaccttttc caccaatggc ggaaaagctc caaccgtaac gatgatcgct atggttgcct
     8521 cgatggtctg cgtggtcatt atgaccgttc agccagtgac cttaatcaga atttttcgct
     8581 aaaaaatgac gttggccacg tcatccggaa agcacaaaat cacgtcgatt tttaatgaac
     8641 ggttaaaccg tctttttgtc acggtgtgtc actggttgtc actctccgtc acgattgagg
     8701 tggtggtcat tctgcccgac cgcgcatctt tgcgctatcg ggaatatcaa acagttacct
     8761 cgccgacttc ctcaaattga ggtttccgaa aatatcagag cgggttagcg attaagctga
     8821 cgagtaatta aactgcgaaa atttcgtagt ttctgaatat attccggcct gctggttaac
     8881 agtcctcaga tgtgagggat gtagccagcg caattttgcg ctttgcttta atctcagtga
     8941 gttaccgccg taaccgctcc ggcttcttcc agtggtacgt gattttctcc ttctcccgat
     9001 acagttcaac gcggcgattg taggccagca tttccagaac gcggatccgt atgtcgcgca
     9061 tatccgcatc attaagctgg ataccatcac ggcgcatcac ctcagcaaca acacgcgcat
     9121 aattttcggc tgtcacgctg tccggctgcg tggtctgttc gtcagcctgc tggctgattc
     9181 cagagacgcg gcggattaat cgcagtattt cggcttctgt catgctgctg acctcattac
     9241 accccgctaa attcttccag tttctggcgg tggctgtcgc ttatatcaaa agcaaaatcc
     9301 tcatgctctg cctggaatgt accaaacgcc atcagcgccg ctacgctcgg gtctatcttg
     9361 ttcggtgatt ttttcttgtt cggcttgata ttggcgttcg cgtcactctg catcacgacg
     9421 ttactcatcg accaggacaa caccggatcg ccacgatgca caatcaccct gcggttaaca
     9481 aaaacttcga acgatttcgc cgccggactg aatctgaggt aggtttgcgg gaacggctcc
     9541 acctcaaaac cagccccctg taattgcgtc ctgagatgcg tggcgttcca cgtatcaaag
     9601 cccaccagcc tgatattaaa tttctccgca tcctgcatga tgtcatctct gatccggtcg
     9661 taatcaatgc agtcacctgg cgttgtgcgt atccagcccg ctttagccca ctggcgatag
     9721 acagcgcggt ttttattggc ggggttctgt agctggaact ccggcagata atgacgggaa
     9781 atcagcataa tggttttacc gaccggaaag gcatagcata cgctggaaat atcgctggtt
     9841 gatgataagt ccagccccgc gtaacactcc tgaccgtgta aatcttcctc cgtgaacgtt
     9901 ccggcacact cagcccatgc accattaccc atccacgggg tagtcccctg acaccagata
     9961 ttaaatcgct ttgtcatcat ctccacccac tgcgacggaa taccccgcgc tttctggatg
    10021 gttgaggcca gtttttcacg atccacggaa acatcaatat tagggttcgc ctttatccac
    10081 atcgccggat catcaacctc gttttcgtcg tccagttcgt aaatcagtac aaaaattgaa
    10141 tcgttaacct cttcgccgtc caggatctgg cagcaataat catagtgctg tttacaggct
    10201 gaaacgacgt tactgcccga tgtggtaatg gcaaataaca gcccttcggg acgtgcgccc
    10261 atccccagct caagcgcgga ataaacgccg ttatcggggt gtaggtggta ttcgtccacg
    10321 atggaaagac tggggtttgt cccctcgatg gttgccgctt ttgctgccag cggctttaac
    10381 aggctgttgc ttttcgggtg tatcacctta tgcgcctgaa tattcacccg cctgcgtaac
    10441 ggtcgggata aaaggcacat ctgacgcgca ttatcaaaca cgatccgcgc ctggtcacgg
    10501 ctcaccgctg cggtgtagat atcctgctga ccgttctcca taatcagaaa ccagttagcc
    10561 agaatcgcgg cggtcgtgga tttcgcattt ttgcgcggca cttcgataaa ggcgctcgtg
    10621 tatttgcgcc gtccggtggc cttaacctta aagccgagga tgcacgcaaa ggcgaactgc
    10681 tgccacagct ccagctcaat gggtctgcca cgcatcggtc cttttacgtg cgggcacacc
    10741 ctggaaaagg caataaaccg ctccacaacc tcacgatcga acgtgtaaag ggggctttta
    10801 aggtccgaaa agtaccgttt aacagcctgt tttaaccgtt tacaggccgg aattttgccc
    10861 gtttttacgt cttctgcgta cttattccag gcggtcaagc tcgtcctctt cttctgtttc
    10921 cggtggattt ttacggcggc ttatcgggtc aaaaccgagc aaggaggcca tttttatcat
    10981 cactctttca gcgtcggatt ttgcgcttaa tgcggggttt ctgctctcgc cgccctgact
    11041 gttaacaatg ctgaacccgc gcgccgcaag gtctgcaacg gctttccggt aaatggagta
    11101 gttgacgcaa tacagttcca gattgctcca gtcggcgggg gtcaggtctc cccgttccgc
    11161 aagctgccgc gatttttccc gccactgctt cacggcgata tcatccaggt aggcggggtc
    11221 gggtttgtcc tgaaaacgcc ccccaccccg tcatacagcc tcatcagcga ttgcggaaac
    11281 attccataac ctcgcggtca cggtcggtta atcgcttcgc tgtgatgcgt tctgcgcgtc
    11341 ctgacgcttt atctttatgc cctgtttcct gtgtcttcca tgcgtcacgc tgccttataa
    11401 gtccacggat aaggcggttt tgttcccgct cattcatcag cgtcatacat ccagttattg
    11461 cggttagcag cccgttcttc ctcttcacga aatccacctg cggcacgctt gctttttgtt
    11521 gccggatcaa gccatttcgt tttctggtta tgacacgcct ggcacaatgg ttgatggttc
    11581 cattcgggcc agaagagaac atcatcaccg ccattaatcg ggataatgtg atccaccacc
    11641 acggcgggcg tatatatccc cttctcaagg catcgcacgc ataacgggtt tttactcaga
    11701 tacatggcgc ggtatttgtc ccactgtctg gaatatccac gcgcgcggcg gtgtcctcgt
    11761 ctggcatcct ctgcacgcca tgccgcccgc ctgtgctcct cacacttgcc ggatttaacg
    11821 cgcctgttac agcctggttc tgtgcatcgt cttaatggtt gccacggcat cagtaccccc
    11881 ccacatcacg gtaagccgtc cagagtgcgc ctatcgtcat gggtacgcgt gtttttgcgt
    11941 tatccgcgac aatctggcga ttttcataca ggtgagcgat aaacatcatg cagccaatct
    12001 ttatggctgg cgtgaacgcc agcccgtcat caaagcgcct gcctatgtgt atctggcacg
    12061 cctcaagcga tgcggcaatg tatccgctga ttaactggtc ttcatcgtcg ccatcgatgc
    12121 ggcaatggag tttcacttct tccagggtga taagttcttc tgtcattttt ccgcgccctc
    12181 acgacaaaga atttcaaggc gtgtcctggc ggcatccggc agcggctgcc cgatgatatt
    12241 cagtacacgc cccgccagcg gccccgtact gacctttatc cggctggtgg cgttgatgtc
    12301 cttccggtac cgtatccaga tccttacggt accaatcgcc agctctgcgc ccgatgaaat
    12361 ggcctccttg ctgctgacca tgttcacgct tgcccagagt gtgtgaccgt cctcccacgt
    12421 ttcgagtatt tcgccggtta tatccctggt ctgtttcagg gtctgaatcg ttatcctgtc
    12481 acgcagtcgc cctatgttca ttccggtttt tctccctcgc tgatttttac ttcctgtttc
    12541 catgcctggc tgaactcatc accaccatca cgcggtgaca gcccttcacg ttcgcgggct
    12601 tcgttcggac acataacgtc ggattttatg ccgcgctcat aggtggcaaa gcgttcgcca
    12661 ggtgtggccc gtaacaggtc cgcgctgtca aactccacct gataccggat accaggtaca
    12721 ggcgaggcca ccagcaaggc gttttttatc tgctgctcaa agttcgccag ccacgggcgc
    12781 atcgtcatgg tgagaaatgc gcggcttgcc tcgctgaagt tgctgtaggt gctgttgctg
    12841 tattcctgca aaaaaatcgg cgagacgttg aacattcggg cgatgtcttc aatggtgaag
    12901 cgacgggagg ccagccattc ggcatcctga ttactcatgc ccagctgctg atagctcatc
    12961 cccccttcaa ggatgggcgt ttttccggcg tttctggccc ctttgtagcg ttccagtgcg
    13021 gctaatgcct gtttgccctt cacgccgtcc agccattcgc ctgacgtgat aacccctgcc
    13081 gccatcatgc catcacgcat cacgctcgcg ccgtggcgtt gttgggccag cccaagcccc
    13141 agcgattcgc ggcagatggt tacaggtgag cgccccataa aaccgtcatc cgtggagtaa
    13201 cgaaggtgga gaacctccca cggtaaatag ttgcggatgt ttccggtgta ggcgtcagtg
    13261 atgcagtagc gccagttatg atcacctgtc tgctccacgt tcaccgactg cggcgggtaa
    13321 ggatgtaaag ccgccggaaa accatcacgc ccccactgaa taaccgcata agcattacca
    13381 cttaacaggc agtagcggat catcattcgc ttaaactggt agggtgtctg ccacgcgttc
    13441 gggcgctcgt tgaggatgtg atcgaccgga tgagaatcaa gccattcgcg ggcctccttc
    13501 cccttctcat tgcgtaccag gtacaggtaa cacggcatgg tagccaccgc gtcagagatg
    13561 accgtgacgg cgttcatgac ggcggggagt gattccgctg tcccagatga tacgtactca
    13621 cccgcccccg tgttcgatgt gccggccagc gccataaatt catcaagcgt catgctgcgc
    13681 tgctcttttt ttcgtctgaa aggccacata tcacaccccc gctaaatccg cccaccagcg
    13741 gcgattatcc gcacgcggca ttttttcggg gtgctgctca tacagggaac ggcgggccag
    13801 ctccacgccg gaatcggggt aagccggtac cgatgtaacg gtaatttcgt acagttccgc
    13861 caccagcacg gtgcgcacgc atggatctgt tgtggtatcc catacatcct tacgggagcg
    13921 aaagccaaag ctcatccccg agatgtcccc acgcttaacc agttcgataa cgtcgcgccc
    13981 tgtactggta tccggtgggg tcagttcaaa gcgtaaccct gtttcgtcct cttccagttt
    14041 cagcgtgccg gaacgggtgc gccccagtaa catgctgtgg tcatgctcat acaggccgcg
    14101 aacgtcatta cccgccgcaa gccattcagt aaacgccccc cgctggaatt tttcgtaaaa
    14161 ctcaccccat aacagttctg aaagcttatc ccagcgaaca acgtagcccg tcagcgtacc
    14221 ggctccgctg gtggtgattt ccgatgaccg gatttccata ctcttcataa ttttttcacc
    14281 cataaaacac taaaggggct ttttagcccc ttctgtatgc tgttaatcgt cgtcttccgg
    14341 cagtgccaga atcttgatcg cgttcgaatc caccacgcca ccgcctaaat atttttgcgt
    14401 gaaaattttg atgaatcctg gttctgtcag gttgtccggt ctggtgcgaa cacctgtttc
    14461 gtgatcaaca atgtagtaac cgcgtttgaa gtcacccagg gcaataacgt tatcaggcat
    14521 aaactccaga tattcgaccg gaaggcccag caacgtatca ggatcacccg cctgtaaacg
    14581 gtcgcgccag atataatcac cgttcgcgtt cttcaccttc tgaagttttg ccgccgtcgt
    14641 ggagttaacc actcagaccg cgtttttgcg gtatttttta cgtaatgcaa atttcaggtc
    14701 gatcagcggg tccgcagatg tccacgccag agattcggaa ggtttaatta cctgcaacgt
    14761 accaaaatca cgctctttgt cgttcttctc tgcacggggt acggataaaa aaccttttgc
    14821 ttttttgtca ccgtcgccca caaccagatc gctttcttcg gtttccgtga aggtgtcgcc
    14881 aatctcaccc gtcagccatg aaaggatgtc cacatcggaa aaatccacga tttcctgtgt
    14941 ggtgcgcggg tacgcataga ccggatacag cttaatgctc acctcgttaa tctgcggggt
    15001 gctggtctgt tcgcgtgcct taccctcttc accgtggtta acggtcgcac cgccagcgga
    15061 aacaagctgc ttaaactcgt tgctgctgat tttcttcacg gtacagatgc gacgcatggt
    15121 ggattcatcc gccagcattc gcatgatttc ggtgttcagt tcagggataa cggtataacc
    15181 accatcagcg ggaacgcctg tacttaatgc gcgggtttca ccggtcagaa tgtagttacg
    15241 tagttctgcg gggtcagtgg tctggctgct tttacctggc ttgctgcgct cttcgtctgc
    15301 aatggcttca aggcgggaaa tgtctttatc gagggattca gctttagcgc gtaattcgtc
    15361 aaattttgcg ccctcagcat cgttaagact gcggttttct ttttcagcgt tctccagcat
    15421 gtcgcgcatc tgatttttaa tggcggtttt ctgctggcgt aattcgatta ttctctgcat
    15481 aaaaaaagtc ctggggttaa gtaaggaact ccaggacgcg gcaaacactc aaccgttttt
    15541 cataaggaaa tcagtaatcg caccgatcgt tttcccgcct ggtaatgaat attggcgagc
    15601 acattaacag gcgggaaagt ggccccagcg tcctggcacc acgggcgaga ataatcatga
    15661 ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa cgtggaacaa ccacgaacaa
    15721 ataatttaca aaaaataaca aaaagccgga ttgctccggc tgtatgtgct gtatgggtgc
    15781 tcagtctctg atccctttca gaaattctat caatgcatct gtctgttcag gatttactgc
    15841 cagcatttca ccggatagcg cgcaacgaac aaaaccatga tgatcctttt caatcagcgc
    15901 ccccgtttcc agaaatgcgc ggtaatcagt gatattcatc gtctccatgt tgtcagcatg
    15961 gtatttatca cgctgtttaa gtatctcatc aaatttcatc ggcattgcgt tttcctctgt
    16021 tgtgtctgtt tgtttcagat agtaactatg ccggaccgtg acgaaaagcc agtagtgctc
    16081 cataccgttt caaatgacgc aaaaaaagcc ggattgctcc ggctgcgtgg tgtggttctg
    16141 atattcctac tctgctaatt tgctcagccc catgtctgcg taagtgcgat ttactgcatt
    16201 tctcaggtct gcgtagttct ccgttggcgg ctccggtgtg cccttgctgc tctgttttac
    16261 cagcgggtcg cccaccagct gcccgtaagc tgcaatttgt gctgtcatat tgccacctca
    16321 gtgaagcgat ccggcattgt tctttttcat ctcttccatc attttttcag tgagcattac
    16381 gcactgaaac cccatgtttt tgatgtctac gggtttcatg cctttgtcag tggatatcac
    16441 aggcagaata tcctgataca catcgcggtc agggtaacta agcacacaaa gctcccatga
    16501 tgccacgggt gaggatcctt cgtctgtttc aacccaccag cctggtgtag ctgcgcttat
    16561 ttgcagaatc gtgatccgtc cgttgtgctt gtaaaatttt tcaccaaatt ttggttcgct
    16621 catgattcca cctctccggt tttaacgttg attgttgtta cctgttccgc ttcggcaatc
    16681 tcccgctctg tcagcgtggc aaaatttgcc gccgtcgtgg tcatgaatgc gcttatcagt
    16741 tcgggatgtg ctttcgcgta tccttccccc gcgtggcggt ctatcgttct gattgccacc
    16801 tttaaagcgt gctctgtcat gtctaacgcg cgatatttcg gttctgttct gtctctgcgt
    16861 tttttgaatg atttattaaa tttccctgaa gtgtgcatat ttatttttac cccctcgttt
    16921 aaaaagtttt gagttgtgcc tccccttgtc taccttatct accttagtgg ccctcatgcc
    16981 agtaatggcg ctgctttcag cggggtagag tgcttttatc cactatctac cccgtgtcta
    17041 cctccctgtc tgattcaggt aaaatcaggt agagagggta gatagtgggt agacagtaaa
    17101 aaaaggctat ctacctaact taatgcactg aattaaatgt atttttcttt actcaggtag
    17161 acagggtaga cagcaattac aaaaaattat aaaaacgcgt cgcactcgtc tgttgttatt
    17221 gcgttagtct gcgttactcc cttaactttc cgcgtaatat attcatgtcc gtaaactttc
    17281 gcggctggct tcatagcctt gccaaagtca tttacgttta gcggtttgct cctgcctgcg
    17341 tacgccataa acgccagata gacgcggtaa aggctgtttc tggtcgtgta cttcactgaa
    17401 tcgccaccgc cgcccatcat caggccgcgt gcttcctcca gaaaattcag gaactggcaa
    17461 aactcaataa ccggatccgt ctgttgcttt attgccagag cttcatcacc gtcacgctgt
    17521 tccagtagta aagcccgtgc tttctcagag tcggtaaagt tcgccagcaa tcggcggata
    17581 atgacaggga tttcagccgc aatcttttcc ggtagctccc tgtctttttc ggcctcacta
    17641 acgatattgt cgaaacggaa aatcacgcga cgacgtgcca cacctccggc ccgttcggtg
    17701 aatatcatcg ggttattgtt ggtcgccagc accaccgccc tgattacagc cgtgaaacgc
    17761 ttttcgtatt tcgggttaat ttccacgggg tcaccgcccg tgattttctt gatgcccgtt
    17821 ccttcgcctg tatatttcgg ctggtcagcc aggacgataa gacgactccc gacaacctgc
    17881 gcacgtccac cagcatcatc aagcgatgtc atttcagcgc ttaccgtgtt ctgtttccct
    17941 gccagaaggc tggctatgtg tgtgaatgta cttttaccgc tcccgccgtc tccggtggcc
    18001 tcaataaaca tctgccagtc gtaccggttc gccataatca tgtacagcgc ggcacatata
    18061 cgcatcatct tgcgctggtc ttttccggct gcgtgctcaa gccatttatg aaagtttagc
    18121 gcgttatcgc ggatgttctc ccctggtgct ggtggcgtgt actcaatgcc gttgtgcgtg
    18181 gtgatccagt tctccggcgt gtgcggggaa aattcccccg ttttcaggtc aagcgcacca
    18241 ttggcgaacg gcagcaaatc gccggacggc tcgcccattg gttcggcaat aacttttaac
    18301 gcttccacgg cgttattgat tacgcgcttg ctgaaagtgg ccctgtgctc tgaatagatc
    18361 accaccattt cgcggctaag ttccattgtg ctgaccggac accatacccc gccgcgccat
    18421 acgtgaacga tttcactttc ctgatgcaca caaacgccat caaagcgctc ggcaagcagc
    18481 tgcgcgcgct cactgtctgc catttgtgcc agttgcgtat ttttctgctc cggcatggta
    18541 agccctgcgg caatattttc acgctcagca ttcagatagc gccgccagtt ttcacacttc
    18601 tggacgtgca ttccttcggg gtaaaaattt gcgtcctgaa tatctgccgc cgccagcttc
    18661 tgaccaatct ttttggtctc caccaaatcc agttctccgg cctggtacag ccttacgcgt
    18721 ttttttccat ccggaacaat tttcagtgca tcaagttcgg caagctgatt tggcccaagc
    18781 cacacaggcg gcacattatc gccggatgcg gggccatcct gctcctgcca ttgtttcgcg
    18841 tgcgaccagg catcactacc cgcaaagata attacctctg tgtctttgtg ttttattccg
    18901 cgtggctgtt tttttacgtt cggtgccagt ttcatttttt acccctgaat acgttaagca
    18961 tcttttttat ttcctgaata ttggcgcgtg ctttctccct gctggttggt ttactgcggg
    19021 gcgctgcctg taccagcgtg aaatcacgcc ggaattgata aacaggcatc acgcaatcat
    19081 attcgtaacc ttcacggcgg taggtgatgc gccgttctgc cacgccttta atcgttacca
    19141 tgccgccgta ctggtcgcgg taaatatcac cgcgcgtaaa tttagggtga gtgttgccac
    19201 tggcagttaa gccagaatat ttaagtttca ttatttttat tctccggtgt gttgttcttt
    19261 atatctgtcg tgcaatagat ctatttcttg cagttccatt attacaggct caagaagcgt
    19321 tattaatgcc gtggcaattc ttgatttttg tttgtcgcgt tcattgtcgc caagtgtttc
    19381 aagccatatg cgcaatattt ccagcatgtt ttcactgtga gaaagtgcaa gaaatgcgcg
    19441 gtctattgtt tcgtggtaaa tatcacgcat attaatcccc gtccgtcgtt tttcttaaaa
    19501 taacctctgt cacgaaatca gcataatcgg cggcgatatt cagtacatca agcccgttga
    19561 tttatattct ctcgtggaaa gtaagaaaaa agccgctcta ataagttctg gcattgacga
    19621 aagcgcatca gccgcatcat caggaacgcc ggaaaattcc tgtttcaggg aattaaaacg
    19681 atcatcacgc ataacccccc ccattttcac aatcggcaac aagaatattt ttagcgtcat
    19741 tcagtgaccg cgttgcgatg tatcggatac attccagggc gaactgtgtc cggtctttct
    19801 gttcttctcc ctgcgcaagc tctgccgtgc attcaatatc aataagcgcg tgcatcagcg
    19861 tagtgagtgc ggcggctgct gcgttcggtg tggttttatt gtgcataggt ttccccctga
    19921 cgaatacggg cggcgaatac agcaacacaa ccggacgggc aacggctacg cgcttcgcgt
    19981 tccgtccaag cggttacgtg gatgatttgg gattctccgg cactcagtgc cagaaaacgc
    20041 cacacaaagg ccgtttgtgt gtgtacaagg tgtggtatat gatttacggc aaccataacg
    20101 gctcctagtt tacgttgttg gttagaagcc ccgttactgc tcctacagtg cggggctttg
    20161 ctttttgtgt tgattgtgtg taatgtgtca ctacacataa atacattacc tgaggtgtaa
    20221 ttaacgtgtc aacacacaaa aacgaaaggc gtggtaatcc tccgttccag ttccgtttag
    20281 atcctgactt acgggaaaag atggaaaagg cacaacaaca ggacggcgat gattctcttg
    20341 ctgcctggat aaagcgaata atccgcaaag aattacagca acgcggcata gaacccaaag
    20401 gataaaaact atcagcgccg tggtgtgagg tactacggcg cattgcttta caggcgcaca
    20461 caatgaccaa cgaagaatca accgaaaaaa catcacctgc acgaaaaaga cgacgcagaa
    20521 agaaagagca tgaatcagaa cgattcgcgc cgtgttcttt tgcccttgag aagtacctta
    20581 aagaatactc atcaaaggag agaacttcgc agttatggca acgcactgaa ccagactgat
    20641 tgcattgccc accagcctga tagcggctat catccccgtg cttatgtttg ggatcacaca
    20701 cacaaacggc gcagcgggtt atctgttcag aaaggcggct ccatttcggg gccgcttttt
    20761 ttatgcctga aaaaccccaa ttttgtggtt ttccagtttc accagggcga acgaatcccc
    20821 gcccacgttc tggcgtatat tcaatcttca tggttatagc tctgtgttca gatgattgat
    20881 gtgtggcggc tgcgtgccgc cagcgtgatt aatgaactgc cttgcagcta tccttccagg
    20941 ccagaacctc ggataaagac cagccaacgg aacgaccgcc aagtttacga cgtgatggga
    21001 attgtccggc cttttccagg cggtagcggc atgagcggct aaggcctgtt agcttttcgc
    21061 attctttttc acgtataaac cgatcagtgc ttaacactat tgcccccttt cgtttcttaa
    21121 agagttattt cgtgttctat tgcgttggga tgtgtctgat tgtgtcagga tgattcagag
    21181 ttggcaaatg ttgaggccgt atggtttaca gaaagaggaa taatcaggat aaaatccttt
    21241 aaattcatgt taatacaaag gcataaaata ttgtttcatg ccttttttct cgctatttaa
    21301 agagtgattc gctagtgtat aaaaaatcag taacacatta aaaatcagct acttataaat
    21361 ctgtacactt tttcacctct tgttcgtagt tgttccgcat tgttgctcat tgttgcacgt
    21421 tgtatctgtt cgcatatcca gtatgcgcat actgaaaaaa cacgaaaaaa attattttct
    21481 tctggctact ggtagcgtgg ttacgttttc atgtgttccc gccagtatcc ctaaccgctc
    21541 cgtccacata tccagcgcat tgcgtttagc atccagataa cgggaatgat tataaactcg
    21601 ctgcattcct ggcatctggt ggcctgtaag ctgctccacg acgtgaggat ccacgcctaa
    21661 atcgttcagc atcgttgtaa aggtgcgccg gatgtcatgt agtgaccagt gagggtgatt
    21721 tagcctcctg tgcgctaatc tgccgtactg cgacacgctt gtttcctgtt tcacttcccc
    21781 cagcaataag cccgtgtgcc tgttctgctc caccagctgc gtgacgaacg gcagtattgc
    21841 ttccggtatg ggccggaata ttgccacctt cgttttgctg tgttctttcg gcacggtcca
    21901 gagcatttcg gtaaaatccc actcgctgat ctccgataac ctcagttcta ccgtccggca
    21961 tccgaacaca atcaggaggc ggattaacgc gatgtagtag ggggagaata tttttttgtc
    22021 cagtgcctgc aataattcgc ccagttcttt ggtgcttaag acacgctcgc ttatatccgg
    22081 ttttttccca acgtccgcca cactcatatc atcaagaacg ttgctgattg catagcgccg
    22141 cctccggcag aacttaagcg cctgtttgca cgtctgtagc aagaatccgg cagtaacagg
    22201 cgttcgcttt gccacctggt caaaacaggc cagccagtgc cgtagctcgc atttatccag
    22261 cggcatagca ccaatgtgct gtattacgtg attattaagg cgctttttca gggcggcata
    22321 atccacgcgg ttttcctttg cgtacgactc aagccagtag gtgagcgcat cgccaaccgt
    22381 taccggcttt aacgcttcct gtacggtgta attaagctca tgacgtggat ttttcccctc
    22441 tgccagccat gcgcgacact gggcggcttt ttccctggct gatttcaggc tcagatcagg
    22501 ataatttccc agcttaatgc gttccggtgg tgcccccctt cccgttccgg ccctgtaagt
    22561 gaaataccag gttaaaaggc cactggttga atgcctgacg ctcaggtttc caccgtcatt
    22621 aagaaaggct gttttttggg cgggtgtgcc gttgattttc ctcagctgtg tatcgctcag
    22681 tttgttaagt gctctgctca taattttgat tccggtcatc acaattaact acaccattaa
    22741 ctacaccgat cgttgcacaa agggctacaa cgtgaaacaa gctggaacaa ggaaaatcac
    22801 aaatctgttt ataatcaaaa acataatgaa caatctgaaa cattatgaaa cggcaaaaaa
    22861 cactaattga taaaatatgt ccatacttca cgcattacgt taagcatccg ttataatcgg
    22921 ttgcagatac cagcctgtgg atgcttaaca tggaatacca actcactctt aactggcccg
    22981 attttcttga acgtcactgg cagaaacgcc cggtggtgtt aaaacgcggc tttaataatt
    23041 ttattgaccc gatctctcca gacgagttgg cgggtctggc gatggaaagc gaagttgaca
    23101 gtcgactggt cagtcaccag gatggcaaat ggcaggtcag ccacggcccg ttcgaaagct
    23161 acgatcatct cggtgaaacc aactggtcat tactggtaca ggcagtaaac cactggcatg
    23221 agccgaccgc cgcgctgatg cgaccgttcc gtgaactacc ggactggcgt attgatgatc
    23281 tgatgatttc tttttctgta cctggcggcg gcgtcggccc gcatctcgat cagtacgacg
    23341 tgtttatcat tcagggtacc ggacgtcgtc gctggcgagt gggcgaaaag ctgcaaatga
    23401 aacagcactg cccacatccg gatctgttac aggtcgatcc gttcgaagcc atcatcgatg
    23461 aagagctgga gcctggtgat attctttata ttccgccagg attcccgcat gaaggctacg
    23521 cgctggaaaa tgcgatgaac tattccgtgg gttttcgcgc gccaaatacg cgggaattaa
    23581 ttagcggatt tgccgattat gtgctgcaac gtgaactggg cggcaactac tacagcgatc
    23641 cggatgttcc acctcgtgct catcctgcgg atgttctgcc gcaagagatg gataaactgc
    23701 gtgagatgat gctcgaattg atcaaccagc cggaacactt taagcaatgg tttggcgagt
    23761 ttatatccca gtcacgtcat gaactggata tcgcgccgcc ggaaccgcct tatcagccag
    23821 atgaaatcta cgatgcgctg aaacaaggtg atgtgctggt gcgcctgggt ggtctgcgcg
    23881 tattgcgcat tggcgacgac gtgtatgcca atggtgagaa gatcgattcc ccgcaccgtc
    23941 cggcactgga tgcactcgcc agcaacattg cgctgactgc ggagaatttt ggcgatgcgc
    24001 tggaagatcc gtcattcctc gcgatgctcg cggcgctggt caatagcggg tattggttct
    24061 tcgaagggta agtttggatt taagccggat gcagcatcac atgccgcatc ccttttcgct
    24121 tacttccgtt gcgccgttaa ctcggcaata cggacgatca cctgcaccgc tttttccata
    24181 ccttccagaa tcacaaactc atgcttacca tgatagttgt aaccgccagt gaacaggttc
    24241 gggcaaggta atcccataaa cgacaactgc gcgccgtcgg taccgccgcg gatcggtttc
    24301 agttccggtt caatatcgca gtcacgcatc gcctgctggg cgatatcgag aatatgcgga
    24361 tgctcaacca ctttctcgcg catattgtag taactgtctt caatcaccaa ttcaatgtag
    24421 caatcaggat gtaacccttt gcccactttt ttggcgatct ccatcatttt acgtttacgc
    24481 gcttcaaact gtttacggtc gaaatcacgg atgatgtagt gcatatcggc ccgttcaacg
    24541 gtgcctttca tgctcgccag gtgatagaaa ccttcatagc cttctgtcat ttccgggctt
    24601 tcatccgccg gaacttccgc atgaatacgt gccgccagcg acagcgcatt taccatcact
    24661 cctttcgccg tgcccggatg aacattgtta ccgacaattt ttatattgac cgatgcggcg
    24721 ttgaagtttt caaactccag ttcgcctacg ccaccaccgt caacagtgta agcccagcgg
    24781 gcatcgaagg catcaacatc aaaatgtttc gcccctttgc ccacttcttc atccggggta
    24841 aaggcgacgc gaatatcacc atgcggaatg tttttctgtt gcaataccgc cagcgcggtc
    24901 atgatttctg caatacctgc tttgtcatca gcacctaaca aggttttacc atcggtggta
    24961 atcaacgtct gacccagtag ctgatgcagc accgggaaca taaccggtga taaaacttca
    25021 tcgccgatac ccagcgcaat atcgccaccg cgatagtttt caacaatttg cggattaaca
    25081 tttttgccgc tgcaatccgg tgaggtatcc acatgagaaa taaagccaat cgccgggata
    25141 tcgccaggga cgttagccgg taacgtcgcc atcaaagtgc ccttctcact taaggtcaca
    25201 ttgataagcc ccatttcttc gagctgctct ttcagcagat gcaataactt ccattggcct
    25261 tccgtgctgg gaacctgtct cacccctgct tttgattggg tatccagaga cacgtagttc
    25321 aaaaatcgct caagtagttt atccatgtag tcaccctcac tttttgtgac aacattatta
    25381 agaagcaaga aaagacaaat attgcgtcag gtcactttta cccctgcaag cgggaatatt
    25441 tatcagcatt accttaatga atataaagct aagccagtaa ttcacaacaa agattggcga
    25501 ttcaagcggt ttgccgtaga attaccgccc tcattaggag tcaccaaggt ggttaaccac
    25561 aaaccccgca tcggtaagcc atccgttgcg tttacatggg acagagtaaa aaattgaata
    25621 aacaaccgag ttcgctttca ccgctggtgc aattggcggg aattcgcaaa tgctttgatg
    25681 gtaaagaggt cattccccag ctggatctga ctatcaacaa tggcgagttc ctcacgctgc
    25741 ttggcccttc tggctgcggt aaaacaaccg ttcttcgcct gattgcaggt ctggaaactg
    25801 ttgattccgg acgcatcatg ctggataacg aggacatcac ccacgttccg gcggaaaacc
    25861 gctatgtgaa cactgttttc caaagctatg cacttttccc ccacatgacc gtgttcgaaa
    25921 atgtggcctt tgggttgcgc atgcaaaaaa cccccgctgc tgaaattacg ccccgcgtga
    25981 tggaagccct gcggatggtg cagctggaaa ccttcgctca acgtaaaccg catcagctct
    26041 ctggtggtca acagcaacgc gtcgccattg ctcgagcggt ggttaacaag cctcgtctgt
    26101 tgttgctgga tgagtcgctc tcagcgctgg attacaaact gcgtaagcaa atgcagaacg
    26161 agctgaaagc gttacagcgt aagcttggca ttacattcgt tttcgtgact cacgaccagg
    26221 aagaagcact caccatgtcg gacaggattg tggtgatgcg cgatggtcgc attgagcaag
    26281 acggcacgcc gcgtgaaatc tacgaagagc cgaaaaacct gtttgttgcc gggttcattg
    26341 gcgaaattaa tatgtttaac gccacggtga tcgagcgact cgacgagcag cgcgtacgcg
    26401 ccaacgttga aggccgcgaa tgtaatatct acgttaactt cgccgttgaa ccggggcaaa
    26461 aactgcatgt tctgctgcgc ccggaagact tacgtgttga agagattaac gacgacaacc
    26521 acgctgaagg gctgattggt tacgttcgtg agcgtaacta caaaggcatg acgctggagt
    26581 cggttgttga actggaaaat ggcaagatgg tgatggtcag cgaattcttc aatgaagacg
    26641 atcctgactt tgaccactct ctcgaccaaa aaatggccat taattgggta gaaagctggg
    26701 aggtcgtact ggctgatgaa gaacacaagt aagttccaga atgtagtgat tgtcactatt
    26761 gtcggttggc ttgtgttgtt tgtctttctg cccaacctga tgatcattgg caccagcttt
    26821 ttgacccgcg acgacgccag tttcgtcaaa atggtcttta cactggataa ctacacgcgt
    26881 ctgctcgatc cgctctattt tgaagtgcta ttgcactcgc tgaatatggc gctgatcgcc
    26941 accctcgcct gcctggtgct gggctatcca tttgcctggt ttctggcgaa gctgccacac
    27001 aaggtgcgtc cgctgctgct gtttctgctg attgttccgt tctggaccaa ctcattgatt
    27061 cgtatctacg ggctgaaaat tttcctcagc accaaaggct atctcaacga gtttttgctc
    27121 tggctgggcg ttatcgacac accaatccgt atcatgttca cgcccagtgc ggtgattatc
    27181 ggtctggttt acattctgct gccgtttatg gtgatgccgc tgtactccag tatcgaaaaa
    27241 ctggataagc cgttgcttga ggcggcgcgc gatctcggtg ccagcaagtt acagactttt
    27301 atccgcatca ttattccact gacgatgccg gggattattg ccggatgtct gctggtgatg
    27361 ctgccagcga tgggcctgtt ctatgtgtcc gacctgatgg gcggtgcgaa aaacctgctg
    27421 atcggtaacg tcatcaaggt acagttcctc aatattcgtg actggccgtt tggtgcagcc
    27481 accagcatta cgctgactat cgtaatgggc ctgatgttgc tggtttactg gcgcgcttct
    27541 cgtttgctga ataagaaggt ggaactcgaa tgatcggtcg actgcttcgc ggcggtttta
    27601 tgaccgctat ctacgcgtac ctgtatatcc caatcattat tttgattgtg aactccttta
    27661 acagctcgcg ctttggcatc aactggcagg gttttaccac caaatggtat agcctgctga
    27721 tgaacaacga cagcctgtta caggcagcgc agcattcact aacaatggcg gtgttttcgg
    27781 cgacgtttgc tacgcttatc ggttcactga cggcagttgc gctgtaccgt tatcgctttc
    27841 gcggtaagcc gttcgttagc ggaatgctgt ttgtggtaat gatgtcacca gatatcgtga
    27901 tggcaatttc gctgctggtg ctgtttatgc tgctgggtat tcagcttggc ttctggtcgc
    27961 tgctgttctc gcatatcacc ttctgcctgc catttgtggt ggtgacggtg tattcgcgcc
    28021 tgaaaggttt tgacgtgcgg atgctggaag cggcgaaaga tctcggtgcc agcgaattta
    28081 ccattctgcg aaaaatcatt ctgccactgg caatgccagc ggtggcggcg ggctgggtgt
    28141 taagctttac cctgtcgatg gacgatgtgg tagtttcttc attcgtcacc ggaccaagtt
    28201 atgaaattct gccgttaaaa atttattcga tggtcaaagt cggcgtatcg ccggaagtta
    28261 acgcgctggc aaccatatta ctggtgctgt cgctggtgat ggttattgcc agccagctta
    28321 ttgctcgtga taaaacgaaa ggtaacacag gggacgttaa atgaaaaaat ggtcacgcca
    28381 cctgctcgcg gcgggtgctc tggcactggg catgagcgcc gctcacgccg atgacaacaa
    28441 cacgctgtat ttctacaact ggaccgagta cgtgccgcca ggactgcttg aacagttcac
    28501 caaagaaacc ggtattaagg ttatctattc gacttacgag tcgaacgaaa ccatgtacgc
    28561 caagctgaaa acgtacaaag acggtgccta tgacctggtg gttccttcaa cctattacgt
    28621 cgataaaatg cgtaaagagg ggatgatcca gaagatcgac aagtcgaagt taacgaactt
    28681 cagcaatctc gatccagaca tgctcaacaa gccttttgac ccgaataacg actactccat
    28741 tccgtatatc tggggtgcga cggcgattgg tgttaacggt gatgcggtgg atccgaaatc
    28801 tgtcaccagc tgggccgatc tgtggaagcc agagtacaaa ggcagcctgc tgttgaccga
    28861 cgatgcccgt gaagtgttcc agatggcgct gcgtaagctg ggctactccg gtaacaccac
    28921 cgatccgaaa gagattgaag ctgcatataa cgagctgaaa aaactgatgc caaacgtcgc
    28981 agcgtttaac tccgataacc cggctaaccc gtacatggaa ggcgaagtta acctcggcat
    29041 gatctggaac ggttctgctt tcgttgcacg ccaggcgggt acgcctattg acgtggtgtg
    29101 gccgaaagaa ggcggcatat tctggatgga cagcctggcg atcccggcaa atgccaaaaa
    29161 caaagaaggc gcgctgaaat tgatcaactt cctgctgcgc ccggatgtgg caaaacaggt
    29221 tgctgaaact atcggttatc caacgccaaa ccttgcggcg cgtaagctgt taagtccaga
    29281 agtggcaaac gataagacgc tctacccgga tgctgaaacc attaaaaatg gcgaatggca
    29341 gaatgacgtt ggcgcagcca gcagcattta tgaagagtat tatcagaagc tgaaagcagg
    29401 acgttaattc agcaagcggg cggtggtatt accgcccgtt ttttagttag caaggatgtg
    29461 gctaatgagt caggacagta aagttttatc ccgctctttc gtcggtattg tggtccgctt
    29521 gtaggcctga taagcgtagc gcatcaggca atgcttcctg ctttataatc cctccagcaa
    29581 cttttcaaca aattctggca ccacctggct tgccgggccg taatatttct cggcaaattc
    29641 attaccaacc tgactcggtt caagattcag ttcaacggtg tgagcaccat gtagtttggc
    29701 ttcgtgtaca aacccagccg ccggatagac atgcccggaa gtaccaatgg caataaagac
    29761 atccgccatc gacaacgcca tataaatttc atccatgccg agtggcattt cgccaaacca
    29821 aacgacgtgc gggcgcaagg gtgccgggaa ctggcaacaa tggcatttat cttctggcgt
    29881 aacgtctccg gtccagtcga gaacctgacc actttgcgaa caacgaactt tcagcaattc
    29941 cccatgcata tgaatgacat tggtattacc tgcgcgttca tgcaggttgt ctatattctg
    30001 cgttaccagc aaaaagcgat cgccgagagc gtcttgcagt ttagccagcg caagatgcgc
    30061 ggcgttaggc tgaatttctg gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg
    30121 caccagttca ggatcgcgat cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc
    30181 ttcccacagg ccatctgcgg cgcgaaaggt acgaatacct gattccgcag aaattcctgc
    30241 ccctgtcagt acgagtactc ttggtttttc cattgcttcc ggcaccactt tatctctgaa
    30301 aaaaatacgc tgacgcaaac gctcgcgcag gcgacgttta tttttacgaa aacgacttaa
    30361 ccgatgaccc cgacgcgaca gcatagcaac ctctgttgtt taatcggtta gatgtaggaa
    30421 ggccgcacca cgcattcctc ccgcatcacc gtggcgcgcg cgttcaatgc gcggaacacg
    30481 agctacaggt aagagatgac aaggcaacct gtccgccagt tgcgttgtga ttgccgggaa
    30541 attcgataag ccaccaccaa tgacgaccag gtcagggtca acaatggtca ggatatttcc
    30601 cagagaaacc gctaataaat ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc
    30661 ttgatcataa agcgcaataa tttcgggagc ctgcaacggt tgatgataat agtgttgata
    30721 cagccacgca aaaccgcgac cagacagata attttcaatg cagccatgct gaccacagcc
    30781 gcagcggcgt aacgggaaat ccaaccccat catggttaac gcatcaaccg gcagacgcat
    30841 atggccaaac tcgccggtaa tgtagctttt accagtaatc ggtttgccgt tgaaaaccag
    30901 cccgccgcca acgccggtgc cgagaattaa ccccatcacc agtggatatt gcgtaaattc
    30961 atcatcccag gcttctgaaa gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg
    31021 atcaagacgt gcgctcaggt cggcacgcag cggtttgccg ctggcagcgg ggacattagc
    31081 ggcatacagc ttaccatctt ctgtttccgg cattcccgga ataccgatgc cgacagaacc
    31141 tttacagcca aaacgttggt cagcttcagc taccagctca cacactgcat ctaaaaatgc
    31201 gtcatagctg tcacgcggtg tcggcacccg cttttcccac tgcaactgcc gaccgctatc
    31261 aaacacgcca agcgcaattt ttgttccacc aatatcaaac ccgtaataca ttgccgctcc
    31321 ttgttttaat gtactgcctt tactggccgc taaggactcg cgcagggtca atattactgg
    31381 cgcgccgcgc cggataccaa cttgccaaaa gactcagcaa caatgctgtg accagtacgt
    31441 agaagacgtc cagccagtgc aattccgatg gcaggaagtc aataaaataa atatcgctgg
    31501 agaggaactg atgaccgatc agcttttcaa tccactcaat aatcggggta agttgcagtg
    31561 aaacaactac gccgataacc accccacaca ggctgccgaa cagccctgcc agcaatccat
    31621 accagacaaa gatggcgcga attaaaccat ctttcgcccc cagcgttctt aatactgcga
    31681 tatcgccact cttgtctttc accgccatca ctaaggtgga gacgatgttg aaacaggcca
    31741 cgccaatcac cagtaccatc gccagataca taatggcgcg gatcatctgg atatcgcgat
    31801 acatatagcc gtaagtacca atccaacttt taatataaac atagctgttg gtcacttcac
    31861 ccgcatcgcg taccagctta ttggcgttga aaacatccgt cattttaagg gcaatacctg
    31921 acacgctgga acccatatca agatattgtt gggcatccgc cagcgggatc atggcaaaac
    31981 tgtgatcgag ttgaccactc aactgcaaaa taccggcaat gtgcaaacgc acacgttttg
    32041 gctgcatcag tttatgctcg ggattcgagt tggggatcat gatcgacacc caatcgccct
    32101 gcttcacttt cagcgcatcc gccacgcctt tgccgatgat aatttgctgt tcgcccgctt
    32161 taaaattgcg ccacgcatcc ccctgaacaa acgagggtaa tgcgctcaga cgctgttcct
    32221 gttgtgggtt aacgcccttc acctggattg cgcgcagatt cgctccactt tccaccagac
    32281 cggtgaaatt gatatacggc gcagcggcgg caatacccgg cactttctgc acgttatcca
    32341 gtgcttcctg ccagttagtc catggctgat ttaccgcttc gatttcgcca tgcggcacca
    32401 ccgccagaat gcggttattc agttcgcgtt caaagccgtt catcgcgctt aagccgacga
    32461 tcaataccgc cacgccgagg gcaatgccaa tggtagaaat gacagagatt agcgacacca
    32521 tgccgccgcg tcgccgtccg cgactaaaac gcaggccaat caataacgat aaaggcatcg
    32581 ccattactcc gcccccatca ggctcagttc cgccgtcaga cgtccatcac gcatttccag
    32641 ttggcggctc atacgtttcg ccagttgcag gtcgtgagtg accaccagga aagcggtgcc
    32701 ctgcaagcga ttcaactccc caagcaactg gaagatgctg tcggcgttac gcgcatcgag
    32761 gttaccggta ggttcatccg ccagtaccag acgcggatta ttgaccagcg cacgggcaat
    32821 cgctacacgc tggcgttcgc cgccagaaag ttcagatggg cggtgattcg cacgatgctc
    32881 cagccccacc gcttttaaca tctccagtgc acagctgttg atttcagcgg gctttttctt
    32941 gccaatcagc agtggcatag ccacgttttc cagggcagta aaatccggca gcaggtggtg
    33001 aaactgataa ataaagccca gcttctggtt gcgcagttct gctttcgccg ctgaagacag
    33061 tttgctcatc ggctgaccgt taaagatcac atcgccggag gttggcgtat ccagcccgcc
    33121 taacaggtgc agcaaggtac ttttaccgga accagagcta ccgacaattg ccatcatttc
    33181 accttcgccc acgctgaaac tgacattgag cagcacatcg gtttgcacac tgccttcctg
    33241 atagcgtttg cacaggttgt cgcattgcaa caggatctta ttcataacgt aaagcctcag
    33301 cgggttgtgt ggcggcagcg cgccatgaag ggtaaagcgt agacagcagc gcgatagcca
    33361 tcgccaccag cgcaataaca atgacctgta aaggttcgat agccaccggc agcgccgcgc
    33421 catcaagcag gacgccgatt atcggcatca gattatttaa ctggctggca agcagtgcgc
    33481 caagcgccgc tccgaggatt gcaccgataa tcccggcgct agccccttgc accataaaga
    33541 ccatcatgat ttgtcgcgga gttaagcctt gcgtttgcag gatcgcgact tcgccctgct
    33601 tctccatcac catcagcccc agcgaggtaa taatgttaaa cgccgcaaca gcgacaatca
    33661 ggctcagcag taagcccatc atattttttt ccatgcgtac ggcctggaac agctcgcctt
    33721 tacggtcacg ccagtcctgc catttgctgc cttcaggcag tttttgctga cttaaagagt
    33781 caactctcag cggctcatcc agccacaaac gccagccggt aatattggct gccggataac
    33841 gcatcagacg cgaggcatcc tcaatattca ccagcatttc atagccatcg acttcactgt
    33901 tggcggcgaa agtgccaatc acattgaaca ggcgctgact tggaatacgc cccatcggcg
    33961 tgaactggct ggcagatggc accatcacgc ggatttgatc accgcgattg acgcccaact
    34021 gtgaggcaag ttgttcgccg aggatgacat tatatttccc cggctcgagg tcagtttgtt
    34081 tcacattgac cagatacggc gttagtggat ctttttgcgc cgggtcgata ccgagcatca
    34141 ccccgaccgc cacgctgcgc gcgctttgca gtaccacatc accggtagta ataggtgcga
    34201 cgcgattaac gccgtccagt ttgactgccg tttccgggag ttgctgcggg ttaagagagc
    34261 catgctcaga agagagaatt gcctgtggca tcaggccaag gatgttgttt tgcagctcgc
    34321 gctcaaagcc gttcatcact gacaatactg tgaccagcgc catcaccccg agggtaatgc
    34381 cgatggtaga aagccaggag acgaaacgac cgaagcgatc cactgcacgc ccacgcatgt
    34441 aacgcaggcc aataaataga gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc
    34501 aaagacacga gtatataagc gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt
    34561 cagggctaat ctgaaaagcc cccgtaaatc aatcggctaa attgccatat tttatcatca
    34621 atcttgccgc ctgatcttaa tctgaaaaat catccaatac agacagatac ccattacacc
    34681 tcgggtcata accagcagga tcaaccgcat gtaaattaaa aaagagcgtg ttgttctgat
    34741 gaaacaaaaa gagctatgga ttaaccagat caaagggtta tgtatttgtc tggtggtgat
    34801 ttatcactcg gtcattacct tttatccgca tatgaccact tttcagcatc cgttatcgga
    34861 agtcctgagc aaatgctgga tctatttcaa tctttacctt gccccctttc gtatgccggt
    34921 ttttttcttt atatccggct atttgattcg ccgctatatc gacagcgtac cgtggggaaa
    34981 ttgtctcgat aaacggatct ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca
    35041 gtggctggcg ttaagtgcgc tgaaccagtg gctggcacct gagcgcgatt taagcaatgc
    35101 ctccaatgcc gcttatgccg attccaccgg tgagttcctg cacgggatga tcaccgccag
    35161 caccagcttg tggtatctgt atgcgttaat tgtctatttc gtggtatgta aaatttttag
    35221 ccgtctggcg ctgccactat tcgccttgtt tgtactgctg agtgtggcgg ttaatttcgt
    35281 tcccacgccg tggtggggaa tgaacagtgt gatacgcaat ttgccttatt acagccttgg
    35341 cgcatggttt ggcgcaacaa taatgacctg tgttaaagag gtgccgttgc gccgccatct
    35401 gctgatggct tctttgctta ccgttctggc ggtcggtgcc tggttgttta ctatctcgct
    35461 gctgttgtcg ctggtatcga ttgtggtgat catgaagctg ttttatcagt acgagcaacg
    35521 attcggtatg cgctccacca gcctgctgaa tgtgattggc tccaacacca ttgctatcta
    35581 caccacccat cgcattctgg ttgaaatatt cagcttaact ctgcttgcgc aaatgaacgc
    35641 agcacgctgg tcgccgcaag tcgaactgac actcctgctg gtttatccct ttgtcagttt
    35701 gttcatctgt actgttgcgg gcttgctggt aagaaaactt tcacagcgtg cattcagcga
    35761 tctgttgttc tccccgcctt cactgcccgc tgccgtcagt tactctcgtt aaaccatcac
    35821 cccagccggg gcgattcaat ttgctaaacc atgtcgatta cggataatga acagcattgc
    35881 ttatcagaga tatgccccca tatgttgagg catatcctaa cgagaatctg acaaccgtta
    35941 tgcctgaaca atatcgttat acgctgcccg tcaaagcggg tgagcagcgt ctgctgggcg
    36001 agttaaccgg cgcagcctgt gcaacgctgg tagcggaaat tgccgaacgt cacgccggtc
    36061 cggtggtact cattgcacca gatatgcaaa atgctctgcg tttgcatgat gaaatcagcc
    36121 agttcaccga tcagatggtg atgaatctgg cggactggga aactcttccc tacgacagtt
    36181 tttcgcctca tcaggacatt atctcctcgc gcctttccac cctttaccag ctaccgacga
    36241 tgcagcgtgg catactgatt gttccggtga atacgcttat gcagcgcgtt tgcccacaca
    36301 gttttctcca cggtcatgcg ctggtgatga aaaaaggtca gcgcctgtca cgagatgcat
    36361 tacgaaccca actggacagc gccggttatc gccatgttga ccaggtgatg gagcacggcg
    36421 aatacgccac gcgcggcgcg ttgctggatc tcttcccgat ggggagtgag ctgccttatc
    36481 gtcttgattt ctttgatgat gaaatcgaca gcctgcgggt gtttgacgtc gacagccagc
    36541 gcacgctgga ggaagtagaa gcgatcaatc tgctgcccgc gcacgaattt ccgaccgata
    36601 aagcggcaat tgaactgttc cgcagccagt ggcgcgatac cttcgaagtg aagcgcgatc
    36661 cggaacatat ttaccagcaa gtgagtaaag gcacattacc tgctgggatc gagtactggc
    36721 agccattgtt cttcagcgaa ccactgccac cgctgttcag ttatttccct gccaatacct
    36781 tgctcgtgaa tactggcgat ctggaaacca gtgccgaacg tttccaggct gacacgctgg
    36841 cgcgttttga gaatcgcggc gtcgatccga tgcgcccgct gttgccacca caatcgctct
    36901 ggctgcgggt ggacgagctc ttctcagagc tgaaaaactg gccccgagtg cagctaaaaa
    36961 ctgaacattt accgacaaaa gccgcgaatg ccaatttagg tttccagaaa ctgccagacc
    37021 tggccgttca ggcgcaacaa aaagcgccgc tggatgcgct gcgtaagttc ctcgagactt
    37081 tcgacggtcc ggtggtgttc tcggtagaaa gtgaaggtcg ccgtgaagcg ctgggtgaac
    37141 tgctcgcacg aattaaaatt gctccgcaac gcattatgcg tcttgatgaa gccagcgacc
    37201 gtgggcgtta tctgatgatt ggcgctgccg aacatggttt tgtcgataag gcgcgtaatc
    37261 tggcgctgat ctgcgaaagc gatctgctcg gtgaacgtgt tgcccgccgt cgtcaggatt
    37321 ctcgccgcac catcaacccc gatacactga tccgtaacct tgcggagctg catattggtc
    37381 agccggtggt ccatctggag cacggcgtcg gtcgctatgc cggaatgacc acgctggaag
    37441 ctggcggcat tactggcgag tacctgatgc tcacctatgc caacgacgcc aaactgtatg
    37501 ttccggtgtc gtcactgcat ctgattagcc gttacgcagg tggcgcggaa gaaaacgccc
    37561 cgctgcataa acttggcggc gatgcgtggt cacgcgcgcg gcagaaagcg gcggaaaaag
    37621 tgcgtgatgt ggcggcggaa ttgctggata tctacgcgca acgcgccgcc aaagagggct
    37681 tcgcgtttaa acacgatcgt gagcagtatc agttattctg cgacagcttc ccgtttgaaa
    37741 ccacgccgga tcaggcgcag gccattaatg cggtacttag cgacatgtgt cagccgctgg
    37801 caatggatcg tctggtgtgc ggcgatgttg gctttggtaa aacagaagtg gcgatgcgcg
    37861 ccgctttcct ggcagtagat aaccacaagc aggtagcggt gttggtgcct accacccttc
    37921 ttgcgcagca gcattacgac aacttccgcg accgtttcgc caactggccg gtacgtatcg
    37981 aaatgatctc ccgtttccgc agcgccaaag agcagacgca aatccttgcg gaagtggcgg
    38041 aagggaaaat cgatattctg atcggtacgc acaaactgct gcaaagtgac gtcaagttta
    38101 aagatttagg cctgctgatt gtcgatgaag aacaccgctt cggggtgcgt cataaagagc
    38161 gcattaaagc gatgcgcgcg aacgtggata ttctgacgct tactgcaacg ccgatcccac
    38221 gtacgctgaa tatggcaatg agcggaatgc gtgacctgtc gattatcgcc acgccgcccg
    38281 cccgtcgtct ggcagttaaa acctttgtcc gtgagtatga cagcctggtg gtccgggagg
    38341 cgatcctgcg tgaaattttg cgcggaggac aggtttatta tctctacaat gatgtggaaa
    38401 acattcagaa agccgccgaa cggctggcag aactggtgcc tgaagcacgg attgccatcg
    38461 gtcacgggca gatgcgcgag cgcgaactgg aacgggtgat gaatgatttc catcatcaac
    38521 gtttcaacgt gctggtttgt accaccatta tcgaaaccgg gatcgacatc ccgacagcca
    38581 acaccattat cattgaacgt gcggatcact tcggtctggc gcagctgcac cagttacgcg
    38641 gtcgcgtcgg acgttcacat catcaggcat atgcatggct gctgacgccg catccaaaag
    38701 cgatgactac cgatgcacaa aaacgtcttg aagcgattgc ctcgctggaa gatctcgggg
    38761 caggttttgc gctggcaacg cacgatctgg agattcgcgg cgcgggtgaa ctgcttggcg
    38821 aagaacaaag cggctcaatg gaaaccatcg gtttctcgct gtatatggag ttgctggaaa
    38881 acgccgtcga tgcactgaaa gccggacgcg agccgtcgct ggaagatctc accagccagc
    38941 aaacagaagt cgagctgcgg atgccgtcgc tattgccaga tgatttcatc cctgacgtga
    39001 acacgcgtct gtcgttctac aaacgtattg ccagcgccaa aacggaaaac gaactggaag
    39061 agatcaaagt cgagcttatc gatcgcttcg gcctgctgcc agatccggcg cgtaccctgc
    39121 tggatattgc ccgtctgcgc cagcaagcgc agaaactggg gatcaggaag ctggaaggta
    39181 atgagaaagg cggggtgatc gaatttgccg agaagaatca cgttaatccg gcctggttga
    39241 ttggtttgct gcaaaaacag ccgcagcatt accgccttga tggtccgacg cgcctgaaat
    39301 ttattcagga tttgagtgag cggaaaacgc gtatcgaatg ggtacgccag tttatgcgtg
    39361 aactggaaga gaacgcgatc gcttaatgta aatcgggggc gtttctgcgc ccccatctgc
    39421 aacatttaca aattctttgc actttcctgc actatccgac actgtcacca tccataattc
    39481 aggcttatct gtttattaca ataaccttat atttattatg tatttttggt gatgatcaaa
    39541 acgcgttttt ctcgctggct aacgtttttt acgttcgccg ctgccgtggc gctggcgcta
    39601 ccggcaaaag ccaacacctg gccgctgccg ccagcgagca gtcgtctggt tggcgaaaac
    39661 aaatttcatg tggtggaaaa tgacggtggt tctctggaag ccatcgccaa aaaatataac
    39721 gtcggctttc tcgctctgtt acaagctaac cccggcgttg atccttacgt accgcgcgcg
    39781 ggcagcgtgt taacgatccc gttgcaaacc ctacttccag acgcgccgcg cgaaggcatt
    39841 gtgatcaaca ttgcggagct gcgtctctat tactacccgc cgggtaaaaa ttcggtaacc
    39901 gtgtatccaa taggtattgg tcagttaggt ggtgacacgc tgacaccgac aatggtgacc
    39961 accgtttcag acaaacgtgc aaacccaacc tggacgccaa cggcaaacat ccgcgcccgt
    40021 tataaagcac agggaattga gttgcctgcg gtagtgccgg ctggaccgga taacccaatg
    40081 ggccatcatg cgattcgtct ggcggcctat ggcggcgttt atttgcttca cggtacgaac
    40141 gccgatttcg gcattggcat gcgggtaagt tctggctgta ttcgtctgcg ggatgacgat
    40201 atcaaaacac tctttagcca ggtcacccca ggcaccaaag tgaatatcat caacactccg
    40261 ataaaagtct ctgccgaacc aaacggtgcg cgtctggttg aagtacatca gccgctgtca
    40321 gagaagattg atgacgatcc gcagctgctg ccaattacgc tgaatagcgc aatgcaatca
    40381 tttaaagatg cagcacaaac tgacgctgaa gtgatgcaac atgtgatgga tgtccgttcc
    40441 gggatgccgg tggatgtccg ccgtcatcaa gtgagcccac aaactctgta aatcctggat
    40501 aaaaaaaatc cccgcgacac ggcatgtcgc ggggaaaaaa tcagcagtag caggcattaa
    40561 tgagggttaa tgcttattta taaattactg ctgttccatg gagggtattc ggaccggtta
    40621 cagaagtaat acggaaagat tttgcgccca tctcatccgc tttttgcgcc agctgctctt
    40681 ccagcgatcc cagatttgtc cccgcgttag cactgatagt accgactttt tgttggcctt
    40741 ctggcgttga ctgaacttcg acagccgcaa agttggcaaa tgacatggag cttaaaatcg
    40801 ccgcagcgat gaggttttta cgtttttcat aatagtggcc ttatgcagat gaatgacgtc
    40861 gcaagaaatt acttaacgat cgataactaa atgatagatg tgatctggat cacatacaag
    40921 atatttttta taacaatcat taattaattt aaaaatcttt aaatttcagt taaatagaaa
    40981 gcaattattt ttatgggatc tggactggtg aacggaggtg attattttta taataacctt
    41041 tcattcacag tcaccaggta caacggcatc atggcaactg actcaacaca atgtgtaaaa
    41101 aaaagccgtg gccgcccaaa agtgttcgac agggatgccg cgcttgataa ggccatgaaa
    41161 ttgttctggc aacacggtta tgaagcgact tctcttgcgg acctcgtcga agcaaccgga
    41221 gccaaagcgc ccacgctata cgcggaattt accaacaaag aggggttatt tcgtgccgtt
    41281 ctcgaccgct atatcgatcg ttttgccgct aagcatgaag cacagctgtt ttgtgaagag
    41341 aaaagcgtgg agtctgcgct ggctgactat tttgctgcca tcgccaactg ctttaccagc
    41401 aaagacaccc cggcaggctg cttcatgatc aacaactgca ccaccctctc cccagattca
    41461 ggagatatag ccaatacgtt gaaatcacgc catgcgatgc aagagcgcac tttgcagcag
    41521 tttttatgtc aacgacaagc gcgcggggaa atcccgaccc actgtgacgt gacacatctg
    41581 gcagaattcc ttaattgtat tattcagggg atgtcgatca gcgcacgcga aggtgcatcg
    41641 ctggaaaaac tgatgcagat tgccggaacg actttgcgtt tatggcccga actggtgaaa
    41701 taagtcgcaa aaaaggcccc tcagcctgag cgaatgaggg gccaaattgc agagtacagc
    41761 cttgttatac tttgttattc aaaatcagtt gcccgttact atctagcggg atctgcgtac
    41821 ccggatcgcg gtccatgcgg attttgccct gctgatcgcc aatcttatag gtcacatcat
    41881 aaccgagcat tttttctgac ttgtcataca ccgttttaca acgctgttgc gtagtcgtgt
    41941 aagtatcgct ttcctggaga gagccctgga tctggttacc ggcatatcca ccacccagcg
    42001 cccccacaac agtggcgaca tctttaccgc gaccaccacc aaactgatgc cctatcacgc
    42061 caccagcaac agcgccgagc accgacccgg taatgcgatt ttcatcctgc accggtcgac
    42121 gatgggtcac tgtgacgttg cgacactcct gacgcggtgt tttaaccgtt tccttgattg
    42181 gggttgcaga aacaacctga gcgtattgcg ggccccgttc aaacacgttc agactggcca
    42241 ctgccgctac gcccaacgca gctgcgacac caatcccgat acccgccaac attgatttat
    42301 tcacgggaac acctccttct ttgcagttat gccaatcatg caagcgaagg agaaaagcag
    42361 ctatcagaca gaagatgaaa agcgcgggaa tgactggaaa tacttaagga ttcggaggag
    42421 tctgaaggaa tgcctgatgc gacgcttaac gcgtcttatc aggcctaccg ctcgtaccat
    42481 ttgtccaggg cggataaggc gtttatacca catccgccag tgtacgtcga ttaatgcaac
    42541 ttcaaacgcg gacggataac gcggttaata ctccccacca gcatcattaa tccggtttta
    42601 aagtaaccat gcagcgcaat ctgatgcatt cggtatagcg agatatatac aaagcgcgca
    42661 attcgtcctt caatcatcat tgagccgcgc gtcaggttac ccatcaggct accaacggtg
    42721 gagaagttcg acagcgatac cagcgaacca tgatctttat actgataatt tttcagcggc
    42781 ttaccgttca tctgcgccag aatgttgttc attgcgcaag tcgccatctg gtgtgcagcc
    42841 tgagcacgcg gcggaacaaa gcccccttcc ggacgcgggc atgacgcgca gtcgccaata
    42901 gcgtaaatgt ctggatcgcg agtggtttgc agcgtcggtt ccaccaccag ctggttgata
    42961 cggttagttt caagaccacc gatatctttc aggaagtctg gcgctttgat cccggctgcc
    43021 cataccatca gatcagcctc aatatattcg ccatctttag tgtgcaggcc gccttcatca
    43081 gcactggtga ccatggtttg cgtcagcacg cgaacgccaa gtttcgttag ctcgttgtgg
    43141 gccgcagcag agatacgtgg cggtaatgca ggcaaaatac gttctcccgc ttctaccagc
    43201 gttacgttca gggcttcgtt ggtcaggcct ttgtaaccgt agctgtgcag ttgcttgatc
    43261 gcgttgtgca attcagcgga gagttctaca cccgtcgcgc cgccgccgac aatcgcaatg
    43321 ttcactttgc catttgcgcc caggttggca gagtatttca ggaacagatt cagcatctcc
    43381 tggtggaagc gacgcgcctg gtgcgggtta tcgaggaaaa tgcagttctc tttgacgcct
    43441 ggcgtattga aatcgttaga ggtgctaccc agcgccatga ccagggtgtc ataggcgatt
    43501 ttacgttccg gaaccaacag ttcacctttc tcgtcgcgta gttctgcaat agtgattgtt
    43561 ttcgcttcac gatcaatatc aatgacggaa cccaactgga actggaaacc atgattgcgc
    43621 gcatgggcca gatagctcaa cgcatcgacg ccttcatcaa gcgagccagt cgccacttcg
    43681 tgcagcagtg gtttccacag gtggctgtgg ttacgatcga ccagcgtaat tttggctttt
    43741 ttcttacgtc ccagcttatg ccccagctgt gttgccattt ccagcccacc agcaccgccg
    43801 ccgacaatca caatcttttt caatggcgta gtcaacgtgc cccccttaaa tttattaacc
    43861 aattgttaat taatggttat taacatagct cttaattaac aacaggttac agcaatgaaa
    43921 atgttcttcg ctattgagaa taacatgaat ggtgcattgg tcataccaaa attgatgtta
    43981 atcaagtttt gttgcttaaa agataggcaa ctttaccggg ggagatcaaa aaatagagtt
    44041 tattgtttat tatttgagca aattcggaag ggtaatatag ggtgaagcgt atatcgaggg
    44101 cgtttggtcg tcctgataag atgcgcagca tcgcatcagg cattgatgct cgactgtcgg
    44161 atgcggcgtg gacgccttat ccgacttaca gaattcggag cgcatcaggt accggcaagg
    44221 ttacctgatg cgacgagggc atttacccga gggttttgaa cgctttaatg cgctgtaaat
    44281 gcggggagat attcttgaat ttgtgcgtct gctcttcgtc ccagacaatc tcgtaataat
    44341 gatgcaactc ttcagatgtc cgctggctgt taagtgcttc atcattacgc gacaaaataa
    44401 ccagacaacg atcgcgattc ttctcgcgga agttggtcac acacttagtg gcaatatcgg
    44461 catactcttc cgggcgatca atcttgcctt ccatgttctc ataagggaac aaattagggt
    44521 tgaagatcac ctggcggata tcgcaaagaa aaccaatccg ttccgcccag tatccgccca
    44581 ggccaacgcc gcaaattagc ggacgctcgt caacgttcag ttgcaacatt ttgtccactt
    44641 ctttaagcag atgctgcata tcatgtttcg gatgccgcgt actgtagctt atcaagcgta
    44701 catccgggtc aataaactgc aattgtaaga ctttctcgtg gttacccgga ctgttagagt
    44761 caaaaccgtg taaatagata atcatcgctt cctcacataa gccagggtta gtgacctact
    44821 ttctcttcct gccagcgttc atgtaactga ttcagacggg tgctgatcgc tttccagcga
    44881 gccgagtcca tcagttcctg tcgcgaaaat gaacctttat gatacaaacg tgtaacacgt
    44941 tctgccttga tcggtgacag attatctaac acgctgacgg cccctttacg attattgcag
    45001 accaggatca tatcgcaacc cgcatccagt gatgcctgcc cgcgttcggc ataactgccc
    45061 ataatcgcgg caccttccat cgataaatcg tcagagaaaa tcacgccgtc aaaccccagt
    45121 tcctgacgca aaacggtttt cagccagtag ggagaaccgc tcgccggacg cggatcaaca
    45181 tcactgtaga tcacatgcgc aggcataatg gcgtcgagtt tattttcgcg gattaaggaa
    45241 ctgaagaccg acatatcttt agcgcgaatc tccgcttgtg gacgtggatc gcacggtgtt
    45301 tctttgtgtg agtctgccgt tactgcaccg tgtcctggga agtgtttccc ggtcgttttc
    45361 attccggctt catgcatacc atcaataaac cggctggcaa ttgccagggc tttttgtgga
    45421 tcggcatgat aagaacgctc gccaatcgcc gcgctgatat gcccgacatc cagcactggc
    45481 gcaaagctga tatcaatatc catagcgatc atttcgctgg ccatcagcca acccgcctct
    45541 tgcgccagtt tgccgccctc ttccattccc aacagcgcag caaaggattg tgctgccggt
    45601 aagcgggtaa aaccttcgcg aaaacgctgc acgcgtccac cttcctgatc taccgccacc
    45661 accagatgat tgcgcgatgc tgcgcggatc tggcgcacca gttcacgtaa ctgggcagga
    45721 tcatgatagt tacgcgtaaa gagaatcagc cctcccacca gcggatgcgc cagtatttca
    45781 cgctcttccg cgtccagttc gtaaccttcg acatccaaca ttactggacc cacactgctc
    45841 tccttattgt tttattaata gctgccgcca ggtgtcatcg gccagcctga taaattgttg
    45901 atcgccggtt tgtcgccagc ggtactcaaa ccaccctcct ttgagcatca gcagccaggg
    45961 aaaccatcgc ctgacctgac gccataattg cgccggataa atcttcgcgc gagtggcata
    46021 gtcattgacc aattgccggt gctgttcagt attttccacc cacaccgccg ccagttccag
    46081 cgcgatatca ccatctccag catactccca gtcgatgagt tttaaccctg atgcgctatg
    46141 cactaaattt ccggcgtgga catccatatg caatggactt aagcgtaaag gtcgtggttc
    46201 ccgcgctttg cgcagacgtt ttaacattcg cagccaaccc acagtccgcc gcgccggatc
    46261 gctttgctgc cagtacagtt ccagtaaggg caacagcgtt attcgccagc caaaacgtgg
    46321 ttgttgatgt agataataca gcaagcctgc cagttcgttg gtatccggca aatacgtttt
    46381 taccgcaccg ggcagatagt cgactaccat ccagtcacgg agatataaat gcggcttcgg
    46441 tgcaatgctt gcgggtagtt gtgataaagc ccgatactgg cgcaagaacg cggactgcgg
    46501 cgcatcagga tcgtgcggct gacgcacaac aaaacgctgg ccctgatgtt caatgagaaa
    46561 actaccgcca ctaagcccac tattaaacgt cgtgacggga tgaaactgcg ggaaaaagcg
    46621 cgacagcaat tcgtcgcgcg tgatgggatt attgctgcga aacggcacct ttacctgacc
    46681 agataatttc gcccgtctgc accagcatca gctgcatttg tagggtcgga gcgttaacgt
    46741 tgccagaggc gcaggagtac agcacgtaat gagcgccgac attgcgggca atgcctatgg
    46801 ctttactacg ggtgcctaaa ctgtcctgcg gcgacaaacc taactgttgc ttagccatcg
    46861 acagttgctg ggcggaaacc agggtaaatt tcccgttatt agccagcgca tttcgcagcg
    46921 tttcggtcgc ttctgcggca ttcagcgaac cgttagtacg gttgttaacg ctatcaacca
    46981 gcaggacgct acccgcagtc accccgtcag ccccaagcat cttactgacc atcggctgca
    47041 ttgcgccatt ccagtcataa tggcgaatat gcggcgcagg cggtgccgtt cgatcttcgt
    47101 gctcaattgg gcctggctgc tgcgggatcg tcggcaccga gggcactgtg gggacaggct
    47161 gttgtggctc ggctggttgt tccggcgctg gtttcacttc ttctaccggt gcaggttcac
    47221 gttgccccac acacccggcg agaaacatcg ccagcgcggt aatcaaggcg tagcgactca
    47281 tttttgtcat caagattcac ccctttacaa atatagataa agtctgactt tgtgcgcccc
    47341 cagaaaattg gcgctgccgt acagcgttac cgccgaatgt gcgggaatgg taacgctgcg
    47401 tggcctttcc agaggatgca tctccagccc tctggcgtca taccagtaaa aacacgataa
    47461 tgaacggtaa cgggttcttg cctttcgtta taaatcgttg aggatgctga aggttgaata
    47521 tcagacgtcg aaaggacggg cttttctgca ctgatgcccg cagccaataa agatgactcc
    47581 atcaccagcg attgctcatc attcaccgga atttccggat gtgaacgaca gcccaccagc
    47641 agtaacaaca ccagagacac cagcccaaag catccttttc tcatcgttac agacctttat
    47701 gcgccagcat tggtcccagc ggacggccac ccaacaagtg catatggatg tggtaaacct
    47761 cttgtccgcc atggcggttg gtattcatga tcagacgata gccatcttcg gcaatacctt
    47821 cttgctcagc aatttttgcc gctacggtga tcatgcgtcc cagcgcctgc tcatgctcag
    47881 ctgacacgtc gttcacagtc ggaatgagaa tattcggaat gatcagaata tgcgttggcg
    47941 cttgcggcga aatatcgcga aacgccgtta ccagatcatc ctggtagacg atatcggagg
    48001 ggatctcacg acgaataatt ttgctgaata tagtttcttc tgccacgaca ttttcctttt
    48061 tcaaatagcc atgcgttgcg ccatgctacg ccgcgccaca ctgcgagtat agagtatggt
    48121 cgagttaaac ctgctctttc aatcttaact cacgatttct taagcaaaaa atcgttttgc
    48181 tgccgtatat atcgccatta ttcccatttc tgcctgcgaa tgcgctgaaa agacattctc
    48241 tgtttggttt acacgacaaa tatgaatgcg tatatttctc atttgcattt gcagcaaaat
    48301 tattcgcaca ctaaatataa ccgctcatca ccctcctccg gcatgagcct gacgacaaca
    48361 taaaccaaga gatttcaaat gctttcaaca caatttaaca gggataatca atatcaagtc
    48421 atcaccaaac cgtcactact tgccggttgc atagcactgg cactattacc ttctgccgct
    48481 tttgctgcac cagccactga agaaacggtg attgttgagg gttcagccac agctccagat
    48541 gatggcgaaa atgattacag cgtaacgtct acctctgcgg gtaccaaaat gcagatgact
    48601 caacatgata ttcctcagtc ggtcactatt gttagccagc agcggatgga agatcagcag
    48661 ttacaaacgc tgggcgaagt gatggaaaac acgctgggga tcagcaaaag tcaggcggat
    48721 tccgatcgtg ctctttatta ttcccgcgga ttccagatcg ataactatat ggttgatggt
    48781 atccccacct attttgaatc gcgctggaat ctgggcgacg cactttctga tatggcactg
    48841 tttgaacgcg tagaagtagt gcgtggcgcg acaggactca tgaccgggac gggtaatcca
    48901 tctgcggcaa ttaatatggt tcgaaaacac gcgacctgtc gtgaatttaa aggcgatgtc
    48961 tcggcggaat acggtagctg gaacaaagaa cggtatgtgg cggatttaca aagcccactc
    49021 accgaagacg gtaaaatccg cgcgcgaatt gtcggcggct accagaataa cgactcatgg
    49081 ctggaccgct acaatagtga aaagaccttc ttctcgggca ttgtcgatgc tgatttaggc
    49141 gatcttacga cgctttcagc cggttacgaa tatcagcgca ttgatgttaa tagcccaacc
    49201 tggggcggtt taccgcgctg gaatactgat ggcagcagca acagttacga tcgcgcacgc
    49261 agtaccgcac ctgactgggc gtacaacgat aaagagatca acaaggtctt tatgaccctg
    49321 aagcagcggt ttgctgatac ctggcaagcg acactgaatg ccacccactc tgaagtcgaa
    49381 tttgacagca aaatgatgta tgtcgatgcc tatgtaaaca aagcggatgg tatgctggtt
    49441 gggccataca gtaattatgg acctggcttt gatcatgtcg gcggcaccgg ttggaacagt
    49501 ggcaaacgta aagttgatgc gctggatttg ttcgctgacg gtagttatga attgtttggt
    49561 cgtcagcaca atctaatgtt tggtggcagt tacagcaaac aaaacaatcg ttcagttcat
    49621 gggccaacat cttcccggat gaaattggca gtttctacaa ctttaatggc aatttcccac
    49681 aagccgactg gtcatcacag agcctggcgc aggacgatac cacacatatg aaatcgttat
    49741 atgctgccac tcgtgtcacc cttgccgatc cgctgcatct gatcctcggc gcacgttata
    49801 ccaactggcg ggttgatacg ctgacttaca gcatggagaa aaaccacacc acgccttacg
    49861 ctggtctggt gtttgacatc aatgacaact ggtcgaccta cgccagctat acctctattt
    49921 tccagccgca aaatgatcgt gacagttcag gcaaatatct ggctccaatc accggtaaca
    49981 actacgagct gggtctgaaa tcggactgga tgaatagccg tctgaccacc acgttagcca
    50041 tcttccgtat tgagcaggat aatgtcgctc agtccaccgg tacacctatc cccggcagca
    50101 acggcgaaac cgcctataaa gcggtggatg ggacagtcag taaaggggtg gaatttgaac
    50161 tcaacggcgc aattaccgac aactggcagc tgacatttgg cgcaacgcgc tatcttgcag
    50221 aggataacga aggaaacgcc gttaatccta atctgccacg caccacggtt aaaatgttca
    50281 ccagctatcg gttgcctgtc atgccagagt tgacagtcgg cggtggtgtt aactggcaaa
    50341 atcgcgtgta taccgacacc gtgacaccgt atgacacctt ccgcgccgag caaggtagct
    50401 acgcgctggt ggatctcttc acccgctacc aggtgacgaa aaacttctcg ttacagggga
    50461 acgtcaataa cctgttcgac aaaacctacg ataccaacat ggaaggttct atcgtctacg
    50521 gcgcaccgcg taatttcagc attaccggca cgtatcaatt ctgaataaca cctgtaaaaa
    50581 aggcagccat ctggctgcct tagtctcccc aacgtcttac ggattagtgg ttacggatgt
    50641 actcatccat ctcggttttc aggttatcgg atttagtacc gaaaatagcc tgaacaccag
    50701 aaccagcaac cactacgccc gctgcgccca gtttcttcag gccagcctga tccactttag
    50761 acacatcagc aacgctgacg cgcagacggg taatacatgc gtcgaggtta gtaatgtttt
    50821 ctttaccacc aaatgcagca accagagccg gtgccatttc gctggtacct gttgcttttg
    50881 catcttcagt cgcgtcttca cgacccggcg ttttcagatc cagtgcttta atcagcacgc
    50941 ggaagatggt gtagtaaaca atcgcataac cgataccgac gatcggtaac agccacagtt
    51001 tgctgctgtt accagacaga acgatgaagt cgatcagacc gtgcgagaac gacgtaccgt
    51061 cacgcatccc cagaagaata cagattggga atgccaggcc tgccagaatc gcgtggatga
    51121 tgtacaggat cggcgcaacg aacatgaaga agaactcgat cggctcggtg ataccggtca
    51181 ggaacgaggt cagcgccgcg gagatcataa taccgcccac tttcgcgcgg ttttctggtt
    51241 tagcagagtg ccagatagca attgcggcag ctggcagacc gtacattttg aacaggaagc
    51301 caccagacag tttacccgca gtcgggtcac ccgccatata acgcggaatg tcgccgtgga
    51361 aaacctgacc tgctgcgttg gtgtattcac caatctgcat ctggaaaggt acgttccaga
    51421 tgtggtgcag accaaacggt accaggcaac gttcgatgaa accgtaaatg ccaaacgcaa
    51481 ctaccgggtt ctggtaagca gcccactgag agaaggtctg gattgcagaa ccaatcggcg
    51541 gccaaatgaa ggacagcaca acgccagtaa agatggcagc caggccagaa atgatcggca
    51601 caaagcgttt accggcaaag aagccaagat actcaggcag cttaatacgg tagaaacggt
    51661 taaacatgta cgctgcaatc gcaccggaga taatccctcc gagtacgcca gtatccgcca
    51721 agtgtttaga ggcgatttct tcagcaggta aatgcagtac cagtggcgca accacggcca
    51781 tggttttaac catgatgcca taggcaacaa ctgcggccag cgcggatacg ccatcgttat
    51841 tggtaaagcc gagggcgaca ccgatcgcaa aaatcagtgg catgtttgca aagacggaac
    51901 cgcctgcttc tgccataaca tgcgatacaa cggcgggcag ccagctgaaa ttcgcggaac
    51961 cgacgcccag cagaatacct gcgataggta gtacggatac cggcagcatc agcgatttac
    52021 cgaccttttg caggttagca aatgcattct taaacataat tgagagtgct cctgagtatg
    52081 ggtgcttttt ttacgttctc acgcgtggca aggggggaga gcctcgccgt gtacagggca
    52141 tctaagcgcc ctttatttat tacacagagt aaaataattc agtgccaatt tgtttgacgg
    52201 ctatcacgtt tcaacttcag atcattggag aagttgcaca ttttgacaaa acatatgtca
    52261 caatatttca aacgcggtta ttcaccgccc aattttcggc ggtgaacttt actcgtttta
    52321 gccattaatt acgagcttta aaaaaactca ttcaacggat ggattgaagg cgggaagcgt
    52381 cgatgtgaaa cagacgggcg aagttatcgg tggttacctg cgccagttct tcaacggcaa
    52441 cacctttcaa cacagccatg tattctgcaa cgtcacgaac catcgcaggt tgattctctt
    52501 ttcctcgatg cggtaccggc gcaaggtaag gtgagtcagt ttccaccagt aatcgatcca
    52561 gggggacata acgcgcagca tcgcgcagtt gctccgcatt acggaaggtc acaatgccgg
    52621 aaaaggagat gtaaaatccg agatccagta atttacccgc cgtttctctg tcctctgtaa
    52681 aacagtgtag tacgccaccg caatccgtca ctttttcttc gcgcagaatt gccagcgtat
    52741 cggcacgggc gtcacgggta tggacgataa ccggcttgtt cagttcacga ccaatctgga
    52801 tatgatggat gaaggactct tgctgacgta ctttagtttc cggtgtgtaa taataatcca
    52861 gcccggtttc acccagcgct acaacaccct cttctgccgc cagacggcgt aaatcttcta
    52921 catcgtaggg atcattctgg ttgagtggat gcacgccaca agaaaatacg acgttgtcac
    52981 gttcgcctac cagatcccgc atatgtaaat aacccggtaa tgttgtggcg actgccagac
    53041 aaaatttcac atcgcgtgcg gcggctttcg ccagaacgtc atccacgtcc ttatgcaaag
    53101 attcataatc cagaccatcg agatggcagt gtgagtcgac taaaaacatg atgtctctct
    53161 taaagatgag gaaccggtaa cacaacgccc ggttgcaggt aatgctcaat acgcagtaaa
    53221 agatcggtga tgagaagctc gcggttgatg cctgtgacag acattaactg ttcacgaatg
    53281 tggcaaacat cccccagtat agcctgcagg cacgagggag aaagatggtt tgccagttcg
    53341 gcgaccaggc ccggcacatc aacattggtc acctgcgcag cgccatgatg gcgttttagc
    53401 gcatccatca gcaacgttgc cagccagtgt aaacgcgccg gagcttgttc atgattaagg
    53461 gccgccagca gcgaatacca gtcgcccgat ggcacgctat atgccaacgc ctgacacaat
    53521 gtttcacgag cctgccagtt atctccctga aacaacgcca gtgccgcgcc aggcgaaccg
    53581 gcgcttaagc gcaatgcggc aagtaatgca tcctgtgaca ttgtcacttc gcgtgaaagc
    53641 caagtcacgg cgtactgttc tggaggcggc gcaaggtaat gtaaccgaca acgactacgt
    53701 aatgttgcca gtaaacgttc aggctcgcgg gtagccagga aaaaccaggt ttctgctggt
    53761 ggctcttcaa gcgttttcag caatgcgtta gccgcggcgt cggtcagtaa ggcagcatcg
    53821 gttacccaga caactttcgc accacctagg cgtgcgtgct cattcagctt ttcggtgacc
    53881 tcacgtaccg catcaatgcc cagcgtattt ttcccttttt cgggagccag ggtgtagtaa
    53941 tcgggatgcg tgccagcctg catcaactga catccacgac agtgaccgca acttttgtgg
    54001 ccctgcggtt gttggcagag taaataacgg ctcagggcgt agattaaagc atcatcgccc
    54061 atgcccggta acgcctgaat gagtagcgca tggtgacctc ttccggcctg atagctggct
    54121 accagttttt cgaaatcagg tcgtaaccat ggataccatc tcatgcgtcc aactccttca
    54181 cccagtgggt cacggtagtg cggattgcat ccatcacggc ctccagcggc tgggtggcat
    54241 caatggtatg aatactttta tcttgtgcag ccagttccag atagcgggcg cgggtgcgat
    54301 taaagaaatc gaaagactct tgctcaatac gatccagctc gccgcgcgca cgcgcgcgtt
    54361 ttaagccaac ttccggggta acatcgagat agagcgttaa gtctgggcga aaatccccga
    54421 gaacagcatc acgcagtgtt gccagcatat gttggtcaat accacgtcct ccgccctgat
    54481 acgcctgagt ggaaagatcg tggcgatcgc caatcaccca ggtgccgtta gccagcgcag
    54541 gtttgatgac cgtttctacc agttgaacgc gcgcggcata aaacatcaga acttcggctt
    54601 tatcggtaat gacttcatcg cctaccgatt tgatatccag caccaggctt cttaactttt
    54661 cggcaagttg cgtaccgcca ggttcccgag tgaaaaccat gtcgcggata cccagttgct
    54721 cgagcgtctc aaccaccaca ttacgcgcgg tagttttgcc tgcgccttcc agcccctcaa
    54781 tgacgatata cttactgcgc atttttttcc ttaagcactt tcagataatc ctgcacagac
    54841 ttgttatgac tggcaagatt ggtattaaac gtgtgaccac ctttaccatc ggccacaaaa
    54901 tagagatacg gcgtttttgc cggatgcgca gcagccttca gcgaatccgc ccccggcgta
    54961 gctatcgcac ccggcggcaa accggtaatg gtataggtgt tatacgctgt cggcgtttcc
    55021 aggtctgcac gagaaagttt gccattataa cgctctccca tcccgtaaat cacggttggg
    55081 tcggtctgca ggcgcatacc aatgcgtaaa cggttgataa ataccgaggc aacctgatcg
    55141 cgttcactgg caacggcggt ttctttttcg ataattgatg ccatcgtcac cagctgattt
    55201 ttatctttat aaggcagacc gtccgcacgc ccttcccagg cgctatcgac cgctttcacc
    55261 attttcttgt gaactcgctt gagtaacgcg acatcggtgg tattggcggt atacatcaag
    55321 gtgtctggcc agaaccaacc ttcaatccac tccgggtttt ccagttcaag tgcctgcgct
    55381 acggtggcgt acttatcgtc actcagcgtg tgcttgatat acggagcctc acgcaattgc
    55441 ttgaggtagt cgctcagacg catcccttct accagtcgca gcgggaactg tgcttcttta
    55501 ccgctttcca acaatttcag catctcgcgc acggtcatct gcggtgtaaa gcggtaagtc
    55561 ccggctttaa agtgagaaag atccggttcg atacgcagca gccattgaaa aacccgtggg
    55621 cgattgatga tcttatcagc ataaagctgt tcaccgagcg ccagacgtcc ggtccctggc
    55681 ttcagggtaa atatcgtctc ttctttgata agcaatttgc tgtcggcaag atggcgaacc
    55741 ttccagacgc ccacaccagc ggcgataccc agtaccacca gcaataacaa gataatcaat
    55801 aacacttttt tcatgactaa ttcgggcgct cacaaagtgg ggctaaatat tcatataacg
    55861 ttgctgacga aaaggtgaca tcgccacagg cacgtacggg catcactggc attaacgcat
    55921 tacaaataac catctcatct gcctgcaacg cctcctccag cgaggcttgc acttcgacaa
    55981 gctgataaga ggattgtgcc agtaaacgga tacagaattg tcgcataatg ccgttaacac
    56041 ctgcctgatc cagtcgcggc gtataaacta cattgccctt ccgccagaac aaattagccg
    56101 cacagcattc cgtaacccac ccttcgctgt caaggaccag cgcctcatca gcgtttgtct
    56161 gctcaagatg agagcgaatc aatacttgct caagccgatt aagatgttta atacctgcaa
    56221 gatgaggatt gcgccccagc cgcaccgggc ttagcgccaa cgtcatccct tcgttacgca
    56281 aacggtcgta atgtgcagga taagccgtaa cggagagaat ccgcgttgct ggtccgctgt
    56341 tcaatgtgct gtaccctcgc ccgccactac cgcgactgat cacgactttc agcacaccat
    56401 tttgctgttc tgctgccagc gttttcatct cttgttcaag ctgaggccag aagtcacagg
    56461 aaatcatcaa ccgctgacaa gcatcctgta gtcgcaggat atgcgccgat aacaaactga
    56521 ctttaccgtc gataactctg gcggtggtaa aacaaccatc accaaactgc gttgcccgat
    56581 cgcttactgc cagcgattcc tgcttataac cgttaattaa gaacataccg gctccttatc
    56641 agagaattgt tagtgtggca gcatgttcac tacggaacaa gtcggaataa aagctaagaa
    56701 aaaaggcccg caagcggacc ttttatatgg gtggaaaatg acaacttaga tctttttaaa
    56761 gatcaaagaa ccattagtgc caccgaagcc gaaggagtta cacagcgtgt attccattcc
    56821 gctgacctga cgtgcttcat gcggtacgaa atccagatcg caaccttcat ccgggttatc
    56881 caggttgatg gttggcggaa cagcctgatc gcgcagcgcc aggatggagt agatagattc
    56941 tactgcaccc gccgcaccta acaggtgacc ggtcatagat ttcgtggagc ttaccaacac
    57001 acggcttgca gcttcaccga agatggtttt caccgcctgc gcttcagctt tatcgccagc
    57061 cggcgtagaa gtaccgtgcg cgttaacgta gccaatctga ctcgcttcaa tgcctgcatc
    57121 acgcagagca tttgccattg ccagagctgc gcctgcgcca ttttctggcg gtgacgtcat
    57181 atgataagca tcgctgctca taccaaagcc gacgagttca gcgtaaattt tcgcaccgcg
    57241 ttttttcgcg tgttcgtact cttcaagtac cagcataccg gcaccatcgc ccagtacgaa
    57301 gccatcacgc tctttatccc acgggcggct cgccgcttgc gggttatcat tgcgggtaga
    57361 taatgcacgt gccgcgccaa aaccaccaac gcccagcggc gtactggctt tctctgcgcc
    57421 acctgcaacc atcacgtcag catcgccata cgcgataata cgcgcagcat ggccaatgtt
    57481 gtgcacgccg gaagtacagg cggtcgcgat agagatgctt gggccacgca ggccatacat
    57541 gatagtcaga tgacctgcca ccatgttcac aatcgttgac ggaacgaaga atgggctgat
    57601 cttacgtgga ccaccgttca tcagagatgt gtggttttct tcgatcagtc cgaggccgcc
    57661 aatcccggag ccaattgcgg caccaatgcg ggttgcgttc tcttccgtta tttcaaggcc
    57721 agaatcctgc atggcctgaa cgccagcgac aattccatat tgaatgaagg catccatctt
    57781 gcgctgttct ttgcgcgaga taatgtcctc acagttaaaa tcctttacta agccagcaaa
    57841 tttcgttgca taggcgctag tatcgaaatg gtcgattagg ctgatgccac tctgaccggc
    57901 aagcagagct ttccaggtag actctacggt attgccgaca ggagacaaca tgcccagtcc
    57961 ggtcacaact acacgacgct tagacacgtt tgtcctccaa ggagggaaaa atgattctag
    58021 tgggacaaaa agataaaact caggcggtcg aacgaccgcc tggagatgtt cacttacgcc
    58081 tggtggccgt tgatgtaatc aatggcagcc tgaacggtgg tgattttctc agcttcttcg
    58141 tccggaatct cagtatcaaa ctcttcttcc agagccatta ccagctcaac ggtgtcaaga
    58201 gaatccgcgc ccaggtcttc aacgaaagaa gcattgttgg taacttcttc ctgcttaacg
    58261 cccagctgtt cgccgataat tttcttaacg cgttcttcga tagtgctcat actcttaaat
    58321 ttcctatcaa aactcgcttt cgcgatggtt ttcgtagtgt ataaaatgtt gaaaaatttg
    58381 caactaaatc ccggcaggtc ttaccacgat tttacgttat tttgaggcct taccccccaa
    58441 taacgcaaat cattttgcac taattattac tcataaccac gcagactgcg cgcaacatga
    58501 gcattttgtg caaatcgcgg tcagaccatg tacatcccgc cgttcacatg caaagtttca
    58561 cccgtgatgt aagctgcttc gtcggatgcc aggaatgcaa ccgcgttggc gatttcctgt
    58621 gcgccgccga ggcgacccgc aggaacctgc gccaggatac ccgcacgctg gtcatcgctc
    58681 agcgcacgtg tcatgtccgt ttcaataaag cccggagcaa caacgtttac agtaataccg
    58741 cgtgacgcaa cttcgcgcgc cagtgattta ctgaagccga tcaagcccgc tttcgccgca
    58801 gcgtagttgg cctgaccgcc atttcccatg gtaccaacca cagaaccgat agtgataata
    58861 cgaccatgac gctttttcat catagcgcgc attaccgctt ttgacagacg gaaaacagat
    58921 gaaaggttag tttcgataat atcgttccac tcttcatctt tcattcgcat taacaggtta
    58981 tcacgagtga taccggcatt attgaccagg atatccactt caccaaattc tgcgcgaatt
    59041 ttttccagaa cagattcgat agatgccggg tcggtcacat tcaacatcag acctttgccg
    59101 ttggcaccta aataatcact gatcgcctga gcgccatttt cactggtcgc agtgccaata
    59161 actttcgcgc cacgggctgc gagcgtttca gcaattgcgc ggccaattcc gcggcttgca
    59221 ccggttacca gtgcgatttt tccttcaaaa ttcatgattt tcctctttta aagctcgagc
    59281 gccgctgcca tcgctgaagg ttcgttcagc gccgaggcgg tcagggtgtc gacaatgcgt
    59341 ttcgtcaggc cagtaagcac cttgcccggg ccgacttcat agagatgttc tacgccttgc
    59401 gctgccatgt actcaacaga cttcgtccac tgaaccgggt tatacaactg acgtaccagt
    59461 gcgtcacgga tggcatcacc attggtttcg catttcacat caacgttatt cacaacagaa
    59521 actgttggtg cgttaaaggt gattttcgct aattctactg ccagtttgtc ggctgctggt
    59581 ttcatcagcg cacagtgaga cggtacgctc actggtaacg gcagcgcgcg ttttgcgcca
    59641 gcagctttac aggcagcgcc agcacgctca accgcttctt tatgaccggc aataaccacc
    59701 tgtcccggag agttaaagtt taccggagaa acgacctgac cttctgcagc ttcttcacac
    59761 gctttcgcaa taggcgcatc atccagaccg atgattgccg ccatagcgcc cgtgccttcc
    59821 ggtacggctt cttgcatgaa cttaccgcgc atctcaacca gacgcaccgc atcagcgaaa
    59881 tcaatcacgc cagcgcaaac cagcgcggag tattccccca ggctgtgacc ggccatcatt
    59941 gccggtgctt taccgccctg ctgctgccat acgcgataca gcgcaacaga tgcagtcaac
    60001 agcgctggct gggtttgcca ggttttattc agttcttcag ctgacccctg ctgggtcagc
    60061 gcccacaggt cgtagcccag cgccgcagaa gcttcagcaa acgtttcttc gacaattgga
    60121 tagctcgccg ccatatcagc cagcattcca acggtttgag aaccctgtcc agggaacaca
    60181 aatgcaaatt gcgtcatgtt ttaatcctta tcctagaaac gaaccagcgc agagccccaa
    60241 gtgaatccac cgccaaaggc ttcaagcaga accaactgcc ccggcttaat gcgcccgtcg
    60301 cgtacagctt catccagcgc gcacgggaca gaggccgcag aggtattacc gtggcgatcc
    60361 agcgtcacca cgacattgtc catcgacata ccgagttttt tcgccgttgc actgataata
    60421 cgcaggttag cctgatgcgg aaccagccag tccagttgag aacggtcaag attattcgcc
    60481 gccagcgtct catcaacgat gtgcgccagt tccgttaccg caaccttgaa gacttcgttg
    60541 cccgccatcg tcagatgaat tgaattctct ggattcacgc ggtcggcgtt tggcagcgtc
    60601 agcaattcac cataactacc gtcggcatgc agatgggtgg aaatgattcc cggctcttca
    60661 gaggcagcca gcaccgcagc gcccgcgcca tcgccaaaaa taataatagt cccacgatct
    60721 gttggatcgc aggtgcgcgc cagtacatcg gaaccgacga ccagagcata cttcaccgcc
    60781 ccagatttca cgtattgatc ggctacgctt aatgcatagg tgaaacctgc acaggctgct
    60841 gcaacgtcaa atgccgggca acctttaatg tccaacatgc tttgaatctg acaagctgcg
    60901 ctcgggaaag cgtgcgtagc agaagtcgtt gccacaacga tcaggccaat ctggtctttc
    60961 tcaatgcccg ccatctcaat tgcgcgtgtc gccgcttcaa agcccatggt tgaaacggtt
    61021 tcgtttggcg cggcaatgtg gcgttcgcgg ataccggtac gagtgacaat ccactcgtca
    61081 gaggtgtcca ccattttttc caaatcggcg tttgtccgca cttgttcggg cagatagctg
    61141 ccagtaccaa taatcttcgt atacatgtac gctcagtcac ttttcggtta tataccgtca
    61201 cttgcaaact gcgagttcgc tggcagcgtc ctgctaccgc agagttccgc ttttgccacc
    61261 gtccagcagc tcaaaaccag ctgggtatac agattccagg cgagcggcaa ttcgctgagg
    61321 aacttgtcgc tgcaccgcct gcactgcctg ttcaatcgcg accgcaaaag ctcgctgatt
    61381 ggctgcacca tgacttttta tcaccgtgcc gcgcaatcct aacagacagg cgccgttata
    61441 ctggtcgggg ttgaggtgac tgaatcgcct cgtcaggctc ttttgtagcc aacgctttaa
    61501 taacagtagc caccacgacc gttttttccc ttcaccctga gatttcagca gagaaaggaa
    61561 catcctgaca acaccttcca tcgtctttaa cgtgacattt cctgtaaagc cgtcacaaac
    61621 cagcacatct gtcttgccag ttaacaactc attggcttca agatagccga tataattgat
    61681 agaagggatt gttttaagca ccgctgaggc atcccgaata ctgtcaagac cctttacttc
    61741 ttcttcacca atattgagca acgccacgcg aggattggga atttccacca cctcttcagc
    61801 cagaactgag cccataatgg caaattgcac caacattgtg ctgtcacaat cgacgttggc
    61861 ccctaaatcg aggaccaccg ttttgccctt ttgctgatgt ggtaataccg tcaccagcgc
    61921 cggacgctca atcccctcca ggggcttgag taataatttt gccagcccca tcagtgcccc
    61981 ggtattaccg gcactgacac aggcttgcgc tcgaccttct ttcaccagct ccagggccat
    62041 acgcattgaa ctcccacggc tggcgcggat agcttgcgaa ggccgggcat cactggcgat
    62101 aactgactgc gcaggaataa tctgcagacg cgaacgttgt tcaaagtcag ctttagcaag
    62161 taatggcgtg atggcgtcgg gattgccgac taaaagaaga gtgagttgcg aattagaatt
    62221 cagtgcctgc aatgctgcag gcactgtcac ggaagggcca aaatcccctc ccatgacatc
    62281 taacgccagg gttagacgtg tcaaggtatc gtcgccgccc ggtttggtct ttccccagtt
    62341 gcctggggaa aatcctcact aagcttcatc acgcagacgc gtgattactt agcgatgacc
    62401 ttgcggccgc ggtagtaacc gtcggcagtg atgtggtgac gcaggtgttt ttcaccagaa
    62461 gttttgtcta cagacaggct ggtgactgcg gtcagcgcgt catgggaacg acgcatgcca
    62521 cgtttggaac gggttggttt attctgttgt acggccatgg accttactcc tcaattacgg
    62581 tttatccccg atccaacggg gagcaccaat tacttacgct ttaagctggc taatacggca
    62641 aatgggtttg gcttttgcac ttcttcaggc agttcaccaa agaccatgtc cgcttcggac
    62701 acttcacagt gttcagaatc atgcaccgga actaccggca aggcgaggat gatttcatct
    62761 tcaaccattg caagcagatc gatttcaccg aattcgttaa cctcaatcgg ttcatacgct
    62821 tccggcagtg cttcagcctg ttcgtctgaa cgcacaggac taaaacaata cgttgtgtag
    62881 acctgatgag taaacggctt cccgcaacgc tgacactcga gcgttaccgt caccttcgca
    62941 tcgccgttta acactgcgag acgttggtta tcgatagcga acgacatgga gcattccaca
    63001 tcactgtcca cactgactac ggattcggcg acgcgctcaa cctgatcagg ggtatagata
    63061 ccctggtaat caaggcgttt ttgagccgta cgaaccggat cgagagtcag gggtaatttt
    63121 accttttgca tagggcgcgc atattaactt tgtaacgtca tagagtcaaa gaaaaaggca
    63181 gcctgtggtt gccttttgcc aataattcgc acacattgcg ggttactgct ttattttcgt
    63241 tcagtgctga taacgaacat caacctgcaa aacgaagcgc atagtttaaa atggctatca
    63301 tcaatacgct atatctgatg gaaaaaaata tgcctaaact tattttagcc tccacctcgc
    63361 catggcgtcg cgctcttctg gaaaaactgc aaatctcttt tgaatgtgca gcacctgagg
    63421 tcgacgaaac cccacgcagc gacgaatcac cgcgacagtt ggtgcttcga ctggcacaag
    63481 aaaaatcgca atctctggcg tcacgttatc cggatcattt aattattggt tcagaccagg
    63541 tatgtgttct tgatggcgaa atcaccggta aaccgttaac ggaagaaaat gcccgtctgc
    63601 aattacgcaa agccagcggc aatatcgtca ccttctatac tggactggcg ttgtttaatt
    63661 cggcgaatgg gcatctacaa acagaagtgg agccttttga cgtccatttc cgtcacctaa
    63721 gcgaggcgga gattgataat tacgtgcgta aggagcatcc cctgcactgc gcgggtagct
    63781 ttaagagtga aggatttggc attacgctgt ttgagcgttt agagggacgt gaccctaaca
    63841 cgctggttgg tttaccgcta atcgcgctgt gtcagatgtt acgtcgggaa gggaaaaacc
    63901 cgctgatggg atagttgttt gcgccggatg cggttacgtc gcatccggca atcggtgcag
    63961 gcggcctgat gcggcgtaaa cgccttatca ggccaaccgc ccattatcag gcttatatgc
    64021 ttgtattagc gcgcgttacg cagcttttgc aaacaacgct tcaaaccttc atccatcggc
    64081 gcttcgatac gcatcacctc accggtcccc ggatgggtaa acttcaacgc tgcggcgtgc
    64141 aggaacagac gatttaatcc cgtgcctgct tcagtgagct gtctgtcaaa ttcacggtca
    64201 ccgtagcgat cgtcaaaggc aatcggatga cccgcatact gtgtatgcac acggatctga
    64261 tgagtacgtc cggttaccgg gctacaacgc accagggtgg caaatgcata gcgttcttcc
    64321 actttaaagc gtgtttccga cggtttgcct tcctgactga cacgcacgat acgttcgccg
    64381 ctttgcagaa tatttttcaa taacggcgct tgaacgctct tcacatgcga ctgccactga
    64441 ccgcgcacca gcgccaggta atctttttgc attccttttt cacgtaattg ctcatgcaga
    64501 gaacgcaacg ccgagcgttt tttcgctacc agcaacacac ctgaagtgtc ccggtcgaga
    64561 cgatgaacca gttcaaggaa ccgcgcttcc gggcgcaacg cccgcaaacc ttcgataacg
    64621 ccgaagctta aaccactgcc gccatgtacc gccgtaccgg aaggtttatt cagcaccagg
    64681 atatgatcat cttcatataa gatgacgtcc gccagcgccg ccaccttttg cagatgtggc
    64741 gaaaccgcct cttcttcccg ctcagcaacg cgaaccggtg ggatacgcac ctcatcaccc
    64801 gcttcgagtt tatattcagg cttaatacgt tttttgttca cccgcacttc acctttacgc
    64861 aaaatacggt aaatcatact ttttggtacg cctttcaatt gggtacgcaa aaagttatcg
    64921 atacgttgcc ccgcttcgtc agcggtgata gcaacaattt ttacggatgg agtctctgtt
    64981 ttcatggtgg gcgattctaa ttagccaaca ggattcgcgc cactcatttt tctatgctta
    65041 tatttagttt tgcaccttat tacttcacta cgtgatcact ttattgatgg ttattaaacc
    65101 aatcaccagc aagaagtgaa aaaactgtga gtaagcgggt gataaatggt aaaagtcatc
    65161 ttgctataac aaggcttgca gtggaataat gaggccgttt ccgtgtccat ccttgttaaa
    65221 acaagaaatt ttacggaata acccattttg cccgaccgat catccacgca gcaatggcgt
    65281 aagacgtatt gatctttcag gcagttagcg ggctgcgggt tgcagtcctt accggtagat
    65341 ggaaatattt ctggagagta atacccagtc tgtttctttg ataattgcgc tgtttttccg
    65401 catgaaaaac gggcaaccga cactctgcgc ctctttgagc tgacgataac cgtgaggttg
    65461 gcgacgcgac tagacacgag gccatcggtt cacacccgga aaggcgttac tttgcccgca
    65521 gcttagtcgt caatgtaaga ataatgagta agttacgatg aaaagaatgt taatcaacgc
    65581 aactcagcag gaagagttgc gcgttgccct tgtagatggg cagcgtctgt atgacctgga
    65641 tatcgaaagt ccagggcacg agcagaaaaa ggcaaacatc tacaaaggta aaatcacccg
    65701 cattgaaccg agtctggaag ctgcttttgt tgattacggc gctgaacgtc acggtttcct
    65761 cccactaaaa gaaattgccc gcgaatattt ccctgctaac tacagtgctc atggtcgtcc
    65821 caacattaaa gatgtgttgc gtgaaggtca ggaagtcatt gttcagatcg ataaagaaga
    65881 gcgcggcaac aaaggcgcgg cattaaccac ctttatcagt ctggcgggta gctatctggt
    65941 tctgatgcca aataacccgc gcgcaggtgg catttctcgc cgtatcgaag gcgatgaccg
    66001 taccgaatta aaagaagcac tggcaagcct tgaactgccg gaaggcatgg ggcttatcgt
    66061 gcgcaccgct ggcgtaggca aatctgctga ggcgctgcaa tgggatttaa gcttccgcct
    66121 gaaacactgg gaagccatca aaaaagccgc tgaaagccgc ccggctccgt tcctgattca
    66181 tcaggagagc aacgtaatcg ttcgcgcctt ccgcgattat ttacgtcagg atatcggcga
    66241 aatccttatc gataacccga aagtcctcga actggcacgt cagcatatcg ctgcattagg
    66301 tcgcccggat ttcagcagca aaatcaaact gtacaccggc gagatcccgc tgttcagcca
    66361 ctaccagatc gagtcgcaga tcgagtccgc cttccagcgt gaagtccgtc tgccatccgg
    66421 tggttccatt gttatcgaca gcaccgaagc gttaacagcc atcgacatca actccgcacg
    66481 cgcgacccgc ggcggcgata tcgaagaaac tgcgtttaac accaacctcg aagccgccga
    66541 tgagattgct cgtcagctgc gcctgcgtga cctcggcggc ctgattgtta tcgacttcat
    66601 cgacatgacg ccagtacgcc accagcgtgc ggtagaaaac cgtctgcgtg aagcggtgcg
    66661 tcaggaccgt gcgcgtattc aaatcagcca tatttctcgc tttggcctgc tggaaatgtc
    66721 ccgccagcgc ctgagcccat cactgggtga atccagccat cacgtctgcc cgcgctgctc
    66781 cggtaccggt accgtgcgtg acaacgaatc gctgtcgctc tctattctgc gtttgatcga
    66841 agaagaagcg ctgaaagaga acacccagga agttcacgcc attgttcctg tgccaatcgc
    66901 ttcttatctg ctgaatgaaa aacgttctgc ggtgaatgcg attgaaacgc gtcaggacgg
    66961 tgttcgctgc gtgattgtgc caaacgatca gatggaaacc ccgcactacc acgtgttgcg
    67021 cgtgcgcaaa ggggaagaaa cgccgacctt aagctacatg ctgccgaagc tgcatgaaga
    67081 agcgatggcg ctgccgtctg aagaagagtt cgctgaacgt aagcgtcctg aacaacctgc
    67141 actggcaacc tttgccatgc cggatgtacc gccagcgcca accccagctg aacctgccgc
    67201 gactgtcgta gccccagcac cgaaagctgc aacggcaaca ccagcagctc ctgcacaacc
    67261 tgggctgtta agccgcttct tcggcgcact gaaagcgctg ttcagcggtg gtgaagaagc
    67321 caaaccgacc gagcaaccaa caccgaaagc agaagcgaaa ccggaacgtc aacaggatcg
    67381 tcgcaaacct cgtcagagca accgccgtga ccgtaatgag cgccgcgaca cccgtagtga
    67441 acgtactgaa ggcagcgata atcgcgaaga aaaccgtcgt aatcgtcgcc aggcacagca
    67501 gcaaactgcc gagacgcgtg agagccgtca gcaggctgag gtaacggaaa aagcacgtac
    67561 caccgacgag cagcaagtgc cgcgtcgtga acgtagccgc cgccgtaacg atgataaacg
    67621 tcaggcgcaa caagaagcga aggcgctgaa tgttgaagag caatctgttc aggaaaccga
    67681 acaggaagaa cgtgtacgtc cggttcagcc gcgtcgtaaa cagcgtcagc tcaatcagaa
    67741 agtgcgttac gagcaaagcg tagccgaaga agcggtagtc gcaccggtgg ttgaagaaac
    67801 tgccgctgcc gaaccaattg ttcaggaagc tccagctcca cgcacagaac tggtgaaagt
    67861 cccgctgcca gtcgtagcgc aaactgcacc agaacagcaa gaagagaaca atgctgataa
    67921 ccgtgacaac ggtggcatgc cgcgtcgttc tcgccgctcg cctcgtcacc tgcgcgtgag
    67981 tggtcagcgt cgtcgtcgct atcgtgacga gcgttatcca acccagtcgc caatgccgtt
    68041 gaccgtagcg tgcgcgtctc cggaactggc ctctggcaaa gtctggatcc gctatccaat
    68101 tgtacgtccg caagatgtac aagttgaaga gcagcgcgaa caggaagaag tacaagtgca
    68161 gccgatggtg actgaggtcc ctgtcgacgc cgctgtcgaa cctgttgtta gcgcgccagt
    68221 tgttgaagaa atggctgaag tcgtagaagc ccccgtaccg gttgccgaac cgcaaccgga
    68281 agtggttgaa acgacgcatc ctgaagtgat tgccgccgcg gtaactgaac agccgcaggt
    68341 gattaccgag tctgatgttg ccgtagccca ggaagtcgca gaacacgcag aaccgatggt
    68401 tgaaccgcag gaagagacgg cagatgcaga tattgaagaa gttgccgaaa ctgctgaggt
    68461 tgtggttgct gaacctgaag ttgttgctca acctgccgcg ccagtcgtcg ctgaagtcgc
    68521 agcagaagtt gaaacggtgg ccgcggttga accagagatc accgttgagc ataaccacgc
    68581 taccgcgcca atgacgcgcg ctccggcacc ggaatatgtt ccggaggcac cgcgtcacag
    68641 tgactggcag cgccctactt ttgccttcga aggtaaaggt gccgcaggtg gtcatacggc
    68701 aacgcatcat gcctctgccg ctcctgcgcg tccgcaacct gttgagtaat aattagctca
    68761 aagtaatcaa gccctggtaa ctgccagggc ttttttattt catgtttgaa tcaacatatt
    68821 aaaattcatc aaatggcatc cttgctaacc aacaatgcaa aataggcaaa aaaaatgccc
    68881 gccccggcga tcccagagcg ggcagacaaa acatacccag tttcatgata tgttttaaag
    68941 cgagcttatt tgttgagctg gaagagcgac aatccctgca tatcggtaaa tgctttatac
    69001 gatgcctgca atgccgtttg ctgcatgatg taagatgaaa tagttgcatt ccagtccaca
    69061 tcaaccagat cgctcatctg ctgcgtttgc cctaaagcgc gatcgctacc taaggaatcc
    69121 agcgactcca gtttgttcag ctgcgtgcct aattccgcgc gaacggtaag gacattattc
    69181 agcgagtttt tcagtccacg gttggtttta tccaatgccg cagcggcgat ttctttatcc
    69241 gcttcgctat ccgcgaccgg cgttttcagg gctgcgatgg cgctatccag catggcaaaa
    69301 agattggttt cagaagcgct accgtctggt tccgctaccg cgttactggt aatactgtcg
    69361 aaaattttgt cgcccgtatg tcctatcacc atcgaacgcg aagcatcgac ctgttgctta
    69421 atactttctg ttccgccaac ataatcaccg ttagcctcac taaacggcgc agtctctgtt
    69481 ttatatgtag cgcccattac cgtcagtggt gttcgccaga ttcagcaact ggtcacgaag
    69541 cccctgaata tccgtagcca gcgaggcccg gtcatcgtca ctcaaggtgc cattgctggc
    69601 gtagacaatt ttttcctgag cattctggat agcagtggtg acctggctaa gtacactctc
    69661 ttccagtgac actttttgag tggcgaaagt acgtgccagc gtgtactggc tgttttgcgc
    69721 ctgtgcctgg gagagaacaa cagcttgtga tgcagcaatg ggatcgtctg aagggttaac
    69781 gactcgctta cccgtcgaca tctgctcgcc gtacttcatc cattctgcct gagaattggt
    69841 gatgccgcgc atgttttgct ggtacatcat ctgggtactg aaacgcattt tatctcccct
    69901 tagcgaatgt taatcagcgc atcaaaaatc gcgtttgccg tctgcagaac ctgcgcattc
    69961 gccaggtaat actgctgaaa acgttgcaga tttccgtact cctcatcgag attgacaccg
    70021 gaaatcgact gctgctgatt ggaaagctgc gtcaccacat taccttgcgt ggtgctactg
    70081 gttttcaacg tcgcggtttt attaccgata tcactcacta acgaggcata agcgtcgtta
    70141 aaggatttcg caccgcccac cgttttactg ttgctttgca gatccagcag ggcctgaccg
    70201 ttgcggttgt cgctatcacc cgcatcttct tcgctcgcca tcgcgatttt cgcttcgtcg
    70261 gtgattaata catccatgtt gacgatggcg tcacttactg gtttcagcgt gaagctgtcg
    70321 ttaacggcag gcgttcctgt aaacgtcaac tccagaccat caaatgccac tttaccgttg
    70381 gcatctggtg tcgccgtaaa agtggtattg ctggcaaggc gggtgacctg ccactgatta
    70441 ttatcgaacg agattttgta atctgtcgcc agtaccacgg aggcatcagt taccgtggca
    70501 ccgatcgcaa cgtcaccttt gtttttcgtg ttttgcagaa ccgcgggctt accgatagta
    70561 aagaaatctt caccagcatc gccgttagca tcaaatccgg ctttgtgttg gctgttgaaa
    70621 gcctcggcaa atgccagcgc cagttgtcca agcgtattac gcgtctggtc cagatcctga
    70681 gaacggaatg tcagaatgcc gcccagcgac ccggtattca gtaatttctc cgggatctca
    70741 atattgcctg ccgtcccatc aacgtaagcg acagtcgtac gagaagggtc ggcgctggaa
    70801 ggaactgccg ccagttgccg cgccgtactt ccctgaacca gtgagtaacc attggcatcg
    70861 tgatgttata agtaccgcca tcctgaacgc tgacttctac gccaacaatc tggtttaatt
    70921 cgctcaccag ttgatcgcgt tgatccagca gattgttagg tgacgcccct gcccccacgc
    70981 ctgtcaggcg cgagatttga tcgttcaggc tggcaatttg tttagcgtaa ttgttgatct
    71041 gatcaacgct ggcacctatc gcgatattga cctgtttgtc ctggtcgcgc agatattgat
    71101 cggtggtttt aaactgattc accaaccctt ctgatttccc aatcagcgcc tggcgcgccg
    71161 ccgggtcttc cgcgttactc accagcgttt gcaggctggt gaagaaatcc tgcatctgtg
    71221 ttgccagcga agaggtactg gtggagagca tattgtcgat tttcgacatc tgctcatagc
    71281 gggcggtcag gccgctactt tgcgtctgcg cagcacgtaa ctggttggta ataaacgtat
    71341 catactcacg ctgcacacca gaaacgtaga cgccattgcc aacccagccg ccagcgccca
    71401 acgtgctatt ggcctgcgcc ataatagtgg tttggcgggt atatccggca acgttatagc
    71461 tggagatatt attacttgcc gtatttaacg ccgcctgggc cgcgttcagt ccgctcatgg
    71521 cgttattgat caagctggac atggaggttc cttataagcc ttcaattaca gagtattatc
    71581 ggcagcgacc cgccggactt gagttattca gaacagatta tcaatgttca tgctgtaggt
    71641 tttgctcacc ttgtcgctta tcgatttcat ctgctggatc atgttggtga gtttacgggc
    71701 atagtgagga tcggtagcat agcccgcgtc ctgtaggacc tgcgccccct gttccgcact
    71761 cgcggcggtc gtcacggcgg cgtagcgcgg gttacgtgtt aacagcccaa cgtaatccga
    71821 caatgcttcc agatacgagc tgtagacccg aaacttcgct tttactttct tcgcttcgcc
    71881 gttttcatat tcagtcgtgg tgatttcagt gacttgccct ttccagttgc cagaggcttt
    71941 gacaccaaac aggttatagc ttggctcgcc gttttcgcgg cggatttgcc gttgccccca
    72001 accagattca agcgcagcct gagcgaggat caaatgatgt ggcacaccgc tttgctggct
    72061 tgccagttgg gcgggcagcg agagttgcgc gaggaatgct ctactgtcac ccggcagcga
    72121 atcatcgtag ttacgtggta cggccttttg caccagctgc gaaagcgtct ggttttgata
    72181 acgcaccacg gtttcgagcg ggaatttcat cggtgctgct ggcgtggact cctctggcaa
    72241 tggttgttct ggcgtcatct gtttaaccat catctctgca agccccagac ctttgcccgc
    72301 cgtcatctgt tgggcaattt gctggtcata catactggta tacagacgag tgtgctcgct
    72361 gctgaacagg ccatcttttg gtaaagcgtc gcgcatgctt ttcaacatca tctgcacgaa
    72421 catcccttcc acctgacggg ccaccggacg gatatttgcc gccggatctt cgctcgcttt
    72481 cgcctttagt tcgttgagtg attgcgcatc ccaggccgca cttgccagta gtttgctgtc
    72541 gctgatcatc agatgatttc cagttttgcc cgcagacatc ccgcactttg cattgattgc
    72601 aaaatagaca tcagatccat cggcgtagcg cccagcgcat tgagcgcacg caccacgtta
    72661 ttgaggctgg cgctggaacg tacgctttgc agcgaaccgc cgctctggcg taaatcgatc
    72721 tgcgtttgtg gagtaacaac agtctgtcca ccaccaaacg gtgtatctgg ctggctgaca
    72781 ttggcctgac gattaactgt tactgagaga tttccctgcg ctaccgcgca gctgtcgagg
    72841 gtcacttcgc gattcatcac caccgaaccg gtgcgcgagt taatcactac tttagcgtcc
    72901 tgcggggtga cattaacctg catattctgg atatcggcaa ggaaacggac ctgggaactg
    72961 ttgccacttg gtacgcgcac ctgaatagtc cgcgcatcta acgcggtggc gctgccatat
    73021 ccacgcacgc ggttgatggt gtcagcgatt tgctgcgcca tgctgaaatc ttcgtcgtta
    73081 agttgcaaat taagggtatt cccgacgcca aactggctgg gcaattcacg ttcaataacc
    73141 gcaccattgg tgatccgtcc accgttcagt tggttaacct gaacactgct accgcctgcg
    73201 gaggctcctg cgccgccaac cagaatattg ccctgcgcca gcgcatacac ctgactgtca
    73261 acgcccttaa gcggagtcat caacaacgta cctccacgca agcttttggc attacccatg
    73321 gaagaaacca ccacatcgat ggtttgcccc tgacgtccaa acggaggaag tgacgctgtc
    73381 accattaccg cagcgacgtt ttttagctgc atattggtgc ccgtcggaac ggtaattccc
    73441 agctgtgaga gcatgttatt aagcgtttgt gtggtaaacg gcgtctgggt tgtctggtca
    73501 ccagtgccat ccagccccac taccaagcca tagccaatca gtgagttttg ccttaccccc
    73561 tgaacactgg tgagatcgcg aatacgctca gcctgagccg ccgtcgtaac cagtagaaga
    73621 attaatgcag agagaaattt aatcaccaca gcctcactta cattggcgac aggttaagga
    73681 agaaacgctg caaccagccc atattttgcg cttcgttaat gtagccattg cctacgtatt
    73741 caatacgcgc atccgccacc tgagtagacg gtacggtatt gctgccgctg atagtgcgtg
    73801 gattaaccac gcccgagaag cgaataaatt cggtaccctg attaatggca atctgttttt
    73861 cacccaccac atgcaggttg ccgttgacca gtacctggtc aaccgtcacc gtcaacgtgc
    73921 cgctaaaggt attgctggca ttggccccgc cctttccgtt gaacgtgtta ccaccggagg
    73981 cttcgacatc ggcacgagcg ttaccaaaca gcccctgcaa atagcgcggc acagtatcaa
    74041 agccaaaatt agttttaccg tcacggctgg cattcgcaga ggagcttttg ctggcgctga
    74101 cgttctcctg caacacgatg gtcagcgtat cgccaatatt gcgtggtcga cgatcttcaa
    74161 acagcggttg atagccatag ttaatcggct gagcagactg gaaaatagaa ccgttggcga
    74221 cgggcgtcgg accgggaacc ggttgtgcac tggtcgcccc ctgcaccagc ggcgtggagg
    74281 gtatccaggc gcagccggtt agtgaaagca ccaacaagct ggaaatggca taagtatgcg
    74341 cagcgttttt ttgcatggct atcatcttca aaaatcagta aaccggtgaa cctgccaccg
    74401 gttatgcctt agagttgcgt cagtttttgc agcatctgat cggtggtgga caccgcttta
    74461 ctgttgattt cgtaagcgcg ttgcacctga atcatattga ccagttcttc cgccacgttg
    74521 acgttagacg tttcaacata cccttgatac agcagtcccg cgccgttcag gcccggcgtg
    74581 ctttcgttcg gtgcaccgga ggattgcgtt tcggtgtaga ggttttcgcc aatgctctcc
    74641 agtccggtat cattcataaa ggtggtgaga ttgagctgcc caacctgaac cggagctgcc
    74701 tggccttgtt gggttacgct gaccacgcca tcacgaccga tggtgatact taacgcattc
    74761 gccggaatgg tgatcgctgg ctgcacctga aaaccaccag ccgtcaccag ctgcccgttc
    74821 tgatccacct ggaaagagcc gtcacggata taggctgatg agccatctgg caacattacc
    74881 tggaaaaagc cctgcccttt aatcgcgaca tctttgctgt tattggtctg cgacagattg
    74941 ccctggctgt gtaagcgttc agtggcgacc gggcgtaccc ccgtgccgat ttgtaatccg
    75001 gagggtaagg tggtttgttc ggaagactgt gcccccggct ggcgaatggt ttgataaagc
    75061 agatcttcaa acaccgcgcg ctgacgctta aaaccgttag tactgacgtt tgccaggttg
    75121 ttggcaatga cgtccatatt ggtttgctgg gcgtcaaggc ccgttttggc gatccataat
    75181 gaactgatca tataatgtcc tgtagtggat tgctgacaag cagagcagaa gcacgcgaca
    75241 gtgtgaattt cccctcaccc taaccctctc cccaaagggg cgagggaata gtccgcgcgc
    75301 ttttattgca atgtcatcaa tcgttcaatt aactcatcga cagcagttgg ttggcacggc
    75361 ctgcgttatc atcgacgctg ctgatcacct tcatctgcat ttcaaaacgc cgcgcgctgg
    75421 caatcatgtc gctcattgcc gcaacggcat tgacgttact gccttccaga acccccgaca
    75481 tcacacgcaa ggttggatct gcctgcagta ccggccctcg cgtggcctgg gtttctgcgc
    75541 ttaaacgaaa aatgccgtcg tcaccgcgct gcacttcgct gcccgtggct ttcaccagtt
    75601 tcagacgccc tactggcgca actgtatttg ccggatcgcc cggattgagc gccgagattg
    75661 tgccatcggc agcaatcgtg atttccgccc cttccggcac agcaattggc cccgcctcgc
    75721 ctatcaccgg atgcccctga attgtcagtt gcccggtggg atcaacctga atgctgccat
    75781 tacgcgtata cccttcgctg ccgtcagcgg tctgcacggc cagccatcct gctgcaacgc
    75841 aacgtccagc gggcgagagg tgtaatccat cttgccgggc gtaatgtctg cacccggtgt
    75901 tgacgccgtt accagcgtgc gcgtgggcag tgaaagccct tccaccggca ctgcgcgcaa
    75961 ggcgtttaac tgcgcgcgaa aaccgggcgt agaggcattg gccagattac tggcggttac
    76021 cgcctgttga ttcagcgtct ggctggctgc tcccatcgca gtataaattg cgtgatccat
    76081 tgagctatcc cgtcagcgat tagcgtaagt taacccgcgt gttgaggatc tggtcctggg
    76141 ttttgatggt ctgggcgtta gacttatagt tacgctgggc aacgatcata ttgaccagtt
    76201 ctttactgag atcgacgttg gacgcttcca gcgcaccgtt ggtcagagtg ccaaagtttc
    76261 ccgtcccggc tgtccccaac agcgccacgc cagaagattg cgtcgcagac cagacgttgt
    76321 cgccttcgga tgccagacct tcgttgttgg caaagttcgc cagtacaatc tgccccagca
    76381 gttgggtttg ttcgttggaa ttgttgccga caaccgtacc gtcatcattg atttgataac
    76441 tcaccagatc gcccggtttg tagccattct gggtggttgc cacaatgttg ttagcgccgg
    76501 tattttgctg catggagttg aggaagctca gactaaacgt ggcgggttct gcaccgttaa
    76561 ttgcgccggt tgcgatatta ttcgccatcg caccatccac taatgtgcca ttagcattaa
    76621 attccagtgt tgtcgctgtc ttcgcaatgc tgtttggatc actgctatcc tgggtgtaga
    76681 cctgccagtt attatccccg gtcttcacaa aatagacgct catgtcatga gcattaccct
    76741 gactgtcgaa aacagtcacc gaaccttttt tgttatagct atccgcattg ctggcgctga
    76801 atggcgtaac agtaggaagc ggatcactgg aattcaggtt gatctgcatc gacgccgtgg
    76861 tggtagtttt cgctgccatc agggtattcg ggatcgaaat attggtcgga ttcgcccctt
    76921 gctgaatagt cggcggcgta ccggttaccg ggtaacccgt cagctgtaaa ccttgcgtat
    76981 tcaacaggtt acggttttca tccagcttaa attgtccgtt acggctgtag aacaccgaac
    77041 cgttgctgtc taccagacgg aaaaaaccgt tctggctgat agcaacgtcc agacctcgcc
    77101 cggtgttggt ggtcgtgcca tcggtaaagt cctgagtgat accggcaact tttaccccca
    77161 gtcccacttt cgaaccggca aacatatcgg caaaagaggc cgtgcctgat ttaaagccgt
    77221 aggtggcgga gttggcgata ttgttgccaa taacatcgag gttggtggcg gcagcgttta
    77281 atccgctaac cgcttgagaa aaggccatga ctgactcctg aaatgtgaag gcttaaatta
    77341 tctgccgtac ttcgtcgagg gtggtggtgc cgtaagtgcc gagatccagc gtattaccgc
    77401 tgttgccgcg gatcacaccc tgcaccagag caaactgcag cggctgggca accagttgtg
    77461 taccaccgtt actggcgcta atcgctacat tgtaagaacc gttcggcgca gtgctgccat
    77521 cagtcaacga accgtcccag gtgaaactgt gaactccggc ggtcagttca ccaatatcaa
    77581 tggtgcgcac aaccgcgcca tttttatcgg tgatggtggc cgtaactttg tctgccgcct
    77641 gttgcagctc aacaccaaac ggcgtggtcg tggtcacagc cccttcttca ctgccggttc
    77701 ccgcaagaac agttgtgccg gggatcatca cgccgtgacc gatcaggtta ctggcctgta
    77761 acgactggct gttatcaatc tgtccggaaa tagatccgag cgtggtattg agtttttcaa
    77821 tcccgctgac cgtgctgatt tgtgccaatt gcgacgtcag ctcgttgttt tccattggat
    77881 tggtcgggtc ctggtttttc agctgcgcca ccagcaaagt cagaaaactg ctttgtaaat
    77941 ctgcggcgtt gctgcccgtg agcgaactac tgctggtggt actgacgccg gtatttgtcg
    78001 gatcggtggt ggttaccgca atggacatag ctttctcctt tattgaccga gcgtaagggt
    78061 tttcagcatc atgcttttca ccgtgttgag cacttcaaca ttggcctgat agctgcgtga
    78121 cgccgacatg gtgttaacca tctccccgac gacatcaacg ttcggcattt ttatgtagcc
    78181 ctttgcatct gccagcggat tacctggttc ataaaccagt ttgtccgggg cctgactttc
    78241 ttaacatcgg caacctttac gccgcctgtc gcagcacctg gtgcagcgtt aacctggaat
    78301 accacctgtt ttgcccgata tggctgtcca tcaggaccgg tcacgctatc agcatttgcc
    78361 agattactgg ccgccacgtt caggcgctgg gactgggcag ttaacgccga cccggcgata
    78421 tcaaaaatat tcagcagtgc catccgttaa tttccgctct gtaaaacgtt catcatgcct
    78481 ttgatttgcc cgctcaacgc gctaaggctc atctggtatt gcaggctgtt atcggcaaac
    78541 tgggtgcgtt cgcgatccat atcgacggta ttaccgtcaa gcgaaggctg gtccggaata
    78601 cggtattgca gttctgcggt aggaggcgtc agcgcctgcg ccgggatgtg ctgcgttgag
    78661 gtcatcgtca gtgcaaccac actggttgca tcccgtccac gttgcatgac ttttttaagt
    78721 tcactggcaa aatcgatatc gcgcgcctga taaccagggg tatcggcatt ggcgatgttt
    78781 gctgccagca cttcctgacg ctgggcgcgc agattgagcg cctcttgttg aaaacgtaag
    78841 gcggcgtcga gcttatcgag catatctcct ccgcaggtat caaaattctg ccatcagctt
    78901 aaatgccttt acgcgcgcgt tatcggcgga ataaacgcaa aatgggtcgc tatttatgcc
    78961 gttgatggtc attgcggaca ggtacaattc acgttgtaga aatggctggg ggcgaaaatg
    79021 ctgacaataa aacgtagcgt ggcgatcatc gcgatactgt tcagtccgtt aagtacggcg
    79081 agcaatctca catcgcaatt gcacaacttt tttagcgccc aactcgcggg ggtaagtgat
    79141 gaggttcgtg tttctattcg tacagcgccc aatctactac cgccatgcga gcagccattg
    79201 ctttcgatga gcaataattc ccgcctgtgg ggcaatgtga atgtgttggc acgctgcggt
    79261 aacgacaaac gatatttaca ggttaatgtg caggccacag gaaattatgt ggttgccgcg
    79321 atgcctattg tgcggggagg aaagctggaa gctggcaatg tcaaactgaa acgtggacgc
    79381 ctggataccc tgccaccgcg tacggtgctg gatatcaatc aacttgttga tgccgttagc
    79441 ctgcgcgatc tatcacccga tcaacctatc cagttaaccc agtttcgcca ggcatggcgg
    79501 gtaaaagcgg gacaacgcgt caatgtgatc gccagcggtg atgggtttag cgccaacgca
    79561 gaaggtcagg cgctgaacaa tgcagccgtc gcacagaatg cgcgggtgcg catggtatcg
    79621 gaacaggtag tcagcggcgt tgttgatgca gatgggaata ttcttataaa cctgtaagct
    79681 gttaaagatt acccgtccct tgccgataaa taagcaacac atgataaaag cgccctcaat
    79741 gaggaataaa ccatgagtat tgatcgcact tcgcctctga agcctgtaag caccgttcaa
    79801 ccgcgcgaaa ccaccgacgc gccggtaacg aacagccggg cggcaaaaac aaccgcctcc
    79861 accagcacca gtgtgacgtt aagcgacgcg caagcaaaac tgatgcaacc cggcagcagt
    79921 gatatcaatc ttgaacgtgt cgaagcgtta aaactggcga ttcgtaacgg tgaactaaaa
    79981 atggacaccg gcaaaattgc cgatgcgctg atcaacgaag cgcagcaaga cttgcagagt
    80041 aactgatcgt atgacacgtc ttgcagagat cctcgaccag atgtccgctg tgcttaacga
    80101 tctcaaaacg gtaatggatc aagagcagca acatctctct atggggcaga tcaacggcag
    80161 ccagttgcaa tggattacag aacaaaaaag ctcactgctg gcgacgctgg attacctcga
    80221 acagttacgc aagaaagaac ccaatacagc aaatagcgtt gatattagtc aacgctggca
    80281 ggaaattact gtgaaaacgc agcaactacg ccaaatgaat caacataacg gctggttact
    80341 ggaaggacag attgagcgca atcaacaggc gctggaaatg ttgaaaccgc atcaggaacc
    80401 gacgctatat ggggcgaacg gtcagacctc aacaccccat cgcggcagta aaaagatttc
    80461 gatctgaata ttatctccgg cctgcactgc aggccggaat gcattgttac accgtccggc
    80521 gggcaaattc tttaactttg aagcccagta ccgccagtgc agcgaagtac gcggcaatcc
    80581 ccgccagcac gaccgccatt aaacgcagta agcgccaggg catggtaccc aatgaccact
    80641 ccggcatgat atgtaacata cctaaaagca cgccagacat caccagtacc gccaccacca
    80701 gacgcaacag aaacgccatc cagccgggtt gcggagtaaa gattttctgc ttacgcaact
    80761 gccagtaaag cagcgaagca ttcagacacg ccgccagacc aatagaaagt gacagcccgg
    80821 catgtttcaa cggaccaata aacgccaggt tcatcaattg cgttaaaatc agcgtaacga
    80881 tggcaatttt cactggcgtt ttaatgtcct ggcgggaata aaagccagga gccaacactt
    80941 tcactacaat caggccaatc aaacccaccg agtaggcaat taacgcccgc tgggtcatca
    81001 gcgcatcaaa cgcggtaaat ttaccgtact ggaacagcga aacggttaac ggacctgaaa
    81061 gaatgcccaa cgcaaccgca ctcggcagtg ccaacaggaa acaaagacgc aacccccagt
    81121 ccatcaaacg gttgtattca tcatgattgc cactggcaaa acttttcgac agcgacggca
    81181 gcaaaatggt gccaagcgcc acgcccagca caccggaggg aaactccatt aagcggtcgg
    81241 cgtaatacat ccaagacacc gaaccggaag caagaaacga ggcaaaaatg gtgttgatga
    81301 ttaaggagat ctggctcaca gagacgccaa ggatcgccgg tcccatctgt ttcaccacgc
    81361 gcattgctcc agcatcgtgg aagttaatgc gcggcaggac cagcatgccg atcttcttca
    81421 ggtgcggtag ctgatacacc agctgcagaa tgccgccgac cgtaacagcc cacgccagcg
    81481 ccagcaccgg tgggttaaag tacggtgcgg caaacagcgc gaaaccaatc atgctgatgt
    81541 taagcagtgt tggcgcaaac gccggaatcg agaagcggtt ccacgtattc agaatcgctc
    81601 ccaccagcga cgccagggag atcagcaaga tatagggaaa ggtaatcttt agtagctggc
    81661 tggtcaggac aaatttgtca gctgtgtcag cgaagcctgg cgcggtcacc atgatcaccc
    81721 acggtgcggc gagcatgcca gcgaccgtca caaccgccag cgcaagtgtc agcaggccag
    81781 aaacataaga gacaaagacc cgcgtggcgt cttcaccctg cttactttta tattccgcca
    81841 gaatcggtac aaatgcctgg gaaaatgccc cttcggcaaa gatacggcgt aacaagttag
    81901 gaagtttaaa agcgacgaaa aaggcgtcgg ttgccatccc tgcgccaaag attctggcga
    81961 caattgcgtc tcgtgcgaag ccaagcacac gcgaaaacat ggtcatcgag ctgacggcgg
    82021 ccagcgattt taataaattc atcggtgttc taatccggac ccacgggcaa aacgcctgca
    82081 aggcaggcgc tgaaagaggc gaatagtcta cctagattat ggtgaattgc taccgccaga
    82141 tgttacaggg tttattcact catcgcatcg cgccagatct tgtcaacgat acgttgcgcc
    82201 agcactgcct attcgccggc ggtttgcgga actgtctgat tttacacgca ttcaatgaag
    82261 tgccgcgcac agccgacaaa tccacgttgc tcaagagtgc tctgccaacc aggaatcggt
    82321 ttattcacta caccctgccc gcgctcctca cgccattcgc gcatatccgt aatgtcgatg
    82381 agcgcaccgt cagtcacggc ctgcacggtt tcacgctgac ttccggcacg gcgatgcata
    82441 caagtggtga tctgcaaagg accggccgaa aaatggtgct cggcaaacag catttcgcca
    82501 gcgtcgttag tcagtagcgt accgccatcc agagaggctt tgccgcccga caaccacagc
    82561 gcggtatcca ccacatgcag ataatcatcc agcaacgtga aataaagatc gtgtggcccg
    82621 acgctattgc tacgatgttt atccattctt agcgaggctg cggtggcgag ttgcgttttt
    82681 aactcaccgt agagtggtgc gaaacgacgg ttaaaaccga ccatcaacgt cagttttttc
    82741 cgcgccgcca gttccaccag cctttcagca tcgcgcagat tttctgccag cggtttatcg
    82801 acacagacat gtacccccgc attgagtaac gtactgacca cgtcaaagtg gctggcggtg
    82861 ctggagtgca caaaaaccgc atcacaactg gcggcaaggc tggataacga atcggcataa
    82921 ggaatgcgcc agctgtcaca aattggcagg gctttggcgc gcgtaggcga ccaggctcct
    82981 tgtaacgtcc agtcagacgc tgccgccagc accggtaacc acgctttttg cgcaatgccg
    83041 cctaatccca ctacgccgat acgtaatttt ttcactttaa tctcccagat gggccagcaa
    83101 cgaatcaaga cgttgtttca gttctgccac ttcgatttcc agggcttcga cgcgggtctg
    83161 taaatcaccg tcaaccatgt tcgacatagc cgttaccgcc ggctgatctt caacctcacc
    83221 actgaaaaga tgcatgtagc ggctttcgcg tttacccggt tcgcgggcca gacgcaccac
    83281 aaaaggacca tcttcgcgat tcgccagttg ttccagcgtc gactccactt ccgccatatc
    83341 gctgaattca tacattcgcg cggcgcggct gcgcagttca cccggcgtct gggcaccacg
    83401 caataacaac gtggtgatta acgccacttc tgcggcgctc agtttcagat cgccaaattc
    83461 tgaattacaa aaacgttgct catatttggt gacccgatta ccaaaaccgc tcactgtgcg
    83521 tagataatga cgtttgacca gattatccag ctgttcctgc acttcggatt cactcagatt
    83581 catgaccggt tcacggttcg ttttctgatt acaggccgtg actacaccat tgactgagag
    83641 cgggtattgc tccggcgtgg tcacctgctt ttccagcaag caaccaatta cgcgagcttc
    83701 cagggcagta agttgatatt tcatgagatt ctccttagcg gccgggcgtc cagtctgggg
    83761 tagttaatgc cgtcagtacg tgatcgcgcc attgtccatc aatcaacaga tagtctttcg
    83821 catagccttc tttttcaaaa cccagtcgcg ccagtaaatc accgctgcgt ttattgtgcg
    83881 gcatataatt agccataatg cgatgaatat gttgggtgcg ctgcatataa cgaatggctg
    83941 cggtcagggc ttcaaacatg agtcctttgc cctgccattt ttgcccaatc gaataaccga
    84001 gatagcaggc atgaaaagag ccgcgaacaa cattggaaaa attggcaacg ccaataatct
    84061 ctttttcgtc cgggtcgaat aagccaaagt agaaagctga accttgttta tgaaattcgt
    84121 taatcatccc tagcctggcc tgccagcctg atggataaca gtggctttcg tcgcgcactg
    84181 gctcccaggg cttgaggaaa tggcgattct ctgcgtaata atccgccaga cgccaggcat
    84241 cacgatcatg caccagacgc acgaccagtc ggtctgtggt taagcgcact tttggcacgt
    84301 tactgcgata gccaaacatt ctatacctac tccttcccgt aacgtcttta atacgctgta
    84361 agtattacta tacctgcgca accagaccct gtgaaaagtc caaaatgcca ttttctgcat
    84421 tattcacata tttgataaaa actctctatc aaagcgacgt tttgataaaa aaatactgtc
    84481 cccgaagtga tgggaaaagt cttagcggca atgcagaata gaaagtgctc atcttttatt
    84541 tcccgggagg ggaaatgtcc cgcgtatcgc aggcgaggaa cctgggtaaa tatttcctgc
    84601 tcatcgataa tatgctggtc gtgctggggt tctttgttgt cttcccgctg atctctatcc
    84661 gcttcgttga tcaaatgggc tgggccgccg tcatggtcgg tattgctctc ggtctacgcc
    84721 agtttattca gcaaggtctg ggtattttcg gcggcgcaat tgccgaccgc tttggtgcca
    84781 aaccgatgat tgttaccggt atgctgatgc gcgccgccgg attcgccaca atgggtatcg
    84841 cccacgaacc gtggctattg tggttttcat gcctgctctc gggactcggc ggcacgttgt
    84901 ttgatccgcc gcgttcggcg ctggtggtga aactaatccg tccacaacaa cgtggtcgtt
    84961 ttttctcgct gttgatgatg caggacagtg ccagtgcggt cattggcgca ttgttgggga
    85021 gctggctgtt gcaatacgac tttcgcctgg tctgcgccac aggggccgtt ttgtttgtgc
    85081 tatgtgcggc gttcaatgcg tggttgttac cggcatggaa actctccacc gtacgcacgc
    85141 ccgttcgcga aggcatgacc cgcgtgatgc gtgacaagcg ttttgtcacc tatgtcctga
    85201 cgctggcggg ttactacatg ctggctgtac aagtgatgct gatgctgcca attatggtca
    85261 acgacgtggc tggcgcgccc tctgccgtta aatggatgta tgccattgaa gcgtgtctgt
    85321 cgttaacgtt gctctaccct atcgcccgct ggagtgaaaa gcattttcgt ctggaacacc
    85381 ggttgatggc tgggctgttg ataatgtcat taagcatgat gccggtgggc atggtcagcg
    85441 gcctacaaca acttttcacc ctgatttgtc tgttttatat cgggtcgatc attgccgagc
    85501 ctgcgcgtga aaccttaagt gcttcgctgg cggacgcaag agctcgcggc agctatatgg
    85561 ggtttagccg tctgggtctg gcgattggcg gcgctattgg ttatatcggt ggtggctggc
    85621 tgtttgacct gggcaaatcg gcgcaccagc cagagcttcc gtggatgatg ctgggaatta
    85681 ttggcatctt cactttcctt gcgctgggtt ggcagtttag tcagaaacgc gccgcgcgtc
    85741 gtttgctgga acgcgacgcc tgatttgata ctcccccctg actcctccat actgaaagtg
    85801 aactgacagc caaatggagg agtcacgtga agctatacat ttacgatcac tgcccttact
    85861 gcctcaaagc ccgcatgatt ttcggcctga aaaatatccc cgtcgaatta catgttctgc
    85921 tcaacgacga cgcagaaaca cccacccgga tggtcggtca aaaacaggtt cccattctgc
    85981 aaaaagatga cagccgctat atgccagaaa gcatggatat cgttcactat gtcgataaac
    86041 tcgacggcaa accgttactg accggcaaac gttcccctgc cattgaagag tggctgcgca
    86101 aggtcaatgg ctacgtcaac aaactgctgt tgccgcgttt tgccaaatcg gcatttgatg
    86161 agttttctac tcccgccgcg cgcaaatatt tcgtcgacaa gaaagaggcc agcgcgggta
    86221 attttgccga cctgctggcc cactctgacg gtctgattaa gaatatcagc gatgatttgc
    86281 gtgcgctgga caaactgatc gtcaaaccga acgccgtgaa tggcgaactt tcggaagatg
    86341 atattcagct atttccgcta ctgcgtaatc tgacgctggt agccggaatc aactggccaa
    86401 gccgcgttgc taattaccgc gataatatgg cgaaacagac acaaatcaat ttgttatcat
    86461 caatggcgat ttaatcctga cccgccgcgg cagcctgtat tcgcgggttg ccgccgtttt
    86521 cagttttttc tgctggcgag tttatcgctg gtgacacatg ctgcgttgtg tcaggatatt
    86581 gacgcagatt tcatgtcgag gactaccatg aacaagtttt tatttgccgc tgcattgatc
    86641 gtcagtggcc tgctcgttgg ctgtaatcaa ctcacccaat acaccatcac cgaacaagaa
    86701 attaaccagt cgcttgcgaa acataataat ttctcaaaag atatcggttt acccggcgtg
    86761 gctgacgccc atattgttct gacaaacttg accagccaaa ttggtcgcga agagccgaat
    86821 aaggttaccc taaccggaga cgccaatctg gacatgaact ccctgttcgg tagtcagaaa
    86881 gcgaccatga aactgaagct gaaagcgctg cccgtgtttg ataaagaaaa aggtgcgatc
    86941 ttcctgaaag agatggaagt ggtcgatgcg acggtacaac cggaaaaaat gcaaacggtg
    87001 atgcaaacgt tgcttcccta tttgaaccag gcattacgca attactttaa ccagcaacct
    87061 gcttacgtcc tgcgcgaaga tggcagtcag ggcgaagcaa tggcgaaaaa actggcgaaa
    87121 ggcattgaag tgaagccagg cgaaattgtc attccgttta cggattaatg acgagggcgc
    87181 attcgcgccc tttatttttc gtgcaaagga aaacgtttcc gcttatcctt tgtgtccggc
    87241 aaaaacatcc cttcagccgg agcatagaga ttaatgactg caccatccca ggtattaaag
    87301 atccgccgcc cagacgactg gcaccttcac ctccgcgatg gcgacatgtt aaaaactgtc
    87361 gtgccgtata ccagcgaaat ttatggacgg gctatcgtaa tgcccaatct ggctccgccc
    87421 gtgaccacag ttgaggctgc cgtggcgtat cgccaacgta ttcttgacgc cgtacctgcc
    87481 gggcacgatt tcaccccatt gatgacctgt tatttaacag attcgctgga tcctaatgag
    87541 ctggagcgcg gatttaatga agccgtgttc accgctgcaa aactttaccc ggcaaacgca
    87601 accactaact ccagccacgg cgtgacgtca attgacgcaa tcatgccggt acttgagcgc
    87661 atggaaaaaa tcggtatgcc gctactggtg catggtgaag tgacacatgc agatatcgac
    87721 atttttgatc gtgaagcgcg ctttatagaa agcgtgatgg aacctctgcg ccagcgcctg
    87781 actgcgctga aagtcgtttt tgagcacatc accaccaaag atgctgccga ctatgtccgt
    87841 gacggaaatg aacggctggc tgccaccatc actccgcagc atctgatgtt taaccgcaac
    87901 catatgctgg ttggtggcgt gcgtccgcac ctgtattgtt tacccatcct caaacgcaat
    87961 attcaccaac aggcattgcg tgaactggtc gccagcggtt ttaatcgagt attcctcggt
    88021 acggattctg cgccacatgc acgtcatcgc aaagagagca gttgcggctg cgcgggctgc
    88081 ttcaacgccc caaccgcgct gggcagttac gctaccgtct ttgaagagat gaatgctctg
    88141 cagcactttg aagcattctg ttctgtaaac ggcccgcagt tctatggctt gccggtcaac
    88201 gacacattca tcgaactggt acgtgaagag caacaggttg ctgaaagcat cgcactgact
    88261 gatgacaccc tggtgccatt cctcgccggg gaaacggtac gctggtccgt taaacaataa
    88321 aaatcgtagc ttcctgttgt cattaggtta ttttacctgt ataaataacc agtatattca
    88381 acagggggct attatgcgaa ttgaagtcac catagcgaaa acttctccat tgccagctgg
//
LOCUS       DADLTY010000002        67671 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.002, whole genome shotgun sequence.
ACCESSION   DADLTY010000002 DADLTY010000000
VERSION     DADLTY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.002"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..228
                     /locus_tag="JN334_000096"
     CDS             <1..228
                     /locus_tag="JN334_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436376.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            623..1262
                     /locus_tag="JN334_000097"
                     /pseudo
     CDS             623..1262
                     /locus_tag="JN334_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1365..1688
                     /locus_tag="JN334_000098"
     CDS             1365..1688
                     /locus_tag="JN334_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HAY7436377.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="JN334_000099"
     CDS             complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="JN334_000099"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAY7436378.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="JN334_000100"
     CDS             complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="JN334_000100"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAY7436379.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="JN334_000101"
     CDS             complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="JN334_000101"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAY7436380.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="JN334_000102"
     CDS             complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="JN334_000102"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAY7436381.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="JN334_000103"
     CDS             complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="JN334_000103"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAY7436382.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="JN334_000104"
     CDS             complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="JN334_000104"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAY7436383.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="JN334_000105"
     CDS             complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="JN334_000105"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAY7436384.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="JN334_000106"
     CDS             complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="JN334_000106"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HAY7436385.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="JN334_000107"
     CDS             complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="JN334_000107"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAY7436386.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQQFSNTHLINGIQPNLLKERW"
     gene            complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="JN334_000108"
     CDS             complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="JN334_000108"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAY7436387.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="JN334_000109"
     CDS             complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="JN334_000109"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HAY7436388.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15658..16320)
                     /locus_tag="JN334_000110"
     CDS             complement(15658..16320)
                     /locus_tag="JN334_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAY7436389.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="JN334_000111"
     CDS             complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="JN334_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HAY7436390.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="JN334_000112"
     CDS             complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="JN334_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HAY7436391.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19267..20484
                     /locus_tag="JN334_000113"
     CDS             19267..20484
                     /locus_tag="JN334_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAY7436392.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20568..21167
                     /gene="yfbR"
                     /locus_tag="JN334_000114"
     CDS             20568..21167
                     /gene="yfbR"
                     /locus_tag="JN334_000114"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY7436393.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21226..23058)
                     /locus_tag="JN334_000115"
     CDS             complement(21226..23058)
                     /locus_tag="JN334_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAY7436394.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="JN334_000116"
     CDS             complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="JN334_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HAY7436395.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23806..24300)
                     /locus_tag="JN334_000117"
     CDS             complement(23806..24300)
                     /locus_tag="JN334_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAY7436396.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24383..24838)
                     /locus_tag="JN334_000118"
     CDS             complement(24383..24838)
                     /locus_tag="JN334_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HAY7436397.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25176..26378
                     /gene="ackA"
                     /locus_tag="JN334_000119"
     CDS             25176..26378
                     /gene="ackA"
                     /locus_tag="JN334_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAY7436398.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26453..28597
                     /gene="pta"
                     /locus_tag="JN334_000120"
     CDS             26453..28597
                     /gene="pta"
                     /locus_tag="JN334_000120"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY7436399.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28787..30307
                     /gene="yfcC"
                     /locus_tag="JN334_000121"
     CDS             28787..30307
                     /gene="yfcC"
                     /locus_tag="JN334_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAY7436400.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="JN334_000122"
     CDS             complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="JN334_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HAY7436401.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="JN334_000123"
     CDS             complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="JN334_000123"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HAY7436402.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="JN334_000124"
     CDS             complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="JN334_000124"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HAY7436403.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32327..32974
                     /gene="yfcG"
                     /locus_tag="JN334_000125"
     CDS             32327..32974
                     /gene="yfcG"
                     /locus_tag="JN334_000125"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HAY7436404.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33031..33393
                     /gene="folX"
                     /locus_tag="JN334_000126"
     CDS             33031..33393
                     /gene="folX"
                     /locus_tag="JN334_000126"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HAY7436405.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33414..34307
                     /locus_tag="JN334_000127"
     CDS             33414..34307
                     /locus_tag="JN334_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAY7436406.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34355..35245)
                     /locus_tag="JN334_000128"
     CDS             complement(34355..35245)
                     /locus_tag="JN334_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY7436407.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="JN334_000129"
     CDS             complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="JN334_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HAY7436408.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36223..36939)
                     /locus_tag="JN334_000130"
     CDS             complement(36223..36939)
                     /locus_tag="JN334_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7436409.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36936..37622)
                     /locus_tag="JN334_000131"
     CDS             complement(36936..37622)
                     /locus_tag="JN334_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708190.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY7436410.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="JN334_000132"
     CDS             complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="JN334_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HAY7436411.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38715..39497)
                     /gene="argT"
                     /locus_tag="JN334_000133"
     CDS             complement(38715..39497)
                     /gene="argT"
                     /locus_tag="JN334_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HAY7436412.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39763..40332)
                     /locus_tag="JN334_000134"
     CDS             complement(39763..40332)
                     /locus_tag="JN334_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY7436413.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40427..41944)
                     /gene="purF"
                     /locus_tag="JN334_000135"
     CDS             complement(40427..41944)
                     /gene="purF"
                     /locus_tag="JN334_000135"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAY7436414.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="JN334_000136"
     CDS             complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="JN334_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HAY7436415.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="JN334_000137"
     CDS             complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="JN334_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAY7436416.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43380..44648)
                     /gene="folC"
                     /locus_tag="JN334_000138"
     CDS             complement(43380..44648)
                     /gene="folC"
                     /locus_tag="JN334_000138"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAY7436417.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44718..45632)
                     /gene="accD"
                     /locus_tag="JN334_000139"
     CDS             complement(44718..45632)
                     /gene="accD"
                     /locus_tag="JN334_000139"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HAY7436418.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45788..46447)
                     /locus_tag="JN334_000140"
     CDS             complement(45788..46447)
                     /locus_tag="JN334_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7436419.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46530..47342)
                     /gene="truA"
                     /locus_tag="JN334_000141"
     CDS             complement(46530..47342)
                     /gene="truA"
                     /locus_tag="JN334_000141"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAY7436420.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47342..48355)
                     /locus_tag="JN334_000142"
     CDS             complement(47342..48355)
                     /locus_tag="JN334_000142"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY7436421.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="JN334_000143"
     CDS             complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="JN334_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HAY7436422.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49656..50651
                     /locus_tag="JN334_000144"
     CDS             49656..50651
                     /locus_tag="JN334_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HAY7436423.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50648..51826)
                     /locus_tag="JN334_000145"
     CDS             complement(50648..51826)
                     /locus_tag="JN334_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7436424.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="JN334_000146"
     CDS             complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="JN334_000146"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HAY7436425.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53481..55487
                     /gene="mnmC"
                     /locus_tag="JN334_000147"
     CDS             53481..55487
                     /gene="mnmC"
                     /locus_tag="JN334_000147"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAY7436426.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55608..55886)
                     /locus_tag="JN334_000148"
     CDS             complement(55608..55886)
                     /locus_tag="JN334_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HAY7436427.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55920..56468)
                     /locus_tag="JN334_000149"
     CDS             complement(55920..56468)
                     /locus_tag="JN334_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAY7436428.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56468..57277)
                     /locus_tag="JN334_000150"
     CDS             complement(56468..57277)
                     /locus_tag="JN334_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAY7436429.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="JN334_000151"
     CDS             complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="JN334_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HAY7436430.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="JN334_000152"
     CDS             complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="JN334_000152"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAY7436431.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="JN334_000153"
     CDS             complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="JN334_000153"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY7436432.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60323..60874
                     /gene="smrB"
                     /locus_tag="JN334_000154"
     CDS             60323..60874
                     /gene="smrB"
                     /locus_tag="JN334_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HAY7436433.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60978..61868)
                     /locus_tag="JN334_000155"
     CDS             complement(60978..61868)
                     /locus_tag="JN334_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HAY7436434.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61855..62379)
                     /locus_tag="JN334_000156"
     CDS             complement(61855..62379)
                     /locus_tag="JN334_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7436435.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62376..62846)
                     /locus_tag="JN334_000157"
     CDS             complement(62376..62846)
                     /locus_tag="JN334_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7436436.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62843..63391)
                     /locus_tag="JN334_000158"
     CDS             complement(62843..63391)
                     /locus_tag="JN334_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7436437.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63396..64118)
                     /locus_tag="JN334_000159"
                     /pseudo
     CDS             complement(63396..64118)
                     /locus_tag="JN334_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64138..66778)
                     /gene="yfcU"
                     /locus_tag="JN334_000160"
                     /pseudo
     CDS             complement(64138..66778)
                     /gene="yfcU"
                     /locus_tag="JN334_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66860..67423)
                     /locus_tag="JN334_000161"
     CDS             complement(66860..67423)
                     /locus_tag="JN334_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7436438.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16924 a        18281 c        16783 g        15683 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaataac
      601 gccttttatt gataatttac atatgaaaaa attttccttt tgttatatta tttctgtttt
      661 cactccctct gattattttt accaaccctg ggtcactgca ttatcgccaa cgccccgaca
      721 cgtaagtcct gagcaattag aaaaaacagt tcgttatctt acacaaactg tgcatccacg
      781 taatgccggc aatattgata atctgaatag gtccgcagag tatataaaag agttctttgt
      841 cagtagcggt gccagagtta ccgcgcaggg cgtccccgat tggctattac gactctgcgc
      901 ctggaagcca gaattaccct tacccggcaa tgtcctggct ttatcccgat cggggagact
      961 ttattgctgt ggtcggcaga atgcaggata tcaacgccgt tcgtcaggta aaagcggcat
     1021 tgttgtcatc ccgggattta tctgtttatt ctatgaatgc cccagggttt attcccggta
     1081 ttgatttttc tgatcacctc aattattggc aacacgatat tcccgccgta atgattactg
     1141 acaccgcttt ttatcgtaat aaacaatacc acttgcccgg tgataccgca gacagattga
     1201 attatcagaa aatggctcag gtggtagatg gtgttataac cttgttatac aacagtaaat
     1261 aataaaaatt tctcgaggcg ggtccagaaa cagcgacatg tacattttat tttccctcgt
     1321 ctgttttaag atgcgaaaca taaaattcaa tggaggaaaa ataaatggga atgattggct
     1381 attttgcaga aattgactca gaaaagatta accaactgct tgagtctact gaaaaacctt
     1441 tgatggataa tatacatgac acgctttcag gattacgccg actggaatca ctggatcgaa
     1501 gtgagctgcg taaacagttt tccattaagc gactcaatga aatggagatt tatccgggag
     1561 tgaccttttc agaagagtta gaggggcaac tctttgcctc gatcatgctt gatatggaaa
     1621 agctgattag cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg
     1681 ttgggtgatg gtgtcagagc gccaaaaaaa accgccgaac atgtcggcgg ctttctgact
     1741 tacaaactaa cagtttacat cagcggcatt gctaaccgca caatactaat cagcggttgt
     1801 ggccatacac ccagcaccag taccaacagt gcagaaatca gcaccacgat accgcccgcg
     1861 ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt ccggtgcgtg caggtacagg
     1921 ctcaccgcca cgcgcaggta gtagtagagg ccgattgccg aaccgacaac cacggcaccc
     1981 accagccacc acaagtgtgc ctggacaccg accgccagca cgtagaactt accgataaag
     2041 cccagcgtca tcgggatacc ggccagagac agcatcatca ccgtcatcac tgccgcgagg
     2101 atcggacgat gccagaacag accgcggtaa gagaacaggg aatcagcatc cgggccacga
     2161 tacgggctgg acatcaggct gaccacgccg aacgcgccga ggctgctgaa cagataaccg
     2221 gccaggtaaa cccctaccgc ttccatcgac atctcgccgg tttgcagcgc aatcagcgcc
     2281 accagcagat agccgaggtg agagatagat gagtaaccga gcagacgttt gatgttggtc
     2341 tggctcaacg ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagtact
     2401 acgcgaatcg cttcgctgtc acccaccggt gcgtagagga acagacgcat caccacaccg
     2461 aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg
     2521 tatacgtctg gcgtccacag gtggaacggc accagagaga gtttgaagcc gaggccaaca
     2581 atcatcatgc cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccgaggttt
     2641 ttacccagcg cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccgaac
     2701 agcaggaaag aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagcgaa
     2761 cgtttctggc ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcaata
     2821 ccgaggaaca gagacgccag atggtttgca ttcgccagca ggatcccgcc cagcgcggca
     2881 attaacacca acaggtagaa ctcatccttg ttgtcgttat agccttcaag ccacgggtag
     2941 gcgaaagtac aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa
     3001 ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcccg cctggccaac aaaccagagc
     3061 gaaaccagcg ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt
     3121 cgccacgcaa tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc
     3181 agtgcgatca ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg
     3241 gaattaacaa accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc
     3301 tgcggataga agcccagcag taccagcagc accaccagca acaggatcat aaacagctca
     3361 cgcagcgaca tccctggcag ttcctggctg gcaatctggc ttttcgcttt accgaagtaa
     3421 gcgcgatgca acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata
     3481 acggtaatca ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag
     3541 ttaccggtgc caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc
     3601 catttcatct tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca
     3661 taaagctgac cgcagagaat aaacagaccc gccgccgaca agccgtgcgc aatcatctgg
     3721 attaccgcgc cctggtaggc caactggctg ccggtgtaga ttgcaatcag cacgaagccc
     3781 atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat cggtctgagc gaaggtcatc
     3841 cacgcaccgt agaagatgcc gataacaccc agccacatag cgattggcgc gaactctgcc
     3901 gacgcgttcg ggaacagcgg cagggagaaa cgcaacaaac cgtaagcggc ggttttcagc
     3961 aagatccccg cgaggtccac ggaaccggcg gtcggagcct gggagtgcgc atccggcagc
     4021 cagccatgca gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatc
     4081 aacagatatt ccacaccact ggacattggc gtattcagca gctcttcata gttgaaggtc
     4141 cagacgccgg tcgcattgta gtgaacaaaa accagcgcca ggatggcgat caacatcacc
     4201 agaccactcg cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg
     4261 tcagaggctt tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc
     4321 cagaagaaga agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg
     4381 atccacatca ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacat
     4441 agtaccgcca gcacaccgag cagaccggtc agcacgacca tcagcagcga cagaccgtca
     4501 atggcgagat gaatagagat accaaaacgc gggatccacg gcatgtcgaa ttcagactgc
     4561 cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc
     4621 gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc agcgtggcac cttgacgcca
     4681 aagcgttcgg tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt
     4741 agtaacatgg cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat
     4801 tctcacgaca gaaaattctc ttcggactgg gggtagtgtc ggatgcgccg cgaaggcagc
     4861 ttatccgacc tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag
     4921 taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg
     4981 cagataaccg ttctcgctta acagcagacc tttacctgca aagcgggaaa gaaccgccgg
     5041 gatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg
     5101 cttgacgaac actttgtcgt acagccagtc aaagccccaa gcgttgtacc accaggtact
     5161 cagcaggcgg cccggcgcac tgttggcgat ggaggtcacc agagaacgtt tacccagcca
     5221 cagccaggct gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa tttccagggt
     5281 caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac
     5341 aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt
     5401 tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat
     5461 acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat
     5521 atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac
     5581 cagcggcagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat
     5641 agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg ccagaatgac
     5701 ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat
     5761 cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt
     5821 agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag
     5881 cagcgtaacc gccccgacaa tacccaccag atgcagaact tccggcgtca tcaggaacag
     5941 gccgtgggta cgggcgatca ggtagacacc cgcggttacc atcgttgcgg cgtggatcag
     6001 cgcggagaca ggcgtcgggc ccgccatcgc gtcagcaagc catgtctgca acggcaactg
     6061 cgcagattta ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt
     6121 gccgtcagca aagtgcgctg gtgccagttc caccatatcg cggaagttca gggtgcccag
     6181 ttcgttgtaa agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac
     6241 gaacgctttc attgctgctg cgccattctt cggatcggtg taatagaaac cgatcagcag
     6301 ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt tatcggcgag
     6361 aaccagaact accatgctgg cgatgaacag gttggtgtag gcgaagaagc gagagtagcc
     6421 ctcttcaccg cgcatgtacc aggaggcgta catgtgaata aggaaaccca caccagtgac
     6481 caccgagagc atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc
     6541 gcctaccgac atccacgtcc acagcggctg gctgtatgcc tgctcgccgt tagcgaagaa
     6601 atcaacgccg ataaaggcgg ttaccagcgc cgccaggccc acagagccaa cgccgacgat
     6661 cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc agcaggacga agccaatcaa
     6721 tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg
     6781 ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct
     6841 gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg accccagtag
     6901 cttccggcga ccacaaaggc cagcgcggag gcgttaatca tgatttccag accaatcagc
     6961 ataaacagca gattgcgacg gataaccaga ccggttaagc caagaacgaa taagattgcc
     7021 gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc
     7081 tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg
     7141 ccacaaccag acccgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc
     7201 cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata ccctgatcgt
     7261 taacaccgag gatggcgtaa acaatcacca ccagcatgat ggccgacaaa attgccgggc
     7321 caatccacac ctgcggtttc agccactggc gttcctgttc gatttctgaa ccgcccaggt
     7381 tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt
     7441 ccagcgcacc ggcgaagtaa gcgcctagtg agaagaacac cccggaaatc gccagcagcg
     7501 aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg
     7561 caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg
     7621 taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc
     7681 gtcgattgcc atacctgcca tccggtagaa gttatattcc gggtatttgc ccggaccgga
     7741 gatcagcaga tcctctttct cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa
     7801 atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacagac cacagaaaat
     7861 gcagcgtgag aagttaatgc ggaaaaattc cggataccag cgaccgtctt tggtttctgc
     7921 tttttgcagc gagatacagc cgaccgggca ggctaccgca cagaggttac aggctacgca
     7981 acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag
     8041 atagaccggc tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat
     8101 catccagata ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt
     8161 cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc
     8221 agcgtcagcg gcaggcagat tttccagccg aaggacatta cccggtcata acgcggacgc
     8281 ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac
     8341 cagatgaatg gcggtaacaa cgggccttgc cagccaccga agaacagcgt caccatcaat
     8401 gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg
     8461 gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg
     8521 tgacggtgac acaccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg
     8581 atgacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc
     8641 tgcgccacca cgcccatcag ggaaagcccg aggaacactt cgtagctcag ggtctgcgca
     8701 gaagcacgca tcgcacccag cagcgagtat ttgttgttac ttgaccagcc cgcaaacagc
     8761 accgcgtaaa ccgccagacc tgccatcatc aggaagaaca aaatcccgat gttcaggtcg
     8821 gcaaccaccc aacccggact gactggcaca attgcaaagg ccagcagcag cgaggtaaag
     8881 gcaatcatcg gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct
     8941 ttaaagaaca ttttgatcat gtccgcaacc agctggagcg aaccgcccca gccaacacgg
     9001 ttaggtccat aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat
     9061 gccccacagg tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc
     9121 tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg
     9181 cgccagccag caccggagca atgccggaca tacccatcgg caagcccacc tgccctgccg
     9241 tcagtccttc ggcgatttca accggcagcg tgaccgtgtt gccatcgtaa ctaaaggaga
     9301 cgcgtgtacc tgcgttcaca cccaacttcg cggcatccgc tgggttgagt ttgatgtacg
     9361 gctgcggcat acggctctgg aagaccggag cacgctgtga caattcatcg ctgccaaaca
     9421 ggtggtagta cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc
     9481 tggtgaagta atccagaccg ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa
     9541 agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc
     9601 ccggcgccca ggcaaacggc acttgcgaac ggtgcgcggt cggctggttg ttaccttcca
     9661 tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat
     9721 tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac
     9781 gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc cgccagttct gggattttcg
     9841 ccacaacagc gtcaattaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaagg
     9901 tgctgtgcag cgagtgcagc cagcgccagc tttccagcat gacagttttg ctgtcgtaat
     9961 aagccggatc gtaaacctgg aagaaacgtt gggcgcgacc ttcattatta attaccgtac
    10021 cgtcgctttc agcaaagctg gcagaggaaa gcaccagatg ggcgttttcc atgatcgctg
    10081 tgcgttgatg atcaaccacc atcaccagcg gcgctttagc cagcgcagca ttcacgcggg
    10141 tagcagaagc gtaacgatgc agatcgtttt ccaacaccac caccgcgtcg gcgcgtccgg
    10201 tttccagttc ggttaacgct tcttcaagcg aaccgccacc cataatgccc agccccatgc
    10261 tgttgacgga acgggcaatc atggtgatac caacgtcagc gccgcgacct ttcagggctt
    10321 tcgcgacgtt agccgcagcc tgaatcacct ctatgctacc ggcgttcgtc ccggagataa
    10381 tcaacggttt cttcgcacct gccagtgcct gcacgatgac gtcgattttg ttttgcagct
    10441 caggttcgat accgtcaacc gctggtgcgg agttatccag cgcatgggcg atggcaaaac
    10501 ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac
    10561 gagtgtcatc aacgttggta acaaacagcg gatgcttcgc acgttgaccg atgttgagga
    10621 ttgccgcaat ctgccagtca gccactttct gtgctgcagc catttcgcgc gctttacctt
    10681 tcaccgcctg acgcactgcc agtgcgacgc gcgcgccggt ctgggtaacg tcttcgccca
    10741 gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt
    10801 cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt
    10861 agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg
    10921 gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg
    10981 cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac
    11041 gatccttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt
    11101 ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt tcaccggggc
    11161 tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt
    11221 tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga
    11281 ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt
    11341 tgtcgtgcgc gccataaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca
    11401 cacagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaatggc cccaaatcct
    11461 gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacgg
    11521 tcatatcctg aagatggcag ttaccgcctt cttcacatac cggacagtcg tgcgggtggt
    11581 tggtcattaa ccactcgacc acactttcac ggaactgttt cgcttcttcg tcgtcaatgg
    11641 aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt
    11701 cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgacg cttcccagcg
    11761 ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt
    11821 tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc
    11881 ttccagtttt ctcggtcagg gaccgagcgt taatcgaaat tcggttacca gcgctctttc
    11941 agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaactgctg tttgattccc
    12001 gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac tgcaccaggt
    12061 gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg
    12121 atatcgcccg gctggccttc gccacgctcc agcgcacgca gaattttcac gctccacggc
    12181 agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa ctcttccagg
    12241 ttacgcacca gcgacaccat gttgatctca tggtcaaccg ccatcgccag cgccgtaccc
    12301 agacggctgc ccgctttacc gatactttcg aattccatcg gcagatcaag gtgcgcttcg
    12361 gtcaggaagt cagtccccgc gccgcctggc tgccaggctt taaatttcag accatcacgc
    12421 ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac
    12481 agtcccggat ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt
    12541 ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg
    12601 gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc cggaggttgc cgggaagggg
    12661 ggtttcgagc gtgggttagc acgacgccct tccagggagt tgattaacgc tgtttcttcc
    12721 ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa acctgttccc
    12781 ataatgtttt tgccaagcaa gcccgcttca gtggcttcgg caatggcgcg gcgcagattt
    12841 actgccgctt cgatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca
    12901 aacgcggaga tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg
    12961 tctttatagg tgcccggctc catttcatcg gcgttacaca gcaggtaacg gatgttcatg
    13021 gattcgtctt tcggcatcag gctccacttc aggccagtcg aaaagcccgc gccgccgcgc
    13081 cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc
    13141 gccttacgcg ccccttcgta accgtttttg ctgcggtatt cgtccagcca cactggctgt
    13201 ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc
    13261 atttataccg ctccagcagt tcagggatcg cttccggggt cagatgcgcg tgagtgtcct
    13321 catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt ggcagcagcg
    13381 taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt cagctttttc tcgagcgccg
    13441 cctgaatacc ctgatacccg ttgatatgac agaccacgct gtcgcaataa cggatcacat
    13501 ggcgaccaac cggctggcgg aagatctgac tgtagaacgt tgccacacct tcgacgtcgc
    13561 ttgccggaat acccagcaca tcggcgatcg cgtggatcgc accatccggc acccagccac
    13621 gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt
    13681 gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg
    13741 gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc
    13801 cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa
    13861 atgcgcataa ctcggcgtgc ggatacgagt gcgataactc atggtgctac cgtcgctggt
    13921 caggtagtaa ctgttgatcc ccttggtcgc ctcaatcatc tggaaagatt cattggcagg
    13981 catcaccggc ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag
    14041 cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg
    14101 catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttcag
    14161 cattacgcgg gtgtagcagt cagaaacacc accaccaacc gggatttcaa agtcgaagtt
    14221 ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag
    14281 gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg
    14341 ggaacgacct ttcagaatgg tattttgcag cgccgctttc tcgtaagacg ccagacgttt
    14401 cggcatccag tcgaggaact cacgcagcag acgatcccag ccgcgcggca ggtcgtgcgc
    14461 tacgccgcca atacggaacc acgccgggtg catacggaaa cccgtgatcg cttccaccag
    14521 atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc
    14581 ctgaataaag gtcgagatgt acagcaggtg gctgttgata cggaacagtt cggagagcat
    14641 aacgcgaatg acgttaacgc gatccggcac ggtgatcccg gccagtttct ctaccgccag
    14701 cacgtaaggc atttcgttaa cgcagccgcc gaggtattca atacggtcgg tatacggaat
    14761 gtagctgtgc caggactggc gctcgcccat tttctccgca ccacggtggt ggtagccgat
    14821 gtctggtacg cagtcgacaa tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc
    14881 gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcat tttcggtgcc
    14941 gcgcttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt
    15001 ggctttggtc agctcaaacg gcgagaattc ggtagcgcgt gccgggtaat ctttacgcag
    15061 cgggtgacct ttccaggttt gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa
    15121 agtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt
    15181 ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg
    15241 gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc
    15301 gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt
    15361 cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac
    15421 gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag
    15481 ttcgccaatt accggatcat caagatgatc gcgggtctgc caggcgggtt cttgcgcggt
    15541 taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc
    15601 ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta
    15661 aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct tgcgttcgcg
    15721 ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg
    15781 acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg
    15841 cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac
    15901 ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt
    15961 tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt
    16021 tcctgcaacc accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa
    16081 acgcgccacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag
    16141 accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt
    16201 gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt
    16261 ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat
    16321 cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg
    16381 ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag
    16441 acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc
    16501 gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa
    16561 catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat
    16621 accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc
    16681 gcctaccagc atcaggcaac acaggccaat ggcaacgata agaaagatag cgaatgccca
    16741 gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc
    16801 gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa
    16861 aaaaacctta caataactgt agaaattctt ttatacagct aattgatgtg gtcttttact
    16921 cctttctata accttttgtc aactttaaca aaagcttctt cacattagtt tacataatat
    16981 caacaccatt agcatttaat gccctttcac cccagatcct tgacgactcc aggataatta
    17041 gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct attttgacag
    17101 gaatttgtgt cgattcctcc ccccaaaaga gagtattttc ttgatctgtg acacgctttt
    17161 gttattccat aacaaaaacg cagcaacaaa tttacgtatt ttttaacatc attgtagcag
    17221 gtgatttttt tcaggcgatt atttgtgcat tcgggacgtg aatctctggt gattgaaaaa
    17281 taaacagttt tgtacgttct gcactatgcg aggaaggatt tttactaaaa aaaagccgct
    17341 ggggtttaaa acacccccag cggctcgttt tttacactat tgtctcagga attatctatc
    17401 gtccgtcgat tactcgatat ccctttcaat caacaatgaa tcatcccctt ccggagcaga
    17461 cattgggctg tattgccacg gattatggta gctctccatc gcctgataga tcacctgggc
    17521 cagctcatta ttactggacg ggtcatagca cagcaaatat tcggtatcag gtagcggcgg
    17581 taagccatct actccactca gcacacgcag gtccgggctc atcatctcaa ccggccttgc
    17641 cgtcacgcca agaccggctt tcactgccgc acgaacggcc ggaagcgtcg aggcgacata
    17701 agccagtcgc catggaatat ctactttatt aagcgtcgcc agcaccatat cgcgaaacgg
    17761 acttggatca tccagcaata caagagggat cggctcgcct ttttgcaata cgtattccgc
    17821 tgcgcagtac cagtgtgttg gcgaggtacg caggttcaat gccttaaacg ctgaaggacg
    17881 atgggtggtt accatcaaat ccacttcctg ggattccagc atttctgcca tataggcatt
    17941 acgtttaacg cggacatcca gcgctaattt cggataaacc gaactcacgc gatttaacag
    18001 gaaaggtaag atcgtatcgg cagattcatc tgaagcaccg atagttaaca cgccctgaag
    18061 attactgaac attaatgatg agcaggcctc atcattaaaa cgcaggattt tcctggcgta
    18121 accaagaagt tgaatgccat gttcagttaa cagtttgttg cgaccgtgac gagcgaacag
    18181 ttctttccca acgagttgtt ccagacgctg catttgctga cttacggcgg actgagtacg
    18241 acacacagcg gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa caaatgttct
    18301 cagcagatcg aggtcgaggt taattatcgg acgatttgca cttatcatat attatcactt
    18361 actggcggct catactgagc tggttaatgc tgtgcacaca caaacaagca attccatttg
    18421 taatgtgcct ccctggcagt ttcatccgga acccggacga aagtaaaaat gcatatgagt
    18481 tgcactaaaa aagcgactca cattgttccg ttataatgcc tgaagtagat cacagaatat
    18541 atcttcaggg atcgcatatc tattaagtta ctcactcttt tctatttatg acatgcgcgt
    18601 gtttgtataa atgtaaatgt gagtccttgt tccactctcg tgcagcatcg ctggtcatac
    18661 gcgaacacgt acaaacagca atggtgaagc ggcatcaaga gcagggatcc gtcatttatg
    18721 cgagcatttt accccaaaaa ccttttattt ataagggtca ttgcgaatta tctgatgcaa
    18781 agttatgtta tgttaggcaa agtaatcttc tattattaat aagcacatca aaaccttttt
    18841 gaatattaaa taataattaa ttagcatcat cctcattcat taattccgct taacaatagt
    18901 ttcacacctt acccccattg cgacacctcg gaaatcatca cgctgttaaa ctctccgtaa
    18961 tgcggaaata tcatcatcgc gtttcattag tgaattctta aatgaggcat tttcaaacaa
    19021 ttatcttaca gataaaaaaa ccagacttgc aattaagaat tagaacaatt cactatataa
    19081 cattgcatgt aaagcatata agcctcatta tttgtcatta ttaagttatt aacagcacaa
    19141 tcgagccttc ccctctggca aaatcttatt ctgcagacct tcaaaacacc gtcctggggg
    19201 agtacattgt tctaagctga cttccacggc agggagtggc gataacagca aaaaaggtca
    19261 agattcatgt cccccattga aaaatccagc aaattagaga atgtctgtta tgacatccgt
    19321 ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag gtaacaaggt actgaaactg
    19381 aacatcggca acccagcccc attcggtttt gacgcgccag atgaaatcct cgttgacgtg
    19441 atacgcaacc tgcctaccgc tcaagggtat tgcgattcca aagggcttta ctccgcgcgt
    19501 aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg atgttaccgt ggaagatatt
    19561 tacatcggca atggtgtatc ggagcttatc gttcaggcaa tgcaggcatt gctgaacagc
    19621 ggggacgaaa tgttggttcc tgcaccagat tacccactct ggaccgcggc ggtttcgctt
    19681 tccagcggta aagcggtgca ttatctttgc gatgaatcct ctgactggtt cccggacctc
    19741 gatgatattc gcgctaaaat tacgcctcgt acgcgtggga ttgttattat caacccaaat
    19801 aacccaaccg gcgcggtata ttccaaagag cttttaatgg agattgtgga gattgcacgt
    19861 cagcataatc tcattatctt cgccgatgaa atttatgaca aaattctcta cgacgatgct
    19921 gagcatcact caattgcgcc gctggcacct gacctgctga ccattacctt taacggactg
    19981 tcgaaaacgt accgcgttgc aggcttccgt caggggtgga tggtgttgaa cgggccgaaa
    20041 aaacacgcca aaggctacat cgaaggtctg gaaatgctgg cttcaatgcg cctgtgtgct
    20101 aacgttcctg cgcaacacgc cattcagacc gcgctgggtg gttatcagag catcagtgaa
    20161 tttattaccc ctggcggtcg tctttatgag cagcgtaacc gcgcgtggga actgatcaac
    20221 gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc tgtatatgtt cccgaaaatc
    20281 gacgccaaac gctttaacat tcatgacgat cagaaaatgg tgctggattt cctgttgcag
    20341 gaaaaagttc tgttggtgca agggacggca ttcaactggc cgtggccgga tcacttccgc
    20401 attgtcacgc taccgcgtgt cgatgatatc gagctgtctt tgagcaagtt cgcgcgtttc
    20461 ctttctggtt atcatcagct gtaatcttaa tttcactgcc ggagattgca tccggcagtg
    20521 ttatcccgcc acaatgacct gatgatgtca tcatacgtaa ggtcactatg aaacagagcc
    20581 atttttttgc ccatctctcc cgcctgaaac tcattaaccg ctggccgcta atgcgcaacg
    20641 tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc gatggtcgcc catgcgctgg
    20701 cagctatcaa aaatcggaaa tttggcggta atgtcaacgc cgaacgtatc gctttactgg
    20761 cgatgtacca cgatgcctca gaagtgctca ccggcgatct ccctactccg gtgaaatact
    20821 tcaatgcgca aatcgctcag gagtacaagg ctattgaaaa aatcgctcag cagaaactgg
    20881 tcgatatggt tccggaagaa ctgcgggata tctttgcgcc gttaattgac gagcatgcat
    20941 atagcgatga agaaaaatcg ctggtgaaac aggcagatgc actgtgtgca tatctgaaat
    21001 gtctggaaga actcgcggcc ggaaataatg aattcttgct ggcaaaaacg cgactggaag
    21061 cgacgcttga agcgcgtcgc agccaggaga tggactactt catggaagta tttgttccca
    21121 gcttccatct ttcgctcgat gagattagcc aggattcacc gctgtaagca gccggagtct
    21181 gcgtcgcatc agtcaataag cgccggatgc gacatcaggc tcttgtcaaa acggaaacag
    21241 catcgggatc atcaccacac aaaccgccat cacgataatg gtgaacggta cccccaactt
    21301 cacaaagtca ctgaagctgt aatttcccgg acctaaaacc agggtgttaa caggtgaaga
    21361 caccggtgtc ataaaggcgg cggatgctgc catcgccacg accatcgcaa atggataagg
    21421 cgacactccc atcgttttgg cagcagccag cgcaatcgga gccatcaaca ccgccgtcgc
    21481 ggtattagag ataaacagcc caataaccgc cgacaagaca aacaaacacc ccagcatcat
    21541 atgtggccca taaccgccgc caatatccat cagccctttc accgccagcg cgacacctcc
    21601 cgttttctgt aatgccacag caaacggcat catcccaacg atcaaaataa tgctcggcca
    21661 gtgaatggat ttataggcgc tttcagcatc tatacagcgg aatttcccca tcagcaggca
    21721 ggcgatgata gcggcaacag gattaggaat ttcatttgtc agcattaacg ccaccattag
    21781 caccagacag aaaatggcat ggggtgcctg gctgtgcgcg ggcgatgctt cactcacctc
    21841 ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg gccagcatac cgatcagttt
    21901 ccagttaccc acaaccagga tgatatcgcc cagcagcaga ggctcatccg ccagcgaacc
    21961 ttccagcgcc acgccattgc gcttgagccc caccacattc agtccgtagc gggtacgaaa
    22021 accaatttcg cgcaccgatt taccaatcag ttctgactca ggaattaatg aaatctctgc
    22081 catgcccaca tcaagggcct ggtcagaaaa atactcgccg cgcagtacca tcggctccag
    22141 caattgctca ctacaaaatt gccggagatc gacatcagcc gcagacatat caataagcaa
    22201 aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc acgataacgc gacgaaaacg
    22261 ccgccagcgt tcaacaccga tgacgttagc gccataacgc tcacgtaatt tgagatcatc
    22321 cagccgttga cctatcattg gcgatccggg gcgaatagcc agacgtcgcg cacgcccggt
    22381 cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc gtccagcctt cgcgctgcgg
    22441 ggtctgggta tcccctttca gcatgaaacg catcactaac atatacaaaa tacccagcac
    22501 cagcacgacc aggccaatag gtgttacgct aaagaaactg aagccgtgat agccttcacg
    22561 cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc accagcgtca tcatgccgct
    22621 aatcagcccg gcaaaactta acggcatcat cagacgtgac ggcgaagttt gcatacgcat
    22681 ggcaacgctt aacaccacgg gaataaagat agcgacaacg ctggttgaac tcataaacgc
    22741 gccaagcccc gcgacggtca gcatcaacaa aaccaacatt ttgatttcac tgttgcccgc
    22801 cactttgacc agccatgttc ccattacggt ggcaacaccg gtacggacca aaccatcgcc
    22861 aataataaac aaggcggcaa tcaggacaac gttaggatca gaaaagccgg aaaatacttc
    22921 ggggaccgtc agcgttccgc ttaatgcaaa cgcgacaata acaaacaaag cgaccgcatc
    22981 catacgcact ctgcccgtcg caaacaagac gatggcaacg gccagtaatg aaagaaccca
    23041 aatcaattca ccgttcacaa cttatccttg ttaattgagg gggatgactt gattctgcca
    23101 taaaaaagcc ccgacgagac ggggctaaat catgatcagg tgtttcactg aataataaca
    23161 tcgccatttg gctgtttggt cacagtaatt tgctccagac tgtggaggac caaatcgacc
    23221 tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa tgacatggca acccgccgcc
    23281 aggccagaaa gcacgccagc gggagcatct tccaccacca cacactcctg cggcgcaagc
    23341 cccagcagct gcgcacctaa cagatacgca tcaggttctg gttttccgcg cttcactcgc
    23401 tcagcggtta caaacacctc tggtgcggga agccccgcta ttttatggcg cgctcgcgct
    23461 accggcatgg agccagaagt cacaatggcc cacggaatac ctgctttatt caaatgattg
    23521 agtaaggcga ttgccccagg aagcgcggta ataccttcgg tttccgtggc ctcgatttgc
    23581 tccagacgcg taaactcggc ggcaatatca gcctcggatt tgcccgccat aaaatggcgc
    23641 aaagaggtga tcgcctgttt accgtgaatg aaagccagca cctcttccgg cgctaaccca
    23701 tgacgtctgg cccagttgct ccacgcccgt tctaccgcag gcagggaatc caccagcgtt
    23761 ccatcaagat caaacagaaa acctttgcac cgcacgcggg cctcctcagg cgttgataat
    23821 ttgattaatt tcgttggcgc tcaaatggta ctgacgcggg caggcatgcc acacattaag
    23881 catgcgctga tatttttccc acattggcgt ctgggcgtta aaaccatgag tcccagcgtc
    23941 aaaatgggta tagcgccctt ccacattaac cataaagcgg acataaccga ggtaacgtgc
    24001 ttcagtggcg gcgtcaaagc cgaggaaggt gacacgacgt tcatcgatgg attgctggtc
    24061 ctgcaaatta gaccaggaaa catacaacgc atgatacata tccataatgt cgatgatagt
    24121 gcggcaggtt tcttctttca gctcgccaaa ctcgcgatcc aattcacgca tctgtaatcc
    24181 gtaaccacgc tcaataattg tttgcaggcg acggtaacgt tcagcatttg ccggatcgag
    24241 catagtcatc atcttgtact gattagacaa aataagacgt tgcgcgttgg tcatttccat
    24301 tgttgactcc tgtatcactc tactacggtg aaaaaaaaga aggctgagta tgccttcttt
    24361 tatatgcgta atcaggggtc aattacaaat catcaaggaa agttttatcc agttgtttga
    24421 aggcgcgctt aagcgtgtca gctaatgcct ggtaatcagg cttgccttca acgggtgcca
    24481 acacctgtcc agactcctgc aatttaccgc gaacttcata aaaccagtta aggattgcag
    24541 ggggtaatgg cgttacagaa cgcttgccca gccaccacaa tccctgcatg ggtaaactta
    24601 aggcgaacag tgcagtggca actgccggcc caagcagacc gcccagggca atctgccagc
    24661 agagagtaaa tacggcgatc ggcggcataa aacggatcgc ataacgcgtc atcttgataa
    24721 cgcgattttc gacaaagacc ggagcaaggc gtttttccag tggccacgtc tttgagtaat
    24781 gctgtccgcg gcgaaacaag ctaaaaaaat taacagaacg attatccggc gttgacatgc
    24841 ttcacctcaa cttcacatat aaagattcaa aaatttgtgc aaattcacaa ctcagcggga
    24901 caacgttcaa aacattttgt cttccatacc cactatcagg tatcctttag cagcctgaag
    24961 gcctaagtag tacatattca ttgagtcgtc aaattcatat acattatgcc attggctgaa
    25021 aattacgcaa aatggcatag actcaagata tttcttccat catgcaaaaa aaatttgcag
    25081 tgcatgatgt taatcataaa tgtcggtgtc atcatgcgct acgctctatg gctccctgac
    25141 gtttttttag ccacgtatca attataggta cttccatgtc gagtaagtta gtactggttc
    25201 tgaactgcgg tagttcttca ctgaaatttg ccatcatcga tgcagtaaat ggtgaagagt
    25261 acctttctgg tttagccgaa tgtttccacc tgcccgaagc acgtatcaaa tggaaaatgg
    25321 acggcaataa acaggaagcg gctttaggtg caggcgccgc tcacagcgaa gcgctcaact
    25381 ttatcgttaa tactattctg gcacaaaaac cagaactgtc tgcgcagctg actgctatcg
    25441 gtcaccgtat cgtacacggc ggcgaaaagt ataccagctc cgtagtgatc gatgagtctg
    25501 ttattcaggg tatcaaagat gcagcttctt ttgcaccgct gcacaacccg gctcacctga
    25561 tcggtatcga agaagctctg aaatctttcc cacagctgaa agacaaaaac gttgctgtat
    25621 ttgacaccgc gttccaccag actatgccgg aagagtctta cctctacgcc ctgccgtaca
    25681 acctgtacaa agagcacggc atccgtcgtt acggcgcgca cggcaccagc cacttctatg
    25741 taacccagga agcggcaaaa atgctgaaca aaccggtaga agaactgaac atcatcacct
    25801 gccacctggg caacggtggt tccgtttctg ctatccgcaa cggtaaatgc gttgacacct
    25861 ctatgggtct gaccccgctg gaaggtctgg tcatgggtac ccgttctggt gatatcgatc
    25921 cggcgatcat cttccacctg cacgacaccc tgggcatgag cgttgacgca atcaacaaac
    25981 tgctgaccaa agagtctggc ctgctgggtc tgaccgaagt gaccagcgac tgccgctatg
    26041 ttgaagacaa ctacgcgacg aaagaagacg cgaagcgcgc aatggacgtt tactgccacc
    26101 gcctggcgaa atacatcggt gcctacactg cgctgatgga tggtcgtctg gacgctgttg
    26161 cattcactgg tggtatcggt gaaaatgccg cgatggttcg tgaactgtct ctgggcaaac
    26221 tgggcgtgct gggctttgaa gttgatcatg aacgcaacct ggctgcacgt ttcggcaaat
    26281 ctggtttcat caacaaagaa ggtacccgtc ctgcggtggt tatcccaacc aacgaagaac
    26341 tggttatcgc gcaagacgcg agccgcctga ctgcctgatt tcacaccgcc agctcagctg
    26401 gcggtgctgt tttgtaaccc gccaaatcgg cggtaacgaa agaggataaa ccgtgtcccg
    26461 tattattatg ctgatcccta ccggaaccag cgtcggtctg accagcgtca gccttggcgt
    26521 gatccgtgca atggaacgca aaggcgttcg tctgagcgtt ttcaaaccta tcgctcagcc
    26581 gcgtaccggt ggcgatgcgc ccgatcagac tacgactatc gtgcgtgcga actcttccac
    26641 cacgacggcc gctgaaccgc tgaaaatgag ctacgttgaa ggtctgcttt ccagcaatca
    26701 gaaagatgtg ctgatggaag agatcgtcgc aaactaccac gctaacacca aagacgctga
    26761 agtcgttctg gttgaaggtc tggtcccgac acgtaagcac cagtttgccc agtctctgaa
    26821 ctacgaaatc gctaaaacgc tgaatgcgga aatcgtcttc gttatgtctc agggcactga
    26881 caccccggaa cagctgaaag agcgtatcga actgacccgc aacagcttcg gtggtgccaa
    26941 aaacaccaac atcaccggcg ttatcgttaa caaactgaac gcaccggttg atgaacaggg
    27001 tcgtactcgc ccggatctgt ccgagatttt cgacgactct tccaaagcta aagtaaacaa
    27061 tgttgatccg gcgaagctgc aagaatctag cccgctgccg gttctcggcg ctgtgccgtg
    27121 gagctttgac ctgatcgcga ctcgtgcgat cgatatggct cgccacctga atgcgaccat
    27181 catcaacgaa ggcgacatca atactcgccg cgttaaatcc gtcactttct gcgcacgcag
    27241 cattccgcac atgctggagc acttccgtgc cggttctctg ctggtgactt ccgcagaccg
    27301 tcctgacgtg ctggtggccg cttgcctggc agccatgaac ggcgtagaaa tcggtgccct
    27361 gctgctgact ggcggctacg aaatggacgc gcgcatttct aaactgtgcg aacgtgcttt
    27421 cgctaccggc ctgccggtat ttatggtgaa caccaacacc tggcagacct ctctgagcct
    27481 gcagagcttc aacctggaag ttccggttga cgatcacgaa cgtatcgaga aagttcagga
    27541 atacgttgct aactacatca acgctgactg gatcgaatct ctgactgcca cttctgagcg
    27601 cagccgtcgt ctgtctccgc cagcgttccg ttatcagctg actgaactgg cgcgcaaagc
    27661 gggcaaacgt atcgttctgc cggaaggtga cgaaccgcgt accgttaaag cagccgctat
    27721 ctgtgctgaa cgtggtatcg caacttgcgt actgctgggt aatccggcag agatcaaccg
    27781 tgttgcagcg tctcagggtg tagaactagg tgcaggcatt gaaatcgttg atccagaagt
    27841 ggttcgcgaa agctatgttg gtcgtctggt cgagctgcgt aagaacaaag gcatgaccga
    27901 aaccgttgcc cgcgaacagc tggaagacaa cgtggtgctc ggtacgctga tgctggaaca
    27961 ggatgaagtt gatggtctgg tttccggtgc tgttcacact accgcaaaca ccatccgtcc
    28021 gccgctgcag ctgatcaaaa ctgcaccggg cagctccctg gtatcttccg tgttcttcat
    28081 gctgctgccg gaacaggttt acgtttacgg tgactgtgcg atcaacccgg atccgaccgc
    28141 tgaacagctg gcagaaatcg cgattcagtc cgctgattcc gctgcggcct tcggtatcga
    28201 accgcgcgtt gctatgctct cctactccac cggtacttct ggtgcaggta gcgacgtaga
    28261 aaaagttcgc gaagcaactc gtctggcgca ggaaaaacgt cctgacctga tgatcgacgg
    28321 tccgctgcag tacgacgctg cggtaatggc tgacgttgcg aaatccaaag cgccgaactc
    28381 tccggttgca ggtcgcgcta ccgtgttcat cttcccggat ctgaacaccg gtaacaccac
    28441 ctacaaagcg gtacagcgtt ctgccgacct gatctccatc gggccgatgc tgcagggtat
    28501 gcgcaaaccg gttaacgacc tgtcccgtgg cgcactggtt gacgatatcg tctacaccat
    28561 cgcgctgact gcgattcagt ctgcacagca gcagtaatct cgtcatcatc cacagctttg
    28621 cgctgcggat atctgaaccg gaaataatca ctatttccgg ttttttattc tcttaatttg
    28681 cattaatcct ttctgattat cttgcttaac tgcgctgcat caatgaattg cgccatctca
    28741 ctttgcatac ttaccacttt gttttgtgca agggaatatt tgcgctatgt ccgcaatcac
    28801 tgaatccaaa ccaacaagaa gatgggcaat gcccgatacg ttggtgatta tcttttttgt
    28861 tgctatttta accagtcttg ccacctgggt agttccggtg gggatgtttg acagtcagga
    28921 agtgcagtat caggttgatg gtcaaacaaa aacacgcaaa gtcgtagatc cacactcatt
    28981 tcgcattctg actaacgaag caggcgaacc tgagtatcac cgcgtacagc tgttcacgac
    29041 gggcgatgaa cgcccgggcc tgatgaactt cccgtttgaa gggttaacct caggatcgaa
    29101 atacgggaca gccgttggca tcatcatgtt tatgctggtg attggcggcg cgtttggcat
    29161 tgtgatgcgt acaggaacca ttgataacgg tatcctggcg cttattcgcc atacccgcgg
    29221 gaatgaaatt ctctttattc ctgcgctgtt tattctgttt tcacttggcg gcgcggtatt
    29281 tggtatggga gaagaggccg tcgcctttgc cattatcatc gcaccgctaa tggtccggct
    29341 gggctatgac agtattacca ccgtcctggt gacttatatt gccacgcaaa tcggttttgc
    29401 cagttcgtgg atgaacccgt tttgtgtggt tgttgctcag gggattgccg gcgttccggt
    29461 gctttctggc tccgggttgc gcatcgtggt gtgggttatc gccactctga ttggcctgat
    29521 ctttaccatg gtgtacgcct cacgagtgaa aaagaatcct cttctgtcac gcgtgcatga
    29581 gtccgaccgc ttctttcgtg aaaagcaggc ggatgttgaa caacgtccgt ttacctttgg
    29641 tgactggctg gtattgattg tcctgacagc cgtaatggtc tgggtgattt ggggcgtgat
    29701 cgttaatgcc tggtttattc cagaaattgc cagccagttc ttcaccatgg gtctggtgat
    29761 tggcatcatc ggcgttgttt tccgccttaa cggcatgacg gttaatacca tggcttcatc
    29821 ctttaccgaa ggggcgcgaa tgatgatcgc ccctgccctg ctggtgggtt tcgccaaagg
    29881 gattttgctg ctggttggta atggtgaagc aggtaatgcc agcgtgttaa atactatcct
    29941 caacagcatt gccaatgcca ttagcggtct ggacaacgcg gtcgcggcct ggtttatgtt
    30001 gctcttccag gctgtattta atttcttcgt gacgtccggt tctggtcagg cggcattaac
    30061 tatgccgtta ctggcaccgc ttggcgatct ggtcggtgtt aaccgtcagg ttaccgtgct
    30121 ggcgttccag tttggtgatg gcttcagcca catcatttac ccaacctcag cttcgttaat
    30181 ggcaacgctc ggtgtgtgcc gggtggactt ccgtaactgg ctgaaggtgg gcgcgaccct
    30241 gcttggactg ctgtttatta tgtccagcgt cgtagtgatc ggcgctcagt tgatgggcta
    30301 ccactaaaaa tgttaagagc cgcaattgcg gctctttttt cattctgccg tttcagtctc
    30361 tacggcttca tttttggcat tgcgcttcat ccacaacgcc agcgctttca gcgagtctgg
    30421 agtgaactca tcgcagcgtg cggtgatttc ttccggcgtc agccagcaaa ctccactgac
    30481 ttcatcttcc tgtagggcga agggaccgtg agagacgcaa ctgaacaacg cgccccagac
    30541 acggcaattt ttatcttcga aatagaactg cccgtgctcg gcaaagggga caccggcaat
    30601 gcccaactct tcttccgctt cgcgacgcgc ggattccagc agttgctcat cggcctgaac
    30661 tacaccgcct gcggtcgcat ctaacatgcc gggtaaaaag tcttttgtct cggtacgacg
    30721 ctggaccaga attttgccca tgccatcatg cacgacgatg taagttgcac gatgacgcag
    30781 acactgtgcc cgcatttgtt cccggctggc ttgtgcaatg acttcgttct cttcattgac
    30841 aatatccacc cattcagtac ttgccaaacg acgctgttcc attatcagga aaccttcttt
    30901 ttctggcgct cttacggcgc attttgagtt gtgggtaaat tacggattaa tcgcgacctg
    30961 cgcaatgata ctttgatcat tgagtgcgat tacactaagt acgtcattat ccagcatgcc
    31021 ataacccgcc ggattaccgc ctttcggaat actcaccgag ccggggttga agtggaaaat
    31081 ctccccccgc tgttctgcca ctggcagatg ggtatgaccg tacaccagca catcgttctg
    31141 gtttaaagca ggtagatttt ccgggccaaa aagatgacca tgcgtcaaaa acagacgttg
    31201 tttttccagt aatacctgtt gccacggcgc ggttatcggg aaatgcagca tcatttgatc
    31261 cacttcgctg tcgcagttgc cacgcacagc gataacctta tgtgccactt cattaagccg
    31321 ttcagcgact ttggctggcg cgtaaccctc cggtaaagca ttacgcgggc cgtgattcaa
    31381 cacgtcgcca aggatcacca gccactgggc accgctttgg acaaacaact ccacaacacg
    31441 ttccgtcgcc ggtaacgacc catgaatgtc cgatgcaaac atcagtttca tcactcactc
    31501 ctcgtcgaaa aaacgtaagc tatgatactg gattctgtcg ttgctatcag ccagattgct
    31561 tcgccgaaag tgcaataaaa cgctggacag acgctcgctg ccactggaat gtcgcataat
    31621 ccaccaggcg ttctggcacc tcatcgccat gtagtaccag gcggttaatc attagcgcca
    31681 gatcagtatc agcaatgcac cattcaccaa ataaattcgg ctgacccagt gctaacaaat
    31741 gttctgccat cgcgaacagt ttctctgcac tggcttttcc ctcggccgtt agtggcgctt
    31801 ttttcgcccc cgcaaagaca acatccgtcg gacgctcttc gcggatgggc atcagatcgc
    31861 tgcgcagcca ggcctgaatc tgtcgtgcac gcgcacgatt ttctaaatca agcggataaa
    31921 tacgttccca ggtcggtggc gcaaatcgat cttccagata ctccgcaatg gcagaagatt
    31981 cactcaactc aaaatcatcg atttgtaata acggcacacg gcgtgtctga ccgtaacctt
    32041 gccacgtcgg ctgcaaatgt tcaccgctgt cgaggtcgat ggtcttgata tgaaacgaca
    32101 ggcctttttc ctgcaacgcc acccaggcgg ataacacata aggggagaaa aagtgggcat
    32161 ctgaccaaag cgtgatagcg ggtttactca ttatgtcctc ggcaataaag ggggctggct
    32221 atcaaactat agagcctttt tgtcactgtc acgtgacgaa tactcacgta agtcgccgcg
    32281 ctttctatac ttgtggtgtt cagatgatta aacaaccgga gctgcaatga tcgatctcta
    32341 tttcgccccg acacccaatg gtcacaaaat tacgctgttt ctcgaagaag cagagcttga
    32401 ttatcgcttg attaaggtag acttggggaa aggcggtcag tttcgtccgg aatttttgcg
    32461 catttcgccc aacaacaaaa ttccggcaat tgttgatcat tctcctgccg atggcggcga
    32521 accgttaagc ctctttgagt ctgctgccat tttgttgtat ctggctgaga aaacaggact
    32581 ctttttgagt catgaaacgc gtgagcgcgc cgccacatta cagtggttat tctggcaggt
    32641 aggcggactg gggccgatgc ttgggcaaaa tcatcatttt aatcacgcag ccccccaaac
    32701 cattccttac gctattgaac gttatcaggt tgaaactcag cgtctttacc atgtactaaa
    32761 caagcggctg gaaaactcgc cctggctggg aggcgagaac tacagcattg cggatattgc
    32821 ctgctggccg tgggttaatg cctggactcg cctgcgaatt gacctcgcaa tgtatccggc
    32881 agtcaagaac tggcatgagc ggatccgttc gcgccctgcc accgggcagg cactgctaaa
    32941 agcacaactc ggtgatgagc gttcggatag ttaacagaaa caggttctcg tgtattattt
    33001 catcctaagt aaaacaacgg agaacctgca atggcacaac ctgccgctat tattcgtata
    33061 aagaaccttc gtttgcgtac gtttatcgga attaaggaag aagaaattaa caaccgtcag
    33121 gatattgtta tcaatgtgac gatccactac cccgccgata aagcgcgcac tagcgaagat
    33181 atcaacgatg cgctgaatta tcgcaccgta acgaaaaaca ttattcagca tgtagagaat
    33241 aaccgtttct ctttgctgga aaaattaact caggatgtgc tcgatatcgc acgtgaacat
    33301 cactgggtga cgtatgctga agtggagatc gataaactgc acgcgctgcg ctacgccgat
    33361 tcggtatcca tgaccttaag ctggcagcgt taatcgccat atcgggaggc tgcatgaaca
    33421 tagtgatcac cggagggacg ggattaattg gtcgccattt gattccgcgt ttgctggagc
    33481 tgggccatca aattacggta gtgacgcgta acccgcagaa agccagttcc gttctcggcc
    33541 ctcgggtgac actatggcaa gggcttgccg atcaaagcaa cctcaacggc gttgatgcgg
    33601 taatcaacct ggccggagaa ccgattgctg ataaacgctg gactcacaag caaaaagagc
    33661 gtctctgcca aagccgctgg aatatcacgc aaaaactggt cgatttgatt aatgctagcg
    33721 acacgccgcc gtcggtactc atttccggct cggcaacggg ctattatggc gacttaggtg
    33781 aagtggtggt taccgaagag gaaccgccgc ataacgaatt tactcataaa ctctgcgccc
    33841 gctgggaaga gattgcctgc cgggcgcaaa gtgacaaaac gcgagtgtgc ctgctgcgaa
    33901 ccggcgtggt actggcaccg gacggcggta ttctcggtaa aatgctgcca ccgtttcgtc
    33961 ttggcctggg cgggccgatt ggttccggtc ggcagtatct ggcctggatt catattgatg
    34021 atatggtcaa cggcattctc tggctgctgg ataacgagct gcgcgggcca tttaatatgg
    34081 tttcgcccta cccggtacgc aatgaacaat ttgcccatgc gctcggtcat gcgctgcatc
    34141 gcccggctat tttgcgcgtc cccacaaccg ccattcggct gttaatgggc gaatcttcag
    34201 tactggtatt aggtggacaa cgcgcgctgc ctaaacggct ggaagaagcg ggttttgcgt
    34261 ttcgctggta cgatttagaa gaggcgctgg cggatgtcgt tcgctgatgt ggtttacagc
    34321 aaacatccgc cagttaactc cctgtgttac aggattagtg gctttgcgcg ataagatcgt
    34381 ctggtgaaag tcgggtcacc atcataacta actctctgtc taaacctcta tccagcatct
    34441 cctgagcaat acgcagggct tcttcgtgtt tgccctgcat tgcgccttct tcacgtaatc
    34501 tgtcagcaat ggtcatcagt ttctcctttt cttgtggtgc gcgttctgtt atctcaccaa
    34561 taaatgcacg gaaacgctgg gcatcccctg tttgtaatac gtaattaaac agggctttta
    34621 gctgtctgtc attagtgttc cctgtaacta gcagcgaaac aatttggtcg actaatccca
    34681 acagatcgcg ctgacgaata tgtttctgaa ttaactccaa cagcgccatt ttgcggtgtt
    34741 gcataatctc gtcatccggc accacggtaa tatccaccaa cggaaacgct gatgaatata
    34801 ttttgcgggc tatagcaggc tcggcaaatt catcaagcca gcagagtgaa taaggataag
    34861 gacttctgca accatgataa aacagcatcg ggatcaccaa tggaagctct ttatagcccg
    34921 catcaagatg gttttgcatt gccgcaatgg aataacgcat catgcgaaaa gccattaatt
    34981 cttccggctt actttggtgc tctatcacta cataaatata acccactccc tcctgcgttt
    35041 tcacagacca caagaggtcg gaataatatt gccgcaggtc ttcatcaata aaactgtttg
    35101 gttctagttt aagcgtcgtt aaatcacaca gtttgcgcag cggcgcggga agatgaatat
    35161 taataaaatt ccgcgcggtg tctggatggc gtaaaaaaga tttaaatacc gcatcatgcg
    35221 gcgtggaagt tgtcgatatt gtcatggcgt tccgtcaccc ctcaaattta agatgacgcg
    35281 acaataacca cgattaacac ccaatgcagc aggcagtttt tatctttgga cagcgaattc
    35341 agaagtattt ttagcggact gcaactttta catttcagcg gaagctgact cgcaaattcc
    35401 ccttgaaaat actccggcag cgttagccac cggagaggaa attatttcag cgatccctta
    35461 aggaactgtt gcagacgagg gctttgcggg ttgccaaata actgctccgg cgcgccctct
    35521 tcttctattt tcccctgatg gaggaaaatg acgtgagtag aaacatggcg agcaaagccc
    35581 atttcgtgag tcactaccac catggttttc ccctcttctg ccagttgctg cataatacgt
    35641 aacacttcgc ctaccagttc aggatcgagc gccgaggtag gttcatcaaa cagtaaaact
    35701 tctggttcca tcgccagcgc ccgcgcgata gaaacacgct gttgctgacc gccggaaaga
    35761 tgcaccggat atttcccctg cgcacgttcg tctatcccga ctttcgccag atacttcacc
    35821 gcccgctcgc gcgcttcctg cttgctcagg cccaacacct gaatcggcgc ttccatgacg
    35881 ttttccaaca ccgtcatatg gctccagaga ttgaagtgct ggaataccat cgtcaggcgt
    35941 gtgcgcagta agcgcagttg atttttatcg gcgactttga gttgaccgtc tttgtcgcgc
    36001 accagattga tcgtctggcc attgaccacg atcgatcctt cactcggttt ttcgaggaag
    36061 ttaatgcagc gcagaaaggt acttttcccc gatcccgacg atccgatgat gcttattaca
    36121 tctccggcat tcgcttgcag tgagacccct ttcagcactt cgtgttcgcc atagcgtttg
    36181 tgcaaatcga taacgtttaa tttattctcg gacatcgtat tctcagtgcg ttgaagaagg
    36241 tttcacatgc tgcaaccagc gtttttccgc tctgcgaaag aggctgatca ggacataaga
    36301 gatgattaaa tagagcaccg cggcaatgcc gaaggcggta aaaggttgat acgtggcggc
    36361 gttaatatcg cgggctattt tcagcagatc cggcacagtg gccgtaaacg ccagcgcagt
    36421 agagtgcagc atcaggatca cttcgttgct gtatgccggt aacgcaatac gcagcgcaga
    36481 aggcaaaata atgcagcgat acattttaaa ggtcgagaag ccataggctc tggcggcttc
    36541 aatttcccca tgcggaaccg aacggattgc cccagcaaaa atctcggtag tgtaagcgca
    36601 ggtgttaagc gtcagcgcca gcacggtaca gttcaggcca ctgcggaaga aagcgttaag
    36661 gaattcggtt cccttaacaa tctcaagcgt gtacatgccg gaatagaaca ccagcaactg
    36721 aacatacagc ggcgtaccgc gaaaaatata ggtaaataac cagattggaa actggatgta
    36781 tttattactg gagacacgac caatcgccag aaacagcgcc aggactccgc ctatcactac
    36841 cgacaaaata agcagccaca gagtgatcgc cacaccagta aagcgataac cgtcggtcca
    36901 cagcagcggt ttccagtatt catgtaagat ttcgatcaca ggtcagccct cttcacaccc
    36961 acggagtagc ggcgctcaag gaacagcagc acaccattgg aaacggtggt gaaaaccagg
    37021 taaatcacgc cacagacgat ggcgaaatag aacggttccc aggtactttt gcctgccagc
    37081 tgcgtggctt tgaccacatc ttccaggccg agtaacgaaa ccagtgcggt agatttgagg
    37141 atcacctgcc agttgttgcc aatgcctggc agggcgtaac gcatcatcgc cggaaacatg
    37201 atccgccgaa acacttgccc gcgagtaaaa ccgaacgccg tcgccgcctc tatatgccct
    37261 ttcggcactg ccataaacgc gccacggaac gtttcggtaa aataagcacc gtagataaaa
    37321 ccgagagtga taataccagc gaccatcgga tcgatatcaa tctgcccgac gcccatcgcc
    37381 tccgtcacca tgtttagcgc aatctgcaaa ccatagaaaa tcagcagcat caacaccaag
    37441 tccggcacgc ctcgaatcag cgtggtgtac ccttcgaaaa tcagccccga aagccgattt
    37501 tgcgagagct taccgccagc gccaattaaa ccgatgatta cagcgagcac tacagagctg
    37561 atagccagct ccagcgtgac gagcgcaccc tgtaaaataa cacctgaaaa cccatacaac
    37621 atgctgcctg tcctgtcgtg tgtggtgaat taccgcctct tgtctccctc cggttacccg
    37681 aagggagcgg gctttctttc accgatggcg attaaccacc ataaacatca aaatcgaagt
    37741 actttttcgc taatttctcg taagtaccgt cagcgcgcat ttcggcaaag gctttgttca
    37801 gtgcctcgcg cagttcgtta tcttctttac gcaggcccat gccggtccct acgccaaaca
    37861 gtttttcatc tttaacagac ggaccaccga atttgtaatc tttaccgacg ggttgtttga
    37921 ggaaaccttc gctggcagcg acctcatcct ggaacgcggc atcaatacgt ccggcagtca
    37981 ggtcagaata aatgttgtcc tgcccctgat acgagacgat ttcaatgcct tttggtgccc
    38041 agtgttcatt accgaacgtc tcctgggtgg tgccctgcaa tacgcctacc cgtttgcctt
    38101 tcagcgactc gactgtcggc tgaatgtcag aatttttcgc caccaccaga cgagaatcgg
    38161 cagcgtacag tttgtcggtg aaggcaattt cttgctgacg tttttccgta atggaaagcg
    38221 atgacatgat ggcgtcaatc ttcttcgctt ttaaggacgg gattaacgca tccagcgggt
    38281 tttcgacaaa cgtacattgc gtattgatgc gcttgcacaa ctccttcgcc agctcgatat
    38341 cgaaaccaac cagttcgcct tgtgaatttt ttgattcaaa tggcgcatag gtcgggtcgg
    38401 taccgatgcg gatgttttgc ggaattgcag caagtgccgc agttgcgctg gagaaggcca
    38461 gaaccagaga gagcgatagc accagttttt tcataatgtc ctcaactgac agtcttttta
    38521 tagggaatat ttacaggttt gttacactta tcgtgccata aaattgacca tcaaggcaaa
    38581 atatcccgcc ccgttagacg tattattttt cattaaaaat gcttaagtat gcatttcaat
    38641 gcaccacgat agtgcaacaa acctggcgtg caccatgatg atcattccat caggtacagc
    38701 ttcccagcga cgtatcagtc accgtagaca ttaaagtcga aatacttttt cgccatcttg
    38761 tcgtaggtgc cgtcctgacg cagctcgcca agcgccttat tgaaggcagc cgtcagttca
    38821 gcataatctt tacgtagccc tacaccggtg ccatcaccga agtatttttt gtcttttact
    38881 gatgagccag caaaagcgaa atctttacca gcaggttgct tgaggaatcc ttcgctggca
    38941 gcaacttcat cttgtaacgc agcatccaga cgtcctgcag ccagatcgga atagaccaaa
    39001 tcctggttgg cataggccac cacatccacg cctttactac gccaggtctc gttagcgtaa
    39061 gcttcctggg ttgatccctg cagcacacca acatgtttac ctttcagtga atccagcgtt
    39121 ggctgaatcg gtgaaccttt ggcagcaatc aaacgagaat ctgcggcgta cagcttgtcg
    39181 gagaaggcaa tctcctgctg acgtttatcg gtaatggaaa gcgacgaaat aatagcgtcg
    39241 atttttttcg ctttcagtga ggggatcagc gcgtcaaagt cactggcaac ccaggtacat
    39301 ttcacctgca tccgtttgca catctcgtta ccgagatcga tatcaaagcc aacaaaatca
    39361 cctttagcat ctttcgacga gaacagcgca taggtggtat cggttccgat acgtaccgtc
    39421 tccggtagcg ccgcatagct ggaagccgct gcggagagac cgactaataa agacagagcg
    39481 agaatcgact tcttcataca taaccctcaa gtgaaaatgg cttttttatg ttgtgtattg
    39541 tgttgtgtgt ttgcaggctc tttcatgcag gtcttatgcc atcttgacga caacagtaac
    39601 attcaacgtt aaatatgtta ataagacatt gcattattgt cctgaagttg aagatagcag
    39661 gtatagcggt tgaatcgcag cgtttcgatt gaatggcaga aacaaaatgt cgaggatttg
    39721 atcgcggttg caaaattgcc ctgaaacagg gcaacagcgg agttatgcgc cctgccagcg
    39781 ggcaaagaga tcttcaggaa gggttattgc aaactggtca agaacacgat taaccgtctg
    39841 atttatcaca tcatcaaggg attgcgggcg atgataaaac gccggaacgg gaggcataat
    39901 caccgcaccg atttctgccg cctgagtcat taaacgcaga tggcctaagt gcaatggtgt
    39961 ttcacgcacg cagagcacca acgggcgacg ctctttcagc accacatctg ccgcacgggt
    40021 cagtaagcca tcagtatagc tatggacaat gccggacagg gttttgattg aacagggtaa
    40081 aatcaccatc cccagcgtct ggaaagaacc ggaagagatg ctggcggaaa tatcgcgcgc
    40141 atcgagcgtg acatcagcta atgcctgcac ttcgcgcagg gaaaaatccg tttcgaggga
    40201 taaggtctgg cgcgccgcct ggctcatcac cagatgcgtt tcgatatctg tgacatcgcg
    40261 cagaacctgt aataagcgca cgccataaat cgcgccgctg gcaccgctga tgcctacaat
    40321 gagtcgtttc ataaatagtt gccctgtcag acttgcaggc agactttgca gtatttcggc
    40381 gggagttgca agtcagggtg ccagaccggc accctcagcg aaggcatcat ccttcgttat
    40441 gcatttcgag attttccact tcgttctgac gttgcactgc tttggcgtca tcattacgta
    40501 acgtatcgag gaaatcgagg tagccctgat caacatcttt ggtgacgtag acgccgttga
    40561 acaccgagca ttcaaactgc tggatatccg gattttcagc gcgaacggcg tcgatcagat
    40621 cgttcagatc ctggaaaatc aacccgtcag caccgatgat ctggcgaatt tcatcaactt
    40681 cgcgaccgtg agcgatcagt tccgtggcgc tcggcatatc aataccataa acgttcggga
    40741 agcgaatttc cggtgccgca gaagcgaggt acactttctt cgctccggct tcgcgtgcca
    40801 tctcgataat ctgctcagaa gtggtgccac ggacgatgga gtcgtcgacc agcaggacgt
    40861 ttttatcgcg gaactcggcg cggttggcat tcagtttacg gcgcacggac ttacgacgca
    40921 gctgctggcc cggcatgata aaggtgcggc caacatagcg gtttttaacg aagccctggc
    40981 ggtacggttt gcccagaata cgagcaattt ccagcgcgat atcacacgag gtttctggga
    41041 tcgggatcac cacgtcgata tccagatctt cccattcgcg ggcaattttc tcgcccagtt
    41101 tcgtgcccat attcacacgc gcgctgtaaa cggaaatttt gtcgataaac gagtccgggc
    41161 gggcaaagta tacatactca aacaggcacg gattgctgac cggattgtca gcacattgac
    41221 gggtaaacaa ctgcccttct tcagtgatgt aaatcgcttc gcccggcgcg acgtcacgca
    41281 ggaaatcaaa gcccagcgta tcgagcgcta cgctttcgga agcgaccata tattctgtac
    41341 ggttctcgtc aatatcacgt tttcccagta ccagcggacg aatcccgttt ggatcgcgga
    41401 aagcaaccat accgtggccg ataatcatcg ccacacaggc atacgcgccg cggattaagc
    41461 ggtttgtggc agcaatggca gcgaaaatat tgtcggcttc cagcgggtag tggcggaagt
    41521 tgtccagctc gctggcgaag atattaagca gaatttccga gtcggaagtg gtgttgatgt
    41581 ggcggcgttt ttcttcaaac agttttttac gcaactcgtg agcgttggtc agattgccgt
    41641 tgtgggcaag cgtaatgcca tacggggagt taacgtaaaa cggctgcgct tcagaggcgc
    41701 tggagctgcc agccgtaggg taacgcacat gaccaatgcc catattgccc tgcaaacgct
    41761 gcatatggcg agcttcaaat acatcgctca ccagcccgtt cgctttacgc aaacggaagc
    41821 agttattggc atctatggtg atgatgccgg cggcatcctg accgcgatgc tgaagcaccg
    41881 ttaaggcatc ataaatcgac tggttaaccg gcataacacc ggcgataccg acaataccgc
    41941 acatacgtct ttttcctcgt taagccacat ctcagagcac ttacgctctg ggcaagaaac
    42001 ttgacgagct ttgcagataa tcaaaaaacc atctgatgat aaaactgaat tgcgggatca
    42061 gctgtgattt gctccagtct tcacttttcg acaccccggt aaaggagtcg agaaagaaga
    42121 gaatggcggc aacaatcaac acaccgcgca acgcaccgaa acagacgccc agcacccgat
    42181 cggtgcctga caaccccgtt ttctccacca actggcctat cacgaagttc acgatagcac
    42241 caacgatcag ggtagcgata aacagtaccg cgatggcaat cccatttcga accagttcgt
    42301 cttcaaagcc cgtaaaccag actgacaggt aagtgtagta atgactggca acaaagaaag
    42361 cacaacccca tgtcaccagc gataacgctt cacgaacaaa gccgtggatc aggctaacca
    42421 gagaggaaaa agcaatcacc gcgattatgg cgtaatcaat ccagaccata tgtgtcccac
    42481 gattttacgc cctgtcatcc tgttcggggc gcattctaac agaaaaagaa aacgtttgcg
    42541 tagggatttc cttcccgcgc atcaataaaa atggcgctga aaaaatattc aacgccatcg
    42601 actttttatg cctttgcggc atcgtgcaat gcgtgtcgga tgcggcgtaa acgccttatc
    42661 cgacctacgg ttctaacact gcccaagcct gataagacgc gccagcgtcg catcaggcaa
    42721 gactgtatta attcggcgta tagcccatta ccacgccact taagccagaa agttgcttca
    42781 actcacccag cgaacctttc agcttatctt tcgaggcatc cggcccaacc agaatacggg
    42841 taattttacc ctgcactggc gtggatggcg acgtataaac ccgataaccg gcaccgcgca
    42901 gcttaccgac aatctcattc actttatcgg catttttcag cgcacccagt tgcacaacat
    42961 aggctttacc cgttggcgct gctttttctt ccacgaccgg cttcggttct ggcttcggcg
    43021 caggtggtac ttcaaccttt ggtttaggcg gctccaccgg tttcggcttc ggtggggcga
    43081 ccggtgcagg ttccggttca aactcggtgt tattggctgc aatagtggcc ggatcgagcg
    43141 acggtgctgc ggcatcacct gcccgcacct cttccgctgc gccttccggc ggctgcgtcg
    43201 gcaacgcctg ggtggcggct ggcatcatat caggctcatc acgatcgccc gctttcggca
    43261 ccagcgggat agccgcgaac tcatcctgat aatgtttttt ctgcccgtcc agcagccctg
    43321 gaagtacaat cacccccagc gccaccagca cgatcgtgcc cactaaccga ttctgaaact
    43381 tacttgccac cgcttctcct cgcgtcaatc acttccatga catgtgcgac cgtgtggaaa
    43441 gaaccacaca ccagcacggt gtcttccgct ttagcgtccg ccattgcggc atcccatgcc
    43501 tgcgcaacgc tatcaaatga tttgccgtta cccaaatgct caagcagttg ttctgccgtg
    43561 gcaccgcgcg gcccttccag tggcgcacaa taccagtcat caaccacgct tttcaaccag
    43621 gccagagttc cggcaatatc tttatcatgt agcataccga taaccgccag cacgcgcccg
    43681 tttttcggta gcgctttcat acgcccggtg agatattccg ccgcatgtgg attatgcgcg
    43741 acatcaaaaa taacgcgtgg cgactcgctc acaatctgga aacgtcccgg caaaattgcg
    43801 ctggcaatcc cgtcgcgaat ggcattttca ctgacttcca gcccgctggc acgcagtgcc
    43861 gccagcgccg ttgcggcatt cggttgcggg acaagcggca acggcagatt ttccagcgtg
    43921 ccgtgagcat cgctaaacgc ccagtcatga tcggtgacgg aatagttcca ctcaacgccc
    43981 cgacgttgta acagtgcacc tttttcctgc gccacatcag caatggtaga aggcatttcc
    44041 ggctcaccga caattgccgg tttttcgctg cggaagatgc ctgctttctc gagaccaata
    44101 ctttcgcgat ctggacccag ccagtcggta tgatccagcg caatactggt tactaccgcg
    44161 acatcggcat cgacaatatt ggttgcgtcc agacgaccgc ccagccctac ttccagaatc
    44221 accacgtcaa gttgtgcctg cttgaacagc cacaacgccg acagcgtacc gtactcgaaa
    44281 taggtcaggg aaatatcacc gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc
    44341 gattccggca attcctggcc ctgcacacgt acgcgctcgg tataacgcac cagatgaggc
    44401 gaactgtaga cgcccacttt gtaccctgcc gccatcagaa tcgactccag cgtacggcag
    44461 gtggtgcctt tgccattcgt acccgcaacg gtaaacacaa atggcgctgg tttcaggacg
    44521 ccaagacgcg ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt tttactgtgc
    44581 aggttttcca gataagaaag ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg
    44641 ataatcatgg tatccgctga ttcgttacgg tgagaatagc aaaagggcag agccagtggc
    44701 cctgccctta tcagttatca ggcctcaggt tcctgatccg gtaccggggg taccactacg
    44761 ccttcacgcg gcgcttcagg attcggcgct ggcagattca tcaacttcgc cagaatgctc
    44821 gccagtttca ggcgcatttc cggacgacgg acgatcatgt cgatcgcgcc tttctcgatc
    44881 aggaattcac tgcgctggaa tctaggcggc agtttttcgc gaacggtctg ttcgataaca
    44941 cgcggaccgg caaagccgat taacgcttta ggttcagcga tgttgagatc gcccagcatg
    45001 gcgaaacttg cagaaacacc gcccatcgtc gggtcggtca gcacggagat gtacggcaag
    45061 ccgcgctcct gcatttttgc cagtgccgca gaggttttcg ccatctgcat cagcgacatc
    45121 agtgcttcct gcatacgtgc gccaccagag gcggagaagc agatcagcgg gcagttatct
    45181 tcctgcgcct gctcaacggc acgcacgaaa cgtgcaccca caacagaccc cattgaaccg
    45241 cccataaagg cgaactcgaa tgccgcagcg acaaccggca ttccatacag agtgcctttc
    45301 atcaccacca gcgcatcttt ttcgccggtt tctttctgcg cagatgccag acggtcttta
    45361 tacttcttgg agtcacgaaa cttcagctca tctttcggct caagctcgct acccagctcc
    45421 acaaggcttc cttcatctaa caggctatgc aggcgattac gcgctgtcat acgcatgtga
    45481 tggtcacact tcggacagac ctcaagatta cgttccagct cagcgcggta taaaacctga
    45541 ccgcagctat cacacttagt ccacacccct tcaggaatgc tcgccttgcg ggtgggagta
    45601 atgttgcttt taattcgttc aatccagctc attagggacc tttctgtctg aacctggttc
    45661 gatgccagtt ttatctttgg ggacgcataa tgccattttt gcccccaaca gaccatgaat
    45721 gttgcacatt aaaacataac agcccgaaac tttggataaa aaagtggtcg aaccgcggag
    45781 ttacttttta ttttgcggcg cgtgccgcag cgcgtttgtg acggattatt tcgatgacgc
    45841 ccggcaaaat agaaaccaca ataatcccga cgatcagcag cttaaggtta tcctgaacca
    45901 tcgggattgt accgaagaaa tagcctgcgt aggtaaaaag cagtacccac aacagtgcgc
    45961 cgatcacgtt ataagcagcg aaatgacggt acgacatgtg gcccattccc gcaacaaacg
    46021 gggcgaacgt tctgacgatc ggcacaaaac gggcgagaat aatcgttttg ccgccatgtt
    46081 tctcataaaa ctgatgggtt ttgtcgagat aactgcgacg gaaaattttc gaattgggat
    46141 tactgaataa cttttcaccg aacaaccgcc cgatagtgta attgaccgcg tcacccacaa
    46201 tcgcggcgat cagcatcagc actaccatca tatggacgtt gagatcgttg gtttccagtg
    46261 atgccaacgc tccggcaaca aacagcaatg aatcacccgg taaaaacggc attaccacca
    46321 gaccggtttc acagaacaaa attaagaaca aaatggcata aacccagacg ccgtactccg
    46381 cgactagctc tgccagatgc acgtcaatgt gcaggataaa atcgatgagg aaataaatca
    46441 ggtccatatt tgcctatgtc ttgtactcgt catctttcag gctgtaacta cgttggctac
    46501 agcctgaaat gttccgagca ttattctcgt tagtccgcca gaaatagcgg gcccattggc
    46561 ggttttggaa gatcataccg gtcagggtaa tccaccgcga ccagatacag cccttccgct
    46621 tttgccgttg ctgccgccag cgttctgtcc tttgccgcca gcaactctgc tatccagctc
    46681 tccggctggt tgtgggcacc gacttccatc aggctgccga caatattcct gaccatatga
    46741 tgtacaaagg cattcgcttt gatatcaacc accacataag gaccgtgacg cgtgacatta
    46801 atatgcataa cgttgcgcca cggggttcgg gactggcact gcaccgcacg gaacgaggtg
    46861 aaatcattct cgcccagcaa gcattgcgca gcccgatgca tccgttcagc gtccagcggt
    46921 tcgtaaaaat gggttacccc tttactcagt accgccgggc gcagccgatg attgtagatg
    46981 atgtagcgat aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc aggtacagct
    47041 ttaacccaac gcacggcgat gtcaccaggt aaattcgcat ttacgcccaa ggtccacgcc
    47101 gcgtctttgc gctgcgcggt tgtttcgaaa tgcaccactt gcccggtacc gtgtacccct
    47161 gcgtcagtac gcccggcgca gaagacggtg atgggttcgt tcgccacctg ggagagcgcc
    47221 ttttccagct tctcctgcac actgcggact tcattctgcc gttgccagcc gtaatactta
    47281 ctgccgtcgt actcaatgcc cagcgcaatt ttataaactg gcagttgttg ctggtcggac
    47341 attagtacag atactcctgc accagtttct cggcgatttt tactgccatc agcgcgccgc
    47401 caaagcgaac gttatcggcc accgaccaga actggacttg ctccggcata ccgtagtcat
    47461 tacgcacgca gccaacagaa agatgcggcg tacccgaagc atcccctacc tgagtcggga
    47521 attcgttctc ttcagagagc acaatatctt cgccctgagc aaacgcatca cgcgcttctt
    47581 ctgccgccag cggacgcagt gcttcaaagt tgaccatctg ggcatgaccg tagaataccg
    47641 gtgcctggac gacgcttgcc gaaatcatca gcccttcgtc ctgcaggatt ttgcgtactt
    47701 cgtcaacgat acgacgttct tcacgcacgc taccttcgct atccggcaac aacggcagca
    47761 tattgaacgc cagttgacga ccgaagaaat cttcttcgtc aatcggaatg ccgttgagca
    47821 atttcgcact ctgccccgct aacgcatcga ccgctttttt gccctgggcg gaggctgaaa
    47881 tcaggctggt aacactgata cgcgacaaac cgccctgatc gattaacggt ttcagtgccg
    47941 ccagcagctg gctggtcaga ctgtctggta cggcgatgac attccggttg cggtaatcgg
    48001 tcagtacaaa cgggtttact tccggcacca ccagcggtac gtcgggttcg agagcaaaca
    48061 gtccgctgct gtcgatcacc aggcaacctg agttggtcgc ttcttcaacc caggcagcgg
    48121 tagcttcttt gcctgcgaca aaaaatgcca gctgcgcctg cgtccagtcg aattcagcgg
    48181 catcctgcac ggtgattgtt ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac
    48241 gtgccagtgc ataaatttcc ccaaccggga actgacgttc agccagcgtt tcaagcaggg
    48301 cttcgcccat agcgccagtt gcgcccagga cggcaatgtt ccagccttca gacatggtgg
    48361 tttactccag aaatagcaaa gctccctgcc aatgttacag cagagagcat gaagaagaga
    48421 ttaacgtgcc ggatgatgaa cggcgttaaa acccagttta cacagcaatg atgccgcact
    48481 ggcgtcatca caaattacat acagagatga ccattcacgg cgctcaagat agtttttgcg
    48541 cagtttatcg aactcacccg gtatcccggc gactttacgc agcggtgcgt catcgcggcg
    48601 cacatcatac accaaatgca ccagcctttt cagcgtcggt tgatcgagcg gaccatgcag
    48661 cgtaatgcgg ccaaactcag gcgcgggcag caacgtatcc agcgcaacgt gttgttcatg
    48721 cccaataaac ttgctatagg cttcaaacac ttgcgtcgtg ccacgcgctt taccctccag
    48781 ggtatagcct gcgatatgcg gcgtgccgat atccactttt gtcagcaatt ccacattaag
    48841 ttccggctcg ccttcccaga catccagcac cacgcttaac ttctggcctt cattcaggca
    48901 ggtcagcagc gcggtattat cgacgactgc gccacggcag gcgttaatca gaatcgctcc
    48961 gggcttcagg ctacggatca gtttttcatc cgccagatgt agcgttttgt acggaccatc
    49021 tttaaagagt ggcgtatgga aagtcagaat atccgcgcgc tggactaact catccagcga
    49081 gcggaaatca ccctcatccc cacggtcggc gcgaggcgga tcgcaaagta aggttttaat
    49141 ccctaacgct tccagtcgcg cctgcaatcg acgtccaacg ttaccaacgc ccacgatccc
    49201 caccgtacgg tcgtgcagtg aaaatccatc gcgttcggca agcatcagca gggaggagaa
    49261 aacatattcc accaccgcaa tcgcattaca gccaggtgca gcggaaaaac caattcccgc
    49321 ctgcttcaac catgcttcat cgacatggtc ggtccccgca gtggcagttc caacaaattt
    49381 aatgggtttt cctgccagca aagattcatt cactttcgtg accgaacgca ccatcagcgc
    49441 atccgcgtct gccagttgag cgacggggat tggacgcccg ggaaccgcga tcacctcacc
    49501 caaacggcta aataagtcgc gggcataagg catattttca tcaacaagga ttttcacgtt
    49561 tgtgttacct gtatgagacg agagttaacc ggacaagtat gccataatct cgcggccagg
    49621 catacttgcg aagatttcag gtataaggat acgtaatgat acaacctatt tccggccctc
    49681 ctcctgggca gccaccaggt cagggagata acctgccgtc tggcgcgggc aatcagcctt
    49741 tatccagtca gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc
    49801 aacaacaaag agcagaactg tgggcgggta tcaggcacga tattggtctg tcgggagatt
    49861 caccgctgct ttcgcgtcac ttccctgccg ctgagcataa tctggcgcaa cgtctgctgg
    49921 ccgcgcaaaa aagccattct gcccgccagc ttttagcgca attaggggag tatttacgtc
    49981 tggggaatca tcgtcaggcg gtcacggatt atatccgtca taactttggt cagacgccgc
    50041 tgaatcagct ctcaccggag caattaaaaa ccattctcac cctgttgcag gaagggaaga
    50101 tggttattcc gcaaccacag cagcgcgagg cgaccgaccg tcctttatta ccggcggagc
    50161 acaatgcgct aaaacagctg gtgaccaaac ttgcggcggc aacgggggaa cccagcaaac
    50221 agatctggca atcgatgctg gaactttccg gggtgaaaga tggcgagtta attccagcga
    50281 aactgtttaa ccatctggtg acctggctac aggcgcgtca gacgctaagc cagcaaaata
    50341 cgccgacgct ggaatcacta cagatggcgc taaaacaacc tttagatgcc agtgaactgg
    50401 cggcgttatc ggcatatatc cagcaaaaat atggcctttc tgcgcaatca tcgctttctt
    50461 ctgcccaggc cgaggatatt cttaatcagc tttatcaacg gcgggttaaa gggattgatc
    50521 cgcgtgatat gcaaccgctg cttaatcctt ttccaccgat gatggacacg ttgcaaaata
    50581 tggcaacgcg tcccgcgctg tggatactgt tagtcgcgat tatcctgatg ctggtctggc
    50641 tggttcgtta accccgacga aatgacagta tcgtgacaat aatacccagc accgcagaga
    50701 tcgccccggc aagaaatacc gaagagtaac caaacgtggt cgccagcatt cccgccagcg
    50761 gcccggagac gccgagggcg atatcctgaa acgcggcgta accgcccagt gcggtgccgc
    50821 gaacttgtga ggggacgcgt ttaaccacct ccacgcccag cgcaggaaag ataagcgaac
    50881 atccggctcc ggttaacgcc gcgcccgcta atgcgaccca tgcacctggg gcttgccaga
    50941 gcagcaacaa gcccaccgtt tctacaagca gagagacaat cgccactttc acgccgccaa
    51001 aacggtccgg catccagcca aacatgacgc gcatcacgac aaatgcgccg ccaaacgcgg
    51061 taagagtaaa gcccgccatc gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc
    51121 cgataaccgc aaaaccaacg ccttgtagtg ccagccctaa ccctggtttc cagataagcc
    51181 cgacaacgct ccacagcgat ggacgttctc ccgccagggc cggtactttg cgcactgtgc
    51241 cgttacaggc ccacgccagt aagggtaata ccattgtggt gatcgccagt gcggcaaaac
    51301 cgtaatggct atgaatcaac aggccaagcg gcgcaccaac agcgagggca ccgtaaatcg
    51361 ccattccgtt ccatgacatc actttgccag agtgttttgg ccctacgatg cctaaccccc
    51421 aggtcagagc gcctgtcagt aactggcttt caccaaaccc aagaatcaaa cgcccgatga
    51481 ccaacagggc aaatttgaac ggtgcggaga caggcaaaat cgccgccagc agcaacgcgc
    51541 cgccagccag accacaagct aacatcccct gaagcgccga acgttttgcg ccatattgat
    51601 cggccagtcg cccggcgtaa ccacgcgtca gcaccgtagc cagaaactga atcccgacgg
    51661 caatgccgac catggtattg ccatagccca gatcatgatg aacaaacagc gggataaccg
    51721 gcaacggcaa ccctacggtc atgtaggtga gaaaaaccgc aaaagagatg cggaagagcg
    51781 aaaaactggc agaagatcgt gtttcggttt ggcttacagc agtcatgcat tactccagaa
    51841 tgcagcgcaa ggcgaggagt atccccgtct catctctctg gtttcagggt tacggtgcgt
    51901 tggcaggatt taacgcgtac gtcttttcag aaggaaatcg acaaagcggg aagtttgcct
    51961 agaactggtg gcgattgtca atgatgtgaa aaagggaacc atcaggttcc cttttgcgtt
    52021 agtgccggag gccgcattgg cgcgtaacgt cggatgcgac gctggcgcgt cttatccgac
    52081 ctactccggc ctactgcgaa ttaatctttt cagcttgcgc attaccagcg tggcgttggt
    52141 gccgccgaag ccgaagctgt tagacataac agtggtcagt tcgcgatcgg tcgtttcggt
    52201 cacgatgttc agacccgcag cctgctcgtc cagctcttca atgttgatgc tcggggcgat
    52261 aaagccgtgt tccagcatca gcagagagta gatagcttcc tgcacacccg cagcgcccag
    52321 tgagtgaccg gtcatggctt tggttgcaga aatcgccggg ctcttatcgc cgaacacttc
    52381 acggatagct gccagctctt tcacgtcgcc aaccggagtc gaagtaccgt gagagttcag
    52441 gtaatcgatt ggggtatcaa cgccatgcat cgccatctgc atacaacgta ctgcgccttc
    52501 gccagacgga gcaaccatgt ctgcaccatc agaggttgcg ccgtagccaa cgatttcagc
    52561 atagatgtaa gcaccacgcg ccagcgcgtg ttccagctct tcaaccacta ccataccgcc
    52621 gccgccagcg ataacgaaac cgtcacggtg agcgtcgtaa gtacgggagg ctttttccgg
    52681 ggtgtcgttg tatttagtag atagcgcacc catcgcgtcg aattcgcagg ccatttccca
    52741 gcacagctct tcgccgccgc cagcaaacac gatgtcctgt ttgcccagtt ggatctgctc
    52801 tactgcgtta ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg agtagttaac
    52861 gccatgaatt ttaaacggcg tggcgagaca ggcagaaacg ccggatgcca tcgctttagt
    52921 gaccacatac gggccaaccg ctttcaggcc gcgcgggccg cgcattgcgt cagcgccgaa
    52981 cacctggaaa cgcggggaac cgccgccgga acctgcaatc aggccaacgc gcgggttatt
    53041 ctggtaagct tccggagaga ggcccgcatc agcgattgcc tgctccatag aaaggaatgc
    53101 ataaatggat gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc cagtggtatc
    53161 cagttttatg ttgccccaga cgtggctacg catgccggaa tccttcagct cctgagagaa
    53221 agtgatccct gaacgtcctt cacgcagaga tgccaggact tcctgctggt tattaccgat
    53281 gctggaaaca atgcccaggc cagtaatcac tgcacgtttc attcaatacc tctgtaagtc
    53341 gcacatagag taagtttcga atgcacaata gcgtacactt gtacgccgaa caagtccgat
    53401 cagccattta atagagatat ttgcgcagcc ttacacacat cgctaagatc gagccaccgc
    53461 ctgtaagacg agtaacttac gtgaaacact actccataca acctgccaac ctcgaattta
    53521 atgctgaggg tacacctgtt tcccgagatt ttgacgatgt ctatttttcc aacgataacg
    53581 ggctggaaga gacgcgttat gtttttctgg gaggtaacca attagaggta cgctttcctg
    53641 agcatccaca tcctctgttt gtgatagcag agagcggctt cggcaccgga ttaaacttcc
    53701 tgacgctatg gaaggcattt gatcagtttc gcgaagcgca tccgcaagcg caattacaac
    53761 gcttacattt cattagtttt gagaaatttc ccctcacccg tgcggattta gccttagcgc
    53821 atcaacactg gccggaactg gctccgtggg cagaacaact tcaggcgcag tggccaatgc
    53881 ccttgcccgg ttgccatcgt ttattgctcg atgaaagccg cgtgacgctg gatttatggt
    53941 ttggcgatat taacgaactg accagccaac tggacgattc gctaaatcaa aaagtagatg
    54001 cctggtttct ggacggcttt gcgccagcta aaaacccgga tatgtggacg caaaatctgt
    54061 ttaacgccat ggcaaggttg gcgcgtccag gcggcacgct ggcgacattt acgtctgccg
    54121 gttttgtccg ccgcggtttg caggaagccg gattcacgat gcaaaaacgt aagggctttg
    54181 ggcgcaaacg ggaaatgctt tgtggggtga tggaacagac attaccactc ccctgctcca
    54241 cgccgtggtt taatcgtacg ggcagcagca aacgggaagt agcgattatc ggcggtggta
    54301 ttgccagcgc gttgttgtcg ctggcgttat tacggcgcgg ctggcaggta gcgctttatt
    54361 gcgcggatga agcccccgcg ctgggtgctt ccggcaatcg ccagggggcg ctgtatccgt
    54421 tattaagcaa acacgatgag gcgctaaacc gctttttctc taatgcgttt acttttgctc
    54481 gtcggtttta cgaccaatta cccgttaaat ttgatcatga ctggtgcggc gtcacgcagt
    54541 taggctggga tgagaaaagc cagcataaaa tcgcacagat gttgtcaatg gatttacccg
    54601 cagaactggc tgtagccgtt gaggcaaatg cggttgaaca aattacgggc gttgcgacaa
    54661 attgcagcgg cattacttat ccgcaaggtg gttggctgtg cccagcagaa ctgacccgta
    54721 atgtgctgga actggcgcaa cagcagggtt tgcagattta ttatcaatat cagttacaga
    54781 atttatcccg taaggatgac tgttggttgt tgaattttgc aggagatcag caagcaacac
    54841 acagcgtagt ggtactggcg aacgggcatc aaatcagccg tttcagccaa acgtcgactc
    54901 tcccggtgta ttcggttgcc gggcaggtca gccatattcc gacaacgccg gaactggcag
    54961 agctgaagca ggtgctgtgc tatgacggtt atctcacgcc acaaaatccg gcgaatcaac
    55021 atcattgtat tggtgccagt tatcatcgcg gcagcgaaga tacggcgtac agtgaggacg
    55081 atcagcagca gaatcgccag cggttgattg attgtttccc gcaggcacag tgggcaaaag
    55141 aggttgatgt cagtgataaa gaggcgcgct gcggtgtgcg ttgtgccacc cgcgatcatc
    55201 tgccaatggt aggcaatgtt cccgattatg aggcaacact cgtggaatat gcgtcgttgg
    55261 cggagcaaaa agatgaggcg gtaagcgcgc cggtttttga cgatctcttt atgtttgcgg
    55321 ctttaggttc tcgcggtttg tgttctgccc cgctgtgtgc cgagattctg gcggcgcaga
    55381 tgagcgacga accgattccg atggatgcca gtacgctggc ggcgttaaac ccgaatcggt
    55441 tatgggtgcg gaaattgttg aagggtaaag cggttaaggc ggggtaatct gctctcgcag
    55501 tgtttgccgg atgcggcgtg aacgccttat ccaacctacg tgtgacgtgt aggcctgata
    55561 agacgcgata agcgtcgcat caggcatggt gctccagacg ccgcaactta cagctgtgac
    55621 gcctgctgaa acaggttttc ccacatatcg gtcaccagcg cctggtcacg cggcgacaat
    55681 tcaccggcac caatggcttt ttccagactc tggctaacgg tcgtatgcac cgcctgagcg
    55741 gagtggtcat caccactttc cagttctgcg atggctaatg tcaggtggcc acgcaaatac
    55801 ccactggcaa acaactcatc atcactggca tggtcaacca tgccgtcgat taatgccaga
    55861 atgcgtgatt caaactccgc gatcatcttc tttcctcatt gtaaaacgtg gcgctggctt
    55921 cagttgagcg cttccggcca cgggaactgt tccgccgtaa gttccggcgt gtgataataa
    55981 ttttgtagtg ctttaatgaa gcgtgccggg cgttccggaa tgccatttgt cagataatcc
    56041 atcacctgcg catgtacccg ccgttgaaac accacacgat ccggttcgaa atccccttcc
    56101 agattgtcgc aactgacgtt aaacggatat ccagccgcca cgcagaataa ccagtcgaac
    56161 gcctgcggct tcacttcaac atcttcaaac tggctttgcg tctgggcatc gcgtccgtcc
    56221 gggcaatacc agtagccgaa atcaaccagc tcacggcgcg cttttccggc aatacaccag
    56281 tgcgaaatct catgaattgc gctggcataa aagccatgag caaagacgat tcggttatac
    56341 ggtacttccg catcagcagg aagatagatc ggttcgtcgt cgcctttaat cagacgggta
    56401 ttaaattcat cggcaaagca gctattaaaa atttcaatca actgctcgta gtggtgtgta
    56461 ctgttcatta gttcatcccc aaccagtgga ggatctcctg tccgtggcta tcgtaaagta
    56521 atttggcact catcaccgcc gagacgataa caatcatcgg gcggatcagc ttttgtcctt
    56581 tgctcaacac caaccgtgaa cccatgcgcg cgccgaggaa ctgccccacc agcatgacaa
    56641 agcccgtcgc ccaaatcact ttgccgccga gaataaacag cagcagaccg ccaatgtttg
    56701 acgttgcgtt gagtaatttg gcgtgagccg tggctttggc gaggttgaat ccgcacagca
    56761 taacgaaggc cagcgcgtaa aacgatccgg cagccgggcc aaagaatccg tcataaaaac
    56821 cgacgcagcc accggcaatt aacgcgaagg gtaaaccgta catccggcgc tggcgatctt
    56881 cttcacccag ctttggcatc agcaagaaat agaggccgat acaaatcacc agaatgggca
    56941 aaatctgccg taagacatca gcctgaacgt actgtaccaa cagtgcgccg ctcattgagc
    57001 cgacaaaggt catggcaata ttgagtttct gatcgcttaa actaaccact ttgcggcgaa
    57061 taaagtagat agtggcggaa atagagccgc cgcaggcttg cagtttattg gttgccagcg
    57121 cgttagcggg agacattccc gcagccatca atgccggaat ggtgagtaac ccaccgccac
    57181 cggcaatcga gtcgataaat cccgccagca tggcgacaaa aaagagaact cccagcaaca
    57241 gcggagaaac cataaacagg ctattaaacg tttccattag atcacgtgct catccagtag
    57301 cgcctggcag gaaggcggca acggaggcgg tgtcttcttc tcaggctttg ttgttcccgg
    57361 ttttggaggt tcaaaccagc tttgcagttc tgccccgcaa ccatcgcctg atggcggtaa
    57421 aggttgatct tcacactcca gactatcggc agggcaacgt aatcgtacat gcatatgcgc
    57481 gcgatgctgg aaccagggtc gcactttgcg caaccagtcg cgatcggtgc ccgcatcaag
    57541 gcaaagttgt tgtttaatcg ccggattaac aaaaatgcgc gtgacgtctt tgtcctgggc
    57601 ggcgagtttg atcaagctga aaatttctgg cttccacagc gtggagacaa cgtgtttacc
    57661 gtcgcgggaa actaagtcca gtgcttgcgg gcgcaagagc tgcgcggagg tccagcgagt
    57721 tttcggcagt tgcagaaaga tatcgacatc cagtccggtc tggtggctgg catgaccgcc
    57781 gttgaaacgc ccaccagcgg gcatccccat atcgccaatc agcaccgtac ccatgcccag
    57841 attgctcacc tggctactca gacgctggat aaacatcacc agatccgggt gaccgaaata
    57901 gcgacgctga tcggtacgca tgacctgata atgttcggac tgtatcggca acgtgtcagc
    57961 gccgacaata cagccattag aaaaactgcc tatcgattgt gcgctacccg gcacaggttg
    58021 ggttattttt tgccacggcg tcgctgccag gctagcacta ctggcaagca gagccagcag
    58081 cgcaatcgcg gttttgttca ttttttacca gcgtggaata tcagtcttca catcggcatt
    58141 ttgcgcccgc tgccgtaaca ggtgatccat taaaacgatc gccagcatcg cttcagcgat
    58201 cggcactgca cggatcccga cacagggatc gtgacggcct ttggtgatca tctcaacttc
    58261 ttcgccaaag cggttaatag tgcgccccgg cacggtaatg ctggaggtcg gtttcagcgc
    58321 catatgagca atgatttgct gcccgctgct gataccgccg aaaatgccgc ccgcatggtt
    58381 gctctggaaa ccgtctttgg tgatttcgtc gcggttctgg ctgccgcgca gcgccactac
    58441 gtcaaaaccg tcgccaattt ccacgccttt caccgcgttg atgctcatca gcgcatgggc
    58501 gatgtcggca tccaggcggt caaagaccgg ctcgccaagt ccggcgggga caccactggc
    58561 aacaacggtg actttcgcgc cgatggagtc gccctctttt ttcagcgcgc gcatcagttc
    58621 atctaacgcg tcgattttgt cggggtccgg gcagaagaac ggattttgtt caaccagcga
    58681 ccagtctttg atttccagcg gaatgtcgcc catctgggtc aggcagccgc ggatttcaat
    58741 accaaatttc tcggcgagat attttttggc aatcgcccct gccgccacgc gcatggcggt
    58801 ttcgcgggcg gaagaacgtc caccgccgcg ataatcgcgc agaccgtatt tttgttcgta
    58861 ggtgtaatca gcatggcctg gacggaagac gtctttaatt gcgctgtaat cctgagaacg
    58921 ctggtcggta ttttcgatca acaagccaat gctggtgcca gtagtaacgc cttcaaaaac
    58981 accggagagt attttgacct gatccggctc gcggcgctgg gtggtatagc gcgatgtccc
    59041 agggcgacga cgatcaaggt catgttgcag gtccgcttcc gtcagcggaa tgcctggcgg
    59101 aacaccatcg acgatgcagc cgagcgccag cccgtgcgat tcgccgaagg tggttacgcg
    59161 aaagagttgt ccaattgtgt ttccagccat cacggctccg ttattgttgt gtttgcgtgt
    59221 ttacttaatc tttataaatc gcgaaatgtt ctcgtgcggc aataagctgc tctttagtga
    59281 gcataaacac accatcgccg ccgttatcaa actccagcca ggtgaacgga acatcgggat
    59341 attgttccat aagatgtacc atgctgttgc ccacttcaca aatcaacacg ccatcatcag
    59401 caaggtaatc tgccgcgtta ccgagaatgc gacgcgtcag tttcaggccg tcggtgccag
    59461 atgccaggcc cagttccggc tcgtgacggt attcgtttgg caggtcggac atatcttccg
    59521 cgtcgacata cggcgggtta gtgacaatca gatcgtattg cactttcggc aaatcacgga
    59581 acagatcgga acgaatcgga atgacgttgt ggatcagacc gtgttcttcg atgttctgtt
    59641 cggcaaccgc cagcgcgtcg ggagagatat ctaccgcgtc gacttccgct tccgggaagg
    59701 cataagcaca ggcaatggcg atgcagccgc taccggtaca catatctaaa atatgctgcg
    59761 gttgcttgct gatgagtccg gcaaatttat tgttgatcag ttcaccaatc ggcgagcgcg
    59821 gcaccagcac gcgttcatcg acgtaaaatt catgaccgca gaaccaggct ttgttggtca
    59881 ggtaagccac cggaatgcgc tcgttgacgc ggcggatcac gcgttcaaca atacggtgtt
    59941 tttcgctgga ggtcagacgc gcggtgcgca tatcttccgg gatatccagc ggcaggtaga
    60001 gcgatggtaa caccagctgt acggcttcat cccacgggtt atcggtgccg tgaccgtacc
    60061 agatatttgc cgcgctgaag cggcttaccg accagcgcaa tatgtcctga atggtttgca
    60121 gctcatttac tgcttcatca acgaaaattt tatccacgta ttcctccagg gcatgatcgc
    60181 aataatttcg gcggctagtt tgccatgaag atgacgataa atcagcattc acgcgcggtg
    60241 agtgaggaaa aatacgttta aaacgatcga ttgtgctacg agtcgggtaa actgtaggaa
    60301 aattagaaat agagacagat aaatgaaaaa gaaaacaaca cttagcgagg aggaccaggc
    60361 tctgtttcgc cagttgatgg cggggactcg caagattaag caggacacga ttgtccaccg
    60421 accgcagcgt aaaaaaatca gcgaagtgcc ggtgaaacgc ttgatccagg agcaggctga
    60481 tgccagccat tatttctccg atgagtttca gccgttatta aataccgaag gtccggtgaa
    60541 atatgttcgc ccggatgtca gccattttga ggcgaagaaa ctgcgccgcg gcgattattc
    60601 accggaattg tttttggatt tacacggtct gacgcagctg caggccaagc aggaactggg
    60661 cgcgttgatt gccgcctgtc gccgcgaaca tgtgttttgt gcctgcgtga tgcatgggca
    60721 cgggaagcat attttgaagc aacaaacacc gctgtggctg gcacaacatc cgcatgtgat
    60781 ggcatttcac caggcaccga aagagtacgg cggtgatgcg gctttgttgg tgttgattga
    60841 agtggaagag tggttgccgc cggagttgcc ctgaatgctg gcagcaaatg ccggagcagc
    60901 gtaaacgcct tatccggcct acaaatatgt gataattcaa taaattgcaa ttcacttcgt
    60961 aggcctgata agacgcatca gagcaatact acgaacttca gttcattcaa tacatactga
    61021 aaacgacacg tcgaatcaat cggttgccca ctactggtat tcacattcac cacaatatcg
    61081 tgtagtactt tatccgttac cggatgacga gttggcgtaa ccgttaccgt tgatttatca
    61141 caaccgtaag aggtaaaggt taacaattct gataccgcgc ggcagtatgc tcctctgcca
    61201 tcattatcag aaaaattact gccatcacag agtctgccgc cttccatcag gtaggtcgaa
    61261 agcgtggtgg tcactgttga ccccgtggac tgaattcgta acgccttatt atggctgaca
    61321 taattttcac tccaggagat atcgtatgaa gcgaccggta tcgttgggca tattgtgtta
    61381 tgcactgtag gggccgtata gcttccaaat ccgggtaaat ctgtatagga ataaaaggat
    61441 gcgatccaag ggcaaccata atcatcagat gcaacggtaa ttttaaacgt catatatgca
    61501 caacgaaaaa ttttcgtatc tatgccaggg caatttgagt tattaattgt atcaaagtca
    61561 gtcagtacca aagttggagc tgaacccgac ccagaccagg aaataacccg caacgtatcg
    61621 cctttatacg caaaagaatc atatttccca tatgcaacct cctttggcgt tatcaccgta
    61681 acattgaaaa cactgctcgc tacagcaccg gttaccttgt ccatggcata ataaactctc
    61741 acactgtcaa aactcgcact cataagttta tctccagcat aactcttaga tgcgccgaca
    61801 acactcagaa cgagcagcat taggctcgcg aaaattatgg tccatttttt catttcagaa
    61861 atactcaaag gttgccgtca ctgtggcact aaagtctccg gacgttacat ctcgcccgct
    61921 ctttgcctgt aaccaggtat taaaatttag gctgttattt ccctgattca gcgggagcgt
    61981 gacgcctgtt gtattgttga ttggcaccga tgtgcctgcc gcagtttcaa taccaatccc
    62041 agcgccggat gctgatgacg aggagtcaaa ggccagaaag ccgggtagag cactatcttc
    62101 tgttcctgtc aacgttactt tgacattaaa caatgtagaa ctatgacaat ctttcaattg
    62161 aaacaccaat ggcactcgct ctgactgccc aaggttcatt aaatcactgg cggcaatggc
    62221 aggaaaatgt acttctgcca actggctacc attaatcact ggcgtacacg atttactgat
    62281 taaattgccg gtaaagtgca aattatctcc tgctattgtg gttccactgc ccagcaacat
    62341 caggctacta ataaaaaaca acgtacgctt tttcttcatt ggtaatctac ctgtaacgtg
    62401 gcccaggcct caaagtccgc ctctggcaga atgacaccac tcttttttac tggcacagcc
    62461 gttaacaccg gtaaatcggt ttcattcacc acaagtggcg tattgatcgt aaaactctgc
    62521 cctgcctgtt tgagctgaat gcctaaacca gtgatattgc tgcttactgc agcattatca
    62581 aaatcgcttg gtgtgccact ccagctcaag gtcatttcca ttaatgggtc gcgaaccgcc
    62641 gtatcgcagg taatggaata cggcacggtt tgctcataat tgctgccatt aatgtcatca
    62701 ataagcacat cctgaaaagt tatttcaatg gtttgagcgt tgctaatcgt gcagttaggc
    62761 ggctgcacta aataaccgtg aaaagagacg tttgagagtg ccatcgagca aaagccccac
    62821 agcaaaataa gcgagatccg tttcattggt aatccaccac aattgtggca ctggcgttaa
    62881 attcagctgc cgctaacgtt gtaccactct gtttcaccgg cacagcttcc agaacgggaa
    62941 ctcccgagga aaaattaaat ggcaaccatg agccagaggt taagtcgagt attgtgtgat
    63001 cactactttt ctgaacccga ataccaaacc caggaatacc cgtgctgata actgtttcac
    63061 cgttaatcac gaccgttgtc gcctgcatct gtagacgcag gtcatccatt gccagattgt
    63121 tacaaacaag cgagtagtca atgggacgtt tataatcaac cctattgatc ttattaatga
    63181 atacattacc aaactcaact gccgcaccat ttaccgtaca aggaggaggg gcatcgacaa
    63241 gcacccgtaa attcatactt actgacttac tcgctgcaac tgcaaagccg ctaccaacac
    63301 aacatatcag taacccagtt accgcccaga cagaacaaga tcctttcata ctggctctcc
    63361 cggcctcagc ctttgcggct ctgctcttca tcgacctagc aactgttacc gttgcaagtg
    63421 aaaaagagcg gcatccgagc gccatagtcg ttgacatagg tcagtaccgg cacgctaccc
    63481 atcttcacat tcagcggtgc agaggttttc ggcgacatca ctattggcga gaaaccggct
    63541 gccggattgc catttttctg tgtgctggca ttactgaaaa tgacgtaata cgacgtcggg
    63601 ttattgacgg taaattcctg accgttgcgg gttaaagtaa tcttatattg ctaggggttt
    63661 ttcatgctga cgttctccag cgccttcggt cgccagaaca acttaatacg cgtttgtagt
    63721 gctatttgca gcgtgttggc tttgtttgat tttggcggga tctcacgcac gttgaagtag
    63781 aacaggcttt cacgatcggc aggcaatttg ttgatatccg gcattccctg cacttttacc
    63841 tgcccgttca tcattgaatc gatgcgttgc accggtggca gaacagttaa cggtgaactg
    63901 atcttgttac ccttttcatc ttcgatccag ctttgggcca gataaggcag tttcggatcg
    63961 ttattgcgca acgtcacgct gattgatttg tcgctttcat taaatatcaa acgggtacga
    64021 tctggcgtta cagaagcctg ggcagacaaa ctggcagcgc taagcagtaa cgctagcgtc
    64081 gtagcaccca atttggcaga gcaaagtaaa tcagacatgg atcattcctt aatcacgtta
    64141 ttggttagct gaaacagaaa cagggggatt ggtgggtgtc acctgttgcg tctgctcctg
    64201 aatgacaggc tttatgtcat caggaacaac cggcgcgacg ttgcctttat gctggcatgg
    64261 cagtaacaag ccgttaaaca gatcggcagg cagcgggtcc ggcaggttga tatcgcaatg
    64321 cgcaacacca ctccagaaca cactcatgtg ttcgccaggt ttcactccag ccagataaac
    64381 attgccgtca tcatcgacaa ggcccactgt ctgctcgtta tcatttttta cttctgcgcc
    64441 aaacggagga tggctgccgt cttgtaaacg cagcaccgcc atcgcttttt gaccactgat
    64501 gacggcaaat ttgcggtagc caatcgcccc ttcagttagc gtggcttgta ccaccgactg
    64561 ggttgcttcg gcgttttccg gcagtttgtt gaggtcgata tacgcctgat tgcggtaata
    64621 gttattgacg tcagaaacga cggctttacc aaacatattg gtataaacag cagcaccgtt
    64681 accttctacc ggaacatcgg caacgccatc ggcatcaatc aacaagcgtg tcccgcccat
    64741 attctgggta cggtgaagtg cgccaccgtg ggcagtaagc gttgcgccgc cctgtaacga
    64801 caagcccgca gaggtgtatt gcccttcatg gtagttcgca ctgaggtcaa cctgcgccag
    64861 cgaaccatca tggctgtaat agccgtcaac gctggtgtgt ttgtcactgg tgccgacgtt
    64921 caactgatag tgagtcgcgt catcgacacg gctgaaataa ccgacctgac tgctgtccgt
    64981 cccactgcca tagtaccgtt atagctaacg gtactgttgt cgccccacgg catactgagc
    65041 gaaatgtaca tgcctttgtc ggcctggttg tcatactcgt aacggtagcc agtcatcgag
    65101 atgctgacat tacgaatact gcccatattg aaatagtgcg agagcatgat gttgtagttt
    65161 gtctgctcct cgcgatccca gtaggtatgg cgggtatagt tgagataaac cgaaacaccc
    65221 gcatcgcgga agttctggtt ataagtggcg gtgtacatct ctttgtcgtt gcccgtgcgt
    65281 accattccgc tgtcactggc atccagatac tcgctcatgg tcataaagtt ctcttccgag
    65341 aagcgatatc cagcaaaagt aacgcggctg ttgagctggt caaagtcttt ggaataactc
    65401 acacggaagg agttaccgtc cagcgaacct ttgccataag cggtgtcttt atccagtttg
    65461 gtgtgcgagt gagtaacatc aaacgcgacc gcgccgaatg tagacaaatc gcgaccgaca
    65521 ccaagcgccg cagactgata gttttcatct cccagtgcgc cgccatataa cgaccagccg
    65581 ttagcgatcc cccaggaagc ttcagcacca gaaaaaaatt caccctcgac atggtgtccc
    65641 cactcttgcg gacggcccat catgatctta tagcgaacct gacctggacg agtgaggtat
    65701 ggcatcgagg cggtgctgat gtcatattcc tgcacctggc cgttctgttc ttcaatgcga
    65761 atatgcaacg taccggagac agaatcacca agatcctgaa tacgaaacgg tccagccgga
    65821 acctgcgttt cgtaaatcac acgccccatc tggctgacgg tcacttttgc tgtggtgtgt
    65881 gccacgccgg aaatgtctgg cgcgtagccg cgcagattgg gaggcaacat ttgatcgtca
    65941 gtactgacac tgccaccaac atagttaaaa ccatcaaaaa tatcggatct gaggtaatcc
    66001 tcgcccagcg ccagtttggc ttttaatgat ggtaacgccc gcctggcata gtagcgactc
    66061 cactcccatt tttttgagtt tcatcgccgc tgaattcatc gtcatcatta ctgcgagtat
    66121 gttgatagtt agtctgccag tcagcacgca tacgccacgg ccctaggtta accccaaccg
    66181 tcccgttgcc gctgatctcg ttactatcat caccgccatt ttcttcgtgc cgggtttgtg
    66241 cgttgatgct gtagtccgcg acgatcccgg agatgccgtc atcccaacgt gaaggcggat
    66301 ccctgtcggg ataagtatat tcgaggtaag cctgtggcag tgaaatgact aatgcggact
    66361 ggcttaaatc agccttaatt tccatgcctt ccagttgacc ggatttcagg catttagcat
    66421 cgtggctcca ttgcagattt ttcgccacgt cttcttttaa accaaactgc gctaccagtt
    66481 ccggtgtcag acaagcatag cttttgctcg cgtcatcttc accagcatac cagtaaatat
    66541 cgtactcttc cgccaatggc tgtttattta gttgaacctg taaattgtat tttccgggct
    66601 caacatatcc ctggctggaa aaacgcttca tatcaatttt tgtgtcgcct tttaattcca
    66661 gaaaacggga atcaaactga atgtcgtctt cagcccagac actgctataa ctccctgaca
    66721 ttgccagcgc aatgcaccac ggaagaatcc gtaatcgaaa aagagaatgg tcaggcatac
    66781 acgtttctat ccgtttttat tcttgttcgg tcaaaataca tttaaatccc ccatagcagt
    66841 gtcgctatgg gggtatgcct tacaggtaag taatctggaa ggtggtattt gcttcaaaat
    66901 tacccagatc tggcgcatca gctgcgccga ccagccaggc gttaaagttc agtgtctgct
    66961 tcgctttgcc tttgttggta gaagtgtctt tcactatttt ctggtcaata cccatgccgc
    67021 ttttgtaaga ggtacccaga gagtcaccga tcgccaggct aacattgtta aatgccgcac
    67081 cggtatcggt attgaaaatg gtgtaataat tgccgctatt tgcagaagaa acggtaccag
    67141 taaaggtagt ggtcattgtt tcctgagtat caaatacgca atcttgcaga cgaatctgga
    67201 aacttttcgg cgtggtggtg ccgttatttt tcagtttttc ggcaccgata tcacccatat
    67261 caacttccag tttgtgatcg tccggaacga tagaacacac tgagtcttca atggtgccgt
    67321 aaaaacgtgc agtaccgttg ttacccgcag cgattgttgc cgtagaggtg aacacgccca
    67381 tcaccattgc cgcagcaata gctgttttaa caaacttact cattatttgt ccttaaacta
    67441 taaataatta aaaatataaa cattgcaata cattgattca gtcaatagcc aatgtttata
    67501 cccagccatt gaatacggca tgcgacccaa aaagaacagg tcgtagaaaa ttgtattcat
    67561 tggtgtgggg ccgcatagtg atgtttccac acccctgatc caatttaatt ttactaaaga
    67621 gtaaatgtag atttaattaa tatattgaca gaagaaaggt tatcttaagg a
//
LOCUS       DADLTY010000003        52717 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.003, whole genome shotgun sequence.
ACCESSION   DADLTY010000003 DADLTY010000000
VERSION     DADLTY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.003"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..326
                     /locus_tag="JN334_000162"
     CDS             <1..326
                     /locus_tag="JN334_000162"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="catalyzes the interconversion of bicarbonate and
                     carbon dioxide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY7436439.1"
                     /translation="GLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYN
                     LGHSTIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLK
                     HANHK"
     gene            complement(367..903)
                     /gene="hpt"
                     /locus_tag="JN334_000163"
     CDS             complement(367..903)
                     /gene="hpt"
                     /locus_tag="JN334_000163"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAY7436440.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            1109..3499
                     /locus_tag="JN334_000164"
     CDS             1109..3499
                     /locus_tag="JN334_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HAY7436441.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(3546..5096)
                     /gene="cueO"
                     /locus_tag="JN334_000165"
     CDS             complement(3546..5096)
                     /gene="cueO"
                     /locus_tag="JN334_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HAY7436442.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            5262..5609
                     /locus_tag="JN334_000166"
     CDS             5262..5609
                     /locus_tag="JN334_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HAY7436443.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            5715..6581
                     /gene="speE"
                     /locus_tag="JN334_000167"
     CDS             5715..6581
                     /gene="speE"
                     /locus_tag="JN334_000167"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAY7436444.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            6597..7391
                     /gene="speD"
                     /locus_tag="JN334_000168"
     CDS             6597..7391
                     /gene="speD"
                     /locus_tag="JN334_000168"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAY7436445.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(7429..7791)
                     /locus_tag="JN334_000169"
     CDS             complement(7429..7791)
                     /locus_tag="JN334_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HAY7436446.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(7966..10563)
                     /gene="acnB"
                     /locus_tag="JN334_000170"
     CDS             complement(7966..10563)
                     /gene="acnB"
                     /locus_tag="JN334_000170"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HAY7436447.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            10918..12771
                     /locus_tag="JN334_000171"
                     /pseudo
     CDS             10918..12771
                     /locus_tag="JN334_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(12842..14266)
                     /gene="lpdA"
                     /locus_tag="JN334_000172"
     CDS             complement(12842..14266)
                     /gene="lpdA"
                     /locus_tag="JN334_000172"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAY7436448.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(14591..16471)
                     /gene="aceF"
                     /locus_tag="JN334_000173"
     CDS             complement(14591..16471)
                     /gene="aceF"
                     /locus_tag="JN334_000173"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAY7436449.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(16486..19149)
                     /gene="aceE"
                     /locus_tag="JN334_000174"
     CDS             complement(16486..19149)
                     /gene="aceE"
                     /locus_tag="JN334_000174"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAY7436450.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(19310..20074)
                     /gene="pdhR"
                     /locus_tag="JN334_000175"
     CDS             complement(19310..20074)
                     /gene="pdhR"
                     /locus_tag="JN334_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HAY7436451.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            20615..21988
                     /gene="aroP"
                     /locus_tag="JN334_000176"
     CDS             20615..21988
                     /gene="aroP"
                     /locus_tag="JN334_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAY7436452.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(22031..22885)
                     /gene="ampE"
                     /locus_tag="JN334_000177"
     CDS             complement(22031..22885)
                     /gene="ampE"
                     /locus_tag="JN334_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HAY7436453.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(22882..23433)
                     /gene="ampD"
                     /locus_tag="JN334_000178"
     CDS             complement(22882..23433)
                     /gene="ampD"
                     /locus_tag="JN334_000178"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HAY7436454.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            23521..24414
                     /gene="nadC"
                     /locus_tag="JN334_000179"
     CDS             23521..24414
                     /gene="nadC"
                     /locus_tag="JN334_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAY7436455.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            24617..25057
                     /gene="ppdD"
                     /locus_tag="JN334_000180"
     CDS             24617..25057
                     /gene="ppdD"
                     /locus_tag="JN334_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HAY7436456.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            25067..26214
                     /gene="cpaF"
                     /locus_tag="JN334_000181"
                     /pseudo
     CDS             25067..26214
                     /gene="cpaF"
                     /locus_tag="JN334_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
     gene            26204..27406
                     /gene="hofC"
                     /locus_tag="JN334_000182"
     CDS             26204..27406
                     /gene="hofC"
                     /locus_tag="JN334_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HAY7436457.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(27441..28484)
                     /gene="guaC"
                     /locus_tag="JN334_000183"
     CDS             complement(27441..28484)
                     /gene="guaC"
                     /locus_tag="JN334_000183"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAY7436458.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            28709..29329
                     /gene="coaE"
                     /locus_tag="JN334_000184"
     CDS             28709..29329
                     /gene="coaE"
                     /locus_tag="JN334_000184"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAY7436459.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            29329..30072
                     /gene="zapD"
                     /locus_tag="JN334_000185"
     CDS             29329..30072
                     /gene="zapD"
                     /locus_tag="JN334_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAY7436460.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            30082..30279
                     /gene="yacG"
                     /locus_tag="JN334_000186"
     CDS             30082..30279
                     /gene="yacG"
                     /locus_tag="JN334_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAY7436461.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(30379..30768)
                     /gene="mutT"
                     /locus_tag="JN334_000187"
     CDS             complement(30379..30768)
                     /gene="mutT"
                     /locus_tag="JN334_000187"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAY7436462.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(30828..33533)
                     /gene="secA"
                     /locus_tag="JN334_000188"
     CDS             complement(30828..33533)
                     /gene="secA"
                     /locus_tag="JN334_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAY7436463.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(33595..34107)
                     /gene="secM"
                     /locus_tag="JN334_000189"
     CDS             complement(33595..34107)
                     /gene="secM"
                     /locus_tag="JN334_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HAY7436464.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(34338..35255)
                     /gene="lpxC"
                     /locus_tag="JN334_000190"
     CDS             complement(34338..35255)
                     /gene="lpxC"
                     /locus_tag="JN334_000190"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAY7436465.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(35356..36507)
                     /gene="ftsZ"
                     /locus_tag="JN334_000191"
     CDS             complement(35356..36507)
                     /gene="ftsZ"
                     /locus_tag="JN334_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAY7436466.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(36568..37830)
                     /gene="ftsA"
                     /locus_tag="JN334_000192"
     CDS             complement(36568..37830)
                     /gene="ftsA"
                     /locus_tag="JN334_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAY7436467.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(37827..38657)
                     /gene="ftsQ"
                     /locus_tag="JN334_000193"
     CDS             complement(37827..38657)
                     /gene="ftsQ"
                     /locus_tag="JN334_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAY7436468.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(38659..39579)
                     /locus_tag="JN334_000194"
     CDS             complement(38659..39579)
                     /locus_tag="JN334_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase B"
                     /protein_id="HAY7436469.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(39572..41047)
                     /gene="murC"
                     /locus_tag="JN334_000195"
     CDS             complement(39572..41047)
                     /gene="murC"
                     /locus_tag="JN334_000195"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAY7436470.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(41101..42168)
                     /gene="murG"
                     /locus_tag="JN334_000196"
     CDS             complement(41101..42168)
                     /gene="murG"
                     /locus_tag="JN334_000196"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAY7436471.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(42165..43409)
                     /gene="ftsW"
                     /locus_tag="JN334_000197"
     CDS             complement(42165..43409)
                     /gene="ftsW"
                     /locus_tag="JN334_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HAY7436472.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(43409..44725)
                     /gene="murD"
                     /locus_tag="JN334_000198"
     CDS             complement(43409..44725)
                     /gene="murD"
                     /locus_tag="JN334_000198"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAY7436473.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(44728..45810)
                     /gene="mraY"
                     /locus_tag="JN334_000199"
     CDS             complement(44728..45810)
                     /gene="mraY"
                     /locus_tag="JN334_000199"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAY7436474.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(45804..47162)
                     /gene="murF"
                     /locus_tag="JN334_000200"
     CDS             complement(45804..47162)
                     /gene="murF"
                     /locus_tag="JN334_000200"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAY7436475.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(47159..48646)
                     /gene="murE"
                     /locus_tag="JN334_000201"
     CDS             complement(47159..48646)
                     /gene="murE"
                     /locus_tag="JN334_000201"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAY7436476.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(48633..50399)
                     /locus_tag="JN334_000202"
     CDS             complement(48633..50399)
                     /locus_tag="JN334_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HAY7436477.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(50415..50780)
                     /gene="ftsL"
                     /locus_tag="JN334_000203"
     CDS             complement(50415..50780)
                     /gene="ftsL"
                     /locus_tag="JN334_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAY7436478.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(50777..51718)
                     /gene="rsmH"
                     /locus_tag="JN334_000204"
     CDS             complement(50777..51718)
                     /gene="rsmH"
                     /locus_tag="JN334_000204"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAY7436479.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(51720..52178)
                     /gene="mraZ"
                     /locus_tag="JN334_000205"
     CDS             complement(51720..52178)
                     /gene="mraZ"
                     /locus_tag="JN334_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAY7436480.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        12466 a        14727 c        13028 g        12496 t
ORIGIN      
        1 tggggcttat caacaactgg ctgctgcata tccgcgatat ctggttcaaa catagctcat
       61 tgctcggcga aatgccgcaa gagcgccgtc tggatacctt gtgtgaactg aacgtcatgg
      121 aacaggtgta taacctgggc cactccacca ttatgcaatc agcgtggaaa cgcgggcaga
      181 aagttaccat tcacggctgg gcctacggca ttcacgacgg cttgctgcgt gatctggatg
      241 ttaccgccac caaccgcgaa acccttgagc aacgttaccg tcacgggatt tccaacctca
      301 agctgaaaca cgccaaccac aaataaaaat gccatgccgg atgcaacaca tccggcaact
      361 tcacacttac tcgtccagca gaatcacttt gccaatatac ggcagatgac ggtaacgctg
      421 tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg atcgagaaac cgataaattc
      481 aaccgggacg ttcacttcac gacgggacgg tttatccagc agcgtacaaa tcgccaacga
      541 cttcggttcg cgcaggctta agatctcacg cactttcgac agtgtattcc ccgagtcgat
      601 gatatcttca acaatcagca cgtccttgcc acggatatct tcatccagat ctttgaggat
      661 tttcacatca cgggtggtgg acatgccgct accgtagctg gaggcggtca taaagtcgac
      721 ttcatgagat acctgaactt cacggcacag gtccgccata aacataaatg agccacgcag
      781 cagacccacc agcaccatat cgctgccgct gtctttgtaa cgctcagtaa tctgacgacc
      841 cagttcggcg atacgcgctt taatctccgc ttcggggatc attacttcta cagtatgttt
      901 catatctcta accatatgat ttaaaaacaa atcacttcgc acgggcaggt tttggcaccg
      961 caacaaagcg caagacgcgc agtataccag taaaactatt cattcgcgga atctgttaat
     1021 aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct ataattaatt gctactaaaa
     1081 tattaatgaa ttgaaatggt gtctctttat ggcaattaac aatacagcct cgcgacgatt
     1141 actcgtcacg ctaacagccc tttttgcagc gctttgcggg ctgtatctac tcattggcgg
     1201 aggctggctg gtcgcgattg gcggctcctg gtactaccct atcgctggcc ttgtgatgct
     1261 cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg ctttggctat acgcagccct
     1321 gctgctcggc accatgattt ggggcgtctg ggaagttggt ttcgacttct gggcgctgac
     1381 tccgcgcagc gatattctgg tcttcttcgg catctggctg atcctgccgt ttgtctggcg
     1441 tcgcctggtc attcctgcca gcggcgcagt tgccgcactg gtggtcgcac tgctgattag
     1501 cggtggtatc ctgacctggg ccggatttaa cgatccgcag gagatcaacg gcaccttaag
     1561 cgccgatgcc acacctgccg aagctatctc ccccgttgcc gatcaggact ggcctgcgta
     1621 tggccgcaat caggaaggtc aacgcttttc accactgaag caaattcacg ccgataacgt
     1681 ccacaagctg aaagaagcct gggtgttccg tactggcgat gtgaagcagc cgaacgatcc
     1741 gggtgaaatc accaatgaag tgacgccgat taaagtgggc gatacccttt acctgtgtac
     1801 cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc aaagagaaat ggcattacga
     1861 tcctgagctg aaaaccaacg agtctttcca gcacgtaacc tgccgtggcg tctcttatca
     1921 tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg gattgcccgc gtcgtatcat
     1981 tcttccagtg aatgatggtc gcctgattgc gattaacgct gaaaacggca aactgtgcga
     2041 aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat atgccagaca ccaaaccggg
     2101 tctgtatgag ccgacttctc cgccaattat caccgataaa accatcgtga tggccggttc
     2161 agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg atccgtggtt ttgatgtcaa
     2221 caccggggag ctgctgtggg cttttgatcc gggcgcgaaa gatccgaacg caatcccgtc
     2281 agacgaacac acctttacct ttaactcacc aaactcgtgg gcaccagcgg cctatgacgc
     2341 gaagctggat ctggtctatc tgccgatggg cgtgaccacg ccggatatct ggggcggtaa
     2401 ccgcacaccg gaacaggaac gttatgccag ctcgattctg gcgctgaatg ccactaccgg
     2461 gaaactggcg tggagctacc agaccgttca ccacgacctg tgggacatgg atcttccggc
     2521 acagccgacg ctggcggaca tcaccgttaa tggtcagaaa gtgccgatta tttacgctcc
     2581 ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat ggcgaactgg tggttccggc
     2641 cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat tacgttaccc ctactcaacc
     2701 attctcggaa ctgagcttcc gtccgacgaa agatttgagc ggtgcggata tgtggggagc
     2761 caccatgttt gaccaactgg tgtgccgcgt gatgttccac cagatgcgct atgaaggcat
     2821 tttcacgccg ccatctgaac agggtacgct ggtcttcccg ggtaacctgg ggatgttcga
     2881 atggggcggg atttccgttg atccaaatcg tgaagtggca attgccaacc caatggcact
     2941 gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac ccgatggagc agccgaaaga
     3001 tgccaaaggc acgggtacgg aatccggcat tcagccacag tacggtgtac cgtatggtgt
     3061 cacgctcaac ccgttcctct caccatttgg tctgccatgt aaacagccag catggggtta
     3121 tatctcggcg ctggatctga aaactaatga agtggtgtgg aagaaacgta ttggtacgcc
     3181 gcaggacagt atgccgttcc cgatgccggt tccggtgccg ttcaatatgg gtatgccgat
     3241 gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt atcgccgcta cggcagataa
     3301 ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg tggcagggtc gtttaccggc
     3361 gggtggtcag gcaacaccga tgacctatga agtgaatggc aagcagtacg tggtgatctc
     3421 cgcaggcggt cacggttcat ttggtacgaa gatgggcgac tatattgtgg cttatgccct
     3481 gccggatgat gtgaaataac cataatgccc ggagagatcc gggcatattt ccgaatacgg
     3541 tctttttata ccgtaaaccc taacatcatc cccgtatctt catgctccag cagatggcag
     3601 tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa acttcaccag cacttcactg
     3661 acattgcctt ctaccttaac ggtatctttc cagcccgcgc gatgagccgc tggcggtttg
     3721 ccattttctg acaagatacg gaactgcgtg ccgtgaatat ggaacggatg cagcatcatg
     3781 tcgcccacgc cagagataac ccaacgttcg tactgccctt tcgccgccgc aaacatcggc
     3841 ttattcatat caaacgcctg accgttgatt ttattggcat ggtggaaatc gaacttcccg
     3901 ccgtggttca tatgattcat attgccgtgc cccatatggc ccatcatctg gctgtgatcc
     3961 attccggcca tcgcctgatc gccatatttc tccattagca tctgcatccc catcatatcg
     4021 agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca gcccttccag cgaaggtaac
     4081 gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg cactaatagc aatcggctga
     4141 atccgcatta ccggatgcgg cttgtcaaac ggtgcaatcg ccatccccat ctggctgacc
     4201 ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct ccaccagcac ttcaaaacgc
     4261 tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt caggtagcag accaccgtcg
     4321 ctggcaatca catacagcgg gcgattgtcg ctggtggcga aattgagcga gcgggcatta
     4381 cagccattga gcaaacgcag gcgcagccaa ccacgcgggg cagcgtgttg tgggtagatt
     4441 gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg cggcggtcat cacatccagt
     4501 tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct gaacgatcac cggaacatca
     4561 tcgatacccc actgttttgg cagcattaat ttcaggatct cgtcatcttc aatcaccacc
     4621 agaccagcca gccccatcgc cacctgtcgc ccggtcttgc catgttgatg tggatggaac
     4681 cagcaggtag cggcaggttg atcaacgttc aacgtcaccg agcgcttgcc acctggcgga
     4741 ataattccct gtgggccgcc gtcgacgtca cccggtactt ccagcccgtg ccagtgcaac
     4801 gtcgtctctt ccgccagttg gttgtagata tcaaccgtta ccgctttgcc gcgctgtaat
     4861 ttcaccgccg gccccagcag attgccgtta tagccccagg tagttgcagt tttctcgcca
     4921 aaggtggact gacctgcgcc aatagttaac tgaatgcgat tacgggcatc ggtcgtgagc
     4981 aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga ctgcacggct ccacagtggc
     5041 aaggctgaag ccacacccag tgcgacggaa tatttcaaga aatcacgacg ttgcatagtt
     5101 atttccttat tcttaagcag gcgaaacaaa atcaaacgtt gagcatagtc cttcccctta
     5161 cgggaaggtc aagccgcaga cacaatttaa tcgacaataa tggtcgtcgc ctcgctcgca
     5221 gtgtgctaac gtttatcttc tttaagcccc tggtagaagc aatgaagacg tttttcagaa
     5281 cagtgttatt cggcagcctg atcgccgttt gcgcaaacag ttacgcgctc agcgagtctg
     5341 aagccgaaga tatggccgat ttaacggcag tttttgtctt tctgaagaac gattgtggtt
     5401 accagaactt atctaacggg caaattcgtc gcgcactggt ctttttcgcc cagcaaaacc
     5461 agtgggatct cagtaattac gacaccttcg acatgaaagc tctcggtgaa gacagctacc
     5521 gcgatctcag cggcattggc attcccgtcg ctaaaaaatg caaagccctg gcccgcgatt
     5581 ccttaagcct gcttgcctac gtcaaataat ccctgatacc tttttgtaga aatattgacc
     5641 gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt tttttacggg tgttaacaaa
     5701 ggaggtatca acccatggcc gaaaaaaaac agtggcatga aacgctacac gaccagtttg
     5761 ggcagtactt tgcggtagat aacgttctgt atcatgaaaa gaccgatcac caggatctga
     5821 tcatttttga gaacgctgca tttggtcgcg taatggcgct ggatggcgta gtacaaacca
     5881 ccgagcgcga cgagtttatc tatcatgaga tgatgaccca tgttccgcta ctggcccacg
     5941 gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg tgccatgctg cgtgaagtaa
     6001 cccgacataa aaacgttgag tcaatcacga tggtggaaat cgatgcgggt gtcgtgtcgt
     6061 tctgccgtca gtatctgccc aaccataacg ccggtagcta cgacgatccg cgctttcaac
     6121 tagtgatcga cgatggcgtc aatttcgtta atcaaaccag ccagaccttt gatgtcatta
     6181 tctccgactg caccgatcct atcggtcccg gcgaaagcct gttcacctca gcattttatg
     6241 aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt cgcacaaaac ggcgtctgct
     6301 ttttacagca ggaagaagcc atcgacagcc atcgcaaact cagccattac ttcagcgacg
     6361 ttggctttta tcaggcggcg atcccgacct attacggcgg tatcatgact tttgcatggg
     6421 cgacagataa cgacgcctta cgccatctct caaccgaaat tattcaggcg cgttttctcg
     6481 cttctggcct gaaatgccgt tattacaatc cggcaatcca tacggcagct tttgccttac
     6541 ctcagtatct gcaagacgca ctggcttcac agccgtccta aggagaagat aagaaattga
     6601 aaaaactgaa actgcatggc tttaataatc tgaccaaaag tctgagtttt tgtatttacg
     6661 atatctgcta cgccaaaact gccgaagagc gcgacggtta tattgcttat atcgatgaac
     6721 tctataatgc caaccgtctg accgaaatcc tgtcagaaac ctgttccatt atcggggcca
     6781 atattcttaa catcgcccgc caggattacg aaccacaggg tgccagcgtc actattctgg
     6841 tgagtgaaga accggttgac ccgaaactca tcgacaaaac agaacacccc ggcccactgc
     6901 cagaaacggt ggttgctcat ctcgataaaa gtcatatttg cgtacatacc tacccggaaa
     6961 gtcatcctga aggcggttta tgtaccttcc gcgccgatat tgaagtctct acctgcggcg
     7021 tgatttctcc gctgaaggcg ctgaattacc tgatccacca gcttgagtcc gatatcgtaa
     7081 ccattgatta tcgcgtgcgc ggttttactc gcgacattaa cggtatgaag cactttatcg
     7141 accatgagat taattcgatt cagaacttta tgtctgacga tatgaaggcg ctgtatgaca
     7201 tggtggatgt aaacgtctat caggaaaata tcttccatac caagatgttg cttaaagagt
     7261 tcgaccttaa gcactacatg ttccacacca aaccggaaga tttaaccgac agcgagcgcc
     7321 aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat ttattacggg cgcaatatgc
     7381 cagctgttta acggctctgg cggagctccc aggctccgcc agatttattt acttctgctg
     7441 cacgaaattg cggtaagccg ccacgacctg cagaaaatcc tcaacgccgc atagcgacag
     7501 gctttcttcg tcgtagtagt tcatcccctc ttccatttca tcgccagcga attccagttg
     7561 attggcgcga accatcacct cttcagcgtc catccacagg gtgtattcat gccctgcccg
     7621 ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct tgttccactt catcaagcaa
     7681 ggccaggttt tctttcacct cttcattaaa ccagtgcccg accacttcat gccccatgga
     7741 catacgcacc tttaccactc cggtaatatc gcgcagaaat tcgtaatcca tagtgtgttc
     7801 ctctgctccc ggcaatgcgc cgaaatcgca tcgcgccatt gatgtaatta tcgcagcctt
     7861 accggagaaa aacagcggaa cggcgatagt ttagaaataa aaaatgcccg ggaatctcat
     7921 tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt acttattaca ccgcagtctg
     7981 gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc tggttgaagt tcagataacg
     8041 ataagtatca acggcagttt tatctacttg cgcaacgtag gtctggtact cttccggcgt
     8101 cggcagtttg ccaatcagcg ccgcaacagc cgccagttct gcagaagcca ggaagacatt
     8161 cgcgccagta cccaaacggt tcgggaagtt acgggtagag gtggaaacca ccgtcgcacc
     8221 gtctgctaca cgcgcctggt tacccataca cagggaacag cccgggatct cgatacgagc
     8281 cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc agttgcgcgg cgtccatacg
     8341 ggttggcggt gccacccaca ggcgggtcgg caactgacct ttgtgcgcat ccagcagttt
     8401 accagcggca cggaagtgac cgatgttagt catgcaagaa ccgataaata cttcgtcgat
     8461 cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc ggatcgttcg gagcacacag
     8521 gattggctct ttaatctccg ccagatcgat gtcgatcact gccgcgtatt ccgcatctgc
     8581 atcggcttcc agcagctcag gattcgccag ccatttttcc atgccctgaa tacggcgttc
     8641 cagggtacga cgatcgccgt aaccttccgc gatcatccac ttcagcagga cgatgttgga
     8701 gctcaggtat tcgatgatcg gttctttgtt cagcttgata gtacaaccag cggcagaacg
     8761 ctcggcggac gcatcggtta gctcaaaggc ctgctcaact ttcagatccg gcagaccttc
     8821 aatttccaga atgcgaccag agaagatgtt tttcttgcct ttcttctcaa cggtcagcag
     8881 accttgtttg atcgcataca gcggaatggc gtgtaccaga tcgcgcaggg tgatgcccgg
     8941 ctgcattttg cctttgaagc gcaccagaac ggattccggc atatccagcg gcattacgcc
     9001 agttgcggca gcaaacgcca ccagaccaga acccgccggg aaagagatac cgatcgggaa
     9061 acgggtatgg gagtcaccac cggtaccgac ggtatccggc agcagcatac ggttcagcca
     9121 ggagtgaatg acgccgtcac ccggacgcag cgacacaccg ccacggttca taatgaagtc
     9181 cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga tacgccgcgg tgtggcagaa
     9241 agactgcatc accaggtcag ccgagaagcc caggcacgcc aggtctttca gttcatcacg
     9301 ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc attttcggtt cgcagtacgc
     9361 gcccggacga atgcctttca cgccacaggc acggcctacc attttctgcg ccagcgagaa
     9421 gccgcgatcg ctctcagcga catctttcgc ctgacggaac acatcactgt gcggcagacc
     9481 aagtgcttca cgcgctttgg tggtcaggcc acgcccgata atcagcggaa tacggccgcc
     9541 agcacgcact tcatcaatca gcacgtcggt tttcagttcg aaggtcgcca gcagttcgcc
     9601 tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg tcaatcacgt cgcccatgtt
     9661 caggttagag acgttgactt cgattggcag tgcacccgcg tcttccatcg tgttaaagaa
     9721 gatgggtgca attttaccgc cgaggcacaa accaccgccg cgtttgttcg gcacatgtgg
     9781 aatatcatcg cccataaacc acagaacgga gttcgtggcg gatttacgcg atgaacccgt
     9841 acccacaacg tcaccgacgt acgccagcgg gaaacctttc tgttgcagag cttcaatctg
     9901 tttgatcggg ccaacaacac ctggctggtc tggctcaata ccttcacggg cgtttttcag
     9961 catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac gcatccggtg ccggagaaag
    10021 gtcatcggtg ttggtttcgc cagtgacttt aaagacggta acggtcagtt tttcagccag
    10081 cgccgggcga ttcaggaacc attcggcatc cgcccaggac tgcattacct gcttcgcata
    10141 ttcgttgcct gctttcgctt tctcttctac gtcatagaag ttatcgaaca tcagcagcgt
    10201 gtgagaaagt gctttggcag caataggtgc cagtttggca tcatccagcg cgtcgatcag
    10261 cggatgaatg ttgtaaccac cctgcatggt gcccagcagt tcgatagctt tttccggagt
    10321 cagcagaggg gatttagctt cgcctttagc gatagcagcc aggaagcctg cttttacata
    10381 ggcggcttca tcgacgcctg ggggaacacg gttggttaac agatctaaca ggaattcttc
    10441 ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg gccatttggt ttgcatccag
    10501 gggtttgggc gcaatcccct cagcggcacg ctcagctacg tgcttacggt attcttctag
    10561 cacgacggtt ctcctcgctc tcattgtcat agtgcggcag gcgattctct tcacgctcct
    10621 gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa cgggcagaat agcaggattt
    10681 tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa aagtggctga atcgttaagg
    10741 atggtctaga gattgtttcc ctccgtaaaa acttcgttaa ttcgcatggt gataatcacc
    10801 tttctcaacg aatcccaaga ttagtcaaaa tttaaactac cgcctcttta tactcggatt
    10861 cacagcacct gcgggtggca gttcgccgac cattgcgatt tccttgagat ccgaattatg
    10921 aaaatgactt tgccgtttaa accccatgtg ctggcgctaa tttgcagtgc cgggctttgt
    10981 gctgcctctg ccgggctata tataaaaagc cgcacagtgg aagcgcctgt agaaccgcaa
    11041 tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc ttcctgcaac ggtttccgca
    11101 cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca ctgcacaaat agatcaatgg
    11161 gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc aggtgctgat ggcatcaacc
    11221 tatccgacaa acgttgctca agcagtgcaa tggtcgcacg ataatccact taaacaaggc
    11281 gatgctgcta ttcaggcggt atctgaccag ccgtgggacg ccagcgttaa atcactggtg
    11341 gcctttccac aattgatggc attgatgggc gaaaacccgc aatgggtgca aaacctgggc
    11401 gatgcttttc tggcgcagcc gcaggacgtg atggactcgg tacagcgatt gcggcaactg
    11461 gcgcaacaaa ccggctcgct gaagtcatca accgaacaga aagttattac cacaacgaag
    11521 aaagctgtac cggtaaaaca gacagtcacg gcacccgtca taccatccaa taccgtttta
    11581 actgccaacc ccgtcattac agagccggca acaaccgtca tttccattga gcccgccaat
    11641 cctgatgtgg tctatattcc caactacaac ccaaccgtgg tttacgggaa ctgggccaat
    11701 actgcgtatc cgccggttta tctgccacca ccagccggag aaccgtttgt tgacagcttt
    11761 gtgcgcggat tcggctatag catgggcgtt gctaccacgt acgcactatt cagcagcatc
    11821 gactgagatg acgacgatca tgaccatcat catcatgacg atgatgatta tcatcaccac
    11881 gatggcggtc atcgtgacag taatggctgg caacacaacg gcgacaacat caatatcgac
    11941 gtcaacaatt tcaaccgtat caccggtgag catcttactg ataagaatat ggcatggcgg
    12001 cacaatccaa actaccgtaa tggtgtgccc tatcatgatc aggatatggc aaagcggttt
    12061 catcaaaccg atgtcaacgg cggaatgagt gccacgcagc tacctgctcc aacacgcgac
    12121 agccagcgtc aggcggcagc aagtcagttt cagcaacgaa cacacgccgc ccccgtcatt
    12181 acacgagata cccaacgtca ggcagcggca cagcggttta atgaagctga acactatggg
    12241 agctatgacg acttccgcga cttcagccgt cgccaaccac tgacccagca gcaaaaggac
    12301 gccgctcgtc agcgttatca gtcggcctct cctgagcagc gccaggcagt ccgcgagaaa
    12361 atgcagacta acccgcagat ccagcagcga agggaggcgg cgcgccagcg tattcagtcc
    12421 gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc agactaaccc gcagaaccag
    12481 cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa ctcccgaaca gcgccaggtg
    12541 tttaaggaaa aagtacagca gcgcccactg aaccaacagc aacgtgataa cgcccgccag
    12601 cgtatacaat cggcatcacc tgaacaacgt caggtttttc gggagaaagt tcaggagagc
    12661 cgcccacaac gtctaaacga cagtaaccat actgtcaggc tgaataacga gcaacggtca
    12721 gcagtacgcg aacgtctctc tgaacgtgga gcaagaagac tggaaaggta aattgcagac
    12781 gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg gatgttccgg caaacgaaaa
    12841 attacttctt cttcgctttc gggttcggca ggtcggtaat gctaccttcg aacacttctg
    12901 ccgccaggcc cacagactcg tgcagagtcg ggtgcgcgtg gatggtcagt gcgatgtctt
    12961 cagcatcaca acccatttcg attgccaggc cgatttcacc cagcagctcg ccgccgttgg
    13021 taccaacaat cgcaccaccg atcacacggt gagattcttt gtcgaaaatc agcttggtca
    13081 taccgtctgc gcagtcagaa gcgatagcac gaccagaagc agcccacggg aaggtggcgg
    13141 tttcatagct gatgcctttc tctttcgctt ctttctcagt cagacctacc catgcaactt
    13201 ctggttcggt ataggcgatg gacgggataa ctttcggatc gaagtagtgt ttcttaccgg
    13261 cgataacttc agcggcaacg tgaccttcgt gaacaccttt gtgtgccagc atcggctgac
    13321 cgacgatatc gccgatagca aagatgtgcg gtacgttggt acgcagctgt ttgtcaacgc
    13381 ggatgaaacc acggtcgtca acttccacgc cagctttgcc tgcgtcgagg tttttaccgt
    13441 tcggcacacg accaatcgct accagcacgg cgtcgtaacg ctgcggttca gctggtgctt
    13501 ttttgccttc catcgtcaca taaataccgt cttctttcgc ttcaacggcg gtaactttgg
    13561 tttccagcat caggttgaat ttcttgctga tacgcttggt gaagacttta acgatgtctt
    13621 tgtcagctgc cgggataacc tggtcgaaca tttcaaccac gtcaatctgt gaacccagcg
    13681 cgtggtaaac ggtgcccatt tccagaccga tgataccgcc acccattacc agcaggcgtt
    13741 ctggtacttc tttcagttcc agcgcgtcag tggagtccca gatacgcgga tcttcatgcg
    13801 gaataaacgg cagttggatc gggcgagaac ccgctgcaat gatcgcattg tcgaagttga
    13861 tcacggtttt gccgttctca ccttcaactt ccagggtgtt agccccggtg aatttaccca
    13921 gaccgttgac cactttgact ttgcggcctt tcgccatacc agccagacca ccggtcagct
    13981 gattgatcac tttctctttc caggtacgaa tcttgtcaat atcggttttc ggttcgccga
    14041 agacgatacc gtgttcagcc agcgctttgg cttcttcgat aacttttgct acgtgcagca
    14101 gtgctttaga agggatacag ccgacgttca ggcaaacacc gccaagggtg ttgtaacgtt
    14161 ctacgattac ggtttccaga cctaaatcag cgcaacggaa ggcagcggag taacctgcgg
    14221 ggcctgcccc aagtaccacg acctgagttt tgatttcagt actcatcatg acctctatat
    14281 atttatctcc ggcggtcata cccgtcgtct ttcaggccgc agcggcgtta gcttcacgcc
    14341 ctcaccccag tcacttactt atgtaagctc ctggggattc tggcgcttgc taccttgctg
    14401 cacccagaaa tccatagggt atgtgaagta ataccctaac caccaccggg tcgttctatc
    14461 cgtcggtatt ttacaaaatt gttaacaatt tttaaacaac aaacggcaac cgatttgtct
    14521 attcgctaat aacctcaata cattcatgag attaccagaa aaaagccggc cgttgggccg
    14581 gctcttttac ttacatcacc agacggcgaa tgtcagacag cgtgttgtta atgatggtaa
    14641 tgaaacgggc accatcagca ccgtcgatca cgcggtggtc gaaggagaga gaaatcggca
    14701 gcatcagacg cggcacgaac tctttaccat tccacaccgg ctccatcgcg gacttggaaa
    14761 cgccgaggat agccacttcc ggcgcgttca caatcggcgc gaagtgggta gtacccaggc
    14821 cgccgatgct ggagatggtg aagcaaccgc cctgcatttc gcccgcagtc agcttaccgt
    14881 cacgcgcttt cttagaaata gtcatcagct cgcgagacag ctcgatgatg cctttcttgt
    14941 tgacgtcttt gaataccgga acaaccagac cgttcggggt atccaccgcc acaccgatgt
    15001 tgatgtattt cttcagggtc agacgctgac cgtcttccga cagcgaacta ttgaagcgag
    15061 gcatctgctc aagagctgca gcaacggctt tcatgatgaa gacaaccggg gtgatcttca
    15121 catccagctt acgtttcgcc gcttcttcgt tctgctgttt acggaacgct tccagctcgg
    15181 tgatatcggt tttgtcgaag tgggtaacat gcgggatcat cacccagtta cggctcaggt
    15241 tagcaccaga aattttctgg atacggccca gttccacttc ttcgatttcg ccaaacttgc
    15301 tgaagtccac cttcggccac ggcagcatgc cagggatacc accgccagtc gccgccggag
    15361 ctgcttctgc acgtttgata gcttctttca cgtaagcctg aacgtcttcg cgcaggatac
    15421 gacctttacg gccagtgccc ttcactttcg ccaggttaac gccaaactcg cgtgccagac
    15481 ggcggatcag cggggtcgcg tgaacgtaag cgtcgttttc agcaaattca gatttgcctt
    15541 ccgctttcgc agccggagct tcagcttttg ctgccggagc cggcgctgcc gcttcctgtt
    15601 tcgcaggagc tgccgcaggc gctgcgcctt caacttcgaa gatcataatc agcgaaccag
    15661 ttttcacttt atcgccaacg ttgactttca gttccttcac gacgcctgca aacggagccg
    15721 gaacttccat agaagcttta tcgccttcta cggtaatcag tgactgttca gcggcaactt
    15781 tgtcgcccac tttcaccatc acttcggtca cttcaacttc gtcaccgccg atatccggaa
    15841 cgttaacttc tttcacgcca gctgctggtg caggggtcgc tgccggagtc gcttcctgtt
    15901 tagccgccgg agctgccgcg cctgcttcac ccgcgacttc gaagaccata atcagcgagc
    15961 cggtagacac tttgtcaccc acgttcactt tgatctcttt cacggtacct gcaaacggag
    16021 ccggaacttc catagaagcc ttgtcgcctt ctacggtgat cagcgactgt tcagcttcaa
    16081 ctttatcgcc aactttcacc aggatttcgg tcacttcaac ttcgtcgctg ccgatatccg
    16141 gaacgttaac gtcttttgcc gccgcagccg cgggtgctgc tgccggagct gcttctttct
    16201 tctcttctgc ctgagcaggt gcagcgtctg ctgcaccgtc ggcggaatcg aaaatcataa
    16261 tcagtgcgcc ggtctgggtt ttatcgccaa cagagacttt gatctcttta acgatacccg
    16321 cctgcggaga tggaacttcc atagaggctt tgtcgccttc tacggtgatc agcgactgtt
    16381 cggcttcaac tttgtcgccc actttgacca ggatctcggt gatttcaact tcatcagccc
    16441 cgatgtccgg tactttgatt tcgatagcca ttattctttt acctcttacg ccagacgcgg
    16501 gttaacttta tctgcatcga tgttgaattt ggcgattgcg tcagcaacca ctttcttatc
    16561 gatttcgcca cgtttagcca gttcgcccag cgccgcaacc acgacataag aagcatcaac
    16621 ttcgaagtgg tgacgcaggt tctcacggct gtcggaacga ccgaagccat cagtacccag
    16681 tacgcgatag tcgtcagccg gtacgtaagt acggacctgc tcagcgaaca gtttcatata
    16741 gtcggtagat gccactgccg gcgcgtcgtt catcacctga gcgatatacg gaacgcgcgg
    16801 agtttccagc ggatgcagca tgttccagcg ttcacaatcc tgaccatcac gcgccagctc
    16861 ggtgaaggag gtcacgctat aaacgtcaga acctacgccg taatctttcg ccaggatctc
    16921 tgccgcttca cggacgtgac gcaggatgga accggagccc agcagctgaa ctttaccttt
    16981 gctaccttca atggtttcga gtttgtagat acctttacgg ataccttcct cagcaccttc
    17041 cggcattgcc ggcatgtggt agttttcgtt cagcgtagtg atgtagtagt aaacgttctc
    17101 ttgtttttca ccgtacatac gctccagacc gtcatgcata atgacagcaa cttcgtaagc
    17161 gtaagccggg tcgtaagaga tacagttcgg gatagtcagc gactgaatgt ggctgtgacc
    17221 atcttcgtgc tgcagacctt caccgttcag ggtggtacga ccggaagtac cgccgatcag
    17281 gaagccacgc gcttgctggt ctccagccgc ccagcacaga tcgccgatac gctggaagcc
    17341 gaacatcgag taatagatgt agaatgggat catcggcaga ttgttcgtgc tgtaagaggt
    17401 cgccgctgcc agccaggaac accctgcgcc cagctcgttg atcccttcct gcagaatctg
    17461 acctttctcg tcttctttat agtaagcaac ctgctcgcgg tcctgcgggg tgtactgctg
    17521 accgttcggg ctgtaaatac caatctgacg gaacagacct tccataccga aagtacgcgc
    17581 ttcgtcggcg atgatcggta ccagacgatc tttgatcgac ttgttcttca gcatcacgtt
    17641 cagagcacga acgaaagcga tagtggtaga gagctctttg ctctgctctt ccagcagcgc
    17701 gccgaagtct tgcaggctcg gcagctcaag cttctcggtg aagttcggct gacggcttgg
    17761 cagataaccg tgcagtttct gacgctgagc gtgcagatag gtatgctctt cagaaccttc
    17821 cgggaaggtg atgtacggca gtttttcgat atctgcatca gacaccggca cattgaaacg
    17881 gtcgcggatg tgacgcacgc cgtccatgtt cattttctta acctggtgcg cgatgttttt
    17941 accttcagcc gcgtcgccca tgccgtaacc tttaatggta tgagcaagga ttactgtcgc
    18001 tttgcctttg gtttcctgcg ctttcttgaa tgcagcgtag attttcttcg gatcgtgacc
    18061 accacggttc agtgcccaga tctgctcgtc agtccagtct gcaaccagtg ctgcggtttc
    18121 aggatattta ccgaagaagt gttcacgaac gtacgcacca tctttcgatt tgaaggtctg
    18181 gtagtcgccg tcaacggttt cgttcatcag ctggatcagt ttaccgctgg tatctttacg
    18241 cagcagttca tcccaacggc taccccacat cactttgatc acgttccagc cagcaccttc
    18301 gaagatgcct tccagttcgt tgatgatctt gccgttaccg gtgaccgggc cgtcaagacg
    18361 ctgcaggtta cagttgataa cgaaaaccag gttatccagt ttttcacggg tagcgatggt
    18421 gatcgcacct ttggattccg gttcgtccat ttcaccgtcg ccgaggaacg cgtaaacggt
    18481 ctgtttagag gtatctttca ggccacggtg ttccagatat ttcaggaatt tagcctggta
    18541 aatagcacca atcggaccca gacccataga tacggtcggg aactgccaga attccggcat
    18601 cagtttcggg tgcggatagg aagagaggcc attgccgtga acttcctgac ggaagttatc
    18661 cagctgctcc tgagtcagac gaccttccag gaaagcacgg gcgtaaacgc ccggggagat
    18721 gtggccctgg aagtaaacca ggtcgccgcc atcctgctcg ttgcgtgcac ggaagaagtg
    18781 gttaaagcac acatcataaa tggttgcgga agactggaag gacgccatgt ggccgcccag
    18841 ttcgaggtct tttttcgacg cacgcagcac cgtcatgatg gcgttccagc ggatagctga
    18901 acgaatacgg cgttccagtt ccagattacc cggatactcc ggttgttctt caacggggat
    18961 ggtgttgatg tagttgctga tacctgtgcc tgcggctacg tttacaccgc ctttgcgggc
    19021 ttcagcaagc agttggtcga tcagatactg agcacgctca acaccttctt cacggatgac
    19081 cgattcgatc gcctggagcc agtcgcgagt ttcgatcgga tccacgtcat ttgggaaacg
    19141 ttctgacatg ggttattcct tatctatcta ataacgttga gttttctgga acctgtccca
    19201 ttgaactctc gccagaaagc tcaataagac aggttctacg tttagttacc gcgcttttat
    19261 atgcgcttga tttacaacat cttctggata atttttacca gaaaaatcac taattctttc
    19321 gttgctccag acgacgcaga gaacgctcac ggcggctctc ttcacggctt ctgtcgagca
    19381 aaatttcttc gataaaggcc agatggcgat gcgatgcttc gcgcgcttct tccggcttac
    19441 cgaccataat cgcttcaaat atgcgggtgc ggtgactact caccagcggc agcatctcgc
    19501 gacgcgaata gagcaattcg aagttctggc ggacattctg ggccaacatc ggctccatac
    19561 accttagcag atgaagcaga accacattgt gggccgcttc ggtgacggca atctgatact
    19621 ggagtacggc gtttgattcc gcgtccagat cgccagactg ctgcgccagc tctatggcgt
    19681 ggtggagttc acggatgcgt tccttgtctt catcggtact acgcagcgcg gcgtaataag
    19741 cggcgatacc ttccagggcg tgtcgtgttt cgagcaagtc atactgtgat tcaggatggt
    19801 cggagagcag ctccaccagc ggatcgctga agctttgcca taggctgctc tggacaaaag
    19861 tgccgccacc ctggcgacga agcaacaagc ccttcgcttc gagacgttga atcgcctcac
    19921 gcaaggaggg acgggagacg tcgaactgtt ttgccagttc gcgttccggt gggagttttt
    19981 cgcccgggcg gagagtgcct tcgaggatca aaaactccag ttgctgctca atcacatcgg
    20041 agagttttgg ttggcggatt ttgctgtagg ccatgagttc ctgtcttaag ccacttgccg
    20101 aagtcaattg gtcttaccaa tttcatgtct gtgacgctaa agtaacaaag tattcacctt
    20161 atgtccatac aggttttgat tgaaatcatg aaactgtgca cattttaaca acttgacata
    20221 tataacgttt caaagttgta actatgcaca aatgtagact ttacgtagga aagggatttt
    20281 ttaacgatta ttaaactata aaaatccgaa ttgaaccgat tcacttacca attttttgat
    20341 ttttaagtca ataaaaacga atttaaattc actctgcata ttgagagggg ttgaggctga
    20401 gctttacaaa cggtttcttt ttaagcaact catcttcaac catgcataaa gcgggtgcat
    20461 tcgctgccgc ataccattat tcttgatctg acggaagtct ttttgtaaca attcaaacct
    20521 ccttgatgta aacaaatcaa tacaacaaac ggaattgcaa acttacacac gcatcactgc
    20581 gtagatcaaa aaaacaatca ccgcacgagg tttcatgatg gaaggtcaac agcacggcga
    20641 gcagctaaag cgcggcctta aaaaccgcca tattcagctt atcgcgctgg gtggcgcgat
    20701 agggacaggg ctattcctag gtagcgcctc cgtaatacag tccgcagggc cagggattat
    20761 cctgggttac gccattgctg gttttatcgc ctttctgatc atgcgtcagc tgggtgaaat
    20821 ggtggtcgaa gaacctgtcg caggctcctt tagccacttt gcttataaat actggggcag
    20881 ttttgccggt ttcgcctctg gctggaacta ctgggtactg tacgttttag ttgccatggc
    20941 agagctgact gccgtgggta aatacattca gttctggtat ccggaaatcc caacctgggt
    21001 ttctgccgcc gtattctttg tggtgattaa cgccatcaac ctgaccaacg ttaaagtgtt
    21061 tggcgagatg gagttctggt ttgccattat caaagttatt gcggtagtag cgatgatcat
    21121 cttcggcggc tggctgctgt tcagtggcaa cggcggcccg caggcgagcg ttagcaacct
    21181 gtgggatcaa ggtggtttcc tgccgcacgg cttcaccgga ctggtgatga tgatggcgat
    21241 tatcatgttc tcgttcggtg gtctggaact ggtggggatc accgcagcag aagctgataa
    21301 cccggagcaa agtatcccga aagcaactaa ccaggttatc taccgcatcc tgattttcta
    21361 tattggttcg ttagccgttc tgctctcact gatgccgtgg acccgcgtta ccgccgatac
    21421 cagtccgttt gtgctgatct tccacgagtt aggcgatacc tttgtggcga atgcgctgaa
    21481 catcgtggta ctgactgcgg cgctctccgt gtacaacagc tgcgtatatt gcaacagccg
    21541 tatgctgttt ggtctggcac aacaggggaa cgcgccaaaa gcgctggcgt ctgtcgataa
    21601 acgcggcgta ccggttaaca ccattctggt gtctgcgctg gttacagcat tgtgcgtatt
    21661 gattaactat cttgctccgg aatccgcatt tggcctgtta atggcactgg tggtatccgc
    21721 actggtgatc aactgggcga tgatcagtct ggcgcatatg aagttccgtc gcgccaagca
    21781 ggaacaaggc gtggtaactc gcttccctgc tctgctttat ccgctgggta actggatctg
    21841 cctgctgttt atggcggcgg tactggtgat tatgctgatg accccaggaa tggcgatttc
    21901 ggtatacctg atcccggtat ggctgatcgt gttaggtatc ggctatctgt ttaaagagaa
    21961 aaccgccaaa gccgtaaaag cgcattaatc tctctacgcc ctcacccgta cagggtgagg
    22021 gcaataatct ctacaccaac gccccgtaaa tcgtcagtag cgcaatcacc accacgacca
    22081 cgaacgaggt tttcttcgcc attgaaaccg ctgccttcgg cgtctccacc ttatcgacat
    22141 gcggttcacg cgccagagag aactgcgcca gacgcgttaa tacctgatat tgcgaagtat
    22201 ggaaatcacc cagcgaagca aaccaggccg gtaacgcttt ctcaccatga ccgatcaagg
    22261 catataccac acccgcaaga cgaaccggca cccaatccag tacatgaagc actgcatcaa
    22321 tgccggactg taaacgatga tgcggcgtct ggtatcgcgc cagccagtat tgccatgcac
    22381 gcaagaacgc ataccccatc agcgtaacgg gtccccaggt tccccccaca atcagccaga
    22441 acagcggtgc aagataaaaa cgaaagttaa tccacagcaa tgcattttgc agctcacgca
    22501 aatactcacg ttcgtcgcag cctgccggga cgccgtgaat catggtgagt tcgccagcca
    22561 tcgtggcacg ggcatggcta tcattacgtg aagcagctgt cagataagca tgataatgaa
    22621 gacgaacttt acctgcgcca atacacagca aaccaatcag cagccacacc agcagcgtgg
    22681 gaacgttgaa caatactccc tgcaatgcgc gtaacagtaa aaaagtcaca cccatcgcaa
    22741 taatggtcat gcctaacgtg cgcccgagag aaaaatgttt cacccgccga aagaacgctt
    22801 caagacgatg atcaagctgc cagtgctcgc ccaacttaaa caggcgctcg aaaattaaca
    22861 ccagtaaggt tgtaaatagc gtcatgttgt ctccttgctg accagcgcac gaaaccgtgc
    22921 ccaatcaaat gcaggaccgg gatcggtttt ccgatccggc gcaatatcac aatggcccgt
    22981 catgttatta gcgatatcgg gatagcgatc tatcagtgcc cgcgtaaccg ccgcaagctg
    23041 ttgatactgc gcatcggtat acgccagagt atcggtgcct tcaagctcaa tcccaataga
    23101 aaaatcattg cagcgttcac gcccctgata ctgagagact cccgcatgcc aggcacgttt
    23161 atcgaaagga acatactgga ctatttcacc atcacggcga atcaaacagt gagcggagac
    23221 gcgcaaatgg gcgatctcag caaagaaagg atgtgcctgc ggatcaatag ttccagtgaa
    23281 taatgcgtcg atccacggac cgccaaactc gcctggcggc aggctaatat tgtgcaccac
    23341 cagcagggtg ggtgtttcgt catccaggcg gcaatcgtaa tgtggtgagg gaacgcggcg
    23401 cgcgccaacc agccacccct gttctaacaa catgcaggat ctccttatat gtggtgctaa
    23461 tacccggttc agagtagcat gtttctacct tatgattcgt tagctatctg gagttttaac
    23521 atgccgcctc gccgctacaa ccctgacacc cgacgtgacg agctgctgga acgcattaat
    23581 ctcgatatcc ccggcgcggt ggcccaggcg ctgcgggaag atttaggcgg aacagtcgat
    23641 gccaacaatg acattacggc aaaactttta ccggaaaatt ctcgctctca tgccacggtg
    23701 atcacccgcg agaatggcgt attttgtggc aaacgctggg ttgaagaggt gtttattcaa
    23761 ctggcaggcg acgatgtcac cataatctgg catgtggatg acggcgatgt catcaacgcc
    23821 aatcaaccct tgttcgaact tgaaggccca tcccgcgtgc tgttaacggg cgaacgcact
    23881 gcgcttaatt ttgtgcaaac cctttcagga gttgccagta aggtacgcca ctatgtcgaa
    23941 ttactggaag gcaccaacac gcagttgttg gatacgcgca aaaccttacc tggcctgcgt
    24001 tcagctctga aatacgcggt actttgcggc ggcggagcga atcaccgtct ggggctttct
    24061 gatgccttcc tgatcaaaga aaaccatatt attgcctccg gctcagtgcg ccaggcggtc
    24121 gaaaaagcgt cctggctgca tccggatgcg ccagtagaag tcgaagtaga gaatctggaa
    24181 gaacttgatg aggccctgaa agccggagcc gatatcatca tgctggataa cttcgaaaca
    24241 gaacagatgc gcgaagccgt caaacgcacc aacggcaagg cgctactgga agtgtctggc
    24301 aacgtcactg acaaaacact gcgcgaattt gccgaaacgg gcgtggactt tatctccgtc
    24361 ggtgcgctaa ctaaacacgt acaagcactc gacctttcaa tgcgttttcg ctaattgctg
    24421 acagatttat ccgctccatc gcgggcggat aatctcccct tccccaactt tcttcgtaac
    24481 gcctcgcaaa tttcttgtct ttcgctgcaa cgaaataaaa aacctcggaa tctgctttcc
    24541 cgtttcgttt tttgcccctc gcctgtgctg atggcgtttg ccaaagtagc accaaccaaa
    24601 tcaaggagcg aaacagatgg acaagcaacg cggttttaca cttatcgaac tgatggtggt
    24661 tattggcatc attgccattt taagctccat tggtattccc gcttatcaaa actacctgcg
    24721 caaagccgca ctcaccgaca tgctacaaac ctttgtgcct taccgtaccg ccgtagagtt
    24781 gtgcgcgctg gaacatggtg gattagatac ctgcgacggt ggcagcaatg gcattccctc
    24841 gcctaccacc acccgctatg tttcagccat gagtgtggca aagggcgtgg tgtcgctgac
    24901 cgggcaagaa agtctcaatg ggctaagcgt cgtcatgacg ccaggttggg ataacgcaaa
    24961 cggcgtcacc ggctgggcgc gcaactgcaa tattcaaagt gacagcgcat tgcagcaagc
    25021 ctgcgaagat gtcttccgct ttgatgacgc caactaagga gcggcaatga atattccaca
    25081 gctcacggcc ctgtgcctgc gttatcaggg agtcttgctg gatgccagcg aagaagtggt
    25141 tcatgttgcg gtggtcgatg ccccctcaca tgagttgctg gacgcattgc atttcgctac
    25201 caccaaacgt attgagatca cctgctggac gcgccaacaa atggaaggtc acgccagtcg
    25261 cacacaacag acattacccg tagctgttca ggagaagcat cagcccaaag cagagttgct
    25321 gactcgaacg ttacaatctg cgctggaaca acgcgcgtct gatattcata tcgaaccagc
    25381 ggacaatgcc taccgcatcc gcttgcgtat cgacggcgta ttgcatcctt taccggatgt
    25441 ttcaccggat gccggagtcg cattaacagc cagattaaaa gtgctgggaa acctggatat
    25501 tgcggaacat cgcctgccgc aggacgggca attcactgtc gaactggcag gaaacaccgt
    25561 ctcatttcgt attgcgacct taccatgtcg gggtggtgaa aaggtggtat taaggttgtt
    25621 acagcaggtg agccaggcac tggatgttaa cacgctggga atgcagccgt tacaactggc
    25681 ggactttgct catgccttgc aacaaccaca gggactggtg ctggtaactg gccctacagg
    25741 cagtggcaaa acggtcacct ggtgttgtcc acgctacaca ccaattccac cactgaaacg
    25801 ctggtacgtt tacagcaaat gggggtcgcc cgctggatgc tatcatcggc gcttacgctg
    25861 gtaatagccc agcgtctggt acgtaaactt tgcccacatt gtcgccgaca gcaaggggag
    25921 cccatccata ttccagacac tgtatggccg tcgccgctgc cccactggca ggcacccggt
    25981 tgtgtacatt gctaccacgg tttttatggt cgtaccgcct tgtttgaagt cctgcccatt
    26041 acaccagcca tacgccagct tatttccgct aatactgaag ttgaatcgct ggaaacgcac
    26101 gcccgacagg cgggtatgcg tacgcttttt gaaaacggct gcctggccgt ggagcaaggc
    26161 ttaaccacct ttgaagagtt aatccgcgta ctggggatgc cgcatggcaa gtaagcaact
    26221 ctggcgctgg catggcataa ccggcgacgg caatgcgcaa gatgggatgc tatgggcaga
    26281 gagccgtact ttactgctta tggcactaca gcaacagatg gttaccccac taagcctgaa
    26341 gcgaatcgcc atcaattctg cgcagtggcg aggagataaa agcgcggaag tcattcatca
    26401 actggcgacg ctacttaaag ccgggttaac gctttctgaa gggctggctc tgctggcgga
    26461 acagcatccc agtaagcaat ggcaagcgtt gctgcaatcg ctggcgcacg atctcgaaca
    26521 gggcattgct ttttccaatg ccttattacc ctggtcagag gcatttccgc cgctctatca
    26581 ggcgatgatc cgcacgggtg aactgaccgg taagctggat gaatgctgct ttgaactggc
    26641 gcgtcagcaa aaatcccagc gtcagttgac cgacaaagtg aaatcagcgt tgcgttatcc
    26701 catcatcatt ttagcgatgg caatcatggt ggttgtggca atgctgcatt ttgttctgcc
    26761 ggagtttgcc gctatctata agaccttcaa caccccacta ccggcactaa cgcaggggat
    26821 catgacgctg gcagacttta gtggcgaatg gggctggctg ctggtgttgt tcggctttct
    26881 gctggcgata gccaataagt tgctgatgcg ccgcccgacc tggcttatcg cgcggcagaa
    26941 attgctgtta cgcatcccga ttatgggttc actgatgagg ggacaaaaac tcacgcagat
    27001 ctttacgatt ctggcgctga cacaaagtgc aggcattact tttttacagg gcgtagagag
    27061 cgtcagagaa acaatgcgct gcccgtactg ggtgcaactt ctgacacaaa tccagcacga
    27121 tatcagtaac ggtcatccca tctggctggc gctaaaaaat gccggtgagt ttagcccgct
    27181 ctgtttgcaa ttagtgagaa caggagaggc atccggctcg ctggacctca tgttagacaa
    27241 cctcgcccat catcatcggg ataacacaat ggcgctggcg gataacctcg cagccttact
    27301 ggaaccggcg ttgctgatca taacgggagg aattatcggt acgctggtgg tggcaatgta
    27361 tctgccaatt ttccatttag gcgatgcgat gagtgggatg ggataacgct ggcgtgttgc
    27421 tccacgccag cgttgggaga ttacaggttg ttgaagatgc ggttttcttg ttccagcaca
    27481 cgaataaacg tggtgcgctt ggtcagctct ttcaggcgtg aagccccaac gtatgtacaa
    27541 gctgaacgca ggccgcccaa aatatcacgc gcggtatttt caacctggcc tcgcagcggc
    27601 agcttaacgg ttttaccttc tgctgcgcgg tattccgcaa cgccgccaac gtgacgtttc
    27661 atcgcagact cggagctcat gccgtagaac agcataaatt tctcgccgtt ctcctcaacg
    27721 atgcgaccgc cgctctcttc gtggcccgcc agcatgccgc caagcatgac gaaatcggca
    27781 ccgccgccaa aggctttcgc cacatcgccc ggcgtggtac agccaccgtc gctgacgatc
    27841 attccgccca gaccgtgcgc cgcatcggca cattcgatta ccgcagaaag ctgcggataa
    27901 ccgacgcctg ttttgacgcg agttgtacaa acagaacctg ggccaatgcc aactttaacg
    27961 atatcagcac cggagaggat aagctcctca cacatttcac cagtcactac gttgccagca
    28021 caaatggttt tggtcggcca cgcttcacgc gctttcgcaa cgaactgcac gaagtgttcg
    28081 gaataaccat tcgccacatc aatacaaacg aagtttaatg ccgggttcag gtcgagaatc
    28141 tgtttagttt tttcgaaatc cgcatcagac gtgccggtag aaaccatcac atgtttcagc
    28201 acatcagcgg aagaattgtt gataaacgct tgccactctt cgacagaaaa gtgtttatgc
    28261 acagcagtca gaatatcaaa agaagccagc gcagaggcca tagaaaatgt gcctacggtg
    28321 tccatatttg cggcgataat cggcacgccg gaccagctct gacctgaatg tttgaaggtg
    28381 aattgacgtt ccagttcaac atcggaacgg cttttaagag tggagcgttt agggcggatg
    28441 agaacgtctt taaaacctaa cttcagatct tcttcaatac gcatgtgcgg attcctgggg
    28501 ttaatggcta taagggtaag agcgcaactc cagtgacgct atcatacgca ctaatcaatg
    28561 cggcgcaaga ctgcgaaatt gtcttttttt acgctacaat cccataaatt tacctggtaa
    28621 atagtgagcg aacgtttcat caatttcact ttcgcgcgct catttttaac gttttgataa
    28681 tcatgatgaa ttcccgggaa tataaattat ggggtatata gttgccttaa cgggaggcat
    28741 tggcagtggt aagagtaccg ttgccaatgc gtttgctgat ctcggaatta acgtcattga
    28801 tgccgatatt attgcgcgtc aggtggttga accaggtgca cctgcgctac atgccattgc
    28861 tgatcacttt ggcgaaaaca tgattgctgc tgatggaaca ttgcagcgcc gggccttgcg
    28921 cgagcggatc ttcgccaacc cggaagagaa aaactggctt aacgacctgc tgcatccgct
    28981 gattcagcaa gagacgcaac accagatcca gcaagcaact tccccctatg tactgtgggt
    29041 tgtgccattg ctggtagaaa actcactgta taaaaaagcg aatcgagtgc tggtggtgga
    29101 tgtcagccca gaaacgcaac ttaagcgcac catgcagcgc gatgatgtaa ctcgcgaaca
    29161 tgtcgaacaa atccttgctg ctcaggcaac gcgcgaagcc cgccttgccg tggcagatga
    29221 cgtcattgat aataacggcg caccggatgc tatcgcatcg gatgttgccc gcctgcacgc
    29281 acactatttg cagcttgcgt cgcagtttgt ctcacaggaa aaaccgtaat gcagacccag
    29341 gtcctttttg aacatccact aaatgaaaaa atgcgtacat ggctgcgcat tgagtttttg
    29401 attcagcaac tcaccgttaa tttacccatc gttgaccacg ctggcgcgct gcatttcttc
    29461 cgtaatgtca gtgaattact ggatgttttc gagcgcggcg aagtccgcac tgagctgttg
    29521 aaagaactta accggcagca acgtaaactc cagacctgga ttggcgtgcc tggcgtggac
    29581 cagagccgta ttgaagcatt aattcagcag ttaaaagcgg cggtgagcgt attaatttcc
    29641 gcgccgcgta tcgggcaatt tctgcgtgaa gatcgtttga ttgctctggt gcgtcagcga
    29701 ctgagcatcc caggcggctg ttgcagcttt gatttaccta cattgcacat ttggctgcat
    29761 ctaccccagg cgcagcgcga cagccaggta gaaacctgga ttgccagcct gaacccgctc
    29821 acccaggcac ttaccatggt gctggattta attcgccagt cggccccctt ccgtaaacaa
    29881 accagcctga atggttttta tcaggataac ggtggcgatg ccgacttgct gcgcctgaat
    29941 ctgtcgctcg attcacagct ttatccgcaa atttccggac ataagagccg ttttgccatt
    30001 cgttttatgc cgctggacac tgaaaacgga caggtaccgg aacgtctgga tttcgaactg
    30061 gcctgttgct aaggagtaaa aatgtcagaa actattacgg tgaattgccc aacctgcggg
    30121 aaaacggtgg tgtggggtga aatcagcccg tttcggccat tttgctccaa acgttgtcag
    30181 ctgatcgacc tcggagaatg ggctgctgaa gaaaaacgaa tccccagcag tggcgatctt
    30241 tccgaaagcg atgactggag cgaagagcca aagcagtgac atttgtctga tgcctcacgt
    30301 aggcctgata agacgcggac agcgtcgcat caggcatctt gtgcaaatgt cggatgcggc
    30361 gtgaacgcct tatccgacct acagacgctt aagcttcgca attaccggtt cattggctgg
    30421 cggaaaatca tcggcattaa gaccgaccag cgacatccac tcaccgggtt gcccttcttt
    30481 accccacggc accccttccc aactttcgac cagccagaac cacagagtta tatgcctgtc
    30541 cgggaattca tattccagtt tttcaaatag cgaaaaatgt tgtggggtaa tcccgacctc
    30601 ttcctgaagt tcacgcacca ccgcctgttc cggcgtttcg cccatttcaa ttttaccgcc
    30661 gggaaactcc agtttattcg ccatgtgcgc atctgctgcg cgacgtgtta taaagatttc
    30721 attgttctcg ttgcgaataa tacctaccgc aatttgcagc tttttcattg tcttaaacct
    30781 ataaaaaagg cgcagaatcc tgcgcctttt acttcaactg ttagctttta ttgcaggcga
    30841 ccatggcact gcttgtattt tttaccagaa ccgcacgggc aaggatcgtt acgtcctact
    30901 ttgcgttcac cggtttgcgc cgccagtgca gctgcggctg cagagtcgtc atcctgatgg
    30961 ctaagctgct gcatttgtgc taaacgctcg gcttccatac gacgctgttg ttccagctcc
    31021 tcaacctctt caggcatacg tacctgaact ttgctcagcg tactgataac ttcatatttc
    31081 aacgactcca gcatcgctgc aaacatggag aacgattcac gtttgtattc ctgcttcgga
    31141 tctttctgtg catagccacg caggtggata ccctgacgca gatagtccat cgctgccagg
    31201 tgctctttcc acagggagtc aagcgtttgc agcatgacgc ctttctcgaa gtgacgcatc
    31261 atctcagcac caaccacttc ttctttacgc tgatacactt cgatggactg cgccagaatg
    31321 cgctcacgca gcgtctcttc atgcagttct ggttctttat ccagccactc ggtaattggt
    31381 aaatcgaggt cgaaatcgtt cttcagacgt tcctgcagcc ccggaatatc ccacatttct
    31441 tccagcgact gtggcggaat gtaggcatca atggtggctt tgaacacatc ttcacgaatg
    31501 ctgttgatgg tttcgctcac atcgctgaca tccagcagtt cgttacgctg ggagtaaatg
    31561 gcgcgacgct gatcgttagc cacgtcatca tattccagca gttgcttacg aatgtcgaag
    31621 ttacgacttt caactttacg ctgggcgttg gcaatcgctt tagtcaccca cgggtgttca
    31681 atggcttcgc ctggcttcat acccagttta cgcatcatgc cggatactcg gtcggaagca
    31741 aaaatacgca tcagcgcatc ttccatcgac aggtagaaac gggaagaacc agcatccccc
    31801 tgacgaccag aacgaccgcg caactggtta tcgatacgac gggattcgtg acgctcggta
    31861 ccgatgatat gcaggccacc tgcttccagt accgcatcgt gacgtacctg ccagtcggct
    31921 ttaatttttt caatttgctc tgcggtcgga ttttccagcg cggcaacttc tgcctgccag
    31981 ctacctccga gcacaatatc tgtaccacga cccgccatgt tggtcgcgat agtcaccgca
    32041 gccggataac ctgcctgagc aacaatcgcc gcttcgttgg cgtggaattt ggcgttcagg
    32101 acattgtgct taataccggc tttggtcagt tcgtttgaca ccagctccga tttttcgatg
    32161 gagatagtac ccaccagcac cggctggcct ttcgcagtac gttctttgat atcttcaatg
    32221 atcgcctgaa ttttttccgc ttcagtcatg tagaccaggt ccggcagatc tttacgaatc
    32281 attggacggt tggtcggaac aacgacggta tccagtttgt agatggagct aaattcgaaa
    32341 gcttcggtat cagcagtacc ggtcatcccc gccagttttt catacagacg gaagtagttc
    32401 tggaaggtga tcgaagccag cgtttggttt tcgttctgga tctgcacacc ttctttcgct
    32461 tccacagcct ggtgcagacc atcggaccag cgtcggccct gcatggtacg accggtgtgt
    32521 tcgtcaacga tgataacttc accatcttta acgatgtagt cgacgtcacg ggtaaacagc
    32581 gcatgagcgc gcagcgccgc cgttacgtgg tgcatcagca tgatgttggc cggagagtac
    32641 agagactccc cttcatccat gatgccctct ttcaccagca gttcttcaat cagcaccaga
    32701 ccacgttcgg tcaggttcac ctggcgagat ttttcgtcca ccgagaagtg gccttcgccc
    32761 tggaaggttt cggagtcttc tttttcctga cggatcaggt gcggaataat tttattcacg
    32821 cgtttataca tttccgagct gtcttctgcc gggccggaaa tgatcagcgg tgtacgcgct
    32881 tcatcgatca ggatggagtc cacttcgtcc accagcgcat agtgcagttt acgctgtaca
    32941 cgttcttcag ggctgaacgc catgttgtcg cgcaggtagt caaagccgta ttcgttgttc
    33001 gtaccgtaag tgatgtcagc tgcgtaagct tcgcgctttg ccggtgctgg catgcccggc
    33061 aggttgatac caacagtcag gccaaggaat tcaaacagcg gacggttgtt ttcggcgtca
    33121 cgttgcgcca ggtagtcgtt gacggtaact acgtgcacgc ctttaccggt tagtgcgttc
    33181 aggtaagcag gcagcgttgc ggtcagggtt ttaccttcac cggtacgcat ttcggcgatg
    33241 cagcgttcgt taagaaccat accgccgagt aactgaacgt cgaagtgacg cataccaaag
    33301 acgcgcttac ttgcctcacg caccacggcg aaagcttccg ggatcagatt ttccagcact
    33361 tcgccttttt ccagacgcgc acgaaactct gcggttttcc ctttcagttc ttcatcggag
    33421 agtttttcca tctccggttc catggcattg atgatgttga ccactttgcg catccggcgc
    33481 agggtgcgat cgttacgact accgaaaact ttagttaaca atttgattag cataataaaa
    33541 tctcaaacgc cccgcgttac ggagttaata aaatgaagta aaggtttatt gttgttaggt
    33601 gaggcgttga gggccagcac ggatgccttg cgcctggctt atccagacgg gcgtgctgaa
    33661 ttttgcttgt ggggtaaaat gcgcataatc aatgcgataa cccttttcag acggcgtgcc
    33721 ttcctgggtc agcagcgcgc tgagcgtatc cagtaatgca agatgttgcg cctgaagagg
    33781 caaagattct tcagcaacgg gcagtgtttg cggtgccatt gcgaaagaaa gatgacggat
    33841 taccgtacga atagcatgtt gatgccagta atcaacggaa tagttcgaat tcgggcggcg
    33901 cgtgttcgct tccagcaagg ccaattgacc aaagttaact ttggctgaag gctcgtggtt
    33961 gcgggttgtc gcttttgcgg gcgcgtttgg ttcggcggcg ttgctgagcg caggcaaacc
    34021 taaactcgcc gcgaccatcc ctaataagag atgcggccag aagtagcgtt taccaaactg
    34081 tcgccagcgc gtcagtattc cactcacgtt attgccatcc cgatcgaatt caaacgtatt
    34141 tagcgcacaa atcttatcat taaagcctga agtccacagc acgaatgaag ataagttgtg
    34201 cgtaaaagtg cttaagaaaa cagcgttcgc accgtttttt atagctctta taaaattcaa
    34261 aaaaaaacga ctggttcacc tggccggaga gagtgccaga tttgccagtc gaattttata
    34321 cgacagtata aatgtcgtta tgccagtaca gctgaaggcg ctttgaaggc caacggcagt
    34381 tctgcgtcgt cctggaaggt cacatattcc caggcttcct gtttcgccag gacagcctgc
    34441 agcagtttgt tattcagtgc atgaccggat ttataagcgg taaatgcacc aataatattg
    34501 tgaccacaca tgaacaagtc accgatcgca tcgagcattt tgtgacgcac aaattcgtct
    34561 tcaaaacgca ggccgtcttc gttcagtacg cgataatcgt caacaacgat ggcacaatcg
    34621 aagctgccgc ccaggcacaa accacgggac tgcagatatt cgatatcacg catgaaaccg
    34681 aacgtacgcg cacggctgat ctggcgcata aacgcatcag cggagaagtt catcgcatag
    34741 cgctggttgc tggaatcaat agccggatgg ttaaaatcga tggtgaaatc cagcgaaaaa
    34801 ccattgtacg gcttaaattc agcccactta tcgccatctt cgacacgaac agtctctttg
    34861 atgcgaacaa attttttggc gcagttcaac tcgtcgatac cggcgtcaag cagcaggtat
    34921 acaaacggag cggcgctgcc gtccatgatc gggatttccg gcgcgttaac ttcgataaca
    34981 atgttatcga tgcccaggcc cgcgagagca gcattgaggt gctctacggt tgaaatccgt
    35041 acatcatgct cgttgaccag acacgtacag agcatggtat cacgcacaga tttggcatcg
    35101 gccgggaaat ctaccggtgg attcaagtcg gtgcgacgat agatgacccc ggtgttggcc
    35161 ggcgcagggc gtaacgtcag ggtgactttc ttgccggtat gtaaaccgac acccgtcgcc
    35221 tgaacgatac gtttaagtgt cctttgtttg atcatcgtat tatctcgcca aattacctat
    35281 ccaaccgaag tgtactatac attcggcggg ccagtttagc acaaagagcc tcgaaaccca
    35341 aattccagtc aattcttaat cagcttgctt acgtaggaat gctgggatat ccagataatc
    35401 cggctctttc gcagtttgcg gcgcattgtc attcactact ttagcaaccg gcttctgctc
    35461 ctgggtcagc ggagccatcc catgctgctg gtagcgatcc atcactggct gctgaacctg
    35521 cttattggtc accagagtga tttcaggacg tttgtccatg ccgatacctg tcgcaacaac
    35581 ggttacgcgc agctcgtcat tcatatccgg gtcaagagaa gtaccgataa ccacagtcgc
    35641 gttgtcggaa gcaaatgcac ggatggtgtt acctaccgtt tcgaactcat ccagacgcag
    35701 gtcgaagccc gccgtgatgt taaccagcac gccgcgcgcg ccagacaggt cgatatcttc
    35761 cagcagcgga gaagagatag ccatttcagc agcttcttcc gcacggtctt caccgctcgc
    35821 cacgccagaa cccatcattg cgtagcccat ttcagacatt acggtgcgta cgtctgcaaa
    35881 gtccacgttc atcaaacccg gacgagtaat cagttcggcg ataccttgca cagcgccttt
    35941 cagtacatcg ttcgctgcgc caaacgcatc cagcagggag ataccgcggc ccagaacttt
    36001 cagcagtttg tcgttcggga tagtgatcag agagtccaca tgcttggaca gttcagtgat
    36061 cccctgctcg gcgaatgcca tacgcttctt gccttcaaag ttgaaaggct tagtgacgac
    36121 agcaacggtc aggataccca aatcttttgc cacttcagcg acgactggtg ctgcacctgt
    36181 accggtacca ccacccatac ccgcagcaat aaagaccatg tctgcacctt ccagcgccgc
    36241 acgcaatgca tcgcgatcct catcagccgc attgcggcca acttctggat tagcgccagc
    36301 gcccagtcct ttggtgatac cgctaccgat ttgaatcgtc tgtccaaccg ctgttttacg
    36361 cagcgcttgt gcatcggtat ttaccgcgaa gaattcaaca ccttcaatac gctcgcgcac
    36421 catgtgttca acagcattac cgccgccgcc gccgacgccg atgactttaa tcaccgcgtc
    36481 attggtaagt tccattggtt caaacatagt ttctctccga tttgtgcctg tcgcctgagt
    36541 ccgtaatcat cgtcggcctc ataaaaatta aaactctttt cgcagccaac tattgagtcg
    36601 cttgatccac gagccaactg atgctgtaac acgtttttct acttcagctt caccgttaag
    36661 atgtgactct ttcccatagt gaagcaatcc caccgccgtc gaataatacg gctcctgagc
    36721 ataatccgtt aaaccggtaa tgttcagcgg cgcgccgata cgcacttgcg tatgaaacac
    36781 gcgctgagca caggctgcaa gaccttcgat ctgcgctgcg ccaccggtta atacaatgcc
    36841 tgccgccagg tgatgtttaa ccccttgttg gcgaagcttt tcctgcaact gcaatatctc
    36901 ttcgttgacc aggttgagca actcggtata gcgcggctcg atcacctctg ccagggtctg
    36961 acgttgcaga ctccgcggcg gacgaccgcc tacgctcggc acttccacgc tctcatcttt
    37021 tccaacgatg gaacccagcg cacaaccgtg gcgaacttta atcgcttcgg cgtcgcttgg
    37081 cggcgtgcca aaggcgtaag cgatatcact ggtcacgaca ttgccagcat aaggaattac
    37141 ctttgtgtgg cgcaatgccc caccggtata aacggcgata tccattgtac caccaccgat
    37201 atcgacgacg cagacaccca gttcacgttc atcttccgtc aataccgaat aacttgatgc
    37261 cagtccggca aatatcagtt ggtcaacttt cagcccacaa cgttcaaccg ctttgacgat
    37321 gtttttcgcc atatcgttgt gacatgtgat caggtgcact tttgcctgca tccgcacacc
    37381 cgaaagtcct accggattct tgatcccttc ctgatagtca atcgcatact cttgcgggat
    37441 cacatgcagc acacgatgct catcgcgcac acgcaccgat ttcgcggtat ggacgacgtt
    37501 ttccacatct tcttgcgtca cttcttcttc agaaataggc accataccaa tttcattctg
    37561 gcagctgatg tgcttaccag aaagcgccag atataccgaa gagatctgac aatctgccat
    37621 caattctgcc tggtcaatgg cgcgttgtac gcacttgacc acggattcga ggtcgttcac
    37681 cccgccttta tccataccac gtgacgggca gctgcccacg ccaatgatat tgaccatacc
    37741 gtcgggcaga acttccccta ctaaagcggc aaccttcgcg gtaccaattt ccagtcctac
    37801 taccagtttt ctgtccgtcg ccttgatcat tgttgttctg cctgtgcctg attttgttgc
    37861 tgagtagatt cctctggcgg caagggcgcc cagcctactg ccgctccaga gtcataacgc
    37921 aaatcaacgt agctaatccg tttgccatcg gtttgcgcct gctgctgtaa aaccggataa
    37981 agttctacaa agcgaaccaa acgtttcatc gtatcgcccc ggccaagatt gagcttaata
    38041 tcgttattca gcgtcaactg ccaggaacgc cgcgcggtca tcgccgcttc cttcagagta
    38101 aatctgtcct ttgccagcat ctgccccatt tcgcgatagc cctgcaacac ttcattagcg
    38161 ctgccttccg ggccatacag cattggaagc acctgcttgc tggtgcgatc tggcggcacg
    38221 ctgaaggtgt ttccttccgc gtctaccatg tgttgatcat tccaccgcgc aatcggcaca
    38281 tattcaacca gatgaatctt caattcatca ggccactgct ttctgacgct cacctgctta
    38341 atccacggca ggcgttgttc tatttgcgtc tggatgatgt tgacatcctg ggtcataaag
    38401 gtacccggct cacccaatgc caggatcgac tgccggatat cgtcattacg cgtgtaatgg
    38461 cgttcaccgg tcaacaccag ctttgagagc ggcaggcgtt gcgcatcttc catccagctc
    38521 aacacgaccc agccgctcac caacactgtc gttaaaacgg tcagcaggaa aaggatcccc
    38581 gccagacgcg ttccattatt gcggcgagaa gaaacctctt cttcgctgtt tcgcgtgttc
    38641 agagcagcct gcgacatatt agtccgccag ttccagaatt cgtactacca actgcgagaa
    38701 gctcatacct gcctgacgtg ccgccatcgg caccaggctg tggctggtca tacccggtga
    38761 ggtattggct tccagcagat aaaactgtcc atcactgtcc agcataacgt caatacgtcc
    38821 ccatcctttg caacctaacg tcgtccatgc tttcagcact aatgcctgca aattggcctc
    38881 ttgtgacgct tccagacctg cggggcagaa atactgtgtc tcatcagaga gatacttcgc
    38941 ctcataatca tagaaggttc cggacggttg aatacgtatt gacggtaaaa tttcttcacc
    39001 gagtatcgca accgtgaact ccggcccact tagccatttt tcaatcaata cttcttcatc
    39061 gtgctgaaat gccaatctta atgcatcttg tagagcattt tctgctacta cttttgacat
    39121 tcccacactg gaaccttcgc ggctcggctt aacgataacc ggcaaaccca gagcagaaat
    39181 ttctgctaac tgcttatcgc tcaggccttt ttcaaactct gcgcgggtta acgctaccca
    39241 cggcgcgacc ggtaaaccgg caccttgcca cagaagtttg ctgcgtagtt tatctattga
    39301 aagcgcagat gccatcactc cgctgccggt ataaggcaag cccatcagct cgagcatgcc
    39361 ctgcagcgta ccatcttcac cgccgcgacc gtgtagcgcg ataaacactt tctgaaaacc
    39421 catcgacttc agttgcgtca cgtcgacttc tttcgggtcg acaggatacg catcaatacc
    39481 gccttcacgc agcccggcta acaccgctgc gccagaattc agagaaactt cccgctcagc
    39541 ggaagtcccg cccaacagga ccgcgatttt atcagtcatg ttgttcttcc tccggagttt
    39601 gcggcttcag tttgatttca gctaaagaac gggcaatttt tccgatatta ccagccccct
    39661 gaacgagaat caggtcgtta ccggttaata ccggtgccag catctcggct acctgcgcag
    39721 ggtccggcac cagaatggga tcaattttcc cacgtccacg aattgtgcga cacagcgaac
    39781 ggctgtccgc tcccggaatt ggcgcttcgc cagccggata cacttccagc atcaacaggg
    39841 tatcaacctg cgtcagcaca ttggcgaaat catcatacag gtcgcgcgta cgggtaaaac
    39901 ggtgcggctg aaacagcatt accaggtttt tatccggcca gcctgcgcgc gccgctttaa
    39961 tggtggcgtc aacttccgtc gggtggtggc cgtagtcatc gaccagcatt gccgtaccgc
    40021 ttttaccatt cactggctcc agcgggaatt caccgaggaa atcaaaacgg cgaccagtcc
    40081 cctggaagct ctccagcgca cgcaaaatag cctcgtcgtc aattccctct tccgtagcaa
    40141 ccgcaaccgc agctgcggcg ttcagcgcgt tatgacggcc tggcgcattc agggtgacgc
    40201 acatcggctc tttgtcctga cgcagcagcg taaagtgccc ctgcgggcca atctgctgat
    40261 aatcttctac acgcacgtcg gcatcttcgc tgaagccgta agttgtggtc tgacgtccca
    40321 cacgcggtaa caattcgcgg atcaccggat catcaacaca catcaccgca cgaccgtaaa
    40381 aaggcaggtt gtgcagaaaa ttaataaagg tctgttttaa attctcaaag tcgccctggt
    40441 aggtatccat gtggtcggct tcgatattgg tgacaatcgc caccatcggt tgcagatgca
    40501 ggaacgatgc atcactctca tctgcttcgg caatcaggta ccgaccatgc cccaaacgcg
    40561 catgaacccc cgccgctttt accagcccgc cgttaacgaa ggttgggtcg agccccgctt
    40621 ctgcgtagat gctggaaacc atcgcggtag ttgtcgtttt gccgtgcgtt cctgcaatgg
    40681 cgatgccatg acgaaaacgc attaactcag ccagcatttc ggcacgacgg atcaccggaa
    40741 tacgcgcttc atgagctgcg acaatttccg ggttgtcggc agaaatggcg ctggaaacaa
    40801 cgaccacgct ggcatcacgt acgttttccg ggcgatggtt gaaataaatc gtcgcaccca
    40861 gattcagtaa ctgctgcgtg accggatttg gcgctaaatc ggaaccactg atctgataac
    40921 cttcattggc cagaacttcg gcaataccgc ccataccggc accaccaatg ccgacaaaat
    40981 gtatgtgccg aacgcgacgc atttcgggca cgatggaacg cagttttgcc aattgttgtg
    41041 tattcattct ttacgccatt aacttcttaa attcatacga tgcaaaaggc atcgctacaa
    41101 ttacgcccgg gcagcccggc tcacttcatt tgccactcgc tcggtggcat ccggaatgga
    41161 tgcagcgcgg gcgcgttctg ccatggttaa taaggtttct cgcgaccacc cggccagggt
    41221 gttggcgaca gcatccacgc taagctgtgg ctgctcgata attttggctg cgcccgcttt
    41281 ttccagcggt agcgcattcc agtattgctg gcggtcttta tgttgaaacg gcacaaacaa
    41341 cgccggtagt cctgccgcgg cgatttcact cacagttaaa gcaccggagc ggcaaacgac
    41401 gacatccgcc cacgcatacg ccgccgccat atcatcaata aattcagtca ctttatgctg
    41461 cggttgcccc gcttcggcat acgcctgttc aacggattgt tgcgaacctt tgccgctctg
    41521 atgccagata gtgactgaat cacccagttt cgcagcaacc tgcggcattg tctggttaag
    41581 aatgcgtgcg ccctgggaac cacccactac caacacacga accggacctt cacgtccagc
    41641 caaacgttgc tgcggcaacg gcagcgccag cacatcggta cgcaccgggt tacccaccac
    41701 ttccgcatta gggaaagcgc ctggaaacgc ctgcatcact ttggtagcaa tcttcgccag
    41761 ccatttattg gttaagcccg caataccgtt ttgttcatga agtacaaccg gaatgcctaa
    41821 cgaccacgcg gccagaccac ctggacctga tacatagccg cccataccga gtaccacgtc
    41881 aggtttgtac gctttcataa tcgcccgcgc ctgacgccag gcgttgaaga tacgcagcgg
    41941 cgcagctatc agtgctttta taccttttcc acgcaaacca gagatacgaa tgaaatcaat
    42001 ttcgatgcca tgttttggca ctaagtccgc ttccatacgg tcggcagtcc ccagccagcg
    42061 aacttgccaa ccctgagcca ttagatagtg cgcaaccgcc agtcccggga atacatgtcc
    42121 accggttccg cctgccatca ccattaatcg ctttccttga ccactcatcg tgaacctcgt
    42181 acaaacgcct gcgctttctc cagacgcgtt tcataatcaa tacgcaacag catcatgatg
    42241 gctgtcgaca taatcagtaa gctcgaacca ccgtaactga ttagcggcaa tgtcagacct
    42301 ttggtcggta acatccccgc cgccgcgcct acgttaacca gcgcctggaa gctaaaccag
    42361 atgccaatag aacaggcgag aaaaccggaa aaacggtggt caatttctaa tgctttgcgg
    42421 ccaatcgaca tcgcacgaaa agcgacgaag aataccatta aaagtgctag taccacaccg
    42481 acataaccta attcttcgcc gataatggcg aaaataaagt cagtgtgcgc ttccggcaga
    42541 tactccagtt tttgtaccga attacctaaa ccttgccccc aaagttcgcc gcgaccaaac
    42601 gccatcagcg attgcgttaa ctgatagccg ctgccaaagg gatcttccca cgggttccag
    42661 aatgcggtaa cacggcggat acggtacggt tcggcgagga tcagcaacac aaccgctgaa
    42721 atgcccatac cgataatggc aatgaactgc cacaatttcg ctcccgccag gaacaacatc
    42781 gccagcgtag tcacaaacaa caccaccacc gtaccaaggt ctggctgtgc cagcagtaac
    42841 actgccaaca ccagaatcac gcccatcggt ttcaggaagc cgcgcaggtt attacgtact
    42901 tcgtcgcctt tacgcaccag atagttggcg atatagcaaa acagcgacag ttttgtcagc
    42961 tccgcaggct ggatacgcag caaaccgaga tcgatccaac gcgatgcccc tttaacagcg
    43021 ctacccacta ccagcacgat catcagcagg atgatagatc cgagcagcat cgtggcactg
    43081 tagcgttgcc agaactccat cggcagacgc agcgtaatga tcgccagaat aaacgccaaa
    43141 atcagataga caccatcacg cttcgcgaag aagaacggat cgttggttaa gcgttgccct
    43201 atgggcattg acgccgaggt caccatgata aagccaatcg ccgcgaggcc gaaggtcagc
    43261 cacagtaagg tgcgatcgta catgatcagg ctgtcggtat ctttttcccg cgagcccatc
    43321 acccagccct ttagcgccgt ggagatccag accaggatac tgaatcctgg caggcgcggc
    43381 attttcaggc gagggagaga taaacgcatc aacctaactc cttcgccaga cgggcaaact
    43441 cattgcctcg ttgttcaaag ttcttgaact gatcaaggct ggcacaggcc ggggagagca
    43501 gaaccatatc gcccggctga acacgcggag ccagcaagcg catagcctgt tccatagttt
    43561 cggtttgttc tgccacttcc gggcgtagcg ccgccagctg cgcgccgtca cgaccgaaac
    43621 aatacagacg tacgttatcg ccattcaggt aacgcaccag tgggctaaag tccgccgatt
    43681 taccatcgcc gcccagcaac aaatgcagcg tgccgtctac gtgcaggcca ttcagcgccg
    43741 cttccgtact gccgacgttg gtcgcttttg aatcgttaat ccagcgcaca ccgttatgct
    43801 ccagcaccac ttcaaagcga tgcggcagac cggtgaaggt ggttaacgct ttcaggctgc
    43861 tggcgcgcgg taaccctgcg gcatctgcca gcgccagcgc caccagcgca ttggtgtagt
    43921 tatgctgccc ggaaagtttc atctctttca cattcagcac tttctcgcct ttaacccgca
    43981 gccaggtttc gccctgctga tgattcaggt gatagtcacc catgttgacg ccaaagctga
    44041 cgcagcgttc atccgcaccg cgaatcggca ttgttaaggc atcatcagca ttaaccacgc
    44101 aaactttcgc gttttcgtaa atgcgcagtt ttgctgcacg atactgttgt aaaccaaacg
    44161 ggtagcgatc catatgatct tcagtcacgt tcagaatggt cgctgctacc gcctgtaagc
    44221 tggaggtggt ttccagctgg aagctcgaca gttccagcac gtacagttca cactcatcat
    44281 ccagtagcat caacgcaggc aggccaatat tgccacccac accaacgtta acccccgccg
    44341 ctttcgccat ttcacccact agcgtggtga ccgtgctttt gccgttagaa ccggtaatcg
    44401 ccacaatcgg tgcttgtgct tcgcgacaaa acagctcgat atcgccaacg atttcgattc
    44461 cggcatcagc ggcagcgctt aaggatggat gcgccagtgc aataccggga ctggcgacaa
    44521 tcagatctgc cgccatcagc cattcatcat tcagactgcc cgtgtggcgt tctacggctt
    44581 cgggtaattt atccaggcca ggcggtgtca tacgcgtatc cataacgcgc ggcgtcacac
    44641 cgcgagcgag gaaaaagtcc acgcaggaaa gcccggtgag gcccaggccg ataatgacaa
    44701 catttttacc ctggtaatca gccatgatta acgtaccttc agcgttgcca gaccaatcag
    44761 gaccagcatc agcgaaataa tccagaaacg cacaatgacg cgcggttccg gccagccttt
    44821 cagttcatag tggtgatgaa tcggtgccat gcggaaaata cgttgtccgc gcagtttaaa
    44881 ggagccgacc tgcaggatga cagaaagcgt ttctaccacg aacacgcccc ccataatcac
    44941 cagcaggaat tcctgacgta gcagtacggc gataatgcct aacgcaccac ctaacgccag
    45001 cgaaccgaca tcgcccataa agacctgcgc cggataggtg ttaaaccaca ggaagcccag
    45061 tcctgccccg actatcgcgg tacagacaat aaccagttcc ccggcgtgtc gcagatacgg
    45121 tatgtgtaaa tagctggcaa agttcatgtt gccggtcgcc cacgccacca gcgcaaaacc
    45181 accggcgaca aatacggtcg gcataattgc cagaccgtca agaccatcgg tcaggtttac
    45241 cgcgttgcca gtacccacaa tgacgaagta agccagcaga atgtagaaca gccccagttg
    45301 cggcatcaca tctttaaaga atgggaccac cagctgcgtt gcgggcgtgt ctttgccggc
    45361 aaggtacaaa gcgaaagcaa cacccagcgc aatgaccgac atccagaaat atttccaacg
    45421 agcgatcaac cctttggtgt ctttacgcac cactttgcga taatcatcaa caaagccaat
    45481 aacaccgtaa cctaccagca ccaccaacac gcaccagacg tacggattgg acgggtaagc
    45541 ccacagcagc acggagatca caatcgctgt caggatcata atcccgccca tggtcggcgt
    45601 accgcgcttg ctgaagtgtg attcaggacc gtcgttacgc actacctgac caaaggaaag
    45661 tttttgcaaa tgagcaatca tgcgcgggcc catccacaat gagatgaaca gcgcggtcag
    45721 caggctgacg atggcgcgaa acgtcagata ggaaaagacg ttaaagccgg aataatattt
    45781 gaccaaatgt tcggccagcc aaactaacat gtcccattct cctgtaaagc gcgtactacc
    45841 tcttccatgg cagcactacg tgaaccctta actaaaatcg taattacctg ttgctcagca
    45901 atcagtgatt taagacgcgt aattaacgca gttttatcag caaaatgttc gccaacgccg
    45961 ctggcggtgc tgatagcatg gctttgttta cccatgctta acacgcggtc aataccagca
    46021 gcttttgccg cctcacccac ctgtacatgg caggcttcgc tttcagcgcc cagttccgcc
    46081 atatcgccca ccaccagcac gcggtagccc ggcatttcag ccagtacctg aacagctgcg
    46141 gtcattgaac cgacattggc gttgtaggag tcgtcgagca gcaactggtt ttctgccagt
    46201 tggatgggga acagacggcc aggaacagct ttcagatttg ccagccccgc tttgatagca
    46261 tcaagcgttg cgcccacgga catggagagc gcagcggctg ccagcgcatt cgcaatattg
    46321 tgacgccccg gcaacggcag cagaacatcg acgctaccgg ttggggtttg tagggtaaat
    46381 tccataccgt gcgaggtcac atggatattg gtggcggtga aatcgctgtt ggcggcattg
    46441 ggtgagaaac gccacacttt gcgtgagcca attacgctct gccagttcag ccagtcgttg
    46501 ttgtcggcgt tcataatggc gataccgttt tccggcaggc cgctaaagat ttcacctttc
    46561 gctttcgcga cacccgcaag cgagccaaaa ccttccagat gcgccgctgc caggttgttg
    46621 accagcgccg cttccgggcg agtcagactc acagtccagg caatctcgcc ctgatggttc
    46681 gcgccaagtt caataactgc gtaatcgtat tccggcgtta agcgcaacag cgtcatcggt
    46741 acaccgatgt cgttgttgag attgcctgcc gtataaagcg tgttgccgca ctggcttaaa
    46801 atcgccgccg tcatctcttt aacggaggtt ttgccggagg accccgtcag agcaaccacg
    46861 agcgccggaa cttgctggcg aacccatgca gccagttcac caaacgccag acgcgtatcc
    46921 ttgacgatta actgcggcag gtcgatgtcc agcggacggc taaccagtag tgcgcctgcg
    46981 ccgccagctt tcgcctggtc ggcaaaatcg tgggcatcaa aacgttcacc tttcagggca
    47041 acaaacaggc agcccggcgt cagttttcgg gtatcagtgg ttacagcatc aagggtgata
    47101 tctgcacctt gcagttcacc gttgagaata tcggtaagtt ggctaagggt tacgctaatc
    47161 atgcaatccc ccccagcaga cgcgccaccg tgacgcgatc ggagtagtcc agacgctgat
    47221 tgccaacaat ctggtaatcc tcatggcctt tgcccgcgac cagtaccaca tcattctctt
    47281 tagcctgcat aacggcgcaa gtcaccgctt cagcacggcc ttccatcact ttggcatgtc
    47341 cggcatctaa cattcccgcc agaatatcgt tgatgatggc acgcggttct tcggtacgcg
    47401 ggttatcgtc cgtcaccacc gccacgtcag caaactcttc ggcaattgcg cccatcagtg
    47461 gacgcttacc tttatcgcga tcgccaccac agccaaagac gcaccacagc ttgcccgcac
    47521 agtgcaggcg cgccgcctgt aaggcttttt ccagtgcatc cggcgtatgc gcgtaatcca
    47581 ccaccaccgt cggtttgcct ggcgcagtga acacttccat acgtccgcaa accggttgca
    47641 gacgcgcggc ggttttcagc agatcagcca gtggatagcc cagcgccagc agtgttgcca
    47701 gcgcgagcaa caggttgctg acgttaaaag cgcccatcag atggctttca atttcgccat
    47761 cgccccagct tgaactaaag cgaatggtcg cgccgctgtc gtgatagttc acttcggtcg
    47821 ctttcaacca gcgtccgtga cagttcggat tgatatgatc ttccattgat accgcaaccg
    47881 cgtccggcag ttttgccagc cagcggcggc ccacttcatc gtcggcgtta ataatcgcct
    47941 gaccgcaatg atgttcagaa taaagcagcc atttcgcagc ttcgtagtgt tccatatcac
    48001 catgataatc aaggtgatcg cggcttaagt tggtaaagac cgacgccgga aatttcaatg
    48061 ccgccacacg gtgctgtacc agcccgtggg aggaaacttc cattgcgcaa aaagtcgcgc
    48121 cctgatccac cagccccgcc agctcatgct gaacatcaac tgccgaaccg gtagtatttt
    48181 ctgtcgggat cactttcccc agcagaccgt taccaacggt gcccattacc gcgctggttt
    48241 cgccaagcag ttggctccac tgcgccagaa gctgggtcgt cgtggttttg ccgttggtgc
    48301 ccgttacgcc tacgagacgt aaattgtcag agggttcatg gtaaaagcgg cccgccagtg
    48361 cagataaacg ctcgttgagc tggctgagat agatgaccgg tacgccgtgc atttcacgga
    48421 tttcaccgtc ggtcgcctca tctttcgcct ctgcaataat ggcagccaca ccttgcgcta
    48481 tcgcctgcgg gatatatcga cgcccgtccg cctgatgacc tactacagct acaaagagat
    48541 cgcccgccgc agccacacgg ctgtcgagtg tcatctctcg cagtgctcgc gaaggtgcgt
    48601 ctggcaccca cggagcaaga aggtcgcgca aattacgatc tgccacctgt cccctcgcct
    48661 tgattaatca caaattcatt tttatcgccc gttgtcagcg catccggctc gatgttcatg
    48721 gtacgcaata cgccgcccat gatggcacca aagaccggcg cggaaacggc gccgccgtag
    48781 tatttacccg cctgcggatc gttgataaca acaaccagcg cgaagcgcgg ctgactcgca
    48841 ggcgcaacgc ctgcggtata agcaatatat ttattgatgt agcgaccgtc cggcccgacc
    48901 tttttcgcgg taccggtttt aatggcgata cgatagcctt taatcgccgc cttcacgccg
    48961 ccgccgcctg gtagcgccac gctttccatc atatgcacca cggtgcgaac aatggattcc
    49021 gggaagacac gttcaccggg aaccgggggg tcaactttgg taatcgacag tgggcgataa
    49081 atgccgtagc tgccgatagt tgcgtagact cgcgctaact gtaacggtgt taccattagc
    49141 ccgtagccga aagagaaggt ggccctctct atgtcagacc accgttgttt ttgaggatat
    49201 aagccactgc gttctccgac caaccccaaa ttggtcgctt ttcccagtcc aaaacgtgag
    49261 taagtatcta ctaacgctga ggacggcatc gttaacgcca gcttggaaac accgacgtta
    49321 ctcgacttct gtaatacccc ggtcagggtt aattcgctgt agcgtgccac gtctttgatt
    49381 tcgtggccgt taattcgata aggaatggta ttgagtaccg agttttcccg caccacgcca
    49441 cgttgcaacg cggtcatcac caccatcggt ttaaccgttg agcccggttc aaacacgtcg
    49501 gtgatggtac ggttacgcat cgcctctttc ggcgtgccgc tcagattgtt agggttgtat
    49561 gacgggctgt tagccatcgc cagcacttca cctgtgttga catccaccag cacggcgcta
    49621 ccagattcag ccttgttaaa ggccaccgcg ttgttcagtt cgcgataaac cagcgcctgc
    49681 aggcgttcat caatactcag cgccaggttg tgcgctgcct ggctgtcagt agaagaaata
    49741 tcttcaatta cgcgaccata gcggtcttta cgcacaatgc gctcacccgg ctgcccggta
    49801 agccatttat cgaaactctt ctcaacgccc tcaatccctt gactatcgac gttagtaaag
    49861 ccgatgaggt gagcagtcac ttcgccggac ggatagtaac ggcgagactc ttcacgcaga
    49921 tgaatccccg gcagtttcag ttttttgatg tagtccgcca tgtcagagtt tacctgacgc
    49981 gccagataaa taaagcgccc tttcgggttg gcgttaatgc gggcagaaag ctgatccagc
    50041 ggaatattga gcgcgttagc cagcgccttc cagcggtcac cgacgctgat accgccagcg
    50101 tcatgcactt ctttcgggtc agcccaaatc gcttttaccg gcacgctcac cgctaacggg
    50161 cgaccagaac ggtcagtaat catgccgcgg gaggtggaaa cttgctgaac gcgaagagaa
    50221 cgcatgtcgc cctctttcac cagcatatcc ggggagataa cttgtaacca cgctacgcgt
    50281 ccgagcagaa aagccagcgc caggagaata cagccgcata acaacgcaaa acgccaactg
    50341 ataaagttgg catgttcttc ctgacgtttt ggtttctgcg ttttcgccgc tgctttcatg
    50401 cgtcgcgttt atccttattt ttgcactacg atattttctt gtgacggatc aacatgctgc
    50461 atttgcagct tttccgtggc gatcctttcc acccggctat ggtcgccgag cgcattctct
    50521 tcaaggatca ggttgcgcca ttcaatgtct aaagcatctc gctccagcac cagttgttcg
    50581 cgctgagcgg tcagtaaacg ggtatggtgc gccgtggtta ccacggtcac cgccgtcaaa
    50641 ataatgcaaa tgaacaggca gagtggcagc ttcccaaatc gcaaaagatc gtcaccgata
    50701 acaccaggca atgcatggcg ctcgtggctt cccatcgatc ctttaacttt gcttagagct
    50761 tctgtcactc tgctgatcat gcgttcgtcc tctctgcaat acgcagaact gaactacggg
    50821 cacgagggtt ctcagccacc tcttcttcgc ccggcattaa cttgcctagt gctcgcagct
    50881 gacggccacc cagttttttg agctgctctt cagtcatcgg taaccctgcc ggaacttgcg
    50941 gaccgcggct gttttcacgc ataaaacgtt tcacaatacg gtcttccagc gagtggaagc
    51001 tgatgatcga aagccgccca cccggggcca gcacgttgag cgagcttttt agcgcctgct
    51061 ctatctcctc cagttcactg tttacccaaa tgcgcaccgc ctggaaggta cgggtcgcgg
    51121 gatgtttaaa cttatctttc accggcgttg cagcagccac gacttccgcc agttctttgg
    51181 tgcgggtcat cggctgttcg cggttacgct cgacaatggc gcgggcaatg cgtttggcaa
    51241 aatgctcttc accataggtt ttcaataccc aggcgatatc ggcttcttct gcggtttgta
    51301 gccattcagc ggctgactgc ccacgggttg ggtccatacg catgtccagc ggaccatcgc
    51361 gcataaagga aaagccgcgt tcagcatcat caagttgcgg tgaagagacg ccaagatcga
    51421 ggagaatgcc gtcgatcttg ccaataagat cgcgctcggc aacgtattcg cccagcgcgg
    51481 agaaaggtcc gtggatgatg gagaagcgcg gatcatcaat agtcttcgca acggcgatag
    51541 cctgcgggtc gcgatcgatc gccagcaaac gcccctcttc gccaagctgc gagaggatca
    51601 gacgtgagtg accaccgcga ccaaaagtcc catcaatgta gatgccatca ggacggatat
    51661 tgaggccatt aacggcttca tccagcagca ccgtagtatg tttatagttt tccatcattt
    51721 tatagagaca agtcctgcag tcgctccgat aagtctccgg tagccaactg ctctgcgtcg
    51781 atatcttcct tgacctgttg atgccaggtt gtttcatccc acagctcaaa cttgttgaac
    51841 tgtccaacca gcatcacttc ttttgtcagc ccggcatgtt gccgcagtac tggcgcgatt
    51901 aacaatcgac ctgcgccatc catctgacat tcgctggcat gacctaacag taggcgctgc
    51961 acacggcgct caaccgggtt catgctcaac agacgcgata atttttgctc gataatttcc
    52021 cattcaggca gggggtaaag cagcaggcac gggtgatgaa tgtcaatggt gcaaaccatt
    52081 tgaccggcag cgttctcaag cagctgttcc cgataacggg taggcactga taagcgccct
    52141 ttgctgtcga gattgactaa cgttgctccc cggaacatgc cagcctcacc ccttattccc
    52201 actttgcccc acaatttccc actgaaagga gtttacggag cggaggaaaa gcttgtcaag
    52261 ccagtcacaa ggcataccga ggtaaaaaac ccgatgttta cggctaatat cagcgttgtt
    52321 tattttacgt ccaacgtgcg aagcaaatta acgttatgaa tagctgtaag aaaaaatccc
    52381 tcatcgtcat cactgtttta aaatgactca ctattttcca cagcaaatat aaagtgtcag
    52441 tttgcgacgc gagcggcatt ttaggacata tcttccccgg ttaacagtcc ttgttacgtc
    52501 tgtgtggcgc ttgatgccag cgggtttgcg caaaagcaaa gaaaagtgtt tgttaaatcg
    52561 gcaattcatc gccgcatgta acaaaataat gcaaaaacgc gctgtcgata tcacttaatt
    52621 attgtgaatt tcagctggtg agttaattaa cgggcgggtt taaaaccatt tatcaataat
    52681 agtcaggaat agtcttattt actttaagga tattgat
//
LOCUS       DADLTY010000004        51803 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.004, whole genome shotgun sequence.
ACCESSION   DADLTY010000004 DADLTY010000000
VERSION     DADLTY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.004"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..1003
                     /gene="thiP"
                     /locus_tag="JN334_000206"
     CDS             <1..1003
                     /gene="thiP"
                     /locus_tag="JN334_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter,
                     permease protein"
                     /protein_id="HAY7436481.1"
                     /translation="FMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDPARAAMLA
                     LLQMVCCLGLVLLSQRLSKAIAPGTTLLQGWRDPDDRLHSRICDTVLIVLALLLLLPP
                     LLAVIVDGVNRQLPEVLAQPVLWQALWTSLRIALAAGVLCVVLTMMLLWSSRELRARQ
                     KMLAGQALEMSGMLILAMPGIVLATGFFLLLNNTIGLPQSADGIVIFTNALMAIPYAL
                     KVLENPMRDITARYSMLCQSLGIEGWSRLKVVELRALKRPLAQALAFACVLSIGDFGV
                     VALFGNDDFRTLPFYLYQQIGSYRSQDGAVTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            987..1685
                     /gene="thiQ"
                     /locus_tag="JN334_000207"
     CDS             987..1685
                     /gene="thiQ"
                     /locus_tag="JN334_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HAY7436482.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(1799..2563)
                     /locus_tag="JN334_000208"
     CDS             complement(1799..2563)
                     /locus_tag="JN334_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7436483.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(2649..3527)
                     /gene="araC"
                     /locus_tag="JN334_000209"
     CDS             complement(2649..3527)
                     /gene="araC"
                     /locus_tag="JN334_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HAY7436484.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            3866..5566
                     /gene="araB"
                     /locus_tag="JN334_000210"
     CDS             3866..5566
                     /gene="araB"
                     /locus_tag="JN334_000210"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HAY7436485.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            5577..7079
                     /gene="araA"
                     /locus_tag="JN334_000211"
     CDS             5577..7079
                     /gene="araA"
                     /locus_tag="JN334_000211"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAY7436486.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            7279..7974
                     /gene="araD"
                     /locus_tag="JN334_000212"
     CDS             7279..7974
                     /gene="araD"
                     /locus_tag="JN334_000212"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY7436487.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            8049..10400
                     /gene="polB"
                     /locus_tag="JN334_000213"
     CDS             8049..10400
                     /gene="polB"
                     /locus_tag="JN334_000213"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HAY7436488.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            10565..13471
                     /gene="rapA"
                     /locus_tag="JN334_000214"
     CDS             10565..13471
                     /gene="rapA"
                     /locus_tag="JN334_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAY7436489.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            13483..14142
                     /gene="rluA"
                     /locus_tag="JN334_000215"
     CDS             13483..14142
                     /gene="rluA"
                     /locus_tag="JN334_000215"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HAY7436490.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(14259..15074)
                     /gene="djlA"
                     /locus_tag="JN334_000216"
     CDS             complement(14259..15074)
                     /gene="djlA"
                     /locus_tag="JN334_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAY7436491.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            15329..17683
                     /gene="lptD"
                     /locus_tag="JN334_000217"
     CDS             15329..17683
                     /gene="lptD"
                     /locus_tag="JN334_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HAY7436492.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            17736..19022
                     /gene="surA"
                     /locus_tag="JN334_000218"
     CDS             17736..19022
                     /gene="surA"
                     /locus_tag="JN334_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HAY7436493.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            19022..20011
                     /gene="pdxA"
                     /locus_tag="JN334_000219"
     CDS             19022..20011
                     /gene="pdxA"
                     /locus_tag="JN334_000219"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAY7436494.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            20008..20829
                     /gene="rsmA"
                     /locus_tag="JN334_000220"
     CDS             20008..20829
                     /gene="rsmA"
                     /locus_tag="JN334_000220"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAY7436495.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            20832..21209
                     /gene="apaG"
                     /locus_tag="JN334_000221"
     CDS             20832..21209
                     /gene="apaG"
                     /locus_tag="JN334_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HAY7436496.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            21216..22058
                     /gene="apaH"
                     /locus_tag="JN334_000222"
     CDS             21216..22058
                     /gene="apaH"
                     /locus_tag="JN334_000222"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HAY7436497.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(22136..22615)
                     /gene="folA"
                     /locus_tag="JN334_000223"
     CDS             complement(22136..22615)
                     /gene="folA"
                     /locus_tag="JN334_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HAY7436498.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(22806..24668)
                     /gene="kefC"
                     /locus_tag="JN334_000224"
     CDS             complement(22806..24668)
                     /gene="kefC"
                     /locus_tag="JN334_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HAY7436499.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(24661..25191)
                     /gene="kefF"
                     /locus_tag="JN334_000225"
     CDS             complement(24661..25191)
                     /gene="kefF"
                     /locus_tag="JN334_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HAY7436500.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVMTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(25239..26633)
                     /locus_tag="JN334_000226"
     CDS             complement(25239..26633)
                     /locus_tag="JN334_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7436501.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGSGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(26692..26979)
                     /gene="fixX"
                     /locus_tag="JN334_000227"
     CDS             complement(26692..26979)
                     /gene="fixX"
                     /locus_tag="JN334_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HAY7436502.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(26976..28262)
                     /locus_tag="JN334_000228"
     CDS             complement(26976..28262)
                     /locus_tag="JN334_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7436503.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLCDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(28313..29254)
                     /locus_tag="JN334_000229"
                     /pseudo
     CDS             complement(28313..29254)
                     /locus_tag="JN334_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(29269..30039)
                     /locus_tag="JN334_000230"
     CDS             complement(29269..30039)
                     /locus_tag="JN334_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HAY7436504.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            30395..31909
                     /locus_tag="JN334_000231"
     CDS             30395..31909
                     /locus_tag="JN334_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HAY7436505.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            31940..33082
                     /gene="caiA"
                     /locus_tag="JN334_000232"
     CDS             31940..33082
                     /gene="caiA"
                     /locus_tag="JN334_000232"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HAY7436506.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            33211..34428
                     /gene="caiB"
                     /locus_tag="JN334_000233"
     CDS             33211..34428
                     /gene="caiB"
                     /locus_tag="JN334_000233"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HAY7436507.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            34502..36055
                     /gene="caiC"
                     /locus_tag="JN334_000234"
     CDS             34502..36055
                     /gene="caiC"
                     /locus_tag="JN334_000234"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HAY7436508.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            36164..36949
                     /gene="caiD"
                     /locus_tag="JN334_000235"
     CDS             36164..36949
                     /gene="caiD"
                     /locus_tag="JN334_000235"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HAY7436509.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            36955..37545
                     /gene="caiE"
                     /locus_tag="JN334_000236"
     CDS             36955..37545
                     /gene="caiE"
                     /locus_tag="JN334_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HAY7436510.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(37631..38026)
                     /gene="caiF"
                     /locus_tag="JN334_000237"
     CDS             complement(37631..38026)
                     /gene="caiF"
                     /locus_tag="JN334_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HAY7436511.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(38288..41509)
                     /gene="carB"
                     /locus_tag="JN334_000238"
     CDS             complement(38288..41509)
                     /gene="carB"
                     /locus_tag="JN334_000238"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAY7436512.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(41527..42675)
                     /gene="carA"
                     /locus_tag="JN334_000239"
     CDS             complement(41527..42675)
                     /gene="carA"
                     /locus_tag="JN334_000239"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAY7436513.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(43131..43952)
                     /gene="dapB"
                     /locus_tag="JN334_000240"
     CDS             complement(43131..43952)
                     /gene="dapB"
                     /locus_tag="JN334_000240"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAY7436514.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(44119..45033)
                     /gene="rihC"
                     /locus_tag="JN334_000241"
     CDS             complement(44119..45033)
                     /gene="rihC"
                     /locus_tag="JN334_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HAY7436515.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(45099..46049)
                     /gene="ispH"
                     /locus_tag="JN334_000242"
                     /gene_synonym="lytB"
     CDS             complement(45099..46049)
                     /gene="ispH"
                     /locus_tag="JN334_000242"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAY7436516.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(46051..46500)
                     /gene="fkpB"
                     /locus_tag="JN334_000243"
     CDS             complement(46051..46500)
                     /gene="fkpB"
                     /locus_tag="JN334_000243"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7436517.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(46587..47081)
                     /gene="lspA"
                     /locus_tag="JN334_000244"
     CDS             complement(46587..47081)
                     /gene="lspA"
                     /locus_tag="JN334_000244"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAY7436518.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(47081..49897)
                     /gene="ileS"
                     /locus_tag="JN334_000245"
     CDS             complement(47081..49897)
                     /gene="ileS"
                     /locus_tag="JN334_000245"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAY7436519.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(49940..50881)
                     /gene="ribF"
                     /locus_tag="JN334_000246"
     CDS             complement(49940..50881)
                     /gene="ribF"
                     /locus_tag="JN334_000246"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAY7436520.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(50889..51107)
                     /locus_tag="JN334_000247"
     CDS             complement(50889..51107)
                     /locus_tag="JN334_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HAY7436521.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            51210..51473
                     /gene="rpsT"
                     /locus_tag="JN334_000248"
     CDS             51210..51473
                     /gene="rpsT"
                     /locus_tag="JN334_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAY7436522.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        12363 a        14183 c        13489 g        11768 t
ORIGIN      
        1 ctttatgctc tgtttcgcca gcttcgccac cgtgctatcg ctgggcggcg gtccgcaggc
       61 aaccactatc gagctggcta tttatcaggc gctgagttac gactacgatc ctgcccgcgc
      121 ggcgatgctg gcgctgctcc agatggtgtg ttgccttggg ctggtgctgt tgagtcagcg
      181 actaagtaag gccattgcgc ccggcaccac gctgctgcaa ggctggcgcg atccggacga
      241 tcgtctgcat agccgcattt gcgacacggt gttaatagtg ctggcgctgc tgctgttgct
      301 gccaccgttg ctggcggtga tcgtcgatgg ggtaaatcgc cagttgccgg aagtgctggc
      361 acaaccggtg ctgtggcagg cgctgtggac ctcgttgcgt attgcgctgg cggcaggtgt
      421 attgtgcgta gtgctgacca tgatgctgct atggagcagt cgcgaactgc gggcgcggca
      481 gaaaatgctg gcgggtcagg cgctggagat gagcggcatg ttgatcctcg ccatgccggg
      541 gattgtgctg gctaccggct tctttttact gctcaacaac actatcggcc tgccacaatc
      601 tgctgacggc attgtgattt tcaccaatgc gttaatggcg atcccttatg cgctgaaagt
      661 actggaaaac ccgatgcgcg atatcaccgc ccgctacagt atgttgtgtc agtcgctggg
      721 cattgaaggc tggtcgcgct taaaagtggt cgagctgcgc gccctgaaac gtccactggc
      781 gcaggcgctg gcttttgcct gcgtgctgtc gattggtgat tttggcgtag tggcgttgtt
      841 cggtaacgat gatttccgta ccctgccgtt ttatctctac cagcaaattg gctcctatcg
      901 cagccaggac ggcgcggtca ccgcgttaat tctgctacta ctctgttttc tgctgtttac
      961 cgtgattgaa aaactaccgg ggcgaaatgt taaaactgac tgatatcacc tggctttatc
     1021 accatttgcc gatgcgtttt agcttaacgg tagaacgcgg cgagcaggtg gcaatcctcg
     1081 ggccaagcgg cgcgggtaaa agtaccctgc tgaatttgat tgccggtttt ctgacgccag
     1141 ccagcggttc gctgactatc gatggtgttg atcacaccac gacgccgccg tcacgccgtc
     1201 cggtgtcgat gctgtttcag gagaacaacc tgttcagcca tctgacggtc gcgcagaaca
     1261 tcgggctggg gctaaatccg gggttgaaac tgaacgcggc acagcaggag aaaatgcacg
     1321 cgattgcccg tcagatgggg attgataatt taatggcgcg gttaccgggc gagctttccg
     1381 gcggtcagcg acagcgcgtg gcgttagcgc gttgtctggt gcgcgaacag ccgattttat
     1441 tgctcgatga accgttctct gcgctcgatc cggcgttacg tcaggagatg ctgacgctgg
     1501 tgagcacgag ctgccagcag caaaaaatga cgctattgat ggtgtcgcac agtgtggaag
     1561 atgcggcgcg gatcgccaca cgctcggtgg tggtcgccga cgggcgcatc gcctggcagg
     1621 gtaagactga agaattgttg agcggtaagg cgagtgcttc ggcaatattg gggattacgg
     1681 gttagtatgc actgtcagat gcggcgtgaa caccttatcc gacctacgga cagtgcgttt
     1741 tgtaggcctg ataagacgcg gtcagcgtcg catgaggcaa cccgcacaag acaatcccct
     1801 aaaccccaac cactttacgc aaaatatcga tatacaccgg catcaacggg tggcgaatta
     1861 acaccaccag cgccaccacg ccgatggcag aaatcaacgg cgtcagccac aacaaacggc
     1921 cgcgcgacaa ataatgactt aaacgatcgg ttgctttacc gctgcgccat aaccgccagc
     1981 acagccagcc accaacccac aaaaataccg ccgtcgccag cagcaaccat ttaaactcgc
     2041 cgctctgcat tccggctgga atatcgatcg ccgcgcccgc cagaatccca ggcaggaagt
     2101 aaaacggtgg ccacagcagg cagccgataa tattcggcgt aatgaatttt gccaccggca
     2161 gatccagcat tcccgccacc attggcacca gcggacgcgt cgggccaaca aagcgaccga
     2221 ccagaatggt gaacatgctg tgttgatgca acgcatgttc agttttatcg agtaatgctt
     2281 tgtttttctt cagaaatgac cagcgatgca acggcttttt aaaacgccag cccagccaga
     2341 aggagatcca gtcgcccagc agacaaccga caatccctgc cagccaagcg tgccagaaac
     2401 ttaactcgcc gctgccaatc agcgctccca gccccgccat cagcaccgta ccgggtagaa
     2461 tcaaaccgac cagcgccagc gactccagaa aggccaccaa caccaccgcc atcaatgaat
     2521 acacggtaga ttgggtaata aagtgttcca gcaatgcttg cataatgtgc ctgtcaaatg
     2581 gacgaagcag ggattctgca aaccctatgc tactccgtca agccgtcaat tgtctgattc
     2641 gttaccaatt atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg
     2701 aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg
     2761 tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc
     2821 ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg
     2881 cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata
     2941 tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacacgatta
     3001 tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca
     3061 agcagattca tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt
     3121 tgcccaaaca ggtcgctgaa atacggctgg tgcgcttcat ccgggcgaaa gaaacccgta
     3181 ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa
     3241 acccactggt gataccattc gcgagcctcc ggatgacaac cgtagtgatg aatctctcct
     3301 ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt ctcgtccctg atttttcacc
     3361 accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg
     3421 ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca
     3481 ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc
     3541 ccgccattca gagaagaaac caattgtcca tattgcatca gacattgccg tcactgcgtc
     3601 ttttactggc tcttctcgct aacccaaccg gtaaccccgc ttattaaaag cattctgtaa
     3661 caaagcagga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga
     3721 aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc
     3781 ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccatac
     3841 ccgttttttt ggatggagtg aaacgatggc gattgcaatt ggcctcgatt ttggcagtga
     3901 ttctgtgcga gctttggcgg tggactgcgc cagcggtgaa gagatcgcca ccagcgtaga
     3961 gtggtatccc cgttggcaaa aagggcaatt ttgtgatgcc ccgaataacc agttccgtca
     4021 tcatccgcgt gactacattg agtcaatgga agcggcactg aaaaccgtgc ttgcagagct
     4081 tagcgtcgaa cagcgcgcag ctgtggtcgg gattggcgtt gacagtaccg gctcgacgcc
     4141 cgcaccgatt gatgccgacg gtaacgtgct ggcgctgcgc ccggagtttg ccgaaaaccc
     4201 gaacgcgatg ttcgtattgt ggaaagacca cactgcggtt gaagaagcgg aagagattac
     4261 ccgtttgtgc cacgcgccgg gcaacgttga ctactcccgc tatattggcg gtatttattc
     4321 cagcgaatgg ttctgggcaa aaatcctgca tgtgactcgc caggacagcg ccgtggcgca
     4381 atctgccgca tcgtggattg agctgtgcga ctgggtgcca gctctgcttt ccggtaccac
     4441 ccgcccgcag gatattcgtc gcggacgttg cagcgccggg cataaatctc tgtggcacga
     4501 aagctggggc ggcttgccgc cagccagttt ctttgatgag ctggacccga tcctcaatcg
     4561 ccatttgcct tccccgctgt tcactgacac ctggactgcc gatattccgg tgggcacctt
     4621 atgcccggaa tgggcgcagc gtctcggcct gcctgaaagc gtggtgattt ccggcggcgc
     4681 gtttgactgc catatgggcg cagttggcgc aggcgcacag cctaacgcac tggtaaaagt
     4741 tatcggtact tccacctgcg acattctgat tgccgacaaa cagagcgttg gcgagcgggc
     4801 agttaaaggt atttgcggtc aggttgatgg cagcgtggtg cctggattta tcggtctgga
     4861 agcaggccaa tcggcgtttg gtgatatcta cgcctggttt ggtcgcgtac tcggctggcc
     4921 gctggaacag cttgccgccc agcatccgga actgaaagcg caaatcaacg ccagccagaa
     4981 acaactgctt ccggcgctga ccgaagcatg ggccaaaaat ccgtctctgg atcacctgcc
     5041 ggtggtgctc gactggttta acggtcgtcg cacgccaaac gctaaccaac gcctgaaagg
     5101 ggtgattacc gatcttaacc tcgctaccga cactccgctg ctgttcggcg gtttgattgc
     5161 tgccaccgcc tttggcgcac gcgcaatcat ggagtgcttt accgatcagg ggatcgccgt
     5221 caataacgtg atggcgctgg gcggcatcgc gcggaaaaac caggtcatta tgcaggcctg
     5281 ctgcgacgtg ctgaatcgcc cgctgcaaat tgttgcctct gaccagtgct gtgcgctcgg
     5341 tgcggcgatt tttgctgccg tcgccgcgaa agtgcacgca gacatcccat cagcccagca
     5401 aaaaatggcc agtgcggtag agaaaaccct gcaaccgcgc agcgaacagg cacaacgctt
     5461 tgaacagctt tatcgccgct atcagcaatg ggcgatgagc gccgaacaac actatcttcc
     5521 aacttccgcc ccggcacagg ctgcccaggc cgttgcgact ctataaggac acgataatgg
     5581 cgatttttga taattatgaa gtgtggtttg tcattggcag ccagcatctg tatggcccgg
     5641 aaaccctgcg tcaggtcacc caacatgccg agcacgttgt taatgcgctg aatacggaag
     5701 cgaaactgcc ctacaaactg gtgttgaaac cgctgggcac cacgccggat gaaatcaccg
     5761 ctatttgccg cgacgcgaat tacgacgatc gttgcgctgg tctggtggtg tggctgcaca
     5821 ccttctcccc ggccaaaatg tggatcaacg gcctgaccat gctcaacaaa ccgttgctgc
     5881 aattccacac ccagttcaac gcggcgctgc cgtgggacag tatcgatatg gactttatga
     5941 acctgaacca gactgcacat ggcggtcgcg agttcggctt cattggcgcg cgtatgcgtc
     6001 agcaacatgc cgtcgttacc ggtcactggc aggataaaca agcccatgag cgtatcggct
     6061 cctggatgcg tcaggcggtc tctaaacagg atacccgtca tctgaaagtc tgccgatttg
     6121 gcgataacat gcgtgaagtg gcggtcaccg atggcgataa agttgccgca cagatcaagt
     6181 tcggtttctc cgtcaatacc tgggcggttg gctatctggt gcaggtggtg aactccatca
     6241 gcgacggcga tgttaacgcg ctggtcgatg agtacgaaag ctgctacacc atgacgcctg
     6301 ccacacaaat ccacggcgaa aaacgacaga acgtgctgga agcggcgcgt attgagctgg
     6361 ggatgaagcg tttcctggaa caaggtggct tccacgcgtt caccaccacc tttgaagatt
     6421 tgcacggtct gaaacagctt ccaggtctgc ccgtacagcg tctgatgcag cagggttacg
     6481 gctttgcggg cgaaggcgac tggaaaaccg ccgccctgct tcgcatcatg aaggtgatgt
     6541 caaccggtct gcagggcggc acctccttta tggaggacta cacctatcac ttcgagaaag
     6601 gtaatgacct ggtgctcggc tcccatatgc tggaagtctg cccgtcgatt gccgtagaag
     6661 agaaaccgat cctcgacgtt cagcatctcg gtattggtgg taaggacgat cctgcccgac
     6721 tgatcttcaa tacccaaacc ggtccagcga ttgtcgccag cctgattgat ctcggcgatc
     6781 gttaccgtct gctggttaac tgtatcgaca cggtgaaaac accgcactcc ctgccgaaac
     6841 tgccggtggc gaatgcactg tggaaagcgc aaccggatct gccaactgct tccgaagcgt
     6901 ggatcctcgc tggtggcgcg caccataccg tcttcagcca tgcgctgaac ctcaacgata
     6961 tgcgccagtt cgccgagatg cacgacattg aaatcacggt gattgataac gacacccgcc
     7021 tgccagcgtt taaagacgca ctgcgctgga acgaagtgta ttacggattt cgtcgctaag
     7081 tagccacatc aggtatgtaa cgcctgatgc gacgctgacg cgtcttatct ggcctacacg
     7141 ctgcgatttt gtaggccgga taagcaaagc gcatccggca ttcaacgcct gatgcgacgc
     7201 tggcgcgtct tatcaggcct acgcgctgcg attttgtaga ccggataagc gaagcgcatc
     7261 cggcacgaag gagtcaacat gttaaaagat ctcaaacgcc aggtattaga agccaacctg
     7321 gcgctgccaa aacacaacct ggtcacgctc acatggggca acgtcagcgc cgttgatcgc
     7381 gagcgcggcg tctttgtgat caaaccttcc ggcgtcgatt acagcgtcat gaccgctgac
     7441 gatatggtcg tggttagcat cgaaaccggt gaagtggttg aaggtacgaa aaagccctcc
     7501 tccgacacgc caactcaccg gctgctctat caggcattcc cctccattgg cggcattgtg
     7561 catacgcact cgcgccacgc caccatctgg gcgcaggcgg gtcagtcgat tccagcaacc
     7621 ggcaccaccc acgccgacta tttctacggc accattccct gcacccgcaa aatgaccgac
     7681 gcagaaatca acggcgaata tgagtgggaa accggtaacg tcatcgtaga aacctttgaa
     7741 aaacagggta tcgatgcagc gcaaatgccc ggcgttctgg tccattccca cggcccgttt
     7801 gcatggggca aaaatgccga agatgcggtg cataacgcca tcgtgctgga agaggtcgct
     7861 tatatgggga tattctgccg tcagttagcg ccgcagttac cggatatgca gcaaacgctg
     7921 ctggataaac actatctgcg taagcatggc gcgaaggcat attacgggca gtaatgactg
     7981 tataaaacca cagccaatca aacgaaacca ggctatactc aagcctggtt ttttgatgga
     8041 ttttcagcgt ggcgcaggca ggttttatct taacccgaca ctggcgggac accccgcaag
     8101 ggacagaagt ctccttctgg ctggcgacgg acaacgggcc gttgcaggtt acgcttgcac
     8161 cgcaagagtc cgtggcgttt attcacgccg atcaggttcc ccgcgctcag catattttgc
     8221 agggtgaaca aggctttcgt ctgacaccgc tggcgttaaa ggattttcac cgccagccgg
     8281 tgtatggcct ttactgtcgc gcccatcgcc aattgatgaa ttacgaaaag cgtctgcgtg
     8341 aaggtggcgt taccgtctac gaggccgatg tgcgcccgcc agaacgctat ctgatggagc
     8401 ggtttatcac ctcaccggtg tgggtcgagg gtgatatgca caatggcact atcgttaatg
     8461 cccgtctgaa accgcatccc gactatcgtc cgccgctcaa gtgggtttct atagatattg
     8521 aaaccacccg ccacggtgag ctgtactgca tcggcctgga aggctgcggg cagcgcatcg
     8581 tttatatgct ggggccggag aatggcgacg cctcctcgct tgatttcgaa ctggaatacg
     8641 tcgccagccg cccgctgctg ctggaaaaac tcaacgcctg gtttgcaaac cacgatcccg
     8701 atgtgatcat cggctggaac gtggtgcagt tcgatctgcg aatgctgcaa aaacatgccg
     8761 agcgttaccg tcttccgctg cgtcttgggc gcgataatag cgagctggag tggcgcgagc
     8821 acggctttaa aaacggcgtc ttttttgccc aggctaaagg tcggctaatt atcgacggta
     8881 tcgaggcgct gaaatccgcg ttctggaatt tctcttcatt ctcgctggaa actgtcgctc
     8941 aggagctatt aggcgaagga aaatctatcg ataacccgtg ggatcgaatg gacgaaattg
     9001 accgccgttt cgccgaagat aaacctgcgc tggcaactta taacctgaaa gattgcgagc
     9061 tggtgacgca gatcttccac aaaactgaaa tcatgccatt tttactcgaa cgagcaacgg
     9121 tgaacggcct gccggtggat cggcacggcg gttcggtggc agcgtttggt catctctatt
     9181 ttccgcgaat gcatcgcgct ggttatgtcg cgcctaatct cggcgaagtg ccgccgcacg
     9241 ccagccctgg cggctacgtg atggattcac ggccagggct ttatgattca gtgctggtgc
     9301 tggactataa aagcctgtac ccgtcgatca tccgcacctt tctgattgat cccgtcgggc
     9361 tggttgaagg catggcgcaa cctgatccag agcacagtac cgaaggtttt ctcgatgcct
     9421 ggttctcacg ggagaaacat tgcctgccgg agattgtgac taacatctgg cacgggcgcg
     9481 ataaagccaa acgtcagggt aataagccgc tttcgcaggc gctgaagatc attatgaatg
     9541 ccttttatgg cgtgctcggc accaccgcct gccgcttctt cgatccgcgc ctggcatcgt
     9601 cgatcaccat gcgtggtcat cagatcatgc ggcagaccaa agcattgatt gaagcgcagg
     9661 gctacgacat tatctacgga gataccgact cgacttttgt ctggctaaaa ggtgcacatt
     9721 cggaagaaga ggcggcgaaa atcggtcgtg cactggtgca gcacgttaac gcctggtggg
     9781 cggaaacgct gcaaaaacaa cggctgacca gcgcattaga actggagtat gaaactcatt
     9841 tctgccgttt tcttatgcca accattcgcg gagctgatac cggcagtaaa aagcgctacg
     9901 ccgggctgat tcaggagggc gacaagcagc ggatggtgtt taaagggctg gaaaccgtgc
     9961 gcaccgactg gacgccgctg gcccagcagt ttcagcagga gctgtacctg cgtatcttcc
    10021 gcaacgagcc atatcaggag tacgtccgcc aaaccatcga caaactgatg gcgggtgaac
    10081 tggatgcgcg gctggtttac cgtaaacgcc ttcgccgtcc gctgagcgaa tatcagcgta
    10141 atgttccgcc tcatgtacgc gccgctcgcc ttgccgatga agaaaatcaa aagcgcggtc
    10201 gccccttgca atatcagaat cgcggcacca ttaagtacgt atggaccacc aacggcccgg
    10261 agccgctgga ctaccaacgt tcaccactgg attacgaaca ctatctgacc cgccagctac
    10321 aacccgtggc ggagggaata ctccctttta ttgaggataa ttttgctaca cttatgaccg
    10381 ggcaacttgg gctattttga gcaaaaaaaa gagttcgcca gataccattt tgatgcgtga
    10441 cgaatgcttt gccatccagt accatagcgc cctttccatt cctggacctg aataacacca
    10501 ctacctcata agcacggtag cgggtggtta ttgcctgcaa ttaaagatat agagccgaac
    10561 acatatgcct tttacacttg gtcaacgctg gatcagcgat acagaaagcg aattgggact
    10621 tggaaccgtt gtcgcggtgg atgcgcgaac tgtcacttta cttttcccat ctactggtga
    10681 aaaccgtctg tacgcacgca gtgattcccc cgtgacccgc gtgatgttca accctggtga
    10741 taccattacc agccatgacg gctggcagat gcaagtcgaa gaagtaaaag aagaaaatgg
    10801 cttgctgacc tatatcggta ctcgcctgga tactgaagag tccggcgtag ccctgcgtga
    10861 agttttcctt gatagcaaac tggtgttcag caaaccgcag gaccgtctgt ttgccgggca
    10921 gattgaccgt atggaccgct ttgcgctgcg ttatcgcgcg cgtaagtatt ccagcgaaca
    10981 gttccgtatg ccgtacagcg gcctgcgcgg tcagcgtacc agcctgatcc cgcatcagct
    11041 caacatcgct catgatgttg gtcgccgcca cgcgccgcgc gtcctgctgg ctgacgaagt
    11101 gggtttaggg aaaaccattg aagccgggat gatcctgcat cagcaactgc tctctggcgc
    11161 tgctgaacgt gtgctgatta tcgtcccgga aaccttacag catcagtggc tggtagaaat
    11221 gctgcgccgt ttcaacctgc gctttgctct gtttgatgat gagcgttatg ccgaagctca
    11281 gcacgatgct tacaacccgt ttgacaccga acagctggtg atttgctcgc tggattttgc
    11341 ccgtcgtagc aaacagcgct tggaacatct ctgtgaagcc gaatgggacc tgctggtggt
    11401 cgatgaagcg catcacctgg tgtggagcga agatgcgcca agccgtgaat atcaggccat
    11461 tgaacaactg gcagagcacg tgccgggcgt tctgctgctg accgcgaccc cggaacagct
    11521 ggggatggaa agccacttcg cccgtctgcg tctgctggac ccgaaccgtt tccacgattt
    11581 tgcgcagttc gttgaagagc agaaaaatta tcgtccggtt gcggacgccg ttgccatgct
    11641 gctggcaggt aacaaactga gcaatgacga actgaacatg ctcggcgaga tgatcggcga
    11701 gcaggatatc gagccgctgt tgcaggcagc aaacagcgac agcgaagatg cccagagcgc
    11761 ccgtcaggag ctggtttcga tgctgatgga tcgccacggc accagccgcg tgctgttccg
    11821 taacacgcgt aacggtgtga aaggattccc gaaacgcgag ctgcacacca ttaagctgcc
    11881 gctaccgacg cagtatcaga cggctattaa agtctccggc attatgggcg cacgtaaaag
    11941 tgcggaagat cgtgctcgcg atatgctcta cccggagcgt atttatcagg aatttgaagg
    12001 tgataacgcc acctggtgga acttcgatcc gcgcgttgag tggctgatgg gctacctgac
    12061 cagccatcgc tctcagaaag tgctggtgat ctgcgccaaa gctgccactg cgctgcaact
    12121 ggagcaggta ctgcgcgaac gtgaaggtat tcgcgctgcg gtgttccacg aaggtatgtc
    12181 gattatcgaa cgtgaccgcg ctgccgcctg gtttgccgaa gaagacaccg gcgcacaggt
    12241 actgctgtgc tcagaaatcg gttctgaagg acgtaacttt cagttcgcca gtcacatggt
    12301 gatgtttgac ctgccattca acccggatct actggagcag cgtattggtc gtctggatcg
    12361 tatcggccag gcgcacgata ttcagatcca tgtgccttat ctggagaaaa ccgctcagtc
    12421 ggtgctggtg cgctggtatc acgaaggtct ggatgcattt gagcacacct gcccgaccgg
    12481 acgcactatt tacgatagcg tatacaacga tctgattaac tatctggctt caccggatca
    12541 aaccgaaggc tttgacgatc tgatcaaaag ctgccgcgag caacatgaag cgctgaaagc
    12601 acagctggaa cagggtcgtg accgcctgct ggaaatccac tccaacggtg gcgaaaaagc
    12661 ccaggcactg gcagaaagca ttgaagagca ggatgacgat accaacctga tcgccttcgc
    12721 catgaacctg ttcgatatta tcggtatcaa tcaggacgat cgcggcgaca acatgatcgt
    12781 gctgacgccg tccgatcata tgctggtgcc ggacttccct ggcctgtcgg aagatggcat
    12841 caccatcacc tttgatcgtg aagtggcgct ggcgcgtgaa gatgcacagt ttattacctg
    12901 ggagcatccg ctgatccgca acggtctgga tctgatcctt tctggcgata ccggtagcag
    12961 cacgatttca ctgttaaaaa acaaagcgtt gccggtaggt acgctgttgg tggaactgat
    13021 ttatgtggtt gaagcccagg ctccgaagca gttgcagctc aaccgcttcc tgccaccgac
    13081 gccggtacgt atgctgctgg ataaaaacgg caacaacctg gcggcgcagg tagagtttga
    13141 aacctttaac cgccagctta acgcggttaa ccgtcacacc ggcagcaaac tggttaacgc
    13201 cgtgcagcag gatgttcacg ctatccttca actgggtgaa gcgcagatcg agaaatctgc
    13261 ccgtgcattg attgatgcag cgcgtaacga agccgacgaa aaactgtctg ccgagctgtc
    13321 tcgtctggaa gctctgcgtg cagtgaaccc gaacattcgt gacgacgaac tgaccgccat
    13381 tgagagcaac cgtcagcagg taatggaaag cctggatcag gcaggttggc gtctggatgc
    13441 cctgcgtttg atcgttgtaa cgcatcagta acggagccga aaatggggat ggaaaactac
    13501 aatccaccgc aggaaccctg gttggttatc ctgtatcagg atgaccatat tatggtggtc
    13561 aacaagccga gcggtttgtt gtcagtgccg ggtcgtctgg aagagcacaa agacagcgtg
    13621 atgacgcgca ttcagcgtga ttatccgcag gcagaatcgg tgcatcgtct ggatatggct
    13681 accagcggcg tgattgtagt ggcgctgacc aaagccgcgg agcgggagtt aaaacgccag
    13741 ttccgcgagc gcgagccgaa aaagcagtat gtggcccgcg tctgggggca tccatccccc
    13801 gcagaaggtc tggtggatct gccgctgatt tgcgactggc caaaccgccc gaaacagaaa
    13861 gtctgttacg aaacgggtaa acctgcgcag acggaatatg aagtggtgga gtatgcggcg
    13921 gataacacgg caagagtggt gttaaaaccg attaccgggc gttcgcatca attgcgtgtg
    13981 catatgctgg cgctggggca tccgattctc ggcgatcgtt tttatgcatc accagaagcg
    14041 agagcgatgg caccgcgttt gttgctacat gcagagatgt tgacgattac ccatccggcg
    14101 tatggcaata gtatgacgtt taaagcgcca gcggattttt aatcgtcacg ctggcgcagt
    14161 tcggtaggcg gataaggcgt tcacgccgca tccggcaatt gtgcgccgat tgcctgatgc
    14221 gacgcgggcc gcgtcttatc aggcctacag tttaaaactt atttaaaccc tttctgctgc
    14281 tttatcagct catatgcctg ctgaatttcc tgcgctttct gcttcgccat ctccatcatc
    14341 tcaggcggca aacctttcgc caccagctta tcgggatggt gttcactcat cagcttacgg
    14401 taggcacgtt tgatggtggt cgcatcatcc gtcggcttca cgcccagcac attacaggca
    14461 tcttccagcg tcgggccacg ctgcgcttgc tgccagttac caccgccaga ttgctgctga
    14521 taaccgccgc caaactgtgc accgccctgc atcatgcgca aaaactggtc aaactgggcg
    14581 cgggagatcc ctaattcttc agcaatgaca tacagcaccg cccgttcatt cgggtgcagt
    14641 gaaccatcag caaacgccgc ctgaatctgg atctccagaa acatacgaat taagtcaaaa
    14701 cgaccaaagc agacactgcg aaactggcgc atcttttcgc gcagcgggta attatctgat
    14761 tttcccaccc ggaacgcgtt ttgcgccgca gtacgggaag cgccatgaag gttcattcgg
    14821 tccatcaact ggctggcgat atgaatatca gcctccgtga cgcgaccttt ggatttggtt
    14881 aaatgcccca tcacttcaaa agtggtggca aaaaacagcg cctgacgctc acgctggttg
    14941 gcgaaccacg ccattttacg gctacgggct ttatcaaaca tatggccaat taacaggcct
    15001 aacactacgc cccaaaagcc gccgcccatc agtaaggcca cggccacgcc aatgattttt
    15061 ccccaatact gcatatattc cccaaatcga cacacggata tcagggctat ctcccacaat
    15121 ataaaggtgc ttttaccgtt ttccggctgc ggtcaattgt gggacatcgc ctataatttg
    15181 cattatcata cctgtcattc aatgccgtgc ctaacaccac tgacgctatt cggacaggat
    15241 taacactagc gtagagatga cgagtacgtt agtctctgac cgtttgtcac gcgcaacgtt
    15301 accgatgatg gaacaataaa atcaacgtat gaaaaaacgt atccccactc tcctggccac
    15361 catgattgcc accgcccttt atagtcaaca gggactggca gccgacctcg cctcacagtg
    15421 catgttgggc gtgccaagct atgaccgtcc tctggtacag ggcgatacca atgacttacc
    15481 cgtgactatc aatgctgacc acgcgaaagg ggactacccg gatgacgccg tgtttactgg
    15541 cagcgtggat atcatgcagg gtaacagccg tctgcaggcc gacgaagtgc agctccatca
    15601 aaaagaggca ccaggacaac cggagccggt acgtaccgtt gatgcgctcg gtaatgtcca
    15661 ttacgacgat aaccaggtga tcctcaaagg gccgaaaggc tgggcgaatc tgaacaccaa
    15721 agataccaac gtctgggaag gtgattacca gatggtgggt cgccagggtc gcggtaaagc
    15781 ggacctgatg aaacaacgtg gcgaaaaccg ctataccatt ctggataacg gtagctttac
    15841 ctcctgtctg ccgggttctg acacctggag cgtggtaggt agcgaaatta ttcatgaccg
    15901 cgaagaacaa gttgctgaga tctggaacgc ccgctttaag gtgggtccgg taccgatctt
    15961 ttatagcccc tatttgcagt tgccggtggg tgacaaacgt cgctctggtt tcttgatccc
    16021 gaacgccaag tacaccacca ccaactactt tgagttctac ctgccatatt actggaacat
    16081 cgcgccaaat atggatgcca ccatcacgcc gcattatatg catcgtcgtg gcaacatcat
    16141 gtgggagaac gaattccgct acctctccca ggcgggcgct ggcttgatgg aactggacta
    16201 tctgccttca gataaagtct atgaagatga acacccgaac gatgacagtt cacgtcgttg
    16261 gttattctac tggaaccact ccggggtcat ggatcaggtg tggcgtttca acgtcgacta
    16321 caccaaggtc agcgatccta gctacttcaa tgatttcgat aacaagtacg gttccagtac
    16381 tgacggctac gcaacgcaaa aattcagcgt tggctatgcg gtgcaaaact tcaatgccac
    16441 cgtttcaacc aagcagttcc aggttttcag cgaacagaac accagtagct actcggcaga
    16501 gccgcagtta gacgttaatt actaccagaa tgatgttggt ccgtttgata cgcgtattta
    16561 cggccaggca gtgcactttg ttaacaccag agacgacatg cctgaagcaa cccgtgttca
    16621 cctggaaccg accatcaatt tgccgctctc taataactgg ggcagcatca ataccgaagc
    16681 gaagtttctg gcaacccatt atcagcaaac caatcttgac tggtataact ccagaaacac
    16741 gaccaagctg gacgaatccg ttaaccgcgt aatgccgcaa ttcaaagttg acggcaaaat
    16801 ggtctttgaa cgcgatatgg aaatgctggc tccgggttat acccaaacgc tggaaccgcg
    16861 cgcgcagtat ttgtacgtgc cgtatcgcga tcagagcgac atctataact acgactcgtc
    16921 tctgctgcaa tctgactact ctggcctgtt ccgggaccgg acttacggcg gtcttgaccg
    16981 tattgcctcc gctaaccaag tgacgaccgg tgtcacatct cgcatatatg atgatgctgc
    17041 cgttgaacgt tttaatattt ccgttggtca aatctactat ttcacggagt ctcgcactgg
    17101 cgatgacaac ataacatggg agaatgacga caaaacgggc tcactggtgt gggcaggcga
    17161 cacctactgg cgtatctccg agcgttgggg attgcgtggc gggattcagt acgatacacg
    17221 tctggataac gtagcgacca gtaactccag cattgaatac cgtcgggatg aagaccgtct
    17281 ggtacagctg aattaccatt acgccagccc ggaatatatt caggctacgc tgcctaagta
    17341 ctattccact gctgagcaat ataagaatgg tatttcgcag gtaggtgctg tcgccagcag
    17401 gccaattgcc gatcgttggt ccattgttgg ggcctactac tacgacacca atgcgaacaa
    17461 gcaagccgac tctatgttag gtgtgcaata cagctcctgc tgctatgcaa ttcgcgtcgg
    17521 ttacgagcgg aagctgaacg gttgggataa cgataaacaa catgcggtat atgacaacgc
    17581 aatcggcttt aacatcgaac ttcgcggcct gagctccaac tacggtctgg gtacgcaaga
    17641 gatgctgcgt tcgaacattc tgccgtatca aaacactttg tgatttgttg atttaccacg
    17701 taatccgcag tgcggttaat tgaaatggaa aaagtatgaa gaactggaaa acgctgcttc
    17761 tcggtatcgc catgatcgcg aataccagtt tcgctgcccc ccaggtagtc gataaagtcg
    17821 cagccgtcgt caataacggc gtcgtgctgg aaagcgacgt tgatggatta atgcagtcgg
    17881 taaaactgaa cgctgcgcag gcaaggcagc agcttcctga tgacgcgacg ctgcgccacc
    17941 aaatcatgga acgtttgatc atggatcaaa tcatcctgca gatggggcag aaaatgggag
    18001 tgaaaatctc cgatgagcag ctggatcagg cgattgctaa catcgcgaaa cagaacaaca
    18061 tgacgctgga tcagatgcgc agccgtctgg cttacgatgg tctgaactac aacacttatc
    18121 gtaaccagat ccgcaaagag atgattatct ctgaagtgcg taacaacgag gtgcgtcgtc
    18181 gcatcaccat cctgccacag gaagtcgaat ccctggcgca gcaggtgggt aaccaaaacg
    18241 acgccagcac tgagctgaac ctgagccaca tcctgatccc gctgccggaa aacccgacct
    18301 ctgatcaggt gaacgaagcg gaaagccagg cgcgcgccat tgtcgatcag gcgcgtaacg
    18361 gcgctgattt cggtaagctg gcgattgctc attctgccga ccagcaggcg ctgaacggcg
    18421 gccagatggg gtggggccgt attcaggagt tgccggggat cttcgcccag gcattaagca
    18481 ccgcgaagaa aggcgacatt gttggcccga ttcgttccgg cgttggcttc catattctga
    18541 aagttaacga cctgcgcggc gaaagcaaaa atatctcggt gaccgaagtt catgctcgcc
    18601 atattctgct gaaaccgtcg ccgatcatga ctgacgaaca ggcccgcgtg aaactggagc
    18661 aaattgctgc tgatatcaag agtggtaaaa cgacttttgc tgccgcagcg aaagagttct
    18721 cgcaggatcc aggttctgct aaccagggcg gtgatctcgg ctgggctaca ccagatattt
    18781 tcgatccagc cttccgtgac gccctgaccc gcctgaacaa aggtcaaatg agtgcaccgg
    18841 ttcactcttc attcggctgg catttaatcg aactgctgga tacccgtaat gtcgataaaa
    18901 ccgacgctgc gcagaaagat cgtgcatacc gcatgctgat gaaccgtaag ttctcggaag
    18961 aagcagcaag ctggatgcag gaacaacgtg ccagcgccta cgttaaaatc ctgagcaact
    19021 aatggttaaa acccaacgtg ttgtgatcac tcccggcgag cccgccggga ttggcccgga
    19081 cttagttgtc cagcttgcac agcgtgagtg gccggtcgaa ctggttgttt gtgccgatgc
    19141 cactctcctt accgaccggg cagcgatgct cggtttgccg ctcaccctcc gcccttattc
    19201 ccccaactcc cctgcacaac cgcaaactac gggcacatta acgctacttc ctgtcgcgct
    19261 acgtgaatct gtcactgcgg ggcagttagc gattgaaaat gggcattacg tggtggagac
    19321 gctggcgcga gcgtgtgatg gctgtctgaa cggtgaattt gctgcgctga tcacaggccc
    19381 cgtgcataaa ggcgtcatta acgacgcaag cattccgttt accggtcata ccgagttttt
    19441 cgaagagcgt tcgcaggcga aaaaagtggt gatgatgctg gcgaccgaag aacttcgcgt
    19501 ggcgctggca acgacgcatt taccgctgcg cgatatcgca gatgctatca cccctgcact
    19561 tttgcacgaa gtgattgcta ttttgcatca cgatttgcgt acaaaatttg gtattgccga
    19621 accgcgcatt ctggtctgcg ggctgaatcc gcacgcgggc gaaggcggtc atatgggtac
    19681 ggaagagata gacaccatta ttccggtgct cgacgagctg cgggcgcagg ggatgaaact
    19741 caacgggccg ctgcctgccg ataccctgtt tcagccgaaa tatctcgata acgccgacgc
    19801 cgtgctggcg atgtaccacg atcagggtct tcccgtgcta aaataccagg gcttcgggcg
    19861 cggtgtgaac attacgctgg gcctgccctt tattcgcaca tcagtggacc acggcaccgc
    19921 gcttgaactg gcgggacgtg gcgaagccga tgtcggcagt tttattacgg cgcttaatct
    19981 cgccatcaaa atgattgtta acacccaatg aataatcgag tccaccaggg ccacttagcc
    20041 cgtaaacgct tcgggcaaaa ctttctcaac gatcagttcg tgatcgacag cattgtttct
    20101 gccattaacc cgcaaaaggg ccaggcaatg gtcgaaatcg gccccggtct ggcggcattg
    20161 accgagccgg tcggcgaacg tctggaccag ctgacggtca tcgaacttga ccgcgatctg
    20221 gcggcacgtc tgcaaacgca tccattctta ggcccgaaac tgacgattta tcagcaggat
    20281 gcgatgacct ttaactttgg cgaactggcc gagaaaatgg gtcagccgct gcgtgttttc
    20341 ggcaacctgc cttataacat ctccacgccg ttgatgttcc atctgtttag ctatactgat
    20401 gccattgccg acatgcactt tatgttgcaa aaagaggtgg tgaatcgtct ggttgcagga
    20461 ccgaacagca aagcgtatgg tcgattaagc gtcatggcgc aatactattg caacgtgatc
    20521 ccggtactgg aagttccgcc gtcagccttt acaccaccac ccaaagtgga ttccgccgtc
    20581 gtgcgcctgg tccctcatgc aacgatgcct cacccggtta aagatattcg cgtgttgagc
    20641 cgcatcacca ccgaagcctt taaccagcgt cgtaaaacca ttcgtaacag cctcggcaac
    20701 ctgtttagcg tcgaggtgtt aaccggaatg gggatcgacc cggcgatgcg agcggaaaat
    20761 atctctgtcg cgcaatattg ccagatggcg aactatctgg cggagaacgc gcctttgcag
    20821 gagagttaac gatgatcaat tcgccccgag tgtgtattca ggttcaaagc gtctacattg
    20881 aggcacaatc ttcacctgat aatgaacgtt acgtttttgc ttataccgta accatacgca
    20941 atctggggcg agcgccagtg cagttgttgg ggcgttactg gctgatcacc aatggcaatg
    21001 gccgtgaaac cgaagtccag ggcgaaggag tggttggcgt ccagccactt atcgcgcctg
    21061 gcgaagagta ccagtacacc agcggtgcaa tcattgaaac cccgctgggc accatgcagg
    21121 gtcactacga aatgatcgat gaaaatggcg tccctttcag catcgacatt cccgtattcc
    21181 gactcgccgt tcccacactc attcattaaa agaatatggc gacatacctt attggcgacg
    21241 ttcatggttg ttacgatgaa ctgatcgcat tgctgcataa agtagaattt acccccggga
    21301 aagataccct ctggctgacg ggcgatctgg tcgcgcgcgg cccgggttcg ctggatgttc
    21361 tgcgctatgt gaaatcctta ggcgacagcg tacgtctggt gctgggcaat cacgatctgc
    21421 atctgctggc ggtatttgcc gggatcagcc gcaataaacc gaaagatcgc ctgacaccgc
    21481 tgctggaagc gccggatgcc gacgagctgc ttaactggct gcgtcgccag cctctgctgc
    21541 aaatcgacga agagaaaaag ctggtgatgg cccacgcagg gatcacgccg cagtgggatc
    21601 tgcagaccgc caaagagtgc gcgcgcgatg tagaagcggt gctgtcgagt gactcctatc
    21661 ccttctttct tgacgctatg tacggcgata tgccaaataa ctggtcaccg gaattgcggg
    21721 ggctgggaag actgcgtttt atcaccaacg cctttacccg tatgcgtttt tgcttcccga
    21781 acggtcaact ggatatgtac agcaaagaat cgccggaaga agcccctgcc ccactgaaac
    21841 cgtggtttgc gattcctggc cctgtcgctg aagaatacag catcgccttt ggtcactggg
    21901 catcgctgga aggcaaaggt acgccggaag gtatttacgc gctggatacc ggctgctgct
    21961 ggggtgggtc attaacctgc ctgcgctggg aagataaaca gtattttgtc cagccgtcga
    22021 accggcataa ggatttgggc gaagcggcgg cgtcttaaac acagcctgat acaggaaggc
    22081 cggataagac gcgaccggcg tcgcatccgg cactagccgt aaattctata caaacttacc
    22141 gccgctccag aatctcaaag caatagctgt gagagttctg cgcatcggcg tcgtggaatt
    22201 cgctgaatac cgattcccag tcatccggct cgtaatccgg gaaatgggtg tcgccttcca
    22261 cttctgcgtc gatatgcgtc agatacagtt tttgcgcttt tggcaggaac tgctcataaa
    22321 cgcgaccgcc gccaatcacc atgatttctg gtacgtcacc acacgccgca atggcttcat
    22381 ccaccgattt cacccacgtt acgcgatcgt ccgtacccgg ttgactgctg aggataatat
    22441 ttttgcgtcc tggcaacgga cgaccgattg attcccaagt atggcggccc ataatcacgg
    22501 gtttatttaa ggtgttgcgt ttaaaccagg cgagatcggc aggcaggttc cacggcatgg
    22561 cgttttccat gccgataacg cgatctaccg ctaacgccgc aatcagactg atcattgaga
    22621 tttcccgata aaaaaattgt cgccactata cgtaaagcgt aaaccgtcgt cgactggtgc
    22681 gaggatgatg ttgaggaaaa ttttatattc tgctggcgag tccacgctct ccccctggac
    22741 tcgccgcatt acaatgaaac aaaaacaaac agttagctgt aaagtgtgat ttacgtcact
    22801 ctttattaag atgagggttt cgtttccggt tcatccgcca tgttgccggt atgtttacct
    22861 tcttctgttc cctgccagcc atgtcgttga attaatgaca gatgttcgcg gtcctcggtg
    22921 atgatctcac ttaacatcgc gctggtgcgt ttatagaccg ccgcgcgggc tttggtgtca
    22981 ttctcaacca ttgccatctc ttccaccatc tgaatattaa agcggcggaa cacatcggca
    23041 cgttctcgcg cttcatacgg ccccagacct aaactttcca gcgccagacg cccggttttc
    23101 agcgcacctt caaaggtttc acgctccggc ttttcaacgc ctgcctgacg caaacgaatg
    23161 tagtgatcga catcgcgggc gcgggcaata atctgcaaat gcgggaaatg ttctttcacc
    23221 atctccgtca gttgcaggtt ggtttgcgga tcgtcaatag cgttaatcag cacttccgct
    23281 ttcgccgctc cggcagattc cagtaaatcc atccgcgtgg catcgccata aaacactttc
    23341 ataccaaatt tacgcagcgt ttcgatatgg tccggatcgt gatcgagcac caccattttc
    23401 accccgctgg agagcagtaa acgtccggta atctgtccaa aacgaccgaa tccggcgata
    23461 atcacgcgcg gctgttcttc atcgatctca tcggcttcac gcgcctcctc cgtagaagat
    23521 tgctcaaggc gattgaggat caccagcaga atcggcgttg ctgccatcga cagcgccacc
    23581 gccagggtca gcgatttcgc ccactccggc tccagcacat tcgccatctg cgccgcacca
    23641 aataccacaa aggcaaactc actgccctgc cctaacaaca ccgcaaacca gcgacgctgt
    23701 ttatttggca cctgcaacgg tcgggcaatc agccacagca tggcgatttt gatgatgagg
    23761 aaaccgagca gcaaaatgac aatgcgcaat gggttttcaa tcagcgtgcc aaagtctatc
    23821 gacatgccaa caccgatgaa aaacagcccc aacagcaaac ctttaaacgg ttcgatatcg
    23881 ctttccaacg catgacggta ttccgagctt gccagcagta cgcccgccag aaacgcgccc
    23941 atcgccatcg acaagccgac ctcttccagc agcaaaccaa agccaaacac aaggaataac
    24001 gccacggcac taaacacttc ccgcaagcca gagcgggcaa caaaacgcag cgccggacgc
    24061 gtaacatagc gccccagcaa taccaccagt accagcgcac ccgccacttt taacgccgag
    24121 agagcaaatg cgcccatcgt cgtcgaggca ctgctcgccg ccagtagcgg gatcatcgcc
    24181 accagcggga ttgccgcgat atcctggaac agcagcactg caaaggcact gcggcccatt
    24241 tgcgtcacca tcagattgcg ttcattcatc gcctgcatgg caatcgccgt tgatgagagc
    24301 gccagcgtca taccgatcaa ttccgcgacc tgccagcgca gcccaagtaa catacagaac
    24361 agccccagca ggccaccgca aatcaccatc tgcaatgcgc caccgccgaa cactgccgca
    24421 cgcagcttcc acagcctttg tggatcgagt tcgaggccga taataaacag catcagcacc
    24481 accccaatct cggcaaagtg cagaatagat tcggcatcgg tcaccagtcg cagcccccac
    24541 gggccaataa tgcagccggc gatcaggtag ccaagtaccg atcccagacc aagacgtacc
    24601 gcaatgggta caatcagcgc cgccgatccg aggtagatca gcgcctgaac cagcgtatga
    24661 ctatccatga tgggcctcct gccattccag cagacgttgc ttatagtggc gcgcctgccc
    24721 ttcgagggtt tcgtcgtcac aaataaaggt gcagtgcatg gcaaacggtg gcagccagtt
    24781 cagcccgcag tagattgccg tcgcctgtag cggctgcgac agcacatcaa agcccggatg
    24841 cgcaccaatt tcaaaatggc tttccccgcc gccggtcatc accgcccaca gcaaatgttt
    24901 gccatgcagc gccgtgccgc cgtgaccgta agcccagccg tgcgacaaaa ctttatcgat
    24961 ccaaagttta agaagcggag gaatgctgta ccactgcatc gggtgctgcc agacgatcag
    25021 atcggcgcga gagagcgcct cctgctcggc ggcaatatcg atattgaagt caggatagag
    25081 ttgataaaga gagcgaattt cgacgccttc cagcgtcctt gcctgttcaa gcatccgttt
    25141 attcgcatgg gaatgatgcg gatacggatg cgcataaatg atgaggatca tagttattag
    25201 cccgcgtgtt tacgcctttg ttttcccaca aagtgtagtc atatattcac gcggcttata
    25261 gtcaataata ttgattcaga aaagagagaa atctgaacaa ataatcccat tctctgcccg
    25321 cggatcgtca tattgctggt ctgcgccagt gacattcctc gagtctccgg ggcaaacgct
    25381 acggaaatca acaagccaaa cagcgagata cccgccccca ttagcatcgt gttactgata
    25441 ccgtaattat tgataaatat cggcagtgcc caggtcgaaa caatggtgcc aatacgactt
    25501 aaggacataa tcacgcccac ggcagaggcg cggatatccg tcgggaagag ttcattagga
    25561 tagagccact gcaaattacc cggcccgcca gagaaaaagg catacaccgc aaaggccatc
    25621 actaccagcc agatccccat atccgggatt agccccaaaa ccgccaacgc cagcgtcatc
    25681 atggcaaagc tgccaatcaa caatggacgc cgtccggcag tgtttagcca cagcatcggc
    25741 ggaatacagc cgagcataaa gaacaggcta atcaccacat tccctagtgc cgcgtttttg
    25801 ccaaccccca atcccaacga accaacgatt tgcgggccaa aggtgtaaat ggcgaacatt
    25861 gggatcacct ggcaggtcca gatggcggca acaaacagaa caaaaggaaa atggcggcga
    25921 ttaaacagat cgcgaaaacg ggtttgctgc ggctgctctt catcgaaagc caccggttcg
    25981 ccaaacagtt ttatcatcat ctcttcgcac tcttttactc gccctttgcg taataaccag
    26041 cggggagatt ctggcagttc gaatcgacca atcaaaatca acaaacaagg gatcgccgcg
    26101 ctacccagca tccagcgcca accaccttcc acatcataaa gccagtagcc gaccagatcg
    26161 gcgcaggtcg cgccgacata ccacatcgcg gcaataaagc tgatggaaaa cgcccgctga
    26221 cgggtactgg agaactcggt gatcatcgaa gtggcgatgg gataatctgc accgatgaca
    26281 atgccgatga gtacccgcat caccaacagt tcgacggggg atgaaacaca catcgtcgcc
    26341 accgatatca cgccgatggc gatgatatca atgaggaaca ttttgcgccg tccgacttta
    26401 tcggagatat agccaaacag cgatgtgcca acgaacagcc cggcgagcgt tcccgagccc
    26461 agcaagccaa tccagtcagc gtccagtttc agcgccggag tcagttgctc cagcgccacg
    26521 ccaatcatta ccagtacata accatccaga aacggaccac cgcttcccca cagcaaaatg
    26581 cggcggtgaa tagaggagaa tttgagatcg tcaaagtttc tggacggttg catgggttat
    26641 tcctgttttt ttgcaatgag agtcagtacg gcgttacgta tcaaaccaac atcagccgta
    26701 acggaactcc acaccaaagg tgccgcgcgg gtattcccac tgttccagcg ccgaccccag
    26761 ccccagaatg cgacaggtgc cgcactccag acatccggcg taatcgaagc gcacactgcc
    26821 gtcatcctgc ttcttgtaca gacctgcggg gcacgctttc accagcagct ccagcacctg
    26881 tttatcagca tcggccttca caacaatgtg cggatgctct tcatcgacat tgaatttatt
    26941 gacgcccagt ttgacgtcca cattgacggg agaagtcata aaacggtcac tcctttcatg
    27001 ccatccttga tcagattgat gaagcccact ttcttgccgt ggcggaggat tttcttgcgc
    27061 atcagttccg gcgcactgcc gtcaatggtg aacaggtcac gcgccacgcc caccgccagt
    27121 tccgggttgc cgctaaacat gcgtgggtta tcaaggaacg ccggtagttt ctggtacatg
    27181 cgcatatcgc acagcgggcc actctccaga tgctgacgat attccgcgag tttttgctta
    27241 ctgaaatcgt cgcttttcat cgctgaaagc acggtttttg ctgcagcttc cccggcggca
    27301 atcgccagat ccataccgcg aatggtaaaa ccgaggttca tacacattcc ggcggcatca
    27361 ccggcaatca atacgccgtc accgaccagc tccggcagca tgttgatgcc tgcttccggc
    27421 actacgtgag cggaatattc caccagcttg ccgcccgcga tcagcggtgc aacggccgga
    27481 tgctgtttga aatcttccag catttgcggc accgattttt tcgcgtcatg cagatgatgc
    27541 agaccacaaa ccagccccag cgacagggta ttttcattgg tataaaggaa gccgccgccc
    27601 atcaggccat cggtgggtga tcccgcaaac agacaagccg cgccctgatt accctgcaac
    27661 tgaaaacggt cttcgattac cgacttcggt aactcgatca gttccttcac gccaaccgcc
    27721 acatccgtcg gtttaacgcg ttttgccatc cccaactttt cggcaaggat ggagttcacc
    27781 ccatcagcaa ggagcaccgt tttcgcttca atcacatcac catcggcttc tacaccgacg
    27841 actttgccat cgcgctgtac gaggttatcg acgcggatcc cggtaattaa ctgcgcgccc
    27901 gcttcttcgg cctgctccat caaccaggca tcaaatttac tgcgcaaaac ggagtaagaa
    27961 cgctgggatg gcgaggtttc gtcaccgttg cagtagtcca tagtcatcgc tgacttttcc
    28021 gtcataaacg cgagtttttc atgggtgatc aggcgttcta cgggggcgga atcggcgaaa
    28081 cctggaataa tgtgttccag actgtgggca tagagacgcc cgccagtaac gttcttggca
    28141 cctgcggaat tgccacgctc gataactaac acttgcgccc cttcgcgggc gagcaccagt
    28201 gcggcaaccg aaccggcaag ccctgcaccg acgatgatgg cgtcaaagat atcttcggac
    28261 ataagagctc cctgtcagcg gcagagccgt aatgcagccc tgccagagtg gatcaacgcg
    28321 ctaaagctgc ggtcagtgcc ggaaggatct tcacggcatc gccaacaatg ccgtaatccg
    28381 cgtactggaa gatcggcgca tttttatctt tattgatggc gaaaatggtt tgcgacgcgt
    28441 tagcgccaac catgtgctgg atctacccgg agatccccac cgccaggtac agttcaggtt
    28501 tcagcatcag gttggagata ccgacatagc gttcgtgctc catccatttt tcgttttctg
    28561 ccaccggacg agaacaggcc aactccgcac ctatcgcctt gcaaagctgt tctgccagcg
    28621 caatgttctc tttgctgcca atgccgcgac cgacgctgac caccagacgg gctttgtcga
    28681 gatcgacgct gttgctctgg cgcgcctggg ttgccgtgcg ggtaatcgcc acagccggag
    28741 cctgccactc cacggtgtgc gtttcgccag tgcgtgacgc gtctggctga gccgcatcga
    28801 acgtgccgct gctgatggtc agtaccgcat acggcgtggc aatgcgttct tcgccaatcg
    28861 ccagaccgcc gtaaaccatg tgtttcactg ttgctttacc gtcctgtacg ctgacggtgc
    28921 tggcatcgtt agacaccgcc gctttaaggc gataacccag ttttgccgcc agtaatttgc
    28981 cgcgacgggt gtttggcaac agcaccagac cgtcagcgcc gtgctggcga atagtgtcag
    29041 ccatgacacc ggcgtaatct tcgatcatcc gatcgtccgg tttgccgttt aatttccaga
    29101 catgattagc gccgagctgg attgcctgtg cgccgtcggc atcattgagg acaaaggcat
    29161 tgatttgatt agctaaagcc tgcgcaccgt tcatcagttc cggcagacga gagggggtat
    29221 cgctgaatac ccagacttga gaaaacgtgt tcatagcatc ccctgtaatt aaatgacttt
    29281 gcgaagattt tcagcaaatg cggcgatctg ttcttcgccg tcgccttcaa tcacgatgcg
    29341 ctgacgctcg cgctgtttcg gcgcggcaac ctgttgttca gaccaggcgt ccacggcgtt
    29401 aaaaccaata tccgccgccg accatacctg gactggcttt ttcgccgcgc cgagaatggc
    29461 tttcatcgaa ggaatttgtg gggagttgat atcagtggaa acagcaacaa ccgcaggcag
    29521 cggaatgctt aaggtttcgg tttcatcttc cagttcgcgc tcaacggtga gggtatctgc
    29581 cgtcagggag ataattttgc tgacgccgtt aactgccgga atattgagga tttcgcccac
    29641 cagcagacca acctgctggg cataaaggtc ggaagaacca tcgccacaga ggatcagatc
    29701 aaagcctgct ttctgggcgg ctgcagccag tgcgctcgcc gtttgttgcg gcagtgcctg
    29761 ctcgaactgg tcatcaatca ccacaatcag ttcatccggg ccgcgcgata gcacatcttt
    29821 acgccctttg gcgttggtca gggctttacc gcccacactt aaggctgtca cctgcgcctc
    29881 tgctgcctgt tgctttagct ggcaagccgc ttcaatagcg ttgagatcgt attggcttat
    29941 tttggcatcg gctttgctga agtctaatga accatcagca ttattgaccg caatatcctg
    30001 ttcatcaggc acgcacttat agcaagtaat aatcttcatt gcatctccag aaatcatgaa
    30061 ggaaacgtcg ttcatatcgg tggtgttaat tacagatatc acgcccttta atttggaaat
    30121 aaaactttaa tcaccaatat tgaaagtcag atgctatttt tttacccaca aaagaatata
    30181 acaaactgat taataaccaa aattaacgaa aaacgccctc tgttcactcg aaacaaaaat
    30241 gtgataccaa tcacagaata cagcttattg aatacccatt atgagttagc cattaacgcg
    30301 tccacgaggt taataataat tatattaaat gttaacaaaa ataaaacaaa cgggaacgca
    30361 aaataaatat tcgttttcac agtggaatta actcatgaag aatgaaaaga gaaaaacggg
    30421 aatagaaccg aaggttttct ttccgccatt aataatcgtc ggcatacttt gttggcttac
    30481 agtcagagat ctggatgcgg cgaatgtcgt tattaatgct gtattcagtt acgtcaccaa
    30541 tgtatgggga tgggcatttg aatggtatat ggtggtgatg cttttcggtt ggttctggct
    30601 ggtgtttggc ccgtatgcca aaaagcgttt aggtaacgaa ccgccagaat ttagcaccgc
    30661 cagttggatc tttatgatgt tcgcctcctg tacgtctgct gccgtactgt tctggggatc
    30721 gattgagatc tactactaca tctccacccc gccgtttggc ttagaaccga actcgacagg
    30781 agcgaaagag ttggggctgg cttacagctt gttccactgg ggacctctgc cgtgggccac
    30841 ttacagcttc ctttcagtcg ccttcgctta cttcttcttt gtccgcaaaa tggaagtgat
    30901 tcgccccagc tcgacactgg tgccgctggc aggtgaaaaa cacgccaaag ggttgttcgg
    30961 taccatcgtc gacaacttct atctcgtcgc cttgatcttc gcgatgggta ccagtctggg
    31021 ccttgccacg ccgctggtga ccgagtgtat gcaatggttg tttggcattc cgcataccct
    31081 gcaactggac gctatcatca ttacctgctg gattatcctc aacgccattt gcgtcgcttg
    31141 cggtctgcaa aaaggggtac gtatcgccag tgacgtgcgt agttacctga gcttcctgat
    31201 gctgggttgg gtgttcatcg tcagcggtgc cagcttcatc atgaactact tcaccgattc
    31261 ggtggggatg ttgctgatgt atctgccgcg catgttgttc tataccgatc ccatcgctaa
    31321 aggcggcttc ccgcagggct ggaccgtgtt ctactgggca tggtgggtga tttacgccat
    31381 ccagatgagt atcttcctcg cccgcatctc ccgtggtcgt actgtgcgtg aactgtgctt
    31441 cggcatggtg atggggctga cagcatccac ctggatcctg tggaccgtgc tcggtagtaa
    31501 cactctgctg ttgatggata aaaacatcat caacattcca aatctgatcg aacagtacgg
    31561 tgtggcgcgc gccatcatcg aaacctgggc cgctctgcca ctcagcaccg ccaccatgtg
    31621 gggcttcttc atcctctgct ttattgccac cgttacgctg gttaacgcct gctcttatac
    31681 cctggcgatg tctacttgcc gcgaagtacg cgatggtgaa gaaccaccgc tgctggtgcg
    31741 tatcggttgg tcaattctgg ttggcattat cggtattgtt ctgctggcgc tcggcggcct
    31801 gaaaccgatt caaaccgcca ttatcgccgg aggatgcccg ctgttcttcg tcaacattat
    31861 ggtgacgctc tcctttatta aagacgcgaa acagaactgg aaagattaat taacccccaa
    31921 aatatcaaga ggttgaaaga tggattttaa tttaaatgat gagcaggaac tgtttgtcgc
    31981 cggtatccgc gaactgatgg ccagcgaaaa ctgggaggcc tattttgccg agtgcgaccg
    32041 tgacagcgtc tacccggaac gttttgtcaa agcactggcg gatatgggta tcgacagtct
    32101 gctgatccct gaagagcacg gtggtctgga cgcggggttt gttactctcg ccgccgtgtg
    32161 gatggagctg ggacgtctgg gggcaccaac ctatgtactg taccagctgc cgggcgggtt
    32221 caataccttc ctgcgcgaag gcacacaaga gcagatcgac aagattatgg ctttccgcgg
    32281 caccggtaag cagatgtgga actcagcgat taccgaaccg ggcgcgggct ccgacgtggg
    32341 tagcctgaaa acgacttata cccgtagaaa tggtaagatt tatcttaatg gtagtaagtg
    32401 ttttattacc agtagcgcct acaccccgta catcgtggtg atggcgcgcg acggggcttc
    32461 tccggacaaa cctgtctaca ccgaatggtt tgttgatatg agcaaaccgg gcatcaaagt
    32521 gaccaaactt gagaagctcg gtctgcgtat ggatagctgc tgtgaaatca cctttgacga
    32581 tgtggaactg gacgagaaag acatgttcgg tcgggaaggt aacggcttta accgcgtcaa
    32641 agaagagttc gaccatgaac gtttcctggt agccctgacc aactacggta cggcgatgtg
    32701 cgcctttgaa gatgcggcgc gctacgccaa ccagcgcgtg cagtttggcg aggctattgg
    32761 tcgtttccag ttgattcagg aaaaattcgc ccacatggcg atcaaattaa actccatgaa
    32821 aaacatgctg tatgaagcag cgtggaaagc agacaacggc accatcacct ctggcgatgc
    32881 agcgatgtgc aaatacttct gcgccaatgc ggcatttgaa gtggtggata gcgcaatgca
    32941 ggtgctgggc ggtgtcggga ttgcgggcaa ccaccgcatc agccgcttct ggcgtgacct
    33001 gcgtgtagac cgcgtttccg gaggatctga cgaaatgcag atcctgacgc tgggtcgtgc
    33061 ggtgctgaag caataccgct aagtccgtgc gctgcctgat gcgacgctga cgcgtcttat
    33121 caggcctacg ggagcacgaa tgtaagccgg ataaggcgtt tacgccgcat ccggcagtca
    33181 tgcgctacat aaatttttca ggagctaata atggatcatc tacccatgcc gaaattcgga
    33241 ccgctggccg gattgcgcgt tgtcttctcc ggtatcgaaa tcgccggacc gtttgccgga
    33301 caaatgttcg cagaatgggg cgcggaagtt atttggatcg agaacgtcgc ctgggccgac
    33361 accattcgcg ttcaaccgaa ctacccgcag ctctcccgcc gcaatttgca cgcgctgtcg
    33421 ttaaatattt tcaaagatga aggccgcgaa gcgtttctga aattaatgga aaccaccgat
    33481 atcttcatcg aagccagtaa aggtccggcc tttgcccgtc gtggcattac cgatgaagta
    33541 ctgtggcagc acaacccgaa actggttatc gctcacctgt ccggttttgg tcagtacggc
    33601 accgaggagt acaccaatct tccggcctat aacaccatcg cccaggcctt cagtggttac
    33661 ctgattcaga acggtgatgt tgaccagcca atgcctgcct tcccgtatac cgccgattac
    33721 ttttctggcc tgaccgccac cacggcggcg ctggcagcac tgcataaagt gcgtgaaacc
    33781 ggtaaaggcg aaagtatcga catcgccatg tatgaagtga tgctgcgtat gggccagtac
    33841 ttcatgatgg attacttcaa cggcggcgaa atgtgcccgc gcatgagcaa aggtaaagat
    33901 ccctactacg ccggttgcgg tctgtataaa tgtgccgacg gctacatcgt gatggaactg
    33961 gtgggcatta cccaaattga agagtgcttt aaagatattg gcctcgcaca tctactcggt
    34021 acgccggaaa tcccggaagg cactcagctt atccaccgta tcgaatgccc ttacggccca
    34081 ctggtagaag aaaaactcga tgcctggctg gcggcacata ccatcgcaga agttaaagaa
    34141 cgctttgccg aactgaatat cgcctgtgcc aaagtactga ccgtaccgga actggaaagc
    34201 aatccacagt atgttgcccg tgaatcaatc actcagtggc aaacgatgga tggtcgcacc
    34261 tgcaaagggc cgaacatcat gccgaaattc aaaaataacc ccggacaaat ctggcgcgga
    34321 atgccctcac atggcatgga cacggctgcc attttgaaaa atatcggcta cagcgaaaac
    34381 gacattcagg agttggtcag caaaggtctg gccaaagttg aggactaaac gcgtggcgtc
    34441 agtcggcttg cctgactagc gcccgcccaa taacaaacga acaaaaatgg atagaggtgc
    34501 aatggatatc attggcggac aacatctacg tcaaatgtgg gacgatctgg cggacgttta
    34561 cggtcataaa acggcgctga tttgtgaatc cagcggcgga gtcgttaacc ggtatagtta
    34621 tcttgagtta aatcaggaga ttaaccgcac ggcaaacctg ttttatacgc tggggattcg
    34681 caaaggcaac aaggttgcac tacatctcga caactgcccg gaatttatct tttgctggtt
    34741 cgggctggca aaaattggcg cgattatggt gccgattaac gcccgcctgt tacgcgagga
    34801 aagcgagtgg atcttgcaaa atagccaggc gtgcctgctg gtgaccagtg cgcaatttta
    34861 tcctatgtat caacagattc agcaggaaga tgccactcaa ttgcggcaca tttgcctgac
    34921 agatgtggca cttcccgctg atgatggagt gagttcattt actcaactga aaaatcaaca
    34981 acctgccacc ttgtgctatg caccgccgct atcgactgac gatacggcgg aaattctctt
    35041 cacctccggc accacctccc gaccgaaagg tgtggtgatt acccattaca acctgcgctt
    35101 cgctggatat tactccgcct ggcagtgtgc actgcgtgac gatgacgtct acatgacggt
    35161 aatgcctgcg tttcatatcg attgccagtg tactgcggcg atggcggcgt tttctgccgg
    35221 ggccaccttt gtgctggtcg agaaatacag cgcccgcgcc ttctggggac aggcgcagaa
    35281 gtaccgcgcc accattaccg aatgtattcc gatgatgatc cgtacgttga tggtgcagcc
    35341 gccttcagcg aacgatcggc aacaccgcct gcgggaagtg atgttttatc tcaacttgtc
    35401 ggagcaggaa aaagacgcat tttgtgaacg cttcggcgtt cgcttgctga cgtcttatgg
    35461 gatgacggaa accattgtgg gcattatcgg cgatcgccct agcgataaac gacgctggcc
    35521 gtcgattggt cgggcggggt tttgctacga agcggagatc cgcgacgatc acaatcgccc
    35581 gctcccggca ggtgagatcg gtgaaatctg tattaaaggc gtaccaggga aaaccatctt
    35641 caaagagtat tttctcaacc cgaaagccac tgcgaaagtg ctggaagccg acggctggct
    35701 acataccggc gataccggat accgcgacga agaaggcttt ttttatttcg tcgatcgccg
    35761 ctgcaatatg atcaaacgcg gtggcgagaa tgtctcctgc gtggagctgg aaaatatcat
    35821 cgccacccat ccgaaaattc aggacatcgt ggttgtgggt attaaagatt cgattcgcga
    35881 tgaagccatc aaagcatttg tggtgctgaa tgaaggtgaa acattgagcg aagaggaatt
    35941 ttttcgcttc tgcgaacaaa atatggcgaa atttaaagtg ccctcttatc tggagatcag
    36001 aaaagatctg ccacgtaatt gctcggggaa aataattaga aagaatctga aataaatgaa
    36061 acgtcaggga actaccctgc cagctaataa tcacgcccta aaacaatatg cattttttgc
    36121 agggatgttg tcgtccctaa aaaagcaaaa atggagaaaa ggaatgagtg aatcattaca
    36181 tctgacccgc aatggatcaa ttctggaaat tacccttgat cgcccaaaag cgaatgctat
    36241 tgatgcaaaa accagctttg aaatgggcga agtatttcta aatttccgtg acgatccgca
    36301 attacgtgtc gccattatta ccggtgccgg agagaaattc ttttccgcgg gctgggattt
    36361 aaaagcggca gcagaaggcg aagcaccgga tgctgacttt ggcccgggtg gttttgcggg
    36421 attaaccgaa attttcaatc tcgacaaacc agttatcgca gctgtgaacg gctacgcctt
    36481 tggcggcggt ttcgaactgg cgctggcggc agattttatt gtttgtgccg ataacgccag
    36541 cttcgccctg ccggaagcca aactgggcat cgttcctgac agcggcggtg tgctgcgtct
    36601 gccgaagatc ctgccgccta ccatcgtcaa tgaaatggtg atgaccggca gacgaatggg
    36661 cgcagaagag gcgctgcgtt gggggatagt caaccgcgtg gttagccagg cggaactgat
    36721 ggataacgcc cgcgaactgg ctcagcagct ggttaacagc gccccgctgg cgattgcggc
    36781 gctgaaagag atctaccgca ccaccagcga aatgccggta gaagaagcgt atcgctatat
    36841 tcgcagcggc gtgttgaaac actatccatc ggttctgcat tcggaagatg ccattgaagg
    36901 gccgctggcg tttgccgaga agcgcgatcc ggtgtggaaa ggacgttaac aaccgtgagc
    36961 tattacgcct ttgaggggtt aattccggtg gttcacccga cggcgtttgt ccatcccagt
    37021 gccgtcttga ttggcgatgt gattgtggga gccggtgtct acatcggccc actcgcctca
    37081 ctgcgtggtg actacgggcg gttgatcgtg caagcgggag ccaatattca ggatggctgc
    37141 attatgcatg gctactgcga cactgacacc atcgttgggg aaaacggcca tatcgggcat
    37201 ggagcgatcc tgcatggttg tgtgattggt cgcgatgcat tggtcgggat gaacagcgta
    37261 attatggatg gcgcggtcat tggcgaagag agcattgttg ccgcaatgag ctttgtcaaa
    37321 gcaggcttta gtggcgagaa acgccagttg ttgatgggta cgccagcccg cgctgtacgc
    37381 agtgttagtg acgacgagtt acactggaaa cggttgaata ccaaagagta tcaggatctt
    37441 gtcgggcgct gtcatgcatc gttacatgaa acgcagccac tgaggcaaat ggaggaaaat
    37501 cgcccccgtt tgcaggggac gacggatgtg acgcctaaac ggtgatcttg ccggatgcga
    37561 cctggttgct ggataagatg ctacaacatc gtatctggca agcaggacgc ccggtatcaa
    37621 cgagataaaa ttaacgacgc atactctttg acaacatcat ctgccacttc tgctcgcggt
    37681 taagttccgt gagcggaacg gcaggaatac gcggtgtctt tcttacgccg actaattttt
    37741 cccgactgtt attgcgcagg cgcgcgtaaa tttgctcatc gatatcgacc actttaacca
    37801 gtcgctgaca ctggcatccc cgcccttcca gcttattagg gatcatctta acttcgcagc
    37861 ttatttctgt gacttccgac agaatataag tcagggtatt aaccgccttg ctgtgttcaa
    37921 tatcgaaatg aatagagatc tctcttgcta tcacccaccg attttcagcc atcatccatt
    37981 cggcgattaa caagtagagt ggtttttcaa catatccttc acacatacga actccagttt
    38041 cacaaattaa aagtctagct atgactcgtt ttgaagcaat taaacaaggc catattaaaa
    38101 ttgtggatat atcgatagta tgcaacttca ccgtcgataa gtgtgagcta aatcctgctt
    38161 atgtcatcaa aaatatcgat tccccaaaag atttacttaa tgggcaaaaa aaaacggtcc
    38221 tcatcagaga accgtattag ttattcgatc aaattagcgg atgaaaaata tccgccacga
    38281 cacgctatta tttgatctgt gcgtgcattt cctgcaccga aattactttt tcagtcgcat
    38341 cggcattcag cgccatcgcg gtagcgaaac caccgttcag ggtggtgtca taatgcactt
    38401 tatattgcag cgcactgcga cggatcacgc gggagtcttc aatcgcacga cggcctgagg
    38461 tggtgttgat gatgtaggta tattcgccat tcttgatacg gtcctgaatg tgcggacggc
    38521 cttcatgcac cttgtttacc agacgcggat tgatacccgc ttcgcccagc acaatcgccg
    38581 tgccgtgggt cgcatccagc tcgaagccct gtttcagcag ttttgccgcc aggtccacca
    38641 cgcgttcttt atcgccttcg cgcacggaaa gcagcgcacg accgtgtttc ttcatggtgg
    38701 agttgctgcc cagctgcgct ttggcgaacg cttcagcgaa ggtgcggccc acgcccatga
    38761 cttccccggt agagcgcatt tctggcccta acagcgggtc aacgcctggg aatttattga
    38821 acggcagcac cacttctttc accgagtagt acggcggaat aacttctttg gttacgccct
    38881 gctcagccag cgatttgcca gccatcacac gcgccgccac ttttgccagc ggtacgccgg
    38941 tggctttgga gacgaacgga acagtacgcg ccgcacgcgg gtttacttca atcaggtaaa
    39001 cttcgttgtt tttcaccgca aactgcacgt tcatcagacc gcgcacctgc aattcgaagg
    39061 ccagtttctg cacctgctgg cgcatcacat cctgaatttc ctgacttaag gtgtacgctg
    39121 gcagcgaaca tgcggagtca ccggagtgca cacccgcctg ctcgatgtgc tccatgatgc
    39181 cgccaatcag caccatttcg ccgtcgcaga tggcatccac gtcaacttct actgcgtcat
    39241 caaggaaatg atccagcaac actggcgcat cgttagacac gctaaccgcc gtctggaagt
    39301 agcgacgcag gtcagcttcg tcatagacga tttccatcgc ccgaccgccg agaacgtaag
    39361 acggacgcac caccagcggg tagccaatct cttttgcctt ctcaactgcc atttcaatag
    39421 cagtaacggt ggcgttcgcc ggttgtttca gtttcagacg gtcaaccgca tgctggaagc
    39481 gttcacggtc ttccgcacgg tcgatagcat ccgggctggt gccgataacc ggtacgccag
    39541 cggcttccag tgcacgcgcc agtttcagcg gggtctgacc gccgtactga acgataacgc
    39601 ctttcggctt ctcaatacgc acgatttcca gcacatcttc cagagttacc ggctcgaagt
    39661 agaggcgatc ggaagtgtcg tagtcggtag agacggtttc cgggttacag ttaaccataa
    39721 tggtttcgta accgtcttca cgcagcgcca gcgaggcgtg tacgcagcag tagtcgaatt
    39781 cgataccctg accgatacgg tttggaccgc cgccaagcac catgattttt tcacggtcgg
    39841 tagacggatt cgcttcgcac tcttcttcat aagtggagta catgtaagcg gtgtcggtgg
    39901 caaactctgc cgcacaggta tccacgcgct tgtagaccgg gtgcaggtca tattggtcac
    39961 gcagcttacg gatttccgct tcgcgtacgc ccgccagttt tgccaagcgc gcatcggcaa
    40021 agcctttgcg tttcagctgg cgcaggaatt cagcgttcag gccagtgatg cccacttccg
    40081 ccactttctc ttccagacgc accagttctt caatctgtac caggaaccag cggtcaatgt
    40141 tagtcaggtt gaagacgccg tccacagaca ggcccgcgcg gaaagcatcg gcgatgtacc
    40201 agatacgctc tgcgcctgcg tctttcagtt cgcgacggat tttggttaac gcttccgggt
    40261 catccaggct cactttcggg tcgaagccag ttgcgcccac ttccaggccg cgcagcgctt
    40321 tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac ttcgccaacc gatttcatct
    40381 gagtggtcag acggtcgtta gcaccggcga atttttcgaa gttgaagcga ggaattttag
    40441 taaccacata gtcgatggac ggctcgaagg aggccggagt acgtccgcca gtgatgtcgt
    40501 tcatcagttc gtctagggtg taacccaccg ccagtttcgc cgccacttta gcaatcggga
    40561 aaccggtcgc tttcgacgcc agcgccgaag aacgggacac gcgtgggttc atttcgataa
    40621 caatcaggcg accgtttttc gggttcaccg caaactggac attggaacca ccggtttcaa
    40681 cgccgatttc acgcagcacc gccatcgagg cgttacgcat gatttgatat tctttgtcgg
    40741 tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt gtggatgccc atcgcatcaa
    40801 agttttcgat agagcagacg atgatgcagt tgtcgttttt atcacgcacc acttccatct
    40861 cgtactcttt ccagccgatc agcgactcat caatcagcaa ctctttggtc ggagagagat
    40921 ccagaccgcg ggcgcaaatt tcttcaaact cttcacggtt ataagcgata ccgccgccgc
    40981 taccgcccat ggtaaaggat gggcgaataa tgcacgggaa gcccacgtca gcggcaacag
    41041 ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc ggtttccaga ccaattttct
    41101 tcatcgctac gtcgaaacga cggcggtctt ctgctttatc aatcgcatcg gcagtggcac
    41161 caatcatggt gacaccgaac tcttccaaca cgccctgacg ttccagctcc agcgcgcagt
    41221 tcagcgccgt ctgaccgccc atggttggca gcaccgcgtc cgggcgctct ttttcaataa
    41281 tcttgcgtac cacttcccag tgaatcggct cgatgtaggt cgcatcggcc atttccgggt
    41341 cggtcatgat ggtcgccggg ttggagttca ccagaatgac gcggtaaccc tcttcgcgca
    41401 gggctttaca tgcttgcgcg ccagagtagt caaactcaca cgcctgaccg ataacaatcg
    41461 ggcccgcacc cagaatcagg atacttttta tatctgtacg ttttggcatg gctcttttac
    41521 tcctgattac ttagcggttt tacggtactg ctcaattaac ttgataaagt ggtcgaacaa
    41581 cggcgcggcg tcgtgtggac cagggctggc ttcagggtga ccctggaagc tgaacgccgg
    41641 cttatcgatg cgatgaatgc cctgtaacgt accgtcgaac agggatttat gcgtgacacg
    41701 caggtttgca ggtaatgttg cttcatccac cgcaaaaccg tggttctggg cggtgatcat
    41761 caccacgttt ttctccacat ctttaaccgg atggttgccg ccgtggtgac caaatttcat
    41821 tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga tgaccgagac agatgccgaa
    41881 taccggaata tcggtttcga ggaacttctg gatggcggta atggcgtaat cgcacggggc
    41941 cgggtcgcca ggaccgttgg agaggaagat gccgtctgga ttcattttca gcacatcttc
    42001 cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct ctatccacca gcatccgcag
    42061 gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg aacggcagct cgtcttcttt
    42121 tttcgcttct ggcaggccgc cggtcaacgt ccagctccct tgtgtccagc tataggcttc
    42181 tgcggtggtc acttcttttg ccagatccat gccgttcaga cctgggaacg cgcgggcttt
    42241 ttctaacgcc agcgccgcat ccgggttatc gcctgcgata atgcagccat tctgtgcgcc
    42301 tttctcgcgc agtaaacgcg tcagcttacg ggtatcgata tcggcaatcg ccacgatgtt
    42361 atggcgcttc aggtaagaag agaggtcttc ggtattacgg aagttgctgg caatcagcgg
    42421 caggtcgcga atcaccagac cttgtgcatg tacctgagaa gattcttcat cggcgtcatt
    42481 agtgccgaca ttgccaatat ggggataagt aagagtaacg atttgacgag aataggaagg
    42541 atcagtgagg atttcttgat aaccggtcat tgaagtattg aaaacgactt ccccaaccgc
    42601 cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt ccgtcttcca gaaccaatag
    42661 cgctgactta atcaaaacac cctccagaga atactcactc actttatttg catattaatt
    42721 cacagtgatg acgtaaatca atgcaaatct gcttaccgac gaatttctgg caaacggcgg
    42781 cattctggag atatgggcgc taaaagtcaa cttaatggca ggatttttta tattattttg
    42841 tggctcatgc atgttttaca ggcattaagc gacaaaaaca tcatctaacc tgttctggaa
    42901 aacgcttgcg cagcaagaag attgcatgaa ataaaaacga gcaggaaaaa gtggaccaaa
    42961 tggtcaaaat ttacattaaa acaacaaaaa tagaagacga aacatgtaat ttttggcagt
    43021 tatagaaaat ttaaaagcat aacaccaaaa ataaaagggc taaaaattaa agccctttat
    43081 aattaataaa ttaaatgtgt tacttttgca tcataataaa cattttgtgg ttacaaatta
    43141 ttgagatcaa gcacatctcg catatcaaaa agaccacttt ccttaccact caaccacaaa
    43201 gccgatctta ccgcaccgtt agcaaatgtc atgcggctgg acgccttatg ggtgatctcc
    43261 agacgctcgc caatatcggc aaacatcgcg gtatgttcgc caacgatgtc acctgcacgc
    43321 acggtagcaa aaccaatggt acctggcaca cgttcaccgg tatggccttc acgactgtag
    43381 accgcgcaat ctttcagatc tttatcaagg gcgtgggcga tcgcctctcc cattgccagt
    43441 gcggtgcctg acggcgcatc aactttatgt ctatgatgtg cttcaataat ttcgatatcg
    43501 gtgtagtcac ccatcacttt ggctgctttc tccagcagct taagcatgac gttaacgcca
    43561 acgctaaaat tggcggcaaa gacaatcgca atatcggcgg cggcgtcacg aattgcctgt
    43621 ttaccggctt cgtcaaaccc cgtagtgccg atcaccatcc ctttgccatg ctgccgacaa
    43681 aaagcgagat ggttcagcgt accttccgga cgggtaaaat cgataaacac atcaaaatca
    43741 tcttttatcg catcaagact gctttgcacg gtaacgcctg ttttccctgc accagccagc
    43801 tcaccggcgt cgctgcccag taaagaagat ccttcgcgct ccagcgccgc gcccagctgc
    43861 acgccttcta atgccagtgc cgcctgaatc aactggcggc ccatacgccc cccggctccc
    43921 gcgatggcaa cgcggatgtt tgcatcatgc atagctattc tcttttgtta atttgcatag
    43981 accgttttca gagtaaccag cgcatcgcta cgccgccagc caaaaacgct gataattaga
    44041 aattaatgat aaacagggag ggatatcagt gaaaggcatg agtcgatgaa tgactgcatg
    44101 ccgataacat gtgacaggtt acggcaccag agccagcacc tcagccaccc actgttggaa
    44161 gccttttaca ttcagatcca atgccacctg tacattggct ggtttgccca ggcaaccgtc
    44221 gatatcaacc accgtcgtgc ctgaggtaaa ttcgccttga gtttctaccg cgacaaaaca
    44281 cggcttgagg gtgaataagt ccgggcgcac cagccaggcg atggcgcaga gatcgtgcat
    44341 tcgcagaccg ctttgcatac tgccgctacg gtagtggcta aacagggcgt ggagcatttt
    44401 tccggtgcga ttcaactccg gtaaagtagc gagatagtca ggggttaata ttgcctgatt
    44461 ggtgacatcc agaccgcaca tgacaatttc gatgccgcta cggaagacac aggcagccgc
    44521 ttctggatcg gcagcgatat taaactcggc atttggtgta cagttgccgc gaccagcaga
    44581 accgcccatg atcaccagac gacgaatata cggtttacat tccgggcatt gtaaaagtaa
    44641 cagcgcaata ttggttaacg ggccgatggc caccagggta acgggctctg gtgcacgcat
    44701 cagggcatcc cgaatcgcca gaaacgccgg tatcccgagt ggctttcggt tgtgctcaac
    44761 aaagtcgtag ccagccattc ccgattcgcc gtgcacagat gctgcatcac gcggtgcgcg
    44821 taccagcggc acagcggctc cctgggcgag cggaatctcc gcattccaga aatgcagcag
    44881 ttgcagggca ttgcgggtag ttttctcaac cgagacatta cccgcgacgg tggtcatcag
    44941 ttgcaggtcg agttcggggg caaaaatcgc ggcggcaatg gcgacggcat cgtcaatgcc
    45001 ggggtcagta tcgaggaaga taggtaaacg catgttttct tcataaaaaa tgccggtaac
    45061 aagaccggca ttttcgcata acttaggctg ctaatgactt aatcgacttc acgaatatcg
    45121 acacgcagct ctttcggcac ttcgaaaaca atattttctt cacggccttc cagcggaatg
    45181 gcttcaccac cacccaactg ctgcaaacgt gccaccacat tctgcaccag aatatccgga
    45241 gccgatgcgc ccgcagtcac gccgacgcat ttaacctctt tcacccactc ttcctggata
    45301 tctttcgcat cgtcaatcaa aaacgcgtgt ttgcccatac gctgggccag ctccgccaga
    45361 cggttggagt tggaggagtt tttcgaaccg accaccaaca caacttccgc ctgttctgcc
    45421 agggcgcgta ccgcttcctg acggttagtc gtggcgtagc agatgtcatc tttgcgcgga
    45481 ccgacaattt tcgggaagcg tttacgcagc gcgtcgatca catcagacgt gtcatccacc
    45541 gacagcgtgg tttgggtcat aaaggagagc ttctcttcgt ttttgaccgt cagtttccac
    45601 acatcgtctg gcgattcgac tagatacatt cccccttcgg ggttgctgta ctgccccatc
    45661 gtcccttcca cttccgggtg cccggcgtgg ccgatgagaa tagattcttc gccacggcga
    45721 ctggcgcggg caacttccat atgcactttg gtcaccagcg gacaggtggc atcaaacacc
    45781 gtcaaatcgc ggcttttcgc ttcgttacga accgcctgag aaacaccgtg tgcggagaaa
    45841 atcaggatcg cgccgtccgg cacttcgcta atctgctcaa taaagatagc cccacgctcg
    45901 cgcaagctat cgaccacgta gcggttatgc accacttcgt gacggacata tatcggtgcg
    45961 ccgtaaatgg ccagcgcgtt ttcaacaatg ctgatagcgc ggtctacccc ggcacaaaaa
    46021 ccacgcgggt tggccaacag gatctgcatg ttacgcctcc agtgccggat cgatttccag
    46081 cacttcaata tcaaaatgaa cggtctgccc ggccagcgga tggttgaaat caacggtaat
    46141 ggagtcgccg ttaatttcgc ggatcacacc aggcatctca ctgccatcca ttgcggtaaa
    46201 aagcatgatt gcgccaattt ctggctcgcc tgcatccata aattcacggc gggagaagta
    46261 ctgaatcagg tccggtgacg gcacgccaaa cgccgcatct ggctccagcg agaaggtggt
    46321 tttgtcgccc actttcagcc ccagcaggtg ttgctccagc ccttcagaaa gagaagcatc
    46381 gcccaggcgg aacagcgccg gtttaccgtt gttgcgagta gactcggcgg tggtgccatc
    46441 gtcgagtttt agcgtgaagt gcaccaggac ggcgctattg ctctgtacag attcagacat
    46501 gcaggtttgc tcttattttt gaggattttt gccggatgcg gcctacgatt tcgtagcaat
    46561 ctgtaggccg catccggcag gatttattat tgttttttcg ctttggaagg taaaaaccct
    46621 tccagcacga tcagtgccgc accgacacaa atggcagtat cggcaaggtt gaaggtggcg
    46681 aagtgccagt cgccgacgta gaagtcgatc atatcgacaa cgaagccgtg ccacaggcgg
    46741 tcgaacaggt tgcccagcgc gccgccaata atcagcgcgt aagcgatatt gtttagcttc
    46801 tgcgtggcct tcgagcgata catcatcact gccaggagca cgctaatacc aatcgcaata
    46861 ccggcaaaga accaacgctg ccagccgccg ctatcggcaa ggaaactaaa cgccgcgcca
    46921 tagttacgcg cataatgcag attaagcgac gggaacagcg ggaccgtatc ccccagagca
    46981 aagttctgga ggatcaggta tttgctgccc agatcgataa tcagcacgac taccaccagc
    47041 cacagccagc gtagccctgt tgaacagatc gattgactca tcaggcaaac ttacgttttt
    47101 caccgtcacc ggcgacgttg ctgacacagc ggccgcagat ttctgcgtgt tccgccacct
    47161 tgccgacatc ctgggtgtag tgccagcagc gtgggcactt ctcaccttcg gctttactca
    47221 acgcgacttt cagccctttg agtacttcgc tctgctgagc atcagcaggt gcgtcgttat
    47281 agtctgcaac ggtagcgccg gaggtcaaca ggacaaatcg taattcatcg cccagcgcgg
    47341 tcagtttcgc cgccagttcc ggttctgcat acaaggttac tgccgcttcc agcgagccac
    47401 ccactttctt gtcggcacgc gcttgctcaa tgactttgtt cacttcgcca cgcactttca
    47461 acagctcgtc ccagaacgca tcgttcattg cttcactgtc tgccagacca aacaggcctt
    47521 cgtaccactc accggtgaag acgtattttt cacgttcgcc cggcagatag ccccacactt
    47581 catcagcggt gaaggagagg attggtgcca tccagcgcac cagcgcttca gcgatgtgat
    47641 acagcgcagt ctggcagcta cgacgcgcca cgctatccgc tttggcggtg tactgacggt
    47701 ctttgatgat gtcgaggtag aaggaaccca tctcaacgga gcagaagcgc atcagacgct
    47761 gtaccacttc gtggaaatcg tatgcttcgt acgccttgag gatgtcttcc tgtgccgctt
    47821 tcgcacaacc tacggcccag cgatccagta ccaccatctc ttccggtttc accatatctt
    47881 ttgctggatc aaaaccattc aggtttgcca gcaggaagcg cgcggtgtta cggatacgac
    47941 gatagctatc ggcagcacgt ttcaggatct cgtcagaaac ggccatttca ccggtgtagt
    48001 cggttgatgc cacccacaga cgcagaatat ccgcgcccag tttgttcatc acatcctgcg
    48061 gcgaaacggt gttgccgatg gatttagaca tcttgcgacc ctgaccatcc acggtaaagc
    48121 cgtgggtcag tacctgacga tacggcgctt tgcctttcat cgcggtggag atcattaggg
    48181 aagacatgaa ccaaccacgg tgctggtcag aaccttccag atacatgtcc gctgcgtgac
    48241 cggcaaattc cggacgcacg tcaacaacag aagagtgggt agatccggag tcaaaccata
    48301 cgtcgagagt atccggcact ttcacatact ggtcagcttc gtcgccgagg atctctttcg
    48361 catcgagatc ccaccacgcc tggatgccgt caacttcaac gcgtttagcc acttcttcca
    48421 tcagttcgag ggtacgcgga tgcagctctt ccgtgtcttt gtgcacgaac agtgacatcg
    48481 gtacgcccca ggtgcgctga cgggagatac accagtcagg acggttagca accatcgact
    48541 cgatacgcgc ctggccccag tccgggagcc actgcacgcc tttgatctct ttcagtgact
    48601 gcgcacgcag acctttctga tccatgctga cgaaccactg cggtgtcgcg cggaagatga
    48661 tcggcgtttt gtgacgccag cagcacggat agctgtgctg cattttctca acgtgcagca
    48721 gcgcgccttt ttcctgcagc agcgcaacga cgatgtcgtt cgctttgaag acgttcacgc
    48781 catccagcgt cggataagtg cccggcagat aagtgccgtc cgggccaacc gggttagcgg
    48841 tttccaggcc gtatttctga ccgatcacat agtcgtccgg gccgtggcca ggcgcggtgt
    48901 gaacggcacc ggtaccggca tccagggtaa cgtgatcgcc gaggattgcc ggaacgtcga
    48961 agcccataaa cggatgggta aagcgcagca gctcaagctc tgcacctttt accgtgccga
    49021 gaatggtgta atcggtcacg ccgatacgct gcattacgct ttcaaccaga tctttcgcca
    49081 gaatcacggc ctgaccgtcg atctgcacca gcgcatagtc gaaatcaggt gcaatagaga
    49141 ttgcgcggtt cgcaggcaga gtccacggcg tagtggtcca gatcaccagc gagattgggc
    49201 cgttaacgtt gcttacgcca aatttcgctt tcagcgcatc ctgatcgacc gcctggaaag
    49261 cgacgtcaat ggacggagaa gttttgtcgt aatactcaac ttccgcttct gccagtgcag
    49321 aacggcagtc tacgcaccag tgcaccggct tcgcgccttt gtgcaggtga ccattgccga
    49381 tgattttgcc cagcgcgcgg atgatgttgg cttcagtttt gaagtccata gtcagatacg
    49441 ggtgcgacca gtcgcccagc acgcccagac ggataaagtc tttgcgttga ccgtcaacct
    49501 gggtcgccgc gtattcgcgg cacttggcgc ggaactcggc ggcggtgaat ttctcacccg
    49561 gcttaccgta ttcttgctct actttcagct cgatcggcag accgtggcag tcccagccag
    49621 gcacatacgg cgagtcataa ccggaaagcc ctttggactt cacgataatg tctttcagaa
    49681 tcttgttaac cgagtgacca atatgaatgc tgccattcgc ataaggaggg ccatcatgca
    49741 gaatgaaggt ttttttgcct tttttagccg cacggatgat gccgtacaga tcatcatcag
    49801 tccaacgcgc cagcattccc ggttcgcgct tggcgagatc gccacgcatc gggaaccctg
    49861 tttccggcaa attcagggtt gatttatagt cactcatcag attctcggtt ccgtgtttcg
    49921 gtttgattac ataacaggct taagccggtt ttgttagccc aaaaaattcg cgggcggtta
    49981 attcatcacg cgcaatctgc gctttcagtt cgtccagcga cgcaaatcgc tgctcattgc
    50041 gtattttttt acgcagcact acttgtatat ggcgaccgta aaggtccatt gcaacatcta
    50101 acaaatgcac ttccagctgc tggcgaatac cggcaaccgt tgggcgtgtt ccgatgtttg
    50161 ccacgccggg taacggcttt tcaccgaggc ccagcacttc taccgcataa acccctttca
    50221 ccggggaaac ctgacggcgt agcggtacat tcgccgtcgg gaaacctata gtgcgcccta
    50281 attcatcacc gtggactaca cgcccggaga tggcaaacgg gtgccccagt aaactttctg
    50341 ccagagccag attgtcatca gccagcgcct gacgcacggc ggtgctgctg atacgcacac
    50401 caccttcgca aaaagtttgc gtactggtga tatcgaagcc gtattccatg ccagctttct
    50461 gtaataacaa gaaatcgcct tcacgaccag cgccaaagcg gaaatcatca cctacggcaa
    50521 gaaattttac gcgcaaatgc ttcaccaaaa gatcgctgat gaaattttgc gcggttaacg
    50581 ccgcaaaacg cctgtcgaaa cgcacgcaca gcacgtaatc aacgccacac tctgcaaggt
    50641 aacgcagttt ttcccgcagc cgggtcagtc ttgccggggc tttatcggta gcaaacagtt
    50701 ccagtggttg aggttcaaaa agcatcacca tcaccggtaa gttgcgcttg cgcccttctt
    50761 cctgcaagcc ctgtaacagc gcgcgatgac cgcgatgcac gccgtcgaaa ttaccaatag
    50821 tcagcacaca cccttcttgc ggggcctggc tgagattatg tatgccgcgt atcagcttca
    50881 tgtctggctc aaaacagtga aaatcgtccg agtatacctt gtacagcggt caaggttaac
    50941 cggcgattga gtaccgaaat cagaaagaag tggcttttca ttgccatgga gcaaatcacg
    51001 ggaagaaact gaccgcctgc tgcaattttt atcgcggaaa agctgtattc acaccccgca
    51061 agctggtaga atcctgcgcc atcactacgt aacgagtgcc ggcacattaa cggcgcttat
    51121 ttgcacaaat ccattgacaa aagaaggcta aaagggcata ttcctcggcc tttgaattgt
    51181 ccatatagaa cacatttggg agttggacct tggctaatat caaatcagct aagaagcgcg
    51241 ccattcagtc tgaaaaggct cgtaagcaca acgcaagccg tcgctctatg atgcgtactt
    51301 tcatcaagaa agtatacgca gctatcgaag ctggcgacaa agctgctgca cagaaagcat
    51361 ttaacgaaat gcaaccgatc gtggaccgtc aggctgctaa aggtctgatc cacaaaaaca
    51421 aagctgcacg tcataaggct aacctgactg cacagatcaa caaactggct taatcgccaa
    51481 tttgctgaag ctttgtgaaa aagcccgcgc aagcgggttt ttttatgcct gctgcttttg
    51541 tgatgcgtga gagaacacac tgccggttgc cagatagtca aaagagacat gccgttgtac
    51601 tatctgaact cttcgtggct taagaatcgt agaaccatcg gctgatacat tctgaacaag
    51661 aatatctact caatctccag actcccccac aaagaatatg gatattgtga tacacattga
    51721 ggtattaaac ttaagaccac tctatttttg aattaaattc ctatatactt taaccattca
    51781 taaatatctc ctcattagac ctt
//
LOCUS       DADLTY010000005        49793 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.005, whole genome shotgun sequence.
ACCESSION   DADLTY010000005 DADLTY010000000
VERSION     DADLTY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.005"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..640)
                     /locus_tag="JN334_000249"
     CDS             complement(<1..640)
                     /locus_tag="JN334_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY7436523.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKIL"
     gene            814..1506
                     /locus_tag="JN334_000250"
     CDS             814..1506
                     /locus_tag="JN334_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY7436524.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(1555..3054)
                     /gene="pitB"
                     /locus_tag="JN334_000251"
     CDS             complement(1555..3054)
                     /gene="pitB"
                     /locus_tag="JN334_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HAY7436525.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(3346..5205)
                     /gene="gss"
                     /locus_tag="JN334_000252"
     CDS             complement(3346..5205)
                     /gene="gss"
                     /locus_tag="JN334_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HAY7436526.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            5410..6276
                     /gene="yghU"
                     /locus_tag="JN334_000253"
     CDS             5410..6276
                     /gene="yghU"
                     /locus_tag="JN334_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HAY7436527.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(6399..6647)
                     /gene="hybG"
                     /locus_tag="JN334_000254"
     CDS             complement(6399..6647)
                     /gene="hybG"
                     /locus_tag="JN334_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HAY7436528.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(6781..7122)
                     /gene="hypA"
                     /locus_tag="JN334_000255"
     CDS             complement(6781..7122)
                     /gene="hypA"
                     /locus_tag="JN334_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY7436529.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(7115..7603)
                     /gene="hybE"
                     /locus_tag="JN334_000256"
     CDS             complement(7115..7603)
                     /gene="hybE"
                     /locus_tag="JN334_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HAY7436530.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(7596..8090)
                     /locus_tag="JN334_000257"
     CDS             complement(7596..8090)
                     /locus_tag="JN334_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HAY7436531.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(8090..9793)
                     /gene="hybC"
                     /locus_tag="JN334_000258"
     CDS             complement(8090..9793)
                     /gene="hybC"
                     /locus_tag="JN334_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HAY7436532.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(9790..10968)
                     /gene="hybB"
                     /locus_tag="JN334_000259"
     CDS             complement(9790..10968)
                     /gene="hybB"
                     /locus_tag="JN334_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HAY7436533.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(10958..11944)
                     /gene="hybA"
                     /locus_tag="JN334_000260"
     CDS             complement(10958..11944)
                     /gene="hybA"
                     /locus_tag="JN334_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HAY7436534.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(11947..13065)
                     /gene="hybO"
                     /locus_tag="JN334_000261"
     CDS             complement(11947..13065)
                     /gene="hybO"
                     /locus_tag="JN334_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HAY7436535.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(13254..13541)
                     /locus_tag="JN334_000262"
     CDS             complement(13254..13541)
                     /locus_tag="JN334_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HAY7436536.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(13660..14586)
                     /locus_tag="JN334_000263"
     CDS             complement(13660..14586)
                     /locus_tag="JN334_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708817.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY7436537.1"
                     /translation="MLCLKKHQLRNATMPRLTAKDFPQELLDYYDYYVHGKISKREFL
                     NLAAKYAVGGMTALALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLV
                     KPAKMSGKTPAVGVVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKG
                     RELQQQVDPTKLMNDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACA
                     VPFYGRQAPTADVAKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVN
                     HGFHNDSTPRYDKSAADLAWQRTLKWFDKYLS"
     gene            14704..15744
                     /gene="gpr"
                     /locus_tag="JN334_000264"
     CDS             14704..15744
                     /gene="gpr"
                     /locus_tag="JN334_000264"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAY7436538.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(15784..16278)
                     /locus_tag="JN334_000265"
     CDS             complement(15784..16278)
                     /locus_tag="JN334_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAY7436539.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            16469..17353
                     /gene="yghA"
                     /locus_tag="JN334_000266"
     CDS             16469..17353
                     /gene="yghA"
                     /locus_tag="JN334_000266"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY7436540.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(17625..18050)
                     /gene="exbD"
                     /locus_tag="JN334_000267"
     CDS             complement(17625..18050)
                     /gene="exbD"
                     /locus_tag="JN334_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HAY7436541.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(18057..18791)
                     /gene="exbB"
                     /locus_tag="JN334_000268"
     CDS             complement(18057..18791)
                     /gene="exbB"
                     /locus_tag="JN334_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY7436542.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            19043..20230
                     /gene="metC"
                     /locus_tag="JN334_000269"
     CDS             19043..20230
                     /gene="metC"
                     /locus_tag="JN334_000269"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAY7436543.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            20370..21029
                     /locus_tag="JN334_000270"
     CDS             20370..21029
                     /locus_tag="JN334_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7436544.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(21069..21968)
                     /locus_tag="JN334_000271"
     CDS             complement(21069..21968)
                     /locus_tag="JN334_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7436545.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            22162..23325
                     /gene="yqhD"
                     /locus_tag="JN334_000272"
     CDS             22162..23325
                     /gene="yqhD"
                     /locus_tag="JN334_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAY7436546.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            23430..24257
                     /gene="dkgA"
                     /locus_tag="JN334_000273"
     CDS             23430..24257
                     /gene="dkgA"
                     /locus_tag="JN334_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HAY7436547.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            24457..25383
                     /locus_tag="JN334_000274"
     CDS             24457..25383
                     /locus_tag="JN334_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HAY7436548.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            25434..25691
                     /gene="yqhH"
                     /locus_tag="JN334_000275"
     CDS             25434..25691
                     /gene="yqhH"
                     /locus_tag="JN334_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HAY7436549.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(25734..27953)
                     /locus_tag="JN334_000276"
     CDS             complement(25734..27953)
                     /locus_tag="JN334_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAY7436550.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(28064..29476)
                     /gene="ftsP"
                     /locus_tag="JN334_000277"
     CDS             complement(28064..29476)
                     /gene="ftsP"
                     /locus_tag="JN334_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HAY7436551.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(29551..30288)
                     /gene="plsC"
                     /locus_tag="JN334_000278"
                     /gene_synonym="parF"
     CDS             complement(29551..30288)
                     /gene="plsC"
                     /locus_tag="JN334_000278"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HAY7436552.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(30522..32780)
                     /gene="parC"
                     /locus_tag="JN334_000279"
     CDS             complement(30522..32780)
                     /gene="parC"
                     /locus_tag="JN334_000279"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAY7436553.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMSTDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(33326..33808)
                     /locus_tag="JN334_000280"
     CDS             complement(33326..33808)
                     /locus_tag="JN334_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7436554.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(33861..34253)
                     /gene="ygiW"
                     /locus_tag="JN334_000281"
     CDS             complement(33861..34253)
                     /gene="ygiW"
                     /locus_tag="JN334_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HAY7436555.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            34405..35064
                     /gene="qseB"
                     /locus_tag="JN334_000282"
     CDS             34405..35064
                     /gene="qseB"
                     /locus_tag="JN334_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HAY7436556.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            35061..36335
                     /gene="qseC"
                     /locus_tag="JN334_000283"
     CDS             35061..36335
                     /gene="qseC"
                     /locus_tag="JN334_000283"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HAY7436557.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(36381..36713)
                     /locus_tag="JN334_000284"
     CDS             complement(36381..36713)
                     /locus_tag="JN334_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HAY7436558.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            37032..37613
                     /gene="mdaB"
                     /locus_tag="JN334_000285"
     CDS             37032..37613
                     /gene="mdaB"
                     /locus_tag="JN334_000285"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HAY7436559.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            37644..37958
                     /locus_tag="JN334_000286"
     CDS             37644..37958
                     /locus_tag="JN334_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY7436560.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(38006..39898)
                     /gene="parE"
                     /locus_tag="JN334_000287"
     CDS             complement(38006..39898)
                     /gene="parE"
                     /locus_tag="JN334_000287"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAY7436561.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(39927..40508)
                     /gene="yqiA"
                     /locus_tag="JN334_000288"
     CDS             complement(39927..40508)
                     /gene="yqiA"
                     /locus_tag="JN334_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HAY7436562.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(40508..41335)
                     /gene="cpdA"
                     /locus_tag="JN334_000289"
     CDS             complement(40508..41335)
                     /gene="cpdA"
                     /locus_tag="JN334_000289"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HAY7436563.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(41360..41782)
                     /locus_tag="JN334_000290"
     CDS             complement(41360..41782)
                     /locus_tag="JN334_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HAY7436564.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(41783..42412)
                     /gene="nudF"
                     /locus_tag="JN334_000291"
     CDS             complement(41783..42412)
                     /gene="nudF"
                     /locus_tag="JN334_000291"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HAY7436565.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            42617..44098
                     /gene="tolC"
                     /locus_tag="JN334_000292"
     CDS             42617..44098
                     /gene="tolC"
                     /locus_tag="JN334_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HAY7436566.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            44246..44917
                     /locus_tag="JN334_000293"
     CDS             44246..44917
                     /locus_tag="JN334_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HAY7436567.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            44923..46083
                     /locus_tag="JN334_000294"
     CDS             44923..46083
                     /locus_tag="JN334_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY7436568.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(46121..46936)
                     /gene="ygiD"
                     /locus_tag="JN334_000295"
     CDS             complement(46121..46936)
                     /gene="ygiD"
                     /locus_tag="JN334_000295"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HAY7436569.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLV
                     ELLAPIPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            47052..47825
                     /gene="zupT"
                     /locus_tag="JN334_000296"
     CDS             47052..47825
                     /gene="zupT"
                     /locus_tag="JN334_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAY7436570.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(47883..48053)
                     /locus_tag="JN334_000297"
     CDS             complement(47883..48053)
                     /locus_tag="JN334_000297"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436571.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(48314..48967)
                     /gene="ribB"
                     /locus_tag="JN334_000298"
     CDS             complement(48314..48967)
                     /gene="ribB"
                     /locus_tag="JN334_000298"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HAY7436572.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(49071..49219)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            49281..49631
                     /gene="ubiK"
                     /locus_tag="JN334_000299"
     CDS             49281..49631
                     /gene="ubiK"
                     /locus_tag="JN334_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HAY7436573.1"
                     /translation="MTYPYRTTMVLNTYQYRETTMIDPKKIEQIARQVHESMPKGIRE
                     FGEDVEKKIRQTLQAQLTRLDLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTE
                     IKKQPDPETLPPTL"
BASE COUNT        12483 a        13224 c        12336 g        11750 t
ORIGIN      
        1 caaggatctt ggcgccattg aatgtcagtt gcggcgtaac ggcgattttt tcctgtaacg
       61 ctgccagttg gtggtcaggt ttacgcgcaa acgcggtttg ttcatcaatg ccaagacgaa
      121 tcaacaatac gggtaaataa gacgccatcc attggtacag cttcaactcg cgctgcctta
      181 acatttttat ccaaccgtta ccgcccgtgg tttttgccaa ttgcgggcca tcaaagcgaa
      241 aacccggcac ttcaacctgc gggtagcggt cggtgatgag cagatagcct tgctggcttt
      301 tacacaacat tttgcgaaac ttgtacgccc gccagcagga aagcagatag ataaccagcg
      361 cagtaatatt gccaggcgga gttaagggct tttcgtgtac atgtgccgct ttacttcgca
      421 gataacaccc acaaggtgcg ccaataacag ggagctgtga aatccattcg ccaattcgcc
      481 cggacgattg cccgagataa atgtgttctg ttggcattct tgctgccagt tcatttacca
      541 ggcttgccgt gagggtcgat ttacctgaac catcacatcc tacaatggca ataacacgca
      601 ctggcgtgga attaattatt gacatattac gttgattcac gaaaaacccg gcagtaaata
      661 atgtattgaa tattacgtgg tccgtgctca gaatatccgt tcaaccttat tgtgacaatg
      721 actaattgtg acattgacct gaattacgct ttcataaaaa catattaacc aaataaatat
      781 ttttaatgga tatttaaatt aaaggatata ttcatgcagt caataacacc tccattaatt
      841 gccgttattg gtagcgatgc ttcaggcaag tcaacggtgt gtgaacatct tattaccgtt
      901 gtcgaaaaat atggtgctgc cgaaagagtt catttaggaa aacaggccgg aaatgtcggt
      961 cgtgcagtga caaaattacc tttgatggga aaatccttac ataaaacaat tgaacgaaat
     1021 caggtgaaaa cagcaaaaaa attgcctgga ccagttccgg cgctggtaat tacagcgttt
     1081 gtcgcccgtc gcttactgcg ctttcgtcat atgcttgcct gtcgtcgtcg cgggttaatt
     1141 gttctaaccg accgttatcc tcaggaccaa attcctggcg cttacgatgg tacggtgttc
     1201 ccacctaacg ttgaaggtgg tcgttttgtc tcatggctgg caagccagga acgtaaagcg
     1261 tttcactgga tggcgagcca taagcctgat ctggtcatca aactcaatgt tgaccttgaa
     1321 gttgcctgtg cacgtaaacc cgaccataaa cgggaatcgc tggcgaggaa gattgccata
     1381 acgccacagt taacctttgg tggtgcacaa ctggttgata tcgatgccaa tcagccactg
     1441 gaacaggtgt tggttgatgc agaaaaagcg attacggatt ttatgaccgc gcgtggttat
     1501 cactagtcaa aaatggaaat gcccgatcgc caggaccggg cattttcagg aaggttaaat
     1561 caactgcaat gctatccagt acagcccacc agaaagaaaa attgccgccg gtaaagtaaa
     1621 cacccacgcc atcaggatgc tggttaccgt tttacgctgt aacccaccgc cgtccaccac
     1681 catcgtccct gctaccgcag acgagaggac gtgtgttgtg gagacaggca tcccaatata
     1741 actggcaagg ccgatagaca ctgccgccgt catttgtgcc gccatgcctt gcgcatacgt
     1801 catgccgcgc ttaccaatct tctcaccgat ggtcatcgct acacgacgcc agccaatcat
     1861 ggtgccaatg ccgagcgcca gtgctaccgc catgatgatc cacaccggag cgtactcaat
     1921 ggtgcttaac atatcgctgc gcagtttttt cagcaggttc tggtcttctt tactgacgcc
     1981 tggcagtttc gccagcttcg cggaggtatc ggagatgcac agcataatgc ggcgcagctg
     2041 gctgcgctga ctcacgctta acggctcgta actttccata ttgcctggca gcatcgtttt
     2101 aacgcgcgca atcgcatcaa aggtatttgc cggatgacag tgaaactctg ttacttgcgt
     2161 gccatcagtc gatgctgcag gcaatggagg ttccatcgca atcaacttct gcggcagttc
     2221 aggatgctgt tgcaggtagt gttcgaagtt ggtaacggca tcgcgggtac gggtaatttc
     2281 atagccggac gcattcatat tgacgacgaa gccagcaggg gcaatcccca ccagtaccag
     2341 cattaccagg ccgatccctt tctgcccgtc gttcgcgccg tgcgaaaacg ccacgcccgc
     2401 agcggaaaca atcagcgcaa tacgcgtcca gaatggcggt ttacgtttgc cctttttctt
     2461 tttgcgatct tccggaatgc ggtgaatacg gtcacgcttt ttcgtcccac tccagaaacg
     2521 gcgcagcagg aaaatcagac cacccgcaat gaccaggccg acgatagggg aaacaatcag
     2581 cgaggagaaa attttggtta cttcacgcag gtttaacgca tccatcaccg atgagccggt
     2641 taacagcgcg ttggttaaac cgatgccgat aatcgcacca atcaaggtgt gcgaactgga
     2701 ggccggtaaa ccaaagaacc acgttcccag gttccagata atcgccgcca gcagcatgga
     2761 aaagaccatc gccaaaccgt gagttgaccc catattcagc aacaaatcga ttggtaacat
     2821 atggacaatg gcataggcaa cgctaagtcc gcccaataac acgccaaaaa agttaaaaaa
     2881 tgccgccatc accacagcaa gttgtggctg catggcacga gtataaataa cggttgccac
     2941 cgcattcgcc gtgtcatgaa aaccattgat tgcttcgtag aacaacacaa atgccagagc
     3001 aagtaataac aaaagccccg tatatatatc aaggccaaca aataaattta gcatatatta
     3061 gattacgcca ttttgaattt acgaacggac gcattatcag tgactttaac ggcatgggca
     3121 aagtgaaata tcatttttta acgataaaaa gagtgttatt tcttgtgtat cagacaataa
     3181 gcattcattc aaaaccttta gaaataatca aaacacaggt attccactgg ttaatatatt
     3241 cacatatgaa atgaataaat attggaatat ataaatattg aatattttga ttaatccccg
     3301 tactaattat tcttcataat cagtacgggg ttgctacaac atgtattact ttttcaccac
     3361 aattaacggt tcaatatcac tctctttttt gatgaccagt gattcatcac cgcgcaaaca
     3421 cgtcccaccg tagttgccgc caacggtgaa ggtacatacc tgaatgtatt taccgtccac
     3481 tttcggcaaa caccacagtt gctgatagat gtttttctgc tcggcaaatt taccgctggt
     3541 tttgtccagc acctcttcat gatggctgac caggtcgata ttgctaccac agcgaccggc
     3601 gatcggtttc actgcgtaac ctgttttcac caattcatca ttaacagtga aatcggtatc
     3661 cagcaggtaa cgatggtgcg ggaacagcga ccagaggatc ggcagaatcg ctttgttgcc
     3721 ggggatcacc gtccacagcg gctcaaagac cagcacttcc gggcgcagca atacgtcgat
     3781 aagacgcact tcgttttgcg gatgaccggt acggattggc accgcagcaa actcacggtc
     3841 gctaacttca cgaatctgat caaacgcggt ttcccacgcc caggttttcc acacgcagtt
     3901 aaccagtcgc ccttccccat caatcagttg cccggcagca tcccagccca gttcatccag
     3961 cccgcgcaag atacgcgttt caaagcccgc ctggtgcagc gcctgctcca taaactgcgc
     4021 gtgatagttt tcctcgatat ctttgtcctg catgatatgg acaaacggac gtgcacgact
     4081 gtgtttccag gcaccagcca gttcgttaat cagcccttcc gccggattga agccgttgcc
     4141 tttatagccc tgctccgccc aacgttcgag gatcaagccc gcttcggtat gacaggaggc
     4201 ggagtcggcg ttgtattcgt aaaccttcag gccacgctca tccatgcaga aatccatacg
     4261 accagtgatc atatggtgac ggcgacgctg ccaggagaga cgcaaacgtg gccagaggat
     4321 tttcggaatg tcgaacagcg ccagcaggtt gtcatctttc agcaccttgt cggttgcgtg
     4381 aagatacatc agatgcagct cgttggtggc tttaattagc tcctgctcgg cactctcggt
     4441 aatggtgtag taatgatacg gatcctgatt gatcacctga ccgttggcct gcacataggc
     4501 gttttgtagc ggatcttttt catccagcca tttaccgtca aactggcctt tgttttccag
     4561 acgcgctcca ctgattttca gcagctcgcc tgcaatttcc ggctgcggta agctgtattc
     4621 agtatcttcc gtctggatca tccagcccag aatggtggtg tcatcaaaag tgtctttaag
     4681 ggtatagcag ccgttttcga ccaccatctc cagctcgcgc gtccactgtt gcccttgcgg
     4741 caacggggaa tgaatcacgt tctgttccgc aatacggact ttgttgccat gcaattgggt
     4801 aatgatggcg acatggccag tgtctttaaa ttcaccgcct ttatcccaga taagaagcgc
     4861 acccgcgacc ggcgcacgcg gcgagccgtt aggaaatgcc tgcaatggca ggatgttgtc
     4921 attaaccact tctcgcagga agcgcagcga gaaaatctcc cacgccatac ccacgtcagt
     4981 aaagaccaca ccgtaattca gaaagagaaa acggcgagca aattcaacgc attgccactt
     5041 gtggcccata tattcgtcgt cgatatagct acggaatacg gcgtcatctt cgtattcctg
     5101 cggatcgaga gaactgtaat ctgaagagta gattgctacc ccacctgggg cgtagcccaa
     5161 taatgtcccg aacggggcat cctggctggt cgttcctttg ctcatcactt tacctttaac
     5221 aatacaacct aagcagttgg cgtaattgtt gtgctctgat tacctgctca gcgacattaa
     5281 ccggacagag gtcagactaa tcatacacct catcgcagcg gctggcgcaa agggttaaaa
     5341 aattcacatt ctgtacagca agtgacctgc tacactgctt caacactacc actcagaagg
     5401 caactcacta tgacagacaa tacttatcag cccgcgaaag tctggacgtg ggataaatcc
     5461 gctggcggcg cgttcgccaa tatcaatcgc ccgttttctg gtccgacgca tgaaaaaacg
     5521 ctgcccgttg gcaaacatcc attgcaactt tattcgctgg gaacgccgaa cggtcagaaa
     5581 gtaacgatta tgcttgagga gctactggcg ctgggcgtta ctggtgcaga gtacgacgcc
     5641 tggctgattc gtattggcga tggcgatcaa ttctccagcg gctttgtcga agtgaaccca
     5701 aactcgaaga tcccggcgct gcgcgatcat acgcataatc cgccgatccg cgtgtttgaa
     5761 tctggttcga tcctgcttta tctggcggag aaatttggct acttcctgcc gcaggatctg
     5821 gcaaaacgga ctgaaacgtt gaactggctg ttctggttac agggtgccgc gccgttcctc
     5881 ggcggtggtt ttggtcactt ttaccattac gcgccggtga aaattgagta cgccatcaac
     5941 cgctttacca tggaagccaa gcgcctgctc gacgtgctgg ataagcaact ggcgcagcat
     6001 aagtttgttg cgggcgatga gtacaccatt gcggatatgg cgatttggcc gtggtttggc
     6061 aacgtggtgc tcggcggcgt gtatgatgcc gcagagtttc tggatgcggg aagttataaa
     6121 catgtgcaac gctgggcgaa agaagtgggc gaacgtccgg cagtgaaacg tgggcgtatt
     6181 gttaaccgca ccaacgggcc gctgaacgag cagttgcatg agcgccatga cgccagtgat
     6241 tttgagacaa atacagaaga taagcgtcag gggtaagggt tggtgttcgt cgcagcaagc
     6301 catccaggcc ggataaggcg ttcacgccgc atccggcaat cgtgcacaat gcctgatgcg
     6361 acgctgccgc gtcttatcag gcctacaaaa gctaatcatc acgcgctggt aatgtcgtaa
     6421 tccatttggc gcagtgcgtc taatgtggct ttggcttcat cttcgtcgat gatgctcatg
     6481 gcaaatccga cgtgtaccag cacccactgg cccagcagat cggcagggtt accttcgcaa
     6541 atcagggcga tattcacgtc gcgcttgata ccacatactt caacctgcgc aagctggtga
     6601 atatcttcac cgacagccag cacctggcct ggaacgccaa tacacattgt taactccggt
     6661 tatttcgatt gccggacaaa cgcgaaaaag aacctttagc gggtgttccc tctcacccta
     6721 accctctccc cggaggggcg aggggaccgt ccgtgcgctt tttaggcgtt gtcctcagtc
     6781 ttattcaact tcaatacttt tgacgatcag cgaatcgccg gtatcgacac gcaaccgctc
     6841 gccgtgacag agcggacact gcgcatcgtg ctgatgaatc tccaccacct ggctgcaatc
     6901 ccagcaccag gcctgggcgg gtttatagac gatatgtaaa tcgcaccctt gcgccaccgt
     6961 tccgtggcag acaatttcaa aactaaaacg gacggcgctc tcctcaacgc aggagagcgc
     7021 gccaatttcc agccacacgg cggtgacgcg cttaacatcg tgctgctccg cctgccgttg
     7081 gataatttca acggcgctct ggcaaagcga caactcatgc attttcgccc ctcctgcggc
     7141 caaacagcag ggcgcgacgc cctgcgtgtg gtacatccgg attggtgact ggcaacgaaa
     7201 ggatcattcg tgcgcagtca tccgtcaggc gttggccttc ttcaatcgac atgctgtgcg
     7261 aaagcggcga catcagcgaa caggagaggt attgcgaaac accgtccagt tcgccaacgg
     7321 taaaggtcat ggtgccatac ggcagttgca gaccaatttt ttcactcact ttgcgcagcg
     7381 gccagagttg atccgggccg gggaagataa ctgcactcag catccacggg gtgatcacac
     7441 accccgtcca ctgaccttcg aacagcgtaa aatcagaaac atacaccggc attgaaggat
     7501 gcagaaaaga gagatcgtgc atcgaacgcc gggcaatttc ttcaaacgct gcctgtactt
     7561 gcgccttcgg ggaggtctgg aaacctgcta tctcttcagt catgaatcgc ctcccgtggg
     7621 atggcttcca cgcctgattc gcgcagcgca gccagaacct gctcaagcgc aggttcaatc
     7681 attgcttcaa ccgtcggcgt taagccgatg tgtggctcca gcgattccgg gatcacgccg
     7741 accagggtca gctttttcgg aaactcaccg gtgaagcgca gggccgacaa tacgtcggcc
     7801 aggccaagct gatgcggaga gattttgttg gtaaacaacg ccggaacttc ttcatcccgc
     7861 aggatcatca tcgttcccgg agtgttcttt ttcgacacaa tggcatccgc aataatcaga
     7921 tgatcgcgat ttgccatgtc gccaagcagc tccattcccg ccgtgccgcc atcgaggatc
     7981 tcaacataat ccggcagaat gtatcgttgc tctaacgctt cgacaatccg cacgccgatg
     8041 gcttcatcgg tcagcaaaat attgccgacc cctaagacta aaatacgcat tacagaacct
     8101 tcactgaaac cacttcgttg ccgtcggcat ccactacgtg taccgcacag gccatgcacg
     8161 ggtcgaagga gtgaatggta cgcaccactt ccagcggttt attcggatcg gcaaccggtg
     8221 tacccaccag cgactgctcg taaggaccga cgtcatcatt gaagttacgc ggaccagagt
     8281 tccaggttga tggaacaacc gcctggtagt tgctgatgat accgtcttta ataaccatcc
     8341 agtgagagag cataccgcgc ggcgcttcga ggaagccaac acctttgaac tcacccgttg
     8401 ccggaatgtt cggcttcaca aaggtggtgt gatcgccttt gccgatattg gtgatcagtg
     8461 cactgtattg gttttgcagg atatcctgca attcacagca gtgaacggta cgaccaataa
     8521 tacggcccag cgtggagtgc agctgtgcca cttccagcgt gttgccagtc agtttctgat
     8581 aaatcgcaac gatttcattc agtttgttct gggtagattc gcgacctgcc gccagtttca
     8641 ccagcatatt agccagcgga cccacttcta ccgttttgcc gtagaaagtc ggtgatttca
     8701 cccaggaata tttcccgtcg tcagaccaac catcataagc cggaatggtg gtaccttccc
     8761 acggcgcctg tggcgcttcg tctttatacc aggagtgctt cgcgctttcc tgaatgcctt
     8821 tgatcaggta ttcatcggaa tgagaagtga tcggacgata cgaggacaga tccgcattct
     8881 caatgtagcc tcccgggaac aggaagctgc cgtttttgct gtcggtcggg aattccggca
     8941 cgctcaggta gttcaccgca cctttaccgc gcgtcagcca ttccgggtag aacgcggcga
     9001 taaccgcggt atcaacctta taaacctgct caacaaagtc gctcagtttg tcgatgaaag
     9061 acttgatgta catcaggcgc tcaaggttca acacgcccaa cccgtcgagg ttgattgggt
     9121 tcgcgacacc acccacagcc aggttctgaa tgtgcggcgt tttaccgccc agcagcgcca
     9181 cgacgcggtt agcgtcacgc tggcactcca gcgcctgcag gtagtgcgct accgcaatca
     9241 ggttcacttc cggcggcagt ttcatcgccg ggtgacccca gtagccattg gcgaaaatac
     9301 ccaactgacc gctggcaacc agatctttga tcttgttctg aactttagtg aactcttccg
     9361 gactgttcag atgccaggtc gaaacgcctt tcagcatttc ggaggctttg gttgggtcag
     9421 cttgcagtgc agaagtgatg tccacccagt ccagcgccga aagctgatag aaatgaacga
     9481 tatggtcatg cgtggtgtgc gcagccagaa tgatgttacg gatgtattgc gcgttaaccg
     9541 gaacgtcgat attcagcgca ctttctgccg cacgaacgga agacagcgcg tgagtggtag
     9601 tacatacgcc acagatacgt tgcacaatca tccatgcatc gcgcggatcg cggtttttca
     9661 cgatctcttc catgccgcgc cacatggtac cggaagccca tgctttcgaa acgacgccat
     9721 tttcgatttc gcaatcgatg cgtaaatgcc cctcaatacg ggttaccgga tcaatagtaa
     9781 ttctctggct catgctttgc tcgcctcatg acgattatga tcgttttgtt ttaaaggagg
     9841 aagtatcggc agtagacgaa tgagtacgat gtaagcgcaa atctcaatag ccacaaaacc
     9901 aatagaaatc aacagttctt cccaggtcgg gaagtaggcg taaccgccgc ccgggttgaa
     9961 tgccaccaac gaataggtca gacgccaggt tgcacaacct aacagtgcgc tcagtgctga
    10021 caggaacagc atacgggaat cattacgcag cttcgccaca cgcagaacga ccagcgggaa
    10081 gagcatcagc aggacttcaa tccagaacat cacggagtag aagtcaccgg caaacgctaa
    10141 cgacagcttg tcgcgataga taagctcgcc aaagcgcagc ccgatgaaaa tcgccagcaa
    10201 cacactgatg gtgttggtca gcttgacgaa caggctcttt tcatccggac cgttgccacg
    10261 cagacccgcc tgcaccagtg aaccttcaaa gatgacaatc gagaagccca tgatgaacgc
    10321 cgtcagcagc gagaacagcg gcaacatttc atagctctgc cagagcggat gcaccttgta
    10381 gcccgccgag atcatcagcg accccattga agactggtgc atggtcggca gcagcgcacc
    10441 gagcgcgatg atgaagaaca tcaccttgtt cagacgttgt agcgacacct tccagcccag
    10501 acgttcaaac agtgccggag caaactccag agccatcacg ccgatgtaga tggtcataca
    10561 gaccgccgtc tcgaacagta ccgagttcac gttgaagtga cccggaatgt agaagtacgg
    10621 caggttccag tagcgaccca cgtcgatagt gatcgacaag ccacccagtg agtaaccaaa
    10681 cagactcgcc aacagcgccg gacgcaccag cggatggtat tgcccacggt taaagacgta
    10741 taccgcccac gccagcgccc agccgccaca ggcaaagccg gtgccaatca gcaggtcaaa
    10801 cgcgatccac acgccccacg ggaagccgcc gttcaggtca gagacagagc ccagaccgaa
    10861 caccagacgc ttcacaatca ggagcataca gatgacgatt aacggtccaa aaatcatgac
    10921 cggtttactg atgattttgc cgcccagcgg ttgtggatca tgactcatga tcgtctcctc
    10981 cgtcgtgatg gtcgtttttg gtgttgcgac gaaccagcac ggttaagccc gccagcacag
    11041 ccagtggtag catcatgcct ttatacaggg tgtgttgaat attttcggaa cgcgcaccgg
    11101 tagaaagatc gtccagtttc ggcaggtcga gattttcata aggcacaccc gtcagtacca
    11161 gaacctgagt accgccgccc tctttctcgc cgtacagatg cggataatat ttcggcaccg
    11221 tatgcaggta agtgtcgcca gatttcagcg tctgacgcgg atagtggtat tcgctgccag
    11281 gcttcagcgc cagacgtttt ttcgcctccg ccatcagctc ttcacgcgta ccgaaaatca
    11341 ccgcgcccgc cgggcacact tctacgcagc caggcagacc gcctttatcg agacgttcca
    11401 cacctttctg gttgcacagc tcgcacttat gcagcgcacc aaacgggttg ttgtagtcgt
    11461 acttcggcac gttgtacgga caggcgacca tgcagtaacg gcaaccggtg cacacgtctt
    11521 tgtcgtaatg gacaatgccg gttttcggat cttttttcag tgcagagacc gggcacacag
    11581 agacacagtt cggatcgacg cagtgcatac actgtttctt aatgtacgcg tagccgttct
    11641 cctcctggtc tttgttgacc cctgtgccgc tggtccacac ctgaatgatg ttattggtat
    11701 acggcgacag tttgtcgttg ttcgaccagg tctgttcccc ttgcgggtta cgttcaggga
    11761 agttgatatc ctgacacttg gtgacgcaag cctggcagcc tacgcacaag gtcgagtcgt
    11821 acaacatccc cagcgatccc ggaattggcg ggcggttttc agcagccgca tgactgacag
    11881 acggcagcgc gcccgtcagc aatgccccgc aggaggctgc tttaataaaa ttacgtctgt
    11941 tcacggttat tctccccgtg agtcagcgtt atctttcttt tgctgacgac ccagttcacg
    12001 caccgccatc acgctgacac cggcaaccag cccaaccaca ccgccgagca aaccaatagc
    12061 gcctgcagag acgttgccgc cctctttagc gttaacatcc ggtttctgtg aacgcggagt
    12121 ctgattttcg acgttggcaa gctgatggat gcctttatgg aagccgatac ctttttcgtt
    12181 acagccatag cacgggtgac caatcgccac cggccacaca ccgccaacat cgcagaattg
    12241 cagcgttgag cagttgccgt aagtttctgg ccctttacag ccgaggtggt acaagcacca
    12301 gccttcgcgg tggccttcat caccgaactc tttggcaaaa cgaccagcat cgaagtgcgg
    12361 gcgacgttcg cagtgttcgt gaatcagacg gccataggcg aaggtcggac ggtttttgtc
    12421 atccagtttc ggcggtttgc cgtaagtgat gatgtgcgca acggtcgcga ggaagttgtg
    12481 cgggttcggc gggcagcccg gaatattgat aacggttttg cctggcagaa cttcttgcaa
    12541 gctgactgcg ccagttgggt taactccagc tgcggcaaca ccgccccacg cggagcagga
    12601 accaatagcg ataatggctg ctgagccttc cgccgctttg cggatgtgat ccacaatcgg
    12661 ctcaccggca accatgcaat aaataccgtt atcttttaat gggatggaac catccaccac
    12721 taacacatac tgccctttgt acttctcgag agcgttatgt ttgttctctt cgacctgatg
    12781 accgaaggcg gcggaaagca cttcgtgata ttccagagag atagtctcca gcacgaggtt
    12841 ttctaccgtt ggatgcgttg cacgaagcag agattccgta caaccggtgc actcctgcgc
    12901 gccaatccag ataactggcg gacgctgcgg gttagtaacc gattcggcca tctctgcagc
    12961 ggctttgcta cttaacccca tggtggcggc taatgctgca caaagcttca tgaaatcacg
    13021 acggttaatg ccgtgagaat ggatgagggt gttatctcca gtcatttata gttattccgt
    13081 tgcgagaacc tggcatatat tttgcctcaa tcgcaaaatc aataatgcga tcgatgcgcc
    13141 atttaccaca catttattat ggttatcgat atcatgatac tgcggcgggg cgctaaaacg
    13201 aagggaagaa gcattagtgt aattaattaa agcaagataa tacgtatgtt tgatcaattt
    13261 tcgaggcgat aaccggccat aaaaaagcgc aacgtagaac aggaattatt ctcacgaaag
    13321 aaatccatta ccatctcttt atccagtcca tagcggcgcg tcagaatacc cgcttcccag
    13381 gcgctaagct ggcgatctcc ggtcttttcg tacatcctgt gtgagtagcc taatacaaaa
    13441 ccgcgtttat aatcggcaca gaattttgtc acattaaccg cactgtcggc atgcgttgct
    13501 tttaatcccg ccattaagcc tttaccaaaa tggttattca tcgttcacct cattcattat
    13561 atatcgccat atataacgat attttaggca aagaacgact tttgcgcacg ctattttttc
    13621 atacttgttt aagacgtaat cccgtaagag ataaacctat caggagagat atttatcgaa
    13681 ccatttcagt gtcctttgcc aggcaagatc ggcggcagat ttgtcataac ggggcgtgga
    13741 atcattatgg aatccgtgat taacccccgg atagatataa gcctcataaa ccttattatt
    13801 gtctttcaac gccgcctcgt aaggaggcca gccctcgttg attcgggtgt ccagttccgc
    13861 gaagtggagt agtaaaggcg cttcaatctt cgccacatcg gcagtgggtg cctgacgacc
    13921 ataaaacggc accgcgcagg ccagttccgg atacgcgaca gccgccgcgt tcgatacgcc
    13981 accgccatag caaaatccgg taatacccac tttacctgtc gcttgcggat agcgttgcat
    14041 aaactcaatt gcggcaaaga aatcattcat cagtttggtt ggatcaacct gctgttgcag
    14101 ctcacgacct ttatcatcat ttcccggata acctccaacg gaacttaatc cgtcaggtgc
    14161 cagggcgata taccccgcct tcgccactcg ccgtgccaca tcttcgatat acggattcag
    14221 tccacgattc tcatgcacca cccccacggc tggcgttttg ccgctcattt ttgcgggttt
    14281 caccagataa ccccgtacct cgccgtgacc atttggcgaa ggatacgtga tgtactcagc
    14341 aacaatctcc gggtcggtaa actctacctg agtcgccagc gcataatttg gcttgagcaa
    14401 atcaaacaac gctaatgccg tcatcccgcc caccgcatac ttcgccgcaa ggttgaggaa
    14461 ctcacgtttc gaaattttcc cgtgaacgta atagtcgtag taatccaaca actcttgtgg
    14521 gaaatctttg gcggttaaac gcggcatcgt tgcattcctc aactggtgtt tttttaagca
    14581 aagcataagc gcgtgttttt gcccagtttt tcgtcactct gtgagccaga ctacgggata
    14641 cgcgctggcg aatcgctaaa ctagaaacat tgtttcgaaa ttgaacggtg gaaaggagag
    14701 gtcatggtct ggttagcgaa tcccgaacgt tacgggcaga tgcagtaccg ctattgcgga
    14761 aaaagtggtt tacgcctgcc cgcattatcg ctcggtttat ggcacaattt cggtcacgtt
    14821 aacgcgctgg aatcacagcg tgcaatcctg cgtaaagcgt ttgatttagg cattacgcac
    14881 tttgatttag ccaacaatta cgggccgcct ccaggaagcg cagaagagaa ctttggtcgc
    14941 ctgctgcgcg aggattttgc tgcttatcgc gatgaactga ttatctctac caaggctggc
    15001 tacgatatgt ggcccggccc gtacggctct ggcggttcac gtaaatacct gctcgccagc
    15061 ctcgaccaaa gcctgaagcg tatggggctt gagtatgtcg atatctttta ctctcatcgc
    15121 gtcgatgaaa atacgccgat ggaagaaacc gcctctgcgc tggctcatgc ggtacaaagc
    15181 ggtaaggcgc tgtatgtcgg gatctcctct tactcgccag agcggacgca aaaaatggtc
    15241 gagttgctgc acgagtggaa aattccgctt ttaattcatc aaccttcgta caatttactg
    15301 aaccgctggg tggataaaag cggcctgctg gataccctgc aaaataacgg cgtgggctgt
    15361 attgccttta ctcctctggc tcagggattg ctgaccggaa aatatctcaa cggcattccg
    15421 caagattcac ggatgcatcg tgaagggaat aaagttcgtg gtctgacgcc gaaaatgctt
    15481 accgaagcca acctcaacag cctgcgctta ttgaatgaaa tggcacagca gcgtggacaa
    15541 tcaatggcgc aaatggcgtt aagctggttg ctgaaagatg atcgcgtgac gtcggtattg
    15601 attggtgcca gccgcgcgga gcaactagag gagaacgtgc aggcgctgaa taatctgaca
    15661 tttagcaccg aggagctggc gcagattgat cagcatatcg ccgatggcga gctgaatctg
    15721 tggcaggcgt cgtccgataa atgacctgtt aataacgggc gcgcgaaccg cgcccgaatc
    15781 agatcagtga ttatgacgag tcagtcggtc aagatagccc atcacaaatg cagaaagcac
    15841 aaacgtcaga tggataatga cgtaccacat cagtttgtta tccgggacat ttttcgcatc
    15901 cataaagacg cgcagtaagt gaatggaaga aattgccaca atcgacgctg ctactttgtt
    15961 tttcagcgac gttgcgtcca ttttccccag ccacttcagc ttctctttgt tctcggagat
    16021 atccagctgt gagacgaaat tctcataacc ggaaaacatc accatcacca gcaaaccgcc
    16081 aaccagcgtc atatccacca gcgacagcaa cacgaggatc aaatctgatt ccgccatcga
    16141 gaagatattc ggcagtacgt gaataatctc ctggaagaac ttcagcgcca gggcaactaa
    16201 cgccagcgaa aggccaaagt acacgggggc aagcagccag cgagaagcat acattgcatt
    16261 ttcaagaaaa cgttccatac attcctgtcg tgtagttaaa acgggcaaca gtatatcgca
    16321 attgagtaaa cagcacgcaa cagcaaatca acatatacgt taaacctttg tcgcataatt
    16381 ctgaggttta gggatcttcc tggttccctt atcaacgcat cgagagcgtc tacccttaaa
    16441 ggacgttgaa cctgaaggga gaaaaacgat gtctcattta aaagacccga ccacgcagta
    16501 ttacactggt gaatatccca aacagaaaca accgacgcca ggcatccagg cgaagatgac
    16561 accgataccg gattgcggcg agaaaaccta tgttggtagc ggtcgcctga aagatcgtaa
    16621 agcactggtg acagggggcg attccggaat aggtcgcgct gccgccatcg cttacgcgcg
    16681 tgaaggggct gacgtggcga tcagttatct tcccgtggaa gaagaagacg ctcaggatgt
    16741 gaaaaagatc attgaagaat gcggacgcaa agccgttctg ctgccaggcg atttaagcga
    16801 tgagaaattc gcccgttcgc tggttcacga agcgcacaag gcgttaggcg ggctggatat
    16861 tatggcgctg gtcgccggga aacaggttgc cattccggat attgcagacc tcaccagcga
    16921 acagtttcaa aagacctttg ccattaacgt tttcgcgctg ttctggctaa cccaggaagc
    16981 gatccccctg ctaccgaaag gtgcaagtgt catcaccact tcgtcaatcc aggcatacca
    17041 gccaagtccg catttactgg actatgcggc tacgaaggcg gcgattctga actacagccg
    17101 tggcctggca aaacaggtcg cggagaaagg tattcgggtg aatattgtcg cgccaggccc
    17161 tatctggacg gcactgcaaa tttccggcgg acaaacgcag gataagatcc cgcagtttgg
    17221 tcagaaaacg ccgatgaagc gtgcggggca accggcggaa ctggcccctg tatatgttta
    17281 tctggcaagt caggagtcga gctacgtcac cgcagaagtg cacggcgtgt gcggcggcga
    17341 gcatttaggt taaaaaaatg cccggttgtg aaaagcaacc gggcatcatt gtgaattcac
    17401 tcttacctga tgtggcgaaa acgccttatc cgcctacaaa cgcatgaaga ttcaacatgt
    17461 tgcaatagat tttgtaggcc tgataagcgt agcgcatcag gcattgagca cctaatgccg
    17521 gatgcgtcgt gaacgcctta tccggcctac aaacgcacga agattcaaca tgttgcaata
    17581 gattttgtag gcctgatgag cgtagcgcat caggcaattc tactttactt cgctttggcg
    17641 gtttcttcgc cgaccagacc tatcttcagg taacccgcct gatgcagcgt atccattacc
    17701 ttcatcaacg tctcgtaatc gacggttttg tccgctcgga agaagatggt ggtgtctttc
    17761 ttgccttcgg ttaacgcatt caacgccgta atcattgttt catcggtgac cggatcgtta
    17821 ccgataaaca tcgagttgtc tgccttcacc gacagataaa ccggtttttc cgggcgcggc
    17881 tgcggcgtgc tggtagaagc aggcaagttc accttcacat ctaccgtcgc taacggtgcc
    17941 gccaccataa agataatcag cagaaccaac atcacgtcga taaacggcgt cacgttgata
    18001 tcatgcattt cgccgttatc gtcgaggttt tcgttaagat gcattgccat cggacattat
    18061 cctgcgcgta atttttgtgc gacacgaacc ggatgcgcag cggcgctggc ttccagatcc
    18121 aggtcacggc tttgcagcaa caatacctgc gctgcaacat cacccagcat cgctttaaag
    18181 ccgccaatct ggcgtgcaaa tacgttatag ataacgaccg caggaatcgc tgccacgagg
    18241 ccgattgccg ttgctaacag agcttctgcg atacccggcg caacgactgc caggttagtg
    18301 gtctgcgttt gcgcgatacc aataaagctg ttcatgatgc cccagaccgt accaaacaga
    18361 ccaacgaacg gagaaatcgc gccgatggtt gccagatagc cgttaccgcg gcccatttga
    18421 cgacccactg cggcgacccg acgctccaga cggaagctgg tacgttcttt aataccttcg
    18481 ttatcgtcgc tgccttctga cagttccagc tcattctgcg cttcattgag caaatgcagg
    18541 cttaagcttt tgctaccaaa atcagcggcg atatcgttgg cctggtttaa ggaacgtgct
    18601 tcagccagca gttgctgctc gcgcttaagg cgacgcttct gattgaagaa ctctacgctc
    18661 ttactgaaga agattgccca ggtgactaca gaggccaaaa taagcccaat catcacgcac
    18721 ttaacgacaa tatcggcgta ctgatacata ccccagacgg aaaggtccgt ctgcattaaa
    18781 ttattaccca ctgtgtatct ccaggacgca agtcacaaaa tctgcgcata ataatatcaa
    18841 aacgacgtcg aattgatagt cgttctcatt actatttgca tactgccgta cctttgcttt
    18901 cttttccttg cgtttacgca gtaaaaaagt caccagcacg ccatttgcga aaattttctg
    18961 ctttatgcca attcttcagg atgcgcccgc gaatattcat gctagtttag acatccagac
    19021 gtataaaaac aggaatcccg acatggcgga caaaaagctt gatactcaac tggtgaatgc
    19081 aggacgcagc aaaaaataca ctcttggcgc ggtaaatagc gtgattcagc gcgcttcttc
    19141 gctggtattt gagagtatgg aagccaaaaa gcacgcgacg cgcaatcgcg ccaatggcga
    19201 gttgttctat ggacggcgcg gaacgttaac ccatttttcc ttacaacaag cgatgtgtga
    19261 actggaaggt ggcgcaggct gcgcgctatt tccctgcggg gcggcagcgg ttgctaattc
    19321 gattcttgct tttgtcgaac agggcgatca tgtgttgatg accaacaccg cctatgaacc
    19381 gagtcaggat ttctgtagca aaatcctcag caaactgggc gtaacgacat cgtggtttga
    19441 tccgctgatt ggtgccgata tcgttaagca tctgcagcca aacactaaaa tcgtgtttct
    19501 ggaatcgccg ggttccatca ctatggaagt ccacgacgtt ccggcgattg ttgccgccgt
    19561 gcgcagtgtg gtgccggatg ccatcattat gatcgacaac acctgggcag ccggtgtgct
    19621 gtttaaggcg ctggattttg gcatcgatgt ttctattcag gccgccacca aatatctggt
    19681 tgggcattca gatgcgatga ttggcactgc cgtgtgcaat gcccgttgct gggagcagct
    19741 acgggaaaat gcctatctga tgggccagat ggtcgatgcc gataccgcct atataaccag
    19801 ccgtggcctg cgcacattag gtgtgcgttt gcgtcaacat catgaaagca gtctgaaagt
    19861 ggccgaatgg ctggcagaac atccgcaagt tgcgcgagtt aaccaccctg ccctgcctgg
    19921 cagtaaagga cacgaattct ggaaacgaga ctttacaggc agcagcgggc tattttcctt
    19981 tgtgcttaag aaaaaactca atgatgaaga gctggcgaac tatctggata acttcagttt
    20041 attcagcatg gcctactcgt ggggcgggta tgaatcgttg atcctggcaa atcaaccaga
    20101 acatatcgcc gccattcgcc cacaaggcga gatcgatttt agcgggacct tgattcgcct
    20161 gcatattggt ctggaagatg tcgacgatct gattgccgat ctggacgccg gttttgcacg
    20221 gattgtataa cattgccact tttggacaat tttgcagaca ttttattgtg aaaagtctta
    20281 aattgttgcg tccgggatca aggcgtcccg gacgattcag gagtacaata ggcagataaa
    20341 ggcttaaacg ctgttccaca ggaaagtcca tggctgttat tcaagatatc atcgctgcgc
    20401 tctggcaaca cgactttgcc gcgctggcgg atcctcatat tgttagcgtt gtttactttg
    20461 tcatgtttgc cacgctgttt ttagaaaacg gcctgctgcc cgcctcattt ttgccaggcg
    20521 acagcttgtt gatactggca ggcgcattga ttgcccaagg ggttatggat tttctgccta
    20581 cgattgcgat tctgaccgcc gcagcaagtc tgggctgctg gctaagttat attcagggga
    20641 gctggttagg gaataccaaa acggtgaaag gctggctggc acagcttcct gctaaatatc
    20701 accagcgcgc cacctgcatg tttgaccgcc acggtctgct ggcgctgctg gctggacgtt
    20761 ttcttgcatt tgtccgtacg ctgctgccaa ccatggcggg aatttccggt ctgccaaacc
    20821 gccgcttcca gtttttcaac tggttaagtg gattgctgtg ggtcagcgtg gtaaccagtt
    20881 ttggctatgc cttaagtatg attccgttcg ttaaacgcca tgaagatcag gtaatgacgt
    20941 tcctgatgat cctgccaatt gccttgttaa ccgctggctt gttaggcacg ctgtttgtgg
    21001 tgattaaaaa aaaatactgt aacgcctgac gattttcccc gttcccggct gctgtaccgg
    21061 gaacgtattt aattcccctg catcgcccgc attcttgccg catcttcccc cggcgtcaca
    21121 ccgaagtaac gtttaaactc acggctaaat tgcgatgcgc tttcatagcc gacgcgcatc
    21181 gctgctgcgc tggccttcat gccgtcatgg atgatcatca tccgcgcctt atgcagacgg
    21241 taattcttca aatactgcaa cggcgaggtg ctggtgacag acttaaaatt atggtggaac
    21301 gccgatacgc tcatgttggc ttctgccgcc agttgctcga cgctcaggtt ttcggtgtat
    21361 ttattctcaa ttcgtttcag cacgcggcta atcagactga agtgagtctg gcgactgacc
    21421 agcgccagta acgcgccgcc gcaaggtccg gtcagcacgt agtacagaat ttcgcggatg
    21481 atctgtttgc cgagaatacg cgcatccagt ggtcgctcca tcacgtcgag taaccgctcc
    21541 gccgcgcata aaatctcttc tgataacgtg gcggagttaa tcccgctggc tgccatcgac
    21601 ggctggaaat gctcatcttc gccaatgtcc atcaacagtt cctgtaactg caaaatatcg
    21661 acattgagac gcaaccctgc cagcggcacc tctgacgtgg cataggtttc gcactcaaac
    21721 ggcaacggca ccgtcagcag caggtattca ttggcatcat aacgaaacac gcgttcattg
    21781 atataaccga ttttatgccc ggaaaagaga attatgatgc caggctcgta catcaccggt
    21841 gtacgtgcga aaggcgtctc gccatacaac aaacgcacat cgggcaacag ttctgacaaa
    21901 ctattttctt tatttttcag tttattaact ttatccgcca gcaagcgaca aatctcttca
    21961 cgtttcatat cgcgtaattt cttaggaata atgcggcaat ttgattgtgc gcaattttgt
    22021 agcatttctc cagcactctg gagaaatagg caagacattg gcagaaatga gcattgagag
    22081 ccagggcgct ggcgatcaca atgaaaaaca tcaggcagat cgttctctgc cctcatattg
    22141 gcccagcaaa gggagcaagt aatgaacaac tttaatctgc acaccccaac ccgcattctg
    22201 tttggtaaag gcgcaatcgc tggtttacgc gaacaaattc ctcacgatgc tcgcgtattg
    22261 attacctacg gtggcggcag cgtgaaaaaa accggcgttc tcgatcaagt tctggatgcc
    22321 ctgaaaggca tggacgtgct ggaatttggc ggtattgagc caaacccggc ttatgaaacg
    22381 ctgatgaacg ccgtgaaact ggttcgcgaa cagaaagtga ctttcctgct ggcggttggc
    22441 ggcggttctg tactggacgg caccaaattt atcgccgcag cggctaacta tccggaaaat
    22501 atcgatccgt ggcacattct gcaaacgggc ggtaaagaga ttaaaagcgc catcccgatg
    22561 ggctgtgtgc tgacgctgcc agcaaccggt tcagaatcca acgcaggcgc ggtgatctcc
    22621 cgtaaaacca caggcgacaa gcaggcgttc cattctgcac atgttcagcc ggtatttgcc
    22681 gtgctcgatc cggtttatac ctacaccctg ccgccgcgtc aggtggctaa cggcgtagtg
    22741 gacgcctttg tacacaccgt ggaacagtat gttaccaaac cggttgatgc caaaattcag
    22801 gaccgtttcg cagaaggcat tttgctgacg ctaatcgaag atggtccgaa agccctgaaa
    22861 gagccagaaa actacgatgt gcgcgccaac gtcatgtggg cggcgactca ggcgctgaac
    22921 ggtttgattg gcactggcgt accgcaggac tgggcaacgc atatgctggg ccacgaactg
    22981 actgcgatgc acggtctgga tcacgcgcaa acactggcta tcgtcctgcc tgcactgtgg
    23041 aatgaaaaac gcgagaccaa gcgcgctaag ctgctgcaat atgctgaacg cgtctggaac
    23101 atcactgaag gttccgacga tgagcgtatt gacgccgcga ttgccgcaac ccgcaatttc
    23161 tttgagcaat taggcgtgcc gacccacctc tccgactacg gtctggacgg cagctccatc
    23221 ccggctttgc tgaaaaaact ggaagagcac ggcatgaccc aactgggcga aaatcatgac
    23281 attacgttgg atgtcagccg ccgtatatac gaagccgccc gctaagcttt ttacgcctca
    23341 aactttcgtt ttcgggcatt tcgtccagac ttaagttcac aacacctcac cggagcctgc
    23401 tccggtgagt tcatataaag gaggaacgta tggctaatcc aaccgttatt aagctacagg
    23461 atggcaatgt catgccccag ctgggactgg gcgtctggca agcaagtaat gaggaagtaa
    23521 tcaccgccat tcaaaaagcg ttagaagtgg gttatcgctc gattgatacc gccgcggcct
    23581 acaagaacga agaaggtgtc ggcaaagccc tgaaaaatgc ctcagtcaac agagaagaac
    23641 tgttcatcac cactaagctg tggaacgacg accacaagcg cccccgcgaa gccctgctcg
    23701 acagcctgaa aaaactccag cttgattata tcgacctcta cttaatgcac tggcccgttc
    23761 ccgctatcga ccattatgtc gaagcatgga aaggcatgat cgaattgcaa aaagagggat
    23821 taatcaaaag catcggcgtg tgcaacttcc agatccatca cctgcaacgc ctgattgatg
    23881 aaactggcgt gacgcctgtg ataaaccaga tcgaacttca tccgctgatg caacaacgcc
    23941 agctacacgc ttggaacgcg acacacaaaa tccagaccga atcctggagc ccattagcgc
    24001 aaggagggaa aggcgttttc gatcagaaag tcattcgcga tctggcagat aaatacggca
    24061 aaaccccggc gcagattgtt atccgctggc atctggatag cggcctggtg gtgatcccga
    24121 aatcggtcac accttcacgt attgccgaaa actttgatgt ctgggatttc cgtctcgaca
    24181 aagacgaact cggcaaaatt gcaaaactcg atcagggcaa gcgtctcggt cccgatcctg
    24241 accagttcgg cggctaacgt gcaaattctc ccggtggcgg taatgttccg ctaccggact
    24301 tttcagaaat catttattcc cctcgcgtcc cgcccgttgt tactcttcct tgttcaggaa
    24361 tgccaaatat aaggacatca tcatgcagag ccggaagctc ttaaaagaac aactcatcta
    24421 tatccgggat aaacgcaacg gagaggtgaa aaacagatga aaataatact tctgttttta
    24481 gcagccctgg caagttttac cgtacacgcg cagcctcctt cacagaccgt agaacaaaca
    24541 gtccggcaga tttatcagaa ctataaatca gatgccagta cgccttattt cggtgaaacc
    24601 ggagagcggg cgataacttc agcgcgtatt caacaggcgc ttaccctgaa cgacaatctt
    24661 acactaccgg gcaatattgg ctggctggat tatgatccgg tttgtgattg tcaggatttt
    24721 ggcgatctgg tgttagaaag cgtagcgata acccaaactg acgtcgatca tgccgatgcc
    24781 gttgtgcgct ttcgtatctt taaagatgat aaagaaaaga ccacacagac actgaaaatg
    24841 gtggcggaaa atggtcgttg ggtcattgat gatattgtca gcaatcatgg cagcgtctta
    24901 caagcagtta atagcgagaa tgaaaaaacg ctggccgctt tagcttcgtt gcagaaagaa
    24961 cagccagaat cctttgttgc tgaactcttt gaacatattg ctgattatag ctggccgtgg
    25021 acgtgggtgg tttccgactc ttaccgccag gcggttaatg ccttctataa aaccaccttc
    25081 aagacggcca ataatcccga tgaagatatg caaatagaac ggcaatttat ttacgacaat
    25141 ccgatctgtt ttggcgaaga gtcgctattt tcacgcgttg atgaaattcg agtcctggag
    25201 aaaaccgccg attccgcccg cattcatgtt cgttttacgc tgaccaatgg caacaatgaa
    25261 gagcaagagc tggttttaca gcggcgcgaa ggtaagtggg aaatcgctga ttttatccgc
    25321 ccgaacagcg gcagcctact taagcagatt gaggcaaaaa ccgccgccag attaaagcaa
    25381 tgagctgaat taaataacaa ttagccggaa caataaataa aagggaacac tatatgaaaa
    25441 cgattttcac cgtgggagct gttgttctgg caacctgctt gctcagtggc tgcgtcaatg
    25501 agcaaaaggt caatcaactg gcgagcaatg tacaaacatt aaatgccaaa atcgcccggc
    25561 ttgagcagga tatgaaagca ctacgcccac aaatctatgc tgccaaatcc gaagctaaca
    25621 gagccaatac gcgtcttgat gcacaggact attttgattg cctgcgctgc ttgcgtatgt
    25681 acgcagaatg ataaaaaaat ccccggcagc atgtcagttg ccggggattt tttttaacgt
    25741 ccaaccgccg ctttagggcg tttcttcgca ccagcattca ccggacgaga ttgcgtagac
    25801 gacgcttttt ttgccgtagc aggcgtctga cgctgagtcg ccatcggcgt atgtttagtc
    25861 aacgccggac gggtattgcg gttctggcga cgagcttcac gcatctcttc aatggttggc
    25921 gcaggcacta agcaatcgcg acggctgcca atcagatgct ttttgcccat cgcttccagc
    25981 gcctggcgga ttaacggcca gtttgccgga tcgtggtaac gcaacaacgc tttatgcaaa
    26041 cgacgctgtt tgtcgccctt cggtacgaag acgccttcgc tcttataacc aatcttcgcc
    26101 agcggttttt tcccggtgta atacatggtg gttgagttcg ccagcggcga tgggtagaag
    26161 ttctgtacct ggtcgagacg gaaacgatgc tttttcagcc acagcgccaa attcaccata
    26221 tcttcatcac gcgtaccggg gtgcgcagag atgaaatacg ggatcagata ctgctcttta
    26281 cctgcctgtt tcgagtaggt atcgaacagc tctttaaagc ggtcatagct gcccatgccc
    26341 ggcttcatca tcttcgacaa cggcccttct tcggtatgtt ccggagcaat cttcagataa
    26401 ccgccgacgt gatgggtcgc caactctttg atatagcgcg gatcttccac ggcgatgtca
    26461 taacgtacgc ctgaggcgat aaggattttt ttaatgcctt tcagatcacg cgcacggcga
    26521 tagaggttga tcgtcggttc gtggttagtg tccatgtgcg gacaaatatc cggataaacg
    26581 cacgacaaac ggcgacaagt ttgttcagcg cgtggcgatt tgcagcgcaa catatacatg
    26641 ttggcagttg gcccaccgag atcggaaatc acgcccgtaa aacctggaac ggtgtcgcgg
    26701 atcgcttcga tctcattaat gatcgaatct tccgaacggc tctgaataat gcgcccttcg
    26761 tgctcggtaa tagaacagaa agaacagccg ccaaagcagc cgcgcataat gttgaccgaa
    26821 aaacggatca tttcgtaagc cggaatacgg gcattgccat aggccggatg tggcacgcgc
    26881 ttgtacggca gcgcaaaaac gctgtccatc tcttcggtag aaagcgggat agcaggcggg
    26941 ttgatccaca catagcggtc accgtgtttt tgcatcaatg cgcgggcgca gcctgggttg
    27001 gtttcgtggt gcagaatgcg cgaagcatgg gcgtacagca ctttatcgcc cttcactttc
    27061 tcgaaagaag gcagcaatac gtaggttttt tcccacggtt tctggcgcgg tggctgcacg
    27121 attacagctt tcgcttcctg ctttttcggc gcaacaggtt tgttatccgc acacggcaaa
    27181 tcttcgccat acggatgcgg gattgggtcg attttcccta gggtatcaag acgggtggaa
    27241 tccacaccgc tccagccagg cagcgcctct tttacgataa ttgcggtatt acgcacatcg
    27301 cggatttcac taatcggctc gcccatcgcc agacgatgcg ctacttccac cagcggacgt
    27361 tcaccgttac caaacatcag catgtcggct ttcgaatcta ccagaacgga acggcgcacg
    27421 gtatcggacc agtaatcata atgcgcggta cggcgcaggc tagcctcaat accgccgagg
    27481 atcaccggca catctttcca cgcctcttta caacgctggg tataaaccag cgtggcgcga
    27541 tccggtcgct tacccgcgac attatctggc gtgtaggcat catcatgacg taaacggcga
    27601 tcggcggtat agcggttgat catcgaatcc atgttgccag cagtaacacc aaaaaacaga
    27661 ttcggtttac ccagacgcat aaagtcgtct ttgctgctcc agtccggctg ggcgatgatc
    27721 ccgacgcgaa agccctgcgc ttccagcata cgaccgcaaa tcgccatccc gaagcttggg
    27781 tgatcgacat acgcgtcgcc agtaaccaaa atgatgtcgc agctatccca gccaagttga
    27841 tccatctctt cacgagacat cggcaaaaac ggtgccggtc caaaacaggc ggcccagtac
    27901 tgcggccagg agaacaggtc gcgatccggt tggatcaggg agatagagct cattttgctt
    27961 ccagaaatga taaaaaaata atcaaaggcc ggggattata agccggaacg aaagagaaat
    28021 cgaaaggtat tccatactcg ccctcctcgg gcgagtatga agattacggt accggattga
    28081 ccaacagttg cccaatcgag ccacggtccg ccatttccag cgtctgactg ttgaagtaga
    28141 acgggaagtg cgcccaggaa ggctgaccga aataaacaag cagctccact tgtccgtcaa
    28201 cccaaacggt atctttccag cctctgtctt ccgggaacgg catcgcgcca ttcacgttac
    28261 ggatctggaa cattacgcct tcaatatgga acgcttgcgg ctcgtccgcg cgtaccgtcc
    28321 agcgttccca cgttccttgc tgcgcggtga catcaatacg gttgacgtcc cacagctgtc
    28381 cattaatacc cgggtcatca cccagactga tatcgcgact gcgaattggc gaaccagcca
    28441 tgatttcagt tggcagcaag cgcatcggaa gactgtctgt gaccagcggc agaaggccgg
    28501 ttgggcgcag cgttagcacc aaggtagaaa ccagaatact ggatggctca aagaagccgc
    28561 gaatacgatc aacaatgctc gccgcttcac cacaggtgat cgacacttca tcgccgttgc
    28621 tcatatccac cagaatctcg cggcgctcgc ccggtgccag cgaaagttgc ttcaccgata
    28681 caggagcagg caggaatccc tgatcgccag aaatcacatg taacgggcga ccatcgctca
    28741 tctgtagttg atagcgacga gagttcgacg cgttcagcag tcgcaagcgc acccagccac
    28801 gcgagacttc aacgtacggg ctttgtacac cgttaaccag cagcgtatca ccaacaaagc
    28861 cgccgcttcc cggttcgttg tattctggcg taccaaagtt atccagccgt ttatcctgga
    28921 taatgaccgg aaaatcatcc acaccataat ggttggggat aggcagcgac ttgctgactt
    28981 catcttccac cagccacatt ccggcaaggc cgttatagac ctgctgagcc gtgcggttgg
    29041 gagtattggc gtgataccac agagtagctg cgttctggcg aataggcagt acgggtgccc
    29101 agtcagcgtt tggcgacatc atccgtgccg gaccgcccat cagcgggcct ggtacctgta
    29161 gcccggccac cgtcattgag acattttctg tcaggcggtt gctgtaaata agcttaacat
    29221 cgtcgccctt ccagacgcgg atagtcggcc ccaggtaacg accattgatt ccccagaccg
    29281 acgcgcgtgt ccctggcgta aatgaccagt gcgcacgttg tacagtcata aacagcggtt
    29341 gcccacggcg agattcaagt agcggcggaa cgggtagcgg ttgctgttgc ccggctgcgc
    29401 tggccttcag gggaacagcg cctgcacaaa gtgcaatccc cgatgcctga atgaactgac
    29461 gccgactgag tgacatattt gctccatgta aaactggcta aagtaatacc gtgcaggaaa
    29521 gtgttccccg caaaaataca gattgccgac ttaaactttt ccggcggctt cgcgttctgc
    29581 gacttcttta tcgagctcgg cgattttttg ttccattatc gaacgacaat gggcagccag
    29641 ctcacgaacc tgatctttgc catactgact gacgtcaatt ggcggcagca tttcgacaat
    29701 caccagaccg ttgtgcagtc gattaagatt aatcttattc gaagttgtag agacgcacac
    29761 gggaataatc gggacgcccg ccgcaattgc cgcgtgaaat gctccagtct tgaacggtag
    29821 caggccgcga ccacggctgc gggttccttc cgggaacatc cagatggaaa tacggcgttt
    29881 tttgaaatga ttcacgactt ccgcaatggt gccgtgagct ttagtgcgat tgtttctgtc
    29941 gatcaataag ttgccggtta accagtacaa ctgcccgaag aaggggatcc acagcaagct
    30001 ctttttacct accgtcaccg tcggcggttg cacaatgttc gatgctgtca ccatgtcata
    30061 gttgttctgg tggttagcga tatagatagc attgccgtag ctttcagcat ctgcaggttt
    30121 gcggcactca actttcaggc cgaacagtgg cgcaagacgg ccaaacatat gcccaaaggt
    30181 ggccacatgt ttcgggttac gcgggctgaa aaggcagtaa atggagccga atacacagac
    30241 taagatgctg taaatcacgg taataataag acgaaagata tatagcataa caccctctga
    30301 aggtctgaga gactggcatt gtacgtcacc tgaaatcagg ttagggcatt gttaaaagcc
    30361 gctctctgga aaagcgtatt gttaatcgca acgcatgaat aaacaacggt ttcaagggaa
    30421 tttttatcgt ttccgggggt tgaatacgct gccggatgac gacttaacgt ttcatccggc
    30481 gttccttgct agcgggagga aacagcgccc tccccggcat attactcttc gctatcaccg
    30541 ctgctggcac ggcgaggaga gtcgatctca acacgatcga tacgctgcaa accgcgcatc
    30601 aacgtaccgc ggcgtccacg ttcgccagtg actttctgta gctcttccgg acgcagttta
    30661 attttgcgtt tcccaacatg aatggtcagc gtgctttgcg gcggcagaac gtacagttgc
    30721 gccagaccat cctcgccacg cgcggcttct gccgatggaa tgttgataat cttgttgcct
    30781 ttgcccttcg acagctgcgg cagatcgctt accgggaaca tcaacatacg gcctgcctga
    30841 gtgattgcca gcagcatatc ggaagcatct tcaatcacca ccggcggcat aacatgggca
    30901 ttttccggta aggtgatcaa agccttaccc gcacggttac gcgctaccag atcgttaaag
    30961 gtgcagacga aaccgtaacc cgcatcggaa gccatcagca gtttctgatc gtcgctttcc
    31021 atcagcatat ggtcaacggt cgccccaggc ggcaacgtta atttgccggt gagtggttcg
    31081 ccctgaccac gcgccgacgg cagcgtaatc gggtcgatgg catagctacg accggtggaa
    31141 tcaacaaaca ctaccggttg gttactctta cctttcaccg ccgctttgaa gctatcaccc
    31201 gctttataat tcaggcccgg cgcgtcgata tcatggcctt tagcgctgcg tacccagccc
    31261 atctgcgaca gcacaatggt gacaggttca gacggcagca tgtcgtgctc gctcatcgct
    31321 ttcgcttctt cgcgttcctg caacggcgaa cgacgatcgt caccgtaggc ttgcgcgtct
    31381 gcctgcagtt ctttcttcag caggttattc attttacgct cggaagccaa aatgccctgc
    31441 aactggtcgc gctctttttc cagctcactc tgctcaccgc gaatcttcat ctcttccagt
    31501 ttggcaagat gacgcagttt cagttcgagg atcgcttccg cctgggtttc cgtaaggcca
    31561 aaccgcgaca tcagcgccgg tttcggttca tcttcgttac gaatgatctc aatcacttcg
    31621 tcgatgttga gaaacgccac cagcaaacct tccaggatat gcaggcgctt gaggactttc
    31681 tccagacgat agttcagtcg gcggcgcacg gtatcgcgac ggaacaccag ccattcggag
    31741 aggatttcca gcaggttttt caccgccgga cgaccatcca gaccgatcat attgaggtta
    31801 atgcgatagc tcttttccag atcggtggta gcgaagaggt ggttcatcac ctgatccata
    31861 tccacgcggt tggaacgcgg cacaatcacc agacgggtcg ggttctcgtg gtcagattca
    31921 tcgcgcagat cgtcaaccat cggcagcttt ttgttgcgca tttgcgcagc aatttgctcc
    31981 agtacgcgcg cacctgaaac ctgatgcggt aatgcgctga taaccaccgc gccatcttct
    32041 ttcttccaca ccgcgcgcat acgcactgaa ccacgtccgt tctcgtagat tttacgaatc
    32101 tcggcgcgcg aagtgataat ttccgcttca gtcggataat ccggcccctg cacgatatcc
    32161 agcagctgat cgagcgtagt tttcggctgg tcgattaatg cgattgccgc ctgagctact
    32221 tcacgcaggt tatgcggtgg aatatcggtc gacatgccga cggcaatacc ggtggtgccg
    32281 ttaagcaaaa tgtttggcag acgggcaggt agcattttcg gctcctgcaa agtgccgtcg
    32341 aagtttggca cccagtcagc cgtcccctgc cccagctcgc tcaatagcag ctcggaatat
    32401 ttcgacaacc gggattcggt gtaacgcatt gccgcgaacg atttcggatc gtccggcgcg
    32461 ccccagttcc cctgaccatc aaccagcgga taacggtaag agaacggctg cgccatcagg
    32521 accatcgctt cataacaggc gctatcgccg tgcggatggt atttacccag tacgtcaccg
    32581 acggtacggg ccgatttttt aaatttggcg ctggcattca ggcccagttc agacatcgca
    32641 tacacaatgc ggcgctgaac aggtttcaga ccatcaccaa taaacggcaa cgcacggtcc
    32701 atgatcacgt acatggagta gtttaagtag gcgttttccg taaattcatg tagcgcaagg
    32761 cgctctgcca tatcgctcat taattctgat tcctcaactt attcgccagc cttcaaaggg
    32821 gcaatattgc cggagatact acattatctg ccgcgtcgag tcacaggaga tcagcgtgaa
    32881 tagcgccgcc ggacgtgctc cgacggcgat aaaggcatca atgtgccttg atatacaact
    32941 ctttgctgta gacttgatcg gcatagaggg cagcacaagg ttgcgcccat gccacaccac
    33001 tcggcatggg ggtccgctcc gggcgattcg agaagttgag gttatgcgag tccagggaag
    33061 cctaaagcat caaagtagtc gactgtaagt atacggttca gcccagttta gctgtgtctc
    33121 caccagtgcc ccgcgcctcc tgccataagt gcggcttgct catggatatc cccgagacgg
    33181 cttacggcgc accggataat aaatttcgcg tatacatggt attccttacc ggagaaatta
    33241 taagcccaac aacaatacgc ctgtgacatt gttcgctcaa acttatcgcg agtgatttgg
    33301 ctcacactcc ggtaaagatt ccccatcacg ccaacggcac ataaacatcc gtttccagtc
    33361 gctgctctgt caccccttcg gcaagattgg tgtagtgaaa cagaatcggt gctttacgca
    33421 ttttctcgcc gcttgcaggc agccagtggc gaataatgcc ccatatcgtg tgtgaaatat
    33481 cgtccagctc gccaacgtgg cgggccacgg cataacgtcc accggtaagt tcaccattgc
    33541 tcacaccata acgattatcg ggaatcggtt cgctaacgct gccgcagata tcaaagcgaa
    33601 acgcttccgg tgcggtggtg gcaggggcgt cccaggcgac gccaaaagtc tggctttggt
    33661 taacaggaga aagccccgtc tctttacgcc acatgataaa tttcgccaca ctgtagttga
    33721 gcaactcagg gctacagcgg tgcggcaaca tcgccacagg gattgatggg aaatcgataa
    33781 tgtttacatc cagtgtcagg tttgtcattt tgcctccctg cgaaattatt cccgggaacg
    33841 cgtcccggga gcggtaacaa ttacggattt actttgcgga tctgtttgac gtcaatttca
    33901 acagaattcc agtctttatc gacttcaccc tgaatctcaa ccgtatcttt cggcgtcacc
    33961 gtcacaccgt tccagcgttt gtgatcgata tcaacattga tagtaccgct ggcatcttta
    34021 aacacgtaga gatcgtcaga gatgcgttca acgatattgc cgcgcagggt tacccaggtg
    34081 tcgtcacgca gggattttgc gctttctaca gtcgttacgc tgccgttcgg cccctgaaat
    34141 cctccggcct gactttgcgt taccgatggg ccagaaaaac cgccctgctc tgctgccatc
    34201 accggtgcac tgcacagggc cattactgcg attactgctg cgaatttttt catgtttatt
    34261 actcccttta atgtctgttt ccgagcattt aacaagatag tccttaacaa cttcttaagg
    34321 gaaaaaaata aaatttaatg ctgtacagag cgcgttacaa cacggtttac tggcagcaaa
    34381 tacggttatc gcagggatga aaaaatgcga attttactga tagaagatga catgctgatt
    34441 ggtgacggca tcaaaactgg ccttagtaaa atgggtttta gtgtcgactg gtttacacaa
    34501 ggtcgtcagg gaaaagaggc gctttatagc gcaccttatg atgcggtgat cctggattta
    34561 accttaccag gcatggatgg tcgcgatatt ttgcgcgaat ggcgagagaa aggtcagcgt
    34621 gagccggtac tgaacctgac cgcgcgtgat gcgttagcgg aacgtgtaga agggctgcgt
    34681 ctgggagctg acgattatct gtgtaaacct tttgcgttga tagaagtcgc cgccaggctg
    34741 gaggctctga tgcgccgaac caacggccag gccagcaacg agctgcgcca cggtaacgtc
    34801 atgctcgacc ccggcaaacg tatcgccacg ctggctggcg aacccttaac actgaaacca
    34861 aaagaatttg ccctgctgga attactgatg cgtaacgctg gtcgggtact gccgcgcaaa
    34921 ctgattgaag agaaactgta tacctgggac gaagaggtca ccagtaatgc cgttgaagtg
    34981 catgtgcatc atctgcgacg caaactcggt agtgatttta ttcgtaccgt gcatggtatt
    35041 ggctacacat taggtgagaa atgaaattta cccaacgtct tagtctgcgc gtcaggctga
    35101 cgctaatctt tttaattctg gcctcggtga cctggctgct ttccagcttt gtcgcctgga
    35161 aacaaacaac ggataacgtc gatgaattgt tcgacaccca actgatgctg tttgccaaac
    35221 ggttaagtac gctcgatctc aacgaaatca acgcggcgga tcgcatggca cagacgccaa
    35281 ataaattaaa acacggtcat gttgatgacg atgcgctgac ctttgccatc tttacccacg
    35341 acggcagaat ggtccttaat gatggcgata acggagaaga tattccctat agctatcaac
    35401 gggaaggttt tgctgacggg caactggtcg gtgaagacga tccttggcgt tttgtctgga
    35461 tgacctcacc tgatggcaaa tatcgcatcg ttgttggcca ggaatgggaa taccgtgaag
    35521 acatggcgct ggcgattgtt gccgggcaat tgatcccgtg gctggtcgca ctgccgatta
    35581 tgttaatcat catgatggta ctactgggtc gtgaactcgc gccgctgaac aaactggcgc
    35641 tggcactacg tatgcgtgac cctgactcgg aaaaaccact aaacgcgact ggcgtaccca
    35701 gcgaagtgcg gccactggtt gagtcgctaa atcaactgtt cgcccgcaca catgcgatga
    35761 tggttcgaga acgacgcttt acctccgatg cagctcacga actacgtagc ccgttaacag
    35821 cgctgaaagt gcaaaccgaa gttgcgcagc tctctgacga tgatccgcag gcgcggaaaa
    35881 aagcactgct ccaattacat tccgggatcg atcgcgctac tcgtctggtt gatcaactgc
    35941 tcacgctatc gcggctggac tcactggata accttcagga cgtcgcggag atcccgcttg
    36001 aagatctcct acaatcgtcg gtgatggata tttaccacac ggcgcagcag gcgaaaattg
    36061 acgtgcgact gacactcaat gcccacggca tcaaacgcac cggacaaccg ctattgctaa
    36121 gtttgttggt gcgaaatttg ctggataacg gccccggtgt gacaccagag gcactggcgc
    36181 gaattggcga acgcttctat cgcccacccg gacaaaccgc taccggcagt ggacttgggc
    36241 tatcgattgt ccagcgaatc gccaagctgc atgacatgaa tgttgagttt gggaatgcgg
    36301 aacaaggtgg atttgaggcg aaggtaagct ggtaacttct ctgtctatga gagccgttaa
    36361 aacgactctc atagatttta ctaatagcaa aatataaacc gtccccaaaa aagccaccaa
    36421 ccacaaacca gacaaataac gagcgagaat acatatagcc aaaactcgaa catttcccgc
    36481 ataaagagtt ttcttaagat aagaataata agtggcgtaa gaagaaaaaa tgctgcaatc
    36541 attgcttgca atatacgatc ttcgcctgaa acggaaggta tactgcaaat attgcctccc
    36601 gactcaagca tccagtcata cttattgaaa gcaaaaaaag aatctattaa ccaataagta
    36661 agcattatat atagcaatgc ttcaaatatt gttttaattt tctgctttaa catatatcag
    36721 tacgctcata aaaatccatt tataattatc aacatgacac gttgatattt tcatctttta
    36781 cttattcaaa gccatatttt caatccccaa aaaaaccgaa ccaaacgtgt aaattattcc
    36841 ggtgcgagta agaattatga ggaatggcta tcagtattgt cattttcaga aaatatttat
    36901 cctgcatcgg tgagtcagag ctagatcaga cttttgctaa attcgcaaaa gactttgcac
    36961 attttgctaa tttcacagta ccgctctgtg acgtactata gtcgacaaac gtctcacctt
    37021 gagattaaaa aatgagcaac atcctgatta tcaacggcgc gaaaaaattc gcccactcca
    37081 atggtcaact gaacgacacc ctgaccgaag tcgcggatgg cacactgcgc gacctcgggc
    37141 atgatgtccg catcgttcgc gccgacagcg actacaatgt caaagcggaa gtacaaaact
    37201 ttctctgggc tgatgtggtg atctggcaga tgccaggctg gtggatgggc gcgccgtgga
    37261 cagtgaaaaa atatattgat gatgtattca ccgaaggtca cgggactctg tatgccagcg
    37321 atggtcgtac ccgcaaagat ccgtcgaaaa aatacggttc cggcagcctg gtacaggaca
    37381 aaaaatatat gctttctcta acctggaacg caccaatgga agccttcacc gaaaaagatc
    37441 agttcttcca cggcgttggc gttgacggtg tgtatctgcc gttccataaa gcaaaccaat
    37501 tcctcggtat ggaaccgctg ccgacattta tcgctaatga cgtgataaaa atgcctgatg
    37561 ttccccgcta tactgaagaa tatcgcaagc atcttgtgga aatttttggt taactagagc
    37621 tcaggcttta gaaggagtta accatgctta ccgtaatcgc agaaatccgt actcgtcctg
    37681 gtcaacatca ccgtcaggcg gtattggatc agtttgctaa aatcgttcca accgtactga
    37741 aagaagaagg ttgccacggc tatgcgccaa tggtggattg cgctgctggc gtgagtttcc
    37801 agtctatggc accggattct atcgtgatga ttgagcagtg ggaaagcatc gcgcaccttg
    37861 aagcgcatct gcaaaccccg cacatgaagg cgtacagcga agccgtaaaa ggcgacgtgc
    37921 tggagatgaa tatccgtatt ctgcagccag ggatttaatc ctgccttgtt tgcccggcca
    37981 tcctgaccgg gcaatgttct ttcctttaaa cctcaatctc cgccatgtcg cctttctctt
    38041 gcaaccagtt gcggcgatct tccgagcgtt tcttcgccag cagcatatcc atcatcgcgt
    38101 cagtacgctg atcgtcttca tcatcgatag tcaactgcac cagacggcga gtgttcggat
    38161 caagcgtggt ttcacgcaat tgcatcgggt tcatttcccc cagaccttta aaacgctgga
    38221 cgttcggctt gcctttcttg cgttttaatt gctcaagtac gccctctttc tcttcttccg
    38281 tcagcgcgta ataaacctct ttcccgagat caatacggta gagcggtggc agtgcgacgt
    38341 aaacgtgacc gtgtttcacc aacgcgcgga agtgttttac gaacaaagcg cagagcagcg
    38401 tggcaatgtg cagaccatca gagtccgcat ccgcgaggat acagattttg ccataacgaa
    38461 gctggctcag atcgtcgctg tcaggatcga taccgatcgc taccgaaata tcatgcactt
    38521 cctgcgaggc cagcacttcg tcggaagaaa cttcccaggt gttaaggatc ttacctttca
    38581 gcggcatgat cgcctgatat tcacgatcgc gcgcctgctt ggcagatccg cctgcggagt
    38641 caccttccac gaggaacagt tcggtacggt taaggccctg cgcggtacaa tcagccagtt
    38701 tgccaggcag cgccgggccg ctggtcagct ttttacgcac cactttttta gccgcacgca
    38761 tacgacgctg ggcgctggaa atcgccatct ctgccagcaa ctccgccgcc tgaacgttct
    38821 ggttcagcca caggatgaag gcatctttca ccacgcccga aacgaatgcc gcacactgac
    38881 gcgaagagag acgctctttg gtctgcccgg caaactgcgg atcctgcatt ttaaccgaca
    38941 gaacataggc gcagcgatcc cagatatctt ccgccgacag cttcacgccg cgcggcagaa
    39001 tgttgcggta ttcacagaac tcacgcatcg cgtccaacag gccctgacgc agaccattaa
    39061 catgggtacc gccctgcatc gttgggataa ggttgacgta gctttcggtc agcagttcac
    39121 cgccttccgg cagccacagt agcgcccagt ccacagcttc agtatcgcca gcgaaattac
    39181 cgataaacgg tttttctggc agcgtcggta aaccgtttac cgcttccgcc aggtaatcat
    39241 tcagaccgtc ctgatagcac cagcgctgtt cggtgttgtt gatctcatct ttaaaggtaa
    39301 tctcaacgcc ggggcacagt actgctttgg ccttcagcac atgcgtcagg cgtgaaacag
    39361 aaaaacgcgg gctgtcaaag aaggtttcat ccggccagaa gtgcacgctg gtaccggtat
    39421 tgcgtttacc gcaagtgccg acaacctgta aatcctgcac cttttcgcca ttttcaaagg
    39481 cgatgttata aacctgacca tcgcggcgca cgttaacttc tacgcgcttc gacaaggcgt
    39541 taactaccga aatccccacg ccatgcaggc cgccagagaa ctggtaattt ttgttagaga
    39601 atttaccgcc tgcatgcaga cggcaaagaa tcagttcaac cgccggtact ccttcttccg
    39661 ggtgaatatc caccggcatc ccgcgcccat cgtcaatcac ttctaacgac tggtcagcat
    39721 gtaagattac atccacgcgt tttgcgtgac ccgccagtgc ttcatcgaca ctgttatcaa
    39781 taacttcttg ccccaaatgg ttagggcgag tggtatcggt atacatcccc ggacggcggc
    39841 gaaccggctc aagcccggtg agtacctcaa tggcatcagc gttataagtt tgcgtcatgg
    39901 tttaagttag caattcgagt tgatcgtcag agatggtgca gaccaagaaa atcgacgatc
    39961 gggttgaaat aatcttcgaa gcccgtgaat gcgtggttgc cgccttctat gacagtctgg
    40021 cggcaggaag catagtacgc caccgcctgg cggtaatcca gcacttcatc tcccgtctgt
    40081 tgcagcagcc agatcaaatc cggcgcttcc agcgggtcaa tctgcatgac tttgagatcg
    40141 taaatatggc gtgactctag cacatattgc tgcccggtgt aggggttctc gttctgaccg
    40201 agatagtccg tcagcaattc aaacgggcgt accgccgggt ttaccaccac tgcgggcagc
    40261 ataaaacatt gcgacaacca ggtggcgtaa tatcccccca gtgacgaacc gacaataccc
    40321 agcgaatcgc cgccatgttc caggacaatg gattccagca gttctgccgc gtcggaagga
    40381 tacggcggca actgcggaat gatcatctca acgtcagggt gatgttccgc cagccagttt
    40441 tttaacaagc tcgcttttgc agagcgcggc gagctgttga aaccgtgtaa ataaagaagc
    40501 gtagacatca gtagccttct gaagcggtat caggttggaa acgtgtgtcc gccaggcgat
    40561 gcacctcggt ggtcagcgtg ccatcagcat gtaactcgag agtacgccag ccgggcgcga
    40621 tagtatccag cgtaaagttg gaacagtgcg gcttaaactg cacacaggtc gacggcgttg
    40681 ccagcaggcg gcgaccgttc cagtcgagat ccagctcctg atgaatatga ccgcacagca
    40741 agtatttgac gtgcggaaac ttcgccagca cggtatccag ttcgcccgcg ttacgcagac
    40801 tgtgttgatc gagccaacta caacccgcag gtagcggatg atgatgcagc agcagcaacg
    40861 tatggcgttc tggcgcatcg gccagtttac gttccagcca ctcaagctga aactcgctca
    40921 gctcaccgtg cggcacgcca aacacctggc tatccagcaa caggatttgc cattgctcac
    40981 caataaacac ccgcttcgcc ggggagatac ccgcatcctg taacgcgctg tacatcgcgg
    41041 gctggaaatc gtggttgccc ggcagccaga cgcagggcgc acgaaaactt gcgatgcctt
    41101 cagcgaaatg ctgataggcc gcagaggatt gatcctgcgc taaatcacct gtcgcgacaa
    41161 tcaggtcgaa ttcgtgctgg tgtggacgaa tcgcctccag caccgcctgg tagctttccc
    41221 aggtgtttac ccctaacagg gcttcgtgct tttgtgcaaa caggtgagta tcggtaattt
    41281 gtaaaatcct tcctctggcc tcaccagcca gaggaagggt taacaggctt tccaaatggt
    41341 gtccttaggt ttcacgacgc tagtaaaccg gaatcgccat cgctccatgt gctaaacagt
    41401 atcgcaacca gtccgctaaa aactggttaa tttgatgctt ttcgtcgcgt tgatgcaact
    41461 ttttattagg ataatcatac cgcgctttga agcgaaaaat ctgctggctt gaacacactt
    41521 cagccaccat cgcgtcatga tacagacgca ccgtcattga cggaaggctc cagtaactga
    41581 tcgcgggcgc agtctgttct attgtcacca gggtagtgta tcgggtcgat tccacaatcg
    41641 tcagccgata ttgtgcgttt gccacctgat agcttacagt ttcgccgggt gcgtcattgc
    41701 gcggtaacaa acggcgcaat tgtgaaaagt tcatctcgca caggcgcatc atttcaggaa
    41761 agtcaggtgt gtaacgcttc atttatgccc actcattttt taacgcttga tgatgcagct
    41821 gcagccattg caaagcgatg accgacgctg cgttgtcgat tttcccctct tctacccact
    41881 ggtatgcctg ttcccggctt accacatgaa cgcgaatatc ttcgttttca tcagccagac
    41941 catgaatacc gcttgcggtc gtggcgtcca cttcgcccac cataattgac gaacgctcac
    42001 tggtgccccc cgggcttgcc aggaaactta acaccggttt ggtccgtttg actatcagtc
    42061 ccgcctcttc aatcgcttcg cgacgggcaa catcttccac actttcaccc tcttcaatca
    42121 tcccggcaac catctccagt agccagggtg tttcgctggt gtcgtacgcg gcaatccgaa
    42181 tctgctcaat cagcacaact tcatcacgca ctgggtcaaa gggtagcaag actgcggcgt
    42241 gaccgcgctc aaaaatttcc cgccgtacct catgactcat ttgcccgttg aatagacgat
    42301 gacgaaatct ataaagatct aatgaaaaaa agccgcgata aagtgtttct cgtgcaataa
    42361 tttctacatc gtttttgcca aatgtaacgg gcaggttgtc tggcttaagc attgttaatg
    42421 tcctagcact aatagtgaat taaatgtgaa tttcagcgac gtttgactgc cgtttgagca
    42481 gtcatatgtt aaattgaggc acattaacgc cctatggcac gtaacgccaa ccttttgcgg
    42541 tagcggcttc tgctagaatc cgcaataatt ttacagtttg atcgcgctaa atactgcttc
    42601 accacaagga atgcaaatga agaaattgct ccccattctt atcggcctga gcctttctgg
    42661 gttcagttcg ttgagccagg ccgagaacct gatgcaagtt tatcagcaag cacgccttag
    42721 taacccggaa ttgcgtaagt ctgccgccga tcgtgatgct gcctttgaaa aaattaatga
    42781 agcgcgcagt ccattactgc cacagctagg tttaggtgca gattacacct atagcaacgg
    42841 ctaccgcgac gcgaacggca tcaactcgaa cgcgaccagt gcgtccctgc agttaactca
    42901 atccattttt gatatgtcga aatggcgtgc gttaacgctg caggaaaaag cagcagggat
    42961 tcaggacgtc acatatcaga ccgatcagca aaccttgatc ctcaacaccg cgaccgctta
    43021 tttcaacgtg ttgaatgcta ttgacgttct ttcctataca caggcacaaa aagaagcgat
    43081 ctaccgtcaa ttagatcaaa ccacccaacg ttttaacgtg ggcctggtag cgatcaccga
    43141 cgtgcagaac gcccgcgcgc agtacgatac cgtgctggcg aacgaagtga ccgcacgtaa
    43201 taaccttgat aacgcggtag agcagctgcg ccagatcacc ggtaactact atccggaact
    43261 ggcggcgctg aatgtcgaaa actttaaaac cgacaaacca cagccggtta acgcgctgct
    43321 gaaagaagcc gaaaaacgca acctgtcgct gttacaggca cgcttgagcc aggacctgga
    43381 gcgcgagcaa attcgccagg cgcaggatgg tcacttaccg actctggatt taacggcttc
    43441 taccgggatt tctgacacct cttatagcgg ttcgaaaacc cgtggtgccg ctggtaccca
    43501 gtatgacgat agcaatatgg gccagaacaa agttggcctg agcttctcgc tgccgattta
    43561 tcagggcgga atggttaact cgcaggtgaa acaggcacag tacaactttg ttggtgccag
    43621 tgagcaactg gaaagcgcac atcgtagcgt cgtgcaaacc gtacgttcct ccttcaacaa
    43681 cattaatgct tctatcagta gtattaacgc ctacaaacaa gccgtagttt ccgcgcaaag
    43741 ctcattagac gcgatggaag cgggctactc ggtcggtacg cgtaccattg ttgatgtgtt
    43801 ggatgcgacc accacgctgt acaacgccaa gcaagagctg gcgaatgcgc gttataacta
    43861 cctgattaat cagctgaata ttaagtcagc cctgggtacg ttgaacgagc aggatttgct
    43921 ggcactgaac aatgcgctga gcaaaccggt ttccactaat ccggaaaacg ttgccccgca
    43981 aacgccggaa cagaatgcta ttgctgatgg ttatgcgcct gatagcccgg cacccgtcgt
    44041 tcagcaaaca tccgcacgca ctaccaccag taacggtcat aaccctttcc gtaactgatg
    44101 acgacgacgg ggcttcggcc ccgtctgaac gtaaggcaac gtaaagatac gggttatctg
    44161 ccgcattctt cccccttctc gcttcaattt cgaccagcca tcctctattc tgatgggtat
    44221 ttaccactgg tcccggaaga caaaaatgaa acggacaaaa tccatacgcc acgcatcgtt
    44281 ccgcaaaaac tggagcgcac gccatctgac accagtcgct ctcgcggttg ccactgtttt
    44341 tatgctggct ggctgtgaaa agagtgatga aacagtgtct ctctatcaaa atgctgacga
    44401 ctgttcagct gcaaacccag gcaaaagcgc cgaatgtacc accgcgtaca acaatgcgct
    44461 gaaagaagcc gaacgtactg cgccgaaata cgccacccgt gaagactgtg ttgctgaatt
    44521 tggtgaaggt cagtgtcagc aagcaccagc ccaggctggc atggcaccag aaaaccaggc
    44581 gcaggctcag caatccagcg ggagtttctg gatgccgctg atggccggtt acatgatggg
    44641 gcgtctgatg ggcggcggcg cgggatttgc acagcagccg ctgttctcct cgaaaaaccc
    44701 ggccagtccg gcttacggta aatataccga cgcgacgggt aaaaactatg gcgcagccca
    44761 gccaggccgc accatgaccg taccgaagac ggcaatggca ccaaaaccgg cgaccaccac
    44821 taccgttacc cgtggcggtt ttggtgaatc tgttgccaaa caaagcacta tgcagcgtag
    44881 cgcaaccggc acctcttctc gttcaatggg tggctgatac cgatggaaag agtcagtatt
    44941 accgagcgcc cggactggcg tgagaaagcc cacgaatacg gtttcaattt tcacaccatg
    45001 tacggcgagc cgtactggtg tgaagatgct tactacaagt tgaccctcgc ccaggttgaa
    45061 aagctggaag aagtcaccgc cgaactgcac cagatgtgcc tgaaagtggt ggaaaaagtg
    45121 atcgccagcg atgagctgat gaccaaattc cgcataccaa aacacacctg gagttttgtg
    45181 cgtcaatcat ggctgactca ccagccatcg ctttattcgc gtcttgatct ggcgtgggat
    45241 ggcactggcg aacctaaact tctggaaaat aacgccgata cgccaacgtc actatacgag
    45301 gcggcgttct ttcagtggat ctggctggaa gatcagctta acgccggtaa cttgccggag
    45361 ggcagcgacc agtttaacag tctgcaagaa aaactgatcg atcgcttcgt tgagctgcgt
    45421 gaacagtatg gcttccagtt gctgcatctc acctgctgtc gcgacacggt ggaagatcgc
    45481 ggaaccattc agtatttgca ggactgcgca accgaagctg aaatcgccac cgagttcctc
    45541 tacatcgacg agataggttt aggcgaaaaa ggccagttca cggatttaca ggatcaggtg
    45601 atttctaacc tgttcaagct ttatccgtgg gaatttatgc tgcgcgaaat gttctccacc
    45661 aagctggaag atgctggcgt acgctggctg gaaccggcgt ggaagagcat tatctccaac
    45721 aaggcacttc taccgctact gtgggagatg ttcccgaatc acccgaacct gctgcccgct
    45781 tattttgcgg aagatgatca tccgcaaatg gaaaaatatg tggttaaacc gatcttctcc
    45841 cgtgaaggcg caaacgtgtc gatcattgag aacggcaaaa ccattgaagc agcggaaggt
    45901 ccgtatggcg aagaagggat gattgttcag caattccacc cgttaccgaa attcggcgac
    45961 agctatatgc tgattggtag ctggctggtg aacgatcaac ctgccggaat tggcattcgt
    46021 gaagaccgtg cgttgatcac ccaggatatg tctcggtttt atccacatat ttttgttgaa
    46081 taagccacga taccggatgg cactcgccat ccggtaattg ttagcctatc tgcaccgaca
    46141 gcatacttaa actgcccatt tctataccat cgaccggaat ggtaattggc tcctgcccat
    46201 cccacacacc taacacatac aacaacggca gatagtgctc tggcgttggg ttcgataacg
    46261 cgccaccttc atggtcgaga taattcacca gaggatgctg ctctaccggc ccttgccacg
    46321 tcagattcgc tttcacatac tcattaaacg acgtcgccca cggatacggt gaactatcac
    46381 cgtgccactt cactgtgcgc aggttatgca ccacgttacc gctggcgacc aacattattc
    46441 cttcatcacg cagcgccgcg agtttgcgcc ccatttcgaa atgccaggcg gcaggtttgc
    46501 tactgtcgat actcaactgc accataggga tatcggcgtc aggatacatc ttaatcagca
    46561 cgccccacga gccgtggtca aagccccagg cttctttatc cagcgtcacc gggatcggcg
    46621 ctaacagctc aaccagacgc tgtgccagcg caggcgaacc cggagcagga taatgcgtat
    46681 cgtacagcgc ctgcgggaag ccaccaaagt catgaatcgt gggcggcgtc tccatcgcgg
    46741 tcactcctgt tccacgggta aaccagtgag ccgaaaccac cacaatcgct tgcgggcgtg
    46801 gcaatgtcat ccccaacttc tgccagctgc gggtatacaa attatcttcc agcacgttca
    46861 tcggactacc gtgacctaaa aacaatgctg gcatacgtgt tgaagacatg atgatatcct
    46921 taactaaagg tgtcattttg atatcctcac aatacgcttg ttcggcggag taagaacccg
    46981 gataacaatg atgatgatca tcagttattt tgacgatctg cctgaaggtg aagatttata
    47041 aggagttgtc gatgtcagta cctctcattc tgaccatact ggcgggggca gccacgttta
    47101 ttggcgcgtt tctcggcgtt ctcggacaaa aaccctcgaa ccgcttactg gcgttttcgc
    47161 taggttttgc ggcggggatc atgttgctca tctcattaat ggaaatgctt cctgccgcac
    47221 tagcagctga aggaatgtcg ccggtgttgg gttatggaat gtttatcttc ggtctgcttg
    47281 gctactttgg cctggaccgc atgttgccgc atgcgcatcc gcaggattta atgcaaaaat
    47341 cggtgcagct gttgccaaaa tcgatcaagc gcacagccat tctgctcact ctcggcatca
    47401 gtctgcataa cttcccggaa gggattgcca cctttgtcac ggcgagcagc aacctggagc
    47461 tgggatttgg catcgcactg gccgtcgcgt tgcacaatat ccctgaaggt ctggcagtgg
    47521 caggcccggt ttatgcggca acggggtcta aacgcaccgc gattctgtgg gcggggattt
    47581 ctggactagc agaaattctt ggtggtgtgc tggcgtggtt aatcctcggt agcatgattt
    47641 ccccggtggt catggcggca atcatggcgg cggttgcagg aattatggtg gcgctctcgg
    47701 ttgatgaatt aatgccgctc gccaaagaga ttgaccctaa taataacccc agctatggcg
    47761 tactatgtgg aatgtcagtg atgggattca gtttagtgct gctacaaacg gcgggaattg
    47821 gttaaaaaca acatcagaag ttgtccagca catggacaac ttctgccagc ttcacgaaag
    47881 ccttattcta ccttttcggc tgtctcttct ctcgtactgt ttaagtattt gttccgcgct
    47941 tcaagcagtg cgtctctgtc ctggcgaaac gcccgacaat aacgtttcaa atgcaggaaa
    48001 taacccacca caaccagagc aatcaataca atccagtacc aggcgataaa catcctttta
    48061 cccttaatat ttaacaatac taaacattta gcgcataaat ttcacatatc ctttttcgga
    48121 tatattcata tggtcgcata ataacggaac aattatatga ttttgatcat aatgaaaaag
    48181 aatgcctgat attaatgaaa ttttctcata gaaagagaag cggaattaac atcttgcata
    48241 gcagggcaaa ataaaaaccg aagccagcta caacttcggt ttcttgatta aggcagtaat
    48301 taagcagcgg ttttcagctg gctttacgct catgtgcctg acggtatgcc accaggtctt
    48361 caatagtcac gagcgccata ttgtgtttat tggcaaactc aatgcactct ggtgcacgcg
    48421 ccatcgtgcc atcgtcatta gtcagctcac acagcacacc agccggttta aagcctgcca
    48481 gcgtcatcaa atcaatagtc gcttcagtat gaccgccacg cgtcagtaca ccacctgcct
    48541 gcgcgcgaag tgggaaaacg tggcccgggc gattcagatc agaaggtttt gcgccatcgg
    48601 caatcgctgc gcgaacggtc gtaatacgat cagcggcaga aacaccggta gtcacacctt
    48661 cagccgcttc aatggtcacg gtaaaaccgg tgccataggc gctggtgtta ttttctacca
    48721 tcattggcag atcaagttgt ttacggcgat cttcagtgat gcacaggcaa acaataccgc
    48781 taccgtggcg aatggtcagc gccatctgct caacggtcat ggtttctgcc gggaagatca
    48841 tatcgccttc gttttcacgg tcttcatcat caagcaccat tacaccgcgt ccttcacgca
    48901 gcgcagccag tgcattttca acacgttcga aaggcgtacc aaaagaggaa agtagcgtct
    48961 gattcatggt aaaaaaacct cactaaaatt atggttacca gaatcagggc agtcttagga
    49021 gtggcggcat atagccaaaa taacgtgagc gggtccatgc ccgacagaat cgttactctc
    49081 tcccatccgg actctaaccg tcggccccgg aattacaccg gatctgctgt cctttgagtt
    49141 cgcacccaaa gcgctcgcgg gctttcaact gagttgattt accgccggtg gggaatttcg
    49201 ccccgccctg agaataagcg gattcactat aacgctaatg attagcggca gcaacgcata
    49261 gcttcacata attctggttt atgacttacc cttatcgcac tacaatggta ctcaacacct
    49321 atcagtacag ggaaactaca atgattgacc cgaaaaaaat tgagcaaatc gctcgccagg
    49381 ttcacgaatc aatgcctaaa ggaatcaggg agttcgggga agatgtggag aaaaaaatcc
    49441 gccaaaccct acaagctcag ctgacgcgcc tcgaccttgt aagccgtgaa gagttcgacg
    49501 tccaaacgca agtcctgtta cggactcgtg agaaactggc gctgcttgaa caaagaatca
    49561 gcgaactgga aaatcgcagt actgagatca aaaagcagcc agatccagag actctccccc
    49621 caacgctgta aacatttgtc tttaatgcac tgtaaattat gaattaatcc tctaatttac
    49681 agtgttttct attacattat cgcttaatcc cattcagagt atcatcacaa aaatgaatga
    49741 ttgcaattga aaatataatt gttatgtatt ctttaagttt tacttaatcg ata
//
LOCUS       DADLTY010000006        43896 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.006, whole genome shotgun sequence.
ACCESSION   DADLTY010000006 DADLTY010000000
VERSION     DADLTY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43896
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.006"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(153..1328)
                     /gene="ubiF"
                     /locus_tag="JN334_000300"
     CDS             complement(153..1328)
                     /gene="ubiF"
                     /locus_tag="JN334_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAY7436574.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1474..2898
                     /gene="miaB"
                     /locus_tag="JN334_000301"
     CDS             1474..2898
                     /gene="miaB"
                     /locus_tag="JN334_000301"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAY7436575.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3012..4091
                     /locus_tag="JN334_000302"
     CDS             3012..4091
                     /locus_tag="JN334_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAY7436576.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYLAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AWEEAEQKRKAALAAERKREEQEQK"
     gene            4088..4555
                     /gene="ybeY"
                     /locus_tag="JN334_000303"
     CDS             4088..4555
                     /gene="ybeY"
                     /locus_tag="JN334_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAY7436577.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4645..5523
                     /gene="corC"
                     /locus_tag="JN334_000304"
     CDS             4645..5523
                     /gene="corC"
                     /locus_tag="JN334_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HAY7436578.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5548..7086
                     /gene="lnt"
                     /locus_tag="JN334_000305"
     CDS             5548..7086
                     /gene="lnt"
                     /locus_tag="JN334_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAY7436579.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            7483..8391
                     /gene="gltI"
                     /locus_tag="JN334_000306"
     CDS             7483..8391
                     /gene="gltI"
                     /locus_tag="JN334_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HAY7436580.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8561..9301
                     /gene="gltJ"
                     /locus_tag="JN334_000307"
     CDS             8561..9301
                     /gene="gltJ"
                     /locus_tag="JN334_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HAY7436581.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            9301..9975
                     /gene="gltK"
                     /locus_tag="JN334_000308"
     CDS             9301..9975
                     /gene="gltK"
                     /locus_tag="JN334_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HAY7436582.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            9975..10700
                     /gene="gltL"
                     /locus_tag="JN334_000309"
     CDS             9975..10700
                     /gene="gltL"
                     /locus_tag="JN334_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HAY7436583.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            10818..11753
                     /gene="rihA"
                     /locus_tag="JN334_000310"
     CDS             10818..11753
                     /gene="rihA"
                     /locus_tag="JN334_000310"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HAY7436584.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            11837..13507
                     /locus_tag="JN334_000311"
                     /pseudo
     CDS             11837..13507
                     /locus_tag="JN334_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(13567..15018)
                     /gene="djlC"
                     /locus_tag="JN334_000312"
     CDS             complement(13567..15018)
                     /gene="djlC"
                     /locus_tag="JN334_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HAY7436585.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(15015..15722)
                     /locus_tag="JN334_000313"
     CDS             complement(15015..15722)
                     /locus_tag="JN334_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY7436586.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            15824..16378
                     /locus_tag="JN334_000314"
     CDS             15824..16378
                     /locus_tag="JN334_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7436587.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(16388..17815)
                     /locus_tag="JN334_000315"
     CDS             complement(16388..17815)
                     /locus_tag="JN334_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HAY7436588.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(17812..18519)
                     /locus_tag="JN334_000316"
     CDS             complement(17812..18519)
                     /locus_tag="JN334_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY7436589.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            18683..19660
                     /locus_tag="JN334_000317"
     CDS             18683..19660
                     /locus_tag="JN334_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HAY7436590.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(19730..20212)
                     /locus_tag="JN334_000318"
     CDS             complement(19730..20212)
                     /locus_tag="JN334_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HAY7436591.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            20447..23029
                     /gene="leuS"
                     /locus_tag="JN334_000319"
     CDS             20447..23029
                     /gene="leuS"
                     /locus_tag="JN334_000319"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAY7436592.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            23044..23625
                     /gene="lptE"
                     /locus_tag="JN334_000320"
     CDS             23044..23625
                     /gene="lptE"
                     /locus_tag="JN334_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HAY7436593.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            23625..24656
                     /gene="holA"
                     /locus_tag="JN334_000321"
     CDS             23625..24656
                     /gene="holA"
                     /locus_tag="JN334_000321"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAY7436594.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            24658..25299
                     /gene="nadD"
                     /locus_tag="JN334_000322"
     CDS             24658..25299
                     /gene="nadD"
                     /locus_tag="JN334_000322"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAY7436595.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            25323..25934
                     /locus_tag="JN334_000323"
     CDS             25323..25934
                     /locus_tag="JN334_000323"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HAY7436596.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            26194..26511
                     /gene="rsfS"
                     /locus_tag="JN334_000324"
                     /gene_synonym="rsfA"
     CDS             26194..26511
                     /gene="rsfS"
                     /locus_tag="JN334_000324"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAY7436597.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            26515..26982
                     /gene="rlmH"
                     /locus_tag="JN334_000325"
     CDS             26515..26982
                     /gene="rlmH"
                     /locus_tag="JN334_000325"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAY7436598.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            27013..28914
                     /gene="mrdA"
                     /locus_tag="JN334_000326"
     CDS             27013..28914
                     /gene="mrdA"
                     /locus_tag="JN334_000326"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HAY7436599.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            28917..30029
                     /gene="mrdB"
                     /locus_tag="JN334_000327"
                     /gene_synonym="rodA"
     CDS             28917..30029
                     /gene="mrdB"
                     /locus_tag="JN334_000327"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HAY7436600.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            30040..31128
                     /gene="rlpA"
                     /locus_tag="JN334_000328"
     CDS             30040..31128
                     /gene="rlpA"
                     /locus_tag="JN334_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HAY7436601.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSITPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            31267..32478
                     /gene="dacA"
                     /locus_tag="JN334_000329"
     CDS             31267..32478
                     /gene="dacA"
                     /locus_tag="JN334_000329"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HAY7436602.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            32589..32852
                     /locus_tag="JN334_000330"
     CDS             32589..32852
                     /locus_tag="JN334_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HAY7436603.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            32953..33594
                     /gene="lipB"
                     /locus_tag="JN334_000331"
     CDS             32953..33594
                     /gene="lipB"
                     /locus_tag="JN334_000331"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAY7436604.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            33853..34806
                     /locus_tag="JN334_000332"
     CDS             33853..34806
                     /locus_tag="JN334_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAY7436605.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            35015..35980
                     /gene="lipA"
                     /locus_tag="JN334_000333"
     CDS             35015..35980
                     /gene="lipA"
                     /locus_tag="JN334_000333"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAY7436606.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(36081..36284)
                     /gene="tatE"
                     /locus_tag="JN334_000334"
     CDS             complement(36081..36284)
                     /gene="tatE"
                     /locus_tag="JN334_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HAY7436607.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(36413..37201)
                     /locus_tag="JN334_000335"
     CDS             complement(36413..37201)
                     /locus_tag="JN334_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HAY7436608.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            37294..37677
                     /gene="crcB"
                     /locus_tag="JN334_000336"
     CDS             37294..37677
                     /gene="crcB"
                     /locus_tag="JN334_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAY7436609.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(37731..37940)
                     /gene="cspE"
                     /locus_tag="JN334_000337"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(37731..37940)
                     /gene="cspE"
                     /locus_tag="JN334_000337"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY7436610.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(38115..38675)
                     /locus_tag="JN334_000338"
     CDS             complement(38115..38675)
                     /locus_tag="JN334_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HAY7436611.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            39264..40649
                     /gene="dcuC"
                     /locus_tag="JN334_000339"
     CDS             39264..40649
                     /gene="dcuC"
                     /locus_tag="JN334_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY7436612.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(40690..41031)
                     /locus_tag="JN334_000340"
                     /pseudo
     CDS             complement(40690..41031)
                     /locus_tag="JN334_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(41369..41878)
                     /locus_tag="JN334_000341"
     CDS             complement(41369..41878)
                     /locus_tag="JN334_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7436613.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            42041..42286
                     /locus_tag="JN334_000342"
     CDS             42041..42286
                     /locus_tag="JN334_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7436614.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            42261..43892
                     /locus_tag="JN334_000343"
                     /pseudo
     CDS             42261..43892
                     /locus_tag="JN334_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        11051 a        10971 c        10954 g        10920 t
ORIGIN      
        1 attaaaaaag ctcgcttcgg cgagcttttt gcttttctgc gttcattcaa tgtcgaatgc
       61 gatgttgaca cgtcttatcc ttcaatgtcg gatgcgacgc tgccgcgtct tatccgacct
      121 acggctggca cgcatccggc aatgttgtaa ggctacaacc ctaacgcata tttcagcgcc
      181 tgacgtttca acacgccagc acgctctgcc gccattaacc cgagattacg cacaaaacgc
      241 agcggcggca gattattact gaatccggca taaaacagat ccataccact ttgcataatg
      301 aagttatccg ccatgcgccg catctggtaa cgcttgagga caggataact ggcccacgct
      361 tcaccgtagc tgcgggcatt caccagaaca tcaatcaggg catcgacatc acgataacca
      421 agattcaccc cctgccccgc cagcggatgg atggtatgcg cggcatcgcc caccagcgca
      481 agccccggct gcacatactg caacgcatgt cggcgcgtca gcggaaacgc accagcggca
      541 agcggtgtaa cgtaacccag acgcgacggg aaatgcttcg cgatttccgc ctggagttgc
      601 gccatattca tattctgcaa ctggcgaata cgcgtcggag agtcatacca caccagcgat
      661 gcccagttat caaacaacgg cagaaacgca cgcggtccgt ccggggtaaa ttgctgccag
      721 gtgctgtcgc cgggatcgtt ctcacactgt acgctaatca acatgcacga ctgcgcatac
      781 tgccaggcat gaacgccaat tcccgccatc tgccgcacct gagaatttgc gccgtcggca
      841 ccaatcacca gtttcgcgcg gatcgtttca ccgcccttca gttccagctc ctgaagatca
      901 ttatctcgat gtaatgcaat cagcgaggtt ggcacacgta acgttacttt cggatgcgct
      961 tccagcgcct gccacaacgc ctgttgcagg actgtgtttt ccaccatgta gccaagcaac
     1021 ggcaacttta attcggctgc gtcaaatacc acatgtgccg tttcccactc ccaggtttcc
     1081 agtctgcggt aaggatggca acgcatagcc tgtactgcat cccagacccc taacccttta
     1141 agcaatgata ccgaagccgc gctgatcgcc gagatccgca cgtccggttg gctgtcagcg
     1201 acaaacggtg caggttctgc gtgctcgatc accgttaccg aaaatccgtg ctgtgccagc
     1261 cccagcgcca gtgcgccgcc gaccattcct ccgccgacaa tggcaatttc cgttggttga
     1321 tttgtcatgg tcaatcatcc tgttagcaat atgctttaag tttacaggat ttttaccccc
     1381 aagggctgat aacgctcata ctggtcacaa cggcagcaaa gcattacact atgcgccctg
     1441 cattcctggc tactatttcg caagagcaag tcgatgacca aaaaactcca tattaaaacc
     1501 tggggctgtc agatgaacga gtacgattca tcgaagatgg ccgatctgct ggatgccacc
     1561 cacggctatc aactgaccga cgtggcggaa gaagcggatg tgctactgct gaacacctgc
     1621 tcaatccgcg agaaggctca ggaaaaagtc ttccatcagt tgggtcgctg gaaactgtta
     1681 aaagagaaga atccagacct gattatcggc gtcggtggct gcgtggcatc gcaagaaggc
     1741 gagcacattc gccagcgcgc ccactatgtc gatattattt ttgggccgca aacgctgcac
     1801 cgtctgccgg agatgatcaa ctccgtgcgc ggcgaccgca gcccggttgt agatatcagc
     1861 ttcccggaaa tcgagaagtt tgaccgtctg ccggaaccgc gcgccgaagg gccgaccgcg
     1921 tttgtctcca tcatggaagg ctgcaataaa tattgcacct actgcgtggt gccttacacc
     1981 cgtggtgaag aggtaagccg tccgtctgac gatattctgt ttgagattgc ccagcttgcg
     2041 gctcagggcg tgcgtgaagt caacctgctc ggtcagaatg tgaatgcctg gcgtggtgag
     2101 aactacgacg gcaccaccgg atcgtttgcc gatctgttgc gtctggttgc tgcgatcgac
     2161 gggatcgatc gtattcgctt taccaccagc catccgatcg aattcaccga cgatatcatc
     2221 gaagtgtacc gcgacacgcc tgagctggtg agcttcctgc atctgccggt acagagcggt
     2281 tccgatcgca ttctgaacct gatgggacgt acccatacgg cgctggagta caaagcgatc
     2341 atccgtaaac tgcgtgcggc gcgtccggat attcagatca gttctgactt cattgttggc
     2401 ttccctggcg aaaccaccga agacttcgag aaaacgatga agctgattgc cgacgtcaat
     2461 ttcgacatga gctacagctt tatcttctct gcacgtccag gaacgcctgc cgccgatatg
     2521 gttgatgatg ttccggaaga agagaagaag cagcgtctgt atattctgca agaacgtatc
     2581 aatcagcaag cgatggcatg gagccgtcgg atgctcggca ccacccagcg tattctggta
     2641 gaagggactt cgcgtaagag catcatggag ctttccgggc gtaccgaaaa taaccgcgtg
     2701 gtcaacttcg agggcacacc ggatatgatc ggtaaattcg tcgatgtaga aattaccgac
     2761 gtctacccga actctctgcg cggtaaagtg gtgcgaactg aagatgaaat gggtctgcgc
     2821 atggcagaaa caccggagtc ggtgattgcc cgcacccgca aagaaaacga ccttggcgtg
     2881 ggttattatc agccgtaatt ctcaggcctg ccgttccggt gggccttgtt ttttctctct
     2941 ttatccccat ctttatcgca atgcttgcgc ctttttcctg tggcgtgaat aatttcctta
     3001 taggccggtg aatgttcagc ttccggcggc atacagataa gaggaacggt ttgaacatag
     3061 acactcgcga aatcaccctg gagccagcag acaatgcgcg tctgttgagc ctgtgcggcc
     3121 cgtttgatga caacatcaag cagctcgaac gccgtctcgg catcgagatc aatcgccgcg
     3181 ataaccactt taaactgacc ggccgtccga tttgcgtcac cgctgcggca gacattctgc
     3241 gtagcctgta tgtcgatact gccccgatgc gcggtcagat tcaggatatc gaaccggaac
     3301 agatccacct tgcgattaaa gaagcgcggg tactggagca aagcgcggag agcgtgccgg
     3361 agtacggcaa agcggtcaat atcaaaacta aacgcggcgt aattaagccg cgcacgccaa
     3421 accaggcgca gtacatcgcc aatattctcg accatgacat taccttcggc gttggcccgg
     3481 cgggtacggg taaaacctac ctggcagtgg ctgcggcagt tgatgccctg gagcgtcagg
     3541 aaattcgccg tattctgctg actcgtccgg cggtcgaagc cggtgagaaa ctgggcttcc
     3601 tgcctggcga tttaagccag aaagtagacc cgtatctgcg cccactgtac gacgcgctgt
     3661 ttgaaatgct gggctttgag aaagtcgaga aactgattga gcgcaacgtt attgaagtcg
     3721 cgccgctggc ctatatgcgt ggtcgtacgc tgaacgacgc gtttatcatt ctcgatgaga
     3781 gccagaacac taccatcgaa cagatgaaga tgttcctgac ccgtatcggt tttaactcaa
     3841 aagcggttat caccggcgac gtcacacaga tcgacctgcc gcgtaatact aaatcaggct
     3901 tacgtcatgc cattgaagtg ctggccgatg tcgaagagat cagctttaac ttcttccaca
     3961 gcgaagacgt ggttcgtcac ccggtggtgg cgcgtatcgt taacgcttat gaagcctggg
     4021 aagaagcaga acaaaaacgt aaagcggcgc tggcagcaga acgcaagcgc gaagaacagg
     4081 aacaaaaatg agtcaggtga tcctcgattt acaactggca tgtgaagata attccgggtt
     4141 accggaagag agccagtttc aaacatggct gaatgcggtg atcccgcagt ttcaggaaga
     4201 gtcggaagtg actattcgcg tggtcgatac cgccgaaagc cacagtctga atctgaccta
     4261 tcgcggtaag gataagccga ccaacgtgct ctccttcccg tttgaagtgc cgcctggcat
     4321 ggagatgtcg ctactgggcg atctggttat ctgccgtcag gtggttgaga aggaagctca
     4381 ggagcaaggc aaaccactgg aggcgcactg ggcgcatatg gtggtgcacg gcagtctgca
     4441 tttgttaggt tacgatcaca ttgaagatga cgaagcagaa gaaatggaag ccctcgaaac
     4501 agagattatg cttgctctgg gctatgagga tccgtacatt gccgagaaag aataatttgc
     4561 caggcatttg actggcagac gtccccgccg ttggtgatta acgacggcgt tttgattaac
     4621 taacatgaga acccatacga cgccatgagc gacgacaatt cacacagtag tgacacgata
     4681 agcaacaaga agggattttt ctccctgtta ctcagccaac ttttccacgg tgaaccgaaa
     4741 aaccgtgacg aactgctggc gctgatccgt gattccgggc agaacgacct tatcgacgaa
     4801 gatacgcgcg atatgctcga aggggtgatg gacatcgcag accaacgcgt tcgcgacatc
     4861 atgatccccc gctcccagat gattaccctg aaacgcaacc agacgctgga cgaatgcctt
     4921 gatgtcatca tcgagtccgc ccactcacgt ttcccggtga ttagcgaaga caaagatcac
     4981 attgaaggga ttctgatggc gaaagacttg ctgccgttta tgcgcagcga tgctgaagcc
     5041 ttcagcatgg acaaagtgtt acgtcaggcg gttgtcgttc ctgaaagtaa gcgcgtagac
     5101 cggatgctga aagagtttcg ctctcagcgt taccacatgg cgatcgttat tgacgaattc
     5161 ggtggggttt ccggtctggt gaccattgaa gacatcctgg aactgattgt tggtgagatt
     5221 gaagacgagt atgacgaaga agatgatatc gacttccgtc agctgagtcg tcatacctgg
     5281 accgtgcgcg cactggcttc cattgaagac ttcaacgaag cgttcggcac ccactttagc
     5341 gatgaagaag tcgacactat cggtggtctg gtgatgcagg cgtttgggca tcttccggcg
     5401 cgtggcgaaa ctatcgacat cgacggttac cagttcaaag tggcgatggc cgatagtcgg
     5461 cgtattattc aggttcatgt caaaatcccg gatgactcac cccagccaaa gctggatgaa
     5521 taaaaccgaa actggataga taactacatg gattttgcct cattaattga acgccagcgc
     5581 attcgcctgc tgctggcgtt attattcggt gcctgcggaa cgctggcctt ctctccttac
     5641 gacgtctggc ctgcggcgat tatttcgctg atggggcttc aggcgttgac ctttaaccgt
     5701 cgcccactcc agtctgccgc tattggcttt tgctggggat ttggcctctt tggcagcggt
     5761 attaactggg tctatgtcag catcgcgacc tttggcggga tgcctggccc ggttaacatc
     5821 ttcctggtgg tgctgctggc ggcgtatttg tcgctgtata ccggactgtt tgctggcgtg
     5881 ctgtcgcgtc tgtggccgaa aaccacctgg ctgcgcgtgg cgattgccac ccctgctctc
     5941 tggcaagtga ccgagtttct gcgcggttgg gtgctgactg gcttcccgtg gttacagttc
     6001 ggctatagcc agattgatgg cccgttaaaa gggctggcac cgctaatggg cgtggaagcc
     6061 attaacttcc tgctgatgat ggttagtggc ctgctggcac tggcgttggt caaacgcaac
     6121 tggcgtccgc tggtggtggc cgtcgtgctg tttgccctac ccttcccgct gcgttacatc
     6181 caatggttta ccccgcaacc ggagaaaacc attcaggttt cgatggttca gggcgatatt
     6241 ccgcaatcgc tgaaatggga cgaaggccag cttcttaata cgctaaagat ttactacaac
     6301 gcaacggcac cgctgatggg caaatcatcg ttgattatct ggccggagtc ggcgataacc
     6361 gatctggaaa ttaatcagca accgttcctc aaagcactgg acggtgagtt gcgtgataaa
     6421 ggtagctcgc tggtaaccgg gattgtcgac gcgcgtctca ataagcagaa ccgctacgat
     6481 acctacaaca ccatcatcac gctgggtaaa ggtgcaccgt acagctacga atcagccgat
     6541 cgctataaca aaaaccatct ggtgccgttt ggcgagtttg tcccgctgga gtcgattctg
     6601 cgtccgttag caccgttctt tgatctgccg atgtcgtcgt tcagccgtgg gccatatatc
     6661 cagccgccgc tgtcggcaaa tggtattgag cttactgcgg ctatttgcta cgagattatt
     6721 ctcgccgagc aagtgcgcga taacttccgc ccggataccg actacctgct gactatctcc
     6781 aacgatgcgt ggtttggtaa atctattggt ccatggcaac acttccagat ggcgcgaatg
     6841 cgtgcgctgg agctggcgcg cccactgttg cgcagcacca acaacggcat tacggcggtg
     6901 attggcccgc agggtgagat tcaggcgatg atcccgcagt tcgcccgcga ggtgttaacc
     6961 actaacgtga cgccgaccac cggactcaca ccatacgcac gtaccggcaa ctggccgctg
     7021 tgggtgctga cggcactgtt tggttttgct gctgtgttga tgagtctgcg tcagcgacgt
     7081 aaataatccc ttcttatcgt gccggaatac atcccatatt tcggcacgct caccggatgc
     7141 ggcatcagtg cattatccgg ataacaatat tctcctatcc aactttggca catctattgc
     7201 tttgttatac aaggcaaagc ttgaaccagc atcagtacaa cccggttgca ccacagcaat
     7261 ccaccggtag caccaaaaga gtgcatttta agaattttgc ttcagaatgg tgcggtgcac
     7321 ctcgcaaaaa caaacaataa aacatcaata tctttacatt tagccaaact aaatgttacc
     7381 taacaggcac aacactgcac aataaagttg cagacgataa caacacaaac actcacaacg
     7441 ggtatccatg cgttcttaac gcagaagata aaggtgttgg atatgcaatt acgtaaacct
     7501 gccacagcaa tcctcgccct ggcgctttcc gcaggactgg cacaggcaga tgacgccgcc
     7561 ccggcagcag gcagtacgct ggacaaaatc gccaaaaacg gtgtgattgt cgtcggtcac
     7621 cgtgaatctt cagtaccttt ctcttattac gacaatcagc aaaaagtggt gggttactcg
     7681 caggattact ccaacgccat tgttgaagca gtgaaaaaga aactcaacaa accggacttg
     7741 caggtaaaac tgattccgat tacctcacaa aatcgtattc cactgctgca aaacggcact
     7801 ttcgattttg aatgtggttc taccaccaac aacgtcgaac gccaaaaaca ggcggctttc
     7861 tctgacacta ttttcgtggt cggtacgcgc ctgttgacca aaaagggcgg cgatatcaaa
     7921 gattttgccg acctgaaagg caaagccgta gtcgtcactt ccggcactac ctctgaagtt
     7981 ttgctcaaca aactgaatga agagcaaaaa atgaatatgc gcatcatcag cgccaaagat
     8041 cacggtgact ctttccgcac cctggaaagc ggtcgtgccg ttgcctttat gatggatgac
     8101 gctctgctgg ccggtgaacg tgcgaaagcg aagaaaccag acaactggga tatcgtcggc
     8161 aagccgcagt ctcaggaagc ctacggttgc atgttgcgta aagacgatcc gcagttcaaa
     8221 aagctgatgg atgacactat cgctcaggtg cagacctccg gtgaagcgga aaaatggttt
     8281 gataaatggt tcaaaaatcc aattccgccg aaaaacctga acatgaattt cgaactgtca
     8341 gacgaaatga aagcactgtt caaagaaccg aatgacaagg cactgaacta attacaagaa
     8401 ccaggggcgg aaattccagc cctctcgatt gttacgtagc acggacagac tatacgcctg
     8461 atggtcgttc cccatcgggc ctgaaaaccg caatacgctg ggtaacaatc ttcgagggta
     8521 gcagttaacg ctgctaccct cttttttctg gagtagattt atgtctatag actggaactg
     8581 gggtattttt ttacaacaag ccccgttcgg caataccacc tatcttggtt gggtctggag
     8641 tggttttcag gtgacgatcg ctttgtcgat ctgcgcctgg attatcgctt tcctcgtcgg
     8701 ttcatttttc ggcattttac gtaccgttcc aaaccgtttt ctctccggtc tgggtacgtt
     8761 gtatgtcgaa ctgttccgca acgtgccgtt gattgtgcaa ttctttacct ggtatctggt
     8821 gatcccggag ctgctgccgg agaaaatcgg catgtggttt aaggccgagc tggatcccaa
     8881 tattcagttt ttcctttcat ccatgctctg cctggggctg tttaccgccg cccgtgtttg
     8941 cgaacaggta cgcgccgcta ttcagtcgct accgcgtggg cagaagaatg ccgcgctggc
     9001 gatgggcctg acgctaccgc agacttatcg atacgtactg ctgcctaatg cttatcgcgt
     9061 tatcgtcccg ccgatgactt ccgagatgat gaacctggtg aaaaactccg ccatcgcctc
     9121 gaccatcggt ctggtggata tggcggcgca ggcgggtaaa ttgctcgact actcggctca
     9181 cgcctgggag tcgtttaccg ccatcacgct tgcgtatgtg ttgattaacg cttttatcat
     9241 gctggtgatg acgctggttg aacgtaaagt tcgcctgccc ggcaacatgg ggggcaaata
     9301 atgtacgagt ttgactggag ttccattgtt ccttccctgc catatctgct cgacgggctg
     9361 gtgatcaccc tgaaaatcac cgtcacggca gtcgtgattg gtattttgtg gggtaccatg
     9421 ctggcggtga tgcgcttgtc cagcttcgcg cctgtttcgt ggtttgccaa agcctacgtt
     9481 aacgtattcc gctcgattcc tttagtcatg gttttgctgt ggttttactt gattgtgccg
     9541 ggatttctgc aaaatgtgct gggattatcg ccaaaaaatg atattcgttt aatttcggcg
     9601 atggtggcgt tttcgatgtt tgaagcggcc tactattcag agattatccg tgccggtatt
     9661 caaagtattt cccgtggtca gtcgagcgcc gcgctggcgt tggggatgac tcactggcag
     9721 tcgatgaaac tgattattct gccgcaggct ttccgcgcga tggtgccgct gctgctcact
     9781 cagggcatcg tactgttcca ggatacctcg ctggtgtatg tgttatctct ggccgatttc
     9841 ttccgtaccg cctcaaccat tggtgagcgt gatggtacgc aggtcgagat gatcctgttt
     9901 gccggatttg tttatttcgt tattagcctt agcgcgtcgt tgttggtcag ctacttgaaa
     9961 agaaggacag cataatgatt accctgaaaa atgtttcaaa atggtatggt cactttcagg
    10021 tgctgaccga ctgctcaacc gaagtgaaaa aaggcgaagt ggtggtggtt tgcggcccgt
    10081 ctggttccgg caaatcaacg ctgattaaaa ccgtcaacgg cctcgaacca gtgcagcaag
    10141 gtgaaatcac cgtcgatggt atcgtggtta acgacaagaa aaccgatctg gcaaagctgc
    10201 gctcccgcgt cgggatggta ttccagcatt tcgagctgtt ccctcatctg tcgattatcg
    10261 aaaacctgac cctggcgcag gtgaaagtgc ttaaacgcga taaagcgccc gctcgtgaaa
    10321 aagcattgaa attgctggag cgcgtgggac tttccgcaca tgccaataaa tttccggcgc
    10381 agctttccgg cggtcagcag cagcgcgtgg caatcgctcg cgcattgtgt atggctccta
    10441 ttgcgatgct gttcgacgaa ccgacatcgg cgctggatcc ggagatgatc aacgaagtgc
    10501 tggacgtgat ggtggaactg gcgaacgaag gaatgaccat gatggtggtg acccacgaaa
    10561 tgggctttgc ccgcaaagtg gcgaatcggg tgatctttat ggacgagggt aaaattgtcg
    10621 aagactcgcc gaaagacgct ttcttcgatg atccgaaatc ggaccgcgca aaagacttcc
    10681 tcgcgaaaat cctgcattaa tcactctggc gcacataccc aattgcgcgc catctcacgc
    10741 tttatcgttt cgcccctcgt atctgttaca tcctggcgtt acccttgtcg caaagaagca
    10801 cacaacaagg agcaacaatg gcactgccaa ttctgttaga ttgcgaccca ggtcatgatg
    10861 acgctatcgc aatagttctc gcactcgcct caccagagct tgatgtcaaa gcaattacgt
    10921 cttccgccgg aaaccagaca ccagaaaaaa ccttacgcaa tgttctgcgt atgctgacct
    10981 tgcttaatcg caccgatatt ccggtggcag gcggcgcggt aaaaccgtta atgcgtgagt
    11041 tgattatcgc ggacaatgtg cacggcgaaa gcggtctcga cggcccggca ttaccggaac
    11101 cgacgttcgc accgcaaaac tgtacggcgg tggagctgat ggcgaaaacg ctgcgcgaaa
    11161 gtgcggaacc tgtcacgatt gtttctaccg gaccgcaaac taacgttgcc ttgctgctca
    11221 atagccatcc ggaactgcat agcaaaattg cccgtatcgt gattatgggc ggcgcaatgg
    11281 ggcttggtaa ctggacgcct gcggctgaat ttaatattta cgttgacccg gaagcggcag
    11341 aaattgtctt ccagtcaggg atcccggtgg tgatggccgg tctggatgtt actcataaag
    11401 cgcaaatcca cgttgaagac accgagcgtt tccgcgcgat tggcaaccct gtttcaacca
    11461 ttgttgccga actgctggat ttcttcctcg aatatcataa agacgaaaaa tggggctttg
    11521 tcggcgcacc gctgcatgac ccatgcacca tcgcctggct gttgaaaccc gagttgttta
    11581 ccactgttga gcgctgggtt ggcgtggaaa cacaggggaa atatacccag ggtatgacgg
    11641 ttgttgatta ttattatctg actggcaata aaccgaatgc cactgtaatt gtcgatgttg
    11701 atcgtcaggg ctttgttgat ttactggctg atcgtctgaa attttacgct taacactcgt
    11761 cattgattaa gatcaccacg cgttacctgg gtggtcttaa tctatttatc tctttttctt
    11821 tcaagatata aatgcaatgg ataatgcaga actcgccata ggtatcgatc tcggcactac
    11881 caatagttta attgccgtct ggaaagacgg tgccgcgcaa ttaattccaa ataagttcgg
    11941 tgaatattta acaccatcca taattagcat ggatgaaaat aatcatattt tagtcggaaa
    12001 accggctgta tcacggcgta cttcgcatcc ggataaaacg gcagcgttat ttaaacgtgc
    12061 aatgggcagt aataccaact ggcggttagg cagcgacaca tttaacgcgc cagaactgtc
    12121 ctctttggta ttacgctcat taaaagaaga tgccgaagaa tttctgcaac gtccgattaa
    12181 agatgtggtg atctccgttc tggcttattt cagcgatgaa caacgcaagc atacccgttt
    12241 agcagcggac ttagccgggt taaatgcggt acgcttaatt aatgaaccca cagcagctgc
    12301 gatggcgtat ggcctgcata cccaacaaaa tacccgttcg ctggtgtttg atctcggtgg
    12361 cggcacgttt gacgttacgg tgcttgagta cgccacgccg gtgattgaag ttcacgcctc
    12421 cgctggcgac aacttcctcg gtggcgaaga ttttacccat atgttggtcg atgacgtttt
    12481 aaagcgcgcg gatgtcacaa ggaccacgct taacgagagt gaactggcag ccttgtacgc
    12541 ctgtgtggaa gcggcaaaat gtagcaatca atcgccaatg cacattcgct ggtagtacca
    12601 ggatgaaacg cgggaatgcg aattttacga gaacgaactg gaagatttgt ggttgccgct
    12661 gctcaatcgc ttgcgagtgc cgattgaaca ggcgttgcgc gatgcgcgtc tgaagccgag
    12721 tcaaatcgac agtctggtgc tggttggcgg cgcgtcacaa atgccgctgg tgcagcgaat
    12781 cgccgtgcgt ctgtttggca aattaccgta tcaaagttac gatccgagca ccattgtcgc
    12841 gctgggcgca gcaatccagg ccgcctgccg cttacgcagt gaagatattg aagaagtgat
    12901 cctcactgat atttgccctt actcgttggg cgttgaggtt aaccgccagg gcgtttccgg
    12961 cattttctcg ccgattattg aacgaaacac cactgtgccc gtgtcgcgtg tagaaactta
    13021 ttcaaccatg cacccggaac aggattcaat tacggttaac gtctatcagg gagaaaacca
    13081 caaagttaaa aacaacattc tggtggaatc cttcgatgtg ccgttgaaga aaaccggggc
    13141 ttatcagtcg attgatattc gctttagtta tgatatcaac gggttgcttg aagttgacgt
    13201 gcttctggaa gacggcagcg ttaagtccag agtgattaac cacagcccgg taacattgag
    13261 cgcgcagcag attgaagaga gtcggacgcg gttatccgca ttgaaaattt atccgcgcga
    13321 tatgctcatc aatcgcacct ttaaagccaa actggaagag ttgtgggcgc gggcgctggg
    13381 tgacgagcga gaagagattg gccgggtgat caccgatttt gatgcggcgc tgcagtcaaa
    13441 cgatatggcc cgcgtcgatg aggtcaggcg gcgggcgagc gtttatttag ccattgagac
    13501 ctcataatca cggctgccgg atgcggcgta aatgctgcat ccggcatcgg cgcaaaaaac
    13561 acatcgtcaa ctaaacaaca tcgcgatttt gccgagagca taacctataa ccagcaccgt
    13621 taaactgatt ttgataatgc tggtaatgct ctgtggtttt tcaccctcat catttttttc
    13681 ttcatggttt tcaggtgttg gctcatcgac atcttcatcc tccggctcga gttttacttt
    13741 cgtcaacagc atccgggtga taatggcctc taatggatga gcatctccgc cttccagcct
    13801 tgtattaaga cgggccagca tctcttgttt cggaaacgct tcaagtacta cctgttccgc
    13861 tacccgatac aaatccagta acggtgaatc ttcttctgtc tcgctaccga ctctcgattt
    13921 ccaccacgcc catgctttgc gtttgaagaa taacgactca ataaacgatt gacctaacgg
    13981 cgataatttt ccgctatgta aaatttccgc ccataacagc cgcgtccggg cgctctcacc
    14041 aggaacatat ttcagcggtt gaccttgcgc atctaccatg ctccgcgctt cgataaccat
    14101 cactaataac gcgaaataat ccgggcaatg acgcttgctc cactgcaaca acaaattatc
    14161 agcttgtgta gatgggtaac tttcccagta cgcgccgaga tcagcaagca ggctttcccc
    14221 ctcgccgcag tacaaacgtt gtgcacactg gtaataccgg gcatcttcgc tttctggttc
    14281 ttccgcttgc cactgtttgg ctacaggaat gagttccacc atacctgttt gcaccgagct
    14341 gtaccagcgc aacagtttgc gatgtaactt tgcatcatca ggaatgaacc acggtccttc
    14401 catcgccaaa aacgcagtga catagtcagg atggtaacgg cagcaacgtt ccagcgcgaa
    14461 gtaatattca acagtctggt tctgtaacgc gacgggatat tccagaaggc taaggaaatc
    14521 gaaacaatca ccagcctgaa tggcctcaag aaaagcctcc ctttcttcct catccattcc
    14581 ttcgctgtca ttaaacgaat gccagttcaa acgctctgcc aacaaagaag cacaatttaa
    14641 tgacagatat cgcgcttcta tagcgattgc acacagcggc cagcgtaatt gatcgacatc
    14701 ctccatgttc caggtattta attgctgaat aaatttctgc caggcggagg gctgaaaacg
    14761 atcactttct gaatccagta atgtcctgaa tgcacgtagt atttcatgtt cagcggtggc
    14821 atctttttct tcatcatctg cttcctcgac aggatttacc gccagtcgca gcgcctcttc
    14881 ataggcctgg cgtaatgctt taaacccctg cggatcggtt tcgggatgac acaacggtaa
    14941 gcgagcaaga taagcctggc ggataatgtc tatttgcgtc gtgctttcaa tttcgaggat
    15001 ttgccaacat gttttcatag gtcgctcaca tccttaagcg ttaccgggca ttcaggctgg
    15061 acagttaatg gctgccaggg aagcgtggca tagaaacggt cgcttcccgc tgccagctct
    15121 tcgaacatcc gactcccggc gctatcgtat ttgtacaggg ttagcgcctc gcgtagcgaa
    15181 gcgttgtctt cgcactgaga agattgccag tagagacgcc ctagtgaata ggcagaaaaa
    15241 tagtgcatcc agctgtgata gtaatggtga gctcgtgcat agacgcgcga ttgcagccat
    15301 aaactctctt cttccgtcag ccagttgttg agcacaccgg cccgtgacag aaatcccatt
    15361 cgaacataat cccaggcttt gattccccct tctccacagc acatagcggt ttgggcaacg
    15421 aattgggtat aaatcaaccc tctctccgag ccaccagcaa ttaatgcttt ccactcttca
    15481 ggggaagagc gttgccatgt cagatataag ccagctaaat cattggcatg accatcatca
    15541 gtcatctgat aaatagtctg aattaaatca tcacgattat ttattcccca ggatgatgag
    15601 agatcgaatt cttttatccc ggtatacatt tttggcgagt catgcgcggg tttgcaactc
    15661 tgattaagta tttccatggg cgcagaaagc gcaaaaagaa gatattgttc ttccttattc
    15721 attttttaat aatcccttaa gtgcattaac tgactacttt tttattattg atgagtataa
    15781 acttgacgct ttcaaaataa aaagaaaatc gaagcattca cacatgaata aaaaattaat
    15841 gtatatattc gcaattttta tagttgcagc aattacctgt attagccaac ccaagaaaac
    15901 gatgttgcgt gataaagcca tggtgaatta tgcctttgat tatttaagat caccaggcag
    15961 tctaccgtta accacggcag ccacggagct ttccgcgatt catggtcact caacatcgca
    16021 atatcgcctt ggcgaatttt atcttcatgg tagcgacggt aaaccactgg attatacaca
    16081 ggcgagatac tggtatgagc aatcagcaga acaggaaaat ccgcgcgcac aaagcaaact
    16141 ggggtggatc tacctgaaag gcctgggagt caaacccgat acccgtaaag caattctctg
    16201 gtataaggaa gcagctgaac aagggtatgc tcatgctcaa tatactttgg gtttgatcta
    16261 cagaaatggc actggtatta atgttaacca ttatgaatct caaaaatggt taaaactggc
    16321 cgccaaacaa cattacaaaa atgcggaaag attacttgcc ggacttcccg cacattaacg
    16381 tcgactgtca cccgaagaga tggagtattt tccctatcaa accggcaaag ataaaaatgt
    16441 agaaaataat tttgatgacg cccagacagc ccagcccgcc atcatcaacg ggcattggct
    16501 gcggttgcgg ctccggctcg acggtttcct gaccggtaaa attcgccgcc tgaattaata
    16561 atcgacgaat aattaatgct tccagtggac aacgatcttc tgcatcaagg cggctcacaa
    16621 tcggcattaa tgattcttct gtcggttggc tggcctgcac taattgtttg gcaactaaat
    16681 ataatggcca taagggatgt tctgtatcac gcgtgagtga gatccgcggc ttacgtcctt
    16741 tgtgcaaaac catctcgacc agtgaacgcc ccagcgcaga gagtttttca tcgctgagta
    16801 tttcgtataa atgaaaacgc gtacgggcgc tgtcgccggg gacataaagt agcggttttc
    16861 cctgatcgtt aaccagatca cgcgcttcaa tcatcatcac cactaatggg taataataga
    16921 cccgatgctg acggcaccat tgcaacatta aagcatccgc ctgggtggag ggatactcgc
    16981 gccagtagta acaaagttct gccagcaggc tttcgccttc tccacaatag acgcgttgtg
    17041 cgtattcata ataacgcgca tcttcattat caggttcagc ttcccgccac gaccgggcgt
    17101 aatccaggag ttcggggatt cccaggcggc ttgcgctata ccagcggagt aaatttctgt
    17161 gcagttttgc atcatcgggg ataatcactg cgcgatgttg cgctaaatac aatgtcagcc
    17221 attctggatg cgatgaccaa atccgagcga acatttggta aaaatcgatg gtgtcatttt
    17281 gcacggcaac tggcagatgc agaatggtct ggaagttaaa aacattcccc ttcgcaatgg
    17341 catataaaaa ggattccagt tcttcttcgt ctatttcccc gacgttttcc tcctgcaacc
    17401 accgcaatct ttctgctaat aacaccacgc actcgaagga taaatgggca gtgttcatgg
    17461 cgattgtgca cagcgaccag cgtaattcat caatctcttc catcgagcaa taatttaatt
    17521 gctgaatgaa tcgctgccag gcggagggca gaaaacgttc gctatcagag gcaagtaacg
    17581 cgcgaaaggc gagcagaatt tcatgttctg ctacctcata ttcttctggt tgccaaacag
    17641 atttagcagg cgactgcgca atccgtagcg cctcctcata cgcctgacga agttgtttaa
    17701 aaccctgcgg atcggtttct ggatgaaagg acggtaacag cgccagataa gcgcggcgga
    17761 taatatcgac gtcagtcgtt tcctctatat cgaggatctt ccagcaattt ttcataagtc
    17821 gctcatatcc ttaagcgttt ccgggcatgt ggggtagtca gccagtggtt gccaggacaa
    17881 ggtagggtaa tacgcatcat cactggcaaa taattgataa aacatatcat tgccggaatc
    17941 gtcgtattct ttacgcgcta agaattcgcg taataaaggc agatgatcat cttcgggaga
    18001 ctgccaatat tgccgaccaa aggtataacc ggcaaagtat tgccgccagt tactataata
    18061 acgtagagcc cgtagatgaa tacgcgactg gatccataga ctttcttctt cactcaacca
    18121 gttattaagt acgcccattc gacttaaaaa acccatccgg acataatccc atgctttaat
    18181 tcctccctca ccacagcatt cggctgtgct ggcgacaaat tgcgcataag cctgaccttg
    18241 ctcatttaat tcagcaagat agtcacgcca ttcacatgga gaataacgaa accagcggtg
    18301 ataaaaccct gccaaccgag cggcatgacc gttctcgatc attcggtgaa ttatgcgcag
    18361 caggtcatca cggtcatcaa ttccccatga ggaagaaagg tcaaaaaagc atgtacctaa
    18421 atacattttg ggggatgaat gcgaaggtaa acagcattca ttgcgtattt ccatcggagt
    18481 ggaaagtgca aacaatattt tttgcgaccc catgtccata taatttatcc ttaaacattc
    18541 atttaatgaa tgtaaactac atttcgaggc tcagttattt atacttttct gacgcaacat
    18601 aatacgcata aaataaggct tacgcaaaaa tggaacacat aaaaatataa tatattttat
    18661 ctacaaccat aaggaaatga taatgatttt tacgtcaagt tgctgcgata atttatcaat
    18721 agatgagatt atcgaacgtg ctgaaaaagg cgattgcgag gctcaatata ttgttgggtt
    18781 ttattataat cgcgatagcg caattgactc tccggacgac gaaaaggcct tttactggct
    18841 gaagctggcc gctgagcaag gtcattgtga agcgcagtat tccttagggc aaaagtatac
    18901 cgaggataaa agccgtcata aagataatga gcaagccatc ttctggctga aaaaagctgc
    18961 cctacaaggc catactttcg cttccaacgc ccttggctgg atactggatc gtggagaaga
    19021 ccccaactat aaagaagcgg ttacctggta tcagatagcc gcggagagcg gaatgtctta
    19081 tgcgcaaaat aatcttggat ggatgtacag aaatggcaac ggagtcgcac aagactatac
    19141 gctggcattt ttctggtaca aacaagctgc attacaaggc catagttacg cacaagacaa
    19201 tctggccgat ctttataaag acggagaagg cgttgctcaa aacaagacac tcgccgcatt
    19261 ctggtatttg aaaagcgcac agcagggtaa tcggcacgcc cagtttcaaa ttgcatggga
    19321 ttataacgct ggcgaagggg tggaccagga ctataagcaa gcgatgtact ggtatctgaa
    19381 ggctgccgct caggaaagcg tcggcgctta cgtcaacatc ggttatatgt ataaacacgg
    19441 acaaggcgtt gagaaagatt atcaggctgc ctttgaatgg tttacgaaag ccgctgaatg
    19501 caatgacgcc actgcctggt ataacctggc cattatgtat cactacggag aaggaagacc
    19561 tgtcgatctc cgacaggctc tcgacctgta tcgtaaagtt cagtcatccg gaaccagaga
    19621 tgtcagtcag gaaattcgtg agactgaagc tttactgtag aaataaaaac aagggccggg
    19681 gaatttcatt ctgcccatat tctctttatt gctgtgcgaa atcagactat tacggctcaa
    19741 acgggcgtct ctggaactgg tcataaccac atttcgggca tagcgtcagc acttccggtg
    19801 tgtagatcgg gagatggaag tgacatttct cgcagaccag atttcccagc ccgaccactt
    19861 ctccgctgtg ataaacccca tgatgattga ggtcctggaa aatttcgcgc cattcaagct
    19921 gcgttttatc ggtgatgtct gccagctcct gccacaagct ttctttaatc acccgcataa
    19981 agacgctgtc agattcttct ttcaggctct cttcatagct catggcgaac tcttccaggt
    20041 cacgtctgac agctcgcgtc agctcatcga tctcggttcg cgttaactcc cctgttttta
    20101 ttacgcgctc gcgcgcctgt tccaccagtg cgtcgatatc acgttcgcca ttgcgcaggc
    20161 gttcgttcag tgacgcgacc agttcacggt aatattgagc aaccttgttc atcgtttcat
    20221 ctcccgggtc gttaactgac tataataata gacgttcttt gttccttgct ttgctaatac
    20281 gcccacaaac tctgtaaatt aatgtacgaa taatccgggc atcatttcaa cgtcgagtgc
    20341 gcgaaaggct gttttgacgc ggacgtttgg gctatgctat gcggatctga aaaaccacct
    20401 caacgctaca tttgtagccg tattgaaaac aggaccactg gctgccatgc aagagcaata
    20461 ccgcccggaa gagatagaat ccaaagtaca gcttcattgg gatgagaagc gcacatttga
    20521 agtaaccgaa gacgagagca aagagaagta ttactgcctg tctatgcttc cctatccttc
    20581 tggtcgacta cacatgggcc acgtacgtaa ctacaccatc ggtgacgtga tcgcccgtta
    20641 ccagcgcatg ctgggcaaaa acgttctgca gccgatcggc tgggacgcgt ttggtctgcc
    20701 tgcggaaggc gcggcggtga aaaacaacac cgctccggca ccgtggacgt acgacaacat
    20761 cgcgtatatg aaaaaccagc tcaaaatgct gggctttggt tatgactgga gccgcgagct
    20821 ggcaacctgt acgccggaat actaccgttg ggaacagaaa ttcttcaccg agctgtataa
    20881 aaaaggcctg gtatataaaa agacttctgc ggtcaactgg tgcccgaacg accagaccgt
    20941 actggcgaac gaacaagtta tcgacggctg ctgctggcgc tgcgatacca aagttgaacg
    21001 taaagagatc ccgcagtggt ttatcaaaat cactgcttac gctgacgagc tgctcaacga
    21061 tctggataaa ctggatcact ggccagacac cgttaaaacc atgcagcgta actggatcgg
    21121 tcgttccgaa ggcgtggaga tcaccttcaa cgttaacgac tatgacaaca cgctgaccgt
    21181 ttacactacc cgcccggaca cctttatggg ttgtacctac ctggcggtag ctgcgggtca
    21241 tccgctggcg cagaaagcgg cggaaaataa tcctgaactg gcggccttta ttgacgaatg
    21301 ccgtaacacc aaagttgccg aagctgaaat ggcgacgatg gagaaaaaag gcgtcgatac
    21361 tggctttaaa gctgttcacc cattaacggg cgaagaaatt cccgtttggg cagcaaactt
    21421 cgtattgatg gagtacggca cgggcgcagt tatggcggtt ccgggtcacg accagcgcga
    21481 ctacgagttt gcctctaaat acggcctgaa catcaaaccg gttatcctgg cggctgacgg
    21541 ctctgagcca gatctttctc agcaagccct gactgaaaaa ggcgtgctgt tcaactctgg
    21601 cgagttcaac ggtcttgacc atgaagcggc cttcaacgcc atcgccgata aactgactgc
    21661 gatgggcgtt ggcgagcgta aagtgaacta ccgcctgcgc gactggggtg tttctcgcca
    21721 gcgttactgg ggcgcgccga ttccgatggt gacgctggaa gacggtaccg taatgccaac
    21781 cccggacgac cagctgccgg tgatcctgcc ggaagatgtg gtgatggacg gcattaccag
    21841 cccgattaaa gcagatccgg agtgggcaaa aactaccgtt aacggtatgc cagcgctgcg
    21901 tgaaaccgac actttcgaca cctttatgga gtcctcctgg tactatgcgc gctacacttg
    21961 cccagagtac aaagaaggta tgctggattc cgaagcggct aactactggc tgccggtgga
    22021 tatctacatt ggtggtatcg aacacgccat tatgcacctg ctctacttcc gcttcttcca
    22081 caaactgatg cgtgatgcag gcatggtgaa ctctgacgaa ccagcgaaac agttgctgtg
    22141 tcagggtatg gtgctggcag atgccttcta ctatgttggc gaaaacggcg aacgtaactg
    22201 ggtttccccg gttgatgcta tcgttgaacg tgacgagaaa ggccgtatcg tgaaagcgaa
    22261 agatgcggca ggccatgaac tggtttatac cggcatgagc aaaatgtcca agtcgaagaa
    22321 caacggtatc gacccgcagg tgatggttga acgttacggc gcggacaccg ttcgtctgtt
    22381 tatgatgttt gcttctccgg ctgatatgac tctcgaatgg caggaatccg gtgtggaagg
    22441 ggctaaccgc ttcctgaaac gtgtctggaa actggtgtac gagcacacag caaaaggtga
    22501 tgttgcggca ctgaacgttg atgcgctgac tgaagatcag aaagcgctgc gtcgcgatgt
    22561 gcataaaact atcgctaaag tgaccgatga tatcggccgt cgtcagacct tcaacaccgc
    22621 aattgcggcg attatggagc tgatgaacaa actggcgaaa gcaccgaccg atggcgagca
    22681 ggatcgcgct ctgatgcagg aagcactgca ggccgttgtc cgtatgctca acccgttcac
    22741 cccgcacatc tgcttcacgc tgtggcagga actcaaaggc gaaggcgata tcgacaacgc
    22801 gccgtggccg gttgctgacg aaaaagcgat ggtggaagac tccacgctgg tcgtggtgca
    22861 ggttaacggt aaagtccgtg ccaaaatcac cgtcccggtg gacgcaacgg aagaacaggt
    22921 tcgcgaacgt gctggccagg aacatctggt agcaaaatat cttgatggcg ttactgtacg
    22981 taaagtgatt tacgtaccag gtaaactcct caatctggtc gttggctaag cgcgggagga
    23041 agcgtgcgat atctggcaac actgttgtta tctctggcgg tgttaatcac cgccgggtgt
    23101 ggctggcatc tgcgtgacac cacgcaggtt ccttccacta tgaaggtcat gatcctggac
    23161 tcaggcgatc cgaacgggcc attaagccgt gcggtgcgta accagttacg tctgaatggt
    23221 gtcgagttgc ttgataaaga aaccacgcgt aaggacgttc catccctgcg tttgggtaaa
    23281 gtgagcatcg cgaaagatac cgcatcggta ttccgtaacg gtcaaacagc agagtatcag
    23341 atgatcatga cggtgaatgc gaccgtgttg atccccggcc gtgatatcta cccgattagc
    23401 gccaaagtct tccgttcatt cttcgataac ccgcaaatgg cgttagcgaa agataacgaa
    23461 caagacatga tcgtaaaaga gatgtacgac cgtgctgccg aacagctgat tcgtaagctg
    23521 ccaagcatcc gtgctgcgga tattcgttcc gacgaagaac agacgtcgac cacaacggat
    23581 actccggcaa cgcctgcacg cgtctccacc atgctgggta actgatgatt cggttgtacc
    23641 cggaacaact ccgcgcgcag ctcaatgaag ggctgcgcgc ggcgtatctt ttacttggta
    23701 acgatcctct gttattgcag gaaagccagg acgctgttcg ccaggtagct gcggcacaag
    23761 gattcgaaga acaccacact ttttccattg atcccaacac tgactggaat gcgatctttt
    23821 cgttatgcca ggctatgagt ctgtttgcca gtcgacaaac gctattgctg ttgttaccag
    23881 aaaacggacc gaatgcggcg atcaatgaac aacttctcac actcaccgga cttctgcatg
    23941 acgacctgct attgatcgtc cgcggtaata aattaagcaa agcgcaagaa aatgccgcct
    24001 ggtttactgc tcttgcgaat cgcagcgtgc aggtgacctg tcagacaccg gagcaggctc
    24061 agcttccccg ctgggttgct gtgcgcgcaa aacagctcaa cttagaactg gatgacgcgg
    24121 cgaatcaggt gctctgctac tgttatgaag gtaacctgct ggcgctggct caggcactgg
    24181 agcgtttatc gctgctctgg ccagacggca aactgacatt accgcgcgtt gaacaggcgg
    24241 tgaatgatgc cgcgcatttc accccttttc attgggttga tgctttgttg atgggaaaaa
    24301 gtaagcgcgc gttgcatatt cttcagcaac tgcgtctgga aggcagcgag ccggttattt
    24361 tgttgcgcac attacaacgt gaactgttgt tactggttaa cctgaaacgc cagtctgccc
    24421 atacgccact gcgtgcgttg tttgataagc atcgggtatg gcagaaccgc cggggcatga
    24481 tgggcgaggc gttaaatcgc ttaagtcagc cgcagttacg tcaggccgtg caactcctga
    24541 cacgaacgga actcaccctc aaacaagatt acggtcagtc agtgtgggca gagctggaag
    24601 ggttatctct tctgttgtgc cataaacccc tggcggacgt atttatcgac ggttgatatg
    24661 aaatctttac aggctctgtt tggcggcacc tttgatccgg tgcactatgg tcatctaaaa
    24721 cccgtggaaa cgctggcgaa tttgattggt ctgacgcggg tcacaatcat ccctaataat
    24781 gttcctccgc atcgtcccca gccggaagcg aacagcgtgc agcgtaaaca catgcttgaa
    24841 ctggcgattg ccgacaagcc attatttact cttgatgaac gcgagctaaa gcgcaatgcc
    24901 ccctcttaca ctgcgcaaac actgaaagag tggcggcagg aacaaggacc ggacgtgccg
    24961 ctggcgttta ttattggtca ggattcattg ctgacctttc cgacctggta cgaatacgaa
    25021 acgatactcg acaatgcaca tttgatcgtc tgtcggcgtc caggttaccc acttgaaatg
    25081 gcgcaaccgc aataccagca atggctggaa gatcatttga cacataaccc ggaagatctt
    25141 caccttcagc ctgccggtaa aatttatctg gctgaaacgc cgtggtttaa catctcggcg
    25201 accatcatcc gcgaacgttt gcaaaacggt gaatcgtgtg aggatttatt gccggaaccg
    25261 gtattgactt acattaacca acaaggcttg tatcgctgat acctgtcgtt acggagggca
    25321 tgatgcgact gtggttaatt cgtcatggcg aaacacaagc gaatgtcgat ggtctgtaca
    25381 gcggtcatgc gcccacaccc ctgaccgcac gcggtattga gcaagcgcaa aatctgcata
    25441 cactgctaga tgatgtttcc tttgacttgg ttttatgcag tgaactggaa cgggcacagc
    25501 ataccgcgcg actggttctc agtgaccgcc agctccccgt tcatatcatc cctgaactca
    25561 acgaaatgtt ttttggcgac tgggagatgc gacatcatcg cgacctcatg caagaagatg
    25621 ccgaaaacta tagcgcgtgg tgcaatgact ggcagcatgc tatccccacg aacggtgaag
    25681 gatttcaggc attttcgcaa cgtgtggaac gctttatcgc aaggcttagt gaatatcagc
    25741 actatcagaa tattttaatc gtcagccatc agggcgtact gagtctgtta attgctcgtt
    25801 taattggcat gcctgccgag tctatgtggc attttcgcgt tgaccagggg tgttggagcg
    25861 ccattgatat caaccaaaaa ttcgcgacgc tacgcgtcct caatagccgt gccatcgggg
    25921 tcgagaacgc atgacttttc tgttttttta cgggtaagcc gcaacgacca ttgacagccc
    25981 cgggcaggct gatattctcc gcagccagac tttttccgcc agacacgact ttgtagaaat
    26041 tgttttacaa aaatggcgat gcaatctgcg gcgcggggtg ggatgatagc ccactttcga
    26101 aagccgattc ggcgacaatt gtcccgaaat cgcctctggt tcaggtatac tgacagacca
    26161 tttttatcta tttgattcac ccagggggaa aacttgcagg gtaaagcact ccaggatttt
    26221 gttatcgaca aaattgatga cctcaaaggt caggacatca tcgccttaga cgttcaaggc
    26281 aaatccagca tcaccgactg catgatcatc tgtacgggta cgtccagccg tcatgttatg
    26341 tccattgctg accacgttgt gcaggagtct cgcgcagcgg gcctgttacc gctcggcgta
    26401 gaaggtgaaa acagcgccga ctggattgtc gtggatttgg gcgatgtgat tgtccatgtc
    26461 atgcaggaag agagccgtcg cctgtatgaa ctggaaaaac tctggagtta atgcgtgaag
    26521 ctgcaacttg tcgccgtggg aacgaaaatg ccggactggg tacaaaccgg ttttaccgag
    26581 tacctgcgtc gttttccgaa agatatgccc ttcgagctga ttgaaattcc ggccggcaaa
    26641 cgcggcaaga atgcggacat caagcgcata ctcgacaaag agggtgagca gatgttggcg
    26701 gccgcaggca aaaaccgcat tgtcaccctc gatattccag gcaagccctg ggatacgccg
    26761 cagttagccg ctgagctgga acgctggaag ctggatggtc gcgacgtaag tctactgatt
    26821 ggcgggcctg aagggttgtc gcctgcctgt aaagcggcgg ctgagcagag ctggtcgctg
    26881 tcggcgctta ccctccccca tccgctggtt cgcgtgctgg tcgcagagag tctgtaccgg
    26941 gcgtggagca tcaccaccaa ccatccttat caccgtgagt gataagggag ctttgagtag
    27001 aaaacgcagc ggatgaaact acagaactct tttcgcgact atacggcaga gtccgcgctg
    27061 tttgtgcgcc gggcgctggt cgcctttttg gggattttgc tgctgaccgg cgtgcttatc
    27121 gccaacctgt ataatctgca aattgttcgc tttaccgact accagacccg ctctaatgaa
    27181 aaccgcatta agctggtgcc tatcgcgccc agccgcggca ttatctacga ccgtaacggt
    27241 atccctctgg ccctcaaccg cactatctac cagatagaaa tgatgccgga gaaagtcgat
    27301 aacgtgcagc aaacgctgga cgctttgcgc agcgtggtag atctgaccga tgacgatatt
    27361 gctgcattcc gaaaagagcg cgcacgttca caccgtttca cctctattcc ggtgaaaact
    27421 aacctaaccg aagtacaagt agctcgcttt gccgtcaatc agtaccgttt tccgggtgtc
    27481 gaagttaaag gctataaacg tcgttactat ccttacggtt cggcgttgac ccacgtcatc
    27541 ggctatgtgt cgaaaatcaa cgataaagac gtcgaacgcc tgaataatga cggcaaactg
    27601 gccaactatg cggcaacgca tgatattggt aagctgggca ttgagcgtta ctatgaagac
    27661 gtgctgcacg gtcagaccgg ttatgaagag gttgaagtta acaaccgtgg gcgtgttatc
    27721 cgccagttaa aagaagtacc accgcaagcc ggacacgata tttacctgac gctggatctc
    27781 aaactccagc aatatattga aacgctgctg gcgggtagcc gcgcagctgt ggttgttacc
    27841 gaaccgcgta caggtggggt gctggcgctg gtttccacgc ctagttatga cccaaacctg
    27901 tttgttgacg gtatctccag caaagattat tccgccttgc tgaacgaccc gaatacaccg
    27961 ctggtgaacc gcgccacaca gggggtttat cctcccgcgt ctacagttaa accctatgtg
    28021 gcggtttcgg cattaagcgc cggggtgatc acgcgcaata cgacgctgtt tgacccaggc
    28081 tggtggcaac taccagggtc ggaaaaacgt tatcgtgact ggaaaaaatg gggccacggg
    28141 cgtctgaatg tcacaagatc gctggaagaa tctgcggata ccttcttcta tcaggtggcc
    28201 tacgatatgg ggatcgatcg cctctccgaa tggatgggta aattcggtta tggtcattac
    28261 accggtatcg acctggcgga agaacgttcc ggcaacatgc ctacccgcga atggaaacag
    28321 aaacgcttta aaaaaccgtg gtatcagggt gacaccattc cggttggtat cggccagggt
    28381 tactggacag cgaccccaat ccagatgagt aaggcactga tgatcctgat taatgacggt
    28441 atcgtgaagg ttccgcattt gctgatgagc accgccgaag acggcaaaca ggtgccatgg
    28501 gtacaaccgc atgaaccgcc cgtcggcgat attcattccg gttactggga gctggcgaaa
    28561 gacggtatgt acggtgttgc taaccgccct aatggtacgg cgaataaata ctttgccagc
    28621 gcaccgtaca aaattgcggc gaaatctggt accgctcagg tcttcggtct gaaagcgaac
    28681 gaaacctata atgcgcacaa aattgccgag cgtttacgtg accacaaact gatgaccgcc
    28741 tttgcgccat acaacaatcc gcaagtggct gtcgccatga ttctggagaa cggcggcgcg
    28801 ggtccggcgg ttggtacgct gatgcgccag atcctcgacc acattatgct gggtgataac
    28861 aacaccgatc tgcctgcgga aaatccagcg gttgccgcag cggaggacca ttaatcatga
    28921 cggataatcc gaataaaaaa acattctggg ataaagtcca tctcgatccc acaatgctgc
    28981 tgattttact ggcattgctg gtttacagcg ccctggttat ctggagcgcc agcggtcagg
    29041 atattggcat gatggagcgt aaaatcggcc aaatcgcgat gggtctggtc atcatggtgg
    29101 tgatggcgca aattcctcca cgcgtttatg aaggctgggc cccctatctc tatatcatct
    29161 gtattatttt gctggtggcg gtagatgctt tcggagccat ctctaaaggc gctcaacgct
    29221 ggctggacct cggtattgtt cgttttcagc cgtcggaaat tgccaaaata gccgtaccac
    29281 tgatggttgc gcgctttatc aaccgcgacg tttgcccgcc atcgttgaag aatactggca
    29341 tcgcgctggt gctgatattt atgcccacgc tgctggtggc tgcacagcct gacctgggaa
    29401 catcaatcct cgttgcgctt tccggtctgt ttgtactgtt cctctctggc cttagctggc
    29461 gtctgattgg cgtcgcagta gtgctggtag cggcgttcat tccgattctg tggttcttcc
    29521 tgatgcatga ttaccagcgc cagcgcgtaa tgatgctcct ggacccggaa tcagacccac
    29581 tcggcgcggg ctatcacatt attcagtcta aaattgctat tggctccggc ggattacgcg
    29641 gcaaaggctg gctgcacggc actcagtcac agcttgaatt tctccccgaa cgccatactg
    29701 actttatctt cgcggtactg gcggaagagc tgggattagt gggcattctg attctgctcg
    29761 ctctctacat tctgctgatc atgcgcgggc tgtggatagc cgccagagcg caaaccacct
    29821 ttggtcgcgt catggctggt ggcttaatgc tgatattatt cgtttatgtc ttcgtaaata
    29881 ttggtatggt aagcggtatt ctgccggttg taggggttcc gctcccactg gtcagttatg
    29941 gaggatcggc gctaattgtg ctgatggctg ggttcgggat tgtaatgtca atccacaccc
    30001 acaggaaaat gttgtcgaaa agcgtgtaag aggtgcgcaa tgcgtaagca gtggctcggg
    30061 atctgcatcg cggcaggaat gctcgcggca tgtacaagcg atgatggtca gcaacagacg
    30121 gtaagtgtac cgcagcctgc ggtatgtaac ggccccatag ttgaaattag cggggcggac
    30181 ccgcgtttcg aaccactgaa cgcgacggca aatcaggatt accagcgcga cggtaaaagc
    30241 tacaaaatcg tgcaggatcc gtctcgattt agccaggcgg gactggcggc aatctatgat
    30301 gccgaaccag gcagtaacct gacggcctct ggcgaagctt tcgatccgac aaagctgacg
    30361 gcggcccatc caacgcttcc gatccccagc tacgccagaa tcactaacct ggctaacggg
    30421 cgaatgatcg tggtgcgcat taatgatcgc ggcccttacg gcaacgaccg cgttatttcg
    30481 ctttctcgcg cggcagctga ccgtcttaac acgtcaaaca acaccaaagt tcgtatcgat
    30541 ccgattattg tcgcccagga tggttcgctt tctggtcctg gtatggcttg taccacagtc
    30601 gccaaacaga cttacgccct gcctgcacct cccgatttaa gcggtggcgc gggaacaagt
    30661 tcagtgtctg gcccgcaggg tgacattctt ccggtcagta attcgacgct aaaaagcgaa
    30721 gatccgaccg gcgcgccggt aaccagtagc ggtttcctcg gcgctccaac gaccttagcg
    30781 cctggtgtac tggaaggcag cgaaccgacg cctgcgccac agcccgttgt tacagcttcg
    30841 tcgataacgc ctgcaacctc gcctgcaatg gtgacaccgc aagccgcctc gcaaagcgcc
    30901 agcggcaact ttatggtgca agtcggggcc gtaagcgatc aggctcgtgc gcaacagtac
    30961 caacagcaac tgggacagaa gttcggcgtc cccggtcgcg taactcaaaa tggcgcggtc
    31021 tggcggatcc agcttggccc attcgccagc aaagccgaag ccagtacctt gcagcaacgt
    31081 ttgcaaaccg aagcccaatt acagtcattt attactaccg cgcagtagcg taaagcaggc
    31141 atctgatgtg tcaatttctg taaatgacgt taacaaagtc acgcggaaag tcagatgcct
    31201 gccggtagtg catttgctat agtagggcac ttttttaatt ccatcacgga tgtcgtagtt
    31261 cagaccatga ataccatttt ttccgctcgt atcatgaagc gcctggcgct caccacggct
    31321 ctttgcacag cctttatctc tgctgcacat gccgatgacc tgaatatcaa aactatgatc
    31381 ccgggtgtac cgcagatcga tgcggagtcc tacatcctga ttgactataa ctccggcaaa
    31441 gtgctcgccg aacagaacgc agatgtccgc cgcgatcctg ccagcctgac caaaatgatg
    31501 accagttacg ttatcggcca ggcaatgaaa gccggtaaat ttaaagaaac tgatttagtc
    31561 actatcggca acgacgcatg ggccaccggt aacccggtgt ttaaaggttc ttcgctgatg
    31621 ttcctcaaac cgggcatgca ggttccggtt tctcagctga tccgcggtat taacctgcaa
    31681 tcgggtaacg atgcttgtgt cgccatggcc gattttgccg ctggtagcca ggacgctttt
    31741 gttggcttga tgaacagcta cgttaacgca ctgggcctga aaaataccca cttccagacg
    31801 gtacatggtc tggatgctga tggtcagtac agctccgcgc gagatatggc gctgatcggc
    31861 caggcattga tccgtgacgt accgaatgaa tactcgatct ataaagaaaa agaatttacg
    31921 tttaacggta ttcgccagct gaaccgtaac ggcctgttat gggataacag cctgaatgtc
    31981 gacggcatca aaaccggaca cactgacaaa gcaggttaca atcttgttgc ttctgcgact
    32041 gaaggccaga tgcgcttgat ttctgcggtg atgggtggac gtacttttaa aggccgtgaa
    32101 gccgaaagta aaaaactgct aacctggggc ttccgtttct tcgaaaccgt taacccactg
    32161 aaagtaggta aagagttcgc ctctgaaccg gtttggttta gtgattctga tcgcgcttcg
    32221 ttaggggttg ataaagacgt gtacctgacc attccgcgtg gtcgcatgaa agatctgaaa
    32281 gccagctatg tgctgaacag cagtgaattg catgcgccgc tgcaaaagaa tcaggtcgtc
    32341 ggaactatca acttccagct tgatggcaaa acgatcgagc aacgcccgct ggttgtgttg
    32401 caagaaatcc cggaaggtaa cttcttcggc aaaatcattg attacattaa attaatgttc
    32461 catcactggt ttggttaaaa attgaacact tgaaagtgta atttccgtcc ccatatacta
    32521 agcatcagta aaaaaactcc cgccttctgg cgggagttgc tatttaatta cgttacgccg
    32581 gagctgacat gaaaaccaaa cttaacgaac tgcttgaatt ccctactcct tttacttaca
    32641 aagttatggg gcaggcgtta cctgagctgg ttgatcaggt ggttgaagtg gtacagcgcc
    32701 atgcgccagg tgactacacc ccaacggtaa aaccaagcag caaaggcaac taccactcgg
    32761 tatctatcac tatcaacgcc actcatatcg agcaggttga aacactgtat gaagaactgg
    32821 gcaaaatcga tattgtccgc atggttctgt aactcgcttc tccgttaccc ggctcctggt
    32881 cgggtaactc ccctctcctc gctgtgatat acttttcccc cacttttact cattctccac
    32941 ggagatgccg ttttgtatca ggataaaatt cttgtccgcc agctcggtct tcagccttac
    33001 gagccaatct cccgggctat gcatgaattc accgataccc gcgataatag tacccttgat
    33061 gaaatctggc tggtcgagca ctatccggta ttcacccaag gtcaggcagg aaaagcggag
    33121 cacattttaa tgccgggtga tattccggtg atccagagcg atcgcggtgg gcaggtgact
    33181 tatcacgggc cgggacaaca ggtgatgtat gtgttgctta acctgaaacg ccgtaaactc
    33241 ggtgtgcgtg aactggtgac cttgcttgag caaacagtgg tgaataccct ggctgaactg
    33301 ggtatagaag cgcatcctcg ggctgacgcg cctggtgtct atgtcgggga aaagaaaatt
    33361 tgctcactgg gtttacgaat tcgacgcggt tgttcattcc acggtctggc attaaacgtc
    33421 aatatggatc tttcaccatt tttacgtatt aatccttgtg ggtatgccgg aatggaaatg
    33481 gctaaaatat cacaatggaa acccgaagcg acgactaata atattgctcc acgtttactg
    33541 gaaaatattt tagcgctact aaacaatccg gacttcgaat atattaccgc ttaattccat
    33601 acatcaatgg cccaatttac actgggtcat tactcataat acctgctaat gctttacatc
    33661 tccttcgcgt ttgtatattt tctttatcac ttgcagaaat gcggtgccac gccttgctcc
    33721 gtaactcacc ccattgatta acctattgaa atagttgatt atggtgagca aaagcgttcc
    33781 gttccaatta gacataagat tattgtaatt ttttaaatat tatttttcca tgaataaaat
    33841 tggagtaagt gcgtggatag taataatcaa attgaaccct gtttaagcag aaagtcgtca
    33901 gaaggtaaac cacaaatatt tacaacctta cgaaatattg atcttaacct tctgactatt
    33961 tttgaagctg tatatgtaca taaagggatc gttaatgcag cgaaagtgct taatctgacc
    34021 ccctcggcaa tcagtcagtc tattcagaaa ctgcgcgtta tattccctga cccattgttt
    34081 attcgcaaag gccagggtgt cactcctacc gcatttgcga tgcatctaca tgagtatatc
    34141 agtcagggcc ttgagtccat tcttggcgcg ctggatatcg aaggaagcta tgataagcaa
    34201 cgaacgataa ctattgcgac cactccctcg gtcggagccc tggtccttcc tgtcatctat
    34261 cgggcgatta aaactcacta tccgcagctt ttactgcgca acccgcccat cagcgacgcg
    34321 gaaaaccaac tcagtcagtt tcaaaccgat ctcatcatcg ataacatgtt ttgcaccaat
    34381 cgtacggtgc aacatcatgt tctgttcacc gacaatatgg tgttaatttg ccgcaaggga
    34441 aatccactac tctctttaga agatgacaga gagactatcg acaacgctgc gcatgtactt
    34501 ctgttaccgg aagggcaaaa tttcagcggt ctgcggcaga gagttcaaga gatgtttccg
    34561 gaccggcaaa tcaatttcac cagctacaac attttgacaa tcgctgcact ggttgccaac
    34621 agtgacatgt tagcgattat tcccagccgt ttttataacc tgtttagtcg ttgctggccg
    34681 ctggaaaaac tgccttttcc gtccttaaat gaggagcaaa tagatttctc tatccactac
    34741 aacaaattca tcctgcgtga tcccatcctg cacggagtaa tagatgttat ccgtaatgca
    34801 ttttgaaaaa gtattgttac agccagatca cagcgcagaa cgataaaatg gcacaatcca
    34861 acaacaattt tacattttcg cgaccgcttt ggctgctttc aggtccgttt caatgatata
    34921 ctgccagtcg ttaattcaaa aatagttgat aattacaaca atctattgaa ttgaaacgct
    34981 ttccttcgta attcgcaact ggaacacgca cgctatgagt aaacccattg tgatggaacg
    35041 cggtgttaaa taccgcgatg ccgataagat ggcccttatc ccggttaaaa acgtggcaac
    35101 agagcgcgaa gccctgctgc gcaagccgga atggatgaaa atcaagcttc cagcggactc
    35161 tacacgtatc cagggcatca aagccgcaat gcgcaaaaat ggcctgcatt ctgtctgcga
    35221 ggaagcctcc tgccctaacc tggcggaatg cttcaaccac ggcacagcaa cgtttatgat
    35281 cctcggcgct atttgtaccc gccgttgtcc gttctgtgat gttgcccacg gtcgcccggt
    35341 agctcctgat gccaatgaac cagtgaaact ggcgcagacc attgccgata tggcgctgcg
    35401 ttatgtggtt atcacctccg ttgaccgtga tgacctgcgc gatggcggtg cccaacactt
    35461 tgcggattgt attactgcca ttcgggaaaa aagcccgcaa atcaaaattg aaactctggt
    35521 gccggatttc cgcggtcgaa tggatcgtgc tctggatatt ctgactgcaa cgcccccaga
    35581 tgtgttcaac cataacctgg aaaacgtacc gcgtatttac cggcaggtac ggcctggcgc
    35641 agattacaac tggtcgctga agctgctgga acgctttaaa gaagcgcatc cggaaatccc
    35701 gaccaagtct ggtctgatgg tgggtctggg tgaaaccaat gaagaaatta ttgaggtaat
    35761 gcgcgacctg cgccgtcatg gtgtgacgat gttaacgctg gggcaatatt tgcagccaag
    35821 ccgccatcac ctgccggttc aacgttacgt tagcccggat gagttcgacg aaatgaaagc
    35881 cgaagcgctg gcgatgggct ttacccatgc tgcatgcggt ccgtttgtcc gctcttctta
    35941 ccacgccgat ttgcaggcga aaggaatgga agttaagtaa tcctgtaata attacttaca
    36001 tctgtctgat aaaaaacccg ctttttgaag cgggtttttt gtatcgaaca agatattgag
    36061 ggagcgtcct gctcgccacg tcactcttta tgagagagct tttcagcctg aagatcaacg
    36121 tctgcgcctt ttttcgccgc agcatcgtca tcattcatcg ccttcttgaa ccctttaatg
    36181 gccgctccaa ggtctccgcc cagcgtacgt aacttcttag tcccaaacag cagaacgacc
    36241 agcgccgcaa ctaccagcag tttggtaata ctaatctcac ccatagatac cttcttgaca
    36301 tataaccggc aacagaacgc gccttaaaaa ccagagaata ttaacggtca ttcgacgcgc
    36361 gcacacaata gcaatctgta acaaggtgaa tcaagcgccg agtaaaaaaa catcataata
    36421 attgcggcgg cgcaaagcga cggttgttta agatgggcag ttgcgcgcgc acctgacgca
    36481 cacgttcggg cgtcacttcc gccataatga gtgcaggcat ttctgacgct gccgcaatgg
    36541 tgacgccaaa gggatctata atccggcttt gcccgatatt tttgttcccg cactcccccg
    36601 ccgccaccat ataacaggtg gtatccagcg cacgagcggc aagcaacgtt gaccagtgat
    36661 gctctttgag cggcccacga acccaggcgg caggaagtac caggatttca gctccctgta
    36721 aagcctgtgc cagcgccagc tctggaaagc gtaagtcata acaggtcatc agaccgacct
    36781 tcatcccttc cacctccagt aacggagcta tttcattacc agcatcaaca tggcgtgatt
    36841 cctgaatggc aaatgcatca tagagatgca gtttggcata acgggcgacg atgtttcctg
    36901 cctgaagtgc caccagcata ttccatgcgc gccccggcgt tgaaggaaga tgaatcgtca
    36961 gaattgtcgt catcatgtta cgtttacttt ctcgccgtaa acgtccgagg aattcgcctt
    37021 ccagcagctg tgctgattta accgatagat ctgcatcatg atcatcgcgc gccagcaatg
    37081 cttccggcag gacaaacagc gatacgtcgt tttccgccgc ctgcgccatc aacgaggcac
    37141 aaatctcagc gttcttttcc cacacagatg taacagcaaa ctgtcctgca gcaaccagca
    37201 ttgccacctc ctgtcagagt cgtaaaattc gccaacgacg attattcggc gttacactta
    37261 tcactcatac aaatcaaata gcaggatttt gcagtgttac aacttctttt agcagttttt
    37321 attggcggtg gtacgggaag cgtagcgaga tggctgttaa gtatgcgatt taacccgctg
    37381 catcaggcga ttccgttggg gacgctggca gcaaacctga ttggggcatt catcatagga
    37441 atgggattcg cctggttcag caggatgacg aacattgatc cagtgtggaa agtattaatc
    37501 accaccggat tttgtggcgg tctaacaacc ttctcaacat tttcggcaga agtggtgttt
    37561 ttgttacaag agggccgctt tggctgggca ttactgaacg ttttcgtcaa ccttctgggg
    37621 tcttttgcca tgaccgcact ggcattctgg ctgttttcgg cctcaaccgt acactaaaga
    37681 ccaaaaaaaa cccgctgatt aagcgggttt tgaattcttg ctgacgtatc ttacagagcg
    37741 attacgtttg cagcagaagg gcctttggca ccgttagtga tttcgaactc tacgcgctga
    37801 ccttcagcaa gagttttaaa accattagtc tggattgcag agaagtgtac gaacacgtct
    37861 ttgctgccgt cttccggagt aatgaaaccg aatcctttgg actcattaaa ccacttaacg
    37921 ttacctttaa tcttagacat caaaattacc tttacatgaa aaatagacac aaatgctgtg
    37981 tcggttatca gtacaccaat tgtggtacgc tttgtccagt gcaactttac taaaaagtga
    38041 taaaagtcgc gttatttttg atgttgctta ttttagttat tgattttaaa gaagttacta
    38101 aaacttcatt tgtctcaaaa ctgaaagcgc atccaggcaa agtacacatt gccattgttg
    38161 taggtacccg gaatgtaggt catctgaaaa gtcactgggc cataacccac ggaggccaat
    38221 ggcagtagaa ccgggagagg gatgtaattc cagttatcgc gtgccgttac gccagcggtg
    38281 aatcccagac ctaaatgaaa attttcatcc gccagcggtc gccaggtact ttcccatccg
    38341 tatccggcaa tcggttccca tttgttccac gagtccttaa atgccatggc atacaggcca
    38401 tgccagtttc ctttttcatc ccaacgcgac aggccaaaac cgccacccca cggtcgctcg
    38461 ttatagcgat cggttttttc tttgtcgtaa gcgaaacgtg catgccaggt gatggcagga
    38521 atatataaat cataatgttc aggctgttgc caggtttgtg caatattttc tctaaacgtt
    38581 gtcatcctct catctgcgtt agcaaaaact ttaccaacgc tgattaactg aataaaaaca
    38641 aaggaaaaga tagcgacata tttactcacg ttcatttgtg accataaaac atttatcaaa
    38701 aatctactac tagcatagca aagctacaat taacataacc ttaatagacc caacataaag
    38761 aataatctga atagctgctc tatttatttc cgcttgttca agttaaatat ttccgcgcat
    38821 cgattaaaga tgatgcgtcg ttttctgcga tgggaatagt caaaaaagaa aaaccaagtc
    38881 ttttttgatg acaaaatgca atcaaggaaa aattatttta tttttttaac gagatacaac
    38941 aatcatctta acgaagtata taatatatat cgtcaaccca tgctcatatt agattatttt
    39001 ttgagcgcac agccgcagct aacaccattg caattaacaa atttgcatca atccaccatc
    39061 aatttacaca cattattatg tgataattgc caaccgctaa atatgcgttt tgttatctat
    39121 gtataaaaac agcaacttca atgtcttaat ggcagttttt cttgatttta atcagcattc
    39181 atcgccaatt tattgggcat attttttcct taagctttag gaagttttta ttatttactt
    39241 tgggcctgga gacaggaaaa attatgctga cattcattga actccttatt ggggttgtgg
    39301 ttattgtggg tgtggctcgc tacatcatta aagggtattc cgccactggt gtgttatttg
    39361 tcggtggcct gttattgctg attatcagtg ccattatggg gcacaaagtg ttaccgtcca
    39421 gccaggcttc aacaggctac agcgccacgg atatcgttga atacgttaaa atattactaa
    39481 tgagccgcgg cggcgacctc ggcatgatga ttatgatgct gtgtggcttt gccgcttaca
    39541 tgacccatat cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca ttgcagtata
    39601 ttaactcccc ttacctgctg atgattgccg cctattttgt tgcttgtctg atgtcactgg
    39661 ccgtctcttc cgcaaccggt ctgggggttt tgctgatggc aaccctgttt ccggtgatgg
    39721 taaacgttgg tatcagtcgt ggcgcagcag ctgccatttg tgcctccccg gcggcgatta
    39781 ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca ggcttccgaa atgtcgctga
    39841 ttgacttcgc cttcaaaacg acgctgccta tctcaattgc tgcaattatc ggcatggcga
    39901 tcgcccactt cttctggcaa cgttatctgg ataaaaaaga gcacatctct catgaaatgt
    39961 tagatgtcag tgaaatcacc accactgccc ccgcgtttta tgccattttg ccgttcacgc
    40021 cgatcatcgg tgtactgatt tttgacggta aatggggtcc gcaattacac atcatcacta
    40081 ttctggtgat ttgtatgctg attgcctcca ttctggagtt cctccgcagc tttaataccc
    40141 agaaagtttt ctctggtctg gaagtggctt atcgcgggat ggcagatgcg tttgctaacg
    40201 tggtgatgct gctggttgcc gctggggtat tcgctcaggg gcttagcacc atcggcttta
    40261 ttcaaagtct gatttctatc gctacctcgt ttggttcggc gagtatcatc ctgatgctgg
    40321 tattggtgat tctgaccatg ctggcggcag tcacgaccgg ttcaggcaat gcgccgtttt
    40381 atgcgtttgt tgagatgatc ccgaaactgg cgcactcttc cggcattaac ccggcgtatt
    40441 tgactatccc gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc gtttctggcg
    40501 tagtcgttgc ggttgccggg atggcgaaaa tctcaccatt tgaagtcgta aaacgcacct
    40561 cggtgccggt gcttgttggg ctagtgattg ttatcgttgc tacagagctg atggtgccag
    40621 gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt ttcctccggc
    40681 tttatcatgt cacccactgt ggtatatgcg ttgtggtctg ccaactttgc cgtgaacaat
    40741 ttcagcaata atcagatggc ggctggcgca atattcaaga taacgcctgg cagtggtgcg
    40801 gctgatggtc agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag gctctttaaa
    40861 cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg gtcggcagtt cgtctttggg
    40921 ttcaccgcga gcataggcat taaacatctc atcaatttgc ttctggctgg cgctatcaat
    40981 actttccagc atatgtttac gctggcggaa acgggttagc gtttgcccca gtgtattgat
    41041 tcacttgaag tacgaaaaaa ccgggagaac attggattat tcgggatctg atgggattag
    41101 atttggtggg gcttgcaagc ctgtagtaca aattttaggc gttaaccaat gaaacgcgaa
    41161 agatagtaaa aaattgcttt tgtttcattg aaaaaacgaa aaacaaaaac actataaatc
    41221 atttcaatca tagcttcaaa aaacgttcaa aaccgataac aaccaggctg tcaccaaatg
    41281 actcatatca caaatcagct catgccgttt aggtatgtta catgtgtgat tatgtgaggt
    41341 gaagtatgtt ttagctggct catggttgtt atacggcttt tttcgcatct tgtggttcca
    41401 acaaaggagc tacaggctct tcctgtttat gaacaccata cattgacaaa atctcttggc
    41461 gagtttctgg aggtaattta tgcagcgcca aagctgtttg tagtgtttgg cgatcaatac
    41521 ccagcgttgc caatagggtg tcaacggaga tggaggggcg ttcttgcatt aatccggaaa
    41581 gcccatattt agccatgttt agctctatct ctcggacttc attttctcct agtttattga
    41641 ttaacaggga gaacattttc aatgtatcag cattcatttc ttcgatagca attttttgca
    41701 tagccggtat tgccgggata tattcaaccc caggcccgcg acgcccttct tgtgaacgct
    41761 tcggccatcc ttctttcttt gctcgtttat gcaccccctg tattgtccca ggcattccat
    41821 ccagttcagt aatttgtttg ggtgtcatcc actggtttat ctcatttgcg cttttcataa
    41881 actaagttta ccagatttat ctatataaaa atcaatggat tacaaaataa accacattaa
    41941 ctgatataaa ccggtttggt ttatttggta atgtataaaa cagataatca agacgaatta
    42001 tttgttttgg taacccttta tcatacgcaa tatggttggt gtgcgaaaga tgagtaacga
    42061 ttggcatcgg gccgacgtga ttgcggccct caaaaaaaca ggaacccctc tggcaaaatt
    42121 gtccagacgt tcaggttatt caaccgggac gcttgcaaat gcattagtca ggccatggcc
    42181 taaaggtgaa cgcattattg ctgacgcctt aaacatgaaa cctgaggaaa tttggcctag
    42241 tagatatcta agggatcaca atgagagagt ggatatcagc aattgaatgc atgggtttat
    42301 ctggtttacc tggttcatta atgggggttc acaaaagagc tcagagagaa ggatggctca
    42361 agagaagaag agaaggaatt agaggggttg cttacgaata ccatatatct tctcttccta
    42421 acattgctcg agctgaagtt ttgctccaac aaggaaaatt taaaaccagt cagggatgtt
    42481 ttgaaatcgc ccgccccacg ctggaagctc atgattatga tcgtgaggca ctatggagca
    42541 aatgggataa agccagcgat tcccagcgca gcctggctga aaaatggttg ccctcaattc
    42601 aggccacaga cgaaatgctg aaccagggga tttcaacgaa aacggctttt gcgaccgttg
    42661 cagggcatta ccaggtcagc gcatccactt tgcgggacaa gtattaccag gtacagaagt
    42721 ttgcgaagcc tgactgggcg gctgcacttg ttgatggacg tggagcatcc cgtcgcaatg
    42781 ttcacaaaag tgaatttgac gaggatgcct ggcagtttct gattgcagat tatctgcgac
    42841 cggaaaaacc cgccttccgc aaatgttatg agcgtctgga actggcagcc cgcgagcatg
    42901 gctggagtat tccctcccgt gccacggcct ttcgccggat tcagcaactg gacgaggcaa
    42961 tggttgttgc ctgtcgtgaa ggtgaacatg cactgatgca tctgataccg gcacagcagc
    43021 gaactgtgga acacctggac gccatgcagt ggatcaacgg cgacggttat ctgcataacg
    43081 tctttgtacg ctggtttaac ggtgatgtga tccgtccgaa aacatggttc tggcaggatg
    43141 tgaaaacccg aaaaattctg ggctggcgct gcgatgtgag cgagaacatt gattcaattc
    43201 gcctctcgtt catggatgtt gtgactcgct acagtatccc ggaggatttt cacatcacca
    43261 ttgataacac ccgtggtgct gcgaataaat ggctgacggg aggcgcgccc aatcgctacc
    43321 gctttaaggt aaaagaggac gatccaaaag gactgttttt actgatgggt gcgaaaatgc
    43381 actggacaag cgttgttgcc ggtaaaggct ggggccaggc aaaacctgtt gaacgtgctt
    43441 tcggtgttgg tgggcttgag gaatacgttg ataagcatcc ggcactggct ggcgcatata
    43501 cggggccaaa tccgcaggca aaacctgata actatggcga ccacgctgtt gatgcagagc
    43561 tgtttctgaa aacctttgcc gaaggtgtgg cgatgttcaa tgccagaaca ggccgtgaaa
    43621 cagaaatgtg cgggggcaaa ctctcgtttg atgatgtttt cgagcgtgaa tacgccagaa
    43681 cgattgtgcg taagccaacc gaagaacaaa aacggatgct gttactgcct gccgaggcgg
    43741 tgaacgtttc acgcaaaggc gagtttacgc ttaaagttgg cggctccctt aaaggcgcga
    43801 aaaacgttta ttacaacatg gcattaatga atgccggagt gaaaaaagtt gtggtcagat
    43861 ttgatccaca gcagttacac agcacggttt attgag
//
LOCUS       DADLTY010000007        42980 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.007, whole genome shotgun sequence.
ACCESSION   DADLTY010000007 DADLTY010000000
VERSION     DADLTY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.007"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(7..486)
                     /locus_tag="JN334_000344"
     CDS             complement(7..486)
                     /locus_tag="JN334_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HAY7436615.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            complement(670..2088)
                     /locus_tag="JN334_000345"
     CDS             complement(670..2088)
                     /locus_tag="JN334_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7436616.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(2101..6561)
                     /gene="gltB"
                     /locus_tag="JN334_000346"
     CDS             complement(2101..6561)
                     /gene="gltB"
                     /locus_tag="JN334_000346"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAY7436617.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            7236..8165
                     /locus_tag="JN334_000347"
     CDS             7236..8165
                     /locus_tag="JN334_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAY7436618.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            8261..10597
                     /gene="arcB"
                     /locus_tag="JN334_000348"
     CDS             8261..10597
                     /gene="arcB"
                     /locus_tag="JN334_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HAY7436619.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            10827..11480
                     /gene="elbB"
                     /locus_tag="JN334_000349"
     CDS             10827..11480
                     /gene="elbB"
                     /locus_tag="JN334_000349"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HAY7436620.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            11477..12204
                     /gene="mtgA"
                     /locus_tag="JN334_000350"
                     /pseudo
     CDS             11477..12204
                     /gene="mtgA"
                     /locus_tag="JN334_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(12201..12833)
                     /gene="yrbL"
                     /locus_tag="JN334_000351"
     CDS             complement(12201..12833)
                     /gene="yrbL"
                     /locus_tag="JN334_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HAY7436621.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(13046..13318)
                     /gene="npr"
                     /locus_tag="JN334_000352"
     CDS             complement(13046..13318)
                     /gene="npr"
                     /locus_tag="JN334_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HAY7436622.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            complement(13315..14169)
                     /gene="rapZ"
                     /locus_tag="JN334_000353"
     CDS             complement(13315..14169)
                     /gene="rapZ"
                     /locus_tag="JN334_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAY7436623.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            complement(14215..14706)
                     /gene="ptsN"
                     /locus_tag="JN334_000354"
     CDS             complement(14215..14706)
                     /gene="ptsN"
                     /locus_tag="JN334_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HAY7436624.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            complement(14824..15111)
                     /gene="hpf"
                     /locus_tag="JN334_000355"
     CDS             complement(14824..15111)
                     /gene="hpf"
                     /locus_tag="JN334_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAY7436625.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            complement(15134..16567)
                     /gene="rpoN"
                     /locus_tag="JN334_000356"
     CDS             complement(15134..16567)
                     /gene="rpoN"
                     /locus_tag="JN334_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAY7436626.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            complement(16615..17340)
                     /gene="lptB"
                     /locus_tag="JN334_000357"
     CDS             complement(16615..17340)
                     /gene="lptB"
                     /locus_tag="JN334_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAY7436627.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            complement(17347..17904)
                     /gene="lptA"
                     /locus_tag="JN334_000358"
     CDS             complement(17347..17904)
                     /gene="lptA"
                     /locus_tag="JN334_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HAY7436628.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            complement(17873..18448)
                     /gene="lptC"
                     /locus_tag="JN334_000359"
     CDS             complement(17873..18448)
                     /gene="lptC"
                     /locus_tag="JN334_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAY7436629.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            complement(18445..19011)
                     /gene="kdsC"
                     /locus_tag="JN334_000360"
     CDS             complement(18445..19011)
                     /gene="kdsC"
                     /locus_tag="JN334_000360"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAY7436630.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            complement(19032..20018)
                     /gene="kdsD"
                     /locus_tag="JN334_000361"
     CDS             complement(19032..20018)
                     /gene="kdsD"
                     /locus_tag="JN334_000361"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HAY7436631.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            complement(20032..20997)
                     /locus_tag="JN334_000362"
     CDS             complement(20032..20997)
                     /locus_tag="JN334_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAY7436632.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            21207..22016
                     /gene="mlaF"
                     /locus_tag="JN334_000363"
     CDS             21207..22016
                     /gene="mlaF"
                     /locus_tag="JN334_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HAY7436633.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            22024..22806
                     /gene="mlaE"
                     /locus_tag="JN334_000364"
     CDS             22024..22806
                     /gene="mlaE"
                     /locus_tag="JN334_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAY7436634.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            22811..23362
                     /gene="mlaD"
                     /locus_tag="JN334_000365"
     CDS             22811..23362
                     /gene="mlaD"
                     /locus_tag="JN334_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAY7436635.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            23381..24016
                     /gene="mlaC"
                     /locus_tag="JN334_000366"
     CDS             23381..24016
                     /gene="mlaC"
                     /locus_tag="JN334_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HAY7436636.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            24016..24309
                     /gene="mlaB"
                     /locus_tag="JN334_000367"
     CDS             24016..24309
                     /gene="mlaB"
                     /locus_tag="JN334_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HAY7436637.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            24469..24723
                     /gene="ibaG"
                     /locus_tag="JN334_000368"
     CDS             24469..24723
                     /gene="ibaG"
                     /locus_tag="JN334_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HAY7436638.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            24778..26037
                     /gene="murA"
                     /locus_tag="JN334_000369"
     CDS             24778..26037
                     /gene="murA"
                     /locus_tag="JN334_000369"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAY7436639.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(26085..26363)
                     /gene="sfsB"
                     /locus_tag="JN334_000370"
     CDS             complement(26085..26363)
                     /gene="sfsB"
                     /locus_tag="JN334_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HAY7436640.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(26590..27561)
                     /gene="ispB"
                     /locus_tag="JN334_000371"
     CDS             complement(26590..27561)
                     /gene="ispB"
                     /locus_tag="JN334_000371"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAY7436641.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            27819..28130
                     /gene="rplU"
                     /locus_tag="JN334_000372"
     CDS             27819..28130
                     /gene="rplU"
                     /locus_tag="JN334_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAY7436642.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            28151..28408
                     /gene="rpmA"
                     /locus_tag="JN334_000373"
     CDS             28151..28408
                     /gene="rpmA"
                     /locus_tag="JN334_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAY7436643.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            28535..29500
                     /locus_tag="JN334_000374"
     CDS             28535..29500
                     /locus_tag="JN334_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7436644.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            29516..30688
                     /gene="cgtA"
                     /locus_tag="JN334_000375"
     CDS             29516..30688
                     /gene="cgtA"
                     /locus_tag="JN334_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HAY7436645.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(30874..32307)
                     /gene="dacB"
                     /locus_tag="JN334_000376"
     CDS             complement(30874..32307)
                     /gene="dacB"
                     /locus_tag="JN334_000376"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY7436646.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            32555..33031
                     /gene="greA"
                     /locus_tag="JN334_000377"
     CDS             32555..33031
                     /gene="greA"
                     /locus_tag="JN334_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAY7436647.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            complement(33187..33480)
                     /gene="yhbY"
                     /locus_tag="JN334_000378"
     CDS             complement(33187..33480)
                     /gene="yhbY"
                     /locus_tag="JN334_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAY7436648.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            33606..34235
                     /gene="rlmE"
                     /locus_tag="JN334_000379"
     CDS             33606..34235
                     /gene="rlmE"
                     /locus_tag="JN334_000379"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAY7436649.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            34335..36269
                     /gene="ftsH"
                     /locus_tag="JN334_000380"
     CDS             34335..36269
                     /gene="ftsH"
                     /locus_tag="JN334_000380"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAY7436650.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            36359..37207
                     /gene="folP"
                     /locus_tag="JN334_000381"
     CDS             36359..37207
                     /gene="folP"
                     /locus_tag="JN334_000381"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAY7436651.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            37200..38537
                     /gene="glmM"
                     /locus_tag="JN334_000382"
     CDS             37200..38537
                     /gene="glmM"
                     /locus_tag="JN334_000382"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAY7436652.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            38765..39097
                     /gene="secG"
                     /locus_tag="JN334_000383"
     CDS             38765..39097
                     /gene="secG"
                     /locus_tag="JN334_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAY7436653.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            39112..39198
                     /locus_tag="JN334_000384"
     tRNA            39112..39198
                     /locus_tag="JN334_000384"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39146..39148,aa:Leu,seq:gag)
     gene            39676..41283
                     /locus_tag="JN334_000385"
                     /pseudo
     CDS             39676..41283
                     /locus_tag="JN334_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(41291..42634)
                     /gene="argG"
                     /locus_tag="JN334_000386"
     CDS             complement(41291..42634)
                     /gene="argG"
                     /locus_tag="JN334_000386"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAY7436654.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
BASE COUNT        10178 a        10587 c        11512 g        10703 t
ORIGIN      
        1 cttcgtttat ttgcatgaat atacaaaccg aaaataggag atgcaataca aaagctgtgg
       61 aaatatcaaa cagcctctca atttggcaga cagcgtctgc caaatctttg ccgatgggaa
      121 ttctgcagat ggtgtctgcg gaattcccaa tggtgttaat tcgccagaca ctgtctggcg
      181 aatttcaaca tgttgaaaaa agaggtaaaa aagcctcatt tggcgtaagt tttttgcaga
      241 gaccccacgt ttataccgtc gacttaaatc cttcgatagc cgatcaatca actgtgcacc
      301 ataagctgct ctggcctcac caccttgttc aaattcgaca atccgtcggc caatttccca
      361 gtacgtcgcg gtcatcagcg cgttaacgct acgtaccgtt tccgttcggg cgctatcgac
      421 cagatgaata atgccgttgt ggatttgctg gtagcctgtt gaggttctct ctgagagcga
      481 ttccattatc tgtcccttta tatggcgctg cgttgaataa tggaatcagc tatacgccgg
      541 gcatctaccg gtaacaatcc agatgagcgt attacattgg ttttcttcag attatgcgag
      601 gcgatttcca gaaagaaagg agaatttcag cgccgactct tttctgagga ggcggcgctg
      661 ggacagagct taaacttcca gccagttcat aatgccgtct gccgctttgc gaccttcggc
      721 gatggcggta acgaccagat cggaaccacg gacgatatcg ccgccagcaa agattttcgg
      781 gttgctggtc tggaaagcgt tgtcgttgcc ttccggagcg atgatgcggc cttgtgaatc
      841 cagctcgacg ctgtgttttg ccagccattc catgttgtgt ggacggaaac caaacgccat
      901 gatcaccgca tctgccggaa cgatatgttc ggaacctgca acgatctccg cgcggcgacg
      961 gcctttggca tccggttcgc ccatttcggt acgtaccatt tttacgccgc tgactttgcc
     1021 gttaccgttc acttcaatcc ccagtggctg gacgttgaat ttgaactcta cgccttcttc
     1081 ccgcgcgttt ttcacttcgc ggcgggaacc cggcatgttc tcttcatcac gacgataggc
     1141 acaggtaacg tgctttgccc cctggcgtac agacgtgcgc acgcagtcca tcgcagtgtc
     1201 gccaccgcca agtactacca cgcgtttgcc ttccatgctg acgaacggtt cgtcgcgggt
     1261 ttcaccaaag cccattaact gtttggtgtt ggcgataagg aacggcagcg ctgcgtacac
     1321 gccatcggcg tcttcgtttt ccagcccgcc gcgcattgac tgataagtcc caacgccaag
     1381 gaacacggca tcgtaatcac tcagcagatc gtccagctgt acgtcgcggc ccacttcggt
     1441 attgagtttg aattcaatac ccatgccggt gaagatttca cggcgacgcg tcattacctc
     1501 tttttccagc ttgaaggccg gaataccgaa ggtcagcagc ccgccaattt ctggatgacg
     1561 gtcgaagaca acggctttta cgccgttacg cgtcaggaca tccgcacacg ccagacctgc
     1621 cgggcctgca ccgataatcg ccactttttt accggtttgc ttcacgccgg acatatccgg
     1681 acgccagccc atttcgaacg ctttatcatt gatataacgc tcaatgttgc cgatggtcac
     1741 cgcaccgaac tcatcgttca gggtgcagga accttcgcac agacggtctt gcgggcagac
     1801 acgtccgcat acttccggca gtgtgttggt ctgatgcgac agttctgccg cttcaaaaat
     1861 acgcccctcg ttggcgagct tcagccagtt tgggatgtag ttgtgtaccg ggcatttcca
     1921 ctcgcagtat gggttgccgc acgacagaca gcggtcagcc tgcgctttgg cctggccttc
     1981 ggaaaacggc tcgtaaattt caacaaactc aattttacgg atcttcagcg gtttctttgg
     2041 cggatcaacg cgctgcaggt cgataaattg ataaacattc tgactcattg ttgctacccc
     2101 ttactgcgcc tgcacgcgca actctgctgc gctacgacta cggtgaccca gcagtgcttt
     2161 tacatcactg gacttcggtt taaccagcgc gaatttcgtg gcgaaggttg accagttcgc
     2221 cagaatctct tcaccgcgct gagaaccggt atgctgaaca tgctcggtga taagaccgcg
     2281 cagatgctct tcatggatcg ccagatcgtc gacgcttaag acttcgacca gttccgggtt
     2341 aacgcgtttg cggaaatcac cgctttcatc gagaacgtaa gcgaagccgc cggtcatgcc
     2401 cgcgccgaag ttaacgccgg ttttacccag aatacagaca ataccgcccg tcatatattc
     2461 acagccgttg tcgccaatgc cttctaccac ggtgatagca ccggagttcc gcacgccgaa
     2521 acgttcaccc gcgcggcctg cggcatacag acgaccaccg gtcgcgccat acaggcaggt
     2581 gttgccgata atgcttgctt catggctgcg gaaggcggaa ccaaccggag gacgaatggc
     2641 gattaagccg cccgccatgc ctttaccgac atagtcgttg gcatccccgg tcaggtacag
     2701 ttccacgccg cccgcgttcc acacaccgaa actctggcct gcggtgccgt tgaagtacgc
     2761 tttgatagga tcggctgcca gcccctgatc gccgtgcgtc tgggcgatat agcctgaaag
     2821 cgacgcgccg acagaacggt cggtgttgcg gatatcgaac cagaaggttt tgctctggcg
     2881 ttcatcgaca aacggtttcg cctgttgcag caactgcgcg ttcagtaggc cgttatcaaa
     2941 cggcgggttg ttttcagtgc agtagagtga cttacctgca tgtggctcgg cagtctccag
     3001 cagcttcgac agcgccagtt tctgctgttt ggcggtgaaa ccgtccagct ctttcagcag
     3061 gtcggtgcga ccaatcagat ccaccagacg cgttactccc agctgtgcca tcagctcgcg
     3121 ggtttcacgg gcgataaact caaagtaatt agtcaccttg aacggcaggc cgtgatagtg
     3181 attcttacgc agtttgtcat cctgagttgc tacacccgtg gcgcagttgt tcaggtggca
     3241 aatacgcaga tatttacagc cgagcgccac cattgggcca gtgccgaagc cgaagctttc
     3301 tgcgccgaga atcgccgctt tgatgatatc gacacccgtt ttcaggccgc catcaacctg
     3361 caaacgaatt ttatgacgca agccgttggc aaccagcgcc tgttgggttt ccaccagacc
     3421 gagttcccac gggcagcctg catatttcac tgacgacagc gggcttgcgc cggtgccgcc
     3481 gtcatagccc gcgatggtga tcaagtccgc ataagctttc gccacgccag tcgcgatggt
     3541 gccaacgcct ggttcggaca ccagcttaac ggagatcatc gctttcgggt taacctgctt
     3601 gaggtcgaaa ataagctgcg ctaagtcttc gatagagtag atatcgtggt gcggcggcgg
     3661 ggagatcagc gtcactccgg gcaccgaata gcgcagtttg gcgatgtaag gagtgacttt
     3721 gtcgcccggc aactgaccac cttcgcccgg cttcgcgccc tgggcgactt taatctgaat
     3781 gacgtcggca ttgaccagat acgccggagt aacgccgaag cgaccagaag ccacctgctt
     3841 gatgcgcgac actttgttag tgccatagcg cgccgggtct tcgccgcctt caccggagtt
     3901 cgagttaccg ccgatgctat tcatcgcttc cgccagcgcc tcgtgggctt ccgggcttaa
     3961 cgcgccgata gacatcgcgg cggtatcaaa gcgtttaaac agttcgcttg ccggttcaac
     4021 atcagcaatg ttgaccgcgt tttcacccgg cgtaattgcc agcagatcgc gcagcgtggt
     4081 tgccggacgc tcattaacca gcttcgcgta ttcctgatag tcgctgtact cgccgctttg
     4141 taccgcctgt tgcagcgtgc gcaccacgtc cgggttgtag gcgtggtatt cgccgccgtg
     4201 gacgtatttc agcaggccgc cctggctaat cggcttgcgc gccagccagg cacgtttcga
     4261 cagattcagc agatcctgct ggaagtcttc aaagcttgcc ccaccaatgc ggctgaccgc
     4321 gccctggaag cacaggccca ctacatcatc gtgtagaccg accgcttcaa acagtttcga
     4381 gcagcggtaa gaggcgatgg tggagatgcc cattttggac atgattttgt acaagccttt
     4441 gttgatgccg ttacggtagt tgagcatcac ggtacgataa tctttggcaa tcgcgtgggt
     4501 gtctaccagg cggcccagcg tttcataggc aaggtacgga taaatagccg tcgcgccgaa
     4561 acccagcaac acggcgaagt ggtgcggatc gcgggcgctg gcggtttcga cgatgatgtt
     4621 ggcatcgcaa cgcaggcttt gatcgaccag acgggtctgg atcgcgccaa ccgccatcgg
     4681 agccggaacc ggcaggcgat ctttagcgat attccggtcg gagagcacca gcaacacggt
     4741 gccgctacgt accatttttt cggctttgtc gcatagctct ttgactgtcg cttcgagcgt
     4801 ggttttagtg acgtcaaagg tgatatccag cgtatctgcg cggtagtgct cctctttcat
     4861 cgtcgtgagc tgtttgaaat cggagtagag cagaatcggc gatttaaagc ttaaacggtg
     4921 cgcctggccc tctgcttcgc aaaagacgtt catttcacga ccgatactgg tggcgagcga
     4981 cataacatgc gcttcacgca gcgggtcgat tggcgggtta gtcacctggg caaactgctg
     5041 gcggaagtag tcgtaaataa tgcgcggctg actggagagc acggcgaatg gggtatcatc
     5101 gcccatcgaa ccgaccgctt cctgaccgtt ttcgcccagt acgcgaatta cggagtccag
     5161 ctcttccgcg ctgtagttaa actgtttctg gtagctggca agcgtgtcgt cgtccagttc
     5221 gcggctaccc acttcttcat cgggcagatc ttcaaacggt accagtcggc ggacgttttt
     5281 ctccatccac tctttataag ggtggcggct tttcagatcg tcatcggttt ctgccgagtg
     5341 cagaatacgc ccactgcgag tgtcgatcac catcagttcg cctggcccga cgcggccttt
     5401 ttcgaccact tcgtcaggct ggtaatccca gataccgact tcagaggcgc aggtgatgag
     5461 cttgtctttg gtaatgacgt agcgtgccgg acgcagaccg ttacggtcga ggttacaggc
     5521 agcgaagcga ccatcggaca tcacgatgcc cgccggacca tcccacggct ccatatgcat
     5581 ggagttaaag tcaaagaagg cacgcagttc cgggtccata tccgggttgt tctgccaggc
     5641 gggtggcact aacaggcgca tggcgcggat gatatccatc ccgcctgcca gcagcagttc
     5701 cagcatgtta tccatggaac tggagtcaga accggtttcg ttgacgaatg gcgcggcgtc
     5761 gtgcaggtca gggatcagcg gcgtctggaa tttataggta cgcgcacgcg cccattggcg
     5821 gttaccggtg atggtgttga tttcaccgtt atgcgccaga tagcggaacg gttgcgccag
     5881 cggccagcgc ggtacggtgt tagtggagaa gcgctggtgg aacaggcaaa tggccgattc
     5941 cagacgcagg tccgcaagat ccagataaaa gcgcggcaga tccgccggca tacacagacc
     6001 tttatagatg ttcaccagat tcgacaggct acagacgtag aagtctttgt cggcttcgag
     6061 acgcttttca atgcggcggc gggcgataaa cagacggcgc tccatatcgc gtggacgcca
     6121 gcctgccggg gcgttcacaa aaatttgctc aatgcgtggc agagaggaga gggcgatttc
     6181 acccagcacg ccttcgttag tggggacatc acgccagccc acaatcgaca aggtttcgcg
     6241 ttgcagttct tcttcaacga tgcggcgtgc ggcagcggcg agttcaggat ctttattcag
     6301 gaagagcatc ccgacagcgt agttttttgc taaacgccag ccgcgctcct gcgcaacgat
     6361 gcgaaaaaag cgatccggtt tttgtaacag caagccgcaa ccgtcgccgg ttttaccatc
     6421 ggcgagaatc gcgccacggt gctgcatgcg ggccagtgcg tgtattgcag tacgcactac
     6481 cttgtggcta ggttcgcctt ctatgtgggc gatcaggccg aaaccacagt tatccctctc
     6541 aagggattta tcgtacaaca tatcgtgaac ctcccccagg ctctgcggga accccacttc
     6601 cgaaccgctg cgcacgggca cagaaagagc gtggcgacgg ggtttgcgtg tcatacgcgc
     6661 gcctcgcatt cgcccccttg ttgtcctttt cgcatcggtt aatacggtga agaggggatc
     6721 tcaattactg cataaatatg atgagcaggc tgctcatcca gaaagcttcc agcggatttc
     6781 caacttatcg ggaaagctga tacaggtcaa atggcaagct tattggtaca gaaatgtgct
     6841 ttagcgcaaa attctttatt aaatagctga aattaaaata gattttatct ttaaggctaa
     6901 aaggagagtc gttagagaaa cagtcaattc ctgactgtca taaaggcgtg ttaaccctta
     6961 tcctgccttg aactgacgag aaaaccagca ttttatactg ccttaattgg taatgacgtt
     7021 tcatgctact gtttttgcct aaaatccata aggagataaa aaaatctgac acaagataag
     7081 caaaagtgat ttatgtgatt gtgcatgaaa tttcggtaat tttgattgat tatgcagttg
     7141 ataaaagcag gccgggacga ttgatccagg tcatcgccga attgagctaa aaatggcagg
     7201 ctttggcctc ttttcaaggc gcggtctatc agattatgca gttacagaaa ttagtcaata
     7261 tgtttggtgg tgatctcacc cgtcgttatg ggcaaaaggt gcataagctg acgctgcacg
     7321 gcggttttag ctgccctaac cgtgacggta ccatcgggcg tggtggctgc acattctgta
     7381 atgttgcctc gtttgccgat gaagcgcagc agcatcgttc cattgccgag caactggcgc
     7441 accaggcgaa tttagttaac cgcgctaaac gctatctggc ctactttcag gcgtatacca
     7501 gcacctttgc ggaagttcag gtgctgcgtt ctatgtatca gcaggcggtg agccaggcca
     7561 atattgtcgg tttgtgtgtt ggtacccgcc cggactgcgt gccggatgcg gtgctggatc
     7621 tgctttgcga atataaggac cagggttacg aagtgtggct ggagctgggg ctacaaaccg
     7681 cccacgacaa aacactgcat cgcatcaacc gcggccatga ttttgcctgt tatcagcgta
     7741 caacccagct ggcacgtcag cgcgggctga aggtgtgttc ccacttaatt gtcggcctgc
     7801 ctggcgaagg gcaggctgaa tgtttgcaaa cgctggagcg agtggttgaa accagcgtgg
     7861 acggcataaa gctgcatccg ctgcatattg tgaaaggcag cattatggcg aaagcctggg
     7921 aagcggggcg tttgaacggt attgaactgg aggattatac gctcactgcc ggagaaatga
     7981 ttcgccatac gccaccggaa gtgatctacc accgcatttc cgccagcgcc cgtcgcccga
     8041 cgctgcttgc tccgctgtgg tgcgaaaatc gatggacggg aatggtcgag ctggatcgct
     8101 atctgaatga acatggcgta cagggatcgg cgctgggacg tccgtggcta cctccaacgg
     8161 agtaggtcgt tgaggggaat tccgcatttc tcacacaatt tataacgtaa ctgtcagaat
     8221 tgggtattat tggggcaggt tgtcgtgaag gaattcccta atgaagcaaa ttcgtctgct
     8281 ggcgcagtat tatgttgacc tgatgatgaa gttaggtctg gtgcgcttct caatgttgct
     8341 ggcgctggcc ctcgtcgttc ttgccattgt ggtacaaatg gcggtaacca tggtgctgca
     8401 tggtcaggtc gaaagcattg atgttattcg ttctatcttc tttggtttgc tgattacgcc
     8461 gtgggcggtc tactttctat cggtggtcgt cgagcaactg gaggagtcac gacaacgtct
     8521 gtcacggctg gtgcaaaaac tggaggagat gcgcgagcgc gatttgagcc tcaacgttca
     8581 gttaaaagat aatattgccc agctaaatca ggaaattgcc gttcgtgaaa aagcggaagc
     8641 agaactgcag gaaaccttcg gccaactgaa aattgaaatc aaagagcgcg aagagacaca
     8701 aattcagctc gagcagcaat cctcattctt acgttccttc cttgatgctt cacccgactt
     8761 ggttttttat cgtaacgaag ataaagagtt ttccggctgt aaccgcgcga tggagctgct
     8821 gaccggaaaa agcgaaaaac aactggttca cctgaaacct gctgatgttt actcaccgga
     8881 agccgccgca aaagtcattg aaaccgatga aaaagtgttc cgtcataatg tgtcactgac
     8941 ctatgaacag tggctggatt acccggacgg gcgcaaagcc tgctttgaaa tccgtaaagt
     9001 gccgtactac gaccgcgtgg gtaaacgtca cggtttgatg ggctttggtc gcgacattac
     9061 cgagcgtaag cggtatcagg atgcgcttga acgcgccagc cgcgacaaaa cgacgtttat
     9121 ttccaccatc agtcacgaat tgcgtacacc gctgaacggt atcgtcggcc tgagccgcat
     9181 tctgctggat accgaactca ccgccgagca ggaaaaatat ctcaaaacta tccatgtttc
     9241 ggccgtcacg ctggggaata tctttaacga tattatcgac atggataaga tggaacggcg
     9301 caaggtccag cttgataatc agccggttga tttcaccagc ttccttgccg atctggaaaa
     9361 tctctccgca ttgcaggcgc aacaaaaagg attgcgcttt aacctggagc ctacgctgcc
     9421 attaccgcat caggtcatta ccgacgggac gcgtttacgg cagatcctgt ggaacctcat
     9481 cagtaacgcc gtcaaattca cccagcaagg ccaggttacc gtgcgcgtgc gctacgatga
     9541 aggcgatatg ctgcattttg aagtggaaga ttctggtatc ggcattccgc aggatgagct
     9601 ggataaaatc ttcgccatgt attaccaggt gaaagacagt catggcggta aacctgccac
     9661 cggcaccggt attggtctgg ccgtttctcg tcgtctggcg aaaaatatgg gcggcgatat
     9721 tacggttacc agtgaacagg gcaaaggttc aacctttacg ttgacgatcc acgcaccgtc
     9781 ggtggcagaa gaggtcgatg atgcgtttga tgaagacgat atgcctttac cggcgctgaa
     9841 tgtgctgctg gtggaagaca ttgaactgaa cgtgattgtc gcgcgttctg tgctggaaaa
     9901 attaggtaac agcgttgatg tcgccatgac cggcaaggcg gcgctggaga tgtttaaacc
     9961 gggcgaatac gacctggtgt tgctggatat tcagttgcca gatatgaccg ggctggatat
    10021 ctctcgtgaa ctgacgaagc gttatccgcg cgaggattta ccgccgctgg tggccttaac
    10081 cgctaacgtg ctgaaagaca aacaagagta cctcaatgct ggaatggatg atgtgctgag
    10141 taagccgctt tcggttccgg cgctaaccgc gatgatcaag aaattctggg atacccagga
    10201 tgatgaggag agtacggtga cgacagaaga gaacagtaaa tcagaagcat tgctcgatat
    10261 tcccatgctg gaacagtatc tcgaacttgt aggaccgaag ctgatcaccg acgggttagc
    10321 ggtgtttgag aagatgatgc cgggctatgt cagcgtgctg gagtcgaatc tgacggcgca
    10381 ggataaaaaa ggcattgttg aggaaggaca taaaattaaa ggtgcggcgg ggtcagtggg
    10441 gttacgccat ctgcaacagc tgggtcagca aattcagtct cctgaccttc ccgcctggga
    10501 agataacgtc ggtgaatgga ttgaagagat gaaagaagag tggcgtcacg acgtagaagt
    10561 gctgaaagcg tgggtggcaa aagccactaa aaaatgaccc cggctagacc ggggtgcgcg
    10621 aatactgcgc caacaccagg gaaatcttgg ctgcgccgta aattattatg atgagttaca
    10681 agggcacagc actgtttttc aggccgcacg cagttaagat agcaaaactt aaatgatttg
    10741 ttacatgaat cagttaaatg tgtgatgcgt accattttta ttaaaaaaat taatgtgttt
    10801 cagtcaattg cgaaaggatg atcacaatga agaaaattgg cgtaattctg agcggatgcg
    10861 gcgtctatga cggttctgaa attcatgaag cggtgttgac gttgttagct atttcacgca
    10921 gcggtgcgca ggcggtctgc tttgcaccgg ataagcagca ggttgatgtt atcaaccatt
    10981 taactggcga agcgatgacg gaaacgcgca atgtgctgat tgaagcggca cgaataacgc
    11041 gcggtgaaat ccgtcctctg gcccaggccg atgccgctga actggatgcg ttgattgtgc
    11101 cgggggggtt tggcgcggcg aagaatttaa gcaattttgc cagtcttggt agcgaatgca
    11161 ccgttgaccg tgaattaaag gcgctggcac aagcgatgca tcaggccgga aaaccgcttg
    11221 gttttatgtg tattgccccg gcgatgctgc cgaaaatttt cgatttcccg ctgcgtttga
    11281 ccatcggtac tgatatcgat accgcagaag tgctggaaga gatgggcgcg gagcatgtgc
    11341 cgtgtcctgt cgatgatatc gtggttgatg aagacaataa gattgtcacc accccagcat
    11401 atatgctggc gcagaacatt gcagaagcgg cgagcggcat tgataagctg gtttcccgcg
    11461 tgctggttct ggctgaatga gtaaaagccg cttaacggtg tttagtttcg ttcgccgttt
    11521 tcttttgcgg ttaatggttg tcctcgccgt tttctggggc gggggcatcg cgttgtttag
    11581 cgttgcgcct gttcccttct cagcggtaat ggtcgagcga caggtcagcg cctggctgca
    11641 tggcaatttt cgttacgtgg cacattctga ctgggtcagt atggatcaaa tctcgccgtg
    11701 gatgggactg gcggtgattg ccgcagaaga tcagaaattt cctgagcact ggggctttga
    11761 tgtcgcttcc attgagaaag ccctggcgca caacgagcgc aatgaaaacc gtattcgcgg
    11821 tgcttcaacg atttctcagc agacggccaa aatctctttt tatgggatgg gcgtagctgg
    11881 gtacgaaaag ggctggaagc tggattaacg ctggggatag aaacagtctg gagcaaaaag
    11941 cgtatcctga cggtttacct gaatatcgcc gaatttggcg acggtgtgtt tggcgtcgaa
    12001 gctgcggcac aacgttattt ccacaaaccc gcgagcaaac ttacccggtc ggaagctgca
    12061 ttactggcag ctgtattacc taatccactt cgtttcaaag tctcctcgcc atcgggctac
    12121 gtgcgtagcc gtcaggcgtg gattttacgg cagatgtatc agttaggcgg tgagccgttt
    12181 atgcaacaac accagctgga ttaagcttcg cgcgaagtga gggccagcgt tttgctctca
    12241 cgaggttgca gatctttctg cagggcaata gccagcgccg gttgggcgat aaatcgtttc
    12301 cataaccgct cttgcttacg cagacaacac cacttcgacc aggtcgccag tggaatcaat
    12361 gtgctttcac caatgttgtc gcagacgacc gggataacct ctgactcact gatgcggtga
    12421 cagaggatat tctgcggctt cagcgacatc gtcacgatac ggttatcctg caaataacgc
    12481 ttcagctgtt tcagtagctg gcgcagttgt gcaatgtctt cttcgtaacg gcattgttca
    12541 gcaaattcgg tcagcgtaat ggaaggcttg ccgtcaaaat cggcaatcac atcgtagaca
    12601 taaccggttc cgcaatccgt ctcaacggta ccgtgatagc gcggtattcc actccagtct
    12661 tttaaccggc gaccaagatg cgcgtagtac tttaactcac ggcggatctc tttatcgcca
    12721 ccatcgccac gatggtagac aatcttgata cagcgttggg catcttccgg atgcgcataa
    12781 catttccgat gtcttccggt gcccagggga ctttgttcag ataagcgaat catgccatct
    12841 cctggaaata ttttctgacg atctccagta tggtcgatta acttaaacaa aacctaaaca
    12901 atgcctctta aggttttctt aaggttcttc tgaaagtgaa aagcgcacaa aatgtacaaa
    12961 tgacgttgat tgagaacgat ttcagtacgt gattttctta ttgtcggggg agcttgaagg
    13021 gagttgtatg tcaaagtgat gaagattaat cttcatcaaa accagaatta aagagggcga
    13081 taacggcggc cagtgcttcc tcttcctgtg gaccggtcgc ttcaacttca atctgccgtc
    13141 ctttggcaga atccaacatc agcagcgcaa taacgctgtt ggcttcagcc tcggtgcctt
    13201 cgtcattacg taacagcact tcagcgtcaa aaccctgcat taattcaaac agcttcattg
    13261 caggccgggc atgcatgccc agcttgtttg tgatttcaac agtttgcttg acggtcatgg
    13321 tttacgtttt tccagcgtac gatggcgtga ctggacgttt ttaccgcgcg agcggaagta
    13381 gtctgccagt tgctctgcaa tatacaccga acggtgcttc ccgccggtac aaccaatggc
    13441 gaccgtcagg tagctacggt tgttggtttc cagcataggt aaccatagct caagatagct
    13501 tcgcgtctgg tagataaaat tgtgtacttc tgtgtggcgg tcgaggaacg cggcgacagg
    13561 tttatcaaga cctgtcattg gacgcagttt cggatcccag tgcgggttcg gcaagaagcg
    13621 cacgtcaaag acgtaatctg catcgatagg gataccgtgt ttgaagccga aagactcaaa
    13681 gaccatggtc agttcgcgtt cacgtttacc cagcagacgg gtacgcagca tttcagccag
    13741 ctcgtgaacg gacatttctg aggtatcgac aatcagatcc gctcgcgaac gcagaggctc
    13801 cagcaaatcg ctttctttgt cgatagcact ttccagcgac aggtttttgc tggaaagcgg
    13861 atgcagtcgg cgcgtgtcac tgtaacgacg aattaaggta ttacggtcgg catccaggaa
    13921 cagtagttgc ggtgagaaag cgtcaggcag gttactcatc gcctgttcga atatttctgg
    13981 tgactccggc atattacgaa catcaatgct gacggcggca gaaatctcgc gatcggccag
    14041 agttcgagcc agatcgggta acaacactac gggaaggtta tccacgcagt aaaaacccat
    14101 atcttccagc gcacgcaggg cgacagattt acctgaacct gaacgtccgc tgacgatcat
    14161 cagtaccatg tactgtttct cctcacaacg tctaaaagag acattaccga ataactacgc
    14221 ttcatccgga gtaccttcgg tatccgtaat gatttgatac agctcttcat cgctctgggc
    14281 tgcgcgtaaa cggcggcaga tggttttgtc cgccagacgt ttcgccacca gcgacagagt
    14341 atgcaggtgc gttttagttt ggtctgccgg caccagcagg gcaaaaagta agtccaccgg
    14401 ttggttgtcg atggcatcga aagctatagg cgtttcgagc tgaacgaaaa cgccaacggc
    14461 acgcagagta tcttcttcaa gtttgccatg cggaatggca ataccattgc cgataccggt
    14521 actgcccatt ttttcccgcg tcaggatagc ttcaaaaacc acctgaggcg gcaggctaag
    14581 ttgtttcgcc gccagctcgc tgatgatttc cagggcgcgt tttttgctct gacagtggac
    14641 gcggcttcgc gtacattccc tgttaagaac actgctaagc tgtagagttg tatcattatt
    14701 tgtcataatt tcacctaaga acctgcccac tcaaacagtg gaacaggctc ggagcattaa
    14761 ccgtacaaat ggcccgttgt gccgcacaac aggccgccat gcacatgcta attgcccgga
    14821 caattagtgt tgtttcagtt tatctttatg tttggtcaac tggcgtgcca gcttatcaat
    14881 taagccatca atggcagcgt acatgtcctg accttccgcg ctggcatgaa tttcaccacc
    14941 gtttacatgc agtgttgcat ctgaggtgtg ggtgactttc tccactttca gaacaacata
    15001 gacctggttg attcggtcaa aatattgctc aagtttggca aatttagctg taacaaattc
    15061 gcgcagtgcc tcggtgatct cgacgttatt tccggtaatg ttgagctgca tagtgtcttc
    15121 cttatcggtt gggtcaaacg agctgtttac gctggtttga cggcggaatg gataaagact
    15181 ctcggtactt cgcaacagtg cggcgtgcca ccatgatacc ttgttccgac agcaaagagg
    15241 ttaacttgct gtcgctcaac ggtttcgctg ggttttccgc cgcgattaat ttcttcacca
    15301 gtgcacgaat cgccgtggag gaagcttcgc cgccgccctc ggtattgacg tgactggaaa
    15361 agaaatactt cagttcaaaa atgcctcgtg gactatgcag gtatttttgc gtggtcacgc
    15421 gagatatcgt cgattcatgc atttcgacag cctgggcgat atcggccagt accatcggtt
    15481 tcatatattc ttcaccttgc tcaaagaagg cttgctgctg ttcaacgata cagcgactca
    15541 cgcgcagtag cgtatcgtta cggctttcca gactcttgat caaccatttg gcatcctgca
    15601 gattgctgcg gataaactgg ctgtcaccat cgttgcgcgc gttattgcac atcgaggcgt
    15661 agtgctggtt gatttgcaga cgcggaatgc tgtcactgtt gagttctacc gtccagtgac
    15721 cgttatgctt acgcaccagc acatctggaa tgacatactc aggttcgcca gtctggatcg
    15781 actgcccggg gcgcggatcg agcgactgga tcagattgac ggcttctttc agcacatctt
    15841 ctttcagacg cgtgacgcgc attaaagtgc ggaagtcgtg attggctaac agatcgagat
    15901 gatcgctaat gatcaatctg gcctcttcca gccacggcgt ggtcttatcg aattgggaga
    15961 gttggattag caggcagtca cgcagatctt ttgccgctac accgaccggg tcaaaccgtt
    16021 ggatccgctt aaggacggct tcaacctcgt cgatgtcgat ctcttcatcg cccatacttt
    16081 cgagaatatc ttccagcggg acagtcagat aaccggtttc atcaacggca tcgacgatag
    16141 aggtagcaat agcgcggtca gtgtcggaaa acggtgtcag ctcgacctgc cacatcaggt
    16201 aatcctgcaa ggtctgcgtc gtttcgccct ggtagaccgg cagctcgtcg tcaatgtagt
    16261 caccgctggt gccggatggt gtaccagcgg tgtaaatggt gtcccaactg gcatcgagcg
    16321 gcagctcttc cggcatctct ttttgttcga gcgcgtcggc ggtgtccagc gtttcactgt
    16381 cttgcgtttc gcgggtgtcg atttcttcat gagtgtcgat ttgctcaagc agcggattac
    16441 tctccagcgc ctgctgtagc tcctgctgaa gttccagcgt cgacaactgc aacagacgaa
    16501 ttgcctgttg gagctgtggc gtcatcgcca gttgttggct aagcctgagt tgcaaacctt
    16561 gcttcatgtt cagaatcgta ctctcctgct aaaacgtcgc aaacttctac cctatcagag
    16621 tctgaagtct tccccaaggt atacacgctt aacgtgttcg tcttgtaaga tttctgtagg
    16681 cgtgccgtgg gcgatcaaat gcccctgact gacgatataa gcgcgttcac aaaccgccag
    16741 tgtttcacgc acgttgtggt cagtgatcag cacgcccagg ccgctgtcgc gcaggtgttc
    16801 aatgatgcgt ttaatgtcga taaccgagat cgggtcaacc ccggcaaacg gttcgtcgag
    16861 cagaataaat ttcggattcg cagccagtgc gcgggcaatt tctacacggc gacgttcacc
    16921 cccggagagt gactgcccca tgctgtcacg caggtgctca atgtgaaact cttccatcag
    16981 ctcgttcgcg cggtcttcac gttgttcagc agacaagtcg tcacgaattt gcagtaccgc
    17041 catcaggtta tcgtaaacgc tgaggcgacg gaaaatggag gcttcctgtg gcagatagcc
    17101 gataccgcgg cgcgcgcgtg catgcagagg cagcagactg atatcgtcat catcaataat
    17161 gatgttgccc gcatcgcgcg gcacaatgcc tacaaccatg tagaaagtgg tggtcttacc
    17221 ggcaccgttt ggccccagca gaccgacaat ttccccggag ttgacggtca ggctgacgtc
    17281 ttctaccacg cgacggcctt tataggcttt tgcaaggttc tttgcagtta atgttgccat
    17341 aacgaattaa ttacccttct tctgtgccgg ggtctggcct ttgttgtttt tgtcctgcag
    17401 ctgcgacggc accagaacgg tggttacgcg cttgcctttg tcgctgaaag cctgcatttt
    17461 ctgctctttc accaggtaag tgatcttatc gcccttaatg ttgctatcga cctgctgcag
    17521 ataagcatta cccgtcagaa cgacaaaatc ttttgccagt tcgtagtgca tctgggaagc
    17581 gtgaccttca acgggtttac cgttgtcctg catctggtag aacgttgccg gtttaccgta
    17641 tccgtcaatc acttctttac cttgttcgcc gcccggacgg gtaacgacca ctttgtcggc
    17701 attaattttg atggtgccct gggtgacgat gacattaccg gtaaaggtaa ccacgttgcc
    17761 ttgcatatca agagattgct ggtccgattc aatgtggatc ggctgatcag tgtctccggt
    17821 tacggcaaat gccggaatgc tggcggccag aagtgagctg gcaagcacaa gattaaggct
    17881 gagtttgttt gttttgaatt tcataggatg ttctaacctt ttcaatcagc tcggcgttct
    17941 tgctgcgtaa gttgccgcgc attttcagac cgctggagtt aaatgttgtt ccgtataacg
    18001 tgacgaggtc ttcagaggta acatcctgcg tcaccagatt gatctgcgcg ttatccgtcg
    18061 tgattctgcg aagttgagag tctggcacaa gtgcgttgac ttcaacgtgt ccatataaat
    18121 agagcatccg gtcattggtc agcttggctt tatctgcttt tacggaccat gtcgggattt
    18181 tatccttatc aaacgtggta agtaccggct gcgtaaacca cgaaacggcc tgatcggaat
    18241 aatattcaac gtgttgagca atcaatcgat agcttagtgc cccttctggg ttatagacga
    18301 gcgtgtccgt atgctcgctt ttataggtgg gatcattgtt gttgacgacc acctgggcgg
    18361 tatcgtcttt ttcggccata ttaatgccga tcatcaccag aaccgccagt gatagcacaa
    18421 tgataaccca acgtctggct ttactcatat cgattgccct ttggcttcat ccagtttgcc
    18481 ctgcgccagg agtaataagt cgcaaacttc gcgcactgcg ccacgaccgc cagcaatgcg
    18541 cgtcacgtaa tcggcgcgcg ggatcaacag tggatgcgca tcggccacgg cgacgcttaa
    18601 acccactttt tccattaccg gccagtcgat gagatcatcg ccaacataag ccacattttc
    18661 cggggcaatc gccaattttt ccagcaaatc gctaaaggcg atcagtttgt ttgactgccc
    18721 ctgatacaag tgagtgatcc ccaatgtggc acaacgatct tctaccagtt tagcctttcg
    18781 cccggtaatg atagcgactt caatatcaga ggtgagcgca caacgaatgc cataaccgtc
    18841 acgaacattg aacgctttca gctcttcgcc gttattgccc atataaatca ggccatctga
    18901 cagtacgcca tcaacgtcga ggatcagcag acgaatgttc tctgcttttg ccataacgtc
    18961 ggcgctgaca gggccgtaac aggtcgcaag cgacgcacct gctttgctca ttgttgttta
    19021 tccttgaatc tttacactac gcctgcacgc agtaaatcat gcatatgtaa cacaccgagt
    19081 aaatggtcgc catcggcaac catcacggag gtgatatggc gggactgcat taagttcagt
    19141 gcctcaacgg ccagaatgcc agggcgcaca cgtattcccc ccggcgtcat cacatcggca
    19201 atacttaact gacgaacatc cacgcccata tcgaagacac ggcgtaaatc accgtcggta
    19261 aagatgcctt caatcatcat attgtcatcg caaatgacag tcataccaag atttttgcgg
    19321 gtaacttcca gcaatgcgtc acgcagactg gccgttttct taacatgcgg gatctcatcg
    19381 cccgtatgca taatatcgtt tacgcgcagc agaagtttac gacccagtgc gccgcctggg
    19441 tgtgagagcg caaaatcttc agcagtaaag ccgcgtgctt ttaacagcgc gacagcgagg
    19501 gcatcgccca taaccagcgt ggcggtggtg ctgctggtcg gtgccagccc taacggacag
    19561 gcttctttcg ctactttaac acacagatgc acatctgcgg cgcgcgccat gctgctctcc
    19621 gggcgaccgg tgatgcagat taacggtacg tgaagacgct taagcactgg aattaaggcc
    19681 gtgatttcgc tggattcacc tgagttagag atagcaatca ccacatcctg tggggtgacc
    19741 atgcctaaat caccatgcgc ggcttcacta ggatggacga aaaatgaagg tgtaccggtg
    19801 ctggcaaacg ttgcggccat tttgcgcccg atgtgccccg atttccccat ccccatgacg
    19861 acaactttcc ctttacacca gaacatcttt tcacaggcaa gcgtgaaatt ctgattgatg
    19921 tattgatcaa gctccgccag gcattcacgt tcaatcgcca ggacttcttt acctgcttgc
    19981 tgaaagtcaa aacccggttg taactctacg tgcgacataa tgcgtttcca gttattcaac
    20041 gagtattggc gataaccagt acaacatcgc cagccatacg ataaatccgc cagttaataa
    20101 tacccctaca ccacggcccg gttgcgggga gcgccgccag cacagcaacg caaaaataat
    20161 gctcaccagc aacatcacgc tgtagtcacg actgtacgcc agtggatcaa tctctcctgg
    20221 cgttatcagc gcgggtaaac ccaacacgat gacaatatta aaaatgtttg cgccaatgat
    20281 atttccgaca gcaatgtcgt tttcaccttt gcgaaccccc gctattgcgg ttgccagttc
    20341 cggcagactg gttccgatag caattgccgt cagagccatc gtcaactcgc tgatggcaaa
    20401 gtaattcgcc agcaccgtgg cgttatcaac caccatccgc gtggccactg gcatgatgat
    20461 aagcgcaatg ccgagccata aaaacgcgac gggcaatccg ccgtcacgcg gcagctctgc
    20521 aagctgctct ctggtcaggc tgtcagtccc ctgacgtgca agtttaacaa tgaacagcag
    20581 ccatagcaca gccagaaaga ggagaaagat accatcgctt cgactaagtt gtccgtcata
    20641 gagtacggaa ccggccacca cgctgaccaa caacattaag ggtaattcac ggcgcagaac
    20701 atcggaatgg acggtaaaag gacgaaccag cgcagccaga ccgaggatca gcaatatatt
    20761 gataatgttt gagccgaggg ctgtaccaac ggctaaatcg cgttgttcgt gcagagacgc
    20821 ggcaagcgag acgatgattt ctggtaacga tgtaccaata ctgaccaccg tcatgccgat
    20881 gatcagcggc gggatgccaa aggttcggca aagaatagac gcggcaaaaa ccaggcggtc
    20941 ggcactgtaa acgaccaaaa gtaaaccaac aattaacagt gccgtagcta aaagcatcta
    21001 acgtcctttc ttcaggtata ctcgccggtc cgctgaagat tttcagaaag ccgtaacgga
    21061 ttcttaattt tgactttatg cggctaaaaa gtaaaacaaa tgccagcttt cgctaaccac
    21121 ggcgggtaat attctgtaaa tatgttgggt tcaaggttaa attgagcgcc atgcttagaa
    21181 aatcaacgca agacgaaggg tgaattatgg agcagtctgt ggcgaattta gtcgatatgc
    21241 gcgatgtcag ttttacgcgt ggcaatcgct gcatcttcga taatatttcc ctgaccgtgc
    21301 cgcgagggaa gatcacggcg atcatggggc catcgggcat cggtaaaacg acgctactcc
    21361 gtctgattgg cgggcaaatc gcaccagatc atggtgagat ccttttcgat ggtgagaata
    21421 ttccggcgat gtctcgttcg cgcctgtata cagtgcgtaa acggatgagc atgttatttc
    21481 agtccggggc gttgttcact gatatgaacg tatttgacaa cgtcgcctat ccactgcgcg
    21541 aacataccca acttcccgcg ccattgttgc atagtacggt gatgatgaag ctggaggccg
    21601 tggggctgcg tggagcggct aaactaatgc cttctgaact ttccggtggg atggcgcggc
    21661 gtgcagcgct ggcgcgtgcg attgcgctgg agccggatct catcatgttt gatgaacctt
    21721 ttgttgggca agatcccatc accatgggcg tactggtgaa gctgatttct gagctgaaca
    21781 gcgcgctggg cgtgacttgt gtggtggttt ctcacgatgt gccggaagtg ttaagtattg
    21841 cggatcacgc ctggatcctg gcggacaaaa aaattgtcgc tcatggcagt gcccaggcgt
    21901 tgcagacgaa tcctgatccg cgcgtacgtc agtttctgga cgggatagct gacgggcctg
    21961 ttccgttccg ctatcctgcc ggcgattatc acgctgatct tttaccaggg agttaagcca
    22021 ctcatgctgt taaatgcgct ggcgtcgctc ggacataaag ggattaaaac cctgagaacg
    22081 ttcgggcggg ccgggttaat gttattcaat gcgctggtcg gcaaaccgga atttcgcaaa
    22141 catgcgccgc tgctggtgcg ccagctctat aatgtcggcg tcctgtcgat gctgattatt
    22201 gtagtttctg gcgtgttcat cggaatggtg ttggggctgc aaggttatct ggttctgacc
    22261 acttatagcg cggaaaccag tctgggtatg ctggtggcgt tatcgctact gcgtgaactg
    22321 gggccggtgg ttgccgcgct gttgtttgcc gggcgtgctg gttcggcgtt aaccgcagaa
    22381 atcggcctga tgcgcgctac agagcaactc tccagtatgg agatgatggc ggtggatccg
    22441 ctgcgtcggg ttatttctcc ccgtttctgg gctggggtta tttcattacc actgttgacg
    22501 gttatcttcg tcgccgtggg gatctggggc ggatcgctgg tcggcgtcag ttggaaaggc
    22561 attgatagcg ggttcttctg gtcggcaatg caaaatgccg tcgactggcg tatggatctg
    22621 gtcaactgtc tgattaagag cgtggtgttc gccatcacgg tgacgtggat ttcgttgttt
    22681 aacggctacg acgccatccc gacgtctgcc gggattagcc gggcaaccac tcgcaccgtt
    22741 gtccactcgt ctctggctgt tctggggctg gattttgtgc tgaccgcatt gatgtttggg
    22801 aattgagttc atgcaaacga aaaaaaatga aatttgggtg ggtatctttt tattagcagc
    22861 actgctggcg gcgctgtttg tttgcctgaa ggcggcgaac gtgacgtcca tacgtactga
    22921 atcgacctac acgctttatg cgacgttcga taacattggc ggcctgaaag cgcgctctcc
    22981 ggtcagcatt ggtggtgttg ttgtgggccg ggtggcggat attacgctgg acccgaaaac
    23041 ctatctgccg cgcgtaacgc tggaaattga acaacgttat aaccacattc ctgataccag
    23101 ttcgctgagc attcgtactt ccggcctgct gggagaacaa tatctggcat taaacgtcgg
    23161 ttttgaagac ccggaactgg ggactgctat cctgaaggat ggcgatacaa ttcaggacac
    23221 caagtctgcg atggtgctgg aagatctcat tggtcagttc ctttacggta gtaaaggcga
    23281 tgacaataag aatagtggcg atgcgccagc tgctgcgcca ggtaataatg aaaccactga
    23341 acctgtgggt acaacgaaat aatttcagga gaaccgacgc atgtttaaac gtttaatgat
    23401 ggtcgctttg ctggtgattg cacctctgag tgcggcaacc gcggcagacc agaccaatcc
    23461 ttataagctg atggacgagg cggcgcagaa aacgttcgat cgcctgaaga atgagcaacc
    23521 gcaaattcgg gccaacccgg attatctgcg taccattgtt gatcaggaac tgctgccata
    23581 cgtacaggtg aaatacgccg gtgcgctggt gctgggccag tattacaaga gtgcgacccc
    23641 tgctcaacgt gaagcctact ttgccgcttt ccgtgagtac ctgaagcagg cttacggtca
    23701 ggcgctggcg atgtatcacg gtcaaaccta tcagattgcg ccagaacagc cgctgggcga
    23761 taaaaccatt gtgcctattc gcgttaccat tattgacccg aatggccgtc cgccggtgcg
    23821 tctggacttc cagtggcgta aaaactccca gacgggaaac tggcaagctt acgacatgat
    23881 tgctgaaggc gtcagtatga tcaccaccaa acaaaacgag tggggaacgc tgctgcgtac
    23941 caaaggtatc gacggcctga ctgcgcaact gaaatcgatt tctcaacaga aaatcactct
    24001 ggaagagaaa aaataatgag cgagtcactg agctggatgc agacgggtga cacgctggcg
    24061 ttatccggag agctggatca ggacgttctg ctaccgcttt gggaaatgcg tgaggaggcg
    24121 gtgaagggga ttacctgcat cgatcttagc cgtgtgtccc gcgtggatac ggggggactg
    24181 gcactgctgc tccatcttat cgatctggcg aaaaagcagg gcaacaacgt gacgcttcag
    24241 ggggtaaacg acaaagtgta taccctcgcg aaattgtata atttgcctgc tgatgttctg
    24301 cctcgttaaa ttttttcagg ccggatatgg cgttaaccgc ctgtccggca aacctgcctg
    24361 caagtcgagc cttgtctgat ttatcagcga ggctttttgc ttatttatgc cagcgcaact
    24421 ttgctctaag atgtttcgct ggtttattaa ctgatgattg aagatcccat ggaaaataat
    24481 gaaattcaga gcgtgttgat gaacgctctc tccctccagg aagtccacgt ttccggcgat
    24541 ggcagccact ttcaggttat tgccgtgggt gagttgtttg acggtatgag tcgggttaaa
    24601 aaacagcaga cggtctatgg cccgctgatg gaatatattg cggataaccg cattcatgct
    24661 gtgtcgatca aagcgtatac ccctgcggag tgggcgcgcg atcgcaaact gaacggcttt
    24721 tgagctatgg gcgattcgcc ggtagcggat atgaattgtt aactgagaac aaactaaatg
    24781 gataaatttc gtgttcaggg gccaacgaag ctccagggcg aagtcacaat ttccggcgct
    24841 aaaaatgctg ctctgcctat tctttttgcc gcactactgg cggaagaacc ggtagagatc
    24901 cagaacgtcc cgaaactgaa agacgtcgat acatcaatga aactgctaag ccagctgggt
    24961 gcgaaagtag aacgtaatgg ttctgtgcat attgatgccc gcgacgttaa tgtattctgc
    25021 gcaccttacg atctggttaa aaccatgcgt gcttctatct gggcgctggg gccgctggta
    25081 gcgcgctttg gtcaggggca agtttcacta cctggcggtt gtacgatcgg tgcgcgtccg
    25141 gttgatctac acatttctgg cctcgaacaa ttaggcgcga ccatcaaact ggaagaaggt
    25201 tacgttaaag cttccgtcga tggtcgtctg aaaggcgcac atatcgtgat ggataaagtc
    25261 agcgttggcg caacggtgac catcatgtgt gctgcaaccc tggcggaagg caccacgatt
    25321 attgaaaacg cagcgcgtga accggaaatc gtcgataccg cgaacttcct gattacgctg
    25381 ggtgcgaaaa ttagcggtca gggcaccgat cgtatcgtca tcgaaggtgt ggaacgttta
    25441 ggcggcggtg tctatcgcgt gctgccggat cgtatcgaaa ccggtacttt cctggtggcg
    25501 gcggcgatct ctcgtggcaa aattatctgc cgtaacgcgc agccagatac tctggacgcc
    25561 gtgctggcga aactgcgtga tgctggagcg gacatcgaag tcggcgaaga ctggattagc
    25621 ctggatatgc atggcaaacg tccgaaggct gttaacgtac gtaccgcgcc gcatccggca
    25681 ttcccgaccg atatgcaggc ccagttcacg ctgttgaacc tggtggctga agggaccggg
    25741 ttcatcaccg aaacggtctt tgaaaaccgc tttatgcatg tgccagagct gagccgtatg
    25801 ggcgcgcacg ccgaaatcga aagcaatacc gttatttgtc atggtgttga aaaactttct
    25861 ggtgcacagg ttatggcaac cgatctgcgt gcatcagcaa gcctggtgct ggctggctgt
    25921 attgcggaag ggacgacggt ggttgatcgt atttatcaca tcgatcgtgg ctacgaacgc
    25981 attgaagaca aactgcgcgc tttaggtgca aatattgagc gtgtgaaagg cgagtaatcg
    26041 tctgagagct ggcagccccg ttcgcggggc taccgccaga ccactcattt tttcggttta
    26101 gtgtagctac gcatcaactg cgttctgtcg ataaactcat gggtctgcgg atcatggtag
    26161 cgtgatggcc agataaccca ggggtcagtt cccagggctt tcgcaataat catctctcct
    26221 ttcggccatg ggcgcgataa tgcgtttgcc agcgttgagg aactcaaacc atttctgcga
    26281 gattccgccg ccattgacgt tcctttcttg cgcaaacctg caatgatgtc agcgggatgc
    26341 cagtcaatga aattactttc catctttcct cactccttaa gttagtcgct ttcattcaga
    26401 acacttcttc tgtcgggcat cactatactc ctgatggtct attgcgctaa aaagttcctg
    26461 taaagagtgt gaatattcag aatatataaa gattcatcgc ccttttttca ggtgttatag
    26521 cgctgaatta gtgtttttat ggaatttact ggaatcatcc cgcgcactgc gcgggatgaa
    26581 gggaggggat taacgatcgc gttgaacagc gatgtgcgcg aggccgatga gtgcttctcg
    26641 ccaaggggtg tccgggagca cctgtaacgc tgcgatggct ttgtctgctt cttcctcggc
    26701 gcgctgacgc gtccattcaa gagatccaca agcgttcatt gcttccagaa ccggttccag
    26761 aagatggcga ccgttacctt gttcgatggc ggtacggatc atctgtgcct gttctggtgt
    26821 gccatgatgc atcgcatgca gcagcggcag cgtcggttta ccttcgttca gatcgtcgcc
    26881 gacgttttta cctaactgtt cgccatcggc attgtaatcg agtaaatcgt cgatcaactg
    26941 gaaagcagtg ccgagatagc gcccataatc ctgcaggcct ttctcctcct ccggcgtaca
    27001 gccagccaga atcccggaac actgcgcggc agcctcaaac agacgcgcgg ttttgctata
    27061 gataacgcgc atgtagtttt cttcagtgat gtccggatcg ttaacgttca tcagttgcag
    27121 aacttcacct tctgcgatga cgtttacagc ttctgacatg acttccagca ctttgagtga
    27181 accgaggctg gtcatcatct ggaacgcgcg ggtataaata aaatcgccta ccagcacgct
    27241 ggcggcattg ccaaatgcgg cgttggcggt agctttaccc ctgcgcatat ctgattcatc
    27301 cacaacgtcg tcgtgtagca gagtcgccgt gtggataaac tcgatcaggg cagcaatggt
    27361 gacatgcgca tttccctcat agccaacagc tcgtgcagcc agtacagcaa tcatcggacg
    27421 aatacgttta ccgccgccgc tgacgatgta atagcctaac tgattgatca gttggacgtc
    27481 ggaattaagc tgctcaagga ttgccgcatt aacacccgcc atatcttgcg cggttaactc
    27541 attgattttt tctaaattca tcgcaaaagc cgggcttttc gccccgatta ccttactgtg
    27601 aacaaaggca acttagggtt tatggttagg aatatagggt aattgtactg aaaaaatggc
    27661 acagataaac gttaccgtac aagttgtgtt tttttcttcg tgtattgact gtagcacttg
    27721 tcaaaggcgt gcgttttgcg taatattcgc gccctattgt gaatatttat agcgcactct
    27781 gaatcattga aaaggtgtgc gcggaagcgg agttttatat gtacgcggtt ttccaaagtg
    27841 gtggtaaaca acaccgagta agcgaaggtc agaccgttcg cctggaaaag ctggacatcg
    27901 caactggcga aactgttgag ttcgctgaag tgctgatgat cgcaaacggt gaagaagtca
    27961 aaatcggcgt tcctttcgtt gatggcggcg taatcaaagc tgaagttgtt gctcacggtc
    28021 gtggcgagaa agttaaaatc gttaagtttc gtcgtcgtaa acactatcgt aagcagcagg
    28081 gccatcgtca gtggttcact gatgtgaaaa ttactggcat cagcgcctaa gacctgagga
    28141 gagatttcaa atggcacata aaaaggctgg cggctccaca cgtaacggtc gcgattcaga
    28201 agctaaacgc ttgggcgtta agcgtttcgg tggcgaatcc gttctggcgg gtagcatcat
    28261 cgttcgtcaa cgtggtacca aattccacgc tggcgctaac gtaggttgcg gtcgtgacca
    28321 cactctgttt gctaaagcag acggtaaagt gaaattcgaa gttaaaggcc cgaaaaaccg
    28381 taaatttatc agcatcgaag ctgaataagt ttttcgcgtc ccggtaacgg atgaaagccc
    28441 cgcaacacgt tgcggggctt tttacattcg ataaccggta aaaatgcgca tggtgcgacg
    28501 ttaatccgca aatgcgcttt gcagggaata cggtatgaag cagcaggcag gcattggcat
    28561 tcttttggcg cttaccacag caatttgctg gggggcgttg ccaatcgcaa tgaagcaggt
    28621 gctggaggtg atggaacctc cgacaatcgt gttttaccgt ttcttgatgg cgagtattgg
    28681 ccttggtgcc attcttgcgg tgaagaagag gttgccgcca ttacgcgtgt ttcgtaagcc
    28741 acgctggttg attttgttgg cagtggcgac cgccgggctg tttgggaact tcatcctgtt
    28801 cagctcatcc ttgcaatacc tgagtccgac cgcttcgcag gtgattgggc aactctcgcc
    28861 agttggcatg atggttgcca gcgtatttat cctgaaagag aaaatgcgca gcactcaggt
    28921 tgtaggggca ttgatgctcc tgagcggtct ggtgatgttt tttaacacca gtctggtcga
    28981 gatatttaca aagctcaccg attacacctg gggagttatc tttggggtcg gtgcggcgac
    29041 ggtttgggtg agttatggcg tggcgcaaaa ggttttattg cgtcggctgg cctcaccgca
    29101 gatcctgttt ttactgtaca ctttatgtac aatcgcgctc ttccctctgg caaagcctgg
    29161 agtgatagcg cagcttagcc actggcagct cgcatgttta attttttgcg gactgaatac
    29221 cttggtagga tatggcgcct tggcggaagc gatggctcgc tggcaggcag cgcaggtgag
    29281 cgcgatcatc acgctcaccc cactgtttac gctgtttttt tcagatcttt tatcactggc
    29341 ctggcccgat ttcttcgcca gaccgatgtt aaacctttta ggttatctcg gtgcgtttgt
    29401 cgtggttgcg ggcgcgatgt attccgccat tggtcatcgt atttggggcg gattacgtaa
    29461 gcatacaacg gtggtatcgc aaccccgcgc aggcgaatga tttacggaga ataaaatgaa
    29521 gtttgttgat gaagcatcga ttctggtcgt tgcaggtgat ggcggtaatg gttgcgtgag
    29581 cttccgccgc gaaaagtata ttccgaaagg cggcccggat ggcggcgacg gcggtgatgg
    29641 tggtgacgta tggatggaag ccgacgagaa cctgaacacg cttatcgatt atcgttttga
    29701 aaaatctttc cgtgcagagc gcggtcagaa cggcgcaagc cgcgattgta ccggtaagcg
    29761 cggtaaagac gtgacgatta aagtgccggt aggtacgcgt gtaatcgacc agggtactgg
    29821 tgaaaccatg ggtgatatga ctaaacacgg tcagcgtctg ctggttgcta agggcggctg
    29881 gcacggtctg ggcaataccc gtttcaaatc gtcagttaac cgtacaccac ggcagaaaac
    29941 taacggtacg ccgggcgata agcgtgagct gctgctggag ctgatgctgc tggctgacgt
    30001 cggtatgttg gggatgccaa acgcgggtaa atcgaccttt attcgtgcgg tatcggcggc
    30061 taaaccgaaa gtggcggatt atccgtttac cactctggtg ccaagtctgg gtgtggtacg
    30121 aatggacaac gaaaagagct tcgttgttgc cgatattcca ggactgattg aaggcgctgc
    30181 ggaaggcgca ggtctgggca ttcgcttcct gaagcacctg gaacgttgcc gcgtcctgtt
    30241 gcacctcatc gatatcgatc cgattgacgg caccgatccg gttgaaaacg cgcgtattat
    30301 tatcagcgag ctggaaaaat acagccagga tctggcggcg aaaccgcgtt ggttagtctt
    30361 caacaagatc gatctgctgg ataaggcaga agccgaagag aaagcgaaag cgatcgctga
    30421 agcgctgggc tgggaagata aatattatct gatctctgcg gcgagcggac tgggcgtgaa
    30481 agatctctgc tgggatgtga tgacctttat cattgaaaac ccggtcgtgc aggctgaaga
    30541 agcgaaacag ccagagaaag tcgaattcat gtgggatgat tatcaccgtc agcagcttga
    30601 agagattgct gaagaggatg atgaagactg ggatgacgac tgggacgaag acgacgaaga
    30661 aggcgttgag ttcatttaca agcgttaatc actaatgacc aatgccaaat aagcctgatg
    30721 cgctacactt atcaggccta caagatgatt gcaatatatt gaatttgcac gattttgtag
    30781 gccggataag gcgttcacgc cgcatccggc ataaacaaag cgcactttgt caataatctg
    30841 aaaccccggc catgcgccgg ggtttctttt tgactaattg ttctgataaa tatctttgta
    30901 caaacggctt tcaaaacgca ctaacggaat acggcgatta cgctgatccg cgggttttac
    30961 tgcatagcca gaaagatatt gtacaaatgc cattcgttgc ccgctcgctg tggtaatgaa
    31021 tcccgccagg ttatataccc cctgcaacga accggttttc gctgagactt tgccatccac
    31081 gcccgcctga tgcagacctg cacggtactg caaagagccg tcatagcccg caagcggcag
    31141 catggagata aagttaagtt cattgtcgtg ttgggcaatg tattgcagca cctgcatcat
    31201 ggtggcgggg gcaatcaggt tatgccgcga aagccctgaa ccatcggcaa taatggtgtt
    31261 accaatatcg acaccggctt gctggcgcag gatctgacgc acggcgtccg acccagcccg
    31321 ccatgttcca ggcacattga agcgcgcatg gccgatcatg cggaaaaccg tatcggcgat
    31381 catgttgtcc gactttttca gcataatctt aagcagatcg tgcagcgggg ccgactgttt
    31441 actggcaact accgttccag gttcgttaac ctgagtctgg cgcagcagtg ttccgctcca
    31501 ggtgataccc gcctgtttta actcatcttt cagaattgca ccggcatagc tggctccatc
    31561 ctgcacggca aaagccaacg ggagcggctc agaacgttgt ggcaggcatc ccgtcagcgt
    31621 aaagcggttc aggtcgcttg gcaccacatc cagttcgcag tattgcgctt cggcagaacc
    31681 acgggggagg gtgcgtacct ggctgaacat cgtaacgggg taataagatg ccacgcgtat
    31741 aaaagccata tcaccaggct ttggggcact gtagagcgag acggagaaac agttgcggtc
    31801 aactatggcg gcagcaggcg gagcgctaaa gcattgtgtc atgtcattcc atggccagcc
    31861 gggggcttta tcgtggctgg cgaaaatgga ggtatctatc aacacattgc catcgatttg
    31921 gttgacgcca gattttttca aagtcgcgac catattgcga atatcctgac gttttaacgt
    31981 cggatcggca ccaaatcgcg ccactaagtc acccttaagt acgccgtttt ccacattgcc
    32041 tttggtttca agcgtcgtgg tgaaacgaaa atcggggccg agttgaatca acgccgccag
    32101 cgcagtaatc actttctggg tactggcggg cagcgccatc tgctgactgt ggtaatcaat
    32161 agcgggggcc gacgcaccga ctttttgcac catcagggca aggttggcac ctgcggggag
    32221 ttgagtaacg tactcatcaa cattcgcggc ctgaacactg aacgctatac agctggtcaa
    32281 tccgatgata aatctggaaa atcgcataat ctcgcgctaa caacctggaa tcgagccgtc
    32341 atactacggc gcaacgccct ataaagtaaa cgatgaccct tcgggaactt cagggtaaaa
    32401 tgaccatcaa aatgtgaatt gtagctgacc tgggacttgt acccgggtcg gtattttttt
    32461 gcttctggtc ccggtgagga gttatgccgg gcaggccgaa cagccggggt gggtgaagac
    32521 ttgccccatc aggaatattc aagaggtata acaaatgcaa gctattccga tgaccttacg
    32581 cggcgctgaa aaattacgcg aagagctgga ttttctgaaa tctgtgcgcc gtcctgaaat
    32641 cattgctgct atcgcggaag cgcgtgagca tggcgacctg aaagaaaacg ccgaatacca
    32701 cgcagctcgt gaacagcagg gcttctgcga aggccgtatc aaagacatcg aagccaagct
    32761 gtcaaacgcg caggtgattg atgtcaccaa aatgcccaac aatgggcgcg ttatttttgg
    32821 tgctaccgta acggtgctga atctggattc tgacgaagaa cagacttatc gcatcgttgg
    32881 cgatgacgaa gctgacttta aacaaaacct gatttctgta aactcgccta ttgctcgtgg
    32941 cctgatcggc aaagaagaag atgatgttgt ggtcatcaaa acgccgggcg gcgaagtaga
    33001 atttgaagta attaaggtgg aatacctgta agaattaccc aatactcaag atgttgatgt
    33061 attgtaaaga aaggaaaaag gccgctctgc ggccttttat caacgaacag agcgtggcat
    33121 tttgctctcc tgcctgcgga aaacccctcg ttttacacag caaatgtgtg taactttagg
    33181 ataatcttag cgtggcagcg agattttacg ttctttagtt gggcgataaa gcaccagcgt
    33241 tttaccgatg acctgtacat tacaggcgcc ggtttcgcgc acgatagctt ccacgatcaa
    33301 ggtttttgtt tcgcgatctt cggtggcgat tttcaccttg atgagttcat ggtgctctaa
    33361 cgcttgttca atctcggcca gcaccccttc ggtcaaacca ttactgccaa gcagaacaac
    33421 tggcttgagc ggatgtgcca gacctttcag gtgctgtttt tgtttagtac tcagattcat
    33481 cgtatttttt gcttacgttg ggattgaaaa cgggtcattc taccgccatc tcccatatat
    33541 caccaaatag gcgcgtaaaa atttacgcaa ttggttacga tgagttatcc ccatgggaaa
    33601 gttaaatgac aggtaagaag cgttctgcca gctccagccg ctggcttcag gaacacttta
    33661 gcgataaata tgttcaacag gcacagaaaa agggattacg ttcccgtgcc tggtttaaac
    33721 ttgatgaaat acagcaaagt gacaaactct ttaaaccggg aatgacggtt gtcgaccttg
    33781 gtgctgctcc gggtggttgg tcacaatatg tggtcaccca aattggcggc aaaggccgca
    33841 tcatcgcttg cgatctttta cctatggatc ctatcgttgg tgtggacttt cttcagggcg
    33901 attttcgtga tgaactggtg atgaaagcac tgctggagcg cgttggcgac agcaaagtcc
    33961 aggttgtcat gtccgatatg gcaccaaaca tgagcggaac accggcggtg gatatccccc
    34021 gagccatgta tctggtggaa ctggcgctag aaatgtgtcg tgatgtatta gcgccaggtg
    34081 gcagttttgt agtgaaggtg ttccagggcg aaggtttcga tgagtatcta agggaaattc
    34141 gctccctgtt tacgaaggtc aaagttcgta agccggactc ttctcgtgca cgttcgcgag
    34201 aagtgtatat tgtagcgacc gggcgtaaac cctaaccggg agatttcaga cgaaagtttg
    34261 aaagatgctg gatatagagt atcctgacgc tgtttttaac acagttgtaa taagaggtta
    34321 atcccttgag tgacatggcg aaaaacctaa tactctggct ggtcattgcc gttgtgctga
    34381 tgtcagtatt ccagagcttt gggcccagcg agtctaatgg ccgtaaggtg gattactcta
    34441 ccttcctaca agaggtcaat aacgaccagg ttcgtgaagc gcgtatcaac ggacgtgaaa
    34501 tcaacgttac caagaaagat agtaaccgtt ataccactta cattccggtt caggatccga
    34561 aattactgga taacctgttg accaagaacg tcaaggttgt cggtgaaccg cctgaagaac
    34621 caagcctgct ggcttctatc ttcatctcct ggttcccgat gctgttgctg attggtgtct
    34681 ggatcttctt catgcgtcaa atgcagggcg gcggtggcaa aggtgccatg tcgtttggta
    34741 agagcaaagc gcgcatgctg acggaagatc agatcaaaac gacctttgct gacgttgcgg
    34801 gctgcgacga agcaaaagaa gaagttgctg aactggtaga gtatctgcgc gagccgagcc
    34861 gcttccagaa actcggcggt aagatcccga aaggcgtcct gatggtcggt cctccgggta
    34921 ccggtaaaac gttgctggcg aaagcgattg caggcgaagc gaaagttccg ttctttacta
    34981 tctccggttc tgacttcgta gaaatgttcg tcggtgtggg tgcatcccgt gttcgtgaca
    35041 tgttcgaaca ggcgaagaaa gcggcaccgt gcatcatctt tatcgatgaa atcgacgccg
    35101 taggccgcca gcgtggcgct ggtctgggcg gtggtcacga tgaacgtgaa cagactctga
    35161 accagatgct ggttgagatg gatggcttcg aaggtaacga aggtatcatc gttatcgccg
    35221 cgactaaccg tccggacgtt ctcgacccgg ccctgctgcg tcctggccgt ttcgaccgcc
    35281 aggttgtggt cggcttgcca gatgttcgcg gtcgtgagca gatcctgaaa gttcacatgc
    35341 gtcgcgtacc attggcaccc gatatcgacg cggcaatcat tgctcgtggt actcctggtt
    35401 tctccggtgc tgacctggcg aacctggtga acgaagcggc actgttcgct gctcgtggca
    35461 acaaacgcgt tgtgtcgatg gttgagttcg agaaagcgaa agacaaaatc atgatgggtg
    35521 cggaacgtcg ctccatggtg atgacggaag cgcagaaaga atcaacggct taccacgaag
    35581 cgggtcatgc gattatcggt cgcctggtgc cggaacacga tccggtgcat aaagtgacga
    35641 ttatcccacg cggtcgtgcg ctgggtgtga ctttcttctt gcctgagggc gacgcaatca
    35701 gcgccagccg tcagaaactg gaaagccaga tttctacgct gtacggtggt cgtctggcag
    35761 aagagatcat ctacgggccg gaacatgttt ctaccggtgc gtccaacgat attaaagttg
    35821 cgaccaacct ggcacgtaac atggtgactc agtggggctt ctctgagaaa ttgggtccac
    35881 tgctgtacgc ggaagaagaa ggtgaagtat tcctcggccg tagcgtagcg aaagcgaaac
    35941 atatgtccga tgaaactgca cgtatcatcg accaggaagt gaaagcactg attgagcgta
    36001 actataatcg tgcgcgtcag cttctgaccg acaatatgga tattctgcat gcgatgaaag
    36061 atgctctcat gaaatatgag actatcgacg caccgcagat tgatgacctg atggcacgtc
    36121 gcgatgtacg tccgccagcg ggctgggaag aaccaggcgc ttctaacaat tctggcgaca
    36181 atggtagtcc aaaggctcct cgtccggttg atgaaccgcg tacgccgaac ccgggtaaca
    36241 ccatgtcaga gcagttaggc gacaagtaag ttcccgcatc agatgactgt atttgtaccg
    36301 aaaaccccgg ggcgtgctcc ggggtttttt cttatcaatt cataccaggg ataacataat
    36361 gaaactcttt gcccagggta cttcactgga ccttagccat cctcacgtaa tggggatcct
    36421 caacgtcacg cctgattcct tttcggatgg tggcacgcat aactcgctga tagatgcggt
    36481 gaaacatgcg aatctgatga tcaatgctgg cgcgacgatc attgacgttg gtggcgagtc
    36541 cacgcgccca ggggcggcgg aagttagcgt tgaagaagag ttgcaacgtg ttattcctgt
    36601 ggttgaggca attgctcaac gcttcgaagt ctggatctcg gtcgatacat ccaaaccaga
    36661 agtcatccgt gagtcagcga aagttggcgc tcacattatt aatgatatcc gctccctttc
    36721 cgaacctggc gctctggagg cggctgcaga aaccggttta ccggtttgtc tgatgcatat
    36781 gcagggaaat ccaaaaacca tgcaggaagc tccgaagtat gacgatgtct ttgcagaagt
    36841 gaatcgctac tttattgagc aaatagcacg ttgcgagcag gcgggtatcg caaaagagaa
    36901 attgttgctc gaccccggat tcggtttcgg taaaaatctc tcccataact attcattact
    36961 ggcgcgcctg gctgaatttc accatttcaa cctgccgctg ttggtgggta tgtcacgaaa
    37021 atcgatgatt gggcagctgc tgaacgtggg gccgtccgag cgcctgagcg gtagtctggc
    37081 ctgtgcggtc attgccgcaa tgcaaggcgc gcacatcatt cgtgttcatg acgtcaaaga
    37141 aaccgtagaa gcgatgcggg tggtggaagc cactctgtct gcaaaggaaa acaaacgcta
    37201 tgagtaatcg taaatatttc ggtaccgatg ggattcgtgg tcgtgtaggg gatgcgccga
    37261 tcacacctga ttttgtgctt aagctgggtt gggccgcggg taaagtgctg gcgcgccacg
    37321 gctcccgtaa gattattatt ggtaaagaca cgcgtatttc tggctatatg ctggagtcag
    37381 cactggaagc gggtctggcg gcagcgggcc tttccgcact cttcactggc ccgatgccaa
    37441 caccggccgt ggcttatctg acgcgtacct tccgcgcaga ggccggaatt gtgatatctg
    37501 catcgcataa cccgttctac gataatggga ttaaattctt ctctatcgac ggcactaaac
    37561 tgccggatgc ggtagaagag gccatcgaag cggaaatgga aaaggagatc agctgcgttg
    37621 attcggctga actgggtaaa gccagccgta tcgttgatgc cgcgggtcgc tatatcgagt
    37681 tttgcaaagc cacgttcccg aacgaactta gcctcagtga actgaagatt gtggtggatt
    37741 gtgcaaacgg tgcgacttat cacatcgcgc cgaacgtgct gcgcgaactg ggggcgaacg
    37801 ttatcgctat cggttgtgag ccaaacggtg taaacatcaa tgccgaagtg ggggctaccg
    37861 acgttcgcgc gctccaggct cgtgtgctgg ctgaaaaagc ggatctcggt attgccttcg
    37921 acggcgacgg cgatcgcgtg attatggttg accatgaagg caataaagtc gatggcgatc
    37981 agatcatgta tatcatcgcg cgtgaaggtc ttcgtcaggg ccagctgcgt ggtggtgctg
    38041 tgggtacatt gatgagcaac atggggcttg aactggcgct gaaacagtta ggaattccat
    38101 ttgcgcgcgc gaaagtgggt gaccgctacg tactggaaaa aatgcaggag aaaggctggc
    38161 gtatcggtgc agagaattcc ggtcatgtga tcctgctgga taaaaccact accggtgacg
    38221 gcatcgttgc tggcttgcag gtgctggcgg cgatggcacg taaccatatg agcctgcacg
    38281 acctttgcag cggcatgaaa atgttcccgc agattctggt taacgtacgt tacaccgcag
    38341 gtagcggcga tccgcttgag catgagtcag ttaaagccgt gaccgcagag gttgaagctg
    38401 cgctgggcaa ccgtggacgc gtgttgctgc gtaaatccgg caccgaaccg ttaattcgcg
    38461 tgatggtgga aggcgaagac gaagcgcagg tgactgaatt tgcacaccgc atcgccgatg
    38521 cagtaaaagc cgtttaaagc gttagataac tggctaaaaa ggcggcgatt gtcgcctttt
    38581 ttctcggcga acacatcggt tttgctgttt ttttccgcag ttgatacaat gcgataaaat
    38641 tgcccttgcg aaggtcattc gttttggtta gtattcacac ccgcttcagt gggaaagatt
    38701 aaaaactccc gctttattgg ttgaagcatt ggtacgcggc aactccgcaa ggaacaggtt
    38761 gattatgtac gaagctcttt tagtagtttt ccttattgtg gcaattggcc ttgttggtct
    38821 gatcatgctg cagcaaggta aaggcgctga tatgggagcc tccttcggag caggcgcttc
    38881 cgctacgctg tttggttcaa gtggttctgg taacttcatg acccgcatga cggcgctgct
    38941 ggcaacgtta ttcttcatca tcagtctggt gctgggtaac atcaatagca acaaaaccaa
    39001 tagaggtagc gaatgggaaa atctgagtgc accggcgaaa accgaacaaa ctcagccagc
    39061 tgctccggct aagccgacca gcgatatccc gaactaaaaa gtagtatccg tgccgaggtg
    39121 gtggaattgg tagacacgct accttgaggt ggtagtgccc aatagggctt acgggttcaa
    39181 gtcccgtcct cggtaccaaa ttccagaaaa gagacgctga aaagcgtctc ttttcgtttt
    39241 ggtcctggtc tgatgaatgg ttgtcaggta tctgtatctc gtccagttgc tttactcgta
    39301 gctggtacct tgaaacgatg gtgccggtac gccttagtta taaacttttc cctactgctg
    39361 acataattgg cactttattg tgtcaactcc agttccggta cgaatacgga tatgtttact
    39421 tctcgataaa ccatcctgct cctgtcagta taactactga tggctacgtt ctgcgtaatg
    39481 tttagagacg cattgataat gttatttaat tactgagaac gcaatggtcg catcatttaa
    39541 aaagaaaaaa ctaacagtgt ttcggtctga tatggatcag agttgaaaaa catgtctgat
    39601 ataaaattga acattgttga taattgttta tattggttgt gaacttggat ttctggatga
    39661 cagtattcaa caaatttgct agaagatttt aaatctcatt ggttgttgta tctttgtgtt
    39721 attgtttttg gtattacgaa cttagtcgcc tcttccggag cgcatatggt tcagcgattg
    39781 ctgttcttcg tcctgaccat cctggttgtt aaacgtatat catcccttcc gcttcgcctg
    39841 cttgttgccg caccatttgt gttactgact gcggcagaca tgagtattag cctctattca
    39901 tggtgtacct ttggtacaac tttcaatgat ggatttgcga ttagtgtgct gcagagtgat
    39961 ccggatgaag ttgtgaaaat gctggggatg tatatccctt atctatgtgc ttttgctttt
    40021 ttatcccttc tttttttggc agtaataata aaatatgatg tttccttgcc gacaaaaaaa
    40081 gtgacaggaa tattattgct gattgtcatt tcgggcagtt tattttccgc ttgtcaattt
    40141 gcttataaag atgcaaaaaa taaaaaagcg ttcagtccat atatactagc gtcgtgattt
    40201 gctacctata cgccgttttt caatctcaac tattttgctt tagcagcgaa agcgcatcaa
    40261 agattactct caattgcaaa cacggtgccg tattttcaat tatcagtcag ggatacaagt
    40321 attgatacct acgtgttgat tgtgggggag tctgtacgtg tcgacaatat gtctttgtat
    40381 ggatatacac gctctacgac accgcaagtt gaagcacaaa gaaaacagat caaactgttt
    40441 aatcaagcaa taagcggcgc accttacact gcgctgtcgg ttcccctttc tttaactgct
    40501 gattctgttt tgagtcatga cattcataat taccccgaca acattattaa tatggctaat
    40561 caagcaggat ttcagacttt ctggctaagc tcgcaatccg cttttcggca gaatggtaca
    40621 gcagttacca gtatcgccat gcgcgccatg gaaacagttt atgtcagagg atttgatgaa
    40681 ttgttgttgc cgcatttatc gcaagcatta cagcaaaata cgcagcaaaa gaaactgatt
    40741 gttcttcatt taaatggaag ccatgaaccg gcttgtagcg cctatccgca atccagcgcc
    40801 gtgtttcaac cgcaggacga tcaggatgcc tgctatgaca actccattca ttacacagat
    40861 agtttgctag gtcaggtttt tgaattatta aaagatcgcc gcgcctcggt catgtatttt
    40921 gccgaccacg gcctggaacg tgaccctacg aagaagaacg tctattttca tggaggcagg
    40981 gaggctagcc agcaggcata tcatgtcccg atgtttatct ggtatagccc cgttcttggg
    41041 gatggcgtgg atcgcacaac ggaaaacaat atcttttcga cagcttacaa taattacctt
    41101 attaatgcgt ggatgggggt aacaaagccg gaacagccgc aaacgcttga ggaagtgatt
    41161 gcacactata aaggagactc acgggttgtg gatgcaaacc atgatgtttt cgattatgtg
    41221 atgctcagaa aggagtttac agaggataag caaggtaacc ccacccctga agggcagggt
    41281 tgatgtcgaa ttactggcct ttgttttcca tattctccac ctgcggcagg cctgaagtgg
    41341 cagaggagga aagcaggcca gttttggcat aaccaaaaag tttctctcgg gtatcagtga
    41401 tatccagatt acgcatggtc aattgaccaa tacgatcatc tggcgagaac accgagtcgc
    41461 ctttttccat cgtcagacgc tctggcttgt aggtcaggtt ctctgagacg gtattcagga
    41521 ttgaataatc gttcccacgg cgcagctcca gggtaacttc accagtgatc tggctggcaa
    41581 cccagcgttg cagagagtca cgcagcatca gcgcctggga gtcaaaccaa cgcccctggt
    41641 acagcagacg gcccaactga cgaccatgcg cgtgatactg ctcaatggtg tcttcgttgt
    41701 gaataccggt caacagacgt tcatacgcaa tgtgcagcag tgccatcccc ggagcttcgt
    41761 aaataccacg gcttttcgct tcgatgatac ggttctcaat ctggtcgctc atgcccaggc
    41821 cgtgacgacc gccgatgcgg ttagcttcca gcatcatttc tacgtcgtcg ctaaaggttt
    41881 taccgttcag cgccaccgga tgaccttgct caaagcgtac tgtgacttct tctgccggga
    41941 tcttcacgct ctcatcccag aatttcacgc ccataatcgg gttgacgatt ttgacgctgg
    42001 agttgaggta ttccagatcc ttcgcttcat gcgttgcacc aagcatgttg gagtctgtgg
    42061 agtaggcttt ttcgacagac attttgtagt cgaaaccgca ggcaatcata aattcagaca
    42121 tctcatgacg gccgcccagt tcatcaataa agtcagtatc aagccacggt ttgtaaatct
    42181 gcagttcagc attggtcagc agaccataac gatagaaacg ttcgatatcg tttcctttat
    42241 aggtgctacc gtcaccccag atattcacgc catcttcttt catcgcagca accagcatgg
    42301 taccagtcac ggcgcggccc agcggcgtcg tgttgaaata ggtcaggccg ccggtggtgt
    42361 tatgaaatgc gccacactga atagcggcaa taccttcggc caccagttgt ttgcggcagt
    42421 cgatcagacg tgcgttctcc gcgccgtatt ccatggcacg acgagggatc gcatcataat
    42481 cctcttcgtc tggctggccc aggtttgcag tatatgcata aggaaccgct cccttttgtc
    42541 gcatccacag cagtgcggca ctggtgtcca gaccgccgga aaaagcgata ccaatacgtt
    42601 gacctaccgg gagatgcttg agaatcgtcg tcataaaata acaccctgct taattaactg
    42661 atgatgagcc tggatttccg ctctcactga atttttatgc aaaataaatg agttttcatt
    42721 taatcatctt ttatcggaga caggaagagt ttagtgtgtt ttttgtaaaa taatgcgctt
    42781 aagggagagc aggggaaggc aaaagtattc aacaaatgaa agtgaactgg atattcattc
    42841 acgtgattag caataaacgt tgacaaaatg tggcatggat cactataatg cctgcagatt
    42901 ttacgtcccg tctcggtaca ccaaatccca gcagtatttg cattttttac ccaaaacgag
    42961 tagaatttgc cacgtttcag
//
LOCUS       DADLTY010000008        42932 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.008, whole genome shotgun sequence.
ACCESSION   DADLTY010000008 DADLTY010000000
VERSION     DADLTY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.008"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(63..1193)
                     /gene="dnaJ"
                     /locus_tag="JN334_000387"
     CDS             complement(63..1193)
                     /gene="dnaJ"
                     /locus_tag="JN334_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAY7436655.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1282..3198)
                     /gene="dnaK"
                     /locus_tag="JN334_000388"
     CDS             complement(1282..3198)
                     /gene="dnaK"
                     /locus_tag="JN334_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAY7436656.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3575..3979
                     /locus_tag="JN334_000389"
     CDS             3575..3979
                     /locus_tag="JN334_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAY7436657.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4005..4718
                     /gene="msyB"
                     /locus_tag="JN334_000390"
     CDS             4005..4718
                     /gene="msyB"
                     /locus_tag="JN334_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAY7436658.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            4867..5433
                     /gene="satP"
                     /locus_tag="JN334_000391"
     CDS             4867..5433
                     /gene="satP"
                     /locus_tag="JN334_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HAY7436659.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5468..6055)
                     /gene="mog"
                     /locus_tag="JN334_000392"
     CDS             complement(5468..6055)
                     /gene="mog"
                     /locus_tag="JN334_000392"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HAY7436660.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6170..7123)
                     /gene="tal"
                     /locus_tag="JN334_000393"
     CDS             complement(6170..7123)
                     /gene="tal"
                     /locus_tag="JN334_000393"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY7436661.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7402..8832
                     /locus_tag="JN334_000394"
                     /pseudo
     CDS             7402..8832
                     /locus_tag="JN334_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            8902..9678
                     /gene="yaaA"
                     /locus_tag="JN334_000395"
     CDS             8902..9678
                     /gene="yaaA"
                     /locus_tag="JN334_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAY7436662.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(9831..10127)
                     /locus_tag="JN334_000396"
     CDS             complement(9831..10127)
                     /locus_tag="JN334_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY7436663.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10341..11627)
                     /gene="thrC"
                     /locus_tag="JN334_000397"
     CDS             complement(10341..11627)
                     /gene="thrC"
                     /locus_tag="JN334_000397"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAY7436664.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(11628..12560)
                     /gene="thrB"
                     /locus_tag="JN334_000398"
     CDS             complement(11628..12560)
                     /gene="thrB"
                     /locus_tag="JN334_000398"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAY7436665.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12562..15024)
                     /gene="thrA"
                     /locus_tag="JN334_000399"
     CDS             complement(12562..15024)
                     /gene="thrA"
                     /locus_tag="JN334_000399"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HAY7436666.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(15105..15170)
                     /gene="thrL"
                     /locus_tag="JN334_000400"
     CDS             complement(15105..15170)
                     /gene="thrL"
                     /locus_tag="JN334_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HAY7436667.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15384..16070)
                     /locus_tag="JN334_000401"
     CDS             complement(15384..16070)
                     /locus_tag="JN334_000401"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY7436668.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16470..16610)
                     /locus_tag="JN334_000402"
     CDS             complement(16470..16610)
                     /locus_tag="JN334_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436669.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            16706..17422
                     /gene="arcA"
                     /locus_tag="JN334_000403"
     CDS             16706..17422
                     /gene="arcA"
                     /locus_tag="JN334_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HAY7436670.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17481..18833)
                     /gene="creD"
                     /locus_tag="JN334_000404"
     CDS             complement(17481..18833)
                     /gene="creD"
                     /locus_tag="JN334_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HAY7436671.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(18891..20315)
                     /gene="creC"
                     /locus_tag="JN334_000405"
     CDS             complement(18891..20315)
                     /gene="creC"
                     /locus_tag="JN334_000405"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HAY7436672.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20315..21004)
                     /gene="creB"
                     /locus_tag="JN334_000406"
     CDS             complement(20315..21004)
                     /gene="creB"
                     /locus_tag="JN334_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HAY7436673.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21017..21490)
                     /gene="creA"
                     /locus_tag="JN334_000407"
     CDS             complement(21017..21490)
                     /gene="creA"
                     /locus_tag="JN334_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HAY7436674.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            21701..22570
                     /gene="robA"
                     /locus_tag="JN334_000408"
     CDS             21701..22570
                     /gene="robA"
                     /locus_tag="JN334_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HAY7436675.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(22567..23214)
                     /gene="gpmB"
                     /locus_tag="JN334_000409"
     CDS             complement(22567..23214)
                     /gene="gpmB"
                     /locus_tag="JN334_000409"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HAY7436676.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23266..23786
                     /gene="yjjX"
                     /locus_tag="JN334_000410"
                     /pseudo
     CDS             23266..23786
                     /gene="yjjX"
                     /locus_tag="JN334_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
     gene            complement(23871..24197)
                     /gene="trpR"
                     /locus_tag="JN334_000411"
     CDS             complement(23871..24197)
                     /gene="trpR"
                     /locus_tag="JN334_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HAY7436677.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24287..26224)
                     /gene="sltY"
                     /locus_tag="JN334_000412"
     CDS             complement(24287..26224)
                     /gene="sltY"
                     /locus_tag="JN334_000412"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAY7436678.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26435..28102
                     /gene="ettA"
                     /locus_tag="JN334_000413"
     CDS             26435..28102
                     /gene="ettA"
                     /locus_tag="JN334_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAY7436679.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28410..29642)
                     /gene="nadR"
                     /locus_tag="JN334_000414"
     CDS             complement(28410..29642)
                     /gene="nadR"
                     /locus_tag="JN334_000414"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HAY7436680.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(29663..31045)
                     /gene="radA"
                     /locus_tag="JN334_000415"
     CDS             complement(29663..31045)
                     /gene="radA"
                     /locus_tag="JN334_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAY7436681.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31094..32062)
                     /gene="serB"
                     /locus_tag="JN334_000416"
     CDS             complement(31094..32062)
                     /gene="serB"
                     /locus_tag="JN334_000416"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HAY7436682.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32168..32812
                     /locus_tag="JN334_000417"
     CDS             32168..32812
                     /locus_tag="JN334_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HAY7436683.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            32840..33855
                     /gene="lplA"
                     /locus_tag="JN334_000418"
                     /pseudo
     CDS             32840..33855
                     /gene="lplA"
                     /locus_tag="JN334_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            33887..34150
                     /locus_tag="JN334_000419"
     CDS             33887..34150
                     /locus_tag="JN334_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7436684.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(34311..35030)
                     /gene="deoD"
                     /locus_tag="JN334_000420"
     CDS             complement(34311..35030)
                     /gene="deoD"
                     /locus_tag="JN334_000420"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAY7436685.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35087..36310)
                     /gene="deoB"
                     /locus_tag="JN334_000421"
     CDS             complement(35087..36310)
                     /gene="deoB"
                     /locus_tag="JN334_000421"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY7436686.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(36362..37684)
                     /gene="deoA"
                     /locus_tag="JN334_000422"
     CDS             complement(36362..37684)
                     /gene="deoA"
                     /locus_tag="JN334_000422"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HAY7436687.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(37811..38590)
                     /gene="deoC"
                     /locus_tag="JN334_000423"
     CDS             complement(37811..38590)
                     /gene="deoC"
                     /locus_tag="JN334_000423"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAY7436688.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            38848..40389
                     /locus_tag="JN334_000424"
     CDS             38848..40389
                     /locus_tag="JN334_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HAY7436689.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            40361..41224
                     /gene="yjjW"
                     /locus_tag="JN334_000425"
     CDS             40361..41224
                     /gene="yjjW"
                     /locus_tag="JN334_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HAY7436690.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(41438..42220)
                     /locus_tag="JN334_000426"
     CDS             complement(41438..42220)
                     /locus_tag="JN334_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7436691.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(42217..>42932)
                     /locus_tag="JN334_000427"
     CDS             complement(42217..>42932)
                     /locus_tag="JN334_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAY7436692.1"
                     /translation="MDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVIRASS
                     AIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQWFKR
                     MERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIALGSRI
                     PALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVANDTLVA
                     EVSDAPQANDPTFNNEDLA"
BASE COUNT        10070 a        11730 c        10801 g        10331 t
ORIGIN      
        1 cgcatatctt caacgcaccc tatttttacc caggcctgcc cacgggcagg cttttgggga
       61 ggttagcggg tcaggtcgtc aaaaaacttc ttcacaccat caaagaagct ctttgagcgc
      121 gggctgttgt gctcgccggt tgggccaccg aagctttctt gcagctcttg cagcagctgt
      181 ttctgctttt cgttcaggcc taccggtgtt tcgacgacaa cgcggcacag caaatcaccc
      241 tgtgcgccac cgcggacaga cttgacgcct ttaccgcgca tacggaatag cttaccggtc
      301 tgggtttcgc caggcacttt cagtttgacg cgaccatcaa gggtcggtac ttcgatttcg
      361 ccacccagcg ccgccatagc gaagttgatc gggacttcgc aatacaggtt gttgccttca
      421 cgctcgaaaa tcgggtgctg tttaacctga acctgaacgt acagatcgcc tgccggtgcg
      481 ccatgctcgc ccgcttcacc ttcgcccgca agacggatgc ggtctccagt gtccacccct
      541 gccgggattt taacggacag cgttttgctg cgctcaacac gaccatgacc atgacatttg
      601 ttgcacggat ctttgatcag cgtaccgcgg ccctgacagt gtggacaggt ctgctgtaca
      661 gcgaagaatc cctggcgcat ctgcacctga ccagaaccat gacaggtcgg acaggtctgc
      721 ggctgtgtac ctggttttgc accgctaccg tggcaaacgt cacactcttc cagagtcgga
      781 atgcggatct ctttggtcac gccacgtaca gcttcttcga gggtgagctc catgttatag
      841 cgtaaatcag caccgcgcgc cgcacgttga cgaccacgtc cgccgccaaa aatatcgccg
      901 aaaacgtcac caaaaatatc gctgaagtct gcgccgccgc caaaaccacc gccgcccatg
      961 cctccttgct caaacgcagc atgaccatac tgatcgtatg ccgcacgttt ttgcgagtcg
     1021 gtcagaactt cataagcttc cttgatctct ttaaatttcg cctcggcctc tttgtcaccc
     1081 tggttacggt ccgggtggta tttcatggcc aggcgtttgt aggcctttct gatttcacgc
     1141 tcttccgctg ttttggaaac gcctaaaatc tcgtaataat cttgcttagc catctttttt
     1201 aaattgcccc tagatgaatg cacgggcgga gaggaaattc cccttcgccc gtgtcagtat
     1261 aattacccgt ttatagggcg attatttttt gtctttgact tcttcaaatt cagcgtcgac
     1321 aacatcgtca tctttcgcgt tgtttgcaga agcatcagca ccggcagtct gctgctgggc
     1381 atgttgctgc tgggcgattt ccatcagttt ctgggaaacc tgtgccagtt cctgcatttt
     1441 cgcttcgata gcggctttgt cttcaccttt cagagcagtt tccagtgcag tcagcgcaga
     1501 ctcgatagca gttttgtcgt cagccggcag tttgtcgcct gcttcttcaa cctgcttacg
     1561 ggtgctgtgc agcagatggt cgccctggtt gcgagtctgt accagctctt caaacttacg
     1621 gtcagcttcg gcgttagctt ctgcgtcgcg taccattttc tggatttcat cttcgttcag
     1681 accagaagaa gccttgatgg tgatcttctg ctctttaccg ctgtttttat ctttcgcgga
     1741 aacgtgcagg ataccgtcag catcgatatc gaaggtaact tcgatctgcg gcatgccgcg
     1801 cggtgccggg ttgataccat ctaggttgaa ctgacccaga gatttgttat cagccgcacg
     1861 tttacgttca ccctgcagca catggatggt taccgcagac tggttgtctt cagcggtaga
     1921 gaacacctgg ctgtgcttgg tcgggatagt ggtgtttttc gcgatcagcg tcgtcatcac
     1981 accgcccatg gtttcgatac ccagagacag cggggtaacg tccagcagca gtacgtcttt
     2041 tacgtcacca gtcagaacac caccctgaac agcagcaccg attgctacag cttcgtccgg
     2101 gttaacgtct ttacgcggct ctttaccaaa gaactcagca actttcttct gaaccattgg
     2161 catacgagtc tgaccaccaa cgaggataac gtcgtcgata tcagatacgg acaggccagc
     2221 atcctgcagt gcaactttca gcggctcaat ggaacggttt accagatctt caaccaggct
     2281 ttccagtttc gcacgagtca ctttgatgtt catgtgtttc ggaccggtcg cgtctgcagt
     2341 gatgtatggc aggttaacgt cggtctgctg agcggaagac agttcgattt tcgctttttc
     2401 tgccgcttct ttcaggcgct gcattgccag cggatcgttg cgcaggtcaa tgccctgatc
     2461 tttcttgaat tcttcaacca gatagttgat cagacggctg tcgaagtctt caccacccag
     2521 gtgggtatca ccgttggttg ccagaacttc gaaggttttt tcgccgtcaa cttcgtcgat
     2581 ttcgataata gaaatatcga aagtaccacc ccccaggtca taaaccgcga tagtacggtt
     2641 gccagtgcct ttgtccagac cgtaagccag cgcagctgcg gtcggttcgt tgatgatacg
     2701 ttttacttcc agaccagcga tacggcctgc gtctttggtt gcctgacgct gagcatcgtt
     2761 aaagtatgcc ggtacggtga taacagcttc agttaccggt tcacccaggt aatcttcagc
     2821 ggttttcttc atttttttca gcacttcagc agaaatctgc ggcggtgcca ttttctggcc
     2881 tttaacttcg acccatgcgt cgccgttatc agcagcaata attttgaacg gcatgatgga
     2941 aacatcacgc tgtacttctt cgtcctggaa gcggcggcca atcaggcgtt taatcgcaaa
     3001 cagggtgttt tgcgggttcg tcactgcctg acgtttagcc ggctgaccaa ccagagtttc
     3061 accatcctgg gtataggcaa tgatagaagg cgtggtgcga tcgccttcgg cgttctccag
     3121 cacgcgagga atggtgccat ccataatcgc tacacaagag ttggtagtac ccaggtcgat
     3181 accaattatt ttacccatct aaacgtctcc actatatatt cggtcatcat gtggttgtga
     3241 gtctgtaata ggggcgaaac gtctggtttc aactgcccaa tttcattttt ttgcagactc
     3301 actcactgcg gttgactact aaatggggtc gtaacccacg tcatcaaggg ggagatgcaa
     3361 aaattttttg tgcttttgcg cagaaatttc gcgatatggc aggacaagac gtaaaattga
     3421 cagcaatttt attcatataa agaatgaatc gcagccaagg cgttcatcaa aaaattgtaa
     3481 taaaaagaaa agattacgtg cctgaatctt ctctttatca gcagtaaact agtgggtatt
     3541 catcccccta cctcttccca ctaagagaat ccttatgaaa tccgttttta cgatttccgc
     3601 cagcctggcg attagcctga tgctgtgctg cacggcgcag gcaaacgacc ataaactcct
     3661 cggcgtcatt gcaatgccgc gtaacgaaac caacgatctg gcgctgaaac ttcctgtttg
     3721 tcgcattgtg aaacgcatac aactctctgc cgaccatggc gatttacagt taagtggtgc
     3781 atcggtttat ttcaaagccg cccgtagcgc cagtcagagc ctgaatattc cttcagaaat
     3841 aaaagaaggg caaaccactg actggatcaa cattaacagc gataacgaca ataaacgctg
     3901 cgtctcaaaa atcacctttt ccggtcatac ggtgaactca tcggatatgg ccacgctgaa
     3961 aattatcggc gacgactaac gccagaatat agaagccaca aaaaatgaat gttaattacc
     4021 tgaatgattc agatctggat tttcttcagc attgtagtga ggaacagttg gcaaatttcg
     4081 cccgattgct cacccataat gaaaaaggca aaactcgcct ctccagcata ctgatgcgca
     4141 acgaactgtt taaatcgatg gaagggcatc ccgagcaaca tcgccgcaac tggcagctga
     4201 ttgccggaga attacagcat tttggtggcg atagtatcgc caacaaactg cgcggacacg
     4261 gtaaattgta tcgggccatt ttgctcgatg tttcaaagcg attgaagctg aaagccgaca
     4321 aagagatgtc tacgtttgaa attgagcaac agttactgga acaatttctg cgtaatacct
     4381 ggaagaaaat ggacgaggaa cataagcagg agtttctgca cgcggtcgat gccagggtga
     4441 atgagctgga agagctgctg ccgctgctga tgaaagacaa attattggca aaaggtgtgt
     4501 cgcatttgct ttccagccaa ctgacccgca ttttacgcac ccacgcagca atgagcgtac
     4561 ttgggcatgg tttgctgcgc ggcgcggggc tgggaggccc tgtgggcgca gcattaaatg
     4621 gggttaaagc ggtcagcggc agcacctatc gcgtgacgat tccagccgta ctgcaaatcg
     4681 cctgcctgcg ccggatggtt agcgccactc aggtctgata ccggaaaccg gggaaaattt
     4741 tcattttccc ctcaaaagat cgtagacact gccccactgg ctgattatta tgccgcgccc
     4801 tgaaaacact acagttattc agggaaatta tttcaccatt cattcgatga tgatttttga
     4861 ggaattatgg gcaacactaa gttggctaat ccggcaccgc tgggcctgat gggcttcggc
     4921 atgaccacca ttctgcttaa cctgcacaac gtgggttatt tcgctctgga cggtattatt
     4981 cttgccatgg gcattttcta cggcggcatc gcgcaaattt ttgctggtct gctggagtac
     5041 aaaaaaggca acactttcgg tttaaccgca ttcacctctt acggttcttt ctggctgacg
     5101 ctggttgcga ttctgctgat gccgaaactg ggtctgaccg atgcgccaaa tgcacagttc
     5161 cttggtgtct acctgggtct gtggggcgta tttacgctgt ttatgttctt cggcactctg
     5221 aaaggcgcgc gcgtcctgca attcgttttc tttagcctga ccgtgctgtt tgccctgctg
     5281 gcgatcggta acattgccgg taatgccgcg atcatccact ttgccggctg gattggtctg
     5341 atctgcggtg ccagcgcaat ctatctggcg atgggtgaag tgctgaacga gcagtttggt
     5401 cgcaccgttc tgccgattgg tgaatcccac taattgtctt gagatccccc cgctcggggg
     5461 gattttttta ttcgctaacg tcgcgtcttg cactcttcgg tctgaatgca gcaaccactt
     5521 ctggtgccgt ttcaacgtat ggcccttcca gcaactgaat gcagtacggt acgctggcaa
     5581 aaataccatg caccacaacg ttaccctcag cgtccttcac accttccagc gtctctttaa
     5641 tagacttcgg ctgaccgggt aagttaagga tcagcgcctg tttgcgaatc acgcccacct
     5701 gacgcgaaag gatcgcagtt ggtacaaaat gcaggctgat ctggcgcatc tgttcaccaa
     5761 agccaggcat ctcgcggtcc gctactgcca gcgtcgcatc gggcgttacg tcacgacgcg
     5821 ccgggccagt tccgcccgtg gtgagcacca gatggcaact catttcatcc accagctcac
     5881 acaacgtttg ctcgatgatc gcctgctcat cggggattaa gcgggtttcc agttcaaacg
     5941 gcgtggttag cgccgatgtc agccattctt ccagcgctgg aatgccttta tcctgataaa
     6001 cgccgctgga tgcgcgatca gagatggaaa ctaagccaat acgtaaagta ttcatcaaga
     6061 tttttccgtt taaacaatct cgttaaacag aatgatacac tgcgaaggga gtgacagaca
     6121 ggcttcagaa gaggtagcgt gaccgacttc ccggtcacgc taagaatgat tacagcagat
     6181 cgccgatcat tttttccagt ttttcctggt caatagcaaa cttacggata ccttccgcca
     6241 gtttatctac tgccattgga tcctggttgt gctgccacag gaactcggac tcagtgatac
     6301 gcgccggacg cgctttcact tcgccggtgt aagacagttt acgttcgata gccccttcgc
     6361 tctccgccag ctctttcagc agtgccggtg cgatggtcag acggtcacag cctgccagtt
     6421 ccagaatttc gccgatgtta cggaagcttg cgcccataac cacggtttca taaccgtgct
     6481 ctttgtagta ctggtagatt tcagatacag aaaccacgcc cggatcttct gccggagcgt
     6541 actctttctt atcggtattc gctttgtacc agtcaagaat acggccaaca aacggcgaga
     6601 tcaggaacac gcccgcctcc gcacaagcac gagcctgagc gaaggagaac agcagggtca
     6661 ggttacagtt gataccttct ttttccagct gttctgcagc acggataccc tgccaggtag
     6721 aagccagttt gatcagaata cgatcgttgc taatacctgc atcgttgtag agtttgatca
     6781 ggcgttttgc tttcgcaatt gacgcttcag tgtcatagga aagacgcgca tcaacttcag
     6841 ttgagatacg gcccggaacc agtttcagga tttccagacc aatatttact gccagtttgt
     6901 cggtcgcgtc cacgatctgc tgcgcgcgat cgttgctctg ctgtttcgcc caggcgacag
     6961 catcatcaat caacttacgg tattccggaa tctgcgctgc gttaagaatg agagaagggt
     7021 tggttgtggc atcctgcggt tgatacagct tcattgccgc gatgtcccca gtgtcggcca
     7081 ctacggtggt gtactgacga agggaggtca atttgtccgt catgatagta tttctcttta
     7141 aacagcttgt tagggggatg taaccggtct gccctgatga tatcacgacg caacgtcagc
     7201 gcaaccgccg acaagacggt tatcgccagg cgagactgtt tcggatttct gacactgcgt
     7261 tgatatcgcc caccattttt atacaaaacc tcatgtatgc tacgcagaag ttatcaagta
     7321 cctcgtagcg tatatacttc ttaaacaatt ggtaacgttt acacaggaaa gtcatcgcga
     7381 ccggcaataa gagggatatg catgccagat tttttctcct ttattaacag cgtcctttgg
     7441 ggatcggtaa tgatttacct gctcttcggc gcaggttgtt ggttcacttt tcgcaccgga
     7501 tttgtgcagt ttcgctacat ccgccagttt ggcaaaagtc ttaaaaatag cattcatcca
     7561 cagccaggcg gtttaacctc atttcagtca ttgtgtacca gtcttgcggc gcgcgtgggt
     7621 agcggcaacc tggccggcgt tgcgctggca attaccgccg gtggaccagg tgccgtcttt
     7681 tggatgtggg ttgctgcgtt tatcggcatg gcgacctcgt ttgccgaatg ttcccttgca
     7741 caactttata aagaacgtga cgtcaatggg cagtttcgtg gcggaccggc atggtatatg
     7801 gcgcgcgggc tggggatgcg ctggatgggc gttctgttcg ccgtcttttt gctcatcgcc
     7861 tacggcataa ttttcagcgg agttcaggcg aacgccgttg cccgagccct gagtttttct
     7921 tttgattttc ctccgctggt gacgggcatt attctcgctg tctttgctct gctggcaatc
     7981 actcgtggtc ttcatggcgt cgcccggctc atgcaggggt ttgtcccgtt gatggcgata
     8041 atctgggtac tgaccagcct ggtgatttgc gtaataaata tcgggcaact tccccacgtc
     8101 atttggtcta tttttgagag tgcttttggc tggcaggaag cggcaggcgg cgcggcggga
     8161 tataccttaa gccaggcgat tactaacggt tttcagcgca gtatgttttc caatgaggtg
     8221 ggaatgggat cgacgccaaa cgcggcagcg gcagctgcat cctggcctcc gcatccggca
     8281 gcacaaggga ttgtccagat gattggcatt tttatcgaca ccctggtcat ctgtacggca
     8341 agcgccatgc tgatattact ggcgggtaac ggcacaacct acatgccgct ggaaggtatt
     8401 cagcttatcc agaaggcgat gcgggtgcta atgggttcct agggtgctga atttgttacc
     8461 ctcgtggtta ttctgtttgc cttcagctcc atcgttgcca actacattta tgccgaaaac
     8521 aatctcttct ttttacgcct gaacaaccct aaagcgatct ggtgtttgcg gatctgcacc
     8581 ttcgcaacgg tcatcggcgg caccttgcta agccttccgc tgatgtggca actggcagat
     8641 atcataatgg cctgcatggc tattaccaat ttgaccgcca ttttactgct ctcgcctgtg
     8701 gttcatacca ttgccagtga ttatctacgc cagcgtaaac tcggcgtgcg cccggtgttt
     8761 gatccgttgc gttatccgga tatcggtcgc cagctttctc cggacgcgtg ggatgatgtt
     8821 tcgcaggagt aatcacaact atcgatcaac tcattctctt tttttgctaa agtcggcata
     8881 aatttcctgc aaggactgga tatgctgatt cttatttcac ctgcgaaaac gcttgattac
     8941 caaagcccgt tgaccaccac gcgctatacg ctgccggagc tgttagacaa ttcccagcag
     9001 ttgatccatg aagcgcggaa actgacgcct ccgcagatta gcacgctgat gcgcatcagc
     9061 gacaaactgg cgggtatcaa cgccgctcgc tttcatgact ggcagccaga tttcacgccg
     9121 gcgaatgccc gccaggcgat tctggcgttt aaaggtgatg tctacaccgg cttgcaggcc
     9181 gaaaccttca gcgaagacga tttcgatttt gcccaacagc atttgcgaat gctttccggc
     9241 ttgtatggcg tactgcgccc gctcgattta atgcagcctt atcgtctgga aatggggatc
     9301 cgtcttgaga atgcccgagg gaaagatctg tatcaattct ggggagatat catcaccaac
     9361 aagctgaacg aggcgctcgc agcacaaggc gataatgtgg tgattaatct ggcgtcagat
     9421 gagtatttta aatcggtgaa gccgaagaaa ttgaatgccg agattatcaa gccggtgttc
     9481 ctcgatgaga agaacggcaa atttaagatc atcagcttct acgctaagaa agcacgcggt
     9541 ctgatgagtc gtttcattat tgaaaatcgg ctgaccaaac cagagcaact gactggtttt
     9601 aatagcgaag gttacttctt tgatgaagat tcctccagca atggcgaact ggtgtttaaa
     9661 cgctacgagc agcgttaagt ttgtagtcaa tgccggatgc ggcgtgaacg ccttatccgg
     9721 cctacaaaag catgcagatt caatatattg cagaattaac gtaggcctga taagacgcgc
     9781 cagcgtcgca tcaggcgctg aatgccggat tgttacccgg catttgtcat ttagcggtga
     9841 tgtttgcctg gaccatgacc gccgtgatga tcatgaggag ctttcttatg gtggcgcggc
     9901 ggtggcggcg gtccgtgtgg gtgccagcga ttgcctcgcc attcataatg ttgtttccac
     9961 cagccgtggt cgcgccagtg acctccatcc cagtaatagc cacgattatc acgatcgcct
    10021 atctgtaatt ttactgacgg gactaacgta atttccgcag cctgtgctgc catgggagcg
    10081 accagaacca gggaaagtgc gagtacgata gattgcatct ttttcacagg ttactccttt
    10141 tatcattgcc cgttatgggt cgatatccgg agattacgcg atgagaacga cggttgttat
    10201 tctccgcaat cctaatctct aagttaccgc atttattgat aatttctcct ttttccctgt
    10261 cgtttttctc cataatttct tcataaaaaa gccgggctgc ataaaagcaa acccggcctg
    10321 attgagataa tgaatagatt ttactgatga ttcatcatca atttacgcaa cgcagaaaaa
    10381 tcggcgggca gattatgtga aagcaagggt aaatcagcac gttctgccag ctcttttggc
    10441 agatccaacg tttcaccgag aatcgcttcc acgctctctt taaatttcgc cggatgcgcg
    10501 gtgccgagga acaagccata ttcgcctgga ttcaactggt cacgcagcgc acgatacgct
    10561 acggcagcgt gcggctccga ggtgtagccc agttctttta actcacgcat tgtctgttgc
    10621 gtggtttcat catccacggc tgcataaccc agctctttca gttgccagat tttgcggcgg
    10681 aacaactctt ccacacgcgg ccagttgttc ggctgactca cgtccatcgc gttggataac
    10741 gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca ggaaacgtgg cacggtatcg
    10801 ttcacgttgg tcgcagcaat aaaacgtttc accggcagac cgaatgactt cgccagcaga
    10861 cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga caaccagctg gttgcgcgcc
    10921 tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa tctgcgccag caaacggctg
    10981 atgttaatcg agttagccga gtttaacccc agcgccactt tcagttcttc atcatcaaac
    11041 gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt cgatggcaac agtttcgata
    11101 ttgccgccca acgtacagaa cagtttttct tgcagtggac tgattttgcc tcgtggatag
    11161 aggataacca ctttcacatt cggcaaaccg tagaaagcat gagccactgc agctccggta
    11221 tcaccggagg tcgcggtcag aatggtcact ggcttatcat ccgcaatatg ggtcagcatt
    11281 tgtgccataa agcgaccgcc gaaatcttta aatgccagcg ttggcccgtg gaacaattcc
    11341 agacaaccga catcgctttc aacattggcg accggagccg ggaaggcaaa cgccgcgcgc
    11401 acgcgctctt ccaggatttc ctgtgggatt tcatcaccaa taaacgccga gaggatcttc
    11461 gcactgcggg tgacaaaatc cagcttcagc atctcatcaa tttcagtcag gctgaattcc
    11521 ggcaggtcgt gcggaaaaaa cagcccctga tttttgccca acccctgggt tacggcttgc
    11581 gcaaagctga cctgctcgtt gtgatctttc agattgtaga gtttcattta gttttccagt
    11641 actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa aaccttcctg attttgcagg
    11701 tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat ccggcttgtc acatagagcg
    11761 aacaaggtcg ggccggagcc ggagataccg ctcgctaccg cgccgatttc cgcgaccgcc
    11821 tgccgcgcct gccggaagcc aggcagtaac cgttcacggt agggttcagc gataacatct
    11881 ttcatcagct tcgcggcaag ctcaggctga cgggaatagc aggcgtgaat gaagccagcc
    11941 agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact gcgccggtaa aatagcccgg
    12001 gcttctgccg tcgagacttt aattcccgga tacgccagca cccacagcca ctcatcaaac
    12061 cctggcactt gctggctgat gatgtcgttt tcttcgatca tcaactgcat accaccaaga
    12121 aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga tacgtccttc cagctcgccc
    12181 atcaaagcca gcaaacgagt gtcattaagc ggcttgccgc agtgttcatt catcgccatc
    12241 agcgccgcga ccaccgaaca ggcgctggag cctaagcccg aaccgatcgg catattcttt
    12301 tccagagtca tcgccactgg aatttgcttg ccaagctcct ggcaaaaacg ctcccagcac
    12361 tgataaacga tattttcccg tggttctgac ggcagcttat cggcaaagcg tccgagtttg
    12421 ttgagactga atgtctctgc cgcctcaacc gtgactacat ctccgagcaa tgcaccatca
    12481 acgggtgcca ccgccgcccc gagcacatca aacccgacgc tcatattggc actggaagcc
    12541 ggggcataaa ctttaaccat gtcagactcc taacttccat gagagggtac gtagcagatc
    12601 ggcaaagaca ccggcagcgg taacgtcatt gcccgcacca tatccgcgca gcaccaacgg
    12661 cagcggctga taatagtggc tataaaaggc cagggcgttt tcgccatttt tcactttgaa
    12721 cagcggatca ttcccatcca cttcggcaat cttcacgcgg cagacaccat cttcatcaat
    12781 attgccaaca tagcgcaaaa cttttccttc atcacgggcc ttcgccacgc gcgcggcaaa
    12841 gagatcgtcg agctgtgaca gattcgccat aaaagcggca acatcaccct cggcgttaaa
    12901 ctctgcgggc agcacaggtt caatttcaat atccgccagc tccagttcac gtcccgtttc
    12961 acgagcgaga atcaatagtt tacgcgccac atccatacca gaaagatcat ctcgcgggtc
    13021 cggttcggta taacccattt cccgcgccag cgtggtcgcc tcggagaaac tcatgccttc
    13081 gtctaacttg ccgaagatat aagaaagcga acctgaaaga atgccggaga acttcaccaa
    13141 ttcatcacct gcattgagca gattttgcag gttctcaata accggtaatc cagccccaac
    13201 gttggtgtca tagaggaatt tacgccgcga tttttccgcc gcataacgca actgatgata
    13261 gtaatccatc gacgaggtgt tggccttttt gttcggcgtg acaacgtgga aaccttcgcg
    13321 caggaagtcg gcatattgat ccgccactgc ctggctggag gtgcagtcaa caatgaccgg
    13381 gttcagcaga tgatattctt tcacgaggcg aattaagcgc ccgagattaa acggctcttt
    13441 ggcttgtgca agttcttcct gccagttttc cagattaagg ccatgcacac tagtgagcag
    13501 agccttcgag ttggcaacgc cgcagacacg taagtcgata tgtttattct tcagccagct
    13561 ttgctgacgc ttcagttgct ccagcagcgc accgccaacg ccaccgacgc caatcacaaa
    13621 cacttcgata acctgatcgg tattgaacag catctgatga gtaacgcgca cgccagtggt
    13681 cgcatcatcg ttatttacca cgacagagat tgagcgttca gaagatccct gagcaatggc
    13741 gacaatgttg atattggcgc gggccagcgc ggcaaagaat ttcgccgaga tcccacgcaa
    13801 ggtgcgcata ccatcaccta ccaccgagat aatggccagc cgttccgtca ccgccagcgg
    13861 ctccagtaag ccttctttca gttccaggta gaactcttcc tgcattgccc gttcagctcg
    13921 cacacagtcg ctttgtggaa cgcagaaact gatactgtat tcggaagatg attgcgtaat
    13981 cagcaccacg gaaatacggg cgcgtgacat tgcagcaaag acgcgcgccg ccatgccaac
    14041 catccctttc atccccgggc cggaaacgct gaacattgcc atgttattca gattggaaat
    14101 gcccttgacc ggtaattcgt cttcatcacg gctggcacca atgagcgtac ctggtgcttg
    14161 aggatttccg gtatttttaa tcaggcaagg gatctggaac tgggcgatgg gggtaatggt
    14221 gcgggggtga agaactttag cgccgaagta ggaaagctcc atcgcttcct ggtaggacat
    14281 cgacttcaac aacctcgcat cgggcacctg acgcgggtcg caggtataga ccccgtcaac
    14341 gtccgtccaa atctcgcaac aatcggcgcg taaacaggca gccagcaccg ccgcggagta
    14401 gtcggaaccg ttacgtccaa gcaccaccag ttcgcctttt tcattaccgg cggtgaaacc
    14461 tgccatcaac accatgtgat cagccggaat gcggcttgcc gcaatacggc gggtggactc
    14521 agcaatatcg acggtagatt cgaggtaatg ccccactgcc agcagttttt cgaccggatc
    14581 gataacggta acgttgtgac cacgcgcttc taacacgccg gccataatgg cgatcgacat
    14641 tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc gggcactgcc ccaacaaact
    14701 aatgccatgc aggacatgtt ttatttgggc aaattcctga tcgacgaaag ttttcaattg
    14761 cgccagcggg aaccccggct gggcggcggc gagtcccgtc aaaagttcgg caaaaatacg
    14821 ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta atggtttttt caatcatcgc
    14881 caccaggtgg ttggtgattt tggcgggggc agagaggacg gtggccacct gcccctgcct
    14941 ggcattgctt tccagaatat cggcaacacg cagaaaacgt tctgcatttg ccactgatgt
    15001 accgccgaac ttcaacactc gcatggttgt tacctcgtta cctttggtcg aaaaaaaagc
    15061 ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta cgcgtcagcc cgcaccgtta
    15121 cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa tgcgtttcat ggatgttgtg
    15181 tactctgtaa tttttatctg tctgtgcgct atgcctatat tggttaaagt atttagtgac
    15241 ctaagtcaat aaaattttaa tttactcacg gcaggtaacc agttcagaag ctgctatcag
    15301 acactctttt tttaatccac acagagacat attgcccgtt gcagtcagaa tgaaaagctg
    15361 aaaaatactt actaaggcgt tttttatttg gtgatatttt tttcaatatc atgcagcaaa
    15421 cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca ggcgttgctg taaccagtcg
    15481 accagtttta tgtcatctgc cactgccaga gtcgtcagca atgccatgac tcgttcgcgt
    15541 aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt tcgccggttg ttgtattaat
    15601 gttgctaatt gatagcaata gaccatcacc gcctgcccca gattgagcga aggataatcc
    15661 gccaccatcg gcacaccagt aagcacgtca gccaacgcta actcttcgtt agtcagcccg
    15721 gaatcttcgc gaccaaacac cagcgcggca tggctcatcc atgaagattt ttcctctaac
    15781 agcggcacca gttctactgg cgtggcgtag taatgatatt tcgcccgact acgcgcagtg
    15841 gtggcgacag tgaaatcgac atcgtgtaac gattcagcca gtgtcgggaa aactttaata
    15901 ttatcaataa tatcaccaga tccatgtgcg acccagcggg tggctggctc caggtgtgcc
    15961 tgactatcga caatccgcag ttcgctaaaa cccatcgttt tcattgcccg cgccgctgcc
    16021 ccaatatttt ctgctctggc gggtgcgacc aggataatcg ttatacgcat attgccactc
    16081 ttcttgacca aataaccgcg aacagggtga tcactgtcaa cttattacgc ggtgcgaatt
    16141 tacaaattct taacgtaagc cgcagaaaaa gccctttact tagcttaaaa aaggctaaac
    16201 tattttctga ctgcactaac ggtttaattg ttaaaaaatg ctacatatcc ttctgtttac
    16261 ttaggataat tttataaaaa ataaatctcg acaattggat tcaccacgtt tattagttgt
    16321 atgatgcaac tagttggatt attaaaataa tgtgacgaaa gctagcattt agatacgatg
    16381 atttcatcaa actgttaacg tgctacaatt gaacttgata tatgtcaacg aagcgtagtt
    16441 ttattgggtg tccggcccct cttagcctgt tatgttgctg ttaaaatggt taggatgaca
    16501 gccgtttttg acactgtcgg gtcctgaggg aaagtaccca cgaccaagct aatgatgttg
    16561 ttgacgttga tggaaagtgc atcaagaacg caattacgta ctttagtcat gttacgccga
    16621 tcatgttaat ttgcagcatg catcaggcag gtcagggact tttgtacttc ctgtttcgat
    16681 ttagttggca atttaggtag caaacatgca gaccccgcac attcttatcg ttgaagacga
    16741 gttggtaaca cgcaacacgt tgaaaagtat tttcgaagcg gaaggctatg atgttttcga
    16801 agcgacagat ggcgcggaaa tgcatcagat cctctctgaa tatgacatca acctggtgat
    16861 catggatatc aatctgccgg gtaagaacgg tcttctgtta gcgcgtgaac tgcgcgagca
    16921 ggcgaatgtt gcgttgatgt tcctgactgg ccgtgacaac gaagtcgata aaattctcgg
    16981 cctcgaaatc ggtgcagatg actacatcac caaaccgttc aacccgcgtg aactgacgat
    17041 tcgtgcacgc aacctgctgt cccgtaccat gaatctgggt actgtcagcg aagaacgtcg
    17101 tagcgttgaa agctacaagt tcaatggttg ggaactggac atcaacagcc gttcgttgat
    17161 cggccctgat ggcgagcagt acaagctgcc gcgcagcgag ttccgcgcca tgcttcactt
    17221 ctgtgaaaac ccaggcaaaa ttcagtctcg tgctgaactg ctgaagaaaa tgaccggccg
    17281 tgagctgaaa ccgcacgacc gtactgtaga cgtgacgatc cgccgtattc gtaaacattt
    17341 cgaatctacg ccggatacgc cggaaatcat cgccaccatt cacggtgaag gttatcgctt
    17401 ctgcggtgat ctggaagatt aatcggcttt accaccgtca aaaaaacggc gctttttagc
    17461 gccgttttta tttttcaacc ttatttccag atacgtaatt catcgtccgt tgtaacttct
    17521 ttactggctt tcattttcgg cagtgaaaac gcataccagt ctatattacg agtcacaaac
    17581 atcatgccgg ccagcgccac caccagcaca ctggttccca acaacagcgc gctatcggca
    17641 gagttgagca gtccccacat cactccatcc agcaacaaca gcgcgagggt aaacaacatg
    17701 ctattgcgcc agcctttcaa taccgcctgc aaataaatac cgttcattaa cgccccaatc
    17761 agactggcga ttatccatgc cacggtaaaa ccgatatgtt cagaaagcgc cagcaagagc
    17821 aaataaaaca tcaccaatga aagccccacc agcaaatatt gcattgggtg taaacgttgc
    17881 gcggtgagcg tttcaaaaac aaagaacgcc ataaaagtca gtgcaatcag cagaatggcg
    17941 tacttagtcg cccggtccgt taattggtat tgatcggctg gcgtcgtcac tgcgacgcta
    18001 aacgcgggga agttttccca gccggtatca ttgcctgaag caaaacgctc accgagatta
    18061 ttagcaaacc agctgctttg ccattgcgcc tgaaaacctg actcgctagc ttcccgtttg
    18121 gctggtagaa aatcacctaa aaaactggga tgcggccagt tgctggttaa ggtcatttcg
    18181 ctattacgcc cggcaggcac cacagaaaga tcgccggtac cgcttaaatt cagtgccata
    18241 ttcagctgca ggttctgctt ccgccagtcc ccttcgggta aagggatatg cacgccctgc
    18301 ccgccttgct ctaacccggt accgggttca atggtcagtg ccgttccgtt aacttcaggc
    18361 gctttcacca caccaatacc acgcgcatcc ccgacgctaa tcacaataaa gggcttgcct
    18421 aagatgatat ttggcgcgtt gagctcgcta agacgcgaaa catcgaaatc ggcttttagc
    18481 gttaaatcac tgtgccagac ctgaccggta taaatcccta tcttgcgttc ttccacgttc
    18541 tgattgccat caaccatcaa tgactcaggt aaccaaaaat ggataaaact tcgtttccgc
    18601 tccacggttt tatcctcttc ctgcaccgta taaagctcgg tcacaggaat agcgataagc
    18661 ggcccaacga gtttttgcgg cccgctggta ctttggcgaa tcgcatcttc cacatcgcta
    18721 cggtaatcgg cacgttcgac aatcacttgc cgaatcagca ttatcggaat caacaacagc
    18781 aatactgcac caaacaaggt ggtcattttc cagaacaggg gggatttcaa catagtcttc
    18841 tcctttgcaa tggcagcagg atacgaaggc tatgtgggaa gaatttgaag ctatgtgaag
    18901 tgacggtgaa gtcgtagcga ggccagcacg ccaccttcct gcacgttgcg cagcgtgact
    18961 tcgccgttaa acaaacgggc gacctcactg acgaacgcca accccagacc gctgcttttt
    19021 tgcccatttg cacgcggcaa agagtaaaag cgttcaaaaa tacgtgacag cgcgtagtca
    19081 ggaataccac taccggtatc cagcacctta agcgtgacgt gttcctgatc cacttcggcg
    19141 cttagcgtta tgcaaccgct ctcgggagta aaatcgatgg cgttatccag taaattcccc
    19201 agcgcctgat ccagtaacgc cggttcagca gcaacattaa cctcggtggg cataacatgc
    19261 aaagtgatgt ttttttctgc caactgcacg gtgcgcgctt cgctgacgcg gcgaaataat
    19321 gccgccacat caacagcagt cagaacgact tcctgacgat tctccagtct tgcctggcgt
    19381 agtaacgttt ccaccagcgc ctgcattcgc gcattttgtg tcagaatgtt gtcagtaaaa
    19441 cgagccacta cttcaggcgg cggaccttcg cgtaaaattt ccgccgcgcc acgaatcgcc
    19501 gccagtgggc tttttagctc atgggttaac gcataaacat actgctcaat atagtttttc
    19561 ccttccagct tcacgcgcat actttccagc gcctgcgcga gtttacgcag ctcgctacta
    19621 ccgagatcgg gaagaggaac gggcttattg tcagtgacgg aatcagcata gcgagtgagc
    19681 cgggcaatgg agcggttgat ccaccaaacc atgcctgcgc caatcaccag tgcaatcccc
    19741 aacaaaatgg cgctggccca taaaattcgc cgctcgctgc gtttaatgac cggagccatc
    19801 gccgcgttcg gtttgcctac gctcaaaacg ccaataagcc gcgagccgcc cataatcggc
    19861 gcggcaacat acatcaccga actttcggga tcggcaggat tttgcaacgt gctgcgcgca
    19921 ccatactgac cacgcaacgt tagccagacg tcattccagc gcgaataatc ctgtccaacg
    19981 gctttatttg ccgaatcgaa caatactttg ccatgcgcat cggtcatata gacatgatat
    20041 tcattgcgca ctttgttaat gccaccaata ttggcgcgaa acgggcgatg ttgtagctga
    20101 ttaaacgcct gcgccagttg cccatgcgtt gggtccccag agagcaaatc gggacgcgcc
    20161 agctccgcaa gtaacgttgc cgtgtcgatc aacgtcccct ccgttgctct tcgcacgccc
    20221 ggtttaactt ctttgacaaa aatagccagt acgaaccagg ccgccaccgc caccagtaaa
    20281 aaatagccca gcagcaaccg catgccgata cgcattacaa gcccctcagg ctatatccca
    20341 tgccgcgatg agtattaatc ggtgaaagat cggggttgat ggcgcgcagc ttggcacgca
    20401 gcgttttaat gtggatatcg acggtgcgat cgtaggtgtc ctgcgcatct tcccacacgc
    20461 tatccattag ttgctggcgg gaccagacgc ggcctggcga cttgagtaac gtcttcagca
    20521 ataaaaactc ataccgcgtc agcgttaatg gcgtgtcaaa ccagctgatc tgcgccgcgg
    20581 gttcattcaa ttcaaaatga ccaatacgga tgacgggaga cggcgtcgag aacttcttca
    20641 cccgacgcag taaggtgcgc accctggcgc acacttcgcg gggtgaaaac ggtttagcca
    20701 catagtcgtc agcgccaatt tccagcccaa gcaggcgatc gacctcttca cttcgggccg
    20761 tcaggaacag tacaggtaac gccggatgga gcgccagtaa ctggcggcac aattcaaagc
    20821 cactgatatc aggcaaacca acgtcgagaa tcatgacgtc gggaacctgc tggcgagctt
    20881 tatccagcac cggcaaacct cgctcaaaga cctcgacggc aaaaccttcc tgctgcaaca
    20941 tgtagaccag cgtgtcggct atcccttgct catcttccac taaccagacc gtttcccgtt
    21001 gcataaatcg cctctgttat tgccgccacg gcatgacagg aaccgcgcta atcgcgtttt
    21061 tcggcgaacc ttctacaact ttgtcggagt aagccagata agcgagcgcg ttgcgtttgg
    21121 cgtcataaaa gcgcacgacc tgtaacgact taaagaccag ggacgtgcgt tttttgaata
    21181 ctacctcgcc ctgagctttg ccgtttttaa tacgatccga cagttcaatc ggcccgactt
    21241 gctgacaaga aatggccgca tcggaggtat cttctgccag gcccaatccc cctttaatac
    21301 caccggtttt cgcccggctc acataacagg tgacattttt cacatcggga tcgtcaaagg
    21361 cttccacaac aattttgtga tccgggccga tcattttaaa tacggtgtcg accgaaccaa
    21421 tctcttctgc atgagccaat ggccccagca ttatcaggct taaagacagg atcaaatgct
    21481 tgtatttcat attgttacca tttttatcta ttacacgagc aattagttcg tcacggtaaa
    21541 taacatattg attaagaaca aaatctcaat tcttttattt ccgtcaagcc ctaaaacata
    21601 ctctactaag aaaaaaacac tgaatgctaa aacagcaaaa aatgctatta tccaattacc
    21661 tgatgtcagg tgctcgttgt tgaaaggatg aggatatttt atggatcagg ccggcattat
    21721 tcgcgacctt ttaatctggc tggaaggtca tctggatcag cccctgtcgc tcgacaatgt
    21781 agcggcgaaa gcaggttatt ccaagtggca cttacagaga atgtttaaag atgtcactgg
    21841 ccatgctatt ggcgcgtata ttcgtgctcg tcgtttgtcg aaatcggcgg tcgcactacg
    21901 cctgactgcg cgtccgattc tggacatcgc gctgcaatac cgcttcgact ctcaacagac
    21961 atttacccgc gcattcaaga agcagtttgc ccagactcct gcactttacc gccgttctcc
    22021 tgaatggagc gcctttggta ttcgcccgcc gctacgtctg ggtgaattca caatgccaga
    22081 gcacaaattt gtcaccctgg aagatacgcc gctgattggt gttacccaga gctactcctg
    22141 ttcgctggag caaatctctg atttccgcca tgaaatgcgt tatcagttct ggcacgattt
    22201 tctcggcaac gcgccgacca ttccgccggt gctctacggc ctgaatgaaa cgcgtccgag
    22261 tcaggataaa gacgacgaac aagaggtatt ctataccacc gcgttagccc aggatcaggc
    22321 agatggctat gtactgacgg ggcatccggt gatgctgcag ggcggcgaat atgtgatgtt
    22381 tacctatgaa ggtctgggaa ccggcgtgca ggagtttatc ctgacggtat acggaacgtg
    22441 catgccaatg ctcaatctga cgcgccgtaa aggtcaggat attgagcgat actacccggc
    22501 agaagacgcc aaagcgggag atcgcccaat taatctgcgc tgtgaactgc tgattcctat
    22561 ccgccgttaa cgctgcagct catctaatgc aggggcgtcc agatgcgaga tgtcccctgc
    22621 ggtttcaacg acccagccag acgccagcca caggctttcc tgataatcca cgcgcgaaat
    22681 agaacagtta cgcagacgta agcggcgctc tgcccatgct ggtaatccga gaatcgtact
    22741 caccagacat cccagtgcaa taccatggct caccagcagc ggtcggcttc cctgcggtaa
    22801 gtctcggcaa gattccagtg ccgcattgac gcgatcgctg agctcctgca ttgactcgcc
    22861 ttcaggaata cgcccgtcaa cggtgccatt gaccagctgc cgacgccagt tctcttcttc
    22921 ttcggtcaga gaatcgatat gtcttttttc cagcacaccc atgtttaatt cacgcaggcg
    22981 agaatcaaag atgatgtcac agccgcaggc ctgggcgatg atttccgccg tacgccgggt
    23041 gcgtcctaaa tcgctactga taatatgcgt aatgccaagc tctttggcac gggttgccac
    23101 ctgcatcgcc tgttgctcac ctttggcggt cagcgggctg tcagactggc cctgaatacg
    23161 tcgctcggcg ttccactgcg tttcaccgtg gcggactagg tatacctgta acatgctttt
    23221 tttccgtata ctgctgtaac gttaattttc tgagtaatgc tgattatgca ccaagttgtc
    23281 tgtgcgacca ccaatcccgc taaaattcag gccattctgc aggcatttca cgagatcttc
    23341 ggcgaaggat cctgccatat tgcatccgtt gccgtcgaga gcggtgtacc ggaacaaccc
    23401 tttggcagtg aggaaacgcg cgctggcgca cgaaatcggg tagcaaatgc ccgccgttta
    23461 cttccagagg ctgatttttg ggtggcgatt gaagctggca ttgatggcga cagcactttc
    23521 agttgggtgg tgattgaaaa cgccagccag cgcggcgagg cgcgttcggc aactttgccg
    23581 ttacctgcgg tgattctgga aaaagttcgt gaaggcgagg cgcttggtcc cgtgagtcgc
    23641 gttataccgg tattgatgag attggtcgta aggaaggggc gatcggcgtg tttaccgccg
    23701 ggaaactcac tcgcgccagc gtctatcacc aggcggtgat cctggcgctg agtccgtttc
    23761 acaatgccgt gtatcaggca ttgtaggacg gataaggcgt tcacgccgca tccgacattc
    23821 ggtgcacgat gcctgatgcg acgctgccgc gtcttatcag gcctacaaaa tcaatcgctt
    23881 ttcagcaaca cctcttccag ccactgacgc agctccacgg gcgcggcttt caggctgtta
    23941 gatccacgcg taatcgtcgc gatgcccgcg ccgagttcat tttttaactc acgctgactc
    24001 atttcgccac gcaacagctc ttcgacaata cgcacgcgag tccccaacgc ttcgcgctca
    24061 tctggcgtca gcatcaggtt taacaacggt aaatggagat cgttttggta ggcattctta
    24121 agcaggtcga caaaacgtaa ccactcctgg tgacgctgtt ctgccatcgc tgctgaatag
    24181 ggtgattgtt gggccataat atgtcgccat tgttagcggg ggaagcaaaa tgactccccc
    24241 ggttgtactc gctaaagagt acgatagcat atcataaacg tgcggatcag taacgacgtc
    24301 cccattccgt ggcgctcatc aacgtcggtt tatcccccat gaaatagcgg tagtaagcat
    24361 cataagccag cacgttcttc acataaccgc gcgtctcgga gaacggaata ctctcgacaa
    24421 atgccacagc gtcgatacgc ccggcgctgt tgccaagcca ggttcgcacc cgtccaggtc
    24481 cggcgttata agctgctgag gagaaaatac ggttattgcc aaactgctga taaacatatt
    24541 gcaggtaact ggtgccaatg ttgatgttcg tttccggatc cagcaattgc ccagggctgc
    24601 tataaccagg aatagagaac atcttcaccg tatgggtcgc tgtaccaggc ataatctgca
    24661 tcaggccgct ggcccctacc ggtgatttca ctttcggatt ccaggcgctc tcctgacggg
    24721 caatcgccat cgcatagctt tgcgggatct ctttcccact ggtgtaacgt ttgaaaagat
    24781 cgttgtaagc cagcgggaat cgctcttcca gatgatccca aagcttcccg gcgatcgttg
    24841 cctgaacgct aagatcccac cattggttat tgaaagcata ccgagccagt tgagcctgct
    24901 ctgtttttga cttgctcttc accagattgg cccactcgct acgcgcggta ttatcgagat
    24961 tccagtacat caactcgcgc acgcgcgcca tctccggccc ctgagtcagg gtgctgtcaa
    25021 cattctgcgg cgctttatca atcttcagct catactcttc accgatgcgt tgtgctgcaa
    25081 ccatcgggta gaaaccacgc tgttgtatga gttgatgcaa aatctctttc gcttcagctt
    25141 cacgtccgcg ttccagcaat aaatccgcct gccagtaacg ccattcatct ttctcttttg
    25201 cttccatcgg cagacgcgcc agccaggtat tcaggccgcg acgatcgccg gtgccaagcg
    25261 ccattcgcac acggcgttca atcaacgacg tggattgcga gcgcataatg gcgtcatcgc
    25321 gccatttcgc ctgctcatcg gtgacatcat tgcccatcaa acgccaggcg acgatatcgc
    25381 gcagctcctg aatctgatct tcattaagct gctgcgcctg ggcaagcgac gggatcatca
    25441 gccgtgcatt ttcggcatcc tgccgcgcca cactggcaaa cgccaccgcc gccatttgac
    25501 gggtaaaatc ggtcgcgccg gttgtacgcg cgaaggtcag taccgtatta gggttgttcg
    25561 ccagtgaaat gattgccgag gcgatagtct ggtaatcggc aggcatctgc cctgccagca
    25621 ctgttaccag gcctgtgtta cccgctttca tcgccagacg gatacgctct aaatacgcca
    25681 gcggatcttg tttacctgac gcacgccaga cgctaaataa cttgtcacag gcgttaggct
    25741 ggctcttgcc ggttagccac agctctttcg ccccttgcca ggcttcttca ctctgcccgg
    25801 tgttccattt cgcatagtag taattacatt gcgcttcggt agttccgggc ttttccgggc
    25861 taaaggctaa caagccacgc cagtcttcac gccgcgccag ttcattgacg aaacgagatt
    25921 gcagcgtgcg agcgggagga agcgtggggt tagcgcgaac aaagttagtg accgtcaccg
    25981 ccggttgatt catcaggtca tcggtgatct ggcggtattc caggtaggga taaagcggat
    26041 aatccttcag tccaggcatc atttgttcga ccacatccat ctgtcgatta tcccaggcct
    26101 gcttgatttg cgcgtaacgg ctacgctgct catccagtga gtcggctcgc gccacgctgc
    26161 tgaccgtcag cagacagaca ccggcagcca acagccgcca ggtaacttgt ttggcttttt
    26221 ccacaagcgc atcctctaag tgtaaataca tcaatgcagc gtcatgccgc gtaatagatt
    26281 tatgctaacc agtcattgcc gtttacgcca cgttacggac acttttttac ttttactgcg
    26341 aaggtgatcg accaactggg attaggcggg gaaacaggct acacttcgcc tttgaaaact
    26401 catcatcacg ataaacatag aggcgaagtc caacgtggct caattcgttt ataccatgca
    26461 tcgtgtcggc aaagttgttc cgccgaaacg tcatattttg aaaaacatct ctctgagttt
    26521 cttccctggg gcaaaaattg gtgtcctggg tctgaacggc gcgggtaagt ccaccctgct
    26581 gcgcattatg gcgggcattg ataaagacat cgaaggtgaa gcgcgtccgc agccagacat
    26641 caagattggt tatctgccgc aggaaccgca gctgaacccg gaacacaccg tgcgtgagtc
    26701 cattgaagaa gcggtttcag aagtggttaa cgccctgaaa cgcctggatg aagtgtatgc
    26761 gctgtacgcc gatccggatg ccgattttga caagctggcc gctgaacaag gccgtctgga
    26821 agagatcatt caggctcacg acggtcataa cctgaacgta cagctggagc gtgcggcgga
    26881 tgcgctgcgt ctgccagatt gggacgcgaa aatcgctaac ctctccggtg gtgagcgtcg
    26941 tcgcgtggcg ttgtgccgcc tgctgctgga aaaaccagac atgctgctgc tcgacgaacc
    27001 gaccaaccac ctggatgccg aatccgtggc gtggctggaa cgcttcctgc acgacttcga
    27061 aggcaccgtg gtggcgatta ctcacgaccg ttacttcctc gataacgttg caggctggat
    27121 cctcgaactg gaccgcggtg aaggaattcc gtgggaaggt aactactcct cctggctgga
    27181 gcagaaagat cagcgtctgg cgcaggaagc ttcacaagaa gcggcgcgtc gtaagtcgat
    27241 cgagaaagag ctggagtggg tacgtcaggg aactaaaggc cgccagtcga aaggtaaagc
    27301 acgtctggca cgctttgaag agctgaacag caccgaatac cagaaacgta acgaaaccaa
    27361 cgaactgttt attccacctg gaccgcgtct gggcgataaa gtgctggaag tcagcaacct
    27421 gcgtaaatcc tacggcgatc gcctgctgat tgatgacctg agcttctcga tcccgaaagg
    27481 ggcgatcgtc gggatcatcg gtccgaacgg cgcgggtaaa tcgaccctgt tccgtatgat
    27541 ctccggtcag gaacagccgg acagcggcac catcactttg ggtgaaacgg tgaaactggc
    27601 atcggttgat cagttccgtg actcaatgga taacagcaaa accgtttggg aagaagtttc
    27661 cggcgggctg gatattatga agatcggcaa caccgagatg ccaagccgcg cctacgttgg
    27721 ccgctttaac tttaaagggg ttgatcaggg taaacgtgtt ggcgaacttt ccggtggtga
    27781 gcgcggtcgt ctgcatctgg cgaagctgct gcaggttggc ggcaacatgc tgctgctcga
    27841 cgaaccaacc aacgacctgg atatcgaaac cctgcgcgcg ctggaaaacg ccctgctgga
    27901 gttcccgggc tgtgcgatgg ttatctcgca cgaccgttgg ttcctcgacc gtatcgccac
    27961 gcacatcctg gactaccagg atgaaggtaa agttgagttc ttcgaaggta actttactga
    28021 gtacgaagag tacaaaaaac gcacgctggg cgcagacgcg ctggagccga agcgtatcaa
    28081 gtacaagcgt attgcgaagt aatgcgtaaa atgccggatg cggcgcgaac gccttatccg
    28141 gcctacaaaa ccagcaattt caatacgttg taaaactgta ggcctgataa gacgcggtaa
    28201 gcgtcgcatc aggcattgag caccgaatgc cggatgcggc gtgaacacct tttccggtct
    28261 acaacagcct tagcatcctc aatttcgtgg cgggtcaccc gtgcaggttt tttcaaacct
    28321 tgtttaatct cgacagcctc ttccagagag gttttcaact catcaaaaaa actcatttta
    28381 tacctcgcct ttgagcagtt tcgtcacggt tatccttgct cccccatcat ctcccgcacc
    28441 agctccacgc agcgcaggaa acggctgtcg taatcgtcct cttcaacccg cacgaattcg
    28501 atattgttct cttccagcat ctccaccagc aagttctgga actctttgcg atccaccgaa
    28561 ctgccgaggc tgcgtaaacc atccgccacc cacggcgtgt tgttctccag caggatcacc
    28621 agatcgaaac ggtattcatc aatcagcgcc tgcacgaacg gatgctcacg cccttcgtac
    28681 tttttgcaga acgcctgggt agtgacaaaa tcggtgtcaa taaacgccac tttattggcg
    28741 tatttcactg caaaatcaat gtattgtgcg tgacccaacg cgattttatc gtagtcggaa
    28801 tactgcaatg cgatctcatc accgccgagg tgtgaaaaga cataatcgcg gccatattcc
    28861 cacgcactgg tggtgttgaa gatattggca agtttgttta ccagggtgga tttaccgctc
    28921 gactcgccgc caaggatcgc cacggtacgc acaaaaaacg gcttcacttc ggtaggaata
    28981 tattcccagt agcggaacgg gttttcgcgg atctgcgcac cgctgatact cataaaggta
    29041 cgtttcggat cgaccagcac cgtctcgatc cccagatgtt ccatatactg tggcgcatcg
    29101 gcttcttccg aggtgtagat caggtccggc tggatcccct tttcagccat aaactttttg
    29161 atgccgttgc tccacacatc ccagccatgc ggatacggct ccatgccctc ttcgttgaaa
    29221 gcatgaatac ggatattttt ctgatattta aaggtttgca atagccagcg cagacgatcc
    29281 ggcaccgtgg gctgctgcga catggcgctg tcttcaaaca acgcgcggtc gcgggtatcg
    29341 tcaaaaccca taatgatatg cagctcgtca acctggctac aggcgcgctg gataaggtag
    29401 atatgtccgg tatgcagcgg gtagaactta ccgaatacga caccaatggt tttcttctgc
    29461 cggggaaatt caagccccaa aaaacggtgc aatgcctcca gcttttgcgc gctggggctt
    29521 ttaattttgg cattcagtaa ctggcttaaa taccctttgg tcataccgct ggcatcagct
    29581 acctgctgta gcgtgcagcc ctgttgcttg atggcagttt tcaggtaatc aaatgacgac
    29641 atatctccct ccgtatctct cattataagt cgtcgaacac gctaagcgcg tcggagagtt
    29701 ttttaacgcc aaaaatctgc atcccttccg gcgctttttt cggtacgtta gccgccggaa
    29761 caatcgcccg gcgaaaaccg tgtttcgccg cttcagaaat ccgctcctgg ccactgggca
    29821 ccgggcggat ctcccctgcc agcccgactt caccaaacac caccagatcc tgtggcagcg
    29881 gtctgtcacg caggctggaa accatcgcca gcagtaacgc taagtcggcg ctggtttcgg
    29941 ttaccttcac gccgccgacc acgttcacaa acacatcctg atcggccatt tgcagaccac
    30001 cgtgacggtg caagacagcc agcaggattg ccagacggtt ttgttccagc cccactgcca
    30061 cgcggcgcgg attcgccatt atcgagtgat cgaccagcgc ctgaatctcc accagcaacg
    30121 gacgcgttcc ttcccacacc accatcacgg agctgccgga ggtcacttca tctccgcgac
    30181 ttaagaaaat tgccgaaggg ttgctgactt cacgcagccc ctgttcggtc atcgcgaaga
    30241 cgcccagctc attcaccgcg ccgaagcggt ttttatggct gcgtaaggtg cgaaaacggg
    30301 agtcggcatc gccatccaga agcaccgaac agtcgataca gtgttccagc actttcgggc
    30361 cagccagcga accatctttg gttacgtgtc caaccatgac aatcgccaca ccgcgcgttt
    30421 tggcgaaacg cgtcagataa gccgccgttt cacgcacctg cgccacgcta ccaggcgatg
    30481 attgtacatc cgccatatgc atcacctgga tcgagtcaat taccatcagc ttcggttgct
    30541 cttcttcggc aatcaggcag atctgctcga tgctggtttc cgacaacata ttgagattgt
    30601 cagtcggcag gccaaggcga tgagcgcgca ttgccacctg ttgcagcgac tcttcgccgg
    30661 tgacatacag cgttttcatc tgctgggcca gtttgcacag cgtttgcagc aacagcgtgg
    30721 atttccccgc accagggtta ccgccaatca gaatggcact tcctggcacc acgccgcctc
    30781 ctagtacgcg gtcgaactct ttaaatccgg tggaaaaacg cggcagctct tcaaggctga
    30841 tatcggagag tttctggact tttgccaccc cggcgctacc ggcatagccg ctgagacgct
    30901 cgttacgcgc caccgttggc gacgcagcaa gacgcacctc ggtgatggtg ttccaggcat
    30961 gacaggcact gcactgcccc tgccagcgcg gataatcggc cccgcattca ttacaaacaa
    31021 aggcgcgttt tggagctttt gccacggtgt acctcgttaa tgctgtgccg cccgcaggat
    31081 gacgggcagg caattacttc tgattcaggc tgccagagag gatgcagaat acccccatca
    31141 ggtcagcgtg acggatggtg acttccgcct tttcattcac ttttggcttg gcatggtagg
    31201 caatccccag ccctgctgct ttgatcatcg gcaggtcatt agctccatcg ccaatcgcca
    31261 cggtctgcgc cagcgggatt tcatactcct gcgcgaggcg agtcagagtt ttcgctttgt
    31321 actgcgcgtc tacgatgtcg ccgatcacat tgccggtaaa tttaccgtcc atgatctcca
    31381 gttcattggc taccacggct gtcaggcgca gcttgtcgcg caggtattca gcaaagaaag
    31441 taaagccgcc ggaggcaatc gccactttcc agcccagcgt ttccagcttg agcaccagct
    31501 gcgttaagcc tggcatcagc ggcagatttt cacgcacctg ttgcagaata ttggcgtcag
    31561 cgcctttcag cgtcgccacg cggctgcgca ggctggcggt aaaatcgagt tcgccgcgca
    31621 tcgcccgttc ggttacctcc gccaccatct cgcccgttcc ggccagcttg gcaatttcat
    31681 caatacattc aatctggatg gcggtggaat ccatgtccat caccagcaaa cccggcgtgc
    31741 gcaggtgcgg gattttcccc agcggggcga catccagctg cgcttcgtgg gccaggcgtg
    31801 tagcccgtgc ggtgagtgaa cctgccagac gaatcacctg ataatcttcc acgcaccagg
    31861 cggcaacaat caccatcgcc gcacccagtt tgctctggta ttgggtcaga cgctgtttat
    31921 ccagcccacg accatacagc agccagccgc tacgacctgc gtggtaatcc agtggcatca
    31981 cttcatcacc acttaatgaa agaggcagac ccggccataa agagacatct tcaggcaggt
    32041 cgcaccaggt aatgttaggc attaaggctc ctgtaaaatc gttcgaagca gggaaaataa
    32101 cgcatgaggc taccttgtat ccattgcttc tggcaacatt aagtctcaaa ttttcaaagg
    32161 gtggaagatg gctcgcacaa aactgaaatt ccggctacat cgggcagtga ttgtcctgtt
    32221 ctgtcttgcc ttgttagtgg cactgatgca gggggcgtca tggtttagtc aaaaccacca
    32281 gcgacagcgt aatccacagc tggaagaact ggcccgcacc ctggcgcgtc aggtgacgct
    32341 gaacgttgca ccgctgatgc gtaccgactc accggatgaa aaacgcattc aggcgatcct
    32401 cgatcagtta acagatgaaa gccgtatcct cgacgcgggt gtgtatgacg aacaaggcga
    32461 tcttatcgca cgttctggcg aaagcgtcga agtgcgcgat cggctggcgc tcgacggtaa
    32521 aaaagcaggc ggctatttta accagcagat tgtcgagcca attgcgggta aaaacggacc
    32581 gctcggctat ctgcgcctga cactcgacac ccatacgctc gccaccgaag cccaacaggt
    32641 ggataacacc actaacattt tacgcctgat gttgctgctc tcactggcaa tcggcgtagt
    32701 gctgacccgc acgctgctac agggtaaacg cacccgctgg cagcaatcgc ccttcctgtt
    32761 aaccgccagc aaaccggtgc cggaagagga agaaagcgag aaaaaagagt gacccattac
    32821 tacaagaaag gaaatcgtta tgtccacatt acgcctgctc atctctgact cttacgaccc
    32881 gtggtttaac ctggcggtgg aagagtgtat ttttcgccaa atgcccgcca cgcagcgcgt
    32941 tctgtttctc tggcgcaatg ccgacacggt agtaattggt cgcgcgcaga acccgtggaa
    33001 agagtgtaat acccggcgga tggaagaaga taacgtccgc ctggcgcggc gcagtggcgg
    33061 tggcggcgcg gtgttccacg atctcggcaa tacctgcttt acctttatgg ctggcaagcc
    33121 ggagtacgat aaaactatct ccacgtcgat tgtgctcaat gcgctgaacg cgctcggcgt
    33181 cagcgccgaa gcgtccggac gtaacgatct ggtggtgaaa accgtcgaag gcgaccgcaa
    33241 agtctcaggc tcggcctatc gcgaaaccaa agatcgcggc ttccaccacg gcaccttgct
    33301 actcaatgcc gacctcagcc gcctggcaaa ctatctcaat ccggataaaa agaaactggc
    33361 ggcgaaaggc attacttcag tgcgttcccg cgtgaccaac ctcaccgagc tgctgccggg
    33421 gctcaccatg agcaggtttg cgaggccata accgaggcct ttttcgccca ttatggcgag
    33481 cacgtggaag cggaaatcat ctccccggac aaaacgccag acttgccaaa cttcgccgaa
    33541 acctttgccc gtcagagtag ctgagagtgg aacttcggtc aggctccggc attctcgcat
    33601 ctgctggatg aacgctttag ctggggcggc gtggaactgc atttcgacgt tgaaaaaggc
    33661 catatcaccc gcgcccaggt atttaccgac agcctcaacc ccgcgccgct ggaagccctc
    33721 gccgggcgac tgcaaggctg cctgtaccgc gcggatatgc tgcaacaaga gtgcgaagcg
    33781 ctgttggttg acttcccgga ccaggaaaaa gagctacggg agttgtcgac gtggatagcg
    33841 ggggcggtaa ggtaactcgc tgattaaaat atccaaggaa ggaaatatga tcgatctgga
    33901 aaaccaggaa cgggagatta tcaatttaat gttcagccag cgtatttcct ggctagccgc
    33961 cgtgcgtatc aggcataaat tatcgcttgc tgaagtaagt aaaatgttag gtatctcgat
    34021 taattctcta aaacagatcg aaaagacgga acggcttagc tcaaacatca aaagcaaaat
    34081 ggcagaaatt tatggttgtc caccagagtt gctgatatgc ccttcctgga tgacggctga
    34141 acataaatga atagattcgt gaatagatac ttgaatgttt tttattttct atccatatga
    34201 aaaataatag taaatctata aatcagttgc tattgatgaa tagatactaa aaagccggag
    34261 cagtccccca ctccggctta acacgacaat cgcctttcag cgaaacacaa ttactcttta
    34321 tcgcccagca gaacggattc cagtgcgatt ttgatcatgt cgttgaaggt ggtctgacgc
    34381 tcagcggcag tggtctgctc gtgagtgcgg atgtggtcag acacggtgca gatggtcagg
    34441 gctttcgcgc caaactctgc agccacgccg tagataccag ccgcttccat ttccacgccg
    34501 aggatgccgt atttttccat cacgtcgaac atttcgccgt ccggagagta gaacaggtca
    34561 gcggagaaca ggttacccac gcgagcatca acgcccagtg ctttagctgc atctactgcg
    34621 ttacgcacca tgtcaaagtc agcgatagcg gcaaagtcat ggtctttaaa acggatgcgg
    34681 ttaactttgg aatcggtgca ggcccccata ccgataacga cgtcgcgcag ttttacgtgc
    34741 ggcagaactg cgccacagga accaacgcgg ataattttct tcacgccgaa atcggtgatc
    34801 agttctttgg tgtagatgga gcaggacggg atacccatac cgtgacccat tacggaaatt
    34861 ttgcggcctt tgtaagtacc agtgaagccc agcataccgc gaacgttgtt cacttcacgg
    34921 gcatcttcaa ggaaagtttc agcaatatac ttcgcacgca gcgggtcgcc tggcatcaaa
    34981 actacgtcag cgaaatcgcc catttctgca ttaatgtgtg gggtagccat tgttttatcc
    35041 tttttgtgac ataacaaagg cggagtcaac gctccgccca aatccatcag aacatggctt
    35101 tgccatattc catatcagaa gtaccaaaat attttgccag agtctggccg atatccgcga
    35161 aggtttcacg gtgacccagt gagcccggtt ttactttcgg gccatatacc agtaccggaa
    35221 tgtgttcacg cgtgtggtca gtaccggtcc aggtcggatc gcaaccgtgg tcagcggtga
    35281 ggatcaggat gtcgtcatcg cgcagcagag acatcagctc cggcagacgg cggtcgaaca
    35341 gttccagacc cgcagcataa ccggcgacgt cgcggcggtg gccccaggaa gagtcgaagt
    35401 caacgaagtt ggtgaagacg atggtgttat cacccgcttc tttcatctct ttgatggtgg
    35461 cgtcaaacag cgcgtccaag ccagtcgctt tcactttttt ggtgataccg cagttggcgt
    35521 agatgtctgc aattttaccg acagaaacca cctggccgtg tttttcatca accagtttct
    35581 gcagcacggt cggtgctggc ggctcaacag ccaagtcgtg acggttaccg gtacgctgga
    35641 agttaccagc tttgtcaccg ataaacggac gagcgataac acgaccgata ttgtagccgc
    35701 cgttggtcag ctcttcacgg gcgatttcgc acagttcgta gagtttatcc agaccgaaag
    35761 tttcttcatg gcaggcaatc tggaacacgg agtcagcgga ggtatagaaa atcggcttgc
    35821 cggttttcat gtgctcttcg cccagttgat ccagaatgac cgtaccggaa gagtggcagt
    35881 taccgaggta acccggcaga ttagcgcgtt cgaccagttt atccagcagc tcttgcggga
    35941 agctgttttc gtgatcggag aaatatcccc actcaaacag aaccgggaca ccggcgattt
    36001 cccagtgacc agacggggta tctttaccgg atgacatttc gtgcgcccat gcgtacgcgc
    36061 cgataacttc agcgttgccg tccattcccg ccggaatgaa accggtagaa ccttcgtgtg
    36121 ctttcgccag ccccagacgg gtcagatttg gcagattgag cggcccttta cgaccattat
    36181 cagcttcgcc tttggcacaa gcttctgcga tatgacccag ggtgtcagcc ccgacgtcac
    36241 caaagcgttc tgcatcttct gtagcgccga tgccgaatga gtccagcacc ataataaatg
    36301 cacgtttcat atgttctccg tactcagtgc ttcaaattta aatgatcaga tcagtatacc
    36361 gttattcgct gatacggcga tagacagttg gtgtgctttc cggtgcgtta tcggcaagtt
    36421 taattgccgc tttcaccgct ttcgccgctt cctgccagct gttttcgtct ttcgcgagga
    36481 taaccgccag cggacgctga ccgtctacct ggtcgcccag acgcgccata tcagtaaagc
    36541 cgacgctgta atcgatggtg tcagatgcct gacgacgacc gccgcccatt gcaactactg
    36601 ccatccccag cgcgcgggta tccatttcac tgacaaaacc ttcggtatca gcatagactg
    36661 ctttcgtcag catcgctgtc ggcagatact tcgcgtagtt ctcaacgaag tcggtcgggc
    36721 ctttttgtgc cgctaccata cgaccaaaga cttctgccgc tttaccgttg tccagcaccg
    36781 cctgcaattt cgcgcgcgct tcggcgtcat ctttcgccag tttgccggag atcaacatct
    36841 ccacgcacag cgccatcgtg acatcaaaca gacgcgggtt acgatactca cccgtcagga
    36901 actgcaccgc ttcacgaact tcaaccgcgt tacctgcact ggaggccagt acctgattca
    36961 tgtcggtgag cagcgcggtg gtgcgcacgc cagcgccgtt agccacgcca acaatcgctt
    37021 cggcaagggc ttcagagagt tcgtaggtcg gcataaacgc gccgctaccc actttcacgt
    37081 ccatcaccag cgcgtccaga ccttccgcca gtttcttcgc caggatcgag gcagtgatca
    37141 gcgggatgga gtccacggtt gcggtaatat cacgggtcgc gtagaaacgt ttatcagccg
    37201 gagccagtga actggtctga ccgataatcg ccacgccgac gtctttaata atttcgcgga
    37261 aacggttgtc atccgggaaa atgtcgaagc cagggatgga ttccagtttg tcgagcgtac
    37321 cgccagtatg accgaggccg cgaccggaga tcatcggaat atagccgccg caggctgcga
    37381 ccatcggccc caacatcagc gaagtgacat cgccgacgcc gccggtcgag tgtttatcaa
    37441 caatcgggcc attcagatgc aggcttttcc agtcgagaac ggttcctgaa tctcgcatcg
    37501 ccatggtcag cgagacacgc tcaggcattg tcatatcgtg gaagaaaatg gtcatcgcga
    37561 gggcggcaat ctgcccttcg gaaatagtgt tgtcgcgaat accgttgata aagaaacgaa
    37621 tttcttcatc gctcagcgca tgaccatcac gttttttacg aataatttct tgtgcgagaa
    37681 acaaggtaac ctcccgagga aaagagtatg aggcggcttt tgccggatgc gtctacgcct
    37741 tatccggcct acgggtagat acgtctcgta ggcctgataa gcgcagcgca tcaggcgtaa
    37801 agcatcttac ttagtagctg ctggcgctct taccgtcgcc gtggcccagc gctttcagca
    37861 ggcttgccag caggctggaa gcaccaaagc ggtagtgacg cgcatcagcc cagtcagcac
    37921 cgaacagttc atctgcgatg gcgagatatt tctgcgcatc ttccgcagta cgcacgccgc
    37981 ccgccggttt gaaaccaacg gttttttcta cgcccatatc acggatcact tccatcatga
    38041 tgcgagcgct ttccggcgtc gcgttcacag ccactttacc ggtagaggtt ttgatgaagt
    38101 ccgcacccgc tttgatggag atttcagacg ctttacggat cagcgcttcg tctttcagtt
    38161 cgccggtttc gatgatcact ttcagcagta cattcgctgc cgcgcaagcc tctttacagg
    38221 ctttcaccag gtcaaaacca acctgctcgt cacccgccat cagcgcgcgg tacgggaaca
    38281 ccacgtcaac ttcatcagca ccgtaggcga ttgccgcacg ggtttctgcc agcgcgattt
    38341 cgatgtcgtc gttaccgtgt gggaagttgg ttaccgtagc gatacggatt tccggggtgc
    38401 cctgctcttt cagagttttg cgagcaatcg ggataaaacg aggatagata cagatagcgg
    38461 cggtattgcc gaccggagtt ttggcctgat gacacagagc aattactttc tcgtcggtgt
    38521 cgtcgtcatt cagggtggtc aggtccatca atttcagtgc acgcaggctg cttgctttca
    38581 gatcagtcat ttcattctcc tggcttgtcg ccaataaaat tcaccttgcg agtttgttag
    38641 tattctaaca tctactccgc aacacacttc gatacacatc acaattaagg aaatctactt
    38701 acaagtttgc atcactgtaa tgcgatctgg ttcaaataat tcactttcaa atgaatgcgt
    38761 cagtggtggc aaacgcatca ggatcaaagt gaacatcacg aaacttctta caatggcgca
    38821 tcaccttcgg cataaaagga aaagaagatg cccacttctc atgaaaatgc actgcaacaa
    38881 cgttgccagc aaattgtcac cagcccagtg cttagcccgg agcagaagcg ccattttctg
    38941 gcactggaag cagaaaacaa tctgccttac ccacagcttc ctgccgaagc ccgccgcgcg
    39001 ctggatgaag gtgtaatctg cgatatgttt gaaggtcatg cgccgtacaa accgcgctat
    39061 gtcttacccg attacgctcg ttttctggcg aacggttccg aatggctgga gctggaaggc
    39121 gcgaaagatc ttgatgacgc actctctctg ctgaccattc tttaccacca cgtaccgtcg
    39181 gtcacatcga tgccggtcta cctggggcaa ctggatgcgt tgttgcaacc gtatgttaga
    39241 attctaacac aaaacgagat cgatgttcga ataaaacgtt tctggcgtta cctcgacaga
    39301 accctgccag atgcctttat gcacgccaat atcggcccgt ctgattcgcc cattacccgc
    39361 gcgatcttac gtgcagacgc gcaggtttca ccgaacctga cctttatcta cgatcctgaa
    39421 atcacccctg atgacctgct gctggaagtg gcgaagaaca tctgtgaatg tagcaaaccg
    39481 cacatcgcca acggtccggt gcatgataaa attttcacaa aagggggcta cgggattgtg
    39541 agctgttaca actcactgcc gctggcgggt ggtggcagca cgctggtacg ccttaacctg
    39601 aaagccattg ccgagcgcag cgaatcgctg gatgacttct ttacgcgcac tctaccgcac
    39661 tactgccagc agcagatcgc catcatcgat gcgcggtgtg aattcctcta tcaacaatca
    39721 cacttctttg agaatagctt cctggtgaaa gaagggctga ttaaccctga acgttttgtg
    39781 ccaatgtttg gcatgtatgg gctggcggaa gcggttaact tgctgtgtga gaaagaaggg
    39841 attgccgcgc gctacggtaa agaagccgcc gcaaatgaag tgggttatcg catcagcgcg
    39901 caactggcgg agtttgtcgc caataccccc gtgaaatatg gctggcaaaa acgcgccatg
    39961 ttacacgcac agtcggggat cagttccgat atcggcacca cgccgggcgc gcgtttgccg
    40021 tatggcgatg agccagatcc gatcacccat ctgcaaactg tcgccccgca tcatgcttat
    40081 tattattccg gcatcagcga cattctgacg ctcgacgaaa ccatcaaacg taacccgcag
    40141 gcactggtac agctttgcct cggtgccttt aaagccggaa tgcgtgaatt taccgccaat
    40201 gtcagcggta acgatctggt tcgcgttacc ggttatatgg tgcgtttgtc ggatttagaa
    40261 aaatatcgcg ccgaaggttc acgcaccaac accacctggc tgggcgaaga agccgcacgc
    40321 aacactcgta ttctggaacg ccagccgcgc gtgataagcc atgaacagca gatgcgcttt
    40381 agtcagtaag attatcccct tctcctgcgt tgacgggcca ggcagtcgtc tggctctgtt
    40441 tttgcagggc tgcaatctgc gctgcaaaaa ctgtcacaat ccgtggacga tgggacgttg
    40501 caatgactgt ggggagtgcg tgccacagtg tccgcatcag gcgttgcaga ttgttgacgg
    40561 caaagtggtg tggaacgctg tggtctgcga gcaatgtgat acctgcctga agatgtgtcc
    40621 gcaacatgcc acaccaatgg cgcaatccat gagcgtggac gaagtgctta gccatgtgcg
    40681 caaagcggtg ctgtttattg aagggataac ggtaagcggc ggcgaagcca cgacccaact
    40741 gccgtttgta gtggcgctgt ttactgctat caaaaacgat ccgcaactgc gccatctcac
    40801 ctgtctggtg gacagtaacg gtatgttgag cgaaaccggc tgggaaaaat tactcccggt
    40861 gtgtgacggc gcaatgctcg atctcaaagc atggggtagc gaatgtcatc aacaattaac
    40921 cggacgcgat aatcaacaga ttaagcgcag catctatttg ctggcagagc gcggcaagct
    40981 ggcggaactg cgtttgctga tgatcccagg ccaggtggat tatttgcaac acatcgaaga
    41041 actggcggcg tttatcaagg gacttggtga tgttccggtt cgcctgaacg cgtttcatgc
    41101 ccacggcgtg tatggcgagg cgcaaagctg ggcgagcgcc acgccggaag acgttgagcc
    41161 gttggctgat gcattaaagg tgcgcggggt gagccggttg atatttccgg cgctctattt
    41221 gtgacatgcc ggatacggcc tacggttcgg cacagacttg taggcctgat aagacgcgtc
    41281 aagcgtcgca tcaggcattg tgcgccaact gccggatgcg gcgtgaacgc cttatccggc
    41341 ctacggttcg gcacaaacct gtaggcatga taagacgcgt taagcgtcgc atcaggcatt
    41401 atgcgccgac tgccggatgc tgcctacgaa gggcaaacta cccacgaacg ttaaacaccg
    41461 catacgtgtt attaagcaac acttcggcaa tctcatccgc gggttccggg cgtaactcgc
    41521 aaagcacggc aaacacacgg gcagcctgtt ccgggcggtt aggctgcccc tgaaaaccgt
    41581 tgagcggcat atccggcgca tcggtttcca gcaacaacga tgccagcggt aattttgcga
    41641 tgacatcgcg ggttttactg gcgcgtggat aggtgatagt accgcctacg ccgattttgt
    41701 agcccagctg tacaaaccgt tcagcctgtt gctggctgcc agaaaatccg tggacaacac
    41761 cagcacgcgg caaatcgtgg cgtttaagat gcatcgccag tttgtcgtgc gtgcgccgtg
    41821 aatgcaggat caccggcaga tcgtagcgtt tcgccagttt cagttgttcg tcgagtaacc
    41881 actgctgcct ctcaaattgc ggatcgtcgc cgaagagatc cagaccgatc tcccccaccg
    41941 ccaccacctt cgccggacgc ctttccagcg cctgctgtag ctgctcaaga gacacatcgc
    42001 tatgtttttc caacatacca ggatgcaagc ccaatacggc atacagcggt tgataatttt
    42061 ccgctaacgc ctgcacacgg gcaaaatttt ccgcctctgt cgccggaaca atgatcttgc
    42121 ctacgcccgc ttgtgccgcg cgttgcaggc tggcctcttc atcgccaata aaaggcggga
    42181 aatcaaaatg gcagtgagtg tcgataaaac ggcaaatcaa gccagatcct cattgttaaa
    42241 tgtcgggtcg ttcgcctgcg gagcgtcgct cacttcggca accagcgtat cgttggcgac
    42301 tggcgcaggc ggaatgacaa tcgattccgg cgtcaccact ggcaccagat ggcgggtaag
    42361 cggcgctttt tcagttaata gcttgccaac cgtggcgagg aaataacgac cgcataaacg
    42421 cccaagttta tagtcttcac gcagcgccgg aatccgactg ccaagcgcga tactatgtaa
    42481 tggcttcggc ggataaattt cggatatccg cagcttgccc ggtggtttct caataaattg
    42541 ctgaatgtca cgatagctgg tttcatgatg ctgcaccaga ttgaccagcg gctgcaggct
    42601 actgtcaccc agccagcgtt ccatgcgttt gaaccactgc ggcgtgtagt acatttgtga
    42661 cggcacagtg cggatgacga ccaacgtttt agcgccctgc cttgccgcct ctttaaccgg
    42721 aatcgcatca ctgatcccgc catccaggta gttaatgcct tccagcgaca ctccgctacg
    42781 ataaaagcca ggtatcgcac tggaggcgcg aatcacatcc agccagtttt gtttggttgg
    42841 taaaaagtaa ttcggcgcgt agtcatcctg acgacaggcg cacatataaa acgatttgcc
    42901 gctgtcaaac aaccgcgcgg cggtatccat tt
//
LOCUS       DADLTY010000009        42854 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.009, whole genome shotgun sequence.
ACCESSION   DADLTY010000009 DADLTY010000000
VERSION     DADLTY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42854
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.009"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..1100
                     /locus_tag="JN334_000428"
     CDS             <1..1100
                     /locus_tag="JN334_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436693.1"
                     /translation="DKALNMFDKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVL
                     SQRLHKAITESQSNYLSTLEGILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRS
                     ELNLINLLNSLNEVELMRKRLAFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELM
                     DKYFTTLLKEISNQPLEDWDIIISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHS
                     ESEMRGLFSSLAELYSFGNDTASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLI
                     KEEMLGMHNLILLENICMQKNAECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKI
                     YHKLEIKPEPEQQVLIQSLYDRASGSASATQ"
     gene            complement(1160..1651)
                     /gene="yajQ"
                     /locus_tag="JN334_000429"
     CDS             complement(1160..1651)
                     /gene="yajQ"
                     /locus_tag="JN334_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HAY7436694.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            1819..2730
                     /gene="panE"
                     /locus_tag="JN334_000430"
     CDS             1819..2730
                     /gene="panE"
                     /locus_tag="JN334_000430"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAY7436695.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            2693..3283
                     /gene="yajL"
                     /locus_tag="JN334_000431"
     CDS             2693..3283
                     /gene="yajL"
                     /locus_tag="JN334_000431"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HAY7436696.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3337..4785)
                     /gene="thiI"
                     /locus_tag="JN334_000432"
     CDS             complement(3337..4785)
                     /gene="thiI"
                     /locus_tag="JN334_000432"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAY7436697.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            4991..5233
                     /gene="xseB"
                     /locus_tag="JN334_000433"
     CDS             4991..5233
                     /gene="xseB"
                     /locus_tag="JN334_000433"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAY7436698.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5233..6132
                     /gene="ispA"
                     /locus_tag="JN334_000434"
     CDS             5233..6132
                     /gene="ispA"
                     /locus_tag="JN334_000434"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HAY7436699.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6157..8019
                     /gene="dxs"
                     /locus_tag="JN334_000435"
     CDS             6157..8019
                     /gene="dxs"
                     /locus_tag="JN334_000435"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAY7436700.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8074..8991
                     /gene="yajO"
                     /locus_tag="JN334_000436"
     CDS             8074..8991
                     /gene="yajO"
                     /locus_tag="JN334_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HAY7436701.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(9102..9620)
                     /gene="pgpA"
                     /locus_tag="JN334_000437"
     CDS             complement(9102..9620)
                     /gene="pgpA"
                     /locus_tag="JN334_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAY7436702.1"
                     /translation="MTILSRHKNVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9598..10575)
                     /gene="thiL"
                     /locus_tag="JN334_000438"
     CDS             complement(9598..10575)
                     /gene="thiL"
                     /locus_tag="JN334_000438"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAY7436703.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(10653..11072)
                     /gene="nusB"
                     /locus_tag="JN334_000439"
     CDS             complement(10653..11072)
                     /gene="nusB"
                     /locus_tag="JN334_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAY7436704.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11092..11562)
                     /gene="ribE"
                     /locus_tag="JN334_000440"
     CDS             complement(11092..11562)
                     /gene="ribE"
                     /locus_tag="JN334_000440"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAY7436705.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(11651..12754)
                     /gene="ribD"
                     /locus_tag="JN334_000441"
     CDS             complement(11651..12754)
                     /gene="ribD"
                     /locus_tag="JN334_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HAY7436706.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(12758..13207)
                     /gene="nrdR"
                     /locus_tag="JN334_000442"
     CDS             complement(12758..13207)
                     /gene="nrdR"
                     /locus_tag="JN334_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAY7436707.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13358..13897
                     /locus_tag="JN334_000443"
     CDS             13358..13897
                     /locus_tag="JN334_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY7436708.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14195..15079
                     /locus_tag="JN334_000444"
     CDS             14195..15079
                     /locus_tag="JN334_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HAY7436709.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15256..15603)
                     /locus_tag="JN334_000445"
     CDS             complement(15256..15603)
                     /locus_tag="JN334_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HAY7436710.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(15732..16703)
                     /gene="secF"
                     /locus_tag="JN334_000446"
     CDS             complement(15732..16703)
                     /gene="secF"
                     /locus_tag="JN334_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAY7436711.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(16714..18561)
                     /gene="secD"
                     /locus_tag="JN334_000447"
     CDS             complement(16714..18561)
                     /gene="secD"
                     /locus_tag="JN334_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAY7436712.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18589..18921)
                     /gene="yajC"
                     /locus_tag="JN334_000448"
     CDS             complement(18589..18921)
                     /gene="yajC"
                     /locus_tag="JN334_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAY7436713.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(18944..20071)
                     /gene="tgt"
                     /locus_tag="JN334_000449"
     CDS             complement(18944..20071)
                     /gene="tgt"
                     /locus_tag="JN334_000449"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAY7436714.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20126..21196)
                     /gene="queA"
                     /locus_tag="JN334_000450"
     CDS             complement(20126..21196)
                     /gene="queA"
                     /locus_tag="JN334_000450"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAY7436715.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21289..21862
                     /gene="acpH"
                     /locus_tag="JN334_000451"
                     /pseudo
     CDS             21289..21862
                     /gene="acpH"
                     /locus_tag="JN334_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(21867..23684)
                     /gene="malZ"
                     /locus_tag="JN334_000452"
     CDS             complement(21867..23684)
                     /gene="malZ"
                     /locus_tag="JN334_000452"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAY7436716.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(23840..25213)
                     /gene="proY"
                     /locus_tag="JN334_000453"
     CDS             complement(23840..25213)
                     /gene="proY"
                     /locus_tag="JN334_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HAY7436717.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25289..26608)
                     /gene="brnQ"
                     /locus_tag="JN334_000454"
     CDS             complement(25289..26608)
                     /gene="brnQ"
                     /locus_tag="JN334_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HAY7436718.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27015..28310)
                     /gene="phoR"
                     /locus_tag="JN334_000455"
     CDS             complement(27015..28310)
                     /gene="phoR"
                     /locus_tag="JN334_000455"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HAY7436719.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28368..29057)
                     /gene="phoB"
                     /locus_tag="JN334_000456"
     CDS             complement(28368..29057)
                     /gene="phoB"
                     /locus_tag="JN334_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HAY7436720.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29247..30449
                     /gene="sbcD"
                     /locus_tag="JN334_000457"
     CDS             29247..30449
                     /gene="sbcD"
                     /locus_tag="JN334_000457"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HAY7436721.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            30446..33589
                     /gene="sbcC"
                     /locus_tag="JN334_000458"
     CDS             30446..33589
                     /gene="sbcC"
                     /locus_tag="JN334_000458"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAY7436722.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            33715..34896
                     /gene="araJ"
                     /locus_tag="JN334_000459"
     CDS             33715..34896
                     /gene="araJ"
                     /locus_tag="JN334_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HAY7436723.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(35039..35947)
                     /gene="mak"
                     /locus_tag="JN334_000460"
     CDS             complement(35039..35947)
                     /gene="mak"
                     /locus_tag="JN334_000460"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HAY7436724.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            36072..36983
                     /gene="rdgC"
                     /locus_tag="JN334_000461"
     CDS             36072..36983
                     /gene="rdgC"
                     /locus_tag="JN334_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAY7436725.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37061..37516)
                     /locus_tag="JN334_000462"
     CDS             complement(37061..37516)
                     /locus_tag="JN334_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436726.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37513..37848)
                     /locus_tag="JN334_000463"
                     /pseudo
     CDS             complement(37513..37848)
                     /locus_tag="JN334_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(38003..38287)
                     /gene="ppnP"
                     /locus_tag="JN334_000464"
     CDS             complement(38003..38287)
                     /gene="ppnP"
                     /locus_tag="JN334_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAY7436727.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(38359..39036)
                     /locus_tag="JN334_000465"
     CDS             complement(38359..39036)
                     /locus_tag="JN334_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HAY7436728.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(39294..39485)
                     /gene="yaiA"
                     /locus_tag="JN334_000466"
     CDS             complement(39294..39485)
                     /gene="yaiA"
                     /locus_tag="JN334_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HAY7436729.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(39535..40059)
                     /gene="aroL"
                     /locus_tag="JN334_000467"
     CDS             complement(39535..40059)
                     /gene="aroL"
                     /locus_tag="JN334_000467"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HAY7436730.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(40242..40700)
                     /locus_tag="JN334_000468"
     CDS             complement(40242..40700)
                     /locus_tag="JN334_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAY7436731.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            40820..41629
                     /gene="proC"
                     /locus_tag="JN334_000469"
     CDS             40820..41629
                     /gene="proC"
                     /locus_tag="JN334_000469"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAY7436732.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(41646..42761)
                     /gene="adrA"
                     /locus_tag="JN334_000470"
                     /gene_synonym="dgcC"
     CDS             complement(41646..42761)
                     /gene="adrA"
                     /locus_tag="JN334_000470"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HAY7436733.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        10830 a        11415 c        10664 g         9945 t
ORIGIN      
        1 ccgataaggc gctgaatatg tttgataaag ctattgctgt agatagcagt cgtgaatccc
       61 attatagtga tgagtatttg aaactcatga atatctacgc cgccaatggc aacaaagaaa
      121 aagcggtggt tttatctcaa cgtctacaca aagccattac cgaatctcaa tcgaattact
      181 taagtactct ggaaggaatt ttattgtttt atagaaataa tgatatgcag caagactatc
      241 aaaacagcct ttcaaattac atagtattta tcgataaaat ttttgccttt tccccatcaa
      301 caagaagtga attaaacctc attaatttat taaattcatt aaatgaggtg gaattgatga
      361 gaaaacgtct ggcgtttctg atgacagcac cagaatacgc ttgttataat agtcagtatt
      421 gttatgaaga caaagtcagc gcattaaaaa tcctgtatcg tcatcatgac aatgagctaa
      481 tggacaaata tttcacgact cttcttaaag agatcagtaa tcaaccatta gaagactggg
      541 atataataat aagtaatact ggtgaaaaat ttagtgaagt gggtctaacc aaatatgctc
      601 aacatctcgc atctttcgct gaagaatttt atataaaaga gcaaaaatca cattctgaaa
      661 gtgaaatgag aggactattt tcatcacttg ccgaattata tagttttggt aatgatactg
      721 ccagcgcaaa ccgagtgttc catcagcatg tccctgcctt caatacagat catatgattc
      781 aatattttat aaatgccaaa gaatggaaca atgccaggga attattgata aaagaggaaa
      841 tgttgggtat gcataatctg atattgttgg agaatatttg tatgcaaaaa aatgcagaat
      901 gcatggcaca cattaccttt acgcttaata aattaacaac acaaccagca ataacaaaaa
      961 tagatagcgt gggtaatgag caactttatc agattggcaa aatctaccat aagttggaaa
     1021 ttaaacctga gccagagcaa caggttttaa ttcagtcgct ttatgaccgg gcatcaggaa
     1081 gcgcctctgc aactcaataa atgcatggtc aaaagggcca ccacggtggc cctgacaatc
     1141 agtagctata tccagaaagt taatcgcgga agtttttgaa ctggaacggc tgaccgagat
     1201 cgccaccgcg caccatcgcc atgacagcct gcaaatcatc acgagatttg ccagtcacgc
     1261 ggatctcatc gccctgaatt tgcgcctgca ctttcagttt gctgtctttg atcatcttga
     1321 cgattttttt ctgagtggcg ctctcaatgc cctgtttcag tttcgcttcc acaaaccagg
     1381 ttttaccgct atgaacgata ttttccggta catccagtga actgccttca atgccgcgct
     1441 tcagcagctt ggcacgcaga atatccagca actgattgac ctggaagtcg gactcgctca
     1501 acactttgat ggttttgctg gcgtcgttca gctcaaatga ggcttcaacg ttacggaagt
     1561 caaaacggga ctccacttcg cgactcgcgt tatcgaccgc gttacgtgct tcctgaagat
     1621 caacttcaga gacaatatcg aaagatggca tcttttcctc tcccttcatt tttgatgcga
     1681 agcataatac ctgcaaagtt aaataaccaa cagtcctgca ccaggacgta tcccttaatt
     1741 gtgcatagtc gcaacagcat gcacaattaa gggatagcct tattgaacac ccggagtggt
     1801 tgcgggtgag gaggaacaat gaaaattacc gtattgggat gcggtgcctt agggcaatta
     1861 tggcttacag cactttgtaa acagggtcat gaagttcagg gctggctgcg tgtaccgcaa
     1921 ccttattgta gtgtgaatct ggttgagaca gatggttcga tatttaacga atcgctgacc
     1981 gccaacgatc ccgattttct cgccaccagc gatctgctcc tggtgacgct gaaagcatgg
     2041 caggtttccg atgccgtcaa aagcctcgca tccacactgc ctgtaactac gccaatactg
     2101 ttaattcaca acggcatggg caccatcgaa gaattgcaaa acattcagca gccattactg
     2161 atgggcacca ccacccatgc cgcccgccgc gacggcaatg tcattattca tgtggcaaac
     2221 ggaatcacgc atatcggtcc ggcacggcaa caggatggcg attacagtta tctggcggat
     2281 attttgcaaa ccgtgttgcc tgacgttgcc tggcataaca atattcgcgc cgagctgtgg
     2341 cgcaagctgg ccgtcaactg cgtgattaat ccactgactg ccatctggaa ttgcccgaac
     2401 ggtgaattac gtcatcatcc gcaagaaatt atgcaaatat gcgaagaagt cgcggcagtg
     2461 atcgaacgcg aagggcatca tacttcagcg gaagatttgc gtgattacgt gatgcaggtg
     2521 attgatgcca cagcagaaaa tatctcgtcg atgttgcagg atatccgcgc gctgcgccac
     2581 accgaaatcg actatatcaa tggttttctc ttacgccgcg cccgcgcgca tgggattgcc
     2641 gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa aggagagtga atatgagcgc
     2701 atcggcactg gtttgcctcg cccctggtag tgaagagact gaagccgtca ccacgatcaa
     2761 cctcttggtt cgcggcggta tcaaagtcac cactgccagc gtcgacagcg atggcaacct
     2821 ggcaattacc tgctcgcgcg gcgtgaagct gctggcggat gcgccgctgg tcgaagtggc
     2881 tgatggcgaa tatgacgtga tcgtgctgcc tggtggcatt aaaggcacgg agtgttttcg
     2941 cgatagcact ctgctggttg aaaccgttaa acagttccac cgttccgggc gtatcgtcgc
     3001 ggctatttgc gccgcgccag ccaccgtgct ggtgccgcac gatatctttc cgattggcaa
     3061 tatgaccggc ttcccgacgc tgaaagacaa aattcccgct gaacaatggc aggacaagcg
     3121 cgtcgtctgg gatgcacggg taaaattgct gaccagccag gggccgggta cagctatcga
     3181 ctttggtctg aaaattatcg acctgctggt tgggcgcgag aaagcccatg aagtggcatc
     3241 acaactggtg atggcggcag ggatttataa ttattacgag tagtgtcgga tgcggcaaac
     3301 gtcgcatctg accagatgcg acgtaaaaac tacaaattac gggcgatata ccttcacatt
     3361 gttaaagcct tgctcgcgca gatagagcgc ctgcagacgg ctcatcaccc cgcgctcaca
     3421 ccacagcagc caggttttgt tctggtcgag atcgccaaat ttggtgctca gtttatagaa
     3481 cggcagagaa accacatcaa tcccttcgac tttcagtggc ttatcttcct gttcatcgac
     3541 agaacggata tcgagaatca cgtcgttcgg gccgaagccg ttgacggttt ccacttccac
     3601 cacttcctgc tcggtctgct gggcgatttc gcggatatca acgttattcg cttcctcaac
     3661 cactttatcg agaatactga agtcgaactt ctcttcttcc gcttcaatct tcgatttaac
     3721 cgctttcacc gtcgggcttt tggagatcac gccacaatat tccggcatcg tgcgagcaaa
     3781 gtcttcggtg ccaatctggc gagccaggtt gatgatgtgc tctttgtcgt aagaaatcag
     3841 cggacgcagg atcagcgtgt cagagacgtt atcaatcagg cgcaggttgg tcagcgtctg
     3901 actggacacc tggccgagcg cttcgccggt gaccagcgcc tgtacgccgt aacgttcagc
     3961 cactttagat gcggcacgca ccatcatacg tttgaggata acgcccatct gaccgtcgtc
     4021 gattttctcg agaatttccc cgacaaccgg ttcgaaatta atggcgacaa aacgcacgcg
     4081 gtgggagctg ccaaagcggt tccacagata atgcgccacc tgacgcacgc caatttcatg
     4141 cgctgcgccg ccgaggttaa agaagcagta atgcacgcgg cagccgcgac gcatcaacat
     4201 ataactggaa acaccggagt cgaaaccacc ggaaatgagc gacagcacat cttcctgggt
     4261 gccaatcggg aaaccaccaa taccttcgta gcgaccttta atcagcagga ggcgatcgtc
     4321 ttccacttcc agatgaacag ttacatccgg gttagtcagc ttcacgcgcg cggattcaat
     4381 atgctgattt aaaccgccgc cgacgtaacg ttccacatca atcgagctaa aatcatgttt
     4441 gccacggcgc ttcacgcgta cgcagaaggt tttgccttcc agctgatcgc gatactgaac
     4501 caacgctttc tcaaaaatat cgtgcatgtc ggtaaacggc acgtcttcga cttcgagaat
     4561 atggtggata cccggaatac gggtcagggc gtcgcgaata gtcagacgct ggttttcatc
     4621 tttcgcgcga acttcgatgt tatcccagtg gcggacgaca gcgagcgtct catcatagtg
     4681 ctttaaaacg ttacgaatgt tcccggtaag gatttttata aagcgcaagc gcacagattg
     4741 gcttttgatg gtgatttccg ggaacaattt aatgataaac ttcatggcgg caatggttcg
     4801 ttggcaagcc ttaagcgact tgtataggga aaaatacagc agcccacacc tgcggctgca
     4861 tccaggcgcg gaagtatacc actaacatcg ctttgctgcg cacatcacct taccattgcg
     4921 cgttatttgc tatttgccct gagtccgtta ccatgacggg gcgaaaaata ttgagagtca
     4981 gacattcatt atgccgaaga aaaatgaggc gcccgccagc tttgaaaagg cgctgagcga
     5041 gctggaacag attgtaaccc gtctggaaag tggcgacctg ccgctggaag aggcgctgaa
     5101 cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag gccaaattac aacaagccga
     5161 acagcgcgta caaattctgc tgtctgacaa tgaagacgcc tctctaaccc cttttacacc
     5221 ggacaatgag taatggactt tccgcagcaa ctcgaagcct gcgttaagca ggccaaccag
     5281 gcgctgagcc gttttatcgc cccactgccc tttcagaaca ctcccgtggt cgaaaccatg
     5341 cagtatggcg cattattagg tggtaagcgc ctgcgacctt tcctggttta tgccaccggt
     5401 catatgtttg gcgttagcac aaacacgctg gacgcacccg ctgctgccgt agagtgtatc
     5461 cacgcttact cattaattca tgatgattta ccggcgatgg atgatgacga tctgcgtcgc
     5521 ggtttgccga cctgccatgt gaagtttggc gaagcaaacg cgattctcgc tggcgacgct
     5581 ttacaaacgc tggcgttctc gattctaagc gatgccaata tgccggaagt gtcggatcgc
     5641 gacagaattt cgatgatttc tgaactggcg agcgccagcg gtattgccgg aatgtgcggt
     5701 ggtcaggcat tagatttaga cgcggaaggc aaacacgtac ctctggacgc gcttgagcgt
     5761 attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg ttcgccttgg tgcattaagc
     5821 gccggagata aagggcgtcg tgctctgcca gtactcgaca agtacgcaga gagcatcggc
     5881 cttgccttcc aggttcagga tgacatcctg gatgtggtag gagatactgc aacgttggga
     5941 aaacgccagg gtgccgacca gcaacttggt aaaagtacct accctgcact tctgggtctt
     6001 gagcaagccc ggaagaaagc ccgggatctg atcgacgatg cccgtcaggc gctgaaacaa
     6061 ctggctgaac agtcactcga tacctcggca ctggaagcgc tagcggacta catcatccag
     6121 cgtaataaat aaacaataag tattaatagg cccctgatga gttttgatat tgccaaatac
     6181 ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa agagagttta
     6241 ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg ttccagcggg
     6301 cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta tgtctacaac
     6361 accccatttg accaattgat ttgggatgtg ggacatcagg cttatccgca taaaattttg
     6421 accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca cccgttcccg
     6481 tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac ctccatcagt
     6541 gccggaattg gtattgcggt tgctgccgag aaagaaggca aaaatcgccg caccgtctgt
     6601 gtgattggcg acggcgcgat taccgctggc atggcgtttg aagcgatgaa tcacgcgggc
     6661 gatattcgtc ctgatatgct ggtggtcctc aacgacaatg aaatgtcgat ttccgaaaat
     6721 gttggcgcgc ttaacaacca tctggctcag ctgctttccg gtaagcttta ctcttcactg
     6781 cgcgaaggcg ggaaaaaagt tttctctggc gtgccgccca ttaaagagct gctcaaacgt
     6841 accgaagaac atattaaagg catggtggtg cctggcacgt tgtttgaaga gctgggattt
     6901 aactacatcg gcccggtgga cggtcacgat gtgctggggc ttatcaccac gctaaagaac
     6961 atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg tcgtggttat
     7021 gaaccggcag aaaaagaccc gatcactttc cacgccgtgc ctaaatttga tccctccagc
     7081 ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt tggcgactgg
     7141 ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc gatgcgtgaa
     7201 ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gttacttcga cgtggctatt
     7261 gccgagcaac acgcagtgac ctttgccgcc ggtctggcga ttggtggata caaacccatt
     7321 gtcgcgatct actccacctt cctgcaacgc gcctatgatc aggtgctgca tgacgtggca
     7381 attcaaaagc taccggtcct gttcgccatt gaccgcgcgg gcattgttgg tgctgacggt
     7441 caaactcacc agggcgcttt tgatctctct tacctgcgct gcataccaga aatggtcatt
     7501 atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta tcactataac
     7561 gatggcccgt ccgcggtgcg ctacccgcgc ggcaacgcgg ttggcgtgga actgacgccg
     7621 ctggaaaaac taccaattgg caaaggcatt gtgaagcgtc gtggcgagaa actggtgatc
     7681 cttaactttg gtacgctgat gccagaaacg gccaaagtcg ccgaatcgct gaacgctacg
     7741 ctggtcgata tgcgttttgt gaaaccactt gatgaagcgt taattctgga aatggccgcc
     7801 agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc aggtagcgga
     7861 gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg tgctgaacat tggcctgccg
     7921 gacttcttta ttccgcaagg aactcaggaa gaaatgcgcg ccgaactcgg cctcgatgcc
     7981 gccggtatgg aagccaaaat caaggcctgg ctggcataat ccctactcca ctcctgctat
     8041 gcttaagaaa ttattcatgg caggagtggc agcatgcaat acaacccctt aggaaaaacc
     8101 gaccttcgcg tttcccgact ttgcctcggc tgtatgacct ttggcgagcc agatcgcggt
     8161 aatcacgcat ggacactgcc ggaagagagc agccgtccca taattaaacg cgcgctggaa
     8221 ggtggcataa atttctttga taccgccaat agctattccg atggtagcag cgaagagatc
     8281 gttggtcgcg cactgcggga tttcgcccgt cgtgaagacg tagtcgttgc caccaaagtg
     8341 ttccatcgcg ttggtgattt accggaagga ttatcccgtg cacaaatttt gcgctctatc
     8401 aacgacagcc tgcgacgtct cggcatggat tatgtcgata tcctgcaaat tcatcgctgg
     8461 gattacaaca cgccgatcga agagacgctg gaagccctga acgacgtggt aaaagccggg
     8521 aaagcgcgtt atatcggcgc gtcatccatg cacgcttcgc agtttgctca ggcgctggaa
     8581 ctacaaaaac agcactgctg ggcgcagttt gtcagtatgc aggatcacta taatctgatt
     8641 tatcgcgaag aagagcgcga gatgctgcct ctgtgttatc aggagggcgt ggcggtgatt
     8701 ccgtggagcc cgctggcgcg ggggcgactg acgcgtccat ggggagaaac taccgcacga
     8761 ctggtgtctg atgaggtggg gaaaaatctc tataaagaaa gcgatgaaaa tgacgcgcag
     8821 atcgcagagc ggttaacggg cgtcagtgaa gaactcggtg caacacgagc acaagttgcg
     8881 ctggcctggt tgttaagtaa accgggcatt gccgcaccga ttatcggaac ttcgcgggaa
     8941 gaacagcttg atgagctgct gaacgcggtg gatatcactt tgaagccata acagattgcc
     9001 gagctggaaa cgccgtataa accgcatgcg gtcgtaggat ttaaataaga gtccaggcct
     9061 gatgagacgt gacaagcgtc acatcaggca tcggtgcaca actacgacag aatacccagc
     9121 ggccagtgat gaccgataaa gtacaggatg cctgcggaaa tcaccccggc gacaatatcg
     9181 tcgatcatga tccccatgcc gccatgtaca ttgcgatcaa accagcggat cggccacggc
     9241 ttccacatat ccagaatacg gaaaatcaca aacccggcgg caacccactg ccagtcattg
     9301 gtcggcagcg ccatgagcgt gatccacata ccaataaatt cgtcccagac aatgctgccg
     9361 tgatcgtgca cgcccatatc tttcgccgtt tgatgacaaa gatagacgcc gatacagatc
     9421 cccaacatca ccaccagcga gtagagctgc cagggtaaaa aggtcatcag ataccagaaa
     9481 gggatcgccg ccagcgatcc catcgagcca ggaacgattg ggcttaatcc acttccgaat
     9541 ccgacagcaa gcagatgcca cggattactc atcttcaggc gacttttcgc gacattttta
     9601 tggcgtgaca aaatggtcat atcctttcca gtcaaacgtg acaggttcgc cgtcacgaat
     9661 aaaacaaagc ccttcgatat cggcggtcat ttgcccgata caggtaaacg gtacgccaag
     9721 gtgtccgaga gcaacatcca gcgcgccacg gttcagttcc ggcaccgtga aacacaactc
     9781 gtaatcttca ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa catggcgaga
     9841 aagcgcatca gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt cgctggcttt
     9901 cacgatatgc ccgagatcgg aaatcagacc gtcagagaga tcgatagctg aatttgccag
     9961 atcgcgcagt gcctgcccct gtaaaatacg cggcgatgga cggagatgac gtttgatcaa
    10021 gtagtcagca tctttagcat cggcaacctg caaacggttt tgcaaaatcg ccagcccggc
    10081 ggcgctatcg cccggtgtac cggtcacata gatccagtca cccggtttcg ccccagagcg
    10141 cgttaaggct cgtcccatcg gaacaaagcc gtggataccc aacgtcattg ataatggccc
    10201 acgcgtggta tcgccgccaa tgagttgcat atcgtaataa ttgagaagat caaacaaact
    10261 gtcgctgaag gactcaagcc acgcttcgtc tacgtccggt aaggttaatg ccagcgtcag
    10321 ccaggccgga tcggccccca tcgctgccag atcgcttagg ttcaccgcca gtgctttata
    10381 agccagatca gcaggatcga tatcagggag gaaatggttg cccgccacca gcgtatcagt
    10441 gctgatcgcc agggtctgtt tctcggggat attgagaaga gcgcaatcgt cgccgatgcc
    10501 cagttcgaca tcaagacgag aacttcttac acggtcaaaa taacgggcaa tcagggagaa
    10561 ctcgccacat gccatacgtt atgcctcagc aggtaaaaaa gaaaaggccg gcgatcatgg
    10621 aacagtcttc cgtgaatcta ccggcctgga tattactttt tgttagggcg aatcacaggt
    10681 gctgctttat cgagtacgcc gttgacgaac ttatggctgt cttctgcgcc gaacgatttc
    10741 gccagttcga tcgcttcgtt aatggccact ttgtatggca catcactacg tttagacagt
    10801 tcgtacagcg caatgcgcag tactgctttt tctacctggc ccagttcttc cagcaggcgg
    10861 gacaggtagg gcttcatcag tccgtcgagg tatgcggtgt tagtcgccac cccggccagc
    10921 agctcacgga agtacaggac gtcaacgtct tttacatcct gttcagccag gaactggtat
    10981 tcaacatcag cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg gacggcacac
    11041 tcacgagcgc ggcgacgagc agcaggtttc acggatttcc ccttactaat ttcaggcctt
    11101 gatggctttc aatacattaa tcatttcaag cgcggtcagt gcagcttctg cacctttgtt
    11161 gccagctttg gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg tcagaacccc
    11221 aaaagcaacc ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc ttgcaccacc
    11281 agcgacatat tcaaagtggg cagtgccacc acgaataacc gtacccagcg caatcaccgc
    11341 gtcgtattta ccggttttag ccagtgcacc cgccgccagc ggcagctcat aggcaccagg
    11401 cacccaaaca acggtaatgt tttcatcttt tacctgaccg atacgtttca gtgcgtcaat
    11461 tgcaccttcc agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg tgatggcgac
    11521 gcgagcgtcc ggggtagcaa cgttagcttc aataatgttc attttcttcc ttcgggttcg
    11581 agtatggccc cgcagggggg cggattttag cataatattt cgtgcgctgc ttccctttcg
    11641 agccgggaga tcatgcaccc actaaatgca ggcaaacatc cgggcctaca tgacgtatct
    11701 ctttgaattt aaattggggg gcgtcggcta atttctcaag ccctggcagc gagcataatc
    11761 cacgggcgtc gctgcctaat agtttaggtg cgatatagac aatcagctca tcgactaaac
    11821 ccgcctgtag caatacgcca gcgagcgttg gccccgcttc cacccagatg ctgttaatct
    11881 gctgtttacc cagttgcatc atcagtacaa ccagatccag atgaccttta tgctctggaa
    11941 tcagcaaggt acgcaccgtt tccggccact cacgagaatc ttcctgcgta cgcgcgaacc
    12001 aggtttcgcc gggctgctgc acaatgcgat gttccggcgt cacgcgattt tggctatcaa
    12061 tcacaatacg tactggctgg cggagatttt gttgcgggta gagcgcctga gtttgttcat
    12121 ccagttcaga ccaacgcact gttaaggctg gatcatccgc cagcaccgtg gcgctgctgg
    12181 ttaaaatggc atgactttgt gcgcgctgtc gttgtacgtc gcgccgcgcc tgaggcgaag
    12241 tgatccactg gctttcgccg ctcgccatcg ccgtgcggcc atcaagcgat gcgccaagtt
    12301 ttaactgaat ataaggaaag ccggtgcgca tccgcttgag aaagccttta ttcaattgct
    12361 cggcttcact catcatcagc ccgtggctga cgtcaatgcc agcctgttgc agacggtaaa
    12421 gtccacgccc agcgacctgc gggttaggat cttgcatcgc tgcgaccacg cgcgctacgc
    12481 cagcggcgat gagtgcgtca cagcacggtg gcgtacggcc atgatggcta cagggttcga
    12541 gtgtcacata cgccgttgcg cctttggctt tttcacccgc catacgcaac gcgtgtactt
    12601 cggcatgtgg ttcacccgct cgttggtggt aaccttcacc gacaatttcg ccatctttga
    12661 caatgacgca cccgacattc gggttggggt gggtggtaaa acgtcctcgt tgcgccagct
    12721 ttagcgcccg cgccatgtaa tactcgtcct gcacggctta gtcctccagg cgcgcgatct
    12781 cttcgccaaa ttctttgata tcttcgaaac tgcggtagac agaggcaaaa cggatatagg
    12841 cgactttatc gagctttttc aattgctcca tcaccagatt gccaatcatc ttgctcggta
    12901 cttcgcgctc accggtggcg cgcagctgcg atttaatatg attgattgcc atttcaacgt
    12961 aatcggaact caccggacgt ttttccagcg cccgcagcat tccgctacgc aatttctctt
    13021 cattaaacgg ttcacgcacg tcgttgcttt ttacaacacg cggcataacc agctccgcca
    13081 cttcaaaggt ggtgaaacgt tcattacaca ccagacactg ccgacggcgg cgtacggatg
    13141 aaccctcgcc cacgagacga gagtcaatta ccttagtgtc cacggcgaaa cagaatgggc
    13201 aatgcatacg gcgtcctgac caggtggtta acggaaatct attttaccct gaactgacga
    13261 tacaacaaag gcgcaacgct tttgagataa aggatcgatg ctttcgctgc ttttaccctc
    13321 taccattagg ctaattgcga cgagaaaggg acgacctatg aatactaacg tttttcgact
    13381 gctcctgctg ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg aagtgcgaca
    13441 gatgaaacac agcgtcagca cgctgaacca ggagatgacg caactcaatc aagaaacagt
    13501 caaaatcact cagcaaaaca ggctgaatgc aaaatccagc agtggggttt accttctgcc
    13561 tggagcgaaa acaccggcaa gactggaaag ccagatcggt actttacgta tgtcgctggt
    13621 gaatattacg cctgatacag atggcaccac ccttacacta cgtattcagg gtgagtcgaa
    13681 tgatcccttg cccgcattca gcggaactgt tgaatatggg caaattcagg gaacaataga
    13741 caactttcag gaaatcaatg tgcagaatca attgattaat gcccctgcca gcgtcctcgc
    13801 ccccagcgat gttgatattc cgttacaatt aaaggggatt tctgtggatc agttaggctt
    13861 tgtacgtatc cacgacattc aacctgttat gcagtaaacg tatttcggga cgattttgtg
    13921 cgtcccgcaa catcttcccc gtcattttgt tactctgctt acatcacctg gattgatagt
    13981 aaaagtttgc aacaagggcg aaagtcagta caatccccgc ccgaatgtgt gtaaacgtga
    14041 acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa catatttttg tgagcaatga
    14101 tttttataat aggctcctct gtatacgaaa tatttagaaa cgcaatttgc gcctttttca
    14161 ctcccgcaag ggattttcaa acagtggcat acatatgaaa aaaacattac tggcagccgg
    14221 tgcggtactg gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg acaaaccgca
    14281 gtatctttcc gactggtggc accagagcgt taacgttgtc ggaagctatc acacccgttt
    14341 cggaccgcag atccgcaacg atacctacct tgagtacgaa gcattcgcta aaaaagactg
    14401 gttcgacttc tatggttatg cggatgcgcc ggtattcttc ggcggtaact ccgatgctaa
    14461 aggtatctgg aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt tctccatcga
    14521 caagctgacc aatactgacc ttagcttcgg tccgttcaaa gagtggtact tcgcgaacaa
    14581 ctacatttac gacatgggtc gtaataaaga tggtcgccag agcacctggt acatgggtct
    14641 gggtaccgat atcgacactg gcctgccgat gagcctgtcc atgaacgtct atgcgaaata
    14701 ccagtggcag aactatggcg cagcgaacga aaacgagtgg gacggttacc gtttcaaaat
    14761 taaatacttt gtgccgatta ccgatctgtg gggcggtcag ctgagctaca tcggcttcac
    14821 caacttcgac tggggttccg atttagggga tgacagcggt aacgcaatca acggtattaa
    14881 gacccgtact aataactcta tcgcttccag ccatattctg gctctgaact acgatcactg
    14941 gcactactct gtcgtagctc gttactggca cgacggtggt cagtggaacg acgatgcaga
    15001 actgaacttc ggcaacggca acttcaacgt tcgctctacc ggctggggtg gttacctggt
    15061 agtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat ttctgattgt
    15121 taacaacgtg tgctttgttc atgccggatg cggcgtaaac gctttgtccg gcccacaaaa
    15181 gcacgtaaat tcaatatatt gctgaaataa cgtaggcctg ataagcgtag cgcatcaggc
    15241 aattttacgt tttaatcact tcttcttgtt catcatcgct ttcaggtcag cgaatgggtt
    15301 gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg tcgtaccata
    15361 ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac acaacaattc
    15421 ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt gaacggtaag
    15481 ttcacgcagg ttagaataaa caaactcgcg ggaacagcga ccgcagaccc acggatagat
    15541 ttttaatgct ttttcgcgat agccgctttc taaccgtgca tagttttttg ggatgatagc
    15601 catgaaaagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga tgcggggatt
    15661 ttcgcaatct ccatacatca aaaaaaatcc cgatcttctg accgggattt tcaacatcaa
    15721 cgggaacttg attaaggcag aattgacggc tgatccgccc cttctttttc cactttctgc
    15781 tgcaacatgt gttcgcgctt catacccagt ttcagagcca acgcagatgc tacgtagatg
    15841 gaagatgcag tgccgatgga aacaccgata agcatggtca gcgagaagcc ttccagtacc
    15901 ggaccaccga agagatacag catcaggata accatcaagg tagtaccgga tgtgatcaag
    15961 gtacggtgca gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt accgcgacgg
    16021 atcttgcgga agttttcacg aattcggtcc gataccacga tactgtcgtt aagcgagtaa
    16081 ccgataaccg acatcaacga tgccacaatg gtcaggtcaa tctcgatatg gaataacgac
    16141 aaaataccca gcgtaatgat aacgtcgtgc gtcagcgcaa taaccacccc tgccgccagt
    16201 cgccactcaa agcggaaacc tacgtacacg aggatagaca gcagcgctgc catcaacgcc
    16261 atcgcaccgg tttgcgcaag gtcagccccc acgctcggac cgacgaactc aatacgcttc
    16321 actgctgcat tctgattggt ggattcgtta atcaccttca gaacttggct gcccagcacc
    16381 tgaccgccgg tttcgccttc agcaggcggc atacggacca tgatgtcgtg gctgctacca
    16441 aagttttgca gcattggctc ttcaaaaccg gctttttgca atgcatcacg cattacgtca
    16501 atttcagccg gtttttcgag cgtaatttcg ataaccgtac caccggtgaa atccagcccc
    16561 cagttaaagc cgcgcacgcc cataataacg atagcagcga ttaacagcag accagagatg
    16621 ccgaaagccc agtagtccca gcgcataaag tcatagactt tacggccgtg gtttagttgt
    16681 tcaacagtat attcctgtgc cacatcgcac tcctcagatt gacagcttct tgacgcgctt
    16741 gccgccatat agcaggttta cgatggcacg cgtaccgaca atcgcggtaa acatcgacgt
    16801 cgccacaccg ataccggtag taatcgcgaa ccctttaatt gccccggtac ccactgcgta
    16861 caggatgatg actttaatca gcgtggtgat gttcgcatcg aagatagaac tgaatgcgcc
    16921 acgataacct tcatcaattg cctgttgaac agtacgtccg ttgctcaact cttctttaat
    16981 acgttcgttg atcagtacgt tcgcatcgac cgctaccgca agggttaaga cgatacccgc
    17041 gatacctggc atactcagtg ttgcgcctgg caacagcgac ataataccga cgatcaagat
    17101 caagttggca atcagagcac tggtcgcaat cagaccaaac ttcttataga agatgatcat
    17161 gaacagaata gaaaccagca gaccggcaag gcaagcctcc agcccctgct caatgttctg
    17221 catacccagg gtcgggccaa ttgtacgttc ttcaacaatc tgaatcggcg cgatcaacgc
    17281 accggcacgc agcagcagtg acagctgacg ggcttcgttc gggttgttga tgccggtgat
    17341 acggaagctg ttacccagac gagactggat gttggcgatg ttaatcacct cttcctgttt
    17401 caccagaacc gcacgaccat ttgcatcttt cttaccgctg tctttgtact ccacaaacag
    17461 ggttgccatc ggtttgccga tgttgtcctt ggtgaagtta gacataatgt tgccaccagc
    17521 gctatcgagc gagatgttaa cctgcggctg gttgtattcg tcctggctgg aagtggagtc
    17581 ggtgatatgg tcaccagtca gaattacgcg tttgtacagc acaaccggct gaccttcgcg
    17641 ggtctgtttc acttcagagt cgcccggtac gcgaccggat gccgcagcgg cctgatcaac
    17701 attggtgttt accagacgga attccagcgt tgccgtcgcg cccagaatct ctttcgcacg
    17761 cgcagtgtcc tgaataccgg gcagttcaac aacgatacgg tcagcaccct gacgctgaac
    17821 caccggctcc gccacgccaa gttggtttac acggttacgc aggatattaa tgttctgctg
    17881 caccgcatat tcacgcgctt cactcagacg agcatcgctc attaccgcac gcagctggtt
    17941 gctgccctgg ctgctaatca ccaggtccgg atggcgcttg ctcagatacg caatggcttc
    18001 atcacgagct ttagcatcgc ggaaagtgat gctcaggccg tagttgtttt ctttacgaac
    18061 agtggtatac gggatgccct tttcgcgcag gtcactgcgt aggctatcga tattttgttc
    18121 ctggagtttg ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa cgccgccacg
    18181 caggtcaagg ccgagcttca tcggctcagc atgaatagct gccagccagc gcggcgttgc
    18241 cggggcaagg ttaagcgcca cgacgtattt gtcacccata acgcccatta atgcttcacg
    18301 agcgcgcaac tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct cttccagtgc
    18361 cacagactta gcagttattt tttcttcttg taacgttttc tggacctgga tcagcgtttg
    18421 ctcactggcg gcgactccgc gcgcaccagt gatctgaaca gccggatcct caccaaacag
    18481 gttgggaagc gcatacagca gaccgatgac aatcaccacg atcagcatga cgtacttcca
    18541 caaaggataa cggtttaaca cggcaattcc cttagggaaa aattttaatt acagcgcctt
    18601 catggtgcct ttcggcagga cggcagctac gaagtcacgt ttaataacta cttcagtggt
    18661 gtcattcagc gcgatagcaa tgtagccgtt ttccgctact ttggttacgc gaccaaccag
    18721 gccaccgttc gtcagaactt catcaccttt ggcaatggag tccatcagct ttttgtgttc
    18781 tttggtgcgc ttctgctgtg gacgcaggat catgaaatag aaaatcagac cgaataccac
    18841 cagcatcaaa atcaaagaca tcgggctacc ttgcgccggt gcacccgttg ccgctaccgc
    18901 atcagaaata aaaaagctca ttaaatttcc ctcattatta atattaatca acgttcaaag
    18961 gtggtacttc tcgcccctga cgctggtaaa aatcagttac gaagctctct aatttaccct
    19021 cttcaatagc cttgcgtaaa cccgccatca aacgctggta gtaacgaagg ttatgaatgg
    19081 tgttgagtcg cgcgcctaat atttcgttgc aacggtcgag atgatgcaag taagcgcgtg
    19141 aataattgcg acaggtgtag caatcacact caggatcgag tgggccagta tcgctcttat
    19201 atttcgcatt gcggattttc accacgccat cggtcacgaa caaatgacca tttcgggcgt
    19261 tgcgggttgg cattacgcag tcaaacatat cgataccgcg acgtacgcct tcaaccaggt
    19321 cttccggttt accaacgccc atcaggtaac gcggtttgtc tgccggaatt tgcggacaca
    19381 catgctccag aatacggtgc atatctgctt tcggctcacc cacagccaga ccgccgacag
    19441 cgtagccatc aaagccgata tctaccagac ctttaacaga tatatcacgt aaatcttcgt
    19501 aaatactgcc ctgaattata ccaaataacg catttttgtt accaagactg tcaaaacgct
    19561 cacggctacg cttcgcccaa cgcagagaca tctccataga gcgttttgcg taatcccagt
    19621 cagcaggata cggcgtacac tcatcaaaga tcatgacgat atccgaacca agatcgtact
    19681 gaatctccat cgacttttcc gggtcgagga aaatcgggtc gccgttgatc gggttacgga
    19741 agtgaacgcc ctgttcggtg attttacgaa tatcaccaag gctgaagacc tggaagccgc
    19801 cggagtcggt aagaatcggt cctttccact gcataaaatc gtgcagatcg ccgtgcagtt
    19861 tcatgatttc ctggcccggg cgcagccaca ggtggaaggt gttgccgagg ataatttgcg
    19921 cgccagtggc ttcaacttct tccggcgtca tcccttttac ggtgccgtag gtgccaacag
    19981 gcataaaaca aggcgtttcc actacgccac gatcaaagac caggcggcca cggcgtgcgc
    20041 gaccgtcggt ggtgtccagt tcaaatttca ttttttctcc actacgtcag aaaaacagtc
    20101 caacgtttaa accagcgccg cggaattact ccccgacgcg ctcattaatt gcctgcggat
    20161 tgtacgtgat aaacatcgca tcaccgtaac taaaaaagcg atatttctct tctaccgccg
    20221 ctttataggc attcatggtg tgttgataac cggcaaaggc cgaaaccagc ataatcagcg
    20281 tcgactctgg caagtggaag ttcgtcacca gcgcatcgac cactttgtac tggaagcccg
    20341 gatagataaa gatttgggta tcgtcaaaga acggttcgat aagatcgttt tttgccgcct
    20401 gtgccgcgct ttccagcgaa cgtactgaag tggtgccaac cgcaatcacc cggttaccgc
    20461 gcgctttcgc cgccagtacc gcgtctacca catcctgcgg tacttcagcg tattccgagt
    20521 gcatgatgtg atcttcaatg gtgtcgacgc gcaccggctg gaaggtgccc gcaccaacgt
    20581 gcaacgtcac aaacgccatc tccacgcctt tggcgcgtaa tttttccagc aaaggctcgt
    20641 caaaatgcag accagcggtc ggggctgcaa ccgcgcccgg tttttcgcta taaacggttt
    20701 gataaagttc gcggtcagcg tcttcgtccg gacgatcgat atacggcggc agcggcatat
    20761 ggccgatgct gttgaggata tccagtaccg agcgttcatc attaaattcg acttcaaaca
    20821 gtgcgccgtg gcgcgcggtc attgttgcgt tgatactttc gtcatcgccc agcaacagtt
    20881 ctgcgccagg ttttggcgct ttcgaggcgc gaatatgcgc aagaatgcgt ttgtcgtcga
    20941 gcatccgctc aaccagcact tcaatcttgc cgccgctggc tttacgccca aacagacgcg
    21001 ccgggatcac gcgggtatta ttaaaaacca gaagatcgcc ggggttgagc ttatcgagta
    21061 aatcggtgaa agtaccgtgc gtcagcgcgc ccgtcggccc gtccagcgac agtaaacgac
    21121 agctactgcg ttcaggcatg ggatagtggg caatcaggga ttcgggcaat tcaaaggaga
    21181 aatcggtaac gcgcatgaca ctgactcaga ctaaaataag agtcgggtag tctagtgccg
    21241 gggcgctctc cctgcaacct ttacccccct ttggatagaa tataatcgat gaatttttta
    21301 gctcacctgc atttagccca tctcgcggaa agctcgcttt ctggcaattt actggctgat
    21361 ttcgtacgcg gaaatcctga agaaagtttt ccgcccgacg tcgtggctgg cattcatatg
    21421 catcgacgta ttgactgaca atctgccgga agtccgcgaa gcacaggagt ggtttcgtag
    21481 tgaaacgcgc cgcgttgcgc ctattacgct ggatgtcatg tgggatcact ttctttcccg
    21541 ccactggtcg cagctgtcgc cggactttcc gctacaggaa tttgtctgtt atgcccgcga
    21601 gcaagtgatg acgattttgc cggactcacc gccacgtttt atcaatctga acaattactt
    21661 gtggtcagag cagtggctgg tgcgctatcg cgatatggat ttcatccaga acgtgttaaa
    21721 cggcatggca agccgccgcc cacgtctgga tgccctgcgt gactcctggt acgatttaga
    21781 cgctcattat gacgccctcg aaacccgctt ctggcagttt tatccgcgga tgatggcgca
    21841 ggcgtcacac aaggcgttat aaaacgttag ttcatccata ccgtagccga aatggcaggc
    21901 aaagccagaa tcccgtcagt cagttgccca tgcccttctt tgcattgcca ttgcacggca
    21961 ttgagaaacg gtgacgcggg tagcaccact tcactggcct cgccacggtt gattgccacc
    22021 agtacacgtt gctgattcag cacgcggaca aataccacta cgttatcttc cgcatacagc
    22081 acctgacagc cgccacgacg cagcgcctga cttttcttac gcagcacaat cattcgccgg
    22141 tacagcgcga ataacgccgt atcctgcttt tccacctgcc aggggaacgg tttacggcaa
    22201 aacggatcgt ttttaccatc cagtcccact tcatcaccgt aataaatgca cggtacacca
    22261 ggccaggtga acagccagac caccgccagc ggcaggcgcg caatatcccg accgagcagc
    22321 gttttaaatc gcgcagtatc gtggctatcg agctgattaa acatacgtaa ttgttgttga
    22381 tgagaaagcc ctgcgcggta attatccatc caggccatac aggtttgggc gtcaatttgc
    22441 tgcggatcat aagagatgtc ggtattggca agaaatcccc acaacgggaa tgtgaagcca
    22501 cgatagttca tggcagcatc ttccacatcg gcctgtaacc attgccgtgc atcgccaaaa
    22561 tgttcgccga caatatacgc ttccggctgg gtttctttcg ccgcttcggt gatcccggca
    22621 acgtgctgca tattattgcg cgccccaccc gcctccccca gcatatgcac cacatccagc
    22681 cgccagccgt ccatattcca cggcgctttc agccagtggc ggacaatact gtcttccccg
    22741 cgataaattt cattcaccag actttccgac tgataatcca gcttcgtcaa gctggcatag
    22801 ccaagccagt cgagcgccgt gccatcatca ctaaacgagt accagtcgcg ccagggcgat
    22861 tcagagttgt gacaagctcc ccccgtgcca agattgtgcc tgtcaaacca ggcatgggaa
    22921 tcgccactgt ggttaaacac gccgtccagc accagccgca ttcccagctg ctgcgtattg
    22981 tgtcgcaaac gcagcaacgc cccatcaccg ccaaactgcg gatcgacatg gcgataatcc
    23041 tcggtatcgt atttatgtac gctgggagct ttaaacaccg gattgagata cagcgctgtc
    23101 acgccaagct ttttcagata cggcagtttt tcgcttatcc cgtccagatc gccgccatag
    23161 aacgttgatc cgcccgcctg cgccgtgacc ggttcatccc agtcacgcaa gatgatctct
    23221 tgtccggctg catgatggta atagacatga tcctgttcag cttcacgagg aagactacgc
    23281 gcaaaacgat cagggaagat ctgataaaaa atctgatccg cagcccattg tgggccgata
    23341 tcaggtacat cgacggcaaa ctgctccagt cgtgccggcg gcattcggct gaagccctgc
    23401 ggtgtaaacc aacgctggcg atcgtgccac agcagtttga aactgtaacg ccgccggggt
    23461 tgtccgctgg agagatcaat cgccgcacgc catgcggtga cgcctggctg cggctgactg
    23521 cgctgcttat gcattggtac tgacatttct tcgttatcgt gttctgtacg cagcataatg
    23581 cgctgcggtg ggtcttcgcc cgtcagccac agtgtaatga gcagttgatc tttgctttgt
    23641 ttaacaaatg ggggcaccgg caggtgccat gcatttaaca tcataattcc cctgtgatga
    23701 aaatctgatc accttgccat agcctaatgc aggatcactc atcatctgaa ggatatttgg
    23761 gggaggagga cgggggcgga taactttcgg aatagccgga tcaggatttc ctcctgaacc
    23821 ggcttacaga acgcaagtat tattggtttt cagccagctg acgatcgtgg cggcgtttaa
    23881 acatccagcc aatcaacagc acaacaatcc acgcgaaacc gacatacagc gagatacgcg
    23941 tatccgggtg ataaccaatc aacccgataa taaagagcag gaaaatcagc ccgccgatgg
    24001 tcgttgctac cccacccggc actttaaatt tcagcgcctt aacttcttct ggcggcaaac
    24061 ggcgacggaa ggcgatttgc gacagcagga tcataatcca cacccatacc gtggcgaagg
    24121 ttgccagcga agcgatcacc aggaagacgt tttccggcat gatgtagttc agatacaccg
    24181 caaacagcag cgcggtagtc atcaccagca ccgtaaccca cggaataccg cgacgcgacg
    24241 ttttgctgaa aattttcggt gcgctgccct gctctgccat accgtggagc atacggccta
    24301 cgccaaatac gtcactgtta attgccgaca gcgaagcggt cagcacaaca aagttaagaa
    24361 tgctggcggc aaaggtaatg cccatatgct ggaacgtcag tacgaacggg ctaccggcag
    24421 tgccaacctg attccacggg tagatagaca taatgacgaa cagcgtaccg acgtagaaca
    24481 ccagaatacg catcggcacg gagttaatcg cacgcggaat cgatttctca ggatctttcg
    24541 cttcaccggc ggtaatcccg ataatttcga tcccaccgta agcaaacatc accatttgca
    24601 acgacattac catgccaagc cagccgttac tgaagaagcc gccgttgctc cacaggttat
    24661 gaataccggt cggttgcccg ccgttgccaa tcccccagat gatgatgccg aaaccggcga
    24721 caatcatgat gatgatggtg gcgactttaa agaacgagaa ccagaattcc agctcaccga
    24781 agaccttcac gctcatcagg tttacggcgc aaatgatcag caccacgctc agtacccaaa
    24841 tccagtgcgg caccgtcggg aacaagacac ccatatagat accaaaagcg gtcacatcgg
    24901 cgatggcgac aataaggatt tcaaaacagt aggtccatcc ggtaatgtaa cctgccagcg
    24961 ggccaaggtt ttcctgcgca taacgcgaga aagagctggc ggccgggtta tgtaccgaca
    25021 tttcccccag cgcacgcata atgatatacg ccgcgatacc accgataata taggccaaca
    25081 acacgctcgg accggccatt ttgatggcgt ctgccgaacc gtaaaacagc ccggtgccaa
    25141 ttgctgaacc cagtgccata aagcgtatgt ggcgggtact tagcccacgc tttagcttat
    25201 tcttactttc catcaattcc taacccatca acacaataaa aaaccacggg gcatcgcagc
    25261 cccgtggtta aaacaaatgt tcagtgattt agtgagcgct ggaggtcacc tgacgacctg
    25321 ccgcgcgatc ccagataatg gccagaacca ccatcaccac tgttggcatt aaccacgcca
    25381 gaccttgttc ggccagcggt aaacgctgcg cccaggacgg taagatatcg ctgaatgcag
    25441 atgctttgat cccgtcgaga ataccaaaaa gcaggctgat aaacatcggc ggagcaatca
    25501 cgcgggacga attatgccac catgagcgtg taaaacttaa tacaaccagt gcgatacacg
    25561 gcggataaat agcggtcagc accggtacgg agatctggat cagctggctt aagccgaggt
    25621 tagacaccac catcgagaag ccgccgagga taaacaccag cgtacgataa gagagcggta
    25681 cgtactgggc aaagaattct gcacaagcac aggtcaggcc aaccgccgtg accagacagg
    25741 cgatgaagat taacgccgcc agcaggaagc taccgccgcc gccaaaggtg tgctgaacgt
    25801 aagcatgcag aatagcagcg ccgtttgcag actgatcgac cagcgacgcg ctgtctgaac
    25861 ccagacagaa cagcgccagg tacagcaaag tcagaccaac acccgccatc aggccagccc
    25921 agacggtata acgggtcagc agacgcgctt cggtaacgcc acgagaacgc gccgcgttaa
    25981 caataacgat accaaacacc attgcgccca gcgtatccat ggtcagatag ccgttaacga
    26041 agccgttaga aaatgcagcg ttttgataag cctcagtcgc cgtgctgata gaacccgccg
    26101 gccagacaat cgcgacaaca gacaggatga ccagcgcgat aattttcagc ggcgcaagga
    26161 agttgcccac ggtatccagc agcttgcccg gatagagcga aaccagaata acgatagcga
    26221 aatagaccag gctgtagata aacagcggca gcgcggaatc acccgtcagc ggcgcaatcc
    26281 ccacttcaaa ggaaacggta gctgtacgcg gcgtagcgaa aagcggcccc accgccaggt
    26341 aacaaaccgt tgccagcagt acgccagcga ctttaccgat tggcgtgctg aggctgtcaa
    26401 caccgccgcc aacttttgcc agcgccacta ccgttaatac cggcaggcca acggcagtaa
    26461 tgaggaagcc gaatgccgca gtccagacgt gttcgcctgc ctgtaagccg accattggag
    26521 ggaaaataat gttacctgcg ccgacgaaca acgcaaatgt cataaagccc agagcgatga
    26581 tatcgcgcga tcttaattga tgggtcataa atcatactgc ctgtggatgt ggtgttgtaa
    26641 cgttaaattt ttgtgccaat ccctggggat gatacagcgg tatatttaaa caattttagc
    26701 gggaaatact cccaaccagg cgcggcgaag aatagttttt cgatttaccg cgttaatgca
    26761 gtcagtatga cagcacctga agcgcaattt aaacgcttat aacgttttaa agcaaggagg
    26821 gatctaaaaa ccagaggtat atagcgataa gaaatcacca accagtataa aacactattt
    26881 ccgtgaaaaa gacatggcta atcatgcgaa caaaaaatca ccaaccgttg taaaatctaa
    26941 cgcccgtatt ttgactgcaa agaaaataag ccagcatcgg ctggcttatg gcaataaaag
    27001 atgacaaagg cggattaatc gctgtttttg gcaattaaac gttccgggat aacaaaactg
    27061 aaacgtgttc cttttcctac tgtactctca atattcaggc gactttcgtg atgattcacc
    27121 gcatgtttca cgatcgctaa ccctaatccg ctaccgccgg tttgccggga acgcgcttta
    27181 tcaacgcgat aaaaacgctc ggtcaggcgc ggaatatgct ccggtgcaat gcccggtccg
    27241 ttatcttcaa cgctaaattc ggcaccgtgc ggcactcgct gccagcgtac ggtgatatgc
    27301 gtgccttccg gcgtatgatt cacggcgtta tagaccaggt tcgaaatcgc actgcgtagc
    27361 tgatcttcgt tgccagacac cttgaggccg ttatctatct caaaggtaaa tgtctgtttt
    27421 ttctgactca gagtctgagc ctcgcgctca acaacgcgca gcatcatcgg cacatcaacc
    27481 ttttcattga gcaattgcgt cggcgcggct tcaattttcg acagcgttag caactgcttc
    27541 accagccctt ccatccgctg ggtctgctcg cgcatggtgt gcaacgcttt ttcgcgtacc
    27601 gcgccttcca gcggctgctc atccatcatc tccaggtaac cctgtaacac ggtcaatggc
    27661 gtacgtaact catggctcac gttggcaaaa aagttacgcc gcgccccttc cagttgatgc
    27721 atttgcgtga catcacgcgc caccatcagc aactgtttgt gggtataagg catgacgcga
    27781 atttccagat gccgcccggt gttgagcacc agattgagcg ggcgagaaaa atcacgcgtt
    27841 ttcagatatt gcgtaaactc cgggtaacgc agtaggttaa ggatgttctg cccgttatct
    27901 tccggccagc gcaaaccaag aatttgttgc gccagaccgt tacaccagaa aataccgccc
    27961 tcttccgtgg tcagcaccac cgcgtcgggc agcgactccg cgccgctacg aaagcgttta
    28021 atcagattgc ccagttcacg gcggcgtttt ttatttcgca gctgcatctg gtgtaagccg
    28081 tatagtagcg gttcccagct accacgcccc ggtggcgggg tcatactgcg atccacccac
    28141 agccaccatg aaaggcgcaa taaattccag aaatgccaga taagcagtcc tgttaccgat
    28201 gccagcaaaa accagggcag gtaaccaaaa aatgcaccca ggatgaaagc cgggaggcag
    28261 caaagtagca gctccagcac cagccttttc cacgacagcc gttccagcac gtaagatact
    28321 ccagttaaga aatcataagc cctgctctgc gtccgatgag caaggcgtta aaagcgggtt
    28381 gaaaaacgat atcctgtacc gcgcacggtc tgcaccatgc ggtcatgccc gccgggctcc
    28441 agtgctttac gcaggcgacg aatgtggaca tcgaccgtgc ggtcttccac atacacgtta
    28501 gttccccaga cgtggtttaa cagctgctcg cggctgtaca cgcgctcagg atgcgtcata
    28561 aaaaagtgca gcagtttaaa ttctgtcggc cccatctcca gcggctcttc gcccgccatc
    28621 actcggtgag atgtcgggtt gagacttaat ccctgcatct taatcacctc ttccaccgcc
    28681 attggcgaaa tacggcgcat taccgctttg attcgcgcca ccagctcctt cggcgaaaat
    28741 ggcttggtga tatagtcatc cgcgccggtt tcaaggccgc gcacgcgatc ttcttcttcc
    28801 cctctggcgg tcaacatcac cactggaata tcccgggtca tcgactcgcg cttgaggtgt
    28861 ttgatgaact ggataccgga gccgccaggt aacatccagt caaggagaat taaatccggc
    28921 cagggttcat tcagttgatt cacagcactg tcataatctt ccgcttcgac cggctgaaag
    28981 ccattttgtt cgagcacgaa gcagaccatt tcgcgaattg gagcttcatc ttctacgacc
    29041 agaatacgtc tcgccatgat ttgccctgtt gtaataaata ggttgcgatc attaatgcga
    29101 cgtcattatg cgtcagattt atgacagatt tatgaaaagc tcgtcgcaca tatcttcagg
    29161 ttattgattt ccgtggcgca gaaaaaagca aatggcacat ctgtttgggt ataatcgcga
    29221 ccatgctttt tcgccaggga accgttatgc gcatccttca cacctcagac tggcatctcg
    29281 gccagaactt ctacagtaaa agccgcgaag ctgaacatca ggcttttctt gactggctgc
    29341 tggagacagc acaaacccat caggtggatg cgattattgt tgctggtgat gttttcgata
    29401 ccggctcgcc gcccagttac gcccgcacgt tatacaaccg ttttgttgtc aatttacagc
    29461 aaactggctg tcatctggtg gtactggcag gaaaccatga ctcggtcgcc acgctgaatg
    29521 aatcgcgcga tatcatggcg ttcctcaata caaccgtggt cgccagcgcc ggacatgcgc
    29581 cgcaaatctt gcctcgtcgc gacgggacgc caggcgcagt gctgtgcccc attccgtttt
    29641 tacgcccgcg tgacattatt tccagccagg cggggcttaa cggtattgaa aaacagcagc
    29701 atttactggc agcgattacc gattattacc aacaacacta tgccgatgcc tgcaaactgc
    29761 gcggcgatca gcctctgccc atcatcgcca cgggacattt aacgaccgtg ggttccagta
    29821 aaagtgacgc cgtgcgtgac atttatattg gcacgctgga cgcgtttccg gcacaaaact
    29881 ttccaccagc cgactacatc gcgctcgggc atattcaccg cgcacagatt attggcggca
    29941 tggaacatgt tcgctattgc ggttccccca ttccactgag ttttgatgaa tgcggtaaga
    30001 gtaaatatgt ccatctggtg acattttcaa acggcaaatt agagagcgtg gaaaacctga
    30061 acgtaccggt aactcaaccc atggcggtgc tgaaaggcga tctggcgtcg attaccgcac
    30121 agctggaaca gtggcgcgat gtatcgcagg agccacctgt ctggctggat atcgaaatca
    30181 ctactgatga gtatctgcat gatattcagc gcaaaatcca ggcattaacc gaatcattgc
    30241 ctgtcgaagt attgctggta cgtcggagtc gtgaacagcg cgagcgtgtg ttagccagcc
    30301 aacagcgtga aaccctcagc gaactcagcg tcgaagaggt gttcaatcgc cgtctggcac
    30361 tggaagacct ggatgaatcg cagcagcaac gtctgcagca tcttttcacc acgacgttgc
    30421 gtaccctcgc cggagaacac gaagcatgaa aattctcagc ctgcgcctga aaaacctgaa
    30481 ctcattaaaa ggcgaatgga agattgattt cacccgcgag ccgttcgcca gcaacgggct
    30541 gtttgccatt accggcccaa ccggtgcggg gaaaaccacc ctgctggacg ccatttgtct
    30601 ggcgctgtat cacgaaactc cgcgtctctc taacgtttca caatcgcaaa atgatctcat
    30661 gacccgcgat accgctgaat gtctggcgga ggtggagttt gaagtgaaag gtgaagcgta
    30721 tcgcgcgttc tggagccaga atcgggcgcg taaccagccg gacggtaatt tgcaggtgcc
    30781 tcgcgtagag ctggcgcgct gcgccgacgg caaaattctc gccgacaaag tgaaagataa
    30841 gctggaactg acagcgacgt taaccgggct ggattacggg cgcttcaccc gttcgatgct
    30901 gctttcgcag gggcaatttg ctgccttcct gaatgccaaa cccaaagaac gcgcggaatt
    30961 gctcgaggag ttaaccggca ctgaaatcta cgggcaaatc tcggcgatgg tttttgagca
    31021 gcacaaatcg gcccgcactg agctggagaa gctgcaagcg caggccagcg gcgtcacgtt
    31081 gctcacgccg gaacaagtgc aatcgctgac agcgagtttg caggtactta ctgacgaaga
    31141 aaaacagtta attaccgcgc agcagcaaga acaacaatcg ctaaactggt taacgcgtca
    31201 ggacgaattg cagcaagaag ccagccgccg tcaacaggcc ttgcaacagg cgttagccga
    31261 agaagaaaaa gcgcaacctc aactggcggc gcttagtctg gcacaaccgg cacgaaatct
    31321 tcgtccacac tgggaacgca tcgcagaaca cagcgcggcg ctggcgcata ttcgccagca
    31381 gattgaagaa gtaaatactc gcttacagag cacaatggcg cttcgcgcga gcattcgcca
    31441 ccacgcggcg aagcagtcag cagaattaca gcagcagcaa caaagcctga atacctggtt
    31501 acaggaacac gaccgcttcc gtcagtggaa caacgaactg gcgggttggc gtgcgcagtt
    31561 ctcccaacaa accagcgatc gcgagcatct gcggcaatgg cagcaacagt taacccatgc
    31621 tgagcaaaaa cttaatgcgc ttgcggcgat cacgttgacg ttaaccgccg atgaagttgc
    31681 taccgccctg gcgcaacatg ccgagcaacg cccactgcgt cagcgcctgg tcgcgctgca
    31741 tgggcagatt gttccccaac aaaaacgtct ggcgcagtta atggtcacta tccagaatgt
    31801 cactctggaa cagacgcaac gtaatgtcgc acttaacgaa atgcgccagc gttataaaga
    31861 aaagacgcag caacttgccg atgtgaaaac catttgcgag caggaagcgc gcatcaaaac
    31921 gctggaagcc cagcgtgcac agttacaggc gggtcagcct tgtccacttt gtggttccac
    31981 cagccacccg gcggtcgagg cgtatcaggc gctggagcct ggtgttaatc agtctcgatt
    32041 actggcgctg gaaaacgaag ttaaaaagct cggcgaagaa ggtgcggcgc tgcgcgggca
    32101 actggatgca ttaacgaagc agcttcagcg cgatgaaaac gaagcgcaaa gcctccgaca
    32161 agatgagcaa gcacttactc aacaatggca agccgtcacg gccagcctca atatcacctt
    32221 gcagccacag gacgatattc aaccgtggct ggatgcacaa gatgagcacg aacgccagct
    32281 gcggttactc agccaacgac atgaattaca agggcagatt gccgcgcata atcagcaaat
    32341 tatccagtat caacagcaaa ttgaacaacg tcagcagcag ctcttaacgg cattggcggg
    32401 ttatgcactg acattgccac aggaagatga agaagagagc tggttggcga cacgtcagca
    32461 agaagcgcag agctggcagc aacgccagaa cgaattaacc gcgctgcaaa accgtattca
    32521 gcagctgaca ccgattctgg aaacgttgcc gcaaagtgat gatctcccgc acagcgaaga
    32581 aactgtggcg ctggataact ggcggcaggt tcatgaacaa tgtctcgcat tacacagcca
    32641 gcagcagacg ttacagcaac aggatgttct ggcggcgcaa agtctgcaaa aagcccaagc
    32701 gcagtttgac accgcgctac aggccagcgt ctttgacgat cagcaggcgt tccttgcggc
    32761 gctaatggat gaacaaacac taacgcagct ggaacagctc aagcagaatc tggaaaacca
    32821 gcgccgtcag gcgcaaacgc tggtcactca gacagcagaa acgctggcac agcatcaaca
    32881 acaccgacct gacgggttgg ctctcactgt gacggtggag cagattcagc aagagttagc
    32941 gcaaactcac caaaagttgc gtgaaaacac cacgagtcaa ggcgagattc gccagcagct
    33001 gaagcaggat gcagataacc gtcagcaaca acaaacctta atgcagcaaa ttgctcaaat
    33061 gacgcagcag gttgaggact ggggatatct gaattcgcta ataggttcca aagagggcga
    33121 taaattccgc aagtttgccc aggggctgac gctggataat ttagtccatc tcgctaatca
    33181 gcaacttacc cggctgcacg tgcgctatct gttacagcgc aaagccagcg aggcgctgga
    33241 agtcgaggtt gttgatacct ggcaggcaga tgcggtacgc gatacccgta ccctttccgg
    33301 cggcgaaagt ttcctcgtta gtctggcgct ggcgctggcg ctttcggatc tggtcagcca
    33361 taaaacacgt attgactcgc tgttccttga tgaaggtttt ggcacgctgg atagcgaaac
    33421 gctggatacc gcccttgatg cgctggatgc cctgaacgcc agtggcaaaa ccatcggtgt
    33481 gattagccac gtagaagcga tgaaagagcg tattccggtg cagatcaaag tgaaaaagat
    33541 caacggcctg ggctacagca aactggaaag tacgtttgca gtgaaataac tattcagcag
    33601 gataatgaat acagaggggt gaattatctc ttggccttgc tggtcgttat cctgcaagct
    33661 atcactttat tggctacggt gattggtagc cgttctggtg gttgtgatgg tggtatgaaa
    33721 aaagtcattt tatctttggc tctgggcacg tttggtttgg ggatggccga atttggcatt
    33781 atgagcgtgc tcacggagct ggcgcataac gtaggaattt cgattcctgc tgccgggcat
    33841 atgatctcgt attatgcact ggtggtggtg gtcggtgcgc caatcatcgc actcttttcc
    33901 agccgctact cactcaaaca tatcttgttg tttctggtgg cgttgtgcgt cattggcaac
    33961 gccatgttca cgctctcttc gtcttacctg atgctcgcca ttggtcggct ggtatccggc
    34021 tttccgcatg gcgcattttt tggcgtcgga gcgatcgtgt tatcaaaaat tatcaaaccc
    34081 ggaaaagtca ccgccgccgt ggcggggatg gtttccggga tgacagtcgc caatttgctg
    34141 ggcattccgc tgggaacgta tttaagtcag gaatgctggc gttacacctt tttattgatc
    34201 gctgttttta atattgcggt gatggcatcg gtctattttt gggtgccaga tattcgcgac
    34261 gaggcgaaag gaaatctgcg cgaacaattt cactttttgc gcagcccggc cccgtggtta
    34321 attttcgccg ccacgatgtt tggcaacgca ggtgtgtttg cctggttcag ctacgtaaag
    34381 ccatacatga tgtttatttc cggtttttcg gaaacggcga tgacctttat tatgatgtta
    34441 gttgggctag ggatggtgct gggaaatatg ctaagtggca ggatttcagg acgttattca
    34501 ccactgcgca ttgcagcagt gactgacttt atcattgtac tggcactgct gatgctcttt
    34561 ttctgcggcg gcatgaaaac aacgtcgctt atttttgctt ttatttgttg cgcgggatta
    34621 tttgcccttt cagcacctct gcaaatattg ttactgcaaa atgccaaagg aggagagtta
    34681 ttaggtgccg caggtgggca aatagcgttt aacctcggta gcgccgtcgg cgcatattgc
    34741 ggaggtatga tgctgacgct ggggctggca tataattacg tggcgctgcc tgccgccctg
    34801 ctttcgtttg ctgcgatgtc atcgttgctg ctgtatggtc gctataagcg ccagcaagcg
    34861 gcggatagtc cggtgctggc gaaaccactg gggtagatta ctgtcacggt gcgcgttgct
    34921 tattgccgga tgcggcgtga acgccttatc cgccctacgc ggttctggca cattttgtag
    34981 gcctgataag acgcggcaag cgtcgcatca ggcatgatgc gccaattgcc tacgtttttt
    35041 actcttgtgg ccataaccac gcagcgccgc gtacgccgct ggaatcaccg tgcttcgcct
    35101 tactcaccgg cgtttcacat tcgccgccga agacaaattg tttaatcaac tgcccaaccg
    35161 tttgatataa acggtctaca ttgctcatcc cgccccccag gacaatcaca tccggatcga
    35221 gaatattcac gacatgtgcc agcgattttg ccagccgcag ctcgtagcga cgcaatgcca
    35281 gttccgctac cggatcgctt tcttcaacca ggcggataat ttcactgcct ttcagcgcat
    35341 gtccgctcaa acgacgataa tccgtcgcga atcccgtgcc cgaaataaag gtttcaatac
    35401 aaccttgttt accgcaataa caagggactt cctcgcgata acgcagttcg tcttcgtcca
    35461 tccacggtag cggattgtgt ccccactcac ctgccgtgcc attgccgccg atatgcgtcc
    35521 gcccattgaa tgccacgccc gcgccgcatc ccgtgccgat aatcacggca aataccgtct
    35581 gcgctcccgc tgccgcgcca tctactgctt ctgaaaccgc cagacagttg gcatcatttg
    35641 ccagccgcac ttcccgctgc aacctcgcgc ttaagtcttt atcgaatggc tgaccgttga
    35701 gccaggttga attgacattc ttcaccacac cggtgtaggg cgaaatcgaa cctggaatgc
    35761 ccatacctac cgttccgcgc tgccctgtcg cctgctccgc catatcaacc aacgtggcga
    35821 tcgtttcaat agtctgccgg taatcatcac gcggcgtggg cagacgatgg cggtacaact
    35881 gctcccctgc atcgcccagt gcaatcactt cagttttggt gccgcctaaa tcgataccta
    35941 tacgcactgt actctcctta tttttttcaa tatcaatagc gtagagacgg acaaccggat
    36001 tggcaatgca aggccgccga caattcgtta tcatgcccgc taaatttaac gacaaggccg
    36061 tggaaattat catgctgtgg ttcaaaaatt taatggttta ccgtcttagc cgcgagattt
    36121 cgctgcgtgc agaagagatg gaaaaacagc tagcctcgat ggcatttacc ccatgcggca
    36181 gccaggacat ggcgaagatg ggctgggttc ctccgatggg atcgcacagc gatgcgttaa
    36241 cgcacgttgc caatggtcaa attgttatct gcgcgcgcaa agaagaaaaa atcctcccgt
    36301 ctccggtgat taaacaggcg ctggaagcga aaatcgccaa actggaagcg gaacaggcgc
    36361 gtaagctgaa gaaaaccgaa aaagattcgc tgaaagacga agtgctgcac tctctgctgc
    36421 cgcgtgcttt cagccgtttt agccagacaa tgatgtggct cgacacggtt aacggtttga
    36481 ttatggtgga ctgcgccagt gccaaaaaag cggaagatac gctggcatta ctgcgtaaaa
    36541 gcctggggtc gttaccggtt gtaccattga gcatggaaaa cccgattgaa ctgacgctga
    36601 ccgaatgggt tcgctccggt agtgcggcac agggcttcca gctgcttgat gaagccgagc
    36661 tgaaatcgtt gctggaagat ggcggcgtga tccgcgcgaa gaaacaagat ctgaccagcg
    36721 aagagatcac caatcacatt gaagccggaa aagtggtgac taaactggcg ctcgactggc
    36781 agcagcgcat tcagtttgtg atgtgcgacg atggttcgct caagcgtctt aagttctgcg
    36841 acgagctgcg cgatcaaaac gaagatatcg accgtgaaga tttcgcccag cgttttgatg
    36901 ccgatttcat cctgatgact ggtgaactgg cagcgttaat tcaaaacctg attgaaggat
    36961 taggtggcga agcacaacgt taattgctga ttttccttta atgccggatg cgacgcctgc
    37021 cgcgtcttat ccggcgtacg aagccacacc aggcatataa ttattcgcta cggcgagcaa
    37081 taatttttag cgcagcaata ttatgcgttt tacgccgtaa ctgatctaat cgcctcatcc
    37141 ctattcgcct gtcagcagca cgcagaatat tcactaaccc ccatttatcg ctggtaaatt
    37201 gttttgttaa cagctccgct tttggggtat caatatattc acgcaataaa tcagaaattg
    37261 aacaaatatc cgtcaggtaa ggataccagc aagtttcacc gtgaaaatta cgcactccag
    37321 cgttacctgc aataaagtct ttcgggtaat cccagatcac gtttttatcc aacgtgatcc
    37381 aataacgcgg taaatcggta cttccaccat tttggctgcg catgggataa cgggtacagt
    37441 gaatttgcag gttgatcgtt ggcgtcagta agtcatagat ctcccgctgt aacttgctcc
    37501 atggacgttg tgtcattgtt tttcctcaag ccggtgacgc gcttcactgc gaattttccg
    37561 cgttgccccg gttttcacta aatcacgtaa ctgcgaaaga tcgggttgtt cgacaaataa
    37621 gagtaatgat ggatcctctt ttaaccacgc cgttttcaca tcggcagcca gctgtggatt
    37681 attaatagcc agcgaccgat cctgaggctc agtcaatgtc gttaacaacg acgccggggt
    37741 atgtgcgttg cgtaatgccg tttggcgcaa atgatcattg cgactttgca gcaatgcacg
    37801 ccaccattca tcatctgatt tatcttttat cgtagccagt tccgcaaaaa atacctggag
    37861 atcttcactg atatttatat caaccttgat gaattatgaa tatatcggaa attgaaaaaa
    37921 tcgcagcaaa ggaaatatta ttatgggtta aagagaggat atttcagaaa aatgccctcc
    37981 cgttcaaggg gaaggcgagg aattacagat agcggcacag ataagaggta ggttcggcaa
    38041 cttgaagatg aaactcactg tgaccgggaa cattaaacac cgaaccggct tcatacactt
    38101 gccagtcggt cgcgtcaggc agtaacacat tcagcgcgcc actgattacc gtcatctctt
    38161 ccggctcagc ggtgctgaag gtgtattcgc cttcaaccat aacacccacg ctggcgcgac
    38221 cagtgctgct gctggaaaag ccgatcgatt tcactttgcc ggaaaagtac tcattacttt
    38281 gaagcataaa ctggccctgt ctggtagttg aacatctact tcactatagg ggccagaggc
    38341 gctggctgtc acgcaaaatt acaccagtaa ttccgcagcc agccgtgcaa tcaatacgtt
    38401 agagagcaag acaggaacat cgagctgttt ttgcagtaaa tcgcgatgac gttggtgaaa
    38461 tcctaaacaa tccagcatga tgacatcagc cccttttgcc agtaattctt tcccagcatc
    38521 aatgattttt tgttctgaat catgaatggg gttacccaat gaaaataccg gcgatttctg
    38581 caaaatttgc catttttgcg cctgaacggg cagcatctcc tcaaccggaa cgataacccc
    38641 cacctgatga tcttcaacaa tagaggaaac cagtggaggc aatattcgcg acggctcaag
    38701 aaagatcgta ttacgcgcag tcatactact aatgtttgct gtactcatta ataaaatgac
    38761 gtcataaccc tgattatcga gcacttcaac cacaccttgc agatctcgct ccactttacg
    38821 acgcgaaaca tgggccagct ggttgtcatt taataatgtg agaatggtgt cttcgcctgc
    38881 ttctggcgcg tactccgcca tcacttcttc acgacttaac ttccccagca ggctatgatg
    38941 ggaaatatta tcttcgtcaa tgtattccgt caggagcggc aaaacctcct gcataggtac
    39001 aatgccgatg gtcaggatcg ccaacgacgc actcattgtt catccacctt acttttcttt
    39061 cgtcgttaat taccgggcaa gtgtgaagca ccactgctga cacttacttg tgctacaaat
    39121 gacaaaaagc gtagcagcag acgtgcacgg cataatgtaa agattccaaa tgattccagt
    39181 aatggaatgt tattgtttaa tattctaatt attagaaaaa cataaattat gaaaaatgtg
    39241 acgcagatcc cttgtagtgg cactcagaat cccttccgag cagtctgatg cggtcactct
    39301 ttgtcagggt cctcatagcg ttttttcgca tccatcgctt cctgagccgt cggatgttca
    39361 ctgatcaacg agtctggttt aggatgatcg gctctgagtt ggtaccaggt taccgtctgt
    39421 cctggctttc ctttttcaat cgtcactata tatgcttcac gaggataagg cggtttcgtt
    39481 ggcatcgttc ttccttattt cacgggatga acgttaagta taggcgctct aaaatcaaca
    39541 attgatcgtc tgtgccaggg cgctacgaat ttcagaaatc acctggctgg gttcgtttgt
    39601 tgcgtcgatg ataatatgcg caacttcgcg atatagcgca tcgcgttctt ccagcacttc
    39661 ctgaacttct tcgctcagcg gttttcccgt taaggttggc cgtaaatctt cttccggtgc
    39721 agcttgcagt cggttaacca ggactgatac tggcgcacac aaataaacca cgatcccgtt
    39781 attttgcatg aagtgacgat taaattccgt cagaataatg ccgccgcctg tagcgataac
    39841 ggtggatgcc gcagttaccg cttccagcgc cgccgtttct ctggcgcgaa atcccgccca
    39901 ctcttccctt tcgacgatct ccgcgaccgt catattgagc tgtgattgca accactgatc
    39961 ggtatcgaca aaccgacggt taagcgaatc ggcaagggcc attccgaccg ttgttttacc
    40021 acagccccga ggcccgatca gaaaaagagg ttgtgtcatc gtgggttttc cccaataggt
    40081 cgcaatgcgg cgaaagccgg tgtcatgaga atagcgatca taccatcaaa ctagtacaat
    40141 ttcgattgta aagaaaaaat tccacttaaa gtaaaaatct caatacatcc cttactatac
    40201 caataaatat tcaagaatga agtgtaaata ataaattaca tttagccacg actacgttgc
    40261 acttccagcc accacttctc cagctccgcg gcaaaggcct ggcggtcacg ttgtgaaagg
    40321 ctatctggtc cgccggtctg gatcccactg gcacgtaagg tatccataaa atcgcgcatc
    40381 gtcaggcgct cacgaatggt cgctggcgtg taacgttcgc cgcgcgaatt aagcgccgct
    40441 gcgcctttct cgatggcttc agcagccaaa ggtatatctg cggtgatcac caaatcgccc
    40501 gcttcacact gccggacaat ttcgttatcg gcaacgtcga aacctgccgc gacgcgcagt
    40561 gtacgaataa atcgcgatgg cggcacgcgt aaactctggt ttgctaccag taccagcggc
    40621 atctgcatac gttccgccgc gcgatacaaa atctctttaa ttacattggg acacgcgtcg
    40681 gcatccaccc aaattgtcat aaagtcatcc tttgttaggt aatcctctat tgtgtcgcgc
    40741 ttttgccttc cggcatagtt ctgtttatgc ttctgccagc gattatcaaa acaatgaatt
    40801 tcacggcagg agtgaggcaa tggaaaagaa aatcggtttt attggctgcg gcaatatggg
    40861 aaaagccatt ctcggcggtc tgattgccag cggtcaggtg cttccagggc aaatctgggt
    40921 atacaccccc tccccggata aagtcgccgc cctgcatgac cagttcggca tcaacgccgc
    40981 agaatcggcg caagaagtgg cgcaaatcgc cgacatcatt tttgctgccg ttaaacctgg
    41041 catcatgatt aaagtgctta gcgaaatcac ctccagcctg aataaagact ctctggtcgt
    41101 ttctattgct gcaggtgtca cgctcgacca gcttgcccgc gcgctgggcc atgaccggaa
    41161 aattatccgc gccatgccga acactcccgc gctggttaat gccgggatga cctccgtaac
    41221 gccaaacgcg ctggtaaccc cagaagatac cgctgatgtg ctgaatattt tccgctgctt
    41281 tggcgaagcg gaagtaattg ctgagccgat gatccacccg gtggtcggtg tgagcggttc
    41341 ttcgccagcc tacgtattta tgtttatcga agcgatggcc gacgccgccg tgctgggcgg
    41401 gatgccacgc gcccaggcgt ataaatttgc cgctcaggcg gtaatgggtt ccgcaaaaat
    41461 ggtgctggaa acgggagaac atccgggggc actgaaagat atggtctgct caccgggagg
    41521 caccaccatt gaagcggtac gcgtactgga agagaaaggc ttccgtgccg cagtgatcga
    41581 agcgatgacg aagtgtatgg aaaaatcaga aaaactcagc aaatcctgat gactttcgcc
    41641 ggacgtcagg ccgccacttc ggtgcggtta cgtccggctt tctttgcttt gtaaagcgcc
    41701 aaatctgccg atttcaacca ctcacgatag tgactcattt gtgggttcag cggcgcaacc
    41761 cccacactaa tccgtaaagt tacctgtggc gtattcggca agcgtaacgt gtttagccct
    41821 tcacgcaccc gtaacatggc ggtaatggcg ctctcagctg gcgtaccgga catgattact
    41881 gcaaactcat cgccgccaaa ccgaccaatc acatcgctac cgcgcagggt aatttgtaac
    41941 tgtcgggtaa gcgccacaat cgcttcatcg cccacatcat gaccccaggt atcgttgatg
    42001 ctcttgaaat ggtcgatatc gataatcagt aacgttgcat cgcgattatg ccgccgacag
    42061 ttatcaaatt cattgcgtaa catagtttcc caatgacgtc ggttatacac gcccgtcatg
    42121 ccgtcgcgag tactcatgac ctgcaacctg cgtttatgtt ccgccagttt ggttgccgtc
    42181 tggtagctga cccagccaaa catcagagga taaacgacaa taatgggaag ggagagccac
    42241 cattccagcg gagcactatt gaacgacacg gtaatgcccg taagctcgag ggtgacaagg
    42301 caggaaacca ccatcaacac cagacccgcg acaaacagac gggggccgcc tgcccccatc
    42361 aaattcagac acagaatcat caacatagcg gtggaaggca gcacgtttac gcccattacg
    42421 cctacccaca ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc ccggctaagc
    42481 ggatcgacgg ccctgctcgc tatctgccag gctaaatgcg gccagacgaa cgcccagccg
    42541 accaacacca gccaccacca gcccggcggc gggtgcgaaa ccagcgttga agcaatcggt
    42601 aagaacatgc cagccaggcc aaccgcacgg ggtagtctga cgcgacgggc gaagcgcagc
    42661 ccggaccgct gatgttcgtt ttgaggagtt aaaggaggtt cctccccatg cgccgccgct
    42721 tttttgaaaa agttttcatc attcattatt tttgggaaca ttcagaaaca attttcccaa
    42781 attatagaga cggggctaaa ggcagaagta tgaaatttcg ggtgagccac tttcgtagct
    42841 cacccgttag cact
//
LOCUS       DADLTY010000010        42790 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.010, whole genome shotgun sequence.
ACCESSION   DADLTY010000010 DADLTY010000000
VERSION     DADLTY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.010"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(88..624)
                     /gene="ssb1"
                     /locus_tag="JN334_000471"
     CDS             complement(88..624)
                     /gene="ssb1"
                     /locus_tag="JN334_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HAY7436734.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            878..3700
                     /gene="uvrA"
                     /locus_tag="JN334_000472"
     CDS             878..3700
                     /gene="uvrA"
                     /locus_tag="JN334_000472"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAY7436735.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3735..4091)
                     /locus_tag="JN334_000473"
     CDS             complement(3735..4091)
                     /locus_tag="JN334_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY7436736.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4095..4511)
                     /locus_tag="JN334_000474"
     CDS             complement(4095..4511)
                     /locus_tag="JN334_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAY7436737.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4622..5335)
                     /locus_tag="JN334_000475"
     CDS             complement(4622..5335)
                     /locus_tag="JN334_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B acid phosphatase"
                     /protein_id="HAY7436738.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5737..5941
                     /locus_tag="JN334_000476"
                     /pseudo
     CDS             5737..5941
                     /locus_tag="JN334_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6444..7637)
                     /gene="tyrB"
                     /locus_tag="JN334_000477"
     CDS             complement(6444..7637)
                     /gene="tyrB"
                     /locus_tag="JN334_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HAY7436739.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7890..8969)
                     /gene="alr"
                     /locus_tag="JN334_000478"
     CDS             complement(7890..8969)
                     /gene="alr"
                     /locus_tag="JN334_000478"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAY7436740.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9022..10437)
                     /locus_tag="JN334_000479"
     CDS             complement(9022..10437)
                     /locus_tag="JN334_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HAY7436741.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10520..11503
                     /locus_tag="JN334_000480"
     CDS             10520..11503
                     /locus_tag="JN334_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HAY7436742.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11669..11911)
                     /gene="pspG"
                     /locus_tag="JN334_000481"
     CDS             complement(11669..11911)
                     /gene="pspG"
                     /locus_tag="JN334_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HAY7436743.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12045..13082)
                     /gene="dusA"
                     /locus_tag="JN334_000482"
     CDS             complement(12045..13082)
                     /gene="dusA"
                     /locus_tag="JN334_000482"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAY7436744.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13445..14152)
                     /locus_tag="JN334_000483"
     CDS             complement(13445..14152)
                     /locus_tag="JN334_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HAY7436745.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LTDGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(14176..14430)
                     /locus_tag="JN334_000484"
     CDS             complement(14176..14430)
                     /locus_tag="JN334_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436746.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            14748..15263
                     /gene="zur"
                     /locus_tag="JN334_000485"
     CDS             14748..15263
                     /gene="zur"
                     /locus_tag="JN334_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HAY7436747.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15305..15514)
                     /locus_tag="JN334_000486"
     CDS             complement(15305..15514)
                     /locus_tag="JN334_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAY7436748.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15630..17009)
                     /gene="dinF"
                     /locus_tag="JN334_000487"
     CDS             complement(15630..17009)
                     /gene="dinF"
                     /locus_tag="JN334_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HAY7436749.1"
                     /translation="MPLGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHW
                     RNGTWFAAT"
     gene            complement(17028..17636)
                     /gene="lexA"
                     /locus_tag="JN334_000488"
     CDS             complement(17028..17636)
                     /gene="lexA"
                     /locus_tag="JN334_000488"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAY7436750.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17746..18114)
                     /locus_tag="JN334_000489"
     CDS             complement(17746..18114)
                     /locus_tag="JN334_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAY7436751.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18285..20708
                     /gene="plsB"
                     /locus_tag="JN334_000490"
     CDS             18285..20708
                     /gene="plsB"
                     /locus_tag="JN334_000490"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HAY7436752.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20863..21735)
                     /gene="ubiA"
                     /locus_tag="JN334_000491"
     CDS             complement(20863..21735)
                     /gene="ubiA"
                     /locus_tag="JN334_000491"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAY7436753.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21748..22245)
                     /gene="ubiC"
                     /locus_tag="JN334_000492"
     CDS             complement(21748..22245)
                     /gene="ubiC"
                     /locus_tag="JN334_000492"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAY7436754.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22468..24048)
                     /locus_tag="JN334_000493"
                     /pseudo
     CDS             complement(22468..24048)
                     /locus_tag="JN334_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24277..25197)
                     /gene="malM"
                     /locus_tag="JN334_000494"
     CDS             complement(24277..25197)
                     /gene="malM"
                     /locus_tag="JN334_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HAY7436755.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25349..26689)
                     /gene="lamB"
                     /locus_tag="JN334_000495"
     CDS             complement(25349..26689)
                     /gene="lamB"
                     /locus_tag="JN334_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HAY7436756.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26761..27876)
                     /gene="malK"
                     /locus_tag="JN334_000496"
     CDS             complement(26761..27876)
                     /gene="malK"
                     /locus_tag="JN334_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HAY7436757.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28241..29431
                     /gene="malE"
                     /locus_tag="JN334_000497"
     CDS             28241..29431
                     /gene="malE"
                     /locus_tag="JN334_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HAY7436758.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29585..31130
                     /gene="malF"
                     /locus_tag="JN334_000498"
                     /pseudo
     CDS             29585..31130
                     /gene="malF"
                     /locus_tag="JN334_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="JN334_000499"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="JN334_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HAY7436759.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32406..33881
                     /gene="xylE"
                     /locus_tag="JN334_000500"
     CDS             32406..33881
                     /gene="xylE"
                     /locus_tag="JN334_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HAY7436760.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="JN334_000501"
     CDS             complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="JN334_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HAY7436761.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34461..34604)
                     /locus_tag="JN334_000502"
     CDS             complement(34461..34604)
                     /locus_tag="JN334_000502"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436762.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34605..34883
                     /locus_tag="JN334_000503"
     CDS             34605..34883
                     /locus_tag="JN334_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436763.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34930..37026)
                     /locus_tag="JN334_000504"
     CDS             complement(34930..37026)
                     /locus_tag="JN334_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAY7436764.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37026..37763)
                     /locus_tag="JN334_000505"
     CDS             complement(37026..37763)
                     /locus_tag="JN334_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY7436765.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37760..38398)
                     /locus_tag="JN334_000506"
     CDS             complement(37760..38398)
                     /locus_tag="JN334_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY7436766.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38512..38754)
                     /locus_tag="JN334_000507"
     CDS             complement(38512..38754)
                     /locus_tag="JN334_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436767.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="JN334_000508"
     CDS             complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="JN334_000508"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAY7436768.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41391..42740
                     /gene="lysC"
                     /locus_tag="JN334_000509"
     CDS             41391..42740
                     /gene="lysC"
                     /locus_tag="JN334_000509"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HAY7436769.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT        10642 a        11013 c        10504 g        10631 t
ORIGIN      
        1 ctccataacg gaggcttctt ttttatggct ggcagatgct ttaatcatcc accttaaaac
       61 aatataacct attgttttaa tgacaaatca gaacggaatg tcatcatcaa agtccatcgg
      121 cggctcgtta gacggcgctg ccggagcgga ctgctgcggg cgagactgcg cgccgccgct
      181 gaactgattg ccaccctgcg gctgctgagg ctgaccccaa ccgccctgcg gctgaccacc
      241 accgatattg ccacctgccg gagcgccacc accctgacga ccacccagca tctgcatggt
      301 gccgccaacg ttcaccacga cttctgtggt gtagcgatcc tgaccggatt gatcggtcca
      361 tttacgggta cgcagctgac cttcgatata aacctgagaa cctttacgca gatattcgct
      421 cgccacttct gccagtttgc cgaacagcac aacgcggtgc cattcagtct gttctttcat
      481 ctcgccggtc gctttatcac gccaggattc ggaagtagcc agcgtaatgt tggcaactgc
      541 gccaccattt ggcatgtagc gtacttccgg gtcctgaccc agattaccaa cgagaataac
      601 cttgtttacg cctctgctgg ccatgttcgt gtctcctgaa aaaaatcgtt ctgaataagt
      661 gtaaacgcgc gattgtacca ttaccaatag cgcttttact atgttgtgac ctcggttccg
      721 ggaaacaaac ctggccagac attgttacac aacactccgg ataatgcatt ccaatactgt
      781 atattcattc aggtcaattt gtgtcataat taaccgtttg tgatcgccgg tagcaccatg
      841 ccaccgggca aaaaagcgtt taatccggga aaggtgaatg gataagatcg aagttcgggg
      901 cgcccgcacc cataatctca aaaacatcaa cctcgttatc ccccgcgaca agctcattgt
      961 cgtgaccggg ctttcgggtt ctggcaaatc ctcgctcgct ttcgacacct tatatgccga
     1021 agggcagcgc cgttacgttg aatccctttc cgcctacgcg cggcagtttc tgtcactgat
     1081 ggaaaagccg gacgtcgatc atattgaggg gctttctcct gccatctcaa ttgagcagaa
     1141 atcgacgtct cataacccgc gttctacggt ggggacaatc accgaaatcc acgactattt
     1201 gcgtttgttg ttcgcccgcg tcggcgaacc gcgctgtccg gaccacgacg tcccgctggc
     1261 ggcgcaaacc gtcagccaga tggtggataa cgtgctgtcg cagccggaag gcaagcgtct
     1321 gatgctgctc gcgccaatca ttaaagagcg caaaggcgaa cacaccaaaa cgctggagaa
     1381 cctggcaagc cagggttaca tccgtgctcg tattgatggc gaagtctgcg atctttccga
     1441 tccgccgaaa ctggaactgc aaaagaaaca taccattgaa gtggtggttg atcgcttcaa
     1501 ggtgcgtgac gatcttaccc aacgtcttgc ggagtcgttt gaaaccgcgc tggaactttc
     1561 cggtggtaca gcggtagtgg cggatatgga cgacccgaaa gcggaagagc tgctgttctc
     1621 cgccaacttc gcctgcccaa tttgcggcta cagtatgcgt gaactggagc cgcgactgtt
     1681 ttcgtttaac aacccggcag gtgcctgccc gacctgtgac ggccttggcg tacagcaata
     1741 tttcgatcct gaccgcgtga tccaaaaccc cgagctgtca ctggctggcg gtgcgatccg
     1801 tggctgggat cgccgcaact tctattactt ccagatgctg aaatcgctgg cagatcacta
     1861 taagttcgac gtcgaagcgc cgtggggcag cctgagcgcg aacgtgcata aagtggtgtt
     1921 gtacggttct ggcaaagaaa acattgaatt caaatacatg aacgatcgtg gcgatacctc
     1981 catccgtcgt catccgttcg aaggcgtgct gcacaatatg gagcgccgtt ataaagagac
     2041 agaatccagt gcggtacgtg aagaattagc caagtttatc agcaatcgcc catgcgccag
     2101 ctgcgaaggg acgcgtctgc gtcgggaagc gcgccacgtt tatgtcgaga atacgccgct
     2161 gcctgctatc tccgacatga gcatcggtca tgcgatggaa ttcttcaaca atctcaaact
     2221 cgctggtcag cgggcgaaga ttgcggaaaa aattcttaaa gagatcggcg atcgcctgaa
     2281 attcctcgta aacgtcggcc tgaattacct gacactttcc cgctcggcag aaacactttc
     2341 cggcggtgaa gcccagcgta tccgtctggc gagccagatt ggtgcgggcc tggttggcgt
     2401 tatgtacgtg ctggacgagc cgtctatcgg cctgcaccag cgcgataacg agcgcctgtt
     2461 gggtacgctt atccatctgc gcgatctcgg taataccgtg attgtggtgg agcatgacga
     2521 agacgcaatt cgcgccgctg accatgtgat cgatatcggt cctggtgcgg gtgttcacgg
     2581 cggtgaagtg gtcgcagaag gtccgctgga agcgattatg gcggtgcctg aatcgttgac
     2641 cgggcagtac atgagcggta aacgcaagat tgaagtgccg aagaaacgcg ttccggcgaa
     2701 tccagaaaaa gtgctgaagc tgacaggcgc acgcggcaac aacctgaagg acgtgacgct
     2761 cacgctgcca gtcggtctgt ttacctgcat cacaggggtt tcaggttccg gtaaatcgac
     2821 gctgattaac gacacactgt tcccgattgc ccaacgccag ttgaatggtg cgaccatcgc
     2881 cgaaccggca ccgtatcgcg atattcaggg actggagcat ttcgataaag tgatcgatat
     2941 cgaccaaagc ccaattggtc gtactccacg ttctaacccg gcgacctata ccggcgtgtt
     3001 tacgcctgtg cgcgaactgt ttgcgggcgt accggaatcc cgtgcgcgtg gttatacgcc
     3061 aggacgtttc agctttaacg tccgtggcgg gcgctgcgaa gcctgtcagg gcgacggtgt
     3121 gatcaaagtg gagatgcact tcctgccgga tatctacgtg ccgtgcgatc agtgcaaagg
     3181 taaacgctat aaccgtgaaa cgctggaaat taagtacaaa ggcaaaacca tccacgaagt
     3241 gctggatatg accatcgaag aggcgcgtga gttctttgat gcggtgccag ctctggcgcg
     3301 taagctgcaa acgttgatgg acgttggtct gacgtacatt cgcctggggc agtccgcaac
     3361 cacactttct ggtggtgaag cccagcgcgt gaagctggcg cgtgagctgt caaaacgcgg
     3421 caccgggcag acgctgtata ttctcgacga gccgaccacc ggtctgcact tcgccgatat
     3481 tcagcaactc ctcgacgtgc tgcataaact gcgcgatcag ggcaatacca ttgtggtgat
     3541 tgagcacaat ctcgacgtga tcaaaaccgc tgactggatt gtcgacctgg gaccagaagg
     3601 cggcagtggc ggcggcgaaa tcctcgtctc cggtacgcca gaaaccgtcg cggagtgcga
     3661 agcttcgcat acggcacgct tcctcaagcc gatgctgtaa tcgttaaggc cgctttctga
     3721 gcggcctttt cctttcagag ttgtaccagc aatttacgct tttcttccgg tagtaaattc
     3781 actgcctgct gataagacgc atccaccaga taatagattt gcgaatccgg cagcgaaccg
     3841 tcgagataca cggtgctcca gtgcgcttta ttcagatggc ggctaggacg cacatcgctg
     3901 tgctgctgac gtagcagctc cgccagctcc gggctggttt tcagcgaaac agctgggcga
     3961 ttttctactt ctttcaccat cgcaaacagt acatcttcca ctttgatctg cgtcgctttc
     4021 cagtcgttat gcacgctctg ttctgcgctt ggttttgcca tgcaatattg tagcaactcc
     4081 gaaatggtca ttttttactc cccttgtagt gtcgcgatga tgcgacgcga tccgccgtgg
     4141 atgcgatgtt cccccagcca gatgccttgc caagtgccgg tctgaatacg ccctttatgt
     4201 accggcaata caagcgatgt tcccagcatt gaggatttga tatgagaagg catatcgtct
     4261 gctccctcat agtcatgctc ataatttccg ttgtcgggaa cggtgcggag gaaaaaacac
     4321 tccatgtcgt ggcgtacggt gggatcgcag ttctcattaa gtgtcagaga ggcggaggta
     4381 tgttgcagca acagatgcag taagccgatg ttaacgcgcg gcatatcagc cagctgattc
     4441 agaatttcat ccgttaccag atgaaaccca cgagatttgg cgctaagcgt gagcgtcttt
     4501 tgataccaca tgtgctgctc cttgataaaa ctctcttaat cagtttgcag caagacagcg
     4561 aaaggataaa ggtgtgatta aaaaaacagc attcgggaga gcatcactct ctcccgctct
     4621 gtcagtattc tgaattgacg atcacctctt caccaaacgc acccgcttgt ggcaagggtt
     4681 tgtaggtaga gttggaggcg cgcagaatgc ggataccacg agcgccgaca tcgcgtgcgg
     4741 cggtaatatc gttatcggaa tcgccataaa aaattcggat atttttatcc tgcagccatt
     4801 gcgattttgt attttgccct ggtttatcgc ccgcaaagat taccggattc atgttggtgg
     4861 caggaatatg aaaattatcc gccagcgttt ttgaaaccgt ttctgttttc gtcgggctac
     4921 gaccagtcac aaagaagatc gcgtcaccgc ggcgtacatg catatcaatc agctggcgag
     4981 cgacctcttt tggaatgctg aattcatccc agccattatt cattttttcc cagaacacag
     5041 gatttttcag ataatcttcg ctttctggcg agaaggtttt tttgccgcgc cagaaccccg
     5101 gactggaaaa aagtaccgtg tcatcgatat caaaccccac cgccattggc ggacgccctg
     5161 cgaggctatt ttcaatttgt gcgaccgaaa cccaatgaat tggtgcctgt tcagcaagcc
     5221 tggcaacgtt agtcccaggg ttaagcggtg aaggagatga ggccagggca acagcggaac
     5281 tgtttagagc gaacaataag caaacggcac tgattgcctg tgtgatcttg cgcatatttt
     5341 tccctaaata gtcagtatgt tgaaactttt tattgtgaga tttgttgcaa aaaccatctg
     5401 accataacgc cagctgccgg ggaaaggaag gattatctgc ggtttttgtg atgaaaagca
     5461 ggatgattat atttaaccca gtaaatacgt tcctgtcttt ttttgtataa aaaatgttta
     5521 ttactcgatg taaggataca ttaattttac gtttattatg atgaatcctc ggcaaatgag
     5581 cgaaatttga tagaacattt atctgctgta cgatttaatg aacttaatga tacagcatta
     5641 caaaaacaat cgaagtttat aaagatgatt tctgattgac cagcccccta ccctacaatg
     5701 gtgattaatt ttattactcg cgatggagat gatgatatga atatatcata tgtgaatagc
     5761 aacaaaacga catctttacc tgttgagcta gatgcactaa ataataaaga tatctcttat
     5821 gccaaagatt tctttttata catagaaaca cagttaaaaa ttgcaaaaga tttttgtaga
     5881 cctggagaag aagtatcaag ttctattgca agtaaagttt ttcacgcatt tattgattta
     5941 gttaacaaaa tcaggggtaa gaaagacttt atgtatattt gcacgctttg ctgttttgcc
     6001 gaggaggtta aaggtgatta ttctcattac aggacctttt tatttgatat tggtaatcaa
     6061 tataaggtta aacttacaca aagcggaaaa aagagttctc tttaacatta gaatttaacg
     6121 atactataat tgaatctcag aaagtcacag gcaataaggc aaagcatatt cttgaggata
     6181 tagaaatatt ctatcgtaac aaacccgata cttattatta aatttaataa tggttagatg
     6241 tgcagaaatt attactccac acgaaaaaat agggaaagga attccagata attgggataa
     6301 acttaacaat aaaagaactg acaacgtttt attatctctc acgtagaaca acggcatcaa
     6361 aaaaatcatc tacaaagaaa tagtgaaagc gacgacagtt taatttcact gcaggctggg
     6421 taactccagc ctgctttcct gcattacatc accgcagcaa acgcctttgc cacacgttgt
     6481 acatttgccg tatttaaccc ggcgacacac atgcgaccgc tggcgatgag atagacacca
     6541 aattcttcac gtagtcggtc aacctgagcg gcacttaaac cggtataact gaacatgccg
     6601 cgctgattaa gcagataatc gaaattgcgt tctggcatct ctgtgcttaa taccttcacc
     6661 aattcctgac gcattgccag aatgcgagta cgcatctctt ctacttccgc cagccagctg
     6721 gctttcaatg cctcgtcatt cagcactgca gccaccacct gcgcaccaaa attcggcggg
     6781 ctggagtagt tgcggcgaac tgttgctttc aattgcccca gtacgcggcc agcggcttcg
     6841 gcatcttcac acagaacaga aagtccgccg acgcgctcgc cgtaaaggga gaaaattttc
     6901 gagaacgaat tgctcaccag agcgggtaat ccagcactgg caatggcgcg aatagcgtag
     6961 gcatcctctt ccataccggc actaaatcct tgataggcaa tatcgaggaa tgggataagc
     7021 tcgcgggctt tgagaatttc aatcaccgca tcccactgat cattagtgag atcggcaccc
     7081 gttgggttgt ggcaacatgg atgcagcaac acaatactgc gggcaggtaa tgttttcagc
     7141 gtcgccaaca ggtcattaaa gcgcacgctg ttagtcactt cgtcatacca ggggtaagta
     7201 ctcacttcga atccagcccc ggcgaatatt gctacgtggt tttcccaagt aggatcgctg
     7261 acccagacgc ctgattccgg gaagtagcgt ttcaggaaat ccgcgcccac tttcaatgcc
     7321 cctgagccgc caagggtttg aatggttgct acgcgctgtt gttgcagtac cggatggtcc
     7381 gcaccaaaca gcagcggcgc aatggcatgg cgatagctgt taagcccttc catcggtaaa
     7441 taaagcgaag cgccatgggg ctgcgcattc aggcgcgctt ccgcctccgc cacggctttc
     7501 aattgtggaa taattccgtc ttcgttgtag tacagaccga tacttaaatt cactttgtcg
     7561 ctgcgagggt cttctttaaa acgctccata agcgtaagaa tcgggtcgcc agcgtaggcg
     7621 tcaacttttt gaaacacgcg atggttctcc aggtttacgg gcaggtggtt aaaacacaat
     7681 aaaccggaag aaggcgaaga tcgagtggat gttcaggagc gaacggcaat tagcaacaga
     7741 gtgagactaa tgataaacgt acatccgcca gaggcacgac ctttataaga gcatgaaaga
     7801 acatcaactt attgaatttt taggatttta ttggccggat aaggcattca cgccgcatcc
     7861 ggcacagaca atcaaatatt acagaacgat taatccacgt atttcatcgc gacccttgaa
     7921 gtcaggcgcg taataagttc gtaagcgctt actttcgtca tttcagcgat acgttctacg
     7981 ggcaaacctt cgccccataa aatgaccgga tccccggctt tgtcctgcgc ctgtggacct
     8041 aagtctacgc agatcatatc catcgccacg cgcccgacaa tcggtacttc gcgaccgttc
     8101 accagcactg gcgtaccgga cggcgcggcg cgcggataac catcgccata gcccatcgcg
     8161 actacgccaa gacgggtatc acgttcgctt acccaggttc caccataacc aacaggctct
     8221 ccggctttat gctcacgcac ggcaatcagg ctggaggtta gtgacatcac tggctgacag
     8281 ccaaaatcgg caccggtgga gcgatcttcc agcggcgaga cgccataaag aatgatgccc
     8341 gggcgcaccc agtcaaaatg cgactgtggc cacagcagaa tgccacccga cgcggcaatg
     8401 gaacgttgac caggtttgcc ttcgcaaaag gtattaaaga tagcgagttg tttctcggtt
     8461 gcgccacatt ttggttcatc cgcgcgcgca aaatggctga cgatattcac cggctgacga
     8521 acgtttttgc actgggtcag gcgatgataa aacgcctcag cctgttccgg ccttacgccc
     8581 agacggtgca taccggtatc gagtttcatc cagacggtaa ccggctcgtc caagctagcc
     8641 tcttccagcg cagccagctg ttcttcgtta tgcacggcgg tatgaaaatg ttgcgcagaa
     8701 atcgtcggca gatctctggc atcaaaaaag ccttcgagta acagtacagg tttggtgatt
     8761 ccccccgcac gcagtcgcag agcttcttcg agacgggcta cgccaaaggc gtcagcatcg
     8821 gggagcgttc gcgcggtctc aagaagaccg tgaccataag cgttcgcttt caccaccgca
     8881 accattttac tggcaggtgc cagttcacga agacgttgca ggttgtgtcg cagagcgcgg
     8941 cggttaatca caacagttgc cgcttgcatt tgtgttcctt gataattgtt tgctttaatt
     9001 acctaattca taaaataatt attattcgtc gtcgtactgc ggccccgcat agttgtcgaa
     9061 gcgcgaccat tgaccgttaa aggtcaggcg taccgtcccg attgggccgt tacgttgttt
     9121 accgataata atttccgcga tgccttttaa atcactgttt tcgtgataca cctcatcacg
     9181 atagataaac atgatcaagt ccgcatcctg ctcgatagag ccagattcac gcaggtcgga
     9241 gttgaccgga cgtttgtcgg cacgttgttc cagagaacgg ttcaactggg acagcgccac
     9301 caccggcacg ttcagttctt tcgccagtgc tttcagcgag cgggagattt ctgcaatttc
     9361 cagcgtacgg ttatcggaaa gcgccggtac gcgcatcagt tgcaggtagt cgatcatgat
     9421 aagcccgatg ccgccgtgtt cacgggcaat acggcgtgcg cgggaacgca cttccgttgg
     9481 cgtcaggccg gaagagtcat cgatatagat attgcgtttt tcgagcaaaa tacccatggt
     9541 gccggaaatg cgcgcccagt cttcatcatc gagctgcccg gtacggattt tagtctggtc
     9601 aacgcgcgac agcgacgcca gagaacgcat catgatctgt tctgatggca tctccagcga
     9661 gaagataagt actggtttat cctgcaacat tgccgcgttt tcgacgaggt tcatcgcaaa
     9721 tgttgtttta cccatcgacg gacgcgcggc gacgatgatc aaatccgacg gctgcaagcc
     9781 agcggttttt ttgttgagat cgtcataacc ggtgtttacc ccggtaacgc catcgtgtgg
     9841 ctgctgaaac aactgctcaa tacgcgccac ggttgcgtcg agcacatcgg cgatgttctt
     9901 cggcccttcg tctttgtttg cacgactttc ggcaatttta aagacgcggg attcagcaag
     9961 gtccagcaga tcttcgctgg tacgcccctg cggatcaaaa ccggcttcag caatctcatt
    10021 cgcaaccgag atcatctcac ggacaacggc acgttcacgc acgatgtccg cataagcact
    10081 gatgttcgcc gcacttggcg tattttttga cagctctgcc agataagcaa aaccaccgac
    10141 gctatcgagt tgcccctggc gttccaacga ttccgcaagg gtaatcagat cgataggact
    10201 accgctttcc tgcaaacgcg ccatttcagt aaagatatga cggtgtgggc gggtgtaaaa
    10261 atcgtctgct accacacgct cggctacatc atcccagcgt tcgttatcca gcattaaacc
    10321 gcccaacacc gactgctccg cttcgatcga gtgcggaggc actttcagcc cggcaacttg
    10381 tggatcgcgt tcgcggggtt cagcctgctg tttgttgaag ggtttatttc ctgccatagt
    10441 gaatggagtt accgaaataa agaatgggtc gaaactttac catatgaagc agaccctgac
    10501 gatacgttct ggaggacaca tggcaacacg aattgaattt cacaagcacg gtggcccgga
    10561 agtacttcaa gccgtagagt tcactcctgc cgatccggcg gagaatgaaa tccaggtcga
    10621 aaataaagcc atcggcatca attttatcga cacgtatatc cgcagcggcc tttacccgcc
    10681 gccatcgcta cccagcggat taggtaccga agcggcaggc atcgtgagta aagtcggcag
    10741 tggtgtaaag catattaagg ctggcgatca tgtagtctat gcgcagtcgg cgttaggcgc
    10801 ttacagctct gtgcataaca ttaatgcgga taaagcggcg attctgcctg cggcaatttc
    10861 ttttgagcaa gctgcggcat ccttcctgaa aggcttaacg gtttattatc tgctgcgcaa
    10921 aacctatgaa attaaacccg atgaacagtt cctgttccac gcagcggctg gcggcattgg
    10981 cttaattgcc tgccagtggg caaaagccct gggcgcgaaa ctcatcggca ccgtaggaac
    11041 cgcgcaaaaa gcgcagagcg cgctaaaagc cggcgcgtgg caggttatta actatcgtga
    11101 agagaatctg gtcgagcgat tgaaagagat caccggcggt aagaaagtgc gcgtagtgta
    11161 cgattccgtg ggcagagaca cctgggaacg gtcgctggat tgcctgcaac gccgcggctt
    11221 aatggtcagt tttggcaact catcaggtgc ggttaccggt gtgaacttag gcattctcaa
    11281 tcaaaaaggc tcgttgtatg tgacacgccc ttccctgcaa ggctatatca ccacgcggga
    11341 ggaattaacc gaggccagta atgaactgtt ctctttgatt gccagcggtg tgattaaggt
    11401 cgatgtcgcc gagcagcaga aatatccgct gaaggatgcg cagcgtgcgc atgagattct
    11461 ggaaagccgg gcgacgcaag gttccagcct gctgattcca taaaagaaat agggcttcca
    11521 cctgggaagc cctttctttt tatagttcgg ctgtatgtag ggtacagcac gatgaatctg
    11581 ttagaggcgc aatagtgaca gatttgatta tcaattccta ttttgttcta aggataaaac
    11641 cttaggttgt gatcatccgc acaatccctt agtaacgcca gcggtcataa cgctgatatt
    11701 tcggcatttt tggtgcttta atcgccttaa taacccacac caccgcaatc gccagtagta
    11761 accacggcag caacttaatc atcaatgcca gcataccgcc gaggaacata atggccgtcg
    11821 ccacaaccag cgcggcgata atgcccagca acgaaacgcc ggtgaccatc agcatgacaa
    11881 aaaagccaat cacaaaaagt agttccagca tgatgctctc ccaaatatga aatctcttgc
    11941 tggcattaca agaatcatgc caaaaataat ctattgattt aacagcaaaa cgccccgcga
    12001 cggtgcgcag ggcgtggtga atttgactac tttttggtga aaagttaacg cttatccgcc
    12061 accagtttga gcgcgtgttc cagcacgtta atgtcagcac ccgctttatg ggcattttca
    12121 cttaaataac gccgccactg ccgcgcgcca ggaataccct ggaacaaacc cagcatatgc
    12181 cggataatat ggccgagata cgtcccctgg ctgagttcat gctcaatgta cggatacatg
    12241 gcacgcacta ctgccaccgg atcggcatcg gtatccgagg aaccaaagat ctcccggtct
    12301 accgccgcca gaatacccgg attctgatac gcctcgcgcc cgaccatcac gccatccata
    12361 tgttgcaaat gcgctttagc ttcttccagc gacttgatac caccgttaat cgacattgtc
    12421 agatgcggga agtcacgctt cagttgatac acacgcggat aatcgagcgg cgggatttca
    12481 cggttttctt tcgggcttaa ccccgaaagc caggctttac gtgcgtggat gatgaacatc
    12541 tcacactcgc ctttgccgga aacggtattg atgaaatcgc agagaaattc atagctgtcc
    12601 tggtcgtcga tgccaatacg cgttttcacc gtcaccggaa tcgacaccac atcgcgcatc
    12661 gctttcacgc agtcggcaac cagctgcgca ttacccatca gacacgcacc aaacatgccg
    12721 ttctgcaccc ggtcagacgg gcagccgaca ttcaggttga tctcatcata tccgcgcgct
    12781 tctgccagct ttgcacactg cgccagcgcc gccggatcgc taccgcccag ttgcaacgct
    12841 accggatgtt cttcttcact gtacgccaga taatcacctt taccgtgaat tatcgcccct
    12901 gtggtcacca tttcggtata aagcaacgta ttgcgggaaa gcagacgcaa gaaatagcgg
    12961 caatgtctgt ccgtccagtc gagcattggt gcaacactaa accgaccact ccagtgaacg
    13021 tcagtttttt caggcattgc gctggtttgg ttgatttttt gcatttcaga attaccgtgc
    13081 attttcaaat gtagagatta ttttatcgat atatcatggg gttatgttat tcagcttcac
    13141 tgttcaggag gctcaatagc ggggtactat accataacaa caggaagcgc ctgtctcatt
    13201 gcaaaagaaa attgagatcc tctcaaggca tgaagctctc acgaagtgat ggaaataatc
    13261 ttattagccg ttaactttgt taaggccaat gataaacaat ccaggttcga cgataaataa
    13321 aaaatcacac attaaactct ggtgatatat ctccctgcta atagcattga tagagaaaaa
    13381 agaacccaat aaatattggg tccttttata taatgccttc catactatcg aagaacttca
    13441 catattattt ctccgattta accccgaaca agtcataaat taatttagaa gtgtctgtaa
    13501 gtattttaat ctcttccttt gaggttgggt caaacgactt cgcgaagcta attaattgtg
    13561 gtgcagcatc tcgcattttg cttaaaatat caggatcgag cgttccttca ttcaccagat
    13621 gtgacatctt atccagatag ccatcaaaat tcatttctcc cccatctgtc agacgcttca
    13681 tctctcccag gtacttcttc atatcacgtt gatataattt ttcaaactga gctttcaggt
    13741 agctctcatt attttcatta atatacactg tttctgataa atctgcgtag gcactagaat
    13801 atgaaagtga aattaacagc gaagcaaata attttaagct gttgtcatca tgttcctggc
    13861 aggcattaac aaacgttaaa aaaccagaac ctattgcttg aaaatgtata ttcgctaaag
    13921 gttcattatc cttagattcc tcataaaata gctgaaccgt ggagggaagg ccattcttag
    13981 tttctacatc aaaagtacat tgttcaatgg gcattgcatc tttattatcc ccaggattac
    14041 atatactatt aatcgcgaaa tgaaaaatag cccggtcaac aactgatgca ataaagtcac
    14101 gatcgtttaa atcctcatca gtgcaatcgt cagtatactt attgacccac attttgagat
    14161 catttatgtg ctcaattaaa agcacatttt tttacgtgca tgccataaat tatgcgtgat
    14221 tcgttgaata aacaaatcta tttttgcaat aattatatcc cgatgacgca gcgctgcata
    14281 atcaatagat ttaccatata tattatttaa tttacctttg attgaacctc tattatccaa
    14341 tttatctttg gttgcatctt tattttcgaa ttgatttgaa ttgagtgttt tattaaagta
    14401 tccagttgca ccgggtatta tcttcaacat gattcatcta ccttttttgt cgtaccgtgt
    14461 cctccttata attcttttgc catctggtca ctatcaaaaa tcgctcataa tttctgaact
    14521 acaggtatgc gtttaaaccc agttaaataa atagcgagtt aacttgaagc aatttattta
    14581 aaaatgaatt attaattatt atcgtgttga taatggtacg ataaggcaac acaacacctt
    14641 acctacaaaa cagaaaatca acattacggc aacaataagg gttctcggtg tttttcctgg
    14701 aacatggtaa agtaaggaca ttcttaaccc ccactttgag gtgcccgatg gaaaagacca
    14761 caacgcagga gttattagcg caggctgaaa aaatctgcgc gcagcgtaat gtgcgcctga
    14821 ccccacagcg cctggaagtg ttgcgcctga tgagtctgca ggatggcgct atcagcgctt
    14881 atgatctgct tgatttactg cgcgaagctg aaccgcaagc caagccgcca acggtttatc
    14941 gcgcgctgga ttttctgctt gagcaaggtt ttgtgcataa ggtggaatcc accaacagtt
    15001 atgtgctctg tcatctgttc gaccagccca cgcatacgtc agccatgttt atttgcgatc
    15061 gctgcggcgc ggtgaaagaa gagtgtgcag aaggcgtgga agacattatg catacgctgg
    15121 cggcaaaaat ggggtttgcc ctgcggcata atgtgattga agcacatggg ctctgtgcgg
    15181 catgcgtgga agtggaagcg tgtcgtcatc ctgaacagtg ccagcatgat cactctgtgc
    15241 aggtgaaaaa gaaaccgcgt taagagggcg tacatccttg tacacgtcgg gcaggaggga
    15301 ttaattacca gcgatattca ttgcgggttt cccaatccac gacctctttt tctgcctgat
    15361 ctttctgata accataacgt tcctggattt taccgaccag ttgatcacgt ttaccttcaa
    15421 tgatcgtcat atcatcatcg gtcagtttgc cccattgctc tttcacttta cctttaaact
    15481 gtttccagtt accgccggct tcatctttat tcataatcaa gacctcatcg ttaggttgtg
    15541 aatgagagta cgttcacttt tcttctgaac gtgagattaa gtgtagtcag caatttggct
    15601 taggataatt tattcagaat ttttaaccgt cacgttgcgg caaaccaggt accgttgcgc
    15661 cagtgacgcc gccagatagc cgccagagaa agcccgcgca acgccagaaa gacggttaat
    15721 gccagccaca aaccatgatt acccagccac ggcagcgtaa ggagcgtcag cgcaaaacct
    15781 gcggcggcca ccgccatact gttacgcatt tcggcggcac gcgttgcgcc tataaacatg
    15841 ccgtccagca gataacacca gacgccaacc aacggcaaaa tcacctgcca gataagatag
    15901 cggtcagcca gctgctgaat ctgggttaac gacgtcagca acgcaatgat gtgttcccct
    15961 gccagcaaat aaaccaccga aaatagtaac gctacaatcc ccgactggcg gcacgctgcc
    16021 cgccatacat ccagcaactg gctaccgtcg cgcgcaccgt atgcctgacc ggagtgcgct
    16081 tcaaccgcgt aggcaaaacc atcaagcgca taggcggtaa aggtgagtag cgtcatcaga
    16141 accgcgttaa cagcgataat gtcactcccc agtcgcgcac caagtacggt gatcgcgccg
    16201 aaacagagtt gcaacaacag cgaacgcagc atgatatcgc ggttaagcgc cagcaagcga
    16261 cggaagtttc ctcgccaggc agttttcagc atttcgccgg aaattccgcg tagtttgagg
    16321 attttacgca ccattagcag accaatcagc aatgttgcat attccgcaat aaccgtcgcc
    16381 agcgccgcgc cctgcacgtt catatgcagc cccatcacca gccagacatc cagcacaatg
    16441 ttgaggatat tgccgaccac taacaaaatt actggcgcac gggcatattg cacgccgagt
    16501 aaccaaccga gtaataccag attcgccagc gacgccggag cgcttaacca gcggatttca
    16561 agaaagcgcc gcgcctgttc caggaccgct tcactaccgc caacaatatg cagcgccaga
    16621 tcgataatcg gcgtacgcaa cagcgcaatt aacgccccgg cccccaacgc caacagcaac
    16681 ggttgcacca gcgcacgggc taatgcctga ggatttttgg caccataagc ctgcgcagtc
    16741 agcccggtgg tgctcatgcg taaaaacagc aacagcataa agagaaagct ggtcgccgtt
    16801 gcaccaaccg ccacgccgcc caaataaacc ggactatcaa gatgaccaat taccgccgta
    16861 tcgaccagtc ccagcaacgg aacggtgata ttggagaaaa tcatgggtaa ggcgagatgc
    16921 cagagtgctt tatcagatga agtgaggaat gccatgcaga caagcctgat gaagagagat
    16981 gaaaaacaaa ccgcgacgcc aagcggcatc gcggtctcag agatatgtta cagccagtcg
    17041 ccgttgcgaa taaccccaac cgccagccct tcaatggtga agctctgctg acgaaggtca
    17101 acgacaattg gtttaaactc gctattttct ggcaacagtt cgactttatt gccctgtttt
    17161 ttcaggcgct taacggtaac ttcgtcatca atacgtgcga caacgacctg accgttacgt
    17221 acatcctgag ttttatgcac tgccagcaag tcgccatcca taatgccgat atctttcatc
    17281 gacatcccgc tgacgcgcag caggaaatca gcattcggct tgaacaagga aggatcgacc
    17341 tgataatgac cttcaatatg ctgttgcgcc agaagcggtt caccggcagc cacacgacct
    17401 accagcggca acccttcttc ctcttcctgc aacagacgaa tcccgcgtga tgcgccggaa
    17461 acaatttcaa taacgccttt gcgtgccagc gccttcagat gttcttcagc cgcgtttggg
    17521 gaacggaacc ccaaacgctg cgcgatttcc gcacgcgtcg gcggcatacc tgtctggctg
    17581 atgtgatcac ggatgagatc aaacacctct tgttgcctgg ccgttaacgc tttcattccg
    17641 ccccctgggt gtatatacag ttatactgtg agtatataca gcaaaaggcg attttggaac
    17701 cataaactgc acaataaacc agagatttat cgaaatctgg aagggttatc caaaatgcga
    17761 ccataacagg atgcaccagg taatcacggc gacgataatg gcaatcagca ccgcggcgga
    17821 tcccatatct tttgcgcgtc cggaaagctc atggtattca gagccaattc ggtcaaccac
    17881 tgcttcgatg gcgctattga ggatttccac aatcatcacc agcatcacgg agctgataag
    17941 cagcacgcgg gtaattgcgt ccacatccag ccagcaggcg atgaccaccg ccaacaatac
    18001 cgctacgcct tcctgacgga atgccgcttc gttgatccat gcagcgcgta aacctttcca
    18061 ggaatagcca gcagctttga taattcgggt gaatccagtt gtattattgg ccattgaaag
    18121 aaccttttta cattatgagc gtcaatatca gtgtaccgtt aaatccgatg attcgggttt
    18181 gcgtaacgcc gcgcgaaaca tgagcggata ccacagaatt tcccatgact ttctgctatc
    18241 cttgccgcgc atttgcatta ttaaccagag gctttacatc gtttatgtcc ggctggccac
    18301 gaatttacta caaattactg aatttaccat taagcatcct ggtaaaaagc aagtctattc
    18361 cggcagatcc tgccccggaa ctggggctgg atacctctcg tccaattatg tacgttttac
    18421 cgtacaactc gaaagcagat ttgctgacgt tgcgcgccca gtgtctggca catgatttgc
    18481 ctgacccgtt agagccgctg gaaatcgacg gcacgctact gccgcgctat gtgttcattc
    18541 acggcgggcc gcgtgtattc acctattaca cgccgaaaga agagtctatt aagcttttcc
    18601 acgactatct cgatttgcac cgtagcaacc caaatctgga tgtgcagatg gtgccagtat
    18661 cggtgatgtt tggtcgcgcg ccggggcgtg aaaaaggcga agtgaacccg ccgctgcgta
    18721 tgcttaacgg cgtacagaaa tttttcgctg tactgtggct cggtcgcgac agttttgtgc
    18781 gtttctcgcc gtcagtgtcg ctgcgccgta tggcggatga acacggcacg gataaaacta
    18841 tcgctcagaa actggcgcgc gtggcgcgta tgcactttgc ccgtcaacgt ctggctgccg
    18901 taggcccacg tctgcctgct cgtcaggatc tgtttaataa gctgctcgcc tcccgcgcca
    18961 ttgccaaagc ggtagaagat gaagcgcgca gcaaaaaaat ctcccatgaa aaagcgcagc
    19021 agaacgcgat tgcgctgatg gaagagattg cggcgaattt ctcttacgag atgatccgcc
    19081 tgactgaccg cattctgggc ttcacctgga accgatttta tcagggcatc aacgtccata
    19141 acgccgagcg cgttcgccag ctggcccacg acggtcatga gctggtgtat gtgccttgcc
    19201 accgcagtca catggactac ctgctacttt cttacgtgct gtaccaccag gggctggtgc
    19261 cgccgcatat cgccgccggg atcaacctga acttctggcc ggccgggccg attttccgcc
    19321 gtctgggggc gttctttatt cgccgtactt ttaaaggcaa taaactttat tccaccgttt
    19381 tccgtgagta tctcggcgaa ctgttcagcc gtggttattc cgtcgagtac ttcgtggaag
    19441 gcggtcgttc ccgcacgggg cgtttgctgg atccgaaaac cggtacgctg tcgatgacca
    19501 ttcaggcgat gctgcgtggc ggcacgcgtc cgattacgct gattccgatc tatatcggtt
    19561 atgagcacgt catggaagtg ggtacttacg ccaaagaact gcgcggcgcg acgaaagaga
    19621 aagagagcct gccgcagatg ctgcgcggtt taagcaagct gcgtaatctc ggtcagggtt
    19681 acgtcaactt cggtgaacca atgccgttga tgacctacct taaccagcac gtaccagact
    19741 ggcgtgaatc tatcgatccc atcgaagcgg tgcgtccggc ctggttaacg ccgacggtca
    19801 ataatattgc tgccgatctg atggtacgca ttaacaacgc aggcgcggca aacgccatga
    19861 acctgtgctg tactgcgcta ctggcatcac gtcagcgctc actcacccgc gagcagttaa
    19921 ccgagcaact caactgctac ctggatctga tgcgcaacgt accttactcc acggactcta
    19981 ccgttccttc agccagcgcc agcgagctta tcgatcacgc gctgcaaatg aacaagtttg
    20041 aagtcgagaa agacactatc ggcgacatca tcattctgcc acgcgagcaa gcggtgctga
    20101 tgacctacta tcgcaacaac attgcgcata tgttggtgct gccttcgctg atggcggcaa
    20161 ttgtcaccca gcatcgccac atctcccgcg acgtattgat ggagcacgtc aatgtgcttt
    20221 acccaatgct gaaagcggag ctgttcctgc gctgggatcg cgacgagttg ccggacgtta
    20281 ttgatgcgct ggcaaatgag atgcaacgtc aggggctgat taccctgcaa gatgatgagt
    20341 tgcatatcaa cccggcgcat tctcgcaccc tacagctgct ggccgcaggc gcgcgcgaaa
    20401 cgctgcaacg ttatgccatc accttctggt tgttgagtgc caacccgtcg atcaaccgcg
    20461 gtacgctgga gaaagagagc cgcaccgttg cgcaacgtct ctccgtgctg cacggcatca
    20521 acgcgccgga gttcttcgac aaggcggtgt tcagttctct ggtgctgacg ctgcgtgatg
    20581 aagggtatat cagcgatagc ggcgatgccg aaccagcaga aacgatgaag gtttatcagt
    20641 tgctggcgga gttgattaca tcagacgtgc gtttgacgat tgagagtgcg acgcagggcg
    20701 aagggtaatc agagagaatt gccggatgcg gcgaaaacgc cttatccggc ctaccatgac
    20761 ctgcaaattc aataaattgc gattcaccgg gtaggcctga gaagcgcaac gcatcaggca
    20821 acccagaaga aagccggatg atcatccggc ttttttacat catcagaaat gccagtaact
    20881 cattgccagt cctaaaaaca gtaccagacc aacatagtta ttattcataa atgctttaaa
    20941 gcaggcttca cgctcgcggt tggcaatcag tttttgttga taaacaaaca gcgcgccagc
    21001 caccacaatt gaccagtaat atccccagcc taagccattt aactcaccga tgatcgccat
    21061 cagtgccagt acgccaatct gcaaaatacc aataatcaat ttatcgtatt ggccgaacag
    21121 gattgccgtg gatttaatgc caatcttcac atcatcatcg cggtcaacca tcgcatactg
    21181 cgtgtcgtaa gccaccgccc agagaatatt ggcgaggaac attaaccagc aactcaatgg
    21241 caccgactca ctcacagcgg caaaagccat tggaatcgac cagccaaacg ccgcgcccag
    21301 caccacttgc gggagatggg tataccgctt cataaacggg tacacccacg ccaatgccag
    21361 agcggcaatc gacaacagaa tggtcatcgt attcagcgtc agaaccagta aaaacgaaat
    21421 cagtaccagc actacaaaca gcgcgcgcgc ctctttctct gttaccgcgc cgctgggaag
    21481 tggtcggttc gccgtgcgct taacatgacc atcaaacttg cgatcagcat aatcattcac
    21541 cacgcaacca gcagcgcgca tcagccagac acccgcgaca aacaccgcca tgatccagag
    21601 ctgaggaacg cccggtgtcg ccacccacaa cgcccataat gttggccaga gcagcagtaa
    21661 cgcgccaatt ggcttatccg tacgcattaa gcgatgaaac gccagcagct tattctgcgt
    21721 cagactccac tccatatttt tttcctctta gtacaacggt gacgccggta aaaacagttc
    21781 tgttagcaac agcggtttac cgcttaatcg caggcgggaa cgtcgccccc acagtccggc
    21841 atcacggcct atctcgataa aatcccgggt tagtgtcgat gatgtgaaca gatagcgtcc
    21901 taacggcgtt ttacccaatt tttgtaacgc tagttccggc ccgcttaatg ttgatacagg
    21961 aacgacggta cgaccggcaa gccacggttc accatcggca cataacaaaa tttcacgtaa
    22021 ccagtaacga gactctttcg gcagcagcgg cagttcttcg gggatttcat tctgctcgac
    22081 aaacccctcg cggatcatcg tcacactcac cgtttttccc tgctgttcaa aacgttttgt
    22141 catggaatcc tccagcaaca gccagtcgag cagttgcgga tccagggcag ggatctcttt
    22201 aaaatagcgc agcgcacgca gttgcgttaa cgcggggtgt gacatgccga actctccgtt
    22261 acataacgta aaggcattgt atcgcagaaa agtgtttacg ggagatgtaa agcaaattaa
    22321 gtgatctaat gcgcaacaaa aacgcaacaa tcgcatgaag tggtaaaacg ctgttgaacc
    22381 ttacgctttg ccgatgcccg tgctggcgag ggcaaccata ggggcatcac cgcccatgat
    22441 gctaggaacg gtgatgtttt cctttactta aataccttta ttctttatac gagtagctcg
    22501 ccaactcgca gtaaaaattg ggaaaagcgc atatttacca tgagatttgg agctgaaaag
    22561 tgtttcctga gtaagattaa taggcaacat cttatgtctg gatttataga tacacgtgga
    22621 attccaattg gtatcttttg taggattcaa catatcatgt aagcgctggg agcttaccac
    22681 cataacaggc ccatcatcga cagaggagaa caggataaat atattatcgt ttcgtgttaa
    22741 tttattagtg ttaaaggttc ttaacttttc ttcagtttct ataaatatag gtgaaacatc
    22801 atcactacga atccactctt tataaagata ccaggcattt gctttgatca tatcatccgt
    22861 tgatcgtaag gcctgatcga taagttggat aaaaaaattg ttatttttat caaccagccc
    22921 tttattcata ttaaatctgt taataaaatg gatcatctgt tgggtatcga aattcacctt
    22981 actgaatcga tgttgacgca taaaattatc gaacttgtca tcttccccag aatgtatata
    23041 actacgtata attggatctt ttatataagg acaatggcta aatgttttct tcatattctc
    23101 aataaagttt ttattaacaa tattgagaat ttttaattgc atcaccagtt ttactgccag
    23161 aacggatttc aatatcattg cattatctgg caatgtatca atcattttta taatgttatt
    23221 gatataacca tcgagatctt cattcccaaa tatatctgaa taatcaatag ttaattccaa
    23281 ttgttctgta atgcaatctt aaaaattccc tgtcgacatt cgcacatgat caaacataac
    23341 agctgtcaaa ttcgatttaa taaatgatat ttctttggca gacagaaact ctgctctggt
    23401 gaagtgcgaa gcgtaaagat aacaattatc gaagtttgaa tttctaatct gacattcgcg
    23461 aaaattacta tgaccaagtt gactctgcga gaagtcaact gtatcaagaa tattttggct
    23521 aaaatcaaca cattcgccac tcgttttgag aaaaatcaca ttgtgtaaac gtcgacattt
    23581 aaatatgaca ttatcaactg ttgccttagt aaaattgcaa ttctcaattt ctttacattc
    23641 gaaagttaca tgttgtagat ttgatgataa aaaagacacc ttagaaataa cgtcaccttt
    23701 aaaatgacaa tttgttaaat caaaatcaga caggtcgata tacccctgcg catctttatc
    23761 catcagaaaa ttacaagcgc attctatttt ttcatttctg tagaatttta cacttatagt
    23821 atcattacta attgagactt cacgtaacat atttattgca tgattttttt cgttccgttg
    23881 ttttatattt aaaaatatat cactgatata aataaagtaa tctctgttta aacctgtcac
    23941 cccattgaaa ttacgtagta aatcaagatc tactatatta tttccttgca ccacatcatg
    24001 tttcatcaaa gagttattgc cgccagaatt tgtgatatga cctatcataa aacctctgta
    24061 atacagaaat aaaataacct ggaaaagtag cctgagcgtc cagacactac cggcgaaagg
    24121 aaaagaatct ctgataatgc attgaaataa tggacattct tagtgaagaa gaagtgaagt
    24181 actataaaaa atcgctcata tcccaggaag gaagcataaa aaaaggtgcg ccaggagacg
    24241 caccagttgt tgcaaaatca gcactgtggg gcgtaattac cccttgcctt ttacactgct
    24301 gataaaggtg gaacgggcag atgtcgatcc caggcgttca gcttcatcaa gcagttttaa
    24361 cgccttatca acatcacctt tcgcgacagc gtttttgatc gcggtattaa aataactttc
    24421 cgtgtcgttg agcatcggct cgcttttctt caccggcacc ggagcaggtg cagccacagc
    24481 tggtgccgcc gtgttgccta ccgtaaccgg agctggagcg gaagaaccaa atagcggtcc
    24541 taccaacacg ctggagctgg agttcgtttt gactttcagt ttcagtaagc catcgctggt
    24601 atgacgagca accggatcgg gaatatccgg gatcgagtta ccgacgcctt tggcataggc
    24661 tttagccggg tcgagcagtt gggtcgtctg ctggagatct ttttccgtgg taaagaccag
    24721 aacataaagt ttttgctgcc ccaacgccgg tgtcaggcgc ataacgcctt ccagccgatc
    24781 tgcactcatc acgcctagtt cctggtaggt gaaataactg ctggggaaga aggctgatgg
    24841 ggtcatgttc tgatcaagaa tcagcacgtt cggcgcaaaa acgctggttt gtttgttcac
    24901 ttcgctggtc agcgtcaggg tcagttcgcc aatgtttgcc gggacgctgt acgcagcaac
    24961 cggaccactg atgccgggaa cgttcagttg ttggccgccg gtcgccagtt gggtggtctg
    25021 ggttttagat tgatcgaccg gtgtccaggt gagttgttgc agcgcggcag atggaatggc
    25081 tggcgcgtcg ctggtgtttt gcggtacgta gttaacatcg gcaaggctaa ttccaggcgc
    25141 gcttgccagt aaccctgctg ataaacagag ggcgatgaga cttttattca ttttcattgt
    25201 tttcacctca aaatctggag ctcagcggta gccaggcaat agcgcgctaa acccgataat
    25261 cagaggggct ttcgcccctt tcagataatg acaacctgtt tttatgccgg atgcggcgta
    25321 aacgccttat ccggcccagg ttttgctatt accaccagat ttccatctgg gcaccgaagg
    25381 tccactcgtc gctgtcgcca cgaccgaagc tgccgccgtt gaaatcagca ggaacggctt
    25441 tgccgtagtt cgggttaacc ttgctatcgc cgttgtagtc gtaaccccat ttctcatccc
    25501 acttggcgta ggttgcgaag acacgaatag ccgggcgtga ccagatgctg tcgccagcct
    25561 gccattgttg tgcgagggta attttgtact gattgttctt gtcgccggtg cgctgggatt
    25621 cgacgttgtc gtagccgatt tccatcacgg tgctcatgat tggcgtccac ttgtacatcg
    25681 ggcgaatacc aacggtccac cacttggtgc cgttgtcgtt atcccagttg atatcctggt
    25741 acatacccac gtacatcatg tcccagttgt cgcccatgga gatcgcaccg tggtcgagga
    25801 tacgcagcat gtgaccgttg ttgttgatat tgtaggcaaa tttttcgtta tcaaacgcga
    25861 cgccagaacc ctgcgacaga cctttaccct gcgaggtcat cgagtcagta gcgtactgaa
    25921 caacaaactt gttaaagccc ttcaggacac tctgagtatg ttcagcagtg aataaccagc
    25981 cgtctttcga tgcgccatca accagacgat agttatcacg caggttggca cgaccgtagt
    26041 cgacacccag ttctaatgtg ccgcccgggt tgatttccat ctgcgctaaa cgcacatcga
    26101 aaacgtcgtt cgcggtttcg ttggtatagt cataaatatt gttgctggca aaagaggaag
    26161 aaccaccagc ttcagaggag cgggttgctg ccagagagag tttaccgaag ccaacatcga
    26221 tgttttccag accggcacca ggaccagaaa tatcccagta gtagaagtcg atcatatgaa
    26281 cgtcatgacg ttggtagaag cgcttacctg cccatatggt ggaacctggc agccattcga
    26341 tcaggttttt tccctgcacg tttgcttcac ggaaggccgg atcggtagct tcccagtcat
    26401 tctgttgcgc gacggaatag gccacgttag tgtcgaaata gaagctctta tcgccctctt
    26461 tccacacttc ctgacccaat tttaattcag cataagtttc acattcgttg ccaagacggt
    26521 atttactttg agcaccggta gtctggaaac actgttgttc accgccgcta cctgtccagc
    26581 caataccgga acgtgcatag ccgtggaaat caacagccat tgcctgagca gacattacgc
    26641 ccgctgcgac ggcaaccgcc agaggaagtt tgcgcagagt aatcatcatt ctatctcctg
    26701 agtcattgct tttctttttt cacatcacct gtgacaggct ttgtgtgttt tgtggggtgc
    26761 ttaaacgccc ggctccttat gcagtcgacg acatgcagtg ccatcctcac ggaacagatg
    26821 gcaacgctct ggcggcaggc cgatagcgaa tgtggcacct tcttctacca acaccacgtc
    26881 gttctggcgg tacaccaggt tttgacgaat ggaagggatc tggatataga tttgggtttc
    26941 gttgccgagt tgctcgacga cctgaacttc accctcaagg atgacgtcag cgatatcact
    27001 cggcagtaga tgttccgggc gaatacccag cgacatattg gctccaacct ggacatcacg
    27061 gctttcaaca ggcagccaga cttgttgacg atttggcatc ggcagctcca cctgcacttg
    27121 atcgattgcg gtggcggtca cttttaccgg caggaagttc atctttggcg aaccgataaa
    27181 tccggcgaca aaacggtctg ccggatagtg gtacagttcc agcggtttcc caacctgtgc
    27241 cacgcgaccg gcgtccagca ccacgatttt gtcggccagc gtcatcgctt cgacctgatc
    27301 gtgggtgacg taaatcattg tgcggcccag gcgtttatgc agacgggaga tttcgatacg
    27361 catttgcaca cgcagtgcag catcgaggtt ggagagcggt tcatcgagca aaaatacgct
    27421 tggctcggcc accagcgtac ggccaatcgc cacgcgctga cgctgaccac cggagagcgc
    27481 tttcggtttg cgatccagca aatgcgccag ttgcagcact tccgccacct ggttgacgcg
    27541 ttggttaatc acctcttttt ttgcgccagc cagtttcagg ccaaatgaca tgttttctgc
    27601 tactgacagg tggggataga gcgcgtaaga ctgaaacacc ataccaacgc cgcgttctgc
    27661 tggcggagtg tcattcatcc gtttctcacc gatgaacagg tcgccgctgg tgatcgtctc
    27721 aagcccggca atcatgcgca gtaaagtcga tttaccgcag ccagacggtc cgacaaacac
    27781 cacgaattca ccttcatgga tatcgagatt gatatctttc gataccacga cctcgcccca
    27841 ggctttcgtt acattttgca gctgtacgct cgccatgccc ttctcccttt gtaacaacct
    27901 gtcatcgaca gcaacattca tgatgggctg actatgcgtc atcaggagat ggcttaaatc
    27961 ctccaccccc tggctttttt atgggggagg aggcgggagg atgagaacgc ggcttctgtg
    28021 aactaaaccg aggtcatgta aggaatttcg tgatgttgct tgcaaaaatc gtggcgattt
    28081 tatgtgcgca tccccacatt accgccaatt ctgtaacaga gatcacacaa agcaacggtg
    28141 gggcgtaggg gcaaggagga tggaaagagg ttgccgtata aagaaactag agtccgttta
    28201 ggtgttttca cgagcacttc accaacaagg accatagatt atgaaaataa aaacaggtgc
    28261 acgcatcctc gcattatccg cattaacgac gatgatgttt tccgcctcgg ctctcgccaa
    28321 aatcgaagaa ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc
    28381 tgaagtcggt aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga
    28441 taaactggaa gagaaattcc ctcaggttgc ggcaactggc gatggccctg acattatctt
    28501 ctgggcgcac gaccgctttg gtggctacgc tcaatctggc ctgttggctg aaatcacccc
    28561 ggacaaagcg ttccaggaca agctgtatcc gtttacatgg gatgccgtac gttacaacgg
    28621 caagctgatt gcttacccaa tcgctgttga agcgttatcg ctgatttata acaaagacct
    28681 gctgccgaac ccgccaaaaa cctgggaaga gatcccggcg ctggataaag aactgaaagc
    28741 gaaaggtaag agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat
    28801 tgctgctgac gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt
    28861 gggcgtggat aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa
    28921 caaacacatg aatgcagaca ccgattactc catcgcagaa gctgccttta ataaaggcga
    28981 aacagcgatg accatcaacg gcccgtgggc atggtccaac atcgacacca gcaaagtgaa
    29041 ttatggtgta acggtactgc cgaccttcaa gggtcaacca tctaaaccgt tcgttggcgt
    29101 gctgagcacc ggtattaacg ccgccagtcc gaacaaagag ctggcgaaag agttcctcga
    29161 aaactatctg ctgactgatg aaggtctgga agcggttaat aaagacaaac cgctgggtgc
    29221 cgtagcgctg aagtcttacg aggaagagtt ggcgaaagat ccacgtattg ccgccaccat
    29281 ggaaaacgcc cagaaaggtg aaatcatgcc gaacatcccg cagatgtccg ctttctggta
    29341 tgccgtacgt actgcggtga tcaacgccgc cagcggtcgt cagactgtcg atgaagccct
    29401 gaaagacgcg cagactcgta tcaccaagta atgctgtgaa atgccggatg cggcgtgaac
    29461 gccttgtccg gcctacaaaa ctgaaacgca tgtaggcctg ataagacgca tcagtgtcgc
    29521 atcaggcagt tgttgtcgga taaggcgtga aagccttatc cgtcctggaa tgaggaagaa
    29581 ccccatggat gtcattaaaa agaaacattg gtggcaaagc gacgcgctga aatggtcagt
    29641 gctaggtctg ctcggcctgc tggtgggtta ccttgttgtt ttaatgtacg cacaagggga
    29701 atacctgttc gccattacca cgctgatatt gagttcagcg gggctgtata ttttcgccaa
    29761 tcgtaaagcc tacgcctggc gctatgttta cccgggaatg gctggaatgg gattattcgt
    29821 cctcttccct ctggtctgca ccatcgccat tgccttcacc aactacagca gcactaacca
    29881 gctgactttt gaacgtgcgc aggaagtgtt gttagatcgc tcctggcaag caggcaaaac
    29941 ctataacttt ggtctttacc cggcgggcga tgagtggcaa ctggcgctca gcgacggcga
    30001 aaccggcaaa aattacctct ccgacgcgtt taaatttggc ggcgagcaaa aactgcaact
    30061 gaaagaaacg accgcccagc ccgaaggcga acgcgcgaat ctgcgcgtga ttacccagaa
    30121 tcgtcaggcg ctgagtgaca ttaccgccat tctgccggat ggcaacaaag tgatgatgag
    30181 ctccctgcgc cagttttctg gcacgcagcc gctctacaca ctcgacggtg acggcacgtt
    30241 gacgaataat cagagtggcg tgaaatatcg tccgaataac caaattggct tttaccagtc
    30301 cattaccgcc gacggcaact ggggggatga aaagctaagc cccggttaca ccgtgaccac
    30361 cggctggaaa aactttaccc gcgtctttac cgacgaaggc attcagaaac cgttcctcgc
    30421 cattttcgtc tggaccgtgg tgttctcgct gatcactgtc tttttaacgg tggcggtcgg
    30481 catggttctg gcatgtctgg tgcagtggga agcgttgcgc ggcaaagcgg tctatcgcgt
    30541 cctgctgatt ctgccctacg cggtgccatc gttcatttca atcttgattt tcaaaggatt
    30601 gtttaaccag agcttcggtg aaatcaacat gatgttgagc gcgctgtttg gcgtgaagcc
    30661 cgcctggttc agcgatccga ccaccgctcg cacgatgctg attatcgtca atacctggct
    30721 gggttatccg tacatgatga tcctctgcat gggcttgctg aaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gccttttttt ctttcgtctg caatctgaat cgttcgccgg
    32161 ttaatattcc catcatagaa cttattattt ttacgttatt tgttttccca cttacgataa
    32221 ttctctttcg tgctctgagt cacggcaata gtattgtttc atcaattttt gataattatc
    32281 acaattaaga tcacagaaaa gacattacgt aaacgcattg taaaaaatga taattgcctt
    32341 aactgcctga caattccaac atcaatgcac tgataaaaga tcagaatggt ctaaggcagg
    32401 tctgaatgaa tacccagtat aattccagtt atatattttc gattacctta gtcgctacat
    32461 taggtggttt attatttggc tacgacaccg ccgttatttc cggtactgtt gagtcactca
    32521 ataccgtctt tgttgctcca caaaacttaa gtgaatccgc tgccaactcc ctgttaggat
    32581 tttgcgtagc cagcgctctg attggttgca tcatcggcgg tgccctcggt ggttattgca
    32641 gtaaccgctt cggccgtcgt gattcactta agatttctgc tgtcctgttt tttatttctg
    32701 gtgtaggttc tgcctggcca gaacttggtt ttacctctat aaacccggac aacacagtac
    32761 ctatttatct ggcaggttat gtcccggaat ttgttattta tcgtattatt ggcggtattg
    32821 gcgttggttt agcctcaatg ctttcaccaa tgtatattgc ggaactggct ccagctcata
    32881 ttcgcggaaa actggtttca tttaaccagt ttgcgattat tttcgggcaa cttttagttt
    32941 actgcgtaaa ctattttatt gcccgttccg gtgatgccag ctggctgaat actgacggca
    33001 ggcgttatat gtttgcctcg gaatgtatcc ctgcactgct gttcttaatg ctgctgtata
    33061 ccgtgccaga aagtcctcgc tggctgatgt cacgcggcaa gcaagaacag acggaaagta
    33121 tcctgcgcaa aattatgggc aacacgcttg caactcaggc agtacaggaa attaaacact
    33181 ccctggatca tggccgcaaa accggtggtc gtctgctgat gtttggcgtg ggcgtgattg
    33241 taatcggcgt tatgctctcc atcttccagc aatttgtcgg catcaatgtg gtgctgtact
    33301 acgcgccgga agtgttcaaa acgctggggg ccagcacgga tatcgcgctg ttgcagacca
    33361 ttattgtcgg agttatcaac ctcaccttta ccgtactggc aattatgact gtggataaat
    33421 ttggtcgtaa gccactgcaa attatcggcg cactcggaat ggcaatcggt atgtttagcc
    33481 tcggtaccgc gttttacact caggcatcgg gtattgtggc gctactgtcg atgctgttct
    33541 atgttgccgc ctttgccatg tcctggggtc cggtatgctg ggtactgctg tcggaaatct
    33601 tcccgaatgc tattcgtggt aaagcgctgg caatcgcggt ggcggcccag tggctggcga
    33661 actacttcgt ctcctggacc ttcccgatga tggacaaaaa ctcctggctg gtggcccatt
    33721 tccacaacgg tttctcctac tggatttacg gttgtatggg cgttctggca gcactgttta
    33781 tgtggaaatt tgtcccggaa accaaaggta aaacccttga ggagctggaa gcgctctggg
    33841 aaccggaaac gaagaaaaca caacaaactg ctacgctgta atcttcctgt ccagcacgcc
    33901 gcgccatttc ggcgtgctgt ctttttactc ccgcttcagc cgtttcgaat tacacagcca
    33961 cagggtgatc accagtaaca ggatcgcagc cgagtagatc aacacatcca gtggcgattt
    34021 atgatcgacg atgatcaagc gcacaatggc ggtgatccca atatagacaa agtaacgtag
    34081 cgggaagtga aaaccggact gaaagtactt cacaatcagc gcgataaatt cgaaatagag
    34141 aaagtaaacc accagtcctt ctaccagctc atatttgctg gtttgttctg gcgcgaacag
    34201 cacatcagcc agatgcaccg tttctttgcc gaggaagaca accaaaatca ggccgaggca
    34261 cagcaggcca agattgagta cggtctgcaa aatggtggag ataaactcca cgcgcggacg
    34321 agagagtgac gtcatagcag tacctccttc acagtggcga gcttcctgta taacgcaaaa
    34381 atgtgacgga gatctatatt ttttgtgtat gttttgttca acctggattt ggagcgcgcc
    34441 tccaccaaag ccggatatac ctacccgtag gccggataag ccgcgcctgg cggcgcatcc
    34501 ggcaagacaa accgcaccaa agccggtaat tcaaaaatca cccattcagg tgattcccag
    34561 ccaccagcaa tgcgcgaaaa atcgacaatg ttcctctgga acaaatgaaa cgtaacctga
    34621 taaaggtggt aaaaatgaag ccttattttg ctgctttgat gttatcagtc tctgttcttc
    34681 ctgcttatgc tggtccgctc ggtactgctg ataaagccga cctaccgcaa agcaatgtct
    34741 ccagcccaat gatggcccag tcgctcaggc aaccagacct gcaaccgata tcgacggaca
    34801 ggaagacaga atgttttcgc ctttacacac cagatcgtaa accgggggtg aactgcgttc
    34861 ctgatggctc aacaggtcat taagaatgag ggtgccagtg agtgcgaaaa ctggcacctt
    34921 tcccatgact tagcggaaat ttacgctacg gtcgctggtc atgtcataca actggaactt
    34981 acgcccaagt tgctgaccac cgtcacgcgt cagcggcgtc cagccaatcg ccgcacggct
    35041 gcgtgtcggg ccagacgaga agagatccaa cggtaccgag acatacacgc ctttggtgaa
    35101 gtccccttcg ccgtactcct ctttcgaaac gttggtgatc gtggcatagc cacccaccac
    35161 cacgccgctg tcaaagcgtt tagcgatctc cagcgtgccg cctttatccc ctgccagata
    35221 ctgcccgacg ctggctttca ccaacacgtc ctgggcgaaa gatggcgtcc agtaggcggt
    35281 cagatgtccg gttttcacgc tgtagtcggt gaatttcatc atatcttttg cactacgcca
    35341 gtcgcgctgt ttaacgtagt tggcatccag accaaacgcc cagttactgt ccagcggacg
    35401 atacaacact tcggcccccg caccgccaaa catggtttcg agataacctc catagacctg
    35461 accgtagaag ccgttgccca gatgctggaa gtagttggct tgcaggttat tcacatagac
    35521 atcgttctgc acatactcgc gcacatgggt acgaacgcgc ggtaagtgcg agtcctgcgg
    35581 tggattggtg tagttaaact tgtcgtagtt gttggcaaga tttgcaaaca ggctgccggt
    35641 ggtcagtaga tggtccgtca gccacaaatc tgccgttccc atcacgccca gctgatacat
    35701 gtaaaagttt tccgggccac cgaccgactg gttcagcacc ggatcgatat ggaaatcaaa
    35761 gcgtgatttg tcgatatacc agccctgctc ggtggacttc ggaaccactg gctcgacgcg
    35821 tttgtgcgcc agcgtcgttt cgtggcccaa cggctctccg gcgagatgat ttttcaggct
    35881 ggcgacatcg gtttccgtcg tcacttgcgg catgttaagg cgattttccg taatgcggat
    35941 cgttttgatc ccatccggca gatcgttcat cacgatccga ttagcacgga tgatcccttc
    36001 gcgcgaatca cgatatttca cctgctcgcc ggtaacatac agcgtatcgc ctttcgcctg
    36061 gatctgcgga tcggcaagtc cggcgttgta tttcagcaac gttaactgat tcgccaccac
    36121 cgaatgctgc aaaatggcat cctgcggctg cggttgatat tgcgggcggg cgttatcgat
    36181 gtaggacggg cgcagatcgt taaagttggt gcgcagcgtg acgccaaaca taaaggtgtt
    36241 gccacgttca tagctaaggt taacgtcggc ccaatcggta acgcgataaa tcgccccgac
    36301 gttaaactta cttttttgct ccagcttccc ggcaaaatcc tgctgataat tattaccttc
    36361 atactccagt ttcagacgca gcggttgcca gggcgtctgg tattccacgc cgccaaacag
    36421 tgaggcagga ccgtggaaca tctggctacc gtcgatagat cccgcctgtt tgtagctgtt
    36481 atcgcgatag caatatttat cactggctga acagagcgga ttttttacat taccgctggt
    36541 gcccagatat ccccagccca ggccgagcgt aaaatcgaac ggcccccagg ctttgctggc
    36601 aacaagatat tccgcatcaa acagccccgt accgccgata tcccgcgcac caaccgccac
    36661 ttgcggcagc cagtaactct cttcccacaa acgcagtttg agatcgaagg ctttatcttt
    36721 atacgtttga tcgccagaga acgcttcgac gctgctgtac tgccgggtgc gcacgtcggt
    36781 gtagcgcagc gttgtttcca gccacgggaa gagttgcact gaagctgagt aataacggta
    36841 ctgatcgtta tcgcgatagt tcagactcaa ctccccttcc cgtgccatac gcgcggtggg
    36901 agtttgtaat aatcctacgc caccgaaatc cgactgcgac ggaccaatgg gcgcaggata
    36961 tgtttcgccg tagcaagctg tgctaatgcc cagcgccagt aagctaagca gatgtctttt
    37021 tttcattatt ggggtatccg ctgcgtcaga gtctgaagaa tgtcggcgtt aagggcatca
    37081 ggcgtctcac tccagacgga atctgcgagg ccaacataaa taatgctgcc gggcatcggc
    37141 tctacgtgac gcttgttcca gtaagccacc ggcgcttttt gcgtgcgtcc gtccgggtaa
    37201 acgacccacg cgtagctgcg atccgcaccg ctgagcaggc tctggtcaga gagatagctc
    37261 gccacgtcgc gacctggagt gaatggctga ttgccaggat ggctgataag cccgaacaac
    37321 gtgaccgtgg acggcggtgg tccgacccac agcgtgtagt tgccctgcaa cggcgggtta
    37381 ccgcgttcgg caacgcggac gatatcggga tcaagattga ttttttgcct gcctgtcact
    37441 ttcaatgcct ggatttgctg gcgtaaggcg ttaatggcgg cggcatcgtc ggtgctggaa
    37501 tctgcgccct gttctgccag ccgtgtcagc aacgcctgct gctgacgcaa tgctgccgcc
    37561 gttgccagct cttcgctaat caccgcacca ggccaccagc tgtttgccag tcgaggttgc
    37621 cctaccagat cgattaaatg ctctgcgccc gttagcgttt tggcctcact gctgccattg
    37681 ctgaacacct tgacggttcc tgccgcgaag accgatgacg ctcccacact caaaagcaac
    37741 gcgacaatag tttgtttaat cattgttttg ccgccttgat gagagtggtt ttcaccggga
    37801 aataatccgc gccgagatac tgttccgact ggcgaatttg cccttcgctg tcgatccagt
    37861 agcggttatg ccagcgcgcc tggtcggtgg agacttcttc gtccagcacg cgaaccaggg
    37921 tttcatcgct gccgaccttg acggtatcgg tgccatccca tttgaagact gagcgtgcgg
    37981 tggcgtagcg tacctgctgg tactcggtcc agcccatcgt gcgcgtccat gttgcaccat
    38041 caacaatttg tgcgggttta atcagcgggt cggcggcaag gttattcact tcaatcaggt
    38101 tgtcgccgcc aagcaaagtc ttcaccagac gaccatgttg tgtgacaaga gtggcttgat
    38161 cctgagtgac ccatttttgt tgtccgtctt cagcgaaggc cagcaccaca aacaattgcg
    38221 gcccgccatt aagctgcatg tactggctgg cgtagggcat attttgaata tcatcgtccg
    38281 tcagctgtac gcctggcgtg ccgaacagac tgtcccacag tgaattgccc agctctttag
    38341 tcgtggctga acaggcctgt aatagcaggc agataagaat gagtgcaggt cgcttcacga
    38401 ctcttctcca gaggggcaga ataaccacac cggagtgtgg ttatggtaaa tcccttcata
    38461 cttcaagcca cagatgcaat gactcaaatt attttgggga tacatacttt attactgggt
    38521 actggtggtc gtggttgtgg tggtcccggt attagaacca tcaccgccac cggttgccgc
    38581 gagcgcgaca cccaccgccg agcttacggt gctgacgctg gtcgcggagg aactccccgc
    38641 cgaaaccgac gttgctgccg actctgccgc ttcgcccacc tgtacaggtg cagcccacgc
    38701 agaagtcgcc gcaagcgcag atatggcaaa aatgccatac agaacttttt tcataacaat
    38761 ttcccttcat tgaatgaatg gagatttacc caaaaacatt tcgggcggga tcgagtatac
    38821 acaactaaag catgcgaatt acggcgcggg gaattagcga tggagtttta ggataattgg
    38881 aatgaatatc aaatataagt ttcaacttat atttaaataa caataaaaat cataagctta
    38941 aaataaaaaa caaaagtaat taatctgcgt attctccgaa ataaacaacg attaaaaatc
    39001 tattttagga ttaacctcac ggtatgattt ccgttaaatc acagacaagg ggatttatct
    39061 gataaaacaa tgccgactta ttgtcggcat tgtttttaag gttatttaac tcctcatact
    39121 atgcattaat gtcgatattg gcatcaggct ccggttgccg gatgcggcgt aaacgcctta
    39181 tccggcctac atatcggcat caggcacgca tgatgattaa ccgcgccacg ctttatagcg
    39241 gttaatcaga ccattggtcg agctatcgtg gctgctgatt tctttatcat ctttcagctc
    39301 tggcagaata cggtttgcca gttgtttacc cagctccacg ccccactggt cgaaggtgaa
    39361 gatgttcagg atcacgccct gagtaaagat tttgtgctca tacagcgcaa tcaacgcacc
    39421 caggctgaac ggagtgattt cgcgcagcag gatggagttg gtcgggcggt taccttcgaa
    39481 cactttgaac ggcaccacgt agtcaagcgt tgccggatct ttaccctgat cgcgatattc
    39541 ctgctcaacc acttcgcggg atttaccaaa cgccagcgct tcggtctggg cgaagaagtt
    39601 agacagcagt ttctggtggt gatcagagag cgggttatgg gtgatagccg gggcgatgaa
    39661 atcgcacggt accattttgg ttccctggtg gatcagctgg tagaacgcgt gctgaccgtt
    39721 agtgcctggt tcaccccaga taatcgggcc agtctggtaa tccacaacgt taccgttacg
    39781 gtcaacatac ttaccgttgg actccatatt gccctgctgg aagtacgccg cgaaacggtg
    39841 catatactgg tcatacggca gaatcgcttc agtttccgca ccaaagaaat tgttgtacca
    39901 gatgccaatc agcgccagca ggacaggcag gtttttctcg gcaggcgtgg tggagaaatg
    39961 cttgtccatt gcgtgtgcgc cagaaagcag ttcaacgaag ttatcaaagc cgatggagag
    40021 aacaatcgac aggccaatcg ctgaccacaa agagtaacgg ccgccgaccc agtcccagaa
    40081 ctcgaacatg ttggcagtat caataccaaa ctcgccaacg gctttggcat tggttgagag
    40141 cgccgcaaaa tgtttcgcaa cgtgtttttc atcacctgcc gctttcagga accagtcacg
    40201 cgcgctatgg gcgttggtca tggtttcctg agtggtgaag gttttagatg ctaccaggaa
    40261 cagcgtggtt tccgggttta cttttttcag cacttccgag atgtgagtcc catcgacgtt
    40321 agaaacaaag tgcatgttca ggtggttttt gtacggacgc agtgcttcgg tcaccatgta
    40381 cgggccgagg tcagaaccgc cgatcccgat gttcactacg tcagtgattg ctttgccggt
    40441 ataacccttc cactcaccgg agataatcgc ttccgagaag gttttcatct tctccagcac
    40501 cgcgttgact tctggcataa catctttgcc atcaaccaga atcggggtat tgctacgatt
    40561 acgcagcgct acgtgcagca cggcgcggtt ttcagtgcgg ttgatcttct cgccagagaa
    40621 catcgactta atcgcgcccg ccagatcgca ctctttcgcc agatcctgta atttcgccag
    40681 cgtctcttca gtgatgcggt ttttggagta atccaccagc atctgatcgt cgaaggttgc
    40741 ggagaactta gaaaaacgat cgccgtcttt agcaaaaaga tcggcgatcg taacgtcttt
    40801 catttcatcg aagtgtttct gtagtgcctg ccaggcagcg gtctgcgttg gattgatgtt
    40861 tttcattagc aatactcttc tgattttgag aattgtgact ttggaagatt gtagcgccag
    40921 tcacagaaaa atgtgatggt tttagtgccg ttagtgtaat gttgagtgta aacccttagc
    40981 gcagtgaagc atttattagc tgaactactg accgccagga gtggatgaaa aatccgcatg
    41041 accccgtcgt tgacaaccgc cccgctcacc ctttatttat aaatgtacta cctgcgctag
    41101 cgcaggccag aagaggcgcg ttgcccaagt aacggtgttg gaggagccag tcctgtgata
    41161 acacctgagg gggtgcatcg ccgaggtgat tgaacggctg gccacgttca tcatcggcta
    41221 caggggctga atcccctggg ttgtcaccag aagcgttcgc agtcgggcgt ttcgcaagtg
    41281 gtggagcact tctgggtgaa aatagtagcg aagtatcgct ctgcgcccac ccgtcttccg
    41341 ctcttccctt gtgccaaggc tgaaaatgga tcccctgaca cgaggtagtt atgtctgaaa
    41401 ttgttgtctc caaatttggc ggtaccagcg tagctgattt tgacgccatg aaccgcagcg
    41461 ctgatattgt gctttctgat gccaacgtgc gtttagtagt cctctcggct tctgctggta
    41521 tcactaatct gctggtcgct ttagcggaag ggctggaacc tggcgagcga ttcgaaaaac
    41581 tcgacgctat tcgcaacatc cagtttgcca ttctggaacg tctgcgttac ccgaacgtta
    41641 tccgtgaaga gattgaacgt ctgctggaga acattactgt tctggcagaa gcggcggcgc
    41701 tggcaacgtc tccggcgctg acagatgagc tggtcagcca cggcgagctg atgtcgaccc
    41761 tgctgtttgt cgaaatcctg cgcgaacgcg atgttcaggc acagtggttt gatgtacgta
    41821 aagtgatgcg taccaacgac cgatttggtc gtgcagagcc agatgtagcc gcgctggcgg
    41881 aactggccgc gctgcagctg ctcccacgcc tcaatgaagg cttagtgatc acccagggat
    41941 ttatcggtag cgaaaataaa ggtcgtacaa cgacgcttgg ccgtggaggc agcgattata
    42001 cggcagcctt gctggcggag gctttacacg catctcgtgt tgatatctgg accgacgtcc
    42061 cgggcatcta caccaccgat ccacgcgtag tttccgcagc aaaacgcatt gatgaaatcg
    42121 cgtttgccga agcggcagag atggcaactt ttggtgcaaa agtactgcat ccggcaacgt
    42181 tgctacccgc agtacgcagc gatatcccgg tctttgtcgg ctccagcaaa gacccacgcg
    42241 caggtggtac gctggtgtgc aataaaactg aaaatccgcc gctgttccgc gcgctggcgc
    42301 ttcgtcgcaa tcagactctg ctcactttgc acagcctgaa tatgctgcat tctcgcggct
    42361 tcctcgcgga agttttcggc atcctcgcgc ggcataatat ttcggtagac ttaatcacca
    42421 cgtcagaagt gagcgtggca ttaacccttg ataccaccgg ttcaacctcc actggcgata
    42481 cgttgctgac gcaatctctg ctgatggagc tttccgcact gtgccgggtg gaggtggaag
    42541 aaggtctggc gctggtcgcg ttgattggca atgacctgtc aaaagcctgc ggcgttggca
    42601 aagaggtatt cggcgtactg gaaccgttca acattcgcat gatttgttac ggcgcatcca
    42661 gccataacct gtgcttcctg gtgcccggcg aagatgccga gcaggtggtg caaaaactgc
    42721 atagtaattt gtttgagtaa atactgtatg gcctggaagc tatatttcgg gccgtattga
    42781 ttttcttgtc
//
LOCUS       DADLTY010000011        38786 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.011, whole genome shotgun sequence.
ACCESSION   DADLTY010000011 DADLTY010000000
VERSION     DADLTY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.011"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="JN334_000510"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="JN334_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HAY7436770.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            530..571
                     /gene="hisL"
                     /locus_tag="JN334_000511"
     CDS             530..571
                     /gene="hisL"
                     /locus_tag="JN334_000511"
                     /inference="COORDINATES: protein motif:HMM:NF019659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HAY7436771.1"
                     /translation="MTRVQFKHHHHPD"
     gene            717..1616
                     /gene="hisG"
                     /locus_tag="JN334_000512"
     CDS             717..1616
                     /gene="hisG"
                     /locus_tag="JN334_000512"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAY7436772.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1622..2926
                     /gene="hisD"
                     /locus_tag="JN334_000513"
     CDS             1622..2926
                     /gene="hisD"
                     /locus_tag="JN334_000513"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAY7436773.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2923..3993
                     /gene="hisC"
                     /locus_tag="JN334_000514"
     CDS             2923..3993
                     /gene="hisC"
                     /locus_tag="JN334_000514"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAY7436774.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3993..5060
                     /gene="hisB"
                     /locus_tag="JN334_000515"
     CDS             3993..5060
                     /gene="hisB"
                     /locus_tag="JN334_000515"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HAY7436775.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5060..5650
                     /gene="hisH"
                     /locus_tag="JN334_000516"
     CDS             5060..5650
                     /gene="hisH"
                     /locus_tag="JN334_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAY7436776.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5650..6387
                     /gene="hisA"
                     /locus_tag="JN334_000517"
     CDS             5650..6387
                     /gene="hisA"
                     /locus_tag="JN334_000517"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAY7436777.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6369..7145
                     /gene="hisF"
                     /locus_tag="JN334_000518"
     CDS             6369..7145
                     /gene="hisF"
                     /locus_tag="JN334_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAY7436778.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7139..7750
                     /locus_tag="JN334_000519"
     CDS             7139..7750
                     /locus_tag="JN334_000519"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAY7436779.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="JN334_000520"
     CDS             complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="JN334_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HAY7436780.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="JN334_000521"
                     /pseudo
     CDS             complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="JN334_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="JN334_000522"
     CDS             complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="JN334_000522"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAY7436781.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11944..12303)
                     /locus_tag="JN334_000523"
     CDS             complement(11944..12303)
                     /locus_tag="JN334_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436782.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12300..12851)
                     /locus_tag="JN334_000524"
     CDS             complement(12300..12851)
                     /locus_tag="JN334_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436783.1"
                     /translation="MIAKKRNIQTFLIFSLATILGGIYWYVRSFWISGDPFSPAGGNI
                     FGHYLWNEIDLQVQTAEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKIL
                     WVFFSIVLTYVLFWFSVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFI
                     LVCIIFFRGDRCILNSNIFILNT"
     gene            complement(12952..13551)
                     /locus_tag="JN334_000525"
     CDS             complement(12952..13551)
                     /locus_tag="JN334_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436784.1"
                     /translation="MSLNILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLA
                     INNIEFSTLFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYL
                     RFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIAT
                     ILLFTINPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13722..14660)
                     /locus_tag="JN334_000526"
                     /pseudo
     CDS             complement(13722..14660)
                     /locus_tag="JN334_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14723..15097)
                     /locus_tag="JN334_000527"
     CDS             complement(14723..15097)
                     /locus_tag="JN334_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HAY7436785.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15101..16249)
                     /gene="wzy"
                     /locus_tag="JN334_000528"
     CDS             complement(15101..16249)
                     /gene="wzy"
                     /locus_tag="JN334_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HAY7436786.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16269..17138)
                     /locus_tag="JN334_000529"
     CDS             complement(16269..17138)
                     /locus_tag="JN334_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HAY7436787.1"
                     /translation="MATYNGECWIEEQLKSIIEQKDVDISIFISDDLSTDNTLNICEE
                     FQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQDDIWYKDKIKNAI
                     DCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPGCTYVIKKETLIE
                     FKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQHENNQVGANISFK
                     AKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILALSFWKLRRKKAD
                     KIYILLMLILNIF"
     gene            complement(17167..18057)
                     /locus_tag="JN334_000530"
     CDS             complement(17167..18057)
                     /locus_tag="JN334_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HAY7436788.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18087..19343)
                     /gene="wzx"
                     /locus_tag="JN334_000531"
     CDS             complement(18087..19343)
                     /gene="wzx"
                     /locus_tag="JN334_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HAY7436789.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19359..19904)
                     /gene="rfbC"
                     /locus_tag="JN334_000532"
     CDS             complement(19359..19904)
                     /gene="rfbC"
                     /locus_tag="JN334_000532"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAY7436790.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19909..20787)
                     /gene="rfbA"
                     /locus_tag="JN334_000533"
     CDS             complement(19909..20787)
                     /gene="rfbA"
                     /locus_tag="JN334_000533"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY7436791.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20846..21745)
                     /gene="rfbD"
                     /locus_tag="JN334_000534"
     CDS             complement(20846..21745)
                     /gene="rfbD"
                     /locus_tag="JN334_000534"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAY7436792.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21745..22830)
                     /gene="rfbB"
                     /locus_tag="JN334_000535"
     CDS             complement(21745..22830)
                     /gene="rfbB"
                     /locus_tag="JN334_000535"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY7436793.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVHAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(23203..24096)
                     /gene="galF"
                     /locus_tag="JN334_000536"
     CDS             complement(23203..24096)
                     /gene="galF"
                     /locus_tag="JN334_000536"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HAY7436794.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24271..25665)
                     /gene="wcaM"
                     /locus_tag="JN334_000537"
     CDS             complement(24271..25665)
                     /gene="wcaM"
                     /locus_tag="JN334_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HAY7436795.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25676..26896)
                     /gene="wcaL"
                     /locus_tag="JN334_000538"
     CDS             complement(25676..26896)
                     /gene="wcaL"
                     /locus_tag="JN334_000538"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HAY7436796.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26893..28173)
                     /gene="wcaK"
                     /locus_tag="JN334_000539"
     CDS             complement(26893..28173)
                     /gene="wcaK"
                     /locus_tag="JN334_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HAY7436797.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28353..29831)
                     /gene="wzxC"
                     /locus_tag="JN334_000540"
     CDS             complement(28353..29831)
                     /gene="wzxC"
                     /locus_tag="JN334_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HAY7436798.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29833..31227)
                     /gene="wcaJ"
                     /locus_tag="JN334_000541"
     CDS             complement(29833..31227)
                     /gene="wcaJ"
                     /locus_tag="JN334_000541"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HAY7436799.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31282..32652)
                     /locus_tag="JN334_000542"
     CDS             complement(31282..32652)
                     /locus_tag="JN334_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAY7436800.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPCAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32845..34281)
                     /gene="cpsB"
                     /locus_tag="JN334_000543"
     CDS             complement(32845..34281)
                     /gene="cpsB"
                     /locus_tag="JN334_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HAY7436801.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34284..35507)
                     /gene="wcaI"
                     /locus_tag="JN334_000544"
     CDS             complement(34284..35507)
                     /gene="wcaI"
                     /locus_tag="JN334_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HAY7436802.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35504..35983)
                     /locus_tag="JN334_000545"
     CDS             complement(35504..35983)
                     /locus_tag="JN334_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HAY7436803.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35983..36951)
                     /gene="fcl"
                     /locus_tag="JN334_000546"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35983..36951)
                     /gene="fcl"
                     /locus_tag="JN334_000546"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAY7436804.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36954..38075)
                     /gene="gmd"
                     /locus_tag="JN334_000547"
     CDS             complement(36954..38075)
                     /gene="gmd"
                     /locus_tag="JN334_000547"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAY7436805.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38101..38649)
                     /gene="wcaF"
                     /locus_tag="JN334_000548"
     CDS             complement(38101..38649)
                     /gene="wcaF"
                     /locus_tag="JN334_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HAY7436806.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38665..>38786)
                     /locus_tag="JN334_000549"
     CDS             complement(38665..>38786)
                     /locus_tag="JN334_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyl transferase"
                     /protein_id="HAY7436807.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10355 a         9712 c         8729 g         9990 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgaa ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
      601 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
      661 gcttttttat tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga
      721 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
      781 gcgaattact ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
      841 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg
      901 taatggacgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc
      961 ttaaccgccg cgcccagggt gaagatccgc gctactttac cctgcgtcgt ctggatttcg
     1021 gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct
     1081 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     1141 aaggtatctc ttttaaatcc tgcttactga acggttctgt cgaagttgcg ccgcgtgccg
     1201 ggctggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     1261 tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa
     1321 tggaagaatc taaacagcaa ctgatcgaca agctactgac ccgtattcag ggtgtgatcc
     1381 aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgcctg gatgaagtca
     1441 tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gatcaacagc
     1501 gcgtagcgat gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     1561 aagcactggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     1621 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     1681 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct
     1741 cgataacgtg aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa
     1801 aaccacggtt accgcgctga aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct
     1861 gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa ccttccacac
     1921 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt
     1981 cacgcgcccg gtggcttcag tgggtttgta tattccgggc ggctccgccc cgctcttctc
     2041 aacggtatta atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     2101 ctcaccgccg ccgattgccg atgaaattct ttatgcggcg cagctgtgcg atgtgcagga
     2161 cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt
     2221 gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag caaaacgcca
     2281 ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct
     2341 ggtgattgct gacagcggcg ctacgccgga ttttgtggct tctgatttgc tttctcaggc
     2401 tgaacacggc cctgactcac aggtaatttt actgaccccc gatgctgata tggcgcgtcg
     2461 cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa ccacccgcca
     2521 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tggagatctc
     2581 caaccagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga
     2641 tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggcga
     2701 ctacgcctcc ggcaccaacc acgtgctgcc gacttacggt tacaccgcca cctgttccag
     2761 cctcgggctg gcagatttcc agaaacgcat gaccgtacag gaactgtcga aagaaggttt
     2821 ctccgcgctg gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa
     2881 aaatgccgtt actttgcgtg ttaacgtcct taaggagcaa gcatgagcac cgtgactatt
     2941 actgatttag cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg
     3001 ggcggtaacg gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt ggagtttaag
     3061 cttactcagc aaacgctcaa ccgctacccg gaatgccagc caaaagcggt gattgaaaat
     3121 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     3181 ggtattgaac tgctgatccg cgctttttgc gaaccgggta aagacgccat cctctactgc
     3241 ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca
     3301 gtgccgacgc tggaaaactg gcaactggac ttacagggca tttccgacaa gctggacggc
     3361 gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat caatccgcag
     3421 gattttcgca ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct
     3481 tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata tccgcacctg
     3541 gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg cggatttacg
     3601 ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcccccccta cccgctctcg
     3661 acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc
     3721 gaacgggtgg cgcaaattat tacagaacgc gaatacctga ttgccgcact gaaagagatc
     3781 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     3841 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     3901 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     3961 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     4021 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac
     4081 tcgcctttga accgggcgtg atcccggaat tgctgaagct gcaaaaagcg ggctacaagc
     4141 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcaa gcggattttg
     4201 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     4261 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     4321 tggtggaggg ttatctggct gagcaagcga tggatcgtgc caacagttat gtaattggcg
     4381 atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc
     4441 gcgaaaccct gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc
     4501 atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag
     4561 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     4621 cccacggcgg tttccgcatg gaaatcaatg tcaaaggcga cctctatatc gacgatcacc
     4681 acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca
     4741 aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     4801 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgtg
     4861 tgggcgatct cagcactgaa atgatcgaac acttcttccg ttcgctctct tacaccatgg
     4921 gcgtgacact acacctgaaa accaaaggta aaaacgatca tcaccgtgta gaaagcctgt
     4981 tcaaagcctt tggtcgtacc ctgcgccagg ccatccgcgt cgaaggcgac accctgccct
     5041 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     5101 ctctgtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     5161 cattgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac aagcagcgat
     5221 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     5281 gggcatctgc ttagggatgc aactcctggg acgacgcagc gaagagagca atggcgtcga
     5341 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccactgcc
     5401 acatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc aggggattga
     5461 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
     5521 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
     5581 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
     5641 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
     5701 cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat
     5761 tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc aaaagatccg
     5821 gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag
     5881 gttggcggcg gtgttcgtag tgaagaagac gtggcggcgt tactggaagc tggcgttgcg
     5941 cgcgtggtgg tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa
     6001 cgtttcggtg ccgatgcgct ggtgctggca ctggatgtcc gtattgacga gcaaggcaac
     6061 aagcaagtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
     6121 gaaacctatc tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc
     6181 acgctggcgg gatctaacgt ctctttatat gaagaagtgt gtgccagata tccgcaagtg
     6241 gcgtttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct acgtggcact
     6301 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
     6361 gccatcgcat gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg
     6421 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
     6481 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
     6541 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
     6601 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
     6661 ataaaatttc catcaactcc cctgcgctgg cagacccaac attaattact cgcctggccg
     6721 atcgctttgg cgtgcagtgt attgtggttg gtattgatac ctggtacgac gccgaaaccg
     6781 gtaaatatca tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg
     6841 aaacgctcga ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca
     6901 tgatgaatca ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg
     6961 aagtttgcca cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctgg
     7021 aagccttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc cacaaacaaa
     7081 taatcaatat tggcgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
     7141 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggtc tgatgccggt
     7201 gattgtgcaa cacgcggtat ctggcgaagt gttaatgctg ggctacatga acccggaagc
     7261 cttagacaaa accatcgaaa gcggtaaagt caccttcttc tcgcgcacta aacagcgact
     7321 gtggatcaaa ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg ccccggactg
     7381 cgacaacgac acattactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac
     7441 cagtagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
     7501 gctcgccgaa cgtaaatatg ccgacccgga aacctcctac accgccaaac tgtatgccag
     7561 cggcactaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
     7621 aacggtacat gatcgctttg agctgaccaa cgaggcatct gacttgatgt atcacctgct
     7681 ggtgctgttg caggatcagg atctggattt aacgacagta attgagaacc tgcataaacg
     7741 gcatcagtga gttacgggct gagcaggtgt gatgttgttg tcacacccgg agccattgcc
     7801 cgatgcgcaa acttatcgtg cctacacatt atctcaacac acgtaggccg gataagatgc
     7861 gccagcatcg catccggcaa aaaaaacggg catggtgtca ccaccctgcc cgtttctctt
     7921 aaatgcacaa taatattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat
     7981 ccccgcgcca accatgccgc ccagaagcac tgccagaatc aaggtgattg cctttttcgg
     8041 actatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag
     8101 atcatcaaac tttaattttt caatatccaa caggttttga cgtgtctgat agtagtttgg
     8161 tgagaacacc aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc
     8221 tagaaggaac aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac
     8281 ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg
     8341 ctcctgcgcg accacttcct gggttcttaa agagtcctgc aagtttttcc gtcccagagc
     8401 aatgttgtcc ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat
     8461 gtattgggcc aacttcattt gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa
     8521 tggtaattgc tggttcttaa cagaaggttc gatggtaagt ttttctggct cttcctgatt
     8581 atccagcgtt tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg
     8641 caaatccgat actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc
     8701 agcaatttgc cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc
     8761 tactgccaaa taaccaatag ccagggcaat agccacaatg acggaaatga taattgtcat
     8821 cttgccacgc cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt
     8881 ttgcccagaa acattattat tttctactct catcattacc ctaactgacg gaaatttcca
     8941 agtccgcata gtttagcgat aatccccgtg aatgctatag gataagtgat aaaaataatg
     9001 aattattgcg gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt
     9061 agtcgctgcc aaagagatcg cgggtgtaga ccttatctgc cacatcctta agctcttctg
     9121 ccatacggtt ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca
     9181 ggcgagagtt gaagaatgag tcttctttca tcaccggctc gtagatgatc acttcaacgc
     9241 ctttcgcctt gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgacc
     9301 cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcatcga caattgccga
     9361 gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat ctttcggcag
     9421 acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag
     9481 acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcat aactatccag
     9541 ttcattaaag tatgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc
     9601 tgcttcagtg gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa
     9661 caacgcagca aaacgttctg cgcgttctga acgctcaccg atgacaatac gtgacggatg
     9721 gagattatcg taaagggctt taccttcgcg gaggaattcc ggagagaaaa taatattttc
     9781 agtacgatat ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac
     9841 cataaccgca taaggattta tctcaactac gtctttaatt actgattcta cactggatgt
     9901 attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga cataatcagc
     9961 atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat ctgattgcaa
    10021 aaactgctga atttccttat caacaatagg agatatccga tcattcagca tagcaacgcg
    10081 tgacggtaaa atatctaatg ccacaacctc atgattttgt gcgattagaa gcccgtttga
    10141 caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc agaattaact
    10201 taactgtgaa tcatgatgtt tttagcatcg tgataagatg ctaaaagttt taacgctacg
    10261 ttgtcagatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgatg
    10321 tttattaagt taatattaag cagtgaaatt tagtctataa gatatttggc aaaaaaaagc
    10381 ccggtgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca
    10441 ttcagtatgg aacacacctt ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc
    10501 acgctgtgcc tggatcaggt tcgcaggcag aaccgcggca cggtagctat cgtaataggc
    10561 aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcataag cgacgacatc
    10621 gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggggcca gcagcaggtt
    10681 agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat
    10741 gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc aattgtattc
    10801 ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt tgcccagata
    10861 cagcgcactg cgaacttttt cgatgaactc agccttgtcg cctgctgact gagcttgcgg
    10921 accagagaga actttagatg cggcaacgcg ctgatctttc agagaagaga tataacgtgc
    10981 aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt
    11041 ccatttaccg gtgcctttgt ttgctgcttc atccagaatc acatcaacca ggtagttacc
    11101 gtcttcatct tttttggtga agatatcttt agtgatgtcg atcaggtagc tgctaagttc
    11161 accgttattc cactcggtga aagtctgggc cagttcttca ttagatagat tcaggccacc
    11221 tttgagcaga gaatacgctt cagcaatgag ctgcatatct ccgtattcaa taccattgtg
    11281 aaccatcttc acataatggc ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc
    11341 gtcttctgcc acggcagcga ttttagtcag gataggcgca accagctcat aagcctcttt
    11401 ctgcccgcca ggcataatag aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc
    11461 ggtaccgatg aagttaaagc cctctgctga aagctcacga ttacgacgaa tggtgtcctg
    11521 gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgagatatg gtttcaggga
    11581 atcaatcgca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt
    11641 ttccagagat tcaacaaact ctttcaccgt atagtaagga gccagtttct tgcctggatt
    11701 ttcagcaatc acttcttccg tcttttcgcg ggagcggttg aaaatagaga cggtataacc
    11761 acgactttcg atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat
    11821 ctgttgcttt gacattgttt actcctgtct tctatattat taaccgtcta ttaaccgtta
    11881 tattattaac cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt tacagcatgc
    11941 ttcttacttt atagcgataa gtatgccatt gggtgtatcc ataagaactt tgaattttgg
    12001 atacttttct ggattaaaat tagcatagct tcgtttagat ataacaataa acgaactatt
    12061 aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact
    12121 aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata
    12181 gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata actcactata
    12241 ttcacttgct ttcttcatca gttcatagct atagcgactt tctaagcgtg aattaaaatt
    12301 caagtattca gaataaaaat atttgaattc aaaatacaac gatcgccccg aaaaaaaata
    12361 atacacacaa ggataaaata tcgtcccaac ttgtaatttg taatgttgat atcttttatt
    12421 aaacttgcaa ttgagatcgc aacctgcaat actgacaatg ggtaaatggg tgataaatac
    12481 ctgtcacctt gcgtaacaga aaaccaaaat aagacatatg ttaaaacaat cgagaaaaat
    12541 acccaaagta ttttattatt cctaagtgta aagacagaca gtatagcata aaaaacaaca
    12601 tcactgccaa ctttattaat tgcttgttgc aaatctaatg tggcaggact tataccatgt
    12661 cgagcttgtt ctgctgtttg aacttgtaga tcaatttcat tccataggta gtgaccaaat
    12721 atatttcctc cagcaggact aaaaggatca ccagagatcc aaaaactacg gacataccaa
    12781 taaatgccac caagaattgt tgccaagcta aaaataagaa atgtctgtat gtttctcttt
    12841 ttcgctatca atataacaaa tatgatggct ccatatatgg caccaaatag ttttacgcca
    12901 caagcaattc caataaaaaa accacataaa aaaattaatt cattttgtgt tttattgttt
    12961 tctgagcgaa tatatataag aatactggct gcagcaaaac aaagatagcg ttaaatcaat
    13021 atatgcataa ccaagatgat ctttaagtgg attgatagta aatagtagga ttgtagcaat
    13081 aatgcctgga taataagttt tcgtaatttc ccatgaacag ccaacaagtc cacaggctat
    13141 tacaaaaaaa cagatatttg ctaaaaactg agctaacctt acatctccta actgtaaacc
    13201 taaagccatt aaaagatctg cattttgcgg gaacagagga aaacgcaaat attgttcaac
    13261 aacaattgca tggtgctcaa ctaatgaacg agccaaaggt aagtgataca tagtatcatc
    13321 ccaatcaccg ggagctcgca gggacgataa caatgtaacc acaaaaatca aaacaagaaa
    13381 taaaataaca aacagcgtac tgaactcaat attatttatt gccagccatc taaattgctg
    13441 acgtttaata atgcttaaaa cccaacacac cgtaaaaatt aaaaagatag caatcttatt
    13501 aaattgcccg accatgccca gaaagtaaat tatcaaaata tttaaagaca tccctatgca
    13561 taggcaataa aaaataaaga aaatttgtta agtcctgcca ccccctcctt aaaaaaagat
    13621 gacaatacaa tattaccaat gacaaaaaat gtcattccaa aagcaaaaca atacacaaca
    13681 tacaaataca acggcaattc aatcatagta aaatactcta tttacattat cagtttatag
    13741 tttttttcag aaagataaaa taatgccttc atttcacgtc tagttgttgt atttgaatgc
    13801 aaaataatgc cacatacaaa tgccattatt gacgataaca tgatggaggc agttaaaaag
    13861 gcagtcggaa atcttctcac caaacctgta tcaatataat caacaatgat gggcaatcct
    13921 aaaattatag acaaagcagc aagtaaaacg ccgataatac taaaaaagta tagtggccgt
    13981 tcaagcgagt aaagtttaat tattgttttc agaatcttga acccatcact ccaagttgat
    14041 agtttgctca cactcccttc agatcgctcg ccatattttg tattaacctc accatacttc
    14101 atacgtaatt ctaaagcatg aattgtaagc tcagtttcga tttcaaatcc ccttgataga
    14161 catggaaaag atttaacata gcgtctggaa aatacacgat accccgacaa catatcagta
    14221 aaattccctt taaagatagt ctgaacagta ccagtcaaca atttatttcc tcagcgatgc
    14281 ccttttctgt atgtattttg atctccattc tcttgacgac atcctacaac catatccaat
    14341 tgatttttta tgagatggtt aatcatttct ggagcagaac ttgcatcata agtgtcatct
    14401 ccatcaacca ttagataaat atccgcatct acatctgaga acatacgtct tacgacattc
    14461 cccttgcctt tcagaggaac agagtgaact gttgctccag catcctctgc cacttgtgct
    14521 gtactgtcag tcgaattatt atcaaataca tatatagaag cagttggaat attcgttttg
    14581 aaatcattta tgacttttgc tattgccttc tgctcattat aacaaggaat aattacagcg
    14641 atattaaaag agtgatacat aagcccctca tataaactat ttagctaatg tgcaagtaat
    14701 tgtgttttta acaaatcgtt tactaacgaa aaatgaaata ttttgatgcg caatatccta
    14761 aaaccaaact aaaaagagaa aaggtgaaca atgtaaatat tggagataaa gctaagagat
    14821 caaataaaac gccgaataaa taactcatcg cccccataaa aaatataaat ataaaatatc
    14881 tagccttggt tgcagatact ttaaatgagc atcttgcatt tgcaaaaaaa gaaaaggttg
    14941 ctgcacatat aaaaccaaca atgttacttc ggccttgacc aaaagctaaa ttattataac
    15001 acgcataaaa taccccccaa tgtaaagcag tattgacaat tccaattaaa aaataactaa
    15061 aaaaagaact ggtcaataac ttccctattt ttaacatcct ttattttgct ccagaagtga
    15121 ggttattact aatttggata ttttctatag aaaataccca taaaactaca acgcctataa
    15181 taaaaattcc catgggtgta aaaagaaatg gattagttat agaaacggta atactgaaaa
    15241 aaagtattaa cgacatcatt aatgatgttt ttgtactatt taaaaaatag cctttcaaaa
    15301 agataagcaa cataggaact aatgatataa atacaccaat aacgcctgtt ttctgaagta
    15361 tctcaagagg tactatttca atccttaatc gatctaatat aaatgaacca aatcctcttc
    15421 caaacaagaa cgtcgctaaa tcaacatttt tataataaaa atataaatca ttaaatctaa
    15481 cagagtcaga atcttcactg cgtgatccga ccatgtaaag cataattata aatagaacgc
    15541 tgcccaatat tattataata gtatttaaat atagcttatg taatttaaaa ctggctaata
    15601 acagtgataa tgttgtaaaa agtaatacac ctcttgattc tgtcaataat atcgcaagca
    15661 ctattatgag acattttaaa tattttttgt cgacaaaact gataatcgca ccgacaccaa
    15721 aaaagtagaa ggatttggaa aaaaaagccc catcgggacg aaaaaagaac tcgctagtca
    15781 atgataagtg ttcataaatt aaattaaaat ttaacaaacc gaaattcaac aaaattatgt
    15841 atgaaatata taacagtgat ccatataatg caaatgcatt aactatttta ataaaaatct
    15901 cacatgaaaa tacttttaaa gtatatctta gtgtaaatag atatataagt gacagcataa
    15961 aaaggtatgg ttgcacatca aacagtatat catttatttt attaccagct aataagccga
    16021 caaagaagcc ataaaaagga aataaaagca accatataaa aagactgaca catttttggg
    16081 tgattgaaaa attgaatgta actaaattaa aaattaaaag tataaaacta aatacaaata
    16141 ataaatatcg caaagggaat attcccaatg gctccagtaa tcttccacca ccaccaataa
    16201 tcagttcaat acataaaaat gttataaaaa ttttatttat attattcata ttcgtaaggt
    16261 gatgtttttt aaaatatgtt taaaatcaac attaataaaa tataaatttt atcagccttt
    16321 ttccgcctta acttccaaaa acttaaagcc aagatcaaat tgcctatata acctttaccc
    16381 aattgattat tcacaaagct atcatcagct aaggcattag ctattttagt tacttctttt
    16441 cgataccact tgtttctcac caaaccaagc cgtttgtatt ttgctttaaa actaatgttt
    16501 gctcccactt ggttgttttc atgctgtctg tacagcattg ttggttttct atcaatatac
    16561 cacgaataat ttctcgtccg cgcaaatgaa tataaaaacc aatcatgcag acagatatct
    16621 tgtgcagcat ttttattatt tatgatgaac ttcttaaatt caataagcgt ttcttttttt
    16681 ataacatatg tacaccccgg acctgctgct tcaaaaaaat aatcgaattg agtttgcgac
    16741 tgagccttat caactaaatt ttttcgccct gatggataat atgcaattac attgctggaa
    16801 taacaattcg cattattaaa taccaagcag tcaattgcat tttttatttt atctttatac
    16861 caaatatcgt cctgatcact aaaacagata taatcatagt tttcaagatc gacatcttta
    16921 attaatctat aaaaattttt acctgccccc ccaaacttat tgacgcttgg aagtatattt
    16981 ataattgagg gatatgataa ttgaaattct tcacaaatat ttaatgtatt atcagttgat
    17041 aaatcatctg atataaaaat tgagatgtct acatcttttt gttcaataat actttttaat
    17101 tgttcctcaa tccagcattc gccattatac gtagccataa gtacagctac tcttttttga
    17161 tcagaactat gcattataac gaccgccccc agtaattcct cttattccat ccagcgtagc
    17221 caaaatatgt tttttcatat attcaatttt atttttttga gttgcgataa ttaaagtcgt
    17281 atgaattaaa gaaaaaacaa cttccctaat agagagtaaa agaggcacat gattttttct
    17341 aagcaaaaga aatgcatttc ttactcgata ataacgacgt actggcgagt gaattggtat
    17401 gttaatccca cagattttta ctgacttatc cccaatagca tgaaccattt ctatatcagg
    17461 aatgatatga actaatatgc catagtttaa gcatctaaga caccattctg tatcgacata
    17521 atcaataaaa agttcctcat ccatcattcc aacaatctca aaaacttctt ttgaaaccat
    17581 ggttccggat gagattgtta ctgaactttt aaacggtgtc tgtccttcag tgacatgaat
    17641 tttctctcga agaccatttt ttttgatatt acaaatagga taataatgtg acttatctct
    17701 atcaataaaa actgggccag ctatttttgc attttcatta tccatatagc aggaaagttt
    17761 tttgataaac tcattgcgaa tacggctatc ttgatcaaaa aaaattatct tctctgcccc
    17821 tttttcaaga gaatatcgaa tcccaatatt ttgtgctttt gctatacctt cattcctacc
    17881 aagacaaatt aaatgaactt tttcaatgtc tgctaattta tctttcaagg tgaaattagt
    17941 cctattatcg actactacaa cgtaacaatt ttgctccttt agttcagtga tcagtgcatt
    18001 cagattttta agttcgggat tatatgtcac aatgacagcg taaatattac tattcattat
    18061 ccaagtgact cagtaattgg ttaattttag ttttcatata cagaacacat tctagtataa
    18121 agccattgta ataacagagc atctaaaatt acacgtatgc tccaggatat tgcggcaccc
    18181 agtaacccat atttttcgac cataaaatac aaaagggcta aataaggaac caattcagca
    18241 caatgaatca aagcagtaat tttagatttt cctaatgcct ggattgcaga gaatggtatc
    18301 attgccagcg cattgaagaa aaaaccgatc aataacacat ttaatatatt aaaggaatta
    18361 atttgataat tctcaccaaa ccatattttc aatacaagcc ctgaatataa caagcctatg
    18421 attatcaccg gcaaacagat tagaaacatt aaaagcaatg atttatttac attacgttta
    18481 aattctttaa agtctttgat gttacttaac cttggaaaca ctgccctccc gattgctgca
    18541 ggtattattc ctaattttag aataacctcc gctggtgcag aataaaatgc aactttatct
    18601 gcccccataa tatttgacac tataaatcga tcaaaataca ccatgaccgg acttatgata
    18661 ttacttactg tcatccaacc gccaaagaaa aagagtcgtt taaaggtttt ataacaaaaa
    18721 tgaacccccg aaattttaat atcattcctg acataatagg cagagactaa aatcgaaata
    18781 actcttgcaa atattaaacc agcaaccgct gccgacaatg tagcactata aaaaacaaat
    18841 atggccggaa ttcccgcgat gcaagagctt gatatagatt tttgaatatt tacaatgcca
    18901 aatttttcat ccccctcaag aatggctgac cataattgat ttagaataaa taatggaatg
    18961 caaatagcca acagtttaaa tgctagttgt atatcactat gttcaacacc agaaattttt
    19021 agataattaa caattccatc agagaaaatc aataataaaa aagcaccaaa gcatgaaaat
    19081 agcaccaaaa aagatgtact tgttgaaatt accttggttc tttcatgatg attatcacga
    19141 tgaattgcaa tttctctaat aacagagcga gtcaggccta catcaaaaat tccagcatac
    19201 ccaacaagtg caattgcaat tgtataaaca ccgaaacctt caggccccaa acctcgagcg
    19261 agaaatccta gcgaggggat agcaattaaa gttggtattg catagccaaa aaggttccag
    19321 acactatttt ttattatgct cattattaca tttctttatc atataaaata atcagcatct
    19381 acaaattttt gaccattaat atctttatca gaaagcaatg gattttgaac cggccattct
    19441 atatttatac ttttgtcgct ccataaaatc ccacgctctt gcatttttga atagaaatta
    19501 tttgttttgt agacaaattc tgcttcatca ctaagaacgc aaaacccatg agcgaatcct
    19561 tccggtatcc ataattgacg cttattctcc gccgacaaat ttacaccaac ccacttgcca
    19621 aatgttggtg atgatttccg aatatcaaca gctacgtcaa aaacttcacc gacaacacag
    19681 cgtaccagtt ttccttgagc ataaggttcc agctgatagt gcagtccgcg taacacaccc
    19741 ttagttgatt tagaatggtt atcctgaaca aactcaacct ttcgtcctac agcctcttcg
    19801 aaaactttct gattgaagct ttccataaaa aagccccgct catcaccaaa tacttttggt
    19861 tcaaaaatta gcacatcagg aatttcagtt ttaattacgt tcattttatt aataaccttt
    19921 aatcattttc agcagatact gaccataagc atttttcttc agcggctcgg ctaatacctt
    19981 cacttgctca gcatcaataa agcctttacg gtgagcaatt tcttccgggc aggaaacttt
    20041 cagcccctga cgctcttcaa ttgttgcaat gaagttgctt gcttcaataa gactttgatg
    20101 tgtccccgtg tccagccatg cataaccacg ccccatcata gcgacagaca aacgtccttg
    20161 gtccatataa atacggttaa tatcggtaat ttccagttcg ccacgtgcag atggtttaag
    20221 gtttttcgcc atttctacaa cgtcattgtc atagaaataa agcccagtaa ccgcatagtt
    20281 actttttggt tcctgcggtt tttcttccag actaattgcc gtaccgttat catcaaattc
    20341 caccacaccg tagcgctcag gatcatttac gtgataggca aaaaccgttg cgccactctc
    20401 tctgttaaca gcggtatcca ttaatttcgg caggtcgtga ccatagaaga tattatcacc
    20461 aagtaccagc gcacaatcat caccacctat aaattcttca ccaatgataa atgcttgcgc
    20521 cagaccatcc ggactcggtt gtactttata ttgcagatta agcccccact ggcttccatc
    20581 accaagcaat tgttgaaaac gtggtgtatc ctgcggcgta ctgataatca gaatatctcg
    20641 aatacccgcc aacataagcg ttgaaagcgg ataataaatc atcggtttat cataaatcgg
    20701 tagcagttgt ttgctcacag ccattgtcac aggataaaga cgagtgccgg aaccaccagc
    20761 cagaataata cctttacgcg ttttcatttc accattcctt ttaattcatc tcgctctagc
    20821 atcatgagcg agatgcaata atttgttaaa ttgccgtggt cgtaaataat tcgttgagca
    20881 tacgtttcac gcccacctgc cagtcaggca agacaagcgc aaagttctgc tgaaattttt
    20941 cggtattgag gcgagaatta tggggacgac gtgctggtgt gggataggcc gttgtcggca
    21001 cggcattaag tttgttaaga gcaaggttga tccccgctct gcgcgcttct tcaaatacca
    21061 gagcggcata atcgtgccag gttgttgtac caccagcaac cagatggtac aaaccagcaa
    21121 cttctggttt atttgcggcc acgcgaatgg catgagcggt gcaatcagcc agtaattcag
    21181 cccccgttgg tgcgccgaat tgatcgttta ttacagccaa ctcttcgcgt tcttttgcca
    21241 ggcgcaacat cgttttggca aagttatttc ctttaccagc gtatacccag ctggtacgga
    21301 aaataagatg ctttgcgcaa tgttcttgta acgctttttc cccagctagc ttggtttcac
    21361 cgtagacatt tagcggtgct gttgcatccg tttccagcca tggcgtgtca ccgttgcctg
    21421 ggaaaacata atcagtggag tagtgtataa cccaggctcc gacttcatta gctgcttttg
    21481 caatcgcttc gacgcatgtc gcgttaagta attgcgcgaa attcggttct gattccgctt
    21541 tgtctactgc agtgtgagca gcagcattaa caataacatc ggggcgaatt tttttgacgg
    21601 tttcagccac accttcgggg ttgctgaaat ctccacaata atcagtggag tgaacatcga
    21661 gcgcaatcaa gttacccaga ggagccagag cacgctgcag ttcccaaccc acctgccctg
    21721 ttttgccgaa aagcaggata ttcattactg acggccctca tagttttgtt caatccacga
    21781 ctgataagta ccgcttttca cattctcaac ccaatttgta ttagccagat accattccac
    21841 cgttttacga atcccgctct caaacgtttc ttgcggtttc cagcccaatt cgcggctaat
    21901 tttatcggca tcaatggcgt aacggcggtc atgccccggg cgatcggcaa cataagtaat
    21961 ttgctcacga taagatttct ctttcggtac aatctcatcc aacaaatcac aaatagtgag
    22021 cactacgtcg atgtttttct tttcgttatg tccaccaatg ttataagttt cacccgcttt
    22081 accttcggtt acgacggtat ataacgcacg agcatgatcc tctacataca accagtcgcg
    22141 aatttgatcc cctttaccat aaataggtaa tgccttacct tccagagcat tcagaataac
    22201 caatggaata agtttttccg ggaagtgata aggaccataa ttgttcgagc aattagtcac
    22261 aatggttggt aaaccataag tccgcttcca ggcatgtacc aaatgatcgc tggaagcttt
    22321 agacgcagaa tacgggctac ttggcgcgta agctgtcgtt tccgtaaata gcggtaacgc
    22381 ttcgttatta tttgcttcat ccggatgggg taagtcacca taaacttcat cagtagaaat
    22441 atgatgaaag cggaaacttt ttttcttttc atcattcaga gcagaccagt aattgcgcgc
    22501 cgcttctaaa agaacataag tccccacaat attggtttca ataaatgccg cagggcccgt
    22561 tattgaacgg tcaacgtggc tctctgccgc caggtgcatc accgcgtctg gctggtgctg
    22621 tgcgaaaata cgcgacatgg ctacagcatc gcagatatct gcatgctcaa aagcataacg
    22681 ttcagaatca gaaacatcag cgagtgattc caggttcccg gcgtatgtta atttatcgac
    22741 attaacaaca ctatcttgcg tattatttat tatgtgacga acaacagcag aaccaataaa
    22801 ccctgcgcca ccagtaacaa gtatcttcac ttttttattc catatagcta tagagcatgc
    22861 tgtgaaatag actgctccag atttgattaa tagaggtatt aatacacgct accgcccctg
    22921 gcttaacagc taccagtgca ctgcatgcat ctcaacgatg tggcgagcgt tagccactcg
    22981 cgccaaatcc gaaaaattca aacgttaatt gtcttaccaa acagccgtag aaacaaggaa
    23041 aatcctggaa aaatttgaat aatatgagct atgtaacttc ttgtttttat aggctttata
    23101 aaaacggcaa cggcaggaaa attcaatgag caaaacttat gccacgaatc ttcactgccg
    23161 ttataatttt cttatcaacc gttacatccg gtcagatttt cattattcgc ttaacagctt
    23221 ctcaatacct ttacggaact tcgccccttc tttcaggttg cgtaggccat acttcacaaa
    23281 cgcctgcata tagcccattt ttttgccgca gtcgtaactg tcgccggtca tcagcattgc
    23341 atcaacggat tgttttttcg ccagctcggc aatagcatca gtcagctgaa tacgtcccca
    23401 tgcaccaggc tgagtacgtt ccagttccgg ccaaatatcg gcagaaagca catagcgacc
    23461 tacggccatg atgtctgagt ccagcgtctg cggctgatcc ggtttttcga taaattcaac
    23521 aatgcggctg actttaccct cacggtccag cggctcttta gtctggatga cggagtattc
    23581 agagaggtca cccggcatac gttttgccag cacctggctg cggcccgttt cgttgaaacg
    23641 tgcaatcatg gcagcaaggt tgtaacgtag cgggtcggcg ctggcatcgt cgatcacaac
    23701 gtctggcagt accacgacaa atgggttgtc accaatggca ggtcgcgcac acaaaatgga
    23761 gtggcctaaa cctaaaggtt cgccctgacg cacgttcata atgatcacgc ccggcggaca
    23821 gatggactgt acttccgcca gcagttgacg cttcacgcgc tgctcaagga gtgattctaa
    23881 ctcataagag gtgtcgaagt ggttttcgac cgcgttcttg gacgcgtgag ttaccaggag
    23941 gatttctttg atccctgcag ccacaatctc gtcaacaatg tactgaatca ttggcttgtc
    24001 gacgattggt agcatctctt tgggtatcgc cttagtggca ggcaacatat gcatcccgag
    24061 acccgctaca ggaataactg cttttaaatt cgtcattatt tcatccacct gtaaaatggt
    24121 tgctgaatta tagcttgttc gatttttttc gccagcatca attaccctga attgattact
    24181 gaattacttg tgatgttacg ccgcttcgtt gtggattgca gtagcattgt tcctaagtat
    24241 ggctccattt ttccaggaat ggtcgcaaat ctactccctc cgttccggca atctaaagtt
    24301 aatcttttcc acattaacaa tatggtgatt aatcctgtcg atatcgacgg agctttgccc
    24361 tctttcattc accgcatgaa catttgcaag agacagcagt gtttcttttt tcgccataaa
    24421 gacgccgcga acgtctttgc gcatgtcgaa gttcatgctc aatgctggtc caactgagga
    24481 ttcctgcatc acattgatat ttcgcataaa aagatgttgc ggcttgttgt gtaactccag
    24541 cgaggcacgc ttcatctcaa tgttagttag cgacacaaag gagacggcat tcccggcgga
    24601 gatttggatg ccgcgcaatt tataagcaag atgggtatta tccagttgaa tattattcac
    24661 tcggaaattt tgcggtatcg agagatattt gcctttaatt accccatagc cgattaacat
    24721 cccggcgcta ttaatcattt caatattatc aatcacgaaa ttgtcacaac cgtaaatagc
    24781 gactgtcgcg ttatcaatgc ccgctttctt actgaaatcc ggcgtgatat tgcgggcatt
    24841 gatattacga ataacaaaat gtttaccatt ttcaacatgg atcaactgcc gacaatccga
    24901 tcccgtgata ttcgccacga caaagttttt cactgcctgg tcttccgggt agttgttatc
    24961 ataagtgctt cccgcaaggc ctatgccgat gccccagttg attttgccgt tggtacagtt
    25021 gatacgctcg atgacatggt cagatatcaa aatatcactg tcgttaattg ccacgttcca
    25081 ttcaatggcg tcgccctgta agtcgctgaa cttacagttg gtaatgttgg caccgataat
    25141 ctggttatga aatccctggc gtaagatggc gtaattagcg tggctgacag tgaggttatc
    25201 gatggtcagg ttgcgcatga cccgtttgtt tttgccgcca atataaatct gcattaccgg
    25261 gccaaagccg ctcatcgcca gccctttgat ggtgcagtca gaaccacgca catccagggt
    25321 aatgttatgc ataccgccgc cctctccccc tgtcacctgg ctgccgtcct gtaagacaaa
    25381 tcgccctctg ccgttgccgc gcaggcttcc aaggatgtgt aacgttttgc cgggagggat
    25441 gaagatgccg gtgttgatat tgtcacaaac gaatccggca ggcacgacga ccgtttgccc
    25501 ttcgctgaag gcttgtttaa atgaggcgat ccagtcatgt gggttgtagt cgttaatgtt
    25561 aacgctttgt cgggcgggaa gtgcgcgagc gaaaggggta tggaggaagg caagcgccga
    25621 gcttgccgtc aggaaggtgc gtcgggagag tgttttaaat ggcatacgtt ctcctctata
    25681 aagcctgcag caagctggcg agttctcgat taatcacctg ctggttaaaa tcgtgttcga
    25741 ctttttcgca cgcacgtttg acgaccggag ccagttcgtc ggtatccagt tggctaaacg
    25801 ccgccagacg ttgtgccagt gcgcgagcat cgttctcagg cagcagccag ccggatttat
    25861 cggcctccac cagttcagga attccgctat gcagagtaga aaccaccgga ataccgaccg
    25921 ccatcgcttc cattagcgcc accggaatgc cttccatatc gccatccgcg cccgttaccg
    25981 atggcaacag gaagacatcc gcgtcgtcga gcatctcctt cacttcatgg ctcggtttaa
    26041 agcccggcat ctctaccaca tcttccagtt gatattgttc gatgatggtg cgcaggcgtc
    26101 gttcccacag gccaatgccg aggatgcgat agcgaaatgc cacgccctgc tctttcaact
    26161 gacggcaggc ttcaatcgcc acatgcaggc cttttttctc ggttaagcgt gcgacagata
    26221 tgatttccag cggcgttgcg ggcgctttca ccggacgcgg gctaaagcgc gtcatgtcca
    26281 cgcccatacg cgatacggcg attttttctc tggggcagcc cattttttgc agccttccgg
    26341 cccacagatc gcttatcggt aacatcaggt cgccacggcg aaacagttgt tgatattcgg
    26401 gagtgtagtg gttgagcact tcccgactgg agatatcaat accgtggaag atggtggcaa
    26461 ttttgccgcg aatgataccc agttcgcgta gttttgctgc ggttaccccc gccgggccaa
    26521 aatgagcgat gaacacatcg gcgcgaaacg gtgttgcgac ctgaccacaa atggcagaca
    26581 aaatcaggtt ccgcgactcg gcaccatagc gtttgaggtt gagcgcatgc caggtatttt
    26641 tacgatgaat gccacgtaag gtctggctgg cgcggtggcg cagtttcgct actttgcccg
    26701 caggttcgtc ctgtaaccag cgggttctgg cgacaaggtt gtatttcgtc catgccgcgt
    26761 gggtgttctc tgtatcgcct ttttgcaaag cgacaatctc cacttcaaag cccatatcaa
    26821 taaacgcggt aatttgattg aggacgaagg tttccgacga cagcgggaat ttcaataaga
    26881 agaagccgac cttcatttca cctccccgat gcgctcaagc acagactgca ccatctgcat
    26941 tcccgtctga cgttcgcgac tgacggcttc gttaaatcga gcgttcagca ccggaagctg
    27001 gcctaaggta tccgcaacca tcgcttgcag gctgccgtcc agtaaatgac gaatatcaat
    27061 cgccatctct ggcagtccca gctgctgcat aatcccggcg gatttatgtt catagttgat
    27121 ggcaatcgcc ggagtagcaa agttcattga gataatggcg gagtgcaggc gcgtaccgac
    27181 ggtgagttca caggctccga gaattttgcc catttccaga tcgttgagtt catccatcac
    27241 tacgtggtaa cgggcaggat cgctgatgtg ctggcgcaga ttgagcgcca ccatgcggtc
    27301 gtctttgtta tagctgtcga tacccgtaca ggtggagagc gcaatcacct gatagccttc
    27361 atcgagaatg cgattgacca ccccggcaaa ggctttttca tacgcttgtt gagtggtgcc
    27421 gagacgtttg tcgaacggtg ccagttcgcg cagggtaatt gccactgttt tctgttgtgc
    27481 ggcaacgtcc agccagtgtt gaacggcata gctggcggtg aagtcttcgg tgtggtgatc
    27541 gaccagccac gcggtatcga cgccatgttc cacttttgcg gtggtgatat ttctgcgttt
    27601 catcaaatca aggctgaccg attcgcgcag gatcagcgcg tcgcagtgac caaagacata
    27661 gttcgccagt tggttaaatt gctcatcctg gaaggggccg acgctgtgac caatcataaa
    27721 cagcggcttt ttcgccataa atgtgcaaag cgcatgttca aactgcggca cgccgtagag
    27781 atcgacaaaa aacgatccgc cgacctgaat aatggcgtcg taacctgaca gcaggcgcac
    27841 gaagtcggtg aatccctggg cgatggcgat attgcgcagc ttgccagtgt cggtgacgcg
    27901 tgagagcaat acctgatgct ggtagcggcg acggaggact tttttaatgc gcccgacaac
    27961 gcccgccgcg ctattgtgtt gtgtcatctg caggaacagc ggatcgccca ttaccgggcg
    28021 gttgagcagc caggaagaac tgaccggata gcggctcatc acgtccactt cggtatgtgg
    28081 attgaggatg ttgatggcat caagtaagcc gcgcaagatg gcgctgtcgc cacgattgcc
    28141 gcaagtgtgg ttgcccagaa taagtaattt cattttttcc tcataaattt gatgccaggt
    28201 gaggctgtgt ttgttgtgtt cgttgccgtt ttttgcctga tgcgacgctg gcgcgtctta
    28261 tcaggcctac aggtcccgag cacagaaccg taggacggat aaggcgtttt acgccgcatc
    28321 cggcaaccgt tgtcggaacc gaaaacagca attcaccccg cccgtaaaag cattttcatt
    28381 ttttcgctgc gacaaaactg acgcttcact tccaccacca gcggatggcg tgacagcaca
    28441 atcatcacca caaacgccag cacgcccgcg gctatttgca ccgccagcag catccccagc
    28501 gccagttgcc ctttcagcac aatgcccagc gcataactga ccaccagcgt cggcaacgag
    28561 agataaaacg gcagccacaa actcaggatg tactggcgat aactggaacc aagaaccggt
    28621 ttaatcatca cgaagtaact cagaatggtg ttgataatct gcaccagcag gaaaccgagc
    28681 gtgacgccga ttgcgcccgc catctgccca ccaataacaa tcgccggaat aaacagaaat
    28741 gttttgaata cgttgaattt aaagctgata tcgacccgcg ctttcgccat cagcagcgaa
    28801 ccaatcgggt tacccacgga gcgcagcaga cccacaatac acagcaattg cagcaccgga
    28861 ataatgctgt tccacttttc gccgaatacc agcggtacga agttattcga caccaccatc
    28921 agccccagca gcgccggaaa gttgataatc cccactatcg acagcagctt gtagaagtta
    28981 acacgcagct tttcggtgtc gtcctgaatt ttggcaaacg ccggaaacaa cacgcgggtg
    29041 atgattgggt tcagcttcat cggcggtacg acggccacgt tgtacgccag gttgtatccc
    29101 cctgccacgc ccgcgccaag aatacgcgcc agcaccagcg ttgaaaggtt ggtgttgaga
    29161 tagttgatga tgctgtccgc cgtcagccag gcaccaaaac gtaagttcgg tgccacagac
    29221 gccagcgaga agtgcagacc ggggcaataa attttgcgtc caaagtagcc aaacagcagt
    29281 gttctcaccg cactattgac cagataaccg aggatcgcgg tcatcgccag cggccagaaa
    29341 tgggcgctaa ctaccgtaaa agtgaagccc gccagcaccg cgctggtttc gatcatgccg
    29401 attttgttga actccagctc cttctgcatc aacgcgcgga actgttgccc gtgggggatc
    29461 accacaaacg ccagcgacaa tgttttaatc aacggtgcca ggtccgggtt attcagcacg
    29521 tcgccgatga catcactcaa caaaaacacc gccacacata ccacaagccc cagcccgacg
    29581 ttcagccagt acagcgtggt gagttcaagg tgactgattt cttttcgctg aataatcgag
    29641 ttagcgatac cgaagtcaga aagcgtatct gccagcgcga taatcaccag cgacacagtc
    29701 agcaggccga actggtggtt gtcgataatc cgcgccagca cggtcatctg caccagcccg
    29761 aggccgatga tgatcaccgt ggcaatagcc gaccacttcg cgccgctgat ggttttttca
    29821 cgtaagctca tatcaatatg ccgctttgtt aacgaagccc ttgaataccg tcaggaaaac
    29881 gattttgata tcgaaccaga cgctccattc gcggatgtac tcaaggtcga actcgacgcg
    29941 tttttccatt ttctccagcg tgtcggtttc gccgcgccag ccattaatct gcgcccagcc
    30001 ggtaatgccc ggtttcactt tatggcgcag catgtagcct tcaatgagct ggcgatactg
    30061 ttcgttatgc gctactgcgt gcggacgtgg accgacaatc gacatccccc cggtcagcac
    30121 attgataaac tgcggcaatt cgtccagcga ggtgcggcgc agaaagttcc ccactttagt
    30181 gacgcgcgga tcgttctgcg tcgcctgggt caccactttg tcgttctcca tcactttcat
    30241 ggaacggaac ttccacactt tgatcggctt gccatccata ccatagcgag tctggcggaa
    30301 aataaccggc cctggtgaac tgagtttcac cgccagcgca atacagcaca gcaccgggga
    30361 gatcagcagc agaataagcg tcgccagcac aatgtcttcc gcacgtttta gcaggcggtt
    30421 aacccccgaa agcggcgtgt catacagcgg caccaccgga acgccgttca tctcttcgag
    30481 acgtgaatgg agaatgttga aggtaaagac gtcggggatc agcagcaccg aacaggtggt
    30541 atccgccagt tgatggacca gttttttcac tcgtgcgccg tcgcacattt gcatcgcgat
    30601 atagacgtta tgaatcttgc ccgcttttgc atcctcgacc agctgttgca ggttacccgc
    30661 ccagtcgtta gaaacgccgc ccggtttcgg gtcgtggtaa acgcccacca cttcaaaccc
    30721 taaccacggc tgattacgga aactctccat cagcatttgt ccggcggcta aatcccccgc
    30781 tacggcgacc atccgcttgt tgtagccatg attacgcagc cagcccgccc caatgcgaat
    30841 acatgaacgg caaaccacca gtccgatgct ggtcagccca taccacgcca gccagatttt
    30901 cagttgcgtg tcgaaatcat tgttgaacgc caccagtccg gcgctgaaaa tcacgcttaa
    30961 ggtccagttt tgtagcaaca gggcaaattc tgtcgctgcc cgaacaccgc gccatgagcg
    31021 ataaaaatcg gtgatgccgc ccagcatctg gaacaccacc agcgtaatca gcgccaccaa
    31081 caggtgcatg tagaggaatg acagtccgct gacttcgcaa accagccata gcccggcaaa
    31141 catgatggtg atatctgaaa agcgttgcac catagagatt aacgatgcat tggttttcgc
    31201 tcgctcgcgc ttttttagat ttgtcatcgt tgttcctgtt attagcccct tacccgcagt
    31261 ggggtaaggg aagatccgac attactcgtt cagcaacgtc agcagagttc gcgttcgcgc
    31321 ttccatcagc ggcacatcac cgcgcgattc cacattcagg cgcaccaccg gttcggtatt
    31381 ggaggtgcgc aggttaaagc gccagtcggc aaaggtcatg ctgatgccat cgctgcgatc
    31441 caccgccagc gcctcacggc taaaatgctg ttccacgcgg ttaatcgcct caacgggttg
    31501 cgccagtttg ctgttgatct caccgcttgc cggaaacgcc gccatccggt cgcgcaccag
    31561 ttcgcccagc gtttttcctt tcaggcacac cagttcggcg accagcagcc acgggatcat
    31621 gccgctgtcg cagtaagcga aatcacggaa gtaatggtgg gcgctcattt cgccaccgta
    31681 gatggcgtct tccttgcgca tccgttcttt aataaaggcg tgtccggttt tcgacattac
    31741 cggcgtgccg cctgcagcag tcaccacatc aatagtgttc caagagagac gcggatcatg
    31801 gatgatcttc gcgcagggat ttttttcgag gaacgcttct gccagcaggc cgacaatgta
    31861 gtagccctcg ataaactgcc ctttttcgtc aaacagaaaa cagcggtcaa aatcgccatc
    31921 aaaggcaatg cccatatccg cgccgtgttt gatgaccgca ttgcgggtgt cgtcgcgaca
    31981 ttccggcagc agcgggttag gaataccgtt ggggaaattg ccgtccggtg tgttgtgtac
    32041 tttgattaat tccacgggcg cgccgagggc tttaaagcgg gcttcaatgg cgtccaccac
    32101 cggacccgct gcgccattcc cggagttgat caccagcttg agcggcgtga gatttttgac
    32161 attgatataa ccgaacaggt gatcaacgta agcgtcacgc aggttgattt gctgatagcg
    32221 accgcgtttg gtttcatcga cgggaggaaa gtcgttggct tcagccagac gctggacgtc
    32281 gcgcagtccg gtatcaccgc tgatcgtgcg agccccctcg cgcaccagct tcatgccgtt
    32341 gtaatccatc ggattatggc tggcagtaac ttcaatgccg ccatccacgc cgagatggaa
    32401 cgtggcgaaa tagatctctt cggtgccgga cataccgata tccagcacat cgacgcccgc
    32461 atcctgtaaa ccttttgcca gcgccagttt taaggtttcg ctggtgagac ggacatcacc
    32521 gcctaacaca atggttttcg gtttgagaaa ttcgccatag gcgcgaccaa tgcgccaggc
    32581 gatatcttca ttcagttctt cgcctaattt cccgcgaata tcataggctt taaagcaggt
    32641 taattttttc atgtcgttac cctttttcag gcaatagttg gccctgaccg aaacggccaa
    32701 ttttattttt gtcgttattt acaccgcggt ttcgcattca ttgcctgatg cgacgctgcc
    32761 gcgtcttatc aggcctacaa aaccgaaccg taggtcggat aaggcgttca cgccgcatcc
    32821 gacaaacagc gcctgatgcg acgtttacac ccgtccgtag cgatccgcga agcgcaccac
    32881 atcatcctct tcgagataag agccggagcg cacttcaatt aaatcgagcg gaattttccc
    32941 cgggttttcc aggcagtgcg tcgcccccag cggaatataa atggactcgt tttcaccaag
    33001 cagtttgata tcaccatcaa tggtgacttt tgccgttccc gcgaccacta cccagtgttc
    33061 cgcgcggtga tggtgcatct gtaccgacaa accctcgccc ggtttcacgg tgatgcgttt
    33121 cacctggtag cggtcgcccg cgtcgataga gtcatatttg ccccacggac gatacacttc
    33181 gcgatgcacc cgatgctcat ggcgaccatc ggctttgatc tgctcgacca cttttttcac
    33241 atcctgtacc gcgttacggt cggcaatcag caccgcatct ttggtctgca ccactaccag
    33301 atctttcacc ccgacggtgg tgaccaggcc tgattcggca tacacatagc tgttttcagt
    33361 tttgtgatta atcacatcgc cgtggcaaac gttgccctcg gcggtgtggg cgctgatctc
    33421 ccataatgaa gaccaggaac caacatcgct ccagcccgca tccatcggca ccacaacagc
    33481 atctgccgta ggttccatga ccgcgtaatc caccgactct tccgggcagg cgagaaacgc
    33541 ttcttcatcc acacgaataa aatcgagatc cggatcgacg gcgctcatcg ctttttcgca
    33601 ggcatcgagg atatccgggc gatatttttt cagttcttcg agatagcgtc cggcgcggaa
    33661 caggaacatg ccgctgttcc agtaatattc gccgcttgct acataggcct gggcggtttc
    33721 cagattcggt ttttcgacaa actgcgccac ttcaaaggcc accgtatcct gctcacccac
    33781 agacacttca ccgcgacgaa tatagccata accggtttct ggtagatccg gcacaatgcc
    33841 aaaggtcacc agcttgcccg cttcggcata aggcatggca ttacgcacgg cggcacggaa
    33901 cgcgtcttca tcggcaatca catgatccgc cgccaatacc agcattaacg ggtcgctctc
    33961 cgggctatga cgttttgccg ccagcgccgc cagtgcaatg gcaggtgcag tgttacgccc
    34021 tgccggttcg agaataatgt tctcggtgag tttgttcagt tgacgcagct gttccgcgac
    34081 aataaagcgg tgctgctcat tgcaaatcac caccgggctt tcgcactcca cgccgttcag
    34141 gcggcagatg gtggtttgca gcatggtgag atcgcctttc aggcataaaa actgcttggg
    34201 gtaaagtacg cgggaaagcg gccataaacg gctaccggag ccacctgcca tcacaactgg
    34261 atagagtttc gactgcgcca taattatccc cgaatatcat ttataaattg acgtaacacg
    34321 ttctctttat cgagcgtgcg ttcggcatat tcacgtgcca ccgtgttatg tttgggcagc
    34381 aggagcgcct gacgaatccc cgccaccagc gcctcgaccg attccggttc tacgcaaacc
    34441 gcaatacccg gaaaggtttc gcaaagctgc cccagttctg tgtgggcctc tgcggtaatc
    34501 accgcgttac cgcctaccgc cagaatattg gtcagtttcg acggcaatac ggcatctgcc
    34561 gcgccgcgtt tttgcaccac cagatggcaa tcgcccatct tcagcagtgc aggtaaagcg
    34621 tcatacgatt gcagcgggaa aaattgcatg ttgcgcagtc cacgctgctg cgccattttt
    34681 tccagccgcg ctttgccgcc gccctgcccg acaatggcaa aaatcagcgg ttcatcgcgc
    34741 aggagatcgg cagcttcaat aacgttttcc agcccctgct tttcaccaat attgccggag
    34801 taaagaatga tttttttgtt atccggcagg ccaagctggt tacgaagggc atcaacatcg
    34861 gcgtctgcaa catgctgaaa acgggcaatt tccgaccagt tggggaagaa gatgacgtta
    34921 tccgccgcca cgcctttttc gatggcttta ttcatcatcg aacgcgaaat cgtggaaacg
    34981 ttatcgacgt tatgcagtcc gctacgttcg aacgccgttg ccagctgtgc cactttgccg
    35041 cctttgcctt ttccggcaag gcccagcccc agcatggcgt ccacttcgta atcctgaata
    35101 tgcagcacgg tacgcgcacc agagagcttc gccagcaggc gcattcccgg cgtgcaaaac
    35161 agcgttggca ctacgccaat aatgcgatcc ggcttccagc gacgttgcgc catcagtgga
    35221 aagaaactgc tgacggcaaa actgccgaga tgcaacaagc gtttcagggt gctcggctgt
    35281 tttggcacgt acagcgggca gcgccacacc gtggcggccc cttcttctcg tttgtagcgc
    35341 caggcggaat agttctcacc cacctgccat tgcgggtagt aaggcggtgc ggtaatgacc
    35401 cgcacctcat gaccttgtgc cgccaaccat tccaccatct cgccggtgta tttgccgatg
    35461 ccggttaact ccggcgagta gttaatgccg tagaccagta ttttcataat ccgggtactc
    35521 cggcacgctt ctcagcgaga aaataggctc ggctgttagc gtgaacatcg ttactggcga
    35581 gcagcgcgtc cggcgtcagc cagcggtaat cgtcatgctg ctcatccggc agtaacagct
    35641 cttcttccgc tactctgaag cgaaaaccga gcaccacata gtgagtggtg aaatccgtgc
    35701 cggagaagtt atcgtcataa aaatgctgcc agacaccgta aaactggcct gctgttatcg
    35761 gcagacgcag ccccagttcc gccatcgtca gccgctcaaa tgcggcttcc agcgtttcgt
    35821 ctttctgcac gcgccctccc ggcacaaacc agtaaccctg cgccgggcgg ttggttcttt
    35881 tgccaagcag aaactcgccg cgactgttct cgacaataaa gtcgagagag acaagcggtg
    35941 tggagcgcac tacagtggca aagtcttcct gacgtaaaaa catcattccc cccgaaagcg
    36001 gtcttgattc tcaaggaacc actggtaagt gctggcaagc cccgcttcca gtgagatttc
    36061 gtgataccag ccaagctgat gcaggcgcgt cacatccagc agtttgcgcg gcgtaccatc
    36121 cggtttgctg gcatcaaaaa ccacccgacc tttgtaaccc accactttgg cgatggtttg
    36181 cgccagctcg cggatagtgc agtcaacgcc cgtgccgacg ttaatgtgcg acagcatcgg
    36241 ttgggtattc tccagccaga cttcatgcgc cagctccatg acatgaatgc tcgccgccgc
    36301 catatcatcg acgtgcagga attcacgcat cggtgtaccg ctgccccata ccaccacgtc
    36361 cggcgcattc tgtgccgtcg cctcgtggaa gcgacgcagc aatgctggta tcacatggga
    36421 gttactcggg tggaagttgt catgcggccc gtacaggttg gtcggcatga cagtgcggta
    36481 atcgcgtcca tactggcggt tgtaagattc gcacagtttg atcccggcga ttttggcaat
    36541 ggcgtaaggc tcgttggtcg gctccagcgt gccctgcaat aactcgcttt ctgccatcgg
    36601 ctgttttgcc agtttcgggt agatgcagga cgatccgaga aacatcagtt tgttcacgtc
    36661 gttctgatgc gcggcgtgaa tgatgttgct ctcaatcatc atgttctggt agatgaaatc
    36721 cgccggatag gtgttgttgg caacaatgcc gcccactttc gccgccgcca gatagacctg
    36781 gtcaatgcgt tcgctggcaa agaaatcatg cacggcgcgg ctgtccagca ggttcagttc
    36841 atcgcgggta cgtaatacca gctccacatc accgcgctgt tcgagctgcc gcttgatggc
    36901 agaaccgacc atcccacaat ggccagcgat aaaaatgcgt tgtttactca tgcttatgac
    36961 tccagcgcga tcgccacgtc gtagccgtga gatttcagca gagagtgttt tttcgccgct
    37021 tcgaggtcat tagccaccat ttcagacacc atctctctga gggtgatttc cggtttccag
    37081 cccagttttt cgtgcgcttt ggtcgggtcg ccgagcagcg tttcaacttc tgccggacgg
    37141 aagtaacgcg gatcaacggc gataatcaca tcacccggtt taacgcccgg cgcgtcatgc
    37201 ccggtgacgg aaaccacaat gcccttctct tcaacacccg tgccttcaaa gcgcagtttg
    37261 atgcccagct gtgctgccgc catttccaca aactgacgta cggagtactg aacgccggtc
    37321 gcgataacga aatcttccgg ctgttcctgt tgcagcatca tccactgcat ttttacgtag
    37381 tctttggcgt ggccccagtc acgcagggaa tccatattgc cgaggtacag gcacgactcc
    37441 agcccctggg cgatgttggc gattgcgcgg gtgattttgc gggtaacgaa ggtttcgccg
    37501 cggcgcgggg attcatggtt gaagagaatt ccgttacaag cgtacatgcc gtaggattcg
    37561 cggtagttaa cggtgatcca gtaggcgtac agtttggcga ccgcatacgg agatcgcggg
    37621 tagaacggcg tggtttcttt ctgcggaatt tcctgcacca gaccatacag ttcagaggtg
    37681 gaagcctgat agaaacgagt tttcttttcc agaccgagga agcggatcgc ctccagcagg
    37741 cgcagcgtac ccatcgcgtc gacgtcagcg gtatattctg gtgactcaaa agagaccgca
    37801 acgtggctca ttgcgcctag gttgtacact tcatccggct gtacttcacg caaaatgcgc
    37861 gtcaggttag aggtatcact caggtcgcca taatgcagat ggaatttcgg gttgcaggtg
    37921 tgcggatcct gataaatgtg atccacgcgc tcggtgttga atgacgatgc gcgacgctta
    37981 ataccatgca cctcgtaacc tttttccagc agaaactctg ccaggtaaga accgtcttgt
    38041 ccggttacac cggtgatgag agcgactttt gacatgtctt attcctctgt atttttgaat
    38101 ttattcagtt tcaacgcgtt cgcgtatcac cactgcggga ttcccccggc aaaccacatt
    38161 tgccggaagc gatttaaaaa cactgcttcg tgcacccacg acggtgccgt cgccgattgt
    38221 gacgcctggg gcaacaaaga catcggttgc cagccagcat ttctcgccaa tcacaatagg
    38281 cgtggcgtta atggtgaaat gttgacttgc atggtcgtgg ctaccggtgc ataaataact
    38341 tttttgcgat atcaccgaat gtgcgccaat ggttatttca ccgagggtat ataaattgac
    38401 gtcatcgcca acccacgcgt aatcacctaa ggttaatttc cacggatagg taatttttac
    38461 tgacggacga ataactacgt tttttcctat ttttgctccg aataaacgta ataaaaaagc
    38521 ccgccagcga tacaatactt gtggcgacca ggcaaatatt gttgcctgta ctgcccacca
    38581 taattgcact ttaatagcgt tgccgccccg gaaccctttc ggcaccgaga aaccgcttaa
    38641 atcttgcatt ggttttcctt atattcagac tttgttatat aaggctttag tcttacctgt
    38701 cgtacgttgg cgtaaatgcc aggataattc tgcccagaag ccaggtacat gtaatatctg
    38761 ccgttggact tttttggcgt cagcac
//
LOCUS       DADLTY010000012        37723 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.012, whole genome shotgun sequence.
ACCESSION   DADLTY010000012 DADLTY010000000
VERSION     DADLTY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.012"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..691)
                     /locus_tag="JN334_000550"
     CDS             complement(<1..691)
                     /locus_tag="JN334_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436808.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGEL"
     gene            complement(691..2253)
                     /gene="trpE"
                     /locus_tag="JN334_000551"
     CDS             complement(691..2253)
                     /gene="trpE"
                     /locus_tag="JN334_000551"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAY7436809.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            complement(2345..2389)
                     /gene="trpL"
                     /locus_tag="JN334_000552"
     CDS             complement(2345..2389)
                     /gene="trpL"
                     /locus_tag="JN334_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HAY7436810.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            2527..3408
                     /gene="yciV"
                     /locus_tag="JN334_000553"
     CDS             2527..3408
                     /gene="yciV"
                     /locus_tag="JN334_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HAY7436811.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            3405..4025
                     /locus_tag="JN334_000554"
     CDS             3405..4025
                     /locus_tag="JN334_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAY7436812.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            4126..4998
                     /gene="rluB"
                     /locus_tag="JN334_000555"
     CDS             4126..4998
                     /gene="rluB"
                     /locus_tag="JN334_000555"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HAY7436813.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(5038..5628)
                     /gene="cobO"
                     /locus_tag="JN334_000556"
     CDS             complement(5038..5628)
                     /gene="cobO"
                     /locus_tag="JN334_000556"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAY7436814.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(5625..6383)
                     /locus_tag="JN334_000557"
     CDS             complement(5625..6383)
                     /locus_tag="JN334_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HAY7436815.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6603..7652
                     /gene="sohB"
                     /locus_tag="JN334_000558"
     CDS             6603..7652
                     /gene="sohB"
                     /locus_tag="JN334_000558"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAY7436816.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(7688..7939)
                     /locus_tag="JN334_000559"
     CDS             complement(7688..7939)
                     /locus_tag="JN334_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HAY7436817.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            8319..10916
                     /gene="topA"
                     /locus_tag="JN334_000560"
     CDS             8319..10916
                     /gene="topA"
                     /locus_tag="JN334_000560"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAY7436818.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            11126..12100
                     /gene="cysB"
                     /locus_tag="JN334_000561"
     CDS             11126..12100
                     /gene="cysB"
                     /locus_tag="JN334_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HAY7436819.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            12395..12559
                     /locus_tag="JN334_000562"
     CDS             12395..12559
                     /locus_tag="JN334_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HAY7436820.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            12562..12729
                     /locus_tag="JN334_000563"
     CDS             12562..12729
                     /locus_tag="JN334_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436821.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            12843..12938
                     /locus_tag="JN334_000564"
     CDS             12843..12938
                     /locus_tag="JN334_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436822.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            13102..15777
                     /gene="acnA"
                     /locus_tag="JN334_000565"
     CDS             13102..15777
                     /gene="acnA"
                     /locus_tag="JN334_000565"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HAY7436823.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(15841..16431)
                     /gene="ribA"
                     /locus_tag="JN334_000566"
     CDS             complement(15841..16431)
                     /gene="ribA"
                     /locus_tag="JN334_000566"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAY7436824.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            16601..17365
                     /gene="pgpB"
                     /locus_tag="JN334_000567"
     CDS             16601..17365
                     /gene="pgpB"
                     /locus_tag="JN334_000567"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HAY7436825.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            17514..17822
                     /gene="lapA"
                     /locus_tag="JN334_000568"
     CDS             17514..17822
                     /gene="lapA"
                     /locus_tag="JN334_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HAY7436826.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            17829..18998
                     /gene="lapB"
                     /locus_tag="JN334_000569"
     CDS             17829..18998
                     /gene="lapB"
                     /locus_tag="JN334_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAY7436827.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            19191..19928
                     /gene="pyrF"
                     /locus_tag="JN334_000570"
     CDS             19191..19928
                     /gene="pyrF"
                     /locus_tag="JN334_000570"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAY7436828.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            19928..20254
                     /gene="yciH"
                     /locus_tag="JN334_000571"
     CDS             19928..20254
                     /gene="yciH"
                     /locus_tag="JN334_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HAY7436829.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(20380..20598)
                     /gene="osmB"
                     /locus_tag="JN334_000572"
     CDS             complement(20380..20598)
                     /gene="osmB"
                     /locus_tag="JN334_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HAY7436830.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(20867..21616)
                     /locus_tag="JN334_000573"
     CDS             complement(20867..21616)
                     /locus_tag="JN334_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7436831.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(21706..21879)
                     /locus_tag="JN334_000574"
     CDS             complement(21706..21879)
                     /locus_tag="JN334_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436832.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            complement(22027..22807)
                     /locus_tag="JN334_000575"
                     /pseudo
     CDS             complement(22027..22807)
                     /locus_tag="JN334_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548491.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(22820..23521)
                     /locus_tag="JN334_000576"
                     /pseudo
     CDS             complement(22820..23521)
                     /locus_tag="JN334_000576"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(23540..24001)
                     /locus_tag="JN334_000577"
     CDS             complement(23540..24001)
                     /locus_tag="JN334_000577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436833.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDALRFSTTKTTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIG"
     gene            24055..24158
                     /locus_tag="JN334_000578"
                     /pseudo
     CDS             24055..24158
                     /locus_tag="JN334_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24236..26170)
                     /gene="rnb"
                     /locus_tag="JN334_000579"
     CDS             complement(24236..26170)
                     /gene="rnb"
                     /locus_tag="JN334_000579"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HAY7436834.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(26238..27365)
                     /locus_tag="JN334_000580"
     CDS             complement(26238..27365)
                     /locus_tag="JN334_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HAY7436835.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            complement(27509..28297)
                     /gene="fabI"
                     /locus_tag="JN334_000581"
     CDS             complement(27509..28297)
                     /gene="fabI"
                     /locus_tag="JN334_000581"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAY7436836.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            complement(28665..29018)
                     /locus_tag="JN334_000582"
     CDS             complement(28665..29018)
                     /locus_tag="JN334_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HAY7436837.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            complement(29086..29892)
                     /gene="sapF"
                     /locus_tag="JN334_000583"
     CDS             complement(29086..29892)
                     /gene="sapF"
                     /locus_tag="JN334_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HAY7436838.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(29894..30886)
                     /gene="sapD"
                     /locus_tag="JN334_000584"
     CDS             complement(29894..30886)
                     /gene="sapD"
                     /locus_tag="JN334_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HAY7436839.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(30886..31776)
                     /gene="sapC"
                     /locus_tag="JN334_000585"
     CDS             complement(30886..31776)
                     /gene="sapC"
                     /locus_tag="JN334_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HAY7436840.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(31763..32728)
                     /locus_tag="JN334_000586"
     CDS             complement(31763..32728)
                     /locus_tag="JN334_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antimicrobial peptide ABC transporter permease
                     SapB"
                     /protein_id="HAY7436841.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(32725..34368)
                     /gene="sapA"
                     /locus_tag="JN334_000587"
     CDS             complement(32725..34368)
                     /gene="sapA"
                     /locus_tag="JN334_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HAY7436842.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(34681..34926)
                     /locus_tag="JN334_000588"
     CDS             complement(34681..34926)
                     /locus_tag="JN334_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HAY7436843.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(35060..36445)
                     /gene="puuP"
                     /locus_tag="JN334_000589"
     CDS             complement(35060..36445)
                     /gene="puuP"
                     /locus_tag="JN334_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HAY7436844.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(36435..36599)
                     /locus_tag="JN334_000590"
     CDS             complement(36435..36599)
                     /locus_tag="JN334_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436845.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(36748..>37723)
                     /locus_tag="JN334_000591"
     CDS             complement(36748..>37723)
                     /locus_tag="JN334_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAY7436846.1"
                     /translation="QRGLFPVVAVELEFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQV
                     YSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYHTDNVLEACDDALA
                     LKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENVLSDTEGEDSPLLK
                     KMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPCGDRHNH
                     RVEYHVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQEGLPFPIRQSDAL
                     GEFIENDHLRRYLGERFCHVYHACKNDELLQFERLITETEIEWMLKNA"
BASE COUNT         9476 a         9385 c         9819 g         9043 t
ORIGIN      
        1 tcagctcgcc acgcaccacc gctgaaaaca gctggtggct ttcttgttgg ctaagcgtct
       61 gcgcctgata cagtttttcc agaatcggtt gcagcgtgtt ggctggctct agtttctgct
      121 gcgcccaggc cagcgtttgt tccagcaggc gagcgccctg ggtggtgaga atggattccg
      181 gatggaactg gaatccacaa acgcgatccg catcgtgacg tactgccatc accatgccat
      241 taaaatgggc gttgatggtt aaaccggccg gaatgttact gccaaccagc gagtgataac
      301 gcgccaccgg cagcgggttt gttaatccgg caaacatcgc ctgaccgtca tgttcaatgc
      361 tcgacgcttt accgtgaaga atttcgcccg cctgaccgac atagcccccg taagcttcga
      421 caattgcctg atgtccgagg caaatgccaa taatgggcag cttgccacgc aagcgggtga
      481 ggagttccgg catacaaccg gcttcgctcg gcacaccggg gccaggagaa agcatcagta
      541 ccggattgct catcgtcgcc aggcgttcaa ttaaggtctg cgccggaata tggttgcggt
      601 aaatcaccac gttatgacca ttgctgcgca actgatctgc caggttgtac gtaaaagagt
      661 cgatattatc gagcagcaga atgtcagcca tcagaaagtc tcctgtgcat gatgcgcggt
      721 ggcaatagcg cgcagtacag cgcgggcttt gttacgggtt tcgtcggctt ccgactgcgg
      781 aacagaatca aggactacac cagcacccgc ttgcacggtg gcgataccgt tttccaccag
      841 cgccgagcgg atcacaatgc aggtgtcgag atcgccatgc gcggtgaaat aacctaccgc
      901 gccgccgtag ctgccgcggc gacgaccttc cgcctcggca attaactgca tagcgcgtac
      961 tttcggcgca ccgcttaacg tccccatatt catacaggcg cgataagcgt gcagggcatc
     1021 aagatcgtga cgcagttcgc cgactacgcg agagacgagg tgcatcacat aggaataacg
     1081 gtcaactttg gtgagatcgg cgatgtagcg gctgccaggg gtgcaaatgc gtgccagatc
     1141 attacgggcg agatcaacca gcatcagatg ttcagacagc tctttatggt cagtacgcat
     1201 ttccagttca atacggctgt cgagatctct gtccagtgaa ccatcggcgc ggcgaccgcg
     1261 tgggcgtgtt ccggcaatcg ggtagatctc aatctggcgg ctggtggcat catacttgag
     1321 cgagctttcc ggcgacgcgc caaatagggt gaaatcatta tcctgcataa aaaacatgta
     1381 cgggctggga ttactctttt tcagcacgta ataggccgcc agcggtgacg ggcagggcag
     1441 agagaaacgg cgagatggca ccacctggaa aatttctcca gcgcgaatcg ctttttgcaa
     1501 caaacgcact acgccaccga actcttcatc gctctgatta cattcacaac gcatatgcgg
     1561 cacggaaacc actggcagcg gcggcgcggc ttcggtcagt tgctgacgta gttcgttcag
     1621 gcgagcagtg agacgttgtt tttcttcttc attcggagca aacaggctgg cctgaatacg
     1681 ggtgcttttt ttctgatggt caatcaccat cagcgtttca gcgagataaa aacagaaatc
     1741 agggcagtta ttttccgctg acagttgcgg taaatcttca aatcccgcca caaggtcata
     1801 agagaacagg ccgccgaaga acattgcttc tcgttcttcc ttcggtacat tcaacagatt
     1861 ctgcaataaa cggaaagcgt caaaaaccga aagggagcat aagcgggcgt cttcatccag
     1921 cagtggactg acagggggga agcgcagcac acggcagttt ggtgattgtt cactttccac
     1981 acccgcaggc agggcgttat ccagtagtgc cagcagggct tcgccattgc cggaaagtgc
     2041 ctgaattgtg acggtgtcac ctaatgctgt aatgcgcagc gcactgtcta tcagcagcag
     2101 gctttttaaa tcatctttgc tgtcgatatc tgcggattcc agcagcagcg ttgccggacg
     2161 attcccacac aactggtgaa aaagcgcagt cgggttgtcg cgataagcgc cttcgcaggt
     2221 tagcagttcg agagtcggtt tttgtgtttg cattgttatt ctctaatttt gttcaaaaaa
     2281 aagcccgctc attaggcggg ctgggtatct gattgcttta cgcatggtga atacactgcc
     2341 cgtttcagga agtgcgccac caacctttca gtacgaaaat tgctttcatt gtcgataccc
     2401 tttttacgtg aacttgcgta ctagttaact ggttcgatga ttaattgtca acagctcatt
     2461 tcagaatatt tgccagaccc gttatgatgt cggcgcaaaa aacattatcc agaacgggag
     2521 tgcgccttga gcgacacgaa ttatgcagtg atttacgacc tgcacagcca taccacagct
     2581 tccgatggct gcctgacgcc agaagcattg gtgcaccgtg cagtcgagat gcgcgtcggc
     2641 accctggcga tcaccgacca tgacaccaca gctgcaatcg cgccagcaag agaggaaatt
     2701 tcacgttcgg gactggcgct gaatcttatt cccggcgtgg aaatttccac ggtctgggaa
     2761 aatcatgaaa ttcatattgt cgggctgaat attgatatta ctcatccgct aatgtgtgag
     2821 ttcctcgcgc agcagacaga acggcgcaat cagcgggcgc agcaaattgc cgaaagactt
     2881 gaaaaagcgc aaatacctgg ggcgctggaa ggcgcacaac gactggcgca gggcggcgca
     2941 gtgacgcgcg gtcattttgc gcgtttcctt gtggagtgcg gcaaagccag ttcaatggca
     3001 gatgtcttta aaaagtatct ggcgcgcggg aaaaccggat acgttccgcc acagtggtgt
     3061 acaatagaac aagctattga tgtcattcat cattctggcg gtaaggcggt gctagctcat
     3121 cctgggcggt acaatcttag tgctaaatgg ctgaaaagat tggttgcgca ttttgccgaa
     3181 caccacggcg acgcgatgga agtcgcgcag tgccagcaat cgcccaatga acgcacccag
     3241 ctggccacac ttgcgcgtca gcatcattta tgggcatcac aaggatctga ttttcatcag
     3301 ccatgcccgt ggatcgaact gggtcgtaaa ctctggctgc ccgcaggcgt tgaaggcgtc
     3361 tggcagctat gggaacagcc gcagaacacc acagagaggg aattatgagc cagttttttt
     3421 atattcatcc tgataacccg cagcaacgtc tgatcaacca ggcggtggag atcgtgcgta
     3481 aaggcggggt gattgtctat ccaactgatt ccggctatgc gctcggctgt aaaattgaag
     3541 ataaaaacgc catggagcgt atttgtcgta ttcgccagct gccggacggt cacaacttta
     3601 ccctgatgtg tcgcgatctt tctgaactct cgacctattc attcgtggat aacgttgcgt
     3661 ttcgtttgat gaaaaacaac acgccgggca actatacctt catcctgaag gggacgaagg
     3721 aagtgccacg ccgtctgttg caggagaaac gcaaaaccat cggtatgcgt gtgccttcta
     3781 acccgattgc gcaggcgttg cttgaagcac tgggcgaacc gatgctttcc acttcgctaa
     3841 tgctgccagg cagcgaattt accgaatccg atcccgaaga aattaaagac cgtctggaaa
     3901 aacaggtaga tttgattatt cacggcggct atctcgggca gaaaccaaca acggttattg
     3961 atcttaccga tgatacgccg gtcgtggtgc gtgaaggcgt aggtgatgtg aagcctttct
     4021 tataagtgcg taactctgta tactacgcgg ccttaaaatg gcgctatacg gcgccattga
     4081 ttctcctgtt cgacgcctgt gaaggcgaca ccaaaaggaa gctctatgag cgaaaaatta
     4141 cagaaagtgc tggcccgcgc tggccacggc tcccgccgtg aaatcgaatc tattattgaa
     4201 gccggtcgtg tgagtgttga tggcaaaatt gctaaactcg gcgatcgcgt tgaagttacc
     4261 cctgggctga aaattcgtat cgatggtcat ctgatttcgg tacgtgagtc cgctgaacaa
     4321 atttgtcgcg ttctggccta ttacaaaccg gaaggtgagt tgtgtacccg taacgatccg
     4381 gaagggcgtc cgacggtgtt tgaccgtctg ccaaagctgc gtggcgcacg ctggattgcc
     4441 gtaggtcgtc tggacgttaa cacctgtggt ctgctgctgt tcaccaccga tggtgaactg
     4501 gcaaaccgtt taatgcatcc aagccgtgaa gttgaacgtg aatacgccgt gcgtgtattt
     4561 ggtcaggttg acgacgcgaa actgcgtgat ttgagtcgtg gcgtgcaact ggaagatggt
     4621 ccggcagctt ttaaaaccat caagttcagc ggcggcgaag ggatcaacca gtggtacaac
     4681 gtgactctga ccgaaggccg taaccgtgaa gttcgtcgtc tgtgggaagc ggttggtgtg
     4741 caggtgagcc gcctgatccg tgttcgttac ggtgatatcc cactgccaaa aggtctgcca
     4801 cgcggtggct ggacggagct ggacctcgcc cagactaact atctgcgcga actggtggag
     4861 cttccgccag aaaccagctc taaagtcgct gtagaaaaag accgtcgtcg catgaaggct
     4921 aatcagattc gtcgtgcggt gaaacgtcac agtcaggtga gcggcagtcg tcgttctggc
     4981 gggcgtaata acggttaatc aaagtatgcc ggacgtcata tccggcattt ttacagatta
     5041 ataatcaatc cctatttgcg ccttaacacc ggcatcaaac gcatgtctga tggggcgcag
     5101 ctcacttacc gtatccgcca gttcaagaat atcccgatga cagcctcgac ccgtgataat
     5161 caccgtctgt tgatgtggac gttcatttaa cgcctgcacc acttcttcca gtggcaaata
     5221 gtcatacgcc accatatacg tcagttcatc aagcaaaacc atatccagtg aggaatcagc
     5281 aagcatccgc tttgcatgtt gccagacttc gcggcaggcg gcggtatcag actcgcggtt
     5341 ttgtgtatcc caggtaaagc ccgttgccat cacctgaaac tcaacgccat gtggctccag
     5401 cagattgcgt tcgccattag gccaggtgcc tttaataaac tgcacgacgc ccactttttt
     5461 tccgtgacca actgcgcgtg ttgccgtacc aaatgccgcg gtggttttgc cttttccatt
     5521 gccggtaaag acgatgataa taccgcgttc atcctgggcc tgggccacac gagcatctac
     5581 tttttctttc actcgctgct gacgctgttg gtagcgttca tcactcattg ggaaattcct
     5641 ggtttacggc ccggttgggc gtcaaaggtc atgccggttt tacggcggct gtcatcgccc
     5701 atcagccaga ggtagagcgg catgatatca gcgggtgttt taagtttctg tggatcttcg
     5761 gtcgggaagg cgcttgcgcg cattgcggtg cgcgtaccgc ctgggttaat gcagttgacg
     5821 cgcaggcgct gctgatattc atcggccagt acctgcatca tcccttcggt ggcaaatttc
     5881 gacgctgcat atgcacccca gttggctcgt ccctgacgtc caacgcttga tgaagtaaag
     5941 accagtgaac cggcgtccga tttgagtaat aaaggaagca gtgcctgggt gagcataaag
     6001 gtggcattaa cgttgatctg catgacgtcc tgccagacct gcggattttg ttcgctcatt
     6061 gggcaaacat cgccgagcaa tccggcatta tgcaaaacac catccagacg cggataatta
     6121 acgacaatgc gctgtgccag ttgttggcaa ttttcggacg tgcaggtcag caaatcgaga
     6181 ataaaccact gtggctgacg cccagtttct tcgtttatgt ggctggctac ctgacgtaat
     6241 ttttcttcat tacggcccaa cagaatcact gtcgcaccat agcgtgcata cgtcatcgcg
     6301 gcttcacgac caatcccatc gctggctccc gtcaccagga taatgcgatc attgagtaaa
     6361 tcttgttttg gctggtaatg catggctact cctcaacgac gttgtctgtc gcgctgccag
     6421 tgtactttat gactttgggg ctttatgcct gaaacagaag ggcatttcaa tcagcctggg
     6481 gcgataacac cgcaaaatgg atactttgtc atactttcgc tgcaataaca tctctgcgag
     6541 acggcttaac atgcctgttg taaactgtga gccaaagcgt tgtttaacca aggtggggac
     6601 tcgtggaatt gttgtctgaa tatggtttgt ttttggctaa aatcgttacc gttgtgctag
     6661 cgattgcggc gattgccgcc attattgtca atgttgctca acgtaataaa cgccagcgtg
     6721 gcgagttacg ggtcaacaat ctcagcgaac agtataagga gatgaaagaa gaactggccg
     6781 cggcgctgat ggactcacat cagcaaaaac agtggcacaa agcgcagaag aaaaagcaca
     6841 agcaagaagc gaaagcagca aaagcgaaag ccaaactggg cgaggtggca actgacagta
     6901 aacctcgcgt ctgggtgctg gattttaaag gcagcatgga cgcccatgaa gtgaactcgc
     6961 tacgtgaaga gataacggcg gtactcgcag cattcaaacc gcaggatcag gttgtgctcc
     7021 gtctggaaag ccctggtggc atggtgcatg gttacgggtt ggcggcttcg cagctgcagc
     7081 gtctgcgtga taaaaacatt cctttaactg ttacggtaga caaagtcgct gccagcggcg
     7141 gttacatgat ggcctgtgtg gcggacaaaa ttgtttccgc accgtttgct attgtgggtt
     7201 ccattggggt ggtggcgcaa atgcccaact ttaaccgctt cctgaaaagc aaagatattg
     7261 atatcgaact gcacaccgcc gggcagtata agcgcacgct gaccttgctg ggtgaaaata
     7321 ccgaagaagg gcgggagaaa ttccgcgaag agctgaacga aacgcatcag ttatttaaag
     7381 attttgtgaa gcgtatgcgt ccgtctctgg atattgaaca ggtggcaacg ggtgaacact
     7441 ggtacggaca acaggcggta gagaaaggcc tggttgatga aatcaacacc agtgatgaag
     7501 ttattcttag cctgatggaa ggccgtgaag tggttaatgt acgctatatg cagcgtaaac
     7561 gactcattga ccgattcacc ggcagcgcgg cagagagcgc cgatcgattg ttgctacgct
     7621 ggtggcagcg gggtcaaaag ccattgatgt aaaagacaaa cgcgaggcta agacctcgcg
     7681 ttttgcttta atcaaccaga tgatattttt ctgaaagcac atgggccagg tgtttgaaca
     7741 tattaaacac cgcggtgctt ttggctgttg gcaatccttg ttcatctaaa aagtagtcgc
     7801 cggtaaatac cagcacgcca ttacgctgcg tgacgccggt ggcttcaatc cctgcgagcg
     7861 tatcctcatg ctcacgaatg attttgttgg cctctttcaa cagcgtttcg cggtcgatgg
     7921 gttgtgtctc tttgttcatt atttactcct taaacaagga cattagtcta cgccaggcat
     7981 ggcttgcaga caaatatacc acgctggtgg caagagcgcc ttactggcaa ctttggattt
     8041 tgcatgctaa taaagttgcg tatcggattt tatcaggtac agtgtgacgc tttcgtcaat
     8101 ctggcaatag atttgcttga cattcgacca aaattccgtc gtgctatagc gcctgtaggc
     8161 caagacctgt taactcagtc acctgaattt tcgtgaacag agtcacgaca aggggttgat
     8221 atccgcagag agcgagtcca tatcggtaac tcgttgccag tggaaggttt atcaacgtgc
     8281 gacgcattcc tggaagaatc aaattaggta aggtgaatat gggtaaagct cttgtcatcg
     8341 ttgagtcccc ggcaaaagcc aaaacgatca acaagtatct gggttgtgac tacgtggtga
     8401 aatccagcgt cggtcacatc cgcgatttgc cgaccagtgg ctcagctgcc aaaaagagtg
     8461 ccgactctac ctccaccaag acggctaaaa agcctaaaaa ggatgaacgt ggcgctctcg
     8521 tcaaccgtat gggggttgac ccgtggcaca attgggaggc gcactatgaa gtgttgcctg
     8581 gtaaagagaa ggtcgtctct gaactgaaac aactggctga aaaagccgac cacatctatc
     8641 tcgcaaccga ccttgaccgc gaaggggaag ccattgcatg gcacctgcgg gaagtgattg
     8701 gtggtgatga tgcgcgctat agccgagtgg tgtttaacga aattactaaa aacgcgatcc
     8761 gccaggcatt taacaaaccg ggtgagctga atattgatcg tgttaatgcc cagcaggcgc
     8821 gtcgctttat ggaccgcgtg gtggggtata tggtttcgcc gctgctgtgg aaaaagatcg
     8881 ctcgtggtct gtctgccggt cgtgtgcagt cggtggcggt ccgcctggtg gtagagcgtg
     8941 agcgtgaaat taaagcgttc gtaccggaag agttctggga agtcgatgcc agcacgacta
     9001 cgccatctgg tgaagcgttg gcgttgcagg tgactcatca gaatgacaaa ccgttccgtc
     9061 cggtcaacaa agaacaaact caggctgcgg taagtctgct ggaaaaagcg cgctacagcg
     9121 tgctggaacg tgaagacaaa ccgacaacca gtaaacctgg cgctcctttt attacctcta
     9181 cgctgcaaca agctgccagc acccgtcttg gttttggcgt gaaaaaaacc atgatgatgg
     9241 cgcagcgttt gtatgaagca ggctatatca cttacatgcg taccgactcc actaacctga
     9301 gtcaggacgc ggtaaatatg gttcgcggtt atatcagcga taattttggt aagaaatatc
     9361 tgccggaaag tccgaatcag tacgccagca aagaaaactc acaggaagcg cacgaagcga
     9421 ttcgtccttc tgacgtcaat gtgatggcgg aatcgctgaa ggatatggaa gcagatgcgc
     9481 agaaactgta ccagttaatc tggcgtcagt tcgttgcctg ccagatgacc ccagcgaaat
     9541 atgactccac gacgctgacc gttggtgcgg gcgatttccg cctgaaagca cgcggtcgta
     9601 ttttgcgctt tgatggctgg acaaaagtga tgcctgcact gcgtaaaggc gatgaagatc
     9661 gtatcttacc ggcagtcgat aaaggcgatg ctctgacgct cgttgaacta acaccagccc
     9721 agcactttac caagccgcca gcccgtttca gtgaagcatc gctggttaaa gaactggaaa
     9781 aacgtggtat cggtcgtccg tctacctatg cgtcgatcat ttcgaccatt caggatcgtg
     9841 gctatgtgcg agtagaaaat cgtcgtttct atgcggaaaa aatgggcgaa atcgtcaccg
     9901 atcgccttga agaaaatttc cgcgagttaa tgaactacga ctttaccgcg cagatggaaa
     9961 acagcctcga ccaggtggca aatcacgaag cagagtggaa agctgtactg gatcacttct
    10021 tctcggattt cacccagcag ttagataaag ctgaaaaaga tccggaagag ggtggtatgc
    10081 gcccgaacca gatggttctg accagcattg actgcccgac ttgtggtcgc aaaatgggga
    10141 ttcgcacagc gagtaccggg gtattccttg gctgttctgg ctatgcgctg ccgccgaaag
    10201 agcgttgcaa aaccaccatt aacctggtgc cggaaaacga agtgctgaac gtgctggaag
    10261 gcgaagatgc tgaaaccaac gcgctgcgcg caaaacgtcg ttgcccgaaa tgcggcacgg
    10321 cgatggacag ctatctcatc gatccgaaac gtaagttgca tgtctgtggt aataacccaa
    10381 cctgcgacgg ttacgagatc gaagagggcg aattccgcat taaaggttat gacggcccga
    10441 tcgttgagtg tgaaaaatgt ggctctgaaa tgcacctgaa aatggggcgt ttcggtaaat
    10501 acatggcctg caccaacgaa gagtgtaaaa acacacgtaa gattttacgt aacggtgaag
    10561 tggcaccacc gaaagaagat ccggtgccat tacctgagct gccgtgcgaa aaatcagatg
    10621 cttatttcgt gctgcgtgac ggtgctgccg gtgtgttcct ggctgccaac actttcccga
    10681 aatcgcgtga aacgcgtgcg ccactggtgg aagagcttta tcgcttccgc gaccgtctgc
    10741 cggaaaaact gcgttatctg gccgatgcgc cacagcagga tccggaaggt aataagacca
    10801 tggttcgctt tagccgtaaa accaaacagc aatatgtctc ttcggaaaaa gacggaaagg
    10861 cgactggctg gtcagcattt tatgttgatg gcaaatgggt tgaaggaaaa aaataacctt
    10921 taattctgtc aggtttttat aaacaaaggg tcgcgaaagc ggcccttttt tattgcatat
    10981 tattttttct tcacacctat acactaaggc tataaatgat atagtggtta tagttagcac
    11041 cttttttatt attaaatcgt attagtcacc cgccaggtgt gacgaaaaaa cgatgttctg
    11101 atggcgtcta agtggatggt ttaacatgaa attacaacaa cttcgctata ttgttgaggt
    11161 ggtcaatcat aacctgaatg tctcatcaac agcggaagga ctttacacat cacaacccgg
    11221 gatcagtaaa caagtcagaa tgctggaaga cgagctaggc attcaaattt tttcccgaag
    11281 cggcaagcac ctgacgcagg taacgccagc aggacaagaa ataattcgta tcgctcgcga
    11341 agtcctgtcg aaagtcgatg ccataaaatc ggttgccggt gagcacacct ggccggataa
    11401 aggctcgctg tatatcgcca ccacgcatac ccaggcacgc tacgcgttac caaacgttat
    11461 caaaggcttt attgagcgtt atccccgcgt ttctttgcat atgcaccagg gcccgccgac
    11521 acaaattgct gatgccgtct ctaaaggcaa tgccgatttc gctatcgcca cagaagcgct
    11581 gcatctgtat gaagatttag tgatgttacc gtgctaccac tggaatcggg ctattgtagt
    11641 cactccggat cacccgctgg caggcaaaaa agccattacc attgaagaac tggcgcaata
    11701 tccgttggtg acatatacct tcggctttac cggacgttca gaactggata ctgcctttaa
    11761 ccgcgcaggg ttaacgccgc gtatcgtttt cacggcaacg gatgctgacg tcattaaaac
    11821 ttacgtccgg ttagggctgg gggtaggggt cattgccagc atggcggtgg atccggtcgc
    11881 cgatcccgac cttgtgcgcg ttgatgctca cgatatcttc agccacagta caaccaaaat
    11941 tggttttcgc cgtagtactt ttttgcgcag ttatatgtat gatttcattc agcgttttgc
    12001 accgcattta acgcgtgatg tcgttgatgc ggctgtcgca ttgcgctcca atgaagaaat
    12061 tgaggtcatg tttaaagata taaaactgcc ggaaaaataa tttcttgcgt tattttcggc
    12121 accttttatg tagcgaaggt gccggaatat attctctttt gttacttatt tctataccgt
    12181 gcacaatatt tttatccctc atatttatag ggtaaattac ctgtaaagta gtgtctaatt
    12241 acccgcctcg caaattccag ttttcaaata tctatttcag gtcaaaagat tgaattattc
    12301 actggagacg attcgtaaat tcgctggatt tttgactaaa gtttctttag gatttgtctc
    12361 atcgtatgat taattacacc aatcattcaa cgtcttgctg ataagtgatg gcgattatat
    12421 gaggttagca atgccttctg gaaatcagga accccgccgt gatcctgaat taaaacgtaa
    12481 agcctggctg gcggtttttc ttggttctgc acttttctgg gtggttgtcg cactgctgat
    12541 ttggaaagtg tggggataac tatggtcggt caggagcaac tggagtcgtc accattatgc
    12601 cagcatagtg acaatgaacc agaaacgaaa cgggaatgtt ccgtcgttat tccagacgac
    12661 tggcaactaa catcgcagca gcaagccttt atagaactgt ttgctgaaga tgatcagccg
    12721 aaacaataat tatcatcatt cttattaccc atttttaatg aattaaaggg cttttaatac
    12781 accgcagcaa taacagcttg agttatctca acacaaaata ataaccgtta agggtgtagc
    12841 ctatgatcaa cacaaatatg aaatattggt cctggatggg cgcgttttct ctgtcgatgc
    12901 tcttctgggc cgaactcctc tggatcatta ctcactgatc cttgaccccg ctgcggcggg
    12961 gttgtcattt gctttgccac aaggtttctc ctcttttatc aatttgggtt gttatcaaat
    13021 cgttacgcga tgtttgtgtt atctttaata ttcaccctga agagaatcag ggcttcgcaa
    13081 ccctgtcatt aaggaggagc tatgtcgtca accctacgag aagccagtaa ggacacgttg
    13141 caggccaaag ataaaactta ccactactac agcctgccgc ttgctgctaa atcactgggc
    13201 gatatcaccc gtctacccaa gtcactcaaa gttttgctcg aaaacctgct gcgctggcag
    13261 gatggtaact cagttaccga agaggatatc cacgcactgg cgggatggct gaaaaatgcc
    13321 catgctgacc gtgaaattgc ctaccgcccg gcaagggtgc tgatgcagga ctttaccggc
    13381 gtacctgccg ttgttgatct ggcggcaatg cgcgaagcgg ttaaacgcct cggtggcgat
    13441 actgcaaagg ttaacccgct ctcaccggtc gacctggtca ttgaccactc ggtgaccgtc
    13501 gatcgttttg gtgatgatga ggcatttgaa gaaaacgtac gcctggaaat ggagcgcaac
    13561 cacgaacgtt atgtgttcct gaaatgggga aagcaagcgt tcagtcggtt tagcgtcgtg
    13621 ccgccaggca caggcatttg ccatcaggtt aacctcgaat atctcggcaa agcagtgtgg
    13681 agcgaattgc aggacggtga atggattgct tatccggata cactcgttgg tactgactcg
    13741 cacaccacta tgatcaacgg ccttggcgtg ctggggtggg gcgtgggtgg gatcgaagca
    13801 gaagccgcca tgttaggcca gccggtttcc atgcttatcc cggacgtagt gggctttaaa
    13861 cttaccggaa aattacgtga aggtattacc gccacagacc tggttctcac tgttacccaa
    13921 atgctgcgca aacatggcgt ggtggggaaa ttcgtcgaat tttatggtga tggtctggat
    13981 tcactaccgt tggcggatcg cgccaccatt gccaatatgt cgccagaata tggtgccacc
    14041 tgtggcttct tcccaatcga tgcagtaacc ctcgattaca tgcgtttaag cgggcgcagc
    14101 gaagatcagg tcgagttggt cgaaaaatat gccaaagcgc agggcatgtg gcgtaacccg
    14161 ggcgatgaac cgatttttac cagtatgtta gaactggata tgaatgacgt tgaagcgagc
    14221 ctggcagggc caaaacgccc acaggatcgc gttgcactgc ccgatgtacc aaaagcattt
    14281 gccgccagta acgaactgaa agtgaatgcc acgcataaag atcgcctgcc agtcgattat
    14341 gttatgaacg gacatcagta tcagttacct gatggcgctg tggtcattgc tgcgataacc
    14401 tcgtgcacca acacctctaa cccaagtgtg ctgatggccg caggcttgct ggcgaaaaaa
    14461 gccgtaactc ttggcctcaa gcggcaaccg tgggtcaaag cgtcgttggc accgggttcg
    14521 aaagtcgttt ctgattatct ggcaaaagcg aaactgacac cgtatctcga cgaactgggg
    14581 tttaaccttg tgggatacgg ttgtaccacc tgtattggta actctgggcc gctgcccgat
    14641 cctatcgaaa cggcaatcaa aaaaggcgat ttaaccgtcg gtgcggtgct gtccggcaac
    14701 cgtaactttg aaggccgtat ccatccgctg gttaaaacta actggctggc ctcgccaccg
    14761 ctggtggttg cctatgcgct ggcgggaaat atgaatatca acctggcttc tgagcctatc
    14821 ggccatgatc gcaaaggcga gccggtttat ctgaaagata tctggccatc ggcacaagaa
    14881 attgcccgtg cggtagaaca agtctccaca gaaatgttcc gcaaagagta cgcagaagtt
    14941 tttgaaggca cagcagagtg gaaggaaatt aacgtcacac gatccgatac ctacggttgg
    15001 caggaggact caacctatat tcgcttatcg cctttctttg atgaaatgca ggcaacacca
    15061 gcaccagtgg aagatattca cggtgcgcgg atcctcgcaa tgctggggga ttcagtcacc
    15121 actgaccata tctctccggc gggcagtatt aagcccgaca gcccagcggg tcgatatcta
    15181 caaggtcggg gtgttgagcg aaaagacttt aactcctacg gttcgcggcg tggtaaccat
    15241 gaagtgatga tgcgcggcac cttcgccaat attcgcatcc gtaatgaaat ggtgcctggc
    15301 gttgaagggg ggatgacgcg gcatttacct gacagcgacg tagtctctat ttatgatgct
    15361 gcgatgcgct ataagcagga gcaaacgccg ctggcggtga ttgccgggaa agagtatgga
    15421 tcaggctcaa gccgtgactg ggcggcaaaa ggacctcgtc tgcttggaat tcgtgtggtg
    15481 attgccgaat cgtttgaacg aattcaccgt tcgaatttaa ttggcatggg catcctgccg
    15541 ctggaatttc cgcaaggcgt gacgcgtaaa acgttagggc taaccgggga agagaagatt
    15601 gatattggcg atctgcaaaa tctacaacct ggcgcgacgg ttccggtgac gcttacgcgc
    15661 gcggatggta gccaggaagt cgtaccctgc cgttgtcgta tcgacaccgc gacggagttg
    15721 acctactacc agaacgacgg cattttgcat tatgtcattc gtaatatgtt gaagtaacaa
    15781 gtatttgctt gccggttatt ttgcttccgg caagcaaatg aattacacga tgcaagaggg
    15841 ttatttgttc agcaaatggc ccattttctc ggctttggta tcgagataat gttcgttatt
    15901 ggggttacga cctacaatca atggtacgcg ttcaacaata ttaatccctg cttcggtcag
    15961 aatttcgact tttttcgggt tattggttaa caagcggact tcattgacgc caaggagttt
    16021 gaacatatca gcgcaaagag tgaagtcgcg ctcatcagcg gcgaagccta actggtggtt
    16081 agcctctacg gtatcgtaac cttgatcctg cagtgcgtaa gcgcggattt tattcagcag
    16141 accaatgtta cgaccttcct gacggtgata cagcaaaata ccacggcctt cctcggcaat
    16201 ttgcgtcaat gccgcttcga gctggaagcc acaatcgcag cgcaagctga acagggcgtc
    16261 accggtcaga cattcggaat ggacgcgcgc aagtaccggg gtatgcccgg aaatatcgcc
    16321 atagactagc gcaacatgat cgtgtccggt tgccagttct tcaaatccca ccatcaggaa
    16381 atcgccccat ggggttggca gtttggcttc tgccacacgt ttaagctgca tgaaattctc
    16441 cagataatgc tggttctgta ttggcttatt ttgccataac gagaaggggt tcacctaatc
    16501 acgacgcgtc gatcgttcac ggaatggcac aaatctgtca atttttcttg gaactggcat
    16561 tttcagttat gattgtggga cttatcaaaa aggagaggcc atgcgttcga ttgcccgacg
    16621 taccgcagtg ggagctgcac tattgcttgt catgccagta gccgtatgga tttctggctg
    16681 gcgttggcaa cctggagaac aaagttggct actaaaagcg gctttttggg ttactgaaac
    16741 tgtcacccag ccctggggcg tcattacaca tttgattttg ttcggctggt ttctctggtg
    16801 tctgcgtttt cgcattaagg ctgcctttgt attatttgcc attctggcgg cggcaatcct
    16861 tgttggacaa ggcgttaaat cctggatcaa agacaaagta caggaaccac gaccttttgt
    16921 tatctggttg gaaaaaacac atcatattcc ggttgatgag ttctacactt taaagcgagc
    16981 agaacgcgga aatctagtaa aagaacagtt ggctgaagag aaaaatattc cacaatattt
    17041 gcgttcacac tggcagaaag agacggggtt tgcctttcct tccggtcaca cgatgtttgc
    17101 tgccagttgg gcactgctgg ccgttggttt gctgtggccg cgtcggcgaa cgttaaccat
    17161 tgctatcttg ttggtctggg caacgggagt catgggaagc cgcctgctgc tcgggatgca
    17221 ttggccacgc gatctggtgg tagctacgtt gatttcgtgg gcgctggtgg cggtggcaac
    17281 gtggcttgcg caacgaattt gtgggccatt aacaccacct gcggaagaaa atcgcgaaat
    17341 tgcacaacga gaacaagaaa gttaacgctg gttgattttc cgaatttagg ccttaaatca
    17401 tcaacaatgc gcgtggatgc catttcgcag acggcgcgaa aatggtactt taaagggcta
    17461 ttgcggtaag ttgaccataa tttattcgct ctaaccacat aacgggaagt aatgtgaaat
    17521 atttactcat tttcttactg gtgttagcga tcttcgtgat ttcggtcacg ttgggtgcgc
    17581 agaacgatca acaggtgacg tttaattatc tgttagcgca aggggagtac cgtatttcca
    17641 cattgctggc ggtattgttt gctgcggggt ttgctatcgg ttggttgatt tgtggcctgt
    17701 tctggctgcg agttcgtgtt tccctggcgc gcgctgaacg taaaataaag cgactggaaa
    17761 accagctttc acccgcgact gacgtggctg tagtgccgca ctcgtcagcg gcgaaggaat
    17821 aactttctat gctggagttg ttgtttctgc ttttgcctgt agccgctgcc tatggctggt
    17881 atatgggccg cagaagtgcg caacaaaaca agcaagatga agccaaccgc ttgtcgcgtg
    17941 attacgtagc gggggttaac ttcctgctta gtaatcaaca ggataaagcg gtagacctgt
    18001 ttctcgatat gcttaaagag gatacaggca ccgttgaagc ccaccttacg ctcggaaacc
    18061 tgttccgttc gcgtggcgaa gttgatcgcg ctattcgcat ccatcagacc ctaatggaaa
    18121 gcgcctcgct gacctatgaa cagcgtctgt tggcgattca acaactgggg cgtgattaca
    18181 tggccgccgg gttatacgac cgcgcggaag acatgttcaa tcagctgacc gatgaaactg
    18241 acttccgcat tggcgcgctg caacagttgc tacaaatcta ccaggctacc agtgagtggc
    18301 agaaagcaat tgatgttgcc gaacgcctgg tgaagctggg taaagataaa cagcgcgtcg
    18361 aaattgccca tttctactgt gagttagccc tgcagcatat ggccagcgac gatctcgatc
    18421 gtgccatgac cttgctaaaa aaaggggcgg cggcagataa aaacagcgcc cgcgtatcca
    18481 tcatgatggg acgcgtgttt atggcgaaag gagaatacgc caaagccgtc gaaagtttgc
    18541 aacgcgtcat atcccaggac agagaactgg tcagcgaaac gctggaaatg ttgcaaacct
    18601 gctatcagca gttgggtaaa actgccgaat gggcagaatt cctgcaacgc gcggtggaag
    18661 agaacaccgg tgccgatgct gaattgatgc ttgctgatat catcgaagcg cgcgacggta
    18721 gtgaggctgc acaggtctat attacacgcc agcttcagcg tcatccgacc atgcgtgtat
    18781 tccataagct aatggattac cacttaaatg aagcggaaga agggcgcgcc aaagagagtc
    18841 tgatggtgct gcgtgacatg gttggcgaga aggtgcgtag taagcctcgt tatagctgcc
    18901 agaaatgtgg ttttaccgca tacaccctct actggcattg tccgtcttgt cgtgcctggt
    18961 caactattaa accgattcgc ggtcttgatg gcctgtaatt tttaaaaaaa tccgacttta
    19021 gttacaacat actagttatt gatgtcccat tgtgctccgg caacgacgga gcagaaaaag
    19081 cctgctaggg gaaaaatcgc aactgttaat tttttatttt cacgggtaga atgctcgccg
    19141 tttacctgtt tcgcgccact cccggtgccc atcatcaaga aggtctggtc atgacgttaa
    19201 ctgcttcatc ttcttcccgc gccgttacga attctcctgt ggttgttgcc cttgattatc
    19261 ataattgtga tgacgcgctg tcctttgtcg acaagatcga cccacgcgat tgtcgtctga
    19321 aggtcggtaa agagatgttt acattgtttg ggccacagtt tgtgcgcgaa cttcaacagc
    19381 gtggttttga tatctttctt gacctgaaat tccacgatat ccccaacact gcagcgcacg
    19441 ctgtcgctgc tgcagctgac ttaggcgtgt ggatggtgaa tgttcatgcc tccggtgggg
    19501 cgcgtatgat gaccgcagcg cgtgaggcac tggttccgtt tggcaaagat gcaccgcttt
    19561 tgattgctgt gacagtgttg accagcatgg aagccagcga cctggccgat cttggcgtga
    19621 cactgtcacc tgcagattat gcagaacgtc tggcagcact gacgcaaaaa tgtgaccttg
    19681 atggtgtggt gtgttctgct caggaagctg tgcgctttaa acaggtattc ggtcaggagt
    19741 tcaaactggt tacaccgggc attcgtccgc aggggagtga agctggtgac cagcgccgca
    19801 ttatgacgcc agaacaggcg ttggcggctg gtgttgatta tatggtgatt ggtcgcccgg
    19861 taacgcaatc ggtagatcca gcgcagacac tgaaagcgat caacgcctct ttacagcgga
    19921 gtgcatgatg agtgattcca acagccgtct ggtctactca acggaaaccg ggcgtattga
    19981 tgagcccaaa gcggccccag tacgccctaa aggcgatgga gcggtgcgta ttcagcgtca
    20041 gaccagtgga cgtaaaggta agggcgtttg cctgattacc ggtatcgatc tcgatgatgc
    20101 cgaactgaca aaacttgcag cggaactgaa gaaaaaatgc ggctgcggcg gagcagttaa
    20161 agatggagtt attgaaatcc agggcgataa gcgtgattta ttaaaatcac tacttgaagc
    20221 gaaagggatg aaggtaaaac tcgcaggcgg ttaacataaa aagccacgga tatatccgtg
    20281 gctttcgaat attttactgt gtgtattatt cagttatttc gtactgaaca gaatcaagcg
    20341 tattataatt gcgagtcgat aatcgacatt tatttacgat tatttaccga cctggtgacc
    20401 aataacacca ccgacagctg caccacctaa tgtacccaac gtactgccat cggtcagtac
    20461 tgcaccgcct aatgcccctg cacccgcgcc gattgcggtg ttgcggtccc gtttagacca
    20521 gttagaacag gcactcagag acattgccaa agtaattgcc agaacagccg cggtcatttt
    20581 tttgctcgtt acaaacataa tactctctcc tgaatttatg attcacggaa gtaagctctc
    20641 tataactata atagctaaga ataagcctgg tgaaattatt ccgtgaaatc tgctcgcgcg
    20701 cagggttata tcacgcaggt gataattact tcctgttata tcgctgataa taattttata
    20761 tcttgagagt gttaataaca ggtaaatagt cttaattatc aaccaggaat catcttagag
    20821 cggatgattt gccaaactgc aaatcatccg tagagaaggg aaatggttaa atgtcaacga
    20881 cgtgaacggt gagtttactg tgctcaagat gcatcagatc gctggcgcgt attttcgaat
    20941 cggtaatcac gcgattgaat cgctcaacgg gaccgatgga gtatgaatgt acagcaccaa
    21001 atttcgagct gtcagtcagg actatcgctt cgcactcttt ttccagcacg gcattgacca
    21061 catcggtgcg catcatatcg cgaccggtga atccagtttc aggttgccag ccatcaatac
    21121 caataaatgc tttgctgaaa tgtacctgct ggatgcactg gcgtgtcaaa gggccaacca
    21181 tactttcgct ttttttctgg tacacgccac cgagcaaaat gacttcacaa ggcgcgtctt
    21241 tcagcaaatg cgcgatgtag ctgctgaccg tgatgatagt gacatttttc ttctgctccc
    21301 ccagagtccg ggcgagcagg gcattgctgc tgccattttc gataaagatg gtttcgcccg
    21361 gttgaaccag tgacgcggca aactcggcga gttcacgctt cagcgtatag ttgctcatca
    21421 tacgggtttc gacgtcatca ctatcaagcg aaactgcaaa gccatgtgca cggcggaggt
    21481 aactcagttt ttcgagggtg ttgagatcct ggcgaatggt aacttcagaa actccagtgg
    21541 cttttgccag atcggttacg ctaacctgac cctggtcaat gaccatctgt agaatagttt
    21601 gttgtcggga attcatcgct gtaattctta taacgttata atactaaata aaaaaatact
    21661 gacgcgccgg gcatcctcaa ataggaaaac ctgaaggcac gattgttaaa tctcccacgg
    21721 tgtcttcggg atgtctggcg tagattcagc aacctggcgc aagagttctg ctttaagtat
    21781 ctcgagatta tggatcgcag agtggtaatc gcctgccaca aggatatcta acacaatatc
    21841 aatacgttcg gcaacgcgct gggcatcaaa ttcagacatc aaggacatcc ttatagcaaa
    21901 agggggaaaa tgaataatgc aaaaattgcc gctaaaagag aagtgtttaa cagcaacggc
    21961 taattatcat ccaggaatac gatatataat gacgggatat agcgctaagt atatatattc
    22021 atctacttat gcgcgcttca gatagcgttt ataccagcgt tcgaaggcga cggcgggcat
    22081 cggtttggca aacaaaaatc cttgccgctc attgatcccg ttcttggtta aaaaagcatc
    22141 ttcctttgca ctctctacac cttcggcgat cacctgaaga ttcaatgcct gggccacagc
    22201 gacgatcgcc cggaccagtg actgcgagac aggttgtttg tgaatatctc gaacaaaaac
    22261 ctggtcaagt ttgatggcat cgatcggaaa gcgcgccagt tgcgaaagtg aagagtagcc
    22321 ggtaccaaaa tcatccagat gcacttgcgc acctagttgg ctaaattgtt gaataacaga
    22381 cagtgccagt tcatcattct caatcagaca actctctgtc agttcaacat ctatagggca
    22441 gtattcaaaa ttgagttcct ggagaacctg tttcagggcg gtgaaaatgg tttgatcggc
    22501 gagctgacgt gcagaaatat ttaccgccac tcgcaggttt atgcctttat cccgccactt
    22561 tgccacctgg cgtacgacat cgagaatcac ccaacggcct aaaggcacaa ttagccctga
    22621 ctcttcggcg taggaaatga agtccaacgg tggaatcaac ccacgttcag gtgactgcca
    22681 acgtactagt gcttccagac tgcgcacttc gacacgctag gtgattttcg ataaccaact
    22741 gatcgttttc cagtgcttta cgcaagttgg tatccagcca gagatattca aatacccgct
    22801 gattcatttc tggagtaaaa acgcaaaatt gtcctcgtcc gccttccttc gctgtgtaca
    22861 ttgctgtgtc ggcgtgacga ataatagtcg cgctgtccga accatgttcg ggagagagcg
    22921 aaatacctac tgaacagctg gtataaactt caattaaacc aatacgaaag gggaggcgta
    22981 agcgggtcaa aattcgtgat gccattgctt ccagcgcgct ttgtgaggta ttggatgcca
    23041 gtaccagaaa ctcatcgcca cctggacgcg ccaacacctg gtcatgttcg agacagctta
    23101 aaatagccaa tgacacgtcg cgtaataact ggtcaccaaa caaatgccca taggcgtcgt
    23161 tgaccttttt aaaattatcc aaatcaagat acacaacccc aactttattg ttatctgcct
    23221 gattaatagc gtgatcgatt aactcctgca ttgcgttacg attcggcagt ccggtgatac
    23281 tgtcggtatt tgccagaata cgcagtcgct cctgagtgcg gcgctcttcg gtaatgtcgg
    23341 taccggaaca gattaaaaaa atctcgtttt tgccactgcc gctgtggaca aatttattgc
    23401 gaaacagaaa cagccgctgg cctttacgtg ttggtatcca cagttcgact tcatatgcat
    23461 tgccgcttcg aaaaaataca cggttattgc gcctggatgc cgcagcttca cgacggctca
    23521 taaacagttt aaacacgctt tacccaatga cgtcgtgttc tttcaaccct gtgtaatctt
    23581 cacataaccg attgaagcgt tggatattcc cccggctgtc gagaatcact attgcggaat
    23641 gtgcttcaga aactacctgc tcagcaaagg aaagcccgtg tgataaatca cgagcaaccg
    23701 ccggtgtgtc atgccatgca gacgcgttgc cagcccattc ccgtttattg atttttcgtc
    23761 ctaccagatg cacagaaaga tcgctttcat cgacggtcag actcatcatc aggctggagg
    23821 tgatgaccgt catttccctg atgcgagcag cctgttcggc agataactgc aatgtacgat
    23881 caggttctgt ggttttggtg gtagaaaagc gcaaagcatc gctgctttcc gtcaaccgcc
    23941 agtatggatt gtgcgatccg agaaagttgt acaacgttgt ggactcccta acggttttca
    24001 tgatgcatat cctccggtta acagcggagt ccgcggttaa ataaaaggaa caacatgact
    24061 tacatgaaat taacggcggc taaacgcatt gtttttttat atatttattt gtaatccagt
    24121 tttggaaaaa cgccagtttt caaacgaaag tcagttaaaa aatctgcctg gatataacga
    24181 aggtagagcg gggaaataaa cggcccatcc atgaggaatg ggccgtgaaa ggagattacg
    24241 cgaccgggcg cgcaataatg ctgcgggttt ccatgcggac ttcggcaatg gtgacgtcaa
    24301 taacgtcagt tactttgtaa accgtttcac ctttaatttg tacagtgccg ttttcctggc
    24361 tgcaaaccag ttcatcgcgc acagcgtgta agaaaggtgc cggaataaag gcgatagcgc
    24421 cgttatcaac caaacgcaca cgcatgccgc cacggctgat atcgacaatt tccgccgcga
    24481 aacgggtgcc ggtcccggct ttatctttca ggaagcgtgc gtataaccag tcgccaacat
    24541 cacgttctgc catccggttg agacggcgac gttcggccat ttggacagtg atttcatcct
    24601 gcggacgcgt cgcagtttcg cctttgataa ccgctttcag cagacggtgg ttgatcatgt
    24661 cgccatattt acggatcggc gaagtccagg tggcgtatgc ttccagaccg aggccaaagt
    24721 gaggaccggg ttcagagcta atttcagcaa atgactggaa gcgacgaatg cggctgtcga
    24781 ggaaaccagt tggttgcgca tccagttcac gacgcagttt gcagaaaccg tccagcgtga
    24841 gcacttcttc ggcatcgaca tgcagaccgt gcgttttcag caacgctgcc agcgcgtcgg
    24901 cattcgccgg atcaaagccc atatgcacgt tatagatgcc aaaaccgagc ttatcgcgca
    24961 gtacgcgggc cgcacaaatg ttagcggcaa tcatcgcttc ttcgacgata cggttggcaa
    25021 tgcgacgagg ctcggcgacg atatccagca cttcaccttt ttcaccgaga ataaagcggt
    25081 aatccgggcg atctttaaac accagtgcgt ggttatgacg ccactcgccg cggcgttggc
    25141 aaatttgcgc tagcaaacgg acttgttcgg caattgcttc actttcaggc ttccagtcac
    25201 cggtattttc cagccagtca gaaacctggt catacaccag cttcgctttg gattcgatgg
    25261 tggcggcaaa gaattcgata ttatcttcaa tggtgccatc agcggagagc gtcatgcggc
    25321 atgccagtac cgggcggact tcattggcgc gcagtgagca gagatcgtca gaaagctcgc
    25381 gaggcagcat agggatgttg aagccaggca gatagttggt gaatgcgcga attttcgcgg
    25441 ctttgtccag cttgctgcct tcagcaatcc acgcggttgg atcggcaatc gccacaatca
    25501 gctgaagttt gtcatccggc agcgccttag cgaaaagggc gtcatccata tcttcagtgc
    25561 tggcactgtc gatggtgaca aaatccagcg aggtcagatc ttcacgaacc agaccttcat
    25621 cgagcatttc ggtagcgacg ccgtctggtg cttctttttc cagattatgg cgcgcaaggg
    25681 taacccacca cggtacaaag tggtcgtcac caaaagtgat gtattgtgtc agttcagcat
    25741 agaaagaacg atcgcctttc agcggatgac ggcgcatttc ggcaaccgcc cagtcgcctt
    25801 ctttaaactc gtggttcagg ccacgggctg cgcggcaagg aatggcgtct tttaaaagtg
    25861 gatgatcagg aacgatggtc agacggtcat ttttgccctg aactttaccc acgaaacgag
    25921 tcaggaacgg ttcaaccagt tcttctggct ctgcggattc acgttctttt tcactgtgga
    25981 tcaccgcgat aattcggtcg ccatgcatga cttttttcat ctgcggcggc ggaatgaaat
    26041 aacttttttg tgcgtcgact tccaggaagc caaagccttt ttctgtggct tttaccaccc
    26101 cttcagcgcg tggcgtctgg gaatgcagtt gctgtttaag ctgcgctagc agcgggttgt
    26161 cctgaaacat aattgtctat tttggtggcc attagagcgg ctgacagttt tacgcgaatc
    26221 tgtctgacgc ggcaaggtta atatgtctca cccaacgcga tttttaagcg atttatccag
    26281 ccacacagcc cgctccatac cagcagatta ataatctgcg ttgatgattt ttcgtgttcg
    26341 agtaaagggc tgaactgggc ggcgctaaag cgatcgggtg ctcgggtcag ccattgtatc
    26401 gcctgaacgg tagcgcgctc aacagaatga cggtcagccc atcggttaat ttcattttcg
    26461 cccttgcgca gaagggtgct gattttttta tattcatttg atgaatccat acaggtggaa
    26521 aaacaagcag gactgccatt tatacgcgcg cataacaacg ccgcaagctg attatccggc
    26581 tgtgcattga cccgtgtgct gtttaacaac ttttcgagaa gggaaagtga aatagggtgg
    26641 agagagagtg tttcggccag ttggcaaagt tctgcagttg aactttttgt gtgctcttca
    26701 ggtagttttt ccacctcata gctgcttcgc cagcgtattg attcatcagc aaacagtgac
    26761 gcgtcggcgt agttttgtat cgccagcccg ggtaaccagc gtaccggatg cccgagataa
    26821 gcctgaaatg tcgcaattgt ccgcgcctga aagccaataa aaccaatgat ttggttaatg
    26881 atcacacagt cccagacagt aagaccgacg tcattcagat gctggcgcga tcggttgtcg
    26941 ataatagaag gcgagctagc cagttggcgg gcatattgcg tgatttgtgc caaccgatga
    27001 ttactttccc tggaggaatc aggtccgggg agcggcgtaa gtcgcgccga atagtagtta
    27061 cataaccgct ggacgccgca aacctgagca accgtcaggg ccgtcgacaa gcgttcataa
    27121 gcggtgaagg tttgtgaacg ggtcacggta agctggtcag ggaacaactc cactgcaaga
    27181 acgcgcgcag ggaccagcca tgaaaggaag ggggccagcg tctcttcatc aaggatcacg
    27241 tcgagcagaa agggatcgct gacctttgcg gcttccggga ccagaggcag aacgtcatac
    27301 tccgtagtac tggattgcgt ttcatgatac cagtggcttt tgccggtgat gtggcgttgt
    27361 tccatgggcg ctccttggtc gtaaaggaaa tcgttatcct gacgcaaggc gggaagggga
    27421 gaaagacgga tcggcgataa caaatatcag aaaggtataa cagagataac gggcggcaga
    27481 acgccgccca tctttaccaa cagaacgatt atttcagttc gagttcgttc attgcagcga
    27541 tgctgaaacc gccgtcaacg tggactactt caccggagat accggcagag agatcggagc
    27601 acaggaatgc cgcagagtta cccacatctt caatagtaac tgtacggcga atcggggtaa
    27661 cggcttcgca atgagccagc attttgcgga agtctttgat accggaggcc gccagagtac
    27721 ggatcggacc agcagagatg gcgttaacac gcacaccttc cggacccatc gcgttcgcca
    27781 tatagcgcac gttcgcttcc agagacgctt ttgccagacc cataacgttg tagttcggga
    27841 tagcgcgctc agcgccaagg taggaaaggg tcagcagggc agaacccgga ttcagcatgg
    27901 agcggcaagc ttttgccatt gcaacgaagc tgtaggagct gatgtcgtgg gcaattttga
    27961 agccttcacg ggtaacggcg ttaacatagt caccatccag ctgatcgcca ggtgcaaaac
    28021 caatagagtg tacgaaaccg tcaaatttcg gccaaacttt ccccagttca gcgaacatgg
    28081 tgtcgatgct ggcatcttct gcaacatcgc actgcagaac gatgtcagaa cccaattgag
    28141 cggcaaattc ttctacgcgg cctttcagtt tgtcgttctg gtaggtgaat gccagttcag
    28201 ctccttcgcg gtgcatcgcc tgagcgatac cgtaggcgat ggacagtttg ctggcaacac
    28261 cggttaccag aatgcgctta ccggaaagaa aacccatagc tttaatcctt attgttgatg
    28321 cttgttgtgc ctgaaaatca ggcgattcgt cgttttagta aacagtacga acagataaac
    28381 ggttattata atcaacctgg ctgtgagtag ctatagttac caggtccgac cggagcaggc
    28441 tgcggcaggg ggggcgcttt tccccttacc ctaaccctct ccccagaggg gcgaggggac
    28501 catattgtgc aaatattgtt accccagcaa caaacaggct catacagccc ctaacccttt
    28561 tatggcgatg gctgggatgg ttcagacctt gccgaatatt ctccagcact gtctccatgt
    28621 tttcaccaca acagctattc ggctcggtcg gccccctcgc cctttcaggg tgagggtgac
    28681 cggcggttca gttcctgcag aataatctcc agcaccgcct cctcattaca atcaatctcg
    28741 ttattccaga aacgcagcac ggtccagccc tgcgactcaa gccagctagt gcgcctggta
    28801 tcataggcaa ctgccaaatc atgctgccca ccatccagct caacgactac gcgcgccgag
    28861 cagcaagcaa aatcgagaat gtagctcccc accggatgtt gacggcgaaa tttgaaatca
    28921 ccaaaacgtc ggctgcgaag atatcgccag agctttcgtt cctgcaaagt gagattgcgt
    28981 cttaaatcac gcgcatttga ttttatttta tccatcacct cattctgaca agatttaatc
    29041 tcttgtcaga atgaggtgaa taatctggaa ggaggattca gaaaattagc gatctttacg
    29101 ccacgcatcc gctgtcaatg cttcgccaaa gtgcccggca atcagccgtt tggtgagttc
    29161 atgcagcggc gatgccagca catccgcggt gctgcctcgc tcgacaacct cgccctgatg
    29221 catcaccagc acctggtcgc taatgtgctt catcattccg atatgctggg taacataaat
    29281 atacgaaatg ccctgttttt cctgtaactc cagcatcaga ttaatcaact gcgaacgcat
    29341 tgacatatcc agtgaagcga gggcttcatc ggcaataatg acttttgggc gcaatatcag
    29401 cgcgcgcgcc agacccagac gctgtttttg tccgggtgcc aacatatgcg gatagtaact
    29461 gacgtgatcc ggtagcagcc caaccatacg catcgtttca ataatctgtt tgcgacgctg
    29521 ttccggttcc aggtcggtgt tcaggcgcag tggaaaatcc agaatttgtg agatacgttg
    29581 acggggattc aacgaggtcg aaggatcctg aaaaatcatg cgaatacgct gactacggaa
    29641 ggaataatcg ccaaaatgca gcggatgatc gtcaatcaat aactcgccgc tggtaggttc
    29701 tatcattccc gccagcattt ttgccagtgt ggatttaccc gaaccattct cgccaataat
    29761 cgccagtgtc tggccttcac gtagcgtaaa gctcaagggt tttaccgctt ctacggtctg
    29821 acgacgaaac caaccggtcc ggtagcggaa cgttttactt aaattacgca cttcaagcag
    29881 cgtttcgatc atctcactct ttctccatgt tcagcgggaa atgacaggca tagagatgat
    29941 ttttcgcccc cgtcaaacgt ggcgtcacaa tgcattctcg ttgtgcatac gggcaacgtg
    30001 gccccagacg acaaccaatc ggtaactgtt ccagcaatgg gatagcgccg ggtagagtat
    30061 tgaggcgact tttatgcggc atcgcgctgc cgaagtctgg tatcgcgcgg atcagcgcct
    30121 gggtataagg atgatgtggc atcgtcacca actccttact cggcgcggtt tccactgttt
    30181 gaccgcagta aagcacgtta attttatccg cccattggct aagcatttgt aagtcatggc
    30241 tgataagcaa aatagtggta ttgctgtttt ggttgagacg cgtcagcagg cgaaagattt
    30301 gcgcctgggt tgttggctcc attgagtttg tcggttcgtc agcaatcagc agacgcggtt
    30361 gattcgccag tgcaatggct atcatcactt tctgacattc accttcggtc aactcatagg
    30421 gaaaactgcg catcgcatct ttgtgatctt taatccccac gcggtgcagc agttcaatgg
    30481 cacggcgttt gcgccagcca aaacgctgcc accaacggcc tttataggtc caggctggga
    30541 tgttttgcat caactggcga cccacacgtt ctgaagggtc aagacacgac tgcggttcct
    30601 ggaaaatcat cgacacgtta tggccaacca gtttgcgccg ttcgcgtgcg gagagacgca
    30661 gcaaatcgat atcatcaaaa cgcatacggt cagcagtaac acgccagtta tctttattca
    30721 ccccacaaat cgctttcgca atcaaactct tgccggaacc ggattcacca acaagaccgc
    30781 ggatttcacc ttcggttaag gtcatactta cccggtcgac ggctttaacc cactcatcac
    30841 cggttttaaa ttcaatggtc aggttacgaa tatcgagtaa tggcattatt ccacccccgc
    30901 aataatcgca cgacgaacgc catcgccaag gaggttaacc aacaacacgc taatcataat
    30961 tgccgcacct ggcagcatga cagtccacgg ggcgacataa atcagttcca gcgcatcacc
    31021 gagcatcgct ccccattcag gcgaggggag ttgtgcgccg agatcgagaa agcccagcgc
    31081 ggcgatatcg agaattgcca tcgacagtgc gcgggtgatc tcggtgacca gcccggcggt
    31141 gatgtttggc atcacagcaa accagagaat attcagcgtt gatgcgccat ccagacgggc
    31201 ggcgataacg tactcttttt ccagttcgtc atgcaccatg ctgtaaatcg aacgtaccat
    31261 acgcggcagc aatgccagcc agacagcaaa catagcgtgg gacaaactcg gtccggcaaa
    31321 cgctacaacg ataattgcca acagcagcga agggatcgcc agtaaggtat ccagaatatg
    31381 gttaagcacc gctgagcgga ggccgtgtgt cgccccggcg aaggtgccca gtaccaggcc
    31441 acagatcgtc gcggcaagcg tcaccacaaa tgcgccaccc acggttggcg cagctccgct
    31501 cagtaaacgg cttaaaacat cacgtccgag gtcgtcagtc cccaggaaga aagaaacttc
    31561 gccatagcgc gaccatgatg gcggcaataa ttgataaccg agaaattgct ggtcgatgcc
    31621 gtaaggcgca aaccagccgc caaaaataca cagtacagcc agtcccgcgc agccgtacag
    31681 gccgaccatc gcagaggcgt cactataaaa tttgcgccag gcggtacgca gcgtgcccgg
    31741 cgggcgcttt tcgctgtata cgctatcgta aggcatacca ttccttatgt ttcagagggt
    31801 tagccatggc acccaaaata tcagaaatca cgttaacaat aatgaccagt gagccacaca
    31861 ccatcactcc ggcggaaatg gctgcgtaat cctgctggcg gatggcgtta attaaccagc
    31921 gccctaaacc cggccagcta aaaaccattt cggtgatcat cgccagcgtc agcatggtgg
    31981 aaaactgtaa gcccaggcga ggaataaccg gcggtaacgc gttatgcaga acgtggcgac
    32041 gcaaaatagt aaagcgcgac aaaccgcggg tcgccgccgc ttttacatag ttctggtcat
    32101 acacttcaat agtgctgata cgcatcaggc ggatcacttc agttgttggc gcaaccgaca
    32161 gggtgatcac gggcaatatc atatggcgga ttgcgctcat gatcatttca tcccgccatg
    32221 gtgagtccga aagccaggca tcaatcaacg caaaaccggt aatcggtttc acttcgtaga
    32281 gcagatcgaa acgccctgaa acgggcaacc aacctagcgt gagtgaacaa aacagtgtca
    32341 acagaagcgc cagccagaac accgggattg aaaagcccag taaggcgatg gcgttgatca
    32401 agttatcctg ccacttatgg cgcgtaatcc cggcaatcat ccccacgggg atgccaacaa
    32461 tcagcgcaaa accgaaggcg aggatgcaca gttctatcgt cgccgggaag agctctttta
    32521 actgttcggc aattggttga ccgttaatgc tggaaacacc aaaatcccag tggatcaggc
    32581 cattaaacca gaacacccag gcattccaca gtgacgcgcc ttgtaacggc gcgtgtggag
    32641 tgaaatagct caagctgaag ccaacgaagg tcagcaggaa caaggtgaca atcaataaca
    32701 aaatgcggcg taaggtgaag ataatcatgg ttttttcacc tcatcctgtt tctcgcgata
    32761 caccccagca aaggaggcgt taccaaacgg gctaagtacc agacctttga tatcgtaccg
    32821 ataggcctgc aaacgcaatg acgacgccag cggcagaata ggtaattcct gcgccagaat
    32881 actctgcgct tcgtcatagg cttcaatacg cgctgccagc tgctgcgagg agagcgcctt
    32941 acgcaacacg ctgtcgaatt tcggattgca ccagtgggcg aggttggtct gtgaatgaat
    33001 tgccgcgcag ctcagtaacg gacggaagaa actgtccggg tcgttactgt ccgtcgccca
    33061 accggataac gtcagatcat ggctcatatc catcaaccgc gcctcctgaa agcgaccttc
    33121 taccggcaca atcaccactt ttacgccaac ctgcgccata tccgcctgaa tcagttcggc
    33181 agttttcagt ggactggggt tccacgcctg cgaacgtgtg ggcacccaca gtttcagcgt
    33241 taagttttcc agccccaacg ccttcaactg ttcgcgcgat ttcgccggat tgtattcagt
    33301 aattttagcc tcgttgtcat aggcccacga ggcgcgcggt aaaatagagg ccgccgtttc
    33361 agccgtacca taatagatgg attgcatcag gcgctggtta ttaatcgcca gtgccagtgc
    33421 atggcggaca gcgggattat ttagcggcgg tttggcggtg ttaaatgcca gataggcgac
    33481 gttcatccca ggacgcagcg ttaaacgcaa gcgcgggtcg tcacgcaaaa tggatagctg
    33541 gctggcagca ggccaggcca gaacgtcgca ttccccggtc aggagtttcg acagacgtcc
    33601 ggtgccgccg gagcctaaat ccaccactac ctgcggcatt aacggtttac cgcgccagaa
    33661 gtcatcatga cgttgtaggc gaataaattg cccggcgcgg tattccgaca actgatacgg
    33721 accggtgccg accggttgac ggtcgagttg ctcctggcga tcttctttct ctaacttccg
    33781 ggcatattct gccgacatga ccgaagcata atgggttgcg aggtgccaca aaaaagaagc
    33841 atccggctgg gcgagtcgga actcaacggt atgattatcc agtttgcgga cgcttttcac
    33901 gttatcggca aattgcaggc tgtcgaagta ggggaagttg ctgccgttga cgttatgcca
    33961 cgggttgttg cggtcaaaaa ttcgctggaa ggtaaacacc acatcgtcgg cattcatttt
    34021 acgagtggga gtaaaccagt cggttttttg aaacggaaca tcgcgacgca ggtggaagcg
    34081 ataggtcgcg ccgttgtcga gtacttccca gctttcggca agttccggca tcaggcgata
    34141 ggtataggga tcgacatcca gcagtcgatc ataaaactgg gcggcaaggg tatcgacaat
    34201 taacccactg ctcgctttgg atgggttaaa ggtgttgact tgcccgctga cgcaatagac
    34261 aaaaccgctg tcgcggatat cagcatgcgg gggggattca ggcgcggcga ttgcctgacc
    34321 actcacaagt ccagcaatca ccaaaagaga cgataatacc tggcgcataa tattaaggga
    34381 ttttatgtaa agaggctatc ttactaatat ttaatgacat ttgccattac cgtttgtgtt
    34441 caggggtcgt aatgagggcc gtgtggttgg tcgttgaaca ggtggcgtcg ccatctgttc
    34501 aacattcgta cccgtgattt ctctataact ataactcaca gaacaactta gcgaggagca
    34561 gagcggtacg atcgtgagaa acccattaaa gcacgctgca gtgcgtgtca ttgttagccg
    34621 gatgcggcgt gaacgcttta tccggacaac gatactgacc gatcgtctgc aattaaataa
    34681 ttactcatta ccccattgat tcaaaaactc tgcgatctca tcaatgcgta cgggattaat
    34741 ccccgcttca gcagccattt catgttgggc ttcttcgctg atctcttcat tgttcatcaa
    34801 acgggtgagc agtaactgga agtagtgggc cagcggtgtg cgttcagcct ctgcgacagg
    34861 ctctgcgcat tcggtcgcgt actcatcggc aatatcaaaa tattttagcg ggatatcgtg
    34921 gttcattatt tgcccctggg gttaatgcgc taagcataag ggcggatgat agcactcgtg
    34981 atccgggtca tacatctttt tatagttaat ggcgcggcgc gttaccctca ggcaggataa
    35041 tgcgccgcgc atccggctat tacgtttcgc tcaccggcgt tctgtcacct tcgtacagcg
    35101 gcactttgcg atagcggcgg atcagatacc acaaatatgc gccgcctaac gaagcccata
    35161 ccagaccgag tgtcagtgac gttgactcta ggttaaccca cagcacaccc accgtcagcg
    35221 cgccaaccag cggcatcagc aaatagtgga agtgatcctt ccagctttta ttcattcctt
    35281 tacgccgcca gaaatgatta aacaccgaca gattcacgaa ggtaaacgcc accagagcac
    35341 cgaagttaat cagtgctgtc gcggtgacta aatcaaagaa cagcgccgac agcgcgacaa
    35401 tcccgaccat aatgacgttg agtgccggag tccgccattt tgggtgcaca tagccaaaca
    35461 cgcgctccgg aaacacattg tcacgcccca tcacatacag cagacgcgac acgctggcat
    35521 gtgaagccag gccagacgct aatgtgttca caaacgtggt gcagaggaaa atcgactgaa
    35581 agagcttgcc gccgacgtac agcgcaattt caggcagtgc ggcgtccggg tctttaaagc
    35641 ggctgatatc gggaaagaac agctgcataa agaacgacgc cgcgataaag ataacgccgc
    35701 cgtataccgc cgtcaggaag atggctttcg ggatcacgcg tgcggcgtcc ggcgtctctt
    35761 ccgaaagcgt ggtcaccgca tcgaaaccga ggaacgagaa acagacaatc gtcgcaccgg
    35821 taataatcgg gatcaggtgc gcgttctcgc tgataaacgg ctgaagtgac cagacggtgc
    35881 caacgccttc tcctttatgc agtccctgaa ccaccaggaa gataaacacc accatgatgg
    35941 agatttgcac cagtacaaac agggtattga agttagcgac caggttgacg ctcttcagat
    36001 tcgcggcggt taaaatggcg acgaaggtta ccacccacac ccacggcggc acttccggga
    36061 agagggcgga gagatagatt ttcgccaaca aaacgttgat cattggcaaa aagagataat
    36121 ccagcagtga tgaccagccg accataaatc cgacgtgcgg gttaatcgac ttttgcgcgt
    36181 aggtataggc cgaaccggcc tccggaaact ggcgaaccag tttgccgtag ctgatagcgg
    36241 taaacagcac gcccgccagc gccagcaaat aggacgccgg aacgtgaccg tcgctaatgc
    36301 cggagacaat accaaaagta tcaaatacgg tcatcggcgt gagataggcc agacccatca
    36361 ttaccacctg ccacaatttc agtgattttc gcagacgggt tttgccgggt tgcgcagcaa
    36421 tattcagtgg tgaattaata gccataattg ctgcctcccc tgcgtaataa ggtttgcggt
    36481 cgtgtcgacc tgaaatcaaa cctgtttagc aagggaagca aagggggcgg agtccagtca
    36541 gtagcaggag gggcgcatac gcagcctccc tcgccatgcg gggatttaat catcggcatc
    36601 atctcatttc ctcgtcacag tcttaatcgt taacggaagc ccgttgccgc ctgcgctcca
    36661 taacgcgaca tccgggaaat gtctggatta ccagcaaatg tcaggcctgg ctccgctcag
    36721 gccgatgaaa caaccccgca aggggaatta cgcgtttttc aacatccact caatttctgt
    36781 ttctgtgatg aggcgctcaa actgcaacag ctcatcattt ttacaggcgt gatacacatg
    36841 gcaaaaccgt tcgcctaaat agcggcgcag gtgatcattc tcgataaact cacccagggc
    36901 atcgctctgg cgaatcggga agggtaagcc ttcctgttcc agcccgttgc cttcgacttc
    36961 ttcctgcaac ggcagctcgt tatcaaggcc atgcaaaata ccggcaaaaa tcgctgccat
    37021 caccagatat gggttggcat cggcacccgc cacgtgatat tccacgcggt gattatgacg
    37081 gtcaccgcac ggaatacgca gggcgacggt gcggttgtta tggccccacg acgcctgcgt
    37141 cggcacatac attcccggct ggaagcggcg atacgagttc acgtttggtg ccagcaacgc
    37201 catcgacgac ggcatcaggt caatcatccc ggcgagcatc tttttcagca gcggcgaatc
    37261 ttcgccttct gtgtcagaaa gcacattctc gccacgatta ttttgcatac tgatatggat
    37321 atgcatcccg ctgcccgcgt gctcttcata cggcttcgcc ataaaagtgg cgtgcatctt
    37381 atgcttttct gccatcagac gcactaggcg ttttagtgcc agcgcatcat cgcaggcttc
    37441 cagcacgtta tcagtatggt aaaggttgat ttcaaactga cccggcgagg cctcagcgac
    37501 cgcgccatct gccggaatca gctgcaactg cgccagttca tcaatatcat tgagcacgtc
    37561 ggcgaagtgg ttgaggttat caacggagta aacctggctt tgtgtattgc ggtcatcggt
    37621 gccaggcgcg cagggcggtt gcagatcccc ttcagcgtcg cgctggcgat ccagtaaata
    37681 gaactccagc tctaccgcta caaccgggaa caatccgcgc tgg
//
LOCUS       DADLTY010000013        37627 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.013, whole genome shotgun sequence.
ACCESSION   DADLTY010000013 DADLTY010000000
VERSION     DADLTY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.013"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..175)
                     /locus_tag="JN334_000592"
     CDS             complement(<1..175)
                     /locus_tag="JN334_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HAY7436847.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="JN334_000593"
     CDS             complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="JN334_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY7436848.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1229..1525)
                     /gene="fis"
                     /locus_tag="JN334_000594"
     CDS             complement(1229..1525)
                     /gene="fis"
                     /locus_tag="JN334_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HAY7436849.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="JN334_000595"
     CDS             complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="JN334_000595"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAY7436850.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="JN334_000596"
     CDS             complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="JN334_000596"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAY7436851.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3738..5189)
                     /gene="panF"
                     /locus_tag="JN334_000597"
     CDS             complement(3738..5189)
                     /gene="panF"
                     /locus_tag="JN334_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HAY7436852.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5179..5421)
                     /locus_tag="JN334_000598"
     CDS             complement(5179..5421)
                     /locus_tag="JN334_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HAY7436853.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5530..6879)
                     /gene="accC"
                     /locus_tag="JN334_000599"
     CDS             complement(5530..6879)
                     /gene="accC"
                     /locus_tag="JN334_000599"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAY7436854.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6890..7360)
                     /gene="accB"
                     /locus_tag="JN334_000600"
                     /gene_synonym="fabE"
     CDS             complement(6890..7360)
                     /gene="accB"
                     /locus_tag="JN334_000600"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAY7436855.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8338..9312)
                     /locus_tag="JN334_000601"
     CDS             complement(8338..9312)
                     /locus_tag="JN334_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7436856.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9464..11404
                     /gene="csrD"
                     /locus_tag="JN334_000602"
     CDS             9464..11404
                     /gene="csrD"
                     /locus_tag="JN334_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HAY7436857.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11709..12752
                     /locus_tag="JN334_000603"
     CDS             11709..12752
                     /locus_tag="JN334_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAY7436858.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12818..13921
                     /gene="mreC"
                     /locus_tag="JN334_000604"
     CDS             12818..13921
                     /gene="mreC"
                     /locus_tag="JN334_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAY7436859.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13921..14409
                     /gene="mreD"
                     /locus_tag="JN334_000605"
     CDS             13921..14409
                     /gene="mreD"
                     /locus_tag="JN334_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAY7436860.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14418..15011
                     /gene="yhdE"
                     /locus_tag="JN334_000606"
     CDS             14418..15011
                     /gene="yhdE"
                     /locus_tag="JN334_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HAY7436861.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15001..16470
                     /gene="rng"
                     /locus_tag="JN334_000607"
     CDS             15001..16470
                     /gene="rng"
                     /locus_tag="JN334_000607"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HAY7436862.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16538..20338
                     /locus_tag="JN334_000608"
     CDS             16538..20338
                     /locus_tag="JN334_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HAY7436863.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20494..21939
                     /gene="tldD"
                     /locus_tag="JN334_000609"
     CDS             20494..21939
                     /gene="tldD"
                     /locus_tag="JN334_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAY7436864.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="JN334_000610"
     CDS             complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="JN334_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HAY7436865.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23179..23382
                     /locus_tag="JN334_000611"
     CDS             23179..23382
                     /locus_tag="JN334_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HAY7436866.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23390..24322
                     /gene="aaeA"
                     /locus_tag="JN334_000612"
     CDS             23390..24322
                     /gene="aaeA"
                     /locus_tag="JN334_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HAY7436867.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24328..26295
                     /gene="aaeB"
                     /locus_tag="JN334_000613"
     CDS             24328..26295
                     /gene="aaeB"
                     /locus_tag="JN334_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HAY7436868.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26387..26659
                     /locus_tag="JN334_000614"
     CDS             26387..26659
                     /locus_tag="JN334_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436869.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="JN334_000615"
     CDS             complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="JN334_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HAY7436870.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27343..27813)
                     /gene="argR"
                     /locus_tag="JN334_000616"
     CDS             complement(27343..27813)
                     /gene="argR"
                     /locus_tag="JN334_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HAY7436871.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28248..29186
                     /gene="mdh"
                     /locus_tag="JN334_000617"
     CDS             28248..29186
                     /gene="mdh"
                     /locus_tag="JN334_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAY7436872.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29248..30315)
                     /gene="degS"
                     /locus_tag="JN334_000618"
     CDS             complement(29248..30315)
                     /gene="degS"
                     /locus_tag="JN334_000618"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HAY7436873.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="JN334_000619"
                     /pseudo
     CDS             complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="JN334_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31906..32304)
                     /locus_tag="JN334_000620"
     CDS             complement(31906..32304)
                     /locus_tag="JN334_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HAY7436874.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32498..33625
                     /locus_tag="JN334_000621"
     CDS             32498..33625
                     /locus_tag="JN334_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAY7436875.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33844..34272
                     /gene="rplM"
                     /locus_tag="JN334_000622"
     CDS             33844..34272
                     /gene="rplM"
                     /locus_tag="JN334_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAY7436876.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34288..34680
                     /gene="rpsI"
                     /locus_tag="JN334_000623"
     CDS             34288..34680
                     /gene="rpsI"
                     /locus_tag="JN334_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAY7436877.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35075..35713
                     /gene="sspA"
                     /locus_tag="JN334_000624"
     CDS             35075..35713
                     /gene="sspA"
                     /locus_tag="JN334_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAY7436878.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35719..36216
                     /gene="sspB"
                     /locus_tag="JN334_000625"
     CDS             35719..36216
                     /gene="sspB"
                     /locus_tag="JN334_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HAY7436879.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36259..37626)
                     /gene="dcuC"
                     /locus_tag="JN334_000626"
     CDS             complement(36259..37626)
                     /gene="dcuC"
                     /locus_tag="JN334_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY7436880.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9115 a         9414 c         9900 g         9198 t
ORIGIN      
        1 ttctgtcctg aaaattcagt tcgtcacagt taaccagttt tagtggatta actgagttca
       61 tattacggca tttgtctgtt tctgttgtca gcagttcaat ccactgcatc agtaaagtat
      121 caaacagaaa aacgaagaca gcgaaaacga cgagccacca atacttgcga atcattacct
      181 gagcctgtgc atgtttaacc aagtctggca ggaaatatac acgaaatgaa taatctgaaa
      241 actaggctta cacttccttt actttgcaat gagatcgggg tcaacttcgc ttaaccggct
      301 tcgttttgac aggttgccgg tctttctttt tttcactttc gccagttctt ccgcggagta
      361 atgcgacgcg acatccagcc gtcgaagccc cattttgatg tactcgctgt tgatctcaat
      421 accaatgaat tttcgtccgc tggcgatggc tacggcaccg gtagtaaagc taccggcaaa
      481 cgggtcgaga acgatatcgc ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc
      541 tttcggtttt tgcgtcgggt ggttctcata ttcatccatc aaataacgca cgcgcggaaa
      601 atcccagacg ttacccggta ctttttgatg attgtatggc tgtggaggat ttttgcgata
      661 atcgatcaac gcgcgctgcg atccggtttt ggcttctacc agaatagcat caccgttgaa
      721 tgtgtagttc tttgcgtctt tcaccatcat caggatgggt tcgtacatgg agccgtagtg
      781 ttttttcgcc tgtactccag aactgtcata tgaccagacg atgcgacttt tgatggtaaa
      841 aagcttgcgg cactggagat cgataaaggg catgttttcc gtactgttca taatgtacat
      901 gctgccctgc tttttcagaa cgcggtggca ctctgcaatc acttcaaaca gccagtcgat
      961 aaacagatct tctttccagg cttcgatcag accatcaaaa tttttaccga tgttatacgg
     1021 tgggtcggca aagagcagat cgacgctttc agtaggtagc tttttaagtt cggcaaaggc
     1081 atcaccgtga ataatggtct tagcttcatt accaaaccgg gtcggttcac atcctgttct
     1141 catggtcact ccctttgtga cacctataaa aaaggcgctt ccccatgccg agtagcgcct
     1201 ttttaatcaa gcatttagct aacctgaatt agttcatgcc gtattttttc aattttttac
     1261 gcagcgtacc acggttgatg cccatcatca gcgcagcacg ggtctggtta ccacgggtgt
     1321 attgcatcac catgtccaac aggggctgtt ctacttcagc cagtaccagc tcatagaggt
     1381 cattcacatc ctgaccattc agttgagcaa aatagttctt cagtgcctgt ttaaccgagt
     1441 cacgcagggg tttttgggtt acctgatcct gagagttaac ggtagaaacg gtcagtacgt
     1501 cagaatttac gcgttgttcg aacatagttc tgtcagctct ttatttctgt ttacgcaaaa
     1561 ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg catcctcaat ggcgttgaat
     1621 gtgcgccgaa actggtcatt tggagcgtgt tcctggagat accaggaaac gtgtttacgt
     1681 gcaattcggt acccttttgc cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa
     1741 agcaagcgct taacctctgc caaaggcagt gggggcagca actccccagt gtccagataa
     1801 tgctggattt cccgaaagat ccagggtctt ccctgagctg cgcggcctat catcagggca
     1861 tccgcccctg tatagtcgag cacagctctg gctttaagcg ggtcagtaat gtcgccattc
     1921 gcgataaccg gaatggaaac tttctgctta actgcccgaa tactgtcgta ctcagcttct
     1981 ccattgaaca aacaggcgcg tgtacggcca tgaatggtca gagcctgaat gccacagtct
     2041 tcagccagtt gggcaatctc ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc
     2101 ttcagggtaa caggaacgtc cactgcattg acgacctcgg taaggatcga tttaacgaca
     2161 tccgggtact gcaagagggc tgaacctgcg agcttgcgat tcactttttt agccgggcaa
     2221 cccatattga tatcaataat ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc
     2281 atttctttcg gagcgctacc agcaatttgc acggtgcgaa taccgggttc atcaatgtgc
     2341 accatccgta aacgagattt gtcgctttcc caaacctgtg ggttagaaga catcatctcg
     2401 gatactgtca atccggctcc catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg
     2461 ccagccatgg gcgctgcgat caggcgattt ctgagctgat attgtccgat gcgcatgagt
     2521 taagaaatga ccatactgtg actgcaaggc ggcgtatatt acgcattttt tgcacgagat
     2581 gaaaggccaa actttgacca atcctctgag atggatcaaa gaattgcatt taaaatgagc
     2641 gtggtgcgat aattactcat aaaaatcatc atattagaaa atagtgacta aaatttacac
     2701 tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa
     2761 tttgcgtaaa taatcggcaa atttacgtgc cttttgtgag cttgctcgca cttcgccccg
     2821 cgtcacccta cggcgatgcg aaggtcaatt cttacgaccg gtaatacggc accactcttc
     2881 tttttccacg accgggtcca gtgcgaagct atcggcataa gcttcacaaa cgctctctgc
     2941 ctggcttgcc agaataccgg aaaggcccag caaaccgcct gaaactggca ggacgctgat
     3001 taacggtgcc agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt
     3061 catttcttct ggctgatctt tcggtaagta gagttccaga cggtcagaaa cgccattacg
     3121 ttcggcgtta tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt
     3181 tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat
     3241 gactgtttta ccggttaaat cgaggctgtc gagccattgc aggcacagag aggtggttgg
     3301 atgggtaccc gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc
     3361 cggcacatca cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa
     3421 attatccatc cattcgcgct cccagtcttt atcttctagt tgttcgattt tatgcgcgaa
     3481 gcctgcgccg agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc
     3541 agcgtcgaac agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg
     3601 ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat
     3661 cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca
     3721 aggcatggca aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta
     3781 ccgaaacggt ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg
     3841 tggaagccca ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg
     3901 atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc
     3961 ggccacagga aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt
     4021 tctggcggct tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc
     4081 gacatccgct tcagacgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga
     4141 tctttaatga tcgtagcgga actttgcagc agttgggcgt taattgtcga catgatcgca
     4201 gccataggtg cagccagaaa gatcccggca gcaaaaggtg gcagcacttt taccattaac
     4261 gtcgggatca ccagatctgg tacggtgaga tcggggatca ctgcacgacc taacgctccg
     4321 gccaggtgca taccgaacat caggattgcg actataatcg taccgatgat gatcccccgg
     4381 tgtactgctt tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggccaatc
     4441 acgccaaagc acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca
     4501 gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt
     4561 aagccgccag cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca
     4621 agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac
     4681 gcaatgctga taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc
     4741 agcaggcgcg caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa
     4801 ctcaaactcg ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc
     4861 agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt
     4921 gaaagccaga cagcaggaag ctgaatcatc gccagcaata cccagcccag cccgtattta
     4981 taagcagctc ctggcccgcc gataaacgaa ctggcactga tataggtcgc ggtgagagtc
     5041 atcgccagca caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg
     5101 ccggtgctcc gtttacgcat cgcataaacc gagataccga acaccaccac cagataggcg
     5161 accagcggta gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat
     5221 aaatttcacc atcgcccagc acagtccaat aaacagtagc ggcgtcagga tgcaggccat
     5281 ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcgtctac
     5341 taaccaaact gccagataca aaagggtcag ccccagcgcc cagcgggcct ctttatgggc
     5401 ctgaacaaaa cgagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc
     5461 ttatgggggt tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct
     5521 tttgcagtct tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc
     5581 accatgctgg aagttctcgt cattcatgat gcggatctgc agatcaacgt tggttttgat
     5641 accgtcgatg atcagctcct gcagcgcatt cttcatgcgg gcaatcgcca cgtcacggtt
     5701 ttcaccgtag caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc
     5761 cgcgtagata tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat
     5821 tttgcctggg cttggcagga aggtgttcgg atcttcggcg ttgatacgac attccaccgc
     5881 atggccgcga acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg
     5941 cagctgttct ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg
     6001 aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt
     6061 acctgcaccg cgatagccga tatcaacaca cgctttagcg caacgttcgc cgatgtagcg
     6121 acgcagttcc ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg
     6181 ctgcatggag cagtcacgtt ccgccagata gatagcgttg ccctgaccgt cagccagtac
     6241 ctgaatctcg acgtggcgag gattctccag gtatttctcc atgtaaacca tatcgttgct
     6301 gaaagcagct ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc
     6361 gcgcactacg cgcataccgc gaccgccgcc gccgccggag gctttgataa tcaccggata
     6421 accaatgcgt ttagcaatgg cgcggttttt atccatatcg tcgcccagcg ggccgtcaga
     6481 accaggtacg caaggaacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc
     6541 catcaggcga atggtttctg ctttcgggcc gatgaagata aagccggagc gttcaacctg
     6601 ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc
     6661 ggtgatttca gcggcgctga tgattgccgg gatgttcaga taacttttta ctgacggagc
     6721 agggccaata cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgtgct
     6781 ggagtgcaca gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc
     6841 aatctcgccg cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga
     6901 cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg
     6961 taccggattt gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg
     7021 tatcgcccac gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg
     7081 tgcggtagaa agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg
     7141 ctggcgcttc catggaagga acggtcgccg gagcggctgc gttagattga gctggctgct
     7201 gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac
     7261 ggctaatgcg tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt
     7321 caaccagctc gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt
     7381 gtttagtgtg attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct
     7441 gtatgcatag cgataaattc cagcaggccg ggacgccggt ctattttgcc ccagagtgtc
     7501 aatgttagac ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa
     7561 atataccttc gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga
     7621 cgaggtcgca cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg
     7681 gaatataatc aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc
     7741 gcacaattca cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag
     7801 ctgaaaagcc agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa
     7861 ataatgtagg gatgccagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg
     7921 attacccaat tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa
     7981 ccaactgaca attctcagca ttaaataggt tcgagtggtt aatttgtagt caaataaaat
     8041 cagattattc accccatggt accagaaatc agtaacaggt taagcgtagt gtcgcccagg
     8101 caaagcacca taaacctaac aggcgacgga ggcgtatcaa taatggctgt ttagaggagt
     8161 atcaatgcgc atcaatttgt ggtcagtcga aatcatgact ttccagtttg gatgatgttg
     8221 tacaacttcc tgaccaaggt gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa
     8281 ccgattgaat aatgatgtca aaatgagttc cctctcttat tattccttgc taaatggtta
     8341 gttaaccttc accagcgtgc gaccctggat ctggttatta atgatggcct cggcaaagtt
     8401 cggtgcctct gacagagata tctcttttgc cgcctgggta tagaatgatt ccggtaaatc
     8461 ggcgaccagt cgctgccagg cttgtgcgcg gcgttctggt ggcgtcatta ctgaatccac
     8521 cccttgcaaa cggacattac gcagaataaa tggcatgacc gtggttggca gagtaaaacc
     8581 acccgccaga ccacaggccg ccacgcagcc gccgtaattc atttgcgcca gcacttttgc
     8641 cagcactttg tcgccaacag tgtcaattgc cccagcccag acttgttttt ccagaggacg
     8701 ggattcggca aactcatcac gagggagaat accgctggca cctaaacttt tcagatattc
     8761 atgggtactt tcgcgaccgg aaacggcaac gacctgataa cccaacttat gcagcagcgc
     8821 cacggcggta ctgccgacgc caccactggc acccgtcacg acaatctcac cgtcctgcgg
     8881 gcgaacaccg gcatcttcca gcgccatcac acacagcatg gcggtaaaac cggcagtacc
     8941 gataatcatt gctttacgcg cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt
     9001 cactcgcgcc tgctccgcca gcccacccca gtggttttca ccaacgcccc agccagtgag
     9061 taacacctcc tgaccggcat gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa
     9121 atcgatccca ggaatcatcg gaaaattacg gatgattttt cccttaccgg taatcgccag
     9181 cgcatcttta tagttcaggc tcgaccagtg aacatcgacc gtgacatcgc cctccggcag
     9241 gcgacgttcg tccagagtct gtactgatgc gagagttttg ccgtcctgct gttctaaaag
     9301 taacgcctgc ataagtggtc ctcatgtgca tgatggattg gaaaataatt tctgaagact
     9361 atactcgcta atggaaaagc aaagccgatg aagcgcaata aattgcgaga taaatctgat
     9421 ttgctagtat gcccgcttcc tcactatcgg agttaacaca aggatgagat taacgacgaa
     9481 attttcggcc tttgttacgc tgctcaccgg gttaacaatt tttgtgactt tgctgggctg
     9541 ttcgctaagt ttctacaacg ccattcagta taagtttagt catcgcgttc aggcggtggc
     9601 gacggcgatt gatacccacc ttgtgtcgaa tgacttcagc gcattaaggc cacaaattac
     9661 cgaattaatg gtgtcagcag atatcgttcg tgtagacctg ctgcatggtg ataaacaggt
     9721 ttataccctg gccagaaatg gtagttatcg tccggttggc accagcgatc tgtttcgtga
     9781 actgagcgtt ccgttgataa agcatccggg gatgtcgttg cgtctggttt atcaggatcc
     9841 gatgggcaac tatttccatt cgttgatgac caccgcgccg ctcacggggg cgattggctt
     9901 tatcattctt atgctctttc tggcggtacg ctggttacaa cggcaacttg ccgggcaaga
     9961 attgctggaa acccgggcta ctcgtatctt aaacggtgag cgtggctcta atgtgttggg
    10021 aaccatctat gaatggccgc ccagaaccag cagtgcgctg gatacgctgc ttcgtgaaat
    10081 tcagaacgca cgcgaacaac acagccgtct tgatacgctg atccgctctt atgccgcaca
    10141 ggacgtgaaa accggcctca ataaccgact ctttttcgat aatcagttag caacgttact
    10201 ggaagatcag gagaaagtag gtacccacgg gatcgtgatg atgattcgtc tgccggattt
    10261 caatatgttg agcgatacct gggggcacag ccaggttgaa gaacagttct tcactctgac
    10321 gaatctgctg tcgacattta tgatgcgcta ccctggcgca ctgctggcgc gttaccaccg
    10381 cagtgatttt gctgcgctgt taccgcaccg gacgttaaaa gaggcagaga gcatagccag
    10441 tcagttaatc aaagccgtcg ataccttgcc gaacaataaa atgctcgatc gcgacgatat
    10501 gatccacatt ggtatctgtg cctggcgtag tggtcaggat accgagcagg taatggaaca
    10561 tgcagagtct gccacgcgta atgcgggatt gcagggtggc aatagctggg ctatttatga
    10621 tgactcgttg cctgaaaaag gacgcggtaa tgttcgctgg cgtacgctta tcgagcaaat
    10681 gctcagtcgc ggcggcccgc gcctttatca aaaaccggcg gttactcgcg aaggtcaggt
    10741 tcatcatcgc gaactcatgt gccgcatctt cgatggtaat gaagaggtta gctcggcgga
    10801 gtatatgccg atggtcttgc agtttggctt atcggaagag tatgaccgtc tgcaaatcag
    10861 ccgtcttatt ccactattgc gttactggcc agaggaaaat ctggcgattc aggttaccgt
    10921 tgagtcgctg attcgtccgc gttttcagcg ttggctgcgc gatacgttaa tgcaatgtga
    10981 aaaatcacaa cgaaaacgca taattattga acttgcagag gccgatgtag gtcaacatat
    11041 cagtcgttta caacctgtta ttcgtttagt gaatgcttta ggggtacggg tagccgtcaa
    11101 ccaggctggt ttgacgctgg taagcaccag ttggatcaaa gaacttaatg ttgagttact
    11161 caagctccat ccggggctgg tcaggaacat tgagaagcga acggagaacc agctgctggt
    11221 tcaaagcctg gtggaagcct gctccgggac cagcacccac gtttacgcca ccggcgtgcg
    11281 ttcgcgaagc gagtggcaga ccctgattca gcgcggtgtt acaggcgggc aaggggattt
    11341 tttcgcgtcc tcacagccac ttgatactaa cgtgaaaaaa tattcacaaa gatactcggt
    11401 ttaacctgcc gtttaatccg ttttcacgta gaataacgcg cgctgcgtct catgggagtg
    11461 tgcttgtctg ctcgccagat tgttgcagca catatgcaga tgaatgacct tacgcggttg
    11521 caaacaggcg aggaatgctg ctgatgcatt aagcctttct ggactcaggc agagatttgt
    11581 aacaaaggaa acgaactgca ctaattttca ccgtagcaga tgatttttgc gccttgtcgc
    11641 tgctgcgtgt ggttggtaaa gtaagcggat tttcttttcc gccccagctt tcaggattat
    11701 cccttagtat gttgaaaaaa tttcgtggca tgttttccaa tgacttgtcc attgacctgg
    11761 gtactgcgaa taccctcatt tatgtaaaag gacaaggcat cgtattgaat gagccttccg
    11821 tggtggccat tcgtcaggat cgtgccggtt caccgaaaag cgtagctgca gtaggtcatg
    11881 acgcgaagca gatgctgggc cgtacgccgg gcaatattgc tgccattcgc ccaatgaaag
    11941 acggcgttat cgccgacttc ttcgtgactg aaaaaatgct ccagcacttc atcaaacaag
    12001 tgcacagcaa cagctttatg cgtccaagcc cgcgcgttct ggtttgtgta ccggttggcg
    12061 cgacccaggt tgaacgccgc gcaattcgtg aatccgcgca gggcgctggt gcccgtgaag
    12121 tcttcctgat tgaagaaccg atggctgccg caattggtgc tggcctgccg gtttctgaag
    12181 cgaccggttc tatggtggtt gatatcggtg gtggtaccac tgaagttgct gttatctcct
    12241 tgaacggcgt ggtttactcc tcttccgtgc gcattggtgg tgatcgtttc gacgaagcta
    12301 tcatcaacta tgtgcgtcgt aattacggtt ctctgatcgg tgaagccacc gcagaacgta
    12361 tcaagcacga aatcggttcg gcttacccgg gcgatgaagt ccgtgaaatc gaagttcgtg
    12421 gccgtaacct ggcagaaggt gttccacgcg gttttaccct gaactccaac gaaatcctcg
    12481 aagcactgca ggaaccgctg accggtattg tgagcgcggt aatggttgca ctggaacagt
    12541 gcccgccgga actggcttcc gacatctccg agcgcggcat ggtgctcacc ggtggtggcg
    12601 cactgctgcg taaccttgac cgtttgttaa tggaagaaac cggcattcca gtcgttgttg
    12661 ctgaagaccc gctgacctgt gtggcgcgcg gtggcggcaa agcgctggaa atgatcgaca
    12721 tgcacggcgg cgacctgttc agcgaagagt aatcggatgc aggcagggga agcgtctgtt
    12781 taccctgcct ggtctgatac gagaatacgc ataacttatg aagccaattt ttagccgtgg
    12841 cccgtcgcta cagattcgcc ttattctggc ggtgctggtg gcgctcggca ttattattgc
    12901 cgacagccgc ctggggacgt tcagtcaaat ccgtacttat atggataccg ccgtcagtcc
    12961 tttctacttt gtttccaatg ctcctcgtga attgctggat ggcatatcgc agacgctggc
    13021 ctcgcgtgac caactagaac ttgaaaaccg ggcgttacgt caggaactgt tgctgaaaaa
    13081 cagtgaactg ctgatgcttg gacaatacaa acaggagaac gcgcgtctgc gcgagctgct
    13141 gggttccccg ctgcgtcagg atgagcagaa aatggtgact caggttatct ccacggttaa
    13201 cgatccttat agcgatcaag ttgttatcga taaaggtagc gttaatggcg tttatgaagg
    13261 ccagccggtc atcagcgaca aaggtgttgt tggtcaggtg gtggccgtcg ctaaactgac
    13321 cagtcgcgtg ctgctgattt gtgatgcgac ccacgcgctg ccaatccagg tgctgcgcaa
    13381 cgatatccgc gtaattgcag ccggtaacgg ttgtacggat gatttgcagc ttgagcatct
    13441 gccagcgaat acggatattc gtgttggtga tgtgctggtg acctccggtc tgggcggtcg
    13501 tttcccggaa ggctatccgg tcgcggttgt ctcttccgta aaactcgata cccagcgcgc
    13561 ttatactgtg atccaggcgc gtccgactgc aggactgcaa cgtttgcgtt atctgctgct
    13621 gctgtggggc gcggatcgta acggcgctaa cccgatgacg ccggaagagg tgcatcgtgt
    13681 tgctaatgaa cgtctgatgc agatgatgcc gcaggtattg ccttcgccag acgcgatggg
    13741 gccaaagtta cctgaaccgg caacggggat cgctcagccg actccgcagc aaccggcgac
    13801 aggaaatgca gctactgcgc ctgttgcgcc gacacagccc gctgctaatc gctctccaca
    13861 aagggctacg ccgccgcaaa gtggtgctca accgcctgcg cgtgcgccgg gagggcaata
    13921 gtggcgagct atcgtagcca gggacgctgg gtaatctggc tctctttcct cattgcgctg
    13981 ttgctgcaaa tcatgccctg gccggataac ctgattgttt tccggccaaa ctgggtgtta
    14041 cttatcttgt tgtattggat cctggccttg ccccatcgcg taaatgtggg cacaggtttt
    14101 gtgatgggtg ccatactgga tctgatcagc ggctcgatgc ttggcgtacg cgtattggcg
    14161 atgagcatca ttgcttacct ggtggcgctg aaataccagc ttttccgcaa cctcgcatta
    14221 tggcagcagg cgctggtcgt catgttgctt tcgctggtgg tggatattat tgttttctgg
    14281 gcagagtttt tagtgattaa cgtctctttc agaccggaag tgttctggag tagtgtagtc
    14341 aatggggtgc tctggccgtg gattttcttg ctgatgcgta aagtccgtca gcagtttgca
    14401 gtgcaataaa ggtttctatg acttctctgt atttagcttc cggttctccg cgtcgtcagg
    14461 agttacttgc gcaacttggc gtgacctttg aacgtattgt tacgggcatt gaggagcagc
    14521 gtcagccgca ggagagcgcg cagcagtatg ttgtgcgtct ggcacgcgag aaagcacagg
    14581 cgggtgtcgc gcaaacggca caggatctcc cggtgctggg tgcggatatt atcgttatcc
    14641 tgaacggaga agtgctcgag aaaccgcgcg acgcagagca tgcggcgcag atgttgcgca
    14701 aattatcggg tcagacccat caggtgatga cggcagtggc gttggccgac agccagcaca
    14761 ttctcgattg cctggtggtc accgatgtga ctttcagaac gttaacagac gaagacatcg
    14821 cgggctatgt cgccagcggt gaaccgttag ataaagcagg tgcatacggt attcaggggc
    14881 tgggtggctg ttttgtcagg aagataaatg gcagctatca cgccgtagtc ggcttaccgc
    14941 tggttgaaac gtatgaatta ttaagtaatt ttaacgcact gcgtgagaaa agggataaac
    15001 atgacggctg aattgttagt aaacgtaacg ccttcggaaa cgcgagtggc gtatattgat
    15061 ggcggtattc tgcaggaaat tcatattgaa cgtgaggcgc gacgcggaat agtaggcaat
    15121 atctacaagg gtcgtgtaag tcgtgtactt ccgggtatgc aggcggcttt tgtagatatt
    15181 gggctggata aagccgcgtt tcttcatgca tccgacatca tgccgcatac cgaatgtgtg
    15241 gcgggtgaag aacaaaagca attcacggtg cgcgacatct cggaactggt tcgtcagggg
    15301 caagatctga tggtgcaggt ggtgaaagat ccgcttggca ctaaaggtgc gcgcctgacc
    15361 accgatatca cgctgccttc tcgctatctg gtatttatgc caggggcttc tcacgttggg
    15421 gtttcccaac gaattgaaag cgaatcagaa cgtgaacgtc tgaaaaaagt ggtcgcagag
    15481 tattgcgacg agcagggcgg gtttatcatc cgcaccgcag cggaaggggt tggcgaggct
    15541 gaactggcct ccgatgccgc ttatctgaaa cgcgtctgga ccaaagttat ggagcgtaaa
    15601 aagcgcccgc agacccgtta tcagctgtac ggcgaactgg cgctggcgca gcgtgttctg
    15661 cgtgattttg ccgacgccga actggaccgc attcgcgttg actcacgcct gacttacgaa
    15721 gcattgctgg agtttacctc ggagtacatt cccgagatga ccagcaagct ggagcattac
    15781 accggacgcc agccgatttt cgatctcttt gatgtcgaaa acgaaatcca gcgagcgttg
    15841 gaacgcaaag tagaactgaa atccggtggt tatctgatta tcgaccagac cgaagcgatg
    15901 accaccgtgg acatcaatac cggagcgttt gtcggtcatc gcaatctgga cgacactatt
    15961 ttcaatacca atattgaagc gacgcaggct atcgctcgcc agttacggtt gcgtaatctg
    16021 ggcgggatta tcattattga tttcatcgat atgaataatg aagatcaccg ccgccgcgtg
    16081 ctgcattcgc tggagcaggc gttgagcaaa gaccgggtga aaaccagcgt taatggtttt
    16141 tcggcgctgg ggctggtgga gatgacgcgt aaacgcaccc gcgaaagcat tgagcacgta
    16201 ctgtgtaacg aatgcccaac ctgccacggt cgcggaacgg tgaaaaccgt ggaaacggta
    16261 tgctatgaaa tcatgcgcga gattgttcgt gtccaccatg cttacgactc cgaccgtttc
    16321 ctggtctatg cttctccggc agtagccgaa gccttgaaag gcgaagagtc acactcgctg
    16381 gcggaagtgg aaattttcgt tggcaaacag gttaaagtac aaattgaacc gctctataac
    16441 caggagcagt ttgacgtcgt aatgatgtaa acagatgctg ggccgccatc cggcaaaggg
    16501 tttttgagtc acatttttag cagacaagga gtgacgggtg aggcgattgc cggggatttt
    16561 actgcttact ggagccgcgc tcgttgtgat cgctgccctg ctggttagcg gcctgcgtat
    16621 tgctttaccg catcttaacg cctggcgtcc ggaaatcctc aacaaaatag aatccgcgac
    16681 tggcatgccg gtagaagcca gtcagctctc agccagctgg cagaattttg gcccgacgct
    16741 tgaagcacac gacatccgtg cagaactaaa agatggcggc gaattttcgg ttaaacgcgt
    16801 tactctggcg ctggatgtct ggcagagcct gttacatatg cgctggcagt ttcgcgacct
    16861 cactttctgg cagctgcgct ttcgcaccaa cactcctatc accagcggtg gtagcgatga
    16921 cagtctggaa gccagtcaca tcagcgatct gtttcttcgt caatttgacc atttcgatct
    16981 ccgcgacagt gaagtcagtt ttctgacgcc atccggtcag cgcgccgagc tggcgatccc
    17041 acaactcacc tggctgaacg atccacgtcg acaccgtgcg gaaggcctgg taagcctctc
    17101 cagccttacc ggacagcacg gcgtgatgca ggtgcgcatg gatttgcgcg atgatgaggg
    17161 gttgttaagc aatggtcgcg tctggctaca ggcggatgac atcgacctga agccgtggct
    17221 cggtaaatgg atgcaggaca atattgctct ggaaacggca cagttctccc ttgaaggctg
    17281 gatgacgatc gacaaaggcg atgtaaccgg cggtgacgtc tggctgaaac agggcggtgc
    17341 cagctggttg ggcgagaagc aaacgcatac gctgtcggtg gataatctga ccgcgcatat
    17401 tacgcgtgaa aatccgggct ggcagttctc tattcccgat acacggatca cgatggacgg
    17461 caaaccctgg ccgagcggag cattgacgct ggcctggata ccggaacagg acgttggcgg
    17521 caaagacaat aaacgcagtg acgaactccg gattcgcgct agtaatctgg agctggcagg
    17581 cctggagggc atacgcccgc tggccgcgaa actttcacct gcactgggtg atgtttggcg
    17641 ctccacacaa ccgagcggca agattaacac tctggcgctg gatatcccgc ttcaggcggc
    17701 agacaagacc cgttttcagg catcgtggag cgatctggcc tggaagcaat ggaaattatt
    17761 accgggtgcg gaacacttct ccgggacgct ttccggcagc gttgaaaatg gtttgcttac
    17821 cgcgtcgatg aagcaggcaa agatgcctta cgaaacggta ttccgtgcgc cactggaaat
    17881 cgccgacggc caggcaacta taagctggct gaacaatgac aaaggtttcc agctggatgg
    17941 gcgtaatatt gacgttaaag ccaaagccgt ccatgcgcgc ggcggttttc gttacctgca
    18001 acctgctaac gatgaaccct ggctgggtat tctggctggc atcagtaccg atgatggttc
    18061 acaagcctgg cgctatttcc cggaaaactt gatgggtaaa gacctggttg attatttaag
    18121 tggtgcgatt cagggcggtg aagcggataa cgcgacgctg gtttatggtg gcaatccgca
    18181 actcttcccc tataaacaca acgaaggtca gtttgaagtg ctggtgccgc tgcgcaacgc
    18241 gaagtttgcc ttccagccgg actggcctgc attaactaac cttgatattg aactggactt
    18301 tattaacgac ggtttatgga tgaaaaccga tggcgttaat ctgggcggcg tgcgcgcgag
    18361 taatctcacc gcagtgatcc ctgactactc aaaagaaaaa ctgctgattg acgctgacat
    18421 taaaggtccg ggtaaagccg ttggccctta ctttgatgag acaccgctga aagattctct
    18481 gggtgcgacc ctgcaagaac tccagctcga cggcgatgtg aatgctcgct tacatcttga
    18541 tatcccgctg aacggcgaac tggtaaccgc gaaaggtgaa gtgacgctgc gtaataacag
    18601 tctgtttatc aaaccactcg acagcatcct gaaaaatttg agcggtaaat tcagctttat
    18661 caatggcgat ctgcaaagtg aaccactgac agcaagctgg tttaatcagc cgttgaacgt
    18721 ggatttttcc accaaagaag gggcaaaagc ctaccaggtt gcggtgaatc tcaacggtaa
    18781 ctggcaaccg gcgaaaaccg gcgttctgcc tgaagcggtg aacgaagcat tgagtggcag
    18841 cgtggcgtgg gatggtaaag tgggcattga tctgccttat catgctggcg cgacctataa
    18901 cgtagagctg aacggcgatc tgaataatgt gagtagtcac ttaccttcac cgttagccaa
    18961 acctgcgggt gaaccactag cggtaaacgt taaggttgat ggcaatctca acagctttga
    19021 attaaccgga caggctggtg cggataacca tttcaatagc cgctggttgc ttggtcaaaa
    19081 gctgacgctc gatcgtgcta tttgggcggc agacagtaaa acgctcccgc cgttgccgga
    19141 acaaagtggc gttgaactta atatgccgcc gatgaatggt gccgagtggc tggccctgtt
    19201 ccagaaaggc gctgcggaga gtgtcggtgg tgcagcgagt ttcccacaac acataacgtt
    19261 acgtacgcct atgttgtcac tgggaaatca gcaatggaat aacctgagta ttgtttcgca
    19321 accgacggca aatggcaccc tggttgaggc gcaagggcgt gaaatcaacg ccacgctagc
    19381 gatgcgtaat aacgcgccgt ggctggcgaa tatcaaatat ctttattaca acccgagcgt
    19441 ggcgaaaact cgtggtgatt caacgccgtc atcacctttc ccgacaacgg agcgcattaa
    19501 cttccgtggc tggccggacg cacaaatacg atgcacagag tgctggttct gggggcaaaa
    19561 attcggtcgc attgacagtg atatcaccat ttctggcgat acgttaacgc tgaccaatgg
    19621 actgattgat actggtttct cgcggcttac tgccgatggt gaatgggtta ataatccggg
    19681 gaatgaacgt acctcgctga aaggaaaact gcgtgggcag aaaattgatg ccgccgcaga
    19741 attttttggt gtcacgacgc ccatacgcca gtcgtcattt aatgtggatt acgatttaca
    19801 ctggcgcaaa gcaccctggc agccagatga agcgacgttg aatggcatca ttcatactca
    19861 actgggtaaa ggcgaaatta ccgaaatcaa taccggacat gccgggcaat tgctgcgctt
    19921 attgagcgta gatgccctga tgcgtaagct gcgttttgat ttcagagaca cttttggcga
    19981 agggttctat tttgactcca ttcgcagcac cgcgtggatt aaagacggcg ttatgcacac
    20041 cgacgacacg ctggtggatg gcctggaggc ggatatcgcc atgaaagggt cggtaaatct
    20101 ggtacgccgc gacctgaata tggaagcggt tgtcgcacca gagatttctg cgacggtggg
    20161 cgtggctgcg gcttttgcgg ttaaccccat tgttggcgcg gcagtgtttg ccgccagtaa
    20221 agtgctgggg ccgctgtgga gcaaagtctc cattttgcgc tatcacattt cgggtccgct
    20281 ggacgatccg caaatcaacg aagtgttgcg ccaaccgcgt aaagaaaaag cgcaatgatt
    20341 tgacgagggc gcgtaattgc cccaatctca tatgataatc gttgccaaag gccaacgagc
    20401 cggaacataa ctgtaggccg gatagggcgt tcacgccgca tccggcagcc gtaaaaaatc
    20461 ctctactgca gtaactaacg agtagcaaaa acgatgagtc tgaacctggt aagtgaacaa
    20521 ttgctagcgg cgaacggcct gaaacatcag gacttgttcg cgatcctcgg tcaactggcc
    20581 gaacgtcgcc ttgattatgg cgatctctat tttcagtcga gctatcacga atcctgggtt
    20641 ttagaagacc gcattattaa agatggttct tacaacatcg atcagggcgt tggcgtgcgt
    20701 gcaatcagcg gtgaaaaaac cggatttgct tacgctgacc aaatcagcct gctggcgctg
    20761 gaacagagtg cgcaagcggc gcgcaccatc gtccgtgata gtggtgatgg caaagtacag
    20821 acgctgggcg cggtagagca tagcccgttg tatacctcgg tagatccgct gcaaagcatg
    20881 agccgtgaag agaagctgga tatcctgcgt cgcgtcgata aggttgctcg cgaagcggac
    20941 aagcgcgtac aggaagtgac tgccagcctc agtggtgtct atgaattaat tctggttgcg
    21001 gccaccgacg gcacgctagc ggcggatgtc cgtccgctgg tgcgtctttc cgtgagcgtt
    21061 ctggtcgaag aagatggcaa acgcgaacgc ggtgccagtg gcggcggcgg tcgttttggt
    21121 tatgagttct tccttgccga tcttgacggc gaagtccgtg cggatgcatg ggcaaaagaa
    21181 gcagtgcgta tggcgctggt caatctttct gccgttgctg caccagcggg caccatgccg
    21241 gtagtacttg gcgcaggttg gccgggcgtg ctgttgcatg aagcggtagg tcacggtctg
    21301 gaaggcgact tcaaccgccg tggcacttca gtatttagtg gacaggtcgg ggagctggtg
    21361 gcttcagaac tgtgtaccgt ggttgatgac ggcacgatgg tcgatcgccg tggttcggtg
    21421 gcgattgatg atgaaggtac gccaggccag tacaacgtgc tgattgagaa cggcattctg
    21481 aaaggctaca tgcaggataa actcaacgcg cgtttgatgg ggatgacgcc gactggcaac
    21541 ggtcgccgtg aatcctacgc ccatctgccc atgccgcgta tgaccaacac ctatatgctg
    21601 ccgggtaaat cgaccccgca agaaattatt gaatccgttg agtacggtat ctatgcaccg
    21661 aactttggtg gcggtcaggt ggatatcacc tccggcaaat tcgttttctc cacttcagaa
    21721 gcatatctga ttgaaaacgg taaagtaacg aagccggtga aaggcgcaac gttgattggt
    21781 tccggtatcg aaaccatgca gcagatttcg atggttggca acgacctgaa actggataac
    21841 ggcgtgggtg tctgcggtaa agaagggcaa agtttgccgg ttggcgtggg ccagccaacg
    21901 ttgaaagtcg ataacctgac tgttggcggt actgcgtaat aatttcatta ttttcagaag
    21961 gataattaat ctttctacgt gcacgaacgg tcccctcgct cctctggggt tagggtgagg
    22021 ggaacccgtt ggcacaggtt tgtagaacgt aacagtacaa tatgaattac ttctctttcc
    22081 cgcgcccgtg catctcctga aacaatttac cgacctcaac aaaataatcc gtcagcgagt
    22141 tgatcacgac ctgtaccttc agcggcagct tatctttttc ggtatataac gcataaaccg
    22201 ggcgtggatc tgactgataa cgcggcagca ggatctccag ctccccacga ttgatctcgt
    22261 tgatcaccca catcagcggc acgtaggcga tcccggcacc cgccgttagc cagcgcacca
    22321 gcgtcatcgg atcattagtc acaaatctcc cttgcgggat caggcgagtc gagatccctt
    22381 ccggtgcaat cagttcaaat tcattgtcgg gccgcacgct gtattcaagc catgaatggc
    22441 tacttaaatc ggcgggtttt tccggtatgc cgtattgtgt gagatagctt ttcgcggcgc
    22501 acaccaccat tggcatcgcg ccaagacggc gggaaaacag gctggaatcc tgcaacgcgc
    22561 cgacgcggat caccacatcc agaccgtcgg caatcaggtc gggggctgga attccggtaa
    22621 ccagattggc gctcaaacct gggtattctt tcagcatttt ggctgtcagc ccggcgagaa
    22681 cattttgtgc catagttgaa gaacagccaa tgcgtagcgt cccgatgggg gtgttattga
    22741 aggcatacag ttgctcatga acatcctgca cttcatgaag catacgacgg cagccctggt
    22801 agtaaattct accggcttcg gtcaggccaa tgctgcgtgt gctacggttt aacagcttta
    22861 cctgcaactc atcttccagt tttgacaccg tctgactgat ggacgaaacg ctcatctgta
    22921 gctgtctggc ggcggcggta aaagagccaa attcaactac tttggcaaac accgacatgc
    22981 gttttagtcg ttccattatt cactctgact taaaagtgat ttagatcaca taatatagat
    23041 aacagcataa cagttacgct aatatattaa atatcaatct acagcaatgt tgctctcgcc
    23101 cggctttcca tgcccttctc tgcggcgaca gatgctgaaa ataataacgc ctgctctctc
    23161 tatacaacca aggtcaacat gagtctgttt cccgttatcg tggtgtttgg gctgtccttc
    23221 ccaccgatat tttttgaatt gcttttatca ctggcgattt tctggctggt gcgccgggta
    23281 cttgtgccaa caggtatcta cgactttgtc tggcatccgg cgttgttcaa caccgcgctc
    23341 tattgctgct tgttttattt gatatcgcga ctgttcgttt gaggttgaag tgaaaacact
    23401 aataagaaaa ttctcccgta cggccatcac ggtcgtatta gtcattctgg ccttcatcgc
    23461 aatttttaat gcctgggtct attacaccga atccccctgg acgcgtgacg cgcgctttag
    23521 cgctgacgtc gttgcgatcg cgccggacgt ttctggactc attacccagg tgaatgttca
    23581 tgataaccag ctggtgaaaa aaggacagat actgttcacc atcgaccagc cgcgctatca
    23641 aaaggcgctt gaggaagcgc aagccgatgt tgcttattat caggtactgg cacaggagaa
    23701 acgccaggag gccggacgtc gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat
    23761 cgaccaggct aacaacgtac tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac
    23821 ccgcgatctg gcaaaactgg atcttgaacg cacggtgatc cgagcgccag cagatggctg
    23881 ggtgaccaac ctcaacgtct ataccggtga gtttattact cgaggatcaa cggcggttgc
    23941 gctggtgaaa cagaactcct tctatgtact ggcctatatg gaagaaacta agctggaagg
    24001 ggtgcgtccg gggtatcgtg cagagatcac gccgcttggc agtaacaaag tgctgaaagg
    24061 gactgttgat agtgttgccg ctggggtcac caacgccagc agcacgcgtg acgacaaagg
    24121 gatggcgacc atcgactctg accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg
    24181 tattcgtctc gacaaccagc aagagaacat ctggcctgcg ggcaccactg ctacagtggt
    24241 ggtcactggc aaacaagatc gcgacgaaag ccaggattcg ttcttccgta aaatggccca
    24301 tcgcctgcgt gagtttggtt aatcacgatg ggtattttct ccattgctaa ccaacatatt
    24361 cgctttgcgg taaaactggc gaccgccatt gtactggcgc tgtttgttgg ctttcacttc
    24421 cagctggaaa cgccacgctg ggcggtactg acagcggcga ttgttgccgc cggtccggcc
    24481 tttgctgcgg gaggtgaacc gtattctggc gctattcgct atcgtggctt tttgcgcatc
    24541 atcggcacat ttattggctg tattgccgga ctggtgatca tcattgcgat gatccgcgca
    24601 ccattattga tgattctggt gtgctgtatc tgggccggtt tttgtacctg gatatcctcg
    24661 ctggtacgaa tagaaaactc gtacgcgtgg gggctggccg gttataccgc gctgatcatt
    24721 gtgatcacca ttcagccgga accattgctt acgccgcagt ttgccgtcga acgttgtagc
    24781 gagatcgtta tcggtattgt gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg
    24841 atcaaacaag aagtagatcg agagctggaa agtttgctgg tcgcgcaata tcaattaatg
    24901 caactctgta tcaagcatgg cgatggtgaa gttgtcgata aagcctgggg cgacctggtg
    24961 cgacgcacca cggcactaca aggcatgcgc agcaacctga atatggaatc ttcccgctgg
    25021 gcgcgggcca atcgacgttt aaaagcgatc aatacgctat cgctgacgct gattacccaa
    25081 tcctgcgaaa cttatcttat tcagaatacg cgcccggaat tgatcactga tactttccgc
    25141 gaattttttg acacgccggt agaaaccgcg caggacgtcc acaagcagct caaacgcctg
    25201 cggagagtta tcgcctggac tggggaacgg gaaacgcctg tcaccattta tagctgggtc
    25261 gcggcggcaa cgcgttatca gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac
    25321 gccaccgaag aagagatcct gcaaggcgaa ccggaagtaa aagtagagtc agccgaacgt
    25381 catcatgcaa tggttaactt ctggcgaacc acactttcct gcattctggg cacgcttttc
    25441 tggctgtgga cgggctggac ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg
    25501 tcactggcaa tgcgtttgcc gaatccacgc atggtggcga tcgactttat ctatgggacg
    25561 ctggccgcgc tgccgttagg gctgctctac tttttggtga ttatccctaa tacccaacag
    25621 agcatgttgc tgctgtgtat tagcctggca gtgctgggat tcttcctcgg tatagaagta
    25681 cagaaacggc gactgggctc gatgggggca ctggccagca ccataaatat tatcgtgctg
    25741 gataacccga tgactttcca tttcagtcag tttctcgaca gcgcattagg acaaatcgtc
    25801 ggctgtgtgc tcgcgttcac cgttattttg ctggtgcggg ataaatcgcg cgacaggact
    25861 ggacgtgtac tgcttaatca gtttgtttct gccgctgttt ccgcgatgac taccaatgtg
    25921 gcacgtcgta aagagaacca cctcccggca ctttatcagc agctgttttt gctgatgaat
    25981 aagttcccag gggatttgcc gaaatttcgc ctggcgctga cgatgattat cgcgcaccag
    26041 cgcctgcgtg atgcgccgat cccggttaac gaggatttat cggcgtttca ccgacaaatg
    26101 cgccgcacag cagaccatgt gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt
    26161 ggccagttgc tggaagaact ggaaatctac caggaaaagc tacgcatctg gcaagcgcca
    26221 ccgcaggtga cggaaccggt tcatcggctg gcggggatgc tccataagta tcaacatgcg
    26281 ttgaccgata gttaagtcaa aaccgacgcc aaaagcgtcg gttttttcat ggctatactt
    26341 agcgatgcac ggcagaactc gccgcgaaac gtgacggtgg caacagatga atatttatgc
    26401 ctttgatttt gatgagattg agagtcagga ggatttttat cgtgacttta gccaaacctt
    26461 tggtctggct aaagataagg tacgcgatct cgactcacta tgggatgtgt taatgaacga
    26521 tgtcctgccg ctaccacttg agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg
    26581 ttttggcgcg ttaatattgc tgtttgatga ggcagaggaa gagctggaag ggcatttgcg
    26641 ttttaatgtt cgtcattagc gcaaaaaaaa gcccacgaac cgggggcaat atcgtcggac
    26701 aaaacgatga gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga
    26761 gcttcagtaa tctggtaggc cgttgcgcct ttctcttccg cttttttgtt cagcatttca
    26821 cgtatatcca ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga
    26881 ttttgtgctt gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa
    26941 agtacgctta atgcagctac agtggttttg attttcatga tttttacctc gacataatct
    27001 tttagctggg cctttgtttc gtgacccata tcacaaaatc aagtatacac taattattga
    27061 actaattaat accacactaa ctgtttcagt tgatactaag tgttaaaaat gtgaatttta
    27121 ataacaaaaa agtataaaaa acacggttat ttattattaa ttatagtatt ttcaattaga
    27181 taatgaattt tgcataatgt gcttttttta ccgtatgtat tcattttgtg aatgatgtgt
    27241 cgcggtaaaa cgcactattc gttaaatgta tcgcaggaga aagaagggga atgagaggga
    27301 taagcaacat tttccccgcc gccagaagcg acggggcaga gattaaagct cctggtcgaa
    27361 cagctctaaa atcgcttcgt acaggtcttt gacggtgaaa ccgttagcag gggtggtaaa
    27421 gatggtgtca tcgccagcga tggtgcccag aataccttct gctttgccca gtgagtccag
    27481 caggcgagca attaactgcg ccgcgccagg gctggtatga atcacgacaa ctgcatcgtt
    27541 gtagtcgata tccagcacca gattcttcaa tggactggag gtggttggta cacccagttc
    27601 agctggcagg cagtaaacca tttccatttt ggcattgcgt gtacgtacag caccaaactt
    27661 ggtcaacatc cgcgagactt tagactgatt aatattgtca aagccttgct cctgcaacgc
    27721 ggcgacgatt tcgccctggg agctaaattt ctcttcttta agtaatgctt taaatgcttt
    27781 aactagttct tcttgcttag ccgagcttcg cataagtcac ccgatatggt ggttgataca
    27841 acattattgt gcatacagat gaatttttat gcaaacagtc agccctgaag aaggctgaaa
    27901 taatgttatg aaagagcggg attttatcaa atttcgttat tgagaaacat gcctgcgtca
    27961 cggcatgcaa attctgttta aaagtaaatt aattgttatc aaattgatgt tgttttggct
    28021 gaacggtagg gtatattgtc accacctgtt ggaatgttgc gctaatgcat aagcgactgt
    28081 taattacgta agttaggttc ctgattacgg caattaaatg cataaacgct aaacttgcgt
    28141 gactacacat tcttgagatg tggtcattgt aaacggcaat tttgtggatt aaggtcgcgg
    28201 cagcggagca acatatctta gtttatcaat ataataagga gtttaggatg aaagtcgcag
    28261 tcctcggcgc tgctggcggt attggccagg cgcttgcact actgttaaaa acccaactgc
    28321 cttcaggttc agaactctct ctgtatgata tcgctccagt gactcccggt gtggccgtcg
    28381 atctgagcca tatccctact gctgtgaaaa tcaaaggttt ttctggtgaa gatgcgactc
    28441 cggcgctgga aggcgcagat gtcgttctta tctctgcagg cgtagcgcgt aaaccgggta
    28501 tggatcgttc cgacctgttt aacgttaacg ccggcatcgt gaaaaacctg gtacagcaag
    28561 ttgcgaaaaa ctgcccgaaa gcgtgcattg gtattatcac taacccggtt aacaccacag
    28621 ttgcaattgc tgctgaagtg ctgaaaaaag ccggtgttta tgacaaaaac aaactgttcg
    28681 gcgttaccac gctggatatc attcgttcca acacctttgt tgcggaactg aaaggcaaac
    28741 agccaggcga agttgaagtg ccggttattg gcggtcactc tggtgttacc attctgccgc
    28801 tgctgtcaca ggttcctggc gttagtttta ccgagcagga agtggctgat ctgaccaaac
    28861 gtatccagaa cgcgggtact gaggtggttg aagcgaaagc cggtggcggg tctgcaaccc
    28921 tgtctatggg ccaggcagct gcacgttttg gtctgtctct ggttcgtgca ctgcagggcg
    28981 aacaaggcgt tgtcgaatgt gcctacgttg aaggcgacgg tcagtacgcc cgtttcttct
    29041 ctcaaccgct gctgctgggt aaaaacggcg tggaagagcg taaatctatc ggtaccctga
    29101 gcgcatttga acagaacgcg ctggaaggta tgctggatac gctcaagaaa gatatcgccc
    29161 tgggcaaaga gttcgttaat aagtaattga ttagcgaata ataaaaaacc ggagcacaga
    29221 ctccggtttt tgttttgagc actcgactta attggttgcc ggatattcct gaatggtgac
    29281 ctgcagcgtt aactgcttat catcacgcat cactacaacc gggatcaccg aaccagggcg
    29341 aatttccgcc acctgatcca tcgtctccag agcagagatg gccggtttgt tatccaccga
    29401 aataatcaga tcgttgacct gaatacccgc attcgccgct gggccgtcag gtgacacttc
    29461 attaaccacg atcccttgca gttgatctat accaccgctc tgcgcgtgca gtggtgcgat
    29521 ctcgcgtccg ccgataccaa tgtagccgcg gatcacgcga ccatcgcgga tcagcttatc
    29581 cataattttg gttgctaact ggaaaggaat cgcaaagccg ataccttccg gcgtttcgcc
    29641 atcgttactc ttatcaaacg acagcgtgtt aatgcccatc agttctccca gcgagttcac
    29701 cagcgcgccg ccagagttac cgtggttaat ggaagcatcg gtttgtagga agttttgccg
    29761 cccggtcggg ttcagaccga ttcgacccgt ggcactaata atcccttggg taatggtctg
    29821 cccgaggttg tacgggttac cgatcgccag tactacgtcg ccaatgtgcg gtacgcgacg
    29881 tgcattaatt ggaatggtag gtaaaccgcc agtggcatta attttaagta ccgccagatc
    29941 ggttagagag tcagatccca ccagcaatgc ttcaaataca cgtccatcct gtaaggcgac
    30001 gatgatctga tcggcgtcgt tgatgacgtg tttattggtg atgatataac cgcgttgatc
    30061 catgattaca ccggatccca gggtgcggat ctcaagctgg ttgtgagagt tggtgttcaa
    30121 accacggttg taaacgttaa ccaccgctgg cgcggcgcgg cgaaccgcca gattatagct
    30181 ggcaggcgtc tcatcggtac tgtcaaattg cggagtggaa agcgggttaa ggctgcgcag
    30241 cgaaggcatg gcaaccagca gaatagcgcc gacaattaat ccaatcgcaa cggaacgtaa
    30301 gagcttcaca aacatgatgg aggcgtcatt aaaaaaggga acggcagcag cataccacga
    30361 gttaaccgga catcacacgt cagcctgatg cccggtttac gacattaacg catcagcaga
    30421 tagatgcttt cattgccgcg tacaatttgc agggcgatga tggccggttt tgccgccagc
    30481 actttacgca tttcagcaat cgagttcacc cgatcgcggt tgacgccaat gatcacatcg
    30541 tctttttgca agccagcctg agcagctggg cttcctttga caacttcatc tatcttaata
    30601 cctttgccgc catcttttag ctgaccatcg ctcaacgttg caccttccag cgctggccga
    30661 cgaagaggtg ctggtatcaa gcgtcacttc tacttccagt ggtttgccgt tacgcagcag
    30721 gccaagcttc actttcgtgc ccggctctgt agtcgcgata cgagagcgca actcagcaaa
    30781 gctattcagc ggtttgccgt tgaggctggt aataatatcg cccgctttga cgcccgcttt
    30841 cgctgagcca gaacttggca acacttcgct gacaaacgcg ccacgctgca cgtcaaggtt
    30901 gaaggctttg gcgatatcgg cactcatctc ggtgcctttg atgcctaaca aaccgcgttt
    30961 gatttcacca aagtcgataa gctgctgcgc cagtgttcgc gccatattac tggggatggc
    31021 aaatccaatc ccgacgctcc cgccgccagg cgcaaggatt gcagtgttga tgccaattaa
    31081 ctcaccgtta aggtttaata gtgcaccgcc ggagttaccg cggttaatgg aagcatctgt
    31141 ctggataaag ttttccagac cttcaagatt caacccgctg cggcctaatg cggaaacaat
    31201 gccagaggtg gcggtttgcc caaggccaaa tgggttaccg accgctacgg caaaatcacc
    31261 gacgcgcaat ttatcggagt cggcaatagc gatttgcgtt aatttgctcg ggttttgaat
    31321 ttgtaacagg gcgatatcgc tctggtcatc gctaccaatc agttttgcat caaactcgcg
    31381 cccatcattg agctgaatac tgattttctg tgcctgatta atcacatggt tgttggtcag
    31441 cacatagcct ttactggcgt tgatgatgac accggaacct aaaccttcga agggttgtgc
    31501 aggttgatcc ggtaaatcat caccaaaaaa ctttttgaat tcttccggga ttttctgtcc
    31561 ctgactggcc gttccttcca cccgtacgct caccactgcc ggaagcactt tttccagcat
    31621 tggagccaga ctggggagag gggcctgatc ggcaacctgg cctggaatcg acgcgacggc
    31681 ctgaaatgac gccgagagag ttaacccggc acttaacgct aatgcactca acagctgggt
    31741 ttgttttttc attattcctg ctctcgtacc tgaatgataa gaaaagagat tcaaaacagt
    31801 ttgatgttat tgaattttca gacggctaac aatgagacac cagattaaat gatagtcggg
    31861 gagggagaga agaggagggc gcaatggctg cgcccgaaaa ataaattagt cgcgcttcgc
    31921 gccagtacgc agcaggccgg atgcgccttc agaatagtcg cgaggcacct gcaccggtgc
    31981 ctgatcgttg ctggcttcag actctgccag acgattacgg aacgggtttg cttcagcaga
    32041 cagttccggc agcaggctgc tggagctttt tgccatgtgc tgatacagct ggcgatagtc
    32101 gtgcgccatg gtatccagta attccgcgct gcgggcaaag tggctaacca gctcttcgcg
    32161 atactcgtcc agttcagctt tattcttttc cagttcgtac tgcaacgcct gttgctggcg
    32221 tagtttacga ttaccaaaac gcatggccac agcaccaata atgatgccga cgactaaccc
    32281 aattagcgca tattcccagg tcatgaacat ctcccgttgt cttttgtttc cgtagggtgt
    32341 tggcttcagg ctccatgcct gcggctgatt atgccactat aaccgttaat tccacagaag
    32401 tggaatcccg actgcatatc gcgtagtgta gaacggcctt tttttcgtca accgtgaaca
    32461 acggcgcacc gattattcaa ggaataacaa taagatcatg caaagcgtta ccccaacatc
    32521 gcaatacctg aaggcgctca atgaaggcag ccatcaaccc gacgacgttc aaaaagaggc
    32581 cgtcagccgc ctggaaatta tttatcaaga actcatcaat agcaggccac cagcccccag
    32641 gacgagtggg ctaatggcgc gggtcggtaa gctgtggggt aaacgcgaag acacaaagca
    32701 tacgccagtg cgtggcttat atatgtgggg cggtgtagga cgcgggaaaa cctggctgat
    32761 ggaccttttc tatcaaagcc tgccgggaga gcggaaacag cgcctgcact ttcaccgttt
    32821 tatgctgcgg gtgcacgaag agctaactgc cttacagggg cagaccgatc cgctggaaat
    32881 tattgccgat cgctttaaag ccgaaactga cgtgctctgt tttgacgaat tttttgtttc
    32941 tgatattacc gacgccatgc tacttggcgg tctgatgaaa gccctgttca cccgcggtat
    33001 taccctggta gcgacgtcaa atattccgcc ggacgaactt tatcgaaatg gcctgcaacg
    33061 tgcgcgtttt ctgcctgcaa tcgatgccat taaacagcat tgtgatgtaa tgaacgtgga
    33121 cgctggtgtt gattatcgac tgcgtacact cactcaggcg catctgtggc tttcgccact
    33181 caacgatgaa acccgggcgc agatggataa actatggttg gcgctggcgg gggtgaaacg
    33241 agaaaattca ccgacgttag aaatcaacca tcggccattg gcgacaatgg gcgtcgagaa
    33301 ccagacgctg gcggtttctt ttactacgct gtgcgtcgac gcccgcagtc agcatgacta
    33361 tattgcgctc tcacgcctgt ttcacacagt aatgttgttt gatgtgccag tcatgacgcg
    33421 gttgatggag agcgaagcgc ggcgctttat tgcgctggtg gatgagtttt acgagcgcca
    33481 tgtcaaatta gtggtgagtg cagaagtgcc gctgtatgaa atttatcagg gtgagcggct
    33541 gaagtttgag ttccagcgtt gcctgtcacg tctgcaagag atgcaaagcg aagagtatct
    33601 gaagcgcgag catttggcgg gttaaaacct gccacaaatc acaaaaaggg gtcgatcttt
    33661 gaccccgact tctctataat cctgcgaccc cacgttacaa gaaagttttt ttcccaaaac
    33721 tttttgtgtg ctggcatagg ctattcgaag gggtaggttt gccggacttt gtcgtgtgaa
    33781 cctcaacaat tgaagacgtt tgggtgttca ccaacgtgta actatttatt gggtaagctt
    33841 ttaatgaaaa cttttacagc taaaccagaa accgtaaaac gcgactggta tgttgttgac
    33901 gcgaccggta aaactctggg ccgtctggct actgaactgg ctcgtcgcct gcgcggtaag
    33961 cacaaagcgg aatatactcc gcacgtagat accggtgatt acatcatcgt tctgaacgct
    34021 gacaaagttg ctgtaaccgg caacaagcgt actgacaaag tgtactatca ccacaccggc
    34081 cacatcggtg gtatcaaaca agcgaccttt gaagagatga ttgctcgccg tcctgagcgt
    34141 gtgattgaaa tcgcggttaa aggcatgttg ccaaaaggcc cgctgggtcg tgctatgttc
    34201 cgtaaactga aagtttacgc gggtaacgag cacaaccacg cggcacagca accgcaagtt
    34261 cttgacatct aatcgggatt ataggcaatg gctgaaaatc aatactacgg cactggtcgc
    34321 cgcaaaagtt ccgcagctcg cgttttcatc aaaccgggca acggtaaaat cgtaatcaac
    34381 caacgttctc tggaacagta cttcggtcgt gaaactgccc gcatggtagt tcgtcagccg
    34441 ctggaactgg tcgacatggt tgagaaactg gacctgtaca tcaccgttaa aggtggtggt
    34501 atctctggtc aggctggtgc gatccgtcac ggtatcaccc gcgctctgat ggaatacgac
    34561 gagtccctgc gttctgaact gcgtaaagct ggcttcgtta ctcgtgacgc tcgtcaggtt
    34621 gaacgtaaga aagtcggtct gcgtaaagca cgtcgtcgtc cgcagttctc caaacgttaa
    34681 ttggcttctg ctacggcaga aaacaatttg cgaaaaaacc cgcttcggcg ggttttttta
    34741 tggctaaatt ctgaatcagc gtaaaaaatg ggaagttgct ttttgctgcc gtctgaaaga
    34801 caggtaaaac aaaaaccaca tcgccaataa gggtctaagt caactatttc agactaaagc
    34861 gcatctcttt ttccccattt ccggcatcca ctcaccacaa aggtcgcaaa atctggtaaa
    34921 ctatcatcca attttctgcc caaatgtcgg gtattgctca ttttttgttt gattttcgaa
    34981 caaagagagt cgttccttat tgggtaacac aacttctgac tggccacctg gtggctggta
    35041 gcagtaaaaa ttctgactat acctggaggt tttcatggct gtcgctgcca acaaacgttc
    35101 ggtaatgacg ctgttttccg gtcctactga catctatagc catcaggtcc gcattgtgct
    35161 ggctgagaaa ggtgtaagtt tcgagatcga acacgtggaa aaggacaatc cgcctcagga
    35221 tctgattgac ctcaacccga atcagagcgt tccgaccctg gtggatcgtg agctgaccct
    35281 gtgggaatct cgcatcatta tggaatatct ggatgagcgt ttcccgcatc cgccactgat
    35341 gcctgtttac ccggtagctc gcggtgaaag ccgtctgtac atgcatcgca tcgaaaaaga
    35401 ctggtacacg ctgatgaaca ctatcatcaa cggttcagct tctgaagcag atgccgcacg
    35461 taagcaactg cgcgaagaac tgctggcgat tgcgccggtc ttcggtcaga agccgtactt
    35521 cctgagcgat gagttcagcc tggtcgattg ctaccttgct ccgctgctgt ggcgtctgcc
    35581 gcaactgggc atcgagttca gcggcccggg tgcgaaagag ctgaaaggct atatgacccg
    35641 cgtctttgag cgtgactctt tccttgcttc tttaactgaa gcagaacgtg aaatgcgtct
    35701 gggccggagt taatctgtat ggatttgtca cagctaacac cacgtcgtcc ctatctgctg
    35761 cgtgcattct atgagtggtt gctggataac cagctcacgc cgcacctggt ggtggatgtg
    35821 acgctccctg gcgtgcaggt tcctatggaa tatgcgcgtg acgggcaaat cgtactcaac
    35881 attgcgccgc gtgctgtcgg caatctggaa ctggcgaatg atgaggtgcg ctttaacgcg
    35941 cgctttggtg gcattccgcg tcaggtttct gtgccgctgg ctgccgtgct ggctatctac
    36001 gcccgtgaaa atggcgcagg cacgatgttt gagcctgaag ctgcctacga tgaagatacc
    36061 agcatcatga atgatgaaga ggcatcggca gacaacgaaa ccgttatgtc ggttattgat
    36121 ggcgacaagc cagatcacga tgatgacact catcctgacg atgaacctcc gcagccacca
    36181 cgcggtggtc gaccggcatt acgcgttgtg aagtaataca aaacaggccc aggcggcctg
    36241 ttttgtcttt ttaatgactt aataaaatag cgtgattgtg gcaatcatat tcaccacgaa
    36301 accgactgcc atggggatcg ctgtccgctt caccaccgca aaaggcgaaa cgcctgcaat
    36361 tcctgacgta acgaccacca ccgcagtaat cggcgaaacc gcgcgcgcta gcgtcgtggc
    36421 aaaatgcatc ggcataatca ttacaaccgc tggtacatgt agtccggctg cgatattcgg
    36481 aataagactg gcaaatgaca taaacggcgc attgccagag cccatcacaa tggcacaaat
    36541 ggcaatgact agcgccataa taatcatcac gccaataccg cccagaccag aatgctccgc
    36601 acccctgata accgcatcaa cagtgccaat cgtcgttaag cctttcgcaa atatttcacc
    36661 cgcgaccacc agcgttacca cgttgacaaa ctgcgtaccc atgccgtcaa aaaacgcctg
    36721 cacatcgtcc attgtttcgc gcaagttatg cttgcggaag aactcaacaa acatcgtcac
    36781 cgtcaaactt agtaacatca ccaccaccag atgcagttct gactgcatca gcccgacgtg
    36841 ggcgaggaac agcgagccga gcatcaggat taacggcatc acaggtaaaa tggcgtaata
    36901 gagcggcggg acattatcga gagttttttg ctctgcctgt tcgtgattga tatttttatc
    36961 ttttttgtca aaagcgcgtt gcacgaaaaa gtgggagatc gccaccgaga taatgacgca
    37021 agaggcgacc ggaagctggt agtggaagaa gtatgtggca attttcattc ccgcgacctg
    37081 ggctgcaaaa atggagttcg tttccagaat cccccactca atggacatcg tggttgcgat
    37141 aaccgccacc gcagagaggc gacttactcc cagactcacc agcgtcggaa ataaggtgac
    37201 catcagcaac atacccagac cggaggcgct ggtaataaac tgcgccatga tttggccgat
    37261 gacgtaagtt gccgacagaa taatatacgg cgagcgaatg agttttaacg ggcggcttag
    37321 caagctcacc atcgcgcaac tggctcctat gcgctccatg tagcgggcat aaccgcccac
    37381 cgccataatc gacagcccca atccggcaat gcggttggac agcatgcgca ggatttcatt
    37441 ataaatgtcg acgatcaagt agccgctgct tttggcggga tcgagtacac caccgaaccc
    37501 taaccagact ccgcacatca tcaggaagac acctgcggca gccagcacca cctgaggttt
    37561 gtagtttttc aggatcaaat agcccatcgt aattaatacg atgacagata taattatgcc
    37621 gaacatt
//
LOCUS       DADLTY010000014        36868 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.014, whole genome shotgun sequence.
ACCESSION   DADLTY010000014 DADLTY010000000
VERSION     DADLTY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.014"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..868)
                     /gene="rpnA"
                     /locus_tag="JN334_000627"
     CDS             complement(<1..868)
                     /gene="rpnA"
                     /locus_tag="JN334_000627"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HAY7436881.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAA"
     gene            complement(1071..1307)
                     /gene="feoC"
                     /locus_tag="JN334_000628"
     CDS             complement(1071..1307)
                     /gene="feoC"
                     /locus_tag="JN334_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HAY7436882.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(1307..3628)
                     /gene="feoB"
                     /locus_tag="JN334_000629"
     CDS             complement(1307..3628)
                     /gene="feoB"
                     /locus_tag="JN334_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAY7436883.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAEDIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3645..3872)
                     /gene="feoA"
                     /locus_tag="JN334_000630"
     CDS             complement(3645..3872)
                     /gene="feoA"
                     /locus_tag="JN334_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAY7436884.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4310..6631)
                     /locus_tag="JN334_000631"
     CDS             complement(4310..6631)
                     /locus_tag="JN334_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAY7436885.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6728..7240)
                     /gene="greB"
                     /locus_tag="JN334_000632"
     CDS             complement(6728..7240)
                     /gene="greB"
                     /locus_tag="JN334_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HAY7436886.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANW
                     YVNAIEYVKP"
     gene            7432..8151
                     /gene="ompR"
                     /locus_tag="JN334_000633"
     CDS             7432..8151
                     /gene="ompR"
                     /locus_tag="JN334_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HAY7436887.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            8148..9500
                     /gene="envZ"
                     /locus_tag="JN334_000634"
     CDS             8148..9500
                     /gene="envZ"
                     /locus_tag="JN334_000634"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HAY7436888.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9576..11198)
                     /gene="pckA"
                     /locus_tag="JN334_000635"
     CDS             complement(9576..11198)
                     /gene="pckA"
                     /locus_tag="JN334_000635"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAY7436889.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11577..13328
                     /locus_tag="JN334_000636"
     CDS             11577..13328
                     /locus_tag="JN334_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HAY7436890.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            complement(13472..14341)
                     /gene="hslO"
                     /locus_tag="JN334_000637"
     CDS             complement(13472..14341)
                     /gene="hslO"
                     /locus_tag="JN334_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAY7436891.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(14366..14767)
                     /gene="hslR"
                     /locus_tag="JN334_000638"
     CDS             complement(14366..14767)
                     /gene="hslR"
                     /locus_tag="JN334_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HAY7436892.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14778..15446)
                     /gene="yrfG"
                     /locus_tag="JN334_000639"
     CDS             complement(14778..15446)
                     /gene="yrfG"
                     /locus_tag="JN334_000639"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HAY7436893.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15511..17646)
                     /gene="igaA"
                     /locus_tag="JN334_000640"
     CDS             complement(15511..17646)
                     /gene="igaA"
                     /locus_tag="JN334_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HAY7436894.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            17966..18526
                     /gene="nudE"
                     /locus_tag="JN334_000641"
     CDS             17966..18526
                     /gene="nudE"
                     /locus_tag="JN334_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAY7436895.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18691..21243)
                     /gene="mrcA"
                     /locus_tag="JN334_000642"
     CDS             complement(18691..21243)
                     /gene="mrcA"
                     /locus_tag="JN334_000642"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HAY7436896.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21363..22142
                     /gene="hofM"
                     /locus_tag="JN334_000643"
     CDS             21363..22142
                     /gene="hofM"
                     /locus_tag="JN334_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HAY7436897.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            22142..22681
                     /locus_tag="JN334_000644"
     CDS             22142..22681
                     /locus_tag="JN334_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HAY7436898.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22665..23105
                     /gene="hofO"
                     /locus_tag="JN334_000645"
     CDS             22665..23105
                     /gene="hofO"
                     /locus_tag="JN334_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HAY7436899.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            23095..23501
                     /gene="hofP"
                     /locus_tag="JN334_000646"
                     /pseudo
     CDS             23095..23501
                     /gene="hofP"
                     /locus_tag="JN334_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            23413..24651
                     /gene="hofQ"
                     /locus_tag="JN334_000647"
     CDS             23413..24651
                     /gene="hofQ"
                     /locus_tag="JN334_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HAY7436900.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            25052..25573
                     /gene="aroK"
                     /locus_tag="JN334_000648"
     CDS             25052..25573
                     /gene="aroK"
                     /locus_tag="JN334_000648"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAY7436901.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25630..26718
                     /gene="aroB"
                     /locus_tag="JN334_000649"
     CDS             25630..26718
                     /gene="aroB"
                     /locus_tag="JN334_000649"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAY7436902.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26810..28102
                     /gene="damX"
                     /locus_tag="JN334_000650"
     CDS             26810..28102
                     /gene="damX"
                     /locus_tag="JN334_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HAY7436903.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            28209..29045
                     /gene="dam"
                     /locus_tag="JN334_000651"
     CDS             28209..29045
                     /gene="dam"
                     /locus_tag="JN334_000651"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY7436904.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            29063..29740
                     /gene="rpe"
                     /locus_tag="JN334_000652"
     CDS             29063..29740
                     /gene="rpe"
                     /locus_tag="JN334_000652"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAY7436905.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29733..30491
                     /gene="gph"
                     /locus_tag="JN334_000653"
     CDS             29733..30491
                     /gene="gph"
                     /locus_tag="JN334_000653"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HAY7436906.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30484..31488
                     /gene="trpS"
                     /locus_tag="JN334_000654"
     CDS             30484..31488
                     /gene="trpS"
                     /locus_tag="JN334_000654"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAY7436907.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31758..32663
                     /locus_tag="JN334_000655"
     CDS             31758..32663
                     /locus_tag="JN334_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436908.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32680..33042
                     /locus_tag="JN334_000656"
     CDS             32680..33042
                     /locus_tag="JN334_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY7436909.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            33126..34289
                     /locus_tag="JN334_000657"
     CDS             33126..34289
                     /locus_tag="JN334_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HAY7436910.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            34290..35516
                     /locus_tag="JN334_000658"
     CDS             34290..35516
                     /locus_tag="JN334_000658"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY7436911.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            35513..36391
                     /locus_tag="JN334_000659"
     CDS             35513..36391
                     /locus_tag="JN334_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HAY7436912.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            36402..36755
                     /locus_tag="JN334_000660"
     CDS             36402..36755
                     /locus_tag="JN334_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HAY7436913.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36767..>36868
                     /locus_tag="JN334_000661"
     CDS             36767..>36868
                     /locus_tag="JN334_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436914.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHP"
BASE COUNT         8657 a         9314 c        10038 g         8859 t
ORIGIN      
        1 ccgcagccaa ctcttcttct gacagcccgg taaatcgcat gatgtctgca agagggactc
       61 cggattcaag cattattttg gctatatgca gggctttgga ttgttccccc tcctgccgca
      121 atctttccgc aatagtcatt aaactctcct tgtgtttcgg tgaacgttcg gcaacgccgt
      181 cgataaaatc gttaaaacgt acagcgtcgc cagtttgcag tatgtaatta aacagccctt
      241 tgatttgtct gtcattagcg tatccactac ttaataagca ggccatttgc tctaccagcc
      301 ccatcagatc gcgttgacga atatgttttt gaattaactc cagcagcgcc atacgtcggt
      361 gctgcatgat ttcatcatca ggcatgacgg tgacatcaat cagcggaaat gcggaggcat
      421 aaagctgcct tgccagtttg ggatcggcga aacaatccag ccaacacagc gaatagggat
      481 aggggctttc aataccgtgg taaaacaaca atggcaccac catcggcaac gttttgtatc
      541 cagcatcaag gtgattttgc attgcggcaa tagcgtaacg catcatgcga aatgcgatca
      601 gtttgtttga ggtgctttga tgttcaatca gacaatagat gtatcctggt ccttgttccg
      661 ttttcaccga ccacagcaca tcggaatagc tttcacgcag atcgtcatca ataaagctcc
      721 tcgactccag cttgagggtt ttcatatcac agagcgcgtg aatgggtgcc ggtaaatgaa
      781 acgcaagaaa atcgcgtgcc gtctccggtt ggcgtaaaaa gagtttgaac agcgcatcat
      841 gtggggtgga actctgcttt ttactcatgg cgttccttca cctgttgttt gaatgccgcg
      901 actctacgcg ccactccaca tctgtgcatt agcgagtttt catccctgcg agacgctttc
      961 agaggaactt acgttgcgct gcaagaattg catttttatg gaaagccgca cgcaaaaaag
     1021 cccggatgca aacgcatacc gggcttttca aaacaggcga tggagtatgg ttaacgcagc
     1081 gcccaccact cgcgcagaca ggctttgcct tccgggcagc ttttacaact gccagagagg
     1141 cagccgtcag gttcttcctg aatccgcacg gctttgccca tgctttccag ctgttgcagc
     1201 atggcgttaa tcattggctg cggagtgttc aatgtctggc ttatctgggt cgcttccata
     1261 cggccccgta acgccagcaa atcacgtacc tgaataagtg aagccattaa tggcaatcac
     1321 cggtggtgct ggctgcgcag caactgctta ccgacttgcg ggttgccagc agttcgatat
     1381 ccacccggct acgcgcgcgg cgcagcagac cgatagctac gatgttaaac aggattaccg
     1441 ccagaatgca caccaggctg taagttgggt gctgactgta gctggcgacc tgatagaaca
     1501 atgttgccag tgagtaagcg atattcagcc cccacaggat ggagaagccc atccagccac
     1561 ggcttgattc acgggcgatg gctcccatca ccgagataca tggtacatac agcaggacga
     1621 aaatcaggta gctgtatgcg gcagctgcgc taccgaattt ctgatccatc acgcccatcg
     1681 ccccggtacc catttcaccg ttgcctttgc tagcttcaat ggggttcatc agtacgctaa
     1741 ggctgaaggt gtctttcagg ctctgccagg tttcatctac cgcactgaac agctcttcac
     1801 cgaggttgaa ttccgccgga ttgaactctt cgtcctgaat atcttctgcg gtgtagaggg
     1861 tgttgagcgt acccaccacc acttcttttg ccattgcgcc cgtaaacagg ccaaccgttg
     1921 cctgccagtt atcttcatgt acgccaatcg gcttgaagac aggggttatc acccggctga
     1981 cggaagccag cgccgagtcg ttgatgttat cgacgatttt cccgctcaac gagaagctgt
     2041 tgaaagcgct caggaaaatg ctgacgatga taatcacttt accagcacgc agaacgaagc
     2101 ccttcagacg ctgccaggtc tggataatca ggcttttaac gtgtggtaca tggtagaccg
     2161 gcagctccat gacaaacggc gtcgcttcac cgcgcatgat ggtgtacttg agcatcaggc
     2221 cagtcagcac cgccatcacg ataccgagca tatacagcga gaaaaccgcc agggcaccgt
     2281 tctgcccgaa gaaggcagcc gcgaatactg cgaagatagc cagacgcgcg ccgcaggaca
     2341 taaacggtgc catcatgatg gtcatcagac gttcacgcgg tgcatcaagc gtacgtgcgc
     2401 ccattaccga cggtacgtta caaccgaaac cgacgatcag cggcacaaag gatttccccg
     2461 gcaagcccag cgcctgcatc aggcggtcca tcacaaacgc tgcacgcgcc atatagccgg
     2521 agtcctcaag gaaggagagg aacaggtaca tcatgccgat ctgcggcacc agtggcagca
     2581 cggtgttaat accgccaccc aggccctggg cgaggaagat agtcagccag tccgggaagt
     2641 ggagcgtgta gccaatccat tgaataccat gcacaaatag cgccacggag ccgacgttaa
     2701 acagcggctg taacgccccg ccgatgttga tagccagcag gaacatcagg tacatcacaa
     2761 agaggaaaat cggcagaccg aggaaacggt tgagcacgat tttatctacc gcagtggtga
     2821 aacggctggg ttctgccgtc agggtgttgc ttaccacatc acagatggca gcaatgcact
     2881 ggtaacgcgc atcggcaatg tgcagcgccg gatcatccat ttcattacgc agacgggcga
     2941 gggtggcatc cagatgctgc gacgcttcac cggcgtaggc gcggctgtag atatcgcctt
     3001 ccagcatttg caggcccagc cagcgacgtt gtttcagcgg gatgtcggaa ggcatcactt
     3061 ttgccagtga atctgcttcg ttgagtagcg gctgtgcgta atgcaccagt tccacattct
     3121 cgttagcttt atagcgatca atcgccagct tgagcgcttc aataccgcga ccacgggttg
     3181 aaaccagcgg gatcaccggg cagccaagac gtgccgatag agcatcaatt tcaatacgaa
     3241 tattttgctt ctcggcaatg tcgagcatgt tcagcgccac gatgcaggga atgccgagtt
     3301 ccagcagttg cagcgtcagg tacaggttac gctcaaggtt agacgcatcc accacgttaa
     3361 tcagcaggtc ggcgtcgcca ctcaaaatgt agtgacaggc gatttgctca tcgagcgagg
     3421 tctgcgatga gatggtggtc agagaatagg tgccgggcag gtccaccagc gtgacctgat
     3481 gatcggtggt ggagaattgc ccttctttac gttcgacggt aacgccagcc cagttaccta
     3541 cacgctgacg tgcgccagtg agctggttaa ataaggttgt cttgccagaa tttggattac
     3601 caattaagcc aatggttaat tttttcattg ttgttatcac cgtattaaca ggaaaccgct
     3661 tccacttcta ataaggccag atcttttttg cgtaatacca ggctcacacg acgggtttcg
     3721 atatgaatgg ggtcgccgag tggagcgacg cgcaccacat caaaagagga gccaggtaac
     3781 atgcctagag aaagcagttt ttggcgatat gccgggctga tttcacggga aaagccagtg
     3841 attttccacg cagtatctgg agtgtattgc ataggtgcct acttgtttct cattaactgg
     3901 ataactacct catggggcgt ttgaccgcga catgagacca gatgatttgc caacaaagga
     3961 gagattaaat aatcaacaac acaatgataa tgagaatggt ttctatcagc aatacttaaa
     4021 atgtgtgcga atgttggttt gaataataat ctgactcaat gttgatgtgg ctcaaggtta
     4081 tatcgcagcg aaaatataat tcgaatagtg agttacccgg actggctaat gtttaattaa
     4141 ggtttcagtt gttaatgaag tgataaatat taacggagta tattgtgagt ttggctgtgg
     4201 aataaaagtg ccggagatat tctccggcac ttttatgtca ggcaaagcgt tcgttgccag
     4261 atgcggcgtg aacgccttat cgcgcaggtt taggggccag tccgggcgtt taacgttttt
     4321 tacccatcgc tgccgccagc gcatccatca tcgcgctatt accggcaggc tgcgcttcac
     4381 gaccgcgtgg tttggctgcc gggcggttgt tttgcgggcg ttcattaccg ccgccgcgac
     4441 gagagttggt ttcgccaggc tgctcgtcca gacgcatagt cagggcgata cgtttacgct
     4501 gaagatccac ttccagcact ttcaccttca caatgtcgcc cgctttcacc acggtatgcg
     4561 gatcttccac aaacttgttc gacaatgaag agatgtgaac caggccgtcc tgatgcacgc
     4621 caatatcgac aaacgcgcca aagttggtga cgttggtcac tgcgccttcg aggatcatac
     4681 ccggttgcag gtcgttcatt gtctcgacgc catcggcaaa ctgagcggtt ttaaattccg
     4741 gacgcggatc gcgacccggt ttttccagct ctttgatgat gtcagttact gtcggcacac
     4801 cgaatttttc atcagtaaag tcagacgctt tcaggttacg cagttcgctg ctgttaccca
     4861 tcagatcttt cagtgcctgc tgtgttgctg ccagaatgcg ttccaccacc ggataggctt
     4921 ccggatgaac ggtagaggcg tccagcgggt tatcaccgtg gttaatgcgc aggaagcccg
     4981 cgcactgctc gaaggctttc ggccccagac ggctgacttt cagcagttgc tgacggttct
     5041 ggaactggcc gttctcatcg cgccaggcca cgatgttttg cgccatcatg cgcgtcaggc
     5101 ccgccacgcg ggttaatagc ggaacagaag cggtgttgag atcgacgcca acggcgttta
     5161 cgcagtcttc tactactgca tccagtttgc gggccagttg cgtctggctg acgtcatgct
     5221 gatactgacc tacgccgata gatttcggat cgattttcac cagctccgcc agcggatcct
     5281 gcaaacggcg ggcgatagat accgcaccac gcagcgaaac gtcgagatcc gggaactcct
     5341 gcgctgccag ctcggaagct gagtaaaccg acgcgccagc ttcgctgacg atcactttct
     5401 gtgcggtcac tttcgggaac tgcttctgca cgtcgagata gaaacgttca gtttcgcggg
     5461 aagctgtacc gttgccgatc gccaccagtt caacgttatg tttttcacac aaggcagcaa
     5521 cggtcatcgc tgcttttgcg gcttgtccgg tgtgcgggta aatggtatcg gtcgccacca
     5581 gtttgccagt ggcatcgacc accgccactt ttaccccggt acgcagaccc ggatcgaggc
     5641 ccatcgttgc acgcagtccg gcaggggccg ccatcagcag atcgtgcagg ttacgggcaa
     5701 agacgttgat tgcttcatct tccgcgcgtt cgcgcacggt acccatcagt tcggtttcca
     5761 gatgcatcag caccttgatg cgccaggtcc agctcactac gcttttgcgc cagctatccg
     5821 ccggggcatt gttcaggcgc aggccaaggt gatccatgat gatttgctcg caatagctct
     5881 ctttgggcgg ctcatcgaac tgcggatcgg cattcagcga aagctggagt acgccttcgt
     5941 tacgcccacg gaacatcgcc agcgcgcggt gagaaggcac cgtggacaac ggttcgtgat
     6001 gatcgaaata gtcgcggaat ttcgcccctt cctcttcttt accgctcacc accgtagaaa
     6061 ccaaatgcgc gttcttccac agataatcac gcactttcgc cagcagcgcg gcatcttcgg
     6121 caaaccgttc catcaggata tagcgcgcgc catccagcgc ggctttggta tctgccacgc
     6181 ctttatcggc atcaacatat tgtgcagcgg cgacttctgg cgtgtgtgac ggatcgctcc
     6241 acagcaggtc agccaacggc tcaagccctg cttcaatggc gatttgcccg cgggtgcggc
     6301 gtttaggttt gtagggcagg tagaggtctt cgagttcggt tttgcttagg gtggcgttga
     6361 tggccttcgc cagatcatcg gtgagtttgc cttgctcgga aatggatttg aggatcgcct
     6421 gacgtctctc ttccagctcg cgcagatagc tcagacgcgt ttccagatta cgcagctgcg
     6481 tgtcatccag accgccggtg atttccttac gataacgtgc gataaacggc acggtattcc
     6541 cttcgtcaag caggcgaacg gcagcgtcaa cctgttccgg gcgcgcctga atttcacccg
     6601 caataatgcg gcagaacgaa tcattcatca tggtttgagt tcatcttttc ggatcaaaaa
     6661 tcaggggata gttatacgga ctggctggca aaaatgccag ccatcggcag gaggttaaga
     6721 ctcttcctta cggtttcacg tactcgatag cattaacata ccagttcgct tccccggcag
     6781 gggtattcac caccgccaga tcgccgactt cttttttcag caatgcgcgg gccatcgggg
     6841 aatcgataga gatgtaatct ttacggccaa aaatttcatc gtagccgaca atacggaaac
     6901 ggtgagtcac gccatcgtcg ttttcaatct ccacccacgc gccaaaaaag actttgcctt
     6961 cctgctgagg ggagtaatcg acgattttga gattttccag gcatttagtg agatagcgca
     7021 cgcgacggtc gatttcacgc agacgctttt tattatactg atagtcagca ttttcgctgc
     7081 ggtcgcccag acttgcggcc caggtcacct tttttgtgac ctccgggcgt tcttcacgcc
     7141 agagataatt aagctcttgt ttgagttttt catacccttc ccgggtaacc aggggcgttt
     7201 tcatctcgtt gattcccttt gtctgtttga taatgcgcac attgggtata acgtgatcat
     7261 atcaacagaa tcaataatgt ttcgccgaat aaattgtata cttaagctgc tgtttaatat
     7321 gctttgtaac aatttaggct gaaattcata ccagatttag ctggtgacga acgtgagctt
     7381 ttttaagaat acacgcttac aaattgttgc gaacctttgg gagtacaaac aatgcaagag
     7441 aactacaaga ttctggtggt cgatgacgac atgcgcctgc gtgcgctgct ggaacgttat
     7501 ctcaccgaac aaggcttcca ggttcgaagc gtcgctaatg cagaacagat ggatcgcctg
     7561 ctgactcgtg aatctttcca tcttatggta ctggatttaa tgttacctgg tgaagatggc
     7621 ttgtcgattt gccgacgtct tcgtagtcag agcaacccga tgccgatcat tatggtgacg
     7681 gcgaaagggg aagaagtgga ccgtatcgta ggcctggaga ttggcgctga cgactacatt
     7741 ccaaaaccgt ttaacccgcg tgaactgctg gcccgtattc gtgcggtgct gcgtcgtcag
     7801 gcgaacgaac tgccaggcgc accgtcacag gaagaggcgg taattgcttt cggtaagttc
     7861 aaacttaacc tcggtacgcg cgaaatgttc cgcgaagatg agccgatgcc gctcaccagc
     7921 ggtgagtttg cggtactgaa ggcactggtc agccatccgc gtgagccgct ctcccgcgat
     7981 aagctgatga accttgcccg tggtcgtgaa tattccgcaa tggaacgctc catcgacgtg
     8041 cagatttcgc gtctgcgccg catggtagaa gaagatccag cgcatccgcg ttacattcag
     8101 accgtttggg gcctgggcta cgtctttgta ccggacggct ctaaagcatg aggcgattgc
     8161 gcttctcgcc acgaagttca tttgcccgta cgttattgct catcgtcacc ttgctgttcg
     8221 ccagcctggt gacgacttat ctggtggtgc tgaacttcgc gattttgccg agcctccagc
     8281 agtttaataa agtcctcgcg tacgaagtgc gtatgttgat gaccgacaaa ctgcaactgg
     8341 aggacggcac gcagttggtt gtgcctcccg ctttccgtcg ggagatctac cgtgagctgg
     8401 ggatctctct ctactccaac gaggctgccg aagaggcagg tctgcgttgg gcgcaacact
     8461 atgaattctt aagccatcag atggcacagc aactgggcgg cccgacggaa gtgcgcgttg
     8521 aggtcaacaa aagttcgcct gtcgtctggc tgaaaacctg gctgtcgccc aatatctggg
     8581 tacgcgtgcc gctgaccgaa attcatcagg gcgatttctc tccgctgttc cgctatacgc
     8641 tggcgattat gctattggcg ataggcgggg cgtggctgtt tattcgtatc cagaaccgac
     8701 cgttggtcga tctcgaacac gcagccttgc aggttggtaa agggattatt ccgccgccgc
     8761 tgcgtgaata tggcgcttcg gaggtgcgtt ccgttacccg tgcctttaac catatggcgg
     8821 ctggtgttaa gcaactggcg gatgaccgca cgctgctgat ggcgggggta agtcacgact
     8881 tgcgcacgcc gctgacgcgt attcgcctgg cgactgagat gatgagcgag caggatggct
     8941 atctggcaga atcgatcaat aaagatatcg aagagtgcaa cgccatcatt gagcagttta
     9001 tcgactacct gcgcaccggg caggagatgc cgatggaaat ggcggatctt aatgcagtgc
     9061 tcggtgaggt gattgctgcc gaaagtggct atgagcggga aattgaaacc gcgctttacc
     9121 ccggcagcat tgaagtgaaa atgcacccgc tgtcgatcaa acgcgcggtg gcgaatatgg
     9181 tggtcaacgc cgcccgttat ggcaatggct ggatcaaagt cagcagcgga acggagccga
     9241 atcgcgcctg gttccaggtg gaagatgacg gtccgggaat tgcgccggaa caacgtaagc
     9301 acctgttcca gccgtttgtc cgcggcgaca gtgcgcgcac cattagcggc acgggattag
     9361 ggctggcaat tgtgcagcgt atcgtggata accataacgg gatgctggag cttggcacca
     9421 gcgagcgggg cgggctttcc attcgcgcct ggctgccagt gccggtaacg cgggcgcagg
     9481 gcacgacaaa agaagggtaa ataaacggga ggcgaaggtg cctcccgttt tgctttctat
     9541 aagatactgg atagatattc tccagcttca aatcattaca gtttcggacc agccgctacc
     9601 agcgcggcac ccgcaggggt gtcggtgtat ttatcgaagt tgtcgataaa cagtttcgcc
     9661 agggtttcgg ctttttcctg ccactgttcc ggagaagcgt aggtgttacg cggatcgaga
     9721 atcttcgtgt ctacgcccgg cagttcggtt gggatcgcca ggttaaacat cggcagagtg
     9781 aaggtttctg cattatccag cgaaccgttg aggatggcgt cgataatggc gcgggtatct
     9841 ttaatcgaga tacgtttgcc agtgccgttc cagccagtgt taaccagata agcctgcgcg
     9901 cccgccgcct gcatacgttt caccagcact tctgcgtact gagtcgggtg cagcgacagg
     9961 aacgccgcgc cgaagcaagc ggagaaggtt ggcgtcggtt cggtgatgcc acgctcagta
    10021 ccggccagtt tggcggtgaa gccagagagg aagtgatact gggtttgatc ggcagtcagg
    10081 cgagaaaccg gcggcaacac gccgaaagca tcagcagtca ggaagataac cttagtcgcg
    10141 tggcccgctt tggaaaccgg cttaacgatg ttatcgatgt gatagattgg ataagaaacg
    10201 cgggtgttct cggtttttga accatcatca aagtcgatag tgccatcttc acgcacggtg
    10261 acgttttcca gcagcgcatc acgacggata gcgttgtaga tttcaggttc cgcttctttc
    10321 gacagcttga tagtttttgc gtagcagccg ccttcgaaat taaacacgcc gtcatcgtcc
    10381 cagccgtgtt cgtcatcgcc aatcaggcgg cgtttcgggt cggtggaaag ggtggtttta
    10441 ccggtgccgg aaaggccgaa gaacaccgca acatcgcctt tctcgccaac gttggcggag
    10501 cagtgcatag aagcgatacc tttcagcggc agcaggtagt tcatcatcga gaacatccct
    10561 ttcttcattt cgccgccgta ccaggtgccg ccaatcagct gcatgcgctc ggtcaggtta
    10621 aacgccacga agttttcgga gttcagaccc tgttctttcc actgcgggtt agtgcacttc
    10681 gcgccgttca taacgataaa gtctggtttg aaacctgcca gctcttcatc gctcgggcga
    10741 ataaacatgt ttttgacaaa atgcgcctgc caggccactt cggtgatgaa acggacggaa
    10801 agacgagtat ccgggttcgc accacagaaa gcgtcgacaa cgaacagacg tttgccggaa
    10861 agctgcctgg tcaccaggcc tttcagatgc tgccaggttt ccggagagag aggtttgttg
    10921 tcgttcttac ctttgccttt gtctgcccac cagaaagtat cgcgagtggt atcgtcacgg
    10981 acgatatact tatcttttgg tgaacgaccg gtgaagatcc cggtatcgac ggcaacggca
    11041 cccagattag ttaacacccc gcgctcataa cctgtcaggc tcggatcgag ctcttcctga
    11101 tacagcaggt cgtagcttgg gttgtaaacg atatcatgta cgtcactgat accataagcc
    11161 tcgagttctt gcgggggcaa accattgtta acgcgcattt cactgctcct tagccaatat
    11221 gtattgcctg aatagtaaag tctttttggg ggtgttaacc gcgacaaggc tcatagattt
    11281 acgtatctgg agaaattcat atttgacaaa aaactccgtg attcctgtca cgaaacggtt
    11341 gctattatcg cagaaatagc attttaggtg tggaaaatgt tctcaaaagg ttaaatcttg
    11401 gtttatatgg cgggaatatt gtgagtggaa tcgcattcct gaatgaaatt gatgcaaatt
    11461 caaccggtaa agtatcgatt caccacgcta actttttttg attattttct gaaaaaagca
    11521 aaatactact gcccggatgc gataattaac ccaccttttg attctggata accaacatgg
    11581 ataacgttga actttcaccg gcaacacgct gggggatgat tgctaccgga ttacttcagg
    11641 ggatgattgc taccggatta cttcaggggc tggtttgcta cctgcttatc gcctggcttg
    11701 ccggaaaaaa tcacagttgg attgtttatg gcgtgcctgc gaccgtcgcg ttttcatccg
    11761 ttttactctt ctccgtgatc tcttttaaac agaagcgcct ttggggatgg ctggcgctgg
    11821 tgtttattgc cacgacaggg atgagcggtt ggctgaagtg gcaaactgat ggcatgaacc
    11881 cctggagagc tgaaaaggcg atttgggatt ttggctgcta tctgctgttg atggcaatgt
    11941 tgttgctacc gtggatacaa caaagccttc gtatccgcaa tggcagtagc cgctatagct
    12001 acttttacca gtcagtatgg cataacgtac ttatattact ggtgattttt ctctccaatg
    12061 gcctgacgtg gctggtgctt ttactgtggg gtgagttgtt taaacttgtt ggcatcacgt
    12121 ttttcaatac gctctttttt gcaaccgact ggtttatgta tcttacattt ggtctggtta
    12181 ccgcactggc ggtgatcctc gcgcgtacac agtcacgttt aatcgactct attcaaaagt
    12241 tgttcacgct aatcgccacg gggttgctgc cgttagtatc attgctaacc ctgatgttta
    12301 tcatcaccct gccgtttacg ggcctgagcg cgatttctcg ccacatctcc gccgccgggt
    12361 tgctgttaac gctggccttt ttgcaattga tcttaatggc tattgtccgc gatccgcaaa
    12421 aagcgtcact tccctggacg gggccgttgc gttgcctgat taaaaccgct ttgctggttg
    12481 ctccgctgta tgtgttcgtc gccacctggg ggttatggct gcgggtcgct cagtacggct
    12541 ggactgtcga ccgcttgcag ggcgcgctgg cggagctggt gttactggtg tggtcgctgg
    12601 ggtattttgt cagtatcgtc tggcgtaatg ggcaaaatcc ccttgttctt cagggcaaag
    12661 tgaaccttgc ggtttcgtta ttggtgttgg tgatactggt gcttcttaat tcgccggtgc
    12721 tggacagtat gcgtattagc gtgaacagtc atatggcgcg ttatcagagc ggcaaaaaca
    12781 cgccagacca ggtaactatc tacatgctcg aacagagcgg tcgctatgga cgtgcggcgc
    12841 ttgagtcgct gaaaagcgat gccgggttta tgaaagaccc gaaacgcgcg cgggatctgc
    12901 tgatggcgtt agatggagag caacatcttc aggaacaggt atcggaaaaa gtattcgccg
    12961 aaaatgtgtt aattgcccct ggttctgtta aaccggatgc gacattctgg tcggccttaa
    13021 tccaggatcg ctataacgtg atgacctgta ttgaaaaaga cgcctgcgta ctggtcgagc
    13081 aagatctgaa tagtgatggt caggcggagc ggatcctgtt tgcttttaat gatgacagag
    13141 tcattgtcta tggctttgac tcagacagaa aagaatggga cgcgcttgat atgagtttac
    13201 ttccgaacga aataacgaaa gaaaaattac tcacagctgc gaaggatgga aaactgggga
    13261 cgaggccgaa agcgtggcgc gatctgacgg tagatggtga aacgctggaa ataaacttaa
    13321 gtaagtgaca acgcttttat cggatacgac gctgtatctg accaaaataa tttgcgattt
    13381 atttcatagg cgctggtaag cgcagcacat caggccatta agtcgtaaaa tccggcagag
    13441 ccctctctgc cggacatact cattaatgaa cttacggatc tgccggagac gcgttgttgc
    13501 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13561 tgtcaatttc gccatcttcc gccaggatgc tatcaacttc ttcatcaggc agcgttttca
    13621 gcgcatcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13681 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13741 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgtt
    13801 gggtattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13861 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13921 ctaggcaggc cgccagggta tcaccttcca gaccaactac gccctgatag cgttcgcctt
    13981 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    14041 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    14101 taataaccgc cagattcatc ggaccgtcgc cctgcagctg tacggtgata tcaccatcaa
    14161 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    14221 cgggctgcgg ataatcgtgg ttctcaagga tctgttgcag ggtttccgaa acggttacca
    14281 gttcgccgcg aacggcaaaa ttttcaaaca gatagcgatg taattggtca tgttgcggca
    14341 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14401 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14461 tacgtgccat cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14521 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14581 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14641 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14701 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggtttct
    14761 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14821 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14881 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14941 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    15001 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagacccgta
    15061 tgctcaagtt ttaccgccag gttgtgcgga tgagcattgg tgagcaaaat tcgctgctta
    15121 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcgcgcggt
    15181 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    15241 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    15301 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15361 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15421 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15481 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15541 cagttgcggg ttcaggcagc tttcataata ggcctgaatc tccatcatgc gagtgcgatg
    15601 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15661 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga tcggatgaag
    15721 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cttcggcgtt
    15781 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15841 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15901 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15961 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attcagcact
    16021 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    16081 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    16141 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    16201 taccatgccg gatttttgaa tcgctgaacg taatgaggca ctgacgcgag attcatcttc
    16261 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    16321 ttcagattct ggtaatggta atgagcgggc gtcattccag atgatctgcg agcagtcaaa
    16381 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16441 tcccgtaccg ctaatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16501 tacgctggtg gcttcaatgg tctgcgcgcc tttcatccat gagagagtga atttcagcgg
    16561 catatccagc gggatccaga ataacagcat aaacagcact agcagcgagc ccgcagcgat
    16621 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16681 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16741 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16801 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16861 accgcgcaga caatgaattt ctcgcaggga ggattttgcc gggggcgcga atagccccca
    16921 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16981 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    17041 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    17101 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    17161 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccaa
    17221 cggcagcgaa tcggtatgaa tcagttcaac ggtattctca tcgttgatgt actgttccca
    17281 gaaggggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    17341 ggtagagatg ccgtaacgtg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17401 attcagcgcc agagagatcg gtgccgcgct agcttccgtt gggccaggca cctgtaatac
    17461 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17521 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17581 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17641 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cggacacaat
    17701 acatgattct gcggttggct atcggcaagc gtggagctta acttgagcat cattaagcac
    17761 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg gaatatcagc aaattcctgg
    17821 agttatttca acctattgac ttttaatttg atttgaggac gattatttat taagttacat
    17881 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17941 ctgatttcac agcatagatc ccacgatgaa caaatcatta caaaaaccca ccattctgaa
    18001 tgttgaaact gtagcccgtt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    18061 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    18121 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    18181 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    18241 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    18301 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18361 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18421 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18481 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18541 cctgatgcac aacgcttatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18601 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18661 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18721 gataatggtc gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18781 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18841 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18901 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18961 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    19021 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    19081 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    19141 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    19201 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    19261 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    19321 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19381 gtaaatcact ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19441 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccactagga agccgccgtt
    19501 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19561 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19621 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19681 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19741 gtcagaaccg gcacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19801 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19861 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19921 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19981 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    20041 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    20101 ccagcgaacg ccttccatac tcaatgcgac ggtcgacccg tccgccagca tcgccgtcgc
    20161 tccctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    20221 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    20281 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    20341 ctgctgcgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20401 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20461 cgcagagaaa gcaatctccg gcgcgtgata attagcgtta atcgcctcag tgcgtgtctg
    20521 atcgaactgt tgctgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20581 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcaa
    20641 cccggctatc accgccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20701 ttgtgccgca gcaccgacac cataggcgcg gtaaccaagg taaatcttgt tcagataaag
    20761 ctcgaggatt tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20821 aatcttacgc atcatcgtgc gttctggact gaggaagaaa tttctcgcca gctgctgggt
    20881 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20941 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    21001 ggctttcacc atctccggtg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    21061 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    21121 atcttttaat gtcaccacat ccggcagttg tggctcgatg tagcggtata ggccataaat
    21181 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    21241 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgc gcggtagtat
    21301 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21361 caatgacatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcggcagcgg
    21421 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21481 cgcacgacat tatcaaagat gggcggattg ttgatgcgca gcagctggct aaaacgttgt
    21541 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21601 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct tggtgagcgg gagcaaacgg
    21661 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgcttcg
    21721 attacagcga agactcactc agccccgctt ataacgtgac tgccgcgcaa agcaaagagc
    21781 tggcaacgct gcttacgctg gcagaaaggt tgcgtgttca tgttagtgcg atcaccccgg
    21841 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21901 ggcgtgataa cgaacagtgg ctgtgggcga cgcgctatag ctgggggcgc aaactggcgg
    21961 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gacagcgtcg
    22021 cgatatgtgg cgaaggcgga tttgacccct gggaggccgt ttctgttcgt cagccgccgc
    22081 taccgccgcc tggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    22141 gatgaatccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttctgcg
    22201 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    22261 gcgtctgaca ggcagcgccg aagcgcgcat agacgcagtt ttgcttcagg cggaacaaca
    22321 actcgcccgc agcttacaga taacgaagcc acgtttgctg gagcaacagc agttacgcga
    22381 acagcgttcg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22441 agcactggcg gcgcttttac ccgagcacgc ctggctgaca acgataagct ggcagcaggg
    22501 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22561 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22621 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22681 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22741 tgttagttac gctcattttt ctatcatcga cacaccatga agagcgcgac gcattaattc
    22801 gactacgggc aagtcatcac cagcagtggg ccgcactgta tcgcctggta gacaccactc
    22861 ccttcagcga ggaaaaaacg ctgccctttt cgccactgga ttttcagtta tccggcgcgc
    22921 aactggtttc ctggcatcca tccgcgcagg gaggtgagtt ggcgttgaaa acgctgtggg
    22981 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    23041 cgttaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagatg ccgcatgagg
    23101 gttaaacgct ggttgttggc aggtattgca ttgtgccttt taaccggtat gcgtgaccct
    23161 tttaaatcgc cggaagatct atatgccgga ttagcgaact tagccagtgg cgctatcagg
    23221 ggatggtagg gcgaggcgag cgcatcatcg gtgtaataaa agacgggcaa aagaaatggc
    23281 gacgggtgca gcaaaacgat gtgctggaaa acggctggac aattttacag ctgacgccag
    23341 acgtactaac gctgggtacc gggacaaact gcgaaccgcc acaatggttg tggcaacggc
    23401 aaggagatac aaatgaagca atggatagcc gcactactgt tgatgctgat acccggcgta
    23461 caggcggcaa agccgcaaaa agtgacgctg atggtggatg acgttccggt agctcaggtg
    23521 ttgcaggcgc tggctgaaca ggagaagttg aacctggtcg tgtcgccaga cgtcagcggt
    23581 acggtgtcgt tacatctaac agatgttccc tggaagcagg cactacaaac tgtagtgaaa
    23641 agcgccggac tgataacgcg gcaggaaggc aacattctct cagtgcattc cattgcctgg
    23701 cagaatgaca atatcgcccg ccaggaggcg gagcaggcgc gggcgcaggc aaatctgccg
    23761 ctggaaaatc gcagtataac cctgcaatac gccgacgcgg gagaactggc gaaagcgggg
    23821 gagaagctac tgagtgccaa agggagtatg accgtcgata aacgcaccaa tcgccttttg
    23881 ctacgagata acaaaacggc gttaagcgcg cttgaacagt gggtagcgca aatggatctg
    23941 ccggtcgggc aggttgagct gtcggcgcat attgtcacca ttaatgaaaa aagtttgcgt
    24001 gagttaggcg tgaaatggac gctggccgat gcgcaacacg ctggtggcgt tgggcaagtc
    24061 accacgcttg gtagcgacct ctccgtagcg acggcgacaa cgcatgtcgg ttttaacatt
    24121 gggcgcatca acggacgctt gctggatctt gagctttccg cgctcgaaca aaaacagcag
    24181 ctggatatta tcgccagtcc gcgtctgctg gcctcacatc ttcagcctgc cagcattaaa
    24241 caggagagcg aaattccata tcaggtttcc agcggggaaa gtggcgcgac gtcggtggaa
    24301 tttaaagagg ccgtcctggg gatggaggtc acgcccacgg tgttacaaaa aggtcgcatc
    24361 cggctgaaat tacacatcag ccagaacgtt ccggggcagg tgctacagca ggccgatggc
    24421 gaagtgctgg cgattgataa gcaggagatc gaaacgcagg tcgaggtaaa aagcggagaa
    24481 acgttggcgc tgggcggcat ttttacccgt aaaaataaat cgggtcagga tagcgtaccg
    24541 ttgcttggcg acattccctg gttcgggcaa ttatttcgtc atgacggaaa agaagatgaa
    24601 cgacgcgagt tagtggtgtt tatcacgcca cgactggttt ccagtgagta aacagccgta
    24661 aaagcggtaa tgtttttatg ctgaacgtgt ttcatctatt tgacgcgcgc aggtatttag
    24721 catacaagga gtaccgattt gagagttggt gctcttcgct gcctgcgttc catgatgatg
    24781 atttatcatt caggcggcat tttgctgtct tttttacgct aatcttaccc ggtgatttat
    24841 cgccagagcg gtggtagcaa ggcagcgcgc ttgcagcgac cagatatgca gagggatggg
    24901 tgatttattc agttgccaaa cccgctggag tattgagata attttcagtc tgactctcgc
    24961 aatatcttat gaggtttcag ttcatgtcct gcggcgctct ctgagcgaag cgggtttatc
    25021 attaacgaat agtcttagta gtaccgaaaa aatggcagag aaacgcaata tctttctggt
    25081 tgggcctatg ggtgccggaa aaagcactat tgggcgccag ttagctcaac aactcaatat
    25141 ggaattttac gattccgatc aagagattga gaaacgaacc ggagctgatg tgggctgggt
    25201 tttcgattta gaaggcgaag aaggcttccg cgatcgcgaa gaaaaggtca tcaatgagtt
    25261 gaccgagaaa cagggtattg tgctggctac tggcggcggc tctgtgaaat cccgtgaaac
    25321 gcgtaaccgt ctttccgctc gtggcgttgt cgtttatctt gaaacgacca tcgaaaagca
    25381 acttgcacgc acgcagcgtg ataaaaaacg cccgttgctg cacgttgaaa caccgccgcg
    25441 tgaagttctg gaagcgttgg ccaatgaacg caatccgctg tatgaagaga ttgccgacgt
    25501 gaccattcgt actgatgatc aaagcgctaa agtggttgca aaccagatta ttcacatgct
    25561 ggaaagcaac taattctggc tttatataca ctcgtctgcg ggtacagtaa ttaaggtgga
    25621 tgtcgcgtta tggagaggat tgtcgttact ctcggggaac gtagttaccc aattaccatc
    25681 gcatctggtt tgtttaatga accagcttca ttcttaccgc tgaaatcggg cgagcaggtc
    25741 atgttggtca ccagcgaaac cctggctcct ctgtatctcg ataaggtccg cggcgtactt
    25801 gaacaggcgg gtgttaacgt cgatagcgtt atcctccctg acggcgagca gtataaaagc
    25861 ctagctgtac tcgataccgt ctttacggcg ttgttacaaa agccgcatgg tcgcgatact
    25921 acgctggtgg cgcttggcgg cggcgtagtg ggcgatctga ccggcttcgc ggcggcgagt
    25981 tatcagcgcg gtgttcgttt cattcaagtc ccgacgacgt tactgtcgca ggtcgattcc
    26041 tccgttggcg gcaaaactgc ggtcaaccat cccctcggta aaaacatgat tggcgcgttc
    26101 taccagcctg cttcagtggt ggtggatctc gactgtctga aaacgcttcc cccgcgtgag
    26161 ttagcgtcgg ggctggcaga agtcatcaaa tacggcatta ttcttgacgg tgcgtttttt
    26221 aactggctgg aagagaatct ggatgcgttg ttgcgtctgg acggtccggc aatggcgtac
    26281 tgtattcgcc gttgttgtga actgaaggca gaagttgtcg ccgccgacga gcgcgaaacc
    26341 gggttacgtg ctttactgaa tctgggacac acctttggtc atgccattga agctgaaatg
    26401 gggtatggca attggttaca tggtgaagcg gtcgctgcgg gtatggtgat ggcggcgcgg
    26461 acgtcggaac gtctcgggca gtttagttct gccgaaacgc agcgtattat aaccctgctc
    26521 acgcgggctg ggttaccggt caatgggccg cgcgaaatgt ccgcgcaggc gtatttaccg
    26581 catatgctgc gtgacaagaa agtccttgcg ggagagatgc gcttaattct tccgttggca
    26641 attggtaaga gtgaagttcg cagcggcgtt tcgcacgagc ttgttcttaa cgccattgcc
    26701 gattgtcaat cagcgtaaca acaagaaagg tcaggccgct tatcaagcgg tctattagct
    26761 tcaggttaat tgcaacgtgg taagcattaa ccttttagtg gggtgttaaa tggatgaatt
    26821 caaaccagaa gacgagctga aacccgatcc cagcgatcgt cgtactggtc gttctcgtca
    26881 atcttctgaa cgttctgagc gtactgaacg tggcgaaccg cagatcaatt ttgatgatat
    26941 tgaacttgat gacactgacg atcgccgtcc gactcgtgcg caaaaagagc gcaatgagga
    27001 accggaaatc gaagaagaaa ttgacgaatc cgaagatgaa accgtggatg aagagcgcgt
    27061 agagcgtcgt ccgcgtaagc gcaaaaaagc agccagtaaa cccgcttctc gtcagtatat
    27121 gatgatgggc gtcggcattc tggttctact gctgttgatc atcggtatcg gttctgcgct
    27181 aaaagccccc tcgaccactt ccagcgatca aaccgcgtct ggcgagaaga gtattgatct
    27241 tgctggcaat gcgaccgatc aggcgaatgg cgtgcagcca gcgccgggaa ccacgtctgc
    27301 ggaaaatact cagcaggatg tttctctgcc accgatctct tctacgccga ctcaagggca
    27361 aaccccggtg gcaacggatg gtcaacaacg tgttgaagtg cagggtgacc tgaacaatgc
    27421 gctgacccag ccacaaaatc agcaacagct gaacaatgtg gcggtcaatt ccacgttgcc
    27481 gactgaaccc gcaacggttg cgcctgttcg caatggcaat gcatcgcgtg acacggcgaa
    27541 aacgcaaacc gctgaacgtc cgtccactac gcgcccagtt cgtcagcagg cggtgattga
    27601 accgaaaaaa ccgcaagcaa ccgtgaaaac ggagccgaag ccggtagcac agacgccgaa
    27661 gcgtactgaa cctgaaccag ctgcccctgt ggcgagcacg aaggcaccgg ctgcgacttc
    27721 tacgccagca ccaaaagaga cggcgactac ggctccagta cagacggcat ccccggcgca
    27781 aaccacggca acaccagccg ctggagggaa gaccgcaggt aatgttggtt cgttgaaatc
    27841 ggcaccgtcc agccattaca ctctgcagct gagcagttcc tctaactacg acaacctgaa
    27901 cggttgggcg aagaaagaga atctgaaaaa ctacgttgtc tatgaaacga cgcgtaatgg
    27961 tcagccgtgg tatgtcctgg tttctggcgt gtacgcttcg aaagaagagg cgaaaaaagc
    28021 ggtatctaca ttgccagcag atgttcaggc caaaaacccg tgggcgaaac cgctgcgtca
    28081 ggtacaggcc gatctgaagt aatcaaggtt atctcccgca atggtttatc gttgcgggag
    28141 ctgcctgaag cgctggatgc tgtcggagct ttctccacag ccggagaagg tgtaattagt
    28201 taatcggcat gaagaaaaat cgcgcttttt tgaagtgggc agggggcaag tatcccctgc
    28261 ttgatgatat taaacggcat ttgcccaagg gcgaatgtct ggttgagcct tttgtaggtg
    28321 ccgggtcggt gtttctcaac accgactttt ctcgttatat ccttgccgat atcaatagcg
    28381 acctgatcag tctctataac attgtgaaga tgcgtactga tgagtacgta caggccgcac
    28441 gcgagctgtt tgttcccgaa acaaattgcg ccgaggttta ctatcagttc cgcgaagagt
    28501 tcaacaaaag ccaggatccg ttccgtcggg cggtactgtt tttatatttg aaccgctacg
    28561 gttacaacgg cctgtgtcgt tacaatctgc gcggtgagtt taacgtgccg tttggccgct
    28621 acaaaaaacc ctatttcccg gaagcagagt tgtatcactt cgctgaaaaa gcgcagaatg
    28681 cctttttcta ttgtgagtct tacgccgata gcatggagcg cgcagatgat gcatccgtcg
    28741 tctattgcga tccgccttat gcaccgctgt ctgcgaccgc caactttacg gcgtatcaca
    28801 caaacagttt tacgcttgaa caacaagcgc atctggcgga gatcgccgaa ggtctggttg
    28861 agcgccatat tccagtgctg atctccaatc acgatacgat gttaacgcgt gagtggtatc
    28921 agcgcgcaaa attgcatgtc gtcaaagttc gacgcagtat aagcagcaac ggcggcacac
    28981 gtaaaaaggt ggacgaactg ctggctttgt acaaaccagg agtcgtttca cccgcgaaaa
    29041 aataattctc aaggagaagc ggatgaaaca gtatttgatt gccccctcaa ttctgtcggc
    29101 tgattttgcc cgcctgggtg aagataccgc aaaagccctg gcagctggcg ctgatgtcgt
    29161 gcattttgac gtcatggata accactatgt tcccaatctg acgattgggc caatggtgct
    29221 gaaatccttg cgtaactacg gcattaccgc ccctatcgac gtacatctga tggtgaaacc
    29281 cgtcgatcgc attgtgcctg atttcgctgc cgctggtgcc agcatcatta cctttcatcc
    29341 agaagcctcc gagcatgttg accgcacgct gcaactgatt aaagaaaatg gctgtaaagc
    29401 gggtctggta tttaacccgg cgacacctct gagctatctg gattacgtga tggataagct
    29461 ggatgtgatc ctgctgatgt ccgtcaaccc tggtttcggc ggtcagtctt tcattcctca
    29521 aacactggat aaactgcgcg aagtacgtcg ccgtatcgac gagtctggct ttgacattcg
    29581 tctggaagtg gacggtggcg tgaaggtgaa caacattggc gaaatcgctg cggcgggcgc
    29641 ggatatgttc gtcgccggtt cggcaatctt cgaccagcca gactataaaa aagtcattga
    29701 tgaaatgcgc agtgaactgg caaaggtaag tcatgaataa gtttgaagat attcgcggcg
    29761 tcgcttttga tcttgatggt acgctggtcg acagtgctcc tggtcttgct gctgcggtag
    29821 atatggcgct gtatgcgctg gagttgcccg tcgcaggtga agaacgcgtt attacctgga
    29881 ttggtaacgg cgcagatgtt ctgatggagc gcgcattgac ctgggcgcgt caggaacgtg
    29941 cgactctgcg taaaacaatg gggaaaccgc ccgttgatga cgacattccg gcagaagaac
    30001 aggtacgtat tctgcgtaaa ctgttcgatc gctactatgg cgaggttgcc gaagagggga
    30061 cgtttttgtt cccgcacgtt gccgatacgt tgggcgcgtt gcaggctaaa ggcctgccgc
    30121 taggcttggt caccaacaaa ccgacgccgt tcgtcgcgcc gctgctcgaa gccttagata
    30181 ttgccaaata ctttagcgtg gttatcggcg gcgatgatgt gcaaaacaaa aaaccgcatc
    30241 cggacccgct gttactggtg gctgagcgga tgggaattgc cccacaacag atgctgtttg
    30301 tcggcgactc acggaatgat attcaggcgg caaaagcggc aggttgccca tcagttggct
    30361 taacctacgg atacaactac ggcgaggcta tcgatctcag ccagcctgat gtaatttatc
    30421 agtctataaa tgaccttctg cccgcattag ggcttccgca tagcgaaaat caggaatcga
    30481 aaaatgacta agcccatcgt ttttagtggc gcacagccct caggtgaatt gaccattggt
    30541 aactacatgg gtgcgctgcg tcagtgggta aacatgcagg atgactacca ttgcatttac
    30601 tgtatcgttg accaacacgc gatcaccgtg cgccaggatg cacagaagct gcgtaaagcg
    30661 acgttggata cgctggcctt gtatctggct tgtggtatcg atcctgagaa aagcaccatt
    30721 tttgttcagt cccacgtacc agaacatgcg cagttaggct gggcactgaa ctgctatacc
    30781 tacttcggcg aactgagtcg catgacgcag tttaaagata aatctgcgcg ttatgccgag
    30841 aacatcaacg ctggtctgtt tggctatccg gtgctgatgg ctgcggacat cctgctgtat
    30901 caaactaatc tggtaccggt gggtgaagac cagaaacagc acctggaact gagtcgcgat
    30961 atcgcccagc gtttcaacgc gctgtatggc gagatcttta aggtgccgga gccgtttatt
    31021 ccgaaatctg gcgcgcgcgt aatgtcgctg ctggagccga ccaagaagat gtccaagtct
    31081 gacgataacc gcaataacgt tatcggcctg ctggaagatc cgaaatcggt agtgaagaaa
    31141 atcaaacgcg cggtcactga ctccgacgag ccgccggtag ttcgctacga tgtgcagaac
    31201 aaagcgggcg tttccaacct gctggatatc ctttctgcgg taacgggcca gagcatccca
    31261 gaactggaaa aacagttcga aggcaagatg tatggtcatc tgaaaggtga agtggctgat
    31321 gccgtttccg gtatgctgac tgaattgcag gaacgctatc accgtttccg caacgatgaa
    31381 gccttcctgc aacaggtgat gaaagacggc gcagaaaaag ccagtgtaca cgcttcccgt
    31441 acgctgaaag cggtgtacga agcgattggt tttgtggcga agccgtaata tcaaaacgct
    31501 cggacggtcc cctcgcccct ttggggagag ggttagggtg aggggagcag gcaagcgcca
    31561 gtgggaatta ccctttgctc gcattgtcga ataaatcact tcgtcatcac tctgaaaccg
    31621 gggaaacccg gtttttttat cctcttttcg caacaacccg aaaaaatgtg aagcgcctcg
    31681 ccgtttccac atctcgtcgt cgccacttat cttcatttta atgaagatat aaatattcat
    31741 tttattgaaa atatattatg cgtcgaacgt ttatcaaaaa agaaggcgtc gtcatcacga
    31801 cactggcccg ttatttgttg ggtgaaaaat gcggtaatcg actgaaaacc atagatgagc
    31861 tggcaactga atgccgttca tcggttggcc tgacgcaggc cgcgttgaaa acgctggaat
    31921 caagcggagc gatacggatt gaacgccgtg ggcgcaatgg cagttatctt gtcgagatgg
    31981 ataacaaagc attgctgagt catgtggata tcaacaacgt ggtatgtgcg atgcctttgc
    32041 cctatacccg cttgtacgaa ggtttagcga gcggattgaa agcccagttt gatggcattc
    32101 ctttttacta tgcgcatatg cgcggcgcgg atattcgcgt ggagtgttta cttaacggtg
    32161 tctatgacat ggcagtagtt tcccgcctgg cggcggaaag ttatctcagc caaaacaatt
    32221 tgtgcatcgc cctcgagctt ggcccgcata cgtatgttgg tgaacatcaa ctgatatgcc
    32281 gcaaaggtga gtctggaaat gttaagcgag taggtctcga tagtcgttcg gcggatcaga
    32341 aaatcatgac cgatgtcttt tttggcgata gtgatgtgga acgagtcgat ctctcttatc
    32401 acgagagttt acaacgcatt gttaaaggcg atgtcgatgc ggtgatctgg aacgtggtgg
    32461 cggaaaacga actgaccatg ctgggattag aggcgacgcc gctaacagac gatccgcgat
    32521 ttttacaggc caccgaagcc gtggtcctga cgcgagtcga tgattaccca atgcaacaat
    32581 tgctgcgtgc tgttgtagat aaacacgccc tgcttgccca tcaacaacgg gtggtgagtg
    32641 gggaacagga acccagttat taaacgaaag gcatctgata tggaaaccag actcaacctg
    32701 ctttgcgagg caggcgttat tgataaggac gtctgcaaag gcatgatgca ggtcgtcaac
    32761 gtactggaaa aagagtgcca tctgccggtg cgcagtgagc aaggaacgat ggcgatgaca
    32821 catatggcga gtgcactgat gcgcagtcgc cgtggtgaag aaatagagcc gctggataac
    32881 gagttgctgg cggaactggc gcaatccagc cactggcaag ccgttgtgca attgcatcag
    32941 gagttgttga aggaattcgc tctggaagtt aacccgtgtg aagaaggcta ttttctggcc
    33001 aatctttatg gattatggat ggctgctaac gaagaggttt gaagctcccg acaaatccac
    33061 gtcgcacaac ctaaatattc aaaaaaatga atatttaatt caaggaacag aaacgaggca
    33121 acaagatgtt tgtagaagca ttgaaacgcc agaacccggc gctgatttcc gccgcactaa
    33181 gtttgtggca gcagggcaaa atcgccccgg acagctgggt gatcgacgtg gatcagatac
    33241 tggaaaacgg taagcggctc attgagacgg cgcggcttta cggcattgaa ctgtatctga
    33301 tgaccaagca atttggtcgt aatccgtggc tggcggaaaa attgctggca ttaggctaca
    33361 gcggcattgt ggcggtggat tacaaagagg cgcgagtcat gcgccgcgct ggtttgccag
    33421 tggcgcatca ggggcatctg gtacaaatcc cttgtcatca ggttgctgac gccgttgaac
    33481 agggcaccga catcatcacc gtgtttactc tcgacaaagc gcgggaagtt tctgcggcag
    33541 cggttaaggc cggacgggtc cagcctgtgc tgctcaaagt ttatagcgac gatgattttc
    33601 tttatccggg gcaggagagc ggttttgtac agcattcgct gcacgaggtg attgctgaaa
    33661 ttaagaaact gccggggctg catttagccg gactaaccca tttcccttgc ctgctttggg
    33721 atgaggctgc tgggaaagtt ttgccaacac cgaatcttca cacgctggta caagcacggg
    33781 atcaactggc gaaatctggt attgcgattg agcaactgaa cgtaccttca gcgaccagct
    33841 gcacttcgct gccattgctg gcggaatacg gtgcgactca tgccgaaccc ggtcatgcgc
    33901 tgaccggtac tattccggca aaccagcagg gcgatcagcc tgaacgtatc gcgatgctct
    33961 ggttaagtga aatctcccat catttccgtg gcgacagcta ctgctacggc ggcggttact
    34021 accgtcgtgg tcatgcgcaa catgcgctgg tgtttacgcc agaaaatcaa aagattactg
    34081 aaaccaatct caaaactgtg gatgacagca gtatcgacta caccctgccg ctggcaggcg
    34141 agtatccggt gagcagtgca gtggtgctct gttttcgcac gcagattttt gtcacccgta
    34201 gcgatgtggt gctggtgtcc ggtattcagc gcggcgaacc agaaatagtt ggtcgttatg
    34261 acagcctcgg caaccctctg gaggcataaa tggcgcgatt tgtggtgtta gtgattgata
    34321 gctttggcgt aggggcaatg aaagatgtca cgctggtgcg cccgcaagat gccggggcga
    34381 atacctgtgg tcacatcctg agccagttgc cgcatttgca gctaccaacg ctggagaagc
    34441 tggggctaat caacacattg ggttatgcgc caggcgatat gcagccgtca gattccgcaa
    34501 cctggggcgt ggcagagctg caacatgaag gtggcgatac ctttatgggg catcaggaaa
    34561 ttttaggcac gcgcccgtta ccgccgctgc ggatgccttt tcgcgatgtg attgaccgtg
    34621 ttgagcaggc attagtttcc gctggctggc aggtggagcg ccgtggcgat gatctgcaat
    34681 ttctgtcggt caatcaggcg gttgcgattg gcgataatct cgaggcggat ttaggccaag
    34741 tctataacat taccgccaat ctctctgtga tctcttttga cgacgcaatc aaaattggtc
    34801 gtatcgtgcg tgaccaggta caggtcggtc gggtcattac atttggcggc ctgttaacca
    34861 acagtcagcg tattctcgat gccgcagaaa gcaaagaagg gcgctttatt ggtattaatg
    34921 cgccgcgttc tggcgcttat gacaacggct tccaggtcgt gcatatgggc tacggtgttg
    34981 atgaaaaagt gcaggtgccg caaaaactgt atgaagcggg cgtgccaacc gtgctggtgg
    35041 gtaaggtggc agatatcgtc agcaatcctt atggtgtgag ctggcaaaac ctggtggata
    35101 gccagcggat tatggatatc accctcgacg aatttaacac ccatccgacg gcgtttattt
    35161 gcatcaatat ccaggaaacc gacctcgctg gtcatgcaga agacgtcgca cgttatgccg
    35221 aacgtttgca ggtcgttgac cgtaaccttg cccggcttgt tgaggcgatg cagccagatg
    35281 attgcctggt cgtgatggcg gatcacggta acgatccgac cattggtcac agccaccata
    35341 cccgtgaagt ggtgccagtg ctggtttatc agcaagggct ggtcgccacg cagctcggtg
    35401 tgcgcaccac gctttctgat gtgggagcta ccgtgtgtga atttttccgt gcgccaccgc
    35461 cacaaaatgg tcgctctttt ctttcctccc tccggtttgc aggagacacc ctatgagttt
    35521 tgatccgacg ggttacaccc tggcccatga gcatctgcat attgatctct ccggctttaa
    35581 aaacaacgtg gactgccgcc ttgatcagta tgcgttcatt tgccaggaga tgaacgacct
    35641 gatgacccgg ggtgtacgta atgtgattga gatgaccaac cgttacatgg ggcgcaatgc
    35701 gcaatttatg ctcgatgtaa tgcgcgaaac cgggatcaac gtggtggcct gtactggtta
    35761 ttaccaggat gcgtttttcc cggaacatgt ggtgacccgc agcgtgcagg aactggcgca
    35821 ggagatggtc gatgaaattg aacagggtat cgatggcacg gagctgaaag ccgggatcat
    35881 cgcggagatc ggctccagcg aaggaaagat tacgccgctg gaagagaagg tatttattgc
    35941 cgctgcgctg gcgcataacc agaccggacg cccgatctcc acgcatacgt cgttcagcac
    36001 catggggctg gaacaactgg ctctgttgca agcccacgga gttgatcttt cgcgcgtcac
    36061 cgttggtcac tgcgatctga aagacaacct cgacaacatt ttgaagatga tcgatctcgg
    36121 cgcgtacgtg cagttcgaca ccattggtaa gaacagttac tacccggacg aaaagcgcat
    36181 tgcgatgctc cacgcgctac gtgaccgtgg gttgctgaac cgcgtcatgc tgtcgatgga
    36241 tattacgcgc cgctcccatt taaaagccaa tggtggttat ggctatgact atttattgac
    36301 cacctttatt ccgcaattgc gccagtcagg attcagtcag gccgatgtgg atgtgatgtt
    36361 acgtgaaaat ccctctcaat ttttccaata aggacagact catgaaaaag attggcgttg
    36421 caggcttaca gcgtgagcag attaaaaaaa ccattgaagc gacggctcct ggctgttttg
    36481 aagttttcat tcacaacgac atggaagcgg caatgaaggt gaaatccggg caactagatt
    36541 attacattgg cgcgtgtaat accggtgcgg gcgcggcatt gtcgattgcc atcgcggtga
    36601 ttggctataa caaaagttgc accattgcca aaccaggcat taaagcgaaa gacgagcata
    36661 tcgccaaaat gatcgctgaa gggaaagtgg cgtttggcct ttccgttgag cacgtcgaac
    36721 acgcgattcc gatgttgatt aaccatctga aataaaaggc acgactatgg atctgtatat
    36781 tcagattatc gtggtggcgt gcctgacggg tatgacatcg cttctggcgc atcgctcggc
    36841 ggctgttttt catgacggca tccacccg
//
LOCUS       DADLTY010000015        36655 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.015, whole genome shotgun sequence.
ACCESSION   DADLTY010000015 DADLTY010000000
VERSION     DADLTY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.015"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(164..955)
                     /gene="cpoB"
                     /locus_tag="JN334_000662"
     CDS             complement(164..955)
                     /gene="cpoB"
                     /locus_tag="JN334_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HAY7436915.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(965..1486)
                     /gene="pal"
                     /locus_tag="JN334_000663"
     CDS             complement(965..1486)
                     /gene="pal"
                     /locus_tag="JN334_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAY7436916.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1521..2813)
                     /gene="tolB"
                     /locus_tag="JN334_000664"
     CDS             complement(1521..2813)
                     /gene="tolB"
                     /locus_tag="JN334_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HAY7436917.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(2946..4187)
                     /gene="tolA"
                     /locus_tag="JN334_000665"
     CDS             complement(2946..4187)
                     /gene="tolA"
                     /locus_tag="JN334_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAY7436918.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4252..4680)
                     /gene="tolR"
                     /locus_tag="JN334_000666"
     CDS             complement(4252..4680)
                     /gene="tolR"
                     /locus_tag="JN334_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HAY7436919.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4684..5376)
                     /gene="tolQ"
                     /locus_tag="JN334_000667"
     CDS             complement(4684..5376)
                     /gene="tolQ"
                     /locus_tag="JN334_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HAY7436920.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5373..5777)
                     /gene="ybgC"
                     /locus_tag="JN334_000668"
     CDS             complement(5373..5777)
                     /gene="ybgC"
                     /locus_tag="JN334_000668"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY7436921.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(5927..6220)
                     /gene="ybgE"
                     /locus_tag="JN334_000669"
     CDS             complement(5927..6220)
                     /gene="ybgE"
                     /locus_tag="JN334_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HAY7436922.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6220..6333)
                     /gene="cydX"
                     /locus_tag="JN334_000670"
     CDS             complement(6220..6333)
                     /gene="cydX"
                     /locus_tag="JN334_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAY7436923.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6348..7487)
                     /gene="cydB"
                     /locus_tag="JN334_000671"
     CDS             complement(6348..7487)
                     /gene="cydB"
                     /locus_tag="JN334_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY7436924.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7503..9071)
                     /gene="cydA"
                     /locus_tag="JN334_000672"
     CDS             complement(7503..9071)
                     /gene="cydA"
                     /locus_tag="JN334_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAY7436925.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(9920..12553)
                     /gene="mngB"
                     /locus_tag="JN334_000673"
     CDS             complement(9920..12553)
                     /gene="mngB"
                     /locus_tag="JN334_000673"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HAY7436926.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(12571..14547)
                     /gene="mngA"
                     /locus_tag="JN334_000674"
     CDS             complement(12571..14547)
                     /gene="mngA"
                     /locus_tag="JN334_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HAY7436927.1"
                     /translation="MVLFCRAHWRDYKNDQVRIMMNLTTLTHRDALCLNARFTSREEA
                     IHALTQRLAALGKISSTEQFLKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVAT
                     LSEPLQWEGVDGPEAVDLVVLLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATT
                     PDELLSALDDKGGTQPSASFSNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVY
                     VEKQGANGIEGRLTADQLNSATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEAL
                     IQQSLTLERGDETRTLQQDTQPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLL
                     SQIFGLQDLFNEENSWLWMYRKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGL
                     AANMIGSGFLGAVVGGLIAGYLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLML
                     FVVGEPVAWINNSLTAWLNGLSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMA
                     NGVYGPYAIFASVKMVSAFTVTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPM
                     AIEDPLRVIGSFVLGSMVTGAIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWF
                     GAALVGAAISTAILLIWRRHAVKHGNYLTDGVMP"
     gene            14657..15379
                     /gene="mngR"
                     /locus_tag="JN334_000675"
     CDS             14657..15379
                     /gene="mngR"
                     /locus_tag="JN334_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HAY7436928.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15482..16351)
                     /gene="sucD"
                     /locus_tag="JN334_000676"
     CDS             complement(15482..16351)
                     /gene="sucD"
                     /locus_tag="JN334_000676"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAY7436929.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16351..17517)
                     /gene="sucC"
                     /locus_tag="JN334_000677"
     CDS             complement(16351..17517)
                     /gene="sucC"
                     /locus_tag="JN334_000677"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAY7436930.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17611..18828)
                     /gene="odhB"
                     /locus_tag="JN334_000678"
     CDS             complement(17611..18828)
                     /gene="odhB"
                     /locus_tag="JN334_000678"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAY7436931.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(18843..21644)
                     /gene="sucA"
                     /locus_tag="JN334_000679"
     CDS             complement(18843..21644)
                     /gene="sucA"
                     /locus_tag="JN334_000679"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAY7436932.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(21945..22661)
                     /locus_tag="JN334_000680"
     CDS             complement(21945..22661)
                     /locus_tag="JN334_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAY7436933.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(22677..24443)
                     /gene="sdhA"
                     /locus_tag="JN334_000681"
     CDS             complement(22677..24443)
                     /gene="sdhA"
                     /locus_tag="JN334_000681"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAY7436934.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24443..24790)
                     /gene="sdhD"
                     /locus_tag="JN334_000682"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24443..24790)
                     /gene="sdhD"
                     /locus_tag="JN334_000682"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HAY7436935.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(24784..25188)
                     /gene="sdhC"
                     /locus_tag="JN334_000683"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(24784..25188)
                     /gene="sdhC"
                     /locus_tag="JN334_000683"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY7436936.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25569..25676
                     /locus_tag="JN334_000684"
     CDS             25569..25676
                     /locus_tag="JN334_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436937.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            25882..27165
                     /gene="gltA"
                     /locus_tag="JN334_000685"
     CDS             25882..27165
                     /gene="gltA"
                     /locus_tag="JN334_000685"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAY7436938.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            27556..28122
                     /locus_tag="JN334_000686"
     CDS             27556..28122
                     /locus_tag="JN334_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7436939.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28173..30632
                     /locus_tag="JN334_000687"
     CDS             28173..30632
                     /locus_tag="JN334_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7436940.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            30647..31378
                     /locus_tag="JN334_000688"
     CDS             30647..31378
                     /locus_tag="JN334_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7436941.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31375..32451
                     /locus_tag="JN334_000689"
                     /pseudo
     CDS             31375..32451
                     /locus_tag="JN334_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            32558..33649
                     /locus_tag="JN334_000690"
     CDS             32558..33649
                     /locus_tag="JN334_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAY7436942.1"
                     /translation="MLYRYTKEKIMRERRMPVLQWGMLCVLSLLLSIGFLAVHLPAAL
                     LLGPMIAGIIFSMRGITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAI
                     LLVTLLSSAIVGWLLVRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYL
                     RVLFVAGAAVLVTRMMLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLP
                     SGTMLIPMLAGAVLQSGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQ
                     ILLSIFALLAICAGMAWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMA
                     MQTLRLFSILLTGPAIARFISTYAPKRSA"
     gene            complement(33646..34437)
                     /gene="nei"
                     /locus_tag="JN334_000691"
     CDS             complement(33646..34437)
                     /gene="nei"
                     /locus_tag="JN334_000691"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HAY7436943.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34473..35207)
                     /gene="pxpA"
                     /locus_tag="JN334_000692"
     CDS             complement(34473..35207)
                     /gene="pxpA"
                     /locus_tag="JN334_000692"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HAY7436944.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35197..36129)
                     /gene="pxpC"
                     /locus_tag="JN334_000693"
                     /pseudo
     CDS             complement(35197..36129)
                     /gene="pxpC"
                     /locus_tag="JN334_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(36123..>36655)
                     /gene="pxpB"
                     /locus_tag="JN334_000694"
     CDS             complement(36123..>36655)
                     /gene="pxpB"
                     /locus_tag="JN334_000694"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAY7436945.1"
                     /translation="VVEAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESR
                     FIEIPVVYGGAGGPDLAVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPE
                     QLHTPRRAEPRLLVPAGSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILL
                     RPGDSVRFVPQKEGIC"
BASE COUNT         8940 a         9865 c         8894 g         8956 t
ORIGIN      
        1 ctttttcgtt gctttcggtt tgtttgatat cccgtggcaa cggcggcata tattactgat
       61 ttcagacttg agcgcaacaa aaatttcgat gcagatcact caactgctta tgattcgcac
      121 gacacgacca gaaataatgc gacttctggt cgtgtgttat gcattacatc gcgttcagac
      181 gtttttgtgc ctgtttagcg ccatcagtac cagggtattt actgataacc tgctggtata
      241 cggctttcgc ttttgcggtg tcacctttgt cctgcatgat gacgccgact ttaaacatcg
      301 catcagcagc ctttggtgac ttcggatagt ttttcactac cgaagcaaaa tagtacgccg
      361 catcatcttt tttacccttg ttgtagttta actgacccag ccaataattg gcgtttggca
      421 ggtaagttga atcagggtaa tttttgatga aattctgaaa tgccaccatt gcgtcatcct
      481 ggcgggattt atcctgcacc agcgcaatag ctgcattgta atccgtgttt gcatcgccgc
      541 ttttcaccgg cgcgccagca ttcgcagtac cagcatcagc tgtcggcgtc gttgatgccg
      601 tcgcaccgct ttgatcgctg ctggttgatt gcgccgctgc accaccgctg ctgaggctgt
      661 cgatctgcaa caggatctgc ttttgccgct ccacgacctg attcagttga tactgatttt
      721 cctgaatctg accacgcagg gaatcaatat cggattgatt atcagaaagt tgttgctgga
      781 gttgggttaa aagctggctg tgagcattag aaatacgctc aagttgagtg acgcggtctt
      841 cgaccgagcc tgagctgaca ctactgattg gtgcctgagc aaaagcggcc cagggggccg
      901 ctataccaac cagtaacgac agactcaata gttgatgtct gaagttactg ctcatgcaat
      961 tctcttagta aaccagtacc gcacgacggt ttttggagta tgccgcttcg tcatgaccca
     1021 gtactgcagg tttttcttta ccgtaagaaa cgatggagat ctggtctgca gaaacgcctt
     1081 taccctgcag gtacatctta acggcgttcg cacgacgttc acccagggag atgttgtatt
     1141 ccggagtacc acgttcgtcc gcgtgacctt ctacggtgac tttgtaagac gggttgctac
     1201 gcaggaagtt tgcatgtgca tccagcattt gagcgaagtc agaacggata tcgtacttgt
     1261 ccagatcgaa gtaaacgatg ttgttctgct gcagctgttg catttgcaga cgagcctgct
     1321 cttcggaaga catgttgccg ttgccgccgt tcgcatccat accagtgccg gcacccagca
     1381 tgccttcgct gccgtcattg ctggcgttct tgttggaaga acatgccgca attgccataa
     1441 caggcagagc aatcatcagc cctttcagca ctttgttcag ttgcatttca atgattcctt
     1501 tactattcaa ttaattatta tcacagatac ggcgaccagg cagggaattt gacctgtcca
     1561 tcagttgccg gaagacgcgc tttgaaacgc ccatctgtag aaaccaaatt cagcacggat
     1621 cccatcccct gagaagagct gtagattacc atagtgccgt taggtgccag acttggcgtt
     1681 tcatccagga acgtggacga cagaacttgt acgcctcccg ttgccagatc ttgtttggca
     1741 atgtgctgct gcccaccatt ggagctgacc attaccataa atttaccgtc gctgctgaca
     1801 tccgcatcct ggttctgcga accttcccag gtaatacgtt gtggcgcacc gccgttgata
     1861 ttcactttat aaacctgcgg acgaccggcc tggtcagaag taaatgccag gttctggcta
     1921 tccgggaacc aggtcggttc ggtattgtta ctgcgaccat cagtcacctg gcggatctga
     1981 ccagaagcca aatccattac gtacaggttc agactaccgg ttttcgacaa ggcgaatgcc
     2041 agtttgctgc cgtctggcga gaatgcaggt gcaccgttgt gacgcgggaa tgaagccacc
     2101 tgacgtacag cgccatttgc cagcgtctga ataaccagcg cggaacgacc gctttcgaag
     2161 gtcacataag ccagtttaga accgtctggt gaccacgccg gagacatcag cggctgcggt
     2221 gaacggtgaa cgacaaactg gttgtaaccg tcatagtcag atacgcgcag ttcatacggg
     2281 aactgaccgc cgttggtctg aacaacgtag gcaatacggg tacggaacgc acctttaatg
     2341 ccggtcagtt tttcaaacac ttcatcactg gcggtatgac cagcataacg cagccactgc
     2401 ttgttcactt tgtacgagtt ctgagcaagt acagtacccg gtgcgccgcc agtgtcaaca
     2461 agttgataag caacattgta agaaccatcc ggattcggag tgacttgacc gacaactaca
     2521 gcgtcaatgc ccagtgcgga ccatgcagct ggttgtactt cctgcgcact acccggctgc
     2581 tgtggcagac gagcgcgatc taacggatta aatttaccgc tgttacgcaa gtcagcagca
     2641 acgatgccgc caatatcttc aggtgccgca ccaggccccg cccactggaa aggaacaaca
     2701 ccaataggac gaccggaatc tacaccgctg tcgatcacaa tgcggacttc agcatgcaga
     2761 actgatgccc acagtatgag aaaaccaaat gctactcgta atgcctgctt catcatatct
     2821 cccttatctg gaccgatggc ccacgataat ttagcagaat gttaacaaac taaaatacac
     2881 aaaactacca gaaccccgtg gcaaccggtg cctgatgttg accgtccgaa cagtcaacat
     2941 cgcgattacg gtttgaagtc caatggcgcg tttttgaaca cttcatatac tgcctggctt
     3001 ggtggtttcg ggatcttcgc aagtttagct gctgccaacg cagcctgaca aagtgcggga
     3061 tcgccacctt caggtttgat atccagtaac ataccatcgg gtgccagttt tatgcgcagc
     3121 gtacaggttt tgcctgcata ggacgatgcg tcatagaact tactttcgat agcagattta
     3181 atctgcccgg catagttatt gatatcggcc cctgatgcgc cattgttttt agtattacca
     3241 ctcccggcag gcgaagcatt gttccctttc gcccctcccc ccgttttcgg tgcattctta
     3301 ccagagctta gctcaccgaa aatatcatct gcctctgcgg cagcttttgc tgcagcggct
     3361 ttttcagctg ctgcttttgc cgctgccgct tttttgtctg cggcggcttt ttctgccgct
     3421 gctttcttat cagctgctgc cttatcagca gccgcttttt ctgctgcttt tttgtcggct
     3481 gcagctttct ctgctgccgc tttcttatca gctgcagcct tttcagcagc cgctttcttc
     3541 tcagcttctg ccttggcttt ttcagcagct tcagttgccg ctttctttct tgcttcagct
     3601 gcagctgctt ctgccgcttc cgctttcttc ttcagtgccg cagcggctac ctcggctttt
     3661 ttctgcgctt cggctgcggc tttggcggct tctgcttctg cttttttctt cgcgtctgca
     3721 gccgctttct tcgctgcttc ttccgcagct ttagcatctg ccgccgcttt cgctgccgcc
     3781 acttcagctt gcttctgctt taactcggcc tgttttgcgg cttcttcagc ctgctttttc
     3841 tgctcctgag ccgctaaccg ctctttctca agttgcttca ggcgttcctg ttcagccgct
     3901 tgtttctcac gtagttcttc agcagcctgc tgttccttca tcttgcgctg ttcatcagaa
     3961 cgcttcgcgc ttgattcctg gctttgcatg cgtttgtact gctcaactac cgcacctgaa
     4021 tcaaccatga cagcgtcgat ggacgaacca ccgccgcctc cggctgaagc ttctatattc
     4081 tcatcgaacg aactccagat cagcgccgca aataagatga catgcagcac tgctgaaata
     4141 attatcgccc gcttgagctt gtcgttttgt tcggttgcct ttgacactct cggtttccaa
     4201 aaactgttcg cctgttaccc gctctctttc aagcaaggga aacgcagatg tttagatagg
     4261 ctgcgtcatt aaaccaaccg atttcacacc cgcactatgt aacaagttca gtgctttaat
     4321 tatttcatcg taaggcacat cttttgcgcc accgatcaga aagaccgttt tcgggttggc
     4381 cttgaaacgg ctggacactt ccgccaccac ctgctctggt ggtaaacgct ccaggcgatc
     4441 tttctcaacc accacggtgt actgaccaat accagacact tcaacaatca ctggcggatt
     4501 atcgttactg ctcaccgcct gtgattcagt agcgtctggc agatcgacct ccacgctctg
     4561 ggtgatgatg ggcgctgtcg ccataaagat cagcaacagc accagcagta cgtccagcaa
     4621 cggaacaatg ttgatttcgg acttgagatc gcgacgacct cgtccacgcg ctctggccat
     4681 ggcttacccc ttgttgctct cgctaacggt aaacgcctgg cggtgcagaa tcgcggtaaa
     4741 ctcttccata aagttgtcgt aattcagttc cagtttgttt acgcgctggt tgagacggtt
     4801 ataagccatt accgccggaa ttgcggcgaa cagaccaatt gccgtcgcaa tcaacgcttc
     4861 tgcgataccg ggcgcaacca tttgcagtgt tgcttgtttt accgccccga gggcgataaa
     4921 ggcgtgcatg atcccccaga ccgtaccaaa cagaccaata tacgggctga tggagccaac
     4981 cgtaccgagg aacggaatgt gcgtttccag attttcaagt tcacggttca tggagatacg
     5041 catagcacgc gacgcccctt ccactacggc ttccggcgca tggctattgg cacgatgcag
     5101 gcgcacaaac tctttgaacc cgctgtaaaa gatttgttcc gaaccagtca gattatcccg
     5161 tttcccctga ctctcttgat agaggcgaga gagttcgatt ccagaccaga atttatcttc
     5221 aaacgcttcg gcttcacgcg ccgctgcgtt aagaatacgg gttcgctgga taataatggc
     5281 ccaggatgcg attgaaaaac caatcaaaat caacatgata agtttaacca gaaggctagc
     5341 cttcaggaac aaatcaagga tattcatgtc agtcactgct taaactccgc gacaatagac
     5401 ttgggaagcg cacgaggctt cattttgagt gggtcaacgc aaacaaccag aacctctgct
     5461 tcattcagca aagtattctc ggcgttgaca atacgttgcg tgaaaaccaa agaggtgcca
     5521 cgcattgatg ttatttcagt ctgtatttcg agcatatcgt cgagccgcgc aggtgcgtaa
     5581 tattccaccg tcattttacg taccacaaag gcaacgcgtt cagccatcag cgcctgctga
     5641 ctgaagtgat gatgacgcag catctctgtg cgtgctcttt cataaaaagc gacgtaactg
     5701 gcgtggtaca ccacaccacc ggcatcggta tcttcatagt agacgcgaac cggccatcga
     5761 aacagcgttg tattcacttt acatcccggt aatgcaacaa aagttagagc ttttaaacgc
     5821 tgctactata cgcgcgcgat tgaggtttgg gaatgagggc aagttaaggg cgcgtaaaaa
     5881 tttatgggcc tagagcggcc cataattttt taaagagaat tctgatttaa aagaagaaga
     5941 aaatcagccc gacaatgaga acaatatcgg caagcaatgg gcaaaaaatc ccttgccaaa
     6001 gaaccttctg cggacgaaag cccacgccgt gaatcacacc agcacagacg gcccacatca
     6061 gcaataaacc atgccagatt tccagttcac tggtcttcgc ggcaaaacga gatgggtccc
     6121 aaaacataca tcctgctaac agaagcgcca tcacgaagga aagcgcccgc agggggcgct
     6181 tgtccattac cgcatacaag gtcgcgataa tcttactcat cagatgtctt cttgaccggc
     6241 tttgcctgat tcgacgtgtt caagcgccag cgcggtgatt accccaaacg aacaggcaag
     6301 aagcgttccc agaatccatg cgaaatacca catttttagc tccttactta gtacagagag
     6361 tgggtgttac gttcaatatc ttctttggtg atacgaccga acattttcca gtaacaccag
     6421 gcggtgtaga gcagaatgat cggtaccaga accaccgcaa cccaggtcat gacgttaagc
     6481 gtcagctggc tggaagttgc atcccacatt gtcagacttg cgttcatcat ggtgctggac
     6541 ggcatcacaa acgggaacat tgcgataccg gctgtcagga tgatgcaggc cagcgtcagg
     6601 gaggagaaca caaacgccca cgcggcttta tccatacgtg cagtcaggat ggtcagcagc
     6661 agcagaacca cacccagtgc cggaatagcc cacagaattg gcgtgttgtt gaagttaacc
     6721 agccatgcgc cagcttcacg aaccacttct ttattcagtg ggttagaggc tgcgtaatgg
     6781 tccattgtcg atttcacgac ataaccatcg ataccgtaca tcacccatac gccagccagt
     6841 gcgaaacaga ccagtgtcac cagcgcagcc acctgagccg ttgcacgggt acgcaggtgc
     6901 agttcgccca cggtacgcat ttgcagatag gttgcgccct gagtaatgat catccctacg
     6961 ctcaccacgc ctgccagcag gccgaacggg ttaagcaact ggaagaagtt accggtgtag
     7021 tacagacgca gatattcatc aacgttgaac ggtacgccct gcaacaggtt accgaacgct
     7081 acaccaatta ccagcggcgg aacgaagcta ccaatgaaga tgccccagtc ccacatgtta
     7141 cgccagcggg tttcttcaat cttggagcgg tagtcaaaac cgaccggacg gaagaacaaa
     7201 gacgccagca cgaggatcat cgccacatag aagccggaga acgcagcggc atagaccatc
     7261 ggccaggcag caaagagtgc gccgcccgcg gtgatcagcc aaacctggtt accgtcccag
     7321 tgtggtgcaa tggagttaat cataattcga cgctcggtgt cgttacgacc gaggaaacgg
     7381 gtgagcatgc ccacccccat gtcgaaaccg tcagtgactg caaaaccaat cagcagaacg
     7441 ccaaccagca gccaccagat aaaacgcaat acttcataat cgatcatttg acgactcctg
     7501 tcttagcgtg ccggctgagt agtcgtggaa gactgctcaa agtgatagcg accggttttc
     7561 aggctgcttg ggccgaggcg tgcaaacttg aacattaaga acaattctgc caccaggaac
     7621 agggtataca ggccgcaaat cagcaccatt gagaagatga gatcgcctgc ggtcagtgac
     7681 gaattcgcca cagctgtcgg cagcacttca ccgatagccc acggttggcg gccatattca
     7741 gccacgaacc agcccgcttc tacagcaatc cacggcagcg gaataccgta cagcgcggcg
     7801 cgcagaagcc attttttctc gccaatgcgg ttgcggatga cactccagaa agagagcgcg
     7861 atgattgcca gcagcaggaa gccacacgcc accatgatac ggaacgcaaa gtacagcggc
     7921 gctacacgcg ggatggagtc tttggttgcc tgttgaatct gcgcttcagt cgcatcagcc
     7981 acgtttggcg tatagcgttt cagcagcaga ccgtaaccga ggtctttctt catgctattg
     8041 aactggtcac gaaccgtctg gtcggtagaa ccagaacgca gttgttcgag cagagagtac
     8101 gccttcatcc cgttacgaat gcgttcttca tgctgcacca tcagctcttt caggccgata
     8161 accggggtat ccacggaacg cgttgcaatg atgcccagtg cgtaagggat ctgaatcgca
     8221 aatttgttcg tctcctcttc ctgatcagga atgccgaaca aagtaaaggc agcaggcgca
     8281 ggttgcgttt cccactcggc ttcaatagca gccagtttgg ttttctgcac gtcgcccatt
     8341 tcgtagccgg attcatcacc cagaacaata acagacagaa cagcagccat accgaagctg
     8401 gcagcgatag caaaggagcg tttagcgaag gcgaagtcac gacctttcag catataccat
     8461 gcgctgatac cgaggatgaa catcgcgcca gtcacataac cagacgctac agtgtgaacg
     8521 aatttcacct gagcaaccgg gttaagcacc agctcggaga agctcaccat ctccatacgc
     8581 atagtttcaa agttgaaatc ggacgcgatt gggttttgca tccagccgtt cgcaaccaga
     8641 atccacagtg cggacaggtt tgaaccgagc gccaccagcc aggtgacaca catatgctga
     8701 actttaccca gacgatccca accgaagaag aacagaccta caaaggtgga ttcgaggaag
     8761 aaggccatca gaccttcgat tgccagcggc gcaccgaaga tatcccctac atagtgggaa
     8821 tagtaagacc agttagtccc gaactggaac tccatggtca gaccggtagc cacacccaga
     8881 gcgaagttga taccaaacaa cttgccccag aacttggtca tatctttata aatctgtttg
     8941 ccggagagga cgtagaccgt ttccataatg gccaggagga acgccatacc gagcgtcagt
     9001 ggcacaaaaa ggaagtggta catcgcggtc aaggcaaact gtaagcgcga cagttcgact
     9061 atatctaaca tcatgactcc ttgctcatcg catgaagact ccgagagtga accccgttag
     9121 aaagggtcac acgcatgccc caatagaaat ttgtttgaac actttcatca taccatgtga
     9181 atgcacacaa aaacggcgta aagaaaggtt gcaaacatgt taataaaaac tcaaattgat
     9241 cccacgtata tattacgccg caaaatcctt acaataaaca ggtttttgtt gaagcaaatt
     9301 tgcatttttc gacggtgatc aatttatagc taaattgccg cctttcagcc aatttgatcg
     9361 agaacaattt atctcttttt gatgcccatt tccaagactt atacattgat aaatatcaat
     9421 ttctacaaat tatgttaatc atgtttcaac aaggtaactt tagtgatatt tatgtttaaa
     9481 atgtgtgaat tataagactg aaattagttt cggttggcaa ttaaagataa aaagagacga
     9541 agcaaaaaca gcacggttga ccttaattct ttttttggtc actggctaat acgacgcaga
     9601 gtttcaggat tattaataaa ggcataaatc tcccccccgc cgatattaac atttaattaa
     9661 cttataatta tattataatt aactcacccg tggttattat tttgttaaaa ataaagccac
     9721 atatacgtag cttgagagtg aagataaaca caaaattacc tgatgcgcta cgcttgccag
     9781 tgtctacatc atctctgcaa tatattgaat ttgcctgctt ttgtagaccg aataaagcgt
     9841 tcatgccgta tccggcatga acaaagcgcc ctttgtcaac aatctggccg cgcatatgtg
     9901 gcttcggttt gattgctatt caggcaagcc ggtaactgaa cgtccgtgac tggccgggta
     9961 aaaaaggccc cgatagattt gaaccttgat tttcctcggt ggtaatgtgt tcatccatca
    10021 tcgtttctga gcaagaaatc acctcgcgac tgaaagcaac agtcgcatca caggttgctg
    10081 attcagccgg attaaacagc cgtaaaatca cttcttgtcg gtcttcagct ttcttaagtg
    10141 cgcttatcag gcatcccact gggggcattt tcaacaaact ataactttcc ggcacgttga
    10201 atccggcttt gttgagcttc atcgcatccc atgggatttt gttgtaacac tgtactggag
    10261 tcagccatgc tcgcgcctgc tgagctacac cagcggcggt tggcgtaccg gtataactca
    10321 ataaacttag gcgacaagaa agcagaccac gtagttgtga gtccgggact ggcattttaa
    10381 ttcccgaagg ccgcctaggc cttaaaagca gatcttcttt ccccagtaag cccacgccgc
    10441 gcagcaacgt aatggcaaag gttttcttct cttcaccgat gacttcaaat tcacgtagcc
    10501 cttcgctaaa gacagccatg ccgttacgcc cttcctgtaa ggctgcatag ttgagcatat
    10561 tccataccgg aaccggcgct tctttccagc tttcttgctg ccagttgttc attgcactgt
    10621 cgttcacggg gcgcgttagc gaaccaaact gggtatctgc cagaacacta tcggtgttaa
    10681 aagatgtagg gatcaggacg cgaaggcgat gatcgtcagc ctggttatca aggttgatat
    10741 ccacatcaat gcgcctgttg ttatgactaa gagtgacaac caactcgaca tctactctgc
    10801 cagtggattg tcttgcgctg cgttgttgca gattacgcgg caccgccatt tcatagcgga
    10861 taacagccct gctctgccag gcctcatgaa taatctcgca ttgcggtttc gcattcgccg
    10921 aagtaattac ccactcttct tttgcgggtg aatagtcata ttcatcacca tcatcagagc
    10981 tttcttcaat ttgcaacacc cggtcatagc gcacaccgct atctttatct accagttgta
    11041 gcgttccatc ctcattgagc gcaatttgcc agaaagcatt ttccagtatc ccttcagcgt
    11101 cactttttgc cgcaatcacg ttgccaggct gattcgcttc gatataaagc gtgcgatagc
    11161 ccatagaagg gacaatctgg ttgatctgta tatcaaactc cataaaggga tcgtaattac
    11221 cgtaatgaac tatttgccga tcgattaggc ctggatcgat ctcacgggca tggcgaataa
    11281 aatacggtac aggctgaccg cgatcgtctc gtagattaaa ctggctggcg cgcagccgca
    11341 cagtggtgtt gatcacttct tcccgcggcc agggcatcag gttaaacagg acgagtttgt
    11401 cggcgtcgct ctgcggcatg ttgtcggcga ttttacgcat atagaaacgc agcagattat
    11461 ccgccatgtc ttcagccagt tcgaagcggg cgacgatttc gcgatgaact ttgtcacgac
    11521 agcagcagcc gatgctgtcg tgggcatgat tttttaagat ctctttccac attttctcca
    11581 gcaagccgtg gtggtatcca aaacccaacg tcctggccag cgttgccagc ggttccagca
    11641 gattaacaat cttattttca atacgtgcgt gggcaacttt gatatccatg cgcgtggaac
    11701 cgatggtgcg atgcacgcgc atatatttgc catcaataaa ttcccctttc agggttgcca
    11761 gattatctcg ctgcgcttcg atcttctcaa atacctcttc aaagcggctc atcacaaact
    11821 ttcgttgagg ataaatttcg cgcagtttct ccatcacttc gaagatattt tgctgcaatg
    11881 gcatctgatc atgcccattc ggcagcaaaa tctctttggt tacagacgct ttttccagca
    11941 cgtcaaaata actgtcgagg cgtttgcgta atccgttttc gtccgcaggt aagtacttac
    12001 cgatggcata acccaacggc atcacctgcg ccgtcacttc gctaccgtca ctgctttgcc
    12061 acaaaaactc ggttttatcc gtaccgtggc gctcagaaca tccacgccag aacatggtgc
    12121 aggtaatgcc aaatccattg tagatatgcg gcagttgccc ggacatgcca aaggaatcgg
    12181 gtaaataacc tattttcatc ggctcgccaa acgcgaggca gtcacgcatt ccgtacatca
    12241 gattacggac gatggattcc gcagaaacaa tcgtggtatc ggtctgggta taccaggggc
    12301 cgataatcaa cttgccggct tctaccagtt tcttcaagcg gtctttgttt tccggtttca
    12361 ccgcgaaata atcttcgagg atcgccgttt gcccgtcgag tacgtaatat ttgtattcgt
    12421 tgtcctgttc cagtcggcac aggatctctt ccatattatt gaccagcaga atgcgtgact
    12481 cttcggtggt gaaataccac tcccgatccc agtgcatatg cggggtgatg tgaacgcgag
    12541 ataccgcttt catcgtcgtt tcctttttgt ttatggcatt acgccatcag tcagatagtt
    12601 gccatgctta accgcgtgac gccgccagat caggagaatt gcagtcgaga ttgcagcccc
    12661 caccaatgcc gcaccaaacc agccaattgc cgccataaca ccgcccgcgc cattatcatg
    12721 aagtaaaaag agcgagaaaa tgccagcacc gggtgtcgaa aggccgatat tcatcgcacc
    12781 gacaatagcg cccgttacca tagagcccag cacaaacgaa ccaataaccc gcagcggatc
    12841 ttcaatcgcc atcgggatcg ccccttcggt aatacctgcc agccctaaca gccaggtgga
    12901 tttcccggtc tcaatttcaa actctttaaa caggcgcggt gcgagcatcg tggaagcggt
    12961 tacggtaaat gccgaaacca ttttgacgga ggcgaaaatg gcatacgggc cgtaaacgcc
    13021 gttcgccatt gcgcccaggc agaatgcata agcggcttta ttcactggcc ctccaaggtc
    13081 aaaggaacac ataaaaccga gaatggcacc cagcaacagc gcgttacttc ctgacagacc
    13141 gttcagccag gcggtaagcg agttattgat ccaggcgaca ggttccccca ccacaaacag
    13201 catcagactg cccgctccca atgtaccgag caccgggtag agataaaaag tcaggaagcc
    13261 attgaattta ctgctaagac gcaagtgatt tttcatccag cgtatcaagt aaccggctat
    13321 caatccgcca acgaccgcgc cgagaaaccc ggagccgatc atgttggcgg caagtccagc
    13381 cgcaaagcct ggcgctaacg ccggtttatc tgccagcgaa taggcggtat aggctgccag
    13441 caccggtacc atcaaaattc cgagcagccc gccgcccagt ttgcggtaca tccacagcca
    13501 ggagttttct tcattaaaca gatcttgtag cccgaagatt tgcgacagta ataccgcgac
    13561 cgccagcacc gtcccccccg cgacaatcaa cggtacggca aaagagatcc cgctcaacag
    13621 tgcctgtttc agctccgttt tgacgctttt caccggttgc gtatcttgct gtaaggtacg
    13681 cgtctcatcg ccacgctcga gggtaagcga ttgttggatc aacgcttctg catggcgaat
    13741 cggctcggca acaggcacag aaagcgcagg aatgccgtta aaacgctcac tctccttgat
    13801 ggcgacttca gccgcaaaaa tacaggcagt tgcactattg agttgatccg ccgttaaacg
    13861 cccttcaata ccgttagcac cttgcttttc aacgtagaca ttcacgccga gtttgcgtcc
    13921 ggctttttcc agatactccg cagccatgta ggtgtgagca atacccgccg ggcaggccgt
    13981 tacgcaaacg atagttggcg cgttggaaaa agaggcagaa ggttgcgtgc ctcccttgtc
    14041 atccagcgcc gagagcaatt catcaggcgt cgtcgccgac tgtatacgcg cccgaatctc
    14101 atcatccgcc aggcgcgtgg tcagcgctgt cagcagttgc atgtgcgtag tacccgcttc
    14161 attgagggga atcgccagca acaccactaa atcaaccgct tccgggccat ccacgccttc
    14221 ccactgaagc ggctcgctga gtgtggcgac cgcaaacgcc gcttctttta ccgcagcagt
    14281 tttgccatgc ggcacagcca acccttcact taaggcggtc gggccaaggc tttcacggcg
    14341 atacacttct ttcagaaatt gctcagtact ggaaattttc cccagagcag caagacgttg
    14401 agtcaacgcg tgaatggcct cttcacggct ggtaaagcgc gcattcagac acaacgcatc
    14461 gcggtgggtc agagtcgtca ggttcatcat gatcctcact tgatcgtttt tatagtcgcg
    14521 ccagtgtgcc cgacaaaaca ataccattgc gtaatcaatt tcacttgatt gaaattattt
    14581 gcattttatt tgtattgttt acaggatgag tttatcgggc ataatggcta aattcgtatt
    14641 aatgagatgc taaataatgg gacacaaacc cttataccga cagattgctg atcgcattcg
    14701 tgagcaaatt gcgcgtggcg agcttaaacc tggcgatgcg ttacctaccg aatcggcatt
    14761 acaaacagag tttggcgtca gccgggttac ggtgcgtcag gctttgcgcc agttagtcga
    14821 gcaacagatc ctcgaaagca ttcagggcag cgggacttac gtcaaagaag agcgggtcaa
    14881 ttacgatatt tttcagttaa ccagttttga tgaaaaactg tcggaccgtc acgtcgatac
    14941 acacagtgaa gttctgatat ttgaagtgat tccggctgac gattttcttc agcaacagct
    15001 acaaatcacc gtgcaggatc gtgtttggca tgtgaagcgc gtgcgctatc gcaagcaaaa
    15061 gccaatggcg ctggaagaaa cctggatgcc gcttgcgttg ttcccggatc tcacctggca
    15121 ggtcatggaa aattcgaaat atcactttat tgaagaagtg aagaagatgg ttatcgatcg
    15181 tagcgaacag gaaatcattc ctctgatgcc aaccgaagag atgagccgtc tgcttaatat
    15241 cagccagaca aagccgattc ttgaaaaagt atcgcgtgga tatttagttg atggtcgggt
    15301 gtttgaatat agccgcaacg cttttaatac cgatgactat aaatttaccc tgatagcgca
    15361 gcgaaaatca tcgcgataag cacaaaaagg ccatcatacg atggccaacc atgtcgaaac
    15421 cgacagccct tcgccattcc tgtagaggga agcggcgagg gctatttctt attacagata
    15481 tttatttcag aacagttttc agtgcttcac cgatatccgc caggctgcga acggttttca
    15541 cgcctgcggc ttccagagca gcgaatttct catccgcagt ccctttccca ccggcaatga
    15601 tcgcacccgc gtggcccata cgtttgcctt tcggcgcagt cacaccagcg atgtaaccca
    15661 caactggctt ggtaacatgc tctttgatgt acgctgccgc ttcttcttca gcgctaccgc
    15721 cgatctcacc gatcatcacg atcgcttcgg tctgcggatc tttttcgaac atttcgagaa
    15781 tgtcgataaa gttagagcca gggatcgggt caccgccgat accgacacag gtcgactgac
    15841 cgaaaccgta atccgtggtc tgtttaaccg cttcataggt cagtgtaccg gaacgggaaa
    15901 cgatacccac tttacccggt ttgtgaatgt gacccggctg gataccaatt ttgcattcac
    15961 ccggggtgat aacgcctggg cagttcgggc cgatcatacg aacgcctgct tcgtccagtt
    16021 tcactttcac ggtcagcata tccagcgtcg ggatgccttc agtgatggtg ataatcagtt
    16081 tgatgcctgc atcgatagct tccagaatgg agtctttgca gaacggtgct ggtacgtaaa
    16141 taacagacgc ggaagcgcca gtagcagcaa cagcttcacg cacggtgttg aacaccggca
    16201 ggccgaggtg ggtagtgccg cctttacctg gagttacgcc accaaccatt ttagtgccgt
    16261 atgcaatggc ctgttctgag tggaaagtcc cctggctacc ggtaaagccc tggcagataa
    16321 ccttggtgtt tttatcaatt aaaatggaca ttatttcccc tccactgcgg caacaacctg
    16381 ctgagctgca tccgtcagac cttttgctgc aataatattc aggccgctgt cagccagttt
    16441 cttcgcgccg agttcggcgt tgttaccttc cagacgtacc acgaccggta cgttaacacc
    16501 cacttccgct accgcgccga tgataccgtc agcgatcagg tcgcaacgaa cgataccgcc
    16561 gaagatgtta accagaacgg ctttcacttt gtcgtcagag aggatgattt tgaacgcttc
    16621 ggttacacgt tctttggttg cgccgccgcc aacgtccagg aagttagccg gttcgccgcc
    16681 gtgcagttta actatgtcca tcgtacccat cgccagacct gcgccgttaa ccatacaacc
    16741 gatgttaccg tccagcgcaa cgtagttcag ttcccactgt gcagcctgtg cttcacgcgg
    16801 atcttcctgc gactggtcac gcatttcgcg cagatcaggc tggcggaaca gggcgttgcc
    16861 atcagcgccc agtttgccgt cgaggcaaat cagatcgccc tgtttggtga tgaccagcgg
    16921 gttgatttcg atcagcgcca ggtcgcgctc caggaaaatg gtcgccaggc ccatgaagat
    16981 tttggtgaac tgctgaacca gtttaccttc cagacccagt ttgaacgcca gctcgcgtcc
    17041 ctgatacggc atcgggccag tcagcggatc aagcgcaact ttatggatca ggtgcggagt
    17101 ttcttccgcc actttttcga tttccacgcc gccttcggtg gaggccataa agaccacgcg
    17161 acgggaacta cggtctacaa cggcaccgag atacagctct ttagcgatat cggtcgccgc
    17221 ttcaaccaga atctggttaa ccggttggcc attggcatct gtttgatacg ttaccagacg
    17281 cttgcccagc cagttttctg caaaagcacg gatgtcttct ttgctgttta caactttcac
    17341 accgcccgct ttaccgcggc caccagcgtg aacctgacat ttcactaccc acggaccggc
    17401 accgattttt gaagcggctt cttctgcttc gcgcggagta gtacaggcat aacccaccgg
    17461 tgctggtaag ccatagcggg caaacagttg ttttgcctga tattcatgta agttcatgtg
    17521 ttctgtccat ccttcagtaa tcgttatctt ttaaaccgta gaccgacacc ctatccacgt
    17581 cggtctacag tgcaggtgaa gcttaaacta ctacacgtcc agcagcagac gcgtcggctc
    17641 ttccagtaac tctttgatcg ttaccaggaa gcccacggat tcgcgaccat cgatcagacg
    17701 gtgatcgtag gacagcgcca ggtacatcat cggcaggatc tcaacctgac cattcaccgc
    17761 catcggacga tctttgatag cgtgcatacc cagaattgcg ctctgcggcg ggttgatgat
    17821 cggcgtagac atcagggaac cgaacacacc accgttggtg atggtgaagt taccaccggt
    17881 cagatcttca accgtcagct tgccatcacg gcctttgact gccagctctt tgattttctt
    17941 ctcgatgtct gccatgccga gggtatcgac atcacgcaga accggcgtca ccaggccgcg
    18001 cggcgtagaa accgccatgc tgacgtcgaa atagttgtgg taaaccacgt catcgccgtc
    18061 gatagacgcg ttcacttccg ggtaacgttt cagggcttca accaccgctt tcacgtagaa
    18121 ggacataaag cccagacgga tgccgtggcg tttttcaaac gcttcaccgt actgcttacg
    18181 cagatccata atcggcttca tgttgacttc gttgaacgtg gtcagcatgg cggtggagtt
    18241 tttcgcttcc agcagacgct ctgccacacg cttacgcagg cgagtcatcg ggacacgttt
    18301 ttcactacgt gcagccagag ccggttgcgc cgccggagca gccgctgccg gagcagactc
    18361 tttcgccggg gctttcgcca gatgtttttc cacatcttca cgagtcagac gaccacccac
    18421 accggtgcct ttaatggcgc tggcgtcgag attgtgttca gccagcaggc gacggatcgc
    18481 cgggcttaac gcatcgttgt tttgctcttc cagagacgcc tgctggcgtt gcgccggagt
    18541 ggacgctttc tcttcagatt tggcgctggt ttctttaccg gcgctgttgc cttcacgcag
    18601 gcgaccaagg atctgacgag acgttaccgt tgtaccttca tcttccagaa ccgcatccag
    18661 aatgccgtct gctgatgccg gtacttccag taccactttg tcagtttcga tttctaccag
    18721 cacttcatca cgtacgactg cgtcgccggg ttttttatgc caggttgcga cggtggcatc
    18781 ggctacggat tcaggcaggt cagggaccag aatatctacg ctactcattg tgtatccttt
    18841 atttattcga cgttcagcgc gtcattaacc agatcttgtt gctgtttctg gtgaacggac
    18901 atatacccta ccgccggaga ggcggaggcc gggcggcctg cataacgcag agaagcccca
    18961 aacggaatca cttcacggaa atgatgctgg ctgcagtacc atgcgccctg gttgagcggc
    19021 tcttcctggc accagacaaa atccttgacg tgagcaaact gctgcaacac ttcctgcatc
    19081 gctttatgcg ggaacgggta gagttgctcg atacgcacaa tggcgacatc gtgttgattg
    19141 ttcttacgac gctgttccag caggtcgtaa taaaccttac cagaacacat cactacgcgc
    19201 ttcacgccct tcggatcaag ctcgtcgatt tcaccgatgg ctggcaggaa ggtgccgttc
    19261 gccagttctt cgaggctgga aaccgccagc ggatgacgca gcagggattt cggcgacatc
    19321 acgaccagcg gacgacgcat cccgcgcagc gcctgacgac gcagcatgtg gtaaacctgt
    19381 gccggggtag acggtacgca aacctgcatg ttttgctcag cacaaagttg cagataacgt
    19441 tccagacgcg cggaggagtg ctccggcccc tgcccttcgt aaccgtgcgg cagcaacatc
    19501 accagaccac acatccggcc ccatttctgc tcgccagagg agatgaactg gtcgataacc
    19561 acctgcgcac cgttggcgaa gtcaccgaac tgcgcttccc agatggtcag ggtgcgtggt
    19621 tctgcggtgg cataaccata ttcaaacgcc agcacggctt cttctgacag tacggagtcc
    19681 cagacacgga acgcgccctg accgttatgg atgtgttgca gcggcgtgta agtggaaccg
    19741 ttagactggt tgtggatcac cgcgtggcgg tggaagaagg taccgcgacc ggagtcttca
    19801 cccgacaggc gaaccggaat gccttcatca accagcgtgg cgtaagcgag gttttccgca
    19861 ccgccccagt cgaacagttt ctcaccggct gccatcgcct ggcgatcgcc ataaatcttg
    19921 gcaacgcgag actgcatttc aactgcttct ggtaccgtac tgatacgttt agccagttcc
    19981 tgcaggcgct tcatctcaac tttgttcggg tactcttcgt cccattcgtg gttgaggtac
    20041 ggcgaccagg tgaaagagtg catgttcatc ggacgccact ctgccactac gcaatcgcca
    20101 gcatccagcg catcgcggta caggttaacc atctcggtgg catcttccag cgtcgccact
    20161 ttttcctgct ccagcttgtc agcgtagatt ttgcgcggtg tcggatgttt tttgattttc
    20221 tgatacatca gtggctgggt tgcgctcggc tcgtcggctt cgttgtggcc gtgacggcgg
    20281 tagcatacca ggtcgatgaa gacatcacgt ttaaaggtgt tacggaaatc gagcgccaga
    20341 cgggtcacaa aggcaacggc ttccggatca tccgcgttaa cgtggaaaat cggtgcctga
    20401 accatcttac cgatatcagt acagtacggt gtagaacggg catccagcgg gttagaggtg
    20461 gtgaaaccaa cctggttgtt gataacgata cgtaccgtac cgccaacttc ataaccacgc
    20521 gctttcgaca tgttcagagt ttcctgaacc acgccctgcc cggtcactgc ggcgtcaccg
    20581 tggatggtga ttggcagcac tttgttgctg ttcggctcat caagtctgtc cagacgggca
    20641 cgaacagagc cgataacgac cgggcttaca atctcaaggt gagacgggtt aaacgccagc
    20701 gccaggtgca ccaggccgcc atcggtctgg aagtcagacg agaagcccat gtggtatttc
    20761 acgtcgcctg tgccgaggtg ttctttatgt ttaccggcga actcgtcgaa caagtcttgc
    20821 ggttttttac ccagcacgtt caccagcacg ttcagacgac cacggtgcgc catcccgaga
    20881 accacttcgc gggtgccgct gttgccagcg tggcggatca tctctttaag catcgggatt
    20941 aacgcgtcac cgccttccag cgagaagcgt tttgcgccag ggaattttgc gccgaggtaa
    21001 cgctcaaggc cttcagcggc ggtcagttcg cttaagaagc gttttttctc ttcgctattg
    21061 aaagtcgcgc gaccagactc gatacgctgt tggatccagc gtttttcttc ggtgctggtg
    21121 atgtgcatat actcggcacc aatcgggccg cagtaggttt gcttgagggc ggaaataagc
    21181 tcgccgagtt tcatggtttc tttgccgctg gcaaatgaac cgacgttgaa ggtttcctgg
    21241 aagtctgctt cggtcagatc gtggaaagac ggatccagat cggccacttt atcttgctgc
    21301 cacagtccca gcggatctag attcgcatgc tggtgaccac ggaagcggta tgcgttaatg
    21361 agctgcagga ctttaacctg cttcacattg gtgtcagggt cggagatcgt tgaagagtaa
    21421 cgtgaagcgt ctttcgccag gcggcggaaa tattcacgcg tttgagagtg gaattgatcc
    21481 ggtttgactc ccgtaccagg taactgctgg aacgtcgaac gccagttagc gtcaaccgag
    21541 tcaggatcgg ttaagaagtc ttcatagagc tgttctatcc agctctggtt tgcgccagag
    21601 aggtaagaag agtccaacca ggctttcaaa gcgctgttct gcatcgtgat cccttaagca
    21661 tcttttttat gcttacttcg ccgtggatac taccacgcac agtgatgtgc gtgccggggt
    21721 tcacttgcga gctcttgtct acgtagtggc tcgcgaagga acctttaaaa actgtcttag
    21781 tgtcaggggc agtttttaaa gatttcctgc atttctttgt tacgcctgat gcgctgcgct
    21841 tatcaggcct gcgggtaatg acttgtaggc cggataaggc gttcacgccg catccggcac
    21901 tggttgcctg atgcaacgct tgcgcgtctt atcaggccta cagtttacgc attacgttgc
    21961 aacaacatcg acttgatatg gccgatggcg cgcgtcgggt tcagcccctt cggacataca
    22021 ctgacgcagt tcatgatgct gtgacagcgg aatacgctga atgcatcgct caaaccgtcg
    22081 aggcggctgt cagtctcggt atcacggcta tcgatcagga aacgatatgc cgctaacaag
    22141 cctgccgggc cgataaactt atcgggattc caccagaaag acggacaaga ggttgaacaa
    22201 catgcgcaga gaatacattc atacagcccg tcgagttttt cgcgctgctc tggcatctgt
    22261 aaatgctcgc gagctggcgg attttgtcca ttattcaaca ggtaaggctt aattttctca
    22321 tattgcgcat agaattgtcc catgtctacc accaaatcgc ggatcaccgg taaacctggc
    22381 agcgggcgaa tcacaatctt cttgcccggc tggttgagtg ccgaaatcgg ggtaatacag
    22441 gccagaccat tcttgccgtt catgttcaga ccgtcggaac cgcacacacc ttcacggcag
    22501 gagcggcgga acgacaggct gggatctttc tctttcagct ggataagcgc atccagcagc
    22561 atcatgtcgc gaccttcttc cgcttccagg gtgtaatcct gcatacgcgg agcatcatca
    22621 acatccgggt tatagcgata aattgaaaac tcgagtctca ttttcctgtc tccgcattag
    22681 taagtacgaa tcttcggcgg gaatgccggg cgcagtttcg gttccatgtt gacgcttcgg
    22741 cgcgtcatgg attccgactc aggcagatac agggagtggc acagccagtt ttcatcatca
    22801 cgatccggga agtcgaagcg gctatgtgcg ccacggcttt cggtacggaa gttggcagaa
    22861 acagccgttg catacgccgt ttccatcagg ttatccagtt ccaggcactc aacgcgctgg
    22921 gtgttgaact cgctggaagt gtcatccaga cgggcatttt tcagacgctc gcggatcact
    22981 ttcaactgct caagcccttt cgccatcgca tcaccttcac ggaagaccga gaagttatgc
    23041 tgcatacatt cttgcagcgc tttacggatc gccaccggat cttcaccgtt acgattattg
    23101 ttccaccggt tcaggcgatc cagagacgct tcaacatcag actcgctggc atcgtgcagt
    23161 gcgccctgct cggcgataga ctcttgcaga tgcagacctg ccgcgcgacc aaagaccacc
    23221 aggtccagca gcgagttgcc gcccagacgg ttagcgccgt gtaccgatac acaagcgatt
    23281 tcaccaacgg caaacagtcc cggaacaacc acatcttcgc ctttctcatt cacagtcagc
    23341 gcctgaccgg taactttggt cggaataccg cccatcatgt agtgacaggt tgggataacc
    23401 ggaatcggct ctttcaccgg atcgacgtga gcgaaggtac gggaaagctc caggataccc
    23461 ggcagacggg attcgagaac ttctttaccc aggtgatcga gtttcagttt cgcgtgtggc
    23521 ccccacggac catcacagcc gcgaccttca cggatttcaa tcatgatgga acgcgccacc
    23581 acatcacggc ccgccaggtc tttggcgttc ggcgcataac gctccataaa gcgttcgcca
    23641 tgtttgttca gcagataacc gccttcacca cggcaacctt cggtgaccag tacgcccgca
    23701 ccggcaatgc cggtcgggtg gaattgccac atttccatat cctgcaccgg tacgcctgcg
    23761 cggatagcca tgccgacacc gtcgccagtg ttaatgtggg cgttagtggt ggactgataa
    23821 atacgccctg ctccgccagt cgccagcacg gtagcgcggg ctttgaaata aaccacttca
    23881 ccggtttcga tgcacagtgc ggtacaaccc accaccgcgc catcctggtt tttcaccaga
    23941 tccagcgcat accactcgga gaaaatggtg gtgtggtttt tcaggttctg ctgataaagc
    24001 gtgtgcaaca gtgcgtgacc ggtacggtca gcagccgccg cagtgcgtgc cgcctgctcg
    24061 ccgccgaagt ttttcgactg accgccaaac ggacgttgat agatacgacc atcatcgaga
    24121 cgcgagaacg gcaggcccat gtgttcgagt tccagaatcg cttccggccc ggttttacac
    24181 atatattcaa tcgcgtcctg gtcaccgata tagtccgacc cttttacggt gtcgtacata
    24241 tgccattccc agttatcttc atgggtatta cccagcgcaa cggtaatgcc gccttgcgca
    24301 gaaacggtat gggaacgggt cgggaagact ttagagagca gggcacaggt ctggccgctc
    24361 tgggaaattt gcagcgccgc gcgcatacct gcgccaccgg caccaatcac aactgcatca
    24421 aattctctga ctggcaattt catcacacac cccacaccac aacgaatcca taaatcacgt
    24481 aaaccaccag tgcaacgaca atcaccagtt gcagcatcag gcgcaaggcc agcggtttaa
    24541 cgtagtcggt caacacctgc cacatgccga tccaggcatg aatcaagata gaaaacagcg
    24601 ccagcagggt gaacactttg gtgaacgcag aggcgaagaa accgatccag acttcatatg
    24661 tcagttcgcc actggtggcg aaaaaaccga ccatataaat gatgtagagc gtcaggacga
    24721 tagcggtagc gcgaacgagg atgaaatcat gtacgccatt gcgtcctaat gcggaggcgt
    24781 tgcttaccat acgaggactc ctgcgagaag tgaaagcacg acagtaataa caaaggagat
    24841 tttggcggag cgtttacccg cttcgaatgt ttcttccaga tagccaaaat ccatcatcat
    24901 gtggcgaata cctacgacga cgtgatacgc cagagcggta aggatgcccc acatgataaa
    24961 tttgacgaag aagctgccca taatcgcgga agcttgctcg aaaccttcag gggaagagag
    25021 gctggtaccc agaagccact gcaggatgcc cactgcaaca aaggtgatca caccggaaac
    25081 gcgatggaga atggacgcta tcgccgtgat ggggaaccgg atggtctgta ggtccagatt
    25141 aacaggtctt tgttttttca catttcttat catgaataac gcccacatgc tgttcttatt
    25201 attccctggg gactacgggc acagaggtta actttctgtt acctggagac gtcgggattt
    25261 ccttcctccg gtctgcttgc gggtcagaca gcgtcctttc cataactgcg cgtcatgcaa
    25321 aacactgctt ccagatgcga aaacgacacg ttacaacgct gggtggctcg ggattgcagg
    25381 gtgttccgga gacctggcgg cagtataggc tgttcacaaa atcattacaa ttaacctaca
    25441 tatagtttgt cgggttttat cctgaacagt gatccaggtc acgataacaa catttattta
    25501 atttttaatc atctaatttg acaatcattc aacaaagttg ttacaaacat taccaggaaa
    25561 agcatataat gcgtaaaagt tatgaagtcg gtatttcacc taagattaac ttatgtaaca
    25621 gtgtggaagt attgaccaat tcattcggga cagttattag tggtagacaa gtttaataat
    25681 tcggattgct aagtacttga ttcgccattt attcgtcatc aatggattct ttacctgcaa
    25741 gcgcccagag ctctgtaccc aggttttccc ctctttcaca gagcggcgag ccaaataaca
    25801 aacgggtaaa gccaggttga tgtgcgaagg caaatttaag ttccggcagt cttacgcaat
    25861 aaggcgctaa ggagacctta aatggctgat acaaaagcaa aactcaacct caacggggac
    25921 acagctgttg aactggatgt gctgaaaggc acgctgggtc aagatgttat tgatatccgt
    25981 actctcggtt caaaaggtgt gttcaccttt gacccaggct tcacttcaac cgcatcctgc
    26041 gaatctaaaa ttacttttat tgatggtgat gaaggtattt tgctgcaccg cggtttcccg
    26101 atcgatcagc tggcgaccga ttctaactac ctagaagttt gttacatcct gctgaatggt
    26161 gaaaaaccga ctcaggaaca gtatgacgaa tttaaaacta cggtgacccg tcataccatg
    26221 atccacgagc agattacccg tctgttccac gctttccgtc gcgactcaca tccaatggca
    26281 gtcatgtgtg gtattaccgg cgcgctggcg gcgttctatc acgactcgct ggatgttaac
    26341 aatcctcgtc atcgtgaaat tgccgcgttc cgcctgctgt cgaaaatgcc gaccatggcc
    26401 gcgatgtgtt acaagtattc cattggtcag ccatttgttt atccgcgcaa cgatctctcc
    26461 tatgccggta acttcctgaa tatgatgttc tccacgccgt gcgaaccgta tgaagttaat
    26521 ccgattctgg aacgtgctat ggaccgtatt ctgatcctgc acgctgacca tgaacagaac
    26581 gcctctacct ccaccgtgcg taccgctggc tcttcggggg cgaacccgtt tgcctgtatc
    26641 gcagcaggta ttgcttcact gtggggacct gcgcacggtg gtgctaacga agcggcgctg
    26701 aaaatgctgg aagaaattag ctccgttaaa cacattccgg aatttgttcg tcgtgcgaaa
    26761 gataaaaatg attctttccg cctgatgggc ttcggtcacc gcgtgtacaa aaattacgac
    26821 ccgcgcgcca ccgtaatgcg tgaaacctgc catgaagttc tgaaagagct gggcaccaaa
    26881 gatgacctgc tggaagtggc tatggagctg gaaaacatcg cgctgaacga cccgtacttt
    26941 atcgagaaga aactgtaccc gaacgtcgat ttctactctg gtatcatcct gaaagcgatg
    27001 ggtattccgt cttccatgtt caccgtcatt ttcgcaatgg cacgtaccgt tggctggatc
    27061 gcccactgga gcgaaatgca cagttacggt atgaagattg cccgtccgcg tcagctgtat
    27121 acaggatatg aaaaacgcga ctttaaaagc gatatcaagc gttaatggtt gattgctaag
    27181 ttgtaaatat tttaacccgc cgttcaaatg gcgggttaat ttttatatgc ctaaacacaa
    27241 aaaattgtaa aaataaaatc cactatcgga cattcatata ttctaaaaaa aaatacatcg
    27301 gtataaatta taaacaaata gatgattttt aatcatctat cttcctctca gttacatgaa
    27361 taaaccacca aatatagcgc acccctataa ttcccaatac tttattcatc aaacatttaa
    27421 ttcataccat caattaatta cttttacatt taataaaatt aataatattt ttaagaaaaa
    27481 ctaaaaacat tcgacaatca aataagatat tcttgcaccg atatactttt ttcttttctg
    27541 ttaaaggact cagttatgtt taaaggacaa aaaacattgg ccgcactggc cgtatctctg
    27601 ctgttcacag cacctgttta tgcggctgat gaaggatccg gtgaaattca ctttaaaggt
    27661 gaagttattg aagcaccgtg tgaaatacat caggatgata ttgataaaga ggttgaactc
    27721 ggtcaggtga ccaccagcca cattaatcag tcacatcaca gcgatgccgt tgctgtcgac
    27781 ctgctcttag tcaactgtga tctggaaaac tccagcaacg gttccggtgg caagatttct
    27841 aaagttgcag tcacctttga tagctcagcg aaaaccaccg gcgcagatcc gattctcaac
    27901 aacaccagca cgggtgaagc caccggcgtc ggcgtacgtt taatgaataa agatcaaagc
    27961 aatatcgttt taggcaccgc tactccagat atcgacctgg ctccgacctc cagcgaacaa
    28021 acgctgaatt tctttgcctg gatggaacaa attgatcagg ctacacctgt aacgccaggc
    28081 gcagttacgg cgaatgcaac ctacgtgctg gattataaat aagtattact ttggcggcgc
    28141 tatcgccgcc tttcataaaa tatttatcag gtatggacac cgtgaatatt tatcgactct
    28201 cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt ggcggtcgaa ttcaatctga
    28261 atgttctcga taaatcaatg cgcgaccgca ttgatatttc attattaaag gaaaaaggag
    28321 tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa taataaccaa atcagtaacg
    28381 agcaaaagat taactggcac aaaaatgacg ataaaaccat tccgtgcatc aatgatttac
    28441 tggtcgataa atttggctta aaacctgaag tccgtcagtc gttaccattg ataaatcagt
    28501 gcgtcgattt tagctcccga cctgaaatgc tcttcaattt cgatcaagcc aatcagcaac
    28561 tgaatattag tattccgcaa gcctggctgg cgtggcactc agaaaactgg accccaccct
    28621 ccacatggaa agaaggtgtc gccggtgtcc tgatggatta caacttgttt gccagcagct
    28681 accgcccaca ggatggcagc agcagcacta atcttaacgc ctatggaaca gccggaatca
    28741 acacgggggc atggcgctta cgcagtgatt atcagttgaa tcatactgat agcgatgata
    28801 accatgaaca gtcaggcgaa atatcacgta cctatctttt tcgcccgtta ccgcaattag
    28861 gttcaaaatt aaccctcggc gaaacggatt ttagtcccaa tattttcgac ggtttttctt
    28921 ataccggcgc ggcactggca agtgacgatc gaatgttgcc atgggaacta cgcggctacg
    28981 ccccacaaat tagcggtatt gcacagacca atgccacggt gacgatcagt caatcaggcc
    29041 gcgtcattta ccagaaaaaa gtcccaccag gcccatttat cattgacgac cttaatcagt
    29101 ctgttcaggg cacactggat gtcaaagtga cggaagaaga tggtcgggtg aacaatttcc
    29161 aggtttcagc agcatcgacg cccttcctga ctcgtcaggg acaggttcgc tataaactgg
    29221 ccgcgggtca gccacggccc tccatgtcac atcaaactga aaatgaaacc ttttttagca
    29281 atgaagtttc ctgggggatg ctgtcaaaca cctcgctgta cagcggcctg ctgctttctg
    29341 gtgatgacta ccattctgcc gcaatgggta ttgggcaaaa tatgctgtgg cttggtgcgc
    29401 tgtcgtttga tgtcacgtgg gccagtagcc attttgatac tcagcaggac gagcggggct
    29461 taagctaccg ttttaattac agcaaacaag tggatgccac taacagcacg atttccctcg
    29521 ccgcttatcg tttctccgat cgtcattttc acagctacgc caactatctg gatcacaaat
    29581 acaacgacag cgatgcgcag gacgaaaaac agacgatcag cttatctgtg ggccaaccga
    29641 ttaccccact aaacctcaat ctttacgcca acctgctaca tcaaacctgg tggaatgcag
    29701 acgcctccac gaccgccaac atcacggcag gttttaatgt tgatattggt gactggagag
    29761 atatctctat ttctacgtca ttcaatacaa cccattacga agataaagat cgcgacaacc
    29821 agatttacct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc tatgacatgc
    29881 aaaacagtag ccacagcacc acacaccgca tgtcgtggaa cgatacgctg gatgaacgta
    29941 atagctgggg catgtctgcc ggactgcaat ccgaccgtcc tgacaatgga gcccaggtga
    30001 gcggtaacta tcagcacctg agttcagcgg gtgagtggga tatttctggt acctatgccg
    30061 ccaatgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca acccaatatg
    30121 gtgcagcgtt tcatcgccgc agctccacca atgaaccacg cctgatggtc agcaccgatg
    30181 gcgtggcaga tattccggtt cagggcaatc tcgactacac caaccatttt ggcattgcgg
    30241 tggtgccgtt gatttccagt taccagcctt ccaccgtggc ggtgaacatg aatgacttac
    30301 ccgacggcgt aacagttgca gaaaacgtta tcaaagaaac gtggattgaa ggcgcgatag
    30361 gttacaaatc actggcttcc cgttccggta aagacgttaa cgtcatcatt cgcaacgcca
    30421 gcggtcagtt tcctcccctc ggagcggata tccgccagga tgacagcggc attagcgtgg
    30481 ggatggttgg cgaggaagga catgcctggt taagcggagt cgctgaaaat caaaagttta
    30541 ccgtggtctg gggtgatagc cagcattgct cgctccatct tcctgaacat atggaagaca
    30601 ccgcaaatcg cctgatttta ccttgtcatt aattaaggaa atgaaaatga catttatgaa
    30661 aggactaccc cttttgttgt tggttgccag tctgtgcagc cacgctgcac tacaacccga
    30721 tcgcactcgt attgtgttta acgctaatga taaagctacc agtctgcggg tggataatcg
    30781 tagcgataaa ctcccctacc tcgcttattc ctggctcgaa aatgaaaaag gggagaaaag
    30841 cgatgatctt ctggtcgccc tgccgccgat acaacgtctg gaaccgaaag caacaacaca
    30901 ggtacggata gtaaagcaag cctcgaccac aaaattaccg ggcgatcgcg aaacgctatt
    30961 tttctacaat atgcgtgaaa ttcctcccgc cccggaaaaa aacagcgacc atgccgttct
    31021 gcaagtcgcc attcagagtc gtatcaaggt gttctggcga ccggctgcat tacgcaagaa
    31081 agcaggagaa aaggtcgaac tgcagttaca ggtcagccag cagggcaacc aactgacact
    31141 gaaaaatcct accgcgtatt atctgaccat tgcctacctg ggacgaaatg aaaaaggcgt
    31201 tctccccggc ttcaaaaccg tgatggttgc gccttttagt acggtcaaca caaacaccgg
    31261 aaattatagc ggcagccagt tttatctcgg ttatatggat gattacggcg cgttacgcat
    31321 gacgacgctc aactgcagcg gacaatgcca tttacaagcc gtggaggcga agaaatgagt
    31381 gctggcaagg gagtgttgct cgtcatctgt ctgttatttc tgtcgttgaa gtctgcgctt
    31441 gccctgaact gctactttgg ttcatccggt ggctcggtag aaaaatcaga agcgattcaa
    31501 ccttttgctg taccaggcaa tgccaaactc ggcgataaga tctgggaatc tgacgatatt
    31561 aaaataagat ctgggaatct gacgatatta aaattcccgt ctattgtgac aacaatacca
    31621 acggcaactt tgaaagcgaa catgtctacg tatgggtaaa cccctacccg ggggggcagg
    31681 atcgttatta tcaactcgga gtaacttaca atggcgttga ttatgacgcc aaccagggaa
    31741 aaagccgcat cgacactaac cagtgtatcg acagtaagaa cattgacatt tatacccctg
    31801 atgggatggc agaacaaaat ttgctcgggt gatcccgcta atattcacat gtcgcgaacc
    31861 tttctcgctc gcatgcggtt atacgtcaaa atacgagaaa tgccgccgca tgattatcaa
    31921 agtacgctta gcgactatat cgtcgtgcaa tttgacggtg ccggtagcgt taacgaagac
    31981 cctactgccc aaaacctgaa atatcatatt actggtctgg aaaacattcg cgtgctggat
    32041 tgcagcgtca atttttccat ttcaccggaa acacagatga ttgattttgg taaatttaat
    32101 ttgctggata ttcgtcgcca cacaatgtcg aaaacattca gtattaagac gacaaaatca
    32161 caaaatgacc aatgcaccga cggatttaaa gtcagttcct ccttttatac cgaagaaacg
    32221 ttggttgaag aagataaagc attgttgatt ggtaatggtc tgaagctacg tttgctggat
    32281 gaaaacgcct cgccttatac cttcaataaa tatgctgaat acgccgattt caccagcgac
    32341 atgttggtct acgaaaaaac ctatacagct gaactttcgt ccattgcagg tacccccatc
    32401 gaggctgggc ccttcgatac agtggtgctt tttaaaatta actataactg atttgccatg
    32461 atgccctttg cagccggaaa gggcatctct ttcagaaaat tgctgatttt ccccttttcc
    32521 ttgattgctg ttattttttc ctagcccggc tttctttatg ctgtatcgat acaccaaaga
    32581 gaaaataatg agggagcgaa ggatgccagt tttgcagtgg ggaatgttat gtgtgctatc
    32641 acttctcctt tctattggtt tcctcgcggt acatctcccg gcggcgctat tacttgggcc
    32701 gatgatcgcc gggatcatct tcagtatgcg cggaattact ctgcaacttc cccgctccgc
    32761 ttttctcgcc gcgcaggcca ttcttggctg catgattgcg caaaacctca ctggttcgat
    32821 tctcaccacg ctggccgcca actggccgat cgtgcttgct attttactgg tgacgctgct
    32881 ttccagcgcc atcgtgggtt ggttattggt gcgctatagc tcactgcctg gaaataccgg
    32941 tgcctggggt tcttctccag gcggcgcagc ggcaatggtc gctatggcgc aagattacgg
    33001 cgcagatatt cgcctggtgg cgtttatgca atatctgcga gtgctgttcg tcgcgggggc
    33061 cgctgttctg gtcacccgca tgatgctggg tgataacgct gaagcggtta accagcaaat
    33121 cgtctggttc ccgccagtga gcattaatct cctgcttacg attttgctcg cagtcgttgc
    33181 cggtacggcg ggatgcatgc tgcgtcttcc ttccggcacg atgctcatcc cgatgctggc
    33241 gggcgcagtg ctccagtctg gtcagctcat caccatcgaa ctaccggaat ggctgctggc
    33301 gatggcgtat atggcaattg gctggcggat tggtcttggt ttcgataaac aaatcttact
    33361 gcgggcacta cgcccgctac cgcaaatcct gctgtcgatt tttgctctgc tggctatttg
    33421 tgcaggtatg gcgtggggac tgacccggtt tatgcatatt gattttatga ctgcctacct
    33481 cgccaccagt cccggcgggc ttgataccgt ggcggtcatc gccgcaggga gcaatgccga
    33541 tatggcgctt attatggcga tgcaaaccct gcgcctgttc agtattttgc tgacggggcc
    33601 agccattgcg cggtttattt caacctatgc gccgaagcgg tcggcctagt gctggcagcc
    33661 tggacaccag taaaacgggc gcgaggacaa cgtggttttc tcaatgatgc cgccacagcg
    33721 ttcgcacagt tcgccatcac gatgaaaaac cttaaagcga aacagcgccc catgatgctt
    33781 attctcatcc acctgccccc gcgtagcgta agaaaaccga ggaatctcca gtaacgcgtg
    33841 tgcgagtgca tccagttgcg ccgcattgag atctttcgct ttatgatttc cagtcaaccc
    33901 aacctgccag aggatctcca cccgcaaata attgccaagc ccagccagaa acgcctgatc
    33961 gagcagtaat cctgcaaact gacggttacg aaagcgcggc gacaataacc gttcctttac
    34021 cacctccggc gtcagattcg gatccagcac atcgggacca acgcgttgta aaaacggatg
    34081 cgtggtcagt tgttccggcc tcaacatctc aatatccgag gcgctataaa gcagaatggt
    34141 tttgtcagcc gtttgcagtt ttacccgcaa tactcgcgtg gtctgcggct cttcgccggt
    34201 atcaaccacg cgccagacgc cgtaaagctg attatggctg tagagcgtta aaccgttggg
    34261 aaaatgagtt aacaacgcct taccacgcgt ttccacatgg gtaacgtgtt gaccgataag
    34321 ttgtgattga taagttttta actgcgggaa ggcaaaccag acatcagtta gtggtttgcc
    34381 tttgatcgcc gcctccaggt tatccgctgc acggcggatc tccgggcctt caggcatggt
    34441 gatatccttt tttcattctt tattaagcgg ggttatgctg cgacaacaat cccctttttg
    34501 gcaaatgtag ctcgtaatcg gcgggcgaac gccagagcgt gctcgccgtc gccatgcagg
    34561 cagaccgttt gcgccgcgac cgttgcccat tcgccggtga tacttttgac tctgccgtgt
    34621 tgcaccatct ccagcgtttg cgccaacgcc tgttcttcgt tttcaatcaa cgcgcctggc
    34681 tgacttcgcg gcaccagcga gccgtcagcc tgataaccgc gatcggcaaa cacttcctcg
    34741 cgcgttgtca ggccatattg cttgcctgca cgaatcagct cgcttcctgc cagcccaacg
    34801 agaaccaatg ccggatcgca agcgtatacc gctctggcga tggcgtctgc cagttgtgct
    34861 tctttcgccg cctggttgta caacatgccg tgcggtttga catgacgcat tacgccgcct
    34921 tgcgcacggg caatcgttgc cagcgcgcca atttgataca gcgtctgggc gtaaacggtt
    34981 tccggcggca gctgcatggc gctgcgacca aaattttccc tgtcgggaaa actcgggtga
    35041 gcgccaatcg cgacaccatt ttttattgct tcacgcacgc aagcctgcat ggtttgcgca
    35101 tcgcctgcat gaaatccaca agcaatattg gcagaggaaa ccagcgttaa tagctctgcg
    35161 tcgctggcgc agccttcgcc cagatcggcg ttcaggtcaa ttttcattgt gcagccgcca
    35221 cgctaattgt tcaaaataac gttgctgatc ttgccgcgct ttcagtgcct cttccagtga
    35281 acactggaca aaatgaatcg gctgaccgag cggaatttgc gccagatggt acatatcagc
    35341 ctcaatgata caggcaatac gtggataacc accggtggtc tgtgcgtcgt tcatcaacac
    35401 aatcggctgc ccgttatgcg gcacctgcac cacgcccggt aacaaaccgt gagataacag
    35461 ttcgcgatcg gtgatgcgtt ttaaaatttg cccctgtaaa cgatagccca ttcggttact
    35521 ttgcgagcta agctgccagg gcgaacgcca gaatgcatcc tgcgaggcgc gatcgaactc
    35581 atgatattcc ggccccggca aggcgcgaat gcggttgccc cacagcagct gtttaacgcc
    35641 ctgcgcttcc ataaaatcac gcttcgattt gccaatcggg agtcagtcac catccttcag
    35701 taaacggcct tccagcccgc caatccccac tttgagatcg gtgctgcatg accccattac
    35761 cggcggaaca tcaataccac ccgcgaccgc cagataactg cgcatcccgt gctgcgggcg
    35821 tttaagcgtt aaacgctggc ctgctttcat cggcaatcgc cagccggtcc agacggcgtt
    35881 atcatccaac cgcgcttcgc aacctgcacc cgtcagggca aaccacccat cagtttcgaa
    35941 ctcaactgtt aactgaccga gcgtgatctc cagcgcgggg gcattggcgt cattacccac
    36001 cagcaggtta gcaatgcgca gcgcaggcat atccagtgcg ccacagtggc tgatacccga
    36061 ctggcaaaaa ccgtgacgac cgccatcctg cacagtggtg tacatgcccg cacgaataat
    36121 cttcagcata ttccctcctt ctgcggtaca aatcgcacgc tgtctcccgg acgtaataag
    36181 atgggttcgt cacgcgccgg atcaaacagg ctgagtgagg tatgaccaat caactgccag
    36241 ccacccggcg ttgccagcgg ataaacacca gtctgcggcc cgccgatccc gacagaacct
    36301 gccggaacga gcaagcgcgg ttcagcgcgc cgtggcgtgt gtagttgttc cggcaaactc
    36361 ccgagatacg ggaagcccgg ttgaaaacct aaaaaccaga ccacgtattc cacggaggag
    36421 tgcaattcaa caacctgttt ttcgctcaac ccgcaatacg ccgcgaccac cgccaaatcc
    36481 ggtccgcctg caccaccgta aaccaccgga atttcaataa agcgagactc aggttccagg
    36541 gcctcgctct cctcccacca gcgttgcaaa cgctctatgg catccagcgc cagcgactca
    36601 ggattacgca aaatcaccgt gatattgttc atgccgggaa tggcttcaac cacat
//
LOCUS       DADLTY010000016        36408 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.016, whole genome shotgun sequence.
ACCESSION   DADLTY010000016 DADLTY010000000
VERSION     DADLTY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.016"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(24..278)
                     /locus_tag="JN334_000695"
     CDS             complement(24..278)
                     /locus_tag="JN334_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAY7436946.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="JN334_000696"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="JN334_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HAY7436947.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="JN334_000697"
     CDS             1228..2694
                     /locus_tag="JN334_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY7436948.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNALGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /locus_tag="JN334_000698"
     CDS             2812..3798
                     /locus_tag="JN334_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAY7436949.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="JN334_000699"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="JN334_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HAY7436950.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="JN334_000700"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="JN334_000700"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HAY7436951.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="JN334_000701"
     CDS             5709..7265
                     /locus_tag="JN334_000701"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY7436952.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="JN334_000702"
     CDS             7347..9161
                     /locus_tag="JN334_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7436953.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="JN334_000703"
     CDS             9162..10256
                     /locus_tag="JN334_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HAY7436954.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="JN334_000704"
     CDS             10256..11281
                     /locus_tag="JN334_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7436955.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="JN334_000705"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="JN334_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HAY7436956.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="JN334_000706"
     CDS             complement(12876..13220)
                     /locus_tag="JN334_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7436957.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JN334_000707"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JN334_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HAY7436958.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JN334_000708"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JN334_000708"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HAY7436959.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="JN334_000709"
     CDS             15616..17376
                     /locus_tag="JN334_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY7436960.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="JN334_000710"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="JN334_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HAY7436961.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JN334_000711"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JN334_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HAY7436962.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="JN334_000712"
     CDS             19113..19340
                     /locus_tag="JN334_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HAY7436963.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="JN334_000713"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="JN334_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HAY7436964.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="JN334_000714"
     tRNA            21195..21271
                     /locus_tag="JN334_000714"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="JN334_000715"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="JN334_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HAY7436965.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="JN334_000716"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="JN334_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HAY7436966.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /locus_tag="JN334_000717"
     CDS             complement(25143..26195)
                     /locus_tag="JN334_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HAY7436967.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="JN334_000718"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="JN334_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HAY7436968.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /locus_tag="JN334_000719"
     CDS             complement(26746..28689)
                     /locus_tag="JN334_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY7436969.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="JN334_000720"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="JN334_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAY7436970.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="JN334_000721"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="JN334_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAY7436971.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="JN334_000722"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="JN334_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HAY7436972.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="JN334_000723"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="JN334_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAY7436973.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="JN334_000724"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="JN334_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HAY7436974.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="JN334_000725"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="JN334_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HAY7436975.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="JN334_000726"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="JN334_000726"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HAY7436976.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="JN334_000727"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="JN334_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HAY7436977.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="JN334_000728"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="JN334_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HAY7436978.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..>36408)
                     /gene="napA"
                     /locus_tag="JN334_000729"
     CDS             complement(34052..>36408)
                     /gene="napA"
                     /locus_tag="JN334_000729"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HAY7436979.1"
                     /translation="PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLP
                     KIMYGKDRLTQPLLRMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMF
                     GSGQWTIWEGYAASKLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIE
                     QADAFVLWGANMAEMHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQ
                     SDLVILNYIANYIIQNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDA
                     SEPMSFEDYKAFVAEYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHT
                     RGVWANNLVYNLHLLTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNE
                     KHRDICEKKWNIPSGTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEER
                     MPGWRDPRNFIIVSDPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAP
                     GEAKSDLWQLVQFSRRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELA
                     EDQLNDESRELGFYLQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQW
                     RYSEGNDPYVKAGEGYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWH
                     TGSMTRRVPELHRAFPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNR
                     PPQGLVYMPFFDAAQLVNKLTLDATDPLSKETDFKKCAVKLEKV"
BASE COUNT         8881 a         9850 c         8984 g         8693 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgccctg
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atacaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgagg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ttttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggcc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gttaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcagtat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcg
//
LOCUS       DADLTY010000017        35709 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.017, whole genome shotgun sequence.
ACCESSION   DADLTY010000017 DADLTY010000000
VERSION     DADLTY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35709
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.017"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            375..814
                     /locus_tag="JN334_000730"
                     /pseudo
     CDS             375..814
                     /locus_tag="JN334_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(893..2590)
                     /locus_tag="JN334_000731"
     CDS             complement(893..2590)
                     /locus_tag="JN334_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HAY7436980.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(2910..4328)
                     /gene="dhaM"
                     /locus_tag="JN334_000732"
     CDS             complement(2910..4328)
                     /gene="dhaM"
                     /locus_tag="JN334_000732"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAY7436981.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(4339..4971)
                     /gene="dhaL"
                     /locus_tag="JN334_000733"
     CDS             complement(4339..4971)
                     /gene="dhaL"
                     /locus_tag="JN334_000733"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HAY7436982.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4982..6052)
                     /gene="dhaK"
                     /locus_tag="JN334_000734"
     CDS             complement(4982..6052)
                     /gene="dhaK"
                     /locus_tag="JN334_000734"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAY7436983.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6271..8199
                     /gene="dhaR"
                     /locus_tag="JN334_000735"
     CDS             6271..8199
                     /gene="dhaR"
                     /locus_tag="JN334_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HAY7436984.1"
                     /translation="MRDMSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGV
                     TFASIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSA
                     LGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRL
                     TGTIALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDG
                     VISWDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEA
                     TFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDD
                     PQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGD
                     AALAEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITR
                     LDARRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPAL
                     VNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSD
                     LPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTL
                     WRKMKQHGIDAGQFKRRV"
     gene            complement(8299..11166)
                     /locus_tag="JN334_000736"
     CDS             complement(8299..11166)
                     /locus_tag="JN334_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7436985.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(12010..13101)
                     /gene="ychF"
                     /locus_tag="JN334_000737"
     CDS             complement(12010..13101)
                     /gene="ychF"
                     /locus_tag="JN334_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAY7436986.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(13218..13802)
                     /gene="pth"
                     /locus_tag="JN334_000738"
     CDS             complement(13218..13802)
                     /gene="pth"
                     /locus_tag="JN334_000738"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY7436987.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            14080..14358
                     /gene="ychH"
                     /locus_tag="JN334_000739"
     CDS             14080..14358
                     /gene="ychH"
                     /locus_tag="JN334_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HAY7436988.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(14413..16092)
                     /gene="dauA"
                     /locus_tag="JN334_000740"
     CDS             complement(14413..16092)
                     /gene="dauA"
                     /locus_tag="JN334_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HAY7436989.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(16217..17164)
                     /locus_tag="JN334_000741"
     CDS             complement(16217..17164)
                     /locus_tag="JN334_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAY7436990.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(17315..18166)
                     /gene="ispE"
                     /locus_tag="JN334_000742"
     CDS             complement(17315..18166)
                     /gene="ispE"
                     /locus_tag="JN334_000742"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAY7436991.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(18166..18789)
                     /gene="lolB"
                     /locus_tag="JN334_000743"
     CDS             complement(18166..18789)
                     /gene="lolB"
                     /locus_tag="JN334_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HAY7436992.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            19003..20259
                     /gene="hemA"
                     /locus_tag="JN334_000744"
     CDS             19003..20259
                     /gene="hemA"
                     /locus_tag="JN334_000744"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAY7436993.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            20301..21383
                     /gene="prfA"
                     /locus_tag="JN334_000745"
     CDS             20301..21383
                     /gene="prfA"
                     /locus_tag="JN334_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAY7436994.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            21383..22216
                     /gene="prmC"
                     /locus_tag="JN334_000746"
     CDS             21383..22216
                     /gene="prmC"
                     /locus_tag="JN334_000746"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY7436995.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            22213..22605
                     /locus_tag="JN334_000747"
     CDS             22213..22605
                     /locus_tag="JN334_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HAY7436996.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            22609..23418
                     /locus_tag="JN334_000748"
     CDS             22609..23418
                     /locus_tag="JN334_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HAY7436997.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            23454..24308
                     /gene="kdsA"
                     /locus_tag="JN334_000749"
     CDS             23454..24308
                     /gene="kdsA"
                     /locus_tag="JN334_000749"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAY7436998.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24457..24564)
                     /locus_tag="JN334_000750"
     CDS             complement(24457..24564)
                     /locus_tag="JN334_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrA/LdrC"
                     /protein_id="HAY7436999.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(24969..26069)
                     /gene="chaA"
                     /locus_tag="JN334_000751"
     CDS             complement(24969..26069)
                     /gene="chaA"
                     /locus_tag="JN334_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HAY7437000.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            26339..26569
                     /gene="chaB"
                     /locus_tag="JN334_000752"
     CDS             26339..26569
                     /gene="chaB"
                     /locus_tag="JN334_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HAY7437001.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            26727..27422
                     /locus_tag="JN334_000753"
     CDS             26727..27422
                     /locus_tag="JN334_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY7437002.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(27466..27819)
                     /gene="ychN"
                     /locus_tag="JN334_000754"
     CDS             complement(27466..27819)
                     /gene="ychN"
                     /locus_tag="JN334_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HAY7437003.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            28004..29398
                     /gene="ychO"
                     /locus_tag="JN334_000755"
     CDS             28004..29398
                     /gene="ychO"
                     /locus_tag="JN334_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HAY7437004.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(29399..30049)
                     /gene="narL"
                     /locus_tag="JN334_000756"
     CDS             complement(29399..30049)
                     /gene="narL"
                     /locus_tag="JN334_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HAY7437005.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(30042..31838)
                     /gene="narX"
                     /locus_tag="JN334_000757"
     CDS             complement(30042..31838)
                     /gene="narX"
                     /locus_tag="JN334_000757"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HAY7437006.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            32177..33568
                     /gene="narK"
                     /locus_tag="JN334_000758"
     CDS             32177..33568
                     /gene="narK"
                     /locus_tag="JN334_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HAY7437007.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            33961..>35709
                     /locus_tag="JN334_000759"
     CDS             33961..>35709
                     /locus_tag="JN334_000759"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY7437008.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEK"
BASE COUNT         8763 a         8855 c         9118 g         8973 t
ORIGIN      
        1 attttattaa tgcatttctc attcgggtga aatttccgct acctggacgc ataaaaccgg
       61 aactctcgcc aaaagtcggg cactccgaag gaattgacgc gttgcgctgc tctgtaacgg
      121 catttaccgc aagccattta tttcgccagc gacaatccgc agagaaccaa acgcatcagg
      181 aaacgactga acacaataaa tactgtattg atcaacatat tatactggca cctccagcac
      241 gcagtgagga tacactgtat catcagcgaa tcttatcact gcggagctgg ttgaacgact
      301 ttctgagcga aataagtcgg ttcccggtaa caacaacaga atcctttttt aattattgtt
      361 tcgttgtttt tggtgtgatt cagaaaataa atattttaga cagtagccag tcaggcgtta
      421 aaaatggggc ggatagtgag gcggttttgg ctgcgcttat tgaacattta gaattgatca
      481 atccttctgg cagattaagc caaaataccc gttcggcaat gcttcagtta cgcgaagagg
      541 aatggtctga attctttttt tggttgctaa attctctgga gtgtctggat tatgtgatta
      601 ttaatttaac tccagaaagc aaaaaaacgt tgatgagtga acaccgtaac aatattcggt
      661 agcaattgat gctttatata gccaacgtcg gcgtaaatcg ccgggtgatg aaagtgaaac
      721 acttacccgt agaaatgacg ctatcttcgg caaccacgta tggcaaacat ttgcgcaata
      781 cttcccgcca ggacttgaaa aaccgtctgt gtagaaaaaa ccatagatat ggtgttatct
      841 gccgacaacg ccatccggag aagccgaatg acgttgtcgg agtaagcgct ggttaaggtg
      901 tgggttgtgc ctctttggtt gagggttgcg tcgttgctga cttaacggtc ggatgcgtcg
      961 ccggaacatt gtcacacggt tgctctttcg ggcagatcaa atccagcatt ttcagcgtca
     1021 cgccattggt ccagccaaag ccatcctgta atggatattc gccaccgccg ccccccgttc
     1081 cggtggtgct gacatcatat ttttccacca gctttttctc ccggtcatag gtgtgctgaa
     1141 cattggtcag gaagtgccag ctaatgtcca tcgccacctc tttttgcccg tagttttgta
     1201 atccttctgt cgcgacccac tgtaacggtg cccagccatt tggcgcatcc cattgttgcc
     1261 cacttttcac cgacgtggtg ttcaggccgc cgggttgcag cagatgtgtt ttcgtcgccg
     1321 tcgccatttt gttggcgcga tctttcgctg ccgcattgac gtacagcggg aacagggcgg
     1381 ccgcggttaa ctgattgcgc actttatgac ttttcaggtc gtaatcggca taccagcctt
     1441 gttgatcgtt ccacaggtat ttttcgatcc ctttttgacg ggcattcgcc agcgtttcgt
     1501 actggtttgc catcgcgtta tctcctgcag ctttgctggc gcgggcgagg attttttcca
     1561 ttttaaacat caggctgttc agatcgaccg gtacgatgct ggtggtgcgt aaggtattta
     1621 actgctgcgg gttgtccatc cagcgcgagc tgaaatccca gccagacgca gcggcagagc
     1681 gcaggtcgcg gtaaatttca gtggcaggtc gattcggatt gcttttggcg gtggcaatat
     1741 cttccaccca tgactctggt cgtggcgtat cgcgatcgtc ccagtagcgg ttgagaaggg
     1801 tactatcctg aagttttaca acgcgttttt cctgttgtcc ggcttgcagg ttttcaacac
     1861 cgtccatcca gtaagcatat tctttttgca tttgcggcag gtattgcttc aacgcggcat
     1921 cgccttcatg ctgcgccagt aactctacca tcagggcaaa gaagggcggt tgcgagcggc
     1981 ttaaatagta actgcggttg ccgttgggaa tatgaccgta agtgtttatt tcatgagcaa
     2041 aattggccac catatccgcg attttatccc agtgaccact ttcggcaagt cctaacatgg
     2101 tgaagtaatt gtcccagtaa tatacctcgc gaaaacgtcc gcccggcacg acataaggtt
     2161 ccggcagcgg taacagagaa tcccattttt cggtgttttc ggtagaacgc gttaataccg
     2221 gccaaagtcc gtcaatatgt tcgcgcagtg actgcccctc tggcggaaca tatttctcgc
     2281 cttctttcgg cagggtgaaa ttgacgttaa cgaaatggcg cagatcaaat ccgctctggt
     2341 tttgctgcat ccgataatca gcaaggatca tcagcggatc gctgttaggc acggcatcgg
     2401 caaaggtttt ttggtccgga aaaagtttga cgttttgcac atcattaaac agcggcccta
     2461 ataaaatatc aggcggctgt ggtgttaccg gtgtttcttc tgcctgcacc gatagcgcag
     2521 cgaaacacaa aaagatacag gctggaatta acgccatttt ttgcgggcga gaaggtgcgg
     2581 gggatttcat caatcattct cctttggcga aaccgaataa aacggtattc aagtatcaga
     2641 aaaccttagt tcaggatcgc actagctgca tgatcgaaat ctcattctgc gaacggtcag
     2701 gcattttaga aaagggaatt aaagggataa ataacggcga aatagagtga tcaaataacg
     2761 tctgtttatt agccagccag cgacatttgg ttcgcgatta gcgaagaaaa atcatcataa
     2821 attggtaaaa aatataacag gtagttaaaa aacatcagtg ctgggtaaat tgccggatga
     2881 catcagaacg atgccatccg aacggtggct taaccctgac ggttgaaacg ttgcgtttta
     2941 acgtccagcg ttagcgtttc ttctggttgt atcgcataca gtttctcacc ctgctggcaa
     3001 atccagccaa tccccagttc acgggcgatt agggcgctgt gggataccgg acttccggcg
     3061 ctaaggcaga taccttttac aaccgccgga tccagttgca gtactgtgga aggataaatg
     3121 ttctccgcca gtagaatagt tggcgagtta aactgcggga gttcttcttt cgtttgggtc
     3181 aggtggacca gggtgcgatg cagaagatcg tccacatcaa tatagcgagc ttgtagatat
     3241 tcatcatcca gttgctggta ttgctggcta agttctttaa gaacttgctg ccaggcatat
     3301 tctgccgtgc aatgttcatg ctgaaggagt tcgcttgccg ccgccagcag ttccggatca
     3361 cctaacagtg tatggtgacc agaaaagatt gcggcaatat cgtcaagccc gctggcttct
     3421 gctttcgctg ttaacgtcat cagatctaat aacgtgaagt caatagcctg gcgtaatcga
     3481 tcttgttctt cttccgcggt cagggttgat tttgcctgta ccgtacataa aactggttga
     3541 taataaaagg ctttacccga aacaggcaga acgggacgca gagtaggtgg agcgacttcc
     3601 tccgtttcac caaagttatc ttcagccagg tgacggaaag cgatcagtgc ctcttcagct
     3661 tctggccctt tcgcaatcag gcgcagcgta tcgttatagc gaacttgtag taacgcaatc
     3721 tggttaatac tctctggtgt gacgcatttg ccgttttttt ccagcaacat atcggcatta
     3781 aatgtcgata aggtataaac cagccgggag gccggacgta catgcaggcc gttacggttt
     3841 tttatgacca ccgccagaga acgggcttct tcatcgtacg gaggacatgt gtcagagatt
     3901 tcagtgtcgg aggacggtaa acccagttgt tcacgtttgg cttccagcgc atgcatagcg
     3961 tcaaagataa ctttgtcgat atccgccccc gaggccgcgc tgaccgttgc tgccagtgta
     4021 ccttcgacca acggcgcagc acacaaacgt acttttgcgg cgatctcggg agccagcaat
     4081 tccagcgcag tttcagcact caataatgcg ctacccatat ccatcatgac cagcacatgg
     4141 tcggcatcag caacagattc gatggcctcc atcactttga cggcatcggt accaatggga
     4201 ttttgtggat cgtcaattcc cgcggcaatg gcgattttac aactatcact cattaacatc
     4261 tgacgggcta attcaccgac accttctccc agtcggctgc tatgtgaaac tatgaccagg
     4321 tttaccatca ccaattcctt actcttttgc ggctaacgcc aacatttgca tcataaacat
     4381 caccgaggtc gcgccgggat cctggtgacc aatactgcgt tcaccgagat aactggcgcg
     4441 gcctttgcgg gcttgcatcg taatcgtact ttgtgcagcg gattcggcga tgctactggc
     4501 agcttcgagc gccaccggaa cagagagatt ttgctcgctg gactgacgta acgattccac
     4561 caccggcacc cacacatcac acatggtttt atcgccaggt tcggctttcc cgcgactgat
     4621 tacgccgtcc gcgccatcgc ggaacatctg ataaagctct tccagtgtca ggctttgccg
     4681 tgcctgggtc gcctgtgcgg cgcggataaa gaaggtaccg aacagcggac cactggcacc
     4741 accgacgctg gaaagcagcg tcataccggt atttttgaga atgaaaccga tatctttatc
     4801 tgcgatagca gggagttttt ccaccacttt gctaaagcct cggttcatat ttagcccgtg
     4861 gtcagcgtcg ccaatctcgc gatccagccc ggtaagatac tcgctctcgg tgttgaaaat
     4921 atcgccacaa cgagtgagcc agttaacaat ttgagttctg ctcagtgaca ttgctttctc
     4981 cttatttacc ccagttaagg gccggggtgt ggaccggggc gtcccagaga gccagcgttt
     5041 cgtcatcaac tttcagtaag gtgattgaga aaccggtcat atccagtgag gtgcagtacg
     5101 cgccaattaa attacgttcg atagtcaatc ccgcttgctg gcaacgtgtg gtcaggcggt
     5161 tatagacgcc gtacagctca gaaagcggag ttgcgccaag attgttaacc agtgcaatca
     5221 cccgatcgcc agactggagc ggttgtttgg tttgtggttc ttcctgccag ctgccttgtt
     5281 gataatccca gaaacgcaaa gtgcgatggt atgagccatg ttccagcagg gtgtcgaaca
     5341 tttcatcgac ggtttgatca agggaagaga aagggcggcg gtcaatacct ggctcaccat
     5401 gaatgcctac gccaaactcc atctcattat ccgcaagggt aaaagaaggt ttgcccgcgg
     5461 caggaacggt acaggcaccg agagcgatac ctattgagtg gccttgatta ttcaacttac
     5521 gccccagttc cgcacaggcg tccagtgagt cgccacgctc cgccgctgcg cctacgagtt
     5581 tttcaattaa tacggtgttg gcaacgccgc gtcgcccggc ggtataaaga ctgtctttta
     5641 ccgcaacgtc gtcatcaatg accacagtgg tcacttttac gccgctatcg tgcagtaact
     5701 cggtcgctgt ttcaaagtta agaatatcgc cggtgtaatt tttgataatc aacagtacac
     5761 cttcgccgcc atcaatttgc atggagcatt caaagatttt atcgggcgtc ggtgaggtga
     5821 aaatttcgcc cggacaggcc cccgaaagca tcccctgacc gatataacca cagtgcatcg
     5881 gctcgtgtcc gctgccgcca cccgacagca gggcgacttt tcctgcaaca ggggcatcag
     5941 ctcgggtgac atacaccgga tcctgatgca gtgtcagcga tggatgcgct ttcgccagtc
     6001 ctgccagttg ttcgtccagt acgtcttgca catcattgat caattttttc attattttgc
     6061 tccagcaatt acggtagggc atggatgatg ttcaacgaca cggcgaccac tgactgccga
     6121 tgaatccatt gtgcatcagg caagggaaaa gaaaattagc ccaaattatg tttcatagtg
     6181 aaacatatgc tttaatgaat gttccatatt gaaactttta cgtgtattaa tacttaaaat
     6241 tgcgagccgg aacacctttt gtcataaggg atgcgggata tgagtggcgc ttttaacaac
     6301 gatggtcggg gcatatctcc cttaattgca acctcctggg agcgatgcaa taagctgatg
     6361 aaacgggaga catggaacgt accacatcag gcccagggcg tgacatttgc ttctatttat
     6421 cggcgtaaga aagcgatgct gacgctcggg caggctgcgc tggaagatgc ctgggaatat
     6481 atggcaccgc gagagtgtgc gctgtttatc ctcgatgaaa ccgcctgcat tctcagccgt
     6541 aatggcgatc cgcaaacctt gcagcagcta agtgcactgg gattcaatga cggcacgtat
     6601 tgcgccgagg gaattattgg tacttgtgcg ctatcgttag cggctatctc tggtcaggcc
     6661 gtgaaaacga tggccgatca acatttcaaa caggcactct ggaactgggc cttttgtgca
     6721 acgccgttgt ttgacagcaa gggccgattg acgggaacaa tagcgctggc gtgtccggtt
     6781 gagcaaacta ccgcagctga tttgccgttg acgttggcaa tcgcccgcga ggtcggtaat
     6841 ttactgctga cggacagttt gctcgctgaa actaaccgtc atttaaatca acttaatgcc
     6901 ctgttagaaa gtatggatga tggcgtgatt agctgggacg agcagggtaa tttgcaattt
     6961 attaatgccc aggcggcgcg ggtcttgcgc cttgacgcga cggcaagtca gggacgggca
     7021 atcactgaac tcttaacgtt acccgccgta ttgcaacaag caataaaact ggcacatccg
     7081 ctcaaacacg tagaagcaac ctttgaaagc cagcaccagt ttattgatgc ggtgataacc
     7141 cttaaaccga taatagaaac gcagggaacc agctttattt tgttgctcca tcctgtggaa
     7201 cagatgcggc agttaatgac cagtcaatta ggaaaagtca gccatacctt cgctcatatg
     7261 ccacaggacg atccgcaaac ccgccgcttg attcattttg gtcgccaggc ggcgcgcagt
     7321 agctttcctg tcctgctttg tggagaagag ggcgtgggca aggcactgct aagtcaggca
     7381 attcataatg aaagcgagcg tgctgcaggt ccttatatcg ccgtcaattg tgagttatat
     7441 ggtgatgctg cgctggcgga agaatttatt ggtggcgatc gcacggacaa tgaaaatggc
     7501 cgtctgagtc ggctggaact ggcacacggc ggcacgctgt ttcttgaaaa gattgaatat
     7561 ctggcggtgg agttacagtc tgctttgctg caggttatca agcagggggt tatcacgcga
     7621 ctggatgcgc ggcgtttaat accaattgat gtcaaagtga ttgcaacaac gaccgcggac
     7681 ctcgcaatgc tggtggaaca aaatcgtttt agtcgccagc tgtattacgc gctgcatgca
     7741 tttgaaatta ccatcccgcc tctgcgtatg cggcgtggca gcattccggc gctggtgaat
     7801 aacaaattac gcagtcttga aaaacgcttc tctacgcggc tgaaaattga tgacgatgcc
     7861 ctcgctcgcc tagtttcttg tgcatggcca ggcaacgatt ttgaacttta cagcgtcatc
     7921 gagaatcttg ctctgagtag tgataacggg cgcattcgcg tcagtgattt acctgaacat
     7981 ctgtttaccg agcaggcgac agatgatgtc agcgccaccc gcctttccac cagtctgtca
     8041 tttgcggaag ttgaaaaaga ggcaattatt aacgcagccc aggtcacagg cggtcgcatt
     8101 caggaaatgt cggctttact tgggatcggc cgcactacgc tgtggcggaa aatgaagcaa
     8161 catggcattg atgcagggca gtttaagcgc cgggtatgaa agacagaaac gatttctgat
     8221 acatcagagt gatctgtatt tcattccggc gcacgctaac aattttcagc atcgtttaag
     8281 ggcttgtcta tcccgcactt aaaagctgaa gcgatatcct ccgttgactt gcttctgatc
     8341 aaagaggtta ccctgcgtgt aatccgcttc gagatagaat gtgtgctgtt tgttatactg
     8401 tgcactgact cccacgccgt tattccagcc attacctttg aaactgtact tctcccggga
     8461 gtcgttcaac agatattcgg tatcccctga aaactcgcgg atagcgccag tcttcacata
     8521 gacattcagc tggctgttgc ctgcggtgat gtcatacccc agtatcatgc tggcacgccc
     8581 cagcagcgat tcgtagtgat tcagatgtat attgaggcca ttactcgcct tcatagccat
     8641 ctcattctgg tggctgtatg taagctgggt ttgcggctct atatagaacc cataaccagt
     8701 aggggacagg ttgaacctct gcccggcttc cagggagatg ctcattccat tcgcagtgcc
     8761 gttggcgtta acgccgttgt tctgactgtc cagtacgtgg aaactatttt tctggcgcga
     8821 tgcttttata acgagatcgc tgtaaaaacc gttttgtgcc atgtaactgg cgtacattcc
     8881 catgtagtct gaacgtgcgg taccgtcgcc tccgctatag tccggcgatg catgtgttga
     8941 gtcaatatac agaccgacat acaacggcat tacatcagag agacgtttat ccccaccaaa
     9001 ctggataccg ctgtaaccca tgtcaaagcc gctcagtttg ccactggcaa aggagtccag
     9061 gcttccccca taactgcgca accagatatt accgtcttta ctctgatttc gcaagtcacc
     9121 catccgttgc atcagcgtac ggttttcaac atagttcaat aagtaaccga cattgagata
     9181 attgccgcca gcatctgcgg tcgtggtggg tttaggcgtg ggagcgggtt caggcgtagg
     9241 atcggggttg actatgggag gctgagcggg agctggtgtg ggttcaggat taggagtcgg
     9301 ttccggaact gtcccggaag cgtaaagctc ccagttagtg ccatttttac gcacatcgta
     9361 cagatatccc cccaactcaa cctgagaaga cgcgctgaac gaggccgcgc cgtcagtggt
     9421 ttttaccact gtcagaactt catttcccgt tgtggcctcg ctgccctggt tgcggatagc
     9481 caatacgtga ttaccagcac tactcccgct gatattcaat aaatcccctc tattattaac
     9541 gccattaccc tcgccaacaa catcagcacg cataataaag gtactgttac cgctcaggtt
     9601 ctctacgttt aatgtggtaa atgtgccggc agttgacccg tggctggcaa aatcgacagt
     9661 tgaatggctc agcgccagcg tgtcgagatt agaattactt gttacgttcc agacgctgtt
     9721 attcattgca acgtccagtt tcccgccatt gacattatcg ctgagggagg aacctgtcca
     9781 aaccgaccca gggtgcatat ccagattgat tagcccacct ttggaaagaa cgctaccgtt
     9841 gataagcata cgacccgagg cgttgatgcg gctggcggca taaccatcgt catgttgcgt
     9901 tgcgatggcc atctggtcgg gtgtactcat atcaatgacc agatcgctcg tgaggtcgat
     9961 ctggctgttg gtcatggcat aaatccctct ggctcctgcc gcgccggtga tagccaaact
    10021 gtctccggtt attctaccgc cgctgagcgc ccacaaaccc agcgccagac tgccattacg
    10081 atcaaccgta atatcggtat tttgcatgtt gataacagcc gttgccgtct gggccgaggc
    10141 accataagaa ccgccggaaa agatgctgtt tcgttgcgct gccgtgccag aaaaattgat
    10201 tgtcgcgtct gaactactgg tgacgaggcc accgccctgc gcggaagaaa tatggctatc
    10261 tgcaccgata gtggttgtac caccacgaac ttcgacgcca ttggccgcgg ctccagttac
    10321 atcaacagtg agtgcattcg cgctaacctg gccaaagctc cagaggccgt gagcattatc
    10381 gccattggtt ttaatggtac tgtttgttcc gagatcgaca acagagtttt tctgtacgtt
    10441 tatccccatg gcgctgtagc cctgaacatc gattgtcagg tctgtcgccg taaaacgcgc
    10501 agcaccattg gcgttattgc cgttgagacc accgatataa acacctgtac ttccatcggt
    10561 cttgatttta cttccgcttc caagatcgac actggtgcca tagtcattga tggttaggcc
    10621 tataccgttc gagttttcaa tggtgaattg agtggctgtt agtgttgagc tatgcccaat
    10681 aatgatgccg tcatcgttac tcttaatggt gctgcctgtg cctaagtcag catgggtata
    10741 gtcaccaatt aagttaatgc cgatggcaga ggtttgacca acaacatcta ctgtgagtcg
    10801 gttagctgtc agggagctgt ttttgccctg aataatgatt ccttttgcat atccagaggc
    10861 gtcgttgaca ttgacggtga catcattacc caggttgata ttgccaggcg tgtttcctgc
    10921 tggggtcatt acaccataga ggtttcctga tggatcggcg gtatctcctg taattctgtc
    10981 accatcatta atagtaatat tggtgccata ttgacttcct gtatcagcag ctatcagcag
    11041 gggggaaaag ataattgcat tcaaaccaat agcgccaaat tttatcagtt gaattgaggg
    11101 cgaacggatt tttaattcag cgagacgatc ggctttggta ttcccattgt gttgtttgat
    11161 gcccatacca ctcctatata gtacccaggc tatgggtcta tggattttgt cattttgttt
    11221 aagtcaatga ataatatcct cttatcatca tgatatctgt atggtgcaat catgatgagc
    11281 ttctctgaga ataattctct attaccgggc aaaatcagga acacaggaat taatatatat
    11341 ttaacccccc ccttacaaag gtgagtttat atcgagttat tccctaagtt gtcgttactt
    11401 attccaattc gttactatga atatggcaaa aatattacaa caaacagcgg ttaaaaaatt
    11461 tggtgaagat tttttgtaat aatcctaatt agttatggga ttttcgcttg atgtatctgc
    11521 ataacaaaaa caatatgcgc catttttgca ttactaataa gaaaaagcaa aaaacatcga
    11581 tattcatttt tgttcaatgg attttctggc ctatcgttat tattgtgcta ctggttatca
    11641 ttttcatctt gccaggatcg cacgtaatca cgttattcgg ggactttatc tgcgggatat
    11701 tacccttcag gtaagtcctt ttctcgccag catattggag cgagatcaat attgatcagc
    11761 tgtgaagagg gctgatgtgt agtggaacaa aaactgacgt tatatcactg ctaatgagat
    11821 atcagtcatc tgaacactta tgtctttaat tattctcgtg gttcactgta ggcaataagc
    11881 acaaaagtgt aggatgttac aagaatgatt aggactcggt gaaatgaaaa atccacgcaa
    11941 ttgcgtggat tttatatact tttgcgctct tcatgagatt tagcgaaacc tcatgagaca
    12001 ataaattaat tagacgttga aaaggaagtt catcacatcg ccatctttca cgatgtaatc
    12061 tttaccttct gcacgcattt tgcctgcttc tttcgcgcct tgttcacctt tgtaagtgat
    12121 gaaatcttca aacgagatgg tttgtgcacg gataaagcct ttttcaaaat cagtatggat
    12181 tttgcccgct gcctgcggcg cggttgctcc aaccggaatg gtccatgcac gcacttcttt
    12241 caccccagcg gtgaagtaag tttgcaggtt cagcagctta taaccggcac ggatcacacg
    12301 gttcaggccc ggttcttcca gcccaagctc ctgcataaac tcgtcacgtt cttcgtcgtc
    12361 cagttcggca atgtctgctt caacagcagc acaaaccgga accacaacag aaccttcttt
    12421 cgccgcgatt tcacgcacct ggtcaagata tgggttgttt tcaaaaccat cttcgttgac
    12481 gttggctatg tacattgttg gtttcagcgt caggaagctc aggtaacgaa tagccgcttt
    12541 ctcttcagcg cttaaatcca gcgcgcgcag catacctgcg ttttccagct ggggcaggca
    12601 tttttccagg accgccagct cagctttcgc gtctttatcg ccacctttgg ctttcttctg
    12661 tacgcgatga atcgcacgtt cgcaggtgtc gaggtctgcc agcgccagtt cggtgttgat
    12721 aacttcaata tcgtcagccg ggttaacttt gccggaaacg tgaatgatgt tgtcattttc
    12781 aaagcagcga acaacgtgac cgatcgcttc ggtttcacgg atgttggtca ggaactggtt
    12841 acccagacct tcgcctttcg atgcgccttt taccagaccg gcgatatcga caaattccat
    12901 ggtcgtggga agcgtacgtt gcggttttac gatttcagcc agttgatcca ggcgagggtc
    12961 aggcattggt acgacgcctg tgttcggctc aatggtgcag aatggaaagt tggccgcttc
    13021 aataccggct ttggtcagcg cgttgaacag ggtagatttc ccgacgttgg gcaaaccgac
    13081 gataccgcat ttgaatccca tgatttaaat caccttaata tcttaataat caacctgtta
    13141 ttgctaacag attgcagaaa tggaaataac tttgcctatt atacacggca ctcggcaaaa
    13201 atgccgcaga caacgactta ttgcgcttta aaggcgtgca atcggttcgt tgctttggtc
    13261 aagccatctg taaaccacat ttcagtacaa cgcgccgctt cgtcgatggc ttcatcaatt
    13321 aacttctgtt cactaacagg cggtttgcct aacacaaaac cgacaacttt atttttatcg
    13381 cccggatgac cgattccgat gcgtaaacgg tgaaagttag ggttattacc caatttactg
    13441 atgatgtctt tcagtccatt gtgaccacca tggccaccgc ccaacttaaa tttggcgacg
    13501 ccaggaggca gatccagttc gtcgtgggcc accagaattt cgtccggatt aatgcggaaa
    13561 aaactggcca tcgccgcaac ggctttgccg ctgagattca taaatgtagt cgggactaac
    13621 aggcggacat cttcgcttcc aagggtgact cgcgaagtat aaccaaagaa tttagcctct
    13681 tcgcgcagcg gagcgcgcaa acgctctgcc agtaagtcaa cgaaccaggc accagcatta
    13741 tgtcgcgttg cggcgtattc agcaccgggg ttcgccaggc cgacaatcaa tttaatcgtc
    13801 acgttttttt gtcctgagtg tgtacataac tggcgcgtag tttactggtt gcggccccgc
    13861 ttgacaaaaa actgcgtatc aaatgcagat aacgtaataa ttgcctgagt ggactattag
    13921 aaagtcaagg tgttcaggcg tttatttgta aagttttgtt gaaataaggg ttgtaattgt
    13981 gattacgccc gcacataacc cacagggtgt tgtctatact ttacacataa ggaagagggg
    14041 tattccctgt tacaacccag aaagttccgg aggtgacata tgaaacgcaa aaacgcttcg
    14101 atactcggta acgtgctcat ggggttgggt ctggtggtaa tggtggtcgg cgtggggtat
    14161 tcaatcctca accagttacc acagtttaat atgccccagt atttcgcaca tggtgcagtg
    14221 ctaagtattt tcgtcggtgc cattctctgg ctggcgggtg cccgtgttgg cgggcatgaa
    14281 caggtgtgcg accgttactg gtgggttcgt cactatgaca aacgttgccg ccgtagcgat
    14341 aatcgccgtc atagctaaca aataatgcag tttgctgacc agtcagtttg cgctgactgg
    14401 tcaatctcgt acttataaat ccgccatcgc cgcgcgacga ttcgggaaga acgcgaggcg
    14461 tcccgggatc ggttgaatgc ctgcgcgagc catagttcgc agtggctgga attccacgtt
    14521 gcacacgcgc agttcacatc cttcgggcag acgcttcaca aaacgctgga acgcatcaag
    14581 accaccagca tcaagtaccg gaacggcatc ccacttcaga atcacaatcc gtttgccttc
    14641 aagacgtgac tccaggtccg tgaataagcc ttcagcagca gcaaaaaaca gcgggccaat
    14701 aacgcgcaga accaggacat cgtctggaac atctacgact accggtgcca ggcgagtcat
    14761 acgtgcgata cgacgcataa acagcagcga tgccagcacg atccccacgc tgatggcaat
    14821 aaccatatca aacagcacgg tcagtgacat acacagcagc atgacgatga tgtcatcttt
    14881 cggcgcatga cgcagcaagt cgaccacttt gtgcgcttca ctcatgttcc acgccaccat
    14941 caacagcagg gctgccatgg cggaaagcgg cagccaggag agcagtggtg ccagtaccag
    15001 cagggcgaga ataaccagaa tagagtggat caccgccgag ataggggaag ttgccccggc
    15061 acggacgtta gcggcagaac gcgcgatggc agcagtagcg gtaataccac caaagaatgg
    15121 agcgatgata ttccccagcc cctgaccgac cagttcacta ttcgccttgt gcttagtccc
    15181 ggtcatacca tccagtacca cagcgcagag cagagattcg atcgccccga gcattgccat
    15241 tgagaatgcc gcaggcagca gggtgcgaat agaatcccag gttagcgtga attctgaatt
    15301 aggcagatcc cacggcagca ccagttgcgg cagcagttgc ggaataccgt taccctgaga
    15361 accatcggcc aggacgtagt ggaattgcga accgatggta gcaacatgtc cgccgagcag
    15421 gttaacaatc cccatcaccg cgcaaccagc cagcaaggcc ggaaggtgac cgggtaaacg
    15481 aatgcccaga cgcggccaga aaaccagaat gccaagcgtc acaatgccaa tggcagcatc
    15541 acccacatta atggtcggca gcgccataaa taatgcgccg actttttgta gataatgttc
    15601 cgggacatgg gccatttgca gaccgagaaa atctttaatc tgcatggtac cgatggtgat
    15661 cccgataccc gaggtgaaac ctaaggtgac ggaaaccgga atatactcaa tcagacgacc
    15721 aaagcgtgcc agccccatca gaatcaaaaa gattcccgac aacaaggtcg caaccagcag
    15781 tcctgccagt ccaaactgtt gcgaaacggg atagagaatt accacaaatg ccgcagtcgg
    15841 accggaaacg ctaaagcgtg acccacccgt tagagcaatg acaatccccg caacagctgc
    15901 ggtatataaa ccgtactggg gggccacacc actaccaata gccaacgcca tcgccagcgg
    15961 gatagcaata atcccgacgg ttatcccggc aatcaggtca cgggtaaacc gtgcggcagt
    16021 atatttttct ttccagcaaa cgtcgatcag agcgcggaaa ggcatcacat gtgaggaaaa
    16081 tattttgttc acaataatgt ttcatccgtg agcgcatcat ctgtcaacta aatggcaggt
    16141 gaaggaggca taggtcatac aaatggatat tacagacaaa aaaacccgcc gcagcgggtc
    16201 tttgagccgg gttcgattag tgttcgaaca tggcagagat cgattcttcg ttgctgatac
    16261 gacgaatcgc ttcggccagc atacctgaca gggtcagagt acgcacgttc ggcagtgatt
    16321 tgatttcatc gctcagcgga atggtatcgc agacaacgac ttcatcaatt acagagttac
    16381 gcaggttgtt cgccgcgttg ccagagaaga tcgggtgagt cgcgtacgca aatacacgtt
    16441 tagcaccacg ttctttcagc gcttcagcag ctttacacag cgtaccgcca gtgtcgatca
    16501 tatcatcgac cagtacgcag tcacgacctg caacgtcacc gatgatatgc atcacctggg
    16561 aaacgttcgc acgcggacga cgtttgtcga tgattgccat atcggtatcg ttcagcagct
    16621 tagcgatagc gcgggcacgc acaacgccgc cgatgtccgg agaaaccaca attgggttat
    16681 ccagattcag ctgcagcatg tcttccagca ggatcgggct accaaatacg ttatcaaccg
    16741 gaacgtcgaa gaaaccctga atctgttcag cgtgcagatc cactgtcagc acacggtcaa
    16801 caccgacgct ggagaggaag tctgcaacca ctttcgcagt gattggtaca cgagcggaac
    16861 ggacgcgacg gtcctggcgc gcatagccaa agtaggggat aacagcggtg atacgacctg
    16921 cggaagcacg acgcagggca tcaaccataa cgactaattc catcaggttg tcgttagtag
    16981 gggcacaagt ggactggatg atgaaaatat caccaccgcg tacattttcg ttaatttgta
    17041 cgctgacttc gccgtcgcta aagcgaccta cagcggcgtc gccgagtgaa gtgtacaggc
    17101 ggttggcaat acgttgtgct agtaccgggg tggcgttacc agcaaaaagc ttcatatcag
    17161 gcacgagaag aacctcaggc atgcgtccat tggtggaaag aatctgccga aaactgtgcg
    17221 ggccaggcat gatcctttcc aggcggtgta ttaaagagcg cgatgcaacg tctggaacaa
    17281 ggtgacgttg tcaccgaaac tcagcttgcc cggcttaaag catggctctg tgcaatgggg
    17341 aaagattaac gcctttcgcc acaaagccat tgagccattc cggggcttgc tctagcacct
    17401 ggcgggcttc agactctgta tcaaattcag caaagacaca ggcccctgtc ccagtcaggc
    17461 gtgacggggc gtattctaac agccaggaaa gcaccgcatc aacctcgcga aaacgttttc
    17521 ttgcgataac ctcgcaatca ttgctgaatt cacattttag caacgtttct attgaccttt
    17581 ttggcgtatt gcgcgggagt tcaggatctt taaaaatcac cggagtcgga atacttacac
    17641 cagggtgcgc caccagatac cacttctctg gcggatccac cggcgttagt atttcaccaa
    17701 cgccttcggc aaacgcggca tgcccccgaa caaagacagg aacatctgcg cccagcgtca
    17761 gccccatttc cgccagctca tccatgctta gcccgcattg ccagagatga tttaatgcca
    17821 ccaggaccgt cgcggcattg gatgaaccac cgccgagacc gccgcccatc ggcaaacgct
    17881 tgtcaatgct gatattcgca ccgcttcccg tcggaagacg cccgctgtct gccgcagttt
    17941 tcatcaacaa ccgcgctgcg cgaacgatca ggttatcttc atgttccacg ccttcaacgg
    18001 gcgttaacag acgaatatcc ccatcgtcac gaagctcaat gctgatggtg tcgccgtaat
    18061 caagaaactg aaacagcgtt tgcagcgtgt ggtaaccatc cgcacgctga ccggtaatgt
    18121 ataaaaacag attaagtttt gccggagagg gccactgtgt ccgcattatt tcactatcca
    18181 gttatccatt tttaacttga tgcgttgacc accgtcggtg agttccatat tggctggcat
    18241 cgcaggttgc gttttggtgt cataaccacc ataaacaacc ttccagtttt tgccattctg
    18301 gctgtaggta atttcgctca ggcggtactg gtcgtccagt ttgtagtcgg ttgcatcacc
    18361 cggtaaacct aaaatccact ggcgcaagct gttgagcgga attggcattc cggtcaattt
    18421 gccaatcatc tcttcggcgt catcggcggt ataacgctga cctttattgt cgactaactg
    18481 cacgttaccc ggttgagcat tcagctccag ttccgtgctg cccaatgggt tagtgagcag
    18541 cagacggtag cgatcctggc cggtttgctg ccagaaaaag cgggcgtaca ctttttgttg
    18601 gtcagaaata taagcgaacg cgccgcgagt ctgatactga ttaagattgc gcacgtcttg
    18661 ctgatgctga cgccattgtg gcgaatccgg gcttttgcca ggacctttgg gcgtggtaac
    18721 ggaacaggca gtgagcacaa gagcagccag cggtagcagg cggataagac gaaaatcggg
    18781 caggggcata gtgatgacaa gtccttgaga tacgttgcag ttataaccct taatgctagc
    18841 gttaccgtcc gctatcgtct atgttcaagt tgtcttaatt gccagaatct aacggctttc
    18901 ggcaattact ccaaaagggg gcgctctctt ttattgatct tacgcatcct gtatgatgca
    18961 agcagactaa ccctatcaac gttggtatta tttcccgcag acatgaccct tttagcactc
    19021 ggtatcaacc ataaaacggc acctgtatcg ctgcgagaac gtgtatcgtt ttcgccggat
    19081 aagctcgatc aggcgcttga cagcctgctt gcgcagccga tggtgcaggg cggcgtggtg
    19141 ctgtcgacgt gcaaccgcac ggaactttat cttagcgttg aagagcagga taacctgcaa
    19201 gaggcgttaa tccgctggct ttgcgattat cacaatctta atgaagaaga tctgcgtaaa
    19261 agcctctact ggcatcagga taacgacgcg gttagccatt taatgcgtgt tgccagcggc
    19321 ctggattcac tggttcttgg ggagccgcag atcctcggtc aggttaaaaa agcgtttgtc
    19381 gattcgcaaa aaggtcatat gaaggccagc gaactggaac gcatgttcca gaaatctttc
    19441 tctgtagcga aacgcgttcg cactgaaaca gatatcggtg ccagtgctgt gtctgtcgct
    19501 tttgcggctt gtacgctggc gcggcagatc tttgaatcgc tctctacggt cacagtgttg
    19561 ctggtaggcg cgggcgaaac catcgagctg gtagcgcgtc atctgcgcga acataaagta
    19621 cagaagatga ttatcgccaa ccgcactcgc gaacgtgccc aaattctggc agatgaagtt
    19681 ggcgcggaag tgattgccct gagtgagatc gacgaacgtc tgcgcgaagc cgatatcatc
    19741 atcagttcca ccgccagccc gttaccgatt atcgggaaag gcatggtgga gcgcgcatta
    19801 aaaagtcgtc gtaaccaacc aatgctgttg gtggatattg ccgttccgcg cgatgttgag
    19861 ccggaagttg gcaaactggc gaatgcttat ctttatagtg tggacgatct gcaaagcatc
    19921 atttcgcaca acctggcgca gcgtaaagcc gcagcggttg aggcggaaac tattgtcgct
    19981 caggaaacca gcgaatttat ggcgtggctg cgagcacaaa gcgccagcga aaccattcgc
    20041 gagtatcgca gccaggcaga gcacgttcgc gatgagttaa ccgccaaagc gttagcggcc
    20101 cttgagcagg gcggcgacgc gcaagccatt atgcaggatc tggcatggaa actgactaac
    20161 cgcttgatcc atgcgccaac gaaatcactt caacaggccg cccgtgacgg ggataacgaa
    20221 cgcctgaata ttctgcgcga cagcctcggg ctggagtagc agtacatcat tttctttttt
    20281 tacagggtgc atttacgcct atgaagcctt ctatcgttgc caaactggaa gccctgcatg
    20341 aacgccatga agaagttcag gcgttgctgg gtgacgcgca aactatcgcc gaccaggaac
    20401 gttttcgcgc attatcacgc gaatatgcgc agttaagtga tgtttcgcgc tgttttaccg
    20461 actggcaaca ggttcaggaa gatatcgaaa ccgcacagat gatgctcgac gatcctgaaa
    20521 tgcgtgagat ggcgcaggat gaactgcgcg aagctaaaga aaaaagcgag caactggaac
    20581 agcaattaca ggttctatta ctaccaaaag atcctgatga cgaacgtaac gccttcctcg
    20641 aagtccgtgc cggaaccggc ggcgacgaag cagcgctgtt tgcaggcgat ctgttccgta
    20701 tgtacagccg ttacgccgaa gcccgccgct ggcgggtaga aatcatgagc gccagcgagg
    20761 gtgaacatgg tggttataaa gagatcatcg ccaaaattag cggtgatggt gtgtatggtc
    20821 gtctgaaatt tgaatccggc ggtcatcgcg tgcaacgtgt tcctgctacg gaatcgcagg
    20881 gtcgtattca tacttctgct tgtaccgttg cggtaatgcc agaactgcct gacgcagaac
    20941 tgccggacat caacccagca gatttacgca ttgatacttt ccgctcgtca ggggcgggtg
    21001 gtcagcacgt taacaccacc gattcggcaa ttcgtattac tcacttgccg accgggattg
    21061 ttgttgaatg tcaggacgaa cgttcacaac ataaaaacaa agcgaaggcg ctttcagtgc
    21121 tcggtgctcg catccacgct gctgaaatgg caaaacgcca acaggccgaa gcgtctaccc
    21181 gtcgtaacct gctggggagt ggcgatcgca gcgaccgtaa ccgtacttac aatttcccgc
    21241 aggggcgcgt taccgatcac cgcatcaacc tgacgctcta ccgcctggat gaagtgatgg
    21301 aaggtaagct ggatatgctg attgaaccga ttatccagga acatcaggcc gaccaactgg
    21361 cggcgttgtc cgagcaggaa taatggaata tcaacactgg ttacgtgaag caataagcca
    21421 acttcaggcg agcgaaagcc cgcggcgtga tgctgaaatc ctgctggagc atgttaccgg
    21481 cagagggcgt acttttattc tcgcctttgg tgaaacgcag ctgactgacg aacaatgtca
    21541 gcaacttgat gcgctactga cacgtcgtcg cgatggtgaa cccattgctc atttaaccgg
    21601 ggtgcgagaa ttctggtcgt tgccgttatt tgtttcgcca gcgaccttaa ttccgcgccc
    21661 ggatacggag tgtctggtgg agcaggcact ggcgcggttg cctgaacaac cttgccgtat
    21721 tctcgatctc gggacgggta ccggggcgat tgcgcttgcg ctggctagcg agcgcccgga
    21781 ctgcgaaatt atcgctgtag atcgtatgcc tgatgctgtc tccctggcac aacgtaatgc
    21841 ccagcatctg gcgatcaaaa atatccacat tctgcaaagc gactggttta gcgcgctagc
    21901 cgggcagcag tttgcgatga ttgtcagcaa tccgccgtat attgacgagc aggacccaca
    21961 tcttcaacaa ggcgatgtcc gctttgagcc gctcactgcg ctggttgcgg cagacagtgg
    22021 aatggcagac atcgtgcata tcatcgaaca gtcgcgtaac gcgctggtat ccggcggctt
    22081 tctgcttctg gaacatggct ggcagcaggg cgaagcggtg cgacaagcat ttatcctcgc
    22141 ggggtatcat gacgtcgaaa cctgccgtga ctatggtgat aacgagcgcg taacgctcgg
    22201 ccgctattat caatgacaag tttttctaca ctgcttagtg ttcatcttat tagtatcgcg
    22261 ctttctgttg ggctattaac cttacgtttc tggctacgtt atcagaagca tccacaggca
    22321 tttgctcgct ggacgcgcat tgtgccgccg gttgtcgata cggtgttatt gttaagcggc
    22381 attgcgttga tggctaaagc gcacatcctg ccattttccg ggcaggcaca gtggctgact
    22441 gaaaagctgt ttggagttat catttatatc gttttgggtt ttattgcact cgattatcgt
    22501 cgtatgcaca gtcagcaggc gcgcattatt gccttcccgc tggcgttggt ggtgctgtac
    22561 atcatcatta aactcgccac cacaaaagta ccgttactgg ggtaagtcat gagatcgtta
    22621 gctgatttcg aatttaataa agcgccattg tgcgaaggca tgatcctggc ttgcgaagca
    22681 atccgccgcg attttccctc gcaagatgtt tacgacgaac tggagcgtct cgttagtctg
    22741 gcgaaggaag aaatcagcca gcttctgcct ttagaagagc agctggaaaa actgatcgcg
    22801 ctgttttacg gcgactgggg atttaaagcc tcacgcggcg tttatcgtct ttccgatgca
    22861 ttatggctgg accaggtgtt aaagaatcga cagggcagtg cggtatcatt aggtgcggtt
    22921 ttattatggg tcgcgaatcg tctcgatttg ccgctgctgc cggtgatttt ccctacgcag
    22981 ctgatattgc gcattgaatg tccggatggc gaaatttggc tgattaatcc ttttaacggt
    23041 gaatcgttaa gcgaacatat gctggacgga tggttaaagg gaaatatcag cccgtcggcg
    23101 gaactgtttt atgaagacct tgatgaagct gataacattg aggtaatccg caaattgctg
    23161 gatacactca aagcctcgtt gatggaagaa aatcagatgg agctggcgtt acgcaccagc
    23221 gaagctttat tacaattcaa ccctgaagat ccctatgaaa ttcgcgatcg tgggttgatt
    23281 tatgcgcaac tggattgcga acacgttgcg ttgaacgatt taagttattt cgttgaacgg
    23341 tgtccggaag acccgatcag cgaaatgatc cgtgcgcaaa taaataacat cgcgcataaa
    23401 catattgtgc tgcattaatt aatcgacatt ttactcaaga ttaaggcgat cctatgaaac
    23461 aaaaagtggt tagcattggc gacatcaacg tagcaaatga cctgccgttc gtactgtttg
    23521 gcggtatgaa cgtgctggaa tctcgcgatc tggcgatgcg catttgcgag cactacgtaa
    23581 ctgtgaccca gaaactgggt atcccttacg tgttcaaagc ctcttttgac aaagccaacc
    23641 gctcctccat ccactcttat cgtggaccgg gcctggaaga agggatgaaa atcttccagg
    23701 agttgaagca gacttttggc gtgaaaatta tcaccgacgt tcacgaacca agtcaggcac
    23761 agcccgttgc tgatgtcgtg gatgtgattc agttgccggc gtttcttgct cgccagactg
    23821 acctggttga agccatggcg aaaaccggtg cggtaattaa cgtcaagaaa ccacagtttg
    23881 tcagcccggg acagatgggt aatatcgttg ataaattcaa agaaggcggc aacgaaaaag
    23941 tgattctttg cgatcgtggt gctaacttcg gctatgacaa cctggttgtc gatatgctgg
    24001 gcttcagcat tatgaagaaa gtgtctggta actcgccggt gattttcgac gtgacccacg
    24061 cactgcaatg ccgcgatccg tttggcgcag cttccggtgg tcgtcgtgct caggtggctg
    24121 agctggcacg agccggtatg gcggtaggtc tggcggggct gtttattgaa gcgcatccgg
    24181 atccggaaca tgcgaaatgt gatggtccat ccgcgctgcc gctggctaaa ctggaaccgt
    24241 tcctcaagca gatgaaagcg attgatgatc tggtgaaagg tttcgaagaa ctggatacca
    24301 gcaagtaatc ttttttgctt gaaaaataaa gtcttagcgt tctgcgttaa gacttttttc
    24361 atgggtgcca gatacaaaaa aggccgcagg ctgttacccc tgcggccggt ttcgggcgca
    24421 tattgccatc acggcagcct gacgcccgtt ttcaccttac ttccggttac gccaccagct
    24481 gacaatcgct gcggcaataa tccccgccag gatcggtgct gccaggtcgt gccagaaagt
    24541 catggcaaac tgcgcgagcg tcatatagcc gccttgttgt aatgacaaca ttttgcggct
    24601 attcttgaag tgtctggttt caagattagc ccccgttttg ttgtcaggtt ttacctctca
    24661 acgtgcgggg gttttctctt tccagcaacc aatgccacca gggataaagc ccccgcaaca
    24721 ttgcgcctca ccggataatt ccggcttggt gtggatacta cgtctcaatt catcttcact
    24781 tcatccctga aatgtttgca ataaagagta cattccggct tttcaacagc tgttgcagtg
    24841 gtttcgtgca ggttgtggta caggctcgca attctgatta acgagctgtc caggtagtat
    24901 gaagaaatac aataagcagg aatttatctt atctctcccg atttccgtca tcggaggaga
    24961 tataactgtc aggcaaatat cgtcatcaaa taggcggcaa acagtgccag atgcgctgcg
    25021 ccattgagca cgttagtacg tccggtggag aaggagatat ggcacagcac taaagaggcc
    25081 accatcacca ccatttctgg cgcaccaagt gcaaactgca attcgttacc cgtcataaag
    25141 gcaattagcg tgacgacagg tacggtaagc gaaattgttg ctaacactga accaaagaac
    25201 agattcatcg cgcgctgaac ctggttgttc aacactgctt ttaacgcacc taaaccttcc
    25261 ggcgacagaa tcaacagtgc caccaggaag ccagtaaagg caacaggggc attcatgctg
    25321 tcgagcaatg tctccagcga gctggcgttc attttggtga ctgcaataac ggcaatcaga
    25381 tggataatca accagatagc atgccacagg ctgctatggg cagacgtttt accgtggtgc
    25441 gggtcgtcgt catcactgtc atcttcgtgc tcgtagacaa acaaactttg atgcgttttg
    25501 gcctggatca gcaaaaatac gccatacatt gccgcagaaa ttaatgctac cagtaacgcc
    25561 tgaccggttg aaaaattcgc cgcaggcaga gccattggaa ataccagtac gattatcgcc
    25621 agggggaaca gcgcaattaa atactgcttg ataccaaaca gattcatata ttgggtggca
    25681 aacttacgac cgcccaacaa taatgaaaag ccaaccagcc caccggtaac aatcataatg
    25741 attgaataga gcgtatcacg cattagcgtt ggcgcggcgt cgccggttgc cattaaagct
    25801 gaaatcaaac tgacttcaag aataaccact gaaaggctaa gaataagcga accgtaaggt
    25861 tctcccaggc gatgggctaa tacgtccgca tgacggacaa cactaaaggc gctacttaaa
    25921 ataccaataa gcgcaagaag attgatggca atgaccactg gtagtgtctg gctacttccc
    25981 cacaggaaca gcactaccag caccagaacc gggaaaataa gcgaagtctc cttgtggcgg
    26041 gtttttaccg cctcttgagc atttgacatt atggttatcc ctttgcagat gaatttatcg
    26101 aaaatgtaaa aaataggtag gaaaaataac agaaagtgtc tggatatcgg taacatttta
    26161 cgaattttta ccccgctgtc gattttttac tcattggggc ataaaaataa gtattacgtt
    26221 tagacaatgt ttgttttagc gtttcttaag aacagtctga catgaaaatt cttatgtttt
    26281 ggcaagagta gatattgttg accacactta atgttcaact ttgtaaaagg agtcaacgat
    26341 gccgtataaa acgaaaagcg atctgccgga aagcgtaaag cacgttctac cgtctcatgc
    26401 ccaggatatc tataaagaag cgttcaacag cgcatgggat caatataaag ataaagaaga
    26461 tcggcgtgat gacgccagtc gcgaagaaac cgcgcataaa gtggcctggg ctgctgtgaa
    26521 gcatgaatat gccaaagggg atgatgataa atggcataaa aaatcgtaaa accggtagct
    26581 tagttaaagc tattcgtgcg gtgttgcctt gcaagtggtc cgtggattgc atattgttcc
    26641 gttagtggtt tcaaaatgag cggtaaaaat gtccggaaga caccaaaaag ttgtcgcagg
    26701 gaagtatgca gtggcggagg tgtaaggtga taacgcgtga tttcttgatg aatgccgatt
    26761 gtaaaacggc atttggtgcc attgaagaat cactcttatg gtcagcagaa caacgggcgg
    26821 cttcgctggc ggcgacgctg gcttgtcgac ctgatgaggg accggtgtgg atcttcggtt
    26881 atggatcgtt gatgtggaat ccggcactgg agtttaccga atcatgcacc ggtacactgg
    26941 ttggatggca tcgcgcgttt tgcctgcgcc tgaccgccgg gcgcgggact gcgcaccagc
    27001 cgggacggat gcttgcactg aaagagggcg gacgcaccac aggcgtcgcc tatcgactac
    27061 cagaagagac gctggagcag gaactaaccc tgttgtggaa gcgcgagatg attaccggct
    27121 gttatctgcc aacctggtgt cagcttgatc ttgatgatgg acgcacagta aacgccattg
    27181 tgtttattat ggacccgcga catccagaat atgaatctga cactcgcgct caggtcatcg
    27241 cgccgttgat tgcggcggcg agcggtccgc tgggaaccaa tgcacaatac ctgttttcac
    27301 tggaacagga gcttatcaaa ctgggaatgc aggacgatgg gctgaatgat ttgctggtat
    27361 cggtaaaaaa actactggcg gagaattatc cggatggtgt gttaaggccg ggattcgcct
    27421 gagtaaactt ccctcatagt ggggcgtcag acgccccctt aaacattaaa atgtgagcac
    27481 tttatcggct gacagcgtcc attgcgccag ttccacaaga gtaccgattt ccaccccatc
    27541 aatcagagga agtgtgctaa tcccgcgccc gtcggtacag gttttgcaca atttcaccgg
    27601 tacattctga gcggtaagga tctccagcat ttgctgaatg ttgtagcctt cccctggttt
    27661 ttgcccgcgc aacccggctg tgaccgcatc agacatcagg aacagacgca gatccagatt
    27721 gctctcctgc tctcgtaacg caatggccag ccgcaagctg ttaaacaagg attcgctccc
    27781 gtaaggtgcg ccattggcaa cgatcactat tttttgcatt atttactcct gtattcaggg
    27841 aattagacac tcatcttcta tcttactgct tctgcagcgt ctgaccaatc ggtcacattt
    27901 ttaaggattt tcctgaaagc gcgagaaaat acgacaaaag ttgccagtaa tcgttattct
    27961 ttaaggctat ggtttttcat tattaccgga agttaccgac gttttgagcc gtttcgttcc
    28021 tcgcattatc ccgttttatt tactcttgct tgtagcaggc ggtacagcta acgcacaatc
    28081 taccttcgag caaaaagcgg caaatccctt tgataataac aatgatggtc tgccggattt
    28141 aggcatggct cccgaaaatc atgatgggga aaaacacttt gctgaaattg tgaaagattt
    28201 cggcgaaacc agtatgaatg ataacgggct ggatactggc gagcaggcaa aagctttcgc
    28261 attgggaaaa gtccgcgacg cgcttagtca acaggttaat cagcacgtag agtcctggct
    28321 atcaccgtgg ggaaatgcca gtgttgacgt caaagtggat aacgaaggac atttcaccgg
    28381 cagtcgtgga agctggtttg tgccgttaca agataatgat cgttatctca cctggagcca
    28441 gcttggtctt actcagcagg ataatgggtt ggtgagcaat gtgggcgttg ggcaacgctg
    28501 ggcgcgcggc aactggctgg tgggttataa cactttttat gacaacttgc aggacgaaaa
    28561 tcttcagcga gcgggctttg gtgccgaagc gtggggcgaa tatttgcgat tatcggcaaa
    28621 cttttatcag ccgtttgctg catggcatga acagacagcc acgcaggaac aacggatggc
    28681 gcgcgggtac gacctgacag ctcggatgcg catgccgttc tatcaacacc tcaataccag
    28741 tgtcagccta gaacagtatt ttggtgatcg tgttgatttg tttaactctg gtacgggtta
    28801 tcacaatccc gtcgcgttga gtctgggatt aaattacacc cctgtgccat tagtcactgt
    28861 gacggcccag cataaacagg gggaaagtgg cgaaaatcaa aataacctcg ggctgaatct
    28921 taattaccgc tttggtgtac cgctcaaaaa acaactttct gcgggcgagg ttgccgaaag
    28981 tcagtcgtta cgtggtagtc gctatgacaa tccgcagcga aataatctac cgactcttga
    29041 gtaccgacag cgaaaaacgt taacggtgtt tctggcgaca ccgccgtggg atctaaaacc
    29101 tggcgaaaca gtgccgctga aattacaaat ccgcagtcgt tacggtattc ggcaactgat
    29161 ttggcagggc gatacgcaga tattaagttt gacgccgggc gcacaagcca acagcgcgga
    29221 gggctggacg ctgatcatgc ctgactggca gaacggggaa ggggcaagca atcactggcg
    29281 attgtctgtg gtggtggaag ataaccaggg gcagcgtgtc tcctccaatg agatcacgct
    29341 aacgcttgtc gaaccgttcg atgcattgtc aaacgacgaa ctgcgctggg aaccgtaatc
    29401 agaaaatgcg ctcctgatgc acccataccg ctgcttccac gcgagacttg agcttcattt
    29461 tcttcagcat gtgcttgacg tgcactttta ctgtgctttc ggtgatatcc aggcggcggg
    29521 caatcatctt gttcggcaaa ccctgggcaa tcagcttgag aatatcgcgc tcgcgtgggg
    29581 ttaactggtt aacatcgcgc tcagtagtgg cacggttagc gcgcaagctg gcggccagaa
    29641 caggcgttaa tgcttcgctt aataccattt cgccagcagc tgcctgatgc aatgctttca
    29701 gcagatcttc cggttccata tcttttaaca gatagccatc cgcgccgcgt ttcagtgcgg
    29761 tgaccacatc ttcttcatgg ttagagacgc tgaataccac aatgcgccct gagagggact
    29821 tttcgcgcag tttatccagc gtttccagac cgttcatgcc gggcatattg agatctaaca
    29881 ggatcagatc gggatcaaga gattccgcca gttcaatacc ctgttcgcca ttactcgctt
    29941 cgccaaccac ggtgatatct ggtgccatac tgataagctg ttttacgcca gttcgcagca
    30001 tcgggtgatc gtcaatcagc aggatagtag ccggttcctg attactcatg ggtatctcct
    30061 tggacgtctg tgaaagtttt ttcgggaata aaggtgacca ccacttcggt gccacctgat
    30121 tcacgacggc ggacgcggca atcgcctcgt aaactttgcg cacggtcgcg cattattatc
    30181 atgccgtagt gattgctgcg gatggcattt tcaggcacgc cacagccgtt atcctggacg
    30241 gtcagtttga cctgattatc gttttgcgcc accgtcacca cgacctcact cgcttgcgaa
    30301 tgtttgaggg cgttacttaa tgcctcacgg gcaatttgca acaagtggat tgcctgatgt
    30361 gaaggcacca gacgaggcgg caattgataa tccagcttca ccgggaagcc aaatttggcg
    30421 ctgtactctt cgcaactcgc ttccagcgcc ggacgtaatc caggctcggt gagctgcaag
    30481 cggaatgtgg tgagcaattc acgcaactgc gcccaggatg cattcagttc gttacggatc
    30541 tgacttaaca gttcgcggct gctttctggc agcgcatcgc cctgcatctg taaacaactc
    30601 acctgcatct tcatgcaaga gagagattgg gcaatagaat catgcagttc gcgcgcaatg
    30661 gtggcacgct cttccatcac gatcaactgt tgctgacgtt cctgatggcg atccagcgcc
    30721 agcgtggcgg taagttgttc aaccagggta tccaccagtt gttgttggtc atggctaaga
    30781 tgacgcccct gcggcagggt cgccagcaaa ataccgtact gcgtatgaga gtcagccagc
    30841 cgccacttca gggtcgtgcc gcgatcacca acgggtaata cgccgcgcgg gcagagctgg
    30901 cagcctttat catcacaagt catatctggc tggcaggtaa actcctgatg attctcttca
    30961 tcatcagtgt catacacccg cagttcgata tcacgtagca gggttaaatt ctgtaagccg
    31021 ttgagtaccg gtgacaggcg ttcacacagc ggggcgcggg aatgcaaacg gcggttagcc
    31081 tgccataaaa aagagaggat ctgattttta tgctccagcc cggcggtttt ctcctgaacc
    31141 cgctgctcaa gtacggcata actttcggcc agttctgcag acatattgtt caacgcagtt
    31201 ccaagcatcg ccatttcgtt gcgcccgctg atgtttgcgc gttgggtaaa atcgcgatgg
    31261 ctgacggcac tcgccattgc cagcagttgc cgccacggtt gtagcagtcg cgcccgcaac
    31321 cagataatag tgaacaccag taaaagtgcc ataaataccg ccattacccg atggaccagt
    31381 accactgtct caatacgcat ttccgtggtg cggtcaaaac cagataccag ctgatcaagc
    31441 ccggcaacaa actggctgac atccgctgac accgtttctc ggttttgtgc acgcatcagc
    31501 gcagggatca gttcattacg ccagtaatct tgtaagccct gtaattgcgc cagttgtccg
    31561 tctcgttctg ctgctcgagt caactcggcg ctaaatgccg tttgttccat ctctttaatt
    31621 aagggcttgt ctttctcgct taatggcact gccgccaaca gacggtaact ttgcatgcgc
    31681 agcgatcccg ctttgttgat cgcatgggcg ctgccctgaa cgccttgcac cagccagcca
    31741 gaaaccgcca tccctgccag tccaatagca gtagaaagca acacaataag cgcaacctga
    31801 ttaaccaggg tgagcggaga gagacaacgt ttaagcatgt aaaccccttc cttcaggctt
    31861 taaatgagca ataaccttaa tgaatgtgac gatatattct ggaatggcag tattctcggc
    31921 tattggctga agtataccca tagccggaaa gagttactcc ttatttgccg tgtggttagt
    31981 cgctttacat cggtaagggt agggattcta cagcaccgtg aaaaatctca taatttttat
    32041 gaaatcactg tactcactat gggtaatgat aaatatcaat gatagataaa gttatcttat
    32101 cgtttgattt acatcaaatt gcctttagct ccagacacta aggtggcaga catcgaaacg
    32161 agtatcagag gtgtctatga gtcactcatc tgcctccgaa agggctactg gagctgtcat
    32221 aacagattgg cgaccggaag atcctgcgtt ctggcaacaa cgcggtcaac gtattgccag
    32281 ccgcaacctg tggatttccg ttccctgtct gctgctggcg ttttgcgtat ggatgttgtt
    32341 cagcgctgtt gcggtgaact taccgaaagt cggttttaat tttacgaccg atcagctatt
    32401 tatgttgact gcgctgcctt cggtttctgg cgcgttatta cgtgttccat actcctttat
    32461 ggttcctatc ttcggtggtc gtcgctggac ggcgttcagc accggtattc tgattattcc
    32521 ttgcgtctgg ctgggttttg ccgtgcagga tacctccacg ccttatagcg tcttcatcat
    32581 catctctctg ctatgcggct ttgctggcgc gaacttcgca tccagtatgg caaacatcag
    32641 cttcttcttt ccgaaacaga agcagggtgg cgcgctgggt ctgaatggtg gtctgggaaa
    32701 catgggcgtc agcgtcatgc agttggttgc tccgctggtg gtatcactgt cgattttcgc
    32761 agtatttggt agccagggcg tcaaacagcc ggatgggact gagctgtatc tggcgaatgc
    32821 gtcctggata tgggtgccgt tccttgccat cttcaccatt gcggcgtggt ttggcatgaa
    32881 cgatcttgct acctcgaaag cctccatcaa ggagcagttg ccggtactca aacggggtca
    32941 tctgtggatt atgagcctgc tgtatctggc aaccttcggc tccttcatcg gcttctccgc
    33001 gggctttgcg atgctgtcaa aaacgcagtt cccggatgtt cagattctgc aatacgcttt
    33061 cttcgggccg tttattggtg cgctggcgcg ttctgcaggt ggtgcattat ctgaccgtct
    33121 gggcggaact cgtgtcacgc tggtgaactt tattctgatg gcgattttca gcggcctgct
    33181 gttcctgacc ttaccgactg acgggcaggg cggaagcttc atggcgttct tcgcggtctt
    33241 cctggcgctg ttcctgacag ctgggctggg tagtggttcc actttccaga tgatttcagt
    33301 gatcttccgt aaactgacaa tggatcgcgt gaaagcagaa gggggttctg acgaacgtgc
    33361 gatgcgtgaa gcggcaaccg gcacggcagc ggcgctgggt ttcatctctg cgattggcgc
    33421 gattggtggc ttctttatcc cgaaagcgtt tggtagctcg ctggcattaa cgggttcgcc
    33481 agtaggtgca atgaaagtat ttttgatttt ctatatcgcc tgcgtagtga ttacttgggc
    33541 ggtatatggt cggcattcta aaaaataaat cttaatattt gattatcctt tgcgcggcat
    33601 gatgccgcgc tttttttatg cgtcatttag ttacaacata ttagtgttat atggcttatt
    33661 tcgccggatt tcattaagag ccattaatat gttacccatg gggaatactc cttaataccc
    33721 atttgcataa aaatcttaat agtttaaata actataggta taaaacgtct taatttacag
    33781 catgttatgc tgtaactgtt aattatccta aaggggtatc ttaggaattt actttatttt
    33841 tcatccccat cactcttgat cgttatcaat tcccacgctg tttcagagcg ttaccttgcc
    33901 cttaaacatt agcaatgtcg atttatcaga gggccgacag gctcccacag gagaaaaccg
    33961 atgagtaaat tcctggaccg gtttcgctac ttcaaacaga agggtgaaac ctttgccgat
    34021 gggcatggcc agcttctcaa taccaaccgt gactgggagg atggatatcg ccagcgttgg
    34081 cagcatgaca aaatcgtccg ctctacccac ggggtaaact gcaccggctc ctgcagctgg
    34141 aaaatctacg tcaaaaacgg tctggtcacc tgggaaaccc agcagactga ctatccgcgt
    34201 acccgtccgg atctgccaaa ccatgaacct cgcggctgcc cgcgcggtgc cagctactcc
    34261 tggtatcttt acagtgccaa ccgcctgaaa tacccgatga tgcgcaaacg cctgatgaaa
    34321 atgtggcgtg aagcgaaggc gctgcatagc gatccggttg aggcatgggc ttctatcatt
    34381 gaagacgccg ataaagcgaa aagctttaag caggcgcgtg gacgcggtgg atttgttcgt
    34441 tcttcttggc aggaggtgaa cgaactgatc gccgcatcta acgtttacac catcaaaaac
    34501 tacggcccgg accgtgttgc tggtttctcg ccaattccgg caatgtcgat ggtttcttac
    34561 gcatcgggtg cacgctatct ctcgctgatt ggcggtactt gcttaagctt ctacgactgg
    34621 tactgcgacc tgcctcctgc gtctccgcaa acctggggcg agcaaactga cgtaccggaa
    34681 tctgctgact ggtataactc cagctacatc atcgcctggg gttcaaacgt gccgcagacg
    34741 cgtaccccgg atgctcactt ctttactgaa gtgcgttaca aaggcaccaa aactgttgcc
    34801 gtcacaccag actacgctga aatcgccaaa ctgtgcgatc tgtggctggc accgaaacag
    34861 ggcaccgatg cggcaatggc gctggcgatg ggccacgtaa tgctgcgtga attccacctc
    34921 gacaacccaa gccagtattt caccgactat gtgcgtcgct acaccgacat gccgatgctg
    34981 gtgatgctgg aagaacgcga cggttactac gctgcaggtc gtatgctgcg cgctgctgat
    35041 ctggttgatg cgctgggcca ggaaaacaat ccggaatgga aaactgtcgc ctttaatacc
    35101 aatggcgaaa tggttgcgcc gaacggttct attggcttcc gctggggcga gaagggcaaa
    35161 tggaatcttg agcagcgcga cggcaaaact ggcgaagaaa ccgagctgca actgagcctg
    35221 ctgggtagcc aggatgagat cgctgaggta ggcttcccgt actttggtgg cgacggcacg
    35281 gaacacttca acaaagtgga actggaaaac gtgctgctgc acaaactgcc ggtgaaacgc
    35341 ctgcaactgg ctgatggcag caccgccctg gtgaccaccg tttatgatct gacgctggca
    35401 aactacggtc tggaacgtgg cctgaacgac gttaactgtg caaccagcta tgacgatgtg
    35461 aaagcttata ccccggcctg ggccgagcag attaccggcg tttctcgcag ccagattatt
    35521 cgcatcgccc gtgaatttgc cgataacgct gataaaacgc acggtcgttc gatgattatc
    35581 gtcggtgcgg ggctgaacca ctggtatcac ctcgatatga actatcgtgg tctgatcaac
    35641 atgctgattt tctgcggctg tgtcggtcag agcgggggcg gctgggcgca ctatgtaggt
    35701 caggaaaaa
//
LOCUS       DADLTY010000018        35530 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.018, whole genome shotgun sequence.
ACCESSION   DADLTY010000018 DADLTY010000000
VERSION     DADLTY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.018"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..365
                     /locus_tag="JN334_000760"
     CDS             <1..365
                     /locus_tag="JN334_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit tau"
                     /protein_id="HAY7437009.1"
                     /translation="AAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRG
                     AQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQ
                     TLRRFFDAELDEESIRPI"
     gene            418..747
                     /locus_tag="JN334_000761"
     CDS             418..747
                     /locus_tag="JN334_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAY7437010.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            747..1352
                     /gene="recR"
                     /locus_tag="JN334_000762"
     CDS             747..1352
                     /gene="recR"
                     /locus_tag="JN334_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAY7437011.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            1462..3336
                     /gene="htpG"
                     /locus_tag="JN334_000763"
     CDS             1462..3336
                     /gene="htpG"
                     /locus_tag="JN334_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAY7437012.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            3517..4161
                     /gene="adk"
                     /locus_tag="JN334_000764"
     CDS             3517..4161
                     /gene="adk"
                     /locus_tag="JN334_000764"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAY7437013.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            4293..5255
                     /gene="hemH"
                     /locus_tag="JN334_000765"
     CDS             4293..5255
                     /gene="hemH"
                     /locus_tag="JN334_000765"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAY7437014.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(5252..6211)
                     /gene="aes"
                     /locus_tag="JN334_000766"
                     /pseudo
     CDS             complement(5252..6211)
                     /gene="aes"
                     /locus_tag="JN334_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            6363..7667
                     /locus_tag="JN334_000767"
     CDS             6363..7667
                     /locus_tag="JN334_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HAY7437015.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(7800..9476)
                     /locus_tag="JN334_000768"
     CDS             complement(7800..9476)
                     /locus_tag="JN334_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HAY7437016.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(9714..10934)
                     /locus_tag="JN334_000769"
     CDS             complement(9714..10934)
                     /locus_tag="JN334_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7437017.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            11152..12804
                     /locus_tag="JN334_000770"
     CDS             11152..12804
                     /locus_tag="JN334_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY7437018.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(12841..13320)
                     /gene="ybaK"
                     /locus_tag="JN334_000771"
     CDS             complement(12841..13320)
                     /gene="ybaK"
                     /locus_tag="JN334_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HAY7437019.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(13524..14318)
                     /locus_tag="JN334_000772"
     CDS             complement(13524..14318)
                     /locus_tag="JN334_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437020.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            14456..14797
                     /locus_tag="JN334_000773"
     CDS             14456..14797
                     /locus_tag="JN334_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY7437021.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(15012..17516)
                     /gene="copA"
                     /locus_tag="JN334_000774"
     CDS             complement(15012..17516)
                     /gene="copA"
                     /locus_tag="JN334_000774"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HAY7437022.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            17778..18710
                     /gene="glsA"
                     /locus_tag="JN334_000775"
     CDS             17778..18710
                     /gene="glsA"
                     /locus_tag="JN334_000775"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAY7437023.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            18713..20005
                     /locus_tag="JN334_000776"
     CDS             18713..20005
                     /locus_tag="JN334_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7437024.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            20130..20537
                     /locus_tag="JN334_000777"
     CDS             20130..20537
                     /locus_tag="JN334_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HAY7437025.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(20538..20996)
                     /locus_tag="JN334_000778"
     CDS             complement(20538..20996)
                     /locus_tag="JN334_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAY7437026.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(20993..21910)
                     /locus_tag="JN334_000779"
     CDS             complement(20993..21910)
                     /locus_tag="JN334_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAY7437027.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            22056..22733
                     /gene="fetA"
                     /locus_tag="JN334_000780"
     CDS             22056..22733
                     /gene="fetA"
                     /locus_tag="JN334_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HAY7437028.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            22720..23499
                     /gene="fetB"
                     /locus_tag="JN334_000781"
     CDS             22720..23499
                     /gene="fetB"
                     /locus_tag="JN334_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HAY7437029.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(23562..24416)
                     /gene="cnoX"
                     /locus_tag="JN334_000782"
     CDS             complement(23562..24416)
                     /gene="cnoX"
                     /locus_tag="JN334_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HAY7437030.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(24477..25286)
                     /gene="ybbO"
                     /locus_tag="JN334_000783"
     CDS             complement(24477..25286)
                     /gene="ybbO"
                     /locus_tag="JN334_000783"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY7437031.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(25276..25898)
                     /gene="tesA"
                     /locus_tag="JN334_000784"
                     /pseudo
     CDS             complement(25276..25898)
                     /gene="tesA"
                     /locus_tag="JN334_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            25869..26555
                     /locus_tag="JN334_000785"
     CDS             25869..26555
                     /locus_tag="JN334_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7437032.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            26552..28831
                     /locus_tag="JN334_000786"
     CDS             26552..28831
                     /locus_tag="JN334_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7437033.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGGLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCDVRHAWVCFR
                     PGGRNWCGNGTGSVAGESV"
     gene            28809..28967
                     /locus_tag="JN334_000787"
     CDS             28809..28967
                     /locus_tag="JN334_000787"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7437034.1"
                     /translation="MLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGK
                     ALFRQFVG"
     gene            complement(29680..30774)
                     /gene="mnmH"
                     /locus_tag="JN334_000788"
     CDS             complement(29680..30774)
                     /gene="mnmH"
                     /locus_tag="JN334_000788"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAY7437035.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(30843..31769)
                     /gene="allS"
                     /locus_tag="JN334_000789"
     CDS             complement(30843..31769)
                     /gene="allS"
                     /locus_tag="JN334_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HAY7437036.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            31999..32481
                     /gene="allA"
                     /locus_tag="JN334_000790"
     CDS             31999..32481
                     /gene="allA"
                     /locus_tag="JN334_000790"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HAY7437037.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            32559..33374
                     /gene="allR"
                     /locus_tag="JN334_000791"
     CDS             32559..33374
                     /gene="allR"
                     /locus_tag="JN334_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HAY7437038.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            33464..35245
                     /gene="gcl"
                     /locus_tag="JN334_000792"
     CDS             33464..35245
                     /gene="gcl"
                     /locus_tag="JN334_000792"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HAY7437039.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            35258..>35530
                     /locus_tag="JN334_000793"
     CDS             35258..>35530
                     /locus_tag="JN334_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HAY7437040.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT         8479 a         9170 c         9510 g         8371 t
ORIGIN      
        1 gggcggcaca ggtgagccaa ctttcgctac caaaactggt cgaacaggtg gcgttaaatg
       61 cctggaaaga ggagagcgac aacgcagtat gtctgcattt gcgctcctct cagcggcatt
      121 tgaacaaccg cggtgcacag caaaaactgg ctgaagcgtt gagcatgtta aaaggttcaa
      181 cggttgaact gactatcgtt gaagatgata atcccgcggt gcgtacgccg ctggagtggc
      241 gtcaggcgat atacgaagaa aaacttgcgc aggcgcgcga gtccattatt gcggataata
      301 atattcagac cctgcgtcgg tttttcgatg cggagctgga tgaagaaagt atccgcccca
      361 tttgatcgta agcacagctt acgttcgtca tccttaacgt gattgagaga gaaacctatg
      421 tttggtaaag gcggtctggg taacctgatg aagcaagccc agcagatgca agaaaaaatg
      481 cagaaaatgc aggaagagat cgcgcagctg gaagttaccg gcgaatctgg cgcaggtctg
      541 gtaaaagtga ccatcaacgg tgcacacaac tgccgtcgcg tagagatcga cccgagcctg
      601 ctggaagacg acaaagagat gctggaagac ctggtggctg cagcattcaa cgacgcagca
      661 cgtcgtattg aagaaacgca gaaagaaaaa atggcctctg tatcctccgg aatgcagctg
      721 ccgcctggct ttaagatgcc gttctgatgc aaaccagccc gctgttaaca cagcttatgg
      781 aagcactgcg ctgtctgccg ggcgttggcc cgaagtcggc gcagcgtatg gcgttcacgc
      841 tgcttcagcg cgatcgtagc ggcgggatgc gtctggcgca ggcgctcacc cgggcgatgt
      901 cggaaatcgg ccactgcgcc gattgccgca ctttcaccga acaggaagtc tgtaacatct
      961 gttcgaatcc gcgtcgtcag gaaaacggtc aaatctgtgt ggtggagagt ccggcggaca
     1021 tctacgccat tgagcagacg gggcagtttt ctggtcgtta ttttgtgttg atggggcatc
     1081 tgtcaccgct ggacggcatc ggtccggatg atatcgggct tgatcgtctg gaacagcgtc
     1141 tggcagagga aaaaatcact gaagtgatcc tcgccaccaa tcccacggtt gaaggtgaag
     1201 ctaccgctaa ctacattgcc gagctttgcg cgcaatatga cgtggaagcc agccgaatcg
     1261 ctcatggcgt accggttggc ggcgaactgg aaatggtcga tggcaccacg ctgtcacact
     1321 ccctcgccgg gcgtcataag attcgttttt aagcaaacga gagcaggagc acctgctctc
     1381 gcttgaaatt attctccctt gtccccatct ctccctcatc ctgtttttaa ccttaaaatg
     1441 gcattattga ggtagaccta catgaaagga caagaaactc gtggttttca gtcagaagtg
     1501 aaacagcttc tgcacctgat gatccattct ctctattcca ataaagaaat cttcctgcgt
     1561 gagcttatct ctaacgcctc cgatgcggcg gacaagctgc gtttccgtgc gctatctaac
     1621 ccggacctgt acgaaggtga tggcgaactg cgcgttcgtg tctctttcga taaagacaag
     1681 cgcacgctga ccatctccga taacggcgtg gggatgaccc gcgacgaagt gattgaccat
     1741 ctggggacta tcgctaaatc cggtaccaaa tcattcctcg aatccctggg ttctgaccaa
     1801 gcgaaagaca gccagctgat cggtcagttt ggtgttggtt tctactctgc gtttatcgtg
     1861 gccgacaaag tgaccgtgcg tactcgcgcg gcaggcgaaa aaccagaaaa cggcgtcttc
     1921 tgggaatcgg ctggcgaagg tgaatatacc gttgccgata tcaccaaaga agatcgtggt
     1981 actgaaatca ccctgcatct gcgtgaaggc gaagacgagt tcctcgatga ctggcgcgtg
     2041 cgttccatca tcagcaaata ctccgaccat atcgcgctgc cggtagagat cgaaaaacgc
     2101 gaagagaaag atggcgaaac cgttatctcc tgggcaaaaa tcaacaaagc gcaggcgctg
     2161 tggactcgta acaagtcgga aatcaccgat gaagagtata aagagttcta caaacacatc
     2221 gcccacgact ttaacgatcc gctgacctgg agccacaacc gcgttgaagg taagcaggag
     2281 tacaccagcc tgctgtacat cccgtcccag gctccgtggg atatgtggaa ccgtgatcat
     2341 aaacacggcc tgaaactgta cgttcaacgc gtgtttatca tggacgacgc tgaacagttc
     2401 atgccgaact atctgcgctt cgtgcgtggt ctgattgact ccagcgatct gccgctgaac
     2461 gtttcccgtg aaatcctcca ggacagcacg gtaacgcgta acctgcgcaa tgcgctgacc
     2521 aagcgtgtgc tgcaaatgct ggaaaaactg gcgaaagacg acgcggaaaa ataccagacc
     2581 ttctggcaac agtttggcct ggtactgaaa gaaggtccgg cggaagattt cgctaaccag
     2641 gaagcgatcg ccaaactgct gcgttttgct tctacttata ccgattcttc tgcgcagacc
     2701 gtgtctctgg aagactacgt ttcccgcatg aaagaagggc aggagaaaat ctactacatc
     2761 actgcagaca gctatgcggc agcgaagagc agcccgcacc tggaactgct gcgtaagaaa
     2821 ggcatcgaag ttctgctgct ttccgaccgc atcgatgagt ggatgatgaa ctatctgact
     2881 gagttcgacg gtaaaccgtt ccagtcggtg tctaaagttg acgagtcgct ggaaaaactg
     2941 gctgacgaag ttgatgagag cgcgaaagaa gcggagaaag cactgactcc gttcatcgac
     3001 cgcgtgaaag ctctgctcgg cgagcgcgtg aaagatgtcc gtctgactca ccgtctgacc
     3061 gatacgccag cgatcgtttc gaccgacgcg gacgaaatga gcactcagat ggcgaaactg
     3121 ttcgctgcgg cgggccagaa agtgccagaa gtgaaataca tcttcgaact gaacccggat
     3181 cacgtactgg tgaaacgtgc ggcagatact gaagatgaag ccaagttcag cgagtgggta
     3241 gaactgctgc tggatcaggc gctgctggca gaacgcggca cgctggaaga tccgaacctg
     3301 tttattcgtc gtatgaatca gctgctggtt tcctgatgtc atgccggatg acctttgtgt
     3361 catccggcat ttttcttttc atcatctgta ctttccgcaa attatctcgc cattaaccgt
     3421 ttcagcctca ggtgcctttc ttgaggcaat cgcctgttgg tggtatcgtt tatcgctttt
     3481 tcaaaaaaat cgacacattt taaggggatt ttcgcaatgc gtatcattct gcttggcgct
     3541 ccgggcgcgg ggaaagggac tcaggctcag ttcatcatgg agaaatatgg tattccgcaa
     3601 atctccactg gcgatatgct gcgtgctgcg gtcaaatctg gctccgagct gggtaaacaa
     3661 gcaaaagaca ttatggatgc tggcaaactg gtcaccgacg aactggtgat cgcgctggtt
     3721 aaagagcgca ttgctcagga agactgccgt aatggtttcc tgttggacgg cttcccgcgt
     3781 accattccgc aggcagacgc gatgaaagaa gcgggcatca atgttgatta cgttctggaa
     3841 ttcgacgtac cggacgaact gattgttgat cgtatcgtag gccgccgcgt tcatgcgccg
     3901 tctggtcgtg tttatcacgt taaattcaat ccgccgaaag tagaaggcaa agacgacgtt
     3961 accggtgaag aactgactac ccgtaaagac gatcaggaag aaaccgtgcg taaacgtctg
     4021 gttgaatacc atcagatgac tgcaccgctg atcggctact actccaaaga agcggaagcg
     4081 ggtaacacca aatacgcgaa agttgacggt accaagcagg ttgctgaagt tcgcgctgat
     4141 ctggaaaaaa tcctcggcta atcaagccgc agcagatctg ctttcgggca ggtctgctta
     4201 tccacctcag gcaatacctc tcacagcaat tagttcttct tcctcacttt tccgctacaa
     4261 ttatcaacaa gttgaatcga taagaggcgg taatgcgtca gactaaaacc ggtatcctgc
     4321 tggcaaacct gggtacgccc gatgccccca cacctgaagc ggtaaaacgc tatctgaaac
     4381 aatttttaag cgacagacgc gtggttgata cctcacggtt gttatggtgg ccgttgctgc
     4441 gcggcgtgat tttgccgctg cgctcgccgc gtgtggcgaa gctgtacgcc tctgtctgga
     4501 tggaagacgg ctcgccgctg atggtttaca gccgccagca acagcaggcg ttggcacaac
     4561 gtttaccgga tacgccagtg gcgctgggca tgagctacgg ctcgccatca ctggaaagct
     4621 ccgtcgatga actcctggca gagcatgtag atcatattgt ggtgctgccg ctttatccgc
     4681 aattctcctg ttctacggtc ggtgcggtat gggatgaact ggcacgcatt ctggcgcgca
     4741 aacgtagcat tccggggata tcgtttattc gtgattacgc cgataaccac gattacatta
     4801 atgcactggc gaacagcgta cgcgcttctt ttgccaaaca tggcgaaccg gatctgctgc
     4861 tgctctctta tcatggcatt ccccagcgtt atgcagatga aggcgatgat tacccgcaac
     4921 gttgccgcac aacgactcgc gaactggctt ccgcactggg gatggtaccg gaaaaagtga
     4981 tgatgacctt tcagtcgcgc tttggccggg aaccctggct gatgccttat accgacgaaa
     5041 cgctgaaaat gctcggagaa aaaggcgtag gtcatataca ggtgatgtgc ccgggctttg
     5101 ctgcggattg tctggagacg ctggaagaga ttgccgagca aaaccgtgag gtcttcctcg
     5161 gtgcgggcgg gaaaaaatat gaatatattc cagcgcttaa tgccacgccg gaacatatcg
     5221 aaatgatggc taatcttgtt gccgcgtatc gctaaagctg agcggtaaag aactgagcgc
     5281 cgtcgcgaag agcctcgtcg gcggttttca tcatccgtga ataatgcaaa aaggcatgca
     5341 gcgtacctgg gtagagtttg aactcacagg gctgctgatg cgccgctaac gtctagtaaa
     5401 gcagacggct gtcatccagc agcggatcga actccgcccc ggcaataaaa cagggcggaa
     5461 cttcgcgagt gagatcatta ttaaacagac agtaatacgg tgactcgcgg tccgcgtcgt
     5521 tgcttaaata cgcctcttca tacatctgca aatcctgttg cgttaagcca tcccagacac
     5581 cgcccaacag acgacgagtc acggaatccc gtaatccgta aagcccatac cacagcaaaa
     5641 cacccgcaat tttaccgcaa tcgatctgtt tatcacgcaa ccacaacgca ctggcgagcg
     5701 ccagcatggc acctgcggaa tcaccggcaa agccaatgcg ggacatattg atttgataat
     5761 cctccgcctg ctggtggaaa taacaacaag cagccacaat ttcctctatc gcttgtggaa
     5821 aacgcgcttc aggtgaaagg gtgtaattaa taccaatcac cgtacattgg ctgtagcttg
     5881 ccagcaggcg catgatgcga tcgtgggtat cgagattgcc gagaataaaa ccgcctccat
     5941 gcaaataaaa tagcgtcgcc gggctatctg gctgcggaca aaagagacgt gtttccacct
     6001 gcccatattt tgttggaacc atgtaagctc tggttgccat ttctggagcg cccgcattcc
     6061 agaatcggcg ctcaagcgtg taatactgtc gttgctcagc aatcgttccc gttgcgggcc
     6121 aggacggtaa atccggctga agagtattca caacggtctt catttcagca gaaataaggt
     6181 ccagaacagg tagtttgttt tccggcttca tacaaaaact cctttcaaat tacgtcattg
     6241 taaggaacca ctgccatgaa aatgcgatcc cgcctgctga tattgaaact ggctgcgtct
     6301 cgcgcgctcc cgtcagattg tgttaacatt cgccgctcag ttaaccaccc gtaaaaacaa
     6361 ccatgaaatt tcccggtaaa cgtaaatcca aacattactt ccctgtaaat gcacgcgatc
     6421 cgctgcttca gcaattccag ccagaaaacg aaaccagcgc cgcctgggtc gtaggtattg
     6481 accagacgct ggtcgatatt gaagcgaaag tggatgatga attcattgag cgttatggat
     6541 taagcgccgg gcattcactg gtgattgagg atgacgtagc cgaagtgctt tatcaggagc
     6601 taaaacagaa aaacctgatt acccatcagt ttgcgggtgg caccattggt aacaccatgc
     6661 acaactactc ggtgctcgcg gacgaccgtt cggtgctgct gggcgtcatg tgcagcaata
     6721 ttgaaattgg cagttatgcc tatcgttacc tgtgtaacac ttccagccgt accgatctta
     6781 actatctaca aggcgtggat ggtccgattg gtcgttgctt tacgctgatt ggcgagtccg
     6841 gggaacgtac ctttgctatc agtccaggcc acatgaacca gctgcgggct gaaagcattc
     6901 cggaagatgt gattgccgga gcctcggcac tggttctcac ctcatatctg gtgcgttgca
     6961 agccgggtga acccatgccg gaagcaacca tgaaagccat tgagtacgcg aagaaatata
     7021 acgtaccggt ggtgctgacg ctgggcacca agtttgtcat tgccgagaat ccgcagtggt
     7081 ggcagcaatt cctcaaagac cacgtttcta tccttgcgat gaacgaagat gaagccgaag
     7141 cgttgaccgg agaaagcgat ccgttgttgg catctgacaa ggcgctggac tgggtagagc
     7201 tggtgctgtg caccgccggg ccaatcggct tgtatatggc gggctttacc gaagacgaag
     7261 cgaaacgtaa aacccagcat ccgctgctgc cgggcgctat agcggaattc aaccagtatg
     7321 agtttagccg cgccatgcgc cacaaggatt gccagaatcc gctgcgtgtc tattcgcaca
     7381 ttgcgccgta catgggcggg ccggaaaaaa tcatgaacac taatggagcg ggggatggcg
     7441 cattggcagc gttgctgcat gacattaccg ccaacagcta ccatcgtagc aacgtaccaa
     7501 actccagcaa acataaattc acctggttaa cttattcatc gttagcgcag gtgtgtaaat
     7561 atgctaaccg tgtgagctat caggtactga accagcattc acctcgttta acgcgcggct
     7621 tgccggagcg tgaagacagc caggaagagt cttactggga tcgttaagtt atcgtcggtt
     7681 cgtaggccag ataaggcgtt cacgccgcat ctggcatttg gctctcgatg cctgatgcga
     7741 cgctggcgcg tcttatcatg cctacatatt tttcatattt tacatccggc aaccaccgtt
     7801 taccccgtca ccacttcacc cgccggtggc gtttccagca gttccagcat agtacgggca
     7861 atttcacgtt cacccatcac tacctgattc gcgccacgtt cggtgatata cgtcacttca
     7921 tcgtcataat gggcgcgggc gataatctca atatccgggt ttttcgcgcg ggcagacgcc
     7981 acaatctcac ccgcttcata accgttgggg atcgtcagga tcagccattt tgcacattcc
     8041 agatgtgcca gttgcataat ttcttcgttc gctgcattgc ccaatacagc gcggacccca
     8101 cgctctcgca gctcatcaac acgggttcgt gacgtctcaa tcaccaccag cggaatatca
     8161 gaggcgagca atttctcccc cagcaggctg cctacacgac cgtaacccac caatagcgca
     8221 tggttgcaaa tatccactgg gatctgtttc tcttcttcga ttgcctcttc cagcgtctgc
     8281 tcttccagcg tttcggtctt cgccagatat ttctccagta gtgcgaacag taccgggttg
     8341 agcataatcg acaggatcgc ccctgccagt accaggtttt gtccggcctg cggcagtaaa
     8401 ttcaatgcca ttcccagtcc cgccaggata aacgcgaact caccaatctg cgccaggctg
     8461 gcggcgatgg ttaatgccgt acgttgggag tgaccaaaca gacggacaag gaacagggcc
     8521 gcgaccgatt taccaaacag aataattgcc agcgtcgcca gcactgccag cggttgctga
     8581 atcagaatta acggatcaaa caacatcccg actgagacaa aaaacagcac cgcaaacgcg
     8641 tcgcgcaatg gcaacgtatc gtgggcggca cggtgactca attcagactc gttcagtacc
     8701 atcccggcaa agaacgcacc gagtgcaaag gagacatcaa acagctctac cgcaccaaag
     8761 gcaatcccta acgccagcgc cagcactgac agggtaaaca gctcgcgaga accggttgcc
     8821 gcgctgcgtg ccataatcca cggcaccaga cggcgaccta ccagcatcat aatggcgata
     8881 aatgcgatca ccttgccgat ggtgatcccc atatcgactg caagagtggc aaagcccaca
     8941 tcgccctgtt ccatcattcc tgccactgcg ggcagcaaca ccagcgtcag aaccatcacc
     9001 aggtcttcca caatcaacca accgatggcg atttgcccac gctgactgtc tattaattgc
     9061 cgttcttcaa gtgcgcgcag taacaccacg gtactggcgg tggaaagaca taagccgaac
     9121 acgataccgg tcattaacga ccagcccagc acggcggaga gcgccatacc cagcagcgtc
     9181 gccacggcta tctgggcgat cgcgccggga atggcgatgg actttaccgc catcaaatcc
     9241 ttgagcgaaa aatgcaaacc gacgccgaac atcagcaaaa taacgcccag ttcagccagt
     9301 tccggcgcaa gcttggtatc ggcaacaaag cccggagtga atggtcctgc cagcacaccc
     9361 gctaacagat atcccaccag aggagaaata cgtagtttat tggccagcat gccgaggata
     9421 aaggcgagca caaggccgcc aacaatggtg gtgataagcg gggtggcgtg atgcattccg
     9481 tctccttttc ctggtggtta ttgtccattt ttggctggga aaaccaaaat tacaggtaat
     9541 agtttatgac aatttcattg atgatgttta tgaataattg ttgaattttg cagaaaaatg
     9601 gaattagctg caaaaaaagc acggttcgga aaacagaagg cggtttaaca gaggaaaggc
     9661 ttatggcgac tgtaaaacga tgcagccaaa gtttgccttt ggctgcaatg aaatcagtct
     9721 ttatgccggt tatcaggcag gaatatggtc aacatcccca atagtggcag gaaagcacag
     9781 attttataga ctaactcgat gctggtgtga tcggcgataa gccccagaac tgccgctccc
     9841 agacctccca tgccgaaagc aaaaccgaaa aagagtccag aaaccatacc gatacgtccc
     9901 ggaagtagct cctgagcgta gaccagaatg gcagagaatg ccgaagcgag gataaatcca
     9961 ataatcaccg ttaaaacccc cgtccagtgc aggcgggcgt agggtaaaat cagcgtaaac
    10021 ggcgcaacgc cgaggataga gccccaaatc acatatttcc gtccaatttt atcccctaca
    10081 ggcccgccga tcaccgtacc tgccgcaacg gcaaacagga aggcaaacag atgaagctgg
    10141 gcattctgga tagataatcc gaatttttgc atcagataaa aggtgtaata gctgctgatg
    10201 ctcgccatat agaaatattt cgagaaaatg aggattaaca gaatgctgac tgccagtacc
    10261 actttgttgc gcggcagtgg attgattatc gtcgctttgg gttttccttt attcattcgg
    10321 tgctgtgccg agtaccaacg gctaatttgc gccaacacca cgatcgccag cagtgccgca
    10381 agcacaaacc aggcaacgtt gcctttgcca taaggcgcga taatcaccgc cgccagcaag
    10441 ggtcccaggg aactgccaaa gttgccgccg acctgaaaga tagattgcgc caggccatgc
    10501 cgcccgccgg aagccatacg ggctacgcga gaagattccg gatgaaagac cgatgaaccg
    10561 gtaccgacca gcgccgctgc cagcagaact gcgccaaaac tgcccgccag cgcaagcagc
    10621 accagaccac ttaaggtaaa gcacatgcca attggcaacg accacggcat cggatattta
    10681 tcggtccagt agccgaccac tggttgcagt agcgaagagg cgagctggaa ggtgagggtt
    10741 atcatgccaa tctgcataaa tgtcagagaa aattctgact gaagcagcgg ataaatcgcc
    10801 agaatcagcg attggatcat gtcgttcagc agatgtgaga ggctgatagc acctaaaata
    10861 ccaaacgatg ttcgggcctt ggtcgttgac gcagccgcgc ccgccacagg ctgggtttgt
    10921 tcactcattg ccataggaaa gtcacttttt cagggttgcg atgtaaagaa tgatcttatt
    10981 tgtgattatt acccgactaa catacctgta tgcgtcgtct gaaggaagtc tcaacgccga
    11041 atacagaatt tctaatctgg atgcagattt atcttcaccg gacgcagact tgtctatgat
    11101 gttgcgtcat actatttttc aacacgttga aatcaggtca gggagagaag tatgaaatta
    11161 ttgcagcggg gcgtggcgtt agcgctgtta accacattta cactggcgag tgaaactgct
    11221 ctggcgtatg agcaggataa aacctacaaa attacagttc tgcataccaa tgatcatcat
    11281 gggcattttt ggcgcaatga atatggcgaa tatggtctgg cggcgcaaaa aacgctggtg
    11341 gatggtatcc gcaaagaggt tgcggctgaa ggcggtagcg tgctgctact ttccggtggc
    11401 gacattaaca ctggcgtgcc cgagtctgac ttacaggatg ccgaacctga ttttcgcggt
    11461 atgaatctgg tgggctatga cgcgatggcg atcggtaatc atgaatttga taatccgctc
    11521 accgtattac gccagcagga aaagtgggcc aagttcccgt tgctttccgc gaatatctac
    11581 cagaaaagta ctggcgagcg cctgtttaaa ccgtgggcgc tgtttaagcg tcaggatctg
    11641 aaaattgccg ttattgggct gacaaccgat gacacagcaa aaattggtaa cctggaatac
    11701 ttcactgata tcgaatttcg taagcccgcc gatgaagcga agctggtgat tcaggagctg
    11761 caacagacag aaaagccaga cattattatc gcggcgaccc atatggggca ttacgataat
    11821 ggtgagcacg gctctaacgc accgggcgat gtggagatgg cacgcgcgct gcctgccgga
    11881 tcgctggcga tgatcgtcgg tggtcactcg caagatccgg tctgcatggc ggcagaaaac
    11941 aaaaaacagg tcgattacgt gccgggtacg ccatgcaaac cagatcaaca aaacggcatc
    12001 tggattgtgc aggcgcatga gtggggcaaa tacgtgggac gggctgattt tgagtttcgt
    12061 aatggcgaaa tgaaaatggt taactaccag ctgattccgg tgaacctgaa gaagaaagtg
    12121 acctgggaag acgggaaaag cgagcgcgtg ctttacactc ctgaaatcgc tgaaaaccag
    12181 caaatgatct cgctgttatc accgttccag aacaaaggca aagcgcagct ggaagtgaaa
    12241 ataggcaaaa ccaatggtcg tctggaaggc gatcgtgaca aagtgcgttt tgtacagacc
    12301 aatatggggc ggttgattct ggcagcccaa atggatcgca ctggtgccga ctttgcggtg
    12361 atgagcggag gcggaattcg tgattctatc gaagcaggcg atatcagcta taaaaacgtg
    12421 ctgaaagtgc agccattcgg caatgtggtg gtgtatgccg acatgaccgg taaagaggtg
    12481 attgattacc tgaccgccgt cgcgcagatg aagccagatt caggtgccta cccgcaattt
    12541 gccaacgtta gctttgtggc gaaagacggc aaactgaacg accttaaaat caaaggcgaa
    12601 ccggtcgatc cggcgaaaac ttaccgtatg gcgacattaa acttcaatgc caccggcggt
    12661 gatggatatc cgcgccttga taacaaaccg ggctatgtga ataccggctt tattgatgcc
    12721 gaagtgctga aagcgtatat ccagaaaagc tcgccgctgg atgtgagtgt ttatgaaccg
    12781 aaaggtgagg tgagctggca gtaatccgaa agtgccggat gtttgcatcc ggcacaatgc
    12841 ttaatcgcgg cgggcgatat cagcaaattt ggcatcgagg atctttgcca gatcgcctgc
    12901 cgccagttcg atatccagtc cgcgcttgcc gccggaaaca taaatagtgg caaattcttg
    12961 tgcgggggcg tcgataatcg ttggcagacg ttttttctgc cccagtgggc taatcccccc
    13021 aaccaggtat cccgtcgaac gctgcgcgac catcggatcg gccatctcaa ctttcttggc
    13081 acccagcgct tttgctactt ttttaagatc cagttgacct gcgaccggcg taacggccac
    13141 ggcaaggtgt ttcatatcac cgttcactgc caccagcagc gttttgtaga cctgatccgg
    13201 attcaaacct aattttttga cgacttcatc gccaaaattg gtttcagccg gatcgtgctc
    13261 gtaggtatgg atttgaaacg aaatcttgtt tttttcgagt aatttaactg cgggtgtcat
    13321 ggtaattttc ctgccttaaa caaaaaaacg aagcaagcat acgcctgatg gcggtccaaa
    13381 aaaaagagtc atcttgccta agagtattgg caggatggtg agattgagcg acaatcgagt
    13441 tacaccgtcg cttaaagtga cggcataata ataaaaaaat gaaattcctc tttgacgggc
    13501 caatagcgat attggccatt tttttagcgc aacatttgcg gcaaattccc ttctccatac
    13561 aggtgtagtg caccgaccgc gaccacatat cgccccggcg gcatggcgcg taatttatcc
    13621 cgccaggcga gatttcgctg atgcatcagc acatcgtaca gcgactgact gaacgtattg
    13681 ggcagcgtta tatcattatt ttgcggcggt gcattcagcc accagctcat catttgttgc
    13741 agcaaccgtg cgttggtatg ccagtgggtc agcgtatcgt ccagcagcgc cagtcctttg
    13801 tcagggagct ggagcaacat ggcaatctgg ttttcagccc cttccagttc aatcacgggt
    13861 ttatgttgtt gcttcgccgc ctgcaatagc tggtaatcga taccgtattc tgcccgcagc
    13921 cccagttttt gtgcctgcgt cgcctgaaga accatcgcga tttgccacag cggttgggta
    13981 gaaaagagtg agggagaaat gcccatctcc tggctaatgt gctgcaggtt ttggagttgc
    14041 tcctcgctaa tgcgctcttc cagcgcctcg caggcaggca aattagcaaa aggcgtatcg
    14101 ctggtggaaa catccgcctc gacgatcagc gcatcggcgt ttttgagctt tttgagcaaa
    14161 cgggtgggca ggggagccat atcgtggcta cccatatgaa tactgccaat cagatgaaaa
    14221 tggcgattgc cggggagggt gatatcgatg gcaggccagg tgtaatgatt accgcgcagc
    14281 gcggcccaaa gcgtttttac ccggtacaac agatccatac gacctccctt tgtgaaatat
    14341 catgctagcg cgcggtgagg gatggcgcaa atggagttgg ggacttgttt aatgtgtttg
    14401 tatgattcag tatgttcttg catcgctatt cacagggaag caacagttaa aaaccatgaa
    14461 acaggcaaca agaaaaccga cgacacccgg agatattctt ctctatgaat atctggaacc
    14521 gctcgatttg aaaatcaatg agttagcaga gttgctgcat gttcatcgta atcgcgtcag
    14581 tgcactgatc aataacaatc gtaaactcac cactgagatg gcattccgtc tggcgaaagt
    14641 ttttgatacc acagtcgatt ttaggctaaa cctccaggcg gcagttgatc tttgggaagt
    14701 tgaaaacaac atgcgcaccc aggaagaatt gggacggatt gaaacagtgg ctgaatattt
    14761 ggcacgccgt gaagagcgtg caaaaaaggt cgcgtaaggc caaaacatcg cttcatctcg
    14821 taggccggat aaggcgttca cgccgcatcc ggcatccgag caccattgcc tgatgcgacg
    14881 cttacgcgtc ttatcaggcc tacaaacctg tgcctgaacc gtaggccgga taaggcgttt
    14941 acgccgcatc cggcagtcat gcgtcgatgc caaatgcgcc gccctaaagc agcgcatccg
    15001 caatgacgta cttattcctt cggtttaaac cgcagcaacc ggttggcgtt acttactacg
    15061 gtaattgacg agagcgccat tgccgctccg gcaactaccg ggttaagcag tgttccagta
    15121 aacggccaca aaataccggc ggcgaccgga ataccgatgc tgttgtagat aaaggcaccg
    15181 agcaggttct gcttcatgtt gcgcagcgtt gcgcgggaaa tcgcgagcgc atccgcaacg
    15241 cccatcaggc tatggcgcat cagggtaatc gccgcggttt caatggcaac atcactgccg
    15301 ccacccatcg caatgccgac atccgcctgg gccagcgctg gcgcgtcgtt aatgccgtcg
    15361 cccaccattg ccacctgacg tccttcactt tgcagacgtt tgatcgcttc ggctttaccg
    15421 tccggcagca ctccggcaat cacttcatca attccagctt ctttggcgat cgcattggcg
    15481 gtggttgggt tatccccggt caacatcacc agacgatatc ccgctttatg caggcgttgc
    15541 agcgccgcca cgctatcact acgcaacgga tcgcgtacag ccagcagggc tactgctttc
    15601 ccgtcaatcg ccagcagcac aggcgttgct ccttgcgatg cctgagctga aatatcggct
    15661 tcaatcgctt tagtatcgac ttgctgatca tttaacaacg cctgattacc cagcaataac
    15721 gcatgacctt ctgcttcacc gctcacgccc agcccgcgca atgtgcggaa accgttgacc
    15781 tgcggtagct gcatatcgcc tgctttatca aggatcgctc gtgccaatgg atggctggaa
    15841 ccttgctcca gcgccgccgc cagacgcagc gcctgcgctt cgtcaacatc agcaaatgtt
    15901 ttcactgcga caacctgcgg cttcccttcg gtcagcgtcc cggttttatc gaacacgacg
    15961 gtatcgagtg tactggcgcg ttgcagcgcg tcggcgtccc gcaccagcac gccaaactca
    16021 gccgcccgcc cgacgccgga aataatcgac atcggcgtcg ccagtcccag cgcacacggg
    16081 caggcgataa tcagcaccgt agtggcaatc accagggtat agacaatctg cggtgccgga
    16141 ccaaagaaat accagattgc cgcactgaca agcgcaataa ccactactac cggcacaaat
    16201 atggctgaga ttttatccgc cagctgaccg atttctggct tgctgctctg ggcctggcgc
    16261 accatgcgaa tgatccttga tagcgtggta tggctgccaa ccgcactggc acgaaacagc
    16321 acactgccgt cctgtaccac tgtcccggca tggacgctat cgccttcgcc tttttgctgc
    16381 gggattggtt cgcccgtcag catcgcttca tccagccatg cttcgccctg ggtaatctcg
    16441 ccatctaccg gcacgcgatc gccggtcgtc aggcgcagca acatacctgg ctgcacttct
    16501 gccagaggca cgcttttttc accttcgtca gtaaccaggc gcgccgtcgg tggggttaaa
    16561 tcgagtaatt tctccagtgc cttagaagaa cgctgacgtg cgcgtgcttc cagcatatgg
    16621 ccgagattga tcagaccgat aatcatcgca ctggcttcat agtaaagatg tcgcgcttcc
    16681 atcgggaacc actgcggcca caggttgacg ctcatcgaat agagccacgc cacgcccgtt
    16741 cccagcgcca ccagcgtatc catcgtcgct gcaccgttca gcaggctttt ccatgcactg
    16801 cggtaaaaat ggccgctggc gaaaaccatc accgccaggg ttatcaggcc gataaccaac
    16861 cacaggctgc ggttgtcggc ggtgactatc atgttatcgc cgatcatccc ccagaccatc
    16921 accgggatac ccaccgccag tgcgacaatt gcctgccagc ggaagcgctt catcgtagcg
    16981 acggcggttt cttgctggcg ttcgcggcgt ttcgcgtcat cttcaatcgc ttccgcgccg
    17041 tagcccgctt tttccaccgc ctgcactaaa tcttgtgggg aggcactgcc catcaccagc
    17101 gcagtacgct ccgccaggtt tacccgtgcc tgagtgacgc ccggtacgct ttgcagcgca
    17161 ttttgtacgc gggtgacaca gctggcgcag ctcatgccgc tcagcagcaa ctgctggctg
    17221 tcatcgtcat cggcggtcgc tgccggaagc gcctcagaaa ccgctgtcag tgcttccgac
    17281 gggattgatg actccgccag cggtttagcc tttgggtggc ttacagatgc gtcataaccc
    17341 gcttgtttga tggtttcgat cagctgttct gcactggcag tcccggtaac gtgcgcttca
    17401 gtgatagaca catccgcttg ttccacatcc gggcgctgtt caagactttc tttcacgcgt
    17461 ttaacgcagt gaccgcagga caggccgtcc aggttcaggt cgatagtttg tgacataaaa
    17521 cactccttta agacagtttt gactggctgt gataaaggtt aaaccttcca gcaaggggaa
    17581 ggtcaagaaa ttaataaacc aggcgggtaa aagtccgtaa agattaaaaa atcggctcga
    17641 tttgcatcag gattagacat ttatttcttt gttttcctgt agttaagttg cgggtgctaa
    17701 gttaaatcag gatgcctgaa aatcggcact ggggtgagga attacctccc gcatctataa
    17761 aaaggagtta acaaaagatg ttagatgcaa acaaattaca gcaggcagtg gatcaggctt
    17821 acacccaatt tcactcactt aacggcggac aaaatgccga ttacattccc tttctggcga
    17881 atgtaccagg tcaactggcg gcagtggcta tcgtgacctg cgatggcaac gtctatagtg
    17941 cgggtgacag tgattaccgc tttgcactgg aatccatctc taaagtctgt acgttagccc
    18001 ttgcgttaga agatgtcggc ccgcaggcgg tacaggacaa aattggcgct gacccgaccg
    18061 gattgccctt taactcagtt atcgccttag agttgcatgg cggcaaaccg ctttcgccac
    18121 tggtaaatgc tggcgctatt gccaccacca gcctgattaa cgctgaaaac gccgaacaac
    18181 gctggcagcg aattttacat atccaacagc aactggctgg tgagcaggta gcgctctctg
    18241 acgaagtcaa ccagtcggaa caaacaacca acttccataa tcgggccatt gcctggctgc
    18301 tgtactccgc cggatatctc tattgtgatg caatggaagc ctgtgacgtg tacacccgtc
    18361 agtgctctac gctcatcaat actgttgaac tggcaacgct tggcgcgacg ctggcggcgg
    18421 gtggtgtgaa tccgttgacg cataaacgcg ttcttcaggc cgacaacgtg ccgtacattc
    18481 tggccgaaat gatgatggaa gggctgtatg gtcgctccgg tgactgggcg tatcgcgttg
    18541 gtttaccggg caaaagcggt gtaggtggcg gtattctggc ggtcgtccct ggcgtgatgg
    18601 gaattgccgc gttctcacca ccgctggacg aagaaggcaa tagtgttcgc ggtcaaaaaa
    18661 tggtggcatc ggtcgctaag caactcggct ataacgtgtt taagggctga tcatgatgaa
    18721 cacggaaggt aataacggta acaaacccct cggtctatgg aacgtcgttt ccatcggtat
    18781 tggggcaatg gtgggggcgg ggatcttcgc gctgctgggg caggctgcgt tgctaatgga
    18841 agcctcgacc tggggcgcct ttgcttttgg cggtattgtg gctatgtttt ccggttatgc
    18901 ctatgcgcgt ctgggggcga gctatcccag caatggcggt attatcgact tttttcgtcg
    18961 cggattaggc aacggcgtct tttcgctagc gctctcgttg ctatacctgt tgacgctggc
    19021 ggtgagcatc gccatggttg cccgtgcttt tggcgcttat gccgtgcagt ttttgcatga
    19081 aggcagccag gaggagcacc ttattttgct ctacgcgttg gggatcattg cggtgatgac
    19141 gcttttcaac tccttaagca accatgcggt agggcggctg gaagtgatcc tcgtcggcat
    19201 taaaatgatg atcctgttat tgctgattat tgccggtgtc tggtcgctgc aaccggcgca
    19261 tatttccgtc tctgcgcccc ccagctccgg tgcgttcttc tcctgtattg ggataacctt
    19321 ccttgcctat gcgggctttg gcatgatggc gaacgcggcg gataaagtga aagatccgca
    19381 ggtcattatg ccacgggcgt ttctggtggc gattggcgtt accacgttgc tttatatctc
    19441 gctggcgctg gttttgctta gcgatgtatc ggcattagag ttagaaaaat atgctgatac
    19501 cgccgtagcg caggctgctt ctccgctgct cgggcatgtg ggttatgtga tcgtcgtcat
    19561 cggcgcttta ctggcgacgg cttcaggcat taacgcgaac ctgttcgccg tgtttaacat
    19621 catggacaac atgggcagcg aacgcgaact gccgaagcta atgaataaat ccctgtggcg
    19681 gcagagtacc tggggcaata tcatcgtcgt ggtgttgata atgctgatga cggcggcgct
    19741 aaatttagac tcactcgcca gcgttgccag cgccaccttt ttgatctgct atctggcggt
    19801 gtttgtggtg gcgatccgcc tgcgtcatga tattcacgcc tcgttgccga ttcttatcgt
    19861 tggtacgttg gtgatgttgt tggtgatcgt tggctttatc tacagcctgt ggtcccaggg
    19921 cagccgtgcg ttgatatgga ttattggtgc aatcttactc agccttattg tggcaatggt
    19981 catgaagcgt aataaaaccg tataacacct ctctgtgcgc agtacttcct gtattattgt
    20041 ggtggcggtc gatattcgca ctggcaaaaa aacgtgcttg aatatctgtt gaaacccttt
    20101 aacaaagcac aggaggcgtt gcgcgaacga tgaacatcag cgatgtagca aaaattaccg
    20161 gcctgaccag caaagccatt cgcttctatg aagagaaggg gctggtgacg ccgccgatgc
    20221 gcagcgaaaa cggttatcgc acctacacgc agcagcatct caacgaactg accttactgc
    20281 gccaggcacg gcaggtgggc tttaacctgg aagagagcgg cgagctggtg aatctgttta
    20341 acgacccgca gcggcacagc gccgacgtca aacggcgcac gctggagaag gtggcggaga
    20401 tcgaacgaca cattgaggag ctgcaatcca tgcgcgacca gctgctggca ctggcgaatg
    20461 cctgccctgg cgatgacagc gccgactgcc cgattatcga aaatctctcc ggctgctgtc
    20521 atcatcgggc agggtgatta agacgagacg gctcggatat gtagggttat cccttctatc
    20581 gcaatgactt caacatgcgt acctgcaccg agatcctcgc tggcgctgac aggccatgaa
    20641 ctgtcaccga cgcgcatatg accgcgcccg ttgaccagcg gagattccag cacaaaacgt
    20701 cggccaatca gctgctgccc gcgctggttt aaatgactat cgctgtgctt ttgttcgcgc
    20761 acccgccgcg acaaccattt ccaccacagc caggcggcga gcagcgtcag gatggcaaac
    20821 attacccctt gccactccca acccagcggc accagccaga ccaccaggcc ggtaatcacc
    20881 gctgccacgc cgctccacaa caaataacca tttccgccca gcatctcggc tgccagcagc
    20941 aacccgccga gactgagcca gaaaatatgt ggatgaacga ccattaactc catcatggct
    21001 gagtccgttt gttggcgctg tctttcacca gctcggcaat cccggcaatc gaccccatca
    21061 ggctgctggc ctctaatggc atcatcacta ctttgctgtt actggaggaa ccgatctgct
    21121 gtaacgcttc ggtgtatttt tgcgctacga agtagttcac cgcctgaata tcaccggagg
    21181 cgatagcttc agacaccatt ttggtggcgc gggcttctgc ttcggcggaa cgttcacgcg
    21241 cttcagcctg taaaaacgcc gactgacgtt cgccttccgc tttcaggatt tgcgactgtt
    21301 tttcaccttc ggctttgagg atttccgcct gacggatccc ttccgcttca agaatgtaag
    21361 cgcgtttggt acgttccgct ttcatctgcg cgttcattga agagataagc tctgccggtg
    21421 ggcgcacgtc gcgaatttca atacgggtga ctttaatccc ccacgggttg gtggcttcat
    21481 cgacaatacg cagcaggcgt gagttgatgc tgtcgcgctg agagagcatt tcgtcaagtt
    21541 ccattgaacc caacacggta cggatgttag tcatggtcag gttgatgatc gccagctcca
    21601 gattgctgac ttcataggca gcgcgtggcg cgtcaatcac ctgaataaaa cagactgcgt
    21661 cgatggtaac gttggcgtta tctttcgaga taacttcctg ggaagggata tcgagcactt
    21721 gctccatcat attgatcttg cgaccaatgc gatccataaa cggcaccacc agactgagcc
    21781 ccggctgtaa cgttttggta tagcgaccaa aacgttctac cgtccattga tagccctgcg
    21841 gcacgatttt gacgcccgcg ccgacaatga ccagcgcgac aaaaatgaga atcgggataa
    21901 agataagcat cggaaaaacc tcctgttgta ccgtccataa tcagcaaaat tgctgcttga
    21961 ttaaacaaat tatacctgat tactgaaaga gagttccccc ttattcctgc gaaggataaa
    22021 ctgtttttag taaaaatcag aaaaagggaa cagcgatgca ggaaaatagt cctttgcttc
    22081 agctacaaaa cgtaggatat ctggcgggtg atacgaagat tcttaataac atcaattttt
    22141 cgctgcgtgc tggcgaattt aagttaatta ccggtccttc tggttgtggc aaaagtacgc
    22201 tgctaaaaat agttgcttca ttgatcagcc caaccagcgg aacgttactg tttgaaggtg
    22261 aggatgtcag cacactaaaa ccagaaatct accgccagca agtctcttac tgcgcccaga
    22321 cgccgacgct gtttggcgat acggtatacg ataatctgat ctttccctgg cagatccgtg
    22381 accagcagcc tgacccggcc atttttctcg attttctcga acgcttcgct ttgccggaca
    22441 gcattttgac gaagaatatc gccgagctat ctggtggtga aaaacaacgc atctcattga
    22501 ttcgtaacct gcaatttatg ccgaaggttt tattgctgga tgaaataacc agtgcgctgg
    22561 atgaaagtaa taaacataac gtcaatgaga tgatccatcg ttatgtgcgc gagcaaaata
    22621 ttgccgtgct gtgggtgaca cacgataaag acgaaattaa tcatgcggat aaagtgatta
    22681 cactgcaacc gcatgccgga gaaatgcagg aagcacgcta tgaactcgca taatattact
    22741 aacgaatcat tagcactggc attaatgctg gtggtggtgg caatcttaat tagccataaa
    22801 gaaaaactgg cgctggagaa agatattcta tggagcgtcg ggcgagcgat aattcagctg
    22861 attattgtcg gctatgtgct gaagtatatt ttcagcgtgg atgatgccag cctgacatta
    22921 ttgatggtgt tatttatctg ctttaatgcg gcgtggaacg cgcaaaaacg cagtaaatat
    22981 attgctaaag cttttatctc atcgtttatt gccattacgg tcggggcggg aattaccctg
    23041 gcggtgctga ttctctcagg ggcgattgaa tttatcccga tgcaggtgat ccctgtcgcc
    23101 gggatgattg ccggtaacgc catggtagcg gtggggttgt gttacaacaa tttagggcaa
    23161 cgggtcatta gcgaacagca acagattcag gagaaactga gtcttggcgc gacgccgaag
    23221 caggcttcag cgatattgat tcgcgacagt attcgcgcgg ctttaattcc gacggtcgat
    23281 tcagcaaaaa cggttggctt agtgagttta ccaggaatga tgtccgggct gatatttgcc
    23341 gggattgatc cggtgaaggc gattaaatat cagattatgg tgacctttat gctgctctca
    23401 accgccagct tgtcgaccat tattgcctgc tatttaacct atcgtaagtt ttataattcg
    23461 cgccaccagt tggtggtgac tcaattgaag aagaaatgat gcgatgccgg atgcgcagca
    23521 tcgcatccgg cgttgtggtt catgtgccga acaacggaat atcaatacaa caatgcatac
    23581 agctggcggc gatacttcga cgccagtgca tcacccgtac ccagcgcagc gaggatctcc
    23641 tggaacgttt tacgcgtctg accgtctgcg gcggtgagat ctttacgcag atgcccgaac
    23701 agcaactcca gcgcctcttc attgcgccca acctgatgca gttgcagcgc cagttgcgtc
    23761 gccagtgcgg catcttctgg attctccgcc acctgctgtt gcaactgttg aatttccggc
    23821 ttatcagccg cctgcttcag cagttcgatt tgcgctacca gcccctggta gcgggtgtcc
    23881 tgatcctgca acggaatggt tttcagcacc gcttccgcat cttcagaacg gttcagcgca
    23941 atcagcgttt ctgccagcag caggccgatc tccccgttct gattcgacaa ctgccaggcg
    24001 tctttcagca atggcagggc atcggtgtaa ttgccttcct gcatcagttg catcgcctgc
    24061 tgcgctttca gctcttcttc gcgcggcagc actttatcca gcagggcgcg gatcgcctct
    24121 tccggttgcg gcccctggaa gccatctacc ggttgcccgt tctggaacag atacacggtc
    24181 ggaatcgcgc gcagaccaaa ctgggcggca atcatctgct ccgcgtcgca gtccagcttc
    24241 gccagaataa attgcccgtt gtactgcgcc gcgaggcttt ccaggatcgg ggttaactgc
    24301 aaacagtgct ggctacgttc agaccaaaaa tagaacagca ccggagtggt catcgactgt
    24361 tcaagaacct gttgcaggtt agattcgtta atgttgacaa tattttctac ggacatggag
    24421 tcgctctctg ttgtcgatat tttctttgac atgggggctt aagcgcgcgc ttcaactcac
    24481 ccctgcaata ttttgtccat cacgcgccca ggcagcaggc gctcgagcac cattactgcc
    24541 caggtcacca gcgttaccgg atagcgcatc ttcggcttct cgctaataaa agcatggcgc
    24601 actttgtcga ccaccgcttc cggtcccaac gtaaagcggg cggcgatgcc gggattttcg
    24661 actggtttat cactttgcgt ctggttgacg ttgtcggtga agcgagtacg aatgggaccg
    24721 ggttcgatca ggctgacttt aattccgctg tggcgtagct ccatgcgcag cgcgtctgac
    24781 cacgcctcca gcgcatattt actggccgcg taagcgccac gacccggcgt ggagattaat
    24841 cccatcaccg atgatgtcat cacaatacgc ccttcaccgt gcggtaacat cgcgggtaac
    24901 aggcgcatgg tgagctggtg tgcgccgaaa aagttggcgg aaaactgctg ttccatctgc
    24961 gcacggctga tggtggaaag ggggccatac atgccgaatc cggcattgtt aaagatccca
    25021 tacagacaat tatcggtcag ggcgatcacc tcgtctgctg cgcgattaac actttctggt
    25081 gaatccagat cgatcaacac gccggtaaat cccatgttgt tcatacgctc aacatcattc
    25141 ggttttcggc aacctgccag cacatgaaaa ccctggcgtt ttaattcgag cgcgctttcc
    25201 aggccaattc cactggaaca tccggtaatt aagaccgatt tttgcataac tttacctgtc
    25261 aggatctccg ttgctttatg agtcatgatt tactaaaggc tgcaactgct tcgccatcca
    25321 gtcggcaata aacggctggg cgtcgcggtt gggatgaata ccgtcatcct gcatccattg
    25381 tggcttgagg tagacctctt ccataaaaaa gggcagcagc ggaacatcaa actctttggc
    25441 gagtttgggg taaatggcgc taaaggcttc attataacgg cgaccatagt ttgcaggcag
    25501 acgtatttgc attaacaatg gttcagcgtt ggcggctttg acatcctgca aaatctggcg
    25561 cagcgtttgc tcggtttgct gtggctgaaa accacgcaaa ccatcattgc gcccagttca
    25621 accagcaccc aacgcggctg atgctgtttg agcagagccg gaaggcgcgc cagcccttgt
    25681 tgcgaggtgt cgccgctgat gctggcatta actaccgacg ttttactctg ccacttatca
    25741 ttcaacaagg caggccaggc cgcgctggca gacattcgat acccggcgct caggctatca
    25801 cccagaatca ataacgtgtc cgctgcggcg gcacggaagg ttaacaggac caggaacagg
    25861 aagggcaaat gccagcggaa aacattgttg aagttcatca tcttaagaag tccgtcggtc
    25921 agggggagca tgaactctcc atcctcaccg gagttgagct ggttgtcaaa cgtggcgaga
    25981 ccatcgcact ggtgggcgag tcgggatcgg gtaagtcaac cttgctggcg atcctcgccg
    26041 ggcttgatga cggcagcagt ggcgaagtga gtctggtggg acaaccgcta cataatatgg
    26101 acgaagaagc gcgggcaaag ttgcgcgcga agcacgtcgg ctttgttttt cagtcattta
    26161 tgttaattcc tacccttaac gcgctggaaa acgtcgagct tccggctctg ctgcgcggtg
    26221 agagtagcgc ggaaagtcgt aacggggcga aagcgttgct cgaacagtta gggctgggta
    26281 aacgtctgga tcatcttccg gcacagcttt ccggcggtga acagcaacga gtggcgctgg
    26341 cacgagcctt taatggtcga cctgatgtgc tgtttgccga cgaacccacc ggcaaccttg
    26401 accgccagac gggcgataaa attgccgacc tgctgttttc cctcaaccgt gaacatggca
    26461 ccacgttgat tatggtgacc cacgacctgc aactggcggc acgctgcgac cgctgcttac
    26521 ggctggtgaa cgggcagttg caggaggaag catgattgca cgttggttct ggcgcgaatg
    26581 gcgttcgccg tcgctattaa ttgtctggct ggcgctaagc ctggcggtgg cctgcgtgct
    26641 ggcgctgggc aatatcagcg atcgcatgga gaagggctta agccagcaaa gccgtgagtt
    26701 tatggcgggc gatcgggcgt tgcgcagttc acgcgaagtg ccgcaagcgt ggctggagga
    26761 agcgcaaaag cgcggcctga aagtcggcaa gcagctgact ttcgccacaa tgacctttgc
    26821 aggcgacaca ccgcagctgg cgaacgtcaa agcggtggat gatatctacc cgatgtatgg
    26881 cggtctgcaa actaatcccc ctggcctgaa accgcaggcg ggcagcgtat tgctggcccc
    26941 acgcctgatg gcactgctta acctgaaaac gggcgacacc attgacgtgg gcgatgccac
    27001 cttgcggatt gccggagaag tgattcagga accggattcc ggttttaacc ccttccagat
    27061 ggctccgcgt ctgatgatga atctggcgga tgtcgataaa accggagccg tgcagccggg
    27121 gagtcgggtc acctggcgtt ataaattcgg cggcaacgag aaccagctcg acggctatga
    27181 gaaatggttg ttacctcagc ttaaacccga acaacgctgg tacggtctgg aacaggacga
    27241 aggcgcgctg gggcgatcga tggaacgctc gcaacagttc ctgctgcttt cggcgcttct
    27301 gaccttgctg ctggcagtgg cagcggtggc ggtagcgatg aatcattact gtcgcagtcg
    27361 ctacgatctg gtggcgatcc tcaaaacgct gggggcaggg cgagcgcaac tgcgtaagct
    27421 aatcgtcggt cagtggttga tggtgctgac gctttcagcc gttaccggtg gggccatagg
    27481 cctgttgttc gaaaacgtgt tgatggtgct gctcaagccc gttctgcctg ctgcactacc
    27541 gccagccagc ctctggccgt ggctgtgggc gcttggcacc atgacggtca tctcgctgct
    27601 ggtggggcta cgaccatacc gcttgttgct ggcaacgcag cctttacgcg tattacgtaa
    27661 tgatgtggtc gcgaacgtct ggccgctgaa gttttatctg ccgattgtca gtgtggtggt
    27721 tgtgctgctg ctcgccggat taatgggtgg cagcatgctg ctttgggcgg tgctggcggg
    27781 cgcggtagta ctggctttgc tgtgcggtgt gctgggctgg atgctgctga atgtacttcg
    27841 ccgcatgacg ctgaaatcgc tgcctctgcg cctggcggtt agccgcctgt tacgtcagcc
    27901 gtggtcaacg ttaagccagc tttcggcatt ttcgctctcc tttatgctgc tggcactgct
    27961 gctggtgctg cgtggcgatc tgctcgaccg ctggcagcag cagctaccgc cagaaagccc
    28021 gaactacttt ttaattaaca tcgccacaga acaggtagca ccgctaaaag cgttcctcgc
    28081 ggaacatcat atagtcccgg aatcatttta tccggtagtg cgggcgcggc tgacggcgat
    28141 taatgataag ccgacagaag gtaatgaaga tgaggcgctt aatcgcgaac tcaatcttac
    28201 ctggcaaaat acgcggcccg atcataatcc gattgtcgcc ggtaactggc cgccaaaagc
    28261 cgatgaagtg tcgatggaag aggggctggc gaaacgctta aacgttgccc tcggtgatac
    28321 cgtgactttt atgggcgata cccaggagtt ccgcgctaaa gtgaccagcc tgcgcaaagt
    28381 ggactgggaa agtctgcggc ctaatttcta ttttattttc cctgaagggg cattagacgg
    28441 gcaaccgcag agctggctta ccagtttccg ctgggagaat ggcaacggca tgttgacgca
    28501 actcaaccgc cagttcccga ccattagcct gttagacatt ggcgcgattt taaaacaggt
    28561 cggtcaggtg ctggagcagg taagtcgggc gctggaggtg atggtggtgc tggtcaccgc
    28621 ctgcggtatg ttgctgttgc tggcacaggt gcaggtggga atgcgccagc gtcatcagga
    28681 gctggtggta tggcgcacac tcggtgcggg gaaaaaactg ctgcgcacga cattgtggtg
    28741 tgatgttcgc catgcttggg tttgtttccg gcctggtggc cgcaattggt gcggaaacgg
    28801 cactggcagt gttgcaggcg aaagtgtttg atttcccgtg ggagccagac tggcgattgt
    28861 ggattgttct gccgtgcagc ggtgcgctgc tgctgtcgct tttcggcggc tggctgggtg
    28921 cgcgactggt taagggtaag gcgctgttca ggcagtttgt ggggtgatga caggtgttag
    28981 attgcctgat gcgctacgct tatcaggcct acgaagagcg tgcaatatat tgattttgca
    29041 agattgtgta ggccggataa ggcgtttacg ccgcatctgg catgaacagt gtgcattttg
    29101 gcaataaata tgaaaccaac tgtcatgtcg gtttttttaa aggccgaagg aggtaaacct
    29161 tcggccttag ctacgtttcc tcagttattc aataaactga cggatcagtt ccagcagcgc
    29221 gattagcgcc ttaagaacga tgagaacttt atcgagaaaa gttttcatcc gttgctccct
    29281 taagggagaa agacaatcac agcttaccct tacgttgcct ataactatga ttttcttcat
    29341 gtgctagatg aggactgtct ctctccaaat tagagcacca gaatcagcac cacctgtatc
    29401 ctgaccagga cttaaaagta cttttccgca ctgcggtaat cccttgaggc agtatcacta
    29461 ctagcgtcgg gattgtatcg ccaacatagc tgtttgaata tgtaggcatc ctgacttata
    29521 attcacttgc tagatgagga ctgtttaatt tctgcccgga accaccaagt cttgggcgga
    29581 aaaaataaaa aaaccgactg tcatgtcggt ttttttatat ctgtattctg gtgcaaccat
    29641 tttgttctca cgacaatcgc cgtttcagaa taccgcccat cactgcgcct taacccatac
    29701 cgccacttcc gcccactcac cgcgaaagac aactttttcc gcttttttct caagctgata
    29761 gcgatacatt gggtcgtaat attctttaag taacggcacc agccaggcca gatgaccgtc
    29821 ggtgctgccg gtggtgagtt gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt
    29881 atagcgctgt aaccccagcc gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca
    29941 atattcctgc cagccctgtt cgtcgccata agcgtgggta aaatcatgat gcatacgcaa
    30001 gaaatactct tcattcaggc gttcaagacg gatctcaaac ggatcttcta ccaccgcaat
    30061 cgccgcctga gtcattcgct cgcgcagaca ttccggcagg tgattcgaac cgatcatccg
    30121 gctttcatct tccagcaccc acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc
    30181 ggcagccagc aggttttcaa aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa
    30241 cgccgaaccg cgatgacgcg ccagcccttc cagatcaaca ccgtttggct gttgttgcac
    30301 taatagcgtt ttaccgctgc cggtacaacc gccaatcagc actatcggtt tttgtgccag
    30361 ttcaatggtc gcctgaatcg cggtctggcg cagtgcctta taaccgcctt ccaccagcgg
    30421 ataatcaatc cccgcttcat gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg
    30481 ggcgcagcag agaatacctt gcggattttg caggcacgct gcccgccagg cgtacatacg
    30541 ctgctgacga atttcacccg ccaccagttt atgccccagc gccagcgctg cgtctgagcc
    30601 ttgctgttta tagcaggtgc caacggaggc gcgttcatcg ttatccatta acggcagatt
    30661 gatagcggcg ggcattgcgc cttgctcaaa ctcgataggg gcgcgaacat caataatggg
    30721 cgtatcagca atcagcaggg cacgatagtc ctgttccgtg tgtctctctt gcatagttaa
    30781 aagtgaacct caaatcagct tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat
    30841 ttttaactgc gtggggtgcc gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg
    30901 gtaaacaagg tcacaatatc ttctaccgct ttgcagctgc caaatttgcg ccatgccaga
    30961 ctcaatggcg aaggagggcg catcgttggg attacccggc tgaccagttg ttgattatcg
    31021 atcattgact ggcaaagtga ttttggcaaa aaaccaatgc caacacccgc cagatgggcg
    31081 gcgattttcg tttccatatc gggaacaata atctcttttt gccccggcaa tcgccaggcg
    31141 acgcgtttgg ttaaggtgcc ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac
    31201 tgcgcttctg ttagcggctc ttcaacgttc gccagcggat gatccgccgc catgacaaat
    31261 cgccattgca ccgatcctaa gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc
    31321 gtgaccccga tagccagcga aaaaccttcg tacaatagcg agtcccagac gcccatatag
    31381 atttgtcggg agatgtgaaa ctgggtaaag gggtaacgct cattcagcca cgccagcaac
    31441 tgggcgacgg cctgggggtt atagagcagg ttgttgatga caatattcac ctggcgttcc
    31501 acgccatcat tcacctgttg cagctcgctg ggcatacttt ccagccagct cagccagtct
    31561 ctggcctggg aaagtagatg ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg
    31621 aaaaacagcg ctactccggt attctcttcc agaagtttaa tgcgataact gatcgtcgcc
    31681 gtggttttac ataatcgttc tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg
    31741 aaagtccgca aggtttctgg atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt
    31801 gcgtgatttt cttatcctct gatttatcag tatttttaca tgataatcct gttcaatttg
    31861 tggactaaat ctagttttgg aaaaacattc caacttttgt attaatgttg ttctcttaag
    31921 gttttagatt gcctgttatt gaaaccaagc tgaccggtcg gcggtggttg aacggaatta
    31981 tgttacaagg acaaaaagat gaaacttcag gtattaccgt taagtcagga agcctttagt
    32041 gcttatggcg acgtaatcga aacgcagcaa cgggattttt tccatattaa caatggcctg
    32101 gtggagcgtt accacgattt ggcgctggtt gagattcttg agcaagaccg tacgcttatc
    32161 agcattaacc gcgcgcaacc ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat
    32221 ccgctgggca ctcaggcctt tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg
    32281 ttaggcgacg acaaaccaga cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag
    32341 ggggtgaatt accatcgtaa cgtctggcat cacccacttt tcgcctggca gcgcgtcacc
    32401 gattttctga ccatcgatcg cggcggcagt gacaactgtg atgttgaaag tattcctgaa
    32461 caggaactct gttttgcgtg acgcctgcaa ccgacttgca taagataaac taattgttca
    32521 ttgtttatgc tcacttgtag gtcggagtta acgtaggtat gacggaagtt agacggcgcg
    32581 gcaggccagg acaggcggag cctgtggcac agaagggcgc acaggcgtta gagcggggaa
    32641 ttgcgattct gcaatatctg gaaaaaagtg ggggaagttc gtcggttagc gatatttctc
    32701 tcaatctgga tttgccgctc tccacgacct ttcgcttgct gaaggtttta caagcagcgg
    32761 attttgtcta tcaggacagt caattaggct ggtggcatat aggattaggt gtctttaacg
    32821 tcggtgcggc gtacatccat aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc
    32881 gcctgatgtt actttccggc gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg
    32941 tattaattgg tcagttagag tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc
    33001 gtctgccgct gcatgcttcc ggtgcgggca aagcgctgct ttatccgctg gcggaagagg
    33061 agttgatgag catcattctg caaaccggtt tgcagcagtt tacgccaact acgcttgtgg
    33121 atatgcccac cttgctgaag gacctggaac aagcgcgtga actgggctat accgtagata
    33181 aagaagagca tgttgtaggt ctgaattgca tagcttcagc aatttacgat gatgtcggta
    33241 gagttgttgc cgctatctcc atctccgggc cttcatcaag actgacagaa gatcgttttg
    33301 tcagtcaggg tgagctggtc agagacaccg cccgcgatat cagcacggcg ttgggactga
    33361 aagcacatcc atagtgtctg tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt
    33421 tgaaagttgg aaaatttttc caactaatag aggtaggaat aaaatggcaa aaatgagagc
    33481 cgttgacgcg gcaatgtatg tgctggagaa agaaggtatc accaccgcct tcggcgttcc
    33541 gggagctgca attaatccgt tctactcagc gatgcgtaag cacggcggta ttcgtcacat
    33601 tctggcgcgt catgtggaag gtgtttcgca catggcggaa ggttataccc gcgcaacggc
    33661 agggaatatc ggcgtatgtc tggggacttc cggtcctgcg ggcacggaca tgatcaccgc
    33721 gctctattcc gcttctgctg attccattcc tattctgtgc attaccggcc aggcaccgcg
    33781 cgcccgtctg cataaagaag attttcaggc cgtagatatt gaagcaattg ctaaaccggt
    33841 cagcaaaatg gcggttacag ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc
    33901 atttcacctg atgcgttctg gtcgtccggg tccggtactg gtggatttac cgttcgacgt
    33961 tcaggttgcg gaaatcgagt ttgatcctga catgtacgaa ccgctgccgg tctacaaacc
    34021 tgctgccagc cgtatgcaga tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc
    34081 ggtgattgtt gccgggggcg gggtaatcaa tgctgacgca gctgcactgt tacaacagtt
    34141 tgctgaactg accagcgttc cggtgatccc aacgctgatg ggctggggct gtatcccgga
    34201 cgatcatgaa ctgatggccg ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc
    34261 aacgttgctg gcgtccgaca tggtgtttgg tatcggtaac cgttttgcta accgtcatac
    34321 cggttcggta gagaaataca ccgaagggcg caaaatcgtt catattgata ttgagccgac
    34381 gcaaattggt cgcgtgctgt gtccggatct cggcattgtc tctgatgcta aagcggcgct
    34441 gacactgctg gttgaagtgg cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa
    34501 agaatgggtc gccgactgcc agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga
    34561 caacgtgccg gtgaaaccgc agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga
    34621 tgtttgttat gtcaccacta ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt
    34681 ctttaaagac cgccactgga tcaactgtgg tcaggctggt ccgttaggct ggacgattcc
    34741 ggcggcgctg gggggttgtg ccgctgatcc gaaacgcaat gtggtggcga tttctggcga
    34801 ctttgacttc cagttcctga ttgaagagtt agccgttggc gcgcagttca acattccgta
    34861 catccatgtg ctggtgaata acgcctatct cggcctgatt cgtcagtcac aacgcgcttt
    34921 tgacatggac tactgcgtgc aactcgcttt cgagaatatc aactccagtg aagtgaatgg
    34981 ctacggtgtt gaccacgtaa aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt
    35041 caaaccggaa gatattgcgc cagcctttga acaggcgaaa gccttaatgg cgcaatatcg
    35101 ggtaccggta gtcgtggaag ttattctcga gcgtgtgacc aatatttcga tgggcagcga
    35161 actggataac gtcatggaat ttgaagatat cgccgataac gcagcggatg caccgactga
    35221 aacctgcttc atgcactatg aataagggag ataaatgatg ttacgtttct ctgctaattt
    35281 atcgatgtta tttggtgaat atgattttct cgcccgtttt gagaaagctg cgcagtgtgg
    35341 ttttcgcggc gttgaattta tgtttcctta tgactacgac attgaagaat taaaacaggt
    35401 actggcgagt aataaactcg aacatacgct gcacaattta ccggcaggtg actgggcggc
    35461 gggcgagcgc ggtattgcct gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc
    35521 agcagcgatt
//
LOCUS       DADLTY010000019        34903 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.019, whole genome shotgun sequence.
ACCESSION   DADLTY010000019 DADLTY010000000
VERSION     DADLTY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.019"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            858..3143
                     /gene="nrdA"
                     /locus_tag="JN334_000794"
     CDS             858..3143
                     /gene="nrdA"
                     /locus_tag="JN334_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAY7437041.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3377..4507
                     /gene="nrdB"
                     /locus_tag="JN334_000795"
     CDS             3377..4507
                     /gene="nrdB"
                     /locus_tag="JN334_000795"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HAY7437042.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4507..4761
                     /gene="yfaE"
                     /locus_tag="JN334_000796"
     CDS             4507..4761
                     /gene="yfaE"
                     /locus_tag="JN334_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HAY7437043.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="JN334_000797"
     CDS             complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="JN334_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HAY7437044.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5644..5886
                     /locus_tag="JN334_000798"
                     /pseudo
     CDS             5644..5886
                     /locus_tag="JN334_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="JN334_000799"
     CDS             complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="JN334_000799"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY7437045.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="JN334_000800"
     CDS             complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="JN334_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HAY7437046.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8640..10268
                     /gene="glpA"
                     /locus_tag="JN334_000801"
     CDS             8640..10268
                     /gene="glpA"
                     /locus_tag="JN334_000801"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HAY7437047.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10258..11517
                     /gene="glpB"
                     /locus_tag="JN334_000802"
     CDS             10258..11517
                     /gene="glpB"
                     /locus_tag="JN334_000802"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HAY7437048.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11514..12704
                     /gene="glpC"
                     /locus_tag="JN334_000803"
     CDS             11514..12704
                     /gene="glpC"
                     /locus_tag="JN334_000803"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HAY7437049.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12897..13847
                     /locus_tag="JN334_000804"
     CDS             12897..13847
                     /locus_tag="JN334_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY7437050.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13888..14358)
                     /locus_tag="JN334_000805"
     CDS             complement(13888..14358)
                     /locus_tag="JN334_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY7437051.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14413..15615)
                     /locus_tag="JN334_000806"
     CDS             complement(14413..15615)
                     /locus_tag="JN334_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HAY7437052.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15702..16244)
                     /locus_tag="JN334_000807"
     CDS             complement(15702..16244)
                     /locus_tag="JN334_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437053.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16523..16948
                     /gene="nudI"
                     /locus_tag="JN334_000808"
     CDS             16523..16948
                     /gene="nudI"
                     /locus_tag="JN334_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HAY7437054.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16987..17589)
                     /locus_tag="JN334_000809"
     CDS             complement(16987..17589)
                     /locus_tag="JN334_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAY7437055.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFG
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17897..19036
                     /gene="arnB"
                     /locus_tag="JN334_000810"
     CDS             17897..19036
                     /gene="arnB"
                     /locus_tag="JN334_000810"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HAY7437056.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19040..20008
                     /gene="arnC"
                     /locus_tag="JN334_000811"
     CDS             19040..20008
                     /gene="arnC"
                     /locus_tag="JN334_000811"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HAY7437057.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            20008..21989
                     /gene="arnA"
                     /locus_tag="JN334_000812"
                     /pseudo
     CDS             20008..21989
                     /gene="arnA"
                     /locus_tag="JN334_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21986..22876
                     /locus_tag="JN334_000813"
     CDS             21986..22876
                     /locus_tag="JN334_000813"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HAY7437058.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22876..24528
                     /gene="arnT"
                     /locus_tag="JN334_000814"
     CDS             22876..24528
                     /gene="arnT"
                     /locus_tag="JN334_000814"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HAY7437059.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24525..24860
                     /gene="arnE"
                     /locus_tag="JN334_000815"
     CDS             24525..24860
                     /gene="arnE"
                     /locus_tag="JN334_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HAY7437060.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24860..25246
                     /gene="arnF"
                     /locus_tag="JN334_000816"
     CDS             24860..25246
                     /gene="arnF"
                     /locus_tag="JN334_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HAY7437061.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25240..25512)
                     /gene="pmrD"
                     /locus_tag="JN334_000817"
     CDS             complement(25240..25512)
                     /gene="pmrD"
                     /locus_tag="JN334_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HAY7437062.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25617..26972)
                     /gene="menE"
                     /locus_tag="JN334_000818"
     CDS             complement(25617..26972)
                     /gene="menE"
                     /locus_tag="JN334_000818"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HAY7437063.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26969..27931)
                     /gene="menC"
                     /locus_tag="JN334_000819"
     CDS             complement(26969..27931)
                     /gene="menC"
                     /locus_tag="JN334_000819"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HAY7437064.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27931..28788)
                     /gene="menB"
                     /locus_tag="JN334_000820"
     CDS             complement(27931..28788)
                     /gene="menB"
                     /locus_tag="JN334_000820"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HAY7437065.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28803..29561)
                     /gene="menH"
                     /locus_tag="JN334_000821"
     CDS             complement(28803..29561)
                     /gene="menH"
                     /locus_tag="JN334_000821"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HAY7437066.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29558..31228)
                     /gene="menD"
                     /locus_tag="JN334_000822"
     CDS             complement(29558..31228)
                     /gene="menD"
                     /locus_tag="JN334_000822"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HAY7437067.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31317..32612)
                     /gene="menF"
                     /locus_tag="JN334_000823"
     CDS             complement(31317..32612)
                     /gene="menF"
                     /locus_tag="JN334_000823"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HAY7437068.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32691..32996)
                     /gene="elaB"
                     /locus_tag="JN334_000824"
     CDS             complement(32691..32996)
                     /gene="elaB"
                     /locus_tag="JN334_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HAY7437069.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33051..33512)
                     /locus_tag="JN334_000825"
     CDS             complement(33051..33512)
                     /locus_tag="JN334_000825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7437070.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33577..34494
                     /gene="rbn"
                     /locus_tag="JN334_000826"
     CDS             33577..34494
                     /gene="rbn"
                     /locus_tag="JN334_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HAY7437071.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34685..34873
                     /locus_tag="JN334_000827"
                     /pseudo
     CDS             34685..34873
                     /locus_tag="JN334_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         8536 a         9341 c         8648 g         8378 t
ORIGIN      
        1 cgtaatgcct gaaaggcttt gcctgctggc gtgacaactc gctttgatat catatccctg
       61 gcataaatca atggccgccg cgacaccgtt agcagagaaa agagatgcaa tcattgacgg
      121 gagtagagat aaatactcct tgcgtagaaa gataatccgc atattaatct gaccatcatt
      181 ttatataaga cggcatattt aaacgcatta tacaatatgg gttatctttc ttttgtaaat
      241 tgtccaaccc gatgcaatgg ctattttgtt attatttgat gaataatatc agtgcgtcat
      301 aattcaagtt aataacctta agggatacca gttatattta aactaaatta aagtaatgaa
      361 taattttctt ataatataag gtaaattaac aaaatggctt cgcatttaac aataaccgaa
      421 tagaaaacaa ccatttcgac atcaacaatc gcttacattc gcttatatat tgaccacaac
      481 tgatacatca gattatgtga tgactcgtgc ttagatcaat ttttgcaatc attagcaaaa
      541 agattaataa accatctata tcaatttatc caacctattc tgccgttcaa gaaatcgccg
      601 aacagttatt tttaacaaat ttttctcttc ccattgactt tcccggacac cttgtctgac
      661 ctaaggtgcg cgaaagccac tttttccttc ctgagttatc cacaaagtta tgcacttgca
      721 agagggtcat tttcacacta tcttgcagtg aatcccaaac atacccccta tatatagtgt
      781 tctaagcagc ttcccttact acaggtagtc tgcatgaaac tattgcggaa agaattccaa
      841 aaacaggtac gacatacatg aatcagaatc tgctggtgac aaagcgcgac ggtagcacag
      901 agcgcatcaa tctcgacaaa atccatcgcg ttctggattg ggcggcagaa ggactgcata
      961 acgtttcgat ttcccaggtc gagctgcgct cccacattca gttttatgac ggtatcaaga
     1021 cctctgacat ccacgaaact attatcaagg ctgccgcaga cctgatctcc cgtgatgcgc
     1081 cggattatca gtatctcgcc gcgcgcctgg cgatcttcca cctgcgtaaa aaagcctacg
     1141 gccagtttga gccgcctgcg ctgtacgacc acgtggtgaa aatggtcgag atgggcaaat
     1201 acgataatca tctgctggaa gactacacgg aagaagagtt caagcagatg gacacgttta
     1261 tcgatcacga ccgtgatatg accttctctt atgctgccgt taagcagctg gaaggcaaat
     1321 atctagtaca gaaccgcgtg accggcgaaa tctacgaaag cgcccagttc ctttatattc
     1381 ttgttgccgc gtgcctgttc tcgaactacc cgcgtgaaac gcgcctgcaa tacgtgaagc
     1441 gtttttatga cgcggtttcc acatttaaaa tttcgctgcc gacgccaatc atgtccggcg
     1501 tgcgtacccc gactcgtcag ttcagctcct gcgtactgat cgagtgcggt gacagcctgg
     1561 attccatcaa cgccacctcc agcgcgattg tgaaatatgt ttcccagcgt gccgggatcg
     1621 gcatcaacgc cggacgtatt cgtgcgctgg gcagcccgat tcgcggtggc gaagcgttcc
     1681 ataccggctg tatcccgttc tacaaacatt tccagacagc ggtgaaatcc tgctctcagg
     1741 gcggtgtgcg cggcggtgcg gcaacgctgt tctacccgat gtggcatctg gaagtggaaa
     1801 gcctgctggt gttgaaaaac aaccgtgggg ttgaaggcaa ccgcgtgcgt catatggact
     1861 acggggtaca aatcaacaaa ctgatgtata cccgcctgct gaaaggtgaa gatatcaccc
     1921 tgttcagccc gtccgacgta ccggggctgt acgacgcgtt cttcgccgat caggaagagt
     1981 ttgaacgcct gtataccaaa tatgagaaag acgacagcat ccgcaagcag cgtgtgaaag
     2041 ccgttgagct gttctcgctg atgatgcagg aacgtgcgtc taccggtcgt atctatattc
     2101 agaacgttga ccactgtaat acccatagtc cgtttgatcc gaccatcgcg ccagttcgcc
     2161 agtctaacct gtgcctagaa attgccctgc cgaccaaacc gctgaacgac gtcaacgacg
     2221 agaacggtga aatcgcgctg tgtacgctgt ctgctttcaa cctgggcgca attaatagcc
     2281 tggatgaact ggaagagctg gcgattctgg cggttcgtgc acttgacgcg ctactggatt
     2341 atcaggatta cccgatcccg gccgccaaac gtggagcgat ggggcgtcgt acgctgggta
     2401 ttggtgtgat taacttcgct tactatctgg cgaagcacgg taaacgctac tccgacggca
     2461 gcgccaacaa cctgacgcat aaaaccttcg aagcgattca gtattacctg ctgaaagcgt
     2521 ctaatgagct ggctaaagag caaggcgcgt gcccgtggtt taacgaaacc acttacgcga
     2581 aagggatcct gccgatcgat acctataaga aagatctgga tgccatcgct aatgagccgc
     2641 tgcattacga ctgggaagca ttgcgtgaat caatcaaaac gcacggcctg cgtaactcca
     2701 cgctttctgc cctgatgccg tccgagacct cttcgcagat ctctaacgcc actaacggta
     2761 ttgaaccgcc gcgcggttac gtcagcatca aagcgtcgaa agacggtatt ttgcgccagg
     2821 tggtgccgga ctacgagcac ctgcacgacg cctatgagct gctgtgggaa atgccgggta
     2881 acgatggcta cctgcaactg gtaggtatca tgcagaaatt tatcgatcag tcgatctctg
     2941 ctaacaccaa ctacgatccg tcacgtttcc cgtcaggaaa agtgccgatg cagcagttgc
     3001 tgaaagacct gctcaccgcc tacaaattcg gggtcaaaac gctgtattat cagaacaccc
     3061 gtgacggcgc tgaagacgcg caggacgatc tggtaccgtc aatccaggac gatggctgcg
     3121 aaagcggcgc atgtaagatc tgatattgag atgccggatg cggcgtaaac gccttatccg
     3181 gcctacggct cggtttgtag gcctgataag acgcgccagc gtcgcatcag gctccgggtg
     3241 ccggatgcag cgtgaacgcc ttatccggcc tacggctcgg atttgtaggc ctgataagac
     3301 gcgccagcgt cgcatcaggc acaggatgcg gcgtaaaatg ccttatccgg cattaaactc
     3361 ccaacaggac acactcatgg catataccac cttttcacag acgaaaaatg atcagctcaa
     3421 agaaccgatg ttctttggtc agccggtcaa cgtggctcgc tacgatcagc aaaaatatga
     3481 catcttcgaa aagctgatcg aaaagcagct ctctttcttc tggcgtccgg aagaagttga
     3541 cgtctcccgc gaccgtatag attaccaggc gctgccggag cacgaaaaac acatctttat
     3601 cagcaacctg aaatatcaga cgctgctgga ttccattcag ggtcgtagcc cgaacgtggc
     3661 gctattgccg cttatttcta ttccggaact ggaaacctgg gtcgaaacct gggcgttctc
     3721 agaaacgatt cattcccgtt cctatactca tatcattcgt aatatcgtta acgatccgtc
     3781 tgttgtgttt gacgatatcg tcaccaacga gcagatccag aaacgtgcgg aagggatctc
     3841 cagctattac gatgaactga tcgaaatgac cagctactgg caccttctgg gcgaaggcac
     3901 ccacaccgtt aacggtaaaa ccgtaaccgt tagcctgcgc gagctgaaga aaaaactgta
     3961 tctctgcctg atgagcgtta acgcgctgga agcgatccgc ttctacgtca gctttgcttg
     4021 ttcctttgcc tttgcagaac gcgaactgat ggaaggtaac gccaagatta ttcgcctgat
     4081 tgcccgcgat gaagccctgc acctgaccgg tactcagcat atgctgaatc tgctgcgcag
     4141 cggcgcggat gatccggaga tggcggaaat tgccgaagaa tgtaagcagg agtgctatga
     4201 cctgtttgtc caggcggcac agcaagagaa agactgggcg gattatctgt tccgtgacgg
     4261 ttcgatgatt ggtctgaata aagatattct ctgccagtac gttgaataca tcaccaatat
     4321 ccgcatgcag gcagtcggtt tggatctgcc gttccagacg cgctccaacc cgatcccgtg
     4381 gatcaacacc tggctggtgt ctgataacgt gcaggttgct ccgcaggaag tggaagtcag
     4441 ttcttatctg gtcgggcaga ttgacgcgga agtggacacc gacgatttga gtaacttcca
     4501 gctctgatgg cccgcgttac cctgcgcatc actggcacac aactgctgtg ccaggatgaa
     4561 cacccttccc ttctggcggc gctggaatcc cacaatgtgg cggttgagta ccagtgccgc
     4621 gaaggttact gcggctcctg tcgcacccga ctggtcgcag gtcaggtgga ctggattgcc
     4681 gaaccgttag cctttattca gccgggggaa attttgccct gttgttgccg ggcaaaaggc
     4741 gatattgaaa tcgagttgtg atcgggtact agcgccagat acgacgctta cgcgtattat
     4801 ctggcctaca acgattacat ggcgtagtaa tacgctttca cctgctccca gtccgcttta
     4861 gggatcggct ccagatattt ttccagctgg cggaagtcat gattaatcgc tttatcgcga
     4921 cgcaagcggc gacgactttt ctccagatca agaaaaccag cttctgcctt accttctgtt
     4981 ttcacataga tatggcgaac atagcaacag ccatgctgac gattaacgct atgcattttc
     5041 ttaaacgcca gtgccactgc tttcaacatg gcttgccgta cttcgtcaga ataaggcgat
     5101 actgcatgcc gggcatacca gtcagcaatg ctgatgaacc ccgccatatc ttcagtcacc
     5161 agcaacgcgc gccattcacc ctcaattttc accgcttcac caaaaacgat cttcggcacg
     5221 atgacaccgg cccgttccag ttctttaatt accgcaacct cacggacaat cgttggtcgg
     5281 ccgaacggat aacgtactga atgaaacaga tgatgcgtca tgcgctttac atacagcttt
     5341 ttgccgttgc gctcgacgca ttgcaccccg ctcataccat tacggcgata gttaggctct
     5401 tcaacccagt cgccctctgt tgcccaccag tgattaaatt cgtcgtactt tgctgaaact
     5461 gccataccct atcgcctgtc atttttatta acgacaatga ctataggtgg ttacctgagg
     5521 aaaatcttaa tgaaacgtgt cgtattaatg aatttctttt gccgctacag gtgtgacacc
     5581 gcttttgccc ttcatttgca gcgcatagat tccaccagcc actaccacta atagtgcgcc
     5641 cagcagataa aatgtgaatt taatcagtcg gatacagcga tgaattttat tcgacaggga
     5701 ttaggcatcg ccttacaacc agagttaacg ctgaaaagca ttgcaggtga attgtgttcc
     5761 gttcctcacg aaccaacctt ctatcgacag atttcgttgc tgactaaaga aaagccggta
     5821 gaaggcagtc cactgttttt actacaaatg tgcatggaac aattagtggc gattggaaaa
     5881 atttgatatg agacaggatg gcgcatgaac gccatcctgc agtaacatta ctctttatta
     5941 agaaatttta ctgccttatc agggaaatca gtaaacagcc catttacacc cgctttgtta
     6001 tacagagcat catataactg attcacatca ggagtgtatt caggcagttt atctgaccgc
     6061 acggtataag gatgcactac cagtttattc tgctgggcat cttgcaccat gccagtgagt
     6121 ttgatattac ccggctgcga tgtctcctca atcaacatat ggtaatccgg accaatacca
     6181 tctgcatatt ccgccacctg tttcatggct cccggcttaa acatccagtc gtagttgtaa
     6241 ttaacccagc ttccatccgg ctgtttctgc tgcgtttcat tccagtcggt ataggcaatc
     6301 agctgtacca gattgagctc catgcccatt ttgggttcca gctcattctt aatacgcttc
     6361 agctcatcag catcaaaaca ttgcaaataa actttatcgt ctttaccggt gtaaccatat
     6421 ttcttcagca cttccagcgt ttttgccgca atatccttcc cttcctgatg atggaaccac
     6481 ggcgctttga tttctggata gataccgata tttttcccgg tagagtgatt taacccctga
     6541 acaaattcaa tctcttcttc aaaggtgtgc acccggaagt cggacttacc cattgggaaa
     6601 cgccccggat aagtctgtac ttttttaccg ttttcaatat cgaaaccttc ggtaaatttc
     6661 agcgacttaa tttcatccag cgtgaaatct atcgcgtagt aacgaccgtc tttgcgcgcc
     6721 cgatccggga aacgatcggc aacatcagta acacgatcga gataatggtc atgcagaaca
     6781 accagatggt cgtctttggt catcaccaaa tcctgttcca gataatccgc tccctgcgca
     6841 tacgccatcg cttttgctgg cagcgtatgc tccggcaaat atccactggc accgcgatgg
     6901 gcgatgacta ttttttcgtt gctgtccgcc gccattgcac tgcttcccat gactatagtg
     6961 ctcatcatga tcgccatgct aaggtttttc agcttcaatt tcatgccatt agcctccgtt
     7021 gcgtttttgc agtaattgtt catggcgacg tttttcgcca atcatcacaa caatcaacaa
     7081 gataaccgcc agaatgctgc cgccaatcat taccataaag ccgccatccc agccgaagaa
     7141 gtccacggtg tagccaacaa tcgcgctcgc cgccaccgaa ccgcccaggt aaccaaacag
     7201 cccggtaaag cccgctgccg tacctgccgc ttttttcggt gccagttcca gcgcatgcag
     7261 accgatcagc atcacaggac cgtagatcag gaagccgata acaatcatac aaatcatatc
     7321 gacggttggg ttacctgccg ggttcatcca gtaaacgata gtcgcgatgg tcaccagtgt
     7381 cataaagaag acgccggttg ccccacggtt accacggaag actttgtccg acatccagcc
     7441 gcacagcagg gtgcccggaa tacccgcata ctcatagaag aagtaggccc aggaggattt
     7501 atccagcgcg aaatgcttaa cctctttcag ataagtcggt gaccagtcga ggatgccgta
     7561 acgcagcaga taaacgaaca cgttggcgat ggcgatgtac cacagcagtt tgttcggcag
     7621 cacgtactgc atgaagattt gcttcgcggt cagctcctgt tccgcttttt cgttatagtc
     7681 gtccggataa tcatttttgt actcttcgat cggcggcaag ccacaggatt gcggggtatc
     7741 gcgcatcatc gcaaaggcga ataatgccac cagaatggcg cagaaagcag gcatatagag
     7801 cgccgcatgc cagtcattga accaggccat ccccagcagg aacagcagcg gcggaatacc
     7861 accaccgacg ttgtgcgcac agttccacac tgacacaatg ccgccacgtt ctttctgcga
     7921 ccaccagtgc accatagtac gaccacacgg cggccacccc atcccctgga accaaccgca
     7981 gaggaacaac agtacaaaca tcaccgcaat gctcgacgtc gcccatggca caaagcccat
     8041 aaacaacatc actgccgccg ccagaatcaa acctgcgggc aggaaaacgc gcggattcga
     8101 gcgatccgat accgaaccca tgatgaattt cgaaaatcca taagcaatcg agatccccga
     8161 aagggcaaaa cctaaatcac cgcgtgagaa tccctgctca accagataag gcatagcaag
     8221 cgcaaagttc ttacgaacca aatagtaagc cgcatagcca aagaatatcc ccaggaaaat
     8281 ttgccagcgc aatcgacgat aagtcggatc gatctccgcg gcaggtaagc gcgctttgtg
     8341 tggcgctggt ttaaaaatac tcaacattga tagcctccgt ggcccgtggt cttatttatg
     8401 attaacagcc tgattcagtg agagaacctg ccgtttcttg agttgccgcg atgttaagaa
     8461 aacattcata aattaaatgt gaattgccgc acacattatt aaataagatt tacaaaatgt
     8521 tcaaaatgac gcatgaaatc acgttttact ttcgaatcat gagcgaatat gcgcgaaatc
     8581 aaacaattca tgtttttact atggctaaat ggtaaaaaac gaacttcaga gggataacaa
     8641 tgaaaactcg cgactcgcaa tcaagtgacg tgattatcat tggcggcggc gcaacgggag
     8701 ccgggattgc ccgcgactgt gccctgcgcg ggctgcgcgt gattttggtt gagcgccacg
     8761 acatcgcaac cggtgccacc gggcgtaacc acggcctgct gcacagcggt gcgcgctatg
     8821 cagtaaccga tgcggaatcg gcccgcgaat gcattagtga aaaccagatc ctgaaacgca
     8881 ttgcacgtca ctgcgttgaa ccaaccaacg gcctgtttat caccctgccg gaagatgacc
     8941 tctcattcca ggccactttt attcgcgcct gcgaagaagc agggatcagc gcagaagcta
     9001 tagacccgca gcaagcgcgc attatcgaac ctgccgttaa cccggcactg attggcgcgg
     9061 tgaaagttcc ggatggcacc gttgatccat ttcgtctgac cgcagcaaac atgctggatg
     9121 ccaaagaaca cggtgccgtt atccttaccg ctcatgaagt cacggggctg attcgtgaag
     9181 gcgcgacggt gtgcggtgtt cgtgtacgta accatctcac cggcgaaact caggcccttc
     9241 atgcacctgt cgtggttaat gccgctggga tctgggggca acacattgcc gaatatgccg
     9301 atctgcgcat tcgcatgttc ccggcgaaag gatcgctgct gatcatggat caccgcatta
     9361 accagcatgt gatcaaccgc tgccgtaaac cttccgacgc cgatattctg gtgcctggcg
     9421 ataccatttc gctgattggt accacctctt tacgtattga ttacaacgag attgacgata
     9481 atcgagtgac ggcagaagag gttgatattc tgctgcgtga aggggaaaaa ctggcccccg
     9541 tgatggcgaa aacgcgcatt ttgcgggcct attctggcgt gcgcccgctg gttgccagcg
     9601 atgacgaccc gagcggacgt aacgtcagcc gtggcatcgt gctgctcgac catgctgaac
     9661 gcgatggtct ggacggattt atcaccatca ccggtggcaa actgatgacc tatcggctga
     9721 tggctgaatg ggctaccgac gcggtatgcc gcaaactggg caacacgcgc ccctgtacga
     9781 ctgccgatct ggcactgcct ggttcacaag aacccgctga agttaccttg cgtaaagtca
     9841 tctccctgcc tgccccgctg cgcggttctg cggtttatcg tcatggcgat cgcacgcctg
     9901 cctggctgag cgaaggccgt ctacaccgta gcctggtatg tgagtgcgaa gcggtaactg
     9961 cgggtgaagt gcagtacgcg gtagaaaatt taaacgttaa tagcctgctg gatttacgcc
    10021 gtcgtacccg tgtggggatg ggcacctgcc agggcgaact ctgcgcctgc cgcgctgccg
    10081 gactgctgca acggtttaac gtcacgacgt ccgcgcaatc tatcgagcaa ctttccacct
    10141 tccttaacga acgctggaaa ggcgtgcaac ccatcgcctg gggagacgca ctgcgcgaaa
    10201 gcgaatttac ccgctgggtt tatcagggac tgtgtggtct ggagaaggag cagaaagatg
    10261 cgctttgata ctgtcattat gggcggcggc ctcgccggat tactctgtgg cctgcaactg
    10321 caaaaacacg gcctgcgctg tgccattgtc acgcgtggtc aaagcgcgtt gcatttctca
    10381 tccggttcgc tggatttgct gagccatctg ccagatggtc aacccgtggc agacattcac
    10441 agtggactgg aatctttgcg tcagcaggca cctgcacatc cttactctct tctcgggcca
    10501 caacgcgtgc tcgatctcgc ttgccaggcg caggcattaa tcgctgaaag cggtgcgcaa
    10561 ttgcagggca gcgtagaact ggctcaccag cggattacgc cgctcggcac tttgcgtgca
    10621 acctggttaa gttcgccaga agttcccgtc tggccgctac ccgcgaagaa gatatgtgta
    10681 gtggggataa gcgggctgat ggatttccag gcgcatcttg cggcggcttc gttgcgcgaa
    10741 ctggatcttt cggttgaaac cgcagaaata gagctgccgg aactggatgt cctgcgcaat
    10801 aacgccaccg aatttcgcgc ggtgaatatc gcccgtttcc ttgataatga agaaaactgg
    10861 ccgctgttac ttgatgcgct tattcctgtc gccaatacct gcgaaatgat cctgatgccc
    10921 gcctgcttcg gcctggccga tgacaaactg tggcgttggt tgaatgaaaa actaccttgt
    10981 tcactgatgc ttttgccaac gctgccgcct tccgtgctgg gcattcgtct gcaaaaccag
    11041 ttacagcgcc agtttgtgcg ccaaggtgga gtgtggatgc cgggcgatga agtgaaaaaa
    11101 gtgacctgta aaaatggcgt agtgaatgaa atctggaccc gcaatcacgc cgatattccg
    11161 ctacgtccac gtttcgcggt tctcgccagc ggcagtttct ttagtggcgg actagtagcg
    11221 gaacgtaacg gcattcgcga gccgattctc ggccttgatg tgctacaaac cgccacgcgg
    11281 ggtgaatggt ataagggcga tttttttgcg ccgcaaccgt ggcagcagtt cggtgtaacc
    11341 actgatgaga cgctacgccc gtcgcaggca gggcaaacca ttgagaacct gtttgccatc
    11401 ggttcggtgc tgggcggatt tgatcccatc gcccagggat gcggcggcgg tgtttgtgcc
    11461 gtcagtgctt tacatgccgc tcaacagatt gcccaacgcg caggaggcca acaatgaacg
    11521 acaccagctt cgaaaactgc attaagtgca ccgtctgcac caccgcctgc ccggtgagcc
    11581 gggtgaatcc cggttatcca gggccaaaac aagccgggcc ggatggcgag cgtctgcgtt
    11641 tgaaagatgg cgcactgtat gacgaggcgc tgaaatattg catcaactgc aaacgttgtg
    11701 aagtcgcctg cccgtccgat gtgaagattg gcgatattat ccagcgcgcg cgggcgaaat
    11761 atgacaccac gcgcccgtcg ctgcgtaatt ttgtgttgag tcataccgac ctgatgggta
    11821 gcgtttccac gccgttcgca ccaatcgtca acaccgctac ctcgctgaaa ccggtgcggc
    11881 agctgcttga tgcggcgtta aaaatcgatc atcgtcgcac gctaccgaaa tactccttcg
    11941 gcacgttccg tcgctggtat cgcagcatag cggctcagca agcacaatat aaagaccagg
    12001 tcgctttctt tcacggctgc ttcgttaact acaaccatcc gcagttaggt aaagatttaa
    12061 ttaaagtgct caacgcaatg ggtaccggtg tacaactgct cagcaaagaa aaatgctgcg
    12121 gcgtaccgct aatcgccaac ggctttaccg ataaagcacg caaacaggca attacgaatg
    12181 tagagtcgat ccgcgaagct gtgggagtaa aaggcattcc ggtgattgcc acctcctcaa
    12241 cctgtacatt tgccctgcgc gacgaatacc cggaagtgct gaatgtcgac aacaaaggct
    12301 tgcgcgatca tatcgaactg gcaacccgct ggctgtggcg caagctggac gaaggcaaaa
    12361 cgttaccgct gaaaccgctg ccgctgaaag tggtttatca cactccgtgc catatggaaa
    12421 aaatgggctg gacgctctac accctggagc tgttgcgtaa catcccgggg cttgagttaa
    12481 cggtgctgga ttcccagtgc tgcggtattg cgggtactta cggtttcaaa aaagagaact
    12541 accccacctc acaagccatc ggcgcaccac tgttccgcca gatagaagaa agcggcgcag
    12601 atctggtggt caccgactgc gaaacctgta aatggcagat tgagatgtcc acaagtcttc
    12661 gctgcgaaca tccgattacg ctactggccc aggcgctggc ttaaactccc ttctgatgcc
    12721 cggtaagcat gtggttaccg ggcatttttg cgtacacgat tccgtgccca atgtatgcgt
    12781 tgcaacgcag tgaaaattcc tctgaaaacg tctcgcaaag gctgaaactg gcagatgtca
    12841 aaggcctggg ataaccgtaa tgtcgcgtca tcataaatat caggtgacgg acaaccatga
    12901 ccgaatcaac aacctcctcc ccgcatgatg cggtatttaa aacctttata ttcacacccg
    12961 aaaccgcacg ggattttctc gaaatacatt taccagaacc actgcgcaag ctttgcaacc
    13021 tgcaaacctt acgcctggaa cccactagtt ttattgaaaa aagtttacgc gcttactact
    13081 cggatgtttt gtggtccgtg gaaaccagcg acggtgacgg ctatatctac tgcgtgattg
    13141 aacatcaaag ctctgcagaa aagaatatgg cttttcggcc aatgcgctat gccactgccg
    13201 ccatgcagag tcacctagat aaaggctatg acagagttcc gctggtggtg ccattgctgt
    13261 tttatcatgg cgaaacatcg ccctacccgt actcacttaa ctggctggat gagtttgacg
    13321 atccgcaact tgcccggcag ttgtacaccg aagcttttcc gttggtggat atcaccatcg
    13381 tacctgacga tgagatcatg caacatcggc gtatagctct gctggaactg attcaaaagc
    13441 atattcgcga ccacgattta atcggcatgg tcgacaggat caccacgctt ttggttagag
    13501 gcttcactaa tgacagccag ctacaaacac tgtttaatta tctgctgcaa tgcggcgata
    13561 cctcccgttt cacccgtttt attcaggaga ttgccgaacg ttcaccacta caaaaggaga
    13621 gattaatgac tattgctgaa cggctacggc aggaaggaca tcaaattggc tggcaagaag
    13681 gtaaattagt aggtttgcag caaggtaaat tagaaggttt gcaggaaggt atgcatgaac
    13741 aagccattaa aattgctttg cgcatgctgg aacagggcat tgatcgtgac caagtgctcg
    13801 cggccaccca gctaagcgaa gccgatctgg cagcgaataa ccactaatta acacaggccc
    13861 acagccgatc cccatgggcc tttgatatca ataactacct tttatgcgtg gcccattttt
    13921 gcctgattta aacatcgcca gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac
    13981 gccaacagcg acaaagttcg ctccccacgc caggcattgc tgcgccatat caggagccac
    14041 agccagaaaa ccagccgctt tacccgcagc acggatccgc cgaatgctgg tttcaataat
    14101 tcgctgcact tccgggtgcc cggcgttatc cgggtagccc aacgacgcag aaagatccgc
    14161 tggtccaata aacacgccat caatcccttc gacgtcgagg atctcgtcca ggttatccag
    14221 tgccgtttta ctttccacct gcactaacag acaaagcgaa tcgttgactt gcgccatgta
    14281 attctcaatg cgtccccagc gcgcagcccg tgccacactg gccccaacac cacgctcacc
    14341 gtagggaggg ttggacaaat tttgccggag ggggaatgtg ccggtgcagt aaaccggcac
    14401 agagtggcga aatcagacgg ataaagtcat ggactcaaca acctcaatcc agccatgctc
    14461 actagcgatg tcctggccgt ttaaccaacg gcgtagcata ttcagtgcca tcattgcgca
    14521 cacttcctga cggatagcca ggctgtagcg tgtagtgctg aaacgcacac gcagagcgaa
    14581 agtgccgtct ggcgtggcta gcgcaaagtt gagatgctcg ttctcgaaac ccgaaacagc
    14641 cagtgccagc ccggcaaaat ggttggcccg ccgttctgta atccagtgcg cagtttgcgc
    14701 cagggtttct tcctgtgaag gaatcacttc acacgccagc aatggagcac ctgcgcgaga
    14761 aagttgcaaa gccaataaac caccggtgaa ttgctcgctc aacgtcaggc tgaactggcg
    14821 gttttgcaat tcgcgactga tctgcgcggg cagtccttcg gtgccttcga aaatcacgct
    14881 ctgtccggca acacgtttaa cgtccagcca cagtttttcc atcgcctgtt gctcgcttgc
    14941 cggtccggtg agtttcagtt cgatgatcgg cattgaggag cgatagccca ttgttacgcc
    15001 cggcggcagt tgtagagtgt ccaggctttg tgccagatcg ctttccgaac gaccaaaggt
    15061 agttaaacgc agacaaaccg gcggctgtgg taaagaaaaa cgctcgcgca ggcgcggcag
    15121 gatttcgtgc tcgaccatca ccttaaattc tgacggtacg ccgggagtaa agaacatcag
    15181 gcaacgatta agctgcacgg caaaaccaca ggcggtgcct accgggttat tgataaattc
    15241 agcactggca ggcagctccg cttgtttacg gttgctcggt gccattactc gtccacgttc
    15301 gtggaaatag cgttccatct ctttgagcca ggcttcatgc agcaccaggc cttcaccttt
    15361 tgctgtcgca gcggcgagtg cgcttaaatc atcgctggtc ggtcccagcc cgccgttaac
    15421 gatcagcaca tcggcgtgct gactacgttc gcgaagaatg gtgactaagt catcaaggtt
    15481 atcccccacc gtattgcggc gagataatgg cagcccctga tgaaagaaaa aatcggccag
    15541 ccaggcagcg ttagtgtcaa cgatttgccc gtataacact tcatccccgg tggatagcat
    15601 ttccactttt aacatggtta tctcctgctt ttgttatcga atcactataa cgcaaaggga
    15661 gataaggcgt agtaagaaac tgaatgccgc gccggaaggg atcagaaact ggcgttaacc
    15721 ccaacataca ggccgtcagc cacagcgttg tcgcggttac cgtctttacc cgacagattc
    15781 aggtagcgat aacccgcctc aatactgact ggacgcataa tggtgtaacg cgcgccagca
    15841 ttcgcttcct cataactttg aataccgctg gagagcgaat ccggagagta gtaatactcg
    15901 ccaaacaaac ggaagctgtt gccaattttc cactgcaaac cacctcctac cgctgcggca
    15961 taaccttcat cgccgtaatt cgggttggtg tacacgcctt ttccgccaac ggtcgccatt
    16021 aacggcccga gaggcagatt caaccccagc cccacgcccg cgacgtcacc gtcgtcgtcg
    16081 ttatgtgtcc agttaccgct taaagccagg cccgtcgatt cagtcccaaa accgacacca
    16141 atattggtgt attctttacc cgcctggccg ctgatgctca ttgcattgac cgatgcagaa
    16201 accaacagca ttccggccag acctgctagc gcaatttttt tcattatccc ctttcccaca
    16261 agcaattaat caaaattccc aaagctgcgg gattgtactg cctggcacag aggattcaat
    16321 gagcgaaaaa agggatctag tcacttattg taactaaata cttcttaaat aattaaacgc
    16381 cgaaaaaata aacacacttt accgatacac acaaaaataa taataagtgc agttatcatt
    16441 gcaaaagaga ttactgtcac tttcacatat ttctgagtta ccgtattctc atggtttctt
    16501 tttatttata aggaataccc ctgtgcgaca acggactatt gtatgccctt tgattcaaaa
    16561 tgatggtgct tatttgctgt gcaaaatggc cgacgatcgc ggcgttttcc ccggtcaatg
    16621 ggcgctttcg ggtggcggcg tggagtctgg cgaacgaatt gaagaggcac tacgccgcga
    16681 aattcgcgaa gaactgggag aacagctgct tttgacagaa atcacgccgt ggaccttcag
    16741 cgatgatatt cgcaccaaga catatgcaga tggtcgcaag gaagagattt atatgattta
    16801 cctgattttt gactgcgttt ctgccaaccg agaagtgaaa ataaacgaag agtttcagga
    16861 ctacgcgtgg gtaaaacctg aagatctggt gcattatgat ttaaatgttg ccacccgaaa
    16921 aacgttacgt ttgaaaggtc ttctttaaca atgatgacag ccatcactgg ctgtcagatt
    16981 gaaaatttag tggttaacga attccccatc cagataaact ttgccttttt ccacatgcat
    17041 gactaaacca tccagataat caggtttaaa tatcgcatca cgcttatttt tagcaatata
    17101 tgtcaggcaa tgattatggg tgaaaataac gatattttta tcaggcgctt tgctttgtaa
    17161 gtccttaatt gcactataaa tctcattacc gcactgaaga agtcgtttat ctaccgtcag
    17221 ctttttaccc gcggaaaacc aggtcgccga ctgaatggtc cggacggtat tactggaata
    17281 aagaccgaaa tcagagatgt cagcgctaaa agcgttgccc agttcacggg catcctgggt
    17341 acctttaacc gtaatacctg ttttatctga caagcattga ttggttgaac gatcgcaacg
    17401 ttcagcatgg cgaaacaaaa cgacaaccgg gtgctgctgt gccagtctgg ccaatgtttt
    17461 attgtcgata cgtggcaaac cattgctact ccaggcggca tgagtaccca gtccggcaat
    17521 tgcagcgagc gccagtaaaa tgatgaaata tttttttgac ttcaatgatg agcggcaaaa
    17581 agctaacacg taaactccac ctatagacaa gcgcaaccag acaattaccg tgaaattgag
    17641 cgacatttct ggcgataatt agcagttggt gtaatattaa aaatcctacg atgtcggcaa
    17701 aatgcctcaa atttttgcca aatgcaaagc ctaaataaga aaaaatataa aaatttcaat
    17761 atttacgtct aatattagtt tcttaaggtt aagttaatat tctatcctta aaatttcgct
    17821 ccaaatggca aaatatacac aacactcttt atagcaaata taagtggaca ggtattcaat
    17881 ggcggaagga aaagcaatgt cagaattttt gcctttttcg cgaccagcaa tgggcgtgga
    17941 ggaactcgct gcagttaaag aggttctcga atccggttgg atcacaaccg gtccgaagaa
    18001 tcaggcgctt gagcaagcct tttgccagtt gacgggaaat cagcatgcca tcgcggtcag
    18061 ttcagccacc gccggaatgc atatcacgct aatggcgttg gaaattggca agggcgatga
    18121 agtgattacg ccttccctga cctgggtttc aaccctcaat atgatttcct tgctgggtgc
    18181 aacgccggta atggtggatg tcgaccgcga tacgctgatg gtcacgcctg aagcgatcga
    18241 atcagccatt acgccacgca ctaaagccat cattccggtg cattatgccg gtgcgccagc
    18301 agatattgac gccattcgcg ccattggcga acgttacggc atcgcagtta tcgaagatgc
    18361 tgcccatgcc gtcggtacgt attacaaagg gcgacatatt ggcgcaaaag gtaccgctat
    18421 tttttcattt catgccatta aaaatattac ctgtgctgaa ggtggcctga ttgtaactga
    18481 taatgaaaac cttgcccgcc agctacggat gctgaaattt cacggtctgg gtgtcgatgc
    18541 ctatgacaga caaacctggg gccgtgcacc gcaggctgaa gtcttaacac cgggctataa
    18601 gtacaatctg accgatatta acgccgcgat tgccctgaca cagttagtca aattagagca
    18661 cctcaacacc cgtcggcgcg aaattgccca gcaatatcag caagcactgg cagctctccc
    18721 ctttcagcca ttaagccttc ccgcctggcc gcacgttcac gcctggcatc tgtttattat
    18781 tcgtgtcgat gaacaacgtt gtggtatcag tcgcgatgcg ttgatggaag cgttaaaaga
    18841 aagaggcatt ggtaccgggt tacatttccg cgccgctcac acacaaaaat attatcgcga
    18901 gcgttttccc acgctgtcgt taccgaatac cgaatggaat agcgaacgca tctgttcgtt
    18961 gccgctgttc ccggatatga ctaccgccga tgccgaccgc gtcatcacag cccttcagca
    19021 actcgcagga caataagcca tgtttgaaat ccaccctgtt aagaaagtct cggtggttat
    19081 tcccgtttat aacgagcagg aaagcttacc ggaattaatc aggcgcacca ccacagcctg
    19141 tgaatcgttg gggaaagagt atgagatcct gctgattgat gacggcagta gcgataattc
    19201 cgcgcatatg ctggtcgaag cctcacaagc ggagaacagc catattgtgt ctattttgat
    19261 taaccgcaat tacgggcaac attcagcgat tatggcggga ttcagtcacg ttactggcga
    19321 cttaattatt acccttgatg ccgatctcca gaatccgcca gaagaaatcc cccgcctggt
    19381 ggcaaaagcc gatgaaggtt acgacgtggt agggactgta cgccagaacc gccaggacag
    19441 ctggtttcgt aaaacagctt cgaagatgat taaccggctt attcagcgca ccactggcaa
    19501 agcgatgggt gactacggtt gtatgctgcg cgcctatcgc cgtcatattg tcgatgcgat
    19561 gttgcactgc catgaacgca gcacctttat cccgattctg gcgaatatct tcgcccgccg
    19621 tgcaattgaa attccagtac atcatgccga gcgtgagttt ggtgaatcca aatacagttt
    19681 tatgcgcctg attaatttga tgtacgacct ggtgacctgc cttaccacaa cgccgctacg
    19741 tatgctgagt ctgctcggca gcattattgc gattggaggt tttagcattg cggtgctgct
    19801 ggtgatttta cgcctgacct tcggaccaca atgggcggca gaaggcgtct ttatgctatt
    19861 tgccgtgctg tttactttta ttggcgctca gtttatcggc atgggattac tcggtgaata
    19921 tatcggcagg atctacaccg atgtccgcgc ccgcccccgc tattttgttc agcaagttat
    19981 ccgtccatcc agcaaggaaa atgaataatg aaaaccgtcg tttttgccta ccacgatatg
    20041 ggatgcctcg gtattgaagc tctgctggct gccggttacg aaattagcgc catttttacc
    20101 cataccgata atcccggtga aaaagccttt tatggttcgg tggctcatct ggcggcggaa
    20161 agagacattc cggtttatgc gccggataac gttaatcatc cgctgtgggt ggaacgcatt
    20221 gcccaactgt caccagaggt gattttctct ttttattatc gccatcttat ttgcgacgag
    20281 atttttcagc tcgctcccgc aggtgcattt aatctgcacg gttcgctgtt accaaaatat
    20341 cgtggtcgcg cgccgctgaa ctgggtgctg gtgaacggtg aaacggaaac gggcgttaca
    20401 ttgcaccgaa tggtaaacgt gccgatgctg gggccattgt ggcgcaactg cgcgttgcca
    20461 ttgcgccaga cgatatcgct attacgctgc atcataaatt gtgccatgcc gcgcgccagc
    20521 tactggaaca gacattaccc gccattaaac acggtaatat tctggaaatc gcccagcgcg
    20581 aaaacgaagc cacctgtttt ggtcgcagaa cgccggatga cagtttcctt gaatggcata
    20641 aaccggcatc cgtactgcac aacatggtac gtgccgttgc cgatccgtgg ccgggtgcct
    20701 tcagctatgt tggcaatcag aaattcaccg tctggtcgtc gcgtgttcat cctcatgcca
    20761 gcaaagcaca gccggggagc gtgatttctg ttgcgccact gctgattgcc tgtggcgatg
    20821 gcgcgctgga aatcgtcacc ggacaggcgg gcgacggcat tactatgcag ggctcgcaat
    20881 tagcgcagat gctgggcctg gtgcaaggtt cacgcttgaa tagccagcct gcctgcaccg
    20941 cccgacgccg tacccgggta ctcatcctcg gggtgaatgg ctttattggc aaccatctga
    21001 cagaacgcct gctgcgcgaa gatcattatg aagtttacgg tctggatatt ggcagcgatg
    21061 cgataagccg ttttctgaat catccacatt ttcactttgt cgaaggcgat atcagtattc
    21121 attccgaatg gattgagtat cacgtcaaaa aatgtgatgt cgtcttgccg ctggtggcga
    21181 tagccacgcc gattgaatat acccgcaacc cgctgcgcgt atttgaactc gattttgaag
    21241 agaatctgcg cattatccgc tactgcgtta agtaccgtaa gcgaatcatc ttcccgtcaa
    21301 cttcagaagt ttatgggatg tgtagcgata aatacttcga tgaggaccat tctaatttaa
    21361 tcgtcggccc ggtgaataaa ccacgctgga tttattcggt gtcaaaacaa ttacttgatc
    21421 gggtgatctg ggcctatggc gaaaaagagg gtttacagtt caccctcttc ctcccgttta
    21481 actggatggg accacgactg gataacctta atgcagcgcg aattggcagc tcccgcgcta
    21541 ttacgcaact cattctcaat ctggtagaag gttcaccaat taagctaatt gatggcggaa
    21601 aacaaaaacg ctgctttact gatatccggg acggtatcga ggcgttgtac cacattatcg
    21661 aaaatgcggg aaatcgctgc gacggcgaaa ttatcaacat tggcaatcct gagaacgaag
    21721 cgagcattga ggaactgggc gagatgctgc tggcgagctt cgaaaaacat ccgctgcgcc
    21781 atcatttccc accgtttgcg ggctttcgcg ttgtcgaaag tagctgctac tacggcaaag
    21841 gatatcagga cgtagagcat cgtaaaccga gcatccgcaa tgcccaccgc tgcctggact
    21901 gggagccgaa aattgatatg caggaaacca tcgacgaaac gctggatttc ttcctgcgca
    21961 ccgttgatct tacggataaa ccatcatgac caaagtaggc ttacgcattg atgtcgatgc
    22021 ctttcgtggc acccgtgaag gcgtgccgcg tctgctggaa atcttgagta agcataatat
    22081 tcaggccagc atttttttca gcgtcggccc ggacaatatg ggccgccatc tctggcgact
    22141 ggtgaagcca cagtttttgt ggaagatgct gcgctcaaac gcggcatcgc tttatggctg
    22201 ggatatttta ctggcaggta cggcctggcc aggtaaagag attggtcatg ccaatgccga
    22261 tatcattcgt gaagcggcta aacatcacga agtcggtctg cacgcctggg atcaccatgc
    22321 ctggcaagcc cgtagcggta actgggatcg gcaaacaatg atcgacgata ttgcacgcgg
    22381 tcttcgcact ctggaagaga ttatcggtca accggtaacc tgttctgccg ctgcgggctg
    22441 gcgtgccgac cagaaggtga tcgaagcaaa agaagcgttc catttgcgct acaacagcga
    22501 ttgtcgtggg gccataccgt tccgtccact gcttgaatca ggaaaccctg gcactgcgca
    22561 aattccggtg accttaccca cctgggatga agtgattggt cgggatgtga aagcagaaga
    22621 ttttaacggt tggttactca accgcatcct gcgagataaa ggcacgccgg tttataccat
    22681 tcatgcagaa gttgaaggct gcgcttatca gcataatttt gtggatctcc tcaaacgcgc
    22741 agctcaggaa ggcgtgacat tttgcccttt aagcgaactg ttatcagaga cgttgccgct
    22801 cggacaagtt gttcgcggaa atattgccgg acgtgaaggc tggctgggtt gccaacaaat
    22861 tgcgggtagt cgctgatgaa atcggtacgt taccttatcg gcatcttcgc gtttattgcc
    22921 tgctattacc tgttaccgat cagcacgcgt ctgctctggc aacccgatga aacgcgttat
    22981 gcggaaatca gtcgagaaat gctggcatcc ggcgactgga ttgtgcccca tctgttaggg
    23041 ctacgttatt tcgaaaaacc cattgccgga tactggatta acagcattgg gcaatggcta
    23101 tttggcgcga ataactttgg tgtgcgggca ggcgttatct ttgcgaccct gttaactgcc
    23161 gcgctggtga cctggtttac tctgcgctta tggcgcgata aacgtctggc tctactcgcc
    23221 acagtaattt atctctcatt gtttattgtc tatgccatcg gcacttatgc cgtgctcgat
    23281 ccgtttattg cattctggct ggtggcggga atgtgcagct tctggctggc aatgcaggca
    23341 cagacgtgga aaggcaaaag cgcaggattt ttactgctgg gaatcacctg cggcatgggg
    23401 gtgatgacca aaggttttct cgcccttgcc gtgccggtat taagcgtgct gccatgggta
    23461 gcgacgcaaa aacgctggaa agatctcttt atttacggct ggctggcggt tatcagttgc
    23521 gtactgacgg ttctcccctg gggactggcg atagcgcagc gggagcctga cttctggcac
    23581 tattttttct gggttgagca tattcaacgc tttgcactag atgatgccca acatagagct
    23641 ccgttctggt actacgtgcc ggtcatcatt gccggtagcc tgccgtggct gggattactc
    23701 cccggtgcac tgtacacagg ctggaaaaac cgcaagcatt ccgcaaccgt ctatttgttg
    23761 agctggacga taatgccgct gctgtttttc tccgtcgcta aaggcaaatt gcccacctat
    23821 attctttctt gctttgcacc tctggcaatg ctgttggcgc attacgcgtt gctggcagca
    23881 aaaaataatc ctctggcgct gcggattaat ggctggatta acatcgcttt tggcgtcact
    23941 ggcattattg ccacatttgt gatctccccc tggggaccaa tgaacacgcc ggtgtggcaa
    24001 accttcgaga gctataaagt cttttgtgcc tggtcgattt tttcgctatg ggcatttttc
    24061 ggctggtaca ccttaacaaa cgtcgaaaag acctggtctt tcgccgcgct ttgcccgctg
    24121 gggctggcgt tgctggtagg attttcaatt cctgacagag tcatggaagg aaaacatccg
    24181 caattttttg tcgagatgac acaagaatca cttcagccaa gccgctatat tcttactgat
    24241 agcgtcggtg ttgccgcagg tctggcatgg agcctgcaac gcgatgacat catcatgtat
    24301 cgccagacag gtgagttgaa atacggcctt aattatccgg atgcgaaagg gagatttgtc
    24361 agcggtgatg agttcgcaaa ctggcttaat caacatcgtc aggaggggat tattacactc
    24421 gtgctttcgg ttgaccgcga tgaagatatc aacagtctcg ccattccgcc cgcagatgcc
    24481 atcgttcgtc aggagcgtct ggtgctgatt cagtatcgtc ccaaatgatc tggctaacat
    24541 tagtctttgc cagcttgctt agcgttgccg ggcagttgtg tcagaaacag gcaacctgct
    24601 ttgtggcgat aaacaaacgg cgcaaacata tcgtgctgtg gctgggactg gcgctggctt
    24661 gtcttggtct tgccatgatg ctctggctgc tggtcttgca gaacgtaccg gtaggcattg
    24721 cctacccgat gttaagtctg aattttgtct gggtgacgct ggctgcagta aaactgtggc
    24781 acgaaccggt atcgccgcgt cactggtgtg gggtggcgtt cattattggt ggcattgtga
    24841 tcctcgggag tacggtgtaa tgtgcctgat atggggatta ttcagcgtca ttattgcctc
    24901 agttgcgcaa ttaagcctgg gttttgcggc aagtcatctg ccgccgatga cgcacttatg
    24961 ggattttatc gcggctctgt tagcttttgg cctggatgcc cggatactgt tactcgggct
    25021 gctgggttat ctgctttccg ttttttgttg gtataaaacg ctacataaac ttgcgctaag
    25081 caaagcctat gccttgctca gtatgagtta tgtgctggta tggattgctt caatggtact
    25141 tcccggctgg gaagggactt tttcgctgaa agcactactg ggagtagcct gtattatgag
    25201 cgggttgatg ctgattttcc tgcccacgac aaaacaacgt tactgagttt tccctgccgc
    25261 tgtacactgc cgttcccact catcggggct ataactgctt gccgagagga ctttcaccgt
    25321 atgcagcttt tcacgattaa tacaataaag cgcattctgc aaaggcgaga gtaaatcccc
    25381 cactttcacg gcgaagtcac tcttcacttc ggcgatcatt tttatcgcgc cgccagcatc
    25441 gcagagcata agtacatgtc tgttatcttg tttattgcag cacgattttt tgtgccagcc
    25501 attccattgc attatcctgt ttgctaagag ttttcacatc aatttacaga ttaacagata
    25561 aactctttat caacggcaat ggaatggcag aaatgagaag tgaaacggag ggttaattat
    25621 tgctgacgtt gcacccactc ttttagcgcc tgacgtgaaa ttttaatacc gccgtttttc
    25681 agctccggcg gtaaagtcag ccagcgcacc ggttgttgaa aacgagccag cttatctttc
    25741 acccattcgc caagatcaac ggtttgctgg tcatactcca ccaccgctac cggccgatga
    25801 ccaaactcct tgtcggcaac ggaaacgata aacacctgca aaaccgcagg atgtgcagca
    25861 ataacacgct cgacttcttc cggctgaata ccctcaccgc cactgaagaa cagattgtct
    25921 aaacgtccga caatggtcag cttgccatta tgcatctcac cgcgatcgcg cgtagcgtac
    25981 cagccttcgt cattaaccag tgaaaccagt tgcccgttac gccagtaacc ttctgccata
    26041 ctggcagccc gcagccacac ttcattatta acgattttca cttcccgacc cggcagcggc
    26101 aaaccaacgt ctgccaggcc gtcggcttct ttcgcacata ccgtggaggc aaactcggtc
    26161 agaccatagc cgcaaaagca acgaatcccc tgctcgcgcg cctgttccgt caactcgacc
    26221 gggatagccg cgccgccaag taacaccgct ttcagggaaa cggaactgcg gttaaccagc
    26281 aaacgccaga gttgtgttgg caccagtgaa gcgtgagtac agcctgccag catttgctcc
    26341 aatggctgtt tatcacgcac cgtcatccgc gcaccagcgt ataaccagcg ccacataatt
    26401 ccctgaccgg agacgtgaaa cagcggtaaa gagagcaacc agtcatcgtg atcgccaaac
    26461 ggaatcagcg ataacacacc ttgcgcactg gcaagatggg cctgataagt atggacagcg
    26521 gctttcggca aaccggtaga acccgaggtc aacgtcattg agcacagacg cgtcggctgc
    26581 cacgcagcgg catgtacgcc ttcaaccagc tgaatgtgca gcgacgttaa tgccggaaac
    26641 gtgttttccc catccggcac cagcgcaaat tgcagcgtca gattgggcag caattcttca
    26701 agcaacggtt gcggcagctg agggttcacg ggcaacaccc gcgccccgca ttgcagtaac
    26761 gccagccagg cgagcagcgt ttgcggcgta ttccacgccc gcaacatcac gccgctgccc
    26821 tcaaccaccc cctgcgccgc aaagcccgta gctaactcat cgacgcgagc acaaagctcg
    26881 cgccagttga gttgctcgtc attaagacgt aaggcgatgg cttctccccg cacttgccgc
    26941 cagtgacgcc acggccagtc agagaagatc ataacaactg ctcctgtaca tcagcgtcca
    27001 ctaacggcag tgggctcccc ggccagcgac gaacctgctg cgcctgcatc agatccagcg
    27061 tgtccagtcc cggaatggtg tctggcgtta accaggcagc aatccgcgcc agttgcgtta
    27121 gacctaagct cgactcaatg gaagaactga tcaccgccgt cagccccagc gcatgcgccg
    27181 cctgtacctg ctcgcgtact ttttccagac tgcccgtgag cgtgggtttg ataactaccg
    27241 cgcgcacgcc ctcttcagcc acaaaggcaa aatccggctc gcgcaggctt tcatcccagg
    27301 caatggcaat gccggtttca cgggcaaacg ctcgcgaatc atcgcgggtt ttgcacggct
    27361 cttcgagaaa cgcgatgcgg tggcgataat ccgggttaac atatttggca aactgctgac
    27421 ctttcagcgg tgtccaggcg cgatttgcgt caagacgcaa atgcagatcc ggaattgcct
    27481 ccagcaacag attcaccacc atgccgtcgc gcaccgcttc gtacaatccc actttgacct
    27541 tcgccacttt ctcgcccggc atatcggcaa gtttgaggat cagatcgtcc ggatcgccat
    27601 tacacagcgg tgccgcacgg tagttggctg cttgcggcaa cgtatctgtc agttctgcca
    27661 atgcacagct tacgccaaag gccacggaag gcatctgcgg tagctcgcaa tcgcctgcca
    27721 gccagttatt tacccaggca agcagcacgc tttgcgcctc ttcccagttt tcctggctga
    27781 agcccggcag tggggagatc tccccccacc cttcgcgctc gccctcacgc aggcaaacgt
    27841 acagcccgtc gcgggttttt aaccgcctgt cgcgcagaac cacccccgcg tccatgggga
    27901 tctgccagcg gtatacctgc gcgctacgca ttacggattc cgtttaaatt tgctgaaatc
    27961 aggctgacgt ttctggttga aagcgttgcg accttcctga ccttcttccg tcatgtagaa
    28021 cagcatggtg gcgttgcccg ccagctcctg cagccccgcc tgcccgtcac agtcggcgtt
    28081 cagcgcagct ttcaggcagc gcagcgccat cgggctgttt tgcagcattt cgcggcacca
    28141 acggacggtt tctttttcca gatccgccag cggtaccacg gtgttgacaa ggcccatatc
    28201 cagcgcctgt tttgcgtcgt actgacggca caggaaccag atttctcgcg cttttttctg
    28261 cccgacgata cgagccatgt aggaagcgcc ccagccgccg tcgaaggaac cgactttcgg
    28321 gccagtctga ccgaagatgg cattatctgc tgcgatagtc aggtcgcaca tcatgtgcag
    28381 aacgtgacca ccgccgatgg agtagccagc caccatcgcg acaaccggtt tcggacaggt
    28441 acggatctga cgctggaagt ccagcacgtt caggtgatgt acgccggaat catctttata
    28501 gccgccgtaa tcaccacgca ctttctggtc accaccggag cagaacgctt tatcgcctgc
    28561 gccagtcaga atgatcacgc cgatattgtc gtcataacgc gcatctgcca gtgcctggat
    28621 catctctttt accgtcagag gacggaaggc attgcgcacc tgcggacgat taatggtgat
    28681 ttttgcgata ccgtcggtgg atttttcata acgaatgtcc tcgaaacctt cggagcagtc
    28741 gtgccattca accggtgcgt aaggcattgc ttcatcagga taaatcatat tgtgtccttt
    28801 agtcagaaac gcaagatctg cgccagactt gcgattacgc cagcgggatt ttcccgatgc
    28861 gcgttatgtc cggcgcgagg aatgacatgg cagtcggcag ccagttccgc cgccagggcg
    28921 cggaatttgc tgtcacgttc accacataaa taataaaacg caaatgtgcg ggcgctaagg
    28981 ttagcacgta aatcaggctg gacggcgaga gaagtcgcct ccagcatggc ggcaagggtt
    29041 gcgccattat tgttgctgcg cagcgccacc agctcccggc gttgatcgtc attgagcgag
    29101 gcaaaaacag gctgttgata ccagtcggca aataccgccg ttaacggttc tgtgcgaaaa
    29161 cgctgcgccc attggcgatc ggaacgctga cgttccgcac gttgttcagc attttgcagc
    29221 cccggatgcc cgccttcgac aataacccca caaagccccg ccagcccctg gcaagccgcc
    29281 atcatcgcca cccgtccgcc aagcgagtac cccaccagcc agaagtcaag gatgttgtaa
    29341 ctaaccaagg ttttacgcag taagtcggtg acatcatcaa atccatcgac gctaatcgcc
    29401 gccgaaccac catgacctag gagatcaaca tacaaccgtg agtagtcggc aaacgcctcg
    29461 cccacttctt gccattcgtg gcaatcgccg gaaaaaccgt gcaaaaacac cagccagggt
    29521 aaacctagtt ttccgtgttt tgcctgcgcg tgcaggatca taaatggctt acctgcgcca
    29581 gaagttgctg gagcgtttgc gcaccatcgg tgtcgttaac caccatttca atcaccgtgg
    29641 tggttggcgt gcgccaggcg tcggcaaatg ccgtttcaag ttcctgccag ttttgcggat
    29701 gatgatattt cagctcgaac atcgcggcgg cgtgctcaaa atggacgttt tgcggcatca
    29761 gatagaaacg ctcacgctcg ctttgcggcg ttggcaacag cgagaaaatt tgcccgccgt
    29821 tgttgttcac cacaattaat accagcggcg cagaaacctg acgcaataac gccagcgcgt
    29881 tgagatcgta aagcgcggag agatcgccca caatcgccag cgtcggtttg ccgcttgccc
    29941 gctgaacgcc tgcggcggtc gaaagcagtc cgtcgatacc gctggcccct cggttgctgt
    30001 acaccgggta acctgccgga agttgcgaaa gcgcatcaat cagacgtacc accaggctgt
    30061 taccaacaaa caattgcccc tgttcaggca gatagtcgct gatgcgatgc gccagttgcg
    30121 cttcgccaaa cgcatcacgg cgggcaataa ccgcctgcat tgcctgttcc gccaggcgcg
    30181 ggatttcaac gcaccagggc tggcgttttt ctgccggatg cagctccagc cagtcggcaa
    30241 tattggcaat taagcgacgt ccgcgatggt gtgccggatc aagtcgccct tcaatgtcat
    30301 caacaatcca gtactcttct ggttcacagc ttgcctgcca ttgcaggagc cgtttccccg
    30361 tcaggctact tcccagttgc accacaattt gcgcctgctg cagctcgctg gtcgctttgg
    30421 cattgcctaa ccaaagatcg gcacacggca gcggctgccc ggtttgtgac agcacatcgc
    30481 caatcagcgg ccagccaaga gtttgcgccc acagggcaac ttttttgccc tcttccgcac
    30541 tcatgcgccc ggcaaccacc acgccgcgct tttgtcgcca gaagaaccag tcgcgctgtt
    30601 tttcactttc cagacgaggc gcttcacgca gccacggttt gtcgtcctgc caccagtcac
    30661 ccagacgctg ttgccagcta agcccggtat cgtccatttc gccatacagc ggttcagcaa
    30721 acgggcagtt gatatggact ccccccgcat gaagcgtacc gagagcgtgg tcgatggtag
    30781 aaaccagcca acgtgcgggg atatcctggg tcgggcgcgg caatgaaata ctgtgcgtgg
    30841 ggtgagaggc gaacattccc ggctggcgaa ttgcctgatt cgcgccgcag tcaattagct
    30901 ccggcgggcg atcggcggtt aagagaatca gtttttctcc ggttaacccg gcttcaatca
    30961 gtgccggata gagatttgcc accgccgtgc cggaggtcac aatcaccgcc accggctgct
    31021 tgctgacttt cgccagcccc agcgccagat gccccaaccc acgctcatcg aaatgggtgt
    31081 ggtgaatgaa tgcggaattc tccgccgccg ctaacgttaa cggtgtagaa cgcgagcctg
    31141 gggcgataca gatgtgtctg acgccgtgac gcgttaatgc ttccagaatg accgccgccc
    31201 agcgtcggtt aaatgcgctt actgacatga gtttgtccgg tatcaatatt gcggctaagt
    31261 ataaggagct acaaaaatag attattgata tgaatcggta atgatgcgac tcattactat
    31321 tccatttgta ataaagtacg cagccctgcc gctttgttgt cgatttcctg ccactcttgc
    31381 tcggggtcgg aaccacggac aatgcccgcg ccagcatata atcgcacgac attgccgcta
    31441 atttttgctg agcgcaggga aacgcagaat tcgctttgtt gtaatgagag atagcccgca
    31501 gaaccggcgt accattctcg ggtgaacggt tcgtgacggg cgataaactg tcgcgccaga
    31561 tcgcgcggta agccagcaac tgctgccgtt ggctgcaact gatgtaaaca gatcacatca
    31621 tccgctttgt tgagtgaagt ccagatacag cggcgaagat gctgcacttt acgcagacgc
    31681 agtacctgcg gcggtaaaac atccagcgtc tgggtatcgg cctgtaatcg ttggcagata
    31741 tcttccacca ccagcatgtt ctcgcgctgg tttttatcat ccgccatcag ccactctcct
    31801 aactgctgcg cctgcttatc atcaggatga tttgctactg ttcccgccag cgcttcagta
    31861 cgcagcgctt tgtcacgccg ccgccataac cgttccggtg aagagccaag aaaagcattt
    31921 tcgccatcaa aggccatgta aaaatggtag caattcagat tcagtcgacg actggcagcc
    31981 atcatcgccg ccgcgttgac cggacttgcg aaatgcaggt cagttgcccg agcgagcacc
    32041 actttgtcga gctcaccttc ggcgatggtt ttcgttgcca gttcgattaa ttgcgtccag
    32101 cccgttttgt ccggccagtg ttgttctcgc gtggtggtta aatgtaaccc aggcaagggc
    32161 ttgatactca ccagtgtggc gataaattct tttgcctgaa tcgcatcgtg ctgaagggag
    32221 ctttcgctga atagcgtcag ccgcagcgtg gctttaccgc cacagcgtcg ccattccagg
    32281 cggggtaaaa gtaaattgcc ctgcgacggg tcaaaagcat tcagccccca aatgcgtaag
    32341 tcagcgtgtt ccgggtgctg gcgaagaaaa cgttgtgcct ggtccaacga cgtaaaacgg
    32401 gtaatcgcgc ccaggacggc agcttcttca tcaccattac gttgttgcca gtagaattgc
    32461 gggtacgttt gctgactggc cagccagctc aaggcatcaa aagcgtcttt gagagggaaa
    32521 ggaatatcga taacccgaat gccgggtgtc gccggaatct cttgcgacaa atggcgcagt
    32581 agattttcca gcgccgtagt aagtgattgc acgcggacct ctccctgtta aaagcctcac
    32641 attatacggg gtactacaaa aaaaatgcag taccccggtg tagggaggtt ttaacggcgt
    32701 gccagcaaca gtcctagtac cagcccgacg gccgcgccca caccaattcc ttgccagggt
    32761 ttttcgtgga cgtagtcatc agcacgataa acagcctgct tcgcccgata gtaataactg
    32821 tctgaagcct ggctaacccg ttttttcaca tcatccagcg ctttttctgc atgcgctttc
    32881 agctcaacat atttctgatc ggcgggatcg ccagaggagc ggagcacctc ttccagtgtt
    32941 tcactaagca gcgtcaggtc gtcatcgata cgtgtatcac caaactgatt agacattctc
    33001 gttctccatt tgcgtaaaac ctgtcactaa ctatagacaa gagttgttga ttacgcctga
    33061 attacctcgc gtgccatccc aatgtgtggg ataccatctt cttcatagac ctccgtcaca
    33121 gggataaaac cgaaactctg gtagaagttt tgcagatgcg cctgcgcccc gaggtaaaca
    33181 ggcttatcag gccagtgatg cgtacatgtt tcgagtgttt tactcatcag ctgctgcccc
    33241 actttttcac cacgcaacgc ttcactgaca atcacccgac ctataacgac cggctcaaga
    33301 tcatcatcac ttttcagaat cctcgcatac gccaccagtt catcattttt ccaaccgaga
    33361 atatggcgat tatcccccgt caaatcatca ccatcgatat cctgataagg gcaattctgc
    33421 tcgacaacaa acaccgcgca acgcagttgt aataaggcat aaagctggga aaccgataat
    33481 tcagagtgat gcagatcttg ccattcaatc ataatgctct ccttttagat gtatgcctta
    33541 tactagccgg aatatttttt gaaacgtgat gaactcatgg aattaatttt tttaggtact
    33601 tcagccggtg tgccaacccg cacgcgcaat gtcacggcaa tattgcttaa cctgcaacat
    33661 ccgacccaga gcggactttg gttgtttgac tgcggtgaag gcacccagca tcagctactg
    33721 cataccgcct ttaaccctgg aaaactggac aagattttta tcagtcacct tcatggcgat
    33781 catctttttg gtttacccgg cttgctgtgc agtcgttcta tgtcaggcat tatccaaccc
    33841 ttaacgattt atggtccgca aggtatccgt gaatttgtgg aaaccgcgct gcggattagc
    33901 ggctcatgga ccgattatcc gctggaaatt gtcgaaattg gcgctggcga aattctcgat
    33961 gatggcctgc gcaaagtaac cgcttatccg ctggaacacc cactggaatg ttatggctat
    34021 cgtattgaag aacatgataa accgggtgca ttaaatgccc aggcattaaa agctgctggc
    34081 gtgccgcctg gcccactgtt tcaggaatta aaagcgggca aaacaatcac gctggaagat
    34141 ggaaggcaga ttaacggcgc agattactta gctgctccag tgccaggtaa agcgctcgct
    34201 attttcggcg ataccggccc ctgcgatgcc gcacttgacc tggctaaagg tgtcgatgtc
    34261 atggtgcacg aagcgacgct ggatataacc atggaagcca aagccaatag tcgcggccat
    34321 agctctacac gccaggctgc gacactagcc cgtgaggctg gagtcggcaa gctaatcatt
    34381 acccacgtca gctcgcgcta tgatgacaaa ggttgtcaac acctgttacg tgaatgcagg
    34441 tcaattttcc cggcgactga actggcgaat gatttcaccg tgtttaacgt ttaactctgc
    34501 ttattattaa aaacagggtg aaacttgccc tgttatagca acccgcgcta catatatcca
    34561 cccacacagg catattgagc gatttttgat atttattcat ctccttacgc actatattca
    34621 gagcaataag ttgcctcttt tcatcttgat tcagcacaac ctttgcaact aaaaggagcc
    34681 catgatggtt acagttgtca gcaattattg tcaattatct caaacccaac tcagtcagac
    34741 atttgcagaa aaatttactg tgaccgagga attactgcag tctttaaaaa aaacagcgtt
    34801 atccggagat gaagaaagta ttgagttact gcataatatt gcgttaggtt atgatgaatt
    34861 tggaaaaaaa gctgggtaat gctgccaact tactgattta gtg
//
LOCUS       DADLTY010000020        34788 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.020, whole genome shotgun sequence.
ACCESSION   DADLTY010000020 DADLTY010000000
VERSION     DADLTY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.020"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..1237
                     /locus_tag="JN334_000828"
     CDS             <1..1237
                     /locus_tag="JN334_000828"
                     /inference="COORDINATES: protein motif:HMM:NF028277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7437072.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTF"
     gene            1213..2097
                     /locus_tag="JN334_000829"
     CDS             1213..2097
                     /locus_tag="JN334_000829"
                     /inference="COORDINATES: protein motif:HMM:NF036416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7437073.1"
                     /translation="MAVIAYLLNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNV
                     YATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQ
                     DEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIING
                     AGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFT
                     LEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQL
                     KQPKLRGNVQTAQREVAP"
     gene            2550..3182
                     /locus_tag="JN334_000830"
     CDS             2550..3182
                     /locus_tag="JN334_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HAY7437074.1"
                     /translation="MKNLPIGGVWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFP
                     EFAHATPRVQLDLHPTGSVNGSNYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAA
                     GRRDGYFSIYRQGGTTTDEGERIDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRP
                     VVLPGIRYAVMCVPTPLTLAVDKFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3270..4568)
                     /locus_tag="JN334_000831"
     CDS             complement(3270..4568)
                     /locus_tag="JN334_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7437075.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5053..6465
                     /gene="uxaC"
                     /locus_tag="JN334_000832"
     CDS             5053..6465
                     /gene="uxaC"
                     /locus_tag="JN334_000832"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAY7437076.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6480..7967
                     /locus_tag="JN334_000833"
     CDS             6480..7967
                     /locus_tag="JN334_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAY7437077.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8050..8601
                     /locus_tag="JN334_000834"
     CDS             8050..8601
                     /locus_tag="JN334_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HAY7437078.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8606..9850)
                     /gene="sstT"
                     /locus_tag="JN334_000835"
     CDS             complement(8606..9850)
                     /gene="sstT"
                     /locus_tag="JN334_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAY7437079.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNEPTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10446..11411)
                     /gene="alx"
                     /locus_tag="JN334_000836"
     CDS             complement(10446..11411)
                     /gene="alx"
                     /locus_tag="JN334_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HAY7437080.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11682..12698)
                     /locus_tag="JN334_000837"
     CDS             complement(11682..12698)
                     /locus_tag="JN334_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY7437081.1"
                     /translation="MEYPRVMIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQ
                     AQHFANDFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPL
                     ASNLAEVDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSS
                     RYQRYLDGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAH
                     GVVVMDYGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDL
                     TQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL
                     YLPK"
     gene            complement(12759..13451)
                     /locus_tag="JN334_000838"
     CDS             complement(12759..13451)
                     /locus_tag="JN334_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HAY7437082.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13528..14031)
                     /locus_tag="JN334_000839"
     CDS             complement(13528..14031)
                     /locus_tag="JN334_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7437083.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14116..15252
                     /gene="rlmG"
                     /locus_tag="JN334_000840"
     CDS             14116..15252
                     /gene="rlmG"
                     /locus_tag="JN334_000840"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HAY7437084.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15537..15851
                     /locus_tag="JN334_000841"
     CDS             15537..15851
                     /locus_tag="JN334_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase HigB"
                     /protein_id="HAY7437085.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15848..16264
                     /gene="higA"
                     /locus_tag="JN334_000842"
     CDS             15848..16264
                     /gene="higA"
                     /locus_tag="JN334_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HAY7437086.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16309..18327)
                     /locus_tag="JN334_000843"
     CDS             complement(16309..18327)
                     /locus_tag="JN334_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HAY7437087.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18553..20904)
                     /gene="ygjK"
                     /locus_tag="JN334_000844"
     CDS             complement(18553..20904)
                     /gene="ygjK"
                     /locus_tag="JN334_000844"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAY7437088.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20921..21992)
                     /gene="ygjJ"
                     /locus_tag="JN334_000845"
                     /pseudo
     CDS             complement(20921..21992)
                     /gene="ygjJ"
                     /locus_tag="JN334_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(22126..23559)
                     /locus_tag="JN334_000846"
     CDS             complement(22126..23559)
                     /locus_tag="JN334_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7437089.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23622..24071)
                     /locus_tag="JN334_000847"
     CDS             complement(23622..24071)
                     /locus_tag="JN334_000847"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HAY7437090.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24068..27154)
                     /gene="ebgA"
                     /locus_tag="JN334_000848"
     CDS             complement(24068..27154)
                     /gene="ebgA"
                     /locus_tag="JN334_000848"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HAY7437091.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="JN334_000849"
     CDS             complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="JN334_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HAY7437092.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28539..28870
                     /locus_tag="JN334_000850"
                     /pseudo
     CDS             28539..28870
                     /locus_tag="JN334_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28912..30402)
                     /gene="ygjG"
                     /locus_tag="JN334_000851"
     CDS             complement(28912..30402)
                     /gene="ygjG"
                     /locus_tag="JN334_000851"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HAY7437093.1"
                     /translation="MITAFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGVFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNL
                     PAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRV
                     LVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            30709..32229
                     /locus_tag="JN334_000852"
                     /pseudo
     CDS             30709..32229
                     /locus_tag="JN334_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(32383..33006)
                     /locus_tag="JN334_000853"
     CDS             complement(32383..33006)
                     /locus_tag="JN334_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY7437094.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33283..34028
                     /gene="nfeF"
                     /locus_tag="JN334_000854"
                     /pseudo
     CDS             33283..34028
                     /gene="nfeF"
                     /locus_tag="JN334_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34082..34157)
                     /locus_tag="JN334_000855"
     tRNA            complement(34082..34157)
                     /locus_tag="JN334_000855"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34122..34124),aa:Ile,seq:cat)
     gene            34282..34788
                     /gene="mug"
                     /locus_tag="JN334_000856"
                     /pseudo
     CDS             34282..34788
                     /gene="mug"
                     /locus_tag="JN334_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8482 a         8946 c         9144 g         8216 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttaaacg attcattcag ccataaagaa
     1261 cttggcgtaa ccgtcggagg cctgaacacc gataccataa acagcgcggt taacgggcgt
     1321 tgggatgggc aatacggaaa tgtctacgct accgtatctg atagttatga ccgtaagaat
     1381 catgatcatc tttcggcctt tacgggcact tatagctcta cgctggctgt cagtcgctat
     1441 ggcgttaatt tgggagccag tggtacagac gatttgctgg gtgcggtact ggtggatgtg
     1501 aaaggcttct ctgaacagga tgaagagagt caggatctgc aactcgaagc gcgggtggca
     1561 ggcagccgaa ctttgcagct tggtcaaagt gacagtgtgt tgttccctta tcctggattt
     1621 cagtctggtt ttgttgaggt taacgacagt agccagggca atcagcaggg gacaacaaac
     1681 atcattaacg gtgcagggaa tcgtgaatta atgttgttgc ctggcaagct gcgctatcgc
     1741 gaagtgtctg ccagctttaa ctacaactat atcggtcgtt tattactgcc ggcagcggtg
     1801 aaaaaattcc cgattgtggg gctgaacagc gccatgttac tggtagctga agatggcgga
     1861 tttacacttg aaattaacgg cagcgaaaaa gagctctatc tgctttccgg gcagcaattc
     1921 cttaagtgtc cactgagtgt tgtaaagaaa cgcgccagca ttcgttacag cggagatgtt
     1981 acttgtagtg tggtgactta ttcacaatta ccggagtcga ttcaggttca ggcacagttg
     2041 aaacagccta aattacgtgg aaacgttcag acggcgcaaa gggaggttgc accatgagaa
     2101 atcgattgat tgcggcgata ttgggcttgt ttggcacgct cactggcgtt caggcagctc
     2161 ctgacgtgac cagtgaaatt acgtatgatt tggcatctgg tagagcggat tattacttct
     2221 ggaaggatga agcgtcggca ggaaataatg gatatatgtg gtatgaatgt tcgtatcctg
     2281 acctccaaca aacctgtaca gctaatggaa atatatcgac agtacaaatc tatttaactg
     2341 aacaacgcag tgggatgcgt tggccggtaa aactcaaagg atttaaaaca gccattgtaa
     2401 gtagcgatga agcgccgcta ggatgcaagg ggggcaaagg gcttcagacg aatcttaagg
     2461 attctaatag atcttcatgt acagaagatg gtcaacatta ttatatatac gatacaaagt
     2521 ttcttacgct ctaccttgag cagacagaga tgaagaattt gccgattggt ggcgtctgga
     2581 aggggaaagt taaattacat tcgaacagcc cggcccagga ctatttcgca aatattaccc
     2641 tgaatacgct cgaccccaac catattgacg tgttcttccc ggagttcgcc cacgccacgc
     2701 caagggtgca gttagacttg catccaacag gaagcgttaa cggcagcaac tacgcgcaag
     2761 atctgaccat gttggacatg tgcctgtacg atggttttaa cggtaatgcc atcagttatg
     2821 aaatcatgct caaagatgaa gggcgaccag ctgcagggcg cagagacggt tacttctcta
     2881 tctatcgtca gggagggacc accaccgacg agggagaacg cattgattac cgggtcaaaa
     2941 tgtacaaccc ggaaaccggt gggcaaattg atgtgcgcaa taatgaaaat atggtctgga
     3001 acagcattaa cctgaaacgt gtgcgtccgg tggtactgcc cggtattcgc tatgccgtga
     3061 tgtgtgtgcc gacacccttg actctggcag tcgataaatt cagcgtgatg gataaacagg
     3121 ccgggtacta catgggcaaa ttgtcagtaa tctttacgcc ttccttgcca accatcaatt
     3181 aaccctgcaa accggacaaa cgcttttttc tatataaacg acgaagccgc ctgataaggc
     3241 ggcttcgtcg ttataactta cgttaaacgt taatgttgcg gtgcgggatc gttatgtgtt
     3301 tcctgcgcca cctcaatcgc cggtttgttc tgcaatacgg tccagataac cagcgcaccc
     3361 agcaggtcaa atactgccag aactgcgaac agcgggctga agccgatggt gtcagccagt
     3421 gcaccgacaa ccagcgcaaa cagcgtactt gccagccatg cggacatccc ggttaaaccg
     3481 ttcgccgtcg ccacttcgtt acgaccgaac acgtcagaag agagcgtaat cagcgcgcca
     3541 gacagtgcct ggtgggcaaa accaccgata cacagcagca taattgcgac atacgggttg
     3601 gtgaacagac cgatcatacc cgggccaatc atcagcacag cacccagcgt tacgaccatc
     3661 ttacgggaaa cgatcaggtt cacaccaaac caacgctgga acagcggcgg caggtaacca
     3721 ccgaggatac aaccgaggtc agcaaacagc atcggcatcc aggcgaacat cgcgatctct
     3781 ttcaggttaa agccgtaaac tttaaacatg aacagcggga tccacgcgtt aaaagtaccc
     3841 caggccggtt ctgcaaggaa acgcggcagt gcgatacccc agaactgacg gttacgcagg
     3901 atctgaccaa cggacatttt cttcgccgtg ctcacctggt gctgggcttc ctgaccatta
     3961 ataatatagt cgcgttcttc atcggtcaga tgcttctggt cgcgcggatg tttatagaaa
     4021 atcagccatg ccatcgccca gataaagctc aatgcaccgg agatgataaa tgccatctgc
     4081 cagctgtgca tcacgattgc ccataccacc agcggcggcg caatcatcgc accaatcgaa
     4141 gaacctacgt taaagtagcc tactgcgatg gaacgctctt tcgccgggaa ccattcggag
     4201 ctggctttca gacccgccgg gatcatcgct gcttccgcgg caccgaccgc accacgagca
     4261 acagctaggc caccccagct acctgccagc gcggttgcac cacagaacac ggcccacagc
     4321 acagcaaaca ttgcataacc gattttcgta cccagcacat ccagtacata acctgctacc
     4381 ggttgcatga ccgtataagc agcagaatag gctgcgatga tataggaata ctgttgggtg
     4441 gagatgttta actcttccat cagagttggc gcagctgccg ccacagtgtt acgcgtcagg
     4501 taaccaagca cggtgccgag cgtcaccagt gcgatcatat accaacgtaa ccctttaatt
     4561 ttacgcatgt aaaacctcat cgttttgtta tatccgcagg aagcgcggcc atcatcccgc
     4621 cagaggctga agatgttagt tacgaccggg taaccgaaaa aaaaaaccgg cacgccccga
     4681 acgttgccat gaaagtttcg tgctaattcg gcttccgtac cggttaaccc gattcatcta
     4741 acaaatgaat gaatcggcaa tttgcattcc gtcgacttat gatttgcgac ggcagaaaga
     4801 taacttgtca tacaacctta aaaggtgaga gccatcacaa atgtgcgaat atttgtaggg
     4861 acattacctg acgacagcga ggccagtact ggcgcggcct gcagcgagat ttaccacttt
     4921 gagagtaatt tttttaacta cgtttattga tctaactcac gaaaatatct tcggactctg
     4981 gaaattggtg tgataacttt gtcggcatcg caccataagc aagctagctc actcgttgag
     5041 aggaagacga aaatgactcc gtttatgact gaagatttcc tgttagatac cgaatttgcc
     5101 cgccgtctgt atcacgacta cgcaaaagac cagccgattt tcgattacca ttgccatttg
     5161 ccgccgcagc agattgcgga agactatcgt tttaaaaacc tgtatgacat ctggttgaag
     5221 ggcgatcact acaaatggcg cgctatgcgt accaacggtg tggctgagcg tctgtgtact
     5281 ggtgatgcgt ctgaccgtga aaaatttgac gcctgggcgg cgactgttcc gcacactatc
     5341 ggcaacccgt tataccactg gacgcacctc gaactgcgtc gtccgttcgg tatcactggc
     5401 aaattgcttt ctccgtcaac tgccgatgaa atctggaacg aatgtaacga actgctggcg
     5461 caggataact tctccgcgcg cggcatcatg cagcagatga acgtgaaaat ggtcggcacc
     5521 accgatgacc cgatcgattc tctggagcat cacgcagaga tcgccaaaga cggctctttc
     5581 accatcaaag tgctgccgag ctggcgtccg gacaaagcct tcaatatcga acaggcgacc
     5641 tttaacgact acatggcgaa gctgggcgaa gtttccgata ccgacattcg ccgctttgct
     5701 gacctgcaaa ctgccttgac caaacgtctg gatcacttcg ccgctcacgg ctgtaaagtg
     5761 tctgaccatg cgctggacgt ggtgatgttt gctgaagcga acgaagcgga actggacagc
     5821 attctggcgc gccgtctggc tggcgaaacc ctgagcgagc acgaagtggc acagttcaaa
     5881 actgcggtac tggtgttcct tggtgccgaa tatgcacgtc gcggctgggt acagcagtac
     5941 cacattggcg cgctgcgtaa taacaacctg cgtcagttca aactgctggg gccggatgta
     6001 ggctttgact ccatcaacga ccgtccgatg gcggaagagt tgtctaagct gctgagcaag
     6061 cagaacgaag aaaacctgct gccgaaaacc attctctact gcctgaaccc gcgcgataac
     6121 gaagtgctgg gcaccatgat cggtaacttc cagggcgaag gtatgccggg caaaatgcag
     6181 ttcggttccg gctggtggtt taacgatcag aaagacggta tggaacgtca gatgacccaa
     6241 ctggcgcagc tcggtctgct gagccgcttt gtcggtatgc tgactgacag ccgtagcttc
     6301 ctgtcataca cccgtcacga atacttccgc cgcattctgt gccagatgat cggtcgctgg
     6361 gtggaagcgg gcgaagcacc ggcggacatc aacctgctgg gcgagatggt gaaaaatatt
     6421 tgctttaaca atgcgcgtga ctacttcgcc attgaactga actaaggtct ggggttgata
     6481 tgcaatacat caagatccat gcgctggata acgtcgcggt cgctttagca gatttggctg
     6541 aaggcacaga agtcagtgtc gataaccaga ctgttacgct gcgccaggat gttgctcgtg
     6601 gacataaatt tgcgttaacg gatatcgcaa aaggggccaa cgtcatcaaa tatggcctgc
     6661 cgattggtta tgcattggcg gatattgcgg cgggggaaca cgtccacgcc cacaatacgc
     6721 gcacgaatct gagcgatctg gatcagtatc gctatcaact tgattttcag gatctgcctg
     6781 cgcaagcggc agatcctgaa gtgcagatct atcgtcgcgc taacggcgat gtcggggtgc
     6841 gtaacgagct gtggatcctg ccaaccgtgg gctgtgtcaa cggcatcgcg cggcaaatcc
     6901 agaaccgttt cctgaaagag accaacaacg ccgaaggtac cgacggcgtg ttcctcttca
     6961 gccacaccta tggctgctca caactgggcg acgatcacat caacacccgc accatgctgc
     7021 aaaacatggt gcgccacccg aacgcgggcg cagtgctggt gattggtctg ggctgtgaaa
     7081 acaaccaggt tgccgcattc cgtgaaacgt tgggcgatat cgatcctgaa cgcgttcatt
     7141 tcatgatctg ccaacagcag gatgatgaga tcgaagccgg gatcgagcat ttgcatcagc
     7201 tgtataacgt gatgcgcaac gataaacgcg agccaggcaa actcagcgaa ctgaagtttg
     7261 gtctggagtg cggtggttct gacggtcttt ctggtattac tgctaacccg atgctggggc
     7321 gtttctctga ctacgtgatt gctaacggcg gtactaccgt actgaccgaa gtgccggaga
     7381 tgtttggcgc agagcagttg ctgatggacc attgccgcga cgaagcaacg tttgaaaaac
     7441 tggtcaccat ggtcaacgac ttcaaacagt actttattgc ccatgatcag ccgatctacg
     7501 aaaacccatc accggggaac aaagcgggcg gtatcaccac gctggaagac aaatcacttg
     7561 gctgtaccca gaaagcgggt tccagtgtcg tggttgacgt gctgcgttac ggcgagcgtc
     7621 tgaaaacgcc agggctgaac ttgttaagtg cgccgggtaa cgatgccgta gcgaccagcg
     7681 ccctggcggg tgcgggctgc catatggtgc tgttcagtac tggtcgcggc acgccatacg
     7741 gtggatttgt gccgacggtg aaaatcgcca ccaacagtga actggcggcg aagaaaaaac
     7801 actggatcga ctttgacgcg ggtcagctga tccacggtaa agcgatgccg cagttgctgg
     7861 aagaatttat cgacaccatc gttgagtttg ccaacggtaa gcaaacctgc aacgagcgta
     7921 acgacttccg cgaactggcg atcttcaaaa gcggcgtaac gctataaagt cgaaatgagt
     7981 tgttaataaa aaacggcgtt tcataatgtg aagcgccgtt tgcttttcca tacctgctaa
     8041 ggatttatca tgaccgcgta ttggctggcc cagggcgtgg gtgtcatcgc ctttctgatt
     8101 ggtatcacaa catttttcaa tcgtgacgaa cgtcgcttca aaaagcagct ttcggtctat
     8161 agcgccgtta ttggcgtaca tttttttctt ctgggcacct atcccgctgg tgccagtgcc
     8221 atccttaatg ccattcgtac attgattacc ttacgcacgc gcagcttatg ggtaatggcg
     8281 atttttattg tgctgactgg cggaattggc ctcgcgaagt tccatcatcc tgtcgaacta
     8341 ttgccggtta tcggtacgat tgtcagtacc tgggcgctgt tccgctgtaa agggctcacc
     8401 atgcgctgcg tgatgtggtt ttcaacgtgt tgctgggtga ttcacaactt ctgggcgggg
     8461 tcgataggcg gcacgatgat tgagggtagt tttctgctta tgaatggcct gaatatcatt
     8521 cgtttctggc ggatgcagaa aaggggaatt gatccgttta aagtagagaa aacccctccc
     8581 gccatagacg aaaggggtta aacaattaat tacgcagggc gctatttgcc agacgatcgt
     8641 cttccgcctg gcaagctgcc gcagtgaaca gcacgtcagt tgaagagttc agcgcggttt
     8701 cgcaagagtc ctgcaatacg ccgataataa agccgacggc aaccacctgc atggcgatat
     8761 cgttcgaaat accgaacata ttacaggcca gtgggatcag cagcaaagaa ccccccgcca
     8821 cgccggatgc gccacaggca cacagagaag ccactacgct caacagcagc gctgtgggca
     8881 gatcgaccgg aatacccagc gtattaaccg cagccagcgt caacacggta atggtgattg
     8941 ctgcgcccgc catattgatg gtggctccca gcggaataga aactgaatag gtatcgcgat
     9001 ccagattcag cttttcacac agcgccatat tcaccggaat gttcgctgca gagctgcggg
     9061 tgaagaaggc atacacaccg ctttcgcgca ggcacagcag caccagcggg aacgggttac
     9121 gacgaatttt ccaccacacc agcaatgggt taaccaccag cgccaccagt aacatacagc
     9181 caaccagcac gaccagcagt tgcgcgtagc cccacagtgt ggagaaaccg gtggttgcca
     9241 gggtagaaga aaccagccca aaaataccga acggtgcgaa gcgaatgacc agtttcacca
     9301 taaaggtaac ggcattcgac atatcattga ccaggttttt ggtgggctcg ttaccgtgac
     9361 gcagtgcgaa gccgaggccg atcgcccaca ccagaatccc gatgtagtta cctttcagca
     9421 gggcgtcgat ggggttggaa accatgctca ttaccagtcc gcgcatcact tcgacaatgc
     9481 ctgacggcgg cgaaatatca cccgcgctac tggacaagtg cagggtagaa gggaaggcaa
     9541 agctgaagac tactgcggcc agagctgcag agaaggtgcc cagcagatag aggaacaaaa
     9601 taggacggat attggttttc tgcccgtgct ggtggttagc aatagatgcc attaccagca
     9661 tcaacaccag gatgggggca acagctttca gtgcgccgac gaacaaagta cctaacagac
     9721 caacagcttc cgccgcgggt tttgagatcc atgccagaag aatccccaga acaaggccga
     9781 ccaggatttg ttttaccagg ctgccatgag ccagacgccg gaatagcccc ggtgaacgtt
     9841 gcgtagtcat ttttcgatcc tttcattgtg ttgtcgcaca tccctggtgc gttctgtcga
     9901 cgcatctttc aggatgtaac aaagtgtttg caaggtcgag tataaggaaa gttacagctt
     9961 cgggaagcga aaaatgctgg attttaaaga gaggtcatgt tttgcaactt aatggttgca
    10021 tttatttaac ataaacagag ggaaggactg gctactaagg ctgattagat aacatatggg
    10081 caaaactatt tggaatatga gaacggttgc gctcctttaa cgtgttatgc catataaagt
    10141 cactggcgca gggagcgcgc agggggcggc caatcgccgc cgccccctgc acccccaggc
    10201 tctggcgaac aaaatcgccg ctgcgcggtt ccctcagcct atcccttgcg gctaccgggt
    10261 cgggcgcaag gtaacgtccc tgtaagatgc gccctcgccc cggcctccag ggaacgcttc
    10321 ggcgattttg acgccaccaa cacccgtgcg gtctttttgt aaagagctat aagccgatta
    10381 cataattaat agctcacctt tgtctggaat gagttatctc taggaatggc ttaatataca
    10441 gcgaatcatt ctccccgctg cttatcatgc cgataattca cccaggcgtt gataataaac
    10501 gtcatcacca gaatgccaaa caccacgccc agcgagacgg cgattggaat atggtagaag
    10561 tcgacaatca gcatcttgat accgataaac accagaatca ccgccaggcc atatttgagc
    10621 atcgagaaac gctctgctac gcccgccagc aggaaataca tcgcacgcag gccgaggatt
    10681 gcaaacaggt ttgaggtcag cacaatgaac gggtcagtgg tcacggcgaa gatagccgga
    10741 atgctatcca cggcaaaaat cacgtcgctc aattccacca gaatcagtac cagcatcagc
    10801 ggtgtggcgt acagcaatcc attcttacgc acaaagaaat gctcgttgtc gatggtgtcg
    10861 gtcatgcgca aatgaccgcg tagccagcgc accagcggct tgtcgccaat gcctgattca
    10921 tcttcatggg cgagcgccat cttcacgccg gtaaacagca ggaaggcacc gaagatatac
    10981 aggatccagt cgaactgcga aatcagccag ctgcctgtga agatcatgat ggtacgcaga
    11041 acaatcgccc cgagcacgcc atacaccagc acgcgacgtt gtaatgccgc cggaacggag
    11101 aaatagctga acaacatcag ccagacaaag acgttatcga ccgccagcga tttctcaatc
    11161 agataacccg tgagaaaggc cagggcctgt ggatcggcaa cggcgcgacc ttcggtttgc
    11221 accagatacc accagaaagc ggcgttaaac agtaacgaca gcgtcaccca gaccagcgac
    11281 caggccgcag cctgtttcat ggtcatggca tgtgccccac gacgcccctg caacaacagg
    11341 tcgatagcca gcataatggc gacaacaaca gcgaatccgc cccatagcaa cggcgtgccg
    11401 acagtattca tagaagttcc ttacacataa aaaaacggcc aacgtcggaa gacgtcagcc
    11461 gctgcttttt atgcatagac ctcgccttcc ggcaaggtct cacttacaac aaaaaaggaa
    11521 tacgtctgcg tattcctttc gggttgcccg gtgaccggat gtggtttttc actcatcgta
    11581 atgacgatcg accggcaatg aagttactcc cctttgcagg taacaaagta tgacagacca
    11641 ttcagtcagt caatggtctg ttacatacct tttacactgt tttactttgg caagtatagt
    11701 tttacgctat ctgccggaaa aatcacccca gtttggcggc ggatctcggt cagcagtttg
    11761 gctgtgatgc gactgaccgc cagccctgga tgatccacca gatgctcatc caccagggta
    11821 gcgaacagct ctgcttcgta gagcatggta ttaatatgct gcggctgggt gagatcctgc
    11881 atctggctgc cacgcggcac aaagcagact ttctggcatt cagacagttt ttcaatcacc
    11941 agcgatcccg cttcgccctg aatctcgctt cccaggacag aatcactgac ctttgagtgc
    12001 tgcaaggtga cgctgaaatc accgtaatcc atcaccacca cgccgtgggc gtcaacgccg
    12061 cttgccagca aactcgccgt tgcctgcacg cttttcggct caccaaataa cgccaccgcc
    12121 gacgccaggc agtaaaagcc gatatccata attgaaccgt tagagaatgc cggattaaag
    12181 gtgttgggat tctcaccgtc caggtaacgt tgataccgcg aggaatactg gcaatagttg
    12241 aaaaagactt tacgcagttt gcctgctttc ggcagcgcct ggcgcaacaa atgaaagttc
    12301 ggcaggcagg cggttttaaa tgcctcaaac agcaccacct gattttcgcg cgcacaggca
    12361 atggcggcat ccacttccgc cagattcgac gccagcggtt tttcgcaaat cacatgaatt
    12421 ttatggctaa ggaaaagttg tgtctgggaa aagtgcaggg aattcgggct ggcaatatac
    12481 accgcgtcaa tggcatcgct ttccgccatc gcttccagcg acgtaaacag atgctcgaca
    12541 gaaaaatcat tggcgaagtg ctgagcctgc tcaaggctgc gggaatatac ggcggttaac
    12601 ttgtatttac cgctctcatg ggcggcctcg acgaactggc gagtgatcca gttcgtacca
    12661 atcacagcga aacgtatcat aacgcgtgga tactccataa agggattctg tcgtcaatgt
    12721 agcacgcctt tttcatcttc gacgtgcgca gatacgcatt attctttcgc cggacagcca
    12781 tgctcactaa acggcccaat catgaccgat ggcccgagat atttcggctg gcgatggagg
    12841 atttttgcgt caatcaccgc gctgacacga gcgagtttct cccgcagttg agtataaaaa
    12901 ccgtgcttca tattgaggtc tggcgcgtta aacccgatag cgtcgatgcc gtactgtttt
    12961 gccagccaga tggtgcgttg gttatgaaat tcttgcgaga taatggtgat atggttttcc
    13021 ccaaacacct ttttggcacg cactaccgaa tccagcgttg agaatccggc atagtcgcaa
    13081 aagatcactt tcgccggtac gcctttagcg atcaaagcct gctgcatacc tgatgcttca
    13141 tcgtaatttt tacgtccgtt atcgccactc accagtagcc atttcacttt tccggcgtgg
    13201 tacaacgctg ctgcggtatc aatacgtcgg gtaaagtagc gattacccgg cctcgccccc
    13261 agcaataaac ccacgttacg cgccgggacg gcgttgacat cgccccaggt cagctgcttg
    13321 ctggcgttga ccataacgcg atcggcgata aaaatggtcg ctcctgccac aagtaacagt
    13381 aagcaggcaa tctttagagt ccggcgagaa aaacaaatac ggagaagaag gcgggcaaat
    13441 gcgcgcagca tggtcgttat ccttaacgaa aggggggagg tcgattttag gcgctaaaga
    13501 aagaaaagat aacgcctgac atcattttca gattttgtcc tgcccaagcg acaactgcgt
    13561 tagccacagc cgggtgtcga actcaagctg gtggtactgc ggctccatat ggcagcacaa
    13621 ttgatagaac gctttgttat gctctttctc cttcaggtgc gccagctcgt gcaccacgat
    13681 catgcgcaaa aacggttccg gcgcgttgcg aaacacggta gcgacgcgga tctctgcttt
    13741 tgctttcagc ttgctgccct gcacacgaga aacagcggta tgtaacccga gcgcgttttt
    13801 tagcacatgg atcttattgt catacatcac tttattgatc ggcggggcat tacgcagaaa
    13861 ctgatttttc agatcctgag tatattgcca gagagcttta tcggtggcgt agtcgtgtgt
    13921 tcccggatag cgttttgcca gcacatcgcc cagacgctgt tcgttaatca gtgttcgcac
    13981 ctgcgaaagc agctgctcgg gataaccctg aagataagta agattgctca taaccgccca
    14041 cgaaaaagag aaaaagggta tactcacgca cccttttcag gggcaacgtc gaaattttat
    14101 cattcaggag ggccgatgag ccacttagac aacggtttcc gttcactgac actacaacgt
    14161 tttccggcga cggatgacgt taacccgcta caggcgtggg aagcggcaga tgaatatttg
    14221 ctgcaacagt tggacgacac agaaatcctc ggcccggtgt tgatcctgaa tgatgccttt
    14281 ggtgcgttaa gctgcgcgct ggcggaacat aagccgtaca gcattggcga ctcatacatc
    14341 agtgaactgg cgacgcgcga gaatttacgc ctaaacggga ttgatgagtc gagcgtgaag
    14401 tttctcgaca gcaccgccga ctacccgcaa cagccgggtg tggtgctgat caaagtgccg
    14461 aaaacactgg cattgctgga acagcaactg cgtgcgctgc gcaaagtggt cacgtcggat
    14521 acacgtatta ttgccggtgc caaagcccgt gacattcaca cttccacgct ggaactgttc
    14581 gaaaaagtgc tcggcccgac caccaccacg ctggcatgga aaaaagcgcg cctgattaat
    14641 tgcacgttca atgaaccgcc gctggccgat gcgccgcaga ccgttagctg gaagctggaa
    14701 ggtactgact ggactatcca taaccatgcg aatgtcttct cccgcaccgg gcttgatatc
    14761 ggcgcgcgct tctttatgca acatctgcca gagaatctcg aaggggagat tgtcgatctc
    14821 ggttgcggta atggcgttat tggtctgacg ctgcttgata aaaacccgca ggcgaaagtg
    14881 gtgtttgtcg atgaatcgcc gatggcggtt gcttccagcc gtttgaacgt tgaaaccaac
    14941 atgccagagg cgttggatcg cagcgagttt atgatcaaca acgcgctctc cggcgtggag
    15001 cctttccgct ttaatgctgt gctctgcaac cctccgtttc accaacaaca tgcgctgacc
    15061 gataacgtcg cctgggagat gttccactac gcccgccgct gcctgaaaat caacggcgag
    15121 ctgtatatcg ttgccaaccg ccatctggat tacttccata aactgaagaa aattttcggc
    15181 aactgcacca ctattgcgac gaataataaa tttgtggtgc tgaaagcggt gaagctggga
    15241 cgtcgtcggt aagattggga gttacctgcc ggatgcggcg taaacgcctt atccggccta
    15301 cgttcagcac gttaccccca ggccggataa gacgcggcaa gcgtcgcatc cggcaatgtc
    15361 tgctcagttc tggaatcgcg cttccataaa acaaaaaatc cctgtcacca tcctctacat
    15421 tctcttgttt agcgtttttc tacgtttatt cttccgtcac acagataaat tcatccgttg
    15481 ccaatctgtc aacggatgtt atcatattgc caatttgtca acggacgtga tgaagaatgc
    15541 acctgataac tcaaaaagca ttgaaagatg ctgcggaaaa atacccgcaa cataaaacgg
    15601 agttggtggc tctggggaac acgattgcta agggatattt caaaaaacct gagtcattaa
    15661 aagcagtatt cccatctctg gataacttca aatatctgga taagcattat gttttcaatg
    15721 ttgggggcaa tgaattacgt gttgtagcaa tggtcttttt tgaatcgcaa aagtgctaca
    15781 tacgtgaagt tatgacgcat aaagaatacg atttctttac cgctgttcat cgtactaagg
    15841 ggaaaaaatg attgcgattg ccgacatctt gcaagcagga gaaaagctaa ctgctgtggc
    15901 accttttctg gcgggtattc agaacgagga acaatacacc caggcgctgg aactggtaga
    15961 tcatctgctg ctcaacgatc ctgaaaaccc cttgctggat ctggtgtgtg ccaaaataac
    16021 cgcgtgggaa gaatcagcgc ccgaatttgc ggaatttaat gccatggctc aagccatgcc
    16081 tggcggtata gccgtgattc gtacccttat ggatcaatat ggtttaaccc tttccgatct
    16141 gccggaaatt ggcagtaaat ctatggtgtc acgcgttttg agcgggaaga ggaaattaac
    16201 gctggaacac gctaaaaaat tggcaacgcg attcggcatt tctcccgcct tgtttattga
    16261 ttaacgcgac gtgccgaatg cgttgcatcc ggcgcttcaa ataacgattt aaatctccag
    16321 cgccagccgt gttccctggg caattgcccg tcgtgcgtcc agctccatag ccacatcgca
    16381 gccgccaatt aaatgcacag ttttcccgct atcaatcagc ggttgcgcca gcgcgcggtt
    16441 tggctcttgc cctgcgcaga tcaccacatt gtccactgct aatacctgcg tttcgccgtt
    16501 gatcaccaca tgcagcccgt catcgtcaat cttctgataa cttacgcctg ggatcatttt
    16561 cacaccccgc gagagcaggg tggtgcgatg gatccagccg gtggttttgc ctaacccctg
    16621 tcccggtttg ctggctttgc gctggagcat cacaatttgc cgtgggctac gcgggatctg
    16681 cattccctgc gggcttaagc caccagcctg ttgtaggctg ctgtcgatcc cccactcatt
    16741 acagaacctg gcgatattct ggctggtgga ttcgcccggc tgacttaaat acatcgccgt
    16801 atcaaaacca atcccgccac aaccgatgat ggcaaccttg ttgcctaccg gcgctttgtc
    16861 gcgcagtaca tcgagataac tcaatacctt cggatgatcg atcccgtcga taggcggaac
    16921 acgcggcacg atcccactgg cgaggatcgt ttcatcgaac gcctgtaact gatccgccgt
    16981 cacggtgtga ttgagtttca gcgtcacgcc cgtcacttcg atcatccggc ggtagtagcg
    17041 cagcgtttcg taaaactcct ctttgccggg gatctgtttg gcgatattaa actgcccgcc
    17101 gatctcgctg tgcgcatcaa acaatgttac ctgatgccca cgcgccgccg cgttaatggc
    17161 aaacgccagc ccagcaggtc cagcgccgac caccgccaga ttttttttct gcacggcggg
    17221 aaggattggc attttggttt cgtggcaggc gcgaggattc accaggcacg aggtgacttt
    17281 gccaacgaag atctgatcga ggcagccctg gttgcagcca atacaagtgt tgatctcatc
    17341 ggctcgccct gattgcgctt ttgacagcag ttccgcatcc gcgagaaacg gtcgcgccat
    17401 cgatactata tcggcatcgc cgcgcgagag aatatcgtcg gcaacctgcg gatcgttaat
    17461 ccggttggtg gttaccagcg gcagcgagac gtggcctttc agtttgcgcg tgacccagct
    17521 aaatgcgccg cgcggcacgg gcgtggcgat ggtcggaatc cgcgcttcgt gccagcctat
    17581 tccggtgttg ataatggtcg cgcccgccgc ttcaatggcc tgcgccagtt ctaccgtttc
    17641 ggcaaaagtg ccgccgcctt cgactaggtc gagcatcgac aaccggtaaa taataatgaa
    17701 gtcgttgccg acgcgttcgc gcaccgtacg cactacttct acggcaaatc gcatccggtt
    17761 gcggtaatcg ccgccccact ggtcactacg ctgattggtg cgcagcgtca gaaattcgtt
    17821 gatcaaatac ccttcagagc ccatcacttc gacgccatcg tatccggcct cccgcgccag
    17881 ttgcgcgcag cgggcgaaat cgtcgatcag ttgcaggatc tcttcatggg tgagttcgtg
    17941 aggaacgaaa cggttgatgg gggcctgcaa tgcggacggt gcgaccagat gcggttggta
    18001 gctgtagcgc ccggtatgca aaatttgcag ggcgattttg ccgccttcct gatgtacggc
    18061 ttcggtaatg gtgcgatggt gtgggatctg gctggcgtcg ttgagcattg cgccaccttc
    18121 catgccaacg cctgttaaat ctggtgcaat gccgccgctg acaatcagcg ccacgccgtg
    18181 acgggcgcgt tcggcataaa acgcagccag ccgttcagca ccgtccgggt attcctccag
    18241 cccggtgtgc attgaaccca tcaacacgcg gttttttaac gtggtaaaac ctaaatccag
    18301 cggggcgaac agcgacgggt agctcataaa attgtccagt atgtaaaata attgttatgt
    18361 agtcggatga gttctaattt agccgtcgat ggagaaaagg gaaaagggga gtgcggggtt
    18421 tgtgatggga ttcaaaaaga atacgcccgg tggtaacgcc ggatgcgacg cttgccgtgt
    18481 tttatccggc ttgggggatt gcacatgtag ggcggataag gcgttcacgc cgcatccgcc
    18541 agccgttgca aattactgct tacggaaaaa atcgttatac aacatataca aatgagcggc
    18601 actccaggag aaatttggtg cgccttgctg cgcgcctgtc agcgggttgt aattctcctg
    18661 aattgggcca tcggcggtta atcctttggc gtgcctgaag aacgtatccg ccagcttcag
    18721 ggcatcatca cgataaccgt aacgctccat ccctttcaga ccaaaccaga actgatccac
    18781 ccatacgcgc ccgcgccagt agatatcagc gccaaaagcc ggattggtta acgccgccgt
    18841 tcccagcgga acaaatgtgt tgaactcttt aggatcgagc atcaccttca ccaccgcgtc
    18901 ggcattggcc tgcgttgccg caccgttaaa cagcggcgac cagccttccg gccctttacc
    18961 gcgctcaaca atcggtttgc ccgcgcagcc gttcgccagt ggtttatctt caatacgtac
    19021 gtcatagtag aactgggtag tcgggtcgaa catacaggtg ttgatgtagt ccgcgagctg
    19081 ctgtgccaac tggcgatagc gtttggcctc ttctggctta ccgaggatgg ttgccatctc
    19141 cgccagataa tggttatcgc tgtacatata gctggcctga tccaccgact cctgcaatag
    19201 cgagtagccc agcaacgttc cgtcctgact gcggttttcg gcgaatttca ccgtccagtc
    19261 gctacgtttg ccgccattag cgacatattt atccaactgt tctttgtcga taaaaccaaa
    19321 gacggcggca tcatcacgcc ccgattccca cgacgcagcg acctgtgccg gaatttccag
    19381 actgtcgtac tggcctttct ccaccacgcg ggcgtagttg ttcagcccag actgcgtctc
    19441 ttctttatcg ccttttttca ccgtaaacag catctcgccg ctctcggtgt tgtgggcttt
    19501 gtcgcgggtc gcgccatatt ccggcacccc gttgccgtta tgatcgcggt tacgtaacca
    19561 ccagtcgtga taggccacca gtttcgggta catctctgcc aaccaggctt tatcctgggt
    19621 gacgttgtat acttccatca ccgaccaggc ggcaaggctg ggtttggtat tacgttcgtt
    19681 ccagttgccg ccatcgccac cacgctcggg gctaaggttc cacgctatca ggtcggggac
    19741 aaagcccaca tcctgcggac gcacgctgtc gccaggctgg atctgccagg agaagaccgc
    19801 gcggatattc tctttggcga tatccggatt gaaatgcgcc atcgcaaacg cctgcttcca
    19861 ggtatcccac ggccaggtct gattgccgga gaaccagcgt ccggtcaccg acggcgtgac
    19921 ggtgttataa ttcaccgcac cgcccggtga gcgccagtta ccgttgagcg tttcgatcgc
    19981 tttcaccgcg acgcgcgtct gttccggcgt cgcatccgga ttggttaacc ctttcttcag
    20041 gtattcttcc cagcgttgct gcgaggcggt gagataaaac gccggacgcg ccagaatatc
    20101 gcggatctgc atttgctctt tgctaacttc ctgagcggtc agcagatggg aataggtggt
    20161 gtagagcgtg gtcgaaccgt tgatatgcgc cttactggta aagcgattac cgttgatttc
    20221 agtctgcacc ggcagggatt tatgcacctg atattccgat tcgccggagg tcagcagatc
    20281 ccaggtggcg cgcactttgc caaaggtgac tttcaggcca tcacgggtgg cgctgatttt
    20341 gcgctgatag tcagggtatt cgccagcaat ggttttatcg gaaagcggtt tcccttcttt
    20401 cgcttccagt ttttccagca gttcgccatc ccacaccaga tccagcggtt tattgctgat
    20461 gattttagtt tccagtagtg acgtgcgcgg cgtggcgaag cgcagagtca tttcgacctg
    20521 cacatctttt gctgtcagtt tttgcaccag cgcgccggga atactgtatg cctccagcgt
    20581 gaagttgact ttcttgccgt cttgccagac agtcaggcgg tcgaaattgc tggccataaa
    20641 gttgatgtat tcttccgtca gcagcgcaac gcccggaaag ccgcccatgg tgttcgggcc
    20701 gtctggcaac agatgaccat gccaggctcc gagatcgaaa aacggattaa aacgctggtg
    20761 atcgtcgtaa tcgtaatcct tcatgtactg cggcgcacca gtacggttaa tcacgttttt
    20821 ataattgtcg gcgttagcgg catgagcgga aaaacttatc agcagagcgc aggttactgg
    20881 cgttaaaata gttttgattt tcatgagatt acagtcctta ttaccagtag aaatattgca
    20941 tcaggaagat ttggtcgctg ggatcgttag aggcattttt catgtagaag cggctatcaa
    21001 aagccgtggc gaaacgtccg ccgccatatt cgtaccagat accaaactgc atgaaagagg
    21061 tggtttcatc gtcagcgtgg tcagggcgat gtttggcgta ggtgtaagcg gtggagagat
    21121 aaacgccttt taacgcttca tacatggcgc tcaccatcat cccgctttca cttcccggta
    21181 cgtccagccc gtcggcacgt ccggtatggt gccaggcgcg agcggcaaag ttaggggtaa
    21241 acaggcccgt gagatagagt tgtcctgtgc cgtcggtaat ttccagacca ttcatccagg
    21301 tgatgccttc ggcaaggtca tactggatgt aaccgttcat ccattgccgg ataggtgtat
    21361 ttgtcgtcgt agcgatcgta cttgccaaaa tgtagccagg ctttgctttc gtcatgatgt
    21421 ccggcaggtg tggcggtgac gctataacga aagttgccgc ttaagttctg taccttcagc
    21481 gcggtcataa tgtcgcgggt attgggaatg acgtagccca aatcgggtgt gaagtcgccc
    21541 caccattgca ggtcatccag cgaggaatcc tgacgcagac tgagcatgat ttcggtgcca
    21601 tcattggtac gataaccgcc atagaaacgg ttaatcccac cttcgaaacc accccagcta
    21661 tggtcaggca cctaggcatt accgtcgtgg tcggcctgca cggtccagcc ttcgccgtaa
    21721 atcaggccga accaggagcc gtacttcatc tccaggccac cttcaatata ggtgccgtcg
    21781 ctgtatttat gtttattctc accctctaaa tccatatatc caccaagccc taattcgccg
    21841 taaaaacgaa atgaaggaga agatgtcgag gtaattgcgg gtgtttttgt gtcgtcatgt
    21901 tctgccggac gtgcttcttc tcctgccgca gaaaaggcgt agcaaaacgg cagagcaagt
    21961 aatgctctgc atggtgcagt gattaatttc attgtgttaa tccatgagct aaagaaaaag
    22021 tacagccttt tatcgttgta attataaaaa tataaaggca gaaactattt tccctctcct
    22081 catccaggaa gagggaaaat gcctgctcat tttttattag tctgcttaat caacattgct
    22141 ggctggcgtc gcttcaatat ggcgctcttc agccgttaat ccctttatat atttgctgta
    22201 tttccaccac gcaaagccga ggaagataac aataccgccg acgttataaa aaatgatggt
    22261 cagaatattc gcgccagtcg ggaatgtcga agcgacaaac cctacggcaa atatcgcaat
    22321 cagcattgaa acaacgataa taccggtgcg gcgggagccc atacggaaat cgcgtggcag
    22381 gtgatctaat ttggcgcgta aattcaggta agccagcatg atgaataacg gcggaagcat
    22441 ggacgctgcg gcggtcatat taataatggt attcatcaga tcctgcacgg tattggaacc
    22501 gagcatcggg ataatcatca gcgggatgac gatcagaaac tggatccatg ctgcgcgcgc
    22561 cggaacgccg ttttcgttaa gttcgacggt tttcttacca aagatgcctt ccgggatttc
    22621 ggagaagaaa attttcaccg gcgttgcggt ccacatcagc agggaaccga acattgcggt
    22681 aaaggacacc agaccgacaa agcgattcat caacgcttcc ggcagaccaa aatacgccgc
    22741 catgccgtgg aatacctgca ccgatccgcc ggtaaatttc aactctttgc tgctgacgaa
    22801 gacgttaatc agcaccgagg agacggaata cagtacgccg ataaaaatcc cggcgaggat
    22861 gatcacttta acgaacgatt tcgaaccgcc tttgacgtcg ttaacgtaca ccgcgacgga
    22921 ctccgcgcca cctgcggcca taaagatcca ggtggtaacg ccgaggaacg cccagttgaa
    22981 gttcgggatc atcgcgtcaa cggtgatcgg atcggcaggc tgtacgccgc caaccagtgc
    23041 cgtacctgcc agtaaaatgt aggagagcgt taacagcagc atcagcgttg aagtgacgga
    23101 ggtaattggc cccagcattt tcgccccgtt ggtggaaacc caggtggaga aggcgaacag
    23161 caccatactg ataacggtgg tggcaaccgg cgtcataata tattcgtagc cgaggaaggc
    23221 atacgaagca taagcaataa cgcgcggcaa cagcgaggtg aaaaagaaca gatttacgaa
    23281 ccagtaagtg taggcagtaa taaatgccca acgtccgccc agcgaacttt ttacccacgc
    23341 gtagacaccg gcttctgagt ttttatttaa cgaaacaaat tctgcgatga tcagacagaa
    23401 gggaataaaa taaaaaatcg tcgcgaggaa aaacatcggt gccgaggcca gtccaagctc
    23461 aatattatta ttgataacgt tgttaaagct gtaaacggcg gcaaaagtca gcgagagcaa
    23521 gccgaatttg ccgattgtat tacgtttggt atcagacata acacatctcc gaaaatgtgt
    23581 agggtagcgg tggcggcaaa ctccttttgc cgccgtagtt gttatttgtt atggaaataa
    23641 ccatcttcga tggtgacttt gagaaccact tttttgaccg cgttattgca gataaaccga
    23701 tacgcttcat ggatatcgca aataacgatt tgcccttcgt ggacctctac ggtttctccg
    23761 cagcctttta aatattcacg gtcagtttca tcacgataat attccactac ctgtaatgtc
    23821 tctttcggcg catattcaat tttttgctgc ccttgcaggt agtaatgcac ttcaaaatag
    23881 cgtcgatgcc cggtaaatag cgcatcggtc gcggagtcat tttccacacg gtaggtcaat
    23941 gaatcaccga tggagtgggc gacgccaggc tgaatgttgt cgatattttc aatcgcttca
    24001 acgcagcgtt gccacttctt gccagaggcg taaatctggc ggaactgttc taagttatcg
    24061 atgatcctca ttgcttattc tcgctgtgca aattcgtgga aaagaaccct gcgccgaact
    24121 catacgacgc caggctttgc gcggtagctt ctccgccaga aaccggcagc aacgtaaagc
    24181 cgtagctgaa gtcacggaac cagacgcgcc aggagtccag cacctcgctg ccccaggagt
    24241 tggagccgag gccaagtagc tggtggtcga ggttcagggt gatgtcatca ctgcgctgca
    24301 gctcgttaca gtgctgggca gcgtggatgt tttcctgggt atagtgccag gcgctgaagt
    24361 taattgggcg ctgcggaacc accagcagac cgttgccgtg gcggttagtc agtgccgtcc
    24421 agcggacatg ctgacggttg ccgttgttct gcgggaaggg atagttctcg aacatggcat
    24481 cgacggtgct gcgccagata tcgatgatgt tagcctgctg gctgtcggcg tagttttcgc
    24541 ccggtccacg accgtaatac gccacctgat cgtattcgcc gttaatcccc atggtgaaac
    24601 cgatgcacgg aatgatgtgc ggatagtcgc cgtaacgctc gccggaaagc gccacgttaa
    24661 cctggccatc ggcagcgatg cgccagatgt aggtacagcg catcccgaag tcaaacaccg
    24721 gcggtgcaat aactgtgcgg ctgatgatca acacttcacc atcgctctgt tctacggcaa
    24781 agtcgcgcag atgttcctgc atgatctgca aatgattcgg ctgccacaac ccttcgtact
    24841 cctgcttgtg gttgtcgatc atcggcttga agaagttgat ctttggctcg cgggtcagca
    24901 gcgactcgcc atttacctgc caggatgtcg gtttgccact catttttgag aaggtgatcg
    24961 cgaagttgta gccgcgaacg gtgcagctca aacgatcgtc ttccagcgtc agcggacgcg
    25021 cattatttgg tgcgaaaggc actggctgcg cggtgttttc cttcagcggg aactgataag
    25081 tggcgataga atgcccggct tcgctgtagc gggtgcggga atctttggtc accgtaatgt
    25141 tgaggaacgc ttcgcgggcg tccagctgcg gcagcgtgat ctgcaagggg gcttcgctgt
    25201 tcggcgcaac gtcgcgcagt ttaatctgct gcgtcgcgag cgtttcacct tcggcgcgca
    25261 cctctgcgtg cagggtgtag tcatcaagcg tggtaaacca cagtttattt tcgactttca
    25321 actcgccgcg agtcagcgcg tggattttta ccggcgcgat aacctgtttg tactctttca
    25381 ggcccgatcc cggcgtctga tcggaataga tcaaaccatc aagacagaag ttatagttgt
    25441 tgggatagtc gccgtagtcg ccgccgaatt tataccagac attgccgtgg tcgtcctgtg
    25501 cctggatccc gtggtcgcac cactcccaga cataatgacc ctgaatgcaa tcgtgcttat
    25561 agaagacgtt ctggtactcc gtcagcccgc ccggtccgtt ccccatcgca tgagcatatt
    25621 cacagatgat gcgcggcttc ggatgcgggt attcaccaaa ctcattcatc agcggcacgc
    25681 gggtgtacat ggtggaaata atatcgacca cttcagcatc gcgatcttct tcgtaatgaa
    25741 ccagtcgcgt gtcatccagc gctttcgccg catggtacat cgcgcggatg ttacagccat
    25801 agccggattc attgcccagc gaccagatga tgatcgacgg atggtttttc tgtgcgtgga
    25861 tatggcgaac aatgcgctcg acgtagactt tttcccactg cggatcgtcg gtaatacggc
    25921 tgatatcgcc gacattagca aagccgtgcg attcgacgtc ggtttccgcc atcacaaaca
    25981 ggccgtagat atcacacagt tcgtaaaaac gcggatcgtt cgggtagtga gcggtacgca
    26041 cggagttgat attgtgctgc ttcatcaact ggagatcttt ctcgacgcga tccattccaa
    26101 cggcgcggcc tttgcgatga tcgttgtcgt gacggttgac gccgtgcagc atcacataac
    26161 ggttattgat ccagaacaga ccgtcgcgca ctttgatatc acggaagcca acgcgttgtg
    26221 gcaccacttc cagaacgttg ccgttggcgt ctttcagcgt catgaccaga tggtaaagat
    26281 aaggggattc tgctgaccat tgctgcggct gttcgacagt aaaagcaaag ctggcgctgg
    26341 tcagtttttc aattgccaaa tgatcaatgg cgctgctgtg caccacgcgt tcgccatcaa
    26401 acagggtata ttccagcgtc gtgacgacag gggaggcggc gagattttcc agcaccactt
    26461 cgcaggaaag cgtggcatcg caataggctt cgtcaaagtc ggtacgcaca gtgaaatcgt
    26521 taatatgcgt taggtgtttt ccgaccagat aaacatcgcg gaagatcccc gccgaccacc
    26581 acatatcctg gtcttccacg taggtagagt ccgcccactg catcacgcgc acacacaaca
    26641 ggttgtcgcc ggttttaacc atcgcgctga tgtcaaactc tgcggtcagg cgactgccct
    26701 tgctgaaacc cacatactga ccgttaacgt agacttcaaa ataggtttcg acgccgtcaa
    26761 atttaatcag cgtctgttta ccctgccagc cgtcgctgag ggtgaaaata cgttgatagg
    26821 caccggttgg gttatcgctg gggacaaacg gcacatcgat ggggaacgga aaaccttcgt
    26881 cggtatattg cagtttgccg tgaccttcca tttgccacat ggcggggacg gtaatatgcc
    26941 cccagtcagc cattaactca gaggtgaagg cttctggtac ttgcagcgga tggtcaaaaa
    27001 agtggaaatt ccactgactg cttaagggca gaaacaggct gctggtttcg cgggcaaagg
    27061 tacgcgcttg cgcaacagaa tcatatgaaa aaaagtacgc acgcggcgca agtcggtttt
    27121 cgtgggtgag ctgaatgttt tcccagcgat tcatagggcc tcccggtaga gagaaatcgg
    27181 aaccattcat ggtgatggta gtttgtgtaa ttgctggggg tttagtaaaa ttttttacta
    27241 aaaagcagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    27301 ccggcaaaaa tgattgccga acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    27361 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    27421 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg
    27481 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    27541 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    27601 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    27661 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    27721 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    27781 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    27841 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    27901 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    27961 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgg
    28021 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    28081 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    28141 tgactgcgcc tgtctgtagt ttacgggcgc tactggtctt gtactccagc ttttcggcga
    28201 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    28261 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    28321 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    28381 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    28441 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    28501 ctatagttta gtaaaatttt actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg
    28561 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    28621 ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa ccgtgaccag
    28681 cctggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg tcttatgcaa
    28741 tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac
    28801 cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg cgggcgtacg
    28861 cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat attacgcttc
    28921 ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca ccagttcaca
    28981 ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt tattgagcgt
    29041 tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc cgatttcgtt
    29101 atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta ccagatcggg
    29161 atattcccgc gccagttgac ggaaaccgtc cagcaacata tcgccttttt gctcagcctg
    29221 agccggtaag ttctgctcca gcaacacatt gatggtcgcc agcgccgccg cacaggccag
    29281 cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac
    29341 ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg ccgccgaggg cttttgccag
    29401 acacagaatg tccggctgaa cgttctcatg ttcgcaggca aacatcttgc ccgtgcgccc
    29461 catgcccgtt tgtacttcat cgaggatcat cagtgcgccg aactcatcgc acagcttacg
    29521 cacggcggtg agatagcccg gcggcggcag aattacgcca ccttcgccct gaatcggttc
    29581 gaggatcacc gcagccacat catcaccggt ttttttgcac tcattaagag ccgtgcgcat
    29641 ggcttcgata ttgccaaacg gcacatgacg gaaccctggc agcagtggca taaacggttt
    29701 gcggaaggtc gatttcgccg tggccgacag cgcgccaagt gatttaccgt ggaacacgcc
    29761 gctggtggca ataaaagtaa atttgccgcg cggcgactga taagccttcg ccagcttcag
    29821 cgccgcttca acggactcgg tgccgctatt acagaagaag ctgtatttca gtttaccggg
    29881 cgttagcgca gcaagggttt tcgccagcat agcccgtaac ggatcgagta gctcctggct
    29941 gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa accacaactg gattacggtg
    30001 ccccacgttg aaaattccaa aacctcccag gcagtcgata aactcctgtc cctgggtgtc
    30061 gacaagcgta tttaaacctc ccgcttgcca ctctacggct ccgtaatccc cgccggcggt
    30121 aacagatttg cgatactcta aaaaccccgg attgacatgc tctttgaagt attcaatcac
    30181 ctctcggtta agtgctttca tctcctcatg atccagcgtt cgcttctcaa tgagattcag
    30241 ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt aacctgttca aaatatgctc
    30301 cggaggctcg cgtatcacat gataccgatt taagtattgc agggattgcg ccactccggc
    30361 tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc atttgtgcaa tatttaatgc
    30421 tatccgttaa catttgttgt tattttgcac ggtttttgtc gggtatggat ttgatgattg
    30481 ctgcgcaaat gcacgttttc agggcaataa ttgcctgatt tttgggcaag ataattaacg
    30541 ttttcgtata gtaacagagt gaaagtattt taaatcaaag aattagattt gtgctttaat
    30601 cgcaaattgc gatctaaatc aaattaatcg gttaaagata accgcagcgg ggccgacata
    30661 aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat gtcttctcat
    30721 ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat gtccactacc
    30781 gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag cggctttacc
    30841 ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat gccaaaagcg
    30901 gcgtttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagcgg catcgtgaaa
    30961 aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc gatggtgcgc
    31021 gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga agagattgcg
    31081 gcggtggagc cgctgtacaa agcgttgaac tccggacgta ccagtaagcg tattcataaa
    31141 ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct tcgctggcgg
    31201 acgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctttg gtttgttgct
    31261 gccccggtgg tgacgtattt cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt
    31321 gaatggcaga ttgtgcgccc gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc
    31381 ggagagcgta atagtgttga gcacctgaat cgcagcgatg agctggggct gacattacgc
    31441 gcggtagggc agcttggcct gatgtgccgt tggctaatta acgatgtctc aagccaggtg
    31501 tccagtgtca gaaatggcag tgagacgctg gcgaaaggca ccgatgaact gaacgaacat
    31561 acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca aatggcggcg
    31621 tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat caccgccagt
    31681 aatgcggcag tgcagggtgg ggaggcgatg accacggtga tcaagacaat ggacgatatc
    31741 gccgacagta cctagcgcat tggcaccatt acttcgctga ttaacgatat tgcgtttcag
    31801 accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa
    31861 ggttttgcag tggtggcagg ggaagtgcgt catttagcca gccgcagcgc taatgctgcc
    31921 aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatccgg ttcgcagcag
    31981 gtacacgccg ccggacggac gatggaagat attgtggcac aggtgaaaaa cgtcacccag
    32041 ttgatcgccc agattagcca ttcaacgctg gaacaggccg atgggctttc cagcctgacc
    32101 cgtgcagtgg atgagcttaa cctgatcacc cagaaaaatg ccgagctggt ggaagagagt
    32161 gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc ggtgacggtg
    32221 ctgcattaat cggtatgccg gatccggcgg ttggagcaca atgcctgatg cgatgctggc
    32281 gcatcttatc aggcctgcgg ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg
    32341 tcgcatccgg caattgcacc gcgccactgg cggatgcggc gattaatcca gttgcgtaat
    32401 atcaaaagcg gcgcggtcga tcaccgcaat gatctttttt atttgtgcat cactgatatc
    32461 gctctggttg acgcgtaaat ccagcaccgc tttaaaatta tccagcgccc gcttcatttg
    32521 cgggttctgg cgcagcgccg cgccaacgca acgcgctttg atgcgttctt caatcatctc
    32581 cacttgttcg cggttttctt ccagccactg cgcgccttgt tcggtcagcg caatctgctt
    32641 cttaccacct tcctcttcgc ggatggtaat cagcgactgc tcctgcagaa aatccagcgt
    32701 cgggtagatg acgcccgggc ttggggtgta attcccctgg gttagattct caatcgcttt
    32761 aatcaattcg taaccgtggc tgtcatcgcg cgagagaata tccagaatca ccagacgcaa
    32821 ttcaccgtga ccaaagaaac gttgccgacg accgccgccg cgaccgtggc gaccaccgca
    32881 acattgaccg tgttcatgct ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc
    32941 tttgcagcag ccctcatggc gtggctggcc ttcatgttta caacaccctt cgtgatgatg
    33001 gctcatatca gcctcctttt gcttgattag atatatctaa tttatatctg atgtgagcat
    33061 tactgcaagt acaaaaatga tttttaaacg tggaagcacc tcacatctag gccggataag
    33121 acgttcacgc cgcatccggc agcattgcta tggcactgaa tgccttcaca tacttcagac
    33181 atatcagttc tacaaatcgc ttgcatttat catgattaac aatcattatc atttgcgaat
    33241 tttatttaga tatatctgat taccatcacg aaggcgataa caatgaataa caccccccgc
    33301 tacccgcagc gcgttcgcaa tgatctgcgc ttccgtgaac tgactgtgct acgcgttgag
    33361 cgcatcagcg ccggttttca gcgcattgtc cttggcggcg aggcgttgga cggttttaca
    33421 tcgcgcggct ttgacgatca cagcaaactc ttctttcctc aacctgacgc tcactttgtg
    33481 ccgccaacgg taacggaaga gagcatcgtc tggccggaag gaccacgccc accgtcgcgt
    33541 gactatacgc cactgtatga cgaactacgc catgaactgg cgatcgattt ctttattcac
    33601 gacggtgggg tcgccagcgg ctgggcgata aacttacggt ggcaggtccg cgcggttcgc
    33661 tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg cgatgaatcc ggaatgcccg
    33721 cactgcgccg ccgcctggaa acgttgagca aacttgccgt taaaccgcaa gttagtgcgc
    33781 tggttagcgt gcgggataac gcctgtcagg attatctcgc gcaccttgat ggttttaata
    33841 tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg tctggcgcag atgcaaatcc
    33901 ctgccgatga ttacttcatc tggataaccg gcgaaggtaa agtcgttaag aatttaagcc
    33961 gccgctttga agcggaacag tatgacccac agcgggtacg tgcagcggct tactggcacg
    34021 caaagtaact gatatataaa aagaaaaagg ctgacgattt ctcgtcagcc tttgctatat
    34081 ctggtggccc ctgctggact tgaaccagcg accaagcgat tatgagtcgc ctgctctaac
    34141 cactgagcta aggggccatg gtagcggatt ataaagtaac tccgtgccgc aatccagcca
    34201 ttaccgcgcg cctgctgttt ttataaacaa tgtattttca atccgttata cttttcctgg
    34261 tgatgttaga aaggagtaaa tatggttgag gatattttgg ctccagggtt acgggtcgtg
    34321 ttttgcggta tcaaccctgg gctttcatcc gccgggactg gttttccctt tgctcatccg
    34381 gcaaatcgct tctggaaggt gatatatcag gccgggttta ccgaccgtca gttgaagccg
    34441 caggaggcac agcatctgct ggattatcgt tgtggcatca ccaaactggt agaccgtcca
    34501 acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg caggtgggcg taagctgatt
    34561 gaaaaaattg aagattatca gccgcaggcg ttggctattc tgggcaaaca agcatatgaa
    34621 cagggattca gctagcgcgg tgcacagtgg gggaaacaaa cgctcaccat tggttcgacg
    34681 cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg tttcactgga gaaactggtt
    34741 gaagcgtatc gcgagctgga ccaggcgctg gtagtgcgtg ggcgataa
//
LOCUS       DADLTY010000021        34747 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.021, whole genome shotgun sequence.
ACCESSION   DADLTY010000021 DADLTY010000000
VERSION     DADLTY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.021"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(18..93)
                     /locus_tag="JN334_000857"
     tRNA            complement(18..93)
                     /locus_tag="JN334_000857"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="JN334_000858"
     tRNA            complement(152..228)
                     /locus_tag="JN334_000858"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /locus_tag="JN334_000859"
     CDS             complement(331..1716)
                     /locus_tag="JN334_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7437095.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="JN334_000860"
     CDS             complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="JN334_000860"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HAY7437096.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="JN334_000861"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="JN334_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HAY7437097.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5066)
                     /gene="rffT"
                     /locus_tag="JN334_000862"
                     /gene_synonym="wecF"
     CDS             complement(3999..5066)
                     /gene="rffT"
                     /locus_tag="JN334_000862"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HAY7437098.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEFMVVGKDDGLSD
                     SCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPSQFFW
                     HIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGELLYFP
                     TRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVPMGYP
                     PNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQGIGTL
                     CLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKNTIAF
                     FSPNYLQGWQRALAIAAGEVA"
     gene            complement(5063..6313)
                     /gene="wzxE"
                     /locus_tag="JN334_000863"
     CDS             complement(5063..6313)
                     /gene="wzxE"
                     /locus_tag="JN334_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HAY7437099.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSALMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6315..7445)
                     /locus_tag="JN334_000864"
     CDS             complement(6315..7445)
                     /locus_tag="JN334_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-glucose transaminase"
                     /protein_id="HAY7437100.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7450..8124)
                     /gene="rffC"
                     /locus_tag="JN334_000865"
                     /gene_synonym="wecD"
     CDS             complement(7450..8124)
                     /gene="rffC"
                     /locus_tag="JN334_000865"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HAY7437101.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8102..8983)
                     /gene="rfbA"
                     /locus_tag="JN334_000866"
     CDS             complement(8102..8983)
                     /gene="rfbA"
                     /locus_tag="JN334_000866"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY7437102.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9002..10069)
                     /gene="rffG"
                     /locus_tag="JN334_000867"
     CDS             complement(9002..10069)
                     /gene="rffG"
                     /locus_tag="JN334_000867"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY7437103.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10069..11331)
                     /gene="wecC"
                     /locus_tag="JN334_000868"
     CDS             complement(10069..11331)
                     /gene="wecC"
                     /locus_tag="JN334_000868"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HAY7437104.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11328..12458)
                     /gene="wecB"
                     /locus_tag="JN334_000869"
     CDS             complement(11328..12458)
                     /gene="wecB"
                     /locus_tag="JN334_000869"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAY7437105.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12514..13560)
                     /gene="wzzE"
                     /locus_tag="JN334_000870"
     CDS             complement(12514..13560)
                     /gene="wzzE"
                     /locus_tag="JN334_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HAY7437106.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13572..14675)
                     /gene="wecA"
                     /locus_tag="JN334_000871"
     CDS             complement(13572..14675)
                     /gene="wecA"
                     /locus_tag="JN334_000871"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HAY7437107.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14915..16174)
                     /gene="rho"
                     /locus_tag="JN334_000872"
     CDS             complement(14915..16174)
                     /gene="rho"
                     /locus_tag="JN334_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAY7437108.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16259..16360)
                     /gene="rhoL"
                     /locus_tag="JN334_000873"
     CDS             complement(16259..16360)
                     /gene="rhoL"
                     /locus_tag="JN334_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HAY7437109.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16501..16830)
                     /gene="trxA"
                     /locus_tag="JN334_000874"
     CDS             complement(16501..16830)
                     /gene="trxA"
                     /locus_tag="JN334_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HAY7437110.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16961..18226
                     /gene="rhlB"
                     /locus_tag="JN334_000875"
     CDS             16961..18226
                     /gene="rhlB"
                     /locus_tag="JN334_000875"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HAY7437111.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18360..19844
                     /gene="gppA"
                     /locus_tag="JN334_000876"
     CDS             18360..19844
                     /gene="gppA"
                     /locus_tag="JN334_000876"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HAY7437112.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19891..21912)
                     /gene="rep"
                     /locus_tag="JN334_000877"
     CDS             complement(19891..21912)
                     /gene="rep"
                     /locus_tag="JN334_000877"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HAY7437113.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            21999..22280
                     /gene="ppiC"
                     /locus_tag="JN334_000878"
     CDS             21999..22280
                     /gene="ppiC"
                     /locus_tag="JN334_000878"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HAY7437114.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22326..23801)
                     /gene="ilvC"
                     /locus_tag="JN334_000879"
     CDS             complement(22326..23801)
                     /gene="ilvC"
                     /locus_tag="JN334_000879"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAY7437115.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23951..24841
                     /gene="ilvY"
                     /locus_tag="JN334_000880"
     CDS             23951..24841
                     /gene="ilvY"
                     /locus_tag="JN334_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HAY7437116.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24838..26382)
                     /gene="ilvA"
                     /locus_tag="JN334_000881"
     CDS             complement(24838..26382)
                     /gene="ilvA"
                     /locus_tag="JN334_000881"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HAY7437117.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26385..28235)
                     /gene="ilvD"
                     /locus_tag="JN334_000882"
     CDS             complement(26385..28235)
                     /gene="ilvD"
                     /locus_tag="JN334_000882"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAY7437118.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(28300..29229)
                     /gene="ilvE"
                     /locus_tag="JN334_000883"
     CDS             complement(28300..29229)
                     /gene="ilvE"
                     /locus_tag="JN334_000883"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HAY7437119.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29249..29512)
                     /gene="ilvM"
                     /locus_tag="JN334_000884"
     CDS             complement(29249..29512)
                     /gene="ilvM"
                     /locus_tag="JN334_000884"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HAY7437120.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29509..31155)
                     /locus_tag="JN334_000885"
     CDS             complement(29509..31155)
                     /locus_tag="JN334_000885"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HAY7437121.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31158..31208)
                     /locus_tag="JN334_000886"
     CDS             complement(31158..31208)
                     /locus_tag="JN334_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437122.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31294..31392)
                     /gene="ilvL"
                     /locus_tag="JN334_000887"
     CDS             complement(31294..31392)
                     /gene="ilvL"
                     /locus_tag="JN334_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HAY7437123.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31744..33223
                     /locus_tag="JN334_000888"
                     /pseudo
     CDS             31744..33223
                     /locus_tag="JN334_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(33248..33586)
                     /gene="maoP"
                     /locus_tag="JN334_000889"
     CDS             complement(33248..33586)
                     /gene="maoP"
                     /locus_tag="JN334_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HAY7437124.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33705..34544
                     /gene="hdfR"
                     /locus_tag="JN334_000890"
     CDS             33705..34544
                     /gene="hdfR"
                     /locus_tag="JN334_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HAY7437125.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(34640..34715)
                     /locus_tag="JN334_000891"
     tRNA            complement(34640..34715)
                     /locus_tag="JN334_000891"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34680..34682),aa:Trp,seq:cca)
BASE COUNT         8481 a         9500 c         8778 g         7988 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ctgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgct
      301 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttaccgtg
      361 gcgattaagc ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccgac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 cggtacgccg tgacgggaaa cttttgccat tgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgccaatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gagcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgtac cgccgccaga ttcgccaacg ccaccagcgc
     1321 caccgcgatc aatgcgggta tccactgcgc catctccggg gaccaaaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc
     1441 ggtgagatag ccaaaaaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaaa cgaacagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg aagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaatgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattaactc cctgacctcg gcgttatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcatgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagt
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc caaccacaat
     2941 cgccccgagt gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag tacttcccag gtaaagtaat tggctgagtt
     3061 cagcaccata ctcgggcgcc ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggtgaaaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttaa cgccagccag aacatgccaa caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctgt cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggaaaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgataccc atcaggatca cccacgtaag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cacctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagacc acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccgccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcactggcaa atgctgttcc gtcatatcct gccagaacgg attttccctg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat gccctgctgg
     4261 cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggca cgtaaagcgg cgacatgctc attgctgcgg
     4501 tcgccggagt tccccaccag aatggtcatt tttccttcac gttgccgatc gttcgccatc
     4561 gtattgagag aagggtccat ccgcgtcggg aagtacagca gttctccccg cacctttggg
     4621 tgtgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaaaaaaa atcgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgctcgtc
     4981 gcggccagcg cgtcattgaa aaaccgcaaa acggttcggt tatggtgagg gatatccgat
     5041 cccagtacgt gaatcagtac agtcatgccc gcctacgcca gagtaaaaac acgccacaac
     5101 aaagagaaaa atagacgata taagttgcca tatatgcctg cgccgcgccc agtgcaccat
     5161 gcgcagggat tagccagtgg gcaaatacca tcaataaagt gaactggctg acttccgcca
     5221 gaatataaaa ccgcagtgac gctttggcga tcaccagata accaaagaca taagcgccca
     5281 cttttaacac atcacccact aactgccagg caaagagatc gcgcatagcg gtaaatttat
     5341 tcgacaacag caaccagata gcaaaatcac gcagcaacca gacggtaaaa ctcgctgccg
     5401 ccactgccgg taagacgaat ttcagcgatt taaccacttc ccgggtgata tcgcgctttt
     5461 ccgttagccg cgacaacgtg ggcagcaaat atacgctgaa cgatgccgta ataaattgca
     5521 ggtaggcatc ggaaatactg ctcacccctt gccagatccc cacttcatcc cagctatact
     5581 gcgccgccag cagtttacgc atcatgatgt aagcaacagg caaggtcacc gacgtaatca
     5641 acgccatgag cgtaaatttg ctcaactgcc ctgccagacc gttatcccag ctgggtttca
     5701 gatagcttaa cgggatgaca ccacgtttaa tcaacatgat ggcggcagga attaccacca
     5761 gcgcgggaat cagcgccaga cccagcaacg ccccttcata accgcccaaa cggtaactga
     5821 cgtagtacgc gagaacgcca atcaagctgc cgacaatcag cgataacgca ttacctgcgg
     5881 catcgcgaaa gcctttcatc agcgccagta acaggttgcc ccaggcgatc cccatttgca
     5941 ccagcgccac taaacgcacc agcccctgat agtcggtatt accaaacaat ccctggctga
     6001 ttggcgcagc tgccagcaca aaaaccagcg ccatcagcgc agagaaacca agtaccatcg
     6061 ctgatgaagt gccgaccacg cggcgcagct gttgcggatt atcatggtac tgggcaacgt
     6121 atttggttac accgttaaag atgccagccc cggcaagcac gccgagcacg gtaatcaact
     6181 ggcggaaatt tgccgccagc ccaagccccg ccggaccaaa tgacaccgcc agcaacttac
     6241 cgaccagtaa cccggcacca atcttgacca gtgtactggc cgccgtccac aaggacgctt
     6301 ttgccaacga catatcagga gaagtagttc aacaaagtcg caattaccgt acgctgattg
     6361 acgggcgaca ggttgtagaa cagcggcagg cgcagcaggc gctcgctctc tttggtggtg
     6421 tagcgatctt caccgtggaa ctcaccaaag tgttcccccg cagggcaacc gtgcagcgga
     6481 atgtaatgaa acaccgccat gatttccgct tctttcagaa agttaatcaa cgcgctccgg
     6541 tcatcaatat cccgcagttt aatgtagaac atatgcgcgt tctgcacgca gccatcggga
     6601 atcgacggca gctcgatacg cccggctttc gccagaggcg ctaacgtatc gtagtagttt
     6661 tgccacagcg ccagacgttg ctggttgata cgatccgctg cttccagttg cgcccacagg
     6721 tacgcagctt gcagatcgga catcaaatag ctggagccaa tatcgcgcca ggtatattta
     6781 tcgacctgac cacggaagaa ctggctgcgg ttagtgccct tttcacggat gatctcggct
     6841 cgttcgatta acgctttatc gttaatcagc gtcgcgccgc cttcaccacc cgccgtgtag
     6901 tttttggttt catggaagct aaagcagcca atatgaccaa tggttcccag tgcacgccct
     6961 ttgtaagtgg acatcacgcc ctgagcggca tcttccacca caaacaaatt atgctttttc
     7021 gccaacgcca taatggtgtc catttcgcag gccacacccg cgtaatggac cggcacgata
     7081 acgcgcgttt tgtcggtgat cgctgcttca atcaacgttt cgtcgatgtt catggtgtcc
     7141 gggcgaacat ccacaaagac gattttcgcg ccacgcagca caaaggcatt ggcggtggag
     7201 acaaaggtgt agctcggcat gatcacttca tcgccaggct ggatatcgag cagcagcgcc
     7261 gccatctcca gcgaagcggt gcaggacggc gtcagtaaca ctttggcgct gccaaaacgt
     7321 tgctccagcc actgctggca gcgacgggta aaaccgccat cgccacacag tttgccgcta
     7381 cccattgccg actgcatata gtcgagttcg gttcccacca ccggcggtgc gttaaatgga
     7441 atcatgtgat cacctgtata accagtacgc ggtgctttct acattcgcac cactttgtat
     7501 gtatcgttta agcgcggcgg tgttgcccat ttgggtcgcc acccgcaaag ttgttttacc
     7561 gcgagcatac gcccagttta gcgccgtttg catcagctca gcacctgcac cgcgtccagc
     7621 cagcaggcca attcgcgcat ctgtcgcatt gagttcccgt aaagagacat agccgcgaat
     7681 atcgccggac gccgcacgta gaatcagaca ttgatgatca aaggtgccgc gcacggcatt
     7741 ttcaatccac tgtgcataaa agcgaccgct ggcgtcaggc gcataccacg gcgcacgaaa
     7801 acggctttgc gcaaatgcgg cgctagctaa ctgacgtaat gcgggaatat cggtctcttg
     7861 tgccacgaca gcaccgctat cactgacatt gttcacgggt agcgccaaat caacttcacc
     7921 ttctaccagg gagaatccca gctgttgcag ggcatccagt tcccccgtat ttgatgccgc
     7981 aattttggcc tgcacccgtg accatggcgc taaggcgtct gacgtcagga gcggtgcttc
     8041 agacgtaatg cgcacgatgg cgctgttaac accaaagaag gcgttttccc aggttagtgg
     8101 ctcaatactg gcgcggacgg gcacgaagta actccagcag atattggccg tagccagttt
     8161 tcgctaatga actggcagca cgcttcacac cctcgtcatc gagccagccg ttacgccagg
     8221 cgatctcttc caggcaggca atcttaaagc cctggcgttt ttctactgtc tgcacaaaag
     8281 tgctggcctc aatcaggctg tcgtgagtgc cggtatccag ccaggcaaat ccgcgcccga
     8341 gcagttcaac ggtcaggttg cccgcctcga ggtacatctg gttgatggag gtaatctcca
     8401 gttcaccgcg ctccgacggc ttcacctgct ttgcgtactc cacgacttta ctgtcgtaga
     8461 aataaagccc ggtcaccgcc cagtttgact tcggctgttt tggcttttct tcaagcgaga
     8521 tagcgcggaa attgtcgtca aattccacca cgccaaagcg ttccgggtcc atcacctgat
     8581 agccaaaaac cgtcgcccct tccgtgcgcg ccgcaacatg acgcagcttc ggactgaagc
     8641 cctgaccgaa gaagatgtta tcgcccagca ccagacaaga aggttcacca ttaaggaagg
     8701 tttcaccgat gataaaggcc tgcgccagac cgtccgggct gggctgttcg gcatattcca
     8761 gctggatacc gaactcacta ccatcgccca gcaggcgctg gaaataacct ttatcttccg
     8821 gcgtagtgat gatgagaatt tcgcggatac cggccagcat cagcaccgac agcggatagt
     8881 aaatcattgg cttatcgtaa atcggcaaca gttgcttcga tacgccgcgc gtaatcggat
     8941 gcaatcgggt accggaaccg cccgccagga taataccctt catgtttgcc tccgctgaaa
     9001 attagccttt cagacctaaa cgctcaccct gatagctgcc gtcctgcacc tgcttccacc
     9061 agctttcatt agccagatac cactgaaccg ttttacgcat tccactttca aaggtttcct
     9121 gcggcagcca gccaagttca cgggcaattt tcgaagcatc aatggcataa cgcagatcat
     9181 gccccggacg gtcagcgaca aaggtgatca ggtcacgata gtacgccacg ccgtgcggct
     9241 tgttcgaagc cagttcttcc agcagctcgc aaatggtttc cacaacatcg agattcttac
     9301 gctcgttgtg accaccaata ttataggttt caccgacttt cccggtggtc gccacgcaat
     9361 acagcgcgcg ggcgtgatct tccacataca gccagtcacg gatttgctgc ccgttgccat
     9421 ataccggcag cggtttaccc gccagcgcgt tgaggatcat cagcgggatc agtttttccg
     9481 gaaagtggta aggaccgtag ttattcgagc agttggtgat cagcgtcggc agaccgtagg
     9541 tccgcagcca ggcgcgcacc agatggtcgc tgctggcttt tgacgcggaa taagggctgc
     9601 tcggcgcata cggcgtggtt tcggtgaaga aatcatccgt cgagtgcagg tcaccatata
     9661 cttcgtcggt ggagatatga tgaaaacgga acgctgattt tttatcttcc gtcagcgtat
     9721 tccagtaagc ccgcgccgct tcaagcaatg tataagtccc gacaatgttg gtttcaataa
     9781 acgctgccgg gccgtcaata gaacggtcaa catggctttc ggctgccaga tgcatgacac
     9841 agtctggctg atgctcagtg aatacgcgtg ccagttctgc ccgatcgcag atatcaactt
     9901 tctcaaaggc aaagcgctcg ctttgcgcga ccggtgccag cgacatcagg tttccggcgt
     9961 aggtcagctt atcgaccact accaccgcat cgctcgtttc gttgatgata taacgcacca
    10021 gcgccgagcc aataaacccg gcaccacctg ttatcagaat ttttctcatc agcgccagac
    10081 tcctttggca tcgacgacat actgctgatg gacattgtcg ccattgataa ctttgaactg
    10141 actatgatcg accagcatca ccagcacgtc tgccgttgcc agcgcctcgt caagctgcgc
    10201 cagagtacaa agcccggtca gttttttcgg caactggtgg atgttaggct caacaaccag
    10261 cgtttcgccg ctgtgccact gggcgatcag ttcagcgatt tccatcgccg ggctttcgcg
    10321 caggtcatca atattcggtt taaacgccag accaaagcag gcgattttca gttcactggc
    10381 tcgtttatcg gtagcagcaa ggcagtcggc aaccgccgct ttcacctgat cgataaccca
    10441 gaacggtttg tgatcgttaa cttcgcgcgc ggtacggata agccgcgcct gctgggggtt
    10501 ctgtgccacg ataaaccacg gatcaacagc aatgcagtga ccgcccacgc cagggccagg
    10561 ctgaagaata ttaacgcgag ggtgacgatt cgccaggcga atcagttccc agacgttaat
    10621 cccctgatcg gcacaaatca gcgacaattc attagcaaaa gcgatattca catcgcggaa
    10681 gctgttttcg gtgagcttac acatttccgc cgtccgcgag ttagtgacga cacactcacc
    10741 ttcgaggaaa attttgtaca attcgctggc gcgggccgaa caaaccggcg tcataccacc
    10801 aatcacgcga tcgtttttaa tcagctcgac cataacctgg cccggtaata cacgttccgg
    10861 gcagtaagca atgttgacgt ccgcctgctc gcccacctgc tgtgggaaag tcagatccgg
    10921 gcgcatctct gctaaccatt ctgccatctt ctcggttgac cccaccggcg aggtggattc
    10981 aaggatcacc agcgcgcctt ttttcagcac tggcgcaatg gagcgagcag ccgattcaac
    11041 gtaggtcata tctggctcat gatcgccttt aaacggcgta ggtacagcaa tcagccaggc
    11101 atccgcttca actggcgtcg tgctcgctcg taaaaaaccg ccttctacgg cagtttttac
    11161 tacgctcgcc aaatcaggct cgacgatatg gatttcgcca cgattgatgg tatcaaccgc
    11221 gtgttggttg atatcgacac caatcacctg tttttgccgt gaggcaaacg ctgctgctgt
    11281 aggcagcccg atgtaaccca gtccgataac agaaatggtc gcaaaactca tagtgatatc
    11341 cgattatttt ttaacgcttc cagaatgcga gagcatgcct gaccatcacc atacgggtta
    11401 tgggcgcggc tcatagcttg atattcgttt tcgtctttta aaagacgcgt cacttcctcg
    11461 acaattcgct gcttatccgt gcctaccaga cgcaccgtac ccgccgtcac cgcttccgga
    11521 cgctcagtgg tatcgcgcat caccagcacc ggtttcccca gcgaaggcgc ttcttcctga
    11581 atgccgcctg agtcagtcaa aatcagccag gcgtggttca tcagccagac aaacggtaaa
    11641 tactcctggg gatcgatcag aatgacattt ttcacatgcc ccagaatgcg attgaccggc
    11701 tctctgacgt tcgggttgag atgcaccgga tagacaatct ggatgtcctg gtgcgtggtg
    11761 gcgatgtctg ccagcgcgtg gcagatttct tcaaagccac gaccgaaact ctcacgccta
    11821 tgaccggtca ccagaatcat ctttttatcg ggggcgataa acgggtaatt tgccgccagt
    11881 tctgaacgca gcgtgtcgct gctcatcacc tggtcacgca cccataacag tgcatcaatg
    11941 actgtattac cggtaatgaa gattcggcta tccgcaacgt tttcacgcag caagttttgc
    12001 cgggaagttt cggttggaga gaagtgatac atcgccagat gcccggtcaa tgtacggtta
    12061 gcctcttccg gccacggcga atagagagcg cccgtgcgca gaccagcctc aacgtgacca
    12121 acaggaatac gctgataaaa cgccgccagg ctggttgcca gcgtcgtcgt cgtatcgccg
    12181 tgaaccagca cgacgtctgg tttgaactcg gcaagaatag gttttagccc ttccagaatc
    12241 cgacaggtta tctctgtcag gccctgtcct ggctgcatta tgttgagatc gtagtcaggt
    12301 acaatggaaa agagtttcag tacctgatca agcatctccc gatgctgcgc agtgacgcaa
    12361 actttagcct caaaaaaagg atcttttgcc aacgcatgca ccaacggagc catcttgatg
    12421 gcttccgggc gcgtaccaaa tacagtcagt actttcacat cgattctctt cgaataagcg
    12481 gcgagcgcct ttgcgctcac cgcagcagtg ttgctatttc gagcaacggc gggttaatgc
    12541 gacaccagcc ccgatcagcc ccccgacaat gccccacata atcatcagga aggcacgacg
    12601 tgggctatcg cgttttaccg gttcttccgg cgtacgcaaa tagcgatagg tctgaaaacg
    12661 cggatccagg gttggaccaa cattcagggt gtttaacatg gctcgattct gatcatagtc
    12721 gagatcaaag gccggaccga cggcctgtaa attttccagt cgagcctgga gcattggacg
    12781 cccaagcagg aacatttctg aatcaggtaa ttcctcggca ggcacatctg tcgcactgcg
    12841 cgaaatatta tgctgctcag caattttcag cgcctgctca atactgttca tccggcggtc
    12901 gtagatggct ttcgccacct cttcctgacg cttcacctga gctttcatct ggatggtgcg
    12961 tgccgcccat gcgcctttca gctcatcatt cagatggctg gctgcacgct ggctggcaaa
    13021 agcaacatac tgacgtaaca ggttattagc gtcaggcgcg gtttcggcaa taagcttcac
    13081 gctgtcattg accgcgcgag taaagtctcc ggggataaac tggatgttgt taatcatttc
    13141 atccagcaac gccgcatcgg ctttgctgtt gcccaccatc cgctgtttgt aatagtcggt
    13201 ttgcagccag aactctctgc gggtatccca cgaggccagt tgcataacaa actctttata
    13261 ggcttcgtcc atgaccgatg gttgatcggc agaagccatg tttgaacgga catccaggtt
    13321 acgcaaaaat tgctgctgcg agtaatatcc ccccagcata ttcaccgttg gacgatcggt
    13381 aatcgccgtc gagctccact cctgacgagc aaaaaaagta tacgccagcg cgattaacgc
    13441 aaacgccagc cccatgccaa taatccatag cttcccagcc cacaaggtac gaaacaaccc
    13501 acgaatatcc agttcatttt cagcgtcttc ggccggtttc ccaggcattg gttgtgtcat
    13561 cacatcctca tttatttggt taaattgggg ctgccaccac gatttctacg cagtctgcgt
    13621 tttacgcgct taataaagcg agcaactttc caggcacgct taatgcaata tccatagagg
    13681 aagaatgcta gcaaaaagag caccagcatg acccactccg ggacaaaatg agaatattct
    13741 gccagcacgc caatggaagc gagcagtgct gcggcaaggg taatcagcac aaacgcctga
    13801 cgggaagtaa acccggcacg catgatcaaa tggtgaatat gctgacggtc aggagagaat
    13861 gggctcatgc ctttacgcag gcgacggtac ataatcgcca ccatatccat tagcggaatg
    13921 gcgattatcc acaaagcggt aaccgggctg atgggatggg ttttgccctg ggtcgtttcg
    13981 agcaggatcc agataacggt aaaaccaatc agcgtactgc ccgcatcacc cataaagact
    14041 ttgtagcggc gacccaggat accaaggtta agcatgatgt atggcaggat ggcggcgatc
    14101 atcgcaaagc accagattgc gaggctggtt tgcccgtcga accacaaaat cataccgatt
    14161 gctgcaaacg agacgcagga caacccgccc agcaagccat caatgccatc aaccatgttg
    14221 aacgcattaa tggccgccca gacggcaaat agcgtcagga agtaaccaaa cggtccgagc
    14281 accatctccc aggagccaaa gatataaccc aggctactga gataaagttt gccgaacacc
    14341 atcataacaa tgccaacagc ggcctgtatg gtggcacgga tttttacgct gatatcaaaa
    14401 cgatcatcca gcgcgccaat gaaaacaagc acaccggcac aagcgagata gagagatgca
    14461 tgcggaatat agtaatcgac aattccgaac gtgaagcaaa tccctgcgta aaccgaaatc
    14521 cccccaacga gaggtatcaa tccctggtga cgtttgcgga agtttggttt atccactaaa
    14581 ccgacttttt ttgccacctt acgggcaaaa aacagaaaca gtgtcgtgaa taaaaaaata
    14641 ctgatgagat cagtactcac tgtcagtaaa ttcacaatgc atgctctcag agaaaattat
    14701 tagcagaagt ataaccacga agacctttat tcagaaggga aagtccgttc cgaacccacc
    14761 agccctgtgc aaaaatcaac catttgatat gcaaattgca taacatgcca gcaaattcca
    14821 ggaattttcc taacatcgcc agcgcgacat taagattaca gatataaaag caaaacgcca
    14881 cgtaaacacg tggcgttttt ggcataagac aaatttatga gcgtttcatc atttcgaaga
    14941 aatcgtcatt ggtcttggtc atggccagtt tattaatgag gaattccatt gcatcgattt
    15001 cgcccatcgg gtgaatgatt ttgcgcagga tccacatttt ctgcagttct tcctgagtcg
    15061 tgagcagctc ttctttacgg gtaccggaac ggttgtagtc gatagccggg aagacgcgtt
    15121 tttcagcgat cttacgagag aggtgcagtt ccatgttgcc tgtaccttta aactcttcgt
    15181 aaataacttc gtccatttta gaaccggtat cgataagcgc cgtcgcgata atggtcaggc
    15241 tgccgccctc ttccacgtta cgcgccgcac caaagaagcg tttcggacga tgaagggcat
    15301 tggcgtccac accaccggtc aacactttac ctgacgccgg aacaacagtg ttgtaagcgc
    15361 gcgccagacg agtgatggag tcgagcagaa tgataacgtc tttcttgtgc tcaaccaggc
    15421 gtttggcctt ctcgatcacc atttccgcaa cctgaacgtg gcgagatgcg ggttcgtcaa
    15481 aggtagaagc aacaacttca ccttttacca gacgctgcat ctcggttact tcttccggac
    15541 gttcgtcgat cagcagaacc atcagcacac aatccgggtg gttgtaagca atgctctgag
    15601 caatgttctg cagcagcata gttttaccgg ctttcggcgg tgccacaatc agaccacgct
    15661 gaccacgacc gataggtgat gccagatcca gtacgcgagc agttaaatct tcagtagaac
    15721 cgttaccacg ttccatacgc agacgagagt ttgcgtgcag cggggttaag ttctcaaaga
    15781 ggattttgtt gcgggcgttt tcaggtttgt cgaagttaac ttcgttaact ttcagcagcg
    15841 caaaatagcg ttcaccttct ttcggcgggc gaatcttacc agagatggta tcaccagtgc
    15901 ggaggttgaa acggcggatt tggctagggg aaacgtagat gtcatcagga ccggcgaggt
    15961 aggagctgtc tgcggaacgg aggaaaccaa atccatcctg caatatctcc agtacgccat
    16021 caccaaagat atcttcgcca ctctttgcgt gctgcttcag gatggcaaaa ataatgtcct
    16081 gcttacgcat gcgagccagg ttttccagcc ccatattttc gccgagagtg atcagctcag
    16141 aaaccggcgt attctttaat tcggtaagat tcatagtggt gtgagttctt aaacttgggg
    16201 taaatctcga acttaatgtt gtgaatggta tggcaggatc atccatgcct gtttacggtc
    16261 atcgactcat gtcttttcgc tgcctggtca caggagagta cgcagaactg aaacgacaag
    16321 acggattgag tgacgagcca gaaatctgtt cacttcgcat ttaagataaa acgggaagcg
    16381 ttggaaatta caagattcaa acttaataag gtatgtttaa tacgaagtca acactaagtt
    16441 agcatgactc acgccgggcg tccagttttt agcgacgggg cacccgaaca tgaaattccc
    16501 ttacgccagg ttagcgtcga ggaactcttt caactgacct ttagacagtg cgcccacttt
    16561 ggttgccgcc acttcaccgt ttttgaacag cagcagagtc gggataccac ggatgccata
    16621 tttcggcgca gtgccagggt tttgatcgat gttcagtttt gcaacggtca gtttgccctg
    16681 atattcgtca gcgatttcat ccagaatcgg ggcgatcatt ttgcacggac cgcaccactc
    16741 tgcccagaaa tcgacgagga tcgccccgtc cgctttgagt acatccgtgt caaaactgtc
    16801 gtcagtcagg tgaataattt tatcgctcat atataactcc acaggaataa gcctggcgtg
    16861 ttggtttcgt tgttggtgta acattaacca actaaaggtt gactttattt caccggatac
    16921 gctttcgtaa agcaatagta agctgatatt ctaccacact atgagcaaaa cacatttaac
    16981 agaacagaag ttttccgact tcgccctgca tccgaaggtt gtagaagccc ttgaaaaaaa
    17041 agggtttcat aactgtacgc ccattcaggc actggccctt ccgctgacgc tggcgggtcg
    17101 tgacgtagcc gggcaggcgc aaaccggtac cgggaaaacg atggcgtttc ttacgtcaac
    17161 gtttcattat cttctctctc atcctgcgat tgccgatcgc aaggtgaatc aaccgcgtgc
    17221 cttaattatg gcaccgacgc gtgaacttgc cgtgcagatc cacgccgacg cggaaccgct
    17281 ggcggaagct accggcctga agctgggcct ggcttacggt ggtgatggct atgacaaaca
    17341 gctgaaagtg ctggaaagcg gcgttgacat tctgattggc accacgggtc gtttaattga
    17401 ctacgccaag cagaaccaca ttaacctcgg tgccattcag gtagtggtac tggacgaagc
    17461 cgatcgcatg tacgatctag gctttattaa agatatccgc tggctgttcc gccgtatgcc
    17521 gcctgcaaat cagcgcctca acatgctgtt ctccgccacg ctttcgtacc gggtacgtga
    17581 actggcgttc gagcagatga acaatgccga atatattgaa gtggaaccgg aacagaaaac
    17641 gggccaccgt attaaagaag agcttttcta cccttctaac gaagaaaaaa tgcgtttgct
    17701 gcaaacgctg atcgaagaag agtggccaga cagagcgatt attttcgcca ataccaaaca
    17761 ccgttgtgaa gagatctggg gccacctggc ggcagatggt catcgtgtcg gtttgttgac
    17821 aggcgatgtc gcgcagaaaa aacgtctgcg tattcttgat gaatttaccc gtggcgatct
    17881 ggatattctg gttgccaccg acgttgccgc gcgtggtttg catattccgg cagtgacgca
    17941 cgtctttaac tacgatttac ccgatgactg tgaagattac gttcaccgta ttggtcgtac
    18001 aggtcgcgca ggcgcaagcg gtcactctat cagcctggcg tgtgaagagt atgcattgaa
    18061 tttgcctgct attgagacct atattggtca ctcaattccg gtaagcaaat acaatccgga
    18121 cgcattgatg accgatctgc caaaaccgct gcgcctcacg cgcccgcgca caggcaatgg
    18181 tccgcgtcgt actggcgctc cgcgtaatcg tcgtcgttca ggttaaataa aatgtcatac
    18241 tcaaaatcat tcaaactgta tcaagatggc aagagaatga atcccgatga gcttactgta
    18301 gtaagtgatt cgggtgaaag aatgcagcca acacagagac agattgaagg atgaagagta
    18361 tgggttccac ctcgtcgctg tatgcagcca ttgatctcgg ttcgaatagt tttcatatgc
    18421 tggttgtgcg cgaggtggct ggaagcatcc agacgctgac gcgaattaaa cgcaaagtgc
    18481 gtctggctgc tggcctgaat agcgaaaatg acctctctaa tgaagcaatg gagcgcggtt
    18541 ggcaatgtct gcgcctgttt gctgaacgtc tgcaagatat ccctccctcg caaattcgcg
    18601 ttgtcgctac ggcgacatta cgacttgccg tcaatgcggg tgattttatt gccaaagcgc
    18661 aggaaatcct cggttgtccg gtacaggtga tcagcggtga agaggaagcg cgtctgattt
    18721 atcagggcgt tgctcacacc actggtggtg ccgatcagcg cctggtggtg gatataggcg
    18781 gtgccagtac tgaactggta accggcacgg gtgcacaaac cacctcgttg ttcagcctgt
    18841 cgatgggctg cgtcacctgg ctggaacgct attttgccga tcgtaatctg gggcaagaaa
    18901 attttgatgc tgcagaaaaa gcggcacgcg aagtgttacg tccggttgcc gatgaattac
    18961 ggtatcacgg ctggaaagtg tgcgttggtg cttccggcac cgtgcaggcg ttacaggaaa
    19021 tcatgatggc acaggggatg gatgaacgca ttaccctgga aaagttgcag caattgaaac
    19081 agcgagccat tcattgcggt cggctggagg aactggagat tgacgggctg acgctggaac
    19141 gtgcattagt gttcccgagt ggtctggcga tcctgatcgc catttttacc gaactgaata
    19201 ttcagtgtat gaccctggcg ggcggtgcgc tgcgtgaagg cctggtctac ggcatgttgc
    19261 atctggccgt cgagcaggat attcgcagcc gtacactgcg taatattcaa cgccgcttta
    19321 tgatcgatat tgatcaggca cagcgcgtag ccaaagttgc ggctaacttc ttcgatcagg
    19381 tggaaaacga atggcatctt gaagcaataa gccgcgattt gctcatcagc gcctgtcagc
    19441 ttcatgaaat cggcctgagc gttgacttca aacaagctcc gcaacacgct gcttatctgg
    19501 tgcgtaacct ggatcttccc ggttttaccc ccgcacagaa gaaactgctg gcgacgctac
    19561 tgctcaacca gactaacccg gtcgatctct catcgctgca tcagcaaaat gccgtaccac
    19621 cgcgcgtcgc agaacaactc tgccgtttac tccgcctggc tatcattttt gccagccgtc
    19681 gccgtgacga tctcgtgcca gagatgacat tacaggctaa ccatgaactg ttgaccttga
    19741 cgcttccgca aggttggcta acccaacatc cgctgggtaa agagattatt gctcaggaaa
    19801 accagtggca gagctatgtc cactggccgc tggaagtgca ttaatgagta agtgccggat
    19861 gcgatgctga cgcatcttct ccggccttga ttatttccct cgttttgccg ccatcatcgc
    19921 tttcagattc gccagatggc tttgcccttt ctgcatccgt tcttcggcac tgaccacttt
    19981 gcgctcctgt tcccaaatca gatcatcctg cggcagctcc agcaaaaagc ggctcggctc
    20041 cgggcgcacc agttcgccgt actgacggcg ttctttacac agcgtaaagg tcaattcctt
    20101 ctgggcgcgg gtaatgccga cataggccag ccgccgctcc tcatcgatat tatcttcatc
    20161 gatactgctc tggtgcggca aaaacccttc ttccataccg accatgtaga cataaggaaa
    20221 ctccagccct tttgacgcgt ggagagtcat cagttgcacc tgatccagct cttcttcact
    20281 ctcaccacgc tccatcatgt cgcgtaaagt aaagcgcgtc accacctggg tgagcgtcat
    20341 cggctcatcc agttcgctgc cttccagcat ctccgtcatc cagctaaaca gttggttgac
    20401 gttcttcatg cgcatttcgg cagctttcgt gctgggcgat gtttcgtaca gccaggattc
    20461 ataatccatg ccgtggatca gatcgcgcac cgcggcaatc ggctcccgct ccgccagacg
    20521 ctggatttct gccaaccagt gagtgaagcg ggtcaatgct tcataaccac gtccgctaag
    20581 cgtctgactc aggcccatat caaagctggc ggtaaacatg cttttattgc gcgtcatcgc
    20641 ccactcaccc agctttttca gcgtcgccgg gccaatctct cgcttcggcg tgttaacgat
    20701 acgcagaaat gcgctgtcat cgtccgggtt agtcagcacg cgcagataag ccagcaagtc
    20761 cttgatttca ggacgagaga aaaacgacgt accaccagaa attttgtacg ggatgcggtt
    20821 ttgcatcagg aatttttcaa acacccgcga ctgatagtta ccgcgataaa gaatagcgta
    20881 atctttgtac tgcgttttat tgacgaagtg atgcgcaatc agttcaccag tgacgcgctc
    20941 ggcttcatgt tcttcgttat tcgcgcttaa aacttttagc tctgtgccgt aacccagttc
    21001 ggagaacaga cgcttttcaa agacgtgcgg gttattggcg atcaggatgt tcgccgcttt
    21061 caaaatacgt ccggaagagc ggtagttctg ctcaagctta atcaccttca gcgccggaaa
    21121 atcctggctc agcagcacca ggttttgcgg acgtgcgccg cgccaggagt agatcgactg
    21181 gtcatcgtca cccaccacgg taaagcgcgc gcggctgccc accagcagtt tcaccagttc
    21241 atactggctg gtgttggtgt cctgatattc gtccaccagc aggtagcgaa ttttgttctg
    21301 ccagcgctcg cggacttctt cattgcgttg cagaagtagc gtcggcaata aaatcagatc
    21361 atcgaagtcg agcacattac aggctttcag gtgtgcatca tacagcccat aacaatgggc
    21421 aaaaatacgg tcccgctcgc caatcgcact ggctgccgcc tgggacggtg ttttgagatc
    21481 gttcttccag ttagagatgg tcgaaatcaa ctgttgcagg agaactttgt catcttcaat
    21541 cagcccttcg gtcaactctt tgagcaacgc cagctgatcg gtatcgtcaa acagcgaaaa
    21601 gttcgccttc atccccagcg ccgcatactc acgcttgatg atatccagcc ccagcgtgtg
    21661 gaaggttgag atcatcagcc catgcgcctc tttgcgaccc agcgtctgtc cgacacgctc
    21721 tttcatctcg cgcgccgcct tattggtaaa ggtcaccgcc gcgatatgtc gcgcctgata
    21781 accgcacccg cggatcagat gggcgatttt attggtgata acacgagttt taccggaacc
    21841 cgcacccgcc agcaccaggc aggggccggt aacgaattcg acagcttgtt gttggccggg
    21901 gtttagacgc ataggaaaat cgctcaatga aagtcgggag gggaaaataa cagcgtggta
    21961 gtatagccag cctcatcctt accactcaag gcacgatcat ggcaaaaaca gcagcagcac
    22021 tgcatatcct tgtaaaagaa gagaaactgg ctctggatct tctcgtgcag attaagaacg
    22081 gtgccgattt cggcaagctg gcgaagaaac actccatttg cccatcaggc aaacgcggcg
    22141 gtgatttagg tgaattccgc cagggtcaga tggttccggc gttcgataaa gttgtgttct
    22201 cttgtccggt actggagcca accggcccgc tgcacaccca gttcggttac cacatcatta
    22261 aggtgctgta ccgtaactaa tagcaaggcc ttctccggga gaaggccttg aactctgaat
    22321 cgctattaac ccgcaacagc aatacgtttc atatccgtca tatagccgcg cagtttctta
    22381 cctacttgct caatcgcatg gctgcgaatc gcttcattta catcacgcag ctgcgcgtta
    22441 tctaccgcac cttccggaat agctttaccc aaatcgcccg gttgcagttc tgccataaac
    22501 ggtttcagca gcggcacaca agcgtaagag aacagatagt taccgtactc ggcagtatca
    22561 gagataacca cgttcatttc atacagacgc ttacggacga tggtgttggc aatcagcggc
    22621 agttcgtgca gtgattcata gtaagcagat tcttcgatga tgccggaatc aaccatggtt
    22681 tcgaacgcca gttcaacgcc cgctttcacc atcgcgatca tcagtacgcc tttatcgaag
    22741 tactcctgct cgccgatttt gccttcatac tgcggcgcgg tttcgaaggc ggttttgccg
    22801 gtctcttcac gccaggtcag cagtttctta tcatcgttgg cccagtccgc catcataccg
    22861 gaggagaatt cgccggagat gatgtcgtcc atatgtttct ggaacagcgg tgccatgatc
    22921 tctttcagct gttcagacaa cgcgtaagca cgcagtttcg ccgggttaga gagacggtcc
    22981 atcatcagtg tgataccgcc ctgcttcagc gcttcggtga tggtttccca accgaactga
    23041 attagttttt ctgcgtatgc cggatcggtg ccctcttcca ccagcttgtc gaagcacagc
    23101 agcgagccag cctgcaacat accgcacagg atggtttgct cacccatcag gtcagatttc
    23161 acttccgcaa cgaaggacga ttccagcacg cccgcacggt gaccaccggt tgctgccgcc
    23221 catgctttag cgatcgccat gccttcgcct ttcggatcgt tttccgggtg aacggcaatc
    23281 agcgtcggta cgccgaatcc acgtttgtac tcttcacgca cttcggtgcc agggcatttc
    23341 ggcgcaacca tgactacggt gatgtcttta cggatctgct cgcccacttc gacgatgttg
    23401 aaaccgtgcg agtaacccag cgccgcgccg tctttcatca gtggctgtac ggtgcgcact
    23461 acatcagagt gctgcttgtc cggcgtcagg ttaaccacca gatccgcctg tgggatcagt
    23521 tcttcgtaag tacccacttt aaaaccattt tcggtcgctt tacgccagga cgcgcgcttc
    23581 tcggcaatcg cttctttacg cagagcgtag gagatatcga gaccagaatc acgcatgttc
    23641 agaccctggt tcagaccttg tgcgccacag ccgacgatga ctactttttt accctgaagg
    23701 tagctcgcgc catcggcgaa ttcatcgcgc cccataaagc gacatttgcc cagctgtgcc
    23761 agctgctgac gcagattcag tgtattgaag tagttagcca tggtgattcc tcgtgatgtt
    23821 gtgcttctta ttgttcggtt cgcgtttgcg agattgaatt cactatatga caggaaattt
    23881 attgcggaaa ttgatatatt cacaacgtca cattgcaatt tctgcaacgt caacattgag
    23941 ggctgcgcca ttggatttac gcgatctgaa aaccttcctg catctggcgg aaagccgcca
    24001 ttttggccgc agcgcgcggg cgatgcacgt tagcccgtcc acgctctcac ggcagattca
    24061 gcgcctggaa gaagatctcg gccagccgct gtttgtgcgc gataaccgca cggtgacgct
    24121 gactgaagcg ggcgaagaac tgcgcgtttt cgcccagcaa acgctgttgc agtatcagca
    24181 gctgcgccac accatcgacc agcaagggcc gttgctctat ggcgaattac atatcttctg
    24241 ctcggtgacc gccgcttaca gccatctgcc gccgattctg gatcgcttcc gcgcggaaca
    24301 cccgtcggtg gagatcaaac tcactaccgg tgatgcggca gatgcaatgg aaaaagtcgt
    24361 cacaggtgaa gctgatctgg cgattgccgg gaagccagag actctgcccg gcgcggtggc
    24421 gttttcgatg ttggagaatc tggcagtggt gttgattgca cccgcattgc cctgcccggt
    24481 gcgtaatcag gtttctgtcg aaaagccgga ctggtcaacg gtaccgttta tcatggccaa
    24541 tcaggggccg gtgcgccgcc gcattgagtt gtggttccga cgcaataaaa tcagtaaccc
    24601 gatgatttac gccacggttg gcgggcatga agcgatggta tcgatggtgg cgctcggctg
    24661 tggcgtggca ttgttgccgg aagtggtgct ggaaaacagc cccgaaccgg tgcgtaaccg
    24721 cgtgatgatt ttagagcgca gcgatgagaa aacgccgttt gagctgggcg tgtgtgcgca
    24781 gaaaaagcgg ctacatgagc cgctaattga ggcattctgg aagattttgc cgaaccatta
    24841 acccgccaaa aagaacctga acgccgggtt attggtttcg tcgtggcaat cgtagcccag
    24901 ctcattcagc cgggtttcga aatccggttc atggtcgcca agttcgaacg ccgccagtac
    24961 gcgcccgtag tcggtgccat ggctgcgata gtggaacaaa gaaatgttcc agtgcgtacc
    25021 cagcgtgttg aggaagcgca gcagcgcgcc cggtgattcc gggaattcga agctgtagag
    25081 gcgttcctgc aacggatgtg acggacgccc gccgaccata tagcgcacat gcaacttcgc
    25141 catttcgtcg tcggagagat ccaccacgct gtagccgccg tcgttgagca tctgcaaaat
    25201 ttctttgcgc tcttcgaggc cgcggctcag gcgcacgccg acaaagatgc aggcgttttt
    25261 ggcatcggca aaacggtagt tgaactcggt gaccgatcgc ccgccaagca gttggcagaa
    25321 tttgaggaag ctgccttttt cttccggaat ggtcaccgcc aacaacgctt cacgctgttc
    25381 gcccagttcg cagcgttccg agacgtagcg caggccgtgg aagttcacgt tggcaccgga
    25441 aagaatatgc gccagccgtt cgccgcgaat gttgtgctgg gcgatatatt ttttcattcc
    25501 cgccagcgcc agcgcgccag agggttccgc caccgcacgc acatcttcga ataaatcctt
    25561 catcgccgca cagatcgcat cgctatcgac ggtgatgatg tcgtcgagat actcctggca
    25621 taaacggaag gtttcgtcac cgatgcgttt taccgcgaca ccttcggcga acagtcccac
    25681 gcgtggcaga tcaaccggat gacccgcatc cagcgctgct ttcaggcagg cggagtcttc
    25741 cgcttctacg gcgatcactt tgatttgcgg catcagttgt ttgatcagca ccgctacgcc
    25801 cgccgccaga ccgccgccgc cgactggcac aaatacgcgg tcgagatggg cgtcctgctg
    25861 gagcagttcc agcgccagcg tgccttgccc ggcaatcacc atcggatggt cgaacggcgg
    25921 cacccaggtg aacccctgct gctgtgacag ttcgatcgct ttagctttcg cttcgtcaaa
    25981 gttcgcgcca tgaagcagca cttcgccgcc gaatccacgt accgcatcga ctttgatgtc
    26041 ggcggtggcg gttggcataa cgatcagggc cttcacgcct aaccgcgcag aagaaaacgc
    26101 gacgccctgc gcgtggttac ccgcagaagc agtgatcacg ccgtgcgctt tttgttcttc
    26161 cgtcaggccc gccatcatgg cgtatgcgcc gcgcagctta aagctgtgca ctggctggcg
    26221 atcttcgcgc ttcaccagaa tgacgttatc aagacgcgac gacagttttt ccattttttg
    26281 tagcggcgta acctgcgccg cctcgtaaac cggcgcgcgc agcactgctc ttaaatattc
    26341 ggcaccttcc ggagcaccgg acaggggttg cgagtcagcc attattaacc ccccagtttc
    26401 gatttatcgc gcaccgcacc tttgtcggcg ctggttgcca ggctggcgta ggcgcacagg
    26461 gcaaaggaaa cctgacgttc acggtttttc ggcgtccagg ctttgtcacc ccgagcttcc
    26521 tgcgcttcac gacgcgccgc cagttcggca tcgcttacct gtaactgaat gccacggttc
    26581 gggatgtcga tagcgatcag gtcaccatct tcaatcagac caatgctgcc gccgcttgcc
    26641 gcttccggtg agacgtggcc gatggaaaga ccagaggtgc cgccagagaa acgaccgtcg
    26701 gtgatcagcg cacaggcttt accgagaccc attgatttca ggaagctggt tgggtagagc
    26761 atttcctgca tgccagggcc gcctttcggg ccttcatagc ggatcaccac cacatcgccc
    26821 gcgacaactt tgccaccgag gatcgcttct accgcgtcgt cctggctttc gtacactttc
    26881 gccgggccgg tgaatttgag gatgctgtca tcgacgcccg cggttttcac gatgcagccg
    26941 ttttcagcaa aattaccgta gagcaccgcc aggccgccgt ctttgctgta ggcgtgttcc
    27001 agcgagcgga tacagccgtt tgcgcgatcg tcatcgagag aatcccaacg gcaatcctgc
    27061 gagaatgcct gtgtagtacg aatgcccgca gggcctgcgc ggaacatatt ttttaccgcg
    27121 tcatcctggg tcagcataac gtcgtattgt tccagagttt gcggcaacgt caggccaagt
    27181 acgtttttca catcacggtt cagtaacccc gcgcgatcca gttcaccgag aataccgata
    27241 acaccaccag cacggtgaac atcttccata tggtattttt gggtgctcgg cgcaactttg
    27301 cataactgcg ggactttacg cgaaagtttg tcgatatcac tcatggtgaa gtcgatttcc
    27361 gcttcctgcg ccgccgccag caggtgaagt accgtgttgg tagagccgcc catcgcgata
    27421 tccagcgtca tggcattttc aaacgccgct ttgctagcaa tattgcgcgg cagtgcactt
    27481 tcgtcgtttt gctcgtagta acgtttggtc aattcaacaa tgcgtttacc ggcattgagg
    27541 aacaactgct tacggtccgc gtgggttgcc agcagcgagc cgttgcccgg ctgcgacagg
    27601 cccagcgctt cggtcaggca gttcattgag ttagcggtaa acatcccgga gcaggagccg
    27661 caggtcggac acgcggaacg ttcaacctga tcgctctggg agtcagaaac tttcgggtct
    27721 gcgccctgga tcatcgcatc aaccagatcg agtttgatta tctgatcgga cagtttggtt
    27781 ttcccggcct ccatcgggcc gccggaaaca aagatcaccg gaatattcag gcgcagggaa
    27841 gccatcagca tccccggggt gattttgtcg cagttagaga tgcagaccat ggcgtcggcg
    27901 cagtgggcgt tgaccatata ctcaacggaa tcagcgatca gttcgcgaga tggaagtgaa
    27961 taaagcatcc ccccgtggcc catggcaatc ccatcatcca ccgcaatggt gttgaactct
    28021 ttggcaacgc cgccagccgc ttcaatttgt tcggcgacca gtttaccgag atcgcgcaga
    28081 tggacgtgac ccggtacaaa ttgggtgaac gagttcacaa ccgcgataat cggcttgcca
    28141 aaatcggcgt cggtcattcc ggtggcgcgc cacagcgcac gagcacccgc catattacga
    28201 ccatgagtgg tggtggcgga acggtactta ggcatacttt atttactccc agtgtctgtc
    28261 tcgtaaatgg gacggtgcgt gccgtcccat tttttgtatt tattgattaa cttgatctaa
    28321 ccagccccat ttatcttcgg tttcgccagt gaagaggccg aagaaggctt gctgaatgcg
    28381 tttggtaacc gggccacaac ggccttcgcc aatctgaata ccgtctacgc tgcgcactgg
    28441 cgtgatttct gccgccgtac cggacataaa gacttcatcc gccaagtaca gggattcgcg
    28501 cgacagcacc tgctcacgca cttcaattcc cagctctttc gccagtttga tgatggcatc
    28561 acgggtaata cccggcagcg cggaggaggt gaacggtggg gtgaacagca caccatcttt
    28621 cacttcaaac aggttttcgc ctgcgccttc agagatataa ccgttcacat ccagcgcgat
    28681 accttcctga taaccgtggc ggcgcgcttc gctacccacc agcagggaag agagatagtt
    28741 accaccggct tttgccgcag ttgggatggt gtttggtgct gcgcggttcc aggaggaaac
    28801 catcgcatcg atcccctgct ccagcgcttc tgcgcccaga tacgctcccc acgggaaagc
    28861 ggcgataatc acatcggttg agtatcccgc tggcgggttt actcccatgc caacatcacc
    28921 gacgaagatc agcggacgga tataggcgct ggtgagattg tttttgcgga tcacgtcacg
    28981 acaagcttcc atcagctcat caatgctctg tgaaaccggg aagcgataga ttttggcgga
    29041 gtcatgcaga cgctgcatat gctcacgatg gcggaataca accggtcctt tgtgtgagtc
    29101 gtagcaacgg atgccttcaa aaaccgaggt gccatagtgc agcgcgtgcg acatcacatg
    29161 caccttcgcg tcttcccagc gaaccatctc cccattgaac caaatgtaat cagctttctt
    29221 cgtggtcatt tttatattcc ttttgcgctc aggcgcggat ttgttgtgat gtggttgtgc
    29281 tctggcagat ggcaacgtgt gcgacgtcca ccagtttatt taactgacta aacagtaagt
    29341 cgaccgaccg tgggctggca acggtcaatt cgatatttat attttgtgca tcgctggcgg
    29401 cagccatatt cattgagcag acgtggaaac cacgatgacg caccacgcgt aaaacacgtt
    29461 ctaaggtttc cggattgaag cgagccgata cattgacctg atgttgcatc atgataattt
    29521 ctccaacatt tctgaattac tggcacctgg cggcaccagc ggccagacgt tctcaagttc
    29581 gtcgattgag acatgaagca ggtatggccc atcactgttc agcatggtgt cgagtgccgc
    29641 ttcaacctgg tctttacggg tgatgtgttg gccagggatg ccgaaggcgc tggctaacat
    29701 gaggaaatcg gggttatcgg taagggtggt ttcgctgtat cgttcctgaa aaaacagttg
    29761 ctgccattgt cgaaccatcc ctaaccgttg gttatcgagt aagacgattt tcaacggtaa
    29821 ctgcttgcgt tttacggtgc ccagctcttg cacattcatc atgaaagagc cgtcaccgga
    29881 gatacagaca acggtatcgt tcggtcgcgc gacttgtgcg ccaactgccg ccggtaaacc
    29941 aaaacccatg gtacctaagc cgctggaggt gatgaaattt tccgggcgag tgtgggcgat
    30001 gtgctgcgca gcccacatct ggtgctgccc cacatctgtg gtcacgacgc aatccgcagg
    30061 tttacgatcc gacagttgtt ttaacaacaa cggagcgtag atagcgtcac cgggatggtc
    30121 gtaacgccag gaatgttcat cacgcagctg cgcgcagtgt tgctgccagt cattgatatt
    30181 taacggctgc tgtaatgctg gtaacagagc atttaaatca ccttgtaatg ccacatgtgc
    30241 ctgacgcagc ttgttcattt ctgccgggtc gatatccata tggataacac tggcgtgtgg
    30301 tgcgaaggtg ttcagtttgc cggtcacccg gtcatcaaaa cgtgcaccca cggcgatcag
    30361 caggtcacac tcctgcaccg cgaagtttgc cgctttggtt ccgtgcatcc ccagcatgcc
    30421 cagatagtac ggataatctg cttctactgc gcccagccct ttcagcgtac aggtggcagg
    30481 catttttgtg gcagcgagaa attcacgcaa agccggaact gcctgcgcca tccccacgcc
    30541 accgccaacg tacagcatcg gtttttgcgc ttttgccagc atctggcgcg cttgctcgac
    30601 ttcggcatgt gggaaagtca cttcgttttc aacggtggtg aaccacggtt ccagatcgcc
    30661 gctggctaac tggatatctt ttgggatatc gaccagaacc ggaccaggac gacctgagct
    30721 ggcaacgtcg aatgcttcag ccatgatgcg cggcaactct tccagagact gcaccagaaa
    30781 gctgtgcttg gtacaggcta acgacaatcc caggacatcc acttcctgaa atgcgtcggt
    30841 gccgataaac ggtgcggaca cctgaccagt gatggcaacg acagggatgg aatctaacag
    30901 tgcgtccgca agcccggtta tcaggttggt tgcgcccgga ccagacgtgg cgatacatac
    30961 gccagtttta ccggtagcac gggcataacc gatagccgcc attgccgcac cctgctcatg
    31021 tcggcacagc aagtgctcca cgccgccgtc atacaatgca tcgtaaaccg gcataattgc
    31081 gccacccgga taaccgaaaa cggtgttcac accctgtgcc cgcaacgcat gtaccaccca
    31141 ctgtgcgcca ttcatagtta gttccccgtc ctgaatcttg agaaacagaa ttttgtgctg
    31201 ttattcattg tctgctcctc ggttatgttt ttaaggtcaa aaaaaccccc ggacctttcg
    31261 gtgcgggggt cttagttcgt taaggcttga tctctaagcc tttcctcgtc caagtgcagc
    31321 cccgcacggt gggataataa tcaccaccac gctaatcacg accaggctaa tcactcgtag
    31381 aagggctgtc attttgtctt ttcttgcatc ttgttcgaag gaatgcctaa agagttacca
    31441 tagattttgc gaatagcaca agatattttt gggcctattt ttatgatggc tttaataatt
    31501 tcaatagaaa ttgttgtttt ttaaaaagaa aatagtgagt gaaaaaattc aatttcagta
    31561 taaatgttgt tcccttcctc gtagttcatc ctttgtccgc tgcatttttg cgagcatcat
    31621 tccaccgcca gaacaactca acttcacttg cacaaataaa caggaatcag ccactaaaat
    31681 tcacgaatta gcgggctaca gcagcgatat cttatgcaca taatggtgac tcaaggaggg
    31741 tttatgtcac tgtcaattgt tcatacccgc gcagccctgg aagtaaatgc gcccccaatc
    31801 actgttgagg tacatatcag taaaggtcta cccggcttaa cgatggtggg cttaacagaa
    31861 acaacggtga aagaagctcg cgatcgcgtg cgcagcgcca ttatcaatag cggatatgaa
    31921 tatccggcga aaaaaatcac catcaacctt gctccagccg atctgccaaa agaaggggga
    31981 cgatatgatt tacctatcgc cattgcgttg ctggcggcct cagaacagct tacagccaat
    32041 aagttagatg aatatgaatt agtcggagaa ctagcgctta caggcgctct gcgtggcgtt
    32101 cccggtgcaa tctccagtgc aactgaagct attaagtcgg gcagaaaaat tatcgtcgcg
    32161 aaagataacg aagatgaagt ggggctaatt aacggtgaag gatgcctgat agccgatcat
    32221 ctgcaggctg tctgtgcgtt tctggaaggt aagaacactc tcgaacgccc gaaaccaact
    32281 gatgcagtat cccgggcgct acaacatgat ctcagtgatg ttgtcggtca ggagcaagga
    32341 aaacgaggac tggaaattac cgccgctggt gggcacaacc ttttactgat tgggccgccg
    32401 ggaacaggta aaacaatgct cgccagccgt attaatggcc tgttgccaga tttaagcaat
    32461 gaagaggcac tggagagcgc tgcgatatta agtctggtaa atgctgaatc agtacaaaaa
    32521 caatggcggc agcgcccgtt ccgctcacct catcacagtg cgtcgttaac tgcgatggtg
    32581 ggcggtggtg caattccagg gccaggtgaa atttcgttgg cgcataacgg cgtgcttttt
    32641 cttgatgagc tacctgaatt tgaacggcgt acactggatg ccttgcgaga gccgattgaa
    32701 tccgggcaga tccatctttc acgcacacga gcaaaaataa cctatccagc ccgtttccag
    32761 cttgttgcgg cgatgaatcc cagccctacc ggacattatc agggaaacca taaccgctgc
    32821 acgccagaac agacattacg ttatctcact ggagatccca ttaccacccc ccggcatttt
    32881 gagtaaaacg gtagtgccgg gagaaagcag cgccaccgtt aaacaacgcg taatggccgc
    32941 cagagagcgc caatttaagc ggcagaataa actgaatgcc tggctggata gtccggaaat
    33001 acgccaattc tgcaaacttg agagcgaaga tgcgatgtgg ttggaagaaa cgctgatcca
    33061 tctggggtta tcgattcgtg cctggcagcg gttactgaaa gttgcacgaa ccattgctga
    33121 tattgatcag tctgacatta tcacacgtca gcatttgcag gaggcagtta gctatcgtgc
    33181 gattgaccgt ttgctcatcc atctgcaaaa actactgaca taaaaaaagg gcatttcgcc
    33241 ctttttatta atcgtcagaa tcggtgtagt cttcagcacc ttcaacctgc ggtttaccgc
    33301 cggaaagggt gtgaaaacgt tttggacgct taatacgcgt catatacttg gaccacacgc
    33361 gttctgcttc tgtcactggc tcacgttcgc cacggcatac tgctacgaag agtttctctt
    33421 cctcggtaac cggctcgcgt ttgccaagat ccaactcatt gaaggcataa ccatgacgct
    33481 caagcagttg tgcctctttg atggtgaaat caccatgacg agagaatcca cgtggataat
    33541 gtttattgtc gaaatatcga ttagtcgtcg taaagctttc cgccatcctg cacgctccta
    33601 attctttgac cgagctagtt atggcgcgga gtattagtta cgcttgacag agtgtaaaac
    33661 aaaacattta aatcataacg acaaataatt ttgcggagag cactgtggat acggaattgt
    33721 taaaaacttt cctggaagtt agccgaacgc gtcactttgg tcgagcggct gaatcgctct
    33781 atctgaccca gtcagcagtg agctttcgaa tcagacaact ggaaaatcaa ctgggtgtga
    33841 accttttcac ccgccacaga aacaatatcc gtttaaccgc tgccggtgaa aaactactgc
    33901 cttatgcaga aacgctcatg agcacctggc aggccgcccg caaggaggtg gcgcatacct
    33961 cacgacataa cgagttttct atcggtgcca gcgcctcgtt gtgggaatgt atgcttaatc
    34021 agtggttggg acgcttgtat caaaatcagg atgcccatac aggcttacag ttcgaagcgc
    34081 gaattgccca acggcagtct ctggtaaaac agctgcatga acgccagctt gatcttctta
    34141 ttaccactga agcgcccaaa atggacgaat ttagtagtca gttgctgggg tatttcactt
    34201 tagcgcttta taccagtgcc ccttctaaac taaagggaga tcttaactat ctgcgactgg
    34261 agtgggggcc agattttcaa cagcatgagg caggtttgat cggtgctgac gaagttccca
    34321 ttctgacaac cagttctgct gaactggcac agcaacagat tgcgatgctt aatggttgca
    34381 cctggctacc cgtcagctgg gcgcgtaaaa aaggcggcct gcataccgtt gtcgatagca
    34441 caacactttc acggccgctt tatgccatat ggctgcaaaa tagcgataaa aatgcgttga
    34501 ttcgcgatct tttgaaaatt aacgtgctgg atgaagtgta ttaatctgaa tggctggcaa
    34561 ggatgccggt agaaggattt acttcggaga ggattatttc agataaaaaa aatccttagc
    34621 tttcgctaag gatgatttct ggcaggggcg gagagactcg aactcccaac acccggtttt
    34681 ggagaccggt gctctaccaa ttgaactacg cccctaatta gggtggcgga acggacggga
    34741 ctcgaac
//
LOCUS       DADLTY010000022        34647 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.022, whole genome shotgun sequence.
ACCESSION   DADLTY010000022 DADLTY010000000
VERSION     DADLTY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.022"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..1224
                     /locus_tag="JN334_000892"
     CDS             <1..1224
                     /locus_tag="JN334_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708695.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7437126.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="JN334_000893"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="JN334_000893"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY7437127.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="JN334_000894"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="JN334_000894"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HAY7437128.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="JN334_000895"
     CDS             2714..3427
                     /locus_tag="JN334_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HAY7437129.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="JN334_000896"
     CDS             complement(3399..4076)
                     /locus_tag="JN334_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY7437130.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPPAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="JN334_000897"
     CDS             complement(4070..4747)
                     /locus_tag="JN334_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAY7437131.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5118)
                     /locus_tag="JN334_000898"
     CDS             complement(4735..5118)
                     /locus_tag="JN334_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAY7437132.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5134..5442)
                     /locus_tag="JN334_000899"
     CDS             complement(5134..5442)
                     /locus_tag="JN334_000899"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437133.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5443..6021)
                     /locus_tag="JN334_000900"
     CDS             complement(5443..6021)
                     /locus_tag="JN334_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY7437134.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="JN334_000901"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="JN334_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="JN334_000902"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="JN334_000902"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HAY7437135.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="JN334_000903"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="JN334_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAY7437136.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="JN334_000904"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="JN334_000904"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAY7437137.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /locus_tag="JN334_000905"
     CDS             10564..11424
                     /locus_tag="JN334_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HAY7437138.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="JN334_000906"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="JN334_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HAY7437139.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="JN334_000907"
     CDS             12291..13031
                     /locus_tag="JN334_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HAY7437140.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="JN334_000908"
     CDS             13198..14148
                     /locus_tag="JN334_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7437141.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="JN334_000909"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="JN334_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="JN334_000910"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="JN334_000910"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HAY7437142.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="JN334_000911"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="JN334_000911"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HAY7437143.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="JN334_000912"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="JN334_000912"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HAY7437144.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /locus_tag="JN334_000913"
     CDS             complement(19724..20617)
                     /locus_tag="JN334_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HAY7437145.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="JN334_000914"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="JN334_000914"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAY7437146.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /locus_tag="JN334_000915"
     CDS             21915..23147
                     /locus_tag="JN334_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAY7437147.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="JN334_000916"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="JN334_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HAY7437148.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="JN334_000917"
     CDS             23768..23827
                     /locus_tag="JN334_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7437149.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="JN334_000918"
     CDS             complement(23928..24530)
                     /locus_tag="JN334_000918"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAY7437150.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="JN334_000919"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JN334_000919"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="JN334_000920"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="JN334_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAY7437151.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="JN334_000921"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="JN334_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HAY7437152.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="JN334_000922"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="JN334_000922"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAY7437153.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="JN334_000923"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="JN334_000923"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAY7437154.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="JN334_000924"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="JN334_000924"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HAY7437155.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29676..29759)
                     /locus_tag="JN334_000925"
     CDS             complement(29676..29759)
                     /locus_tag="JN334_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437156.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30050..31144
                     /gene="gcvT"
                     /locus_tag="JN334_000926"
     CDS             30050..31144
                     /gene="gcvT"
                     /locus_tag="JN334_000926"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAY7437157.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31168..31557
                     /gene="gcvH"
                     /locus_tag="JN334_000927"
     CDS             31168..31557
                     /gene="gcvH"
                     /locus_tag="JN334_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAY7437158.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31676..34549
                     /gene="gcvP"
                     /locus_tag="JN334_000928"
     CDS             31676..34549
                     /gene="gcvP"
                     /locus_tag="JN334_000928"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HAY7437159.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
BASE COUNT         8245 a         8696 c         9399 g         8307 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg ggggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcagag attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tccgttttcg cattatattt tctaatacca tgattttttg atttagtgtt ttttgacatt
    29761 tttttagctc ttaatattgt cttattcaaa ttgactttct catcacatca tctttgtata
    29821 gaaattggtg tattttttgg ttttttattc tgtcgcgatt tttgcatttt ttaaccataa
    29881 gctaatgtga tgatcaattt taccttatgg ttaacagtct gtttcggtgg taagttcagg
    29941 caaaagagaa cgattgcgtt ggggaccggg agtggctccg atgctgggtt ttgtggtgat
    30001 aatttcacca tgaaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    30061 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    30121 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    30181 cggaatgttt gatgtgtcgc atatgaccat cgtcgatctc cgcggcagcc gcacccggga
    30241 gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    30301 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gatctcatcg tctactactt
    30361 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    30421 gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat
    30481 gattgccgtg caagggccga atgctcaggc aaaagctgcc acactgttta atgatgctca
    30541 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggtgggcg atctgtttat
    30601 tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc
    30661 ggccgatttc tggcgtgcgc tggtggaagc gggcgttaag ccctgcggtc tgggggcgcg
    30721 tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat
    30781 ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccgacag atcgtgactt
    30841 tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct
    30901 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca
    30961 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag
    31021 cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa
    31081 ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc
    31141 gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc
    31201 aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa
    31261 catgctcagg agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg
    31321 gttagcgcgg gcgatgactg cgcggttgcc gaatcggtaa aagcggcgtc agacatttat
    31381 gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc cccggaactg
    31441 gtgaacagcg aaccgtatgc aggcggctgg atctttaaaa tcaaagccag cgatgaaagc
    31501 gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga tgagtaacgg
    31561 ctttattcct cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact
    31621 ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac
    31681 acagacgtta agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga
    31741 cgccgcgcaa cagcaagaaa tgctgaatgc cgttggtgca caatcgttaa acgcgctgac
    31801 cggccagatt gtgccgaaag atattcagct tgcgacacca ccgcaggttg gcgcaccggc
    31861 gaccgaatac gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac
    31921 gtcttacatc ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat
    31981 gctggaaaat ccgggctggt ataccgcgta cactccgtat cagcctgaag tctcccaggg
    32041 ccgccttgaa gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat
    32101 ggcctctgct tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa
    32161 acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg gcttccaatg tgcatccgca
    32221 aacgctggat gtggtccgta ctcgtgctga aacctttggt tttgaagtga ttgtcgatga
    32281 cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac
    32341 taccggtgaa attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt
    32401 ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg
    32461 cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc
    32521 acacgcggca ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat
    32581 cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga
    32641 gcaacatatt cgtcgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc
    32701 aaacatcgcc agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa
    32761 ccgcattcac cgtctgaccg atattctggc ggcgggcctg caacaaaaag gtctgaaact
    32821 gcgccatgcg cactatttcg acaccttgtg tgtggaagtg gccgacaaag cgggcgtact
    32881 gacgcgtgcc gaagcggctg aaatcaacct gcgtagcgat atcctgaacg ccgtggggat
    32941 cacccttgat gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagtg tgctgctggg
    33001 cgataaccac gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc
    33061 tatccagcct gcgatgctgc gcgacgacga aatcctcacc catccggtgt ttaatcgcta
    33121 tcacagcgaa accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct
    33181 gaatcaggcg atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat
    33241 gatcccaatc acttggacgg aatttgccga actgcacccg ttttgcccgc cggaacaggc
    33301 tgaaggttat cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta
    33361 cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc
    33421 gattcgtcat tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc
    33481 ttctgcgcac ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt
    33541 ggcgtgtgat aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc
    33601 gggcgataac ctctcctgta tcatggtgac ttatccttct acccacggcg tgtatgaaga
    33661 aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg
    33721 cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc
    33781 gcacctcaac ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg
    33841 accgatcggt gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat
    33901 cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat
    33961 cctgccaatc agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag
    34021 ccaggtggca atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt
    34081 gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct
    34141 gaaagaagaa accggcatca gcgagctgga cattgccaag cgcctgatcg actacggttt
    34201 ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc
    34261 tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat
    34321 tgaccaggtg aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca
    34381 cattcagagc gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt
    34441 cccggcaggc gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg
    34501 cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa taccagtaat tcactgattc
    34561 gagtattttc taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct
    34621 cagacagtcc cctcgcccct ttgggga
//
LOCUS       DADLTY010000023        33689 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.023, whole genome shotgun sequence.
ACCESSION   DADLTY010000023 DADLTY010000000
VERSION     DADLTY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.023"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..524
                     /locus_tag="JN334_000929"
     CDS             <1..524
                     /locus_tag="JN334_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAY7437160.1"
                     /translation="TAFTMLLMMLQASGDPRMAQVLTWISGSTYNATDAQVWRTGIVM
                     VILLAITPLCRRWLTILPLGGDTARAVGMALTPTRIALLLLAACLTATATMTIGPLSF
                     VGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFADWCGRMVLFPFQIPAGLLSTFIG
                     APYFIYLLRKQSR"
     gene            complement(559..1839)
                     /gene="hemL"
                     /locus_tag="JN334_000930"
     CDS             complement(559..1839)
                     /gene="hemL"
                     /locus_tag="JN334_000930"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAY7437161.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            2064..3485
                     /gene="clcA"
                     /locus_tag="JN334_000931"
     CDS             2064..3485
                     /gene="clcA"
                     /locus_tag="JN334_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAY7437162.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            3509..3574
                     /locus_tag="JN334_000932"
     CDS             3509..3574
                     /locus_tag="JN334_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437163.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            3567..3911
                     /gene="erpA"
                     /locus_tag="JN334_000933"
     CDS             3567..3911
                     /gene="erpA"
                     /locus_tag="JN334_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAY7437164.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(3958..4581)
                     /locus_tag="JN334_000934"
     CDS             complement(3958..4581)
                     /locus_tag="JN334_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HAY7437165.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(4619..5419)
                     /gene="btuF"
                     /locus_tag="JN334_000935"
     CDS             complement(4619..5419)
                     /gene="btuF"
                     /locus_tag="JN334_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HAY7437166.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(5412..6110)
                     /gene="mtnN"
                     /locus_tag="JN334_000936"
     CDS             complement(5412..6110)
                     /gene="mtnN"
                     /locus_tag="JN334_000936"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAY7437167.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            6194..7711
                     /gene="dgt"
                     /locus_tag="JN334_000937"
     CDS             6194..7711
                     /gene="dgt"
                     /locus_tag="JN334_000937"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY7437168.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            7841..9265
                     /gene="degP"
                     /locus_tag="JN334_000938"
     CDS             7841..9265
                     /gene="degP"
                     /locus_tag="JN334_000938"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HAY7437169.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            9420..10576
                     /locus_tag="JN334_000939"
                     /pseudo
     CDS             9420..10576
                     /locus_tag="JN334_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
     gene            complement(10665..11051)
                     /locus_tag="JN334_000940"
     CDS             complement(10665..11051)
                     /locus_tag="JN334_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HAY7437170.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(11365..12189)
                     /gene="dapD"
                     /locus_tag="JN334_000941"
     CDS             complement(11365..12189)
                     /gene="dapD"
                     /locus_tag="JN334_000941"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAY7437171.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(12220..14892)
                     /gene="glnD"
                     /locus_tag="JN334_000942"
     CDS             complement(12220..14892)
                     /gene="glnD"
                     /locus_tag="JN334_000942"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HAY7437172.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(14954..15748)
                     /gene="map"
                     /locus_tag="JN334_000943"
     CDS             complement(14954..15748)
                     /gene="map"
                     /locus_tag="JN334_000943"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAY7437173.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            16116..16841
                     /gene="rpsB"
                     /locus_tag="JN334_000944"
     CDS             16116..16841
                     /gene="rpsB"
                     /locus_tag="JN334_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAY7437174.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            17099..17950
                     /gene="tsf"
                     /locus_tag="JN334_000945"
     CDS             17099..17950
                     /gene="tsf"
                     /locus_tag="JN334_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAY7437175.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            18097..18822
                     /gene="pyrH"
                     /locus_tag="JN334_000946"
     CDS             18097..18822
                     /gene="pyrH"
                     /locus_tag="JN334_000946"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAY7437176.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            19114..19671
                     /gene="frr"
                     /locus_tag="JN334_000947"
     CDS             19114..19671
                     /gene="frr"
                     /locus_tag="JN334_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAY7437177.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            19763..20959
                     /gene="ispC"
                     /locus_tag="JN334_000948"
     CDS             19763..20959
                     /gene="ispC"
                     /locus_tag="JN334_000948"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAY7437178.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            21148..21906
                     /gene="ispU"
                     /locus_tag="JN334_000949"
                     /gene_synonym="uppS"
     CDS             21148..21906
                     /gene="ispU"
                     /locus_tag="JN334_000949"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HAY7437179.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            21919..22776
                     /gene="cdsA"
                     /locus_tag="JN334_000950"
     CDS             21919..22776
                     /gene="cdsA"
                     /locus_tag="JN334_000950"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY7437180.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            22788..24140
                     /gene="rseP"
                     /locus_tag="JN334_000951"
     CDS             22788..24140
                     /gene="rseP"
                     /locus_tag="JN334_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HAY7437181.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            24170..26602
                     /gene="bamA"
                     /locus_tag="JN334_000952"
     CDS             24170..26602
                     /gene="bamA"
                     /locus_tag="JN334_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAY7437182.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            26724..27209
                     /gene="skp"
                     /locus_tag="JN334_000953"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             26724..27209
                     /gene="skp"
                     /locus_tag="JN334_000953"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HAY7437183.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            27213..28238
                     /gene="lpxD"
                     /locus_tag="JN334_000954"
     CDS             27213..28238
                     /gene="lpxD"
                     /locus_tag="JN334_000954"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAY7437184.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            28343..28798
                     /gene="fabZ"
                     /locus_tag="JN334_000955"
     CDS             28343..28798
                     /gene="fabZ"
                     /locus_tag="JN334_000955"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAY7437185.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            28802..29590
                     /gene="lpxA"
                     /locus_tag="JN334_000956"
     CDS             28802..29590
                     /gene="lpxA"
                     /locus_tag="JN334_000956"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAY7437186.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            29590..30738
                     /gene="lpxB"
                     /locus_tag="JN334_000957"
     CDS             29590..30738
                     /gene="lpxB"
                     /locus_tag="JN334_000957"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAY7437187.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            30735..31331
                     /gene="rnhB"
                     /locus_tag="JN334_000958"
     CDS             30735..31331
                     /gene="rnhB"
                     /locus_tag="JN334_000958"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAY7437188.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            31368..>33689
                     /gene="dnaE"
                     /locus_tag="JN334_000959"
     CDS             31368..>33689
                     /gene="dnaE"
                     /locus_tag="JN334_000959"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAY7437189.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWL"
BASE COUNT         8085 a         8191 c         9230 g         8183 t
ORIGIN      
        1 gtaccgcgtt caccatgctt ttgatgatgt tgcaggcaag tggtgacccg cgaatggcgc
       61 aagtgctgac ctggatttcc ggttcgacct acaacgcgac cgatgctcag gtctggcgca
      121 ccggaattgt gatggtgatt ttgctggcga ttactccgct gtgccgccgc tggctgacca
      181 ttttaccgct gggtggtgat accgcgcggg cggtagggat ggcgctgacg ccgacgcgaa
      241 ttgcgctgct gctgttagcg gcttgcctga cggcgaccgc gacgatgact attggaccgt
      301 tgagttttgt tggtttaatg gcaccgcata ttgcgcggat gatgggcttt cgacggacga
      361 tgccacacat cgtaatttcg gcgctggtgg gtggtttact gctggtgttc gctgactggt
      421 gtgggcggat ggtgctgttt ccattccaga tcccggcggg gctgctgtcg acctttatcg
      481 gtgcgccata ttttatctat ttgctgagaa agcagagccg ttaatagaat gcctcgcccg
      541 gtgggcgagg caaaattatc acaacttcgc gaacacccga cgtgcagcat cgatggtgtt
      601 attgatatct tccatgctgt gcgccacaga cataaagcct gcttcaaacg ctgacggtgc
      661 caggtaaaca ccttcatcca gcatcatatg gaagaaacgc ttaaaacgct cgacgtcaca
      721 ggccatcaca tcctgatagc acgtcacgga ctcggcgtcg gtaaagaaga taccgaacat
      781 accaccgacg tggttaacga ccagcggaat tcccgcttct tctgccgctt ccagcagacc
      841 ttctgccaga cgtgttgtca gctcatccag cgtttcgtga atgcccggct gcgcgacttc
      901 attcagacag gcgaaacccg ctgccatcgc aatcgggtta ccggaaagcg tacccgcctg
      961 atagaccgga cctgtcgggg ccagcgcatc cattacatca cgacgaccac cgaatgcgcc
     1021 taccggcatt ccaccgccga tgatttttcc gaggcaggtt aaatctggca ctacggcgaa
     1081 ataatcctgt gcgccagcta gcgctacgcg gaaaccggtc atcacttcat cgatgatcag
     1141 caacgcgcca aattcgtcgc acagcgcacg cagacctggc aggaactctg gcagcggcgg
     1201 aacacagttc atattgcctg ccaccggctc gacgataata caggcaatct cttgcgggta
     1261 ttgctcaaac gcggcgcgta cagaggccag atcgttataa gtacaggtta aggtatgttt
     1321 ggcgaaatct gccggaacgc ccggcgagtt tggctggcct aacgtgagtg cgccagaacc
     1381 ggctttcacc agcaggcagt cagcgtgacc gtggtaacaa ccttcaaatt taatgatttt
     1441 gtcgcgaccg gtaaaaccac gggccaggcg gatggcgctc atcgtcgcct cggtgccgga
     1501 gttcaccatg cgcaccatat ccatggtcgg gaccagttcg gtcaccagtt gcgccatttt
     1561 cacttccatt tcggtcggtg caccaaagct taaaccacgc tcggcggctt caatcacggc
     1621 attgcggatc gccggatggt tatggcccag caccatcggc ccccaggaac cgacataatc
     1681 gatataggct ttgccatcaa catcgtacag ataagcgccg tccgcttttt cgataaacag
     1741 tggagtgccg cccacgccag taaaggcgcg aacaggggag ttcacaccgc cagggatcag
     1801 ctcgcgcgct gcgctgtaaa gattttcaga cttactcatg gagggttcct gattcgtaga
     1861 aaaagtgaat ggctgctatt ccatgttatt cataacaagt taaatactcg tcaaacatca
     1921 ggctgcttgt actggtgcaa tcctgatttc ttagagtata aaagttttgt gcatttgaaa
     1981 cattacgctt tgcaaaggat tttcatggaa cgtgcgagta aaatgccgtc atcttatttg
     2041 tatgaccaat aagtgatcat tggatgaaaa ctgatactcc ctctttagaa acaccgcagg
     2101 ccgcgcgcct gcgacgcaga caactgattc gccaacttct tgagcgcgat aaaaccccgt
     2161 tagccatttt gtttatggcg gcagtcgtcg gcacgcttgt cgggctggct gcggttgctt
     2221 ttgacaaagg tgtcgcctgg ttgcagaacc aacgtatggg ggcgctggta catactgctg
     2281 ataattatcc gctactgtta accgtcgctt ttctctgttc ggcggtgctg gcgatgttcg
     2341 gctacttttt ggtgcgcaaa tacgcgcccg aagcaggggg atcggggatc ccggaaattg
     2401 aaggggcgct ggaagatcaa cgtcctgttc gctggtggcg tgtattgccg gtgaaattct
     2461 ttggcgggct ggggacactc ggcggaggca tggggctagg gcgcgaagga ccaaccgtgc
     2521 agatcggcgg caatatcagt cgcatggtgc ttgatatttt ccgcctgaaa ggtgatgaag
     2581 ctcgccatac gctgctggca accggtgctg ctgcggggct ggctgcggcc tttaacgcgc
     2641 cgctggcggg tattttgttt attatcgaag agatgcgtcc gcagtttcgc tatacgttaa
     2701 tttcgattaa agcggtattt attggcgtca ttatgtcgac cattatgtac cggattttta
     2761 atcatgaagt tgcgttgatt gacgtcggta aactttctga tgcgccgctt aatacgctgt
     2821 ggctttatct gatcctcggt attatttttg gcattttcgg ccctattttt aataaatggg
     2881 tgctggggat gcaggatttg ctgcaccgtg tgcacggcgg caatattacc aaatgggtac
     2941 tgatgggggg ggcgattggc ggtctgtgtg gattgctggg gtttgtggca ccagcaacgt
     3001 cgggcggcgg ttttaacctg attcctatcg ctaccgcggg gaatttcagc atgggaatgc
     3061 tggtgtttat cttcgtcgct cgggtcatta ccaccttact ctgcttctct tccggcgcgc
     3121 cgggcggtat ttttgccccg atgctggcgc tgggtactgt gctgggaacc gctttcggaa
     3181 tggttgccgt tgagctgttt ccgcaatatc acctggaggc ggggacgttt gctattgccg
     3241 gaatgggggc attactggcg gcatctattc gcgcgccgtt aacggggatc attctggttc
     3301 tggagatgac cgataactac cagctcattt tgccaatgat tattaccggt cttggcgcaa
     3361 cactattagc gcaatttacc ggcgggaaac ctctatactc ggcgattctt gcgcgcacgc
     3421 tggcaaaaca ggaagctgag caactggcgc gaagcaaggc cgcatcagcc agcgagaata
     3481 cttgaacgaa ataccagggt attagataat ggcgattatt attgggttag aatttgcccg
     3541 attgccgatg tcgtttggag caaaatatga gtgatgacgt agcactgccg ctggagttta
     3601 ccgacgcagc agccaacaaa gttaaaagcc tgatcgctga cgaagataac ccgaatctga
     3661 aattacgcgt gtatatcacc ggtggcggtt gcagcggctt ccagtatggt ttcacctttg
     3721 atgatcaggt gaacgaaggc gatatgacca tcgaaaaaca gggcgttggc ctggtggttg
     3781 atccgatgag cctgcaatat ctggtcggcg gttccgttga ctacaccgaa ggactggaag
     3841 gttctcgttt catcgtgacc aacccgaacg cgaaaagcac ctgcggttgc ggttcttcct
     3901 ttagcatcta atctgttgtt catgtgccgg ataaactatc cggcacatct tctctcttca
     3961 acgcccattc tcatccagcg caaacgtcgg tagcttaaga tgccaacgaa tagccgccag
     4021 ccgaatcaat agcgtcacga ccatgcccat catactggct gtttccagtg gtacggaaaa
     4081 tgtgtaataa gccgtagcgt ggacaatacc gccgataata caggcagttg cgtagatttc
     4141 tgtacgtaaa atcatgggga tttcgcggac cagtacatca cgaatgatcc cgccgccaac
     4201 gccagtaatg acgcccatac aaaccgcgat taacggaccg gcttccgcat taaaggcttt
     4261 attcacgcta atgccgacaa acaccgccag accaacggcg tccagcaccg gcaacatcca
     4321 ttttggtaag cgtcttggct ggcgcaccag cacgatggtc agcatgctgg tgaccatggc
     4381 aacgaccaga tcggtgggat ctttcaccca aaataccggg ccgtgatcca gcgccatgtc
     4441 gcgaattgtt ccgtcgccta ctgcggtaac cacgcccagt accagaacac caaaagggtc
     4501 catacgcaat tttccggcta acaaaacgcc gtagatggca aataccgctg tgccgactat
     4561 atccagccaa tagacgagca ttgttcaatc cccactgagc acctgaaaag gtcaggcact
     4621 aatctacctg tgaaagcgca ttacagagct gttgtgctgc gaggataata cgtgggcttg
     4681 cacgttcaaa ccagtcactc gtgagaggaa taacgggaat tttgagctgt tcaccccagt
     4741 attgtttgat tttaggaatt tggtccggtc cgcctgtaat gacaatagcc tgtggcgagc
     4801 gtgctaacac ctgttcgcgg ctaacttgcg gccagggaac ccggctgtct ttaaagatgt
     4861 tttctccgcc acaaacttcg agtacctggt tctgaatcga ctcttttcca ctggtaaatg
     4921 gcggattaat gccgaattgc agaaaaacac gttttttagg tttatcagca tattgcgctt
     4981 tcaattgcac gtactgatcc agcagggatt gcgcggcttg ttcggccttg tctggttgcg
     5041 gactccaggg ggccagttga cgtaacgcat tggcaatttg ttcaatgctt gtcgcatcga
     5101 cccacatcac ttttattccc agcgaagcca gctggtcaac ctgccgctcg gcattacctc
     5161 cacgccaggc aatcaccaga tcgggtttca gcgcgacaat gcgttccaga ttcatcccct
     5221 gccaggtgga aacctgctca atcttttgcg cttgtggagg atagtcggaa tagctgctga
     5281 ccccaaccgg cgtgatcccg gcggcaaagg caagttcagt gttggcggga gaaagcgtga
     5341 tgacgcgcgg cgcggcgttg agccacagtg gcgcgagaaa agacagggcg accagcgccc
     5401 tgaacagtga tttagccatg tgccagtttc tgcaccagtg actcaaccat caggctggac
     5461 tgtttagcgg caacagccag gaactcatcg aagctaagat gagactgttg atcggccacg
     5521 tcggagatgg cgcgtacgac aacaaacggg acgttgaaat tgtggcagac atgggcgatt
     5581 gccgtcgctt ccatctctac agcaatggcc tgtgggaagt tgtggcggat tttcgccaga
     5641 ccaacagaac cgttgatgaa agcgtcgccg ctaacaatca ggccacgtac agcgttaaga
     5701 ttcagttcgg caatgcaggc ctcagcggca gcgatcagtt tatcgtcagc tttaaagcct
     5761 gccggacagc ctggtaactg accgtattca taaccaaatg ccgtgacatc cgcgtcgtga
     5821 taacgcgctt cgtccgagac aacgatatcg cccactttca gcgttggtgc caggccaccg
     5881 gcagaaccgg tgttaataat cacatctggc ttgcagtgtt ccaacagcag agtggcaccc
     5941 agcgccgcag cgactttacc gatgcccgat ttcagaagcg caacctcggt tccattcagt
     6001 tggccggtat agatttcgca accgccgaga ctgatagttt gacggttttc gattttgtca
     6061 cgcagcagcg taacttcttc ttccattgca ccaatgatgc cgattttcat agatttactc
     6121 gcgataagcc cgatttgaag gcatagttta ccatgcgctt acggggaagc gtatttctca
     6181 cgcgggagag gacatggcac agattgattt ccgaaaaaaa ataaactggc atcgtcgtta
     6241 ccgttcaccg cagggcgtta aaaccgaaca tgagatcctg cggatcttcg agagcgatcg
     6301 cgggcgtatc atcaactctc cggcaattcg tcgtctgcaa caaaagaccc aggtttttcc
     6361 actggagcgc aatgccgccg tgcgcacgcg tcttacccac tcgatggaag tccagcaggt
     6421 gggacgctac atcgccaaag aaattttaag ccgtctgaaa gagcttaaat tgctggaagc
     6481 atacggcctg gatgaactga ccggcccctt tgaaagcatt gttgagatgt catgcctgat
     6541 gcacgatatc ggcaatccgc cgtttggtca ttttggcgaa gcagcgataa atgactggtt
     6601 tcgccaacgt ttgcacccgg aagatgccga aagccagcct ctgactgacg atcgctgcag
     6661 cgtggcggca ctacgtttac gggacgggga agaaccgctt aacgagctgc ggcgcaagat
     6721 tcgtcaggac ttatgtcatt ttgaggggaa tgcacaaggc attcgtctgg tgcatacatt
     6781 gatgcggatg aatctcactt gggcacaggt tggcggtatt ttaaaatata cccgtccggc
     6841 gtggtggcgt ggcgaaacgc ctgagacaca tcactattta atgaaaaagc cgggttatta
     6901 tctttctgaa gaagcctata ttgcccgctt gcgtaaagaa cttaatttgg cgctttacag
     6961 tcgttttcca ttaacgtgga ttatggaagc tgccgacgac atctcctatt gtgtggcaga
     7021 ccttgaagat gcggtagaga aaagaatatt taccgttgag cagctttatc atcatttgca
     7081 cgaagcgtgg ggccagcatg agaaaggttc gctcttttcg ctggtggttg aaaatgcctg
     7141 ggaaaaatca cgctcaaata gtttaagccg cagtacggaa gatcagtttt ttatgtattt
     7201 acgggtaaac accctaaata aactggtacc ctacgcggca caacgattta ttgataatct
     7261 gcctgcgatt ttcgccggaa cgtttaatca tgcattattg gaagatgcca gcgaatgcag
     7321 cgatcttctt aagctatata aaaatgtcgc tgtaaaacat gtgtttagcc atccggatgt
     7381 cgagcagctt gaattgcagg gctatcgggt cattagcgga ttattagaga tttatcgtcc
     7441 tttattaagc ctgtcgttat cagactttac tgaactggta gaaaaagaac gggtgaaacg
     7501 tttccctatt gagtcgcgct tattccacaa actctcgacg cgccatcggc tggcctatgt
     7561 cgaggctgtc agtaaattac cgtcagattc tcctgagttt ccgctatggg aatattatta
     7621 ccgttgccgc ctgctgcagg attatatcag cggtatgacc gacctctatg cgtgggatga
     7681 ataccgacgt ctgatggccg tagaacaata accaggcttt tgtaaagacg aacaataaat
     7741 ttttaccttt tgcagaaact ttagttcgga acttcgggct ataaaacgaa tctgaagaac
     7801 acagcaattt tgcgttatct gttaatcgag actgaaatac atgaaaaaaa acacattagc
     7861 actgagtgca ctggctctga gtttaggttt ggcgttatct ccgctctctg caacggcggc
     7921 tgagacttct tcagcaacga cagcccagca gatgccaagc cttgcaccga tgctcgaaaa
     7981 ggtgatgcct tcagtggtca gcattaacgt agaaggtagc acaaccgtta atacgccgcg
     8041 tatgccgcgt aatttccagc agttcttcgg tgatgattct ccgttctgcc aggaaggttc
     8101 tccgttccag agctctccgt tctgccaggg gggccagggc ggtaatggcg gcggccagca
     8161 acagaaattc atggcgctgg gttccggcgt catcattgat gccgataaag gctatgtcgt
     8221 caccaacaac cacgttgttg ataacgcgac ggtgattaaa gtccagctga gcgatggtcg
     8281 taagttcgac gcgaagatgg ttggcaaaga tccgcgctct gatatcgcgc tgatccaaat
     8341 ccagaacccg aaaaaactga ccgcaattaa gatggcggat tctgatgcac tgcgcgtggg
     8401 tgattacacc gtagcgattg gtaacccgtt tggtctgggc gagacggtaa cttccgggat
     8461 tgtctctgcg ctggggcgta gcggcctgaa tgctgaaaac tacgaaaact tcatccagac
     8521 cgatgcagcg atcaaccgtg gtaactccgg tggtgcgctg gttaacctga acggcgaact
     8581 gatcggtatc aacaccgcga tcctcgcacc ggacggcggc aacatcggta tcggttttgc
     8641 tatcccgagt aacatggtga aaaacctgac ctcgcagatg gtggaatacg gccaggtgaa
     8701 acgcggtgag ctgggtatta tggggactga gctgaattcc gaactggcga aagcgatgaa
     8761 agttgacgcc cagcgcggtg ctttcgtaag ccaggttctg cctaattcct ccgctgcaaa
     8821 agcgggcatt aaagcgggtg atgtgatcac ctcactgaac ggtaagccga tcagcagctt
     8881 tgccgcactg cgtgctcagg tgggtactat gccggtgggc agcaaactga ccctgggctt
     8941 actgcgcgac ggtaagcagg ttaacgtgaa cctggaactg cagcagagca gccagaatca
     9001 ggttgattcc agctccatct tcaacggcat tgaaggcgct gagatgagca acaaaggcaa
     9061 agatcagggc gtggtagtga acaacgtgaa gacgggcact ccggctgcgc agatcggcct
     9121 gaagaaaggt gatgtgatta ttggtgcgaa ccagcaggca gtgaaaaaca tcgctgaact
     9181 gcgtaaagtt ctcgacagca aaccgtctgt gctggcactg aacattcagc gcggcgacag
     9241 caccatctac ctgttaatgc agtaatctcc ctcaacccct tcctgaatac gggaaggggt
     9301 tctccttaca atctgtgaac ttcgccacaa ctccatacat cttcatcatc ctttgggcat
     9361 ttgcacaatg ccgtacgtta cgtacttcct tatgctaagc cgtgcataac ggaggactta
     9421 tggctggctg gcatcttgat accaaaatgg cgcaggatat cgtggcacgt accatgcgca
     9481 tcatcgatac caatatcaac gtaatggatg cccgcgggcg aattatcggc agcggcgatc
     9541 gtgagcgtat tggtgaattg cacgaaggtg cattgctggt actttcacag ggacgagtcg
     9601 tcgatatcga tgacgcggta gcacgtcatc tgcacggtgt gcggcagggg attaatctac
     9661 cgttacggct ggaaggtgaa attgtcggcg taattggcct gacaggtgaa ccagagaatc
     9721 tgcgtaaata tggcgaactg gtctgcatga cggctgaaat gatgctggaa cagtcgcggt
     9781 tgatgcactt gttggcgcag gatagccgtt tgcgggaaga actggtgatg aacctgattc
     9841 aggcagagga gaatactccc gcacttctga atgggcgcaa cggctgggga tcgatctcaa
     9901 tcaaccgcga gtggtggcta ttgttgaggt cgacagcggt cagcttggcg tggacagcgc
     9961 aatggcggag ttacaacaac tgcaaaacgc gctgactacg cccgagcgta ataatctggt
    10021 ggcgattgtc tcgctaaccg aaatggtggt gttgaaaccg gcgttgaact cttttgggcg
    10081 ctgggatgca gaagatcatc gtaagcgagt tgaacaactg attacccgca tgaaagagta
    10141 cggccagctg cgttttcgcg tttcactggg caactatttt accggtcctg gcagtattgc
    10201 ccgatcctat cgtacggcga aaacgacgat ggtggtgggt aaacagcgga tgccagaaag
    10261 tcgctgctat ttttatcagg atctgatgtt acctgtgtta ctcgacagtt tgcgtggcga
    10321 ctggcaggcc aacgaactgg cgcgaccgct ggcgcggctg aaagcgatgg acaataacgg
    10381 cttgctgcga cgaacgctgg cggcgtggtt tcgccacaat gtgcaaccgc tggcaacgtc
    10441 aaaggcgttg tttattcatc gtaataccct ggagtatcgg cttaatcgta tatcggaact
    10501 gaccgggctt gatttgggca attttgatga caggttgctg ctgtatgtgg cgttacaact
    10561 ggatgaagag cggtagatta tgcgttaagg ttgaggcggc gctggctcat tcccctcagc
    10621 ccgaagggga gaggggaatg cccgagttaa ccgtcaattc gggattattt acgcgttagt
    10681 ttttccagat cggcttcaat ctcgctgatc ttattggtta cgactgactc caggtgacgt
    10741 aagtcgtcga ggatcttacg cttaagatca acttcgctgc ggtcacgctg acagatttga
    10801 tcaagctcat caatgatata ccgtagattc gggctgattt cctggacttc tttataaccc
    10861 tgacccacac catcagcgac gaccgtttta cgctgacgcg gatatttaaa cttaacgctc
    10921 ttggcgaaaa actcgccttt gtctttctgg aaatagattt tcagaatatc gttgttggct
    10981 tcctgccgga ggctgtaacg atcaatttct tcaggattgg taatacccag acttttcaga
    11041 ttgtcgtaca tagcgttacc tcaaaatgag tcagtaaatt gtgcttattt tagcatttgg
    11101 cttggcccgc ccggctcgac tgtgatcgca gaaagccttt ttcttccaga cttctatcat
    11161 ggcgtaaaaa tcaaaaaatt acctgcttta ttgtggcgat attccatggg atggattagc
    11221 acatttatct tcgttttgcg aattacatga cagtccgaaa caagatgaaa aagtatttaa
    11281 aaaaggggga ggaagaatga gtaggacgaa ttattctgag ccagaaagca tacgggcagc
    11341 atcatgctgc ccgtatatgt actcttaatc gatggtacgc agcagttcgt taatgccgac
    11401 tttgccgcga gttttcgcgt caactttctt aacgataacc gcacagtaga ggctgtattt
    11461 gccatctttt gacggcagat tacctgaaac aaccacagac cccgccggaa cgcgaccgta
    11521 gtggatttcg ccggtttcac ggtcgtaaat acgggtgctc tgaccaatgt atacgcccat
    11581 agagatgacg gaaccttctt cgacaatcac cccttcaacc acttcagagc gtgcgccaat
    11641 gaagcagtta tcttcaataa tggtcgggtt agcctgcagc ggctccagca cgccgccgat
    11701 gcccacgcca ccggaaaggt ggacgttttt accaatctgc gcacaagaac cgacggtcgc
    11761 ccaggtatca accatggtgc cttcatcaac atatgcgccg atgttaacgt aagacggcat
    11821 cagcacggtg ttacgggcaa taaacgcacc ctgacgtacc gccgctggtg gcacaacgcg
    11881 gaagccttct ttctggaaac gtgcttcgtc gtagtcggcg aatttcatcg gcactttgtc
    11941 gaagtagcgg ctttctgccc cttcgatcac ctgattatca ttaatacgga aagagagcag
    12001 caccgctttt ttcaaccact gatgcgtcac ccactgaccg tcaatttttt ccgctacacg
    12061 cagtgcgccg gaatccagca gggcgatcac ctgatttacc gcttcgcggg taacggtgtc
    12121 tgcattggct ggcgtgatct cggcacggcg ttcaaaagcg gtttcaataa tgttctgtaa
    12181 ctgctgcatt gttaaactct tttcatatca gtaaacacat caccctttat cgtttggatt
    12241 gagggcctct gtcaaccgct gatgcacttc ctgctgcaac tcgttattaa gcgcacgccg
    12301 gtcagcggtg gcaattatga ataaatcttc tactcgctcg ccaatggttg taattcgggc
    12361 accatgaagc gaaattccca gatcggcaaa aattttcccg actcgcgcca gcagtccagg
    12421 ttggtcgagg gcgatcagtt cgaggaacga tttgcggtcg gtatgggtcg gcaaaaacgt
    12481 tacttcggtt tcaacagtaa aatggcgtaa tttggcgggt tggcgacggg gctgcggtgg
    12541 ctgccagcta ctttgcgtca gcacctgctc cagaccaaac cgaataacct catggcgatc
    12601 tgcggacagc gggctgccat cgggttccag cacgataaag gtatccatcg ccataccgtc
    12661 gcgagtggtg aaaatttgtg cgtcgtgaac acttaaattg cggcggtcta attcggcaca
    12721 gacggcggca aacagatagg ggcggtccgg gctccagata aaaatctcgg tgcctccacg
    12781 cgtagcctgc gggctaagca ataccagcgg tttgcttaaa tcatgctgta ataaatggcg
    12841 ggcatgccag gccagttgat ttgggctatg gcggacaaaa tagttagcac gacagcgtga
    12901 ccaaatttgg tgcagcgcct cttcgtcgat gttatccatg cgcagtagtg ccagtgcctg
    12961 gagttggtga tggcgaaccc gttcgcgcat atccggcgtg ttttgcattc cgcgtcgtag
    13021 ctgcttttcg gtggcaaaat agagttcgcg caacagactt tgcttccagc tattccacag
    13081 tgtttcgttg gtggcgcaaa tgtcagccac tgtcaggcat accagatagc gcagacgatt
    13141 ttccgtttga acttcttcgg caaactgctt gatgacttca gggtcctgaa tatcgcggcg
    13201 ttgtgctgtc accgacatca gcaagtgctg gcgaaccagc caggcaacca gctgtgtttc
    13261 gcgtgagttc agcccgtgga gttctgcaaa atgcactaca tcctgagcac cgagaatgga
    13321 gtggtcgccg ccgcgtcctt tggcgatatc gtgaaacagc gcggcgatga aaatcagctc
    13381 agttgacggc aggcgcggcc agacgtccac acacaacgga tggcgctggc gcgtttcttc
    13441 actggcaaaa ctctccagtt tcagcatcac gcggatagta tgttcatcca ccgtgtaggc
    13501 gtggaacaga tcaaactgca tctgcccgac gatatgcgac cattgcggca tatacgcgcc
    13561 gagcacgcta tggcgatgca ttggcaatag tccgcgccgc accgctccgg ggtgacgcag
    13621 aatgctcaaa aacagttttc gtgcttgcgg aatattacac agcggttgtt gcagatggcg
    13681 acgggcatgg cgtaactggc gcagcgtggt ggagtaaatg ccggtgatcg cactgttgcg
    13741 caccatggtg tagaacatac gcaagatggc ttccggctgg cgcataaata gtgtttcatc
    13801 acgcaggtcg attagcgtac cgcgtagctg aaactcatcg tcgagtggac gtggtttttc
    13861 gtcggcggga agggcgagga tggcttcatc gaacagttgc agcagcatct ggttgagttc
    13921 actgacgcgg cgtgtaacgc ggaagtaatc cttcatcatc cgctcgaccg gttcgttgcc
    13981 ttcaccactg taattcagac gctgagcgac gctaagttgg cgatcgaata acagacgatt
    14041 atcgtaacgg ctgacgacca gatgcagggc aaagcgaata cgccacagta tatgcagaca
    14101 ttcgtttaat tccgcccgct ccgctgaggt taagaagcca aacccgacca tttcatccag
    14161 cgatgttgcg ccaaaatgac ggcgggccac ccattgcaga gtgtgaatat cgcgcaagcc
    14221 gccagggcta cttttgatgt ctggttcaag gttgtagctg gtgccatggt aacgctgatg
    14281 gcgctggttc tgttcttcaa ctttcgctgc gtagaacttg tcggaaggcc agaatccttc
    14341 gctaaaaata tgtttttgca gttcgaggaa gagcgcaaca tcgccaatta ataagcggga
    14401 ttcgattaaa ttggtggcga cggttaaatc cgataaccct tccaacatgc actcttcaag
    14461 cgtgcgcaca ctatgaccga cttccagctt tacatcccag agcagcgtta acagctcgcc
    14521 cactttttgc gcctgatcgt ccgggagctt tttacggctt aaaatcagta aatcgatgtc
    14581 agaaagtgga tgcagctcgc cacgaccgta gccaccgacg gcaaccaatg ccaggtcggc
    14641 aatctggctg aatcccgctt caatccataa tcgttgcagg agctggtcta tgaactcggt
    14701 acgtgcctca atcaactgtt ctgcagagat cccattgtca aaggcatcac ccagccaacg
    14761 ctggaaagta tcgatatggg cttttatccc acagacggtt aattcatcac ggggccaggc
    14821 gcatggattt tgcggttgac cgggcagggt ggagagagcg gtgtttgcgt actgttctgg
    14881 aagggtattc attgtgcgcc acccataaga ttaaattatc gcattaaaaa agccggcatt
    14941 cgccggcttc atcttattcg tcgtgcgaga ttatcgccgg gatggtgtca tccttgcgta
    15001 gcgtcaaaat ttcgcagccg ttatcagtca ccacaatagt atgctcatat tgtgcagaca
    15061 agctgcgatc tttggttttt accgtccagc catctttcat ggtgcggatc tcttttttac
    15121 ccgcgttgac cattggctca atggtgaacg tcatccccgg tttcagcacg acgttggttt
    15181 cacgggagtc atagtgcagc acctgcggtt cttcatggaa gccgcgacca ataccgtgtc
    15241 cgcaatattc acgaacgacg gagaagcctt ctgcttcgac aaatttctga atcgccgcac
    15301 cgatttcgcg cagattaatg cctggtttta ccatgcgtag cgccaggtac aggctttctt
    15361 gcgtgatgcg gcacaggcgt tcgcccatga tggtcggctt accgacgata aacattttcg
    15421 aggtatcgcc gtggaaacca tctttgatta cggtgacatc aatgttaacg atatcgccat
    15481 ctttcagcag cttagcatcg tccgggatac cgtggcacac cacttcatta atagagatgc
    15541 aaacggattt cggatagccg tgatagccga ggcaggcaga aaccgcgtgt tgttcattaa
    15601 caatgtaatc attacagatg cgatccagct cgccggtgct gacgcccggt ttaacatacg
    15661 gttcgatcat ctccagcact tcggcagcca gtcggccagc gacgcgcatt ttttcgatat
    15721 cttctggggt cttgattgag atagccatta attctgtcca tcagcgtcgg tgataccgac
    15781 aatatatatg taagtgccgt caatggtatc acacccgggc aaattgagaa tcattctgaa
    15841 tttcgccaaa cgtgccactg aaggttttct ataatagaaa attcgacgtc tgatgctgta
    15901 cacagcgcca acaattattg gtgtccacga cgtatttgtg gtataaagcg cgccggactt
    15961 ccgatccatt tcgtatacac agactggacg gaagcgacaa tctcactttg tgtaacaaca
    16021 cacacgtatc ggcacatatt ccggggtgcc ctttggggtc ggtaatatgg gatacgtgga
    16081 ggcataaccc caacttttat atagaggttt taatcatggc aactgtttcc atgcgcgaca
    16141 tgctcaaggc tggtgttcac ttcggtcacc agacccgtta ctggaacccg aaaatgaagc
    16201 cgttcatctt cggtgcgcgt aacaaagttc acatcatcaa ccttgagaaa actgtaccga
    16261 tgttcaacga agctctggct gaactgaaca agattgtttc tcgcaaaggt aaaatccttt
    16321 tcgttggtac taaacgcgct gcaagcgaag cggtgaaaga cgctgctctg agctgcgacc
    16381 agttcttcgt gaaccatcgc tggctgggcg gtatgctgac taactggaaa accgttcgtc
    16441 agtccatcaa acgtctgaaa gacctggaaa ctcagtctca ggacggtact ttcgacaagc
    16501 tgaccaagaa agaagcgctg atgcgcactc gtgagctgga gaaactggaa aacagcctgg
    16561 gcggtatcaa agacatgggc ggtctgccgg acgctctgtt tgtaatcgat gctgaccacg
    16621 aacacattgc tatcaaagaa gcaaacaact tgggtattcc ggtatttgct atcgttgata
    16681 ccaactctga tccggacggt gttgacttcg ttatcccggg taacgacgac gcaatccgtg
    16741 ctgtgaccct gtacctgggc gctgttgctg caaccgtacg tgaaggccgt tctcaggatc
    16801 tggcttccca ggcggaagaa agcttcgtag aagctgagta ataaggcttg ataactcccc
    16861 caaaatagtt cgagttgcag aaaggcggca agctcgagaa ttcccgggag cttacatcag
    16921 taagtgaccg ggatgagcga gcgaagacaa cgcatctgcg gcgcgaaata tgaaggggga
    16981 gagcccttat agaccaggta gtacacgttt ggttaggggg cctgcatatg gccccctttt
    17041 tcacttttat atctgtgcgg tttaatgccg ggcagatcac atctccgagg attttagaat
    17101 ggctgaaatt accgcatccc tggtaaaaga gctgcgtgag cgtactggcg caggcatgat
    17161 ggattgcaaa aaagcactga ctgaagctaa cggcgacatc gagctggcaa tcgaaaacat
    17221 gcgtaagtcc ggtgctatta aagcagcgaa aaaagcaggc aacgttgctg ctgacggcgt
    17281 gatcaaaacc aaaatcgacg gcaactacgg catcattctg gaagttaact gccagactga
    17341 cttcgttgca aaagacgctg gtttccaggc gttcgcagac aaagttctgg acgcagctgt
    17401 tgctggcaaa atcactgacg ttgaagttct gaaagcacag ttcgaagaag aacgtgttgc
    17461 gctggtagcg aaaattggtg aaaacatcaa cattcgccgc gttgctgcgc tggaaggcga
    17521 cgttctgggt tcttatcagc acggtgcgcg tatcggcgtt ctggttgctg ctaaaggcgc
    17581 tgacgaagag ctggttaaac acatcgctat gcacgttgct gcaagcaagc cagaattcat
    17641 caaaccagaa gacgtatccg ctgaagtggt agaaaaagaa taccaggtac agctggatat
    17701 cgcgatgcag tctggtaagc cgaaagaaat cgcagagaaa atggttgaag gccgcatgaa
    17761 gaaattcacc ggcgaagttt ctctgaccgg tcagccgttc gttatggaac caagcaaaac
    17821 tgttggtcag ctgctgaaag agcataacgc tgaagtgact ggcttcatcc gtttcgaagt
    17881 gggtgaaggc atcgagaaag ttgagactga ctttgcagca gaagttgctg cgatgtccaa
    17941 gcagtcttaa ttatcaaaaa ggagccgcct gagggcggct tctttttgtg cccatcttgt
    18001 aaattcagct aacccttgtg gggctgcgct gaaaagcgac gtacaatgtc gctagtatta
    18061 attcatttca atcgttgaca gtctcaggaa agaaacatgg ctaccaatgc aaaacccgtc
    18121 tataaacgca ttctgcttaa gttgagtggc gaagctctgc agggcactga aggcttcggt
    18181 attgatgcaa gcatactgga tcgtatggct caggaaatca aagaactggt tgaactgggt
    18241 attcaggttg gtgtggtgat tggtgggggt aacctgttcc gtggcgctgg tctggcgaaa
    18301 gcgggtatga accgcgttgt gggcgaccac atggggatgc tggcgaccgt aatgaacggc
    18361 ttggcaatgc gtgatgcact gcaccgcgcc tatgtgaacg ctcgtctgat gtccgctatt
    18421 ccattgaatg gcgtgtgcga cagctacagc tgggcagaag ctatcagcct gttgcgcaac
    18481 aaccgtgtgg tgatcctctc cgccggtaca ggtaacccgt tctttaccac cgactcagca
    18541 gcttgcctgc gtggtatcga aattgaagcc gatgtggtgc tgaaagcaac caaagttgac
    18601 ggcgtgttta ccgctgatcc ggcgaaagat ccaaccgcaa ccatgtacga gcaactgact
    18661 tacagcgaag tgctggaaaa agagctgaaa gtcatggacc tggcggcctt cacgctggct
    18721 cgtgaccata aattaccgat tcgtgttttc aatatgaaca aaccgggtgc gctgcgccgt
    18781 gtggtaatgg gtgaaaaaga agggacttta atcacggaat aattcccgtg atggataaat
    18841 aagggtaaga ttccgcgtaa gtatcgcggg ggcgtaagtc tggttataag gcgttattgt
    18901 tgcaggcagt ttggtcacgg ccagcgcgca gcaaccggag cgtacaaaag tacgtgagga
    18961 tggcgagcac tgctcggggc caaaatggca aataaaatag cctaataatc cagacgatta
    19021 cccgtaatat gtttaatcag ggctatactt agcacacttc cactgtgtgt gactgtctgg
    19081 tctgactgag acaagttttc aaggattcgt aacgtgatta gcgatatcag aaaagatgct
    19141 gaagtacgca tggacaaatg cgtagaagcg ttcaaaaccc aaatcagcaa aatacgcacg
    19201 ggtcgtgctt ctcccagcct gctggatggc attgtcgtgg aatattacgg cacgccgacg
    19261 ccgctgcgtc agctggcaag cgtaacggta gaagattccc gtactctgaa aatcaacgtg
    19321 tttgatcgtt caatgtctcc ggccgttgaa aaagcgatta tggcctccga tctcggcctg
    19381 aacccgaact ctgcgggtag cgacatccgt gttccgctgc cgccgctgac ggaagaacgt
    19441 cgtaaagatc tgaccaaaat cgttcgtggt gaagcagaac aagcgcgtgt tgcagtacgt
    19501 aatgtgcgtc gtgacgcgaa cgacaaagtg aaagcactgt tgaaagataa agagatcagc
    19561 gaagacgacg atcgccgttc tcaggacgat gtacagaaac tgactgatgc tgcaatcaag
    19621 aaaattgaag cggcgctggc agacaaagaa gcagaactga tgcagttctg atttcttgaa
    19681 cgacaaaaac gccgctcagt agatccttgc ggatcggctg gcggcgtttt gctttttatt
    19741 ctgtctcaac tctggatgtt tcatgaagca actcaccatt ctgggctcga ccggctcgat
    19801 tggttgcagc acgctggacg tggtgcgcca taatcccgaa cacttccgcg tagttgcgct
    19861 ggtggcaggc aaaaatgtca ctcgcatggt agaacagtgc ctggaattct ctccccgcta
    19921 tgccgtaatg gacgatgaag cgagtgcgaa acttcttaaa acgatgctac agcaacaggg
    19981 gagccgcacc gaagtcttaa gtgggcaaca agccgcttgc gatatggcag cgcttgagga
    20041 ggttgatcag gtgatggcag ccattgttgg tgctgctggg ctgttaccta cgcttgctgc
    20101 gatccgcgcg ggtaaaacca ttttgctggc caataaagaa tcactggtta cctgcggacg
    20161 tctgtttatg gacgccgtaa agcagagcaa agcgcaattg ttaccggtcg atagcgaaca
    20221 taacgctatt tttcagagtt taccgcaacc tatccagcat aatctgggat acgctgacct
    20281 tgagcaaaat ggcgtggtgt ccattttact taccgggtct ggtggccctt tccgtgagac
    20341 gccattgcgc gatttggcaa caatgacgcc cgatcaagct tgccgtcatc cgaactggtc
    20401 gatgggacgt aaaatttctg tcgattcggc taccatgatg aacaaaggtc tggaatacat
    20461 tgaagcgcgt tggctgttta acgccagcgc cagccagatg gaagtgctga ttcacccgca
    20521 gtcagtgatt cactcaatgg tgcgctatca ggacggcagt gttctggcgc agctggggga
    20581 accggatatg cgtacgccaa ttgcccacac catggcatgg ccgaatcgcg tgaactctgg
    20641 cgtgaagccg ctcgattttt gcaaactaag tgcgttgaca tttgccgcac cggattatga
    20701 gcgttatcca tgcctgaaac tggcgatgga ggcgttcgaa caaggccagg cagcgacgac
    20761 agcattgaat gccgcaaacg aaatcaccgt tgctgctttt cttgcgcaac aaatccgctt
    20821 tacggatatc gctgcgttga atttatccgt actggaaaaa atggatatgc gcgaaccaca
    20881 atgtgtggac gatgtgttat ctgttgatgc gaacgcgagt gaagtcgcca gaaaagaggt
    20941 gatgcgtctc gcaagctgag gataatccgg ctacagagag tcgcgctatt tgttagcgta
    21001 gggcttcagt gatatagtct gcgccatctg atcgtaagta gttggcttta taaggtcagg
    21061 tatgccgtgg ttttacacgg cttttttttg tataggcttc agtattcctg agtaccgtaa
    21121 accctgtcag ggaataaaaa acgcgtgatg ttgtctgcta ctcaaccact tagcgaaaaa
    21181 ttgccagcgc atggctgccg tcatgttgcg atcattatgg acggcaatgg tcgctgggca
    21241 aaaaagcaag ggaagattcg tgcctttggg cataaagccg gggcaaaatc cgtccgccgg
    21301 gctgtctctt ttgcggccaa caacggtatt gaggcgttaa cgctgtatgc ctttagtagt
    21361 gaaaactgga accgaccagc gcaggaagtc agtgcgttaa tggaactgtt tgtgtgggcg
    21421 ctcgatagcg aagtaaaaag tctgcaccga cataacgtgc gtctgcgtat tattggcgat
    21481 accagtcgtt ttaactcgcg tttgcaagaa cgtattcgta aatctgaagc gctaacagcc
    21541 ggaaataccg gtctgacgct gaatattgcg gcgaactacg gtggacgttg ggatatagtc
    21601 cagggagtca ggcaactggc tgaaaaggtg cagcaaggaa acctgcaacc agatcagata
    21661 gatgaagaga tgttaaacca gcatgtctgt atgcatgaac tggcccctgt agatttagta
    21721 attaggactg ggggagagca tcgcattagt aactttttgc tttggcaaat tgcctatgcc
    21781 gaactttact ttacagatgt tctctggccc gatttcgatg aacaagactt tgaaggggcg
    21841 ttaaatgcct ttgctaatcg agagcgtcgt ttcggcggca ccgagcccgg tgatgaaaca
    21901 gcctgatggg ggtcgctttt gctgaagtat cgcctgatat ctgcttttgt gttaataccc
    21961 gtcgtcatcg cggcgttgtt tctgttgccg ccggtggggt tcgccattgt aacgctggtg
    22021 gtctgcatgc tggcagcgtg ggaatgggga cagcttagcg gttttaccac tcgttcgcag
    22081 cgagtatggt tggcggtgtt atgcgggtta ttgttggcgc tgatgctttt tctgttgccg
    22141 gaatatcacc gaaatattca tcaaccgctg gttgaaatct cactttgggc ttcgttgggt
    22201 tggtggattg tcgcgctatt gctggtgctg ttttacccag gttccgcagc aatctggcgt
    22261 aactctaaaa cattgcgcct tatttttggc gtgctaacca ttgttccctt cttctggggc
    22321 atgctggcgt tacgggcctg gcactatgac gagaatcatt acagtggcgc aatatggctg
    22381 ctctatgtca tgatcctggt atggggcgct gactccggcg catatatgtt tggcaaattg
    22441 tttggtaaac ataagctggc accgaaggtt tctccgggta aaacctggca aggctttata
    22501 ggtggactcg ctactgcagc ggtaatctca tggggttatg gcatgtgggc gaatctcgac
    22561 gtcgctcccg tcaccttact catttgctct attgtcgcag cgttagcctc agtgctcggc
    22621 gatctgaccg agagtatgtt taagcgtgaa gcaggaatta aggacagcgg tcatttaatt
    22681 ccaggacacg gtggtatttt agatcgtatt gatagcctga cggctgcggt accggtcttt
    22741 gcttgcttgt tgttactggt attcaggacg ctttaacgga aggtaatatg ctgagttttc
    22801 tctgggattt ggcttcgttc atcgttgcac tgggtgtact tatcaccgtg catgaatttg
    22861 gtcatttctg ggttgcccgg cgttgtggtg ttcgcgttga gcgtttctca atagggtttg
    22921 gtaaggcgct ctggcggcga actgataagc tcggcaccga atatgttatc gccctgatcc
    22981 cgttgggcgg ttatgtcaaa atgctggatg agcgcgcaga accggtcgtt ccggaactcc
    23041 gccaccatgc cttcaataat aaatctgtcg gccaacgagc ggcgattatt gccgcaggtc
    23101 cggttgcaaa cttcattttt gctatctttg cctactggct ggtttttatt attggtgtgc
    23161 ctggcgtacg tccggtggtt ggcgaaatag cagccaattc gatagctgcg gaagcacaaa
    23221 ttgcaccagg tacggaacta aaagccgtag atggtatcga aacgcctgat tgggatgccg
    23281 tgcgtttgca gttggtcgat aaaattggcg atgaaagcac caccattaca gtagcgccat
    23341 ttgacagcga ccaacggcgg gatgtaaagc tcgatttacg tcactgggcg tttgagcctg
    23401 ataaagaaga tccggtatct tcgctgggga ttcgtcctcg tgggccgcaa attgaacctg
    23461 tactggaaaa tgtgcagcca aactcggcgg caagcaaggc aggtttgcaa gcaggcgaca
    23521 ggatcgttaa agtcgatggt cagcccttaa cgcagtgggt gacctttgtg atgcttgtcc
    23581 gggataaccc gggtaaatcc ttagcgttag aaatcgaaag gcaggggagt cccttgtctt
    23641 tgacattaat cccggagagt aaaccgggta atggtaaagc gattggtttt gtcggtattg
    23701 agccgaaagt cattcctttg ccagatgagt ataaagttgt acgccagtat gggccgttca
    23761 acgccatcgt cgaagccacg gacaaaacgt ggcagctgat gaagctgacg gtcagtatgc
    23821 tgggaaaatt gatcaccggt gatgtgaaac tgaacaacct cagtgggccg atctctatcg
    23881 ccaagggggc tgggatgaca gcggaactcg gggtagttta ttacctgccg tttcttgcgc
    23941 ttattagcgt gaacttaggg ataattaacc tgtttccgtt gcccgtactt gacggggggc
    24001 atctgctgtt ccttgcgatc gaaaagatca agggcggacc ggtatccgag cgggttcaag
    24061 acttttgtta tcgcattggc tcgattctgc tggtgctgtt aatggggctt gcacttttca
    24121 atgatttctc tcggttatga gagttagtta ggaagaacgc ataataacga tggcgatgaa
    24181 aaagttgctc atagcgtcgc tgctgtttag cagcgccacc gtatacggtg ctgaagggtt
    24241 cgtagtgaaa gatattcatt tcgaaggcct tcagcgtgtc gccgttggtg cggccctcct
    24301 cagtatgccg gtgcgtacag gcgacacggt taatgatgaa gatatcagta ataccattcg
    24361 cgctctgttt gctaccggca actttgagga tgttcgcgtc cttcgtgatg gtgataccct
    24421 tctggttcag gtaaaagaac gtccgaccat tgccagcatt actttctccg gtaacaaatc
    24481 ggtgaaagat gacatgctga agcaaaacct cgaggcttct ggtgtgcgtg tgggcgaatc
    24541 cctcgatcgc accaccattg ccgatatcga gaaaggtctg gaagacttct actacagcgt
    24601 cggtaaatat agcgccagcg ttaaagctgt cgtgaccccg ctgccgcgca accgtgttga
    24661 cctaaaactg gtgttccagg aaggtgtgtc agctgaaatc cagcaaatta acattgttgg
    24721 taaccatgct ttcaccaccg acgaactgat ctctcatttc caactgcgtg acgaagtgcc
    24781 gtggtggaat gtggtaggcg atcgtaaata ccagaaacag aaactggcgg gcgatcttga
    24841 aaccctgcgc agctactatc tggaccgcgg ttatgcccgt ttcaacatcg actctaccca
    24901 ggtcagtctg acgccagata aaaaaggtat ttacgtcacg gtgaacatca ccgaaggcga
    24961 tcagtacaag ctttctggcg ttgaagtgag cggcaacctt gccgggcact ccgctgaaat
    25021 tgagcaactg actaagatcg agccgggcga gctgtataac ggcactaaag tgaccaaaat
    25081 ggaagatgac attaaaaagc ttctcggtcg ctatggttat gcctatccgc gcgtacagtc
    25141 gatgcctgaa attaacgatg ccgacaaaac cgttaaatta cgcgtgaacg ttgatgcggg
    25201 taaccgtttc tacgtgcgta agatccgttt tgaaggtaac gatacctcga aagatgccgt
    25261 cctgcgtcgc gaaatgcgtc agatggaagg tgcatggttg gggagcgatc tggtcgatca
    25321 gggtaaggag cgtctgaatc gtctgggctt ctttgaaact gtcgataccg atacccaacg
    25381 tgttccgggt agcccggacc aggttgatgt agtctacaag gtgaaagaac gtaacactgg
    25441 tagcttcaac ttcgggattg gttacggtac tgaaagtggc gtgagcttcc aggctggcgt
    25501 gcaacaggat aactggttag gtacaggtta tgctgttggt atcaacggga ccaaaaacga
    25561 ttaccagacc tatgctgaac tgtcggtaac caacccgtac ttcaccgtag atggcgtaag
    25621 cctcggtggt cgtctcttct ataatgactt ccaggcagat gacgccgacc tgtccgacta
    25681 taccaacaag agttatggta cagacgtgac gttgggcttc ccgattaacg aatataactc
    25741 gctgcgtgca ggtctgggtt atgtacataa ctccctgtcc aacatgcagc ctcaggttgc
    25801 gatgtggcgt tatctgtact ctatgggtga acatccgagc acctctgatc aggataacag
    25861 cttcaaaacg gacgacttca cgttcaacta tggttggacc tataacaagc ttgaccgtgg
    25921 ttacttcccg acagatggtt cacgtgtcaa cctgaccggt aaagtgacca ttcctggatc
    25981 ggataacgaa tactacaaag tgacgttaga cacggcgact tatgtgccga tcgatgacga
    26041 tcacaaatgg gttgttctgg gtcgtacccg ctggggttat ggtgatggtt taggcggcaa
    26101 agagatgccg ttctacgaga acttctatgc cggtggttcc agcaccgtgc gtggcttcca
    26161 gtccaatacc attggtccga aagcagttta cttcccgcat caggccagta attatgatcc
    26221 ggactatgat tacgaatgtg cgactcagga cggcgcgaaa gacctgtgta aatcggatga
    26281 tgctgtaggc ggtaacgcca tggcggttgc cagcctcgag ttcatcaccc cgacgccgtt
    26341 tattagcgat aagtatgcta actcggttcg tacttcattc ttctgggata tgggtaccgt
    26401 ttgggataca aactgggatt ccagccaata ttctggatat ccggactata gtgatccaag
    26461 caatatccgt atgtctgcgg gtatcgcatt acaatggatg tccccattgg ggccgttggt
    26521 gttctcctac gcccagccgt tcaaaaagta cgatggagac aaggcagaac agttccagtt
    26581 taacatcggt aaaacctggt aagtgttctc cacaaaggaa tgtagtggta gtgtagcgat
    26641 gactttaggc gatcaatata agatcgccgg gccacgcaaa gaactgcacc ctccggtgca
    26701 aatgggatgg taaggagttt attgtgaaaa agtggttatt agctgcaggt cttggtttag
    26761 cactggcaac ttctgctcag gcggctgaca aaattgcaat cgtcaatatg ggcagcctgt
    26821 tccagcaggt agcgcagaaa accggtgttt ctaacacgct ggaaaatgag ttcaaaggcc
    26881 gtgccagcga actgcagcgt atggaaaccg atctgcaggc taaaatgaaa aaactgcagt
    26941 ccatgaaagc gggcagcgat cgcaccaaac tggaaaaaga cgtgatggct cagcgccaga
    27001 cttttgctca gaaagcgcag gcttttgagc aggatcgcgc acgtcgttcc aacgaagaac
    27061 gcggcaaact ggttactcgt atccagactg ctgtgaaatc cgttgccaac agccaggata
    27121 tcgatctggt tgttgatgca aacgccgttg cttacaacag cagcgatgta aaagacatca
    27181 ctgccgacgt actgaaacag gttaaataag taatgccttc aattcgactg gctgatttag
    27241 cgcagcagtt ggatgcagaa ctacacggtg atggcgatat cgtcatcacc ggcgttgcgt
    27301 ccatgcaatc tgcacaaaca ggtcacatta cgttcatggt taacccaaaa taccgtgagc
    27361 atttaggctt gtgccaggcg tccgcggttg tcatgaccca ggacgatctt cctttcgcga
    27421 aaagtgccgc gctggtagtg aagaatccct acctgactta cgcgcgcatg gcgcaaattt
    27481 tagataccac gccgcagccc gcgcagaaca ttgcacccag tgcggtgatc gacgcgacgg
    27541 cgaagctggg taacaacgta tcgattggcg ctaacgcggt gattgagtcc ggcgttgaac
    27601 tgggcgataa cgtgattatc ggtgccggtt gcttcgtagg taaaaacagc aaaatcggtg
    27661 caggttcgcg tctctgggcg aacgtaacca tttaccatga gatccagatc ggtcagaatt
    27721 gcctgatcca gtccggaaca gtggtaggcg cagacggctt tggttatgcc aacgatcgtg
    27781 gtaactgggt gaagatccca cagattggtc gcgtaattat tggcgatcgc gtggagatcg
    27841 gtgcctgcac aaccatcgat cgcggcgcgc tggatgacac tgttattggc aatggcgtga
    27901 tcattgataa ccagtgccag attgcacata acgtcgtgat tggcgacaat acggcggttg
    27961 ccggtggcgt cattatggcg ggcagcctga aaattggtcg ttactgcatg atcggcggag
    28021 ccagcgtaat caacgggcat atggaaatat gcgacaaagt gacggttacg ggcatgggta
    28081 tggtgatgcg tcccatcact gaaccaggcg tctattcctc aggcattccg ctgcaaccca
    28141 acaaagtctg gcgcaaaacc gctgcactgg tgatgaacat tgatgacatg agcaagcgtc
    28201 tgaaatcgct tgagcgcaag gttaatcaac aagactaacg ttccatcttt tgttcgccaa
    28261 actttacggc ctgtctcatt cttacgattg cggcaggccg tgttattatt gtcgtttctt
    28321 atattttgac aggaagagta tcttgactac taacactcat actctgcaga ttgaagagat
    28381 tttagaactt ctgccgcacc gtttcccgtt cttactggtg gatcgcgtgc tggattttga
    28441 agaaggtcgt tttctgcgcg cagtaaaaaa tgtctctgtc aatgagccat tcttccaggg
    28501 ccatttccct ggaaaaccga ttttcccggg tgtgctgatt ctggaagcaa tggcacaggc
    28561 aacaggtatt ctggcgttta aaagcgtagg aaaactggaa ccgggtgagc tgtactactt
    28621 cgctggtatt gacgaagcgc gcttcaagcg cccggtcgtg cctggcgatc aaatgatcat
    28681 ggaagtcact ttcgaaaaaa cgcgccgcgg cctgacccgt tttaaagggg ttgctctggt
    28741 cgatggtaaa gtagtttgcg aagcaacgat gatgtgtgct cgtagccggg aggcctgata
    28801 cgtgattgat aaatccgcct ttgtgcatcc aaccgccatt gtggaagagg gcgcgtcaat
    28861 tggcgcgaac gcacacattg gtcctttttg tatcgttgga ccccatgtcg aaattggtga
    28921 gggtaccgta ctgaaatctc acgttgtcgt gaatggtcat actaaaattg gccgcgataa
    28981 tgagatttat cagtttgcct ccatcggcga agttaaccag gatttgaaat atgctggcga
    29041 accgacccgt gtggaaatcg gcgatcgtaa ccgcattcgc gaaagcgtca ccattcatcg
    29101 tggcacagtc cagggcggtg gattgacgaa ggtgggcagc gacaacttac tgatgatcaa
    29161 cgcgcacatt gcgcacgatt gtacggtagg taaccgctgt attctcgcca acaacgcaac
    29221 gctggcgggt cacgtatcgg ttgacgactt cgcgatcatc ggcggcatga ccgcagtcca
    29281 tcagttctgc atcattggtg cgcatgtgat ggttggcggc tgctccggtg tggcgcagga
    29341 cgtccctcct tatgttattg cgcagggtaa ccacgcaacg ccgttcggtg tcaatatcga
    29401 agggctgaag cgccgcggat tcagccgtga ggcgattacc gctatccgca atgcgtataa
    29461 gctaatttat cgtagcggta aaacgctcga tgaagtgaaa ccggaaattg ctgaactggc
    29521 ggaaacatat ccggaagtga aagcctttac cgatttcttt gcacgctcaa cgcgcggtct
    29581 gattcgttaa tgactgacca gcgtccatta acgattgccc tggtcgccgg agaaacctcc
    29641 ggcgatatcc tgggggccgg tttaatccgc gctctgaaag aacgtgtgcc caacgcccgc
    29701 tttgttggtg ttgccgggcc acgaatgcag gctgaaggct gcgaagcctg gtacgaaatg
    29761 gaagaactgg cggtgatggg cattgttgaa gtgctcggtc gtctgcgtcg cttactgcat
    29821 attcgtgccg atctgacaaa gcgttttggc gaactgaagc cagatgtttt tgttggtatt
    29881 gatgcgcccg acttcaatat tactcttgaa ggtaacctca aaaagcaggg tatcaaaacc
    29941 attcattacg tcagtccatc cgtctgggcg tggcgacaga aacgcgtttt caaaataggc
    30001 agagccaccg atctggtgct cgcatttctg cctttcgaaa aagcgtttta tgacaaatac
    30061 aatgtaccgt gccgctttat cggtcatacc atggctgatg ccatgccgtt agatccagat
    30121 aaaaatggtg cccgtgatgt gctggggatc ccttacgatg cccactgtct ggcattgttg
    30181 ccgggcagcc gtggtgcaga agtcgaaatg cttagtgccg atttcttgaa aactgcccag
    30241 cttttgcgcc agacatatcc ggatctcgaa atcgtggtgc cgctggtgaa tgccaaacgc
    30301 cgcgagcagt ttgaacgcat caaagctgaa gtcgcgccag acctgtcggt tcatttgctg
    30361 gatggaatgg gccgtgaggc gatggtcgcc agcgatgcgg cactactggc gtcggggacg
    30421 gcagccctgg agtgtatgct ggcgaaatgc ccgatggtgg tgggatatcg catgaagcct
    30481 tttaccttct ggttggcgaa gcggctggtg aaaactgatt atgtctcgct gccaaatctg
    30541 ctggcgggca gagagttagt caaagagtta ttgcaggaag agtgtgagcc gcaaaaactg
    30601 gctgcggcgc tgttaccgct gttggcgaac gggaaaacca gccacgcgat gcacgatacc
    30661 ttccgtgaac tgcatcagca gatccgctgc aatgccgatg agcaggcggc acaagccgtt
    30721 ctggagttag cacaatgatc gaatttgttt atccgcacac gcagctggtt gcgggtgtgg
    30781 atgaagtcgg acgcgggccg ttagttggcg cggtcgtcac cgctgcggtg atccttgacc
    30841 cggcgcgccc gattgccggg ctgaatgatt ccaaaaagct gagcgaaaaa cgccgtctgg
    30901 cgctctatga agagatcaaa gagaaagcgt tgagctggag tctgggccgc gcggaacccc
    30961 acgaaatcga cgagctgaac attcttcatg cgaccatgct ggcgatgcag cgtgccgtcg
    31021 ctgggctgca tattgcgccg gaatatgtgt tgattgatgg taaccgctgc ccgaaattac
    31081 cgatgcctgc gatggctgtg gtgaaagggg atagccgcgt accagaaatc agtgccgcgt
    31141 ctatcctggc gaaagtgacg cgtgacgccg aaatggcggc gctggatatt gttttcccgc
    31201 aatatggttt tgcccaacat aaagggtatc caaccgcttt tcatctggaa aaactggctg
    31261 aatatggcgc gactgaacat catcgtcgca gctttgggcc tgtcaaacgc gcactgggac
    31321 ttgcgtcctg attcttgtgt cgagattaag taaaccggaa tctgaagatg tctgaaccac
    31381 gtttcgtaca cctgcgggtg cacagcgact actcgatgat cgatggcttg gccaaaaccg
    31441 caccgctggt aaaaaaggcg gcggcgttgg gtatgccagc actggcgatc accgatttca
    31501 ccaacctttg cggtctggtg aagttctacg gagcgggaca tggcgcaggg attaagccta
    31561 tcgtcggggc agattttaat gtccagtgcg acctgctggg tgatgagtta acccacctga
    31621 cggtactggc ggcgaacaat accggctatc agaatctggc gttgctgatc tcaaaagcgt
    31681 atcagcgcgg gtacggtgcc gccgggccga tcatcgatcg cgactggctt atcgaattaa
    31741 acgaagggtt gatccttctt tccggcgggc gcatgggcga cgtcggacgc agtcttttgc
    31801 gtggtaacag cgcgctggta gatgagtgtg tcgcgtttta tgaagaacac ttcccggatc
    31861 gctattttct cgagctgatc cgcaccggca ggccggatga agaaagctat ctgcacgcgg
    31921 cggtggaact ggcggaagcg cgcggtttgc ccgtcgtggc gactaacgat gtgcgcttta
    31981 tcgacagcag cgactttgac gcacacgaaa tccgcgtcgc gatccacgac ggctttaccc
    32041 tcgacgatcc taaacgcccg cgtaactatt cgccgcagca atatatgcgt agcgaagagg
    32101 agatgtgtga gctgtttgcc gacatcccag aagcccttgc caacaccgtt gagatcgcca
    32161 aacgctgtaa cgtaactgtg cgtcttggtg aatacttcct gccgcagttc ccgaccgggg
    32221 acatgagcac cgaagattat ctggtcaagc gtgcaaaaga aggcctggaa gagcgtctgg
    32281 catttttatt ccctgacgag gaagaacgtc ttaaacgccg cccggaatat gacgaacgtc
    32341 tggagactga acttcaggtt atcaaccaga tgggcttccc gggctacttc ctcatcgtta
    32401 tggaatttat ccagtggtcg aaagataacg gcgtaccggt agggccaggc cgtggctccg
    32461 gtgcgggttc actggtggcc tacgcgctga aaatcaccga cctcgatccg ctggaatttg
    32521 acctgctgtt cgaacgtttc cttaacccgg aacgtgtctc catgcctgac ttcgacgttg
    32581 acttctgtat ggagaaacgc gatcaggtta tcgaacacgt ggcggacatg tacggtcgtg
    32641 atgcggtatc gcagattatc accttcggta cgatggcggc gaaagcggtg atccgcgacg
    32701 taggccgcgt gctggggcat ccgtacggct ttgtcgaccg tatctcgaaa ctgatcccgc
    32761 ccgatccggg gatgacgctg gcgaaagcgt ttgaagccga gccgcagctg ccggaaatct
    32821 acgaagcgga tgaagaagtt aaggcgctga tcgacatggc gcgcaaactg gaaggggtca
    32881 cccgtaacgc cggtaagcac gccggtgggg tggttatcgc gccgaccaaa attaccgatt
    32941 ttgcgccgct ttattgcgat gaagagggca aacatccggt cacccagttt gataaaagcg
    33001 acgttgaata cgccggactg gtgaagttcg acttccttgg tttgcgtacg ctcaccatca
    33061 tcaactgggc gctggagatg atcaacaagc ggcgggcgaa gaatggcgag ccaccgctgg
    33121 atatcgccgc gatcccgctg gatgacaaga aaagcttcga catgctgcaa cgctcggaaa
    33181 ccacggcggt attccagctt gaatcgcgcg gcatgaagga cctgatcaag cgtctgcaac
    33241 ctgactgctt cgaagatatg atcgcactgg tggcactgtt ccgccctggt ccgttgcagt
    33301 cagggatggt ggataacttt atcgaccgta aacatggtcg tgaagagatc tcctatccgg
    33361 acgtgcagtg gcagcatgaa agcctgaaat cggtactgga gccaacctac ggcattatct
    33421 tgtatcagga acaggtcatg cagattgccc aggtgctttc tggttatacc ctcggtggcg
    33481 cggatatgct gcgccgtgcg atgggtaaga aaaagccgga agagatggct aagcagcgtt
    33541 ctgtatttgc tgaaggtgca gaaaagaacg gaatcaacgc cgaactggcg atgaaaatct
    33601 tcgacctggt ggagaaattc gcgggttacg gatttaacaa atcgcactct gcggcctatg
    33661 ctttggtgtc atatcaaacg ttatggctg
//
LOCUS       DADLTY010000024        33529 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.024, whole genome shotgun sequence.
ACCESSION   DADLTY010000024 DADLTY010000000
VERSION     DADLTY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.024"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            1416..2195
                     /gene="ipaJ"
                     /locus_tag="JN334_000960"
     CDS             1416..2195
                     /gene="ipaJ"
                     /locus_tag="JN334_000960"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HAY7437190.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3142..4071)
                     /locus_tag="JN334_000961"
     CDS             complement(3142..4071)
                     /locus_tag="JN334_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HAY7437191.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4288..4524)
                     /locus_tag="JN334_000962"
     CDS             complement(4288..4524)
                     /locus_tag="JN334_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7437192.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(4528..6429)
                     /gene="ipaA"
                     /locus_tag="JN334_000963"
     CDS             complement(4528..6429)
                     /gene="ipaA"
                     /locus_tag="JN334_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HAY7437193.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6438..7436)
                     /gene="ipaD"
                     /locus_tag="JN334_000964"
     CDS             complement(6438..7436)
                     /gene="ipaD"
                     /locus_tag="JN334_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HAY7437194.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7487..8578)
                     /gene="ipaC"
                     /locus_tag="JN334_000965"
     CDS             complement(7487..8578)
                     /gene="ipaC"
                     /locus_tag="JN334_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HAY7437195.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(8598..10340)
                     /gene="ipaB"
                     /locus_tag="JN334_000966"
     CDS             complement(8598..10340)
                     /gene="ipaB"
                     /locus_tag="JN334_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HAY7437196.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10346..10813)
                     /gene="ipgC"
                     /locus_tag="JN334_000967"
     CDS             complement(10346..10813)
                     /gene="ipgC"
                     /locus_tag="JN334_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocator chaperone
                     IpgC"
                     /protein_id="HAY7437197.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10870..11496)
                     /gene="ipgB"
                     /locus_tag="JN334_000968"
     CDS             complement(10870..11496)
                     /gene="ipgB"
                     /locus_tag="JN334_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgB"
                     /protein_id="HAY7437198.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11512..11901)
                     /gene="ipgA"
                     /locus_tag="JN334_000969"
     CDS             complement(11512..11901)
                     /gene="ipgA"
                     /locus_tag="JN334_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HAY7437199.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11914..13398)
                     /gene="icsB"
                     /locus_tag="JN334_000970"
     CDS             complement(11914..13398)
                     /gene="icsB"
                     /locus_tag="JN334_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HAY7437200.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13712..15328
                     /locus_tag="JN334_000971"
     CDS             13712..15328
                     /locus_tag="JN334_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HAY7437201.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15338..15700
                     /gene="ipgE"
                     /locus_tag="JN334_000972"
     CDS             15338..15700
                     /gene="ipgE"
                     /locus_tag="JN334_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HAY7437202.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15700..16158
                     /gene="ipgF"
                     /locus_tag="JN334_000973"
     CDS             15700..16158
                     /gene="ipgF"
                     /locus_tag="JN334_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgF"
                     /protein_id="HAY7437203.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16167..17282
                     /gene="mxiG"
                     /locus_tag="JN334_000974"
     CDS             16167..17282
                     /gene="mxiG"
                     /locus_tag="JN334_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HAY7437204.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17290..17541
                     /gene="mxiH"
                     /locus_tag="JN334_000975"
     CDS             17290..17541
                     /gene="mxiH"
                     /locus_tag="JN334_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex protein
                     MxiH"
                     /protein_id="HAY7437205.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17554..17847
                     /gene="mxiI"
                     /locus_tag="JN334_000976"
     CDS             17554..17847
                     /gene="mxiI"
                     /locus_tag="JN334_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HAY7437206.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17853..18578
                     /locus_tag="JN334_000977"
     CDS             17853..18578
                     /locus_tag="JN334_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HAY7437207.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18575..19102
                     /locus_tag="JN334_000978"
     CDS             18575..19102
                     /locus_tag="JN334_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HAY7437208.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            19044..19739
                     /locus_tag="JN334_000979"
     CDS             19044..19739
                     /locus_tag="JN334_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MxiN"
                     /protein_id="HAY7437209.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19732..20139
                     /locus_tag="JN334_000980"
     CDS             19732..20139
                     /locus_tag="JN334_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa secretion machinery protein MxiL"
                     /protein_id="HAY7437210.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            20123..20551
                     /gene="mxiM"
                     /locus_tag="JN334_000981"
     CDS             20123..20551
                     /gene="mxiM"
                     /locus_tag="JN334_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HAY7437211.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20681..21313
                     /gene="mxiE"
                     /locus_tag="JN334_000982"
     CDS             20681..21313
                     /gene="mxiE"
                     /locus_tag="JN334_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional activator MxiE"
                     /protein_id="HAY7437212.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21300..23000
                     /gene="mxiD"
                     /locus_tag="JN334_000983"
     CDS             21300..23000
                     /gene="mxiD"
                     /locus_tag="JN334_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HAY7437213.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            23019..24086
                     /gene="mxiC"
                     /locus_tag="JN334_000984"
     CDS             23019..24086
                     /gene="mxiC"
                     /locus_tag="JN334_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HAY7437214.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24099..26159
                     /gene="mxiA"
                     /locus_tag="JN334_000985"
                     /gene_synonym="virH"
     CDS             24099..26159
                     /gene="mxiA"
                     /locus_tag="JN334_000985"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiA"
                     /protein_id="HAY7437215.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26172..26573
                     /gene="spaK"
                     /locus_tag="JN334_000986"
     CDS             26172..26573
                     /gene="spaK"
                     /locus_tag="JN334_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone SpaK"
                     /protein_id="HAY7437216.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            26577..27869
                     /locus_tag="JN334_000987"
     CDS             26577..27869
                     /locus_tag="JN334_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HAY7437217.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27962..28300
                     /gene="spaM"
                     /locus_tag="JN334_000988"
     CDS             27962..28300
                     /gene="spaM"
                     /locus_tag="JN334_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface presentation of antigens protein SpaM"
                     /protein_id="HAY7437218.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28287..29165
                     /gene="spa32"
                     /locus_tag="JN334_000989"
     CDS             28287..29165
                     /gene="spa32"
                     /locus_tag="JN334_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HAY7437219.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSANPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29159..30040
                     /gene="spaO"
                     /locus_tag="JN334_000990"
     CDS             29159..30040
                     /gene="spaO"
                     /locus_tag="JN334_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SpaO"
                     /protein_id="HAY7437220.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            30045..30695
                     /gene="spaP"
                     /locus_tag="JN334_000991"
     CDS             30045..30695
                     /gene="spaP"
                     /locus_tag="JN334_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaP"
                     /protein_id="HAY7437221.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30710..30970
                     /gene="spaQ"
                     /locus_tag="JN334_000992"
     CDS             30710..30970
                     /gene="spaQ"
                     /locus_tag="JN334_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HAY7437222.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30970..31740
                     /gene="spaR"
                     /locus_tag="JN334_000993"
     CDS             30970..31740
                     /gene="spaR"
                     /locus_tag="JN334_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaR"
                     /protein_id="HAY7437223.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQMSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31747..32775
                     /gene="spaS"
                     /locus_tag="JN334_000994"
     CDS             31747..32775
                     /gene="spaS"
                     /locus_tag="JN334_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein SpaS"
                     /protein_id="HAY7437224.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32788..33030
                     /locus_tag="JN334_000995"
     CDS             32788..33030
                     /locus_tag="JN334_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437225.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10455 a         4872 c         6713 g        11489 t
ORIGIN      
        1 ccggctactc attccctgct tgtgcaggat tttaatttcc ataactgtct caaaagtgac
       61 cataagctct cctgaatcag gagagcagat taccccctgg atctgatttc aggcgttggg
      121 tgtggatcac tattgcaccg ttcgtgacac ttgttgacta cctgtgcggc atctggtatc
      181 aaggtggcgt gaagccggtt caggttctca tgcggaacgt tggaacctat atccccccga
      241 taccaaaggc gaaatgtaag tgacaaaatg caatgaacac gttctccgtt aactgaggag
      301 catggtgata aataaaggta aatgcagaag gtgtagagtc tcgcagattt ctgacatcat
      361 agagcctaac attagatgca taacgctaca gattgtagaa tctctacagg tgaaatatac
      421 actggttggc gtttatgtcg caaaacatgt cattcaggtt catctcacca ataaggatat
      481 gagtgaagtg gaggataagt gagtctgctg tcagagtttt ctggtgtatg tcagtaactg
      541 cgaaccatgt ctgcttgata tgggagctta tggaggctct ctgtacaagg ttagggagct
      601 aacaaagcag ggataaaaag tccgcctgat gcaggcacgg ttcgttaaat cttttgccat
      661 tggtaacaaa atggtgcgat gaatgttcag gctatcttta tcttgatggt ggttcagctg
      721 tttggtaaag aaatcgctgt aacaatagaa gaactgcagg cagtactggt cccacatcgt
      781 ttgctcagtc aactggtgaa gttccgtagc gcgtaaatta atgctttgca tgaaacgtta
      841 ctggagtgga tgaaagaata cctgcatata tcattactgc gttggattac cagtacaaca
      901 gattgaatga actggacatg ttgatagacg gcattgaaaa gcagcttata agcatagtgc
      961 ggcagagtta attggagagc acttcgcctt ctgagtatta agtaagcatg gtggtacctg
     1021 cggatactat ctattcactg tgctcggtcg gcagccttac tgaccaagga gttgggggag
     1081 ttgataacaa agttgaaaaa acagctctca accaaggtgg ttattgtaac aatagcaaat
     1141 aagctggtgt gacctgtatc acaataaccg attatggacg taaatacgac aaaaatatgt
     1201 cagtatcagg cggtattaat tgctgatgct gatggacatt aactcttaat aaaagggtgg
     1261 atgctgaaag gttgttatgg tgacggacgt gatgacgacg gaaaagagta tgaggttttg
     1321 gatttaaagt tcgttcgggg aataaggacc cggtcagcta attctcatag tagttcgcat
     1381 tgctggatgc aatactgtgc tgcaattact cgtcgatgtc ggaacaacgg aagccttgca
     1441 aacggggatg tattcataca ggtgttatgt tatatggggt tttattgcag ggggctatac
     1501 caagagaata tatgatatct catcagactg atgtgagggt aaatgagaat agagtaaacg
     1561 aacaaggttg ttttttggcg aggaagcaga tgtatgacaa ttcttgtgga gctgccagtt
     1621 tactctgtgc tgcaaaggaa ttaggggttg ataaaatacc acaatacaaa gggtctatgt
     1681 ccgaaatgac aaggaagagt tctcttgatt tggataatcg ttgtgagcgg gatttatact
     1741 taataactag tggtaattat aatccgagaa tacacaaaga taatatcgcg gatgccggtt
     1801 attctatgcc tgataagata gtcatggcta cgaggcttct tggattgaat gcttatgtcg
     1861 ttgaagaaag taacattttt tcgcaagtaa ttagttttat ttatcccgat gcaagggact
     1921 tattaattgg catgggatgt aatatagtac atcaacgaga tgtgttatct tctaaccaaa
     1981 gagttttgga agctgttgct gtatccttta ttggagttcc tgtcggcctc cactgggtgt
     2041 tgtgtagacc tgatggaagc tatatggatc cagctgtagg agaaaattat tcttgttttt
     2101 caactatgga attaggtgca agaagaagta attctaactt tataggatat actaaaatag
     2161 gtatttccat agttataact aatgaggctt tgtaaattgt cgttttttat ctgtgaagga
     2221 gcttgaatat tttggggtgg ggggcataac aaatgatatt actgagtaca aatttagcta
     2281 tattgggcgg attagttgtg tgggaagacg aaatgtttct cttgaaataa gtgaactccc
     2341 tcaaggaatg attgtgtgct taaatattta agtgctggta agtttgagaa ggctgacata
     2401 ttttgcagag caaacagtga ttgacttgga tgatattttt attttagtca ctcgttgtaa
     2461 ttggagtatg ctttatttta tttgaaaata gatgtatttt atacccgcaa tttaaactgt
     2521 aagccctatg aggggtatgg atattataag ttactctttg accaagcata acttttgggg
     2581 ttagaaaaat aaatctcttg ttaataatta atatgatttt aaaatcaaac ttgtttataa
     2641 ttataaaata atgtaagtcg gtcattttat gggaagatta ggagaataaa acagtaatta
     2701 tatttgagaa atctgctaat atattcttaa agcttttttg atactgtgaa cgaagtctgt
     2761 taatggtgga ttttttgttt atcaccatgt aaaaaaaatt gagtagtaaa agcgttgact
     2821 gctaatcctc gctgttttta ttgaaacgat taaaaatacg ctgaatattt tcggccagtc
     2881 actcatattc acataaaatt ttgaccatca tgccgcatcc cttagttgca caattcattt
     2941 tcaatgatat gaatattact aatttcacca gataattttc tcgtggtcag cagggtatga
     3001 gttttcaccg taattttaag gaaacacagt gcgtggggat tgaataacag atagccgacg
     3061 gaattacccc accggctgaa agtcaagttc aacgaagatt tttattatct gaattgggca
     3121 gtttacatca gtgttcgatg tttatgaaga cgatagatgg cgagaaatta tatcccgaat
     3181 agcttcatct atttctctct gaactgtttt gtttatttta gataatgtaa atattagagt
     3241 tctgtttgtt ttattctctt ttctttttat gtaagtccgt ttgtctttag aaataaataa
     3301 cgagtcaact tttagcgaag ggttttgctt tctgtttttg cttttcttta tgatgtttaa
     3361 aatttctttt ttatatatgt ccgggggcaa atttgtatca aggtctttta tttcttcctt
     3421 taatattggt agtgtagaac taagagattc attagccttt tcaagtcctt tataataatt
     3481 gaataatatc ttgtagtcat taaagttaag ttctgacgcg attggaaata gagatattat
     3541 ttcctgtgga acgcttgctg cctgaaaggc acgagtgact ttcgcgcgag acagattctc
     3601 ttttttggct atgtctttat aggacatccc tgatactttc agaaaattaa gaccaatacc
     3661 aagttctcgg atgctatgct cttttgctgt ttgtatatcg tttgctagtt ttctggcatc
     3721 aagagtagat atatattctt ttgaatatag aacttcaaga tctgctcctg catatattgc
     3781 agatgctctt ctacgagtgc catccagaat ttcaattcta ccatcaatct cccttcctat
     3841 tacagggaag aattgttgta gctttatagt ttttatgata tcggctaatg attcttctgt
     3901 tagggattct tgatcgcgta cattcgtttt ttggttgacg aaggttaaat cttggatcct
     3961 tttatgagga atcttggctt tgataaagga aactttatta ccagaatgga gtgtaaactc
     4021 tttcttttgg ccttccttta tacttaacaa gtcgttgcac aaatccacca tatcacaccc
     4081 tgtttattca tattgattta tattgacaaa agttaaatgc agtgtgagca agattgtatg
     4141 taactgttga tgatagtcaa cggtagtctt aaaaattact attttaatag ctggattgat
     4201 gcaatttatc tttattggat aaaagaataa tattcttgtg gagctctgat gtgagagctc
     4261 catagatgaa aaaatgctta tcaattacta tattgatttt gtactatttt tgactacaga
     4321 aataagatct ccaatagtaa taatgtgttc taatgtagat tcatctatac gtagattgaa
     4381 ctcaaaacta atcatatcca gcatttcaat cagagaaaga gaatcggcag aaagatcttc
     4441 aataaattta gtttcttcag atagtttgct gtacgctata ccatagcaaa aggccactat
     4501 tgataatatt ttttctttta tcatatttta atccttattg atattcttta atacttttga
     4561 tagggaagtg gttacatctt ttgctgcttt atatatatta ttgttttcag ctgtaatatc
     4621 attgttattt tttaaatcag atatatcatc tgtaagtaat tctgcagagg tatcgtcaat
     4681 ttttgacaat accttgctga gggcgctaga tacttctttt gccttttcat agatcgcatg
     4741 atttttatca attgtatctg cgttggtttc tgttacgtag tttcttatat ttgcatctgt
     4801 aacttttaat ttgttgttta ttctatgttc tgttgtggat tttgtaatgt cctttgattt
     4861 ttcctgtaac tctcgttctt gtgttcctga tttagttcca atgagagata taagattact
     4921 tagggagttt gttacttttt ttgaagcttc aaatatcgtt tcccgtgtta aatatgtgtc
     4981 tccatttctt gataaaaaat cagtctgaag tacttttgaa tcagtgtttg agttgaattt
     5041 attgatttgc gaagctactc tatgagaaag agaagcgcta cttttagttg tatctgatga
     5101 gttatcatta ctgcttgtca ctccttccgg gtggagaggg ggggtggttg aagagttctg
     5161 tatgttcaga ggcctgtgtc cccgagaaag agagttattt gcagatcttt cttgactact
     5221 cgtagttaac aggtctgaac ctattccatc cgaattatct ggagtatggt tgaaagcctt
     5281 ttctaaagct gaattatttg caaatgtctg gacagtatct gatatagagt gtggtagaac
     5341 ggaagttact ttttgaacta gtgctctttg tggatttagt aatgagtttc cgttacggga
     5401 taatagatcg tttgtctgag aagtaggaga ctgtgcatca ttttctgggt tctcgtgata
     5461 gctattgtca tatactctgt tgtcaaaaac tctattatcg taagttctat tatcattatt
     5521 tatatgatag tgaattacag gttgggatgt tttctcattg gctccggggg ttggtccgcc
     5581 atctggcata tcttcgggag gtgtagggag gccatcttgg ataggatgcg aatcttttat
     5641 atcttctaaa atatcattaa ggacttttct caacagctca ggaaacacgg ctactgtatt
     5701 taagtctctt gacagtttac ctataccaaa tcctttacta tcaatagtat ttttacgttg
     5761 attgcgcaaa ctatcaaagg caagttttgc ctgtgcgtca actaaatcat ctagtttgag
     5821 agatgaaaca gtttcattac ttaactttaa ttcagcaaga atattattct tgataaatga
     5881 tgatacttca gtattgagga gtttttgtcc caatgataaa gcttcaccag atgtgtgttg
     5941 ttgtgctaat agtgcaagtc tgtgctcaat gttttgtcgt aattgatgga gtttcttatt
     6001 ctttgccgac catggtccaa aggcagaatc ttgggataat ttatctttta taattagttc
     6061 cgaatactgt gcgacaattt tttgaagtcc cttttcgttt tcaaaattaa gaccattttg
     6121 atgtactaag gagtacaaaa catttgaaaa ttcgagaatc tcgcttttgg catacgcatt
     6181 cattgagttg gaataaatgc ttcttaacac atcttctttc cctgaggaag aaaataaaaa
     6241 atcaagacat ttctgattaa aagacgttgc aaagttttct ttttcagaca ctgatgctgg
     6301 tgtttttaga gaagtttgcg aactatggat atcggatatt ttgcttttta actcgctgta
     6361 ttctgttgat gatggtgaag ttgccttata taagaatgtt ggcgcttgag tattatttac
     6421 attatgcatg gcgcacctca gaaatggaga aaaagtttat ctgtatctgt acatgagctt
     6481 attgtactac tcaaaacctt tactaaatta tcaaaaatac tattggcatt actgtatttt
     6541 tgaactaaag tttgaagatt atttttcatt gtttcatctt cggcagagaa tccggcattc
     6601 catgcctgat attttgcatt atctagcaca acctcgccat ttccacctag attatctaag
     6661 ctttttaaca tattgtctat tggggtcatg tttatactga caacatatcc cccgtttttt
     6721 tgagatacct tgccgattgt tccacctaat tctgtaagcc atttatttgc ttgttcctga
     6781 ctaacagtat tatttgctgg atatagcggt ttatctttat atttttcctt gagttcttcc
     6841 aatgcctttt taagcgagtt gacttgtaat ttcacggagt ttccgtcgtt acctccggga
     6901 gagatccagc cggcaagact ggaaagaaca gcgctaaaat cttgatacat ttgagtatat
     6961 gaactaacgg catgttcata tactttcaga tactgttcat taatatcatt gatggagttt
     7021 gcaattttag cccacagttc tctatgagat atcatttgat ctccatcaag ctcggcttct
     7081 ttcggagctg aatgtaataa ttctcttgcg tctttattaa ttggatattc gtgcctggaa
     7141 agaatatcta atagttgagc ggatttattt acatccatgc taatctgaga tgaatgtaat
     7201 gctatttctt ctagcgatgt tttagtcaac gttttttgtg aaagctcttt ctttaatgcc
     7261 tgatttgttg ttctgatatt atgaagggtg tcgttgagca tagtaaggga acttacagga
     7321 tgagaactgg tcgttgtttt tatatcagaa ttaactgttt cggttgatga accgttggta
     7381 ttgtttggac tgaatgatga ggtggaaata ctattagtca gagttgttat attcatggtt
     7441 atttccttac aggaagagcc atatattatt atggctcttg gatatattaa gctcgaatgt
     7501 taccagcaat ctgactggct gtcgaattct ttgattggtt gatgctgtca attatattca
     7561 ataatttttg tattaattga tttgtcgctt gggatgcttc tttagatact tgggatgctt
     7621 cctctgcttg tttactgctg gcctgactga ttagttgttc ttcttcttca agagcagatg
     7681 cataacgccc tcctgatgtg gatatattac cagcaacggc tgatgtttcc attgttgcac
     7741 ggccaatgtt ttgtttttgc tgcgcagaaa gggtattgag ctcgtaagtt cttctctggg
     7801 tgtcaatttt atcctgcaaa gaaatatcgg gagaactaag agaagtttta tgttcagttg
     7861 acagggatat attatctggc gccagtttat ttttacctaa aaattttgtg ctagagttag
     7921 tttgtggtga ggtgatattt gtatctattt gtttatttaa ccctaattta gaacctgcaa
     7981 gctctttttc aagagattga gcagtggcaa ggttctttct taaggctcct ttttggtcgc
     8041 taatccctga atgcgttttt ttggcaccga tacccgttat acctacttgt gtgactgctc
     8101 cagtaatgct tgatgatagg gctgccaggc cttgccgaac aatgttctct gcagctgatt
     8161 ttgtagcatc gaacgcaatc aatgacaatt gagagcccaa tttagtttct gcagtgcgga
     8221 gagcagaaag tagaacggct acactaataa ttaaagaaac agcattagaa gaaagactgg
     8281 aaatatccag agtgttctct ggagtaaggg tgtgactcat ctctgaaaga tctttcatct
     8341 ttttgtcagt aaggcgagca atgatttctg aatcatgctg aacctgctct gataacttta
     8401 aaagttgatc atcatttaat gtggttgtta atacgggatt tttaccgaca ttaagtggaa
     8461 tatgcgctgc aatctgctga taattttgtg atttttgtgt ttcggaagaa ctttgagttt
     8521 gttttgtgga tatatctgta tataaaatct gggttggttt tgtgttttga atttccataa
     8581 cattctcctt atttgtatca agcagtagtt tgttgcaaaa ttgcttttgc aacatcagtt
     8641 ctattagcct gagagttgga cattgaggct aatagatctg caattacttc ctgcaattgg
     8701 ccgaattttt ctattgcttc actgatatat tttgacagtt gttcaacttg atatttcgat
     8761 aatgtcaggt ctgctagatt tgtcgacgcg ctgttctgga aaacagcaga agcgacactt
     8821 cctcccgctt gtgtggcaga gttaacactt tctcctaata aaactgcttg gtttaaatgg
     8881 gtggaaataa tttgtttgga tattacttca tcacccgctg caccaagaaa tttatttaat
     8941 ctggcaacag cattagtgat taaatcgtcc agttgagaag aaaaattctt gagaaacttt
     9001 ggtataaggt ctgtgagggt tttacctatt attttgccaa tattttctgc aagttttgct
     9061 gctgcctgtt taccaacagt ggctacgaga acgactgctg caactaggac aagagcgcct
     9121 gcgattgccc ccagaataga gccaatcatt ttggcttttt tcgagtcgac gcccaagcct
     9181 tcgagcattt ttgtaaatgc atctgaaagg agtttgatta agggttcaat gactgctttc
     9241 atgatcggat tcagggcttg ttccatgaag gaattgccgg tcgctgcttg tactatagca
     9301 tccgtaacca taagagctaa accaacagct gccagtgcta gagaggctcc tccagaaaaa
     9361 gctgctgcaa caacactaac gatagttaaa agtgccccaa gtattttccc aacacaaccc
     9421 attactctgt tgagttcttc tgctttacgt acttcagcag catactcatc agattttctc
     9481 tccatttcag tttttcttga ttcttggaga gactggaata gagccagatc attttttaaa
     9541 gattcttcat tattttttcc aactagttga ataaaggttg ccatcaattg agtaacactg
     9601 gcaagtccgg ttaatgattt ctgctgggtt gatagctgtt cagcagatgc tgtgtttgaa
     9661 aatgcagaaa aagagtctat ttctttttcg agttgcattg atttatctgt aagtttgcta
     9721 tgaattgaca gggttttctg ctcaatcaat gtcctgtctt taactgctgc gtcttttttg
     9781 atagtgagtt gtatttcttc ccggcttaat tttttctttt ctggtgactc tgggtcgagt
     9841 tcggataatc ttgtttgaat ttggttaatt ttattttcta ggtcttttat tttagaatct
     9901 gcgtttttta gtttattaat ttgtttttca tagtctctgg ttagtccttc agtttcagat
     9961 agaagagtgt taattttatc ggagaattct aggttttttt gctgtcttgc ctgttgctgg
    10021 gacttccaag cagtaatttt atttgttaat gcagttaaag atttttcacc gagtatttga
    10081 ataaggtttc caattaaaag cgttagttgg gaacttgcat ttaatgactt tggtgcttta
    10141 agttcaggga ttaatatatt tgaagttgag tgtgcattag ttgtattaat attttggtta
    10201 gcagtaagat cagcaattgt aagagaaaat aatttgttag ctgcatcatt tgcagcttgg
    10261 atagtattgt ctccaagctc agtggaagcc aatattttgg caagaggaaa accagtggtt
    10321 gtggtgctta cattatgcat aataattact ccttgatatc ctgaattgcg tccaagtatg
    10381 attgtgcttt tatttttaat ttttcatcat tgctgtgttg aattacgagt tcgaagcact
    10441 ctttagcttt taagggggct ttcaaccgaa gctggcattg tccagtatgg aatactggtg
    10501 tatagtcatt ttttcctaat gcaaaagcga cagcataaag gtctgctgct tgttggaact
    10561 gttcttttat ctgataaata gctgcgagtc ccataatgta gtctacattg taaaagtcgt
    10621 atatacataa aaacctgaag aaaacttcag cttcctctat tcttcctttg ttgtaaaagt
    10681 cataagcata tgaataaatg tcatccatca tatcatcagg aattgcatta atatctttca
    10741 gtgtagcgcc agagttaatt gcatcaatta ctgcagtaga gatgctttca ttttcggtga
    10801 tatttaaaga cataaggtct cctttttgtc tgctttactt taataagtat aagatttaat
    10861 ataaaagatt taatttgtat tgctttgacg gtatacagcc tttagaacct ctgcgcttat
    10921 ttctatttgt aaaacttttg ttgattcatt aaatatttta tcgtagacgg aactggatac
    10981 tagatttgta agatccttca tttgtgtgtt aaacaaatcc atgtcactgc tatagcgttt
    11041 taaaatcttt gctctgaggc cagatgaggc aatctcgttt agtgctgtat gactgataga
    11101 tgtttgtgct gatttttcat caagttttat atccgggaat tttttttcaa tagctgcaaa
    11161 tatgtttttt ttgttatctg aggtcaaaac aactccattg tgtatggcca tttcatctat
    11221 acttctgttt atcattgaag agacataagt ggttcgctct tggctcatcc aacaaaatag
    11281 ttgattcccc gaattactat cttttatttt gtctatgacg ctgtccctga ttttatttat
    11341 attcaccggg catccaaaat tcttctgagg aaagcgttgt tttaagttaa aaacagataa
    11401 tattttcttt acaagcttat tgcgacttaa cgaatcaaat gatgggcgag gaatcgcgaa
    11461 ttcaacctgt ggaagtattt tgtttagaat ttgcatgata ccccctatat gttagttcac
    11521 ttctgaagtg atgtttgcaa tggttagttt aatacaaaat aaaaattcat acaaagattc
    11581 aatgagttca tcaggtgaaa gatatttttt gtagtgtttc ttggtcaaaa cacattgatg
    11641 agtatcaggg agaagacaga tagaagaacc ataatctcta ctcaaagagt agttaaggat
    11701 gagtatgtgt tctggattaa taatattttc tgtaaaaagt tcatgttcat taagaagtcc
    11761 ggtcaaaaag atgtattcat cattatctgt tatacaaaca ggtatttgtt cttcgagcaa
    11821 tataaaggct tgatttttat cgttaaaatc aagctttgca cctgttattt cataaagttt
    11881 atcatatagt ttgcgacaca tttctatggc cttctatata ttagaatgag agttattcaa
    11941 taaaagctta ttgatctgta ataagtcgtg aatgtaaagt ttggttccaa gatctggcga
    12001 tttaagagaa ttgtaataat caataatttt tattgatttt tcgatgattt cttctttttt
    12061 atctgttgct tcggttttta attcgtttaa tacacgagtc atattgctgg gtgctgattt
    12121 tactaagtat gagtggcaag cgttgatgac cgcatcaaag tcttctgaag atacattttc
    12181 agtaggatgt ttgtaaagtg aaattgataa aggcttgaat gtttttggtg tgtggataag
    12241 gttaatttta ttgaaacttt taagatagct gcgtcgtaat tgagtcaatc tggaaagatc
    12301 aaaatcagat tcaatatact tcattatttc gttataagac tgattgagac tttcaatgcg
    12361 ttgcctaact ttatttgcat acgcgtatac atcgttaggt gtagcaacca atttaacgtc
    12421 agggagtgga aagtatattt cagaatttcc tgctttaaga acattaagtg ccataccagc
    12481 acagttttca gatttactga ttaatttata atttgccaat tgtctgaaag catctgcttt
    12541 ctttttgcat ataaattttg acatgtttgt ttcatcaagg ccaaaaaggt tatggctgat
    12601 tttaccatcc tttgttttat tatcacctga taatggaatg tatactttat cagcactgac
    12661 cccccaataa ccataggggt cttttttttg attagctctg ggaaaaaata tttttttgtt
    12721 gttattttga tcttgttttg aatttaataa gtttctaacc tcttggcctg catcaagtct
    12781 ttcggctgtt tggtctgaaa tcatatttag tttgtcttgc ttataggtat cgacactgta
    12841 acctgagcgt ttttcaaaaa ggtagtttat ttctgtatct tttgtaacat tcccgccggg
    12901 ataccagctg agataggtat gagttggttc ctgtagtaaa ttgtttttta tactgatact
    12961 agtatgccct aaatgggtta tgcctctatt tccccagcaa cgaaaaacta aatgtctttc
    13021 aagatttttg gctgtaccga tgccatgaaa acgctctgcg gtattcaaca ttaatttgat
    13081 gtctctacct gtgaaagagt gttgaattga gtttttagct gcttcatagg cggcaaacgc
    13141 actttctgca gatttttcgc tagctaatgt attgaggaaa agcaataagg atttttgatt
    13201 ctctagtcga taatttttta gttgttcatt taatatctgt gaatttatct ttgcattaat
    13261 agtgcttaat gttctgaaaa agagatcctt taggttgaat ttctgtgcta tcaatatagg
    13321 tccatgctct gtatcttcaa cgcgtatagg cccttttgta ttgcttgcgt caatgaaatt
    13381 gctaattttg aggatcatac tttattaact ctccattact tggtgattgt ggggatgtaa
    13441 aatgtatttg gtatataata tattcaatta ataaatttag aaacttgagc ctgttaacat
    13501 aatcaaattt tcttttgctg tacataatat gtacctcgtg agcatatgta gtgctcgttt
    13561 catcatgaaa tcccacaaga taaagtgcct gatgtatcag gctcggagtg ttatagaaaa
    13621 agagagaacc ctgttgaata agtatctttt ttgttgtaaa acgaggtaca aaactgcaat
    13681 caatgcccca tttatgactg aggataatta aatgcacata actaatttgg gattgcatca
    13741 ggtttcattt caaagcggag attcctataa aggcgcagaa gaaaccggaa agcacaaagg
    13801 tgtaagcgtg atttcatatc aaagagttaa aaatggagaa agaaataagg ggatcgaagc
    13861 gcttaatcga ttatatttac agaaccaaac aagcttaaca ggaaaaagcc ttttgtttgc
    13921 gcgtgacaga gctgaagttt tttgcgaggc aataaagttg gcaggtggtg atacgtcaaa
    13981 aattaaagcc atgatggaac gattagatac ctataaactt ggtgaagtta ataaaagaca
    14041 tattaatgag cttaataaag taataagtga agagataaga gcacagctag gcattaaaaa
    14101 taaaaaggaa ttgcaaacga aaataaaaca aatatttact gattatttaa ataataagaa
    14161 ttggggaccg gtcgacaaga atattagtca tcatgggaaa aattatggtt ttcaattaac
    14221 tcctgcctct catatgaaga tagggaataa aaatatattt gtcaaagagt ataatggaaa
    14281 aggaatttgt tgtgcttcta cgagagagag tgatcatatc gcgaatatgt ggctttcaaa
    14341 agtggtggat gatgagggga aggaaatttt ttcgggtatc agacatggag ttatatcagc
    14401 ctatggattg aaaaaaaact catctgaaag agctgttgct gctcgtaata aagctgagga
    14461 gttagtaagc gcagcattat atagcagacc agagttatta tcacaggctt tgtctggtaa
    14521 aacagtagat ttaaagattg tttcgacttc tcttctgacg ccgaccagtt taaccggggg
    14581 agaggagagt atgttaaaag atcaggtgaa cgcattaaaa gggttaaact ctaaaagggg
    14641 gaagccaacg aaattattaa ttcggaatag tgatggtctt ctgaaagaag taagcgttaa
    14701 tctaaaagtg gtgacattta actttggtgt aaatgaatta gcgctaaaaa tgggcttagg
    14761 ctggaggaat gttgacaagc ttaatgatga atcaatatgt tctttgttgg gggataattt
    14821 cctcaaaaat ggtgtgattg gaggttgggc tgcagaagca atagaaaaaa atccaccatg
    14881 taagaatgat gtgatatatc tggctaacca gataaaagag attgtaacca aaaagttaca
    14941 aaaaaatgat aatggagagc cgtataaatt gtcacaaaga atgactctat tggcttatac
    15001 tataggtgct gtaccttgct ggaattgtaa gagtgggaag gacagaacag gtatgcagga
    15061 tgctgaaatt aaaagagaaa taataagaaa acatgaaaca ggtcagtttt ctcaattaaa
    15121 tagtaaatta tcctcagaag agaaaagatt attttctact attctaatga atagtggtaa
    15181 tatggaaatc caagagatga atactggtgt gcccggaaat aaagtcatga aaaaattgcc
    15241 gctatcttcg ttagagctat cttattctga aagaataggg gacccaaaaa tatggaatat
    15301 ggtgaaaggg tattcgtcat ttgtataaga ggaatatatg gaagatttag cagatgttat
    15361 ttgccgagcc ttgggtatcc ctttaattga tatcgatgat caagcaatca tgcttgatga
    15421 tgatgtgcta atatatattg aaaaagaagg agattcaata aatttattat gccctttttg
    15481 tgcattgcct gaaaatataa atgatcttat atatgcgttg agcctaaatt actcagaaaa
    15541 gatatgctta gctactgatg atgaaggcgg aaatctaatt gcgcgattag atcttaccgg
    15601 gataaatgaa ttcgaggatg tatatgttaa tacagaatat tatatttcgc gtgtgcgttg
    15661 gctgaaggat gaatttgcgc gaagaatgaa ggggtattaa tgtcccgttt cgtatttatt
    15721 ttgctgtgtt ttatcccata tttagggaga gctgattgtt gggataaggc tggtgaaagg
    15781 tacaatattc catcaagttt gttaaaagcg attgcggaaa aagaatccgg atttaataaa
    15841 tctgcagtca atgttaataa caatggaagt aaagattatg gtataatgca aataaatgac
    15901 tttcattcta aaagacttag agaaatggga tattctgagg aaatgctaat tagtcatcca
    15961 tgtctttctg tacattatgc agcaaagtta ttaaatgagt ttatgatgat gtatggaaga
    16021 gggtgggagg ctgttggagc ctataatgca ggaacgtctc cgaaaaagaa aaaagaaaga
    16081 ttaaagtacg cagaggatat ctatagacgc tatttgcgga tagcagcaga gagcaagcag
    16141 aataatcgaa ggatataaga ggatttatgt ctgaggcaaa gaactcaaat cttgcaccat
    16201 tcaggttatt agtgaaactc acaaacggtg tcggggatga gtttccgtta tattatggga
    16261 ataacttaat tgttctcgga cggacgatag aaacacttga gtttggaaac gacaatttcc
    16321 cagaaaatat tattcctgta acagatagta aatcggatgg tataatttat ttgaccataa
    16381 gcaaagataa tatctgtcaa tttagtgatg aaaaaggtga gcaaattgat atcaattcac
    16441 aatttaattc atttgaatat gacgggattt cttttcacct taaaaatatg agggaggata
    16501 aatcgagagg ccatatacta aacggaatgt ataagaacca ctcggtgttt tttttctttg
    16561 ctgttattgt tgtgttaatt ataatttttt ccctctcttt aaagaaagat gaagtaaaag
    16621 aaattgctga aattatagat gataagagat atggaatagt caacactgga cagtgcaatt
    16681 atattcttgc cgaaacacaa aatgatgcgg tatgggcgtc tgtagcctta aataaaaccg
    16741 gatttaccaa atgcagatat attctggtgt caaacaaaga aataaataga attcaacaat
    16801 atatcaatca acgattccca tttatcaatt tgtatgtgtt gaatttagtg agtgataaag
    16861 cggaattact ggtgttttta agtaaagaaa gaaatagttc aaaagatacc gaacttgata
    16921 aattgaaaaa tgctctgatt gttgaatttc cgtatatcaa aaatattaaa ttcaactatc
    16981 ttagcgatca taatgcaagg ggggatgcta aaggtatttt cacaaaggta aatgtccagt
    17041 ataaagagat ttgtgaaaat aataaagtta catattctgt cagagaagaa ctgacagatg
    17101 aaaagcttga gctcattaat agactgattt ctgaacataa aaatatatat ggagatcagt
    17161 atattgagtt ttctgtactt ttgatagatg atgattttaa aggtaaatca tatcttaaca
    17221 gcaaagacag ttatgtaatg ttgaatgata aacactggtt ttttttagat aaaaataagt
    17281 gaggataaaa tgagtgttac agtaccgaat gatgattgga cattgagttc attatctgaa
    17341 acttttgatg atggaactca aacattacaa ggtgaactaa cattggcact agataaatta
    17401 gctaaaaatc cttcgaatcc acagttgctg gctgaatacc aaagtaaatt atctgaatat
    17461 acattatata ggaacgcgca atccaataca gtgaaagtga ttaaggatgt tgatgctgca
    17521 attattcaaa acttcagata atagggagca ttcatgaatt acatttatcc agtcaatcag
    17581 gttgatatta tcaaagccag tgattttcaa tctcaagaga tatcaagtct ggaagacgtc
    17641 gtgtcggcta aatatagtga tattaagatg gatacagata ttcaagtatc acaaataatg
    17701 gagatggtaa gcaatccaga atcattaaac ccagaatctt tggccaagtt acagacgacg
    17761 ctctcaaatt attcaatagg agtatcatta gctggcacgt tagcaagaaa aacagtttcg
    17821 gctgttgaaa ctttattaaa gtcttaattt atatgattag gtataaaggt tttattttat
    17881 tcttgttgct gatgttgatt ggatgtgagc aacgtgaaga gttaatttct aatttatctc
    17941 aaagacaggc aaatgaaata atatctgtgc tagaacgcca taatattact gctagaaaag
    18001 ttgatggagg taaacagggg atctcggtac aagtcgaaaa ggggacattt gcatcggcag
    18061 ttgatttgat gcgcatgtac gatttgccaa atccggagag agttgatatc tcacaaatgt
    18121 ttcctacaga ttcattagtg tcttctccaa gagctgaaaa ggcccgttta tatagtgcta
    18181 ttgagcaacg gctggaacag tctttagttt ctattggtgg tgttatttcg gcaaaaatac
    18241 atgttagcta tgatcttgaa gaaaaaaata tatcttcaaa accgatgcat atatcagtaa
    18301 tcgctatata tgactcaccg aaagagtctg aactattagt tagtaatatt aagcgatttt
    18361 tgaaaaacac cttttctgat gttaagtatg aaaatatatc tgtcatatta actccgaaag
    18421 aagaatatgt ttatacaaat gtacaacctg ttaaggaagt taaatcggaa tttttaacaa
    18481 atgaagtaat atatttattt ctcgggatgg ctgtactagt tgtcattctt ttggtatggg
    18541 cattcaaaac agggtggttc aagagaaaca aaatatgata agaatggatg gaatttataa
    18601 aaaatatctt tcaataattt ttgatccagc gttctatata aatagaaatc ggttgaattt
    18661 gccttctgaa ctgttagaaa atggcgtaat cagaagtgag attaataatc tcataattaa
    18721 taaatatgat ctaaattgcg atattgaacc tttaagcggg gtaaccgcta tgtttgttgc
    18781 caactggaat ttacttccag ctgttgctta ttttatcggt agtcaagagt ctcggttaat
    18841 taatcatagt gagatggtta ttagttatta tgggggcaaa atcagtaagc aaggggaggc
    18901 cgctattcga agtggttttt ggcatcttat tgcgtggaag gaaaatatat ctgttggtat
    18961 atatgaaaga ataaacttat tatttaatcc aattgctttg gagggaaatt atacgcctgt
    19021 agaaagaaat ttgtcccgat taaatgaagg tatgcaatat gcaaaaaggc actttaccgg
    19081 tatccagaca tcatgcctat gatggtgttg ttataaagag aattgaaaag gaattatgta
    19141 aaaccataaa agatagagac actgagagta agaagaaagc aatttgtgtt ataaaggagg
    19201 ctacgaaaaa agctgaatca ctgcgtatag atgctgtatg tgatggttat caaattggta
    19261 tacagactgc ttttgagcat attattgact atatttgtga atggaagtta aagcaaaatg
    19321 aaaatagaag aaatattgag gactatataa ctagtttgct ttcagaaaat ttacatgatg
    19381 agaggatcat tagcacttta ttggagcagt ggcttagttc gttgcgtaat acagtaacag
    19441 aattaaaagt ggttttgccc aagtgtaatt tagcactgag gaaaaagtta gaacttgatc
    19501 tccataaata tcgctcagat gtcaagatta ttttaaaata ttctgaaggg aataattata
    19561 tattctgtag tggtaatcaa gtagtggagt ttagtcctca ggatgtaata tcaggtgtta
    19621 aaatagaact tgcggaaaaa ttaacaaaaa atgataaaaa atatttcaaa gagttagcac
    19681 ataagaaact gagacaaatt gcagaagact tgttaaaaga gaatcctgtt aatgattaat
    19741 caaataaatg caagcaatgc tctgcagcaa agattaaata gtgaagaagt tgttaattta
    19801 aatgagagat tatcatcttc gcaatcattt gatgaggata taatctatga aataatgcaa
    19861 tatttctcac agagtgaatt aaattccata gataatgatg aactgcacaa taagattgaa
    19921 caattattca attcacgttt tccttatctt acagctgcac aaaaaagttc acttcttaat
    19981 aaactcatag atgcaaatca atatgtcgat ttgcatgagg gattttatgc atcattgtcc
    20041 atatataaca atattgattt ctatattaag actacaacct tcgattcatt aattagtgtc
    20101 tttgaagcag ggagagaggc agatgattcg acatggtagt aataagttga aaatatttat
    20161 tttaagtata ttgctattaa cactgagtgg gtgtgcttta aagtcatcat ctaattctga
    20221 aaaagaatgg catattgttc ctgtaagtaa ggattatttt tctattccaa atgatttatt
    20281 atggtcgttt aatacaacca ataaaagtat aaatgtttac tctaaatgta ttagtggtaa
    20341 ggcggtttat agttttaatg caggtaaatt catgggcaac tttaatgtta aggaagtaga
    20401 tgggtgcttc atggatgcac aaaagatagc tatagataaa ctattttcta tgctgaaaga
    20461 cggggttgtt ttaaaaggta ataagataaa tgataccatc cttatagaga aggatgggga
    20521 agttaaatta aaattaattc gagggatata attgtattgt gagtaaatat aaaggtctaa
    20581 atacaagtaa tatgttttac atttactcta gtggacatga accagttaac gttgagcttg
    20641 taaaagataa agaacgtaac ataattgagc tggctccagc atggaagggt ttttttttgt
    20701 ccgaaatcaa aatataaaat tcagtgataa tgtgaactat cattatcgct ttaacattaa
    20761 tagttgtgca aaatttcttg ctttttggga ctatttcagt ggtgctctag tagagcatag
    20821 ccatgctgaa aaatgtattc atttctatca tgagaatgat cttcgggata gttgtaatac
    20881 cgaatcgatg cttgataagt taatgttaag attcatcttt tcatctgacc agaatgtaag
    20941 taatgcactg gctatgatac gaatgactga gtcatatcat ttggttcttt atcttctgag
    21001 aactattgag aaagaaaagg aggtaagaat aaagagtctg acagagcatt atggtgtgtc
    21061 ggaggcttat tttagatctt tatgtagaaa agctttaggg gcaaaagtca aagaacaact
    21121 gaacacttgg cggttagtaa atggtttatt agatgtattt ttgcacaatc aaacaatcac
    21181 ttcagctgcc atgaataatg ggtatgcatc aacatcccat ttttcaaatg agataaaaac
    21241 acgactaggc ttttctgcaa gagaattatc gaatattact tttttagtga aaaaaataaa
    21301 tgaaaaaatt taatattaaa tctttgactc tcttgattgt attgttacca ttgatagtta
    21361 atgctaataa tattgactct catttattag aacaaaatga catagcgaaa tatgttgctc
    21421 aaagtgatac ggtaggatct ttttttgaac gtttttctgc gttattaaat tatcctattg
    21481 ttgttagcaa acaagctgca aaaaagagaa tatctgggga gtttgattta tctaatccag
    21541 aagaaatgct tgaaaaactg acattacttg tgggactcat atggtataaa gatggaaatg
    21601 cattatacat atatgactct ggagaattaa taagcaaagt tattttgctg gagaatatat
    21661 cacttaatta tcttattcag tatctgaagg atgctaacct ttatgatcac agatatccta
    21721 taagaggaaa cattagtgat aaaacattct atatatctgg tcctccggca ttggttgaac
    21781 tggtagctaa tacagcaact ttgcttgata agcaggtttc ttctattggg acagataaag
    21841 ttaattttgg ggttataaaa ttaaaaaata catttgttag tgacagaact tataatatga
    21901 gaggggagga tattgtcatc ccaggggtgg ctactgttgt agaacgttta ttaaataatg
    21961 gtaaggctct gagtaacaga caagcccaaa atgatccgat gccacccttt aatattacac
    22021 agaaagtatc tgaggatagt aatgactttt catttagttc agttaccaat agttctatat
    22081 tagaggatgt atcacttatt gcatatccag aaaccaattc tatattagtc aaaggtaatg
    22141 atcagcaaat acagattatt cgcgatatta tcactcagtt agatatagcc aaacgacata
    22201 tagaattatc attatggata atcgatattg ataaatctga attaaataac cttggtgtta
    22261 attggcaagg aacggcttca tttggtgatt cgtttggtgc gtcatttaat atgagctcat
    22321 cagcatcgat aagcactttg gacggcaata agtttattgc ttcagttatg gcattaaatc
    22381 agaaaaagaa agcaaatgta gtttcaaggc cggtgatttt gacacaagag aatattccgg
    22441 ctatatttga taataacagg actttttatg tgtcattagt tggtgagcgc aattcttcgt
    22501 tggagcatgt tacgtatgga acattgataa atgttatacc tcgcttttca tctcggggac
    22561 aaattgagat gtctttaacg atagaggatg gaacaggaaa tagtcagtca aattacaatt
    22621 ataataatga aaatacatct gtccttccag aagttggaag gaccaaaatc agtacaatag
    22681 cgagagttcc acaaggcaaa agtttgttga taggtggtta tacgcatgaa acgaactcta
    22741 acgaaataat atctattcct tttctctcct ctattcctgt aatcggtaat gtgttcaagt
    22801 ataaaacaag taatatcagt aatattgtaa gggtttttct gattcaaccc cgtgagataa
    22861 aggagtcaag ttattataat actgcagaat ataaaagcct catatctgaa agagaaatac
    22921 aaaagactac acaaataatc ccatctgaaa ctactttgct ggaagacgaa aaatcattgg
    22981 tttcatactt aaattactaa ctataaagta ggtgatgtat gcttgatgtt aaaaatacag
    23041 gagtttttag ctctgcattc attgataagt tgaatgcaat gacaaattca gatgatggag
    23101 atgagactgc tgatgcagag cttgattctg gcttggctaa tagcaagtat attgactcat
    23161 ctgatgagat ggcttccgct ctttcgtcat ttataaacag aagagacctt gagaaactga
    23221 aaggaacaaa tagtgatagt caggaacgta ttttagatgg ggaagaagat gaaattaatc
    23281 acaagatttt tgatttaaag agaacgttaa aagataacct tcctctagat cgggatttta
    23341 tagacagact aaagagatat tttaaagatc caagtgatca agtcttagca ttaagggaac
    23401 ttttgaatga aaaagatctt actgctgaac aagtcgaatt attaactaaa attattaatg
    23461 agataatatc aggtagtgaa aaaagtgtta atgctggaat aaattcagct atacaggcta
    23521 aattatttgg caacaaaatg aaacttgaac cacagctttt gcgtgcatgt tatcgtggtt
    23581 ttatcatggg gaacatatca acaacagatc agtatataga atggcttggt aattttggtt
    23641 ttaatcacag acatacaatt gtgaattttg tagagcagtc actgattgta gacatggatt
    23701 ctgagaaacc gagctgtaat gcttatgagt ttggttttgt gttatctaaa ttaattgcaa
    23761 ttaagatgat tagaacttca gacgtaattt ttatgaagaa actggaatcc tcaagcttgc
    23821 taaaagatgg cagtttaagt gcagagcagc tattgctaac tttattatat atttttcaat
    23881 atccaagtga aagtgagcaa attcttactt ctgttataga agtatcacga gccagtcatg
    23941 aggattctgt agtgtatcaa acatatctat cttctgttaa tgaaagtcct catgatatat
    24001 ttaaaagtga aagtgaaaga gaaattgcga tcaatattct acgagagctt gtcacaagtg
    24061 catacaagaa agagctttct agataacagg agataaaagt gatccagtct tttcttaagc
    24121 aagtaagtac taagcctgaa ttaattatat tagtgcttat ggttatgatc attgctatgt
    24181 tgatcatacc attacctaca taccttgttg attttttaat agggcttaat attgtacttg
    24241 ctattctcgt ttttatggga tcattttata ttgaaagaat attgagtttt tctacattcc
    24301 cttcggtttt gttgataacg acattatttc gtctcgcttt atcaataagt actagtcgac
    24361 taattttagt tgatgcggat gcgggtaaaa ttattactac atttggccag tttgtcattg
    24421 gtgatagtct ggctgtaggt tttgttattt tttcaatagt aactgttgtg cagttcattg
    24481 ttataacaaa aggatctgaa agggttgcgg aggtagctgc tcgcttttct cttgacggta
    24541 tgccggggaa acaaatgagc attgatgcgg atttaaaggc tggaattatt gatgccgcag
    24601 gagctaaaga aagacgcagt attctggaac gtgagagtca attgtatggt tcatttgatg
    24661 gagcaatgaa gttcattaaa ggtgatgcaa ttgctggcat cattattatt tttgtcaatt
    24721 taataggagg tatttctgtt ggtatgagtc aacatggaat gtccctatct ggtgcattat
    24781 ctacttatac catactaact attggtgacg gattggtctc tcagattcct gctctgttaa
    24841 tttctataag tgcaggattt atcgttacaa gagttaatgg tgatagcgat aatatgggac
    24901 gtaacataat gtcccagata tttgggaatc cttttgttct cattgttaca tcagctcttg
    24961 ccttggcgat agggatgttg ccaggttttc cgttttttgt ttttttcctg atagcagtta
    25021 ctttgacggc tttattctat tataaaaagg tcgtagaaaa agaaaaaagt ctgtctgagt
    25081 ctgattctag tggctatact ggtacatttg atattgataa tagccatgac tcatctttgg
    25141 caatgataga aaatctggat gctattagtt cagaaaccgt tcctttaatt ttattatttg
    25201 ccgaaaataa gataaatgca aatgatatgg aaggtcttat tgaaaggata agaagtcagt
    25261 tctttattga ttatggtgtc aggcttccaa ctattttata tagaacaagc aatgagctta
    25321 aggtcgatga tattgttttg ttaataaatg aggtgcgtgc cgatagtttt aatatatatt
    25381 ttgacaaagt gtgtattaca gatgaaaatg gagatataga tgctctaggt attcctgttg
    25441 tttcaacttc atataatgaa cgtgttattt cttgggtaga cgtttcatat acagaaaatc
    25501 taactaatat tgatgctaaa attaaaagtg ctcaagatga attttatcac cagttgtcac
    25561 aagctttatt aaacaacata aatgagattt ttggtataca agaaacaaaa aatatgttag
    25621 atcagtttga aaatcggtat cctgatctat taaaggaagt cttccgacat gtgactatac
    25681 agagaatttc tgaggtatta caaagattgc ttggagaaaa tatttctgtt cgcaatttaa
    25741 aacttattat ggagtctttg gcgctttggg ctcccagaga aaaagatgtc ataacattag
    25801 ttgaacatgt ccgtgcatca ctttctaggt atatttgtag taaaatagct gtttctggtg
    25861 agattaaagt tgtgatgctt tccggatata ttgaggatgc aataagaaaa gggataaggc
    25921 aaacctctgg tggctctttc ttgaatatgg atatagaggt ttcggatgag gtaatggaaa
    25981 ctttagcaca tgctttgaga gaattgagaa atgcaaaaaa aaatttcgtt cttttggtat
    26041 cagtagatat acgtaggttt gttaaaagac ttatagataa cagatttaag agtatactcg
    26101 ttatatcgta tgctgagatt gatgaagcat ataccattaa tgtattaaag actatttagt
    26161 gaggtttaaa tatgagtaac attaatttag ttcaattagt tagagatagt cttttcacga
    26221 ttggttgtcc tccatcaatt attacagatt tagacagcca ttcagcgatt accatttctt
    26281 tggatagtat gcctgctatt aatatagctc ttgtcaatga gcaggttatg ttatgggcta
    26341 attttgatgc accaagtgat gtaaaacttc agtcatctgc ttataacata ttaaatttaa
    26401 tgttaatgaa ctttagttat agcattaatg agctggtaga gcttcatcgt tctgatgagt
    26461 acttacagtt aagagttgtt attaaagatg actacgttca cgatgggatt gtttttgcag
    26521 agattctgca tgagttttat cagaggatgg aaatcttaaa tggggtctta taatcaatga
    26581 gctatacaaa attgctcact caattatctt ttcctaatag aatctcgggg ccaatcttgg
    26641 aaacaagtct tagcgatgtt tcgattggtg agatttgtaa cattcaggct ggaattgaaa
    26701 gtaatgaaat tgttgcaaga gctcaggttg taggatttca tgatgaaaaa acaatattaa
    26761 gcttgattgg aaattctcgt ggactttcac ggcaaacgtt gattaagccc actgcccagt
    26821 ttcttcatac gcaagtcggc cgtggattat tgggagcagt agtcaatcct ttaggggagg
    26881 ttactgataa gtttgctgtt acagataaca gtgaaattct ttatcgacct gtagataatg
    26941 ctcctccgct atatagtgaa agggctgcaa ttgagaagcc ttttttaaca ggtattaagg
    27001 ttattgattc tttactcacg tgtggtgaag gacagcgaat ggggattttt gcgtcagctg
    27061 gttgtggcaa aacttttctc atgaatatgc tcattgaaca tagtggtgct gatatatatg
    27121 ttattgggtt aattggtgag cgaggtcgag aggttactga aacggttgat tatttgaaaa
    27181 actctgagaa aaaaagcagg tgtgttttag tatatgcaac ttcggattac tcttcggttg
    27241 atcgttgtaa tgctgcatat atagccactg ctatagccga attttttagg actgaaggac
    27301 ataaagtagc gctttttatt gattcattaa caaggtatgc cagagcatta cgtgatgtgg
    27361 ccttagccgc tggagaatca cctgccagaa gaggctatcc ggtttcggtt tttgatagct
    27421 tacccagact tcttgaaagg ccaggaaagt taaaggcagg tggctctatt actgcatttt
    27481 acactgttct tttggaggat gatgattttg ctgatccatt agctgaagag gtaagatcca
    27541 ttttagatgg acatatatat ttgagcagaa atctagccca aaaaggacaa tttcctgcaa
    27601 ttgattcctt aaaaagtata agcagggtat ttacacaggt tgttgatgaa aaacatcgta
    27661 ttatggccgc tgcatttcgg gagctacttt ctgaaataga agagctaagg acaattattg
    27721 actttggtga atacaaaccg ggggagaatg cctctcagga taaaatctac aacaaaatat
    27781 ctgttgttga gagttttctg aagcaagatt atcgactcgg ttttacttat gagcagacaa
    27841 tggagcttat tggtgaaaca attagataag gttttaaaaa tcaaagataa atatcaacgg
    27901 tctgttaaat taattgaagc acacatatta acactaaaaa aaaaactctt tgtatcgaga
    27961 tgtggaggca ttagataaaa ggattatata cttcctgcaa ctggagaatg acttagaacc
    28021 tgtaggcgct cagagtgtta gtcagctttt caatacaaga cgtaagatcg ctatagtaaa
    28081 aaagcatatt atccaatatc agtctgaacg aattcttcta aagggaagaa ttgaagaaat
    28141 tcaaaaagat attgatgagg caaatgcttc taaaagaaaa ctgcttcata aagagagtaa
    28201 aatatgtaaa cggattggct tgattaaaag aaataacttt gccaaacagt tgatacttga
    28261 tgagttatca caagaggata tgaagtatgg cattagataa tataaaccta aattttagta
    28321 gtgacaaaca gatagaaaaa tgtgagaaac tatcttcaat agataatatt gatagtctcg
    28381 ttttgaagaa gaagaggaag gttgaaattc ctgagtactc tttaattgca tctaactatt
    28441 ttactattga taagcacttt gaacataagc atgataaagg agaaatttat agtggcatta
    28501 aaaatgcgtt cgaacttaga aacgaacgag cgacatattc tgatattcca gaatcaatgg
    28561 ccattaaaga aaatattttg ataccagatc aagatatcaa agcaagggaa aaaataaata
    28621 tcggcgatat gagggggatc ttttcatata ataagagtgg aaatgcagac aagaacttcg
    28681 aaagaagtca tacttcttct gcaaaccctg ataatctgct agaatctgat aatagaaatg
    28741 gtcaaattgg tttaaaaaat catagcttgt ctattgataa gaatattgct gacatcattt
    28801 ctttactaaa tggaagtgtt gctaaatcat ttgagctgcc tgtaatgaat aaaaatactg
    28861 cagacataac cccatccatg tcattgcaag aaaaatcaat agttgaaaat gataaaaatg
    28921 tttttcaaaa aaatagtgaa atgacttacc actttaaaca gtggggggct ggacattctg
    28981 ttagtatttc agtggagtct ggttcttttg ttctaaaacc gtcagatcaa tttgtaggaa
    29041 ataaacttga cttaattttg aaacaagatg ctgagggtaa ttacagattt gatagcagtc
    29101 aacataataa ggggaataaa aataatagta caggatataa tgaacagagt gaagaagaat
    29161 gctaagaatt aaacattttg acgctaacga aaaactacag attttatatg caaagcaact
    29221 ctgcgagcgt ttttcgattc agacattcaa aaataaattt acaggcagtg aaagtttagt
    29281 cactcttact tccgtgtgtg gggattgggt aattcgtatt gatacattat cttttttgaa
    29341 aaaaaaatac gaggtatttt caggattttc tacacaagaa tctttactgc atttatcaaa
    29401 atgtgtcttt atagagtcgt catctgtatt ttcgattcca gaactgtctg ataagattac
    29461 tttccggatc acgaatgaaa tccagtatgc aactactggg agtcatttat gctgtttttc
    29521 ctcttcttta ggtattattt attttgacaa gatgccggta ttacgtaatc aagtttctct
    29581 tgactcattg catcatcttt tagagttttg cttaggttca tctaatgtaa ggctggctac
    29641 tttaaaaaga attcgcactg gtgatataat catagttcag aaactttata atttattatt
    29701 gtgtaatcaa gttattattg gggattatat tgtgaatgat aataatgagg caaaaattaa
    29761 tctgtcagaa agtaatggtg agtcagaaca cacagaagtt tctttggcat tattcaatta
    29821 tgatgatatc aatgtaaaag tggactttat tcttttagaa aaaaatatga caatcaatga
    29881 actaaaaatg tatgtagaaa acgaattatt caagtttccc gatgacatag ttaaacatgt
    29941 aaatattaaa gtaaatggtt ctttggttgg gcatggggaa cttgtttcta ttgaggatgg
    30001 ttatggtatc gagattagtt cttggatggt aaaggagtaa tctcatgctg agtgacatgt
    30061 ccctcatcgc tacactttct ttttttacgc tattaccatt tttagttgct gctggtacct
    30121 gttatataaa gttttctatt gtttttgtaa tggtacgaaa tgcacttggt ttgcagcagg
    30181 ttccatcaaa tatgactctc aatggtattg cattgattat ggctttgttc gtgatgaaac
    30241 ccattattga agctggttat gaaaattatc taaatggccc tcaaaaattt gatacaatat
    30301 ctgatattgt ccgtttttct gattctggct tgatggaata taagcaatac ttgaaaaagc
    30361 atacagatct tgagttagca cgttttttcc aaaggtctga agaagaaaat gctgatttaa
    30421 agagtgcaga gaataatgat tattcattgt tttccctctt gcctgcatat gcgctaagtg
    30481 aaatcaaaga tgcgttcaaa ataggttttt atttatattt gccatttgtt gttgttgatc
    30541 ttgttatttc aagcatttta cttgcacttg gtatgatgat gatgagtcca atcacaatat
    30601 cagtaccaat aaaacttgta ttatttgttg cgcttgatgg gtggggtatc ctgtctaagg
    30661 ccttgattga acaatatatc aatattcctg cttagatgat ggaggtaatg tgtctgatat
    30721 agtttatatg ggtaataagg ctctttattt aatccttatc ttttccttat ggccagtagg
    30781 tatagctacg gttattggat tatctattgg tttgttacag acagtgactc aactccaaga
    30841 gcagacactt ccttttggta taaagcttat aggtgtctca atatctttgc tacttctttc
    30901 tggatggtat ggtgaggttt tattgtcttt ttgtcatgaa ataatgtttt taattaagag
    30961 tggggtttga tggacatttc aagctggttc gaaagtattc atgtgttttt aatactcctg
    31021 aacggcgttt tttttagatt ggctccatta tttttctttc ttccattttt aaataacggt
    31081 ataatttctc catctattag aatacctgtg atttttcttg ttgcatcagg attaattact
    31141 tctggtaagg tagacatagg ttcttctgtt tttgaacatg tttatttcct tatgttcaag
    31201 gaaataattg ttggcctcct tctctctttt tgcttgtctc ttcccttttg gatatttcat
    31261 gctgttggta gcattattga caaccagcgt ggggcaacgc ttagtagttc aattgatcct
    31321 gccaatggtg ttgatacgtc tgagttggca aaatttttca atcttttttc tgcagttgta
    31381 tttctataca gtggtggtat ggtctttatt ttagaatcca tacaaatgtc ttataatata
    31441 tgcccgttat tttctcaatg ttctttccgt gtctcaaata tcttaacatt tctgacttta
    31501 ttggcaagtc aggctgttat tttagccagt cctgttatga tagtattgtt actatcagaa
    31561 gtattacttg gtgtattatc gagatttgct ccgcagatga atgctttttc cgtatcatta
    31621 actattaaaa gtttacttgc aatatttatt attttcatct gttcttctac tatttacttt
    31681 tctaaagttc aatttttcct cggtgaacat aaatttttca caaatctatt tgttagataa
    31741 aatattatgg caaataaaac agaaaagccg acacctaaaa aactaaagga tgccgcaaaa
    31801 aaaggacagt catttaaatt taaggattta acgactgttg ttattattct ggtagggaca
    31861 tttactataa tatcattctt ttccttaagt gatgtaatgc tcttatacag atatgtaata
    31921 attaatgact tcgaaattaa tgagggtaaa tacttttttg cagtggttat tgtctttttt
    31981 aagataattg gcttcccact ttttttctgt gttctttcgg ctgtgttgcc aacattggtt
    32041 caaacaaagt ttgttcttgc gactaaagct atcaagattg atttttcagt attaaaccct
    32101 gttaaagggt taaaaaaaat atttagtata aagacaataa aagaattttt caaaagcatt
    32161 ctgcttctta ttattctagc attaacaacc tatttctttt ggattaatga ccgaaaaata
    32221 attttttctc aggtgttttc tagtgttgat ggcttatatc ttatttgggg gaggctgttt
    32281 aaggatataa tattattttt cttggcattt tctattcttg ttattattct tgactttgtg
    32341 attgagttca ttttatacat gaaagatatg atgatggata aacaggagat aaaaagagaa
    32401 tatatagagc aagagggaca ctttgagaca aagtcgagaa ggcgtgagtt gcatatcgag
    32461 attctttcag agcagactaa atctgatata cgtaattcaa aattagtggt aatgaacccg
    32521 actcatattg caattggtat ttattttaat ccagaaatag cgcctgcacc ttttatttct
    32581 ctcattgaaa ctaaccagtg tgccttggct gtcagaaaat atgcaaatga agttggtata
    32641 ccgactgtgc gtgatgtgaa attagctaga aaactatata aaacacatac aaaatatagt
    32701 tttgttgatt ttgaacactt ggatgaagtc ctacgtctta ttgtttggct tgagcaggtt
    32761 gaaaacactc attaataagt aaaggagatg tgttatatgg gagttaattt ctgtaataaa
    32821 ataggtattg atcagtctga atttgaaata gaatcttcca tcattaactc cattgctaat
    32881 gaggtattga acccaatatc tttccttagc aataaggata taataaatgt tttactcaga
    32941 aagatttcat ctgaatgtga cctggttaga aaagacattt atcgttgtgc tctggagttg
    33001 gtcgttgaaa aaacacctga tgatttataa tcattcataa taactctttt gctgatataa
    33061 atatttttgt tgaagatatt tatgtagtca caggatcaat tgtagaaaat tgttatgttt
    33121 ttgttaggaa ctgcactaaa taatatatga taagacagca aaaacgactg accataatcc
    33181 ttcttttatt aggagttgat aagagagact attcatcctg taatgttaaa actctgttat
    33241 atagtattcg cgattatgca aagtctgtaa atgatcatga gatactaacc gagagtaatc
    33301 gtttattatc tcattgcatt tcagatagta acggagcttt ctttaaaagt agtaaatacg
    33361 ttccccttaa gtacttacgt aaaaggagaa tagcacgaaa gataccaaat gattagcact
    33421 ttgctagtaa gcttgctggt aaactatatt gaccgttggg tttgttttga gactgtaatt
    33481 taaattgtgt aattgcctgt ttttgatatg tttactccaa caacggaga
//
LOCUS       DADLTY010000025        33056 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.025, whole genome shotgun sequence.
ACCESSION   DADLTY010000025 DADLTY010000000
VERSION     DADLTY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33056
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.025"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..517
                     /gene="mdfA"
                     /locus_tag="JN334_000996"
     CDS             <1..517
                     /gene="mdfA"
                     /locus_tag="JN334_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HAY7437226.1"
                     /translation="AQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRT
                     VRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVRLTLFA
                     SDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSLMVIFL
                     KDKQMGNSHEG"
     gene            complement(558..842)
                     /locus_tag="JN334_000997"
     CDS             complement(558..842)
                     /locus_tag="JN334_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437227.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(928..1743)
                     /gene="ybjI"
                     /locus_tag="JN334_000998"
     CDS             complement(928..1743)
                     /gene="ybjI"
                     /locus_tag="JN334_000998"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HAY7437228.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(1743..2951)
                     /locus_tag="JN334_000999"
     CDS             complement(1743..2951)
                     /locus_tag="JN334_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7437229.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            3035..3571
                     /locus_tag="JN334_001000"
     CDS             3035..3571
                     /locus_tag="JN334_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7437230.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(3746..5431)
                     /locus_tag="JN334_001001"
     CDS             complement(3746..5431)
                     /locus_tag="JN334_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HAY7437231.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            5701..6078
                     /locus_tag="JN334_001002"
     CDS             5701..6078
                     /locus_tag="JN334_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437232.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(6108..6365)
                     /gene="grxA"
                     /locus_tag="JN334_001003"
     CDS             complement(6108..6365)
                     /gene="grxA"
                     /locus_tag="JN334_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HAY7437233.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            6525..6812
                     /locus_tag="JN334_001004"
     CDS             6525..6812
                     /locus_tag="JN334_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HAY7437234.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            6796..7518
                     /gene="nfsA"
                     /locus_tag="JN334_001005"
     CDS             6796..7518
                     /gene="nfsA"
                     /locus_tag="JN334_001005"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HAY7437235.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            7579..8481
                     /gene="rimK"
                     /locus_tag="JN334_001006"
     CDS             7579..8481
                     /gene="rimK"
                     /locus_tag="JN334_001006"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HAY7437236.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            8569..9045
                     /locus_tag="JN334_001007"
     CDS             8569..9045
                     /locus_tag="JN334_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HAY7437237.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            9397..10509
                     /gene="potF"
                     /locus_tag="JN334_001008"
     CDS             9397..10509
                     /gene="potF"
                     /locus_tag="JN334_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HAY7437238.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            10604..11737
                     /gene="potG"
                     /locus_tag="JN334_001009"
     CDS             10604..11737
                     /gene="potG"
                     /locus_tag="JN334_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HAY7437239.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            11747..12700
                     /gene="potH"
                     /locus_tag="JN334_001010"
     CDS             11747..12700
                     /gene="potH"
                     /locus_tag="JN334_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HAY7437240.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            12697..13542
                     /gene="potI"
                     /locus_tag="JN334_001011"
     CDS             12697..13542
                     /gene="potI"
                     /locus_tag="JN334_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HAY7437241.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            13602..14090
                     /locus_tag="JN334_001012"
     CDS             13602..14090
                     /locus_tag="JN334_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HAY7437242.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            14131..15258
                     /gene="rlmC"
                     /locus_tag="JN334_001013"
     CDS             14131..15258
                     /gene="rlmC"
                     /locus_tag="JN334_001013"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HAY7437243.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(15433..16164)
                     /gene="artJ"
                     /locus_tag="JN334_001014"
     CDS             complement(15433..16164)
                     /gene="artJ"
                     /locus_tag="JN334_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7437244.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(16456..17124)
                     /gene="artM"
                     /locus_tag="JN334_001015"
     CDS             complement(16456..17124)
                     /gene="artM"
                     /locus_tag="JN334_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HAY7437245.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(17124..17840)
                     /gene="artQ"
                     /locus_tag="JN334_001016"
     CDS             complement(17124..17840)
                     /gene="artQ"
                     /locus_tag="JN334_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HAY7437246.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(17847..18578)
                     /gene="artJ"
                     /locus_tag="JN334_001017"
     CDS             complement(17847..18578)
                     /gene="artJ"
                     /locus_tag="JN334_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7437247.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(18596..19324)
                     /gene="artP"
                     /locus_tag="JN334_001018"
     CDS             complement(18596..19324)
                     /gene="artP"
                     /locus_tag="JN334_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HAY7437248.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(19542..20057)
                     /locus_tag="JN334_001019"
     CDS             complement(19542..20057)
                     /locus_tag="JN334_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7437249.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            20706..22475
                     /locus_tag="JN334_001020"
     CDS             20706..22475
                     /locus_tag="JN334_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY7437250.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            22686..23009
                     /locus_tag="JN334_001021"
     CDS             22686..23009
                     /locus_tag="JN334_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAY7437251.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            23006..23836
                     /locus_tag="JN334_001022"
     CDS             23006..23836
                     /locus_tag="JN334_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAY7437252.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(23833..24846)
                     /locus_tag="JN334_001023"
     CDS             complement(23833..24846)
                     /locus_tag="JN334_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY7437253.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(24945..26375)
                     /locus_tag="JN334_001024"
     CDS             complement(24945..26375)
                     /locus_tag="JN334_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7437254.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(26386..27387)
                     /gene="ltaE"
                     /locus_tag="JN334_001025"
     CDS             complement(26386..27387)
                     /gene="ltaE"
                     /locus_tag="JN334_001025"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAY7437255.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(27424..29142)
                     /gene="poxB"
                     /locus_tag="JN334_001026"
     CDS             complement(27424..29142)
                     /gene="poxB"
                     /locus_tag="JN334_001026"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HAY7437256.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(29275..30243)
                     /gene="hcr"
                     /locus_tag="JN334_001027"
     CDS             complement(29275..30243)
                     /gene="hcr"
                     /locus_tag="JN334_001027"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HAY7437257.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(30255..31907)
                     /gene="hcp"
                     /locus_tag="JN334_001028"
                     /gene_synonym="priS"
     CDS             complement(30255..31907)
                     /gene="hcp"
                     /locus_tag="JN334_001028"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAY7437258.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(32051..32950)
                     /gene="lysO"
                     /locus_tag="JN334_001029"
     CDS             complement(32051..32950)
                     /gene="lysO"
                     /locus_tag="JN334_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HAY7437259.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8235 a         8182 c         8479 g         8160 t
ORIGIN      
        1 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
       61 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
      121 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
      181 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
      241 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
      301 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
      361 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
      421 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
      481 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
      541 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
      601 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
      661 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
      721 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
      781 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
      841 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
      901 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
      961 tttatcgatc acatccagta cgccttcacg gttattgtag cctgcccggt attttgccgc
     1021 tgcgacgact gcgctgccgg cattttccat tgcaaaacta aagcctgcct gacgcagcat
     1081 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     1141 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     1201 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     1261 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     1321 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     1381 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     1441 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     1501 attaaaaaca tctttgcctt cactcattac ccagccgccg ttttcagcca caaaggcaat
     1561 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     1621 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     1681 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatgct
     1741 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcgac ggctttcgcg
     1801 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     1861 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     1921 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     1981 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     2041 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     2101 accacggcaa cgcgactgta actgtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     2161 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     2221 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     2281 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     2341 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     2401 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     2461 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     2521 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     2581 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     2641 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     2701 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     2761 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     2821 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     2881 ggcaaaaaga agaacataaa cagcgcccag gttcggcgtt tcaatgcatt acgtgaagaa
     2941 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     3001 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     3061 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     3121 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     3181 ttcaggaatt gatgagttgt tgctggaggc gttcagcagt tttactgaga ttatgtcccg
     3241 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     3301 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     3361 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     3421 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     3481 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     3541 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     3601 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     3661 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     3721 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     3781 tccctgccag cgtcagcagg acgttggcga ttgcgtaggt gcccgcatag cccagcgcag
     3841 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     3901 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     3961 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     4021 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     4081 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     4141 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     4201 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     4261 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     4321 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     4381 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     4441 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     4501 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     4561 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     4621 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     4681 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     4741 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     4801 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     4861 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     4921 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     4981 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     5041 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     5101 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     5161 ttttcccatc gcgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     5221 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     5281 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     5341 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     5401 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     5461 tgttgaaaga aatgctaatt tacgataatg tgttttacca gaattcaggg cgcagattca
     5521 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     5581 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     5641 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     5701 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     5761 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     5821 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     5881 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     5941 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     6001 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     6061 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     6121 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     6181 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     6241 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     6301 tcagccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     6361 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     6421 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     6481 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     6541 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     6601 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     6661 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     6721 aggtggcaaa acgtcttgcc ccacagctga tgaactgtcc accgcaatat tcacgttcag
     6781 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc
     6841 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     6901 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     6961 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     7021 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     7081 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     7141 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     7201 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     7261 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
     7321 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
     7381 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
     7441 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
     7501 ggttgggcga cgcgctaaaa ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt
     7561 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
     7621 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
     7681 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta tttactacaa aggccgcaag
     7741 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
     7801 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
     7861 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
     7921 cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
     7981 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
     8041 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
     8101 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
     8161 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
     8221 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
     8281 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
     8341 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
     8401 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
     8461 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
     8521 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
     8581 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg acctggggat gagttttttt
     8641 gaatgtgata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
     8701 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
     8761 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
     8821 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
     8881 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
     8941 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
     9001 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
     9061 acgcagtcgc tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
     9121 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
     9181 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
     9241 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
     9301 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
     9361 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
     9421 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
     9481 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
     9541 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
     9601 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
     9661 ctagaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
     9721 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
     9781 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
     9841 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
     9901 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
     9961 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    10021 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    10081 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    10141 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    10201 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgccagcgga tgccaaaaac
    10261 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    10321 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    10381 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    10441 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    10501 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    10561 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    10621 tcgcccgcag gcgaaaaccc gtaaggcgct gacgccgcta ttagaaatcc gcaacctcac
    10681 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    10741 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    10801 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    10861 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    10921 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    10981 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    11041 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    11101 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    11161 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    11221 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    11281 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    11341 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    11401 tggcttggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    11461 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    11521 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    11581 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gtgcccagct
    11641 acaaaacgcc catcgtcatc gtaaagggtt accgacctgg ggcgacgaag tgcgtttgtg
    11701 ctgggaagtg gacagctgtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    11761 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    11821 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    11881 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    11941 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    12001 ttttctgcaa ctgaccgacg atccgctcta tttcgatgct tatctccagt cgctacaggt
    12061 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    12121 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    12181 ctcgtttctg atccgcgttt atgcttggat gggaatatta aaaaacaacg gtgtgctgaa
    12241 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    12301 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    12361 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    12421 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    12481 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    12541 ggacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    12601 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    12661 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    12721 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    12781 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggg cggctggtca
    12841 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    12901 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    12961 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    13021 ccgctagtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    13081 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    13141 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    13201 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    13261 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    13321 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    13381 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    13441 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    13501 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    13561 acctggctac ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    13621 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    13681 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    13741 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    13801 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    13861 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    13921 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    13981 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    14041 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    14101 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    14161 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    14221 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    14281 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    14341 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    14401 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    14461 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    14521 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    14581 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    14641 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    14701 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    14761 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    14821 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    14881 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    14941 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    15001 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    15061 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    15121 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    15181 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    15241 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    15301 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    15361 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    15421 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    15481 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    15541 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    15601 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    15661 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    15721 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    15781 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggaaa tcacgacggc
    15841 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    15901 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    15961 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    16021 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    16081 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    16141 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    16201 ccgtgtctgc ggcatgggat aacaatgcca tcaagtgaat ttatatgcaa taaacatgat
    16261 taaataattt aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta
    16321 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    16381 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
    16441 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    16501 catcatcagc gtcagcaggc cgttgacgac caagtaaata atccctgccg caccgaacac
    16561 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    16621 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    16681 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    16741 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    16801 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    16861 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaactggc ccggcccgta
    16921 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    16981 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    17041 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    17101 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    17161 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    17221 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    17281 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    17341 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    17401 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    17461 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    17521 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    17581 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    17641 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    17701 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    17761 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    17821 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    17881 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    17941 tttccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    18001 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    18061 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    18121 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    18181 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    18241 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    18301 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    18361 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    18421 gcctgcgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    18481 ggaggatagg gggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    18541 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    18601 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    18661 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    18721 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    18781 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    18841 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    18901 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    18961 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    19021 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    19081 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    19141 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    19201 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    19261 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    19321 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttaa
    19381 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    19441 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    19501 aatgccggat aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    19561 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    19621 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    19681 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    19741 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    19801 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    19861 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    19921 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    19981 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    20041 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    20101 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    20161 ctgattgggt ttgagtccat tgctggcgac ccactcaagg ttgctcgtag ctgacttact
    20221 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    20281 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    20341 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    20401 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaatatg
    20461 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    20521 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    20581 tgaagaataa attaaattgc ttcagtattt cgttaattaa tataaataca aaaggcgttg
    20641 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tgggtgattt
    20701 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    20761 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat cccagtcgta
    20821 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    20881 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    20941 aatttaatac attttttagc aatactttcg agattgatgt taaaaaatat ctgggtaaat
    21001 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    21061 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    21121 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    21181 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    21241 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    21301 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    21361 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    21421 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    21481 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    21541 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    21601 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    21661 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    21721 gtaatttagt caatcttaaa acactactca atgatggttg tttcattaaa tatagtcatt
    21781 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    21841 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    21901 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    21961 ttaataacgt aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata
    22021 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    22081 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    22141 gtttactatc tctgtactct atctgtgcaa gggatatata taatatgaac gccgggaata
    22201 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    22261 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    22321 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    22381 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    22441 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac
    22501 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata atcgtaaaga
    22561 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    22621 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    22681 cgatcatgca attttctaca accccaactc tggaaggcct gaccatcgtt gaatattgcg
    22741 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    22801 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    22861 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    22921 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    22981 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    23041 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    23101 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    23161 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    23221 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    23281 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    23341 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    23401 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    23461 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    23521 gcgcaaagac gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg
    23581 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc
    23641 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    23701 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    23761 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    23821 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    23881 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    23941 atccagcgta aagtcaaaat tcaacttgga ggcgccgtag tgggtcagcg gcggctcttt
    24001 tgctgacttg cggcctaaac gctccatgct gcgggcgatc atatccagca tcgggtaagg
    24061 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    24121 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    24181 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    24241 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    24301 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    24361 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    24421 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat tgcgatggtg
    24481 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    24541 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    24601 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    24661 cactttagct tgtgatgaaa ccagctcggt cagatccgtc ggaacaaact ctgcgcccat
    24721 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    24781 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    24841 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    24901 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    24961 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    25021 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    25081 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    25141 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    25201 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    25261 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    25321 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    25381 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    25441 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    25501 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    25561 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    25621 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    25681 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    25741 acctctggtc cggcggcttc gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc
    25801 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    25861 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    25921 cctgcgccaa cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga
    25981 atgtccgccg tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc
    26041 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    26101 agagccagct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    26161 aaatagacgg tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg
    26221 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    26281 gccgctagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    26341 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    26401 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    26461 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
    26521 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    26581 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    26641 caaccgtgcg acgttatttt ccagggcata catcccggcg gcagccagaa tcccggactg
    26701 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    26761 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
    26821 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    26881 gcgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    26941 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    27001 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    27061 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    27121 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    27181 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    27241 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    27301 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
    27361 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    27421 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    27481 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
    27541 aatggctaat tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
    27601 ggcgcgttgc agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc
    27661 gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta
    27721 acctccggcc ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat
    27781 tttcaccggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
    27841 gccatcgccg cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc
    27901 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    27961 gttcattttc agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat
    28021 ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    28081 ctcgctcggt ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc
    28141 tttatccaga aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt
    28201 tgatttgata tcgccgacca gcgccatatc taccttgcta tgcgcgccga tgctggctgg
    28261 attaatatca atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg
    28321 agtaccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc
    28381 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg
    28441 tagggcatga acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
    28501 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
    28561 cagtttgcgt aactcttctt cttccggcat cacgactggc tgtggcgcat gataccagtg
    28621 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cccggtaata caacaacgga
    28681 aacgccacgg ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc
    28741 cgggctggaa accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
    28801 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
    28861 cggaacgtta ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc
    28921 gcacgatccg gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa
    28981 cgccgccact tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact
    29041 gtcgctaaga ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga
    29101 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
    29161 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
    29221 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
    29281 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
    29341 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
    29401 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
    29461 cgcctccagt agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
    29521 gaatttcaga ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa
    29581 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
    29641 gcaggtcatc acggtacgtg aagctaagtc aggtacatct gccagcagtt cgcgagtgag
    29701 gcgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
    29761 acgccactca tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac
    29821 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
    29881 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
    29941 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
    30001 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
    30061 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
    30121 ttgcccggcg cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg
    30181 cgtttcttgc gtaatgtgat gaacctgtat ccgccacggg cattgattcg ttggcatcgt
    30241 catcgacaaa ctccttacgc gttcaacagt tgcttcatgt cttcttcaac agtggtgata
    30301 gaacgcaggc cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt
    30361 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca
    30421 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag
    30481 cccagtttct ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga
    30541 cctgcatcta ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga
    30601 tatttaccac aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg
    30661 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
    30721 agtttttcac ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga
    30781 ccaaagccca cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc
    30841 gcctgggcga tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca
    30901 acgatgctgc gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag
    30961 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
    31021 ccgctgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
    31081 ggcagcattt cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg
    31141 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
    31201 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
    31261 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
    31321 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
    31381 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
    31441 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg
    31501 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca
    31561 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc
    31621 tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg
    31681 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
    31741 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
    31801 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
    31861 ttttctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
    31921 aaagttatat ttaatataca tgtttaaggg taagacgctt aacgcgggga taaaagggat
    31981 ttttcatgca actttaaggg agattgattt agcgcaattt tggcggcagg gaactaccgc
    32041 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
    32101 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
    32161 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
    32221 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
    32281 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
    32341 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
    32401 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
    32461 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
    32521 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
    32581 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
    32641 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct
    32701 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
    32761 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
    32821 taaaaaagag aataaggtaa accatccagc ttaatagctg attaatagct tttaacgcag
    32881 cttgttggcg aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc
    32941 cagaaaacat gaaaatccag tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta
    33001 ctgaaagcgg aagggcgagt aaagttgcaa ttaaaaggaa atgttatgca tcagag
//
LOCUS       DADLTY010000026        32077 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.026, whole genome shotgun sequence.
ACCESSION   DADLTY010000026 DADLTY010000000
VERSION     DADLTY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.026"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..1150)
                     /gene="setB"
                     /locus_tag="JN334_001030"
     CDS             complement(<1..1150)
                     /gene="setB"
                     /locus_tag="JN334_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HAY7437260.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIAT"
     gene            1518..2648
                     /locus_tag="JN334_001031"
     CDS             1518..2648
                     /locus_tag="JN334_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HAY7437261.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2648..3586
                     /gene="fruK"
                     /locus_tag="JN334_001032"
     CDS             2648..3586
                     /gene="fruK"
                     /locus_tag="JN334_001032"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAY7437262.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3603..5294
                     /gene="fruA"
                     /locus_tag="JN334_001033"
     CDS             3603..5294
                     /gene="fruA"
                     /locus_tag="JN334_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HAY7437263.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5718..6659
                     /locus_tag="JN334_001034"
     CDS             5718..6659
                     /locus_tag="JN334_001034"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HAY7437264.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6647..7585
                     /locus_tag="JN334_001035"
     CDS             6647..7585
                     /locus_tag="JN334_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HAY7437265.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7679..8929
                     /locus_tag="JN334_001036"
     CDS             7679..8929
                     /locus_tag="JN334_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY7437266.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHNVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="JN334_001037"
                     /pseudo
     CDS             complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="JN334_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9825..10766
                     /gene="rihB"
                     /locus_tag="JN334_001038"
     CDS             9825..10766
                     /gene="rihB"
                     /locus_tag="JN334_001038"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HAY7437267.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10866..12116
                     /locus_tag="JN334_001039"
     CDS             10866..12116
                     /locus_tag="JN334_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY7437268.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12172..13260)
                     /locus_tag="JN334_001040"
     CDS             complement(12172..13260)
                     /locus_tag="JN334_001040"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7437269.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="JN334_001041"
     CDS             complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="JN334_001041"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAY7437270.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14194..15243)
                     /locus_tag="JN334_001042"
     CDS             complement(14194..15243)
                     /locus_tag="JN334_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HAY7437271.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15342..16223
                     /locus_tag="JN334_001043"
     CDS             15342..16223
                     /locus_tag="JN334_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7437272.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16429..17898
                     /gene="lysP"
                     /locus_tag="JN334_001044"
     CDS             16429..17898
                     /gene="lysP"
                     /locus_tag="JN334_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HAY7437273.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18190..20181
                     /gene="cirA"
                     /locus_tag="JN334_001045"
                     /pseudo
     CDS             18190..20181
                     /gene="cirA"
                     /locus_tag="JN334_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="JN334_001046"
                     /pseudo
     CDS             complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="JN334_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21306..21974
                     /gene="folE"
                     /locus_tag="JN334_001047"
     CDS             21306..21974
                     /gene="folE"
                     /locus_tag="JN334_001047"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAY7437274.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21991..23067
                     /locus_tag="JN334_001048"
     CDS             21991..23067
                     /locus_tag="JN334_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HAY7437275.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23209..24249
                     /gene="galS"
                     /locus_tag="JN334_001049"
     CDS             23209..24249
                     /gene="galS"
                     /locus_tag="JN334_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HAY7437276.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24529..25527
                     /gene="mglB"
                     /locus_tag="JN334_001050"
     CDS             24529..25527
                     /gene="mglB"
                     /locus_tag="JN334_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HAY7437277.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25588..27108
                     /gene="mglA"
                     /locus_tag="JN334_001051"
     CDS             25588..27108
                     /gene="mglA"
                     /locus_tag="JN334_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HAY7437278.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27124..28134
                     /gene="mglC"
                     /locus_tag="JN334_001052"
     CDS             27124..28134
                     /gene="mglC"
                     /locus_tag="JN334_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HAY7437279.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28170..29383)
                     /gene="preA"
                     /locus_tag="JN334_001053"
                     /pseudo
     CDS             complement(28170..29383)
                     /gene="preA"
                     /locus_tag="JN334_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29377..30615)
                     /locus_tag="JN334_001054"
     CDS             complement(29377..30615)
                     /locus_tag="JN334_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY7437280.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30809..31048)
                     /locus_tag="JN334_001055"
     CDS             complement(30809..31048)
                     /locus_tag="JN334_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HAY7437281.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="JN334_001056"
     CDS             complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="JN334_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAY7437282.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31920..>32077)
                     /locus_tag="JN334_001057"
     CDS             complement(31920..>32077)
                     /locus_tag="JN334_001057"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Reclaims exogenous and endogenous cytidine and
                     2'-deoxycytidine molecules for UMP synthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAY7437283.1"
                     /translation="LILLNLKGYDYPDIQRAVLAEKADAPLIQWDATSATLKALGCHS
                     IDRVLLA"
BASE COUNT         7844 a         7793 c         8464 g         7976 t
ORIGIN      
        1 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
       61 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga
      121 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
      181 cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg
      241 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
      301 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca
      361 gcccggcggc ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga
      421 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
      481 tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
      541 ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa
      601 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat
      661 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
      721 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
      781 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
      841 tgcgattcca agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
      901 gcgatttgcg atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc
      961 caatgacagc gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat
     1021 cggtaaggaa aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg
     1081 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg
     1141 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     1201 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     1261 gctgattggc tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca
     1321 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     1381 tttccttgcg tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg
     1441 tctttaaccc taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc
     1501 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     1561 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     1621 tagcagaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     1681 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     1741 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg
     1801 tggcaatcgg tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc
     1861 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     1921 ttcgcgcatt actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     1981 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     2041 tgaaagaggc gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     2101 tgaatctcgg acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga
     2161 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     2221 tgagtgtggc gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt
     2281 tgctcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     2341 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg
     2401 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     2461 gtgatattac cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga
     2521 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     2581 cagatgctga acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg
     2641 gcgcataatg agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg
     2701 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     2761 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     2821 tggcggcttc ctgggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     2881 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     2941 aaaagacggc gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg
     3001 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     3061 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     3121 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     3181 agcggcaccg tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa
     3241 actgcctgaa atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc
     3301 gcatgttgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     3361 gatcgctaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     3421 tggtggcctg atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc
     3481 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     3541 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     3601 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggcga
     3661 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     3721 acgcagaaat ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta
     3781 aaaatgtctg gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     3841 aagccaaagg ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg
     3901 ccagcggtcc gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct
     3961 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     4021 aaacccgtgg ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg
     4081 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     4141 tgtatcgtac ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg
     4201 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta
     4261 agaaagagag tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga
     4321 tggtcgttgc tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     4381 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     4441 tgatggtgcc ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca
     4501 cgccgggtct gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta
     4561 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     4621 cgcagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     4681 tcggtctggc gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga
     4741 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     4801 gcatgatgtg tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg
     4861 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     4921 caccgctggc aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     4981 aggaaggtgg caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc
     5041 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga
     5101 ctggcgcaat ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg
     5161 ttctgctgat ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg
     5221 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     5281 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     5341 atgagcgcaa agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat
     5401 ctttcagtag aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt
     5461 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt
     5521 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     5581 tggcggtaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     5641 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt
     5701 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     5761 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     5821 taaaatttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta
     5881 acaaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     5941 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     6001 cgagttattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     6061 atattagcaa cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg
     6121 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
     6181 atgccgccaa cgtacccgta tttgtcgatc cggtttcagc gtggaaatgt gtcaaagtgc
     6241 gcgaccgtct aaatcagaac cacactctga agccaaaccg ccttgaagcg gaaaccctga
     6301 gtggaattgc gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac
     6361 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca
     6421 gcggtgaaaa tggctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg
     6481 gcgatgccat gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat
     6541 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
     6601 ccgatttatc gattgccaac gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa
     6661 aatttcccct gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa
     6721 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
     6781 ccagaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat
     6841 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg
     6901 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
     6961 cggaaaaaat ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat
     7021 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
     7081 tatttctgcc gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc
     7141 gaaatctatt ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt
     7201 aattggctat cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt
     7261 cagcattcgt ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag
     7321 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga
     7381 ggaatctatc aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat
     7441 tggtaaagaa agtacaccgt tcctgctggc gcgcgttgct gagctgacca gcggtgacag
     7501 cctgaaatcc aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa
     7561 agaataccag cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat
     7621 caagcatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat
     7681 ggatataatg agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc
     7741 agtgaataaa aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc
     7801 tattggtggc atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt
     7861 aggcgttcat aacgtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct
     7921 ggttgggccg aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt
     7981 acttccggcg attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat
     8041 ggggctgctg attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat
     8101 cgaatctttt gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt
     8161 taaaccgttt atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat
     8221 ggcgtccatt gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta
     8281 cctgttagcg gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag
     8341 cccggcaacc gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc
     8401 aaaaagcttt atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg
     8461 tgtagcgacg gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg
     8521 cggaattggc ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt
     8581 gctggcaccg ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgctggtag
     8641 cctgattggg cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata
     8701 cctgcaaacg ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg
     8761 tggttttgct aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc
     8821 aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc
     8881 caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg
     8941 tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt
     9001 tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac
     9061 tgctttctgt ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt
     9121 gcgcgagaac atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat
     9181 gcttttcgtg ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca
     9241 ctaacggaaa tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta
     9301 aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt
     9361 tcatagggag cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt
     9421 ctttgtcgat taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg
     9481 acacgcgacc attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca
     9541 gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc
     9601 gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt
     9661 gctgcttaag gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc
     9721 atatttagca tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt
     9781 gctgtagaaa aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa
     9841 ttattctgga ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac
     9901 atccggcaat agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa
     9961 cattaattaa tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg
    10021 ggatgccgca acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg
    10081 gactggatgg cccggtattc gagccgctga cccgccaggc agaaagtacc catgcggtga
    10141 aatatatcat cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc
    10201 cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg
    10261 aaattgtgct aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca
    10321 atatctttgc cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga
    10381 tgatgggcct cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg
    10441 aaagggtagg cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa
    10501 cgcagttcga atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt
    10561 atctgatcaa ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca
    10621 gcggcccttg ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta
    10681 ataccaaagt cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg
    10741 tgcgcggcta catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat
    10801 gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa
    10861 tcactatgga tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt
    10921 tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac
    10981 aggtcgtgat tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag
    11041 tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg
    11101 gttctctggt cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt
    11161 tcagggtatt accggcaatt atcttcgtca ctgcactggt gagtattctc tactacatcg
    11221 gtgtgatggg gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca
    11281 gcaagatcga gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg
    11341 cgatcgtcaa accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta
    11401 gtggcatggc ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg
    11461 tggaatattt gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc
    11521 tgttaagccc ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa
    11581 caccgccaaa aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg
    11641 ccgccggtgt agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta
    11701 ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag
    11761 gttacctgct ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg
    11821 ccgggagttt gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct
    11881 caccctatct gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg
    11941 cgttgtgcgg tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg
    12001 ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt acgcgcgctg gcggcggcga
    12061 cactttctaa tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg
    12121 caatttatga gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat
    12181 tggcactaat gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc
    12241 gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc
    12301 cagaaagcta tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt
    12361 cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga
    12421 ctcatcgggc aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc
    12481 ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa
    12541 agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc
    12601 tgaaacggta tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc
    12661 ctcggctgtc aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga
    12721 cccgttcagt agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac
    12781 ggtttgatcg tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg
    12841 aacgcagccg gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata
    12901 aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg
    12961 ggcgatattg cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc
    13021 ttgcgggtaa tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac
    13081 gcagtattcc tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa
    13141 atccataata tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt
    13201 ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttattcat
    13261 cttcaggcta ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag
    13321 atatccgggt tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata
    13381 atccagcgga acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg
    13441 cggctgccaa aggtactttt cgcatcgtta aggtgcatcc cgcgcaaata cttaaagccg
    13501 acaatacggg caaaatcagc gaacgttttt tcgcattcgg ctggagtacg caaatcatac
    13561 ccggcagcga aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg
    13621 ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg
    13681 gtgttttcaa tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg
    13741 gcaatacgcg caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag
    13801 ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc
    13861 gatttttcca gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga
    13921 agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt
    13981 tgcgtcgtga gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg
    14041 gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg
    14101 tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat
    14161 gatagcgggt taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata
    14221 gttaatcgca ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg
    14281 tttcgcccca gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc
    14341 cattgccagc aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag
    14401 gtggaacgag ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat
    14461 tttgcttttc tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat
    14521 gaggaacgga gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc
    14581 atccgggctg atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc
    14641 aatgtagata ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg
    14701 gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt
    14761 actggcttcc gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact
    14821 gctaccggca ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag
    14881 gaagcaagcc agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat
    14941 accgacatcg gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg
    15001 taataaatat tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata
    15061 gatggtgttg cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc
    15121 acccgcaacc gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag
    15181 ggctaacccc ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt
    15241 cataaccctc tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg
    15301 attatatatt tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc
    15361 agttggaagt ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc
    15421 tggcgttgtc gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg
    15481 ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct
    15541 atccgcgagc gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag
    15601 acaacggcgc gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag
    15661 ttatcgcccg ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca
    15721 gccaggacgt gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac
    15781 cgtgccacag cactgaaatc atttctgaac cgtggctgga agacgagctg gtggttttcg
    15841 ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc
    15901 cgtggatcct gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt
    15961 cacatttacc gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg
    16021 cggtgcgtca tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc
    16081 aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc
    16141 gtatacatca tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg
    16201 atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt
    16261 cgtgacgcag cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa
    16321 tccctgggcg atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat
    16381 cgtctgctac aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa
    16441 actaaaacta cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg
    16501 attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct
    16561 caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc
    16621 ctgatgacca gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact
    16681 tacggtcaga actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac
    16741 aactgggcgg tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg
    16801 ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg
    16861 aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc
    16921 acgacagtta ttgtctttat catcgttggt gtgctgatga ttatcggtat cttcaaaggc
    16981 gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt
    17041 gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc
    17101 ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag
    17161 gtattctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt
    17221 ccgtataccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc
    17281 accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt
    17341 ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac
    17401 accctggcgt gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg
    17461 ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg
    17521 tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc
    17581 gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga
    17641 cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca
    17701 ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc
    17761 attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg
    17821 ttcggctaca agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg
    17881 cagaacgata agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct
    17941 ttttcaattc catttccctg acaaatcatg cggatataaa atttaacatt tggattgata
    18001 attgttatcg tttgcattat cgttacgacg caatcaaaaa aggctgacaa atcagaggct
    18061 gttccggctt tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac
    18121 gcaaggggac gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg
    18181 agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt
    18241 tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca
    18301 tcttccgtgg aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa
    18361 gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta
    18421 caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc
    18481 tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac
    18541 aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt
    18601 ggcccgatgt cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc
    18661 aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat
    18721 cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt
    18781 gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac
    18841 tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc
    18901 aatgtcgaat ttgcctagac accgaatcaa aatcacgatt ttactgccgg atacggtttc
    18961 gaccgtcagg atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac
    19021 tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag
    19081 aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc
    19141 gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa
    19201 tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg
    19261 tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg
    19321 ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt
    19381 gcctacatgg tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca
    19441 tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc
    19501 gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg
    19561 gggctttact acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt
    19621 ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca
    19681 ccgggctacc aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta
    19741 tttagctact acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt
    19801 ccgttcaacg atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc
    19861 agcaacggcg aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg
    19921 gactggaaac cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg
    19981 cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc
    20041 ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc
    20101 gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg
    20161 gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca
    20221 gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac
    20281 tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt
    20341 ctgccaatac cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga
    20401 taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt
    20461 ccagcatgca tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac
    20521 gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt
    20581 cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc
    20641 gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat
    20701 ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc
    20761 taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac
    20821 cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc
    20881 gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg
    20941 gggggggaga aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca
    21001 gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt
    21061 gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc
    21121 acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca
    21181 ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaagctgt
    21241 aaatcagctt aataactttg cccccacgca gggtggaggc gtcacacctg caggagaaat
    21301 cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgagag
    21361 gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc
    21421 ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca
    21481 gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc
    21541 tggattacgc caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa
    21601 tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt gttaccatcg
    21661 atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg tcaaaaatta
    21721 accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa
    21781 ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg
    21841 tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct
    21901 ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc
    21961 gccatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtcacgct cgattttgtt
    22021 cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag
    22081 gcggcttatc tcaatcccgc ctggtacgat gctattacgc cgcaggatgc atggacctgg
    22141 gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct gctgtttggt
    22201 gctggcctgc aaatgttgct gccccgtggc agacgctgga tccagtcgcg gttaacgctg
    22261 ttagtcttgt tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct
    22321 tacgggctgg tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc
    22381 agccgcgcct ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac
    22441 ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg
    22501 ggcgcacagt atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc
    22561 agcggctggc tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg
    22621 gtggcgattg gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg
    22681 gcgtatcgct ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag
    22741 gcgattggct atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg
    22801 gtgcttgcga tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg
    22861 atttgtacca cgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg
    22921 ctggcgtttg ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt
    22981 tacttccgcc aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga
    23041 ccggcaatat ctaaaacatc aagataacga tctggattac attcgttaac aaaacggctg
    23101 taaccgtttc cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata
    23161 gccgccagca agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt
    23221 cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac
    23281 agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat
    23341 tatcggccaa acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg
    23401 gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc
    23461 gcccagcagc atcagaaata cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag
    23521 cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa
    23581 gcattgagtg acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc
    23641 aaccgcgttg tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc
    23701 cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc
    23761 agccacggca ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag
    23821 caggatatta ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt
    23881 gaggcggcga tggttgaact gctggggcgc aatctacaac ttaccgctgt atttgcttat
    23941 aacgacaata tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg
    24001 ttacatctct caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa
    24061 ttaacgaccg tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg
    24121 cagggggcag caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg
    24181 cgtcgccatt ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag
    24241 gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta
    24301 tgattgcacc gttttaatgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag
    24361 gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa
    24421 cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccccgcc
    24481 gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag
    24541 gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct
    24601 gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc
    24661 aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct
    24721 cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag
    24781 gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt
    24841 gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc
    24901 tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat
    24961 ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt
    25021 cagttcgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac
    25081 gtgattaaag aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca
    25141 atgtgggaca ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc
    25201 aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca
    25261 ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgcgct gccagaagcg
    25321 ctggcgctgg tgaaatccgg tgcactggcg ggcacagtac tgaacgatgc taacaaccag
    25381 gcgaaagcga cctttgatct ggcgaaaaac ctggccgatg gcaaaggtgc ggctgatggc
    25441 accaactgga aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt agataaagac
    25501 aacttggctg aattcagcaa gaaataagac tgattgggcg cagtcgttta ctgcgccctt
    25561 attgtatggc caacaaggta aaattatatg gtcagctcaa cgactccgtc ttccggggaa
    25621 tacttgttgg aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac
    25681 gttaatttaa aagtccggcc acattctatc catgcattaa tgggggaaaa cggtgcagga
    25741 aaatcgacat tattaaaatg cctgtttggt atttatcaaa aagactccgg caccatttta
    25801 ttccagggta aagagatcga tttccattcc gccaaagagg cactggaaaa tggtatttcg
    25861 atggtacacc aggagttaaa cctggtatta caacgttcgg tgatggataa catgtggctg
    25921 gggcgatatc ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa
    25981 gcgatttttg atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc
    26041 gtttcgcaaa tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg
    26101 attatggatg aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt
    26161 attcgtaaat taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaagaa
    26221 atcttccagt tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa
    26281 ccgctggcag gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaat
    26341 cagcgttttc ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta
    26401 acgtcactgc gccagccgtc gattcgcgat gtctcgtttg atctgcataa aggggagatc
    26461 ctcggtattg ccgggctggt gggggcgaaa cgtaccgata ttgttgagac gttatttggt
    26521 attcgcgaga aatcggctgg caccattacg ttgcacggca aacagatcaa taaccataat
    26581 gccaacgaag ccataaacca cggatttgca ctggtaactg aggagcgccg ctcaacggga
    26641 atttatgcct atctggatat tggttttaac tcgttaattt ccaatattcg caactacaaa
    26701 aataaagttg gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat
    26761 tcgatgcggg taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat
    26821 cagcaaaagg tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat
    26881 gaaccgacgc gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa
    26941 ctggcgaaga aaggcaaggg gattattatt atctcctctg aaatgcctga gttgttaggg
    27001 ataacagacc gtattctggt catgagcaat ggtctcgttt ccggaattgt cgatacaaaa
    27061 acaacaacgc aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt
    27121 cccatgagtg cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac
    27181 gtcgttcttt tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg
    27241 ttgaacttaa gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg
    27301 gcagggttaa ttgtcaccca ggggaccgat ctttctgctg gtcgtcaggt agggctggcg
    27361 gcagtggtgg ctgcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa
    27421 atggcgacga tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc
    27481 ggtttgatca acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc
    27541 ggcacgatga tcatcgtcta tggcatcaac tcgctctatt acgactttgt cggggcgtcg
    27601 ccaatttctg gttttgacag tggcttctct acctttgctc agggctttgt cgcgctgggg
    27661 agtttccgtc tctcttacat caccttctac gcgttgattg cggtggcgtt cgtctgggtg
    27721 ttgtggaaca aaacccgctt cggtaagaac atttttgcca ttggcggtaa cccggaagcg
    27781 gcaaaaatat ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc gttgtctggc
    27841 gtgttctatg cctttggcgg gatgttagaa gccggacgta tcggctctgc caccaacaac
    27901 ctcggcttta tgtatgagct ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc
    27961 agcggcggtg tggggacggt gattggcgtg gtgaccgggg taattatttt taccgtcatc
    28021 aactatggcc tgacgtatat cggcgtaaac ccatactggc agtacatcat caaaggggcg
    28081 attattatct tcgccgtagc gctggattca ctgaaatacg cgcgtaagaa gtgatgtgat
    28141 tgcggatatt gccggatgcg tctctggttt tacaacgtta ccgggtgttc tttctcgcct
    28201 ttcttaaact tcacttcccc gagctcaata caacccaccg ggcagacgtg accacaaagc
    28261 agacaaccca cacatttctc ggtattacaa tgcggtgtgc gggttttctc gctccattcc
    28321 atcgcctggt gaccgccgtc gtaacaggaa atataacagc gtccacagcc aacacatttg
    28381 tcaagattga tacggggata gacaatataa ctgcggtcta aatcttccgc cgggacaata
    28441 ttgttattcg ccagacctac catctcctgc aacgaatcaa atccctgatc ggcgagataa
    28501 tgcgataacc cgctcgccat atcttctacg atgcgatagc cgtactgcat aatgccggtg
    28561 gtgacctgta acgttgctgc gccgagcaat aaaaactccg ccgcatcttc ccaggtttca
    28621 atgccgccga taccgctgat tgggaaatcg cgcagttctg gatgggtgcg catttgctga
    28681 atgaagcgca gggcgatcgg ttttaccgct ttaccggaat atccggaaat actcgattta
    28741 ccgttaacga tcggcatacc gattttctga ttaagatcga tattggtgat ggatttaacg
    28801 gtgttaattg ccgcaatgcc atctgcgccg ccgcgcttcg ccgccagcgc cacttcgcac
    28861 atatcaccga tattcggcgt catcttcgcc agcattggca gcgtggaacc ccgtttcact
    28921 gcccgacaat atttttctac cagctccggg ctttgcccga catcgctacc catcgcatga
    28981 gaagtcattt gcggacagga gaagttacac tcgatcatat ccgcgccagc tgctcccatt
    29041 gctgctcatt ttcccccatg atcgaagcga tcaatacttt gtccgggtaa tcttccttca
    29101 gccgacgcag ggcggtcaga ttctcttcca acggatgttc agcaatctgc tccatatttt
    29161 tgaagccgat aaaaccggta tcttctttca ccagatgatc aaaacgcggc gagacttcgt
    29221 tggcgataaa aaagccgatc gttttaaaca ccacaccgcc ccaacctgtg tcgtaggctt
    29281 tggcacacat ctcatagcag ttgcctaccg gcgaagaaga gaggcagaac gggttgggaa
    29341 agttcacgcc gcaaaaagta atcgaaagat ctttcgttaa catgagcaag ctccctctaa
    29401 atagtgatga atcgcctcgg cggcttcttt cccggttttc acggcataga ccacggtttt
    29461 gtcaccctca acaatatcgc cagcagcaaa gacttgcgga tcgcggatct gataattttg
    29521 tgttttgatg gtgttacgct gctgctctaa ctccgcaaag gcatccagtc tggcatgctg
    29581 accgacggcg agaataattt tatctgccgc catcgtcagt tcgcctgata accgtacatg
    29641 cttaaacgtg actttattgc cttccacggc tactggcgtg aatccatcga tgatcgaaac
    29701 gcccagctcc cgggcgctgg caaactcttt ttcgcttgcc ggaaactcat ctaactcttc
    29761 acgtgctacg caagttaccg cctgacagcc gagaactttc agcgtgctgg ctacgtccat
    29821 cgcgacatca ccgccgccga taattaatgc gctttgtgga atgctgatat cgccttgcgc
    29881 ctgtcgtaca cgttgcagga agtcgacggc aatctcaacg tcgctatgct caaacagcgg
    29941 tagcccggaa ccgcttgata accccacggt gaccagtacc gcgcggtttt ccgctttcag
    30001 ttgctccagg gtgagtgttt taccgatttc gttattgcac ttaatggtca cgcccatttt
    30061 ttcaatacgg gcgatctctg catccagcac tgattgcggt aaccggaatt gcggaatacc
    30121 gttacgcaac cagccaccgg ggtgcgcttc tttctcataa atcgtgacgt cataaccctg
    30181 gtttgtcagt gtcacactgg cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt
    30241 gccgagcgtt ttagtaccgg gctgataaat ttccatcccg gtttgttgtt caaaatcagt
    30301 aacaaaacgc tgtaagcggc cgatatcaat gggagcatca acaccggcac gggtacaacc
    30361 gctttggcat aatttctccg tcgggcacac tctggcacaa acggcaccga gggcattatt
    30421 ttcgcgaatt gtctcggcag cgcctttaaa attacgaaag tagattgagc gaataaattt
    30481 ccccggatcg gtctgtgccg ggcaagcctg actacaggga gcgtcgtgac ataataaaca
    30541 gcgagaggct tctttaatcg ccagtaacgg cgtaaaagcc ggagtgagtt catccagata
    30601 attttgttgc ggcatgtgat cttccttaat gctatgagta attatggcga acgctaaata
    30661 aatattgcgt gctgatatta aggctatgcg cgtggggaaa tttaagctgt gaaaggattc
    30721 acaaatagct gcggttcaga atgttatatc cgcagcttat tgagtgaagt aataattatt
    30781 ctcaataatt aacagaaaag agaaaatatt aacgatagaa aataagataa acaatcatgc
    30841 cgatagataa aattccgaag ccaatatatg ccaccatata agcgaagaag agtccgggat
    30901 tttttgctcg ccagacatac accggttccg gtgcattttt aacccgttta tcaatcgcgc
    30961 ctgcgcgcca gcctttccat gcttcgcgga aacaaaatat cgggataagg aaaaaaacgg
    31021 caacgacaaa aaactgctgt acatccataa ctactttcct tgtttctttt gtaattcaag
    31081 taactgttcg ggtgtaacgg cgggataccc ctgcgcatct tccggcacct ggtgcatagc
    31141 cggaataggg accagcggcc ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc
    31201 cagcgcaccg aaacgggcgg caaattcgcg aatacgtact gacagcatat ctttcggtga
    31261 cggtacggca taacactgag cctgaatccc catatgcagc gcaataaata atgctcgctc
    31321 acagtggaaa cgttgggtga taataatgaa atcattagta tcgaaaactt tgcgtgtacg
    31381 cacgatggaa tccagcgtac gaaagcctgc gtaatcgaga acaatatctg atgggtcgac
    31441 accagcagcg attaaatctt tgcgcatggt catcggctcg ttataacttt gcaatgcgtt
    31501 atcgccgctc agtaatagat aatttacctt accgctgtta taggcattaa tcgctccttg
    31561 aatgcggtag cgataatact gattaataac gccggtacga taatattttg ctgtgccgag
    31621 caccacgccg acctggcggt aggggagatc ctgcaattcg tcgtagatat aaggcgcggt
    31681 tttccagctc atccagcgat cgaggccgag cacagtcaac agcagcaagc cgatcaggac
    31741 taacaggctg aggaacacgc gctttaacat gcaggagact cgagctaagg aaaatttcca
    31801 tcaggctact ttacccgctg gttttgcgca agaaacggca aactgtttgc cgggaatttc
    31861 gacaccagga gagggctggc aagccggaca aggcgttcac gccgcatccg gcaccaggct
    31921 taagcgagaa gcacgcggtc gatactgtga cagccgagag ctttcagcgt tgcggaggtg
    31981 gcatcccact gaatcaacgg cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc
    32041 gggtaatcat aacccttgag attcaacaga attaacg
//
LOCUS       DADLTY010000027        31678 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.027, whole genome shotgun sequence.
ACCESSION   DADLTY010000027 DADLTY010000000
VERSION     DADLTY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.027"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..239)
                     /locus_tag="JN334_001058"
     CDS             complement(<1..239)
                     /locus_tag="JN334_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HAY7437284.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRP"
     gene            complement(252..911)
                     /gene="eutL"
                     /locus_tag="JN334_001059"
     CDS             complement(252..911)
                     /gene="eutL"
                     /locus_tag="JN334_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAY7437285.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            2093..2884
                     /locus_tag="JN334_001060"
     CDS             2093..2884
                     /locus_tag="JN334_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437286.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            3604..3999
                     /locus_tag="JN334_001061"
     CDS             3604..3999
                     /locus_tag="JN334_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7437287.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYLKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            4017..4280
                     /locus_tag="JN334_001062"
     CDS             4017..4280
                     /locus_tag="JN334_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437288.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            4303..4845
                     /locus_tag="JN334_001063"
     CDS             4303..4845
                     /locus_tag="JN334_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437289.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            4942..5271
                     /locus_tag="JN334_001064"
     CDS             4942..5271
                     /locus_tag="JN334_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437290.1"
                     /translation="MFGNYISTSPEKIIMLALRIMQGIAKTLAEHVLDLKHSPLSKQA
                     MKRQTLRLWAEYSLGTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGID
                     INDGTGD"
     gene            5310..5516
                     /locus_tag="JN334_001065"
     CDS             5310..5516
                     /locus_tag="JN334_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437291.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            5975..6313
                     /locus_tag="JN334_001066"
     CDS             5975..6313
                     /locus_tag="JN334_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7437292.1"
                     /translation="MLDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMA
                     EPEGRCVRMLSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEP
                     DLRSCANMLA"
     gene            6294..6572
                     /locus_tag="JN334_001067"
     CDS             6294..6572
                     /locus_tag="JN334_001067"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437293.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            6565..8025
                     /locus_tag="JN334_001068"
     CDS             6565..8025
                     /locus_tag="JN334_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7437294.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            8047..8868
                     /gene="murI"
                     /locus_tag="JN334_001069"
     CDS             8047..8868
                     /gene="murI"
                     /locus_tag="JN334_001069"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY7437295.1"
                     /translation="MRLNMFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNM
                     NAPYGNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPI
                     LSIIEPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLI
                     EEGNLNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAH
                     DQAISIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(8987..9681)
                     /locus_tag="JN334_001070"
     CDS             complement(join(8987..9435,9435..9681))
                     /locus_tag="JN334_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY7437296.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(10200..11573)
                     /gene="eutA"
                     /locus_tag="JN334_001071"
     CDS             complement(10200..11573)
                     /gene="eutA"
                     /locus_tag="JN334_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HAY7437297.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(11548..12027)
                     /gene="eutP"
                     /locus_tag="JN334_001072"
     CDS             complement(11548..12027)
                     /gene="eutP"
                     /locus_tag="JN334_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HAY7437298.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(12040..12375)
                     /gene="eutS"
                     /locus_tag="JN334_001073"
     CDS             complement(12040..12375)
                     /gene="eutS"
                     /locus_tag="JN334_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HAY7437299.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(12668..14947)
                     /gene="maeB"
                     /locus_tag="JN334_001074"
     CDS             complement(12668..14947)
                     /gene="maeB"
                     /locus_tag="JN334_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HAY7437300.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            15236..16186
                     /gene="tal"
                     /locus_tag="JN334_001075"
     CDS             15236..16186
                     /gene="tal"
                     /locus_tag="JN334_001075"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY7437301.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            16206..18209
                     /gene="tkt"
                     /locus_tag="JN334_001076"
     CDS             16206..18209
                     /gene="tkt"
                     /locus_tag="JN334_001076"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY7437302.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(18312..19355)
                     /locus_tag="JN334_001077"
     CDS             complement(18312..19355)
                     /locus_tag="JN334_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HAY7437303.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(19481..20056)
                     /gene="nudK"
                     /locus_tag="JN334_001078"
     CDS             complement(19481..20056)
                     /gene="nudK"
                     /locus_tag="JN334_001078"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HAY7437304.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(20124..22103)
                     /gene="aegA"
                     /locus_tag="JN334_001079"
     CDS             complement(20124..22103)
                     /gene="aegA"
                     /locus_tag="JN334_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HAY7437305.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            22309..24026
                     /gene="narQ"
                     /locus_tag="JN334_001080"
                     /pseudo
     CDS             22309..24026
                     /gene="narQ"
                     /locus_tag="JN334_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            24228..27341
                     /gene="acrD"
                     /locus_tag="JN334_001081"
     CDS             24228..27341
                     /gene="acrD"
                     /locus_tag="JN334_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HAY7437306.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(27440..27499)
                     /gene="ypfM"
                     /locus_tag="JN334_001082"
     CDS             complement(27440..27499)
                     /gene="ypfM"
                     /locus_tag="JN334_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HAY7437307.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            27880..28236
                     /locus_tag="JN334_001083"
     CDS             27880..28236
                     /locus_tag="JN334_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HAY7437308.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            28240..29367
                     /gene="dapE"
                     /locus_tag="JN334_001084"
     CDS             28240..29367
                     /gene="dapE"
                     /locus_tag="JN334_001084"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAY7437309.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            29395..29595
                     /locus_tag="JN334_001085"
     CDS             29395..29595
                     /locus_tag="JN334_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HAY7437310.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(29705..30403)
                     /gene="ypfH"
                     /locus_tag="JN334_001086"
     CDS             complement(29705..30403)
                     /gene="ypfH"
                     /locus_tag="JN334_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7437311.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(30477..>31678)
                     /locus_tag="JN334_001087"
     CDS             complement(30477..>31678)
                     /locus_tag="JN334_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY7437312.1"
                     /translation="HQLVSRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQ
                     PIRWAQGCPLEKMVSEALVFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLS
                     GAHYRTSPLDLRRMMDAPGQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRP
                     RGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYL
                     SVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRL
                     RRDAQALAQWNGEMLPVDPLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSE
                     LALPALRGRLQKNASDAQLCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRI
                     TQWQFFH"
BASE COUNT         8084 a         7927 c         7798 g         7869 t
ORIGIN      
        1 tctggccgac cgatctcctt acggctgatg acacgcccgg tacgcatcgc ggcagcacaa
       61 cctgcgtcca gcgccgcacg acacgccgcc agatcgcctt ccaccaccag cgttaagcga
      121 ccagggtcaa gcacttcgtg actgagcaga cgaacgttag ctgctttgag catggcatcc
      181 gctgcatcta tcgcggcgac catgccgtcc acttccagca atcccagtgc attgatcatc
      241 ggcaacctcc gttacgcacg ctggattgga ttacgcgcga tttccagcac tgcatcggta
      301 aaggcgttac aggctgcttt acacgcggcc tggctaccgg ttaaaaatgc tgccgagtag
      361 ttggtttcag acggtggcgg gacgtaggtc accagctgta cgtcggcaga tttcaacgcg
      421 gcatcaatgc cgtaggtcgc ttccagcggc ggtgccacca gatacgccat cggatcgcca
      481 agcgtgatcc cggcggttga tgaaagataa gaaccggtac gcgaaaccac atgcgccagg
      541 aaggcggtgt tttctgcgtc attggcccac tggaaagctg cgccattttc aatatgtgca
      601 accatcgcat ccagaccggc acgcacttcc gccgggtttg gcccaccaag cataatcagc
      661 acttcaccgg cagtcggtga cgggccgtgt gccgctcccg catacagcga gcgaccatat
      721 accacttcga ccatcgcttg tttggtcgct tcgtcagccg caatgtaggt tacgtcgtca
      781 gaatcggcag aaatcagccc gaggctacga atatgcggcg gcaattttag ttcacgcgca
      841 aattcagcgt taacggaggc aatcacccgc atggcggtta ccgacggtcg aatcaaatct
      901 aaagctggca tgatgcctcc ttatcgggtc atgttgatgc cggatgcttt ctgctccagc
      961 atacgtttcg ccaaatccac aatgacggca cagcagcacg aaagagaagc aaaagtcagc
     1021 tacctggtac actaggcagc gggtcagcaa aggggggcaa atgccccttt tttcatgtca
     1081 gataatcaga tcattacctc aataaactaa taatcacatc gcggttacaa ccagtttgat
     1141 agtgatagct caaacccaga cgtggcgctg gctgcaagca aaataagccg caaaaatgcg
     1201 accccatgtt tactagccgc aaaaatgcgg tcctatttgc cagaaccttt tattattcaa
     1261 ttggttagtg caagaaaaag cgtgattact gttcaagaag tatccagaaa ggtcatgatt
     1321 ttgacgctga aaaaaaatag gtgggcattt ttgcccactc actagccgca aatttgccca
     1381 ctcactagcc gcatttttgc ggcccaaagt aataaataca gagaaataat aaatacagat
     1441 cagcgccaag atttttcgtc ttttcggcgg aatcatgaga ctagtgctta cgtcctgata
     1501 acctccagag cagaccttaa cttctgtgca gcaggactac gctcattgcc gctcggctag
     1561 gtcaaatcct cacgataaag ctgttcggat tgtgaccata atgttaaagg actctttaaa
     1621 tcattccttg acgaattaac tagctggctt aagcggaata gcatgcagga taaaaacaaa
     1681 accagcttag cagtggggag agcttcgctt cgctcgtctc ctggggccaa tgctcaatga
     1741 aacgacgtaa ggcacggagc gctcaccata ggtagacaca taaacatctg agagcgcacc
     1801 ttctatcgcg ctacgcgctc aataaaaacc aacgacaccg ccacactgca agaccaatgg
     1861 tttttatcgc gtatacagac cagacaggaa acagcagcaa agcagcgtat agccaggtgc
     1921 agacagcaca ctcacacgac gcacagcagg cttaagtgat cactcaggaa actttatccc
     1981 cgccgccgat caccgcgaac tatcatgacc gcgccaaatt cgaaaggaat ggcccataga
     2041 acgcgatgaa cacacataag cgctacactt acttaacgtc gccacagagc gattgagagc
     2101 gcttaaaatc gatgcgattt cattaaattt ttctcactcg cctgaatggt ggcgatcagg
     2161 actgatcgca acattgcccc gcctcgatat tgagcgcata atagatacat acattaagcg
     2221 gaaaagagat aagtttattg tgaaaatcag ggggttacat atcatgcgca tatcagaaca
     2281 acaacggcag atcctcgact atctggcaaa aaacgaatca atcaccgcac gacaagccgc
     2341 agatctcgtt tatggtggca atgttacccg cgtccaggtt gacgccgccc gccgctcact
     2401 catgacgatg gttagtaatg ggctaatcag gaaacaaggc cgcgctttcg tagctgaaag
     2461 acatatcaat cacgcttata gccaggacgt tgaagaattg cgccgccaga tcctttgggt
     2521 attcagcaaa cagggtcagg agtatttcct ttctgaacac ccaaatttcg ccgccaaaga
     2581 gttaacagta agcggattgt cgtgcgcact ggattatctt gtcggtgaag gttgcgttac
     2641 ttacaagcag ctatggcacg ccctgaatac gcttgtagaa gatggtttac tcattgccac
     2701 gcgtgagaac atcgaatact ggataatgcc gcgttcaggt cgccgcaatc atgcacggta
     2761 tatttcaggc aacagataca gaacgccgga ctttaatccg acacaggaag atcgggaccg
     2821 tgccgaaatg atgtgcaggg aacaggagaa aagaattatg gataagttat tttcgatgca
     2881 gtgaacacaa gcgggcgcaa tgcccgtttt tattgcctta ttccacgcct gacgaatcaa
     2941 tttgatcaca cctgagaaaa tgcatagcca gaagttaagg ccgttatatt gcgattggga
     3001 gcgttttaaa cggtatcaat ttaattaatt ctgatgtggt gatttgcagg caaaaaaaac
     3061 ccgccggaga agcgggaaat agagacatat cgttgtagtt attaacagat cactttgagg
     3121 tcgagggcgg ctaaccctcg ctaatattat cccgctatta ccttgcgggt gcaaataatc
     3181 ttttattgct ttgatgattt aataaatagc actttttgct aacaaaaacc gctgtcaata
     3241 tatggccaaa aatgtcaaca gaaaaaataa ggtttataat attaagtatc acatgggtaa
     3301 tgggttcagg gaatcggaat ccaccgcctc acggaaattc aaaatttgct tcttctttcc
     3361 tccgtgggcg gcttcttttt cgtatcgagg tatttatgaa agcaataaca ttagaacagt
     3421 tcgcagcaat ggcgaatgta tcgaccagaa cgctgaggcg ctggctaaaa aaaccatcaa
     3481 ccggagtccc tttgccattt accccacgcg gtactcgcca attattctgg gagcacgaat
     3541 gcaaagaatg gttgtacaga acagaacgat aataatcacg catcacattt tgaggtaaac
     3601 atcatgacca atatcgaaag attagaacgc tgtgccgata ttatgcgacg cagatggatt
     3661 tatgaccctg aagcgggttt attgtttagc cgtgaaacaa aagacgttat caaaggcagc
     3721 atgaccaata aagggcattt ggttgtaacg gtacatgaag gtaaattcaa agtgacactc
     3781 acctatctga aagcctgcta tgtatacaca tacggcgctt atgacgagac attatatgaa
     3841 gtgcaccacg ttaatttgaa caaacaggat aaccgcatca agaatattcg cctgatgccg
     3901 aaagaaaagc accgccggat acataaaaac gtccgcaaac ttattcggct gggccacaca
     3961 caatggcttc aaccgcaatt aattgaacaa tcactataaa cacaggagta aataccatgc
     4021 attcattaga cagttatttt cagcgcacga cagcccctaa atcagccgcc caggaacgcc
     4081 gtgaggaatt ccatgagaaa gtgatgcgtt ctgctgatta tattgcggac aagttcgtgg
     4141 aaacggttcg tccacttgtt gatgaagtag ctgataaact tcagtctgaa atgccggaag
     4201 atatggaagg cactgcaaag cggcgactca tttgcgaatt aagtcgtcgt tttggcgtgt
     4261 caataagcgc ttttaaataa tcgaaaaaag gggggaaaga taatggacgc atttgaggta
     4321 ctggcaaata aaggcgctga actggtagca cagcgtgata aaaccgccaa cgaaggggaa
     4381 cgcgcactgc taaataaaca gattaaagcg atccgtatgg cccagtttaa attaatcagt
     4441 aatgaagtta tcgaaacaat taaaccgcag atcccacaaa tcgttgctga cgctattaaa
     4501 gccgccggac tggagaaacg cattgccggg ctaaaaacga acgcagagaa aatggagatg
     4561 tacaaacaga gcggtgctcc gaatcccgat gagtacttct caccggatgt tgagtttatg
     4621 gcgcaggttg aggaacgtct gggtagctgt ctcaccgagg aacagcgtcg ctattttgat
     4681 ggagtggata gttcggcagg tattgacctg aactcgtatt tcggtcgtga aattgagcat
     4741 tttgatgccg ccccgtctat gccggagcca gaaccagaaa tggccaccga tcctgaagct
     4801 gagcaacgga aacgcgtatt caatgcgata gggattcggt actgatcgga taaaagaaag
     4861 ccgggaatta ccccggctga cttaacaatg tgaccaatga acaaatcgga caagacaaac
     4921 aggataagca gtctcgaaac aatgttcggg aactatatta gcacaagtcc ggagaaaatc
     4981 atcatgttgg cactacgcat catgcagggc atcgctaaga ccctcgcaga acatgtctta
     5041 gacctgaaac attcgccact gagcaaacag gccatgaagc gccaaacgct tcgtctgtgg
     5101 gctgaatatt cgcttggaac tatcaataaa atcatcgaca tgaaatcggg gccgagcaac
     5161 cagagcgcgg aggaaatgga gttcatcagg cgtttaattc tcattcgcag agacattcat
     5221 agccagttac acagcgtggg aatcgatatt aatgatggga cgggcgatta attttcggac
     5281 aagtactatg cgcgcgcgag gagctgaaca tgaagcaaga tattgcagac aggctggaga
     5341 tactcgaagg ccagcgggca gaagcgaaac aactacgtaa gcaggccaga agagcgcaca
     5401 ggaacaacga agcagagtta ctaaccaaat acatcagttt caccaattac tgcatctacg
     5461 agtgttacaa ggaagatgca gaggactggc tggattcatt gccagaacag tactgagcag
     5521 aataacacca cgtcatgttg ttctgaggtg agcatatcac ctctgttcag gtggccaaat
     5581 ttagtgtgcc acttcagcat caccgttgaa atcacaataa cgattttttt gtaaatatgc
     5641 tctcacatat gtaggaatag accaggttgg cattgaaaca catctaccat gaccataata
     5701 aagtaatttt ttgatatgtt tatggtttta tattaattaa aatatatcta aatttgatag
     5761 ttttaaaatt gcaaaatcca gattgtgttt gtaatgttta atctgattgt attgagtggc
     5821 gtttattatg aaaaataaat caggtaattc ttgttataga gaagcagtcg atattcatag
     5881 ctcacttgag ctttcattta tattgcataa atatcatttg cgtgtagatg agaagaaatc
     5941 tcgaaatgta ttcagcgcca tacgtgatga gctcatcctt gatggggatt cacgacaaaa
     6001 cttagctact ttctgtcaaa catggcttga tgatgaaatc cgtgagttgc ttaatttagc
     6061 tattgataaa aacatgattt ataaagatga atatccacaa atggcggaac ctgaaggtcg
     6121 ctgtgtgcgt atgctttccg attcgtggag tttccccgat tcgcgacaca ccttaggttg
     6181 ctcaaccatt ggttcatctg aagctgctat gctcggccgc ttggcgttaa aatggcagtg
     6241 gtgtaaaaaa cgcgaagtac aaggtaaatc aaccgaacct gatttgcgat cctgtgcaaa
     6301 tatgctggca taaattctgc cgttactgtg atgtgaaatt gtgtgaaatt ccacttgatg
     6361 atcatcacct gataatgaat gctaaagagg ttttgaagca tgtcgatgaa aacactattg
     6421 gtgtcgttac gactttaggt attacttatt tttgtaaagc agattgcgtt agtgttttta
     6481 agataagtgt taaagttaat attagttttt atatttacat cgtaattata tgtttctatt
     6541 ttaaaaatgt tcgagagtat aaaaatgtct aatgctaata tcgcacccaa aagtaagaaa
     6601 atgacagtgg ggacactcgc tatcatgaat ataactgctg tagtaagtct acgatttata
     6661 ccttctgaag cagaatacgg cttaggtgcc attttttact atacctttgc tgcaattatg
     6721 tttttagtac ctgttgcctt tgttgcggct gagctagcta caacatatcc acaaaaaggg
     6781 ggggcatttc gatggatagg agaagcattt ggggctcgct ggggttttgt aggaatgctt
     6841 atgacctggc tacaagtcat cccctacttt ccaacagtac tgacatttgg tgcagtctcg
     6901 gttgcattta ttgatgttga tatgggggtg gcagaatcaa ttgccagtaa taaatggtat
     6961 attttatttt tcgtactatt tgtttactgg ggagctattt gtgttgcatt acgtggcgtt
     7021 ggtattttct cgcaggtatc taagtggtgc ggtattatag ggacaattat tccagctatt
     7081 atccttgtta ttcttggttt ctcctatctg tggttcagcg gtaaaccagc ggtgatccca
     7141 ttaggttggg gtgatctttt ccctaatata gctaacttcc agaatattgt tcttgccgcc
     7201 agcatatttc ttgcttatgc agggatggaa atgaatgcgg tccatgttaa cgatttagat
     7261 aatccaacta aaaaataccc aattgcaatt accatcgctt ctataggtac tattgcaata
     7321 ttcctgctca gtactttagg ggtcgccttt attattccta cagataaaat tagtctaact
     7381 caaagtttgt tggttgccta cgatatgctg ttcgaatggg ctggagtgcc atggcttggc
     7441 tctgtgatgg catttatgct agcaattggc gtactgggtg gggtggttac ttggattgca
     7501 gggcctaata caggtgtatt agcgatcgcg aaagctggtt atttacctaa gtttttccaa
     7561 aaaaccaatc gtcatggaat ggggcaccat ctaatgtttg tacaaggtat aattgtctca
     7621 gttctttctg ttacttttgt gataatgcct tcagttcaag cagcttttca gattttatca
     7681 cagttaacag ttatgctcta tttagttatg tatatgctca tgttcgctag tgctatctat
     7741 ctacgacatt ctcaacctga tacaaaacgc ccatatcgag cccctgcttt atttttctgg
     7801 gcaacacttg gattcttagg gtcttttctg gcttttgtat taagttttat tccgcccgag
     7861 caaatcccaa ctggtagtcc aaccagctat gttctttatt tgttagtatt agttgcccta
     7921 tttttattaa ttccaatatt aatttacgcc ttccgaaaat ctgactggaa agatccaaac
     7981 tctgatttcg aaccattttc ttgggaaaaa agaactagta attaaatttt ccataagcta
     8041 caaaagatga ggttaaatat gtttagtgct gaatctccaa ttggtgtaat agactcaggt
     8101 gtcggtggat tgacatcggt aaaggaaata attaatttgc ttcctggtga agatattatc
     8161 tattgtggag ataatatgaa tgcaccttat ggcaaccgtt ctgccgatga tatcatcagt
     8221 ttgactaaaa aaatgctgac ttttttacag tcaaggaatg tcaaactagt tgctgtcgca
     8281 tgtaatacta tatcctcaac tttggaatcc gaagaataca gcggatatgc taagagcttt
     8341 ccgtttccta tcttatccat cattgaacct gcagtagaag atgtgattcg ccagcaatat
     8401 aaaaatgttg gtattatcgc taccgagttt actattaaaa cggggtgtca caaagagcta
     8461 ataaaaaaat tgaattcaac aataaatgta tttggagaac caagcaaaaa tcttgcaatg
     8521 ttaattgagg aaggtaatct taatgctcct gcaatcttga atgacatcaa aaaacatgtt
     8581 aaccatttaa tttccctcca tccagtaaat gaaattattc tcggctgtac tcactatcca
     8641 attgttcaga atttttttga agcagttgca ccggatataa aatttattaa tcctgcacat
     8701 gatcaggcta tctctattaa aaatcacttg ggacagctaa atttattaaa ctcctcaaat
     8761 ataggaactt tgcaaataaa tactagtgga agtatggaga tctacaaaac ggtactaggg
     8821 gaattgagca ttacgaaacc tcatacattc agtatcaggc agttttaaat ttatccggta
     8881 caccgtaaaa tcccgtcctt ctgaactgca ccccaaaagt tggacaccaa ctgagtaagg
     8941 tgcattttat ggacaaaccc agttacatac tgggtagtgc atccaattag tagaacatgt
     9001 gtttttcgat aaacgctccg atcacttttt cgtggatctc aactgagcgt gagaagcaga
     9061 ttgttttacg acccaaccgc ttaatgcggg tgcgtagcgt cagattatta cgctcaatgc
     9121 gttgggtgaa tattttgccg gtcagatgct tattcttcgg cacctcccgg ccatagctgc
     9181 cccagtcatc gctggtcagc atgccgatgt tgaagggtgt aagcagtgcc agtagctccc
     9241 ggcacgtttg atcggttcgg ggaccaaaag tgtaggccag tacaccgcct gttttggtgt
     9301 tgtacgcgta cccgagccaa tgttgccggg ctttactgcc aacgtagctc cattgctcat
     9361 caagctcgca gataaacgcc acatcagcat gggcaacagg cgaagaagtt attcgctttg
     9421 gcgcgagttt tttaaagtcc ggatgacggt gttactacca attttcagtg tcctggcggt
     9481 atcgcgaacc ccggcaccat taaaggccat ttcagttatc agctctttca tacccggctt
     9541 acgtgcttga taagtataag tgaactgaaa cacacggtgg cagtcacggc agcgaagtct
     9601 gtcacggcct ttagggttct gaccatggcg gtaaacctga gctgactgac aacggggaca
     9661 atgaatatta acgctggtca tgagataacc tcaaaagccc gtattataca tcagattcaa
     9721 ctaattagag gcatcaccca tccagcatta taaaacatac taacaataac taaattataa
     9781 taatcacaac ttacaatcaa ataataaatg cataagatta acaatgattt tttcgggtta
     9841 tatttatcta aaaaagcaga atattatgta acttattatt ttctaagcga aaatttattg
     9901 tgcggtcaga ctaacaaact tgcgtaccac ccgaagaaca tgcgaagatt taagtattct
     9961 ttcttttttg gtttttattt ttcattttct cttttcttct ctttccggtt ctgttctggt
    10021 tttggttttt tcctttttat ttttattctt tcttcagggt tatgctaaaa caccaaaaca
    10081 tttttcacca caaaagcatc caatcatctt gccagaccaa tcaccttaac cggatacact
    10141 gccggatata atcccactaa cacccagaca ccaagcacca tacctgataa ctaccgtatt
    10201 caggaaggaa atgcgagtga tttcaccgtc accggcacaa ccgatccgcc aaaaagaggc
    10261 gtaccaatgt cgatatagtc ccccgcgcgg acaatcactt cgtcaatgac tgccaacggg
    10321 agttgttgta gctgtgggcg caacaacatg cccagagctt taccaaagtc ctgcccggcc
    10381 accaccagca ggggatgcgg attcggaaaa cgcgcgacga aatcgaccag cgcgttgatg
    10441 accgtcagaa ccgcggcgta acgcacaggc agcgaggcgg gaagcgccag cacgtacacg
    10501 tcagttttgg gatcaagatc cagctgaagc agcgcctgtt gccaggcact caccagatcc
    10561 gtttcatcaa tcgggatcgc caccggcaaa ttgcgcagcg gcagttgtac gccctccagc
    10621 cagattgtgc tgccagagag cgaaagggta tgtgcacccg cgccaatcac cgtggagcgt
    10681 acggtttgcg ccggaaactg cacattcatc tcacgcaggc gcggatggtc atgcagcgcc
    10741 gttgccagaa gtgggccaat atcggcaaaa cagaacgggt tggcgggctg gtggcgataa
    10801 cattcgccca cgcctccaga aagcgtaatg atttcgggcg taacacctgc gggcagcaaa
    10861 ccggtttgca tcaatgcctg cgcgagcggc gaaagcgttc cgtcaatcac ttcgacaatc
    10921 agcgccgcca tccgccgggt aacctgcacc agctgcgcgc cggtcagcga acgggcgtca
    10981 gtgcctgcac cgaagcactc atccacaatc atctgccccg gtttatgagc gtaaaccacg
    11041 cgcccctggc tgtcggtttc cagcaggcga ccaccgacgt tgagacaggc agtgccgctg
    11101 atttttccgg catcgaacag ggcgtagttc gcggtgccac cgccgatatc gatattcagt
    11161 acccgacaca gccgttgttc agaaagggtt tgcgccccgg ctccgtgacc ggcgatcacg
    11221 gattcgaggt gcggcccggc gctggcgacg acaaaatcgc ccagcgactg tttttccttc
    11281 atcacttttt ccatcagctg tgcgaccagg ctttcggtaa actggccttc cggcagctgg
    11341 gcaatgatgg tttcgcggat gcgctggctt tcggttttgt cggctggcac gctggcgggc
    11401 acggacgccg ttaccgggat cgattcatca cattcggtga tggtaaaacc cagtagatcc
    11461 gccacttcac gggcttccgg ggtaataatg ctggcgcgca gaaccacgga cattgcctgt
    11521 tcgccgcgcg cgtgtgcttc acgaatatca ttcgctgtga tgagtttttt cacctgcttc
    11581 ctcctgtttg gtgagagatg ccagataatc caccagttgc tgcacgcttt gcgggtcgtg
    11641 gctgtttagc tcgaaaatcg gctcttcgaa acccgtctca agcaataact ttcgcgttgc
    11701 ggcgacgtca gcatctggca tgtccgtttt actgatgacg gcgatttgcc gtttgctgac
    11761 gccaatatcc agcaacccgg caggcaaccg actttccggg tcatttgcgc catggacata
    11821 gatcagcatg tctacatcct gtagcgtggt aattaaggcg tgataccagc ggggatggct
    11881 aaaatattca cccggcgtat caatatcgcc cttatcatta aattccacgg ccagtgtttt
    11941 tctggcgagg gtataattcc cctgcaacgc attaaataac gttgtttttc ctgccccgac
    12001 gctacccaca aacgcaattc gtttcatgaa tgcctcgtat taacttttgg tcatttcaca
    12061 aagcgtataa tttaataagc gacctaaacc gctgacggtt tgtgataacg cttcttctac
    12121 tgcgcccacc gagccataaa tcaccaatgc accgctgaag cggtcgagaa aaccgatatg
    12181 cacatcagcg gctttcagcg ctaaatcacc ggcgatcatc gccgtttcgc cgggagttag
    12241 cgtcataatg ccaattgcgc ccgcatccgg aacgccgatt ttcttcgcca gttcctcacc
    12301 gggatgcgcg atgagatgcg ccagtgtgac ctgtttgccg ggcacaaatt cctgaatgat
    12361 gcgttcttta tccatgagtc gccgcctgtg aaaaactttc gtgtatcgtg acaatttttc
    12421 gtcagagtaa ataacaaaat tcggcaagcg cgtttaaaag gtgaggtaga tcaataagaa
    12481 tggagagata tttactgtta tgaataaact ttcatatgcg acgcatgaat ataaataaaa
    12541 taatatcgag atattttata tactgatttt tttattattc gcaaactcag taataagctt
    12601 tgctaatata tttcaggaaa accgcttacc ctgaatattc agggtaagcg tgagagttaa
    12661 gaatgaatta cagcggttgg gtttgcgctt caaccacggc cagcgccacc atattgacga
    12721 tacgacgcac cgatgcgatc ggcgttaaca cgtgaaccgg tttcgccacg cccatcagca
    12781 ccggaccgac agtcacacct tccgagctgg aaacgcgcag taagttgtaa ctaatgcggg
    12841 cagcttccat gttcggcatc accagaatat tggcagaacc tttcaaaggg ctgtccggca
    12901 tacggtcgtt gcgaatcgct tccaccagcg ctgcatcgcc gtgcatttca ccatcaatca
    12961 tcagatctgg cgcacgatcc ctgaccagtt ccagcgcctg acgcattttg ctcgacgacg
    13021 ggcagtcaga agaaccaaag ttggagtgcg acaacagagc aacgcgcggc tcaataccaa
    13081 aacgacggac agtttctgcc gccatcaagg tgatctccgc cagctcttct gcgtctggtt
    13141 cgtcattgac gtaggtatcg gcaataaagg tgttaccact cggcagcagc agcgcattca
    13201 ttgcacctgc ggtgtgaacg ccatcgcgat aaccaaagac atttttcacc acactaaaat
    13261 gttcatgata atcgcccacc gtaccgcaga tcattgcatc ggcttcccca cgctgaacca
    13321 tgatcgcgcc gatcactgtc gggttactga tcagcgcacg ctgcgcctgt tcctgagtga
    13381 caccgcgacg cttcatgatc tggaagtatt cggtccagta ctctttaaag cgcggatcgg
    13441 attcgttatt gacgatttca aaatcaacgc ccgctttgat ctgcaagccc agtttctgaa
    13501 tgcgcatttc gatcacgttc ggacgaccga taaggatcgg tttcgccagt cccagcgtta
    13561 ccagttcctg agtcgcatgc agaacgcgcg cctcttcccc ttccggcaga actacgcgct
    13621 tcggcgcttt gcgagcctgg gagaaaatcg gcttcataaa caggttggtt ttgtaaacga
    13681 actcagtcag cttgtcgatg tagacgtcga aatcagcaat cggacgcgtc gccacgcccg
    13741 actccatcgc cgcttttgcg accgcaggag cgatcttaac gatcaagcgc ggatcaaacg
    13801 gttttggaat gatgtattcc ggaccaaagc tcagatcctg aacgccatac gctgaagcca
    13861 ccacttcact ctgttctgca tgggccagtt ctgcaatcgc gcgtaccgcc gccagtttca
    13921 tctcttcgtt gatggcggtt gcgccaacgt ccagcgcgcc acggaagatg aacgggaagc
    13981 aaagaacgtt gttcacctgg ttcgggtagt cggaacggcc ggtacagatg atggcatccg
    14041 gacgcacttc tttcgccagc ggcggcagaa tttccggttc cgggttcgcc agcgccagga
    14101 tcattggtgc ccgagccatt ttcttcacca tttcctgggt cagcactttc gggccggaac
    14161 agcccaggaa aatatccgca ccttcaatca catcatcgag ggtacgtttg ccgtcatcca
    14221 ccaccgcata tgcggctttg gtttccgcca tatttggctc acggccctga tagataacgc
    14281 cttttgaatc gcaaaccacg atgttatgtt tttgcagccc cagcgctacc agcaggttca
    14341 tacaggcgat tgctgcggca cccgcgccgg aaaccaccat ccgcacgtcg gagatgtttt
    14401 tctccaccac gcgcaagccg ttgaggatgg cggcagtgct gatgattgcc gtgccgtgct
    14461 gatcgtcgtg gaataccgga atattcatcc gctcgcgcag tttctgttca atatagaaac
    14521 attctggcgc tttaatgtct tcgaggttga tgccgccgaa ggtcggttcg agcgcggcga
    14581 cgacttcaat aaatttgtcc gggtcgagtt catcaacttc aatgtcaaat acatcaatcc
    14641 cagcgaattt cttaaataga acgcccttgc cttccatcac cggtttgcct gccagcgcgc
    14701 caatgttgcc taaccccagc accgccgtac cgttagagat caccgcaacc aggttaccgc
    14761 gcgcggtata tttgtaggct tttagcgggt ctttttcgat ttcaagacaa ggtgcggcaa
    14821 cgcctggtga gtaggccagc gccagatcgc gctgcgttgc cagaggcttg gttggagaaa
    14881 cctggatttt ccctggaact ggaaattcat ggaaatcaag tgcactttgt tttaactggt
    14941 catccatttg gttgttcctt tcacgtaacg ttcacaaata aagtgtgtgg gcaacagccc
    15001 ctgcccacaa cgtggcgcac attattaccc tgccggagtc tacagacttt gagcaagtcc
    15061 aaactctcac cattaatata atgttttggt aataatccta taacactgat gttacctgct
    15121 taatccagca ataccatgcc tgtctgctat gcttttttga tgcgtttagc gaaatttctc
    15181 agaagtgtga attaacgcac tcatctaaca ctttactttt caaggagtat ttcctatgaa
    15241 cgagttagac ggcatcaaac agttcaccac tgtcgtggca gacagctgcg atattgagtc
    15301 cattcgccat tatcatcccc aggatgccac cactaatcct tcgctgttac tcaaggctgc
    15361 cggattatca caatatgagc atttaataga cgatgctatc gcctggggta aaaaaaatgg
    15421 caagacccag gaacaacagg tggtcgcagc gtgtgacaaa ctggcggtca atttcggtgc
    15481 tgaaattctc aaaatcgtac ccggttgcgt gtcaacagaa gttgatgcac gcctctcttt
    15541 tgataaagaa aagagtattg agaaggcgcg ccatctggtg gacttgtatc agcaacaagg
    15601 cgttgagaaa tcacgcattt tgatcaaact ggcttcgacc tgggaaggaa ttcgcgcggc
    15661 cgaagagctg gaaaaagaag gtattaactg caacctgacg ctgctgtttt cttttaccca
    15721 ggcgcgggcc tgtgcggaag caggcgtttt tctgatttcg ccgtttgtag ggcgtattta
    15781 tgactggtat caggcacgca agccgatgga cccgtatgtg gtggaagaag atccgggcgt
    15841 taaatcggtg cgcaatatct acgactacta taagcaacac cactatgaaa ccattgtgat
    15901 gggcgcgagc ttccgtcgca ccgaacaaat cctcgcctta accggctgcg atcgactgac
    15961 tatcgcaccg aatttgctga aggagctgca ggaaaaagtt tctccagtgg tacgtaaatt
    16021 aatcccgcct tctcagacgt tcccacgccc agcacccatg agcgaagcgg agttccgttg
    16081 ggagcacaat caggatgcga tggcggtaga aaaactgtct gaaggcattc gtctgttcgc
    16141 tgttgatcaa cgcaaactgg aagatcttct tgccgccaaa ctataaacca gccacggagt
    16201 gttatatgtc ccgaaaagac cttgccaatg cgattcgcgc actcagtatg gatgctgtac
    16261 aaaaagccaa ttctggtcat cccggcgcgc cgatgggcat ggctgatatt gccgaagtgc
    16321 tgtggaacga ttttcttaaa cataatccta ccgacccaac ctggtatgat cgcgaccgct
    16381 ttattctttc caacggtcac gcgtcgatgc tgctctacag tttgctacat ctgaccggtt
    16441 acgacctgcc gctggaagaa ctgaagaact tccgtcagtt gcattcgaaa accccaggcc
    16501 acccggagat tggctatacg ccaggcgttg aaaccaccac cggcccgctt ggacaaggtt
    16561 tggcgaacgc cgtcgggctg gcgatagcag agcgtacact ggcggcgcag tttaaccagc
    16621 cagaccatga gatcgtcgat cacttcacct atgtgtttat gggcgacggc tgcctgatgg
    16681 aaggtatttc ccacgaagtc tgttcgctgg caggcacgct gggactaggc aagctgattg
    16741 gtttttacga tcacaacggt atttccatcg acggtgaaac agaaggctgg tttaccgacg
    16801 atacggcaaa acgttttgaa gcctatcact ggcatgtgat ccatgaaatc gacggtcacg
    16861 atccgcaggc ggtgaaggaa gcgatccttg aagcgcaaag cgtgaaagat aagccgtcgc
    16921 tgattatctg ccgtacggtg attggctttg gttcgccgaa taaagcaggt aaggaagagg
    16981 cgcacggcgc accactgggg gaagaagaag tggcgctggc acggcaaaaa ctgggctggc
    17041 accatccgcc atttgagatc cctaaagaga tttatcacgc ctgggatgcc cgtgaaaaag
    17101 gcgaaaaagc gcagcagagc tggaatgaga agtttgccgc ctataaaaag gctcatccgc
    17161 aactggcaga agagtttccc cgacggatga gcggtggttt accgaaggac tgggagaaaa
    17221 cgactcagaa atatatcaat gagttacagg caaatccggc gaaaatcgct acccgtaagg
    17281 cttcgcaaaa tacgcttaac gcttacgggc cgatgctgcc tgagttgctc ggcggttcgg
    17341 cggatctggc tcccagcaac ctgaccatct ggaaaggttc tgtttcgctg aaggaagatc
    17401 cggcgggcaa ctacattcac tacggggtgc gtgaatttgg catgaccgct atcgccaacg
    17461 gcatcgcgca ccacggcggc tttgtgccgt ataccgcgac gttcctgatg tttgttgaat
    17521 acgcccgtaa cgccgcgcgg atggcggcac tgatgaaagc gcggcagatt atggtttata
    17581 cccacgactc aattggcctg ggcgaagatg gtccgacgca ccaggctgtt gagcaactgg
    17641 ccagcctgcg cttaacgcca aatttcagca cctggcgacc gtgcgatcag atggaagcgg
    17701 cggtgggctg gaagctggcg gttgagcgcc acaacggacc gacggcactg atcctctcaa
    17761 ggcagaatct ggcccaggtg gaacgtacgc cggatcaggt taaagagatt gctcgtggcg
    17821 gttatgtgct gaaagacagc ggcggtaagc cagatattat tctgattgcc accggttcag
    17881 agatggaaat taccctgcaa gcggcagaga aattagcagg agaaggtcgc aatgtacgcg
    17941 tagtttccct gccctcgacc gatattttcg acgcccagga tgaggaatat cgggagtcgg
    18001 tgttgccttc taacgttgcg gctcgcgtgg cggtggaagc aggtattgcc gattactggt
    18061 acaagtatgt tggtctgaaa ggggcaattg tcgggatgac gggttacggg gaatctgctc
    18121 cggcggataa gctgttcccg ttctttggct ttaccgccga gaatattgtg gcaaaagcgc
    18181 ataaggtgct gggagtgaaa ggtgcctgat ggtgatcgcc ggatgctgta tgttcccctc
    18241 accctaaccc tctccccaga agggcgaggg gacggtccgt gcgcttttta gggccgcatc
    18301 tgcgattttg tttaacgggt gatccacaac gtgggcctgg cgtctggccc atgccagtta
    18361 tcgcaggtag gttctgcggc gtaacgcacc aggcgaaaac gctgaccgtc aaagcgccag
    18421 cgcgcctgaa tgccacaatc gcttaatccg cgccctttcg ctaaggtcac cagttcacgc
    18481 gatttctcat caaatgttgc gttcatcagt tccagttcat tcgtctcctg gccgttgttg
    18541 aacggcaaac gcaaccgaac cgggcgcgag gctagcggct ttttacgcga cacaatccat
    18601 gccaaatcaa tggtgttata ggcccctgcc tcacagctaa tcatcatcag tgctttatca
    18661 tcggtcagcg cagtgacatt cacctcacga cgtaacggat caagcgagca gcgcagacca
    18721 ttcatccgcc agtttccata atccagcaaa tcgttacgtt cttcgagtga gagtggcgtc
    18781 ggcgttgggt taaccaccgc gacctctttc agcgcaggcg cgggcggtac gctgagaggc
    18841 ggttcgtccc ctttcttgat ccacgcggtt tcactgccaa cgcgcttttg ctgagcatca
    18901 ataaacaaca acgccgcttt taagccgctc agagaaatgg tctgattgcc gtcgcgtaag
    18961 gtgatcgcct tcccttcctg aattatctgc aaaaacgcgg tgatggttgc cgtatcatcg
    19021 gttactaata accatggtga aatccgccac ttgtcaccac ttaatgccaa cggctcacca
    19081 tctaatagca gccgtggcgc tatctcccct tcagacgcat ccggcgactt caacccaccg
    19141 cgctcaatac gtaaaacggc atcggtatgc gccccagcgc tgcggctcag ggtcatcacc
    19201 agtccattat gatcgcccgt attgcgcgcc acacaaaaat tttgattatt acaggtgacc
    19261 tgccagtcgg aaaacgcccg ttgcgctggt gccgcccaca ccaaagacgt cggcaacaaa
    19321 gcgaaaaaaa agagaaggaa aatgcgatag cgcatggacg gtacgacccc agaagttcaa
    19381 acaaacagca caagtcgtat cttcgttgcg caggtagcgc ggctcaatcg gatttgtcgg
    19441 attgagccaa taataaacaa cgaaatatta ttgctatttt tcagtccatt aaatgtgaca
    19501 tttgcaaata gttaagcaat aacaccgtct taccgtcacg tatctcgccg gttttgatca
    19561 tctccagcgc ctggctgaac ggcagctcga gcacttcaat atcttcatct tcaacaccgc
    19621 caccggcgtt agcgcgctga ttgtcactgt attcggcgat aaaaaagtgg attagctcag
    19681 tcacaccgcc aggcgacata tacagttcaa ataatttgcg cacttcgccc acttcatagc
    19741 ccgtctcttc aatcgcttct ttacgaatgc acacttccgg ttcgtcgtta tccagcagcc
    19801 cggcgcaggt ttcaatcagc tgcccgcttt cattgccatt aacccaggta gcgacacgga
    19861 actgacgaat cagaaccacg ctctttttct tcgcgttgta caggaggatc gtcgcaccat
    19921 taccgcgatc gtaaacttca cgtttatggc ggataacttc gccatctttg cgcgtgagat
    19981 cgtaagtaat gttgtgcagg gtgaaatagt tatcggagag aattttgtct ttaatgaggg
    20041 tgatttgttg cgtcataccg actccacagc gcgaaatgaa caattatatt acgctgtgaa
    20101 gtcgggtttg tctgcgcagg ctatcagtga gatttgactg attttacccc cagccagtca
    20161 ataatcccct gtgccgcatg acgtccttct gccattgcgg taaccaccag atccgcacca
    20221 cgcacggcgt cgccaccggc gaagattttc ggattggtgg tctggtaacg gtactggctt
    20281 tccacatccg cgatgatgcg gccccatttg tctaccgtta caccgtgcga ctccagccac
    20341 ggcatcccgt gtggattgaa gccaaacgcc ataatcaccg catcggctgg catgacaaat
    20401 tcactgccct caatcggtac aggtcgccga cgcccctggg catccggctc gccaagacgc
    20461 gtgcgcagga aacgaattcc gcaaacgtga ccttgctcat tcagctcaag cgccaccggc
    20521 tggacgttaa attcgaagtt ggccccctct tcgcgggcgt tcttcacctc tttcttcgag
    20581 cctggcatgt tagcttcatc acgacgataa gcgcaggtga cgttactcgc gccgtggcgc
    20641 agcgcagtac gcacacagtc catcgcggtg tcgccgccgc ccagtaccac gacgttaagt
    20701 ccggcggtat tgataaacgg ctcttccggt agctcttcga gccccatcac ctgtttagtg
    20761 ttggcaatga ggaacggcag cgcgtcataa acgcccggcg catcttcatt gggtaaaccc
    20821 gctttcatgg aacggtaagt gcctacgcca acgaagaccg cgtcgtattg ttccaaaagc
    20881 aaatccaaag agacatcttt acccacttca caattgagtt cgaagtgaat ccccatcgcg
    20941 ctgaagattt cccgacggcg tgccagcagg gatttatcca gtttgaaaga aggaatgccg
    21001 aaagtgagca agccaccgat ttctggatgg cgatcgtaca ccgtcaccgc cacgccattg
    21061 cgggtcagaa catccgcaca ggccagccct gccggacctg ccccgataat cgccacccgc
    21121 ttgtccactt tggtgacatg acttaagtca ggacgccaac ctttcgccaa cgcctgatct
    21181 gaaatatagc gttcaatgtt gccgatagtt accgcgccgt gctcatcgcg aatagtacag
    21241 gcaccttcac acaaacggtc ttgcggacaa acgcgtccgg taatttccgg cagggtgttg
    21301 gtctggtgag aaagctcgac ggcggcgtcg atgtttccgg ctttcaccag ttcaatccac
    21361 tgcggaatat ggttatgcag cgggcaggtc cattcacaaa cgctgtgctc gccgaactta
    21421 aggcagcgcg aggcttcccg ttgtgcctgg tcggcgcgaa atggcagata aatttcatca
    21481 aaaccggttt tgcgcgcttc aatcgccagt ttatccggct cgccacgcgc gggcgttgcc
    21541 tgcatttgtt cgactttact cattaccggc atttcttgtg cagcggtact ggcatgccac
    21601 ggttgatgct cctgacgtgc ggtgcgcaag cggcgggatt tcgccatgcc ggagagtgcg
    21661 acgtcagtga ccagttgcag cgcgtccgcc gggcaattct cgacacaggc aggaccgttt
    21721 tcgcgccctg cacaaaggtc gcatttatgc gccgtggctt ttacttttcc tgccgcgacg
    21781 ggtgtcagga cgatttgcat cgtaccaaac ggacaggcca ccacgcagga tttacagcca
    21841 atacactttt gctgattgac ctgaatgctg tcatcaacgt ggctgattgc gccattaggg
    21901 cagctacggg cgcagggcgc atcttcacaa tggtgacagg tcactgcact acgttgctgc
    21961 tgatgtttga taaccgtaat tcggggatga aaatggtgtt ggctcaggac atgttgctca
    22021 tcattgtgag ccatgacaca ggcgatttca caagcatgac aacccagaca ttgctgactg
    22081 ttggccataa taaaacgatt cataacgacc ttcttttttg gttgtaaaaa ccttattctt
    22141 tatatgagtg ttgttattac ccgacttaca ggggaaccgg caatgttcat gtgcccagaa
    22201 taagtaacta tttcgctata aactgtggca gatcaaataa tctcatcact gactaaattg
    22261 cgtttcagcg aactggaaca ttaatgattt tttgtggaga agacgcgtgt gattgttaaa
    22321 cgacccgtct cggccagtct ggcccgggcc tttttttaca ttgtgctgct gtcgattctt
    22381 tccacgggta tcgctctgct aactctggcg agcagtttgc gcgacgctga ggctatcaat
    22441 attgccggat cgctgcgtat gcagagttac cgcctgggct acgacttgca aagtggcagt
    22501 ccacaactca atgcacatcg ccagttgttt cagcaggcac tgcattcacc ggtattaacc
    22561 aacctcaacg tctggtatgt gccagaagca gtaaaaactc gctatgcgca tctgaatgcc
    22621 aactggctgg agatgaataa tcggctcagc aagggcgatt tgccgtggta tcaggccaat
    22681 attaataatt atgttaatca gatagacctg ttcgtgctgg ctttacagca ctacgctgaa
    22741 cgcaaaatgc tgctggtggt ggcgatttcc ctggctggcg gtatcggtat tttcacgctg
    22801 gtctttttta ctttgcgccg catacgccat caggtggttg ccccgctgaa tcagctggtt
    22861 accgccagtc agcgtattga acacgggcag ttcgactcgc cgccgctgga taccagtctg
    22921 ccgaatgagc ttggtctgct tgcaaaaacc tttaaccaaa tgtcgagcga gctgcataaa
    22981 ttgtaccttt cgctggaggc gtcagtagaa gaaaagaccc gcgatctcca cgagaccaag
    23041 cgtcgtctgg aggtgttgta tcagtgcagt gttcccaggc gctgaacacc agccagattg
    23101 atgtgcattg tttccgccat attttgcaga ttgttcgcga caatgaagca gctgaatatc
    23161 tggagctgaa cgttggggat aactggcgga ttagcgaagg gaagccaaac cctgagctgc
    23221 caatgcagat tttaccggtg acaatgcaag agacggttta cggcgaactg cactggcaaa
    23281 atagtcacgt ttcatcatca gaaccgctgc ttaacagtgt ttcatcgatg ctgggacgcg
    23341 gtttgtactt taatcaggcg cagaagcatt ttcagcagtt attgttgatg gaagaacgtg
    23401 cgaccatcgc ccgcgaattg cacgactcgc tggctcaggt gctttcttac ttacgtatcc
    23461 agttgacgtt actgaagcgt tcgataccgg aagataacgc caccgcacaa agtatcatgg
    23521 ccgatttttc ccaggcgttg aatgatgctt atcggcagtt acgcgagctg ttgactactt
    23581 tccgcctgac gctgcagcag gcggatctcc cctccgcgtt gagggaaatg ctggatacgt
    23641 tacaaaatca aaccagcgct aaactgaccc tcgactgccg tctgccaacc ctggcactgg
    23701 atgcgcaaat gcaggtgcat ttgttgcaaa ttattcgcga agcggtgctg aatgcgatga
    23761 agcacgccaa cgccagcgaa atcgccgtca gttgcgtcac cgcgccggac ggcaatcaca
    23821 cggtttatat ccgtgataac gggattggta tcggtgaacc gaaagaaccc gaaggtcatt
    23881 atggactgaa tatcatgcgc gaacgcgcgg aacggctggg cgggacgctg actttttcac
    23941 aaccttccgg cggcggcacg ttagtgagta ttagctttcg ctccgcagag ggtgagggga
    24001 aggggaacac gccggctgag gtgtaaataa ttaccctcac accggccctc tccctttaaa
    24061 ggcgagaggg aaaaacgtgc ccatattgac ctgtaagcga gtgtgtcagc aatctcatga
    24121 taatttacat taactccttt ttttctccac gattggctcg taccttgccg ctacagtgaa
    24181 gcaagtcaag cctacaacaa tacgcagaaa cacgaggtcc tcttttaatg gcgaatttct
    24241 ttattgatcg ccccattttt gcctgggtgc tgacaatcct gttgtgtctg acaggtaccc
    24301 tggcgatttt ttcattgccc gttgaacaat accccgatct cgcgccaccg aatgtgcgag
    24361 tgaccgctaa ctatcccggc gcatcggccc agacgctgga aaacaccgtg acccaggtta
    24421 tcgagcaaaa tatgaccggc ctcgataatc tgatgtatat gtcatctcag agcagtggca
    24481 ccggtcaggc atctgtcact ttaagtttta aagcaggcac cgatccggac gaagccgtgc
    24541 agcaagtaca aaaccagctg caatcagcca tgcgaaagtt accgcaggcg gtgcaaaatc
    24601 agggcgtgac ggtgcgtaaa accggcgata ccaacattct gaccattgcc ttcgtctcta
    24661 ccgatggctc gatggataaa caggatattg ctgattatgt tgccagtaat attcaggacc
    24721 cgttaagccg cgtgaatggc gtcggggata tcgatgccta tggttcgcaa tattccatgc
    24781 gtatctggct ggacccggcg aaactcaaca gtttccagat gacggctaaa gatgtcactg
    24841 atgccattga gtcacagaac gcgcagattg cggttgggca acttggtggt acaccttccg
    24901 tcgataagca ggcgctcaac gccaccatta acgcccagtc actgctgcaa acaccagaac
    24961 agttccgcga tatcaccttg cgggtgaatc aggacggctc agaggtaagg ctgggcgatg
    25021 tcgccaccgt cgaaatgggg gcggagaaat acgattatct tagccgcttc aatggtaagc
    25081 cagcctccgg gttgggggta aaactggcct ccagcgcaaa cgaaatggcg acagcggagc
    25141 tggtgctcaa tcgtctcgac gagctggcgc agtatttccc gcacggactg gaatacaagg
    25201 tggcgtatga aaccacctcg tttgttaaag cctccattga agacgtggtg aaaacgctgc
    25261 tggaagctat cgctctggtt ttcctcgtta tgtatctgtt cctgcaaaac ttccgcgcca
    25321 cgctgatacc cactatcgcc gtgccggtgg tgttgatggg aaccttctcc gtactttacg
    25381 ccttcggtta cagcgtcaac accttaacca tgttcgcgat ggtgctggcg atcggtctgc
    25441 tggtggatga cgccatcgtg gtggtggaaa acgtcgaacg tattatgagt gaggaaggac
    25501 tcactcctcg cgaagccaca cgtaaatcga tggggcagat ccagggggca ctggtcggga
    25561 ttgcgatggt gctttcggcg gtatttgtac caatggcctt cttcggcggc accaccggtg
    25621 ccatctatcg ccagttctct attaccattg ttgcggcgat ggtgctgtca gtactggtag
    25681 cgatgctcct cactccggct ctgtgtgcca cgctacttaa gccactgaaa aaaggtgagc
    25741 atcacgggca aaaaggtttt tttgcctggt ttaaccagat gtttaaccgc aacgccgaac
    25801 gctacgaaaa aggggtggcg aaaattctcc accgtagcct gcgctggatt gtgatttatg
    25861 tcctgctgct tggcggcatg gtgttcctgt tcctgcgttt gccgacgtcg ttcttaccgc
    25921 tggaagaccg gggcatgttt actacctcgg tacaattgcc cagcggttca acccaacaac
    25981 agaccctgaa agtcgttgag caaatcgaga aatattactt cacccatgaa aaagacaaca
    26041 tcatgtcggt gtttgccacc gttggttctg gccctggggg taacgggcaa aacgtggcga
    26101 gaatgtttat ccgcctgaaa gactggagcg aacgcgacag taagaccggc acctcgtttg
    26161 ccattatcga gcgtgcaacg aaggcgttta accaaattaa agaagctcgc gttatcgcca
    26221 gcagcccacc agcaattagc ggtcttggta gttctgcagg ttttgatatg gagttgcagg
    26281 accacgctgg agcgggtcac gatgcgctga tggcagcacg taatcagttg ctggcgctgg
    26341 cggcggaaaa cccggagcta acccgtgtgc gccataacgg cctcgatgac agtccgcagt
    26401 tgcagattga tatcgaccag cgtaaagctc aggcgctggg cgttgatatc gacgatatta
    26461 acgacacact gcaaaccgcc tggggttcga gctatgtgaa tgactttatg gatcgcggtc
    26521 gcgtgaagaa agtctatgtg caggcagctg cgccgtatcg catgctgcca gatgacatca
    26581 atctctggta tgtccgaaat aaagatggcg gcatggtgcc cttctctgct ttcgcgacct
    26641 cacgctggga aacaggctcg ccgcgtctgg aacgctataa cggttattct gcggttgaga
    26701 ttgttgggga agccgcaccg ggggtcagta ccggtacggc gatggatatt atggaatcgt
    26761 tagtgaagca gctgccaaac ggctttggtc tggagtggac ggcgatgtcg tatcaggagc
    26821 ggctttccgg cgcgcaggct ccggcgctgt acgccatttc cttgctggtg gtattcctgt
    26881 gtctggcggc attgtatgaa agctggtcgg taccgttctc ggtaatgctg gtcgtgccgc
    26941 tgggggtaat cggcgcgctg ctggcaacct ggatgcgcgg gctggaaaac gacgtttact
    27001 tccaggtggg cctgttaacg gtcattggtt tatcggcgaa aaacgccatc ctgatcgtcg
    27061 agtttgctaa cgagatgaac caaaaaggcc atgacctgtt tgaagcgacg ctccacgcct
    27121 gccgtcagcg tttacgcccg attctgatga cctcgctggc atttatcttc ggcgtattgc
    27181 caatggcaac cagcacgggt gccggttccg gtggtcagca tgcggtgggt actggcgtaa
    27241 tgggcgggat gatttcagct actattctgg ctatttactt cgtgccgctg ttctttgtgc
    27301 tggtgcgccg ccgcttcccg ctgaagccgc gcccggaata agcaataaaa aaggcgacat
    27361 gccaatgtgt cgcctttttc aactttccga ttaagaacct gctcagcggg ttcttgctgt
    27421 ttatactttg tctcaggaat tacttacgaa gcataacttc gataaagtct ttccagttcc
    27481 ccagttcacg ttcaatcata acaacctctc ttataattat gggtattcta cggaaacaat
    27541 ataccgtggt gaagctaatt tactcgattg ctgcgatgac tacctccggg ggacaaacct
    27601 tatgtaaata ctatggtcct gcggtgataa tttgtatgtg atacacagca acatttcgag
    27661 atattcatac ggcatctaat actgatttaa ttctggttaa aatacagaca gataacaaga
    27721 tgaatattct taatgtttac gttaaaaatg tttaatatta tttaatagtt gttaatttga
    27781 atacttcgat aatgttatat tttctgataa tcatttgcag gcaaaatgtt ttcaccctta
    27841 aatgagtatt tattctcata aatcgaaaaa ggattcatta tggttacact ttacggcatc
    27901 aaaaattgtg acaccattaa aaaggctcgc cgttggctgg aagccaataa catcgactat
    27961 cgttttcatg attaccgcgt cgatgggctg gacagcgaat tattgaacgg ttttatcaac
    28021 gaattaggct gggaagcgtt actcaacacc cgtggtacaa cctggcgtaa actggacgaa
    28081 actacccgca ataaaatcac cgatgcggcc tctgcggcgg cattaatgac tgaaatgcct
    28141 gcaattatca aacgtccatt gctctgcgcg cctggtaagc ctatgctgct gggtttcagt
    28201 gattccagtt atcagcaatt tttccatgag gtgtagtcta tgtcgtgccc ggttattgag
    28261 ctgacacaac agcttattcg ccgcccttcc ctgagtcctg atgacgcagg atgtcaggct
    28321 ttgttgattg aacgtttgca ggcgatcggt tttaccgttg aacgcatgga ttttgccgat
    28381 acgcagaatt tttgggcatg gcgtgggcaa ggtgaaacgt tagcctttgc cgggcatacc
    28441 gacgtggtgc cgcctggcga cgccgatcgt tggatcaatc cgccgtttga acccaccatt
    28501 cgtgacggca tgttattcgg gcgcggtgcg gcagatatga aaggctcgct ggcggcgatg
    28561 gtggtagcag cagaacgttt tgtcgcacaa catcccaacc atacggggcg actggcattt
    28621 ctgatcactt ctgatgaaga agccagtgcc cacaacggta cggtaaaagt cgtcgaggcg
    28681 ttaatggcac gtaatgagcg tctcgattac tgcctggtcg gcgaaccgtc gagtatcgaa
    28741 gtggtaggtg atgtggtgaa aaatggtcgt cgcggatcgt taacctgcaa cctcaccatt
    28801 catggcgttc aggggcatgt tgcctaccca catctggctg acaatccggt acatcgcgca
    28861 gcacctttcc ttaatgaatt agtggctatt gagtgggatc agggcaatga attcttcccg
    28921 gcgaccagta tgcagattgc caatattcag gcgggaacgg gcagtaacaa tgttattccg
    28981 ggtgaactgt ttgtgcagtt taacttccgc ttcagcaccg aactgactga tgagatgatc
    29041 aaagcgcagg tacttgccct gcttgaaaaa catcaactgc gctatacggt ggattggtgg
    29101 ctttccgggc agccattttt gaccgcgcgc ggtaaactgg tggatgcggt cgttaacgcg
    29161 gttgagcact ataatgaaat taaaccgcag ctactgacca caggcggaac gtccgacggg
    29221 cgctttattg ctcgcatggg ggcgcaggtg gtggaactcg ggccggtcaa tgccactatt
    29281 cataaaatta atgaatgtgt gaacgctgcc gacctgcagc tacttgcccg tatgtatcaa
    29341 cgtatcatgg aacagctcgt cgcgtgatga gtggttctgc aagaggaaat aagcatggac
    29401 tggctggcta aatattggtg gattctggtg attgtctttt tggtaggcgt cctgctgaac
    29461 gtgattaaag atctcaagcg cgtcgaccat aagaaatttc tcgccaacaa gccggagctt
    29521 cccccgcatc gtgatttcaa cgataagtgg gacgatgacg acgactggcc gaaaaaggat
    29581 caaccgaaga agtaagctga gtgctcaacc gtaggccgga taaggcgtta acgccgcatc
    29641 cggcaatggt gaacgatccc tgatgcgacg ctcacgcgtc ttatcaggcc aggtaatctg
    29701 tcttttacat catttcaatc acatcatcat cgccaggctt accgccactt agcgcttggt
    29761 cgaaataatg tttcggaatg gtataacgca agtgatccag ggcaaattgc atactgcgat
    29821 tgtcgatcgc atggcctaaa tcctccacga tatccagcgt cacgtcacca ccggcactga
    29881 ttaacgcttc ctgcgccgca actgcatgcg ccagatcgat aaccggatct tcaccaccat
    29941 gaatcaagtg aattgtcgtg gctgtagacg cggtctccgg caggctcgca tagcgtccgt
    30001 taaaggcaat gacgcgcgaa gcaaggcccg gttcggcttt aatgctctcc agcgccataa
    30061 tcgcgccttg tgagaaacca attagcgccg tggcgttagc ccctactccg ctctgtttct
    30121 gccagtagcg taccgtttca ataaacgtcg gcatgatggc atccacacgc gcctggcgat
    30181 tatcttccgt aataccctgc actgaaaacc actgacgccc cgccggatta ccgctcggct
    30241 ccgcgccacc gacactcacc accagcgcat caggaaacag cggtgcaaac cagctgccta
    30301 tttcccccat tgccaccggg ttatccccga caccgtggaa aagcagtaac aactgttgtg
    30361 caggtttatc cgggctttga acaacaaaat ggtcatgttt catggcgaac tccttaactg
    30421 atgtcattaa ttttacgccg ctgactcaca atgaccatgg gagaaaattg aaggagtcag
    30481 tgaaaaaatt gccattgcgt tatgcgatcg cgcagacgct cagtgcgaac ctcatttagt
    30541 gcgaacagcg cctgcgcggc ctcttcccgc tggcgaacca gtaacatctt gcggcctgaa
    30601 agtttaagtg tggtacataa ctgcgcgtca ctggcgtttt tctgtaaacg cccacgcagc
    30661 gccggtaatg ccagttcact ggtttgtagc agacgcaata agcaacctaa cgatgttaat
    30721 agcggacgat gagcgaaagc aaaaccggcc agttcaagcc agtcgtcgtc agaaaggatg
    30781 gcatcgttta gtggatcaac aggaagcatt tcgccattcc actgcgcgag agcttgcgca
    30841 tcgcgacgta aacggtaatg ctcacgttca gccagctgtt tacccgcatc actcatcggt
    30901 aacagcgcca tcgccgtata gcaaccgctg ctggcttccc gatgattacc catccgcacc
    30961 agcacaaaac cgcagcgttg ccagaaacgc cataactccc cagtgtaacc aaaactcacc
    31021 gaaagatagt cgaggtcatg cgtatattgc aaagctccag caataagttg tcgccctgtg
    31081 ccttcccgct gacgagccgg atgaactgct atccggctaa cccgccttcc gcgcaatgtc
    31141 gccgccagtg gattgctgcc gtgcgccgcc agcgactggg ccaccagatt accccgcggg
    31201 cgacgaaaac ctgcccatac cgcctgactg agttcttgag ataatccgcc ctcatccacc
    31261 agccacaacg ccccggcaat ctcgttttcg ccagccgcct gtaaaaaatg ttgccctggt
    31321 gcatccatca tccggcgtaa atccagcggc gaggtccggt agtgcgcacc agacaataac
    31381 tgataaacct ttaacggcgt ttctggctcg cttcgccata acgtttgttc aaatgcggaa
    31441 atgacgatat tgccttgtgg tgtatgggtg aagttttcat cgttaaaaac cagtgcctca
    31501 ctaaccattt tttccagcgg gcatccctgt gcccagcgga tcggctgttg cagttcaaaa
    31561 cggtgtaaat gcggaaagcg agcgcaaaat ttcagcaaaa aaccacgtcc ggtaccttcg
    31621 tagccctgca ccgtagtggt taacaacgtt cgaggaaaac gcgataccag ttgatgca
//
LOCUS       DADLTY010000028        31028 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.028, whole genome shotgun sequence.
ACCESSION   DADLTY010000028 DADLTY010000000
VERSION     DADLTY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.028"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(435..641)
                     /locus_tag="JN334_001088"
     CDS             complement(435..641)
                     /locus_tag="JN334_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HAY7437313.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="JN334_001089"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="JN334_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAY7437314.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="JN334_001090"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="JN334_001090"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HAY7437315.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4786
                     /gene="kdpB"
                     /locus_tag="JN334_001091"
                     /pseudo
     CDS             2739..4786
                     /gene="kdpB"
                     /locus_tag="JN334_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /locus_tag="JN334_001092"
     CDS             4795..5367
                     /locus_tag="JN334_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HAY7437316.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8037
                     /gene="kdbD"
                     /locus_tag="JN334_001093"
                     /pseudo
     CDS             5360..8037
                     /gene="kdbD"
                     /locus_tag="JN334_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
     gene            8034..8719
                     /gene="kdpE"
                     /locus_tag="JN334_001094"
                     /pseudo
     CDS             8034..8719
                     /gene="kdpE"
                     /locus_tag="JN334_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8994..9098
                     /locus_tag="JN334_001095"
     CDS             8994..9098
                     /locus_tag="JN334_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HAY7437317.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9409..11606
                     /gene="speF"
                     /locus_tag="JN334_001096"
                     /pseudo
     CDS             9409..11606
                     /gene="speF"
                     /locus_tag="JN334_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11603..12922
                     /gene="potE"
                     /locus_tag="JN334_001097"
     CDS             11603..12922
                     /gene="potE"
                     /locus_tag="JN334_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HAY7437318.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12978..14618)
                     /gene="pgm"
                     /locus_tag="JN334_001098"
     CDS             complement(12978..14618)
                     /gene="pgm"
                     /locus_tag="JN334_001098"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HAY7437319.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14644..15189)
                     /gene="seqA"
                     /locus_tag="JN334_001099"
     CDS             complement(14644..15189)
                     /gene="seqA"
                     /locus_tag="JN334_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HAY7437320.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15374..16138
                     /gene="ybfF"
                     /locus_tag="JN334_001100"
     CDS             15374..16138
                     /gene="ybfF"
                     /locus_tag="JN334_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7437321.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16209..16571
                     /gene="ybfE"
                     /locus_tag="JN334_001101"
     CDS             16209..16571
                     /gene="ybfE"
                     /locus_tag="JN334_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HAY7437322.1"
                     /translation="MYYGALSIRAEAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            16711..17241
                     /gene="fldA"
                     /locus_tag="JN334_001102"
     CDS             16711..17241
                     /gene="fldA"
                     /locus_tag="JN334_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HAY7437323.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17451..17537
                     /gene="uof"
                     /locus_tag="JN334_001103"
     CDS             17451..17537
                     /gene="uof"
                     /locus_tag="JN334_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HAY7437324.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17530..17976
                     /gene="fur"
                     /locus_tag="JN334_001104"
     CDS             17530..17976
                     /gene="fur"
                     /locus_tag="JN334_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HAY7437325.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18060..18386)
                     /locus_tag="JN334_001105"
     CDS             complement(18060..18386)
                     /locus_tag="JN334_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437326.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18423..19829)
                     /gene="chiP"
                     /locus_tag="JN334_001106"
     CDS             complement(18423..19829)
                     /gene="chiP"
                     /locus_tag="JN334_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HAY7437327.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20408..22072)
                     /gene="glnS"
                     /locus_tag="JN334_001107"
     CDS             complement(20408..22072)
                     /gene="glnS"
                     /locus_tag="JN334_001107"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAY7437328.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22275..24221)
                     /gene="nagE"
                     /locus_tag="JN334_001108"
     CDS             complement(22275..24221)
                     /gene="nagE"
                     /locus_tag="JN334_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HAY7437329.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24554..25354
                     /gene="nagB"
                     /locus_tag="JN334_001109"
     CDS             24554..25354
                     /gene="nagB"
                     /locus_tag="JN334_001109"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY7437330.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25414..26562
                     /gene="nagA"
                     /locus_tag="JN334_001110"
     CDS             25414..26562
                     /gene="nagA"
                     /locus_tag="JN334_001110"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY7437331.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26571..27791
                     /gene="nagC"
                     /locus_tag="JN334_001111"
                     /gene_synonym="nagR"
     CDS             26571..27791
                     /gene="nagC"
                     /locus_tag="JN334_001111"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HAY7437332.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27839..28591
                     /gene="nagD"
                     /locus_tag="JN334_001112"
                     /pseudo
     CDS             27839..28591
                     /gene="nagD"
                     /locus_tag="JN334_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            28988..30652
                     /gene="asnB"
                     /locus_tag="JN334_001113"
     CDS             28988..30652
                     /gene="asnB"
                     /locus_tag="JN334_001113"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HAY7437333.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
BASE COUNT         7400 a         7835 c         8073 g         7720 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggctatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggtgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggaattacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggagccg
     7201 gggaatctgc gccagttgat gatcccggaa cagcagcgcc tgctggagac ttttacgctg
     7261 ttagtcgcca atgcccttga gcggctgacg ctaaccgcca gcgaagaaca ggcgcggatg
     7321 gcaagcgaac gtgaacagat ccgcaacgcc ctgctggcag cgctttcgca tgatttacgc
     7381 acgccgctta cggtgctgtt tggtcaggca gaaatcttaa cgctcgatct ggcaagcgaa
     7441 ggatcacccc acgcccgcca ggccagcgag attcgtcagc atgtgctgaa cactacccga
     7501 ctggtgaata atctactgga tatggcgcga attcagtccg gcggctttaa tttgaagaaa
     7561 gagtggttaa cgctggaaga agtagtcggc agcgcgctgc aaatgccgga accgggttta
     7621 tcgtcgccca tcaatctttc tctgccagaa ccgctgacct taatccacgt tgacgggcca
     7681 ctctttgaac gggtgctgat taatctgctg gagaacgcgg tgaaatatgc gggtgcgcag
     7741 gccgaaattg gtatcgatgc ccacgttgag ggcgaaaatc tacaactgga tgtctgggat
     7801 aacggccccg gtcttccgcc aggccaggag cagacgatat ttgataagtt tgctcgcggg
     7861 aataaagagt cggcggtgcc gggtgtcggg cttgggttgg caatttgtcg ggcgattgtg
     7921 gatgtacacg gcggcaccat taccgcgttc aaccgaccgg aaggtggtgc ctgttttcgt
     7981 gttacacttc cccagcaaac cgctcctgaa cttgaagact ttcatgagga tatgtgacaa
     8041 acgttctgat tgttgaagat gaacaggcta ttcgtcgctt tctgcgcacg gcgctggagg
     8101 gcgacgggat gcgcgtcttt gaggccgaaa ctctgcaacg cggcttgctg gaagcggcaa
     8161 cccgtaagcc agatttgatt attctcgatc tcggcctgcc cgatggtgat gggattgagt
     8221 ttatccgcga cctgcgccag tggagcccgg tgccggtgat tgtgctttcc gcacgcagcg
     8281 aagagagcga caagatcgcc gcgctggatg ccggagcgga tgattatctg agtaagccgt
     8341 ttggtattgg cgaattgcag gcccgtctgc gcgtcgcttt acgccgccac tccgccactc
     8401 cgccaccgcc gcgcccgatc cgctggtgaa attttccggt gttaccgtcg atttagccgc
     8461 ccgcgtgatt caccggggtg aggaagaggt gcatctcaca ccaattgagt tccgcctgct
     8521 ggcggtgctg ctcaacaatg ccggaaaagt actcacccag cgccagctcc ttaaccaggt
     8581 gtgggggcca aacgcggtcg aacacagtca ctatttgcgt atttatatgg gacatctgcg
     8641 acaaaaactg gaacaggatc cggcccggcc tcgccacttc attactgaaa ccggtattgg
     8701 gtatcggttt atgctttgaa ttgttattca ataaattcgc ttaaatagtg attaatttat
     8761 tattaaacct gcgccgaata ttattatttg gcaggtttaa tttcttaacg caaatataaa
     8821 acacgaaatt acaacattta agtaacaaaa cagcatctaa tatggcgata ttcataatta
     8881 atgatttatt atctggggta tgatcgtttt ttgttgatct tcttcacaga ttatagccat
     8941 ttcatggata gaataactct accttcaact gacacagcaa gcggtaaagg taaatggaaa
     9001 ataataaccg cttaatgcct catataaggc ggacaaccca tatcatgaag tttgcccatc
     9061 gtaatagttt tgactttcat ttctttaatg cccgctagtc ttctgactaa agggcacccc
     9121 aacgtacagg tctccctgac tttaagcatt acaggttaat acctgtattc ctcggtgctc
     9181 atatactgct aaccctttta aactctaaat aattcgagtc gcagcacttg caacttgagg
     9241 tatgacgagt atagccagtt accgggctgg tctgggttat tgcatctgca aaaagcaaac
     9301 tactgattta tttatcagcg gtggagcttt gctttttttc cgacgtgatc gatttctcct
     9361 ttgagaaatt gaggacctgc tattacctga aataaagaga tgaataaaat gtcagaatta
     9421 aaaattgccg ttagccgttc ttgcccggat tgtttttcca ctcatcgtgc atgcgtgaat
     9481 attgacgaaa gtaattatat tgacgttgcc gccattattt tatcagtcaa tgatgttgaa
     9541 cgtggaaaac tcgataaaat agacgctact ggctatggca ttcctgtttt tattgcaacg
     9601 gaaaatgaag aacgtgtacc agcagaatat ctgccacgta tttctggtgt cttcgagcat
     9661 tgcgaatcac ggaaagagtt ttatggacgt cagttagaaa ccgctgctag ccattatgaa
     9721 acgcaactgc gcccgccctt cttccgcgca ctggtcgatt atgtcaatca gggcaatagc
     9781 gcttttgact gcccgggtca tcagggaggt gagtttttcc gtcgtcatcc ggcagggaat
     9841 cagttcgtgg agtacttcgg agaaacgctg ttccgttccg acttatgcaa cgcagacgta
     9901 gcaatgggcg atctgctgat tcacgaaggc gcgccatgca ttgcacagca acatgcggca
     9961 aaagtgttta atgccgataa aacatacttc gttctaaatg gcacttcatc ttctaacaaa
    10021 gtggttttaa acgccctgct aacaccgggt gatctggtgc tgtttgatcg caataaccac
    10081 aaatctaacc accacggagc gttgctacag gctggtgcaa caccggttta tctggaaacg
    10141 gcacgtaacc cgtatggctt tatcggtggc attgatgcgc actgttttga agaaagttac
    10201 ctgcgtgagc tgatcgcgga agtcgcaccg cagcgggcaa aagaggctcg tcctttccgc
    10261 ctcgctgtga ttcagttagg cacctacgac ggtacgattt ataacgcccg ccaggtggtg
    10321 gataaaattg gtcatctgtg tgactacatt ctgtttgact ccgcatgggt tggctatgag
    10381 caatttatcc cgatgatggc tgactgttcg ccgctgttgc tggatcttaa tgagaacgat
    10441 ccgggtattc tagttacgca atccgtacat aaacagcagg ctggtttttc tcaaacttca
    10501 caaatccata aaaaagacag ccacatcaaa gggcaacagc gttatgtacc gcacaaacgc
    10561 atgaacaacg cctttatgat gcacgcctcc accagcccgt tctatccgct gtttgccgca
    10621 ctggatatca acgccaaaat gcatgaaggt gtcagcggtc gtaatatgtg gatggattgt
    10681 gtggtaaatg gcattaatgc ccgcaaactg atcctcgata actgtcagca tattcgtccg
    10741 ttcgtacctg aactggtgga tggtaaaccc tggcagtcgt atgaaacagc gcaaattgcg
    10801 gttgatctgc gcttcttcca gtttgtacca ggggaacact ggcattcttt tgaaggctat
    10861 gcagagaatc aatactttgt cgatccttgc aaactgttgc tgacaacccc aggtattgat
    10921 gcacgtaacg gcgaatatga agcgttcggt gtacccgcga cgattcttgc taacttcctg
    10981 cgcgaaaatg gcgtagtgcc ggaaaaatgc gatcttaacg ccatcctctt cctgctgact
    11041 ccggcagaag atatggccaa acttcagcaa cttgtgccct gctggtacgc ttcgaaaaac
    11101 tgcttgagtc cgacgcgcca ttagcagaag tgctgccttc catctacaaa cagcatgaag
    11161 agcgctacgc cggttatacc ctgcgtcagt tgtgccagga aatgcatgat ttgtatgccc
    11221 gccacaacgt gaaacaactg caaaaagaga tgttccgcaa agcgcacttc ccacacgtca
    11281 gcatgaatcc gcaagaagcc aactacgcct atttacgcgg tgaagtggag ctggttcgtc
    11341 taccggatgc cgaaggtcgt atcgctgccg aaggggcgct tccttatcct ccgggtgtgc
    11401 tgtgtgttgt tccgggtgaa atctggggtg gtgctgtttt gcgttacttc agcgctctgg
    11461 aagaagggat caacctgctg ccaggttttg caccggagct gcagggtgtc tatatcgaag
    11521 aacatgatgg tcgtaagcaa gtttggtgct atgtcatcaa gcctcgtgat gcgcaaagca
    11581 ccctgttgaa aggggaaaaa ttatgagtca ggctaaatcg aacaaaatgg gcgtcgttca
    11641 gttaaccata ctgacgatgg tcaacatgat gggctccggt atcatcatgc tgccgacaaa
    11701 gcttgccgaa gttgggacaa tttcaattat ctcctggctg gtgacagccg taggctcaat
    11761 ggcactggca tgggcattcg caaaatgcgg tatgttcagc cgtaaatcag gtggtatggg
    11821 cggttatgcc gagtatgctt tcggtaaatc cggtaacttt attgcgaact atacctacgg
    11881 cgtctcactg ctgattgcta acgtcgcgat tgctatttcg gcggttggtt acggcaccga
    11941 actgctcggc gcgagtttgt cgccagtgca gattggtctt gcgaccatcg gggtgctgtg
    12001 gatttgtacc gtggctaact ttggtggtgc gcgcattacc gggcaaatca gtagcattac
    12061 cgtgtggggg ggcattattc cggtggtagg cctgtgcatt atcggctggt tctggtttag
    12121 cccgacgctt tacgttgatt cctggaatcc gcatcatgca ccgttcttca gtgcagtagg
    12181 ttcttccatc gctatgacgc tgtgggcttt tcttggtctg gagtctgcgt gtgcgaatac
    12241 tgatgtagtg gaaaacccgg aacgtaatgt gccaatcgcg gtactcggcg gtacgttagg
    12301 tgcggcggtg atttatatcg tctccaccaa cgtgattgca gggattgtgc ctaatatgga
    12361 gctggcaaat tcaacggcac catttggcct ggccttcgca cagatgttta caccagaagt
    12421 gggtaaagtc attatggcgc tgatggtgat gtcctgctgc ggttcgctgc ttggctggca
    12481 gttcaccatt gcccaggtgt ttaaatcttc atctgatgaa ggctacttcc ctaaaatttt
    12541 ctcccgtgta accaaagtgg atgcaccggt gcagggaatg ttgaccattg tgattatcca
    12601 gagcgggttg gcgctgatga cgattagccc gtcgctaaac agtcagttca acgtgctggt
    12661 taacctggcc gtggtgacca atatcattcc gtatattctg tcaatggcgg cattagtcat
    12721 tattcagaag gtcgctaatg tgcccccctc aaaagcgaaa gttgcaaact ttgttgcttt
    12781 tgttggcgcg atgtatagct tttatgcgct gtactcatcc ggggaagaag ccatgctgta
    12841 cggttccatc gtgaccttcc ttggttggac actgtatggt ctggtatcac cacgctttga
    12901 actgaaaaat aaacacggtt aaaaaagcaa agtaaaaaag ggcgatcttg cgaccgctct
    12961 ttttttatta aatgtgttta cgcgtttttc agaacttcgc taacaatctc aaccgcttct
    13021 ttctcaatct gcttgcgatg ttcttcaccg aggaagcttt cgcagtagat cttatatgcg
    13081 tcttccgtgc ctgacggacg cgcggcgaac cagccgttgt cagtcatcac tttcagaccg
    13141 ccaatagaag caccgttgcc cggagcagca gtcaggcgcg cggtgatcgg gtcacctgcc
    13201 agggtgctgg cgctcaccat ttccggagac agcttagaca gcgccgcttt ttgtgcggaa
    13261 gtcgcagctg cctgcaaacg gttgtagctc ggcgcaccaa agcgttttgc cagttcgttg
    13321 tagtgttcct gcgggttctt accggtgaca gcggtgattt ccgccgccag cagacacata
    13381 atgatgccgt ctttgtcggt ggaccacggc gtgccgtcga aacgcaggaa ggaagcccct
    13441 gcgctctctt cgccgccaaa gccgaagctg ccgtcgaaca gaccatcaac aaaccatttg
    13501 aaacctaccg gtacttctac cagcttacgg cccaagtcat tgaccacacg gtcgatcatc
    13561 gcagaagaaa ccagcgtttt accgacagca acatctttgc cccactgcgg acgatgctgg
    13621 aacaggtagt tgatcgccac cgccaggtag tggttcggat tcatcaaacc tgccggagtg
    13681 acgataccgt gacggtcata atccgggtcg ttagcaaacg ccagatcgaa cttatcacgc
    13741 agtgccagca gacccgccat cgcacactcg gaggagcagt ccatacggat cgcaccgtct
    13801 ttatcgaggt gcataaagcg gaaggtttga tcgacctgat cgttaacgat ggtcaggttg
    13861 aggttgtaat actcgccaat gcgcttccag tattcgatac cggaaccgcc caacggatca
    13921 acgcccagcg tcaggcccgc tttctgaatc gccgccatat caacgatatc ggccagccct
    13981 tccacgaacg gctgcaccag atcctgctct ttcacatagc cggatgccat cgcttcgtcg
    14041 agggagatac gcttcacgcc tttcaggcca tcggccatca gtgcgttggc cttgtcttcc
    14101 accactttgg tgacgttagt atcagccggg ccaccatttg gcggattgta cttgatacca
    14161 ccatcttccg gcgggttatg ggacggtgta atcacgatac cgtctgccag cgggccacct
    14221 tttttattgt gaaccaggat ggcattggaa acggcaggcg tcggagtgaa gccattgttt
    14281 tcctgcacaa tgacatcaac gccgttcgct gccagcactt ccagtacgga aatgaacgca
    14341 ggctcggaca gggcgtgagt atctttaccc acatagcaag ggccagtgat gccgtttttc
    14401 gcacgttctt cagcaattgc ctgagcgatc gccagaatgt gcggctcgtt aaagctgtgg
    14461 cgcgctgcac tgccacggtg accggaagta ccgaatttca ccgcgtgctc cgcattccct
    14521 gcttctggtt tcagtacata atattgcgcc gtcagttggg cgacgttaat caaatcactc
    14581 tgttgtgcag gttggcctgc acgattgtgg attgccattg ctttgtcctt tgtctgcaac
    14641 gttttagata gttccgcaaa ccttctcaat caattccgcc gggaattgca tcgactgcat
    14701 gatgtgttcg atcatgctgc atttacggcc ggtgttggtg ttggtgatca cccaatacgg
    14761 cgtgcctggc acatgtttcg gcttggtctg attaccattt ttcagcagcg tttgttcatc
    14821 tgccgcaaag tacacgcgtg tacgaccgtg caacgattcc gttgcttcgg caaacgcctg
    14881 ggcgtcaaga gaatatagtg tagacaacag cagcataaag cgattgaccg ctcgcttttg
    14941 ctctgcgtat tcgtccgaaa gcagaagttc acgcattgcg cgaaccttgt ctttaatcgt
    15001 tttgaccggc ttcgcttcga cgatagcagg tgacgcaacg cgaacctctt tcgtcaccgg
    15061 agcagcaggc tgtgatgcgg cggaaaattt caacatacgc cgtaaaatgt cggatgcgct
    15121 ctcgccgata tgcttagtgt ggctggcaat atagctgtag agttcatcat caacttcaat
    15181 cgttttcatc ttaatccagt gcagtgtctt agctgaatac aagtcgccaa gagtatatga
    15241 ataaatcccg gcagcggata gcgtcaagcc tggcttgcag aaaaactcgg aataaacctt
    15301 atttgttgca gccctgtggc acaatggtca acataatacc ctaacccgac aacgcgaaaa
    15361 aaagaacttt gccatgaaat taaatatccg cgcgcaaact gcacaaaacc agcacaataa
    15421 ttctcccatc gttcttgtcc atggtctgtt tggtagcctc gacaaccttg gcgtactggc
    15481 tcgcgatctg gtaaacgatc acaatatcat ccaggttgat atgcgtaacc acggtctttc
    15541 accgagagag ccggtaatga attacccggc gatggcgcag gatcttgttg ataccctgga
    15601 tgcactgcag atcgacaaag caacatttat cggtcactcc atgggcggta aagcggtaat
    15661 ggcacttact gcactggccc ccgatcgcat cgataaactg gtggcgatcg atatcgcacc
    15721 ggtcgactat cacgtacgcc gtcatgatga gattttcgca gctatcaacg cggtcagtga
    15781 atcggacgca caaactcgcc agcaagcagc agcaataatg cgccagcatc ttaatgaaga
    15841 aggggtgatt cagtttctgt tgaaatcttt tgttgacggg gagtggcgct ttaacgtgcc
    15901 ggtattgtgg gatcagtatc cgcatattgt gggttgggag aaaatcccgg catgggatca
    15961 ccctgccctg tttatccctg gcggcaattc tccgtatgtt agcgagcagt accgtgatga
    16021 tttactggca caatttccac aggcacgggc gcatgtgatt gcaggcgcgg gtcactgggt
    16081 ccatgctgaa aaaccggatg cggtattacg cgctatccgt cgctatctca atgattaacg
    16141 caactgatta aaaacccagc gtccgcgtcg ctttccgcgc gtgggcgttg gcgtgactca
    16201 tcgcgctgat gtattatggc gcgctatcca tccgggcaga ggcctggctg attgtttccc
    16261 ccgaagtcac caagatcatg gccaaagaac aaacggaccg tacgacatta gatctgttcg
    16321 cgcacgagcg tcgaccggga cgaccgaaaa ctaatccgct ttcgcgcgat gaacagctgc
    16381 gtattaataa acgcaaccag ctaaaacgcg acaaagtacg tggccttaag cgtgtcgaac
    16441 tgaagctgaa cgcggaagct gtcgaggcgc tgaacgagct ggcggaatcg cgtaatatga
    16501 gccgtagcga actgatcgaa gagatgttaa tgcagcaact ggcggcgctg cgtagtcagg
    16561 gcatcgttta aatttccact ttcatgtagc acagtgtgca gtcctgctcg tttgctgatt
    16621 ccgcaagact gcctgttctg ctatgattgc ctttatccgt gggcaatttt ccacccccat
    16681 ttcaataagt ttcaagaggt tatttcactc atggctatca ctggcatctt tttcggcagc
    16741 gacaccggta ataccgaaaa tatcgcaaaa atgattcaaa aacagcttgg taaagacgtt
    16801 gccgatgtcc atgacattgc aaaaagcagc aaagaagatc tggaagctta cgacattctg
    16861 ctgctgggca tcccaacctg gtattacggc gaagcgcagt gtgactggga tgacttcttc
    16921 ccgactctcg aagagattga tttcaatggt aaactggttg cgctgtttgg ttgtggtgac
    16981 caggaagatt acgccgaata tttctgcgac gcattgggca ccatccgcga catcattgaa
    17041 ccgcgcggtg caaccatcgt tggtcactgg ccaactgcgg gctatcattt cgaagcatca
    17101 aaaggtctgg cagatgacga ccactttgtc ggtctggcta tcgacgaaga ccgtcagccg
    17161 gaactgaccg ctgaacgtgt agaaaaatgg gttaaacaga tttctgaaga gttgcatctc
    17221 gacgaaattc ttaatgcctg atgtgatgcg gcgtagactc atgtctacgc cgtattaata
    17281 gataatgcca atcaaaataa ttgctacaaa tttgtaactt ttgctgttgt acctatacaa
    17341 tgtcccagtg ttcaagtggc cttgccgttg taaatgtaag ctgtgccacg tttttattaa
    17401 caatatttgc cagggacttg tggttttcat ttaggcgtgg caattctata atgatacgca
    17461 ttatctcaag agcaaattct gtcacttctt ctaatgaagt gaaccgctta gtaacaggac
    17521 agattccgca tgactgataa caataccgcc ctaaagaaag ctggcctgaa agtaacgctt
    17581 cctcgtttaa aaatcctgga agttcttcag gagccggaca accatcacgt cagtgcggaa
    17641 gatttataca aacgtctgat cgatatgggt gaagaaattg gtctggctac ggtatatcgc
    17701 gtactgaacc agtttgacga cgctggtatc gtcacccgcc acaattttga aggcggtaaa
    17761 tccgtatttg aactgacaca gcaacatcac cacgatcacc tgatttgcct cgactgcggc
    17821 aaggttatcg aatttagtga tgattccatc gaagcgcgtc agcgtgaaat tgccgcaaaa
    17881 catggcattc gcctgactaa ccacagtctc tatctttacg gtcactgtgc cgaaggcgat
    17941 tgccgcgaag atgagcacgc gcacgaaggc aaataagcca gcctgaacga gaaaagccaa
    18001 cctgcgggtt ggctttttta tacaagggaa ataagaaaag cgagagttac agctctcact
    18061 tatttgttat tactgcgtat ttcctgccag accttatcac aatcggcttt caccgcctga
    18121 tcatttccgg tttgcgttgc acgcagacac tgctgataat ccagtacgcg tacactttcc
    18181 tggtcggcaa actgctgatg ttgtttctct ttcttcagca cgtttaacac gctctggcag
    18241 gcttcaattt tttctggcga accttgcgcg gtgttgatac aggcgctgta tgcctctttc
    18301 agacggctgt cttcctgtgg tgcggttgat tgcgcacaag ctaccagccc cgatgccatc
    18361 atggcgatga gaatcagttt tttcattgaa tgcggcaggc acaaaacctg ccgcgtcggg
    18421 catcagaaga tggtgaatgg tgcgattacc ataaatttca cgtcacgctc atcctggaag
    18481 atgttaccgt aaccgccgcc ccagcttggg atgtcggagt ggttgtcgta ttcggtgaag
    18541 tgcagtttga acatcgtgcc tttggcgcga ccgtcctgaa tggtgtagac cgcatccagg
    18601 ctgtaggcag actcttcaat agtccggttt ttgtcgtagt acgcatccgg attgctctgc
    18661 caggtcgcag gtttagcatc ccatgcgtaa acgtaggaag cgccgatggc gaagcctgga
    18721 agattccagt ttttcaggtc atacatcgca ctgaagaaga ccgctttttc gccgttagcg
    18781 ttgaagtcgg aacggttatc ccaccagata tccaggcggc cgttggagga agcgtaggtt
    18841 ggggtcatac gttgcaggaa gtatccctgc tgactgtcag ccttaatcca ggtgccttcg
    18901 aggcgcaaat ccactacgtc agccgcccgg taaccaaagg tcaacgcttg cagccaggcg
    18961 gtgccgtcat aaagatcgtt gacgctgcga tcgtcaactt tatcgcgggt accatagaac
    19021 tggtagctgg tggttaacgg gctaccggcg atatcaaatt tgtagctggc tttggcaaaa
    19081 tattgatcga tatacccttc cgcctgacca aatgccgctt ccagtacgaa gttatttttg
    19141 aagtcgtatt tcgcaccgat ggagtgcaga taatcaactt tggtggtttt atcgttctga
    19201 taaaactcat ccatttccag atgccacggc gctttgtatt cgttggtcca catgtaggag
    19261 aaactcaagg caccagcatc gccgtaatca aaattcgccc cggcttccgc cccctgataa
    19321 gtacccggca taaagctcca gtgcggcgct aacagcgttt gaccggttgg ctgaatgtaa
    19381 cctgccctcg cccaaaccgg accgtattta aatttggccg cagctttata caggcttata
    19441 ccgcttttgt cgccggacca gtcttcgtca taggctttat tactttttga aaacgcgatt
    19501 tcgttcgggt gggagctgtc gccgttttcc gccatttcaa tcgccgtaaa cgcagcaata
    19561 tcaaggccga acatatcagc agcataaccg gactgaaaat cgaggttggc attccaggtg
    19621 gagtgagaaa ggttggtttt gtatttgtcg ccgtcggtaa catctttacg gtcgcgttca
    19681 cgctgccagt aatagatacc gccggttaag gttgaatcgt cgatgaatcc ttcggcgcga
    19741 gcctccggca ttgccataaa gcccgacatt gctgtaacac cggcaatagc cagcgccagc
    19801 gtactacgtt tgccactaaa cgtacgcatg ggttaatcct ctttgacgta taaattgctg
    19861 caccaaaggt gaacagcgaa aaaaaataaa aattaaaaaa gtagcgttga gaaaagtctc
    19921 gctgactaca ggaacagact atttttcgcg acgcgaatta tcaatctctt tctgtaacca
    19981 gaatgtaaga acatgagcaa ccgcacatat tttgatgatt tttgttacaa ggcttaagtt
    20041 tgcggttgaa aggcttgcag aagaataaaa acgtgaaatt ccagcaagat gaaagcggtt
    20101 acattttatt taattatgtt ttgtgatttt aaatttattt tatggataaa ttgcgaagcg
    20161 gaattaattc gcgaagaaaa gcagaaaaaa cgccgcagag cgcggcgttt gggattgttt
    20221 gctatttctt gccggatgcg gcgtgaacgc cttatccggc ctacaaaagc acgcaaattc
    20281 aatatattgc agagatcatg taggcctgat aagcgaagcg catcaggcaa tttagcgttt
    20341 gaaatttatt gtctatttat tgccggatgc gacgtaaatg cctcacccgg cttggtagaa
    20401 cttaaaatta ctcgcctact ttcgcccagg tatcacgcag cccaacggtg cggttaaata
    20461 ccggtttttc ggccgtggaa tggcggctgt cgaggcagaa gtaaccttca cgctcaaact
    20521 ggaatgcttt acccgcaacc gcatctttca gcgacggttc agcaaagccc tgtttgatca
    20581 ccagcgattc cgggttaatc accgacagga aatcatccgc agcacctggg ttaggcacgc
    20641 tgaacagacg atcatacaaa cggatttcaa ccggcagcgc atgtgccgcg ctcacccagt
    20701 gaataacacc tttgacttta cgaccatctg ccggatcttt gcttaaggtg tcggcgtcat
    20761 aagtacagaa gatggtggtg atattacctt cggcatcttt ctccacgcgt tcagccttaa
    20821 tgacgtaagc attacgcaga cgcacttctt tacccagcac cagacgtttg tactgcttgt
    20881 tagcttcttc gcggaaatcg gcgcgatcga tccaaatttc accgctaaac ggcacctgac
    20941 ggctccccat ttccggtttg ttcggatggt tcggcatggt gaccatttcg ccttcgccct
    21001 gatagttttc gataaccagt ttcaccggat cgataaccgc cattgcgcgc ggcgcatttt
    21061 cgttgagatc ttcacggatg caggattcca gcgacgccat ctcaatggtg ttgtcctgct
    21121 tggtcacgcc gatgcgtttg cagaactcac gaatagaagc cgcagtgtaa ccacgacgac
    21181 gcagaccgga aatggtcggc atacgcgggt catcccagcc ttcaacgtgc ttgtcggtca
    21241 ccagcaggtt cagcttacgc ttggacatca cagtgtattc cagattcagg cgcgagaatt
    21301 cgtactggcg cggatgaaca ggaatggtga tgttatccag tacccagtcg tacagacgac
    21361 ggttgtcctg gaactcaagc gtacacagag agtgcgtaat accttccagc gcatcgctga
    21421 tgcaatgggt gaagtcgtac atcgggtaga tgcaccactt gttgccagtc tggtggtgtt
    21481 cagcaaattt aatgcggtac agcaccggat cgcgcattac gataaatggc gacgccatgt
    21541 cgattttcgc acgcaggcag gctttacctt cttcaaaacc accggcacgc attttttcga
    21601 acagcgccaa gttctcttca acgctgcggt cgcggtacgg gctgttttta cccggctgcg
    21661 tcagggtacc gcggtattcg cggatctgtt ccggtgtcag ttcatcaacg tacgccaggc
    21721 ctttattgat cagttcgatc gcataggcgt ggagctgatc aaaataatcg gaggagtaac
    21781 ggacgttacc agaccagtga aaacctaacc actctacgtc gtttttgatc gaatcgacat
    21841 actcgatatc ttcttttacc gggttagtgt cgtcgaaacg caggttgcac tggcctttat
    21901 agtcctgggc gatcccgaag ttcaggcaga tagatttcgc atggccaata tgcagatagc
    21961 cattcggctc cggcgggaaa cgggtgtgta ctgtgatgtg cttaccactg gccagatctt
    22021 catcgatgat ctgacggata aagttagtcg ggcgggcttc tgcctcactc atcgtggatt
    22081 cctcaaagcg taaacaacgt ataacggcgt atgatcttat aagcgggaca ggctgacaac
    22141 tgttagtttt ttaaaattga taatctaaca aggattatgg ggatgattga tgaaaaaaaa
    22201 agcggcagag aagatctcca ccgcttgtca ttgttggatg cgacgctgcc gtgtcgcatc
    22261 aggcataaag cagattactt tttgatttca tacagcggtg tttgacccgc cacaacatgg
    22321 ccctgagctt taatgagcaa gccactgaaa tcgtcgatat tgctgcaaac caccgggcta
    22381 atcatcgagc gggcgttagc gttcaggtaa tccagatcca tttccagaat cggttgccct
    22441 gcgcttacct gcgcaccctc ttccaccaga cgtttaaagc ctttaccttc cagcgctacg
    22501 gtgtcgatac ccatatggac gacgatctcc gcgccttttt cggtttccag gcagaacgcg
    22561 tggttggtgt tgaagatttt cacgattgtc cctgcggctg gtgatacgac gattttatct
    22621 gtcggtttca ccgccacacc gtcacccacc gctttgctgg cgaatgcttc gtcaggaacc
    22681 tgatccagtg ccacgacatc accggtaatc ggcgatacca gctccgcgat agataccgcg
    22741 tttggtacag cctgcggttt tgctacaggc gcggcagttg ccggagttgc ttcagctgac
    22801 gcagcggcta ccggaccacg ggcaacgact ttcttcatcg catcgccgat ggattctgct
    22861 ttcgcgccaa caatcacctg aatagtctgt ttgttcagtt tcactacacc agaagcaccc
    22921 agacgtttac acatcgtatc gttgacgcgg gcagagtcag ccacggtcag gcgcagacgg
    22981 gtaatacagg catcaatcgc tttcaggttg tcagtaccgc caaccgcagc aatatagttg
    23041 gttgccagtt gagtcagacc ttcttcagta ttgctgttgg cttcttcagt aacgatctcg
    23101 tcttctttat cttcacgacc cggcgttttc aggttgaaca tgcggataac caaactgaac
    23161 accacgaagt agatagcgaa gaagataacg cccatcacca gcagcatcca gacgttctgg
    23221 ctggcggcag gcaggttata catcaacgcg tagtcgatag cacccgcaga gaaagagaag
    23281 ccggcatgga tacccagcag cgttgccaca aacaggctga taccggtcag cagtgcgtgc
    23341 aggaggtaca gcagcggcgc aaggaacatg aacaggaatt ccagcggctc agtcacaccg
    23401 gtcaggaacg cagtaacagc aacagaaagc agcatcccgc caaccatcgg acgacgctct
    23461 ttcggtgctg cgaagtacat cgccagcgcc gcacccggca gaccgaacat catgatcggg
    23521 aagaagccgg acatgaacat ccccgcggtg ccgtcaccag cgtagaaacg gttgatgtcg
    23581 ccgtggaaaa ccgtacccgc cgcgttggtg aattcaccaa tctggaacca ggcgatggtg
    23641 ttcagcacct gatgcagacc ggttgggatc agcagacggt tgatgaaacc aaagataccg
    23701 gaacccagcg cgcccgcaga aacgatccac tcgccgcctg catggatagc gtgctgtacc
    23761 ggcggccaga cgtaaccaaa aatggccgcc agcaccagac agaagaagcc ggtggcgatc
    23821 ggcacaaagc gtttgccgcc gaagaagctc aggaagtccg gcagtttaat atcggaccaa
    23881 cggttatagg ctgcgccacc aaccagaccg gtaatgatac ccgccagtac acccatgtta
    23941 atttctgggt tgatggtcac catcgctttg gttaacacaa agtaacctac cgcacccgcc
    24001 agcgccgccg cacctgcgct gtctttcgac cagctggatg ccacaccgat ggcgaagatt
    24061 aatgcgaggt tatcaaaaat tgcaccgccc gcctgggcaa taaacgcaac gttaagtaaa
    24121 tctggctgac cgaatcgcag caacagcgcc gccaccggca gcaccgcgat agggagctgt
    24181 aacgccctac cgagtcgctg gaaaaaacct aaaatattca tcttattccc cctacgagaa
    24241 ccctatttgg ctcgtttaaa gccgtatttt tattttgctg caaattttac tgccgatgtt
    24301 ctgtaatcag attgttagat catctgctac agagtgtgtg aaaatttaat tcgcatcgca
    24361 aattaaacgc gtgtcttttg tgagttttgt caccaaatat cgttatcatc actccctttt
    24421 actggctaaa ccagaaaact tattttatca ttcaaaaaat caagtcggat tgacgcctgt
    24481 ctgcgcaaat ccaggttacg cttaaagatg tctaatccgc caacggctta cattttactt
    24541 attgaggtga ataatgagac tgatccccct gactaccgct gaacaggtcg gcaaatgggc
    24601 tgctcgccat atcgtcaatc gtatcaatgc gttcaaacca actgccgatc gtccgtttgt
    24661 actgggcctg ccgactggcg gcacgccgat gaccacctat aaagcgttag tcgaaatgca
    24721 taaagcaggc caggtcagct ttaagcacgt tgtcaccttc aacatggacg aatatgtcgg
    24781 tctgccgaaa gagcatccgg aaagctacta cagctttatg caccgtaatt tcttcgatca
    24841 cgttgatatt ccagcagaaa acatcaacct tctcaacggc aatgccccgg atatcgatgc
    24901 cgagtgccgc cagtatgaag aaaaaatccg ttcttacgga aaaattcatc tgtttatggg
    24961 cggtgtaggt aacgacggtc atattgcatt taacgaaccg gcgtcttctc tggcttctcg
    25021 tactcgtatc aaaaccctga ctcatgacac tcgcgtcgcg aactctcgtt tctttgataa
    25081 cgatgtcaat caggtgccaa aatatgccct gactgtcggt gttggtacac tgctggatgc
    25141 cgaagaagtg atgattatgg tgctgggtag ccagaaagca ctggcgctgc aggccgccgt
    25201 tgaaggttgc gtgaaccata tgtggaccat cagctgtctg caactgcatc cgaaagcgat
    25261 catggtgtgc gatgaacctt ccaccatgga gctgaaagtt aagactttaa gatatttcaa
    25321 tgaattagaa gcagaaaata tcaaaggtct gtaattgtta tccctgccct ggttccttgc
    25381 tcagggcaat attttttaaa atcggggggc agaatgtatg cattaaccca gggccggatc
    25441 tttaccggcc acgaatttct tgatgaccac gcggttgtta tcgctgatgg cctgattaaa
    25501 agcgtctgtc cggtagcgga actgccgcca gagatcgaac aacgttcact gaacggggcc
    25561 attctctccc ccggttttat cgatgtgcag ttaaacggct gcggcggcgt acagtttaac
    25621 gacaccgctg aagcggtcag cgtggaaacg ctggaaatca tgcagaaagc caatgagaaa
    25681 tcaggctgta ctaactatct gccgacgctt atcaccacca gcgatgagct gatgaaacag
    25741 ggcgtgcgcg ttatgcgtga gtacctggca aaacatccga atcaggcgtt aggtctgcat
    25801 ctggaaggtc cgtggctgaa tctggtaaaa aaaggcaccc ataatccgaa ttttgtgcgt
    25861 aagcctgatg ccgcgctggt cgatttcctg tgtgaaaacg ccgacgtcat taccaaagtg
    25921 accctggcac cggaaatggt tcctgcggaa gtcatcagca aactggcaaa tgccgggatt
    25981 gtggtttctg ccggtcactc caacgcgacg ttgaaagaag caaaagccgg tttccgcgcg
    26041 gggattacct ttgccaccca tctgtacaac gcgatgccgt atattaccgg tcgtgaaccg
    26101 ggcctggcgg gcgcgatcct cgacgaagct gacatttatt gcggtattat cgctgatggc
    26161 ctgcatgttg attacgccaa cattcgtaac gctaaacgcc ttaaaggcga caaactgtgt
    26221 ctggttaccg acgccaccgc gccagcaggt gccaacattg aacagttcat ttttgcgggt
    26281 aaaacaatat actaccgtaa cggactttgt gtggatgaga acggtacgtt aagcggttca
    26341 tccttaacca tgattgaagg cgtgcgtaat ctggtcgaac attgcggtat cgcactggat
    26401 gaagtgctgc gtatggcgac gctctatccg gcgcgtgcga ttgacgttga aaaacgtctc
    26461 ggcacgctcg ccgcaggtaa agtagccaac ctgaccgcat tcacacctga ttttaaaatc
    26521 accaagacca tcgttaacgg taacgaggtc gtaactcaat aagagaaagt atgacaccag
    26581 gcggacaagc tcagataggt aatgttgatc tcgtaaaaca gcttaacagc gcggcggttt
    26641 atcgcctgat tgaccagtac gggccaatct cgcggattca gattgccgag caaagccagc
    26701 ttgcccccgc cagcgtaacc aaaattacgc gtcagcttat cgaacgcggg ctgatcaaag
    26761 aagttgatca gcaggcctcc accgggggcc gccgcgctat ctccatcgtc accgaaaccc
    26821 gcaatttcca cgcaatcggc gtacggcttg gtcgtcatga cgccaccatc actctgtttg
    26881 atctcagcag caaagtgctg gcagaagaac attacccgct gccggaacgt acccagcaga
    26941 cgctggaaca tgccctgctg aatgccattg ctcagtttat tgatagctac cagcgcaagc
    27001 tgcgcgagct aatcgccatt tcggtgatcc tgccagggct tgttgacccg gacagcggca
    27061 aaattcatta catgccgcat attcaggtag aaaactgggg gctggtagaa gcactggaag
    27121 aacgttttaa agtgacctgt ttcgttggtc acgatatccg tagtctggcg ctggcggagc
    27181 actacttcgg tgcaagtcag gattgcgaag actccattct ggtgcgtgtc catcgcggaa
    27241 ccggggccgg gattatctct aacgggcgca tttttattgg ccgcaacggt aacgtcggtg
    27301 aaattggcca tattcaggtc gaaccgctgg gtgaacgctg ccactgcggc aactttggct
    27361 gcctggaaac tatcgccgcc aacgctgcca ttgaacaacg ggtgttgaat ctgttaaagc
    27421 agggctacca gagccgtgtg ccactggacg actgcaccat caaaactatc tgcaaagccg
    27481 cgaacaaagg cgatagcctg gcctcggaag tgattgagta tgtcggtcgt catctgggta
    27541 aaaccatcgc cattgctatc aacttattta atccgcaaaa aattgttatt gctggtgaaa
    27601 tcaccgaagc cgataaagtg ctgctccctg ctattgaaag ctgcattaat acccaagcgc
    27661 tgaaggcgtt tcgtactaat ctgccggtgg tacgttctga gctggatcac cgctcggcaa
    27721 tcggcgcttt tgcgctggta aaacgcgcca tgctcaacgg tattttgctc cagcatttgc
    27781 tggaaaatta atgtgctttt atagtggcgc ttattgttgt caatattctg ggtagtccat
    27841 gaccattaaa aatgtaattt gcgatatcga cggcgtgctg atgcacgata acgtcgccgt
    27901 accgggtgca gcggaatttt tgcacgggat tatggataaa ggcctgccgc tggtgttgct
    27961 gaccaactat ccttcgcaga ctgggcaaga tctggcgaac cgctttgcca ctgccggtgt
    28021 cgatgtgccg gatagcgtgt tttatacctc tgcgatggcg accgccgatt tcctgcgtcg
    28081 ctaggaaggc aagaaagcgt atgtggtggg cgaaggcgca ctgattcatg aactgtacaa
    28141 agccggtttc actattaccg atgtgaaccc tgatttcgtg attgttggcg aaacacgttc
    28201 ctacaactgg gacatgattc ataaagcagc ctatttcgtc gctaacggtg cacgctttat
    28261 cgccaccaac ccggacaccc acgggcgcgg tttttatccc gcttgtggcg cgttgtgtgc
    28321 agggattgag aaaatctccg ggcgcaaacc gttctatgtt ggtaagccca gcccgtggat
    28381 catccgcgcg gcattaaaca aaatgcaggc gcattcggaa gaaacagtga ttgtcggcga
    28441 taacctgcgt accgatattc tggcgggctt ccaggccggt ctggagacga ttctggtgct
    28501 ttctggtgtt tcatcgctcg acgatatcga cagtatgccg ttccgcccaa gctggattta
    28561 cccgtcagtc gctgaaatcg acgttatctg aataccaccc acgtctgcgg acgtggttgg
    28621 ctctgatgac aaacgccaaa ctgcctgatg cgctacgctt atcaggccta cgcagctcct
    28681 gcaatatatt gaattttcat gcttttgtag gccggataag gcgttcacgc cgcatccggc
    28741 atgaacaaat cgcactttat cagcaatttg gcccccgtaa aatataacca ttcaataaaa
    28801 cccgctaaaa attatcgatt catcaataaa tacatcctca caccagaccc tttttcacca
    28861 atcgacaatc accattacgt tttttatttt tgcactgcca aatagcttgc gcacagtgct
    28921 gctttgcggc atttttttaa acaagcaaac acaacaagca acaaatacca ggttaacgga
    28981 gaaggttatg tgttcaattt ttggcgtatt cgatatcaaa acagacgcag ttgagctgcg
    29041 taagaaagca ctcgagctgt cacgcctgat gcgtcatcgt ggcccggact ggtccggtat
    29101 ttatgccagc gataacgcca ttctcgctca cgaacgtctg tcaattgttg acgttaacgc
    29161 gggggcgcaa cctctctaca accaacaaaa aaaccacgta ctggcggtaa acggtgaaat
    29221 ctacaaccac caggcattgc gcgctgaata tggcgatcgt tatcagttcc agacagggtc
    29281 tgactgtgaa gtgatcctcg cgctgtatca ggaaaaaggg ccggaatttc tcgacgactt
    29341 gcagggcatg tttgcctttg ccctgtacga cagcgaaaaa gatgcctacc tgattggtcg
    29401 cgaccatctg gggatcatcc cactgtatat ggggtatgac gaacacggtc agctgtatgt
    29461 ggcctcagaa atgaaatcgc tggtgccagt ttgccgcacg attaaagagt tcccggcggg
    29521 gagctatttg tggagtcagg acggcgaaat ccgttcttat tatcatcgcg actggttcga
    29581 ctttgatgca gtgaaagata acgtgaccga caaaaacgag ctgcgtcagg cactggaaga
    29641 ttccgttaaa agccatctga tgtctgatgt gccttacggt gtgctacttt ctggtggtct
    29701 ggattcctca attatttccg ctatcaccaa gaaatacgca gcccgtcgcg tggaagatca
    29761 ggaacgctct gaagcctggt ggccgcagtt acactccttt gctgtaggtc tgccgggttc
    29821 accggatctt aaagcagccc aggaagtggc aaaccatctg ggcacggtgc atcacgaaat
    29881 tcacttcact gtacaggaag gtctggatgc catccgcgac gtgatttacc acatcgaaac
    29941 ttatgatgtg accactattc gcgcttcaac accgatgtat ttaatgtcgc gtaagatcaa
    30001 ggcgatgggc attaaaatgg tgctgtccgg tgaaggttct gatgaagtgt ttggcggtta
    30061 tctttacttc cataaagcgc ccaacgttaa agaactgcat gaagagacgg tgcgtaaact
    30121 gctggccttg catatgtatg actgcgcgcg cgccaacaaa gcgatgtcag cctggggcgt
    30181 ggaagcacgc gttccgttcc tcgacaaaaa attccttgat gtggcgatgc gcattaaccc
    30241 gcaggataaa atgtgcggta acggcaaaat ggaaaaacac atcctgcgtg aatgttttga
    30301 gtcatacctg cccgcaagcg tggcctggcg gcagaaagag cagttctccg atggcgtcgg
    30361 ttacagttgg atcgacaccc tgaaagaagt ggcggcgcag caggtttctg atcagcaact
    30421 ggaaactgcc cgcttccgct tcccgtacaa cacgccgacc tcaaaagaag cgtatctgta
    30481 ccgcgagatc tttgaagaac tgttcccact tccgagtgcc gctgagtgcg tgccgggcgg
    30541 tccttccgtc gcgtgttctt cagctaaagc gatcgaatgg gatgaagcgt tcaaaaaaat
    30601 ggacgatccg tctggtcgtg cggttggcgt tcaccagtcg gcgtataagt aagaaatgcg
    30661 gtactgcccc ggagaaatcc ggggctgttt tagttattgc gctgcttgtt gattctgata
    30721 tgcagtaagg aaatgttccg ggtttgccac atcactgttt gacaactcat cactgtaggc
    30781 cagtatttca tagattaatt tcccaccact attgttgaat tccgaatttc atcctgtacg
    30841 acaaaagaat ggctgtaggg tggggaaaaa cacaaagtaa acaataattg acgaatatag
    30901 cgccacgctg ttcgcaacct aaccaaacag tcactttcga gcaattttcc ttgaaaaaga
    30961 ggttgacgct gcaaggctct atacgcataa tgcgccccgc aacgccgata aggtatcgcg
    31021 aaaaaaag
//
LOCUS       DADLTY010000029        30372 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.029, whole genome shotgun sequence.
ACCESSION   DADLTY010000029 DADLTY010000000
VERSION     DADLTY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.029"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..378
                     /locus_tag="JN334_001114"
     CDS             <1..378
                     /locus_tag="JN334_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7437334.1"
                     /translation="RAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILS
                     KDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDD
                     GLRFAIREGGRTVGAGVVAKVLS"
     gene            608..991
                     /gene="secE"
                     /locus_tag="JN334_001115"
     CDS             608..991
                     /gene="secE"
                     /locus_tag="JN334_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAY7437335.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            993..1538
                     /gene="nusG"
                     /locus_tag="JN334_001116"
     CDS             993..1538
                     /gene="nusG"
                     /locus_tag="JN334_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAY7437336.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1697..2125
                     /gene="rplK"
                     /locus_tag="JN334_001117"
     CDS             1697..2125
                     /gene="rplK"
                     /locus_tag="JN334_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAY7437337.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2129..2833
                     /gene="rplA"
                     /locus_tag="JN334_001118"
     CDS             2129..2833
                     /gene="rplA"
                     /locus_tag="JN334_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAY7437338.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3246..3743
                     /gene="rplJ"
                     /locus_tag="JN334_001119"
     CDS             3246..3743
                     /gene="rplJ"
                     /locus_tag="JN334_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAY7437339.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3810..4175
                     /gene="rplL"
                     /locus_tag="JN334_001120"
     CDS             3810..4175
                     /gene="rplL"
                     /locus_tag="JN334_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAY7437340.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4495..8523
                     /gene="rpoB"
                     /locus_tag="JN334_001121"
     CDS             4495..8523
                     /gene="rpoB"
                     /locus_tag="JN334_001121"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAY7437341.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8600..12823
                     /gene="rpoC"
                     /locus_tag="JN334_001122"
     CDS             8600..12823
                     /gene="rpoC"
                     /locus_tag="JN334_001122"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAY7437342.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13065..14198)
                     /gene="thiH"
                     /locus_tag="JN334_001123"
     CDS             complement(13065..14198)
                     /gene="thiH"
                     /locus_tag="JN334_001123"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAY7437343.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14195..15040)
                     /gene="thiG"
                     /locus_tag="JN334_001124"
     CDS             complement(14195..15040)
                     /gene="thiG"
                     /locus_tag="JN334_001124"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAY7437344.1"
                     /translation="MSSGRNISCRMATRSCFFRLLQGVEMLRIADKTFDSHLFTGTGK
                     FASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIAAGVALLPNTSGAKTAEE
                     AIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLVQQGFVVLPYCGADPV
                     LCKRLEEVGCAAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHATQA
                     LEMGADAVLVNTAIAVADDPVNMAKAFRLAVEVGLLARQSGPGSRSYFAHATSPLTGF
                     LEASA"
     gene            complement(14967..15167)
                     /gene="thiS"
                     /locus_tag="JN334_001125"
     CDS             complement(14967..15167)
                     /gene="thiS"
                     /locus_tag="JN334_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAY7437345.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15151..15906)
                     /gene="thiF"
                     /locus_tag="JN334_001126"
     CDS             complement(15151..15906)
                     /gene="thiF"
                     /locus_tag="JN334_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HAY7437346.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15899..16534)
                     /gene="thiE"
                     /locus_tag="JN334_001127"
     CDS             complement(15899..16534)
                     /gene="thiE"
                     /locus_tag="JN334_001127"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY7437347.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16534..18429)
                     /gene="thiC"
                     /locus_tag="JN334_001128"
     CDS             complement(16534..18429)
                     /gene="thiC"
                     /locus_tag="JN334_001128"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAY7437348.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18510..18610)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18663..19139)
                     /gene="rsd"
                     /locus_tag="JN334_001129"
     CDS             complement(18663..19139)
                     /gene="rsd"
                     /locus_tag="JN334_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HAY7437349.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19234..20007
                     /gene="nudC"
                     /locus_tag="JN334_001130"
     CDS             19234..20007
                     /gene="nudC"
                     /locus_tag="JN334_001130"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HAY7437350.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20047..21111
                     /gene="hemE"
                     /locus_tag="JN334_001131"
     CDS             20047..21111
                     /gene="hemE"
                     /locus_tag="JN334_001131"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAY7437351.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21268..21939
                     /gene="nfi"
                     /locus_tag="JN334_001132"
     CDS             21268..21939
                     /gene="nfi"
                     /locus_tag="JN334_001132"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HAY7437352.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21982..22572
                     /locus_tag="JN334_001133"
     CDS             21982..22572
                     /locus_tag="JN334_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HAY7437353.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22759..23031
                     /gene="hupA"
                     /locus_tag="JN334_001134"
     CDS             22759..23031
                     /gene="hupA"
                     /locus_tag="JN334_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HAY7437354.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23044..23739
                     /locus_tag="JN334_001135"
     CDS             23044..23739
                     /locus_tag="JN334_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAY7437355.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23741..24166)
                     /gene="zraP"
                     /locus_tag="JN334_001136"
     CDS             complement(23741..24166)
                     /gene="zraP"
                     /locus_tag="JN334_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HAY7437356.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24404..25780
                     /gene="zraS"
                     /locus_tag="JN334_001137"
     CDS             24404..25780
                     /gene="zraS"
                     /locus_tag="JN334_001137"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HAY7437357.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25777..27102
                     /gene="zraR"
                     /locus_tag="JN334_001138"
     CDS             25777..27102
                     /gene="zraR"
                     /locus_tag="JN334_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HAY7437358.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27099..28388)
                     /gene="purD"
                     /locus_tag="JN334_001139"
     CDS             complement(27099..28388)
                     /gene="purD"
                     /locus_tag="JN334_001139"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAY7437359.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28400..29989)
                     /gene="purH"
                     /locus_tag="JN334_001140"
     CDS             complement(28400..29989)
                     /gene="purH"
                     /locus_tag="JN334_001140"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAY7437360.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7111 a         7914 c         8359 g         6988 t
ORIGIN      
        1 cgtgctggtg agaacgtagg tgttctgctg cgtggtatca aacgtgaaga aatcgaacgt
       61 ggtcaggtac tggctaagcc gggcaccatc aagccgcaca ccaagttcga atctgaagtg
      121 tacattctgt ccaaagatga aggcggtcgt catactccgt tcttcaaagg ctaccgtccg
      181 cagttctact tccgtactac tgacgtgact ggtaccatcg aactgccgga aggcgtagag
      241 atggtaatgc cgggcgacaa catcaaaatg gttgttaccc tgatccaccc gatcgcgatg
      301 gacgacggtc tgcgtttcgc aatccgtgaa ggcggccgta ccgttggcgc gggcgttgta
      361 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
      421 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      481 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      541 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      601 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      661 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      721 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      781 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      841 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      901 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      961 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
     1021 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
     1081 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
     1141 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
     1201 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
     1261 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
     1321 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1381 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1441 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1501 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1561 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1621 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1681 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1741 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1801 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1861 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1921 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1981 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     2041 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     2101 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     2161 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     2221 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     2281 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     2341 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2401 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2461 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2521 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2581 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2641 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2701 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2761 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2821 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2881 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2941 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     3001 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcttggcc tatccaggcc
     3061 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     3121 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     3181 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     3241 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     3301 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3361 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3421 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3481 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3541 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3601 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3661 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3721 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3781 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3841 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3901 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3961 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     4021 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     4081 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     4141 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     4201 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     4261 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     4321 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4381 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4441 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4501 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4561 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4621 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4681 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4741 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4801 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4861 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4921 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4981 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     5041 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     5101 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     5161 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     5221 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     5281 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     5341 aaactgattg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5401 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5461 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5521 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5581 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5641 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5701 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5761 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5821 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5881 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5941 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     6001 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     6061 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     6121 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     6181 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     6241 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     6301 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6361 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6421 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6481 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6541 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6601 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6661 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6721 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6781 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6841 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6901 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6961 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     7021 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     7081 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     7141 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     7201 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     7261 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     7321 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7381 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7441 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7501 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7561 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7621 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7681 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7741 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7801 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7861 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7921 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7981 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     8041 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     8101 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     8161 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     8221 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     8281 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     8341 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8401 aagatgtata aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8461 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8521 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8581 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8641 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8701 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8761 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8821 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8881 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8941 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     9001 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     9061 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     9121 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     9181 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     9241 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     9301 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9361 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9421 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9481 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9541 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9601 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9661 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9721 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9781 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9841 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9901 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9961 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
    10021 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
    10081 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
    10141 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
    10201 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
    10261 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
    10321 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10381 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10441 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10501 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10561 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10621 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10681 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10741 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10801 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10861 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10921 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10981 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    11041 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    11101 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    11161 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    11221 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    11281 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    11341 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11401 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11461 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11521 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11581 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11641 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11701 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11761 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11821 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11881 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11941 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    12001 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    12061 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    12121 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    12181 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    12241 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    12301 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12361 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12421 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12481 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12541 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12601 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12661 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12721 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12781 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12841 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12901 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12961 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    13021 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    13081 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    13141 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    13201 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    13261 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    13321 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13381 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13441 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13501 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13561 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13621 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13681 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13741 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13801 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13861 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13921 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13981 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    14041 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    14101 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    14161 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    14221 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    14281 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    14341 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14401 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14461 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14521 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14581 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14641 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14701 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14761 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14821 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14881 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14941 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    15001 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    15061 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    15121 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    15181 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    15241 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    15301 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15361 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15421 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15481 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15541 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15601 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15661 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15721 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15781 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15841 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15901 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15961 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    16021 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    16081 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    16141 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    16201 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    16261 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    16321 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16381 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16441 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16501 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16561 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16621 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16681 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16741 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16801 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16861 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16921 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16981 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    17041 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    17101 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    17161 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    17221 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    17281 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    17341 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17401 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17461 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17521 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17581 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17641 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17701 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17761 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17821 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17881 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17941 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    18001 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    18061 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    18121 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    18181 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    18241 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    18301 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18361 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18421 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18481 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18541 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18601 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18661 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18721 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18781 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18841 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18901 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18961 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    19021 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    19081 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    19141 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    19201 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    19261 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    19321 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19381 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19441 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19501 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19561 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19621 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19681 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19741 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19801 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19861 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19921 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19981 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    20041 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    20101 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    20161 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    20221 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    20281 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    20341 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20401 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20461 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20521 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20581 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20641 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20701 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20761 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20821 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20881 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20941 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    21001 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    21061 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    21121 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    21181 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    21241 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    21301 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21361 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21421 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21481 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21541 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21601 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21661 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21721 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21781 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21841 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21901 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21961 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    22021 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    22081 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    22141 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    22201 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    22261 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    22321 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22381 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22441 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22501 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22561 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22621 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22681 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22741 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22801 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22861 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22921 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22981 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    23041 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    23101 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    23161 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    23221 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    23281 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    23341 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23401 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23461 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23521 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23581 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23641 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23701 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23761 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23821 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23881 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23941 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    24001 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    24061 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    24121 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    24181 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    24241 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    24301 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24361 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24421 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24481 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24541 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24601 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24661 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24721 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24781 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24841 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24901 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24961 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    25021 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    25081 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    25141 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    25201 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    25261 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    25321 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25381 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25441 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25501 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25561 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25621 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25681 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25741 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25801 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25861 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25921 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25981 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    26041 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    26101 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    26161 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    26221 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    26281 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    26341 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26401 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26461 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26521 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26581 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26641 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26701 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26761 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26821 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26881 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26941 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    27001 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    27061 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    27121 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    27181 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    27241 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    27301 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27361 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27421 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27481 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27541 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27601 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27661 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27721 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27781 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27841 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27901 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27961 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    28021 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    28081 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    28141 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    28201 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    28261 agcgcgggtt ccagcgcagt gcctgcatta cccggagcag caaaaacagt ctcaaccagc
    28321 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28381 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28441 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28501 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28561 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28621 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28681 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28741 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28801 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28861 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28921 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28981 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    29041 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    29101 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    29161 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    29221 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    29281 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    29341 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29401 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29461 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29521 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29581 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29641 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29701 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29761 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29821 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29881 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29941 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    30001 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    30061 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    30121 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    30181 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    30241 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    30301 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30361 ttcggttgaa ga
//
LOCUS       DADLTY010000030        30295 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.030, whole genome shotgun sequence.
ACCESSION   DADLTY010000030 DADLTY010000000
VERSION     DADLTY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.030"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="JN334_001141"
     CDS             complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="JN334_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HAY7437361.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="JN334_001142"
     CDS             complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="JN334_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HAY7437362.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="JN334_001143"
     CDS             complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="JN334_001143"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HAY7437363.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="JN334_001144"
                     /pseudo
     CDS             complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="JN334_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="JN334_001145"
     CDS             complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="JN334_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HAY7437364.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="JN334_001146"
     CDS             complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="JN334_001146"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HAY7437365.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6041..7180)
                     /locus_tag="JN334_001147"
     CDS             complement(6041..7180)
                     /locus_tag="JN334_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY7437366.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7839..9422
                     /locus_tag="JN334_001148"
     CDS             7839..9422
                     /locus_tag="JN334_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY7437367.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="JN334_001149"
     CDS             complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="JN334_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HAY7437368.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="JN334_001150"
     CDS             complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="JN334_001150"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAY7437369.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12422..13063)
                     /gene="udk"
                     /locus_tag="JN334_001151"
     CDS             complement(12422..13063)
                     /gene="udk"
                     /locus_tag="JN334_001151"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAY7437370.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13381..13623
                     /locus_tag="JN334_001152"
                     /pseudo
     CDS             13381..13623
                     /locus_tag="JN334_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13622..14563
                     /gene="mdtA"
                     /locus_tag="JN334_001153"
                     /pseudo
     CDS             13622..14563
                     /gene="mdtA"
                     /locus_tag="JN334_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14563..17685
                     /gene="mdtB"
                     /locus_tag="JN334_001154"
                     /pseudo
     CDS             14563..17685
                     /gene="mdtB"
                     /locus_tag="JN334_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17686..20751
                     /gene="mdtC"
                     /locus_tag="JN334_001155"
     CDS             17686..20751
                     /gene="mdtC"
                     /locus_tag="JN334_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HAY7437371.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20752..22167
                     /locus_tag="JN334_001156"
     CDS             20752..22167
                     /locus_tag="JN334_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7437372.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22164..23567
                     /gene="baeS"
                     /locus_tag="JN334_001157"
     CDS             22164..23567
                     /gene="baeS"
                     /locus_tag="JN334_001157"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HAY7437373.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23564..24286
                     /gene="baeR"
                     /locus_tag="JN334_001158"
     CDS             23564..24286
                     /gene="baeR"
                     /locus_tag="JN334_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HAY7437374.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24477..24809
                     /locus_tag="JN334_001159"
     CDS             24477..24809
                     /locus_tag="JN334_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAY7437375.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24956..26317
                     /locus_tag="JN334_001160"
     CDS             24956..26317
                     /locus_tag="JN334_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HAY7437376.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26659..26976)
                     /locus_tag="JN334_001161"
     CDS             complement(26659..26976)
                     /locus_tag="JN334_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437377.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27382..28281
                     /gene="yegS"
                     /locus_tag="JN334_001162"
     CDS             27382..28281
                     /gene="yegS"
                     /locus_tag="JN334_001162"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HAY7437378.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28363..29142)
                     /locus_tag="JN334_001163"
     CDS             complement(28363..29142)
                     /locus_tag="JN334_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7437379.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29242..30282)
                     /gene="gatD"
                     /locus_tag="JN334_001164"
     CDS             complement(29242..30282)
                     /gene="gatD"
                     /locus_tag="JN334_001164"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY7437380.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         7009 a         8049 c         7829 g         7408 t
ORIGIN      
        1 cctaaatcgc ccagacgggt ggaaatataa ggaaacgcga ttaccacacc aacccggaat
       61 acagccagag aaatcaatgc taacggtaat tttttcttaa tcgcctcttt attcagatat
      121 tgaaatgccc actccagcaa ataaaacagg ataaaggtca taacccctga aaacgatccg
      181 gataatatta tccctgcgag aatcagggca tctgttttag gcgttttgat accaaactgt
      241 ttgatgctga gccaaattga gattaatgcc agagcgaaaa atgccggttc gaaataaagt
      301 gctgtcgtgc gcttgccgcc gaatttaatg aaattcagca catagctgtt gctgtaaatc
      361 agatatttcg aaatcgactc cataatactg ctgccaccgg tgaggataat ttgcgccatc
      421 tccaccgccg ccagcgccac caccagtccg accaccagat aaaagaaacg taatatctta
      481 cgatggttgt gtggcgaaat tgttttaaaa cgaatactcc acaccatgcc aataatgatc
      541 acaatataga caaacagcat agttgaagtg acgtatttac cggcatccaa cgactgacca
      601 aacaggtagt taaacgccgt gagtcccgcg ccaatcccta aggcaatcat caatttttta
      661 atgctgatgc gttctaaaaa cagcagtaac aagacaggta aaaaagtgac gatggtgatg
      721 gggaaacttt cgccaagctg ggcgattttg acgttaacca gcaggtagat cagtggcagc
      781 agcaggtagc tacagattct gatagaagtt gacatactcc tccagcatct gttgtccact
      841 gtaggcggcg cgactgcgtt ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc
      901 gatttccggt ttgcttaact gtaccagttg cagcacctct tcttcactga cggttttacc
      961 accggatttt tgcagcactt cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc
     1021 aatcgatagc gcctcacaca aaatcagcgg gtagttatcg acacgagaac tgaacaccag
     1081 cgcatccatc tgattgagcg cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt
     1141 aaccacgttg ccagcggtga acggcgagaa cttaccaaag gtatgcagtt cgattttgtc
     1201 gcccagcgcc atcatctcac gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc
     1261 atgcgccacc actgcgattt tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc
     1321 cagaatcgct tcggttgcca tatcaatgcc attattgata atccggcaac gccctggccc
     1381 gtacagacta ttgaaagcgt cagccacatg ctggctgggg gaaataaact gacagcccag
     1441 cgccagcatc tcgcggaata actggcgttt gcccgccacc agctggtgcg cgcgatcaat
     1501 cttcaccggc ggatagttat ttaaggtcgg gcatttctgg cagcccgttt tccagccttc
     1561 gcaaccgtcg gtaaaggcgc agcgtccggt tacactccag tggtcgtgca gcgtccagac
     1621 cagggtgaca tccggtttat ggtttttcac tttttcgcaa aagcgcacca cgctgttaag
     1681 attcaaccag tagctgtgca gcacatgaaa atgcaggacc accggacccg gtgtgcgagt
     1741 aatggtgcga tataactcat tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa
     1801 cgcaatgttc gccatcgcgg tcatccgcgg cgtatgtttg atgacctgcg gatagttctg
     1861 atggctgacg ctctctttgc cgcctttgcc gtagccgtag acaaaatgtg acgccagccc
     1921 ctgttgcagc gcacgctggt ggagatctaa cgccacacct gctgccccgc cttccgccag
     1981 tcgcacatta aattgcagaa tattcattta atcaccttca ctcgcgcttt ttctcccacc
     2041 accagcgcgt tgtccgggac agagtcgagc accacgctgc ccgcacccac ggtgacgttg
     2101 ttaccaagcg tgatatcacc aagaataatg acgttggcac cgagttcgac gccgttgcca
     2161 atgtgtggac atgccatgtt atcggcaccc cgattgccga tagtgacgcc gtggcgaatg
     2221 gtgaaatcat cccccgccac cacgttttta ttgatcacta cggcgtaacc gtgatggata
     2281 gtaaagcggc ggccaatggt cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg
     2341 ataatgcgat acagcaccag cagcggggcc gcccacagat tgttgaggac gttctttttg
     2401 cgccacaccg aacaaaaatg agcgacgcga taggcaagta ccatgcagca cgggcgtaaa
     2461 ctccagctgt tggcgcgcag atcttccagc atctttagcg cccccgaata ccatcagcca
     2521 gacgtttgcc gttacgcacc gacagcagcg tcaacaaggt gcgccaggtc atacgtttat
     2581 tgcggatctg gtagagggta aacagctgat attttttact ggcgcggtcg aatttgtctt
     2641 tgtgcttgcg gtaaaagtgg aagtatccgg agaatttttt cggtgacgag gtgatctgca
     2701 tttcgccgtg attgatatgc aggatctgcg tcgcctcttc cactttccac ggttcgccgt
     2761 actccaccac catccgcagg aatatgtcgt aatcctgtgc tgctttcagt tcggtatcga
     2821 acaaacactc tttgaaacgc catgcccagg taaagacctg attgccaatg atattacgtt
     2881 tatagaacag gcggcgtgag tacggcgatt tgggatacag tggtaagctc gccggttggg
     2941 aataaacttc tccctagcaa acgtagtcgt tagcgtacaa aaaggcgtgt gtgaccagtt
     3001 gctgtttatg ggcgaggaag acgctcaggc ggttgggcgt ccattcatca tcatcatcga
     3061 tcccggtgat gtattcccct tgcgccagca taatcgcctg gttacgcacc gcgcacgccc
     3121 cgctgttaat gtcgttgtgg atgtaagtga tacgcggatc gttgagggca gtgacgtact
     3181 gttgcagttg ttcccaggag gtggagcaat catccacgat gatcatctcc cagttgctgt
     3241 agtcctggcg caggaccgat tttatcgccc gaatcgccag ttgttggcgg ttccaggttg
     3301 gcatatagat tgagatcagc ggattgtctt tcataggttg ttctcccggc ttatgagctg
     3361 gtttgcttgc cggatgcggc gtgagcgcct tatccggcct acggggcggt gcgaatgcag
     3421 gcgtcgttat ttcgcatccg acttatattc gtattcgtaa tagccataat cctgatacgc
     3481 gctggcgcgg cggaagatgg agttcagaat cacccctttc accggaatac cgttttgctc
     3541 aaagcggctc agactggttt ccacttcttt caatgtgttg accgcataac gcgccaccat
     3601 taacgtggtt ccgacatgac gaccaacaat tgccgcatcg gtcactgcca gaatcggcgg
     3661 cgtatcaatc aacaccaggt catagttttt actcgcccag ttcaccagtt cggcaaagcg
     3721 ttcgctcatt aacagttcag aaggatttgg cggcacctga ccgcgcggga tcaggtcaaa
     3781 tttggcaata gaggtcggtt tagcagcggt agtaatatcg ccctgaccaa tcagaatctc
     3841 cgacagccca ttgacattat tggtgcccaa cagctcgtgg gtgtagcctt tgcgcatatc
     3901 gcagtcgatc aacaacacgc gtttattggt ctggctgatc accgccgcca ggttggcgca
     3961 gacaaaggtt ttaccgattg acgggctaac cccggtcatc atcaacacat tgttctgcgc
     4021 ctgcatcatc gcgaagtgca gactggtacg cagactacgg atggcttcaa tcgccagatc
     4081 ggttggattc cccactgcca gtagctggct ctgtttatag cgtttaaccc ctttgatggt
     4141 tttgacgcta tcgcgcgctt tctgccactc cgacagcggg atgctggcat agacgctgat
     4201 tccgtgttct tccagcactt gcgggctttc gatgccgcga ttaaacaacg agcgcagcag
     4261 cacacccacg atagagagca tcaggccaag gataatcgcg ccgaggataa tcagcccttt
     4321 cttcggtttt agcacgccag gctgggtgat tgccgggtca ataatgcgca catcgccgac
     4381 ggtgctggct tcggtgattt tcagctcctg ctctttattc agcagttgca tatagacctg
     4441 ctgaccagac tcgacatcgc gggtcagacg gacaatttcc tgctggtttt tcggcatcgc
     4501 cgttacgcga ccgttaagtt tggctttctc ttcttccagc gcctgacgtt tctccagcag
     4561 cgtgcggtac gccgggtgaa ctttggtgta cagcttggag atttccgcct ctttaaaggt
     4621 cagttcgttc aactgcgcgt cgatgttcac catcgaatcg agcaccgctt tcgcttcaag
     4681 cggcagatca acagaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc
     4741 gaggcggcta cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc
     4801 cgccgatttg cgctcaatat tttgttcctg atagttacga gcgatgctgt taagaatgtc
     4861 gcggatctgt tcgcgatctt caccggtata agtcaggctc agtacgcctg cgtctttgcc
     4921 gttctccgtt accgtcaggc tgttttgcag ttggttgatc atccccaacg tggagtattt
     4981 ggtgacggta aactcactgc ccgggcgggc gtgaatggct tcaaccatca gcgtgacgcc
     5041 ttcttttttc agcatctggc ccgcttgccc acgggcgcta aagccgccat cactgctcag
     5101 ggtgtagttt ttgtcatcca gcacattaag cgtaaacacc tgatccgcca tctctttcgg
     5161 gcggttaaag gtagtcactt tcaccgtctc gttctgacgc cccatcaggc gatcccagcc
     5221 cgccccgaaa atcgggaacg tgtttttgct caccgcaata tcgaggtcga gatcgtccac
     5281 cgttttacca agcaccagac gcgaacgaat caactggatc tcggcgtccg atgcaggcgg
     5341 tttgttggct aatgccgagc cgatatcctg caccagcgaa ttgccgctgt tttgctcgat
     5401 ttgtaccagt gcgtcggcac tataaatcgg cgtggcgaag aaagtgtaaa ccacggcaca
     5461 gagggcaaat acgacggtga tgccaatcac ccaccagcgc gcttcaatga cggtgccgac
     5521 caggcgacca atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt
     5581 ttctgtcatt cttatacctg ctctgcgttc aatgcctgcg cccactggcg ggcagaccgt
     5641 tcaagtaatg tgtacaccgc tgcaaacgtt tcccggcttt tgtgatacgg atcggggatt
     5701 tcacattcgt tatcccagtg accaaacagc atcactttgc cgcgcatctc cggtgccatc
     5761 tcgcataagc gttcgatatg gcgcttatcc atggtcaaaa tcaggtcata gttgcgacac
     5821 aggcggcggc tgatttgtcg tgcacagtgg ccttccagag acagttgatg ttctgcggcg
     5881 acgctgatag cggtaggatc agcgccctta ccgaccagcg cgccgagtcc agcggactcc
     5941 actttcagtt ccgggtgata acgttgcagt aagcgttccg ccatcgggga acggcaaata
     6001 ttgccgacac agacaactaa gatgttgtta aacatgacga ttaccagtta tgaatgtcgc
     6061 tggctgtatc cgtcatgtaa cggacaccgc taatagttgg cagcaactga ttgatcagac
     6121 ggttccagcg ggaaaccggg gcggtggtga catacaccac gtcataaggt tgcaggcgga
     6181 attctgtcgc catcaccagc gacgtcgcat cggacatatc cagctggtag atattggcaa
     6241 tcttgccgtt acgcccgccc tcgcctttca gcggacgaat gacaaagatg ccgctggcgt
     6301 tggaggtggt catgtcgata ccttcggcat tggccagagc ttcagtcagg gtcatgccgc
     6361 taaagtccat tttcagggtg ctctgtttct tcacttcacc catcacaaat actttcagat
     6421 catcattacg cggcacgtag agaatatcgc cgggataaag cagtcggttc tgattgaggt
     6481 cgccgttttg catcagcgcc tgtaaagaaa tgcgttcttc acgaccattg tgtgttagca
     6541 ccacgttgcg ccagtcagcg gtgtcggtca ggccacctgc ggcgttgatg gcgtcgagaa
     6601 tggtcagtgg tacgttggtg atcgcctgtt gaccggattt attcacctga cctgagatat
     6661 aggccttttg tgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg
     6721 tcgctaagcg cccggtaata tcactgcgga tttcagcgag cgttttcccg actacgtgaa
     6781 ccttgccgat atacgggtaa aacatagtgc cgtcaggctg tacccagttg ccggtgtcgc
     6841 tggagctgcg gtactgacca gctggcgtgg tgagttccgg gtgatcccag acggtgacat
     6901 taagaacgtc ccccggcccg acgcgatact ggtaattcgc gatctcgctt tccagcgtca
     6961 tattggggcg cgctacattc gggcgtgggc gtaattggtc aatcaggcgc ggggtcagcg
     7021 gataaacatt caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt
     7081 tgcccatcgt cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg
     7141 acaccaataa tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc
     7201 agagtaatta tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt
     7261 aattgaataa aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat
     7321 tgacagaata attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt
     7381 tagctaccaa tacactgatt gagcttaatt tttcaaaccc tctctgcata caatcgttga
     7441 tgagaaaggg ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa
     7501 tattggctcg ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta
     7561 acaattcggc aattggctat ttttaagaat tatattaagt atcattcaat atggttttta
     7621 ggagtttctt taggttgaca atatttaata ttgcgtctcc acatgcgata tttcttaaat
     7681 aatgttttat tattaccgct tttaattcag ggataatgtg aggttattac cctcaaataa
     7741 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
     7801 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
     7861 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
     7921 tggtctttat tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg
     7981 ttaccgggct gctgctggcg atgttaatgc gcctgttact gctagcgtca atctcctggc
     8041 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
     8101 taatcatgct gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac
     8161 ggctggaagg gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctggggcg
     8221 tggtgacgca aattgtggta ctggacgcca ttttctcact cgactcagtg attaccgccg
     8281 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
     8341 tgttgatggc cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc
     8401 tctgcttaag cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
     8461 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcactca
     8521 atcagttggc tatctttaac cggcgacgtt ttctttccgc taatcagacg ctacgccagc
     8581 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg
     8641 ccaaaaccgc gtccatgctg gtggatcatg gaaaccagca gatctttaat ccgcaggaac
     8701 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtaagcagc attatgacgt
     8761 cgcgccacga tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc
     8821 tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc
     8881 tcggtgttgt tcacgttatc gacctgctac aacagtcact gcgcggcgaa ccgctcaacc
     8941 tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttaccagccc
     9001 tggagcagtt ccgtaatgcc cgcacgcact ttgcttttgt ggtggatgag tttggctcgg
     9061 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg
     9121 aagtggaaga gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg
     9181 cgaatggtca tatgccgctg gaggatctgg tgcaatatgt gccgctgccg ctggatgaaa
     9241 aacgtgaata tcacaccatt gccgggctgt tgatggagta tttgcagcgt attccaaaac
     9301 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc
     9361 gcgtgcagaa ggtgcagatt ataccgctgc gtaaggatgg cgagatggag tacgaggtgt
     9421 gatggtatcg gccattttac ccaatgctgc gtaacgcctt ataacttcag caatgaagcg
     9481 ttgtctgtgg taaacggaca tcacagcggg ggtgtgggtc cggctgtaaa tcgccgaatc
     9541 gtttcctgta ggctggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat
     9601 gttacctcgc gcccgtaccc gatagccgaa aggaataaga tgagggaacc gcgccagcgg
     9661 cgatttaccg ccgggagccc gggttgccag ggtggtggcg attgagccac cctggcacgt
     9721 tcaccggctt gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat
     9781 cttacatatt cagaatataa tagtgccgcc gcgaacgcct gatgcgacgc tttacgcgtc
     9841 ttatcaggcc tacaggacag gtcacctggt aacctacatc ttctccagca acttcttcac
     9901 atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc
     9961 ttcgtcctgt agatgtttgc gcagtaactg atccacttgc aggctgtaat tgagttgctg
    10021 ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct
    10081 ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc
    10141 cagattcaac atgccttccc ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt
    10201 taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac
    10261 gttatcgaag ttttcagcgg ccttcacatc accgccatta cgctctaccg cttgctgaat
    10321 catctgctgg aagttcatcc cttccatacg tgtgtcggtc atctcaacat gcgcctgtcc
    10381 ttgccagttg tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag
    10441 tgacattttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc
    10501 aacgttctcc agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt
    10561 gcccggcagt gaaacctttc caccgttaag cttgccctgc agttgagtaa tttccagcaa
    10621 acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac
    10681 gttactggcc tgcaacaaaa tatcagctgt aaagccttgc agtccctgat aggccggttc
    10741 atcaatacgc gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc
    10801 ggcaccgttt tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg
    10861 cgggaattgc agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgtcc
    10921 gctcagcgta ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga
    10981 ctcctgccac tgggactgga aactcccctg cccctgaata ccttgttttg gtaaatcggc
    11041 accctgcaac tgccagttaa tttgttcaat agccgctgtt aaatcatgcg gataatcaga
    11101 agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc
    11161 ggagaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg
    11221 gattgtcacc tgctcgtcat cttcatgctg gaacaccaga acgctgtccg ccaccttaag
    11281 actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg
    11341 cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac
    11401 tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc
    11461 cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg cttgcgccct gggcggtgag
    11521 agacattcgc ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc
    11581 gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc
    11641 attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga ccaagagtat
    11701 catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc
    11761 gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta
    11821 aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg
    11881 acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca
    11941 gcccggatcg atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga
    12001 acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca gtgtcaccgc
    12061 cagcgccagc tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc
    12121 gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa
    12181 cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc
    12241 gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca
    12301 ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcac atcactccct
    12361 taacgccaat tacgtcaagg gcataccaac ttatcactga acggtacaca atttatcaag
    12421 cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc
    12481 cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca
    12541 tcgggcgcac ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt
    12601 taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg
    12661 agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga
    12721 tgaccttctt cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt
    12781 aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcaacaggc
    12841 tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat
    12901 ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt
    12961 gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc
    13021 cagcgatacc gataatgacg cactgatgag actgatcagt catatattta gcgacctgat
    13081 taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca
    13141 tccgtctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa
    13201 ttcccggtgg caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt
    13261 gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt
    13321 ttgtactagc ataaacacag aaacgaatac tggcgacctg gccttgcgga taaagcggta
    13381 ataagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc
    13441 agtttaggtt tagtctcgtt tatctttacc cttgtctcgc ttgagctttc gcagtttggc
    13501 acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat
    13561 gccgggcgca tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc
    13621 attccaggaa ggccagcagg tcaaagcagg cgatttactg gcagaaattg accccagcca
    13681 gttcaaagtt gcattagcac aaacccaggg ccaactggca aaagataaag ccacgcttgc
    13741 taacgcccgc cgtgatctgg cgcgttatca gcaactggca aaaaccaatc tcgtctcccg
    13801 tcaggagctg gatgcccaac aggcgctggt cagtgaaacc gaaggcacca ttaaggctga
    13861 tgaagcaagc gtcgccagcg cgcagttgca actcgactgg agccgcatca ccgcaccagt
    13921 cgatggtcgc gttggtctca agcaagttga tgttggtaac caaatctcca gtggtgatac
    13981 caccggaatt gtggtgatca cccagacgca tcctatcgat ttagtcttta ccctgccgga
    14041 aagcgatatc gctactgtag tgcaggcgca aaaagccgga aaaccgctga tggtagaagc
    14101 ctgggatcgc accaattcga agaaattaag tgaaggcacg ctgttaagcc tcgataacca
    14161 aatcgatgcc acaaccggta cgattaaagt gaaagcacgc tttaataatc aggatgatgc
    14221 gctgtttccc aatcagtttg ttaacgcgcg catgttagtc gacaccgaac aaaacgccgt
    14281 agtgatccca acagccgccc tgcaaatggg caatgaaggc cattttgtct gggtgctgaa
    14341 tagcgaaaac aaggtcagca aacatctggt gacgccgggc attcaggaca gtcagaaagt
    14401 ggtgatccgt gcaggtattt ctgcgggcga tcgcgtggtg acagacggca ttgatcgcct
    14461 gaccgaaggg gcgaaagtgg aagtggtgga agcccagagc gccaccactc cggaagagaa
    14521 agccaccagc cgcgaatacg cgaaaaaagg agcacgctcc tgatgcaggt gttacccccg
    14581 agcagcacag gcggcccgtc gcgcctgttt attatgcgtc ctgtcgccac cacgctgctg
    14641 atggtggcga tcttactcgc cgggattatc ggttatcgcg ccctgcccgt ttcggcgctg
    14701 ccggaagtgg actatccgac cattcaggtg gtcacgctct acccaggtgc cagcccggat
    14761 gtcatgacct ctgccgttac cgcgccgctc gaacgccagt tcgggcagat gtctggcctg
    14821 aaacagatgt cgtcgcaaag ttccggcggt gcgtcagtta tcactttgca gttccagcta
    14881 acattaccgc tcaatgtcgc cgagcaggaa gtgcaggccg cgattaacgc tgcgaccaac
    14941 ttgttgccga gcgatctgcc taacccgccg gtttacagca aagtgaaccc ggcagatccg
    15001 ccgatcatga cgctcgccgt cacctcaacc gccatgccga tgacgcaagt ggaagatatg
    15061 gtggaaaccc gcgtcgcgca gaaaatctcg cagatttccg gcgtcggcct ggtgacgctt
    15121 tccggcggtc agcgtccggc tgttcgcgtc aaacttaacg ctcaggcgat tgccgccctc
    15181 ggcctgacca gcgaaaccgt gcgcaccgcc attaccggcg ctaacgttaa ctcggcaaaa
    15241 ggtagcctcg acggcccttc ccgtgcggtc acgctttccg cgaacgacca gatgcaatcc
    15301 gccgaagagt atcgccagct aatcatcgcc taccagaacg gcgcgccaat tcgtctgggc
    15361 gatgtcgcaa ctgtagagca aggtgcagaa aacagctggc tcggcgcgtg ggcgaacaaa
    15421 gaacaggcca ttgtgatgaa tgttcagcgc cagcccggtg ctaacattat ctccaccgcc
    15481 gacagcattc ggcagatgct gccacagctc actgagagtc tgccgaaatc ggtgaaggtg
    15541 acagtgcttt ccgatcgcac caccaatatc cgcgcatccg tcgatgatac tcagtttgaa
    15601 ttgatgatgg ctatcgcgct ggtagtcatg attatctacc tgtttttgcg caatattccg
    15661 gcgaccatca ttcccggtgt tgctgtaccg ctgtcgttaa tcggcacttt cgcggttatg
    15721 gtgtttctcg atttttcaat caataacctg acactgatgg ctttaactat cgccaccgga
    15781 ttcgtggtcg atgacgccat cgtggtgatc gaaaacattt cccgctatat cgaaaaaggc
    15841 gaaaaaccgt tggcggcggc gctcaagggc gcaggtgaaa tcggctttac cattatctcg
    15901 ctgaccttct cactgattgc ggtgttgatc ccactgctgt ttatgggcga tatcgtcggg
    15961 cgactgttcc gcgaatttgc tattaccctg gcggtagcga ttttgatctc agcggtggtg
    16021 tcgctgaccc tgacaccgat gatgtgcgcg cggatgctca gccaggagtc gttgcgtaaa
    16081 cagaacagct tctcccgtgc ctcggaaaaa atgttcgaca ggataatcgc cgcctatggt
    16141 cgtggactgg cgaaagtgct gaatcatccg tggctgacct taagcgtggc actcagcacg
    16201 ctgctgctta gcgtgctgct gtgggtgttc attccgaaag gtttcttccc ggtacaggac
    16261 aacggcatta ttcagggcac tttgcaggca ccgcaatcca gctcctttgc caatatggcc
    16321 cagcgacaac gccaggtcgc ggacgtgatt ttgcaggatc cggcagtgca aagcctgacc
    16381 tcatttgttg gcgttgatgg cactaacccg tcgctgaata gtgcacgttt acaaatcaac
    16441 ctcaaaccgt tggatgaacg tgatgatcgg gtgcaaaaag tcatcgcccg tctgcaaacg
    16501 gcggtagata aagtgccggg cgtcgatctc ttcctgcaac caacgcagga cctgaccatc
    16561 gatactcagg tcagccgcac ccagtaccag tttaccttgc aggccacgtc actggatgcg
    16621 ctcagtacct gggtgccaca gttgatggaa aaactccagc aactgccaca gctttctgat
    16681 gtctccagcg actggcagga caaagggctg gtggcgtatg tcaatgttga tcgcgacagc
    16741 gccagccgtc tggggatcag catggcggac gtcgataacg ccctgtacaa cgcgtttggt
    16801 cagcgactga tttccactat ttatactcag gctaaccagt atcgcgtggt gctggagcac
    16861 aataccgaaa ataccccagg cctcgcggcg ctggatacca ttcgcctgac cagcagcgac
    16921 ggtggcgtgg tgccgttaag ctcaattgcc aaaattgagc agcgttttgc gccgctttcc
    16981 atcaaccatc tggatcagtt cccggtaaca accatctcct ttaacgtgcc ggataactat
    17041 tcgctgggcg atgcggtgca ggcgattatg gacaccgaaa agacgctgaa tctgccggtg
    17101 gatatcacca cgcaattcta gggcagcacc ctcgccttcc agtcggcgct gggcagcact
    17161 gtctggctga ttgtcgcggc ggtggtggcg atgtatatcg tgctcggcat tctgtacgag
    17221 agctttattc acccgatcac cattctctcg acgctaccca ccgcaggggt cggcgcgctg
    17281 ctggcattaa tgattgccgg tagcgaactg gatgtgattg cgattatcgg cattattttg
    17341 ctgatcggta ttgtgaagaa gaacgccatc atgatgatcg acttcgcgct ggctgctgag
    17401 cgcgagcaag gcatgtcgcc gcgcgatgca atctaccagg cttgtctgtt gcgttttcgt
    17461 ccgatcctga tgaccaccct ggcggctctg cttggcgcgc tgccgctgat gttgagtacc
    17521 ggggtcggcg cggaactgcg tcgtccgtta ggtatcggca tggtcggcgg tctgattgtc
    17581 agccaggtgc tgacgctgtt caccacgccg gtgatttatc tgctgttcga ccgcctggca
    17641 ttgtggacca aaagtcgctt tgctcgccat gaagaggagg cgtaagtgaa gttttttgcc
    17701 ctcttcattt accgcccggt ggcgacgatt ttactgtcgg tcgccattac cctgtgcggc
    17761 atactgggtt tccgtatgct gccggtcgcc ccgctgccgc aggtcgattt tccggtgatt
    17821 atggtcagcg cctcgctgcc cggtgcgtcg ccggagacaa tggcgtcttc cgttgccacg
    17881 ccgctggagc gctcacttgg gcgcattgcc ggggttagtg aaatgacctc cagcagttcg
    17941 ctcggcagca cgcgcattat tttgcagttt gattttgacc gggatatcaa cggcgcagcg
    18001 cgtgatgtgc aggcggcgat caacgctgca caaagtttgc tgcccagcgg gatgcccagc
    18061 cgcccgacct atcgcaaagc gaacccgtcg gatgcgccaa ttatgatcct cacgctgaca
    18121 tccgatactt attcgcaggg tgaactgtac gatttcgcct cgacgcagct ggctccgacg
    18181 atttcgcaaa tcgacggtgt cggtgatgtc gatgtcggtg gcagctcgct gcccgccgtg
    18241 cgcgtcgggc tgaccccgca ggcgctgttt aatcagggcg tgtcgctgga cgacgtacgc
    18301 actgccatca gcaatgccaa cgtgcgtaaa ccgcaggggg cgctggaaga tggcactcac
    18361 cgctggcaga tccagaccaa cgatgaactg aaaaccgccg ctgaatatca gccgctgatt
    18421 attcactaca acaacggcgg cgcggttcgt ctgggcgatg tggcgacggt gactgactca
    18481 gtgcaggatg tgcgcaacgc cgggatgacc aacgccaaac cggctatttt actgatgatc
    18541 cgcaaactgc cggaagccaa tattatccag acggtagaca gcatccgggc aaaattacca
    18601 gagttgcagg agaccattcc ggcggcgatt gatctgcaaa ttgcccagga tcgctccccc
    18661 accattcgcg cctcgctgga agaagtcgag caaacgctga ttatctcggt ggcgctggtg
    18721 attctggtgg tctttttatt cctgcgctcg ggtcgcgcca ctattattcc cgccgttgcg
    18781 gtgccggttt cgctgattgg tacgtttgcg gcgatgtacc tgtgcggatt cagtctcaat
    18841 aatctttcgc tgatggcgct caccattgct actggtttcg tggtggatga cgccattgtg
    18901 gtgctggaaa acattgcccg tcatctggaa gcggggatga aaccactaca ggccgcgctg
    18961 caaggtactc gcgaagtcgg ctttacggtg ctgtcgatga gtctgtcact ggtggcggtg
    19021 ttcctgccgc tgctgttgat gggcggattg ccgggccgac tgttacgcga gtttgccgtg
    19081 acgctttctg tcgccattgg tatttcgttg ctggtttccc tgacgctaac gccaatgatg
    19141 tgcggctgga tgctaaaagc cagcaagccg cgtgagcaaa agcgactgcg tggttttggc
    19201 cgcatgttgg tagccctgca acaaggctac ggcaagtcgc tgaaatgggt gctcaatcat
    19261 acccgtctgg tgggcgtggt gctgcttggc accattgcgc tgaatatctc gatcccgaaa
    19321 accttcttcc cggagcagga caccggcgta ctgatgggtg ggatacaggc ggatcagagt
    19381 atttcgtttc aggcgatgcg cggcaaattg caggatttca tgaaaattat ccgtgacgat
    19441 ccggcagtgg ataatgtcac cggctttacc ggcggttcac gagttaacag tgggatgatg
    19501 tttatcaccc tcaagccacg cgacgaacgc agcgaaacgg cgcagcaaat tatcgaccgt
    19561 ctgcgcgtga agctggcgaa agaaccgggg gcgaatctgt tcctgatggc ggtacaagat
    19621 attcgcgttg gtgggcgtca gtcgaacgcc agctaccagt acacgttgtt atccgacgac
    19681 ctggcggcac tgcgcgaatg ggagccgaaa atccgcaaaa aactggcgac gttgccggaa
    19741 ctggcggacg tgaactccga tcagcaggat aacggcgcgg agatgaatct ggtttacgac
    19801 cgcgacacca tggcacggct gggaatcgac gtacaggccg ccaacagtct gttaaataac
    19861 gccttcggtc agcggcaaat ctcgaccatt taccagccga tgaaccagta taaagtggtg
    19921 atggaagtgg atccgcgcta tacccaggac atcagtgcgc tggaaaaaat gttcgttatc
    19981 aataacgaag gcaaagcgat cccgctgtcg tatttcgcta aatggcaacc ggcgaatgcc
    20041 ccactatcgg tgaatcatca gggattatcg gcggcctcga ccatctcgtt caacctgccg
    20101 accggaaaat cgctctcgga cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggt
    20161 gtgccttcga cggtgcgcgg cagttttgcc ggcacggcgc aggttttcca ggagacgatg
    20221 aactcgcagg tgatcctgat tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc
    20281 ctttacgaga gttacgtaca tccgctgacg attctctcca ccctgccctc ggcgggcgtt
    20341 ggagcgctgt tggcgctgga gctgttcaat gccccgttca gcctaatcgc cctgataggg
    20401 atcatgctat taatcggcat cgtgaagaaa aacgccatta tgatggtcga ttttgcgctt
    20461 gaagcccaac ggcacggtaa cctgacgccg caggaagcta ttttccaggc ctgtctgctg
    20521 cgttttcgcc cgattatgat gaccaccctg gcggcgctgt ttggcgcgct gccgctggta
    20581 ttgtcgggcg gtgacggctc ggagctgcgg caacccctgg agatcaccat tgtcggcggt
    20641 ctggtgatga gccagctcct gacgctgtat accacgccgg tggtgtatct ctttttcgac
    20701 cgtctgcggc tgcgtttttc gcgtaaacct aaacaaacgg taaccgagta aatgacagat
    20761 cttcccgaca gcacccgctg gcaattatgg attgtggctt tcggcttctt tatgcagtcg
    20821 ctggacacca ccatcgtaaa caccgccctt ccctcaatgg cgcaaagcct cggggaaagt
    20881 ccgttgcata tgcacatggt cattgtctct tatgtgctga ccgtggcggt gatgctgccc
    20941 gccagcggct ggctggcgga caaagtcggc gtgcgcaata ttttctttac cgccatcgtg
    21001 ctgtttactc tcggttcact gttttgcgcg ctttccggca cgctgaacga actgttgctg
    21061 gcacgcgcgt tacagggcgt tggcggtgcg atgatggtgc cggtcggcag gttgacggtg
    21121 atgaaaatcg taccgcgcga gcaatatatg gcggcgatga cctttgtcac gttacccggt
    21181 caggtcggtc cgctgctcgg tccggcgctc ggcggtctgc tggtagaata cgcgtcgtgg
    21241 cactggatct ttttgatcaa cattccggtg gggattatcg gtgcgatcgc cacattgatg
    21301 ttaatgccga actacaccat gcagacgcgg cgctttgatc tttccggctt tttattgctg
    21361 gcggttggca tggcggtatt aaccctggcg ctggacggca gtaaaggtac aggtttatcg
    21421 ccgctggcga ttgctggcct ggtcgcagtt ggcgtggtgg cactggtgct ttatctgctg
    21481 cacgccagaa ataacaaccg cgccctgttc agtctgaaac tgttccgcac tcgtaccttt
    21541 tcgctgggcc tggcggggag ctttgccgga cgtattggca gtggcatgtt gccctttatg
    21601 acaccggttt tcctgcaaat tggcctcggt ttctcgccgt ttcatgccgg actgatgatg
    21661 atcccgatgg tgcttggcag catgggaatg aagcgaattg tggtacaggt ggtgaatcgc
    21721 tttggttatc gtcgggtact ggtagcgacc acgctgggtc tgtcgctggt caccctgttg
    21781 tttatgacta ccgctctgtt gggctggtac tacgttttgc cgttcgtcct gtttttacaa
    21841 gggatggtca actcgacccg tttctcctcc atgaacaccc tgacgctgaa agatctcccg
    21901 gacaatctgg cgagcagcgg aaacagcctg ctgtcgatga ttatgcaatt gtcgatgagt
    21961 atcggcgtca ccatcgccgg gctgttgctg gggctttttg gttcgcagca tatcagcgtc
    22021 gacagcggca ccacacaaac cgtctttatg tacacctggc ttagcatggc gtttatcatc
    22081 gcccttccgg cgttcatctt tgccagagtg ccgaacgata cgcatcaaaa tgtagctatt
    22141 tcgcggcgaa aaaggagcgc gcaatgaagt tctggcgacc cggtattacc ggcaaactgt
    22201 ttctggcgat tttcgccacc tgcattgtct tgctgatcag tatgcactgg gcggtgcgta
    22261 tcagttttga gcgtggcttt attgattaca tcaaacacgg caatgaacag cgattacagc
    22321 tgttaagtga tgcgcttggc gagcagtatg cgcagcatgg taactggcgc ttcctgcgca
    22381 acaacgatcg ctttgtcttt cagatcctgc gttcatttga acacgataat tcggaagata
    22441 aacccggccc gggtatgccg ccgcacggct ggcgtaccca gttctgggtg gtcgatcaaa
    22501 acaacaaagt actggttggt ccgcgagcgc cgattccacc tgacggtaca cggcgaccca
    22561 ttctggtcaa cggtgcggaa gtgggcgcgg tgattgcctc ccctgttgag cggctgaccc
    22621 gcaatactga tatcaatttc gataaacaac agcggcaaac cagctggctg atcgtcgccc
    22681 tggcaacgtt actcgcggcg ctcgccacct ttctgctggc gcgcggtttg ctggcaccgg
    22741 taaaacgact tgtcgatggc acgcacaaac tggcggcggg cgatttcact acccgcgtaa
    22801 cgcccaccag tgaagatgaa ctgggcaaac tggcgcaaga cttcaaccag ctcgccagca
    22861 cgctggagaa aaaccagcaa atgcggcgcg attttatggc cgatatttct cacgaactgc
    22921 gtacgccatt agcggtgctg cgcggtgaac tggaagccat tcaggatggc gtgcgcaaat
    22981 tcacgccgga gacggtggcg tctttacagg cggaggtcgg tacactgacc aaactggttg
    23041 acgatcttca tcagttgtcg atgtctgatg aaggcgctct cgcctatcaa aaagcaccgg
    23101 tagatttgat cccactgctg gaagtggcgg gcggcgcatt tcgcgaacga ttcgccagcc
    23161 gtggcctgaa actgcaattt tccctgccag acagtatcac cgtatttggc gatcgcgacc
    23221 gtttaatgca gttattcaat aacttactgg aaaacagcct gcgctacact gacagcggcg
    23281 gcagcctgaa aatctctgcc gagcagcacg acaaaacggt gcgcctgacc tttgccgaca
    23341 gtgcgccagg tgtcagtgac gatcagctac aaaaattgtt tgaacgtttt tatcgcaccg
    23401 aaggttcccg caaccgcgcc agcggcggtt ccgggctggg gctggcgatt tgcctgaaca
    23461 ttgttgaagc ccataatggc cgcattattg ccgcccattc gccttttggc ggggtaagca
    23521 ttacagtaga gttaccgctg gaacgggatt tacagagaga agtatgaccg agttaccaat
    23581 cgacgaaaac acaccgcgta ttttgatcgt ggaagatgaa ccgaagctgg ggcagttgct
    23641 cattgattat ctgcgtgctg cgagctatgc gccgacgctt atcagccacg gcgatcaggt
    23701 actggcgtat gtgcgccaga caccaccgga tctgatcctg ttggatctga tgctccctgg
    23761 caccgatggc ctgacattat gccgggaaat tcgtcgtttt tccgatgttc cgatcgtgat
    23821 ggtgacggca aaaatcgaag agatcgaccg cctgctgggg ctggagattg gcgccgatga
    23881 ctacatctgc aagccgtaca gcccacggga agtggtagcg cgtgtcaaaa ccattttgcg
    23941 ccgttgtaaa ccgcaacgcg aacttcagca acaggatgcc gaaagcccgt tgattatcga
    24001 cgaaggtcgt tttcaggctt catggcgcgg taaaatgctt gacctgacgc ctgcggaatt
    24061 tcgtctgctt aaaacgctct ctcacgaacc aggaaaagtg ttctcccgcg agcaattgct
    24121 caatcatctt tatgacgact accgcgtagt aaccgaccgc accattgaca gccacattaa
    24181 aaatctgcgc cgcaagctgg aatctctcga cgccgaacag tcatttatcc gcgccgttta
    24241 cggcgttggt taccgctggg aagccgacgc ctgccgcatc gtttagtttt agcgacatta
    24301 ctttgttagc cggagatggc gttcagaccc gatccggcaa cattattcca cgcatggtca
    24361 gcaaactgac attatctctc cgccctggca ggcatcagaa aatcgctcat actttaatca
    24421 gtaaacagca cctttagatg ctgttttcga taagcaattt caatcaagga gtcattatgg
    24481 ctggttggtt tgaactcagt aagagcagtg ataatcagtt ccggtttgtg ctaaaagcgg
    24541 gcaatggtga gactatcctc accagcgagc tgtatacctc aaaagcctct gcggaaaagg
    24601 gcatcgcgtc ggtgcgtagc aacagcccgc aagaagagcg ctatgagaaa aaaacggcaa
    24661 gcaacggcaa attctatttc aatctgaaag ccgctaatca tcaaattatc ggctccagcc
    24721 agatgtacgc caccgcgcaa tctcgtgaaa ccggaattgc ctccgttaaa gccaatggca
    24781 caagccagac ggtgaaagac aatacgtaat tacgatgccg ggcgtgggtt gttggcgtcc
    24841 ggcgctttac cagatccccc ctacagcgct acaatgcccg cccttaaagt gggggcctcc
    24901 cctaaccgct tcatcaggtg aagcggatct gacctgtcat cagaacgaga gaattatgtt
    24961 taaaccggaa ctcctttccc cggcgggaac gctgaaaaat atgcgttacg ctttcgctta
    25021 tggcgcagat gctgtttatg cgggccagcc gcgttactcc ctacgtgtgc gcaacaacga
    25081 attcaaccac gaaaatcttc agctcggcat caatgaagcc cacgcgctgg ggaaaaagtt
    25141 ttatgtcgtg gtcaacattg caccgcacaa cgccaagctg aaaaccttta tccgtgacct
    25201 gaaaccggtg gtggaaatgg ggccggatgc gctgattatg tccgatccag ggctgattat
    25261 gctggtgcgt gagcacttcc ctgaaatgcc gatccacctc tcggtgcagg ctaacgccgt
    25321 gaactgggcg acggtgaaat tctggcagca aatgggactg acccgcgtga tcctctctcg
    25381 cgagctgtcg ctggaagaga ttgaagagat ccataatcag gtgccggata tggagatcga
    25441 gatcttcgtt cacggcgcac tgtgcatggc ctactccggt cgctgcctgc tctctggcta
    25501 tatcaacaag cgcgacccga accagggcac ctgcaccaac gcctgccgct gggagtacaa
    25561 cgtccaggaa gggaaagaag atgacgttgg caacatcgta cacaagtacg agccgattcc
    25621 ggtgcaaaat gttgagccga cgctgggtat cggcgcgcca accgacaaag tgtttatgat
    25681 cgaagaagcc cagcgtccgg gcgagtatat gaccgcgttt gaagatgagc acggcactta
    25741 catcatgaac tcgaaagatc tgcgcgccat cgcccatgta gaacgcctga ccaaaatggg
    25801 cgtgcattcg ctgaaaatcg aaggtcgtac caaatctttc tactattgcg cacgcaccgc
    25861 acaggtttac cgcaaagcta tcgatgacgc cgctgcggga aaaccgttcg ataccagcct
    25921 gctggaaact ctggaaggtc tggcgcatcg tggctatacc gaaggtttcc tgcgtcgtca
    25981 tactcacgac gattatcaga actacgaata cggttattca gtttctgacc gccagcagtt
    26041 tgttggtgag tttaccggtg agcgcaaggg ggacctcgca gcggtagcgg tgaaaaataa
    26101 attctccgtt ggcgacagtc ttgagctgat gacgccgcaa ggcaacatta actttaccct
    26161 tgagcacatg gaaaacgcca aaggcgaagc tatgccggtc gcaccaggcg atggttatac
    26221 tgtgtggctc ccggtcccgc aggatcttga gctaaattac gcgctgctaa tgcgtaattt
    26281 ctccggggaa accacgcgta acccccacgg taagtgatta atttcgatta tttttcccgg
    26341 atggaaaatt cttagaaacc gatcacatac agctgcattt attaaggtta tcatccgttt
    26401 cgctgaaaaa cataatccat aaaatgctag ctgtaccagg aaccacctcc ttagcctgtg
    26461 taatctccct tacacgggct tattttttgt acataagctg taaagaagga ttgatccctg
    26521 ataaccgtct tccattgacc acatcgatag aatccccctt catagcacaa tgcctttcac
    26581 gtaacggtat cgtgctcgcc caggttccgg ctacgcacag ccagaacgcg catgtttgac
    26641 gcttacaaaa aaatattctc actctccaca tttgaatgtc agacgagcga cgccatgtaa
    26701 tcctgcacct tctgtcttca ggtcaactat ctgcattttt ttgccctgag taacacagaa
    26761 atgggctgca tcatttttta ctatattttc tgcaccagat attctgcccc tggctaaaga
    26821 agcttcggct tcggtgtagt attggttatc gagtttacgc tgaatattac ttttatatgc
    26881 aagaccaaat ttaccgatac ttgtttcatc attatgcaca gcacaaccag atataagaaa
    26941 aatactaatt aatgatatag cagctatctt tttcatctca ccttccccca ttaaatacca
    27001 acgacactct ctagtgttta aatataataa tggcatgatt attataattg aataggatta
    27061 taataaatgt tctgtacaac atttcctaca taagtaggaa ttacggacat tgaggccctt
    27121 cagggtaact ccatgggggc tttaatatat tatattgaag atgccactgt ttagttgaat
    27181 attaggtata tgctcttttt tgaaatttat cggtggcagc cgttagtatt cgctgtcccc
    27241 attgcaagct cctggtggta accactgaat cctccatact tgaactgact ttttatcctc
    27301 cgactttcat cctgttctga ctccaccttt tgttttctgc tctacactat ctacagacca
    27361 atcataaagg cacatacgat catggcagaa tttcccgcca gcttactgat tcttaatggt
    27421 aaaagtactg acaatctacc cttgcgcgaa gcaattatgc tgttgcgtga ggaaggaatg
    27481 acgatccatg tgcgggtcac ctgggagaaa ggtgatgccg cacgatttgt agaggaggcc
    27541 cggaagttgg gcgtcgcaac ggtgattgcc ggtggtggcg atggcaccat taatgaagtt
    27601 tctacggcgt tgattcagtg tgagggggat gacatacccg cgctgggaat tttgccatta
    27661 ggaaccgcca atgattttgc caccagtgta gggattcctg aggcactgga taaggcgctg
    27721 aaactggcaa ttgccggtaa tgccattgcg atagatatgg cgcaggtcaa caaacaaacc
    27781 tgttttatta atatggcgac aggcggattt gggacgcgta ttaccacaga aacgccggaa
    27841 aaattaaaag ccgcgctggg tggcgtctct tacatcattc atggcttaat gcgcatggat
    27901 actctgcaac cggaccgttg tgaaatccgc ggtgaaaact ttcactggca aggtgacgcc
    27961 ctggtcattg gtattggtaa cgggcgtcag gccggtggcg gtcaacaatt gtgcccgaac
    28021 gcgttaatta acgatggctt gctgcaactg cgcattttta ccggcgatga aattcttccg
    28081 gctctcgtat caaccttaaa atctgacgaa gataacccga atattatcga aggcgcttcg
    28141 tcgtggtttg atatacaagc cccacacgaa atcactttta atcttgatgg cgaaccgttg
    28201 agtgggcaaa acttccatat tgaaatactt ccggcggcgt tgcgttgtcg attaccacca
    28261 gattgtccat tattgcgtta atcaaactat tctctcttga accagggaga atccgtaatg
    28321 ccggatgcgg tgtaaacacc ttatccggca tacagaacaa tactacaccg taattaattt
    28381 tacccgtgtc ttctgcaatg ccaatttatc cacctctgac acagcatcat cggtaacaat
    28441 aatgtcaatt ttttcgattg gcagtacctg attaaaaccg cgacgattaa atttcgatga
    28501 atcgagaacg gcaatcactt tatttgcggc ggtgaccatc gccccgctaa tcgaataacc
    28561 ttcattaaag gtcgtaatac cattaaccgc atcaatacca tcagcaccga caaacattaa
    28621 atcggcatta atatcctgca atgaacgctc ggcaatggaa ccatgcatcg agcgcgtttt
    28681 atggcggacc gtgccaccac agacgaccag agtaatgtct ttattttcgg aaagggcaaa
    28741 cgctgccggg agactgttgg taatcaccgt gatattttta gcggtcatta atccttcggc
    28801 gataagcatt gtggtacttc cgctgtcgag aataacggtc atcccttcat ggatcatttt
    28861 gactgccgcc tgcgctattc tgttttttgg cgcgctggcg agctgaaatc gctctttaaa
    28921 cccgacttcc tgggtctcgg tttcactatt accggacatt attttcgccg caccgccatg
    28981 aaagcgcgta accacgcctt tttgttcgag aaagcgcaaa tcggcacgga ttgtcgcttc
    29041 cgaggcagca aatactcccg ccagatcctg aacaagcacg gttccctgtt cattcactaa
    29101 ttggatgatc ttattccttc gctcgaatga attcatcgtc atttacccat attcaattgt
    29161 ggctagtgta aacgaagggg gaccattggt gaatgatctg ttttgcacaa taccgggcca
    29221 gcatcacgct ggcccgcggt ttcatggaat gagcaacact ttgccctgca tggcattacg
    29281 agcgatgtca cgcaccgcct gggtgaagct ttcaaagctt ccacggtgag cgattaatgg
    29341 ctccaggctt aacttacgtt ctgtcagcaa ccggctcgcc gtttcccact cctgccccgg
    29401 ccaagggctg gaatagttca tccaactgcc gataaccgtc agctctttac gcaatatttt
    29461 gccaaacgtt gccgatgtta aatgcagatc ctgatgcaac gtgcccacca gcgccagttg
    29521 ggcatgagga ccggcaatct ctaccgccag ttcgacagtt tgcggtacgc cagccgtctc
    29581 gaggataagc tgattaaagc gcagttcgcg taaaacgctc tgcatttgcg gcgcgctcat
    29641 ttcgctactg ttaaatgttt gcatcgcacc gaaagatttt gccagtgcca gtttttctga
    29701 gctaatgtcg atcgccgtca cactctttgc tcccagcgcg acagcgcact gaatagccag
    29761 caggccaatg gttccggcac caataataat aacgttttta ttctcacaac cttgcgctaa
    29821 atgaaaagca tgcaggccaa cggtaatcgg ctcaataaaa gccccatcct caataggcat
    29881 atccgtgggt agagcaaaga cattttttcg cttaacgaca atatattcag caaatccacc
    29941 atcacgccgc gagccaataa aatcatattt tgcgcactgg gaataaaacc ctttcaaaca
    30001 ctctggacaa gtaaaacagg gtaataacgg cacacaggca accgcatcgc cagggtgtaa
    30061 atcatcaaca ccggatccca ccgcatcaat atagccgcta aattcatggc ctaacgttat
    30121 tggataataa tgtgcaccat ttttaaatat cctgggtaaa tcggaaccac ataagcccga
    30181 gctggcaatt tttacccgca cctcatcctg atgtttaatt tcaggaatga cgctttttgc
    30241 aacgcgcacg ataccatcag tatcattcac tactgatttc ataaaaactc ctgat
//
LOCUS       DADLTY010000031        29797 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.031, whole genome shotgun sequence.
ACCESSION   DADLTY010000031 DADLTY010000000
VERSION     DADLTY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.031"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            56..850
                     /locus_tag="JN334_001165"
     CDS             56..850
                     /locus_tag="JN334_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY7437381.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            872..2281
                     /locus_tag="JN334_001166"
     CDS             872..2281
                     /locus_tag="JN334_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7437382.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2259..3038
                     /locus_tag="JN334_001167"
     CDS             2259..3038
                     /locus_tag="JN334_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAY7437383.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3002..3895
                     /locus_tag="JN334_001168"
     CDS             3002..3895
                     /locus_tag="JN334_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY7437384.1"
                     /translation="MAGLFTPKDAAMNIAIVTINQENAAIASWLAAQDFSGCTLAHWQ
                     IEPQPVVAEQVLDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLD
                     IPTVSMRKSHWGNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPD
                     WLVSTEDLKNVSRAPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGG
                     VDAEKVIGISGHLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIG
                     VVDDWKVVLEALVTNIHAEYQ"
     gene            complement(4043..4618)
                     /locus_tag="JN334_001169"
                     /pseudo
     CDS             complement(4043..4618)
                     /locus_tag="JN334_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4635..4895)
                     /locus_tag="JN334_001170"
     CDS             complement(4635..4895)
                     /locus_tag="JN334_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY7437385.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4886..6157)
                     /locus_tag="JN334_001171"
     CDS             complement(4886..6157)
                     /locus_tag="JN334_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7437386.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6235..6600)
                     /gene="queD"
                     /locus_tag="JN334_001172"
     CDS             complement(6235..6600)
                     /gene="queD"
                     /locus_tag="JN334_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HAY7437387.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            6916..8715
                     /gene="cysJ"
                     /locus_tag="JN334_001173"
     CDS             6916..8715
                     /gene="cysJ"
                     /locus_tag="JN334_001173"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAY7437388.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8715..10427
                     /gene="cysI"
                     /locus_tag="JN334_001174"
     CDS             8715..10427
                     /gene="cysI"
                     /locus_tag="JN334_001174"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HAY7437389.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10501..11235
                     /gene="cysH"
                     /locus_tag="JN334_001175"
     CDS             10501..11235
                     /gene="cysH"
                     /locus_tag="JN334_001175"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY7437390.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11499..11651
                     /locus_tag="JN334_001176"
     CDS             11499..11651
                     /locus_tag="JN334_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7437391.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11845..12996
                     /locus_tag="JN334_001177"
     CDS             11845..12996
                     /locus_tag="JN334_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HAY7437392.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKNRRGEKEGIDDYHLLCWHLLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAARFFAWLLCWHDIGKFARSFQQLYLPP
                     EFKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPRIEIPTFWDDDEGVELIKQLSW
                     YISATVVLADWTGSSTRFFPRVAQAMDIEDYWQKALVQAQNALTVFPPQAETAPFTGI
                     NTLFPFIENTTPLQQKVLDLDINQPGPQLFILEDVTGAGKTEAALILAHRLMAAGNAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPEVFPAPAEINRRNTVRFSRK"
     gene            complement(13082..14119)
                     /gene="iap"
                     /locus_tag="JN334_001178"
     CDS             complement(13082..14119)
                     /gene="iap"
                     /locus_tag="JN334_001178"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HAY7437393.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14371..15279
                     /gene="cysD"
                     /locus_tag="JN334_001179"
     CDS             14371..15279
                     /gene="cysD"
                     /locus_tag="JN334_001179"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HAY7437394.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15281..16708
                     /gene="cysN"
                     /locus_tag="JN334_001180"
     CDS             15281..16708
                     /gene="cysN"
                     /locus_tag="JN334_001180"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HAY7437395.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16708..17313
                     /gene="cysC"
                     /locus_tag="JN334_001181"
     CDS             16708..17313
                     /gene="cysC"
                     /locus_tag="JN334_001181"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HAY7437396.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17363..17686
                     /locus_tag="JN334_001182"
     CDS             17363..17686
                     /locus_tag="JN334_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HAY7437397.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17880..18191
                     /gene="ftsB"
                     /locus_tag="JN334_001183"
     CDS             17880..18191
                     /gene="ftsB"
                     /locus_tag="JN334_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAY7437398.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18210..18920
                     /gene="ispD"
                     /locus_tag="JN334_001184"
     CDS             18210..18920
                     /gene="ispD"
                     /locus_tag="JN334_001184"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAY7437399.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18920..19399
                     /gene="ispF"
                     /locus_tag="JN334_001185"
     CDS             18920..19399
                     /gene="ispF"
                     /locus_tag="JN334_001185"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAY7437400.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19396..20445
                     /gene="truD"
                     /locus_tag="JN334_001186"
     CDS             19396..20445
                     /gene="truD"
                     /locus_tag="JN334_001186"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAY7437401.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20426..21187
                     /gene="surE"
                     /locus_tag="JN334_001187"
     CDS             20426..21187
                     /gene="surE"
                     /locus_tag="JN334_001187"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAY7437402.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21181..21807
                     /gene="pcm"
                     /locus_tag="JN334_001188"
     CDS             21181..21807
                     /gene="pcm"
                     /locus_tag="JN334_001188"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAY7437403.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21947..23086
                     /gene="nlpD"
                     /locus_tag="JN334_001189"
     CDS             21947..23086
                     /gene="nlpD"
                     /locus_tag="JN334_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HAY7437404.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23149..24141
                     /gene="rpoS"
                     /locus_tag="JN334_001190"
     CDS             23149..24141
                     /gene="rpoS"
                     /locus_tag="JN334_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HAY7437405.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24261..24668)
                     /locus_tag="JN334_001191"
     CDS             complement(24261..24668)
                     /locus_tag="JN334_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY7437406.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24815..25408
                     /locus_tag="JN334_001192"
     CDS             24815..25408
                     /locus_tag="JN334_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY7437407.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25408..26355
                     /locus_tag="JN334_001193"
                     /pseudo
     CDS             25408..26355
                     /locus_tag="JN334_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26628..27191)
                     /locus_tag="JN334_001194"
     CDS             complement(26628..27191)
                     /locus_tag="JN334_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage CI repressor"
                     /protein_id="HAY7437408.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27356..27607
                     /locus_tag="JN334_001195"
     CDS             27356..27607
                     /locus_tag="JN334_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7437409.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27609..29705
                     /locus_tag="JN334_001196"
     CDS             27609..29705
                     /locus_tag="JN334_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY7437410.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHNEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7294 a         7385 c         8062 g         7056 t
ORIGIN      
        1 tcgtcacatc atcatcggca acgaaggtgc attgtgctat atcactgaag taacagtgaa
       61 aatctttaaa tacaccccgg aaaacaacct cttctacggc tatatcctgg aagacatgaa
      121 aaccggcttc aacatcctgc gtgaaatcat ggtggaaggg tatcgtccgt cgatcgctcg
      181 tttgtatgac gctgaagatg gcacccaaca cttcacccat tttgccgacg gaaaatgtgt
      241 gctgatcttt atggctgaag gtaaccctcg cattgcgaag gcgacgggcg aagggattgc
      301 ggaaatcgtt gcccgctacc cgcagtgcca gcgcgtggac agcaaactga ttgaaacctg
      361 gtttaacaac ctgaactggg gaccggataa agtggctgcc gaacgtgtgc agatcctcaa
      421 aaccggcaac atgggcttta ccaccgaagt gtccggctgc tggagctgca tccacgaaat
      481 ctacgaaagc gttattaacc gtattcgtac tgagttcccg cacgccgacg acatcaccat
      541 gctgggcggt cactcctctc atagctatca gaacggcacc aacatgtact tcgtctatga
      601 ctacaacgtt gttgactgta agccggaaga ggaaatcgac aagtaccaca atccgctcaa
      661 caaaatcatc tgcgaagaaa ccattcgcct cggcggttcg atggtgcacc accatggcat
      721 tggtaaacat cgcgttcact ggagcaagct ggaacacggc agcgcgtggg cgttgctgga
      781 agggctgaaa aagcagttcg atcctaacgg cattatgaat acgggtacta tctatccgat
      841 tgaaaaataa tgtatcaggc agcgttccgc gatgacgggg cgttgccttt tcggcttctc
      901 aggcgagaag ccgttcttat taccggacaa tgaaggggta aagatgaaca cttcaccggt
      961 gcgaatggat gatttaccgc ttaaccgttt tcactgtcga attgctacgc tcactttcgg
     1021 cgcacacctg accgacggtt atgttctagg cgtcattggt tacgccatta ttcaacttac
     1081 gcccgccatg caactgacgc cgtttatggc gggaatgatc ggcggctcgg cgctccttgg
     1141 tttgtttctt ggcagcctgg ttcttgggtg gatctccgac catattggtc ggcaaaaaat
     1201 cttcaccttc agctttttgc tgattacgct cgcttcgttc ttgcaatttt ttgccaccac
     1261 gccagagcat cttattgggc tgcgcatttt gatcggcatt ggtctgggag gcgattactc
     1321 agtaggtcac accttgctgg ctgaattttc cccgcgccgc catcgcggta ttttgctggg
     1381 cgcattcagc gtggtgtgga ccgtaggcta tgtgttggca agtattgccg gacatcactt
     1441 tatttccgaa aacccggagg cctggcgctg gttgctggca tcggcagctc tgcccgcgtt
     1501 gttgattacg ttattacgct ggggaacgcc agaatcgcca cgttggctac tgtgccaggg
     1561 gcgttttgca gaagctcacg ctatcgtgca tcgctatttt ggtccccatg ttttactggg
     1621 cgatgaagtg gtaacggcga cccataaaca catcaaaacc ttgttctctt cgcgttactg
     1681 gcggcgcacg gcgtttaaca gcgtcttctt tgtctgcctc gtaatcccat ggtttgtgat
     1741 ttatacctgg ctgccaacta tcgcccagac tattggtctg gaagatgcgc tgactgccag
     1801 cctgatgctt aatgcgttgt taattgtggg cgcgctgctg ggattagttc tgacgcacct
     1861 gctggcacat cgcaaatttt tgctgggaag ttttttgctg ctggcggcaa cgctggtagt
     1921 aatggcctgt ttgccttccg gcagttcatt aacgctgctg ctttttgttc tcttcagcac
     1981 caccatttcg gcagtcagta atctggtggg cattttgcct gcggaaagtt ttcctactga
     2041 cattcgctcg ctgggagtcg gttttgccac cgccatgagt cgacttggcg cggcggtaag
     2101 tactggcctg ctgccgtggg tgctggcgca gtggggaatg caagtcacct tattgctcct
     2161 agcgacagtg ttgttggttg gttttgttgt gacctggcta tgggcaccag aaactaaagc
     2221 cctcccgctg gtggcggcgg gaaatgtagg aggtgcgaat gaacattctg ttagcgttta
     2281 aagccgaacc ggatgccgga atgctggcgg aaaaagagtg gcaggcggcg gctcagggta
     2341 atagcggacc ggatgtttcg ctactgcgaa gtttactcgg tgctgatgaa caggccgctg
     2401 ccgcgctgct gttagcgcag agaaaaaacg gtacgccgat gtctttaacc gcgttgagca
     2461 tgggggatga acgggcgttg cactggctgc gctatctcat ggctcttggg tttgaggaag
     2521 ctgtattgct ggagacggcg gcagatctgc gctttgcgcc ggaatttgtc gctcgccata
     2581 ttgccgaatg gcagcgtcag aatccgctgg atctgataat taccggcagc caaagcagcg
     2641 aagggcagaa tgggcaaacg ccatttttgc tggcggaaat gctgggctgg ccctgcttca
     2701 cccaggtgga gcgtttcact ctcgacgcgc tgtttatcac cctcgaacag cgtactgaac
     2761 atgggttgcg ctgttgccgg gttcgcttgc ctgcggtgat tgccgtgcgt cagtgcggtg
     2821 aagtggcctt gccagtaccg ggaatgcgcc agcgaatggc tgcggggaaa gcagaaatta
     2881 tccgtaaaac tgttgctgcc gaaatgccag cgatgcagtg tctgcaactg gcaagagcag
     2941 agcaacgacg cggagcgacg ctgattgatg ggcaaacggt agcagaaaaa gctcaaaagc
     3001 tatggcagga ctatttacgc caaaggatgc agccatgaat atcgcaattg tgacaataaa
     3061 tcaggagaat gccgcaatag cgagctggct ggcggcgcag gatttcagcg gttgtacgct
     3121 ggcgcactgg caaatagaac ctcagcccgt agtggcggaa caggtgctgg atgcgatggt
     3181 ggaacagtgg cagcgaacgc ctgcggacgt tgttctcttt ccgccgggaa cgtttggcga
     3241 tgaactgtcg acccggctgg cctggcgttt acatggtgca agcatctgtc aggtgacttc
     3301 gttagatatt ccaaccgtaa gcatgcgcaa atcccactgg ggaaatgcgt taacggcgac
     3361 gctgcaaaca gaaaaacgcc cgttatgcct ttccctggcg cgtcaggcgg gagcagcgaa
     3421 aaacgccacg ttgccctctg gaatgcagca actgaacatt gtcccaggtg cgcttcccga
     3481 ctggctggtt agcacagagg atctgaaaaa tgttagccgc gccccattag ctgaggccag
     3541 acgggtgctg gtggtgggac agggcggaga ggcagataac caggaaatcg ttatgctggc
     3601 agaaaagctg ggagccgaag tgggctacag tcgggcgcga gtgatgaacg gtggcgttga
     3661 tgctgaaaaa gtgattggta tttccggaca tctgctggca cctgaggtgt gcattgttgt
     3721 tggcgcttcc ggcgcagcag cgttaatggc gggcgtacgc aatagcaagt ttatggtggc
     3781 gattaatcat gacgccagcg cagcggtatt ttcacaggca gatattggcg tggttgatga
     3841 ctggaaagtg gtgctggagg cactggtgac aaacattcac gcagaatacc aataacgtgc
     3901 tgccggatga tcctgcctac ggttctggac tggggacctg taggcctgat aagacgcgtt
     3961 aagcgtcgca tcaggcgctg aatgtcggat gtggcgtgcg gccaacaaat caaaacaaat
     4021 tcaattggtt acgattatcc cgtcaaagca attttttcgc taacgtccag actccggtat
     4081 tcgtggtgga aagcgccacg acacccgcgt taatcgcatt acgcgcatct tcttcatcgc
     4141 ataccagccc gccagcaatc agcggttggc ggattttttc cgtcacccag cccagcactt
     4201 tgggcatgca gcctggcagg atctcaatac aatccggatt cgattgcgca acttacttat
     4261 caatgttgtg aaacgaaatc gaatcaacaa taaacaggtg atgaatacag aaaaaaactt
     4321 ccgctcttgc cgcttttagc atcgatgctt tggtgctgat aattccatcc gcctcggtca
     4381 ccagtttcaa aaactgaatc accacttctt tatttgacgc gccttccagc aaatcaacat
     4441 gaataaaagc atatttcccg gcgttcttaa tctttttaac gatattactg atggtgcaga
     4501 tattgccgta caacacggaa ataaactggc attcagaatc gatggccagt tgcaggctgg
     4561 cattatcttt aacggcagca atcaccggat tctggcggag caggtgtaag aggggcataa
     4621 attatcctta ttatttatcc aaagcggtag gtgatgccga atccggaagg cggatagcgc
     4681 cactgttgta gtgtggattc gtcgcacagc aaacggcagg tgccacactc caggcagccg
     4741 cgatagtcaa tacgtaagtc accttcctgt gtgagagaaa aaagacctac cggacaggcg
     4801 ttaatcaacc gttgtaccat ctgacgctca acggagtcag ccggaacaat gtgcggcgca
     4861 tcagcaacgc gccagagatt acgggctaca gacatcgcag actcctgata acatcgcccg
     4921 ccagatgcca caggccatga cgacgtaaat gatgccagaa taactggcgc agcggtggaa
     4981 caggtttatc accctgatcc cataaatcgc gggaaatatc ctgcattaac gcaggccacg
     5041 cccgatacct gccagggcgt tgcaaaagcg ccggaacatg ctgataacgt tgtagaacat
     5101 cccatagcag gctgcgctct acgttgtgat gataaagcgc aaacagattt tgcggctcgc
     5161 ggtgctggca ggcgcttatt agcgtttgtg ccgccgcctg tgcgccagtc agcgccatat
     5221 ccatgccgcg cacggaaatt ccggtattga cgcaactgcg caacgcatcg cccaccagca
     5281 gccagccgtt accggcgtat tgcaccggca tactgtgcaa gccaccttct ggcaccagat
     5341 gcgcaccata ctccagtgat tccgtgtttt tgataagcgt gcgcactgcc ggatgcgctt
     5401 taaagcgagt cagcagctcg cttgccggaa cacgactttg cgtaagggaa gagagcgggc
     5461 aaacaatccc taacgagagc gtttgttgat tagtataaag aaatgcgccg ccgggtaagt
     5521 catcacagat tccgccgctg aacagcaacg ctgcgccttc gttattctcc agatgaaaac
     5581 gttcttcaat agcggatgtt tccagcgaca gcacttcttt gatccccaac gccatcgctt
     5641 cgccagcagg acgagtcact aacccgtgac gttcagccag gacgctgttg gcaccttctg
     5701 ccagcaccac ataacgggcg cggagaatat cgtcaccaca aataacgcca cagactctgc
     5761 cgttttcttc atacagtgca tccaccgtcg ctccggggat gcattcgaca ccttcttttt
     5821 cggcttcggc aaccagccac ggatcgaacc gtgcacgtaa tacattccag gattcaccgc
     5881 cgggctgtaa gctggaaaac gtcgttgcgc catccggcgt taacagggaa aggctttcgt
     5941 gagtgatgcg tcgttcaaga ggcgcggtca gatgaaattg cgggaggagt tccgcgagtg
     6001 catgggtata taaccgcctg ccggaaagat ttttgctgcc ggggatttca gcgcgttcga
     6061 gtaacaaaac ggataaatcc gctcgcgcgc agcgtaacgc gcaagcggtg cctgcaatac
     6121 cggcaccaat aataataatg tcgcagtcgt cttccatgtc atgccccaga tatccagaac
     6181 acaacaccct aacatagcgt tactaaaggg aaattgaccg ccgacacact cttatcattc
     6241 gccgcgatag atacaccctg cggtgcaggt ttcttttacc atcaccgcac ttaacagcgg
     6301 cacaacgggt ttaacctgat cccaaatcca ttttgctaaa acttcgctgg ttggattttc
     6361 cagacctgga atatcattga gataatggtg atcgaggcgc tcataagttg gcttaaacgc
     6421 cgctttcagt tcagcgaaat cgataatcca gcccgtatgc ggatcgactt ccccggtaat
     6481 ttccagtcgc accataaagg aatgcccgtg caggcgacca catttatgcc cttccgggac
     6541 atgtggtaag cggtgagcgg cttcgaaggt gaaatcttta aataacgtgg tggacatcat
     6601 aatttctcta caggacgata aaaccgccgt agagtaccgg aaaggctgtt ttttcgcatt
     6661 atctaaaacg actttgttgc gcaaaatcgc tgatttatct taatgattgg ctaaattcat
     6721 ttgtttttca ttaggttggt taatctattt tgttgttaaa gactattgct aaaacaggtt
     6781 agtcgatttg gttattagtt atcgctatcc cgtctttaat ccacaccgtt caccccgtta
     6841 accttacctt ctcttctgtt ttatgggcgc tgacagggcg cagaaacagc tttgcttact
     6901 ggaacataac gacgcatgac gacacaggtc ccaccttccg cgttgcttcc gttgaacccg
     6961 gagcaactgg cacgccttca ggcggccacg accgatttaa cgcctaccca gcttgcctgg
     7021 gtttctggct atttctgggg cgtgctcaac cagcagcctg ttgcgcttgc agtgacgcca
     7081 gcgcccgccg cagaaatgcc gggtataacg attatctctg cttcgcaaac cggcaatgcg
     7141 cgccgggttg ctgaagcatt acgcgatgat ttattagcag caaaactgaa cgttaagctg
     7201 gtgaacgcgg gcgactataa attcaaacaa atcgccagcg aaaaactgct catcgtagtg
     7261 acgtcaacgc aaggggaagg ggaaccgccg gaagaagccg tcgcgctgca taagttcctg
     7321 ttctccaaaa aagcgccaaa gctggaaaac accgcgtttg ccgtgtttag cctcggcgat
     7381 agctcttatg aatttttctg ccagtccggg aaagattttg acagcaagct ggcggaactg
     7441 gggggtgaac gcctgctcga ccgtgtcgat tctgatgttg aataccaggc tgctgccagc
     7501 gagtggcgcg cccgcgtggt tgatgcgctt aaatcgcgtg cgcctgtcgc ggcaccttcg
     7561 caatccgtcg ctactggcgc ggtaaatgaa atccacacca gcccgtacag caaagacgcg
     7621 ccgctggtgg ctagcctctc tgttaaccag aaaattactg ggcgtaactc tgaaaaagac
     7681 gttcgccata tcgaaattga cttaggcgac tcgggcctgc gttaccagcc gggtgacgcg
     7741 ctgggcgtct ggtatcagaa cgatccggca ctggtgaaag aacttgtcga actgctgtgg
     7801 ctgacaggcg atgaacctgt cactgtcgag ggcaaaacgt tgcctctgaa cgaagcgcta
     7861 cagtggcact tcgaactgac cgtcaacacc gccaacattg ttgagaatta cgccacgctt
     7921 acccgcagtg aaacactgct gccgctggtg ggcgataaag cgaagttaca gcattacgcc
     7981 gcgacgacgc tgattgttga catggtgcgt ttctccccgg cacagcttga tgccgaagcg
     8041 ctaattaatc tgctgcgccc gctgaccccg cgtctctatt ccattgcctc ctcgcaggcg
     8101 gaagtcgaga acgaagtaca cgtcaccgtt ggtgtggtgc gttacgacgt ggaaggccgc
     8161 gcccgtgccg gtggtgcctc cagcttcctc gctgaccgcg tggaagaaga gggcgaagtc
     8221 cgcgtattta tcgaacataa cgataacttc cgcctgcccg ctaacctgga aaccccggtg
     8281 attatgattg gcccaggcac cggtattgcg ccgttccgcg cctttatgca gcaacgcgcc
     8341 gctgacgaag cgccaggtaa aaactggctg ttctttggca atccgcattt tacggaagac
     8401 ttcctgtatc aggtggagtg gcagcgctac gtcaaagagg gtgtgctgac gcggatcgat
     8461 ctcgcctggt cgcgcgacca aaaagaaaaa gtttacgtac aagataaact gcgcgaacag
     8521 ggcgcggagc tgtggcgctg gatcaatgac ggtgcccaca tttatgtctg cggcgacgct
     8581 aatcgcatgg cgaaagacgt tgagcaggca cttctggaag tgattgccga atttggtggc
     8641 atggacaccg aagcggcgga tgaattttta agtgagctgc gcgtagagcg ccgttatcag
     8701 cgagatgtct actaatgagc gaaaaacatc cagggccttt agtggtcgaa ggaaaactga
     8761 cagacgccga gcgcatgaag cttgaaagca actacctgcg cggcaccatt gcggaagatt
     8821 taaacgacgg tctgaccggc ggctttaagg gcgataactt cctgctgatc cgcttccacg
     8881 gcatgtatca gcaggatgac cgcgacatcc gcgccgaacg tgctgaacag aagctggagc
     8941 cgcgccacgc gatgctgttg cgctgccgtc tgccgggtgg agttatcacc accaaacagt
     9001 ggcaggcgat cgataaattt gccggtgaaa acaccatcta tggcagcatt cgcctgacca
     9061 accgccagac gtttcagttc cacggcattc tgaaaaagaa cgtcaaaccg gtgcaccaga
     9121 tgctgcactc ggtcggtctt gatgcgctgg cgactgctaa cgacatgaac cgtaacgtac
     9181 tctgcacctc gaacccttac gagtcgcagc tgcacgcaga agcgtacgag tgggcgaaga
     9241 agatttctga acatctgctg ccgcgtaccc gcgcgtatgc ggagatctgg ctcgatcagg
     9301 aaaaagtcgc caccaccgat gaagaaccga tccttggtca gacttacctg ccgcgtaagt
     9361 tcaaaaccac ggtagtgatc ccgccgcaga acgatattga tctgcacgcc aacgacatga
     9421 acttcgtggc gatcgcggaa aacggcaagc tggtgggctt caacctgctg gtgggcggtg
     9481 ggctttctat cgaacacggt aacaagaaaa cctacgcccg cacggcgagt gagtttggct
     9541 atctgccgct agagcatacg ctagcggtgg cggaagccgt cgtgacaact cagcgtgact
     9601 ggggtaaccg aaccgatcgt aaaaatgcca aaaccaaata cacactggag cgcgtggggg
     9661 ttgagacgtt taaagcggaa gtggagcgtc gcgcggggat caaatttgaa ccgatccgcc
     9721 cgtatgaatt tacaggacgc ggcgatcgta ttggctgggt taagggcatt gatgataact
     9781 ggcacctgac gctgtttatc gaaaatggtc gcatccttga ttatccgggg cgtccgctga
     9841 aaactggcct gctggagatc gcgaagatcc acaaaggtga tttccgcatt acggcgaacc
     9901 agaatctgat catcgccggt gtgccggaaa gcgagaaagc gaagatcgag aaaattgcca
     9961 aagagagcgg gttaatgaat gccgtcacgc cgcagcgtga aaactcgatg gcctgcgtgt
    10021 cattcccgac ttgcccgctg gcgatggcgg aagcggagcg tttcctgccg tcttttatcg
    10081 acaacatcga taatttaatg gtgaaacatg gtgtcagcga tgagcatatc gtgatgcgtg
    10141 taacaggctg cccgaacggt tgtggtcgcg cgatgctggc ggaagtgggc ctggtgggta
    10201 aagcaccggg tcgctacaac ctgcatcttg gcggcaaccg cattgggaca cgtatcccac
    10261 ggatgtataa agaaaacatc accgagccgg aaatcctggc gtcgcttgat gaactgatag
    10321 ggcgctgggc gaaagagcgc gaagcgggtg aaggcttcgg cgactttacg gtgcgtgcgg
    10381 gcatcattcg cccggtgctc gatccggcgc gcgatttgtg ggattaacca tcagcccggt
    10441 cttgtaggcc tgataagacg cgtgagcgtc gcatcaggca aggcaaacag tgaggaatct
    10501 atgtccaaac tcgatctaaa cgccctgaac gaactgccga aggtagatcg cattctggcg
    10561 ctggcagaaa ctaacgccga actggaaaaa ctggacgctg aaggccgcgt tgcctgggcg
    10621 ctggataatc tgcccggtga atatgtactt tcttccagct ttggcattca ggcggcggtg
    10681 agcctgcatc tggtgaatca gattcacccg gatattccgg tgatcctcac cgacaccggc
    10741 tatctgttcc cggaaaccta ccgctttatt gacgagttaa cggacaaact caagctcaac
    10801 ctgaaagtgt accgcgctac cgaaagcgcc gcctggcagg aagcacgcta cggcaaactg
    10861 tgggaacagg gcgttgaagg cattgaaaag tacaatgaca tcaacaaagt cgaaccgatg
    10921 aaccgggcgc tgaaagaact gaatgcgcaa acgtggtttg ctggcctgcg gcgtgaacaa
    10981 tccggcagcc gtgccaattt accggtgctg gcaattcagc gtggcgtatt taaagtgctg
    11041 ccgattatcg actgggataa ccgaactatt tatcagtacc tgcaaaaaca tggcctgaaa
    11101 tatcacccat tatgggacga aggatattta tcggtgggcg atacccatac aacccgtaaa
    11161 tgggaacccg gcatggcgga agaagaaacg cgtttctttg gcttaaaaag ggaatgtgga
    11221 ttacacgaag ggtaaatatt gccggataaa ttaaacgcct ctgtcagaaa tgatgggggc
    11281 gtttttattt tgcgagcagc attccggcat ttaaaggtga acatcgcagg gaaaattaac
    11341 aggtatagaa tggcgttggg tgcttgaggc tgtctggcgt taagcattcg cgaggttcca
    11401 gatggacaaa agccccaggc gatatttcta tcaacctgag gctcgcgttc gaacgtagca
    11461 attcggatgt tagtctcttc tttgcaggga ggcaagacat gctgacaaaa tatgcccttg
    11521 tggcaatcat cgtactgtgt tgtacagtac tgggattcac gttgatggta ggtgactcgt
    11581 tatgtgagtt gagtatcaga gaacgtggta tggagtttaa ggcagttctc gcttacgaat
    11641 cgaagaagta gccaccacgc gggggtaaaa ccccgcgtac tgaattgtga tggtttggtc
    11701 tcctgcaccc tttttttaaa tttatatttt ttatgtgaat atatagtctc tatttattat
    11761 tatgaatttc aaatgaaaat cattaattta ttcataatgt aaatgtttta ttggctgata
    11821 tatatcatag atagttttta aaatatgcgt aaatatcctt taagtttact gaaggataaa
    11881 aatattgtga ctttctttga tttctgggga aaaaaccgac gtggcgagaa agagggtatc
    11941 gacgactatc accttctttg ctggcatttg ctggatgtgg ccgcaatggg ctatttaatg
    12001 gttaaaagaa attgcttcgg gctggctgat tattttcgtc aattagggat ttctgacaag
    12061 gaacaggcag ctcgattttt cgcctggttg ctgtgctggc acgatattgg aaaatttgcc
    12121 cgctcttttc aacaacttta cctgccccct gaattcaaga ttcaggaagg tgccagaaaa
    12181 aattacgaaa aaatctctca ttcaacgctg ggctactggt tgtggaatca ttatttaagt
    12241 gaatgtcagg agttacttcc ttcatcttcg ctctctccgc gcaaacttag gcgagtgata
    12301 gaaatgtgga tgcccgtgac gacagggcat catggtcggc cccctgatcg tatggatgaa
    12361 ttggataatt ttctgcctga agacaaagct gcagcacgag attttctcct tgaaatcaag
    12421 ccactgtttc cgcgaataga gatccccaca ttctgggatg atgacgaggg cgttgaactt
    12481 ataaaacaac tttcctggta tatctctgca accgtcgtac ttgcagactg gacagggtcg
    12541 tcaacgcgat tttttccacg cgtcgcacag gcaatggata ttgaagatta ctggcaaaag
    12601 gctttagttc aggctcaaaa cgcattaacc gtctttccgc cacaagcaga aaccgcgcct
    12661 ttcaccggaa ttaatacgct gtttcctttt attgagaata cgacaccgtt acagcaaaag
    12721 gtgctggatc tggatatcaa tcagccagga ccacagttgt ttattctgga agatgtgacc
    12781 ggcgcaggta aaacagaagc ggcgcttatc ctggcgcaca ggttgatggc tgcgggtaac
    12841 gctcaggggc tgttttttgg cctgccaaca atggcaacgg ccaatgccat gtacgatcga
    12901 ctggtcaaaa cctggcttgc tttctattcg ccagaggtgt tccccgctcc agcggagata
    12961 aaccggcgca atacggtgcg gttttcccgg aagtaaaccg gacgcactgg atgcgatgat
    13021 gggtatcact tagaattccc cgcccctgcg gtagaactcc cagctcccat tttcaaaccc
    13081 atcaagacgc cttcgccagc tccttcacca acggtagcat tatccgcata acgtcacggc
    13141 agcgacgttc tattcttcca ggaagtgcct tatcaatatg ttgttgatta tccagtctta
    13201 cgtcatgcca gctatttccc gccgggaagg caggtgtttt tgcgcgttgc tgataaccat
    13261 ccttatttcc aagattccag ttagtcgctt ccaccgaaag tacagcaatg cccgctttgt
    13321 cgaatatttc tgcgtcatta caacccccag tgcctttcgg ataattttta ttcaaacccg
    13381 gattggtcgt tgcggctatt ccgtgactgc gtgcaattgc cagcgccctg tcgcgcgtta
    13441 atttccttac tgcttcaggg gtttttacac cgctgttgaa atacaattta tcgccaacaa
    13501 ttaagttatc gagattaatc accagcagcg tatttttctt ttcggtgtca ctcatccgct
    13561 tgagtaaatt ctcagcgcct aattttcctt cctcttcgcc gctggtcgcc acaaatcgaa
    13621 taccatactc ggtaggcgta tttttcaggc gttctgccag ttccagcatg acacctaaac
    13681 ctgcggcgtt atcatctatt ccttgtaacg tcagtccgcc gagattggca tctgcgtcag
    13741 catcgctcag cggggcgtaa gtatccagat gcgccataat gatgatctgc tgcggcgctt
    13801 tgccttcatg agcggcaatc accgtacttc ctgtcacgtt atgccagttc ttgcgattat
    13861 cgcgggcggt ataaatatac cgactattaa atgtccgaat atcactgcga taacccattt
    13921 gctgaaactg ttggcgaata taatcggcag ataacatttc tgctggagtt ccggtcatgc
    13981 gtcccggaaa gaaagtagca atatgtcgtg cctgggtatt agcaaaatcg ccaggtttag
    14041 gtgacgaggc gtgtacgggg agaataaagc atacgccgag cgccagggca gcggtacggt
    14101 ggcgcaatgc ggaaaacata gtgagtcctt aaataccatg caaatttttt taccgccata
    14161 gtatgaaact gccgctgcgc taaaacaatt tcaaatcttc ctaaacgccc gaaatccggt
    14221 gccttaagca ctttttgata ttagctttgc caaatcgtta ttccgttaag gaactactca
    14281 ttctaattgg taatttcatt cgttctctta cgctccctat agtcgaaaca tctgatggca
    14341 agaaaatagc ggtattgcaa aggaacggtt atggctcaaa aacgacttac tcacctgcgg
    14401 caactggagg cggaaagcat ccacattatt cgcgaggtgg cggcagaatt ctcaaatccg
    14461 gtgatgctct actctatcgg taaagattcc agcgtcatgc tgcatctggc gcgcaaggcg
    14521 ttttatccag gtacgctgcc tttcccgttg ctgcatgtcg ataccggctg gaaattccgc
    14581 gagatgtatg agttccgcga tcgtacggcg aaagcctacg gctgcgagct gctggtgcat
    14641 aaaaacccgg aaggcgtggc gatggggatt aatccattcg tgcacggcag cgcgaaacat
    14701 accgatatta tgaaaactga aggcctgaaa caggcgctga acaaatacgg ttttgatgcc
    14761 gccttcggtg gtgcgcgccg tgacgaagag aaatcccgcg ctaaagagcg aatctactct
    14821 ttccgtgacc gcttccatcg ctgggatccg aaaaatcagc gcccggagct gtggcacaac
    14881 tacaacgggc aaattaacaa aggcgaaagc atccgcgtct tcccgctctc taactggacc
    14941 gagcaggata tctggcaata catctggctg gaaaatatcg acattgtgcc gctgtatctc
    15001 gctgcggaac gtccggttct ggaacgcgac ggtatgttga tgatgattga tgacaaccgt
    15061 atcgacctgc aaccgggcga agtgatcaaa aaacggatgg tgcgtttccg tacgctgggc
    15121 tgctggccgc tgaccggtgc ggtggagtca aatgcacaaa cactgccgga aatcatcgaa
    15181 gagatgctgg tttccaccac cagtgaacgt cagggtcgcg tgattgaccg cgaccaggcg
    15241 ggatccatgg agctgaaaaa acgtcagggg tatttttaag atgaacaccg tacttgcaca
    15301 acaaatcgcc aatgaaggcg gcgtcgaagc ctggatgatt gcgcaacaac ataaaagcct
    15361 gctgcgtttt ctgacctgtg gtagcgtcga tgacggcaaa agtaccctga ttggtcgtct
    15421 gctgcacgat acccgccaaa tctacgaaga tcagctctca tcgctgcata acgacagtaa
    15481 gcgtcacggc acccagggcg aaaagctgga tctggctctg ctggtggacg gcctgcaagc
    15541 tgagcgcgaa cagggcatca ctattgatgt ggcctaccgc tatttctcta ccgagaagcg
    15601 taaatttatt atcgccgaca ccccagggca cgagcagtac actcgcaata tggcgactgg
    15661 cgcatcgaca tgtgaactgg cgatcttact gattgatgcc cgtaaaggcg tgctcgatca
    15721 aacccgtcgt cacagtttta tctccacact gttggggatc aaacatctgg tcgtggcgat
    15781 caacaaaatg gatctggtgg attacagtga agagacgttc acccgtattc gtgaagatta
    15841 tctgaccttt gccgggcagc tgccgggtaa tctggatatc cgctttgtgc cgctctccgc
    15901 actggaaggt gacaacgtgg cttcgcaaag tgaaagtatg ccgtggtaca gcggtctgac
    15961 actgctcgaa gtgctggaaa ccgtggagat ccagcgagtg gtggatgccc agccaatgcg
    16021 cttcccggtg cagtacgtta accgcccaaa tctcgatttt cgtggctacg ccggaatgct
    16081 ggcatccggt cgcgtggaag tcgggcaacg tgtaaaagtg ctgccctctg gtgtggaatc
    16141 aaacgtcgcg cggatcgtga cttttgatgg tgatcgcgaa gaagtctttg ccggagaagc
    16201 gatcaccctg gtgctgacgg atgagatcga tatcagccgt ggtgatctgc tgctggcggc
    16261 agacgaagcg ttaccggcgg tgcagagtgc gtcggtggat gtggtatgga tggcggaaca
    16321 gccgctttcc ccgggccaga gttatgacat caaaattgcc ggtaagaaga cgcgtgctcg
    16381 tgttgatggc attcgttatc aggttgatat taataacctt acccaacgcg aagttgaaaa
    16441 cctgccgctg aacggcatcg gcctggtgga tctcactttt gacgagccac tggtgttaga
    16501 tcgctatcag caaaatccgg ttaccggtgg gctgattttt atcgatcgcc tgagcaatgt
    16561 gaccgtgggt gccggtatgg tgcacgagcc agttagccag gcaacagctg cgtcatctga
    16621 attcagtgca ttcgaactgg aattgaatgc cctggttcgt cgccactttc cgcactgggg
    16681 cgcgcgcgat ttgctggggg ataaataatg gcgctgcatg acgaaaacgt cgtctggcat
    16741 agtcatccgg tcactgtgca acaacgcgaa ctacaccacg gtcatcgtgg tgtagtgctg
    16801 tggtttaccg gcctctccgg gtccggtaaa tcaacggtcg ccggggcgct ggaggaggcg
    16861 ttacataaac tcagcgtcag tacgtatctg ctggatggcg acaatgttcg ccacggatta
    16921 tgcagcgatc tcggttttag cgatgccgat cgtaaagaga atatccgtcg cgtcggtgaa
    16981 gtggcgaatt tgatggttga agccggactg gtggtgctga ccgcatttat ctcgccacac
    17041 cgcgccgaac gccagatggt tcgcgaacgc gtaggagaag ggcgctttat tgaagtgttt
    17101 gtcgatacgc cgctggcgat ttgcgaagcc cgcgatccaa aaggcttata taagaaagcg
    17161 cgtgccggtg aactgcgcaa ctttacggga atagattccg tttacgaagc gcctgaatcg
    17221 gcagaaattc atctcaatgg tgaacaatta gtaacaaatt tggtacagca attattagat
    17281 ctgttgagac agaacgatat tatcagatcc tgagacacca ccgggtttcc atgcccggtt
    17341 cgtcaaagta acaggatcaa atatgcgtaa tagccataac attacactaa caaataacga
    17401 cagccttact gaggatgaag aaactacatg gtcactgcct ggtgccgtgg tcggttttat
    17461 ctcctggtta tttgcgctgg cgatgccgat gttgatttat ggctctaaca cgctgttctt
    17521 ctttctctac acctggcctt tctttctggc actgatgcct gtcgcggtag tggtggggat
    17581 tgcgctgcat tcattgatgg acggaaagct acgctacagt attgttttca ccctggtgac
    17641 agtaggcatt atgtttggcg cactgtttat gtggctactg ggctaatttg tactttcttg
    17701 tctcctctgt tcataattca aaccgtaact aataatgaga ttatgttctg cacgccctgg
    17761 gtatacgtaa caatgggcaa atgtggtaca tttgcccgcg ttgtcgcggt atccccaaca
    17821 gaggatgtag agtcgtcttc ggatgcatgg gatgatgatg cagtttttca gggggcagga
    17881 tgggtaaact aacgctgctg ttgctggcta ttctggtctg gctacagtat tcgctgtggt
    17941 tcggtaagaa cggtatacat gactataccc gcgtcaatga tgatgtggcg gcactgcaag
    18001 ctacaaacgc gaaacttaaa gcgcgaaacg atcaactttt tgccgaaatt gacgatctca
    18061 atggcggcca ggaggcgctc gaagagcgtg cgcgtaatga actcagcatg accaggccgg
    18121 gcgaaacttt ttatcgtctg gtgcctgacg cgtcgaagcg cgcacagtct gcggggcaaa
    18181 acaatcgata aatcagcccg ggaattaaca tggcaaccac tcatttggat gtttgcgccg
    18241 tggttccggc ggccggattt ggccgtcgaa tgcaaacgga atgtcctaag caatatctct
    18301 caatcggtaa tcaaaccatt cttgaacact cggtgcatgc gctgctggcg catccccggg
    18361 tgacacgtgt cgtcattgcc ataagtcctg gcgatagccg ttttgcacaa cttcctctgg
    18421 cgaatcatcc gcaaatcacc gttgtagatg gcggtgatga gcgtgccgat tccgtgctgg
    18481 caggtctgaa agccgctggc gatgtgcagt gggtattggt gcatgacgcc gctcgtcctt
    18541 gtttgcatca ggatgacctt gcgcgattgt tggcgttgag cgaaaccagc cgcacgggag
    18601 ggatcctcgc cgcaccagtg cgcgatacta tgaaacgtgc cgaaccgggc aaaaatgcca
    18661 ttgctcatac cgttgatcgc aacggcttat ggcacgcgct gacgccgcaa tttttccctc
    18721 gtgagctgtt acatgactgt ctgacgcgcg ctctaaatga aggcgctacc attaccgacg
    18781 aagcctcggc gctggaatat tgcggatttc atccgcagtt ggtcgaaggc cgcgcggata
    18841 acattaaagt cacgcgcccg gaagatttgg cactggccga gttttacctc acccgaacca
    18901 tccatcagga gaatacataa tgcgaattgg acacggtttt gacgtacacg cctttggcgg
    18961 tgaaggccca attatcattg gtggcgtacg cattccttac gaaaaaggat tgctggcgca
    19021 ttctgatggc gacgtggcgc ttcatgcgtt gaccgatgca ttgcttggcg cggcggcgct
    19081 gggggatatc ggcaaactgt tcccggatac cgatccggca tttaaaggtg ccgacagccg
    19141 cgagctgcta cgcgaagcct ggcgtcgtat tcaggcgaag ggttataccc ttggcaacgt
    19201 cgatgtcact atcatcgctc aggcaccgaa gatgttgccg cacattccac aaatgcgcgt
    19261 gtttattgcc gaagatctcg gctgccatat ggatgatgtt aacgtgaaag ccactactac
    19321 ggaaaaactt ggatttaccg gacgtgggga agggattgcc tgtgaagcgg tggcgctact
    19381 cattaaggca acaaaatgat tgagtttgat aatctcactt acctccacgg taaaccgcaa
    19441 ggcaccgggc tgctgaaagc caatccggaa gactttgtgg tggtggaaga tttgggcttt
    19501 gagcctgatg gtgaaggtga gcatattctg gttagaatcc tcaaaaacgg ctgcaatacc
    19561 cgttttgtgg cggatgcact ggcgaaattc ctgaaaattc attcccgtga agtcagcttc
    19621 gctgggcaaa aagacaaaca tgctgtaacg gaacagtggt tatgtgctcg cgtgccgggc
    19681 aaggaaatgc ccgatctgag cgcctttcaa ctggaaggct gccaggtgct ggagtatgcg
    19741 cggcacaagc gcaagctgcg tttaggggcg ctgaaaggta acgcctttac cctggttctg
    19801 cgcgaagtga gcaatcgcga tgacgttgaa caacgtctga tcgatatttg cgtaaaaggt
    19861 gtaccgaact acttcggtgc ccaacgtttt gggattggcg gtagcaactt gcagggcgcg
    19921 ctgcgctggg cgcaaaccaa tactccggtg cgcgatcgca ataaacggag tttttggttg
    19981 tcggcagccc gcagtgcgtt gtttaatcag attgttgctg agcgcctcaa aaaagcagac
    20041 gttaatcaag ttgttgacgg cgatgcgcta caattagccg gacgtggtag ctggtttgta
    20101 gcaaccaccg aagaactggc ggaattacag cgtcgcgtca acgataaaga gctgatgata
    20161 accgccgcat tgccgggcag tggcgaatgg ggaactcagc gtgaagcgct ggcattcgaa
    20221 caagcagctg tcgccgcaga aactgaatta caagctttac tggtgcgcga aaaagttgaa
    20281 gccgcgcgca gagcgatgct gctgtatccg caacaattaa gctggaactg gtgggatgac
    20341 gtcaccgtag agatccgttt ctggcttccg gcgggtagtt ttgcaaccag cgttgtcagg
    20401 gaacttatca acacaacagg tgattatgcg catattgctg agtaattatg acggggtaca
    20461 tgcacccggt atacaaacgc tggcgaaagc cttgcgtgag tttgctgacg ttcaggtggt
    20521 cgcccccgat cgtaaccgca gcggcgcttc aaattctctg acactggaat cctccctgcg
    20581 cacgtttacc tttgaaaatg gtgatattgc tgtgcaaatg ggaaccccga ccgattgtgt
    20641 ctatcttggc gtgaatgctc tgatgcgtcc gcgcccggac attgttgtgt ccggaattaa
    20701 cgccgggccg aatctggggg atgatgttat ttattccggt acggtagccg ccgcgatgga
    20761 aggccgtcat ttaggttttc cggcgctggc cgtctcgctt gacgggcata aacattacga
    20821 cactgccgcg gcggtaacct gttcaatttt gcgcgcactg tgtaaagagc cgctgcgcac
    20881 cggacgtatt cttaatatta acgttcagga tttacccttg gatcaaatca aaggtattcg
    20941 cgtgacgcgc tgcggtacac gacatccggc agatcaggtg atcccgcagc aagatccgcg
    21001 cggcaatacg ctgtactgga ttggcccgcc gggcggtaaa tgtgatgccg gtccggggac
    21061 cgattttgct gcggtagatg agggctatgt ctccatcacg ccgctgcatg tggatttaac
    21121 tgcgcatagc gcgcaagatg tggtttcaga ctggttaaac agcgtgggag ttggcacgca
    21181 atggtaagca gacgcgtaca agcacttctg gatcaattac gtgcgcaagg tattcaggat
    21241 gagcaggtgc tgaatgcact tgccgccgtg ccgcgtgaaa aattcgttga tgaagcgttt
    21301 gaacaaaaag cctgggacaa tatcgccttg ccgataggtc aggggcagac aatttcgcag
    21361 ccgtatatgg tggcgcgaat gaccgaatta ctcgagctga cgccgcagtc gcgggtgctg
    21421 gaaattggca ctggttcggg atatcaaacg gcaatcctgg cgcatcttgt ccagcatgtt
    21481 tgctcggttg aacggattaa aggcttgcag tggcaggcac gtcgccgcct gaaaaatctt
    21541 gatttacata atgtttcaac ccgtcatggt gatggatggc aaggttggca ggcacgtgcg
    21601 ccgtttgacg ctatcattgt tacggcggca ccgccggaaa ttccaactgc gctaatgacg
    21661 cagctggacg aaggcgggat tctcgtctta cccgtagggg aggagcacca gtatttgaaa
    21721 cgggtgcgtc gtcggggagg cgaatttatt atcgataccg tggaggccgt gcgctttgtc
    21781 cctttagtga agggtgagct ggcttaaaac gtgaggaaat acctggattt ttcctggtta
    21841 ttttgccgta ggtcagcgta tcgtgaacat cttttccagt gttcagtagg gtgccttgca
    21901 cggtaattat gtcactggtt attaaccaat ttttcctggg ggataaatga gcgcgggaag
    21961 cccaaaattc accgttcgcc gcattgcggc tttgtcactg gtttcgctat ggctggcagg
    22021 ctgttctgac acttcaaatc caccggcacc ggtcagctcc gttaatggca atgcgcctgc
    22081 aaatactaat tctggtatgt tgattacgcc gccgccgaaa atggggacga cgtctacagc
    22141 gcagcaaccg caaattcagc cggtacagca gccacaaatt caggctactc aacaaccgca
    22201 aatccagcca gtgcagccag tagctcagca gccggtacag atggaaaacg gacgcatcgt
    22261 ctataaccgt cagtatggga acattccgaa aggcagttat agcggcagta cctataccgt
    22321 gaaaaaaggc gacacacttt tctatatcgc ctggattact ggcaacgatt tccgtgacct
    22381 tgctcagcgc aacaatattc aggcaccata cgcgctgaac gttggtcaga ccttgcaggt
    22441 gggtaatgct tccggtacgc caatcactgg cggaaatgcc attacccagg ccgacgcagc
    22501 agagcaagga gttgtgatca agcctgcaca aaattccacc gttgctgttg cgtcgcaacc
    22561 gacaattacg tattctgagt cttcgggtga acagagtgct aacaaaatgt tgccgaacaa
    22621 caagccaact gcgaccacgg tcacagcgcc tgtaacggta ccaacagcaa gcacaaccga
    22681 gccgactgtc agcagtacat caaccagtac gcctatctcc acctggcgct ggccgactga
    22741 gggcaaagtg atcgaaacct ttggcgcttc tgaggggggc aacaagggga ttgatatcgc
    22801 aggcagcaaa ggacaggcaa ttatcgcgac cgcagatggc cgcgttgttt atgctggtaa
    22861 cgcgctgcgc ggctacggta atctgattat catcaaacat aatgatgatt acctgagtgc
    22921 ctacgcccat aacgacacaa tgctggtccg ggaacaacaa gaagtgaagg cggggcaaaa
    22981 aatagcaacc atgggtagca ccggaaccag ttcaacacgc ttgcattttg aaattcgtta
    23041 caaggggaaa tccgtaaacc cgctgcgtta tttgccgcag cgataaatcg gcggaaccag
    23101 gcttttgctt gaatgttccg tcaagggatc acgggtagga gccaccttat gagtcagaat
    23161 acgctgaaag ttcatgattt aaatgaagat gcggaatttg atgagaacgg agttgaggtt
    23221 tttgacgaaa aggccttagt agaagaggaa cccagtgata acgatttggc cgaagaggaa
    23281 ctgttatcgc agggagccac acagcgtgtg ttggacgcga ctcagcttta ccttggtgag
    23341 attggttatt caccactgtt aacggccgaa gaagaagttt attttgcgcg tcgcgcactg
    23401 cgtggagatg tcgcctctcg ccgccggatg atcgagagta acttgcgtct ggtggtaaaa
    23461 attgcccgcc gttatggcaa tcgtggtctg gcgttgctgg accttatcga agagggcaac
    23521 ttggggctga tccgcgcggt agagaagttt gacccggaac gtggtttccg cttctcaaca
    23581 tacgcaacct ggtggattcg ccagacgatt gaacgggcga ttatgaacca aacccgtact
    23641 attcgtttgc cgattcacat cgtaaaggag ctgaacgttt acctgcgaac cgcacgtgag
    23701 ttgtcccata agctggacca tgaaccaagt gcggaagaga tcgcagagca actggataag
    23761 ccagttgatg acgtcagccg tatgcttcgt cttaacgagc gcattacctc ggtagacacc
    23821 ccgctgggtg gtgattccga aaaagcgttg ctggacatcc tggccgatga aaaagagaac
    23881 ggtccggaag ataccacgca agatgacgat atgaagcaga gcatcgtcaa atggctgttc
    23941 gagctgaacg ccaaacagcg tgaagtactg gcacgtcgat tcggtttgct ggggtatgaa
    24001 gcggcaacac tggaagatgt aggtcgtgaa attggcctca cccgtgaacg tgttcgccag
    24061 attcaggttg aaggcctgcg ccgtttgcgc gaaatcctgc aaacgcaggg gctgaatatc
    24121 gaagcgctgt tccgcaagta agtaagcatc tgtcagaaag gccagtctca agcgaggctg
    24181 gcctttttct tttgtttggg aagcttgtgt ttttgtcgcc tggataagac acgtcagcgt
    24241 cctacccggc aatcagcatt ctatgtgctc atcatcttgc gcaccaactg cataaacagc
    24301 tcctgctctt ctccactcaa acgccccaaa aattcctcat ccacgctgtc accaataggt
    24361 atcgccgcag caagtagctt ttctccttcg gcagtcagcc agacaaagcg tcgccgcttg
    24421 tcagcgacgt catgttcacg tctgactaag ccacgatttt ccattcttgc caacatttct
    24481 gctagcgttg ctttggtgct gactgctgct tctatcagtg ccacctgttc aataccaggt
    24541 ttatcagcaa tggcgcgcat caccgcgtac tgtggtttgg tcaggtcagg taactcgtgc
    24601 tgccagcgag ccgtgtgctg ctgaaaaagc tgtcgtaact gatggaacgc tttatttcgt
    24661 aacgccatgt aaactccggg ctaattctct gtcgctatca tagcggtttt ttagtgacga
    24721 agaagatgtt gttacttttc aatccttgcc gctgtggaaa tgaaggagta tgttaataat
    24781 aacgttcgta tacgaataat taagagaata agcaatgaaa ctgatcgtcg ggatgacagg
    24841 ggctaccggt gcgcctcttg gtgtggcatt actgcaagcg ctgcgtgaga tgccgaatgt
    24901 cgagactcat ctggtgatgt cgaagtgggc gaaaaccacc attgaactgg aaacgcctta
    24961 cagcgctcgc gatgttgctg ccctcgcaga cttcagccat aacccggcgg atcaggcggc
    25021 gatcatctca tccggttctt ttcgtaccga cggcatgatc gttattccgt gcagtatgaa
    25081 aacgctcgcc ggtatccgcg ctggttacgc cgatggcctg gtagggcgcg cggcggacgt
    25141 cgtgctcaaa gaaggccgca aactggtgct ggtgccgcgt gaaatgccgc ttagcaccat
    25201 ccatctcgaa aatatgctcg cactttcacg catgggcgtg gcgatggtgc cgccgatgcc
    25261 tgccttttat aaccatcccg aaacggtaga tgacattgtc caccatgtgg tagcccgcgt
    25321 gctggatcaa tttggcctcg aacatcccca cgccaggcgc tggcaaggat tgccgcaggc
    25381 ccggaatttt tctcaggaga atgaataatg gcatttgatg atttacgcag ctttttacag
    25441 gcgcttgatg accacggcca gttactgaaa atcagcgaag aagtgaacgc tgagccggat
    25501 ctggcagcag cagctaacgc caccgggcgt atcggcgacg gcgcgcccgc gctgtggttt
    25561 gataatattc gcggctttac cgatgcccgc gtggcgatga ataccatcgg ttcctggcag
    25621 aaccacgcga tttcccttgg cctgccacca aacaccccgg tcaaaaagca gattgatgag
    25681 tttatccgcc gctgggataa cttcccgatt gccccggagc gccgcgccaa tccagcctgg
    25741 gcgcagaaca ccgtcgatgg cgaagagatc aacctgttcg atattctgcc gctgtttcgc
    25801 ctgaatgatg gcgatggcgg tttctatctc gacaaagcgt gcgtggtttc ccgcgatccg
    25861 ctcgacccgg ataacttcgg caagcagaac gtcggcatct accgcatgga agtgaagggc
    25921 aagcgtaagc tcggcctgca accggtgccg atgcacgata tcgccctgca tctgcataaa
    25981 gcagaagagc gcggtgaaga tctgccgatt gcgatcacgc tcggtaacga tccgatcatc
    26041 acgctgatgg gggccacgcc gctgaaatac gatcagtctg agtatgaaat ggcaggcgcg
    26101 ctgcgtgaaa gcccgtaccc gatcgccacc gctccgctga ccggttttga tgtgccgtgg
    26161 ggttcagaag tgatcctcga aggggttatc gaaagccgta aacgcgaaat cgaagggccg
    26221 ttcggtgagt ttaccgggca ctactccggt gggcgcaaca tgaccgtggt gcgcatcgat
    26281 aaagtctctt atcgcagcaa accgattttc gaatcactct atctcggtat gccgtggacc
    26341 gaaatcgact acctatgact aggtaattta ttcaagataa ccgggaagag attaaaaaag
    26401 acgggaatag attggaatca acaagttagg aactcaaggt tttcaagata aagcgagtcc
    26461 aaaaatgtta aacggttcga tatcaataaa acattcaaga taactcacag gttcttacaa
    26521 tagcctttaa agaccgttca aaaacgattc aggtgtttat tatagcgctg atgggtgcaa
    26581 gcgccaaaac caatgtaatt ttagcacagc taggcacttg cactgggtta tgcgctggct
    26641 gctttctgag gtagatttaa gtgatcgccc ttagcttcgg acaacgcgta aatctctcta
    26701 accttttcgt ctggcaaatt accaaggctt aacgctatgg ctctacgaac tccaactaga
    26761 gcgtgcaact cttggatgtg ctcatccaga agaatgagta attgctccgc tccttttctc
    26821 ccaagaacat cagctacttg ttgtttttct tgtggtttaa gaaatgacaa gaacattaga
    26881 atttgagcat caacattatc cgcattctct tttttgggtt ctttggaatc ttttagttca
    26941 acatcaccct ctcccgtcat gagccattcc aaggtaacat tttcgctttc tgctatcttt
    27001 tttaactgat acgggcgtgg tatcggtgcg taatcccgcc tgttgtaata attttttaaa
    27061 gtgccttcat tgatccccca tgcccgagca gccgcatttc ttgaagggta tcgttctact
    27121 aacctaaaga ttctttcgat aatcggttct tttccatcgg caggaaaaga accttggggt
    27181 gcttttctca ttcttggttc ctttcgatct aattcattga taaataataa aattaaaaaa
    27241 taaaccataa aaacaccaaa gaaccctttg cagggaatct ttggtatctt aatctttatc
    27301 catacggata tccgaccgga tatccgcgcg ggtaattttg taggttaaag gaaagatgag
    27361 acaaagagaa gatagtgacc gcgattggcc ttcgctgaag attcagctgg cgctcaaaga
    27421 taacgggtgg tcttccttcc gggagctttc actccatgct ggttatgctc aggattcact
    27481 gaaagcggta gttcgcacac caaacaaaaa atacgaacgc gtaatttctg acgctatagg
    27541 tgtaccgcca gaaatcatct ggcccagccg gtatcgtaac gatggattta tcaggaaggc
    27601 gtcgtgatat gcagtcgtac gttaccgtaa atgacctgct cggcgtacca ggaatgccag
    27661 ccaccacaaa aggcatccga caggcgttac agcgtttttc tcgtgatctt ggtgacgtta
    27721 gtcgccgtcg ggaaggcaca aaagccattg agtaccacat tgattgtctg cctgaaataa
    27781 cacgaaaggc actgcgtgaa cgctatgtgg aacagctggt ggctacagag aacaatgttt
    27841 ctgaagtgaa agctgttacc agaaaaacac gcaatcctga cgctgtccag gcaatcgaag
    27901 catatcgcgg ttctccacaa ctgatggaag aacgcctgaa tgcgctgacc gaaaaccagc
    27961 gctgggtatc tgaagcaaga gctgcgctgg tggtggaagt gctgaagctg gaaagcgccg
    28021 gtaaccccgg gcgactgaaa gccattaact ttcttgttga aaaagcccgt aaaggtgagc
    28081 tgccggagcg cctgcaacag gccgcagtta acgccaatgc aaaacgtggc gctaatcgta
    28141 ctatcagccg cgatccactt taccagtggg ttctgaaata caaccagtcg cagaacgccg
    28201 ccgaacggct gctgttactg gcaccgggaa aacgcgacga aataaaaccg gaagaaatca
    28261 gctggctgcc tgaatttctg gcgcagtatc gccaggtgaa cggcagaccc atgtcagaag
    28321 cctacgagga ttttgtcgca gaatggcagc gtcgccatgc ggacgaaccg tacatgctgg
    28381 aggtgatgcc ttcctatgat gttgtccggt atgccatgaa aaaactgccg gaagtggtga
    28441 aacaaaaagg gcgcgttacc ggcagtgaat accgtcagct tgaaggattc acccgccgcg
    28501 actggacagc tatgccggtg aattatgtct ggattggtga cggtcacggt atgaagctga
    28561 aatgtgcgca tccgatccac gggcgtccat tctcaccgga agtcacgttt gtgatcgatg
    28621 gtggaacacg gtttgttgtt ggctggagtc ttgaccttgc cgaaaacgtg ttcgccgttg
    28681 ccggtgcgat acagcacggc attcgcaacc acggaaaacc gttcctgtat tactcggata
    28741 acggttccgg tgaaacggct gacatgctgg acaaagaggt cgtgggtatt ctgccacgcc
    28801 tggggattaa acaccctacc ggtattgccg gtaatccaca gggacgcggc attattgaac
    28861 gcctgaaccg gacactgccg atgcgtatag ccaggaggta tcgcacctat tttggcaagg
    28921 gtgctgaccg ggaatcgttg cgtgtactta accgtgatct gcgttcagcg tttaacgccc
    28981 tgcaacagga caagcctctg aacgaccgcc agaaagcggc aatgcgcgag ctgccatcgt
    29041 gggcggagct tattgaggct attcgtgaag gcgtggaatg gtacaacaac cgcccgcatt
    29101 ctgagctgcc gatgaaaccg aacggacggc attacagccc gacagagttc agaaaaaaac
    29161 gccaggcaga agaagacacc gaaattgagt ggctgagtga tcttgagctg cgcgacatgt
    29221 tccgcccaat ggtggaacga ccggtaagac gctgcgaaat tcagtggctg aacaatattt
    29281 actacgcgcc ggagttgcgt gacgaacacg gtcgcaaagt gcttatcagt tatgacattc
    29341 atgacgccga gcgaattacg gtacgtcgca aggacggcag ctttatctgt gaggcgatat
    29401 ggaacggcaa taaacgcgca gcctttgctg tcagtgctga atatcacaaa caacagcagc
    29461 gtattaaagg aatgcgcaaa cgcgctgagg aaaaaattcg tgatgctgaa gatgaaggta
    29521 ttcagattct ggaacacaaa caggctgaac cctggctgag taatgtttat cgcccggttg
    29581 gtaatgtggt ggcagttcag caaccggaat atgaagaaga gcataacgaa gaatttgaac
    29641 gcgatttccg gctggggatg caaaaacttt ttgccatgca ggaagaagat gatccgctgg
    29701 cctgaataaa aaaacagtcc gcgggaacgg actgttaaat aaaggaaaca aagctgattt
    29761 gaaataaccg aggaaatact atgactgata ttgatga
//
LOCUS       DADLTY010000032        29467 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.032, whole genome shotgun sequence.
ACCESSION   DADLTY010000032 DADLTY010000000
VERSION     DADLTY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.032"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            71..352
                     /locus_tag="JN334_001197"
     CDS             71..352
                     /locus_tag="JN334_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY7437411.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            318..710
                     /locus_tag="JN334_001198"
     CDS             318..710
                     /locus_tag="JN334_001198"
                     /inference="COORDINATES: protein motif:HMM:NF020572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437412.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGVDSFTMAGWIERYKTILIRRKASRNTYKTRVN
                     QLETIKEKLGGVLLTEITTRHIAEFLDL"
     gene            1029..1395
                     /locus_tag="JN334_001199"
                     /pseudo
     CDS             1029..1395
                     /locus_tag="JN334_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1788..2129)
                     /locus_tag="JN334_001200"
     CDS             complement(1788..2129)
                     /locus_tag="JN334_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HAY7437413.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2142..3014)
                     /gene="copD"
                     /locus_tag="JN334_001201"
                     /pseudo
     CDS             complement(2142..3014)
                     /gene="copD"
                     /locus_tag="JN334_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3018..3392)
                     /gene="yobA"
                     /locus_tag="JN334_001202"
     CDS             complement(3018..3392)
                     /gene="yobA"
                     /locus_tag="JN334_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HAY7437414.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3531..3761
                     /locus_tag="JN334_001203"
     CDS             3531..3761
                     /locus_tag="JN334_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAY7437415.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3863..4519
                     /locus_tag="JN334_001204"
                     /pseudo
     CDS             3863..4519
                     /locus_tag="JN334_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            4543..5205
                     /gene="exoX"
                     /locus_tag="JN334_001205"
     CDS             4543..5205
                     /gene="exoX"
                     /locus_tag="JN334_001205"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HAY7437416.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5202..7262)
                     /gene="ptrB"
                     /locus_tag="JN334_001206"
     CDS             complement(5202..7262)
                     /gene="ptrB"
                     /locus_tag="JN334_001206"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HAY7437417.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7472..8131)
                     /locus_tag="JN334_001207"
     CDS             complement(7472..8131)
                     /locus_tag="JN334_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HAY7437418.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8458..8814)
                     /gene="yebF"
                     /locus_tag="JN334_001208"
     CDS             complement(8458..8814)
                     /gene="yebF"
                     /locus_tag="JN334_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HAY7437419.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            complement(8881..9171)
                     /gene="yebG"
                     /locus_tag="JN334_001209"
                     /pseudo
     CDS             complement(8881..9171)
                     /gene="yebG"
                     /locus_tag="JN334_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9305..10483
                     /gene="purT"
                     /locus_tag="JN334_001210"
     CDS             9305..10483
                     /gene="purT"
                     /locus_tag="JN334_001210"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HAY7437420.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10539..11180)
                     /locus_tag="JN334_001211"
     CDS             complement(10539..11180)
                     /locus_tag="JN334_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAY7437421.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11217..13028)
                     /gene="edd"
                     /locus_tag="JN334_001212"
     CDS             complement(11217..13028)
                     /gene="edd"
                     /locus_tag="JN334_001212"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAY7437422.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13263..14738)
                     /gene="zwf"
                     /locus_tag="JN334_001213"
     CDS             complement(13263..14738)
                     /gene="zwf"
                     /locus_tag="JN334_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAY7437423.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15076..15945
                     /locus_tag="JN334_001214"
     CDS             15076..15945
                     /locus_tag="JN334_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY7437424.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16073..17515
                     /gene="pyk"
                     /locus_tag="JN334_001215"
     CDS             16073..17515
                     /gene="pyk"
                     /locus_tag="JN334_001215"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAY7437425.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17646..18617)
                     /gene="lpxM"
                     /locus_tag="JN334_001216"
                     /gene_synonym="msbB"
     CDS             complement(17646..18617)
                     /gene="lpxM"
                     /locus_tag="JN334_001216"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7437426.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18737..20059)
                     /gene="mepM"
                     /locus_tag="JN334_001217"
     CDS             complement(18737..20059)
                     /gene="mepM"
                     /locus_tag="JN334_001217"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HAY7437427.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(20075..21007)
                     /gene="znuA"
                     /locus_tag="JN334_001218"
     CDS             complement(20075..21007)
                     /gene="znuA"
                     /locus_tag="JN334_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HAY7437428.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21086..21841
                     /gene="znuC"
                     /locus_tag="JN334_001219"
     CDS             21086..21841
                     /gene="znuC"
                     /locus_tag="JN334_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HAY7437429.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21838..22623
                     /gene="znuB"
                     /locus_tag="JN334_001220"
     CDS             21838..22623
                     /gene="znuB"
                     /locus_tag="JN334_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HAY7437430.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22770..23780)
                     /gene="ruvB"
                     /locus_tag="JN334_001221"
     CDS             complement(22770..23780)
                     /gene="ruvB"
                     /locus_tag="JN334_001221"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAY7437431.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23789..24400)
                     /gene="ruvA"
                     /locus_tag="JN334_001222"
     CDS             complement(23789..24400)
                     /gene="ruvA"
                     /locus_tag="JN334_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAY7437432.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            complement(24539..24604)
                     /locus_tag="JN334_001223"
     CDS             complement(24539..24604)
                     /locus_tag="JN334_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437433.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24674..25276
                     /locus_tag="JN334_001224"
     CDS             24674..25276
                     /locus_tag="JN334_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HAY7437434.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(25278..25799)
                     /gene="ruvC"
                     /locus_tag="JN334_001225"
     CDS             complement(25278..25799)
                     /gene="ruvC"
                     /locus_tag="JN334_001225"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAY7437435.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(25834..26574)
                     /locus_tag="JN334_001226"
     CDS             complement(25834..26574)
                     /locus_tag="JN334_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7437436.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(26603..27055)
                     /gene="nudB"
                     /locus_tag="JN334_001227"
     CDS             complement(26603..27055)
                     /gene="nudB"
                     /locus_tag="JN334_001227"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HAY7437437.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(27173..28945)
                     /gene="aspS"
                     /locus_tag="JN334_001228"
     CDS             complement(27173..28945)
                     /gene="aspS"
                     /locus_tag="JN334_001228"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAY7437438.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            29255..>29467
                     /locus_tag="JN334_001229"
     CDS             29255..>29467
                     /locus_tag="JN334_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HAY7437439.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
BASE COUNT         7227 a         7568 c         7235 g         7437 t
ORIGIN      
        1 tattaaaaat caaccacacc cgccagtatt ctgtatattt actggcggtc atatcgtgag
       61 aggtatggca atgaatcttg tgacactcaa aacgtgggga aaactcagat atccggataa
      121 cccaccatca atatcaacgc tgagacgatg ggcaaggaat ggaaacattt atcctgcacc
      181 tgaactacac gggaggagtt acagggtggt tccggaggct ttctatatca acccaaataa
      241 ggttgatacc gatataacac accatcagcc taatgggcga caagggagag acagtccgtt
      301 aatggagaag ttaaaacatg cagcggaaaa aatacgatcc caatttgcct agaaacttaa
      361 catatcgaag gagagacaaa gcatattact ggcgcaaccc tctgacgaaa gaagaattta
      421 cactaggtaa aatttcaaga agagatgcag tagcgcaggc aattgaagca aatcattata
      481 tatacaaaaa ctactctcct gctgccctaa ttgaaaagct taaaggggtc gactcattta
      541 ctatggcagg ctggattgaa cgttacaaaa cgattcttat aaggagaaaa gcgtccagaa
      601 atacttataa aactcgggta aatcaactgg agacaataaa agaaaaatta ggaggggttt
      661 tactgacaga aataaccact cgccatatcg ccgagtttct tgatttgtag attgaaggag
      721 ggaaaaacac gatggcagga tcaatgcgtt ctgtgttatc tgatatgttc cgcgaagcca
      781 ttgttgaagg acgtatatct caaaatccag taacgccaac aagagcaccg aaaatagtag
      841 ttacaagaga acggctgaaa ctaaagacat acaactgcat cagggaggca gcagatcaac
      901 ttccggcatg gttcccatta gctatggact tagcccttgt aacaggacaa cgtcgcgaag
      961 acataacgaa tatgcggttc agtgatattt atgatgatcg tctccacatc aggcaaatta
     1021 agacaggaat gatgattgct atccccctgt cactcagcct tcctgtcgct ggtttacgac
     1081 ttggtgcagt agttgaacag tgccgcatgg taagtagggg ggattatcta atcagtgccg
     1141 ggattagaaa aaacagccct gacggcagca ttcacccgga cggcctgaca aagaaatttg
     1201 tcgcagccag aaaattaaca ggtatcactg ttcagtgaaa acccaccaac ttttcacgaa
     1261 atcagaagcc tggctggacg attgtacaaa gaaatatgtg gagaagaatt tgctcagcgt
     1321 ctacttggcc acacatcgga gaagacaaca aaaatgtatc ttgatgagag agaaaaaacg
     1381 tacttactgc tctgatttta acgtaaatgg attgttaaat gtattttggt tgtgatataa
     1441 ccaaaaaaga ccggaataca gaaattcgag taaatttcgg ggaatttcgg ggagacgttt
     1501 gcaactaatt gattttaaat acaattaaaa aaagaccgaa tacgattcct gtattcggtc
     1561 cagggaaatg gctcttggga gagagccgtg cgctaaaagt tggcattaat gcaggcttag
     1621 ttgccttgcc ctttaagaat agatgacgac gccaggtttt ccagtttgcg tgcaaaatgg
     1681 tcaataaaaa gcgcggtgct catcagctta aatgttaaaa accgcccgtt ctggtgaaag
     1741 aactgaggcg gtttttttat tggaaatcaa aaggctattt taggtaatta acagagtttt
     1801 tcagctcgtt ctataaacgg tgccagactc attttttcgc cgggattgtt aggatcatca
     1861 atctgaatca ccgaaatggg ttgggcattg gtcttcccac tggcaacttc cttttgtgcg
     1921 atatcgttta aaggatactg cacgagggta cttgggttga tgacatacaa agcattaccc
     1981 ggtcggcaag tcagcattac ctcttcgcga ttaaacgccc atttgtcttt acccacttca
     2041 aaacgactca cggttatcac ctgtggagcc gccagcgccg ctgcagaact ggtgagtaac
     2101 agaaacgcca gaatactttt tttcatcatt ttcgtaatcc atcaaaaagg ttcgagagtc
     2161 gcaagcaggc tgatgatcac cagcactacc gcacctatcg cccattcgag tttagtcatc
     2221 cacacaaacc agggtgccgc acgatcttca tcctgcctca tgcgcggtac aagaacataa
     2281 cgattagcca gcgcaatgac caccataatc atcaccagga tggctttgag caataagagt
     2341 tggccccagt aagtcgtaag cgtgggggga aatccggtaa tcaacaatgc attaagcacg
     2401 ccgcttgcca gtacgccgat caccgcaaaa tgcccgcacc aggaaaaacg catcagcgcc
     2461 tgaatagcct agtgtcgcca acggcctttg atgagttgca tacaccagag caccggcagt
     2521 aatcccccaa accacgccgc cgcacaaatc aggtgaatcg cgtgattagt ctgatggatt
     2581 ttcgctgtta ccccttcatt cagcgtcgca taccccactc ccgccagcag aataaattgc
     2641 gcggtagtga gcataaacag caaacgtggc atattccgcg gttgcataag ggcgacgatc
     2701 aacgttacga gggcgagaac aatttgccat aaccagacgc caccaaactg cgtttgcaaa
     2761 acagcctgcc aaatgtttgg cgaaaataca tctgtccatc ccgttcccat caacccgccc
     2821 tgaacggcga gcattgcagt ggcactgatc aaactccaga cggcagcgtg ctgttgtaaa
     2881 cgcagagaac gcttcgtcag caagcgacga atcattaagg gggccagcca ggcaccatac
     2941 attgcaaagc caaagaccag catcaacgag gtgaaatgga taaatcgcag cgcgatccag
     3001 gtaaacgcca gcatgattta tttcacgcta aaggtgtagt gtcctttcgt tttatgcccg
     3061 tccaccgaaa caacatgcca gtcaacggtg taggttccgg gtttcagcga atcggccagt
     3121 ggaacaatca gttgcttttg gtcctgctca ttccgcttcg ctggtaatgt tttaatattt
     3181 tcgttttttg gccccgtgat ttttgcacca ctgaatcctg tttcaacgcc ttccgagaag
     3241 tttaaagtga ttgcctgcgg ggcagctgtc acttgcgcgt ttgccgcagg atactgatgc
     3301 gttaaatgcg catgtgccca gacggaaggt gtcaccaacg aagtggtcag gattgccagc
     3361 gcgtagcgaa gggagcgtgc agttgaagcc atattatcta ttcctttttg taataacttt
     3421 tttacagagc ataaccttgt ctaatgtctg agtcgaggat catcaattcc ggcttgccat
     3481 ccaggctcac tcttagtaac ttttgcccgc gaatgatgag gagattaaga atgctgaaga
     3541 atctggctaa actggatcaa acagaaatgg ataaagtgaa tgtcgatttg gcggcggccg
     3601 gggtggcttt taaagaacgc tacaatatgc cggtgatcgc tgaagcggtt gaacgtgaac
     3661 agccagaaca tttgcgcagc tggtttcgcg agcggcttat tgcccaccgt ttggcttcgg
     3721 tcaatctgtc acgtttacct tacgagccca aacttaaata aaacttatac agagttacac
     3781 tttcttacat aacgcctgct aaattatgag tattttctaa accgccccca taatttgcag
     3841 tcattttgaa aaggaagtca ttatgtcgtt ctggaaagtt gcagcagcgc aatatgagcc
     3901 tcgtaaaacc tcactcaccg aacaggtagc tcatcatctg gagtttgtga gggccgccgc
     3961 caggcaacaa tgtcagctgt tagtttttcc atcgctttct ttactggggt gtgattattc
     4021 gcgacgtgcc cttcctgccc cacccgatct ttcactgtta gacccgcttt gttatgccgc
     4081 aacgacctag cgaatgacca tcattgccgg ccttcccgtt gaatataacg atcgctttat
     4141 tcgtggaatt gcggtgttcg caccgtggcg aaaaacgcct gggaactacc atcagagtca
     4201 tggtgcatgt ctgggccgtc gttccagaac catcacggtg gtcgatgaac aaccgcaagg
     4261 tatggatatg gacccgacct gttcactgtt tacgacaggg caatgtctgg gagaacccga
     4321 cctgctggcg tcggcccgtc gcttacaatt tttttcacat cagtactcga ttgccgtgct
     4381 gatggcaaat gcccgtggta acagtgcact atgggatgaa tatggtcgtc tcatcgttcg
     4441 cgccgatcgt ggttcattat tgttagtcgg tcagcgttca tcacagggtt ggcaaggcga
     4501 tatcattcca ttacgctagg ctttttcggc ctggatcatg ccatgttgcg cattatcgat
     4561 acagaaacct gcggtttgca gggagggatc gttgagattg cctctgttga tgtcattgac
     4621 ggaaaaatcg tcaaccccat gagcctcctg gtgcgccccg atcgtcctat tagtccacaa
     4681 gcgatggcga ttcatcgcat caccgaagcc atggtcgccg ataaaccgtg gattgaagat
     4741 gtgatcccac actattacgg tagtgaatgg tatgtcgcgc acaacgccag ctttgaccgc
     4801 cgcgtactgc ctgagatgcc cggtgagtgg atttgcacta tgaaactggc ccgtcgtttg
     4861 tggccagggc tcaagtacag caatatggcg ttatataaaa cacgcaagct caatgtacag
     4921 acaccgctgg gcctgcatca tcaccgcgcg ttgtatgact gttatatcac cgccgcgttg
     4981 cttatcgata ttatgaacac ctccggctgg acggcagaac agatggccga tatcaccgga
     5041 cgtccgtcgt tgatgacaac cttcaccttt ggcaaatacc gtggcaaagc ggtttccgac
     5101 gttgccgaac gctatccggg ctatctgcgc tggttattta ataacctgga cagcatgagc
     5161 ccggagctgc gtttaacact gaaacattat ctggaaaata cttaatccgc aggcgtagcg
     5221 ggtaatgttc cctgcgccag cgcgaccaga aaagcatatt ccatcgctac gccttcgtac
     5281 gatttaaagc ggccagattt accgccatgg cctgagtcca tgtcggtaca gagcaataaa
     5341 agatggtcat cggttttcag ctcgcgcaat ttagcgaccc attttgccgg ttcccaatat
     5401 tgcacctgag aatcgtgtaa accggtcgtt accagtaaat gcggataagc ctgtgcggtg
     5461 acgttgtcat acgggctgta gcttttcatg tactcgtaat attgcggatc ctgcggattt
     5521 ccccactctt caaactcacc agtggtaaga ggaattgatt catcaagcat cgttgttaca
     5581 acatcaacaa acggtacctg ggcgataacg ccgtggaata attccgggcg ttgattaatt
     5641 gcaacgccca tcaacatgcc ccccgcactt ccgcccatcg cataacagag cgaaggagag
     5701 ccatagccca gttttaacaa tgcatcgcag gcatcaagat aatcattaaa cgtatttttc
     5761 ttcttcagaa atttaccgtc ttcgtaccat tgttgcccca gctcaccacc gccgcgaaca
     5821 tggacaatgg cgtagacaaa gccacgatct aacaaactca agcggctaaa actgaaatcg
     5881 gcatcaatac ttgcgccgta agaaccatag ccatacacca gcaacgggtt gtgtcctttg
     5941 cgaaaatgtt tgcgatgata gaccagcgaa accggaactt cgacgccatc acgggcgact
     6001 atccacaggt gttcactgcg gtaattcgcc gcatcaaaac caggcacttc tgtttgtttt
     6061 aatacacgac gctcaccggt atccatatcc agttcaaaca aagtgtctgg tgtggtcatg
     6121 gaagaataac cataacgcaa tcgcgcggtt tcaggttctg gattgtaggc aatccaggtc
     6181 acataggccg gatcatcaaa ggcaatgcca atgacttccc gggtcttgcg gttaatttgg
     6241 cgcaaactgg ttaacccgcg ctgacgctct tcaaccacca gccagtcggt aaacagcgta
     6301 aacccttcca gcatgatgtt ttcgcgtggc ggaattaact cttcccactg ttgctcatca
     6361 cgcatacggg tacggtataa gccaaagttt ttgccgtggc gattggaacg cagataaaaa
     6421 cgatgctggt agtgatcaag gctgtattcg tgatctttgc ggcgcggcag aaaaacaaac
     6481 ggctcggcat cggccatttc cgcgtccagc aggcgaactt cactggtggt ggcgctggcc
     6541 agatgaatga cgacatagtg cttcgacgtc gttttatgca ggctgacata ataggtatcg
     6601 tctttttctt cgtagatcag tgtatcttgc gatgctggcg taccgatggc gtgacgccag
     6661 acctgataag gcagcagcgt caccggatgc ttgcgaacat agtagaaagt ccaggagtca
     6721 tttgcccaga caaagctggg ttcaacgtta tccagcagtt ccgggtacca gttacctgtt
     6781 tccagattac gaaaacgaat gccgtactgg cgtcgggaaa gaaaatcttc tgccagcgcc
     6841 ataatggtgt tatcgggcgt aatcgccatt ccgcccatcg aataaaactc actatgagct
     6901 gcgcgcttgt tggcatcgag caatgtttcc cactcatccc actcttcgct gaatgccgat
     6961 tgacgctggt agatagcata ttcacagcct ggttcataaa tatgccgata gcggtagcca
     7021 ttttttatgt agggcgcaga aacttctctt tgcggaatgc ggtcgatgat ttcctttaag
     7081 atgcgatcct gcaaggcttg ttgtgaggcc atcacccgat gaccgtaact attttcttgt
     7141 tgcaggtagt ccaggacttt tggctgagaa cgcgtatcgt cccgcagcca gtagtaatta
     7201 tcgatgcgcg tatcgccatg aagcgtcatg gcgtggggaa tgcgggcggc ttttggtagc
     7261 atgttattgt tctttctggt tggaaacatc ttataagggt ggcaaaactc accggggatg
     7321 caagcgaaac agggtcgtca ttgcttagat gatgacaggt aatggcgcgg atatcgaatg
     7381 ttatgcagac agagaaaatc agcctgttca aatggctgtg cggttctgga tagcccgaaa
     7441 tagtcaactt caggctatcc agagagcgga attattccgc caaagtgcgt ttttgctgtt
     7501 cgagatcgcg ttcaatgcct tcacgaacat cctgggggat tttcagcgcg tcacccagtg
     7561 cattcaggta actgcgttcc ataaaatggt caatatcaat agccgcgcag ctcaggaaat
     7621 agatttccag cgcctcttct tcattgcgga ccccggtagc caggcgttgt ggatccagcg
     7681 gttgttcgat tgcctgctca atgagtacac gcccctgctc ttccacgccg gcttcacgca
     7741 attgctggtc gattgccgca cgttcattgg catcaatatg accatcactt ttagcggcaa
     7801 agaccagcgc aaggatcaaa cgttccgtac gctcatccag cggcgtactt tgcgcgccaa
     7861 actgcggttc gtcctgatgc gccgcgcgaa ttttatcttt gtatttattc cacagcaccg
     7921 tacccgctac cgctccgccg ccaaccagta acgcgttggt gccatatttc gtaagaagtt
     7981 tacgtgctga tttatttgcg accagcagcc cagccagtcc gcctaatgcg cctgacacta
     8041 acagtttgcc caatccctga tccgcagacg aggaggtaga agaactgctt tgcccaagca
     8101 gggattgcag ttgatttaac cagttagcca tatttgctcc tcaataacca tttttagtgc
     8161 ctgtagcata gcggatgagg atgtcagaaa atgtatatcg agacaaaaga tgcgcaaata
     8221 agcggggcgg taggccgtta ttcaaagaaa ttcgcgccag gtaaggtatt tactgtatat
     8281 caggtgataa gggtatttta ggtgaaagta gtttgtcgtt ttgagtggca ttgtttgatg
     8341 ctgattgccc gatgcgacgc tgttgcgtct tctcaggcct acgattgtgg tgcgttctgg
     8401 gacggacaag gcactcgcgc cgcatccgcc aacggtcaat cgaattatca gaagccatta
     8461 acgccgctga tattccgcca ttcccgcttt gcagtccacg cagacctggt aatgaatatc
     8521 ggcactttta ccacgcacgg ttagcggtac tgaccattta tcatctttac cctgaatgtc
     8581 ctgcaaactg acccaggcca cgggatcggc ctgaccgaca attttttgat catctgccca
     8641 gcgcgccacg cgattttgtt gataatcacg ttttacgctc gcggcaattc cggcagcatc
     8701 cagaccttca cactttggga aagtgaccga cttgctggtt tcattattgg cagcgaaaac
     8761 tgatgcgcag gcagaaacca acaaccgccc taaaaacgcc cctctttttt tcatgttttt
     8821 ctccatagca caatgattca ggagaaagca tggtacaaat tgtcaggagc gcaagttgct
     8881 tcaggccgcg tgtgaggcat cttcctcttc ggattcagca ccgacgacct gtggggacgg
     8941 taatttgccg gttttcagaa tggtgctcag cacctctttt tgttctgcca gccatagcga
     9001 aagggcttca cgttgctcgt cttccatttg cactggagaa tttgtcagct aggtgtcaag
     9061 cagatccgcc gtatcaagca ttttgtcata agcatcggct tcctttttgc tggtaaacga
     9121 cattttctct tcgccctcac gaatgactac gtatttaact tcaaccgcca tttgcagcct
     9181 ctcataataa ctgagatttt atacagtata tttcttttcg gttgagaaat caacatcagc
     9241 aataaagaca cacgcaaacg ttttcgttta tactgcgcgc ggaattaatc aggggatatt
     9301 cgttatgacg ttattaggca ctgcgctgcg tccggcagca actcgcgtga tgttattagg
     9361 ctccggtgaa ctgggtaaag aagtggcaat cgagtgtcag cgtctcggcg tagaggtgat
     9421 tgccgtcgat cgctatgccg acgcaccagc catgcatgtc gcgcatcgct cccatgtcat
     9481 taatatgctt gatggtgatg cattacgccg tgtggttgaa ctggaaaaac cacattatat
     9541 cgtgccggag atcgaagcta ttgccaccga tatgctgatc caacttgaag aggaaggact
     9601 gaatgttgtc ccctgcgctc gcgcaacgaa attaacgatg aatcgcgagg gtatccgtcg
     9661 cctggcggca gaagagctgc agctgcccac ttccacttat cgttttgccg atagcaaaag
     9721 ccttttccgc gaggcggttg ccgccattgg ctatccctgc attgtaaaac cggtgatgag
     9781 ctcttccggc aaggggcaga cgtttattcg ctctgcagag caacttgctc aggcatggga
     9841 atacgctcag caaggcggtc gcgccggagc gggccgcgta attgttgaag gcgtcgttaa
     9901 gtttgacttc gaaattaccc tgctaaccgt cagcgcggtg gatggcgtcc atttctgtgc
     9961 accagtaggt catcgccagg aagatggcga ctaccgtgaa tcctggcaac cacagcaaat
    10021 gagcccgctt gcccttgaac gtgcgcagga gattgcccgt aaagtggtgc tggcactggg
    10081 cggttatggt ctgtttggtg tcgagctgtt tgtctgtggt gatgaggtga ttttcagtga
    10141 ggtctcccct cgtccacatg ataccgggat ggtgacgtta atttctcaag atctctcaga
    10201 gtttgccctg catgtacgtg ccttcctcgg acttcctgtt ggcgggatcc gtcagtatgg
    10261 tcctgcagct tctgccgtta ttctgccaca actgaccagt cagaatgtca cgtttgataa
    10321 tgtgcagaat gccgtaggcg cagatttgca gattcgttta tttggtaagc cggaaattga
    10381 tggcagccgt catctggggg tggtactggc tacttcagag agtgttgttg acgccattga
    10441 acgcgcgaag cacgccgccg gacaggtaaa agtacagggt taaacccggc aaaaaaacgc
    10501 tacaaaaatg cccgatccta gatcgggcat tttgactttt acagcttagc gccttctaca
    10561 gcttcacgcg ccagcttagt aatgcggtcg taatcgcccg cttccagcgc atctgccgga
    10621 accagccagg aaccaccgat gcacagcacg cttttcagtg ccaggtagtc acggtagtta
    10681 gccggagaaa taccacccgt cgggcagaaa cggacctggg agaacggacc cgcgatcgcc
    10741 tgcagggctt tcacgccgcc gttagcttca gccgggaaga atttgaactc tttcaaaccg
    10801 tagtccatac ccagcatcag ttcggaaaca gtgctgatcc ccggaatcag aggaatagtc
    10861 ccttcggtag cagctttcag cagcggctcg gtcagacccg ggctaattgc gaactgtgca
    10921 cccgcttcag tgacttctgc cagctgctgt ggattcagca ccgtaccggc acccacaatc
    10981 gcttcaggca cttctttggc gatagcacgg atagcgtcaa ctgcacactc ggtacgcaga
    11041 gtcacttcca gaacgcgcac cccaccagca accaacgctt ttgccatcgg caccgcgtgt
    11101 tccagttttt ttaccacgat aaccggtaca accgggccgg tggtcaggat tgattctgca
    11161 cttgttttcc agtttttcat cagagttttc tctcgcctga ttacaaattt gtcgtcttaa
    11221 aaagtgatac aggttgcgcc ctgttcggca ccggacagtt tttcacgcaa ggcgctgaat
    11281 aattcacgtc ctgttcccac gcgtgacgcg ctcaggtcag gaatgtgcgg ttcgcgagca
    11341 gccagttccg cttcgtctac cagcagtgtc agttcgcctg tctgtccatt cacacgaatg
    11401 atgtccccgt cgcgcacttt tgccagcagc ccgccatcgt aggcttctgg tgttacgtgg
    11461 atagctgacg gcactttacc tgaagcgccg gagagtcgtc catcggtaac taacgcaatt
    11521 ttgaaacacc ggtccaataa tacaccaagt ggcggcatga gtttatgtaa ttctggcatt
    11581 ccgttcgctt ttggcccctg atgacggaca acaacgacac aatcgcggtc cagcaaaccc
    11641 gcttcaaagg ccggcataac gtcatgctgg ctttcaaaaa caaccgctgg cgcttcaatc
    11701 acctggttct caaccggcac ggcagaggtt ttcataaccg cacggcccag gttaccgctt
    11761 aacacttttg tcccaccatg atgagagaaa ggttgttcga aggaagcgat cacattgctg
    11821 tcgagtgatt tttccgcccc ttcccgccag tccagttcac cattattcag ccatggttca
    11881 agggtataac gagacagacc aaagcctgcc accgtattga catcttcatg cagcaggcct
    11941 gctttgagca gttcacgcac cagaaccggt acgccacctg ccgcctggaa gtggttaata
    12001 tcggccggac cgttcgggta gagacgtgcc atcagcggta caacatcaga aaggtcagag
    12061 aagtcatccc agttaatctg aataccggcc gcgcgcgcca tcgccaccag gtgcatggtg
    12121 tggttagtgg aaccaccggt cgccagcagt gcaacgatac cgttcaccac cactttctca
    12181 tcgatcatct taccgatcgg catccattca ttaccattac cggtcatgcg tgtaacctga
    12241 cgcgcagcgg cagcggtcaa agcatcgcgc agcggagaat ccggatgaac aaaagaagag
    12301 cctggcaact gcatccccat aaattccacc accatctggt tggtgttggc agtaccgtag
    12361 aaagtacatg ttcccggcgc atggtaagac gcggcttctg actccagtaa ggccatgcgg
    12421 tccactttac cttcggcata aagctggcga atacgcactt tttctttatt tggcaaaccg
    12481 cttgccatcg gtccagacgg cacaaacacc gcaggcaaat gaccaaacga cagggctgcc
    12541 atcgtcagac ccgggacaat cttgtcgcac acaccgagga acagagcacc atcaaacatg
    12601 ttatgggaca gccccaccgc cgcagacatc gctatcactt cgcggcttag cagcgacaat
    12661 tccattccat cctgcccctg ggtgacacca tcacacatcg ccggaacacc gcccgcaacc
    12721 tgaccaaccg cattcgcttc atgcagggct ttacgaatga tttctggata gtgttcataa
    12781 ggctggtgcg cggagagcat gtcgttatag gaggtgatga tggcgatatt gttacgcaac
    12841 atgcttttca aagaggcttt gttttctggc tggcaggcag cgaaaccgtg tgccaggtta
    12901 ccgcatgcca actgcgaacg atgaacggtc gacgttttcg cttgttctat ccgggcgaga
    12961 taagcagagc gagtctcgcg cgaacgttca atgattcgat ttgttacgcg taacaattgt
    13021 ggattcataa aggctcctga aattgagttg tcagagcagg atgattcaca acgcgtttca
    13081 ttcagaggat ttatgagtga aacgcctgta accggagctc atagggcaaa aacgtttcag
    13141 tcagtgtaat aaaaaaagcc tcgtgggtga atccgcacga ggcctgaaag tgtaaaaatt
    13201 gttctacaat ctgtgcaaga tcatgttacc ggtaaaataa ccataaagga taagcgctga
    13261 tattactcaa actcattcca ggaacgacca tcacgggtaa tcatcgccac cgaggcaacg
    13321 ggtccccagg ttccggcctg atacggtttc ggcgcatcat tgtccatcgc ccacgcctca
    13381 gtaatggagt ctacccattt ccaggcttct tccacttcgt cgcgacgtac aaacagtgcc
    13441 tgaataccac gcatggtttc cagcagcaaa cgttcatagg catccgccag atgcgtctga
    13501 ttaaaggttt ctgaatagct cagatccagc ttggtgattt gcaggttatg tttgtggtca
    13561 aggccaggaa ctttattcag tacctggata tccacgcctt catcaggttg cagacggata
    13621 gtcagtttat tctgcggcag atcctgccac gactctttaa acagattcag ttcaggtgtt
    13681 ttgaaataga ccacgacttc agaacatttg gtcggcagac gtttaccagt acgcaggtag
    13741 aatggcacac cggcccagcg ccagttatca atgtcgacgc ggatcgccac gaaggtttct
    13801 gtattgctgc ttttgttcgc gccctcttct tccagatatc ccggcacttt tttgccctgg
    13861 gcgaagcccg cagtatattg cccgcgtacg gttttttcgc gtacgttgga gcggtcgatg
    13921 cggcgcaggg acttcagtac tttcactttt tcatcgcgga tgctgtctgc gctcaggtca
    13981 gatggcggag acatcgcaat catgcagaga atttgtagca ggtggttctg aatcatgtca
    14041 cgcatctgac cggctttatc aaaatagccc cagcgccctt cgatccccac ttcttctgcc
    14101 acggtaatct caacatgatc aatggtgcga ttgtcccagt tattcacaaa cagggagtta
    14161 gcaaaacgca gcgccaacag gttcagcacc gtttctttac caagatagtg gtcgatacgg
    14221 taaacctggc actcctcgaa gtattcgcca acctgatcat tgatttcctg cgaggtcgcc
    14281 agcgacgtcc ccagcggttt ctccatgact acgcgtgccg gtttagcatt cagttttgcc
    14341 tcgccaagcc ctttgcaaat tgcgccaaaa gtgctgggcg gcatggcaaa gtagttaata
    14401 gtgatacgat ttttttgatc cagcatcgcg ccgagacggt tgaatgcagc agtgtcattg
    14461 acatcgagat tacaaaaatc cagacgtgca ctcagggtgt cccataaacc ttcatcaatg
    14521 gtttctttca tgaaagtttc gagcgcctcg cggacaactt tggtatatgc cgctttatcc
    14581 cagtcagcac gccctacgcc gataatccgg gtgtccgggt tgagctgacc gactttttcc
    14641 agttgataca gggaaggcag caatttacga cgcgcaaggt cgcctttcgc gccgaaaatg
    14701 accaggtcac aggcctgggc tgtttgcgtt accgccatgt cattctcctt aagttaacta
    14761 acccggtact taagccaggg tatacttgta attttcttac ggtgcactgt actgctttta
    14821 cgagcttgcg aaaactgtaa acgcttatcc acccgtgcga ttacgggaaa agcgcgcaaa
    14881 gcgcggcaaa actgataaaa aaatcatcgt tttccattcg gtaaaaatct gacactgatc
    14941 atgttatgaa aaaaaataac aactttttta tctgcttttg tcattaacac ggcacacagg
    15001 cgtaatatct gacaaaactg catttcgatt tctttcagtg cggaaatcgt cattacccgt
    15061 gagtctcttt acatcatgaa tatgctggaa aaaatccagt ctcagctgga acatttgagc
    15121 aaatcagagc gcaaagttgc cgaggtcatt ctggcttcgc ccgataacgc gatccattcg
    15181 agtattgctg ctctggcgct ggaagccaat gttagcgaac cgacggtgaa tcgtttctgt
    15241 cgcagcatgg acacgcgcgg ttttcctgat tttaaacttc atctggcaca aagtctggcg
    15301 aatggcactc cctatgttaa tcgcaacgtc aatgaagatg acagcgttga atcatacacg
    15361 gggaaaatat ttgagtccgc aatggcaacg ctcgatcatg tccgtcattc actggataaa
    15421 tctgccatca accgcgccgt cgacttgctc actcaggcaa aaaaaatcgc ctttttcgga
    15481 ttaggctctt ctgccgccgt tgcccacgat gcgatgaata agttctttcg ttttaatgtt
    15541 ccggtggtgt actccgatga tatcgtgctg caacgcatga gttgtatgaa ttgtagcgac
    15601 ggagacgtgg tggtgctgat ttctcacaca ggaagaacaa aaaatctggt cgagctggcg
    15661 cagctggcac gcgaaaacga cgccatggtg attgccctca cctctgcggg taccccgctc
    15721 gcccgggaag caacgctggc aattaccctc gacgtaccgg aagatactga catttatatg
    15781 cccatggttt ctcgacttgc acagctgacc gtgatagatg tgctggcgac aggatttact
    15841 ttgcgacgcg gtgcaaaatt cagagataac ttgaagcggg tcaaggaagc gctgaaggaa
    15901 tcgcgttttg ataagcagtt acttaattta agtgacgatc gctaaaagcg actgtcactg
    15961 tcctaatctt atacgacatc cgaatgagat taatttatcg ccatcgcggc gttatttcat
    16021 tcggatttca tgttcaagca acacctggtt gtttcagtca acggagtatt acatgtccaa
    16081 aaggcttcgc agaacaaaaa tcgttaccac gttaggccca gcaacagatc gcgataataa
    16141 tcttgaaaaa gttatcgcgg cgggtgccaa cgttgtacgt atgaactttt ctcacggctc
    16201 gcctgaagat cacaaaatgc gcgcggataa agttcgtgag attgccgcaa aactggggcg
    16261 tcatgtggct attctgggtg acctccaggg gcccaaaatc cgtgtatcca cctttaaaga
    16321 aggcaaagtt ttcctcaata ttggggataa attcctgctc gacgccaacc tgggtaaagg
    16381 tgaaggcgac aaagaaaaag tcggtatcga ctacaaaggc ctgcctactg acgtcgtgcc
    16441 tggtgacatc ctgctgctgg acgatggtcg cgtccagtta aaagtactgg aagttcaggg
    16501 catgaaagtg ttcaccgaag tcaccgtcgg tggtcccctc tccaacaata aaggtatcaa
    16561 caaacttggc ggcggtttgt cggctgaagc gctgaccgaa aaagacaaag cagacattaa
    16621 gactgcggcg ttgattggcg tagattacct ggctgtctcc ttcccacgct gtggcgaaga
    16681 tctgaactat gcccgtcgcc tggcacgcga tgcaggatgt gatgcgaaaa ttgttgccaa
    16741 ggttgaacgt gcggaagccg tttgcagcca ggatgcaatg gatgacatca tcctcgcctc
    16801 tgacgtggta atggttgcac gtggcgacct cggtgtggaa attggcgacc cggaactggt
    16861 cggcattcag aaagcgttga tccgtcgtgc gcgtcagcta aaccgagcgg taatcacggc
    16921 gacccagatg atggagtcaa tgattactaa cccgatgccg acgcgtgcag aagtcatgga
    16981 cgtagcaaac gccgttctgg atggtactga cgctgtgatg ctgtctgcag aaactgccgc
    17041 tgggcagtat ccgtcagaaa ctgttgcagc catggcgcgc gtttgcctgg gtgcggaaaa
    17101 aatcccgagc atcaacgttt ctaaacaccg tctggacgtt cagttcgaca atgtggaaga
    17161 agctattgcc atgtcggcaa tgtacgcagc taaccacctg aagggcgtta cggcgatcat
    17221 taccatgacc gaatcgggtc gtaccgcgct gatgacctcc cgtatcagct ctggtctacc
    17281 aattttcgcc atgtcgcgcc atgaacgtac gctgaacctg actgctctct atcgtggcgt
    17341 tacgccggtg ccctttgata gcgctaatga cggcgtagca gctgccagcg aagcggttaa
    17401 tctgctgcgc gataaaggtt acttgatgtc tggtgacctg gtgattgtca cccaaggcga
    17461 cgtgatgagt accgtgggtt ctactaatac cacgcgtatt ttaacggtag agtaagtacg
    17521 ttgccggatg cggcgaaaac gccacatccg gcctatagtt caatgatagt tcaacagatt
    17581 tcgaatattc tgaagcaaac ttgaacttat catcaggcga aggcctctcc tcgcgagagg
    17641 cttttttatt tgatgggata aagatctttg cgcttatacg gctggatttc gcccggtttg
    17701 cgagttttca gcaattttag tatccaggtg tattgttctg gtcgcggacc aacaaaaatc
    17761 tcgacttctt cattcatccg ccgctcaatc gtatgatcat ccgcctctaa cagatcatcc
    17821 atcggtgggc gcacctgaat cgtcagacga tgcgtcttgc catcataaat cggaaacagc
    17881 ggtacaacgc gcgcacggca tactttcatc aaacgaccaa tcgcgggcaa cgtcgcttta
    17941 taggtggcaa agaaatccac aaattcgctg tgctctgggc catgatcctg atcgggtaaa
    18001 taatatcccc agtacccctg acgtaccgac tggatgaatg gtttaatacc gtcatttctc
    18061 gcatgcagac gaccgccaaa gcgacgacgc accgtgttcc agacataatc aaaaaccggg
    18121 ttgccctgat tatggaacat cgctgccatt ttctgccctt gcgaggccat cagcatggca
    18181 ggaatatcga cggcccaacc gtgcggcacc agaaagataa ctttctcgtt attacgccgc
    18241 atctcttcga tgatctccag cccttgccag ccaacgcgcg gctgaatttt ctccggcccg
    18301 cgtattgcca actcagccat cattgccatc gcttgcggcg cggtggcaaa catctcatca
    18361 acaatcgctt cgcgttcagc ttcactacgt tctggaaagc agagcgacag attgattaac
    18421 gcacgacggc gtgagctttt tcccagtcgt ccggcaaaac gtcccagccg tgccagaatg
    18481 ggatcacgga actttggcgg cgttaaagcg atacccgcca tcgctgctac acccagccat
    18541 gctccccagt agcgcgggtg gcgaaaggat ttatcaaact caggaatgta ttcgctatta
    18601 ttttttttcg tttccatgct tttccagttt cggataaggc aaaaatcaat ctggtgatag
    18661 tgtagcggca caacttgccc cgcaccaaat aaaaaaagcc ggtactgact gcgtaccggc
    18721 tgcgaatgga tgttaattaa tcaaaccgta gctgcggcac aatctctttg gcctgcgcca
    18781 ggaattcgcg acgatcggag ccggtcagcc cttcggtacg cggcagtttt gccgtcagcg
    18841 ggtttacggc ctgctggttt atccatactt catagtgcag atgcggccca gttgaacgtc
    18901 cggtattacc ggaaagcgcg atacggtcgc cacgtttcac cttctgtccc ggtttcacca
    18961 gaatcttgcg caagtgcata taacgcgtgg tgtagctgcg accatgacga atagccacat
    19021 aataacctgc tgcgccactg cgtttggcaa ccaccacttc accgtcaccc actgaaagca
    19081 ctggcgtacc ttgtggcatg gcgaaatcaa cacctctgtg tggtgcaacg cgacctgtca
    19141 ccggattagt tcgacgcggg ttaaagttag acgagatacg gaactgtttc gccgtcggga
    19201 atcgcaagaa tcctttcgcc agaccagtac cgttacgatc gtagaatttg ccatcctcag
    19261 cgcggattgc gtaataatct ttaccttctg aacgcaaacg tacgcccagc agctggcttt
    19321 gctcacgttt accatcaagc atttctcgag acattaatac cgcaaattca tcgccttttt
    19381 tcagtttgcg gaaatccatt tgccactgca tggctttaat cactgcgctc acttcggcgc
    19441 tggttaaacc ggcgtttctg gcgctggcaa caaagctccc tccgacggta cctttcagca
    19501 gattgttaac ccactctcct tgctgcattt cgctggtcat tttaaaaccg ttagcggcag
    19561 tacggtcata ggttcgggtt tcacgacgag acacttccca ggtgaggcgc tgcagttcgc
    19621 cgtccgcggt taatgtccag gagagttgtt gaccgatttt caggttacgc aattctttgt
    19681 cggcagcagc aagttgggtg atatcaccca tatcaatacc atactgattg agaatgctgc
    19741 ttagcgtatc gccagtggaa acaacatatt catgcacgcc cgcttcaccg gcgattttgt
    19801 catccagttc gtcctgggga atggcttcat cttcttgtgc agcttgatca atcggctcac
    19861 tggcttcagg taagagcgaa cgaatttcgt tctgttccag ctcaatggtt ttgacaattg
    19921 gcgtggcatc acggtgataa acatagggcc gccagacagc gacggccaga gtaagaacgg
    19981 tgagcgaccc caacataacg cggtgtggtc gcggtaaatt attaaacgcc agggcgacag
    20041 agcgggctat ctgttgcacg taatcacttc ctcattaatc tcctttcagg cagctcgcat
    20101 actggttggc taattgactc aggaattctg aatagcttgt tttacccagt ttgatattcg
    20161 tccccagggg atccaacgtt cccatacgaa cggatgtccc tcgtgcgacg ctctcaacga
    20221 ccgctggcct gaactgtggc tcagcaaaaa cgcaggttgc tttttgctca accaactgtg
    20281 ttcttatttc atgtaaacgc tgcgcgccag gttgaatctc agggttaacg gtaaaatgac
    20341 caagcggtgt cagtccgaac tgtttttcga aatagccgta agcatcgtga aaaacgaaat
    20401 aacctttccc cttgagcggc gcgagctcgt taccaacctg cgtttcggtt gaggccaatt
    20461 gtgcctcaaa atccttcagg ttggcgtcaa gtttggctcg actttgcggc ataagttcca
    20521 ctaattttcc atggattgca accgctgtag cccgcgctat ctctggggaa agccaaagat
    20581 gcatgttgaa atcgccgtga tggtgatctt cgtcactttt ttccgcgtgg tcgtgatcat
    20641 catcatcgcc gtgaatactt ttcatcagta gcggtttcac atcttcaagc tgcgcaatcg
    20701 ttacctgctt cgcttctggt aatttgctta ccggtttttg cataaacgct tccatctccg
    20761 ggccaaccca aacgactaag tccgcgttct gtaagcgttt tacatccgat ggacgcagtg
    20821 aataatcatg ttctgaagcg ccgtcaggca gtaaaacctc cgtttctgtt acaccatcag
    20881 caatggcaga agcgatgaac ccaacgggtt taagcgaagc gacaacggcg gcatctgcgg
    20941 cctgtgttgc acctccccag agagcggcgg ataatgctgc gaaaagaagc gtttttttat
    21001 gtaacataat gcgaccaatc atcgtaatga atatgagaag tgtgatatta taacatttca
    21061 tgactactgc aagactaaaa ttaacatgac aagtctggtt tccctggaaa atgtctcggt
    21121 ttcttttggc caacgccgcg tcctctctga tgtgtcgctg gaacttaaac ctggaaaaat
    21181 tttgacttta cttgggccaa atggcgcagg taagtcgaca ctggtacggg tagtgctcgg
    21241 gctggtaaca cccgatgaag gggttatcaa gcgcaacgga aaactgcgca tcggctatgt
    21301 accgcagaag ctgtatctcg acaccacgtt gccactgacc gtaaaccgtt ttttacgctt
    21361 acgccctggt acacataaag aagatatttt gcctgcactg aaacgtgtcc aggccgggca
    21421 cctgattaac gcaccgatgc aaaagctctc tggtggcgaa acgcagcgtg tactattagc
    21481 gcgagcattg ttaaattgcc cgcaattatt agtgctggat gaacccactc aaggcgtgga
    21541 tgtaaatggc caagtggcgt tatatgacct tattgaccag ttgcgtcgtg aactggattg
    21601 tggcgtttta atggtttctc acgatctgca tctggtgatg gcaaaaaccg atgaagtgct
    21661 gtgcctgaat caccacattt gttgttccgg cacaccggaa gttgtttccc tgcatccgga
    21721 gtttatttca atgtttggtc ctcgtggtgc tgaacaactg ggtatctatc gccatcatca
    21781 taatcatcgt cacgatttac agggacgaat tgttttgcgt cggggaaatg atcgctcatg
    21841 attgaattat tatttcccgg ttggttagcc gggatcatgc tcgcctgtgc cgcgggtccg
    21901 ctgggttcgt ttgtagtctg gcgtcgtatg tcttatttcg gtgatacgct ggctcatgcc
    21961 tcattacttg gtgtcgcgtt tggtttgttg ctggacgtga atccattcta tgcggtgatt
    22021 gccgttacgc tgctgctggc gggcggtctg gtatggctgg agaagcgtcc acagctggcg
    22081 atcgacacgt tattagggat tatggcgcac agtgccctgt cgctgggcct agtggtcgtt
    22141 agtctgatgt ctaacattcg tgttgatttg atggcttacc tgttcggtga tttactggca
    22201 gtgacgccag aagatctcat ctctattgcg attggcgtgg tcatcgtggt ggctattttg
    22261 ttctggcaat ggcgcaattt gctgtcgatg acgattagcc cggatctggc gtttgttgat
    22321 ggtgtgaaat tacagcaagt gaaattgttg ttgatgctgg tgacggcatt gacgattggt
    22381 gtagcgatga aatttgtcgg tgcgttgatt attacttcgt tgctgattat tcctgcagct
    22441 actgcgcgtc gctttgcccg tacgccggaa cagatggctg gtgtcgctgt tttggtgggg
    22501 atggtggcag taactggcgg tttaaccttt tccgcgtttt acgatacgcc ggcgggtccg
    22561 tcggtggtgc tatgtgcggc actgttattt attatcagta tgatgaaaaa gcaggccagc
    22621 taatctatcg ctgaacacat ttgtcggatg cggcgcgagc gccttatccg acctacggtt
    22681 cgctatctct ggctggcctg ataagacgcg aacagtgtcg catcagacac actgccagtg
    22741 tcggatgcgg cgcgagcgac caatccgact tacggcattt ctggcggcgt tatgccaaag
    22801 tgattccacg cccgcgtcgt cgccatacgc ccgcgcggtg tacgctgcaa aaagccttgc
    22861 tgaatcaaat aaggttccag tacatcttca atggtttcac gttcttcgcc aatggctgcc
    22921 gccaggttat ccagacctac cggcccacca aagaacttat cgattaccgc cagcaacaat
    22981 ttgcggtcca tataatcgaa accttcagca tcgacattca acatatccag cgcctgagca
    23041 gcgatatctg ccgagatggt gccatcgtgc ttcacttcgg cgaaatcacg cactcgacgc
    23101 agcagacggt tggcaatacg tggcgtaccg cgagcgcgac gagctacttc ctgcgcaccg
    23161 tcatcactca tctcaagccc cataaagcgt gcactgcgac tgacgatata ttgcagatcc
    23221 ggcacctgat aaaactccag acgttgcaca ataccaaaac ggtcgcgcaa cggtgatgtc
    23281 agcgaacctg cgcgcgtggt tgcgccaatc agggtaaacg gcggcaaatc aattttaatg
    23341 gagcgtgccg ccggaccttc accaatcatg atatccagtt ggtagtcttc cattgccggg
    23401 tacagcactt cttcaacaac tggcgataaa cggtggatct catcaataaa cagcacgtca
    23461 tgcggttcaa ggttagtgag catcgcagcc aaatcgcccg ccttttccag caccggacca
    23521 gaagtcgtgc gtaaattaac gcccatttca ttggcgacaa tgttggcaag cgtagtttta
    23581 cccaaccccg gaggaccaaa aatcaacaaa tgatcgaggg catcgccgcg cagtttcgct
    23641 gctttgatga aaatctccat ctgtgaacga acctgcggct gaccaacata ctcttccagt
    23701 aatttagggc gaatggcgcg atctgctaca tcttccggca aagtggtacc ggcagaaatc
    23761 agacggtctg cttcaatcat cctttacctc ataacgcggc gcgtagggct tcgcgaatta
    23821 atgtttcact gctggtgtca gggcgagcga ttttgctcac catgcggctt gcttcttgtg
    23881 gtttatagcc tagcgccacc agcgcggcaa ctgcttcttg ttcagcatcg tcggtcgccg
    23941 ggctagcagg agacgtgagt accaggtcgg cggctggcgt aaagagatcg ccatgcaaac
    24001 ctttaaatcg gtctttcatt tcaacaatca agcgttcggc ggtttttttg ccaatacccg
    24061 gcagtttcac cagtgccccc acttcttcac gctcaacggc attaacgaac tgctgcgctg
    24121 acattccgga gaggatcgcc agggccaact tcgggccgac gccgttggtt ttgatcaact
    24181 ctttgaacaa cgtgcgttct tgtttattgt taaaaccgta cagcagttgc gcgtcttcac
    24241 gcaccacaaa gtgggtgaaa acgatcgctt cctgacccgc ttcagggagt tcataaaaac
    24301 aggtcatcgg catatgcact tcatagccta cgccgcccac ttcaattaac accagcgggg
    24361 gttgtttttc aatgatgatg cctctgagtc tgcctatcac atgacgctcc tgcgtaatga
    24421 atcaaagata atgctgtatg ataaaaaaat gctggataga tatccagcga aggatgaaga
    24481 aaacttgcga ggtgtcccga tgatctgaaa aatggcacag tataatttat tcaacaggtt
    24541 atattggaag caaatatttg aatattacat attcagtgaa gaaatgcgta ataaaaatat
    24601 acattgcgac tcctgaaaaa taattttttt atgctattac gtatatttgt atctatttca
    24661 atggaatgac aacgtgaata ttaattaccc tgctgaatat gaaattggag atatcgtctt
    24721 tacatgtata ggtgctgcct tatttggtca aatatcagct gcatcaaatt gctggagtaa
    24781 tcacgtcggg atcattatcg gtcataacgg tgaagacttc ctggttgcag aaagccgggt
    24841 tcccctttca accatcacca cgctatctcg ttttattaaa cgttcgtcta atcaacgcta
    24901 tgctataaag cgattagacg ctgggctgac agaacaacaa aaacaacgaa ttgttgaaca
    24961 ggttccctcc cggctacgca aactttacca cacgggtttt aaatacgaat cttcgcgcca
    25021 gttctgttca aaatttgtct ttgatattta taaagaggcg ctatgtattc cggtgggtga
    25081 aatagagacg tttggacaac tgttaaatag caatccgaat gcaaaactca ctttctggaa
    25141 attctggttc ttaggttcta ttccgtggga gcgtaaaacc gtcacgccag ccagtttgtg
    25201 gcatcatccg ggtttggtgt tgattcacgc ggagggagtt gaaacgcctc agccggaact
    25261 gaccgaggcg gtataactta acgcagtcgc cctctcgtca ggttcagccg cgattcgctc
    25321 atctgcatcg cattctgact aacgtggcag tgggtgatgg caatcgccag cgcatcggcg
    25381 gcatccgcct gtggattagc gggcagtttc agcaaggtgc ggaccatatg ctgcacctgg
    25441 cttttttcgg cactacccat acctaccacc gtttgcttta cctgacgtgc cgcgtattca
    25501 aataccggca attcctgatt caccgccgca acaatcgcca cgccgcgcgc ctggcccagt
    25561 ttcagggctg agtcagcgtt cttcgccata aagacttgtt caatggcgaa ataatcaggc
    25621 tggaactggg tgatgatttc cgtcacgccc gcatagatga gcttcagacg agacggtaaa
    25681 tcatccactt tggtgcgtat gcatccgcta cccaggtagg acagttgcct acctacctgg
    25741 cggatgacgc cgtagccggt cacgcgcgaa cccgggtcaa tgccgagaat aatagccatc
    25801 acgcgtctcc gttttgctgt ttagcaggca tcatcagaga gtcgctgcaa cctcatcaga
    25861 gatttcaccg ttatggtaaa cttcctgcac gtcgtcgcaa tcttccagca tatcgatcag
    25921 acgcatcagt ttcggcgcgg tttctgcatc catatcagct ttggtggacg ggatcatgga
    25981 aacttccgcg ctgtctgctt tcagacctgc cgcttccaga gcgtcgcgca ctttacccat
    26041 ttcttcccat gcagtgtaga catcaatcgc gccgtcatca tatgtcacaa cgtcttcagc
    26101 acctgcttcc agagctgctt ccatgatggt gtcttcgtcg cctttctcga aggagatcac
    26161 gccttttttg ctgaacagat aagctacgga accgtcagtg ccgaggttac cgccacattt
    26221 gctgaatgcg tgacgcactt cagcaacggt acggttgcgg ttgtcagaca gacattcaat
    26281 catgattgcc gtgccgccag gaccgtaacc ttcgtagatg atggtttcca tgtttgcatc
    26341 atcatcaccg cccacaccac gtgcaattgc gcggttcagt gtgtcacggg tcatgttgtt
    26401 agacagtgct ttatccaccg ccgcacgcag acgcgggtta gcgtccggat cgccaccgcc
    26461 cagcttagcc gcggttacca gctcacgaat gattttagtg aagattttac cgcgcttagc
    26521 atcctgcgca gctttacgat gtctggtgtt ggcccattta ctatgacctg ccataaaaat
    26581 atctccagat agccctgcct gttcaggcag cgttaattac aaactgttca atcgcctgcc
    26641 ggttgctcca ggacttagtg agcgccgccg cagcaggcgc atcaagccac ttgtaagcca
    26701 gatgttcagt gaaaacgatc tgccgctcgt gcggaagcgc aagacagaac catgattccg
    26761 tattacgcgt cacgcccggc gcatagcgat gacgtaaatg tgaaaaaatt tcaaactcta
    26821 ccgtgcgctg acagtcaatt aaggtcagtt gttcagcgac aacatcaatg gtaacctctt
    26881 cctttacttc gcgcatggca gcttgcggcg cggtttcacc ctcttccacg ctgccggtta
    26941 ccgactgcca gaaatcggga tcgtcacgcc gctgcaacat cagcacccgt ttcgtatctt
    27001 gtgcatagat gaccactaag atcgaaacgg gacgcttata cactttatcc ttcacgctgc
    27061 ctcttcgttg actgccttcg ctcatcccat tcacatagtt atctatgctc atgggagttc
    27121 actcagttgc cgccttgatg caacgcgaat gatttcgtgt atttgagtca tatcagttat
    27181 tctcagcctt cttcacaacc tgaatgctca gctcagccag tgcagtcggg ttagcaaagc
    27241 tcggtgcttc agtcatcaga cacgctgccg ccgtggtttt cgggaaggcg ataacgtcac
    27301 ggatattgtc ggtgccagtc agcagcatgg tcagacggtc aagaccgaat gccagacctg
    27361 cgtgcggcgg agtaccgtat ttcagggcgt cgagcaggaa gccgaatttc tcgcgctgtt
    27421 cctcttcgtt gatacccaga ataccaaaca ccgtctgctg catatcacca ttatggatac
    27481 gtactgaacc accgcccact tcgtaaccat tgatgaccat atcgtaagcg ttcgccaccg
    27541 cattttccgg tgcagctttc agttctgcag cagtcatatc tttcggtgag gtgaacggat
    27601 ggtgcattgc cgtcaggccg ccttcaccgt cgtcttcaaa catcgggaag tcgataaccc
    27661 acagcggtgc ccatttgctt tcgtcggtca gaccaaggtc tttacccact ttcaggagca
    27721 gtgcgcccat cccgtcggca acaattttct tgttgtcggc accgaagaaa atcatatcgc
    27781 catcttgcgc ggcagtacga tccaggatgg cttcgatgat ttctgcatta aggaacttcg
    27841 ctaccgggct gttgatacct tccagacctt tcgcgcgttc gttaactttg atgtaagcca
    27901 gacctttcgc gccgtagatt ttaacgaagt taccgtattc gtcgatctgc ttacgggtca
    27961 gcgatgcgcc acccggaaca cgtagagcgg caacacggcc tttcggatcg ttcgctggcc
    28021 ccgcaaatac tgcaaactca acagatttca gcagatcggc aacgtcggtc agttccatcg
    28081 ggttacgcag atccggttta tcagaaccat aacggcgttc tgcttccgca aaggtcatta
    28141 ccgggaaatc gcccagatcc acgcccttca cttccagcca caaatgacgc accagcgctt
    28201 ccatcacttc acgcacttgc ggcgcggtca tgaaagaagt ttccacatcg atctgagtaa
    28261 attcaggctg acgatcagcg cgcaggtctt cgtcacggaa gcatttaacg atctgatagt
    28321 aacggtcaaa accggacatc atcagcagct gtttgaacaa ctgtggggat tgcggcagtg
    28381 cgtagaattt acctttgtgc acacgagaag gcaccaggta gtcacgcgcg ccttccggcg
    28441 tggctttggt cagcatcgga gtttcgatgt cgaggaagcc gtggtcatcc ataaaacggc
    28501 gcaccaggct ggtgatttta gcgcgggttt tcaggcgttg cgccatttcc gggcgacgca
    28561 ggtcgaggta gcggtatttc agacgcgctt cttcggtgtt gacgtggtta gagtcaagcg
    28621 gcagaacatc tgcgcggttg atgatagtca gcgaggacgc cagcacttca atttcgccgg
    28681 tcgccatatc gcggttaata tttttttcgt cacgcgcacg tacggtgccc gtaacctgaa
    28741 tgcagaactc attacgcagt tcagaggcca gctttaacgc gtccgcacga tccggatcga
    28801 aaaatacctg cacgatacct tcgcggtcgc gcatatcgat gaagatcagg ctaccaagat
    28861 cacgacgacg gttgacccaa ccacacagag tcacctgctg ccccacgtgg gacaaacgga
    28921 gctgtccaca atattctgta cgcatgagat atcccttaac ttagctgccg gcggatgccc
    28981 cctgctgcgc aggtgaccaa gtcgcagcgt tagctgtatg tcacaactga atgaaaaaag
    29041 gcggctatta tactggaaat tctgccgcac cgtaagagcc tggcccgcgc tggaacgcct
    29101 cgttaccact ttatatcggg cctgaaatca gactctacgc cagtttgcta taaaggtgtt
    29161 gcccgaactc ataaaaatta acaaaatttg tcgttccgcc atcggctaat cgcattaagg
    29221 tgtgaggcac gattttgttt tttcaggagt cagcatgctt gaacttaatg ctaaaaccac
    29281 tgcgttggtg gtgatcgatt tacaagaagg catcttacct tttgccggtg gtccacatac
    29341 tgccgatgag gtagttaatc gcgccgggaa gctggcggcg aaatttcgcg ccagcggtca
    29401 gcccgtgttt ctggtgcgag ttggctggtc tgccgattac gccgaagcat taaaacagcc
    29461 ggttgat
//
LOCUS       DADLTY010000033        29222 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.033, whole genome shotgun sequence.
ACCESSION   DADLTY010000033 DADLTY010000000
VERSION     DADLTY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.033"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(1..441)
                     /locus_tag="JN334_001230"
                     /pseudo
     CDS             complement(1..441)
                     /locus_tag="JN334_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(416..1216)
                     /gene="otsB"
                     /locus_tag="JN334_001231"
     CDS             complement(416..1216)
                     /gene="otsB"
                     /locus_tag="JN334_001231"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HAY7437440.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1383..2372)
                     /locus_tag="JN334_001232"
     CDS             complement(1383..2372)
                     /locus_tag="JN334_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HAY7437441.1"
                     /translation="MMSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVP
                     NFATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTES
                     LWIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIED
                     ESFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPV
                     VRTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGK
                     ISYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2384..3898)
                     /locus_tag="JN334_001233"
     CDS             complement(2384..3898)
                     /locus_tag="JN334_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HAY7437442.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3968..4957)
                     /locus_tag="JN334_001234"
                     /pseudo
     CDS             complement(3968..4957)
                     /locus_tag="JN334_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
     gene            5754..6257
                     /locus_tag="JN334_001235"
     CDS             5754..6257
                     /locus_tag="JN334_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HAY7437443.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6337..6588)
                     /locus_tag="JN334_001236"
     CDS             complement(6337..6588)
                     /locus_tag="JN334_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HAY7437444.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="JN334_001237"
     CDS             complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="JN334_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HAY7437445.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7052..7375
                     /locus_tag="JN334_001238"
     CDS             7052..7375
                     /locus_tag="JN334_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437446.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7546..8043
                     /gene="ftnA"
                     /locus_tag="JN334_001239"
     CDS             7546..8043
                     /gene="ftnA"
                     /locus_tag="JN334_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HAY7437447.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8080..8319)
                     /locus_tag="JN334_001240"
     CDS             complement(8080..8319)
                     /locus_tag="JN334_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HAY7437448.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8509..9720
                     /gene="tyrP"
                     /locus_tag="JN334_001241"
     CDS             8509..9720
                     /gene="tyrP"
                     /locus_tag="JN334_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HAY7437449.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="JN334_001242"
     CDS             complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="JN334_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HAY7437450.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10643..10729)
                     /locus_tag="JN334_001243"
     tRNA            complement(10643..10729)
                     /locus_tag="JN334_001243"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10693..10695),aa:Leu,seq:taa)
     gene            complement(10742..10815)
                     /locus_tag="JN334_001244"
     tRNA            complement(10742..10815)
                     /locus_tag="JN334_001244"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10781..10783),aa:Cys,seq:gca)
     gene            complement(10870..10945)
                     /locus_tag="JN334_001245"
     tRNA            complement(10870..10945)
                     /locus_tag="JN334_001245"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10910..10912),aa:Gly,seq:gcc)
     gene            complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="JN334_001246"
     CDS             complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="JN334_001246"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAY7437451.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="JN334_001247"
     CDS             complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="JN334_001247"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAY7437452.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="JN334_001248"
     CDS             complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="JN334_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAY7437453.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14483..14659
                     /locus_tag="JN334_001249"
     CDS             14483..14659
                     /locus_tag="JN334_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437454.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14646..14870
                     /locus_tag="JN334_001250"
     CDS             14646..14870
                     /locus_tag="JN334_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HAY7437455.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="JN334_001251"
                     /pseudo
     CDS             complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="JN334_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="JN334_001252"
     CDS             complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="JN334_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HAY7437456.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="JN334_001253"
     CDS             complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="JN334_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HAY7437457.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="JN334_001254"
     CDS             complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="JN334_001254"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HAY7437458.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="JN334_001255"
     CDS             complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="JN334_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7437459.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="JN334_001256"
     CDS             complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="JN334_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HAY7437460.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19761..20480)
                     /locus_tag="JN334_001257"
                     /pseudo
     CDS             complement(19761..20480)
                     /locus_tag="JN334_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20645..22297)
                     /locus_tag="JN334_001258"
     CDS             complement(20645..22297)
                     /locus_tag="JN334_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HAY7437461.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22563..23969
                     /gene="fliD"
                     /locus_tag="JN334_001259"
     CDS             22563..23969
                     /gene="fliD"
                     /locus_tag="JN334_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAY7437462.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23994..24404
                     /gene="fliS"
                     /locus_tag="JN334_001260"
     CDS             23994..24404
                     /gene="fliS"
                     /locus_tag="JN334_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAY7437463.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24404..24769
                     /gene="fliT"
                     /locus_tag="JN334_001261"
     CDS             24404..24769
                     /gene="fliT"
                     /locus_tag="JN334_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HAY7437464.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24847..26334
                     /gene="amyA"
                     /locus_tag="JN334_001262"
     CDS             24847..26334
                     /gene="amyA"
                     /locus_tag="JN334_001262"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY7437465.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26368..26781)
                     /locus_tag="JN334_001263"
     CDS             complement(26368..26781)
                     /locus_tag="JN334_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7437466.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26968..28173
                     /gene="yedE"
                     /locus_tag="JN334_001264"
     CDS             26968..28173
                     /gene="yedE"
                     /locus_tag="JN334_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HAY7437467.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLSWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28170..28403
                     /gene="yedF"
                     /locus_tag="JN334_001265"
     CDS             28170..28403
                     /gene="yedF"
                     /locus_tag="JN334_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAY7437468.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28510..29180
                     /locus_tag="JN334_001266"
                     /pseudo
     CDS             28510..29180
                     /locus_tag="JN334_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
BASE COUNT         7286 a         7534 c         6955 g         7447 t
ORIGIN      
        1 caatgcgatt attcacccca cgtttgcgta attcatgcgc aaagggcaac aggtgataat
       61 cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg
      121 catttacgcg tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat
      181 aatgaaaagc gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct
      241 gttcgctgag gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt
      301 ccctgtttca ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat
      361 gccaacggca aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga
      421 tactacgact aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg
      481 cggcggttat catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg
      541 cctgagttgc acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga
      601 agccagattc atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag
      661 cttcctgcat aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga
      721 caacacactt tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta
      781 atgtcattaa tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc
      841 ctttcgcctc cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa
      901 tatcacgcgc aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc
      961 gctccgcccc atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct
     1021 ccaccattga gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct
     1081 gtagtccttg cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg
     1141 ccagcgttcc atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg
     1201 ttaacggttc tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc
     1261 gctttacaga catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca
     1321 gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag
     1381 catcagacag tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg
     1441 attaagccgc gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt
     1501 tccacggtgc ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct
     1561 ttcagagaaa cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac
     1621 gtcattggct gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc
     1681 aggccagaga gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc
     1741 gcagcctctt cgttcccgcc aatcgccagg gtattacgac caaaggtggt tttattcagc
     1801 agcaaaccaa agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac
     1861 cagttagcgt aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct
     1921 gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca
     1981 tttattttca gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac
     2041 aaacccgctg ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg
     2101 acacccgcac aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag
     2161 aacaacatgc cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata
     2221 ttaatgaagg tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac
     2281 accaccagca tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg
     2341 ccagaccccg atgtagaaac agaagacatc atactctcct tactcaggca actgcctggc
     2401 tgactttagg catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt
     2461 caccggcgat ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag
     2521 gtaagtcgct ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt
     2581 tatatatttc gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga
     2641 ccttcatctc ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga
     2701 gattcatgat cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat
     2761 cggcattgtt ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc
     2821 tggcactgat gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc
     2881 gagcttccgg gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga
     2941 tcggctgttg gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt
     3001 tcattaattc gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc
     3061 gaaccgccag acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc
     3121 gctcctcgcc ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca
     3181 tcgcctgcac cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac
     3241 gtccatcttt aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag
     3301 aaacgtataa gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc
     3361 cgatttcgcg ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac
     3421 gcgccagcgc tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg
     3481 gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca
     3541 gcaatgagcg attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg
     3601 cgacggtcat ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa
     3661 gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg
     3721 ttggcgcata gttgccgctg aggattttta agagagttga ttttcctgcg ccattttcac
     3781 ccatcaacgc atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa
     3841 cgccgggaaa cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa
     3901 cgtctccgtg aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc
     3961 cggcaaatta cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg
     4021 tgatcagtac cacgtctgta acttcggtaa attttggcgt ttcaacgtct tttgctaccc
     4081 agttgtaaag catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac
     4141 cgtagaagcc ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac
     4201 caataccgat gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa
     4261 gtacggtgct gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca
     4321 gcattgagtt ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat
     4381 aaatttgttt ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc
     4441 ggcgggcggt atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc
     4501 agccacgttt ctgcatctct ttatacagtt cctagccctg acgttcgcca attttagtcg
     4561 ccgccatcat caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt
     4621 catccactgc aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga
     4681 gtttcgggtc cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga
     4741 tcgcgttcaa tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat
     4801 ccttcccggc tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct
     4861 tcaccagaaa accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca
     4921 gaccaatggc tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg
     4981 ttttgtgtag ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc
     5041 ttttatctaa aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat
     5101 aggagagcaa tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa
     5161 gacataagga aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata
     5221 agattagcct ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc
     5281 agtcaatagg ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca
     5341 aagatgtgga aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta
     5401 acagaatacg tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc
     5461 acttcatcat gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat
     5521 taaaattcat tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat
     5581 gatttaagca ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa
     5641 ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat
     5701 ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa
     5761 ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc
     5821 tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc
     5881 ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga
     5941 gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc
     6001 tggaagaact gttccaaaaa acgatggaag aatacgagca acgttcaagc actctggcgc
     6061 agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc
     6121 tggaaaaaga acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca
     6181 gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt
     6241 cacaccagct gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag
     6301 aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt
     6361 aaacttgagt tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
     6421 ggcagcggga tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac
     6481 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
     6541 taccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
     6601 atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
     6661 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
     6721 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg
     6781 cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata
     6841 atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt
     6901 aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca
     6961 agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa
     7021 tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     7081 aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc
     7141 aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg
     7201 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat
     7261 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     7321 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     7381 ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat
     7441 tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt
     7501 atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa
     7561 atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag
     7621 caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt
     7681 cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc
     7741 aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa
     7801 ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct
     7861 catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct
     7921 gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc
     7981 aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac
     8041 taatgctctc cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact
     8101 ctgatcggtc gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc
     8161 taccagctct cccgctgtca ccccttctgc cgagcacgtg tgaaaacgtg cctgttcacc
     8221 aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat
     8281 catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc
     8341 tagcgtagcg attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt
     8401 gtacatttat atttacacaa tatgtaacgt cggtttgacg aagcagccgt tatgccttaa
     8461 cctgcgccgc agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga
     8521 accctgggaa gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg
     8581 ccgctggctg cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg
     8641 gcgttgatgt gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat
     8701 accggtctgg gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc
     8761 ttcagtatga tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa
     8821 ttgttggcct ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg
     8881 ctgttgttca cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta
     8941 tttaaccgtt ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg
     9001 ctaccgcata ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg
     9061 tctgcaatcc cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc
     9121 agctatatgg atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc
     9181 cccctggtgg catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc
     9241 tttatgggat tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa
     9301 atggtggcct ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc
     9361 acgtcatttc tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt
     9421 tcaaataccg ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg
     9481 tttgcactgt tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg
     9541 gcggtactgg cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct
     9601 caggcgggtt accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt
     9661 attgctgtga ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga
     9721 tcagatagcc tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct
     9781 cttaatgcag gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac
     9841 gccccggttt ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca
     9901 tccagtatgc atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg
     9961 cttctggcga cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg
    10021 cctccagcgc tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc
    10081 gcatatagcc aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca
    10141 agccgaataa cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca
    10201 tatgttgaaa agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca
    10261 catagtcagc cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag
    10321 aactcaacac cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag
    10381 tgttgtattt ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg
    10441 ttttcatacg cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc
    10501 gtatcagaga tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg
    10561 acaagttgca ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt
    10621 gattttattc ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa
    10681 cgccgaggga ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa
    10741 gtggaggcgc gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg
    10801 ctttgttaac gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt
    10861 cttttaaact ggagcgggaa acgagactcg aactcgagac ctcgaccttg gcaaggtcgt
    10921 gctctaccaa ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat
    10981 tatcgtctgg caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg
    11041 agtcaacgat atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac
    11101 tgatcaagca aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt
    11161 accgcagcca caaagaaaag tgcaataccg acgtactcaa cccaaatgtt cggacgccac
    11221 agcagccagg ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg
    11281 gccacgctac tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt
    11341 tcacgggcga tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt
    11401 accagcacca tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca
    11461 aaccgggtac tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc
    11521 gcgacgcaga aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc
    11581 aatacaaaga atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat
    11641 tgcataatga cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac
    11701 atcaatgttt caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt
    11761 ttgcaatttc ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca
    11821 acatttgccg acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga
    11881 ccttcgcccg ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat
    11941 gctggataac atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct
    12001 caaagaacag cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca
    12061 gcggatgatt tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa
    12121 gctggccttt gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt
    12181 taccataacg ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc
    12241 ctgtaatgtt atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac
    12301 aggaagcgac ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca
    12361 cttccggcaa tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct
    12421 gcgaaagttt gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg
    12481 ccctatcgcc gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg
    12541 aatcggcgag cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg
    12601 tgcgcatctg gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca
    12661 gttccgtacc gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac
    12721 gaatgaacaa tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa
    12781 ggtcgtcgcc ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt
    12841 ggtcgcgaat acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc
    12901 taatgagttg cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga
    12961 cctgctgagc gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc
    13021 ctatctggta ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc
    13081 gaatggggaa aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga
    13141 acgggccgaa atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt
    13201 gggtatcgcc actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt
    13261 aacgcggctg atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat
    13321 gcgtaaccgt tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag
    13381 cgaggttgct gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat
    13441 agataaccgt accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt
    13501 ttaaaaacgc ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca
    13561 cagaccatgg cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact
    13621 gaacatacga tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat
    13681 ctcattgacc ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa
    13741 actggcaaat gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc
    13801 gatatcagaa gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc
    13861 ctgcggagcc gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg
    13921 taaagggttt tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc
    13981 gattttacgc gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac
    14041 gtcaacggca tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac
    14101 gacttttata cccttaatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg
    14161 gtcatcaaca agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact
    14221 gatagttagt cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt
    14281 gggtattcga tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc
    14341 gtcaccaacg gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat
    14401 tgactttatt cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga
    14461 acaatacggg caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt
    14521 ttatagcact gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat
    14581 ggtatactcc gccgccttaa catttttcac cacaaatttt cattgcaaca tgacgaggaa
    14641 aataaatgag tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag
    14701 tctcctgcct gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg
    14761 gccgcgtgat gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg
    14821 cagtattttc caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg
    14881 gctgagagcg tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct
    14941 tcccttccgc cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg
    15001 tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact
    15061 gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa
    15121 aggacaaaaa gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta
    15181 aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc
    15241 acattaaatg gccctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat
    15301 tttttgcctg ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag
    15361 gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct
    15421 gagcctgaag ctctatttca tggtaggcct cttctgcggc ctccatcctc tgaaaacgca
    15481 acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc
    15541 ctgagagtga gtatcattta taatgataat tcaaactatc acataaataa gatttatata
    15601 taatttatat tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat
    15661 taatgagtta tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg
    15721 acaggtgaat cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac
    15781 tggcgggtgc gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact
    15841 atccgcccct ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg
    15901 tgtgtcacaa tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact
    15961 tcgcccacca gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga
    16021 cgcattgcca gtgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt
    16081 ggatagctgg tttctttacc ggccagccca actttcgcca gcagctcgcg agcgcgcgcc
    16141 gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc
    16201 accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac
    16261 tggcgaatca gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca
    16321 acggtgatcg ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt
    16381 ttgccggaac cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg
    16441 ataccgtgca gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca
    16501 ctcattttgg ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg
    16561 ataacacagt cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct
    16621 ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg
    16681 cggccagcga cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca
    16741 cccgcgccgc ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac
    16801 tggcagcggc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg
    16861 cggcataggc ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt
    16921 cgataccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac
    16981 cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc
    17041 gcatcagcgc gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt
    17101 gcagggtata cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac
    17161 tttcttgcat gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc
    17221 gccgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc
    17281 gtcaatcagc cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag
    17341 acgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc
    17401 aaaatagtca tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat
    17461 cgcctgttgt aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac
    17521 gccaatcagt tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt
    17581 tccggcactg cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg
    17641 cgcgatttcc agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac
    17701 cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagtt cttccagttg
    17761 ggcattggga tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa
    17821 caaacgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag
    17881 cgccacgcag tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga
    17941 ctggatcgcg ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag
    18001 aaattccagc ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt
    18061 gatgaaaatt tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg
    18121 cgcgccgata aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc
    18181 cgatgtgtgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc
    18241 gctagtgaaa gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac
    18301 catttttcgg aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc
    18361 accgctttca gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag
    18421 aatgcttcac cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc
    18481 agacgatcaa cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg
    18541 tcatcatcat aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag
    18601 ttggtgccca gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg
    18661 ccttcgttac cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa
    18721 tcgtattttt tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt
    18781 ttagagtcca gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg
    18841 ccaagatgtt ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca
    18901 tccccctgaa aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgctc
    18961 tctttgactt tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc
    19021 agcgccacgg ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc
    19081 accccgaatg ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca
    19141 actccgctac atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc
    19201 tgcacaagtc cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg
    19261 taattgttgg tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc
    19321 tgcagctgat cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga
    19381 acgacatatt cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta
    19441 aagccgataa tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg
    19501 cacaatgccg cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc
    19561 aggcgggaaa tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg
    19621 agtaatttac ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg
    19681 cttaatggcc gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta
    19741 aagtgcggca tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatggcc
    19801 tggctgtgta actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg
    19861 agattcagct cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt
    19921 tcgatggctt ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt
    19981 tctcgctgat gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca
    20041 tcgtaggaga agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata
    20101 tcgatcccta aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc
    20161 agttgcccta ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag
    20221 tcacggctgc gaagttcatc cagcatagcg ccacggatac gctacactgc gtaagttgta
    20281 aatgccgttc cttgtagggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc
    20341 gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct
    20401 tcgtgacgca ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca
    20461 gcggtataga gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta
    20521 tccgtttctg cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg
    20581 gttacgcaaa aagccgaatg taaaaaaccc cgccggtggc ggggtttgag aggcattcag
    20641 acgattaacc ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca
    20701 cggagttacc ggcctgctgg ataatctgcg ctttcgacat gttggacact tcggtcgcat
    20761 agtcggcgtc ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt
    20821 tggtgactgc ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga
    20881 tggagctgat ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt
    20941 cagcatctgt cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag
    21001 tcactgaacc atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt
    21061 tgttatcaac ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc
    21121 tataacctgc agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag
    21181 ctgcctgatc aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat
    21241 taccggtaaa ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg
    21301 ctgcaagtgt ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc
    21361 gcccagttgt atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc
    21421 tgatctgaac atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa
    21481 ctcccggggt caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt
    21541 atgacttact tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt
    21601 tattaatggt tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt
    21661 tagcagcatc gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag
    21721 ctaccaagtc agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa
    21781 acccattcag ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc
    21841 cgtcattcgc accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt
    21901 tgaactgggt ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct
    21961 gaatggagtc cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac
    22021 ggatacgctg taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag
    22081 aaataccgtc gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac
    22141 ggttagcaat cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag
    22201 acagacgctc gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga
    22261 tcagcgagag gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag
    22321 tcgttgatta cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt
    22381 ctgtcgtaaa acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga
    22441 aatactcttt tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt
    22501 gcgcaattca ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc
    22561 gcatggcaag tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg
    22621 atagcctcac cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt
    22681 ttaccgctaa acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg
    22741 ccaatactgc attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca
    22801 ccgcgttcag tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca
    22861 cccatctggc gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg
    22921 cgatcgccac cagcgacagc aaactcacca ttcaacaagg cgacgacaaa gatccgatta
    22981 ccattgatat cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg
    23041 caaaagcggg cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag
    23101 tcacatcaaa tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg
    23161 cgctacaaag ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg
    23221 tcgccgccca gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca
    23281 ctatcagcga cgcgctggaa aacatcactc tgaacctgaa cgatgtcacc acgggcaacc
    23341 agacgctaac catcactcag gacacctcca aagtgcaaac ggcgattaaa gactgggtga
    23401 atgcctataa ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg
    23461 cgggagctga tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga
    23521 cgattcagac gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa
    23581 cgctggcgca gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg
    23641 acaaactcac cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg
    23701 atggtaaaaa aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga
    23761 caacgggcat tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta
    23821 aagactacaa cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat
    23881 ttacccaact ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc
    23941 agttcgaaaa caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg
    24001 cggcaaaagg cacccaggcc tatgcacaaa ttggcgtcga aagcaccgta atgagcgcca
    24061 gccaacagca gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc
    24121 gactgtttat gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca
    24181 acatcattga gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc
    24241 aaaacttaat tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca
    24301 acgatgtctc agcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga
    24361 aagagtcttt actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca
    24421 tttgtatttc gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac
    24481 ggaagaacaa tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga
    24541 gatcgcacac ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc
    24601 gatgctgcac ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat
    24661 ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta
    24721 tggcgatcag ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag
    24781 ccgatggctc gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt
    24841 cgaagaatgc gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt
    24901 aagctctggc ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg
    24961 gtctggttgc cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc
    25021 tatgatttat ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt
    25081 gataaagcac aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg
    25141 ctggacgtgg tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag
    25201 cgtgtaaatg ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg
    25261 acgcgttaca ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa
    25321 tgttttagcg gtatcgacca tatcgaacac cctgatgaag atggcatttt taaaattgtt
    25381 aacgactata ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat
    25441 tatctgatgg gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac
    25501 tgggcgcgct gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa
    25561 catattccgg catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag
    25621 ccgctgttta ttgtggctga atgctggtcg catgaagtcg ataaactgca aacgtatatt
    25681 gatcaggtgg aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa
    25741 gcatcgcgca tgggtcgcga ctacgacatg acgcagatct tcactgggac gctggtggaa
    25801 gccgatcctt ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc
    25861 ctcgaagcac cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat tttgttgcgg
    25921 gaaaatggcg ttccttcggt gttctatccg gacctctacg gtgcacatta cgaagatgtc
    25981 ggtggtgacg ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta
    26041 attctcgccc gtcagcgttt cgcccacggt gtacagacat tatttttcga ccatccgaac
    26101 tgcattgcct ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg
    26161 aacggagatg atggcgaaaa aaccattcat ctgggagaga attacggcaa taaaacctgg
    26221 cgtgattttc tggggaaccg gcaagagagc gtagtgacag acgaaaacgg cgaagcgacc
    26281 ttcttttgca acggcggcag cgtcagcgtg tgggttatcg aagaggtgat ttaaatccat
    26341 ccccggcggc aagccgggga gatttcatta cggcagtggc gtcggtaacg gcgctttatc
    26401 cagcgcggcg gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta
    26461 ctcaatggtg ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt
    26521 gagatcatca gacatcaatg tcaacttacc cggcacggca atcccccgct gccactgacg
    26581 gcaatccaag gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccggactgac
    26641 cagcgcacgt tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt
    26701 tttcacgaca ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc
    26761 tgcaattgcg agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt
    26821 aaagctctga tgtcgccagc ctttccaggt tcagagagaa atttgatcta actattattt
    26881 ttgtgaatta ttaccccgaa aataaatttg catggtgtat gattcgcggc tcttgtttca
    26941 aaccttctga gttcagaggc tacattcatg tcatggcagc aattcaaaca cgcctggttg
    27001 attaaattct gggcacccat ccctgcggtc atcgcggcgg gtattctctc cacttactat
    27061 tttggcatta ctggcacctt ttgggctgtc acgggtgaat ttacccgttg gggcggtcag
    27121 ctcctgcagc tgtttggcgt ccatgctgaa gagtggggtt attttaaaat tatccatctg
    27181 gaaggatcgc cattaacccg gatcgacggc atgatgatcc tcggtatgtt tggcggctgc
    27241 tttgctgctg cgctgtgggc caacaatgtc aaactacgca tgccacgcag ccgtatccgc
    27301 attatgcagg ccatcattgg cggcattatc gccggttttg gcgcgcgtct ggcaatgggc
    27361 tgtaacctgg cggcgttctt taccggcatt cctcagttct cgctgcatgc ctggttcttt
    27421 gccatcgcca ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct gcccatcttc
    27481 cgtattcccg tgaaaatgca gaaagtttct gccgcctcac cgctgacgca aaaaccggat
    27541 caggcgcggc gtcgttttcg tctcgggatg ctggtctttt tcggcatgct gagctgggcg
    27601 ctgctcacgg cgatgaacca accaaaactg gggctggcaa tgctgtttgg cgtcggcttt
    27661 ggtttactga ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga tatgtggatc
    27721 accggacgta cccatatggc gaaagcaatc attatcggta tggcggtgag tgccatcggg
    27781 atcttcagtt acgtacagtt aggcgttgaa cctaaaatca tgtgggcggg accaaatgcg
    27841 gtaattggtg gtttactttt tggttttggc atcgtgctgg caggcggctg cgaaaccggc
    27901 tggatgtacc gcgcggtaga aggccaggtg cactactggt gggtcggtct gggcaacgtg
    27961 atcggctcaa cgattctggc gtattactgg gatgatttcg ctccggcgct ggccaccgac
    28021 tgggacaaaa tcaacctgct gaaaactttt ggtccgatgg gaggcctgct ggtgacatat
    28081 ttgctgctgt ttgctgcgct gatgttgatt atcggctggg aaaaacgctt cttccgccgt
    28141 gaggcaccgc agactgctaa ggagatcgca tgaaaaatat cgttcctgat taccgccttg
    28201 atatggtggg tgaaccctgc ccttatccgg cagtcgcaac ccttgaggcg atgccgcaat
    28261 taaaaaaagg tgagatcctg gaagtggtga gcgactgtcc gcagtcgatc aataatattc
    28321 cactggatgc gcgtaatcac ggctacacgg tgctggatat tcagcaagac gggccgacca
    28381 ttcgttattt aattcagaag taatccctca ttcccgctgt ggcccacagc gggaacatcg
    28441 ttacctctga cacatagatt ttacccgccc cctctcctat tcttttcagt atgatctcag
    28501 gaggttgcta tgtgtggacg ctttgcccaa tcccaaacgc gtgaggatta ccttgcgctt
    28561 ctcgcggaag atattgaacg cgatattccc tacgatcccg aacccattgg cagatacaat
    28621 gtcgcgccgg gaaccaaagt tcttctgctc agtgaacgtg atgaacacct tcatctggat
    28681 ccggttttct ggggatatgc tcccggatgg tgggataaac cgccgctgat taacgcccgc
    28741 gtagaaactg cctcaaccag tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc
    28801 tgttttgccg atggctggtt tgagtggaaa aaagaaggcg acaaaaaaag ccttatttta
    28861 tctatcgcgc tgacggacaa cctgttttta tggccgcgat aggtagcata ccgtttgagc
    28921 gtggtgacga agccgaagga tttttgatcg tcactgcggc ggcagatcaa ggtctggtag
    28981 atattcatga ccgccgcccg ctggttctgt cgccagaagc tgcacgggaa tggatgcggc
    29041 aagacattgg tggtaaagaa gcctcagaaa tcgctaccag aagctgtgtg ccagcaaacc
    29101 agttcacctg gcaccctgtg tcgcgcacgg tgggtaatgt caaaaaccag ggcgcggagt
    29161 taattcaacc tgtttgttga catcctccac gccctgaatg acgaggaccc ctgctacgtt
    29221 ca
//
LOCUS       DADLTY010000034        28741 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.034, whole genome shotgun sequence.
ACCESSION   DADLTY010000034 DADLTY010000000
VERSION     DADLTY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.034"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..205
                     /locus_tag="JN334_001267"
     CDS             <1..205
                     /locus_tag="JN334_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="ethanolamine utilization protein; involved in the
                     degredation of ethanolamine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ethanolamine utilization protein EutK"
                     /protein_id="HAY7437469.1"
                     /translation="EPDAPVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARN
                     ALEQLFSAGTLRKRSSRYRLKPH"
     gene            251..1303
                     /gene="eutR"
                     /locus_tag="JN334_001268"
                     /pseudo
     CDS             251..1303
                     /gene="eutR"
                     /locus_tag="JN334_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(1309..2208)
                     /gene="hemF"
                     /locus_tag="JN334_001269"
     CDS             complement(1309..2208)
                     /gene="hemF"
                     /locus_tag="JN334_001269"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAY7437470.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(2212..3081)
                     /gene="amiA"
                     /locus_tag="JN334_001270"
     CDS             complement(2212..3081)
                     /gene="amiA"
                     /locus_tag="JN334_001270"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HAY7437471.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            3295..3720
                     /locus_tag="JN334_001271"
     CDS             3295..3720
                     /locus_tag="JN334_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase YpeA"
                     /protein_id="HAY7437472.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            3707..4156
                     /locus_tag="JN334_001272"
     CDS             3707..4156
                     /locus_tag="JN334_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HAY7437473.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            4217..4792
                     /locus_tag="JN334_001273"
     CDS             4217..4792
                     /locus_tag="JN334_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HAY7437474.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            4888..5787
                     /gene="yfeX"
                     /locus_tag="JN334_001274"
     CDS             4888..5787
                     /gene="yfeX"
                     /locus_tag="JN334_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HAY7437475.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(5967..7391)
                     /gene="murP"
                     /locus_tag="JN334_001275"
     CDS             complement(5967..7391)
                     /gene="murP"
                     /locus_tag="JN334_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HAY7437476.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(7395..8291)
                     /gene="murQ"
                     /locus_tag="JN334_001276"
     CDS             complement(7395..8291)
                     /gene="murQ"
                     /locus_tag="JN334_001276"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAY7437477.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            8254..9255
                     /gene="murR"
                     /locus_tag="JN334_001277"
     CDS             8254..9255
                     /gene="murR"
                     /locus_tag="JN334_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HAY7437478.1"
                     /translation="MFEPSVIIFSSCIGGDLTIYDQIIADVWNHISRIDRLKGIIEST
                     LAECEERHKNLSGIFAASPGEWKMLYLTKIRNAESEFTGNEQKIADFLRARVSELKSV
                     SSRQMAKQLGISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSIT
                     SDDSLEVIARKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGR
                     DLSFKLMKIGYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQG
                     ATVIAITSLADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            9442..10233
                     /gene="ucpA"
                     /locus_tag="JN334_001278"
     CDS             9442..10233
                     /gene="ucpA"
                     /locus_tag="JN334_001278"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HAY7437479.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            10537..11553
                     /locus_tag="JN334_001279"
     CDS             10537..11553
                     /locus_tag="JN334_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7437480.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            11553..12386
                     /gene="cysT"
                     /locus_tag="JN334_001280"
     CDS             11553..12386
                     /gene="cysT"
                     /locus_tag="JN334_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HAY7437481.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            12386..13261
                     /gene="cysW"
                     /locus_tag="JN334_001281"
     CDS             12386..13261
                     /gene="cysW"
                     /locus_tag="JN334_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HAY7437482.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            13251..14348
                     /gene="cysA"
                     /locus_tag="JN334_001282"
     CDS             13251..14348
                     /gene="cysA"
                     /locus_tag="JN334_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HAY7437483.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            14482..15393
                     /gene="cysM"
                     /locus_tag="JN334_001283"
     CDS             14482..15393
                     /gene="cysM"
                     /locus_tag="JN334_001283"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HAY7437484.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(15597..16320)
                     /locus_tag="JN334_001284"
                     /pseudo
     CDS             complement(15597..16320)
                     /locus_tag="JN334_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(16353..16727)
                     /locus_tag="JN334_001285"
     CDS             complement(16353..16727)
                     /locus_tag="JN334_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HAY7437485.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            16832..17683
                     /gene="pdxK"
                     /locus_tag="JN334_001286"
                     /gene_synonym="thiJ"
     CDS             16832..17683
                     /gene="pdxK"
                     /locus_tag="JN334_001286"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HAY7437486.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(17725..18234)
                     /gene="crr"
                     /locus_tag="JN334_001287"
     CDS             complement(17725..18234)
                     /gene="crr"
                     /locus_tag="JN334_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAY7437487.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(18275..20002)
                     /gene="ptsI"
                     /locus_tag="JN334_001288"
     CDS             complement(18275..20002)
                     /gene="ptsI"
                     /locus_tag="JN334_001288"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HAY7437488.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(20047..20304)
                     /gene="ptsH"
                     /locus_tag="JN334_001289"
     CDS             complement(20047..20304)
                     /gene="ptsH"
                     /locus_tag="JN334_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HAY7437489.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(20688..21659)
                     /gene="cysK"
                     /locus_tag="JN334_001290"
     CDS             complement(20688..21659)
                     /gene="cysK"
                     /locus_tag="JN334_001290"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAY7437490.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(21844..22605)
                     /gene="cysZ"
                     /locus_tag="JN334_001291"
     CDS             complement(21844..22605)
                     /gene="cysZ"
                     /locus_tag="JN334_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HAY7437491.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            22835..23821
                     /gene="zipA"
                     /locus_tag="JN334_001292"
     CDS             22835..23821
                     /gene="zipA"
                     /locus_tag="JN334_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAY7437492.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            23892..25907
                     /gene="ligA"
                     /locus_tag="JN334_001293"
     CDS             23892..25907
                     /gene="ligA"
                     /locus_tag="JN334_001293"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAY7437493.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            25909..26127
                     /locus_tag="JN334_001294"
     CDS             25909..26127
                     /locus_tag="JN334_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437494.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(26124..27122)
                     /locus_tag="JN334_001295"
     CDS             complement(26124..27122)
                     /locus_tag="JN334_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HAY7437495.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            27212..28138
                     /locus_tag="JN334_001296"
     CDS             27212..28138
                     /locus_tag="JN334_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7437496.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(28129..28470)
                     /locus_tag="JN334_001297"
     CDS             complement(28129..28470)
                     /locus_tag="JN334_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flxA-like family protein"
                     /protein_id="HAY7437497.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(28590..28715)
                     /locus_tag="JN334_001298"
     ncRNA           complement(28590..28715)
                     /ncRNA_class="other"
                     /locus_tag="JN334_001298"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         6919 a         7139 c         7744 g         6939 t
ORIGIN      
        1 ggaacccgac gcgccagtta tcgtcgcgga atctgctgac gagttgttgg cgctgttaac
       61 atcagtatgt cagggaatga cggcaggaga agtggccgcc cactttggct ggccgctgga
      121 aaaagccaga aatgcgctcg aacagctctt ttctgccggg acgttacgta aacgcagtag
      181 tcgttatcgt ctcaagcccc attaacctgt cggaggtgcc gggtgtccta caacacccgg
      241 cattaacatc atgaaaaaga cccgtacagc caatttgcac catctttatc atgaaccctt
      301 acccgaaaac ctgaagctca cgccgaaggt cgaagtggat aatgttcatc aacgacagac
      361 aacggatgtc tatgaacatg ctttgacgat taccgcctag cagcagattt acgatcagct
      421 gcatccgggc aagtttcatg gtgaatttac ggaaattcta ctcgatgata ttcaggtttt
      481 tcgtgaatac accggtctgg cgctgcgtca gtcgtgcctg gtctggccga actcgttctg
      541 gtttggcatt ccggcgacgc gcggtgagca gggatttatc ggttcgcaat gtctgggaag
      601 cgcagaaata gcgacgcgcc ccggtggaac cgagtttgaa ttaagtacgc cggacgatta
      661 tacgatcctg ggcgtagtgc tgtctgaaga tgtcatcacc tgtcaggcta actttttgca
      721 caatccggat cgggtattac atatgctgcg tagccagtcg gcgctggaag tgaaagagca
      781 gcataaagcc gcgctgtggg gctttgtcca acaggcgctg gcgacgtttt gcgagaaccc
      841 ggaaaatctc catcagcctg cagtgcgaaa agtgctgggg gataatttgc taatggcgat
      901 gggggcgatg ctggaagaag cgcaaccaat ggtgacggcg gaaagcatca gtcatcagag
      961 ttaccgtcgg ttgctttccc gcgcccgtga atatgtgctg gaaaacatgt ccgaaccggt
     1021 gacggtgctg gatttgtgta atcaactgca tgtcagtcgc cgcacgctac aaaacgcgtt
     1081 tcacgctatt ttaggcattg gcccaaacgc gtggctgaaa cgcattcgcc tgaacgtcgt
     1141 acgccgcgaa ctgataagcc cgtggtcgca aagcacaacg gtaaaagacg ccgccatgca
     1201 gtggggattc tggcatctgg ggcaatttgc cacggattac cagcagctgt ttgccgagaa
     1261 gccgtcgttg acgttgcatc agcggatgcg ggaatggggg tgagggagtt acacccaatc
     1321 cctgacctta ataaactcac ttaacgccgc ttctgggctg ccatcttttg gctgataatc
     1381 atactcccag cgtaccagtg gcggcattga catcaggatc gactcggtgc gcccgccagt
     1441 ttgcaggcca aacagcgtgc cgcgatccca gaccagattg aactcgacat aacgaccgcg
     1501 acggtagagc tgaaaattgc gctcgcgctc gccgtaggcc atcgctttac gtcgctctac
     1561 aattggtaaa taagcgtcgg tgtagccttt gcctaccgcc tgcataaagg caaaacagtg
     1621 gtcgaaatct ggcgtgttca gatcatcaaa gaacagcccg ccaatgccgc gctgttcgtt
     1681 gcgatgtttg aggtagaagt aatcgtcgca ccactttttg taacggggat aaacgtcttc
     1741 accaaatggc tggcacaggt cacgggcggt gcggtgccag tgaatggcgt cttcttcaaa
     1801 accatagaaa ggggttaaat cgaagccgcc gccaaaccac caaacgggct ctgcacccgg
     1861 tttttcggca ataaaaaaac gcacattcgc gtggctggtg ggaacatacg ggttatgcgg
     1921 atgcactacc agtgaaacgc ccatcgcctc gaaactgcgc ccggcaagtt ccgggcgatg
     1981 agcggtggcg gaagcaggca tcgcctcacc gtggacgtga gaaaagttga cgcccgcctg
     2041 ttcgaaaaca ccaccattac gcaacacccg actgcgcccg ccgccgccag cttcgcgctg
     2101 ccaactatct tcgacaaatt ctgcgccatc gacggcggtc agctgctgac aaatcgtatc
     2161 ctgaaggttg agcagaaact gtttaacctg gtgtgcgtcg ggtttcataa cttatcgctt
     2221 tttcgaatgt gctttctggt tgtcgaacca gtggaaataa ctgatcacgc cttcagcaat
     2281 cgctgtggcg attttctgac gaaacgccgc cgtgcctaac agccgctctt cttccgggtt
     2341 ggtgataaac gaggtttcca ccagcaccga aggaaccgac ggcgatttca acaccacaaa
     2401 cgcggcttgt tcggtgttgc ggctgtgcag tttatgcacc ggcttaatct ttttcagaat
     2461 atgcgagcca agcgtcaggc tgtttttaat agtatcggtt tgcaccagat caaacagtac
     2521 ttgctgcaat aggtgatcct tgtcagtcgc ctttttaccg gcaacttcat cggcgcggtt
     2581 ttcgcgttca gacaggtatt tcgccattgc gctacttgcc ccacggttag agagggcaaa
     2641 taccgaagca ccggcagctt tcgggttggt aaagccatcg gcatgaattg acataaacag
     2701 atctgcgcca tgtttatggg cgatttcaac gcgatcgtaa agtgggataa acgtatcgcc
     2761 agaacgcgtt aaacgcgcat caatcccatg attacgcaaa atggaacgga cgtttttagc
     2821 aatcgccagc accacatgtt tttctttcga accgttgcgt ccgatcgctc cggtatcaat
     2881 tccgccgtgg cctggatcga gaacaacgac acgtttgccg ccagattttt tggctttcgg
     2941 cttgctgtgt ccgttgctgg tttttaaagg ttcgtctttg gcaatggctt gcgacattcc
     3001 tgacaacgtc agggcagcta aaccggcttt cagcacctgg cggcgcgaag tgagtgtttt
     3061 tagtggttta aaagtgctca tacggcctga gttgtaataa taaagttcca gatgttatat
     3121 cgtatcgcgt aatccgttac gacggtttgc tgttgagaat tgttttactt ttcatttcaa
     3181 tagttgacaa ctccccatta tgccactttt tcaaccgatt ttcgtcagat attggctaat
     3241 tcgaccgttc acgccataat cagcctttta acccgcaaaa aggtaacgaa taccatggag
     3301 atacgcgtat ttcgccagga agatttcgaa gaggtcatca ccctttggga gcgttgcgac
     3361 ttgctgcgtc cgtggaacga tccggaaatg gacatcgagc gtaagatgaa ccatgacgtc
     3421 agtttgtttt tggtcgctga ggtaaacggt gaagtggtcg gaacggtgat gggcggttat
     3481 gacgggcatc gcgggtctgc ttattatctt ggcgtgcatc cagagtttcg tgggcgtgga
     3541 attgccaatg cgttgcttaa ccggctggag aaaaagctga ttgctcgcgg ctgcccgaaa
     3601 attcagatca acgtgccgga agataacgac atggtgctcg gaatgtatga acgcctggga
     3661 tatgaacacg ccgacgtgct gagtctgggt aagcgtttga ttgaagatga agagtactga
     3721 gttccatcct gtccattatg atgcgcacgg acgcctgcgt ttacccttgc tcttctggct
     3781 tgtgctattg cttcaggcgc gaacctgggt gctgtttgtc attgccggtg cgtcgcgtga
     3841 gcagggtact gcgctgctaa atctgtttta tcccgatcac gataatttct ggctggggct
     3901 gattcccggt attccggcgg tgctggcgtt tttgttgagc ggtcggcggg cttcgtttcc
     3961 tcgcacctgg catgtgctct attttctgtt gctgttggcg caggtggttt tactttgctg
     4021 gcaaccgtgg ctgtggctga acggtgaatc cgttagcggt atcggtctgg cgctggtagt
     4081 ggcggatatt gtggcgttaa tctggctgct gaccaatcga cgtttacgcg cttgttttaa
     4141 tgaagaaaaa gaataacggc actttttggt gaatttgcac tccaagcaac gttattgaat
     4201 aaccaaagga agtgacatga aatcgctgcg tttaatgtta tgcgctatgc cgttgatgct
     4261 gaccggctgt tccacgatgt cgtcagttaa ctggtctgcc gctaacccgt ggaactggtt
     4321 tggatcgtcc accaaagtga gcgagcaggg cgtgggtgaa ttaacggcgt ccacaccact
     4381 gcaagaacaa gccattgctg atgcgcttga tggcgattat cgcctgcgca gcggaatgaa
     4441 aaccacgaac ggcaacgtgg tgcgcttttt tgaagtgatg aaaggcgaca acgtggcgat
     4501 ggtgattaac ggcgatcaag gcacgatcag tcgcattgac gtgctggata gcgatattcc
     4561 tgctgacacc ggtgttaaaa tcggtacacc gtttagcgac ctttacagca aagcatttgg
     4621 caattgccaa aaagctgatg gtgatgataa tcgtgccgtc gaatgtaaag ccgaaggcag
     4681 tcaacatatt agctaccagt tcagcgggga atggcgtggt cctgaagggt taatgccttc
     4741 ggacgatacc ctgaaaaact ggaaagtcag taaaattatc tggcggcgtt aatttgcgtc
     4801 tgaacaaacc gccgtcgcga aaaaacacgt aaaatagcgc tcaacaatgc cacggattgc
     4861 gtggcattct tattttcagg aggaacaatg tctcaggttc agagtggcat tttgccagaa
     4921 cattgccgcg cggcgatttg gatcgaagcc aacgtgaaag gggaagttga cgccctgcgt
     4981 gcggccagta aaacatttgc cgacaaactg gcaacttttg aagcgaaatt cccggacgcg
     5041 catcttggtg cggtggttgc ctttggtaac aacacctggc gcgctctgag cggcggcgtt
     5101 ggggcagaag agctgaaaga ttttccgggc tacggtaaag gccttgcgcc gacgacccag
     5161 ttcgatgtgt tgatccacat tctttctctg cgtcacgacg taaacttctc tgtcgctcag
     5221 gcggcgatgg aagcctttgg tgactgcatt gaagtgaaag aagagatcca cggcttccgt
     5281 tgggtggaag agcgtgacct gagcggcttt gttgacggta cagaaaaccc ggcgggtgaa
     5341 gagacgcgcc gcgaagtggc ggttatcaaa gacggcgtgg attccggcgg cagctatgtg
     5401 tttgtgcagc gttgggagca caacctgaag cagctcaacc ggatgagcgt tcacgatcag
     5461 gagatgatga tcgggcgcac caaagaggcc aacgaagaga ttgacggcga cgaacgtccg
     5521 gaaacctctc acctcacccg cgttgatctg aaagaagatg gcaaagggct gaagattgtt
     5581 cgccagagcc tgccgtacgg caccgccagt ggcactcacg gtctgtactt ctgcgcctac
     5641 tgcgcgcgtc tgcataacat tgagcagcaa ctgctgagca tgtttggcga taccgatggc
     5701 aaacgtgatg cgatgttgcg tttcaccaaa ccggtaaccg gcggctatta tttcgcaccg
     5761 tcgctggaca agttgatggc gctgtaagcg tttctgattc attgattaaa ggccaggcag
     5821 taacattact gactggcctt tttattactg ctttaacgcc gcatatactt gatcaactac
     5881 cttctgtcag aacagggtat tttgcggcgc tgacgctaca actaacagcc agcagagaaa
     5941 gatagagcat tgtccgtttc ataatatcag tccagattga cgttacggca gccaaagagc
     6001 gtggtgaaaa taaacccgca aacccatgcc accagaatcc cgccagcata aaccgccatt
     6061 gccggaagga tgccttgtgc ggaagtcatc agcggcagcg ccaccagacc agacggccca
     6121 aaggcgctgt ttaagcccat cggcagaccc caccaggcta tcaatccgat aaacaaaccg
     6181 cccgccgcgc cgcctaaaca ggcggtaaca aacggcttca tgcggggcag ggtgacaccg
     6241 taaatcagcg gttcaccaac gcccagcagg ccgggaataa tcgccccgcg tacctgactg
     6301 cgtagcgcac tgtgcggttg cgcccgccag tagagtgcca gcgccgcgcc cacctggccc
     6361 gcgcctgcca ttgaaaggat gggaaataag ctgttgaatc cctggctgtc cattaacgcg
     6421 aggtaaacag gaataaagcc ctgatgcacg ccaaatacca cggcgatcag gaacagcccg
     6481 gctaaaaccg cacaaccgaa cggattactg ttcaggtgca taaacagcca tgacatacct
     6541 tcgaataacc agccacccag cggcataatg atcaggtacg caagcgtagc ggtgatcagc
     6601 agggtgatta acgaggtcag cagcatgtcg agatcgtccg gcataaagcg gcgcaccatg
     6661 ccttcaatgc gggcgcaggc ccacgcggca atcagcacgc cgataatatt gccgcgcgga
     6721 tcgatgggca gaccaaagaa atcgtgaaaa ccggcgtagt aaccggtggt tgcagcgggg
     6781 ttataaccga gcaaaaagag tgcggcaata attgcgccat tgacgcccgt gccgccaaat
     6841 gcctgggcgg cgttatagcc caccagaatc actaggaagg tgaacaaacc tttgctgaac
     6901 accttcataa aattcagcgc gtcgggtagc gtgccctgag catctgccgg aacgtgcatc
     6961 accgtggcaa ttaacgtcgc tatccccagc agcagaccgg cggcaataaa accggggatc
     7021 agcggcgtga agatggtggc gaatttggcg agaaattgtt gcacgccgga ggtttgtttg
     7081 gcttttaact gacgtttgtt ctgggcggcg atttctgcgg catcctgtac cggtgcatcg
     7141 cccagcagct cgctcatcgc ttttgcggca cgatgcgctt taccaggccc aaaaacgacc
     7201 tgcacctggt cgctggtcaa aatgacgccc ttcacgcctt ccagcgtttt gatattggga
     7261 tcaaccagtg aactgtcatg tacacccaga cgcaggcgcg tcatacagtt accacaactg
     7321 gcgatatttc ccggtccgcc gacacgggta agaatggtgt tcagaagttc actgctgatc
     7381 tctttggcca tgggttattc cttgtctaaa acctgacgaa taaagccgcc gtgttgatcc
     7441 aggcgttttt tagcttctgc ggcatcgaga tttttcagca ccatcacaat ggccgtttta
     7501 cagttgcgct cgcaggcaat taacgccgct tccgcttgtt cggcattaca tccggtggcg
     7561 tttttaacga tattgacctg gcgcacatgc agtttttcgt tggtggcgac cacatcgacc
     7621 atcaggttgc cgaacacttt gccggattta atcatcagcc cggtggaaag catattgagc
     7681 accagtttct gcgctgtacc tgctttcatc cgcgaagaac cggtaacaac ttcggcacct
     7741 acaatcggtg taatggcaaa ctcagcggtg gttgaaacgg cgctccccgg attacaggaa
     7801 attcccactg tgcggcagcc gagctggcgt gcgtattcca gtccggcaat cacatacggc
     7861 gtgcgaccgc tggcagcaat gccaaccacc acatcctgtg ccgttaaatt aatatttttc
     7921 agatcgttaa caccaccttc ccggctatct tcagctcctt ccaccgcgtg ctgaatggca
     7981 tattcgcctc cagcaatcaa accaaccacc agacccggtt tcacgccgta ggtgggcgga
     8041 cattcgctgg catccagaat ccccagacga ccggatgttc ccgcaccgag ataaatcaga
     8101 cgaccgccgc cgctgacctg ggcgtggata acatcgatcg ccgcggcgat atccggcagg
     8161 acgcgatcaa cggcaggcgg tacggtttta tcttcatcgt tgataatccg gcacatttcc
     8221 agcgtcgata cgcggtcaat ttcagccgag gcggtgttcg agccttcagt aatcatcttt
     8281 tctagttgca tcggtggtga ccttactatt tacgatcaaa taattgcaga tgtatggaat
     8341 catatatccc gaattgacag acttaaagga atcattgagt ctacactcgc agagtgtgaa
     8401 gagcgtcata agaacttaag cggaattttt gctgccagcc caggagagtg gaagatgttg
     8461 tacctgacga aaatacgcaa tgcggaaagt gaatttaccg ggaacgagca aaaaattgct
     8521 gactttctgc gggccagagt gagcgagctg aaatccgtct cttcacggca gatggcgaaa
     8581 cagctcggta tcagccagtc gagcattgtg aaatttgccc agaagctcgg cgcgcaagga
     8641 tttactgaat tgcgtatggc gttaattggc gaatacagtg ccagccggga gaaaacgaat
     8701 gccacggcgc tgcacctgca cagttcgatt accagtgatg attcgctgga agtgattgcc
     8761 cgcaaactga atcgcgaaaa agagctggcg ctggagcaaa cctgtgcgtt gttcgactac
     8821 gcgcggctgc aaaaaatcat tgaggtcatc agtaaagcac cgtttatcca gataacgggc
     8881 ctgggcggat cggcgctggt ggggcgcgat ctgtcgttca aactgatgaa aattggctat
     8941 cgcgtggcct gcgaagccga cacgcacgta caagccaccg tttcccaggc gttgaaaaaa
     9001 ggcgatgtac aaatcgctat ctcttacagc ggcagtaaaa aagagattgt cttgtgcgca
     9061 gaagcggcac gaaagcaggg ggcaacggtg attgccatta cttctctggc tgactctccg
     9121 ctgcggcggc tggcgcattt cacgctcgat accgtttccg gtgaaaccga atggcgtagc
     9181 tcgtcgatgt ctacccgcac ggcgcaaaat tctgttaccg atttactgtt tgttggtctt
     9241 gtgcaacatc aatgatgtgg cgtcattgaa aatgattcag cgcagcagtg agctaacaca
     9301 acgcctgaaa tgatgcataa agcagcgact ggattgagat tttcctgaat tagtgagctg
     9361 atccgcagca atattttgtt tatcctgtat tttcagaggg aatggagtgt aacgctctgt
     9421 attaacaagg agagcattaa aatgggtaaa ctcacgggca agacagcact gattacgggc
     9481 gcattgcagg gaattggcga aggaattgcc agaacttttg cacgtcatgg cgcgaaccta
     9541 atcttgctgg atatctcccc tgagatcgaa aagctagcgg acgaactgtg tggtcgtggt
     9601 catcgctgta cggcggttgt cgccgatgtg cgtgacccgg cgtcggtagc cgcagctatc
     9661 aaacgcgcga aggaaaaaga agggcgcatt gatatcctgg tgaataacgc aggcgtttgt
     9721 cgtctgggca gtttcctcga tatgagcgat gaagatcgcg atttccatat tgatatcaat
     9781 attaaaggcg tatggaacgt cacgaaggcg gtgctgccgg agatgattgc gcgcaaagat
     9841 ggtcgcattg tgatgatgtc ttcagtcact ggtgatatgg tggccgatcc tggcgaaacg
     9901 gcgtatgcct taacgaaagc ggcgattgtt ggcctgacta aatcgctggc ggtggagtac
     9961 gcgcaatccg gtattcgcgt taacgccatc tgcccgggat acgtccgcac gccaatggcg
    10021 gaaagcattg cccgccagtc gaacccggaa gatccagaat cggtgctgac tgaaatggca
    10081 aaagcaatcc cgctgtgtcg cctcgccgat ccgctggaag tcggcgaact ggcggccttc
    10141 ctcgcatcgg atgaatccag ctatttaacc ggtacacaga atgtgattga tggcggcagc
    10201 acactgccgg agacggttag cgtcggtatc tgattcacct ctgtttcctc cctgcatgtg
    10261 tggggaggat ttcgtcttga actaagttca ccaggctatt tcatttgtca ttttggcccc
    10321 gggcagtgct cgaaatcctc acgtactatg tgtacgctcc ggtttctccg cgctgtccgt
    10381 gtccaaactg cctgcaacaa ttacgcctgt tgaaccaagt tcttattccc tttttaactt
    10441 ccaaatcacc aaacggtata taaaaccgtt actcctttca cgtccgttat aaatatgatg
    10501 gctattagaa agtcattaaa tttataaggg tgcgcaatgg ccgttaactt actgaaaaag
    10561 aactcactcg cgctggtcgc ttctctgctg ctggcgggcc atgtacaggc aacggaactg
    10621 ctgaacagtt cttatgacgt ctcccgcgag ctgtttgccg ccctgaatcc gccgtttgaa
    10681 cagcaatggg caaaagataa cggcggcgac aaactgacga taaaacaatc tcatgccggg
    10741 tcatcaaaac aggcgctggc gattttgcag ggcttaaaag cagacgttgt cacttataac
    10801 caggtgaccg acgtacaaat cctgcacgac aaaggcaagc tgatcccggc tgactggcag
    10861 tcgcgcctgc cgaataatag ctcgccgttc tactccacca tgggcttcct ggtgcgtaag
    10921 ggcaacccga agaatatcca cgactggaac gacctggtgc gctccgacgt gaagctgatt
    10981 ttcccgaacc cgaaaacgtc gggtaacgcg cgttatacct atctggcggc atggggcgca
    11041 gcggataaag ctgacggtgg cgataaagcc aaaaccgaac agtttatgac ccagttcctg
    11101 aaaaacgttg aagtgttcga taccggcggt cgtggcgcaa ccaccacttt tgccgagcgt
    11161 ggcttgggcg atgtgctgat tagctttgaa tcggaagtga acaacatccg taaacagtat
    11221 gaagcacagg gctttgaagt ggtgatcccg aaaaccaata ttctggcgga attcccggtg
    11281 gcgtgggtcg ataaaaatgt gcaggccaac ggtacggaaa aagccgcaaa agcctacctg
    11341 aactggctct acagcccaca ggcgcagacc atcatcaccg actattacta ccgtgtgaat
    11401 aacccggaag tcatggacaa actgaaagat aaattcccgc agaccgaact gttccgcgtg
    11461 gaagacaaat ttggctcatg gccggaagtg atgaaaaccc acttcaccag cggcggcgag
    11521 ttagacaagc tgttagcggc ggggcgtaag taatgtttgc agtttcttca agacgcgtgc
    11581 tgccgggctt tactttaagc cttggcacca gtctgctgtt tgtgtgcctg atattgctgc
    11641 tgccgctctc tgcgctggtg atgcagcttt ctgagatgag ctgggcgcag tactgggagg
    11701 tgatcaccaa cccgcaggtg gtcgcggcct acaaagtgac gctgctgtcg gcgtttgtgg
    11761 catcgatttt taacggcgtt ttcggtctgc tgatggcgtg gattttaaca cgttatcgct
    11821 ttccgggccg cacgctgctt gatgcgctga tggatttacc ctttgcgctg ccaacggcag
    11881 tcgccggttt aacgctggcc tcgctctttt ccgtaaacgg tttttacggt gaatggctgg
    11941 cgaagtttga tatcaaagtc acctatacct ggcttggtat tgcggtggcg atggccttta
    12001 ccagcattcc gtttgtggtg cgtaccgtgc agccggtgct ggaagagtta ggcccggaat
    12061 atgaagaagc ggcggaaacg cttggtgcaa cgcgctggca gagtttctgc aaagtggtgc
    12121 tgccggagct ttctccggcg ctggtggcgg gcgtggcgct gtcgtttacc cgtagtcttg
    12181 gtgaatttgg cgcggtgatt tttatcgccg gaaatatcgc gtggaagacg gaagtgacgt
    12241 cgctgatgat ttttgtgcgc ttacaggagt ttgattaccc ggcagcgagc gcgattgctt
    12301 cggtgatcct cgcggcatct ctgctgctgc tgttctcaat taacactctg caaagtcgct
    12361 ttggtcggcg tgtggtaggt cattaatggc ggaagttacc caattgaagc gttatgacgc
    12421 gcgcccgatt aactggggca aatggtttct gattggcatc gggatgctgg tttcggcgtt
    12481 catcctgctg gtgccgatga tttacatctt cgtgcaggca ttcagcaagg ggctgatgcc
    12541 ggttttacag aatctggccg atccggacat gctgcacgcc atctggctga cggtgatgat
    12601 cgcgctgatt gccgtaccgg taaacctggt gttcggcatt ctgctggcct ggctggtgac
    12661 gcgctttaac ttcccgggac gccagttact gctgacgcta ctggacattc cgtttgctgt
    12721 atcgccggtg gttgccggtc tggtgtatct gctgttctac ggctccaacg gcccgctcgg
    12781 cggctggctg gatgaacata atctgcaaat tatgttctcc tggcccggaa tggtgctggt
    12841 caccatcttt gtgacgtgtc cgtttgtggt gcgcgagctg gtgccggtga tgttaagcca
    12901 gggcagccag gaagacgaag cggcgatttt gcttggcgcg tccggctggc agatgttccg
    12961 tcgcgtcaca ttgccgaata ttcgctgggc gctgctttac ggcgtggtgc tgaccaacgc
    13021 ccgcgcgatt ggcgagtttg gtgcagtgtc ggtggtttcc ggctcgattc gcggcgaaac
    13081 actttcgctg ccgttacaga ttgaattgtt ggagcaggac tacaacaccg tcggctcctt
    13141 taccgccgcc gcgctgttga cgttgatggc gattatcacc ctgtttttaa agagtatgtt
    13201 gcagtggcgc ctggagaatc aggaaaaacg cgcgcagcag gaggagcatc atgagcattg
    13261 agattgccaa tatcaagaag tcttttggtc gcacccaggt gctgaacgat atctcactgg
    13321 atattccttc tggtcagatg gtcgcgttgc tggggccgtc cggttccggg aaaaccacgc
    13381 tgctgcggat tatcgccggg ctggagcatc aaaccagcgg gcatattcgc ttccacggca
    13441 ccgacgtgag tcgcctgcac gcgcgcgatc gtaaagtcgg tttcgtgttc cagcattacg
    13501 cgctgttccg ccatatgacg gtattcgaca atatcacttt tggcctgacg gtgctgccgc
    13561 gtcgcgagcg cccgaatgcc gcagccatca aagcgaaagt gacaaaattg ctggaaatgg
    13621 tccagcttgc gcatctggcg gatcgttatc cggcgcagct ttccggcggc cagaaacagc
    13681 gtgtggcgct ggcgcgcgcg ctggctgtag aaccgcaaat tctgctgctt gatgaaccgt
    13741 tcggcgcgct ggatgcgcag gtgcgtaaag agctgcgtcg ctggctgcgt caactgcatg
    13801 aagagctgaa attcaccagc gtgtttgtga cccacgacca ggaagaggcg accgaagtag
    13861 ccgatcgtgt agtggtgatg agtcagggga atattgaaca ggctgatgcg ccgaatcagg
    13921 tatggcgcga accagcgacc cgttttgtac tcgaatttat gggcgaagtg aaccgcctgc
    13981 agggaaccat tcgcggcggg cagttccatg ttggcgcgca tcgctggccg ctgggctaca
    14041 cacctgcgta tcaggggccg gtggatctct tcctgcgccc gtgggaagtg gatatcagcc
    14101 gccgtaccag tctcgattcg ccgctgccgg tacaggtact ggaagccagc ccgaaaggtc
    14161 actacaccca attagtggtg cagccgctgg ggtggtacaa cgaaccgctg acggtcgtga
    14221 tgcatggcga cgatgccccg cagcatggcg agcgtttatt cgttggtctg caacatgcgc
    14281 ggctgtataa cggcgacgag cgtattgaaa cccgcgatga ggaacttgct ctcgcacaaa
    14341 gcgcctgata ggttgagtga atgttaaacg cccggaggcg cttcccgcga tccgggcttt
    14401 ttaatggcaa ggtttgtaac ctgtaggcct gataagacgc gcaagcgtcg catcaggcaa
    14461 caccacgtat ggatagagat tgtgagtaca ttagaacaaa caataggcaa tacgcctctg
    14521 gtgaagttgc agcgaatggg gccggataac ggcagtgaag tgtggttaaa actggaaggc
    14581 aataacccgg caggttcggt gaaagatcgt gcggcacttt cgatgatcgt cgaggcggaa
    14641 aagcgcgggg aaattaaacc gggtgatgtc ttaatcgaag ccaccagtgg taacaccggc
    14701 attgcgctgg caatgattgc cgcgctgaaa ggctatcgca tgaaattgct gatgcccgac
    14761 aacatgagcc aggaacgccg tgcggcgatg cgtgcttatg gtgcggaact gattcttgtc
    14821 accaaagagc agggcatgga aggtgcgcgc gatctggcgc tggagatggc gaatcgtggc
    14881 gaaggaaagc tgctcgatca gttcaataat cccgataacc cttatgcgca ttacaccacc
    14941 accgggccgg aaatctggca gcaaaccggc gggcgcatca ctcattttgt ctccagcatg
    15001 gggacgaccg gcactatcac cggcgtttca tgctttatgc gcgaacaatc caaaccggtg
    15061 accattgttg gcctgcaacc ggaagaaggc agcagtattc cgggcattcg ccgctggcct
    15121 gcggaatatc tgccggggat tttcaacgct gcgctggtgg atgaggtgct ggatattcat
    15181 cagcgcgatg cggaaaacac catgcgcgaa ctggcggtgc gggaaggaat attctgtggc
    15241 gtcagctcag gcggcgcggt tgctggagca ctgcgggtgg caaaagctaa ccctggtgcg
    15301 gtggtggtag cgatcatctg cgatcgtggc gatcgctacc tctccaccgg agtgtttggg
    15361 gaagagcatt ttagtcaggg ggcagggatt taaggattaa tagcattgga gactgatgac
    15421 aaacgcaaaa ctgcctgatg cgctccgctt atcaggccta caatgtttct gcaatatatt
    15481 gaattagcaa tatttttatg ccggatcagg cgtttacgcc gcatccggca taaacaaagt
    15541 gcactttgtc aataatctga tttgtcggca gttgttgctg ccgacaaatg cagtttttaa
    15601 ttttcgtgtg gaaaagcagt aaggtcatca atcatttttt gaagtattgg tgagtcctga
    15661 ccgtcacccc attgaagaat ttttgcggta agctgatgac gcgctagtgc attgagtccc
    15721 atctttttaa gtttatggga gattttaccc atgactttga tctgaccgaa tgcagtcgcg
    15781 taggtaatca tattgctctg gatagagctc atttcatcgg ctagtaattg cgcacgcaca
    15841 tcctcttcaa gaatactgtc catagcgatg tatttcatac cccacatagt ttcaattaac
    15901 atttgaggga aatcagcaca gtccagaacg gaggctattt tcaagttcgt ttagagcatc
    15961 agcaccttca tcgctaccaa aaggagattc ttcatcccag caacacatat aaagtggatc
    16021 agtaaaatgg cactggaagt ttgggtgtga cgttttaaga tgtaacccaa tctcttcatc
    16081 atcaaaatag tagagatcca tgaagttaaa gtctggagtt tcttgatatc cttttttcat
    16141 ttttgcggca atcaatttac tggcttcttt cagacactgt tcttcattat cgaattcttt
    16201 tgtatggaat ttaccaatac tacctacctt gccatagttg acagctaaac tatccccttc
    16261 gtaatctatc caccaaaatt tattcgattt ttcatcgtga taaataaagc attttttcat
    16321 gttacctcct gtaaatgcct taccaaagcg gattaatttc cttccgcagg caccgtttta
    16381 tccgtctgcg caattaacgc atgtaaaaaa ggctgtgcgt tctcatcgct cttaccgggg
    16441 atcttcacga tatacggctt cccggtaatc gatgacgacg aagctacctt atcaataaac
    16501 tgctcggcag tgtcaatgcg gttacgggta ttgccgagct tcagacgcag gtgggaaacc
    16561 gcttcatcgc aggtatgttc atcaccgttg cgcacaaata tcaaatcctt tttctgtgct
    16621 aatccctcca gcatggcgtt gatgcgggct tcttcatgaa cagttaactt cgcgtaagcg
    16681 gggagtgtca ttagcagcgt aatggccaga caaatgattt tcttcactga attaccttat
    16741 ctttgttttg taatagccga tttgactgcc agaatcaggt aaagtttcgg ttggaatacg
    16801 atgaggatca tcggcaagga gaaaaaatta aatgagtagt ttgttgttgt ttaacgataa
    16861 gagtagggcg ctgcaggcgg atatcgtcgc cgtgcagtcg caggtagtgt acggaagcgt
    16921 aggcaacagc attgcggtgc ctgctataaa acagaacggc ttgaatgtct ttgccgtgcc
    16981 gacggtattg ctgagcaata cgccgcatta tgacactttc tacggtggtg cgattccgga
    17041 cgaatggttt agcggctatt tgcgtgcgct tcaggagcgt gatgcgctgc gccaacttcg
    17101 tgctgtaacc acgggctata tgggaacggc atcgcaaatc aaaatccttg ccgagtggct
    17161 gactgcgcta cgcaaagacc atcctgacct attgatcatg gtcgatccgg tgattggcga
    17221 tattgatagc ggaatttatg tcaaacctga ccttcccgag gcgtatcgac aatatttact
    17281 gccgctggcg cagggaatta cccccaatat ctttgagctg gaaatcctga ccggtaaaaa
    17341 ttgccgcgat ctcgacagtg ccattgctgc cgcaaaaagt ctgctttcag acacattaaa
    17401 atgggtggtg attaccagcg cctccggtaa tgaagaaaat caggagatgc aggtggtggt
    17461 ggtcagtgcc gacagcgtga atgtcatttc ccattcacgg gtaaaaaccg acctgaaagg
    17521 gactggcgac ctgttttgtg ctcagcttat cagtggtttg ctgaaaggga aggcgttaaa
    17581 cgatgcggtg caccgtgcgg ggttgcgcgt actggaagtg atgcgctaca cccagcagca
    17641 tgagagcgat gaattgattt tgccgccgct ggcggaagca taaaaaatgg cgccgatggg
    17701 cgccattttt cactgcggca agaattactt cttgatgcgg ataaccgggg tttcacccac
    17761 ggttacgcta ccggacagtt tgatcagttc tttgatttcg tccatgttgg agataacaac
    17821 cggagtcagg gtagacttgg ctttctcttc cagcagcggc agatcaaatt caatgacagt
    17881 atcgccaact ttcacgcgct gaccttcttc agcaatacgc ttgaagcctt cgcctttcag
    17941 ttcaacggtg tcgataccga agtggacgaa cagttcaacg ccgctatcag attcgataga
    18001 gaacgcgtga ttggtttcaa agattttacc aatggtaccg tctacaggcg cgaccatttt
    18061 gttacccgtt ggtttgatag caataccatc accaacgatt ttttccgcaa aaacgacatc
    18121 cggcacgtct tcgatattga cgatctcgcc agagagcgga gcaatgatct caatagttcc
    18181 ggtatccttt ttgtcgtcgg aaaccagaga tttcagttta tcgaacaaac ccatgatctt
    18241 ctcctaagca gtaaattggg ccgcatctcg tggattagca gattgttttt tcttcaatga
    18301 acttgttaac cagcgtcatt aactcgtccg ttgtcggttg agcaagagcc tgctctgcta
    18361 acaccttcgc atcttcgaag ttcgtgttac ggataatctt cttaatgcgc gggatagaaa
    18421 tggcgctcat agagaattcg tccagaccca tccccagcaa cagaagtgta gcacgttcat
    18481 cgccagcaag ctcaccacac atgccagtcc atttgccttc agcatgagaa gcatcaataa
    18541 cttgcttgat caagttcagc acggacggtg acattggctg gtaaaggtgt gaaatcatat
    18601 cattaccacg gtcaactgcc agagtgtact gcgttaaatc attggtgccg atactaaaga
    18661 aatcaacttc tttggctaaa tgacgtgcaa ttgttgcggc agccggtgtt tccaccatta
    18721 cgccgatttc aattgactcg tcaaacgctt taccttcgtc gcgcagttcc tgtttgtaga
    18781 tttcgatctc tttgcgcagt gcacgcactt cttcaacaga gatgatcatc gggaacataa
    18841 tgcgcaattt accgaaagcc gaggcacgca ggatagcgcg gagctgatcg cgcaggatct
    18901 ctttacgatc catcgcgata cggatagcgc gccagccgag gaacgggttc tcttctttcg
    18961 ggaagttcat gtatggcagc tctttgtcgc cgccgatgtc catggtacga acgataaccg
    19021 cctgcgagcc acacgcttca gccactgctt tgtaagcagc aaactgttct tcttcagtgg
    19081 gcagcgcgtc gcggtccatg aacaggaact cagtacgata cagaccaacg ccttcagcgc
    19141 cgttacgctc tgcaccttca acgtcacgaa ccgtaccaat gttagcgcat acttctacct
    19201 ggtgaccgtc cagcgtaata gctggcagat ctttcagttt agcaagctct gctttttcag
    19261 aagccacttg ctcctgaaca gcgcgcattt tatcaataac ttcgttggtt ggattgacgt
    19321 aaacctgatt atttacggca tccagaatca gatagtcgtc atttttcacc tgagaggtga
    19381 cgctaccggt acccacgata gcaggcagtt ccagagaacg cgccatgata gaggtgtggg
    19441 aagtacggcc acccgcgtcg gtgatgaaac ccagcacctt cttcaggttc agctgtgcgg
    19501 tttcggacgg cgtcaggtca gcggcaacca gaatgacttc atcctgaatg gcgctcaggt
    19561 cgataatctt caggcccagg atgttgcgca gcaggcgctt accgatatca cgtacgtcag
    19621 ccgcacgttc tttcaggtat tcatcatcca gctcttccag ggcagaagcc tgaccttcga
    19681 taacttcatg agcagctgcg tcagctgtca tgtgtttatc tttaatcagg gctatgattt
    19741 cctgctccag ctcctcatct tcgagcagca taatatgccc ttcaaagatg gcttcttttt
    19801 cttcaccgaa cgtttcacca gctttcgttt tgatcgtttc cagctgggct gatgccttgg
    19861 cacgaccgct cagaaaacgt tcaacttcct gatcaacctg gtcggcagaa atttttttcc
    19921 ggtcaatgac aatttcgtct tctttcagaa gcagagcttt accgaaagcg atacccgggg
    19981 atgctaaaat gcctgaaatc ataaccctac cttacttgtg actgattttt aaaagaaccc
    20041 gggaaattac tcgagttccg ccatcagttt aaccagatgt tcaaccgctt tctgctcgtc
    20101 ttcgccttct gcggagatag tcacaacggt accttgagtc aggcccagag tctgcagttt
    20161 aaacaggctt ttcgcgctgg cgcttttgcc gttggaagtc acagtaattt cagaagtgaa
    20221 gcccttagct tcttttacaa actgggcagc agggcgggtg tgcagaccgt tcggagcggt
    20281 aatggtaact tcttgctgga acattgtatt tccccaactt ataggtttag tgttgtggaa
    20341 ctaaagtcta gcctggcggt tcgactttag cctgtattgt tagcgccagt ttttaacaga
    20401 cgcgacgcac gaagagcgca atacagtcca ttgctggcgg gtgttgaccg ctgacgtttc
    20461 gtacgtcatt aaacattatg ccgctaaagg aagaattgaa ccaaatcata aaatcgattc
    20521 agctttggct tttcctgtaa cgattaattt cgcgcatcaa aataattagt ctggttaaat
    20581 accagttccg acaggctgaa tcaatgccag gaggggcgaa agtttgaagc aggccacaaa
    20641 aaagcaccta aaaaggtgct tttttacgca tttttaacaa gctggcatta ctgttgcaat
    20701 tctttctcag tgaagagatc ggcaaacaat gcggtgctta aataacgctc acccgatgat
    20761 ggtagaataa ccacaatatt cttgttggta aagctttcat cttcttgtag tttcaacgct
    20821 gcggcaacag ctgctccaga agagatacct gcaagaatac cttcttcttc cattagacga
    20881 cgggcggtag aaatggcttc ttcattggtg atgccaatga ctttatcgac cagcttgaga
    20941 tcgaggttag ccgggataaa accagcgcca ataccctgaa ttttatgcgg gccaggttta
    21001 atctcttcac ctgccagcac ctgggcgata actggagaat cggttggctc aacggcgaca
    21061 gagataagat cggtcttgcc tttggtgcct ttaatgtagc ggctgacgcc agtcagcgta
    21121 ccgccagtcc caacgccagc aataaataca tcaacctgac cgtcggtatc ttcccagatc
    21181 tccggaccgg tggttttttc gtgaatttca gggtttgccg gattgctgaa ttgttgcagc
    21241 agcaggtatt tctctggatt gctggcgaca atttcttctg ctttttggat tgcgcctttc
    21301 atgcctttag caccttccgt cagcaccagg tttgcaccta acgctttcag cagcttgcgg
    21361 cgttcaatac tcatggtttc tggcatggtc agggtgagtt tgtaaccgcg agcggcagct
    21421 acataggcca gtgcaatccc ggtattaccg ctggtcggtt caaccagttc aacgcctggt
    21481 ttcagcacgc cgcgcttttc ggcatcccaa atcatgttgg caccgatacg gcacttaacg
    21541 ctgaagctgg ggttacgaga ttccaccttc gccagaatgc gtccgttacc gatgcgattc
    21601 aggcgaacca gcggcgtgtg accgatagtc agcgagttat cttcaaaaat cttactcatg
    21661 gcctgtcctt aactgtatga aattgggata caacaggtag catacccgct cagagaatat
    21721 gcggaagtaa ggatttagca tatctatata cagaagggaa ataatgacat gcaagatgga
    21781 ataaggggcg gcataagcca cccctgtttc acacaaacgg tttacaaaat aaccggtaga
    21841 ttgttaccgc cacatcgcgt gtttatcgcg atagcaatcg acccacatcg ccgtcgcgcc
    21901 acaaacggca acgggcatga tgaacagatt aagcagcggg atcatcgtaa acaggctggt
    21961 taaagcacca aactgcatat tggtgatttt gcgtgtgcgc agggcggtgc gcatctcttt
    22021 aaacggcact ttgtggttat cgaaggggta atcgcaatac tggatggcta acatccaggc
    22081 gctaaacagg aaccacagta ccggcgcgac ggtttgccca ataccaggga tgaagtaaag
    22141 aattagtaat acaattgcgc gcggcagata ccagacaaat ttttgccatt cgcgtttcat
    22201 gattcgcggc acatctttca tgataccgaa aatcccggta tctggcggtg tagcgccagt
    22261 caatcgtgct tccagttgtt cagccaataa accgttaaac ggagcggcaa tccagttagc
    22321 aatcgtggag aagaaatagc caaacactaa cagcacagag atgacagcca gaggccacaa
    22381 cagataactc agccattgta gccagtccgg aacgtaactc atgaaagtcg ggatccagac
    22441 atcgagctgt gtaaagagcc accagaatgc gccccccatc aacaaaatat tgaccagcag
    22501 cggtaaaata acgaaacgcc gaatcccagg ttgcgagacg agcttccagc cttgcgcaaa
    22561 atagtaaaaa ccgctgcgtg gggcagatgt gaatgatgaa accataatca ggatgagctc
    22621 cttttgacca atcccaggaa aattctgcgt attttaccgg gtaattgcgc aatggacagt
    22681 taggatatgt tcgaaaaaac agcaaaaagc acgatttcat ctatctttgt gctgtgaaag
    22741 ttaatagtgc acttgcactt gaggtaatcg gcaaatactc ttagtgagta aatgtttgcc
    22801 gtggtggcaa ggtgttagaa caacagagaa tataatgatg caggatttgc gtctgatatt
    22861 aatcattgtt ggcgcgatcg ccataatcgc tttactggta catggtttct ggaccagccg
    22921 taaagaacga tcttctatgt tccgcgatcg gccattaaaa cgaatgaagt caaaacgtga
    22981 cgacgattct tatgacgagg atgtcgaaga tgatgagggc gttggtgagg ttcgtgttca
    23041 ccgcgtgaat catgccccgg ctaacgctca ggagcatgag gctgctcgtc cgtcgccgca
    23101 acaccagtac ctaccgcctt atgcgtctgc gcagccgcgt caaccggtcc agcagccgcc
    23161 tgaagcgcag gtaccgccgc aacatgctcc gcgtccagcg cagccggtgc agcagcctgc
    23221 ctatcagccg cagcctgaac agccgttgca gcagccagtt tcgccacagg tcgcgccagc
    23281 gccgcagcct gtgcattcag caccgcaacc ggcacaacag gctttccagc ctgcagaacc
    23341 cgtagcggca ccacagcctg agcctgtagc ggaaccggct ccagttatgg ataaaccgaa
    23401 gcgcaaagaa gcggtgatta tcatgaacgt cgcggcgcat cacggtagcg agctaaacgg
    23461 tgaactgctt cttaatagca ttcaacaagc gggcttcatt tttggcgata tgaatattta
    23521 ccatcgtcat cttagcccgg atggcagcgg cccggcgtta ttcagcctgg cgaatatggt
    23581 gaaaccggga acctttgatc ctgaaatgaa ggatttcact actccgggtg tcactatctt
    23641 tatgcaggta ccgtcttacg gtgacgagct gcagaacttc aagctgatgc tgcaatctgc
    23701 gcagcatatt gccgatgaag tgggcggtgt cgtgcttgac gatcagcgcc gtatgatgac
    23761 tccgcagaaa ttgcgcgagt accaggacat catccgcgaa gtcaaagacg ccaacgcctg
    23821 atacacttaa ggcaaattaa ctcctcttcg aacccccgct tgtcgggggt ttttagcatt
    23881 gatggtgcga tatggaatca atcgaacaac aactgacaga actgcgaacg acgctttgcc
    23941 atcatgaata tctttatcat gtgatggatg cgccggaaat tcccgacgct gaatacgaca
    24001 ggctgatgcg cgaactgcgc gagctggaaa ccaaacatcc agaactgatt acgcctgatt
    24061 cgcctaccca acgtgtaggt gctgcgccgc tggcagcttt cagccagata cgccatgaag
    24121 taccaatgct gtcactggat aacgtttttg atgaagaaag ctttcttgct ttcaacaaac
    24181 gtgtgcagga ccgtctgaaa aacaacgaga aagtcacctg gtgctgtgag ctgaagctgg
    24241 atggccttgc cgtcagtatt ctgtatgaaa atggcgtttt agtcagtgcc gcgacccgtg
    24301 gcgatggcac caccggggaa gatatcacgt ctaatgtgcg tactattcgc gccattccgc
    24361 tgaagctgca cggagagaat atcccggcgc gtctggaagt gcgtggtgaa gtgttcctgc
    24421 cgcaggcggg gttcgaaaag attaacgaag atgcgcgacg cacgggcggg aaagtttttg
    24481 ctaacccacg taatgcggca gctggttcac tgcgtcagct tgatccgcgt attacagcga
    24541 agcgaccgct cacttttttc tgctatggcg ttggtgttct ggaaggtggc gagctgccgg
    24601 atactcatct tggccgttta ctgcaattta aaaagtgggg ggtgccggtc agcgatcggg
    24661 taacgctttg tgaatcggcg gaagaagtgc tggcgttcta tcacaaagtg gaagaagacc
    24721 gcccgacgct gggctttgat atcgacggcg tggtgattaa ggtcaactca ctggaacagc
    24781 aggagcagct tggctttgtc gcgcgtgccc cgcgctgggc ggttgcgttt aaattcccgg
    24841 cgcaggagca gatgaccttt gtgcgtgacg tcgagtttca ggttgggcgt actggcgcga
    24901 ttacgcctgt tgcgcgtctg gaacctgtcc atgttgctgg cgtgctggtg agtaacgcaa
    24961 ccttacacaa tgcggatgaa atcgaacgtc ttggtttacg cattggcgat aaagtggtga
    25021 ttcgccgcgc tggcgacgtg atcccgcagg tggttaacgt cgtgctttct gaacgcccgg
    25081 aagatacccg tgaggttgta ttcccgacgc attgtccggt atgtggttct gacgttgagc
    25141 gtgtggaagg tgaagcggtt gcccgctgta ccggtggcct gatttgcggt gcgcagcgta
    25201 aagagtcgct gaaacacttt gtttcccgcc gtgcgatgga tgttgacgga atgggcgaca
    25261 aaatcatcga tcagctggtt gaaaaagaat atgtccatac tccggcagat ctgttcaaac
    25321 tcaccgcagg caaactgacc ggactggagc gtatggggcc aaaattggca caaaacgtgg
    25381 ttaacgcgct ggaaaaagcg aaagaaacca cctttgctcg cttcctctat gcacttggca
    25441 tccgtgaagt cggcgaggcc accgcagcag gtctggcggc atatttcggc acgctggaag
    25501 cgctggaagc cgcttcgatt gaagagctgc aaaaggtgcc tgatgttggc attgtcgttg
    25561 catcccacgt tcacaacttc tttgccgaag aaagcaaccg caatgtcatc agcgagctgt
    25621 tggcggaagg tgttcactgg cctgcgccga tcgttatcaa cgcggaagag attgacagcc
    25681 cgtttgctgg taaaaccgtg gtgcttacgg gcagcttaag ccagatgtcg cgtgatgacg
    25741 ctaaagctcg actggtcgaa ctgggcgcga aagtcgcggg cagcgtgtcg aagaaaaccg
    25801 atctggtgat agcgggtgaa gctgcaggat ctaaactggc gaaggcgcag gaactgggca
    25861 ttgaagtcat cgacgaagcg gaaatgctgc gtttgctggg tagctgagat ggaaaaagag
    25921 cagctgattg aaatagccaa tacgataatg ccgtttggca aatacaaagg gcgtcgctta
    25981 attgacctgc cggaggagta tctgctgtgg tttgcccgca aagatgagtt cccggcaggg
    26041 aagctcggtg agctaatgca aatcacgctg ctgattaaaa ccgaggggct gacgcaactg
    26101 gtccagcccc tgaagcgtcc gctttaagct ttatcggcgc tgctttcctg ctgcgcctgt
    26161 aactgttcgg tctggcgttt gtatcgacgc gccagcaccg cacagaccat caattggatc
    26221 tgatggaaaa tcatcagggg cagcaccatc ataccgatca ccgatgtggg gaacagaatg
    26281 tttgccatcg ggatgccatt tgccagactc tttttcgaac cacaaaagac gatagtaatt
    26341 tcatctgcct tattgaagct cagtcggcgt gccataaaga cgttaactac aatcacgata
    26401 gccagaagaa cgcagctgac caccacgata aacagcaatg atccccagcc aactttatgc
    26461 cagataccat taacgacggc ttcgctgaac gctgtataaa ccaccaacag aatggacgtc
    26521 tggtcagttt tcgcaatcca ttttttattg cgcgacaccc agtcaccaat ccacggccgg
    26581 gaaagatgcc ccaacacaaa cggcagcagc agttgcagca taattttacc gacctgctca
    26641 aggctgcccc ttgcaccgtg aacattcatc accagaccaa ccagcaatgg tgaaaggaaa
    26701 atccccagca ggctggatgc tgacgcagaa caaaccgccg ccgcgacgtt accgcccgcc
    26761 attgacgtga aggcgattgc agactgcacg gtagccggga gaatgcacaa gtagagaaaa
    26821 ccggagtaga gcatcgggtc gacatttacc ggtttccacc aggcaaacag tacacccaga
    26881 atcggaaaca gcacgaaggt gctgcacatt acccacaaat gcagtcgcca gtgaccaccg
    26941 ccagcaataa tcgcctcacg cgacaacttc gcgccgtgca taaagaacag cagggcaata
    27001 gctgcggtgg tcagattttc aaagaagggg acgaaatcgc ctctggccgg aaagaaagag
    27061 gccagcaaca ccaccgtgat cagggttaag gtgaaaggat cgaggatacg aaaaagtttc
    27121 ataaagactc cagatgatcg atggttctat tgtgcttctt ccagtttgag aaataaaatt
    27181 gatttattgc atccattcat gaagaaaaca gatgaattat tctttaaaac aattaaaggt
    27241 tttcgtcaca gtagcgcagg agaaaagttt tagtcgtgca ggagagcgta tcggcttgag
    27301 ccagtcggca gtaagtcaca gtgtgaagga gctggaaaat catactggtg ttcgcctgct
    27361 ggacagaacc acgcgtgaag tggtgcttac agatgcaggg cagcagttgg ctttgcgtct
    27421 tgagcgactg ctggatgaac ttaacagcac gttgcgcgat accgggcgta tggggcaaca
    27481 actgagcgga aaagttcggg tcgctgccag ccagaccatt tccgcgcatc ttattccgca
    27541 atgcattgct gaaagccatc gccgctatcc agatattcag tttgtcctgc atgatcgtcc
    27601 gcagcagtgg gtgatggaaa gtattcgcca gggagatgtc gactttggca tcgtcatcga
    27661 tccaggccca gtgggcgatc tgcaatgtga agcgattctt tccgagcctt tctttctgct
    27721 ttgccatcgc gatagcgctt tggccgtgga agattatgta ccctggcagg cattacaggg
    27781 agcgaaattg gtgttgcagg attacgcgtc aggcagccga ccgctgattg acgcagcgct
    27841 ggcgcgcaat ggtattcagg cgaatattgt gcaggagatt ggacatccgg cgacgctgtt
    27901 cccgatggta gccgcaggca tcggcatcag tattcttcct gcactggcgc taccattgcc
    27961 tgaaggtagc ccactggtgg ttaagcgcat tacgccggtg gttgaacgcc agttgatgct
    28021 ggtgcgcagg aagaaccgtt cactttccac cgccgctgaa gcgttatggg acgtggtacg
    28081 agaccagggc aatgcgctaa tggctggtcg ggaaggggat ccgttgtatc agatatagat
    28141 atcgatatga gtcgtttttt caacaggatt tagaagggat acattcagag acaaacgctg
    28201 ctctttctcc tgcgcctttt ctgcctgctg tttctgcaac tgcgccagtt gtgtttccag
    28261 tagggtgatt tgctgttgaa tcaattcggc ctgtttttgc ttgtcttcag cactgccgct
    28321 agtgtctaca atctctttaa tttgttgagt gagtttgatg atttgctgac ttatccgcgc
    28381 gatttgtgaa gcaacatcgc taccttgtga tgccctgtta ctactctgtg tttgtatgga
    28441 aggaattgaa gttgaagaga tagtggccat tgttcagtcc tcgtcactgt acgaggtaat
    28501 atcggtagaa gaacaaggaa cttgagaaga aaaatcaatc ttacaaatcg cagatagcaa
    28561 aaaagcgcct ttagggcgct tttttgcatt ggtgggtcgt gcaggatgac tcgcttcgct
    28621 cgcccttcgg gccgtcgccg cgagcggctc cgttgtttcg ctgcgctcaa ctcaaacctg
    28681 ctgcaggttc gaatctttga gtatcgcaga tagcaaaaaa gcgcctttag ggcgcttttt
    28741 t
//
LOCUS       DADLTY010000035        28364 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.035, whole genome shotgun sequence.
ACCESSION   DADLTY010000035 DADLTY010000000
VERSION     DADLTY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.035"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="JN334_001299"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="JN334_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(1023..2045)
                     /locus_tag="JN334_001300"
     CDS             complement(1023..2045)
                     /locus_tag="JN334_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7437498.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /locus_tag="JN334_001301"
     CDS             complement(2059..3561)
                     /locus_tag="JN334_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7437499.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="JN334_001302"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="JN334_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="JN334_001303"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="JN334_001303"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAY7437500.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="JN334_001304"
     CDS             complement(5877..6218)
                     /locus_tag="JN334_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY7437501.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="JN334_001305"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="JN334_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HAY7437502.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="JN334_001306"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="JN334_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAY7437503.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="JN334_001307"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="JN334_001307"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAY7437504.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="JN334_001308"
     CDS             12897..14240
                     /locus_tag="JN334_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY7437505.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="JN334_001309"
     CDS             complement(14302..14508)
                     /locus_tag="JN334_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HAY7437506.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="JN334_001310"
     CDS             14833..15387
                     /locus_tag="JN334_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY7437507.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..16388)
                     /locus_tag="JN334_001311"
     CDS             complement(15450..16388)
                     /locus_tag="JN334_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437508.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="JN334_001312"
     CDS             complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="JN334_001312"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HAY7437509.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17530..19473
                     /gene="cpdB"
                     /locus_tag="JN334_001313"
     CDS             17530..19473
                     /gene="cpdB"
                     /locus_tag="JN334_001313"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HAY7437510.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19599..19979)
                     /locus_tag="JN334_001314"
     CDS             complement(19599..19979)
                     /locus_tag="JN334_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY7437511.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20068..20928
                     /gene="qorB"
                     /locus_tag="JN334_001315"
     CDS             20068..20928
                     /gene="qorB"
                     /locus_tag="JN334_001315"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY7437512.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21037..22002
                     /locus_tag="JN334_001316"
     CDS             21037..22002
                     /locus_tag="JN334_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7437513.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22110..22772
                     /gene="ytfE"
                     /locus_tag="JN334_001317"
     CDS             22110..22772
                     /gene="ytfE"
                     /locus_tag="JN334_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HAY7437514.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="JN334_001318"
                     /gene_synonym="dagA"
     CDS             complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="JN334_001318"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HAY7437515.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24537..25157)
                     /locus_tag="JN334_001319"
     CDS             complement(24537..25157)
                     /locus_tag="JN334_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7437516.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25376..25921
                     /locus_tag="JN334_001320"
     CDS             25376..25921
                     /locus_tag="JN334_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437517.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25905..26552)
                     /locus_tag="JN334_001321"
     CDS             complement(25905..26552)
                     /locus_tag="JN334_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7437518.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26764..27555
                     /locus_tag="JN334_001322"
     CDS             26764..27555
                     /locus_tag="JN334_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HAY7437519.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27565..>28364
                     /locus_tag="JN334_001323"
     CDS             27565..>28364
                     /locus_tag="JN334_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAY7437520.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6960 a         7551 c         6992 g         6861 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc
    16261 caatcttttt tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt
    16321 gtatgcgatg actttacaaa taactccacc gcaccaataa aattattgtg tctgatcctg
    16381 ggtaacatat ggctacctta ttttatttgg ttattttatg tcgccattaa atatctcatt
    16441 atagaagtaa actatcaaaa aacgctcatt attaaaatgt aacttactag caattgaatt
    16501 tttgcacttt aaatacaatc tctttttatt tataactttt taggttggga taaatcagga
    16561 tggcagaatc aggaaataca ctgtttctgc catctgaatt tagtaaatgg acactctgaa
    16621 ccccggattc aggaacgact cgcgcggagt gtaatccagc ggtttaccct gccagtcgtg
    16681 aacgtgcgct ccggcagctg cagccacagc atgtccggcg gcggtgtccc aaatattcgt
    16741 tggtccgaag cgcgggtaca gctgcgcctg tccttccgcc accaggcaga atttcagcga
    16801 agagccgatg gacgtggtct gatgttcgcc aagctgttgc agatactctt tcagctccgc
    16861 atctgcatgg gaacggctga taaccaccag cggcgggcgc gcatcgcgga cctgaatcag
    16921 cttgcgcaca ccgcactctt ctttccaggc cttgccttct gccgcgctgt acattacgtt
    16981 cattaccggc gcatacacca cacctaaaat cggtttgcca tggtcaatga gcgcaatgtt
    17041 aacggtgaat tcgccattac gtttaataaa ctctttagta ccatccagcg ggtctaccag
    17101 ccagtaacgc tgccagtgct gacggacttc ccaaccggga ggatcttctt cagaaaggac
    17161 cggaatatcc ggtgccagcg tacgtaaacc gtccatgata acggtgtgag cggcaatatc
    17221 cgctgccgtt accggagaat tgtccgcttt gctgacgacg tccatcggtt tcgtcccgtc
    17281 gtagacctgc ataatggcat cgcctgcatt ccgtgcaagc tggcatactt gatctaacat
    17341 ttctccacct cgtctctgtg agcggtgtta acttattgtt ttacttatac cctatcgtta
    17401 atgaatgcgc caactgtgat agtgtcatca ttttcaaagc gtaaaattgt ggcattcttc
    17461 actgttctat aagtaaggcg tttattctcc cttttctttc gtattcccga tgataaaagg
    17521 atgtccctga tgattaagtt tagcgcaacg ctcctggcca cgctgattgc cgccagtgtg
    17581 aatgcagcga cggtcgatct gcgtatcatg gaaaccactg atctgcatag caacatgatg
    17641 gatttcgatt attacaaaga caccgccacg gaaaaattcg gactggtacg tacggcaagc
    17701 ctgattaacg atgcccgcaa tgaagtgaaa aacagcgtac tggtcgataa cggcgatttg
    17761 attcagggga gtccgctggc cgattacatg tcggcgaaag gattaaaagc aggtgatgtt
    17821 catccggttt ataaggcgct gaatacgctg gattatacgg tcggtacact cggcaatcat
    17881 gaatttaact acggtctgga ttacctgaaa aatgcgctgg cgggagcgaa attcccttat
    17941 gtaaatgcca acgtcattga cgccagaacc aaacagccaa tgtttacacc gtatttaatt
    18001 aaagataccg aagtggtcga taaagacgga aaaaaacaga cgctgaagat tggctatatt
    18061 ggcgtcgtgc cgccgcaaat catgggctgg gataaagcta atttatccgg aaaagtgacg
    18121 gtgaatgata ttaccgaaac cgtgcgcaaa tacgtgcctg aaatgcgcga gaaaggtgcc
    18181 gatgtcattg tcgttctggc gcattccggg ctgtctgccg atccgtataa agtaatggcg
    18241 gaaaactcag tttattacct cagtgaaatt ccgggcgtta acgccattat gtttggccat
    18301 gctcacgccg ttttcccggg taaagatttt gctgatatcg aaggggctga tatcaccaaa
    18361 ggcacgctga atggtgttcc ggcggtaatg ccgggcatgt ggggcgatca tcttggggtg
    18421 gtcgacttac aactcagtaa tgacagcggt aaatggcagg tgacgcaggc gaaagcggaa
    18481 gcacggccga tttacgacat cgccaataaa aaatccctcg cggcggaaga cagcaagctg
    18541 gtagaaacac tcaaagccga tcacgatgcc acacgccagt tcgtcagcaa gccaatcggt
    18601 aaatctgccg acaatatgta tagctatctg gcgctggtgc aggacgatcc gaccgtgcaa
    18661 gtcgtgaaca acgcgcaaaa agcgtatgtc gaacattaca ttcagggcga tccggatctg
    18721 gcaaaactgc cggtgctttc agctgccgca ccgtttaaag ttggtggtcg caaaaatgac
    18781 ccggcaagct atgtggaggt ggaaaaaggc cagttgacct tccgtaatgc cgccgatctt
    18841 tatctctacc ccaatacgct gattgtagtg aaagccagcg gtaaagaggt gaaagagtgg
    18901 ctggagtgct ccgccggaca gtttaaccag attgctccta acagcacgaa accacagtca
    18961 ctcatcaact gggatggttt ccgcacttat aactttgatg ttattgatgg tgtgaattat
    19021 cagattgatg ttacccaacc cgcccgctat gacggcgagt gccagatgat taatgccaat
    19081 gcagagcgga ttaagaacct gacctttaat ggcaagccga ttgatccgaa cgctatgttc
    19141 ctggttgcca ccaataacta tcgcgcttac ggcggcaaat ttgccggtac gggcgacagc
    19201 catatcgctt ttgcttcacc ggatgagaac cgctcggtgc tggcagcgtg gattgctgat
    19261 gagtcgaaac gtgcggggga aattcatccg gcggcagata acaactggcg tttagcaccg
    19321 atagctgccg ataagaaact ggatatccgt ttcgaaactt ccccgtcaga taaagccgca
    19381 gcgtttatta aagagaaagg gcagtatccg atgaataaag tcgcgaccga tgatatcggg
    19441 tttgcgattt atcaggtgga tttgagtaag taaaacactt ctttttcggc ctataaatca
    19501 accgcatccg gcatttattg gcggatgcga tgctgccgta tcttatccac cctacaagcc
    19561 atgaaccgta gaccggataa gcgcagcgca tccggcagtt atgccgcacg ttcatcccgc
    19621 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    19681 ctcacctgct cgcccagcgg cgtgaggcta tattccacat gcggcggcac caccggatac
    19741 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    19801 tcactcaccc caccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    19861 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    19921 cactgttccg caaagagatt accctctttc agttgttgtg acaggctaac ctgactcatt
    19981 tcatacttac ctttttgcac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    20041 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    20101 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    20161 gttcgtaatc cggcaaaagc ccaggccctg gcagcacaag gcattaccgt gcgtcaggct
    20221 gactacagcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    20281 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    20341 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    20401 ctcggcctcg ccgatgagca catcgagacg gagaaaatgc tggctgattc tggcatcgtt
    20461 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    20521 ctggaacacg gcgtatttat cggtgcggct ggcgatggca aaatcgcctc agcaacgcgg
    20581 gcagattatg cgacagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    20641 tacgaactgg cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa
    20701 cagagcggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    20761 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    20821 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ctgcccaacg
    20881 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    20941 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    21001 tgaacgtttc ttctgacaga ccaatggatg ccagtaatga ttagcggcgt gctgtacgcc
    21061 ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt gccggaatac
    21121 ccggcgatgt tgcagtcgat ggggcgttat ctggcgttag ggttaattgc gctacccatt
    21181 gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc gggactggct taccgccttg
    21241 atgctcacta tgatgggcaa cctcatttat tacttctgcc ttgccagtgc cattcaacgt
    21301 actggcgcgc ctgtttccac gatgattatc ggcaccctgc cggtggtgat tcctgtcttt
    21361 gccaatctgc tttatagcca gcgcgacggc aaactcgcgt ggggaaaact cgccccggca
    21421 ctgatttgta ttggcatcgg cctggcgagt gtgaatattg ctgagttaaa ccacggactc
    21481 cccgattttg actgggcacg ttatacctct ggcatcgtgc tagcgttagt ttccgtggtc
    21541 tgctgggcat ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc cgacaaacat
    21601 ccgatgatgt gggcgacggc gcaggcgctg gtcacgctgc cggtttctct catcggctat
    21661 ctcgtcgcct gttactggct gaatatacaa acgccggact tctccttacc ttttggcccc
    21721 cgtccgctgg tgtttattag tctgatggtt gcgatagccg tgctttgctc atgggttggc
    21781 gcactctgct ggaacgtcgc cagccagcga ttaccgacag tgattctcgg gccgctgatt
    21841 gttttcgaaa cgctggcagg tttgctgtac acctttttac tccgccagca aatgccgccg
    21901 ctaatgacgc tgagcggtat cgcgctgtta gtgattggcg tggtcatcgc ggtcagagca
    21961 aaaccagaaa aacctttaac tgaatctgtc tcagaaagtt gacatgctgg cagtgagtta
    22021 aataagcctc tgctacgtaa gggttatagc ttttgcctta aaggtgtatt taaaatacat
    22081 cttatcttat taagaatgag gtatcagcta tggcttatcg cgaccaacct ttaggtgaac
    22141 tggcgctctc tatccctcgc gcttcggctc tgtttcgtaa atatgatatg gattactgct
    22201 gtggcggtaa gcagacgctg gcgcgcgcgg cggcacgtaa agaactggat gttgaggtca
    22261 ttgaagctga actggcaaag cttgctgaac aaccgattga gaaagactgg cgtagcgccc
    22321 cgctggcaga aatcattgac catatcatcg tgcgctacca cgatcgtcac cgcgagcagc
    22381 tgccggagct gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac aaaccgagcg
    22441 tgccaaaagg gctgacaaaa tacctgacca tgctgcatga agagctttcc agccacatga
    22501 tgaaagaaga gcagatcctc ttcccgatga tcaaacaagg catgggcagc caggcaatgg
    22561 ggccaatcag cgtaatggaa agcgagcacg atgaagcggg cgaactgctg gaagtgatca
    22621 aacacactac caataacgtc acaccgccgc cagaagcctg caccacctgg aaagcgatgt
    22681 ataacggcat taatgaactg attgatgacc tgatggatca catcagtctg gaaaacaatg
    22741 tactgttccc acgcgcccta gcgggtgagt gatctaaaag ctggatggca ttgcgccatc
    22801 cagcatgata atgcggttat ttccgcagtt cagcagcccg cttcttacca ataaacagcc
    22861 agcccaaccc cagcgcgata aaccacagcg gggtaaccag cagcgcctgg cgagtgtcat
    22921 cttccagtgt cagcaacacg accacgaaca caaagaacgc catacatacc cagcacatca
    22981 gcttgccgag cggcatcttg tagatcgact tttcatgcag atgaggacgc tgtttgcggt
    23041 acaccaggta agagcaaagg ataatcgtcc agacgaacat aaacagaatc gcggaaacgg
    23101 ttgtaatcat cgtgaacgcg ccaatcacgc taggattcac atacaacatc accacgccgc
    23161 cgagcagaca gatacacgag aaggtcaacc ctttcgcggg tactgcgcgc ttagaaagtt
    23221 tagcgaacgc tttcggcgca acaccttcct gcgccagacc aaacagcata cggctggtag
    23281 agaagacacc gctgttagcg gaagacgccg cagaggtcag caccacaaag ttgatcacgc
    23341 tggcagccgc aggcaaacct accaacacaa acagctcaac gaacgggctt ttctccggga
    23401 ctaccgaact ccacggtgtc acggacataa tcacaatcag cgcgaagacg tagaacataa
    23461 tgatacggat cggaatggag ttaatcgcgc gtggcagtga tttctccgga tctttggttt
    23521 ccgcagctgt tgtacctacc agctcaatcc ccacgaaagc gaaaaccgct atctggaatc
    23581 cggcaaagaa gccacttaaa cctttcggga accagccgcc gtcattccac aaatgcgcga
    23641 atgacgcttc cacaccggtc ggtgactgaa agtgcatcgc caccatgacc aggccgacga
    23701 caatcagcga cacgatggcg acgattttga tcatcgcaaa ccagaactcc atctcaccga
    23761 acattttcac ggtggcaagg ttgagcgtca gcagcagcac tatcaccgcc agcgaggcga
    23821 cccagtcgga gagatcgggg aaccagaact gagcataagc cgtgatcgcc accacgtctg
    23881 ccataccggt tacaacccag cagaaccagt aagtccagcc ggtgaaatat cccgcccacg
    23941 gccccagtaa atcggaagcg aagtcactaa aagatttgta ttccagattc gaaagcagca
    24001 attcccccat tgcccgcatc acgaaaaaga gcataaaacc aatgatcata taaacgaaaa
    24061 tgatcgacgg cccggcaagg ctaatcgttt tgccggaccc cataaacaac ccagtaccaa
    24121 tggcacctcc aatggcaata agctgaacat gtcggtttgt gagattgcgc cgtagcgact
    24181 gttcagccgg agcctgatca tcggcaacga cttttacctg atctaccatg ttttttcttc
    24241 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    24301 ctgtatcggg actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat
    24361 ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc
    24421 acaaaaatac acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa
    24481 gggtttattg ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag
    24541 aggatttcca gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcacct
    24601 gcgccgcgct cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc
    24661 ggcatcagag tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg
    24721 ggttcaccac gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa
    24781 tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg
    24841 aattgcaggc cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat
    24901 ttcacgcctt cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg
    24961 tggatttcac gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc
    25021 agcgcatcgg caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca
    25081 ctcagttgtt gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca
    25141 aaagttgggg tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc
    25201 agccacactt ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag
    25261 aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc
    25321 gttagcttat tcataggcta tgattgagga acaagacgtg gagcaggagg aaaccatgcc
    25381 cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg
    25441 cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt
    25501 ggtggtcgga tttctgcttc catctgatga tacgcccaac gcgtcggtgg tgacgcgtga
    25561 agcgcagtta gatattcagt cgcaatcaca accgccgaca gaagaacagc cgcaaactac
    25621 ccaaacgcaa ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt
    25681 cgaacccggt aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt
    25741 ctatgcaatg gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaatgggca
    25801 gatggtgaaa atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg
    25861 gaataatcaa caggtcctgt ttacccgcca gccggatggc agttttattc gtgcgcggta
    25921 gatgaagggt caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct
    25981 tccagtgcct ggggggaaac catactgata tgataaacta ggccaagcgt atgctggttg
    26041 atcaaataat atcccccaag cgcagcaata ttaatattca cctgcaatgg gtcgatacct
    26101 ggcttaaaaa tattgtattt cttcccttca tccaataagg attccattaa ctgcaagtga
    26161 gcatggttaa tttctaagag acgctgtgat tttgcataat gcacaccttt actttgattt
    26221 tcactatgca caatttttaa aaaccaagga ttagcaatat aataatccca tgtaaaatca
    26281 ataattttct caatggcttt ttgtggcgga agatctgaga aatcaagggc cttttctttt
    26341 tgtcgaatat cgttccaggt atattccagc acttcgataa ataaattttc tttattacca
    26401 aagtggtgat aaaccgtctg tttactacac cctgctgcct ttacaatatt atctactctg
    26461 gccccttcat aaccatattc ggcaaattca ttaaccgcac atagcagcaa tttttccttc
    26521 aggctctgta cgttgctggt atcaatatac ataacgcttc cccacgacct cttcgtggtg
    26581 actatcataa cctgatttga tttcagcttc agaaaaaagg gcaaaaactt gtactttcat
    26641 caggttatca aatgtcctgt gatcgccatc gcatatcaac caactagatg gtttatcttt
    26701 ttatcctcca gtcgcaagct aatcatcacc aaggaaacaa aattcaaaaa agagagctat
    26761 tgtatgaaat ccactcgtcc tgttgccgtg attaccggag ccactcgcgg tattggaaag
    26821 ggctgtgcgc aggagcttgc tcgtggcggg tttaacctgc tgattaacga tcgtcccgat
    26881 gccgacagcg tagaaaaact gcatatcaca caacaagaat gtttggcgga aggcgtggaa
    26941 gtgatttgct tccctgctga tgtcggtgat ctatctctgc atgaggagat gctcgacgcg
    27001 gcgcaaaacc agtgggggcg gctggactgt ctgctcaata acgcggggat ttcagtgaaa
    27061 aaacgcggtg acctcctcga cctcgaacca gacagcttcg atcaaaacat tgccattaac
    27121 acccgcgcgc cgttcttcct tgctcaggca ttcagtaagc gcttactggc acagccgaaa
    27181 ccggaagcgg agctgccgca ccgctcgatt attttcgtca gttccatcaa cgccatcatg
    27241 ctggcgatga atcgcggcga atacaccatt gccaaaaccg ccgtttctgc cgctgctcgt
    27301 ctgtttgctg ctcgcctttg taatgaacaa attggcgttt atgaagtgcg ccctggtctc
    27361 atcaaaactg atatgactat tccggcaacg gcttattacg acgaactgat tgccaaaggt
    27421 ctggtgccgt ggggacgttg gggttatccg gctgatattg cttccaccgt ccgcgcgatg
    27481 gcggaaggta aactgattta cacctgtggt caggcggtcg caatcgacgg cggcttaagt
    27541 atgccgcgct tctgaggctg aatcatgaga gatctgttac aacgcccgga tttattcagt
    27601 atcaataccg cgacactggg ctataaaacg ccgctacccg ccattattga tgcctgcgcc
    27661 gcacgcggta ttggcgcaat tgccccctgg cgcagggagt tgcagggtga agatctccag
    27721 caaatcaccc gccagcttgc cgccagtaac atgagcgttt ccggtttgtg ccgtagcact
    27781 tactacaccg cgccgacact ggcggaacgc aaactggcga ttgatgataa ccgccgggcg
    27841 ctggacgacg cagcggtgct taacgccgcc tgttatatgc aggtggttgg cggtctgcca
    27901 acgggcacta aagatctata tgaagcgcga gaacaagtga aacaagggat tcgccagttg
    27961 ctgccacatt cgaaagatgt cggcgtgcca attgcgctgg agccactgca cccgatgaca
    28021 gctgccgatc gctcttgcct gtgcacgtta cgtcaggcgc tggactggtg tgatgaactc
    28081 gatcccgatg gtgagtttgg cctcggcgtg gcggtggatg tttatcacgt ctggtgggat
    28141 ccggatctcg ccagtcagat cctgcgtgcc ggaaaacgca tcctcgcgtt tcatgtttcc
    28201 gactggttaa tgccgaccac tgatctggtc aatgaccgtg gtatgccggg agatggcgtc
    28261 atcaatattc cgtcaattcg tcggctggtt gaaaacgccg gatttaatgg tgctattgaa
    28321 ctggaaatat tctcgcctta ctggtggcag aaagatatta acag
//
LOCUS       DADLTY010000036        28140 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.036, whole genome shotgun sequence.
ACCESSION   DADLTY010000036 DADLTY010000000
VERSION     DADLTY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.036"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..447
                     /locus_tag="JN334_001324"
     CDS             <1..447
                     /locus_tag="JN334_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HAY7437521.1"
                     /translation="ESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGT
                     KAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWE
                     SIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTNDV"
     gene            797..1159
                     /locus_tag="JN334_001325"
     CDS             797..1159
                     /locus_tag="JN334_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY7437522.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            1156..2073
                     /locus_tag="JN334_001326"
     CDS             1156..2073
                     /locus_tag="JN334_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY7437523.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            2086..3351
                     /locus_tag="JN334_001327"
     CDS             2086..3351
                     /locus_tag="JN334_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HAY7437524.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(3418..4041)
                     /locus_tag="JN334_001328"
     CDS             complement(3418..4041)
                     /locus_tag="JN334_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7437525.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSADRPYWITGANCEVVDIGCVFTETT
                     SKATSASALSVTRSGKINANGSIDVATGVSSVNVVATGVYEVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(4789..5133)
                     /locus_tag="JN334_001329"
                     /pseudo
     CDS             complement(4789..5133)
                     /locus_tag="JN334_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head-binding protein"
     gene            5221..5730
                     /locus_tag="JN334_001330"
     CDS             5221..5730
                     /locus_tag="JN334_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7437526.1"
                     /translation="MNWNEVFVLKDDGLLYWSDQYLSRPKMFNISKEKPAGHIFKDKN
                     RKTSYIIIRHQDKIYAAHRIIWEMFYGEIPSGMQIDHKDGNGLNNSIDNLRLVSLGEN
                     LKNKSKYTNNTSGCAGVSWHKTHKKWVAYISDAGKRINLGYFKSLDDAIAVRREAEKT
                     YGYHENHGR"
     gene            5862..6122
                     /locus_tag="JN334_001331"
     CDS             5862..6122
                     /locus_tag="JN334_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HAY7437527.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LDKQNSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSS"
     gene            complement(6212..8209)
                     /locus_tag="JN334_001332"
     CDS             complement(6212..8209)
                     /locus_tag="JN334_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HAY7437528.1"
                     /translation="MAKAWKDVIASPQYQALAPEQKAQAQEQYFNEVVAPQAGESVEQ
                     AKQAFYAAYPLPSVQPVETQQPVAQQQPQQSGFMSDLGEAVKETGRGLVQAGVNVANI
                     PASVADAVTSAAAWAGGKLGIGDGTYQPAPRVTTQGLEQDFGLQQGALTPQTTEGRVF
                     AEALPYLTPAGVERAAAQAPTLAGRIAQGATRLLAENAVGSLAANSAKDDAEALATDL
                     GVGVLAGGAINAAGRGLGAAYRGVRGAIAPEAQQAIRFAEREGVPLHTTDLLQPTSRV
                     GKMAQTTAENIPLAGTSGMRATQQEARSQLVQRFADKFGEYDPSVVIDSLKAKTSGIR
                     RAAGNRLEQVQNAMAGVNIQPARAIQQIDTEISNLQKLGKVADNETISKLQSYRDELV
                     RNAGPDGPVNLDLKQLSDLRSQFRMDVKGERPVLPNRSDAAIQRVYKAMTDDINGAIG
                     QNLGNDTLRKYQQANAVYADEAAKLKNTRLKNVLMKGDLTPEVVNNMLFSKNKSEIKT
                     LYNSVGRVGRAQMRNGIIGKAMEKSGGSPDQFLRQLNILQNQTGITFKGQDAAYLKGL
                     KNYLQSTQQAAKAAVTTPTGQQTIPFIIGYGTAMNPATTGAAVSYGLLTRAYESEPFR
                     NAMLRMANTPRGSTAFEKAMQQAQKAINALTQGAKSDALSE"
     gene            complement(8209..9597)
                     /locus_tag="JN334_001333"
     CDS             complement(8209..9597)
                     /locus_tag="JN334_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HAY7437529.1"
                     /translation="MATWQGSNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFHQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLASSSPEAMQSWLQNNANELARVGVDPNNVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGKLAETIRSNQAGEALT
                     ARGQNITMRGQDLSASTARRGQDLAMQRASTRGIAGNDERTVQLSDGRTVTVGGKLHG
                     AGANAFYEGIDNDGNMVRVPASSIAAPATSAANAQNYEMKKDLDAISGASIDDLGFMT
                     GITGSSGSFALGADIRSRASGVEERKLYNAAQRIMGKMQNQGIAAARDMGASGINTEA
                     EANRYFQGMPKPDFSSPEALQQSMRDIQQYTDNYNQQYNVNVGKSQRQQSQPAQVSQP
                     AASSNFSSLWGD"
     gene            complement(9607..10299)
                     /locus_tag="JN334_001334"
     CDS             complement(9607..10299)
                     /locus_tag="JN334_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HAY7437530.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            complement(10302..10757)
                     /locus_tag="JN334_001335"
     CDS             complement(10302..10757)
                     /locus_tag="JN334_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HAY7437531.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            complement(10757..11605)
                     /locus_tag="JN334_001336"
     CDS             complement(10757..11605)
                     /locus_tag="JN334_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437532.1"
                     /translation="MADSNLNTPVIVQATRLDTSILPRNIFSQSYLLYVIAQSTDVGN
                     VASKANEAGQGAYDAQVRNDEQDVILVDHEIRLASAEAKIQDHETRITNAEAAIVGLD
                     SRLTTAENDIDYLTDEVVSIQNTLSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGTLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATII
                     KIIAEQ"
     gene            complement(11605..13023)
                     /locus_tag="JN334_001337"
     CDS             complement(11605..13023)
                     /locus_tag="JN334_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437533.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEHNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIVHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGCQIRIE"
     gene            complement(13032..13514)
                     /locus_tag="JN334_001338"
     CDS             complement(13032..13514)
                     /locus_tag="JN334_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein p27"
                     /protein_id="HAY7437534.1"
                     /translation="MATVLTKGEIVLFALRKFAIASNASLTDVEPQSIEDGVNDLEDM
                     MSEWMINPGDIGYAFATGDEQPLPDDESGLPRKYKHAVGYQLLLRMLSDYSLEPTPQV
                     LSNAQRSYDALMTDTLVVPSMRRRGDFPVGQGNKYDVFTSDRYYPGDLPLIDGDIPNA
                     "
     gene            complement(13489..13674)
                     /locus_tag="JN334_001339"
     CDS             complement(13489..13674)
                     /locus_tag="JN334_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004940084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437535.1"
                     /translation="MDRMSVFLAADNESGHVQAVIAEKDFQFFERLGFVASVDELKPT
                     SKRGRKAADNGNSTDKG"
     gene            complement(13717..14988)
                     /locus_tag="JN334_001340"
     CDS             complement(13717..14988)
                     /locus_tag="JN334_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head protein"
                     /protein_id="HAY7437536.1"
                     /translation="MPNNLDSNVSQIVLKKFLPGFMSDLVLAKTVDRQLLAGEINSST
                     GDSVSFKRPHQFSSLRTPTGDISGQNKNNLISGKATGRVGNYITVAVEYQQLEEAIKL
                     NQLEEILAPVRQRIVTDLETELAHFMMNNGALSLGSPNTPITKWSDVAQTASFLKDLG
                     VNEGENYAVMDPWSAQRLADAQTGLHASDQLVRTAWENAQIPTNFGGIRALMSNGLAS
                     RTQGAFGGTLTVKTQPTVTYNAVKDSYQFTVTLTGATTSVTGFLKAGDQVKFTNTYWL
                     QQQTKQALYNGATPISFTATVTADADSDSSGDVTVTLSGVPIYDTTNPQYNSVSRQVA
                     AGDAVSVVGTASQTMKPNLFYNKFFCGLGSIPLPKLHSIDSAVATYEGFSIRVHKYAD
                     GDANVQKMRFDLLPAYVCFNPHMGGQFFGNP"
     gene            complement(15000..15884)
                     /locus_tag="JN334_001341"
     CDS             complement(15000..15884)
                     /locus_tag="JN334_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002443472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HAY7437537.1"
                     /translation="MDNELIIDGQVIDMSETQENAEETIIQTESQPENESQDDNGKEM
                     ATDPEKTEETPEDYALRIGDEEIQLNADDDDHIDGQPAPQWVKDLRKGFKETQKENRE
                     LRRQLEEALAKPAEHQQPQPDAIPPKPTLESCDYDEQAFEQALTDWHEKKGRVEQQQQ
                     QKLRQQQEYQQRFQQRVEAHKQRAAKLPVKDYQEMEAIVLSELPPIQQEIIIHCADEG
                     SELLAYGLGKSQQLRQRVAAETDPIRAAFLLGQISKQVSLAPKPKKAIKPEPEVRGGG
                     ADAKQDEFNKLCPGAKIE"
     gene            complement(15898..18024)
                     /locus_tag="JN334_001342"
     CDS             complement(15898..18024)
                     /locus_tag="JN334_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAY7437538.1"
                     /translation="MAETLEKKHERIMLRFDRAYSPQQEVREKCIEATRFARVPGGQW
                     EGATAAGTKLDEQFEKYPKFEINKVATELNRIIAEYRNNRITVKFRPGDREASEELAN
                     KLNGLFRADYEETDGGEACDNAFDDAATGGFGCFRLTSMLVNEYDPMDDRQRIAIEPI
                     YDPSRSVWFDPDAKKYDKSDALWAFCMYSLSPEKYEAEYGKKPPASLDVTSMTSWEYD
                     WFDEDVIYIAKYYEVRKESVDVISYRHPITGEIATYDSDQVEDIEDELAIAGFQEVAR
                     RSVKRRRVYVSVVDGDGFLEKPRRIPGEHIPLIPVYGKRWFIDDIERVEGHIAKAMDP
                     QRLYNLQVSMLADTAAQDPGQIPIVGMEQIRGLEKHWEARNKKRPAFLPLREVRDKSG
                     NIIAGATPAGYTQPAVMNQALAALLQQTSADIQEVTGGSQAMQQMPSNIAQETVNNLM
                     NRADMASFIYLDNMAKSLKRAGEVWLSMAREVYGSEREVRIVNEDGSDDIAVLSAQVV
                     DRQTGAVVALNDLSVGRYDVTVDVGPSYTARRDATVSVLTNVLSSMLPTDPMRPAIQG
                     IILDNIDGEGLDDFKEYNRNQLLISGIAKPRNEKEQQIVQQAQMAAQSQPNPEMVLAQ
                     AQMVAAQAEAQKATNETAQTQIKAFTAQQDAMESQANTVYKLAQARNIDDKAVMEAIR
                     LLKDVAESQQQQFQSPPQSPADLMPS"
     gene            complement(18027..19439)
                     /locus_tag="JN334_001343"
     CDS             complement(18027..19439)
                     /locus_tag="JN334_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAY7437539.1"
                     /translation="MTTINPIFEPFIEAHRYKVAKGGRGSGKSWAIARLLVEAARRQP
                     VRILCARELQNSISDSVIRLLEDTIEREGYSAEFEIQRSMIRHLGTNAEFMFYGIKNN
                     PTKIKSLEGIDICWVEEAEAVTKESWDILIPTIRKPFSEIWVSFNPKNILDDTYQRFV
                     VNPPDDICLLTVNYTDNPHFPEVLRLEMEECKRRNPTLYRHIWLGEPVSASDMAIIKR
                     EWLEAATDAHKKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINE
                     GADWATSLAIEDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDAP
                     YQAGAWADEVVQGDNVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLS
                     FDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCM
                     HCPALVREETEIYVPSSSGW"
     gene            complement(19436..19876)
                     /locus_tag="JN334_001344"
     CDS             complement(19436..19876)
                     /locus_tag="JN334_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAY7437540.1"
                     /translation="MARPTKYQEAYAEQARKLCLLGYTDAELADFFEVSESTINKWKL
                     DYPEFSESIKKGKAVADAEVSDRLYQRAMGFVAPDIDIRVIENRIVETPLEKYYPPDT
                     TAAIFWLKNRQKDKWRDKVDHELTGKDGGAIQIETSPMSTLFGK"
     gene            complement(19879..20121)
                     /locus_tag="JN334_001345"
     CDS             complement(19879..20121)
                     /locus_tag="JN334_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HAY7437541.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITTRTIEAVRKSKEALDLFTTGA"
     gene            complement(20479..21000)
                     /locus_tag="JN334_001346"
     CDS             complement(20479..21000)
                     /locus_tag="JN334_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7437542.1"
                     /translation="MNIVPLNYKGEPIRFNTDGWINATDIAKRFGKRLDHWLSNAETL
                     EYVRALDEVYSGEPSKILHTRDSGYVKTSKARKDRGGGTWLHPKLSVAFARWCDPKFS
                     VWCDLHIDSLLRGELTEQQKYEQACRIRDDRKSKASNGAREMARWRWDKPVIEANVEY
                     WREQLQLTLDIAC"
     gene            complement(21202..21663)
                     /locus_tag="JN334_001347"
     CDS             complement(21202..21663)
                     /locus_tag="JN334_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY7437543.1"
                     /translation="MKLRYKLVISAFLLTLFGSLVWSANHYHNKAIEYKKQRDENAMA
                     LDSAMATISDMQKRQRDVAELDARYTKELADANATIESLRADVSAGRRRLQVAATCAK
                     STTGASGMGDGESPGLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            complement(21647..22123)
                     /locus_tag="JN334_001348"
     CDS             complement(21647..22123)
                     /locus_tag="JN334_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HAY7437544.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIDSLIAKFKEAGGLVNETSL"
     gene            complement(22107..22430)
                     /locus_tag="JN334_001349"
     CDS             complement(22107..22430)
                     /locus_tag="JN334_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HAY7437545.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDLAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            complement(23099..23722)
                     /locus_tag="JN334_001350"
     CDS             complement(23099..23722)
                     /locus_tag="JN334_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7437546.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(23719..23907)
                     /locus_tag="JN334_001351"
     CDS             complement(23719..23907)
                     /locus_tag="JN334_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HAY7437547.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDNKGER
                     HVIVNGVLMVKQGKRGRP"
     gene            complement(23904..24266)
                     /locus_tag="JN334_001352"
     CDS             complement(23904..24266)
                     /locus_tag="JN334_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7437548.1"
                     /translation="MNEYQFVLPYPPSVNTYWRRRGSQYYISDKGQKYRKDVQQIIRQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENV"
     gene            complement(24263..24553)
                     /locus_tag="JN334_001353"
     CDS             complement(24263..24553)
                     /locus_tag="JN334_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY7437549.1"
                     /translation="MVNLRKEARGRECQVRIYGVCNGNPETTVLAHYRMTGICGTGMK
                     PDDLIGAWACSDCHAEIDRRTRILDNKDARLYHLEGVIRTQAILLKEGKIKP"
     gene            complement(24553..25275)
                     /locus_tag="JN334_001354"
     CDS             complement(24553..25275)
                     /locus_tag="JN334_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7437550.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEDCGRINGLGLNQSFCVYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEETAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(25268..25444)
                     /locus_tag="JN334_001355"
     CDS             complement(25268..25444)
                     /locus_tag="JN334_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HAY7437551.1"
                     /translation="MLSPSQSLQYQKESVERALTCANCGQKLHVLEVHVCEHCCAELM
                     SDPNSSMYEEEDDE"
     gene            complement(25437..25796)
                     /locus_tag="JN334_001356"
     CDS             complement(25437..25796)
                     /locus_tag="JN334_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010379765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2591 domain-containing protein"
                     /protein_id="HAY7437552.1"
                     /translation="MDYSQLSDFEINKRVAICCGFTPEDCEIAKLGTSIVGVEWDDET
                     GYAIKTVDYCKSPSDAEPIIVENRIGIIPAPENGLWKAAHRKVGSDSTPYHMTQDENP
                     LRAAMIVFLMMQDANNA"
     gene            complement(25799..25975)
                     /locus_tag="JN334_001357"
     CDS             complement(25799..25975)
                     /locus_tag="JN334_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HAY7437553.1"
                     /translation="MARQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(25942..26115)
                     /locus_tag="JN334_001358"
     CDS             complement(25942..26115)
                     /locus_tag="JN334_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninD"
                     /protein_id="HAY7437554.1"
                     /translation="MMRCYRCGECKEDNRFRPNQPYWNRWCLRCERTPTGVLPLPQEK
                     EDVWRDSDEVSPT"
     gene            complement(26112..26552)
                     /locus_tag="JN334_001359"
     CDS             complement(26112..26552)
                     /locus_tag="JN334_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HAY7437555.1"
                     /translation="MRKLTFELRSPIHQQNAIQAIQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            complement(26533..26787)
                     /locus_tag="JN334_001360"
     CDS             complement(26533..26787)
                     /locus_tag="JN334_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HAY7437556.1"
                     /translation="MPFIKLTMQCSIYQPPSTGVIESTRSAYEPLYVNSDNIETLFDA
                     GFTIVRMASGERFDVIEKPEAILALINPCVQKVSHEETNV"
     gene            complement(26792..27058)
                     /locus_tag="JN334_001361"
     CDS             complement(26792..27058)
                     /locus_tag="JN334_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437557.1"
                     /translation="MDESRKQFEEWFENYTGCDPKNKIYANMVEMYWQAWQASRAAIE
                     IECPGEREREAFSTDFEDGVTFGYNNAISECEGRIRAAGVKVKE"
     gene            complement(27076..27282)
                     /locus_tag="JN334_001362"
     CDS             complement(27076..27282)
                     /locus_tag="JN334_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437558.1"
                     /translation="MVELIFSALRLLGALWMVATFIVVAGCFVRLVGEGKDLVDVLFG
                     SILLWVIIGVMPVAVAKMAWRFVS"
     gene            complement(27359..>28140)
                     /locus_tag="JN334_001363"
     CDS             complement(27359..>28140)
                     /locus_tag="JN334_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional DNA primase/helicase"
                     /protein_id="HAY7437559.1"
                     /translation="GHGKTEVVGHMALEAMRQGVKTCIASLELKPGILLKRLTRQATC
                     CKMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARRRYGIQLFIIDSLMKC
                     GIGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDSEEKPTGKMDVKGSGAITDL
                     TDNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPASVLMLEKQRNGEGWEGGVPL
                     FLDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVTEY"
BASE COUNT         6889 a         6947 c         6296 g         8008 t
ORIGIN      
        1 gagagtaaat caaaccgcgc aattggcgtt gcgctgaatg atactgcatg tcgcgtattg
       61 aaaaaacaaa tcggaaatca tcaccgttgg gtatttgtgt acaaggaaag ctgtaccaaa
      121 ccagacggaa cgaaagcgcc aacagtaagg aagatgcggt atgacgcaaa cacagcctgg
      181 aaagcggcgc tgagacgggc tggtattgat gatttcagat ttcacgactt gagacacacc
      241 tgggcaagtt ggctggttca agccggagtc ccgttatcag tgttacagga aatgggaggc
      301 tgggagtcta tcgaaatggt tcgtcgatat gctcacctcg cacctaatca ccttaccgaa
      361 cacgcacggc aaatagactt gattctgaac ccatcggtcc caaatttgtc ccagtcaaga
      421 aataaggaag gtactaatga tgtgtaactt attgatttaa atggtgccga taataggagt
      481 cgaacctacg accttcgcat tacgaatctg tagcaccaat cataactatc tgttttagca
      541 agcattaacc gcattcacta agatatagtt gatggcacaa acagaaagtt gatgaatgat
      601 gttgccatat atatgtcaca aatacggcac aacgatcttc aaacatatag ccacacattc
      661 acagaagagc acaaagcctt gcaatccagt gcaaagattt gtgtgccaga gtttttctca
      721 tcaactaccg caagtatcga tcgattgaga cttggatgat agacttcatg cctttgatta
      781 ttagctgata gaagaaatgt taaagctatt tgcaaagtac acatctatag gcgtgctgaa
      841 cacacttata cactgggtgg tttttggtgt atgtatctat gccgcgcata ccaatcaggc
      901 tatggcaaac ttcgcaggtt tcgttgtggc tgtgagtttt agcttctttg cgaatgcaaa
      961 attcacattc aaagcatcaa ctacaacgat gcgctacatg ttatatgtcg ggttcatggg
     1021 aacactgagt gctactgttg gatgggttgc tgatagatgt tcacttcctc caattgtcac
     1081 tcttgttacc ttctccgcca tcagcttggt gtgcggtttc gtctattcaa agttcattgt
     1141 ctttagggat gcgaaatgaa gatatctctt gtcgttcctg tcttcaatga agaagaagcg
     1201 ataccaattt tttataaaac ggtacgtgaa ttcgaagagt tgaagccata tgaagtagaa
     1261 attgttttca taaatgacgg aagcaaagac gctacggagt caatcattaa cgctctggct
     1321 gtttctgatc cgctagttgt tccgctgtca tttacacgca actttggtaa agaaccagca
     1381 ttgtttgcag ggttagacca tacaaccggc gatgccgtga ttccaattga tgttgacctg
     1441 caagacccga ttgaggttat tcctcatctt attgaaaagt ggcaggcagg tgctgacatg
     1501 gtgcttgcta aacgttcaga ccgctcaact gatggacggc tgaaacgtaa gacagctgag
     1561 tggttctata aattacacaa caaaataagc accccaaaaa tagaggaaaa tgtcggagat
     1621 tttcgactca tgtctcgtga ggttgtggag aacattaaac tattgcctga gcgcaatctt
     1681 ttcatgaaag gcatactgag ctgggttggt ggtcagacag atgtcgttga atatgtacgc
     1741 acagagcgtg ttgctggcat ctcaaaattt aatggctgga aattatggaa tctggcactg
     1801 gaaggtatca caagtttttc aacctttcct cttcgcgtat ggacttatat aggcttgttt
     1861 gttgcaagca tttcattttt atatggtgca tggatgatta tagacaccat tgtctttggt
     1921 aacccagtac gcgggtatcc ctccatgctt gtatcaatac ttttcctggg tggagtgcaa
     1981 ctgatcggga ttggtgttct cggtgaatat ataggtagaa tttatttaga aacaaaatca
     2041 agaccacggt acttaattaa aagcagaaaa taacaggatg atcttatgaa gatattcata
     2101 atgcgcaatt ggcacaagat atcaattttt atacttgcat ttactttgat ttggcttagg
     2161 cgcattgaca ttttgacaaa tgcccagttt tgggctgagg atgcagtttt ttggtataaa
     2221 gatgcatacg aacaaggttt tttcagttca ctcataaccc caagaaatgg ttattttcaa
     2281 acagtatcaa cactcattgt tggtgcgacg acatttatta atccaattta cgcaccatta
     2341 ttatcaaact tttttggaat aataattaga gctataatta tatggttctt atttaccgat
     2401 agatttaaat tcctaagcac cacatcaaaa atcttcatct ctgtatattt aatttgcatg
     2461 cccgggcttg atgaagtcca agccaatata acaaatgctc actggtattt atcattgtat
     2521 gttgcaatga taataatatc tgaagaatcc aagtcaaaat tatggaaagc acatgattta
     2581 ttttttatag tattatctgg attgagcggt ccatttatta tattcataat tgcatcatct
     2641 gtttttaaat atttacactt atcaaaagga gtaataagca taagaggttt atacttattt
     2701 tatactcgac tgccatattt ggcaatgatc ttgtgtgggc tgatacaggc aacatcaata
     2761 attcttacat ttaatggaac aagatcacat gctccattag gattcagttt tgatgttatg
     2821 tcatctataa tatcatctaa tgtatttctt ttttcatttg tcccttggaa tatcgctaat
     2881 gctggatggg ataatcacct tttatcgtac acactatcaa ctttagtaat tacattatgt
     2941 gttttcattt atattaaagg aaattggcaa atgaaagtgt ttgccaccct tccaatactt
     3001 attgtcgcat tttctatggc aaaacctcaa ctggcagatt ctgttcctca actgccaaca
     3061 cttgcaaccg gaaacgggtc aaggtatttt gtaaatattc atattgcaat tttttcatta
     3121 atatgcgttt gtttgtttca atgcataaag aataaaacct taaaaatatt ctttaaaatc
     3181 tatgtttcag tcatccttat tgcaatgata aaattaaact tttttataac ccctcttcct
     3241 gacatgaact ggtctcaagg agcagaatta ataaataaag caaaacatgg agaagccgtc
     3301 tcatttacag tgctccctcc atggttaact cttgatttaa taaaaaaata aaacttcaca
     3361 ttaataaata acaatgaatc cgcctcccta cgtattcccg tagggaggct gtcgtgttta
     3421 tagtagcctg gtaatgctaa atgcaaacga actggagatt cctgctccgc taagcgtatt
     3481 gcgcagggtc accctaaatg tatctacgcc aatataagta acatcacaac taactgaatc
     3541 aggcgcagat ataggtgtta tctgcacaat ataaccgcta gcaactgctg cgagcggatg
     3601 agaaaagcta acctcataca cacctgtagc aaccacgtta accgatgaaa caccagtagc
     3661 aacgtcgata ctaccattgg cgttaatttt tcccgagcgt gtgactgaca atgcagaagc
     3721 cgatgttgcc ttactggttg tttctgtaaa aacacaccca atgtcaacta cctcgcaatt
     3781 agctcctgtt atccagtatg gtcgatctgc acttggctga taatcccctg cgctctggaa
     3841 cgttgtgccg ttgagtatga ctgtaacttt tcccccacct gagcttgtta cttgaatatt
     3901 agtgtgcgtg tatcttgcac tgctcacgcg atgaaaattt ccaccgttta taaccacagt
     3961 tacagggcgc gtcccagtat tatctatagc aaggtctgcc cccccggcat tagcctcaaa
     4021 gtatgggttg tttaatacca aagcgcacga accgttaagg ccatttatgg ctaattgcgc
     4081 accacctgta ctggaatccc cctgagtacc atttccttca caagtcaggc tattgatagt
     4141 tgtaccagca ccccagcggt ccccgaggat accccattca ctgttctgac tgcacgtaag
     4201 cctatttgca gttatagcgt tcagttcaga atgatttacg gagttggtag cacgccaacc
     4261 gatacgatta ctctgcaagt acacgttcgt catgtcgctc gtgagcatcg agtcagtgcg
     4321 taacccttcc ccgtcagggt ggcggcagat aacaatgttt tcaagacgag tatgtgcttt
     4381 attctgcacc agcataccgt aacttccggg attccctttc agcgtcatgt tgccgatgta
     4441 gtcatatgca gccaatctct gactgttaaa cgctgcgtca cctagcattt ttacgttaaa
     4501 atcagtagct ctacattcca gtattgtctc agccatgttc tcgcctgagt aaaaacagcg
     4561 gacacccgga gcacctacaa agtcggcagg gtaatcatca taattaagta ccagaggagt
     4621 ggtaacatca tacacccctt gctttatttt taatgttcca ccgaaaggcc acgatgtaag
     4681 cgggtcttga tatatttcat tttttattgc aaatagctca tcttgaagat ttttcccgct
     4741 attagtgcca atgtaagtag ccccttcatc agattgaagt aactgcagaa gctgttcagg
     4801 gtcatacttc agcaaattcg gaaaatagaa ctgttgcgaa ccatacgcat catatacagc
     4861 cattgaatgg ccttgctcag ttacaaattt ggcaatctgc ccgttatata ccggataacc
     4921 agcagcgtta atgatgattg gttgagaaac aggaacgtga taaccatctt cgttctccac
     4981 ataaacctga atctggtttt caggatttac agggtcagtg tcaattttac caatataaat
     5041 tttaccattg gctacggctt taaaagaacg agccatagtg aatagttgcg aaggcatgct
     5101 taccacaaca ttggcattta ttgaatctgt cattttaatt tctctatatg ttaagcaagc
     5161 aatattcatt gctagcatgt tttttgtata atgagtgaac tttatttaaa ggtggttaac
     5221 atgaactgga acgaagtatt cgtattaaaa gacgatggac tcctgtactg gagtgaccaa
     5281 tatctctcaa gacccaaaat gttcaatatc tctaaagaaa agcctgcagg acatatattc
     5341 aaggacaaga acaggaaaac atcttatata attatcaggc atcaagataa aatatacgcc
     5401 gctcatagga taatatggga aatgttttat ggtgaaatcc cttcaggaat gcaaattgac
     5461 cataaagatg gtaatgggtt aaataattca atagacaacc tgcgtctagt tagtctcggt
     5521 gaaaacctaa aaaacaaatc taaatacaca aacaatacat ctgggtgcgc aggtgtttca
     5581 tggcataaga cccataaaaa gtgggtggca tatattagcg acgcagggaa gcgcattaat
     5641 ctcggatact ttaagagtct cgatgatgca attgctgtaa ggcgtgaggc tgaaaagacc
     5701 tatggttatc atgagaatca tggtaggtga tatctgacat ttcattgctc cagacgaatg
     5761 atatgatgca accatgatgt gattgcatac cgaaatggta ctattgagta tttatccagt
     5821 agattacgat gccattccac ccaactggtg aggcatcaag gatgtacagc aaatacgacg
     5881 aggcgcagtt tcacttgaga cttccgcatg aactccacgc gaaaattaag cagcgtgcga
     5941 agatgaataa caggtctctg aactcagaga taattgcagc gattgaagaa tcactggata
     6001 aacaaaactc tgcatcagtt tacattgacg atgcagagcg tatggcagaa caacaatccg
     6061 atatggttaa gaaaattgtc tttgatacgc tcaaagagct atataaaaaa gacagcagct
     6121 aaccatcagt tacggaggat ttatgcaaag agatatgctg aatattgcgt tctacatatt
     6181 tggtttttgc acgttcctgg tgtttgcgaa gctattctga caacgcatca gacttggcac
     6241 cctgagtcag ggcgttaatg gccttttgcg cctgctgcat ggctttctca aacgctgttg
     6301 atccgcgtgg ggtgtttgcc attcggagca ttgcatttct gaatggttcg ctctcatagg
     6361 cgcgagtaag aagtccgtag cttactgctg cgccagttgt cgccgggttc attgccgtcc
     6421 catacccgat aatgaacggg atggtttgct gccctgttgg tgttgttact gccgcttttg
     6481 ctgcctgctg agtggactga aggtaatttt tcaatccttt cagataagca gcgtcctgcc
     6541 ccttaaatgt gatgccagtc tggttttgca ggatgttaag ctgccgaagg aactggtcag
     6601 gggatccgcc agatttctcc atcgcctttc caatgatgcc attgcgcatt tgcgccctgc
     6661 caacacgacc aactgagtta tacagcgtct taatttccga tttgttcttg ctgaatagca
     6721 tgttgttgac aacttccggc gtcaggtcgc ctttcatgag aacattcttc agcctggtat
     6781 tctttagttt cgccgcttcg tcagcgtaga cggcattggc ctgctgatat ttacggagag
     6841 tatcgttgcc aagattctga ccaatggcac cattgatatc gtcggtcatt gccttgtaaa
     6901 cgcgctgaat ggcagcatcg gaacggtttg gtaacactgg ccgctccccc ttcacgtcca
     6961 ttctgaactg gctgcgcaga tcgcttaatt gcttcaaatc cagattaacc ggaccatcag
     7021 ggccagcatt gcgaacaagc tcatcacgat atgactgaag ttttgaaatt gtctcgttat
     7081 cagctacctt accaagcttc tgcagattag atatttctgt atcaatctgc tgaattgctc
     7141 gcgcaggctg aatgtttact ccagccatag cattctgaac ctgctccagt cgattaccgg
     7201 cggcacgacg aattcctgat gttttcgctt taaggctgtc aataacaaca gatggatcat
     7261 actcaccgaa tttatcagca aatctctgca ccaactggct tctcgcttcc tgttgcgttg
     7321 ctctcattcc gcttgtgcca gccagaggga tattttctgc tgtagtctgc gccatttttc
     7381 cgacgcggga agtgggttgt aacaggtctg tggtgtgcag aggaacgcct tcacgctctg
     7441 caaatctgat agcttgctgt gcttctggcg cgatagcacc acgaacgcca cgataagcag
     7501 cacctaatcc acgtccggca gcgttaatag caccgccagc cagcacacca acgcctaaat
     7561 cggtggcgag tgcttccgca tcatctttcg cactatttgc agcaagtgat ccaactgcgt
     7621 tttcagcgag aaggcgagtt gccccctgag caattcgacc agcaagtgtt ggtgcctgtg
     7681 ctgccgctct ctcaacgcca gcaggagtga ggtaaggcaa tgcttcagca aatacccttc
     7741 cctctgtcgt ttgtggagtc agcgcacctt gctgaaggcc aaagtcctgc tctaatccct
     7801 gcgttgttac tcgtggcgct ggttgatatg tcccatcgcc aatgccgagt ttaccgccag
     7861 cccaagccgc cgcgctcgtt acagcatcgg caactgatgc aggtatattt gccacgttca
     7921 cgccagcctg caccagtccg cgaccagtct cttttactgc ttcgccaaga tcagacataa
     7981 atccactttg ctgtggttgt tgctgtgcta ctggttgttg tgtctccact ggctgcacag
     8041 atggcaatgg ataggcagca taaaaagcct gcttagcctg ctctacactt tctccggctt
     8101 gcggggccac gacttcattg aagtattgct cctgagcctg cgctttttgt tctggtgcta
     8161 acgcctgata ctgtggagaa gcgataacat ctttccatgc tttagccatt aatcacccca
     8221 tagtgaagaa aagttactgc tggctgctgg ctgtgatacc tgtgcaggtt gagattgctg
     8281 ccgctgagat ttaccaacat taacgttata ttgctggttg taattgtcgg tatattgctg
     8341 aatgtcgcgc attgattgtt gtagtgcttc agggcttgag aaatcaggct ttggcatacc
     8401 ctgaaaatac ctattggcct ctgcttcggt gttgatgcca gatgccccca tatctcgggc
     8461 tgccgcaatc ccctgatttt gcattttgcc catgattcgc tgtgcagcgt tgtatagttt
     8521 cctctcctca acaccagatg cccggctacg aatatctgca ccaagagcaa aagaacctga
     8581 agaacctgta atacctgtca tgaagccaag atcgtcaatt gatgcgccag aaattgcatc
     8641 aagatctttc ttcatttcgt aattctgcgc attagctgcc gatgtagccg gagcagcaat
     8701 agaacttgcg gggacgcgaa ccatattccc atcgttgtcg atgccttcgt agaacgcatt
     8761 agccccagcg ccatgaagct tcccgcctac cgttacagtt ctgccatctg ataactgaac
     8821 tgtacgctca tcattcccag cgattcctct tgttgacgct cgctgcattg ccaaatcctg
     8881 acctcgtcgc gcagtagaag cagataagtc ctgaccgcgc atcgtgatgt tctggcctcg
     8941 tgctgttagc gcctcgccag cctgattgct gcggattgtc tctgcaagtt tcccgcgatc
     9001 aatctcacga ccagccatct tgtcctgaac attgaagtaa tcaattggac caagagcagc
     9061 cattccaagg tgatcaacaa actcaccaaa tcctgaaggg ttctgctgat acatctgagc
     9121 aacgttatta gggtcaacac cgacgcgcgc cagctcattg gcgttgtttt gcagccatga
     9181 ttgcattgct tctggagaag atgacgcaag acgtgcgcca gccgctaatg taccgataga
     9241 attacgctgg tcttcatcaa tgaatcccat gcctttacga acggattcaa tctggtctgg
     9301 atattgagta gccaactgac gcaaagcacc gcgatcacca gaagcataag cattagcgta
     9361 tgcctgctga aattctttct gccgctgagc ctgcttttcc tgatgaaaaa cacccgcaat
     9421 acctgaaaga ccttgcaaag cagtcagccc aacattgtta gcacctgaac gctcaatatc
     9481 attgttctgc ctgataagct gaagcgtatt gccgatgtca tttacgctcg gagcgtttga
     9541 gttgacgccg ccgataccag ccaacaatcc gccatttgat ccttgccaag tagccatgat
     9601 taccccttaa aacaacgagc caagcaatcc gataccagca ccaatgccag cgccccaagg
     9661 tgttgatgtt cccaaaaggc tggcaagacc tgcaccggca atcgcaccag acgttccgcc
     9721 actaattgct gtctgaagac ttgatggttt gttggcatta gcagcggcaa gagctgcgct
     9781 ttgctgtgca atgctgctca tgttgttggc gtacgtctgc ccggcgtttg cctgaccttg
     9841 cagcgcacca agcccaacgt ttgccagatt gttgtaattg ctcatctggt ttgataacca
     9901 agactgaccg agtgtcggcg cgatcgtagc cagttgattg cttgtggctg tcgaaccaag
     9961 tccacccgtc gcctccgcag cagcaagact ctggtaacgc gcctgacctg caaggtcttt
    10021 atactgctga gagttgtaat actgattaag tgcctgcccc tgaccttcta aactggaaag
    10081 attctgcaac tggttaacat actgctccgc aagaggcgtg aacggagcaa ggtttttcat
    10141 gatcgtctgc cactgctgat tttgcaggtc tgctgcatac ttctgagctt ctgcggcata
    10201 ctttgcgctt ttatcagaac ttccaccttt cccacccttt tcagggcaat aaggttcctc
    10261 gccgcgcagt tttctgccca gcttaaatgc atataacatg gctatctccc gtgattcagg
    10321 aagtcgatta gttcttcgcg tgttgcgctg taaaaagtca cgtcatccac gcctttgaag
    10381 tatttcttga tggttcctac tcgcttaagg ccaatcattg cgcagtacat ctgaccgtgg
    10441 cggaatttgc gcgcagcgaa cgatgtgacg cactgaacgg tggtgttagt cagaatgtat
    10501 cgccagaacg ccagcccgat ttccttgctg aatccacgaa cctctggcag gtacatggcg
    10561 tggcaatcaa aggtcaaagg ctgaatctcc tgatagtaaa caatgccgcc gaactgcccg
    10621 tgcacgttca cctcgaagta acggcattca ggcttgtagt cgtatccatc accgttgttg
    10681 ctcccggcga taatgtcggg gtgatttccc acggcttcta tcaggtcgat gtttcgcgtt
    10741 ggtttgaact gaatcatcac tgctccgcga ttatcttgat gattgtggca gtaaacgccg
    10801 caccatttga ctgaatggtt aacgtactgc catttgtggc aagaaagccg tctttatcca
    10861 cgctgaagaa cgtagctaac aggatgttgt cggttgttgt cgccgcatta cgactgctta
    10921 ccagtgtgtc aggaacagag ccggaaaagg ttagctgcat tgacctgttt gcggttccac
    10981 tgggccacgt cccgacaata gacagcttga agaacagcgt tttgttctcg ttgaacacaa
    11041 ccatcttgtt gttaacggtg tcgaagaatg gcgctaatgt tcctgatgac ggcgtgagcg
    11101 ttttcagcag actgacaagg ttggtcggcg ctgtcgggat ggttactgat acgcccgagt
    11161 aaacaacttc tgacttcttg cgcgtagtgg catactccag agcatcgatg cgcgtttcat
    11221 ggtctgaaag cgtgttttga atggagacaa cttcatccgt cagataatca atatcgtttt
    11281 ctgctgtcgt taatcgtgaa tcaaggccga ctatcgccgc ttctgcgtta gtgatccttg
    11341 tttcgtggtc ctgaatctta gcttcagctg atgccagtcg aatttcgtga tcgaccagaa
    11401 tcacatcctg ctcatcgttc ctgacctgtg catcataagc gccctgtccg gcctcgttgg
    11461 ccttgctagc cacgttacca acatcagtac tctgtgcgat aacgtacagc agatatgact
    11521 gcgagaagat attgcgtgga aggattgatg tatcgagccg cgtcgcctga acaataacag
    11581 gtgtattgag attcgaatcc gccattactc aatccttatc tggcagccag acagagtgac
    11641 aggtgacttc gtgataacgc gcaatttgaa gccgacattt ttcctgatgc gccctactcg
    11701 cttccacaaa acgcgtttgt cgtaaacgaa cggttcattc tgctcaatca tctgctcacg
    11761 cccgtaattg atgccgtcag tggttgcaga gagaaaaagg cggtcagcat actgcgcaac
    11821 gccagttgac gattcaacct caaggtcgaa cactctggcg ttatccgctt tgaacagtgg
    11881 agtaaacagc aggtgttcct gttgcttgtc gtactggctg ctgatgtcga attgcaattt
    11941 cccggtcacg gactccagct tatcgccgca cgttatctga ttgccttcgt aaatgaagtc
    12001 gatagcgcgg tacacatcgt catacaggcc tgttttcaac acacaccatt gcggaccatt
    12061 ggcgcttgaa gatgcgtcgt aaacaagaac atggcgcggc aggtgaacaa tcagcaactc
    12121 atgagcatca aaccgcaacg attccatcac gccatcagcc agttcatcag cagtgtagga
    12181 gcgtagtatt ttctcaatgc tcgcgctggc gattggtgat acctgaccgg agccgatgat
    12241 gtatacagac ggcgcacctg ttgccggatt gctgatgaat gcataagaat cagcaaacgg
    12301 cgttttgcag taagtcccgg caatgccttt ctgcaccatc agcgatggct gtgcgacata
    12361 cagagcagcc ccaacagtgg ttgcaccagt cagggagaaa tattcaatcg tcgacgaacc
    12421 aaagcagact atgaagtctc gccatgtacc tatgccgatg atgccgtcag gctgcgactc
    12481 tgcacgatat tgtgcgctgt atcggtcagg atgcgattcg tcttcaaggt cagtgataaa
    12541 ccatgaatca gttccgtctt ttgaccacgc ataacgccca cgtaagcgcg taatgtcgcg
    12601 aactgaacct aactcgtact gtgtgaatcc gctgtctgta ggccagtttg agacggtttt
    12661 aaccgtgcca tcatagcgat actcgaccag ttgaccatta acgcctaccg cctgtgatgt
    12721 tcgaccatgc gccattgata cgcgaccact tccggcaaca tcaccaacct cgctttcgcc
    12781 cttatacagc ttgccaccac acacgcgata aacagcattc tgcgccatgt tgtgctcgac
    12841 gcctcgcgat acgccgttca catcagaacg tttggcaatg cccgggaatg agcgaagata
    12901 tccgctgctg ttcaggattt ctttgggtgt agccagcata ttcactggca gatagtcgat
    12961 atagtcggcg tttctaaagt ctttgccgac acctttcata agcggaagtt gctgaatagg
    13021 catttattca cctatgcgtt tgggatatcg ccatcaatca gagggagatc gcctggataa
    13081 tatcggtcag atgtgaacac gtcatattta ttaccctgcc ctacaggaaa atctccacgt
    13141 cgtcgcattg aaggaacaac cagagtgtcg gtcatcaagg catcatatga gcgttgggcg
    13201 ttactgagaa cttgcggagt tggttcaagg ctgtaatcag atagcattct cagcaataac
    13261 tgatagccta ctgcgtgttt gtattttctt ggaagacctg actcatcatc tggtaatggc
    13321 tgctcatctc cagttgcgaa agcgtaacca atgtcgccgg ggttaatcat ccactcggac
    13381 atcatatctt ccagatcatt tacaccatct tcaattgatt gcggctcaac atcagtaagc
    13441 gatgcattag aagcaatagc aaacttacga agcgcaaaaa ggacgatctc accctttgtc
    13501 agtactgttg ccattgtccg ccgccttacg acctcgctta ctggtcggtt tcaattcatc
    13561 aactgaggca acaaagccca acctttcgaa aaactggaag tctttttctg cgataacggc
    13621 ctgtacatgc ccggattcgt tatctgcggc aaggaataca ctcatgcgat ccatattgtt
    13681 tccttaaaac ataaaagggg cgtaagcccc ttgttattac ggattaccga agaactgacc
    13741 gcccatgtga gggttaaagc acacatatgc aggcagtaag tcgaagcgca ttttttgcac
    13801 gttggcatcg ccatctgcgt atttatgtac gcggatggag aaaccttcat atgttgcaac
    13861 agcagaatca atactgtgca gtttcggcag tgggatagag ccaagtccac agaagaactt
    13921 gttatagaac aggtttggct tcattgtctg gctagcagtg cctactacag atacggcatc
    13981 gcctgccgct acctgacgac ttacagagtt gtactgcggg tttgtagtgt cataaatcgg
    14041 aacaccagaa agcgtaaccg tcacatcgcc actgctgtct gaatcagcat cagcagtaac
    14101 cgttgcagtg aagctaattg gtgtggctcc gttatacaac gcctgtttgg tctgctgttg
    14161 cagccagtag gtattggtga atttaacctg atcaccagct ttcagaaaac ctgtaacgct
    14221 ggttgtcgct ccggtcaatg ttacagtgaa ctggtatgag tctttaactg cgttataggt
    14281 aacagttggc tgtgttttga ctgtcagtgt tccgccaaat gccccctgcg tacgagaggc
    14341 aagcccatta gacatcagtg cgcgaatgcc gccaaaattg gttgggatct gtgcattctc
    14401 ccatgcagta cgaaccaatt gatctgaagc gtgcaaacca gtctgcgcat cagcaagtcg
    14461 ctgtgcagac catggatcca ttacagcata gttttcacct tcattaacgc cgaggtcttt
    14521 caggaaagat gccgtctgcg caacatcaga ccatttggtg attggagtat tggggctacc
    14581 aagtgacaac gcaccgttat tcatcatgaa gtgagcaagc tctgtttcaa ggtcggtaac
    14641 gattcgctgg cgaaccggcg cgagaatttc ctccagttgg ttaagcttga tcgcttcctc
    14701 cagttgctga tattcaacag caacagtgat gtagttacct acacgccccg tagctttacc
    14761 tgagatcagg ttgtttttat tttgccctga aatatcacca gtgggagtac ggagggatga
    14821 gaattgatgc ggacgtttaa agctaacgct atcgccagtg ctggagttga tttcacctgc
    14881 cagcaactga cggtctacgg ttttcgccag aactaaatct gacataaaac ccggaaggaa
    14941 ttttttcaga acgatttgac tgacgttact gtcgagattg ttaggcattt atcttttcct
    15001 tattcgattt ttgcgccggg gcataatttg ttgaattcgt cttgtttcgc atcagcaccg
    15061 ccaccacgta cttccggctc tggcttgatg gctttctttg gttttggagc aaggcttacc
    15121 tgtttgctaa tctgccccaa gaggaatgct gcgcgaattg gatctgtctc agcggctaca
    15181 cgctggcgta attgctggct cttacctaag ccataggcta gtagttcaga gccttcgtct
    15241 gcacagtgaa tgatgatttc ctgctgaatt ggtggtagct cactaagaac aatggcctcc
    15301 atttcctgat aatctttcac aggaagtttg gctgcccgtt gtttatgcgc ttctaccctt
    15361 tgctggaaac gctgttggta ttcctgttgc tgacgtagtt tttgttgctg ctgctgttcg
    15421 acacggcctt ttttctcatg ccaatcagtc aatgcctgtt caaacgcctg ttcgtcataa
    15481 tcacacgact caagagtcgg ttttggtgga atagcgtctg gttgtggttg ctgatgttcc
    15541 gcaggcttgg ctaatgcttc ctcaagctgg cggcgcaact cacggttttc tttctgtgtt
    15601 tctttgaagc ctttgcgaag atctttcacc cattgcggtg caggttgccc gtcaatgtga
    15661 tcatcatcgt cagcgttaag ctgaatttct tcatcaccaa tacgcaaggc gtaatcttct
    15721 ggtgtctctt cggttttttc aggatcagtt gccatctctt ttccgttgtc atcctggctt
    15781 tcattctcag gctgtgactc tgtttggatg atggtttctt ctgcattttc ctgtgtttca
    15841 gacatgtcaa taacctgacc gtcgatgatc agttcgttat ccattgatta ctcctggtta
    15901 actcggcatt aagtctgccg gtgactgtgg tggtgactgg aattgctgtt gctgtgactc
    15961 ggcgacatct ttcagaaggc gtattgcctc catcactgct ttgtcatcga tgtttctggc
    16021 ttgagccagt ttatagacag tgtttgcctg actctccatc gcatcctgct gggcagtaaa
    16081 tgctttgatt tgagtttgag cagtttcgtt agttgctttt tgcgcttctg cctgcgctgc
    16141 taccatttgc gcctgagcga gaaccatttc aggatttggc tggctttgtg ctgccatttg
    16201 cgcctgttga acaatctgct gctctttctc attgcgtggt tttgcaatgc cagatatcag
    16261 cagttggttt cggttgtact ctttgaagtc atcaaggcct tcgccatcga tattgtccag
    16321 aataataccc tgaattgccg ggcgcattgg gtctgttgga agcatagagc taaggacatt
    16381 tgtcagtaca gaaaccgttg catcacgtcg tgctgtgtag cttggtccaa catcaaccgt
    16441 cacatcgtat cgaccgacag aaaggtcatt taacgcaaca acagcccctg tttgcctgtc
    16501 aacaacctgt gcactcagga cagcgatatc atcacttcca tcttcgttaa cgatgcgcac
    16561 ttcacgctct gaaccgtaca cttcacgagc cattgacagc catacttcac cagcgcgttt
    16621 aagacttttc gccatattgt ccagatagat aaacgaagcc atatctgctc tgttcatcaa
    16681 gttgttaacc gtttcctgag caatattact tggcatctgc tgcatggcct gactgccgcc
    16741 tgtaacctcc tgaatatcag cactggtttg ctgtagtaat gcagccaatg cctgattcat
    16801 aaccgcaggc tgtgtatatc ctgccggggt agctccagcg ataatgttgc cagatttatc
    16861 tctcacttcg cgcaacggca agaacgctgg tcgtttcttg ttgcgagcct cccagtgctt
    16921 ctcaagtcca cgaatttgct ccatgccaac tatagggatc tgaccggggt cttgcgctgc
    16981 agtatcagcc agcattgaaa cctgaaggtt gtacaaacgc tgtggatcca ttgcttttgc
    17041 aatgtgccct tcgacacgct caatgtcatc aatgaaccag cgttttccat aaaccgggat
    17101 gagggggata tgctcaccag gaatacgacg aggtttctca aggaaaccat caccatccac
    17161 tacggataca tacacacgac ggcgcttcac tgagcgcctt gccacttcct gaaatccagc
    17221 tattgccagt tcatcttcaa tatcttcaac ctgatcactg tcgtatgttg caatctctcc
    17281 agtgattgga tgtcgataac tgatgacgtc aacagactct ttacgaactt cgtaatactt
    17341 cgctatgtaa ataacatctt catcaaacca gtcatattcc caactggtca tagacgttac
    17401 atccagagaa gcaggaggtt tctttccgta ttcagcctca tatttttcag gtgacaacga
    17461 atacatgcag aacgcccaca acgcgtcaga tttgtcgtac ttcttagcgt cagggtcaaa
    17521 ccacacagag cgcgacgggt cgtatattgg ttcaatagca atacgctgac gatcgtccat
    17581 ggggtcgtat tcattgacca gcatcgacgt caaacggaag caaccgaaac caccagtagc
    17641 agcgtcgtca aatgcattat cgcaagcctc accgccatca gtttcttcgt agtcagcacg
    17701 gaacagacca tttaatttat tggctaactc ttcgcttgcc tctctgtcac caggacgaaa
    17761 cttaacggtg attctgttat tgcggtattc tgcaatgatg cggttaagtt cagttgcaac
    17821 cttattgatt tcaaacttag gatacttctc gaactgctca tcaagcttag ttccagccgc
    17881 cgttgctcct tcccattgac ctccggggac acgagcaaac ctcgtagctt caatgcactt
    17941 ttcgcgcact tcctgctgtg gagaataggc gcggtcaaac ctgagcatga tccgctcatg
    18001 ttttttctct aatgtctctg ccatgtttac caaccggagg atgagggaac gtatatttca
    18061 gtttcttcgc ggaccaatgc cgggcaatgc atacacatca tcagcgcatc agccaggtta
    18121 ggagatggaa taccgagctt ctgcttcatt tcgaccttag tcataagctc cagcttcccg
    18181 ttattattga atttgcgctg aatctgcgtc agttctgcaa acagcttctc cagcatcttc
    18241 tcgcctatcg cttctttgtc gaagctcagc atgtcgtcgg ggtctgcata ctcaccgtgg
    18301 acaaccgccc gatatgtcag atacagcctg tcagccagcg cgtaatagaa ttgcgctcgc
    18361 ttattgcgga atacatcgcc aatagtgcga acgttgtcgc cctgtacgac ttcatcagcc
    18421 catgctccgg cctgatacgg tgcatcttca tcgaatggcg attcactgcc cttgaacatc
    18481 gtggcggtga ttttcttgcc ggagaacgct tccgttgtct gtctgcgtag cccggcacca
    18541 acaccatcac catcccacag gtaatggtca gcgccgtctt caatcgccag cgaagtagcc
    18601 cagtcagcac cctcgttgat gtccatcagc agaccttcgg caatgcgctt aactaccgaa
    18661 ccgtgacgcg atgcataacc tttagcatct ggccctgtat ctgatgggtc atgcgcagag
    18721 acaacagcgc ctttcgcttt ccatccgagt ttcttgtgcg catcggttgc ggcttcaagc
    18781 cattcacgtt tgatgattgc catatcactt gcgctcactg gctcaccaag ccagatgtga
    18841 cgatacaggg tcggatttct gcgtttacac tcttccatct ccagacggag aacttcagga
    18901 aagtgcgggt tgtcggtgta gttcaccgtc agcaggcaaa tatcatctgg aggatttacg
    18961 acgaatcgct gataggtatc gtcgaggatg tttttcgggt taaagctcac ccatatttcg
    19021 gaaaatggct tgcggatggt tggtatcagg atatcccatg attccttcgt taccgcttcc
    19081 gcttcctcca cccagcagat atcaatgcct tcgagcgatt taatcttcgt cgggttgttt
    19141 ttgatgccgt agaacatgaa ctcagcattc gttccgagat gacgaatcat tgaacgctga
    19201 atttcaaact cagccgaata cccttcccgc tctatggtgt cttcaagcaa ccgaattacc
    19261 gaatcgctga tactgttttg cagttcacga gcgcagagaa tacgcactgg ctgccgacgc
    19321 gccgcttcaa caagcagtct cgcaattgcc catgacttac cgctacctcg accgcctttg
    19381 gcgactttgt agcgatgcgc ctcaataaac ggttcaaaga taggattaat cgtggtcatt
    19441 ttccgaatag agtgctcatc ggtgatgttt caatctgaat tgcgccgccg tctttgcctg
    19501 ttagctcgtg atcaaccttg tcgcgccatt tatccttctg tcggttctta agccagaaga
    19561 tggcagctgt tgtatcaggc gggtaatact tctcaagcgg agtttcgaca attctgtttt
    19621 caataacacg aatatcgatg tctggagcca cgaagcccat agcgcgttga taaagacgat
    19681 cactaacttc tgcatcagcg acggccttac ccttttttat ggactccgaa aactcaggat
    19741 aatcaagctt ccacttgtta atagttgact cactgacttc gaagaaatca gcaagctctg
    19801 catcggtgta gcccagcaag cacagtttgc gtgcctgttc ggcatacgcc tcttgatact
    19861 ttgttgggcg cgccatgttt atgctccggt ggtgaacagg tctaacgctt ccttcgattt
    19921 acgcaccgct tcgatagttc gggtcgtgat atctgaatta gcgccacctg actggaagtg
    19981 aattttgaat agctcaagct tcagttcgtc agtgccaatg aattgaaatg cttcttctgc
    20041 tgctgcgttc tggttcatga ccagtttgta aatctctaac tggaatttct gttcttcagt
    20101 catgggaata atctctgcca ttgttggctc cgtttatccg ttaaaaggga tatcagttaa
    20161 gttatcccgt gcagggtata agtcattgtc gagaccactt attgaatggc ctctgcaata
    20221 accgatgtct ttccatcagt ccgccaccac aaagaatctt ttttgccata aggcaggagg
    20281 ttcatctttc agtggctgcc agtgttattt ccccacttac tggcttgggt tgtttcgctg
    20341 tactgccgta actggttacc cagaataaat tccggtttca ttatcaagcc cacccgtaga
    20401 tgggctttgt aatggatagc tgttgctcag ttctcgtaat gctttgattt ttccgataac
    20461 gcagttttgc gtttgccatc agcacgcgat atcgagagtc aactgcagtt gctcgcgcca
    20521 gtactcaaca tttgcttcaa taaccggctt atcccatcgc cagcgagcca tctctcttgc
    20581 cccattgctg gcttttgatt tccggtcatc gcgaatgcga catgcttgct catatttctg
    20641 ctgctcagtc agttcaccac gaagcagact atcaatgtgc aggtcgcacc atacggagaa
    20701 tttcggatcg caccatcttg caaaggcaac tgataacttt gggtgcagcc atgttccgcc
    20761 tcccctgtcc tttcgtgcct tgcttgtttt tacatacccg gaatcacggg tatgtagaat
    20821 tttcgatggt tcacctgaat aaacctcatc cagagctcta acgtattcga gagtttcagc
    20881 gttggacaac cagtgatcca gacgcttccc gaaacgtttt gcaatatcag tggcattaat
    20941 ccagccatcc gtattgaagc ggataggttc gcctttgtaa ttcagtggaa cgatattcat
    21001 agcgtcttac cttttagaaa gatgagcctg ttcgcacaga aaagccgccc cgagatggtc
    21061 gccaccatat acggcaattc tcaggctcag ctttctgaaa gactcgggat tgttacgcgc
    21121 tgcgatgcgc ggtttactgc ggacataaaa aagccccgca tcgcgaggct cattaaatgg
    21181 actttgtgat ttgcaaaaaa attatttcag gcactgagtc ctgatgtact cctgcaggta
    21241 gttaacctgc gcggttatcc tgtcgattcc acttcggaga cggtaataat tgagttcagc
    21301 atctgctgta agtcctgggc tttctccatc gcccatgccg ctggctccgg tcgttgactt
    21361 tgcacaggtg gcggcgactt gcaggcgcct acgcccagca gaaacatcag cacggaggct
    21421 ttcgatagtc gcgttagcat cagcaagctc ctttgtgtat ctggcgtcga gttctgctac
    21481 atcacgttga cgcttctgca tatcagagat cgtcgccata gccgaatcta atgccatagc
    21541 gttttcgtcg cgctgttttt tgtattcaat ggctttattg tggtaatgat tagctgacca
    21601 gacgagagaa ccgaataaag tcaggaggaa agcagaaata accagcttat agcgaagttt
    21661 cattaaccaa cccgccagct tctttgaatt tggcaatcag actatcgatc ttgtgttcat
    21721 actgaccgta gccagcaccg ggcaatgaag cccaaatatt gctgcaacgg tcgattgcct
    21781 gacgaatatc accgcggtca atcatcggta aagcgccacg ctctttaatc tgttgcaatg
    21841 ccacagcgtc ctggcttttc ggagagaagt ctttcaggcc aagctgctta cgataggcat
    21901 cccaccaacg ggaaagaagc tggtaacgtc cggctgctgt tgattttagt ttggggttta
    21961 gcgtgacaag tttgcgaggg tgatcggagt aatcagtgaa tagctctcct cctacaatga
    22021 cgtcataacc atgatttctg gttttctgac gtccgttatc agttccctct gaccatgcca
    22081 gcatatcgag gaacgcctta cgttgattat tgatttccac catcttctac tccggctttt
    22141 ttagcagcga agcgtttgat aagcgaacca atcgagtcag taccgatgta gccgatgaac
    22201 acgctcgtta tataagcgag attgctactt agtccggcga ggtcgagaag atcacgaatg
    22261 aaccaggcga taatggcgca catcgttgcg tcgattactg tttttgtaaa cgcaccgcca
    22321 ttatatctgc cgcgaaggta cgccattgca aacgcaagga ttgccccgat gccttgttcc
    22381 tttgccgcga gaatggcggc taacaggtca tgtttttctg gcatcttcat gtcttagccc
    22441 caataagggg atttgctcta tttaattagg aataaggtcg attactgata gaacaaatcc
    22501 aggctactgt gtttagtaat cagatttgtt cgtgaccgat atgcacgggc aaaacggcag
    22561 gaggttgtta gcgcgacctc ctgccacccg ctttcacgaa gatcatgtgt agaaggccgc
    22621 agcgtaacta tcactgatga attcaggata gccagtggct acggctcagt ttaggttgtg
    22681 ctgttgctgg gcggcgatga cgcctgtacg catttggtga tccggttctg cttccggcat
    22741 tcgcttaatt cagcacaacg ataagagcac tcggtgcatt taagccaagc cccataaggg
    22801 agaatgctct tacctgttgc acagatataa aaaatcccga aaccgttatg caggctctaa
    22861 ctattacctg cgaactgttt cgggattgca ttttgcagac ctctcagcct gcgatggttg
    22921 gagttccaga cgatacgtcg aactgaccaa ctaggcggaa tcggtagtaa gcgccgcctc
    22981 ttttcatctc actaccacaa cgagcgaatt aacccatcgt tgagtcaaat ttacccaatt
    23041 ttattcaata agtcaatatc atgccgttaa tatgttgcca tccgtggcaa tcatgctgct
    23101 aacgtgtgac cgcattcaaa atgttgtctg cgattgactc ttctttgtgg cattgcacca
    23161 ccagagcgtc atacagcggc ttaacagtgc gtgaccaggt gggttgagta aggtttggga
    23221 ttagcatcgt tacagcgcga tatgcggcgc ttgctggcat ccttgaatag ccgacgcctt
    23281 tgcatcttcc gcattctttc tcaacaactc tcccccactg ctctgttttt gctatatcaa
    23341 ccgcacggcc tgtaccgtga caatctctgc atcttgcgcc cggcgtcgcg gcactacggc
    23401 aataatccgc ataagcgaat gttgcaagca cttgcagtac ctttgcctta gtatttcctt
    23461 caagctttgc cacaccacgg tatttccccg ataccttgtg tgcaaattgc atcagatagt
    23521 tgatagcctt ttgtttgtcg ttctggctga gttcgtgctt accgcagaat gcagccattc
    23581 cgaatccggc ttgtgattgc gccatcccca tagcagccat cacatcagta ccagaaagag
    23641 agtcagaagc cgtggcccgt ggtgagtcgc tcatcatcgg gctttttggc gaatgaaatt
    23701 tagctacgct ttcgagtctc atggccttcc ccttttgccc tgtttgacca tcaggacgcc
    23761 gttaactatt acgtgacgct cgcctttgtt gtctcggttg tacttgagca ctgttcctct
    23821 tgcgcaggaa agcatccttg ccacttcggt ctgattgcct cgtgtctgga taagaagctc
    23881 tggtatcgtt tgaattgtgg cgttcatacg ttctccagtt cggtgatttt tattccaagc
    23941 cttcctcctg gtactttcac accacgaatt acgcgaatgt catcgaattg ctcgtcgtct
    24001 tccgcaaatc cggcgtggat aagggagtcg agtaaaccct tcaggatgtt atcgaggtcg
    24061 cggcggcggg agtctggaac gtctgcgatg actttgatgc ggagtcgtga tttggtgaaa
    24121 atgtctaact tgagttggcg gatgatttgc tgaacgtctt ttcggtattt ctggccttta
    24181 tcgctgatgt agtattggct tccccgtctt cgccagtagg tgttcaccga cggcgggtat
    24241 gggagcacaa actgatattc gttcatggct taatcttccc ctccttcagc agtatcgcct
    24301 gcgtcctgat cacgccttcg aggtggtaaa gtctggcgtc tttgttgtcg agaatccggg
    24361 tgcgtcggtc gatctccgcg tggcagtcac tacaagccca tgcgccgatc aggtcgtcag
    24421 gcttcatccc cgttccacaa attccagtca tccggtaatg tgccagaact gtagtttcag
    24481 ggttgccatt gcatacgccg taaatacgta cctggcattc tctgccgcgc gcttctttgc
    24541 gtaggttaac cattaagcag cctctcctgt tactttcagc attccgttat cgagcagctt
    24601 tctggtcagc cactgttggc cacgtccggt gatttttgta gtgaacgata tctgtattcc
    24661 gtgatttgtg ttgaccgctg tttctttcac tgtgaaatag ccgcgatcca tatattcctg
    24721 cattggcaca ttgcgccggg aacctgaagc aataaggatt ttgtgatcgc gcatccacgc
    24781 aaacagtttg tttggaccaa ttccaacaac ctttgcaaag tttccaatca aaattccgct
    24841 ggcctcgcca acgcgatcgg caaactcaac tttaggtgcg gcaattgcga gctggttttc
    24901 cagttgcatt ttctgctcag caagatcagc agcaaggcgc aacgcttctg gtagcgtttt
    24961 ggggatatta accgcagttt cttcaagctc tcgccaacgg tcaacaagac gagcggtgaa
    25021 ttccggcgac aactgggcta caacgacaat actgtctcgc tttccttgtt cgccttcgaa
    25081 tacatacaca caaaaacttt gatttaagcc taacccattg attcttccac aatcctcaat
    25141 ttgaggaagc cggataacac catttttagc cagcgtttcg atggtacgtt tcacattgtc
    25201 atgacgctta ccaaccaact cagcgatttc aatgcttgtc attttgatgg cattgccatt
    25261 tattaactca ttcatcgtct tcttcctcgt acattgagct attcggatcg ctcatcagtt
    25321 ctgcgcagca gtgctcacac acgtgaactt ccagcacatg cagcttctga ccgcagttag
    25381 cgcacgttaa agctcgctcg acgctttctt tctggtattg aagggattgg gatgggctaa
    25441 gcattattgg cgtcctgcat catgagaaag acaatcatgg cggcgcggag tgggttttca
    25501 tcttgagtca tatgatatgg ggtactatca ctgccaactt ttctatgcgc tgccttccat
    25561 aatccatttt ctggcgctgg aataatgcca attctgttct ctacgataat cggctctgcg
    25621 tctgatgggc ttttacagta atcaaccgtt tttattgcat aaccagtttc gtcatcccac
    25681 tcaacaccaa cgattgatgt tcccaacttt gcgatttcgc aatcttcggg agtaaatcca
    25741 cagcaaattg ccactcgctt gttaatttca aaatcactta actgtgaata atccattgtc
    25801 atttcctcgc acgatgtctt agccaccgga tatcccacag gtgagccgtg tagttgaagg
    25861 tttttacgtc agattctttt gggattggct tgcgtttatt tctggagcgt ttcgttggaa
    25921 ggtatttgca gttttcgcag attatgtcgg tgatacttcg tcgctgtctc gccacacgtc
    25981 ctccttttcc tgcggtagtg gtaacacccc tgttggtgtt ctttcacacc ggagacacca
    26041 tcgattccag taaggttgat ttggtcggaa gcggttatct tctttgcatt caccgcaccg
    26101 ataacatcgc atcatgctgc ccgatctccc catcgcgctt tccattcgag agccagtcgc
    26161 gcttcgtctg accacttaac gccacgctct gtaccgaatg cctgtataag ctctaatagc
    26221 tccgcaaatt cgcctacacg catcctgctg gttgactggc ctattaccac aaagccattc
    26281 ccggcaaggt taggaacaac atcctgctgc tttaatgctg cggtaaacac acacttccag
    26341 ctttctgcat ccagccagcg accatgccat tcaacctgac gagagacgtc acctaagcag
    26401 gcccatagct tcctgttttg gtctaagctg cggttgcgtt cctgaatggt tactacgatt
    26461 ggtttggttg ggtctggaag gatttgctgg atagcttgaa tggcgttctg ctgatgaatg
    26521 gggcttctta gttcaaacgt tagtttcctc atggctcacc ttctgcacgc atggattaat
    26581 taacgccaaa atagcttctg gtttctcaat aacatcgaac cgctcaccac ttgccattct
    26641 tacaatcgta aatccggcat caaatagggt ttcgatgttg tcagagttaa cgtacaaggg
    26701 ctcatatgca cttctggtgc tttctatcac gcctgtgctt ggtggctgat agatgctgca
    26761 ttgcatggtt aattttataa atggcatata ctcactcctt cactttgact ccagcagcgc
    26821 gaatccgtcc ttcgcattca ctaattgcgt tgttataacc aaatgtaacc ccatcctcga
    26881 aatcggtaga aaatgcctca cgctctcttt ctccaggaca ctcaatctcg atagctgctc
    26941 gcgatgcctg ccacgcttgc caatacatct caaccatatt ggcgtatatt ttatttttag
    27001 gatcacatcc ggtgtaattt tcaaaccatt cttcaaactg ctttcttgat tcgtccatcg
    27061 atacttaccc tcagttcaac tcacaaaacg ccacgccatt tttgctacag cgacaggcat
    27121 aacaccgata atcacccaca ggagaatgct accgaacagc acatccacca ggtctttacc
    27181 ttcgcctacc aaccggacaa aacagccagc aaccacaatg aacgtcgcca ccatccacag
    27241 agcaccgaga agcctcaatg cagagaaaat caactcaacc acgatttact ctcccccaaa
    27301 taaaaaggcc tgcgattacc agcaggcctg ttattaactc agtgatgtag atggtcattt
    27361 aatactccgt cacgttttcc tgtctccacg cctcgtcata ttccgatttc ggcatattgg
    27421 cgatgtagct atatggcgat cctgattcaa gttgcaggaa ctggtgcgat tgctcgtcaa
    27481 ggaacaacgg gacaccacct tcccaacctt cgccgttacg ttgtttttca agcatcaaaa
    27541 cagatgccgg agatgccagt agctgttcgt ccttctctga catcttttca ccactctgaa
    27601 ctctctgtaa cgctctctcg cgagccttgt tacgccagat gatgaaaagg ttgtctgtca
    27661 ggtctgttat cgctccagag ccttttacgt ccattttccc ggttggtttt tcttcgctat
    27721 ctccttttct ggagtgagta acgagaatga cgtgggagtt tgttttgttt ttgaagtcac
    27781 aaatcgagtc aacaaatgct ttctgcccgt tatagtcatc gtcacctatg ccgcatttca
    27841 tcaggctgtc gatgatgaat aactggatgc cgtatcggcg gcgagcgtag tcgaatattt
    27901 cgatcagcct gtcggctttc gccgttccgg tcaggccaaa cacccaaagt ctttcgtcat
    27961 aaaatttaaa tgcagagtca atttccagca ctggcggcat cttgcagcac gtcgcctgac
    28021 gggtaaggcg cttaaggaga ataccaggct tcagctcaag tgacgcgatg cacgtcttca
    28081 caccctgacg cattgcctca agtgccatat gcccgacaac ctccgttttt ccgtgaccgt
//
LOCUS       DADLTY010000037        27944 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.037, whole genome shotgun sequence.
ACCESSION   DADLTY010000037 DADLTY010000000
VERSION     DADLTY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27944
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.037"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..1555)
                     /gene="gsiA"
                     /locus_tag="JN334_001364"
     CDS             complement(<1..1555)
                     /gene="gsiA"
                     /locus_tag="JN334_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HAY7437560.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYL"
     gene            complement(1542..2507)
                     /gene="iaaA"
                     /locus_tag="JN334_001365"
     CDS             complement(1542..2507)
                     /gene="iaaA"
                     /locus_tag="JN334_001365"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY7437561.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            2711..3946
                     /gene="moeA"
                     /locus_tag="JN334_001366"
     CDS             2711..3946
                     /gene="moeA"
                     /locus_tag="JN334_001366"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAY7437562.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            3946..4695
                     /gene="moeB"
                     /locus_tag="JN334_001367"
     CDS             3946..4695
                     /gene="moeB"
                     /locus_tag="JN334_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HAY7437563.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCIEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(4874..5536)
                     /gene="fsa"
                     /locus_tag="JN334_001368"
     CDS             complement(4874..5536)
                     /gene="fsa"
                     /locus_tag="JN334_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY7437564.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            5667..6566
                     /locus_tag="JN334_001369"
     CDS             5667..6566
                     /locus_tag="JN334_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAY7437565.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            6572..9004
                     /locus_tag="JN334_001370"
     CDS             6572..9004
                     /locus_tag="JN334_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAY7437566.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            9150..9965
                     /gene="ybiV"
                     /locus_tag="JN334_001371"
     CDS             9150..9965
                     /gene="ybiV"
                     /locus_tag="JN334_001371"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAY7437567.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            10117..11382
                     /locus_tag="JN334_001372"
     CDS             10117..11382
                     /locus_tag="JN334_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HAY7437568.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(11623..13215)
                     /locus_tag="JN334_001373"
     CDS             complement(11623..13215)
                     /locus_tag="JN334_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HAY7437569.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            13434..14354
                     /gene="ldtB"
                     /locus_tag="JN334_001374"
     CDS             13434..14354
                     /gene="ldtB"
                     /locus_tag="JN334_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7437570.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(14412..15530)
                     /locus_tag="JN334_001375"
     CDS             complement(14412..15530)
                     /locus_tag="JN334_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAY7437571.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(15527..15994)
                     /gene="mntR"
                     /locus_tag="JN334_001376"
     CDS             complement(15527..15994)
                     /gene="mntR"
                     /locus_tag="JN334_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HAY7437572.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            16180..16308
                     /gene="mntS"
                     /locus_tag="JN334_001377"
     CDS             16180..16308
                     /gene="mntS"
                     /locus_tag="JN334_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HAY7437573.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            16580..18163
                     /locus_tag="JN334_001378"
     CDS             16580..18163
                     /locus_tag="JN334_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY7437574.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(18211..18726)
                     /gene="ompX"
                     /locus_tag="JN334_001379"
     CDS             complement(18211..18726)
                     /gene="ompX"
                     /locus_tag="JN334_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HAY7437575.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(18779..18844)
                     /locus_tag="JN334_001380"
     CDS             complement(18779..18844)
                     /locus_tag="JN334_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437576.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            19079..19966
                     /gene="rhtA"
                     /locus_tag="JN334_001381"
     CDS             19079..19966
                     /gene="rhtA"
                     /locus_tag="JN334_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HAY7437577.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFFWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            20265..20768
                     /gene="dps"
                     /locus_tag="JN334_001382"
                     /gene_synonym="pexB"
     CDS             20265..20768
                     /gene="dps"
                     /locus_tag="JN334_001382"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HAY7437578.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            21172..21918
                     /gene="glnH"
                     /locus_tag="JN334_001383"
     CDS             21172..21918
                     /gene="glnH"
                     /locus_tag="JN334_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HAY7437579.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            22057..22716
                     /gene="glnP"
                     /locus_tag="JN334_001384"
     CDS             22057..22716
                     /gene="glnP"
                     /locus_tag="JN334_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HAY7437580.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            22713..23435
                     /gene="glnQ"
                     /locus_tag="JN334_001385"
     CDS             22713..23435
                     /gene="glnQ"
                     /locus_tag="JN334_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HAY7437581.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            23857..25920
                     /gene="ybiO"
                     /locus_tag="JN334_001386"
     CDS             23857..25920
                     /gene="ybiO"
                     /locus_tag="JN334_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HAY7437582.1"
                     /translation="MLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQK
                     VTEVSRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIR
                     LCALPLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNA
                     GSRTIAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWGRRLSWLS
                     SLIGYGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHMLNFAE
                     HSLAFFSLFIRAFALVWHWLASAYFIMLFFFSLFDPGNSLKFMMGATVRSLAIIGIAA
                     FVSGMFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAW
                     GLFDFWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSA
                     RTRTLLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDI
                     ITGVFIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANF
                     VRGIGSVVANYDVDRHEDADKANQALKDAVTELMENEEIRGLIIGEPNFAGIVGLSNT
                     AFTLRVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPP
                     GEPTL"
     gene            complement(25917..26843)
                     /gene="rlmF"
                     /locus_tag="JN334_001387"
     CDS             complement(25917..26843)
                     /gene="rlmF"
                     /locus_tag="JN334_001387"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HAY7437583.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            27119..27379
                     /locus_tag="JN334_001388"
     CDS             27119..27379
                     /locus_tag="JN334_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7437584.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            27644..>27944
                     /locus_tag="JN334_001389"
     CDS             27644..>27944
                     /locus_tag="JN334_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HAY7437585.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         6757 a         7435 c         7024 g         6728 t
ORIGIN      
        1 acagatacgc aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc
       61 gaatagaaac atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg
      121 ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac
      181 gccaggcatg ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg
      241 cgtctttacc tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg
      301 tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca
      361 atgcctgaag tttgccaggt gacaaggtat cgattcgctg accgttaaag ataatttcgc
      421 cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag
      481 actcgcccac cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg
      541 catgcacttc ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa
      601 gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt
      661 cctgtttcgc tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt
      721 tcatcgcacc aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg
      781 catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta
      841 cccgatcggc aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg
      901 acatctcttt ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca
      961 gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa
     1021 tcatcactcg ctggcgcatc ccgccagaga gttgatgcga ataacgtgaa agaatggttt
     1081 gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac
     1141 gactggcgtt ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaatactg
     1201 ggttcagcga tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatggc
     1261 gcatttgtgc agcgctctgc tcgctaagtt caatcacatc acgactgcgc cgccgcaaca
     1321 gcattttatc gcactgtact aaaccgcccg cctgttccaa cagacgcatc aatgccagcg
     1381 cagtcactga cttaccggag ccggattcgc ctacaattgc cagcgtctca ccgcgttgca
     1441 gactaaaaga gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat
     1501 taagattttc aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg
     1561 tccccttttt cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga
     1621 tacattcctt cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag
     1681 ccaccgctac cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg
     1741 agacttaatc cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg
     1801 aagacttcgc ctgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg
     1861 gcacccacta agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg
     1921 gacgtggctg ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt
     1981 ttctgttttt catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc
     2041 gctgccagta gttgttcata acgcaaaggc gtggagaaaa tctccggtga gacgcgctcc
     2101 atgccatgag caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt
     2161 tgctccatca ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc
     2221 gcaccggctt tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca
     2281 cgcgtaaaga cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc
     2341 gcttccgtca ccacatccag cgcactttcg ccggcttcca gcattttctg cccggtttca
     2401 acaatggcag acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg
     2461 ctaattgcac ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat
     2521 taagggtggc tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat
     2581 ttttgaatat agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg
     2641 tggcatacag tctatggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag
     2701 gagtgttttc atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat
     2761 gctttctcgc gttaccccac tgaccaccca ggaaacgctg ccactggttc agtgttttgg
     2821 tcgtattctg gcgagcgatg tcgtttcgcc actggatgtc ccggggtttg ataactccgc
     2881 tatggacggc tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc
     2941 cggtaaatcc tttgccggtc agccatacca tggtgaatgg cctgcgggta cctgcattcg
     3001 tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac
     3061 tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg
     3121 ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac
     3181 taccgcagag ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa
     3241 agtgcgtgta gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg
     3301 cgacggccaa atctacgaca ccaaccgtct cgccgtacac ctgatgttag aacagctggg
     3361 ttgcgaggta atcaacttag ggattatccg cgacgatcca catgccctgc gcgccgcatt
     3421 tattgaagcc gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga
     3481 agcggattac accaaaatga ttcttgaaga gctgggggag atcgccttct ggaagctggc
     3541 gattaaacca ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct
     3601 gccgggcaac ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc
     3661 aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc
     3721 ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc
     3781 cgacggtgaa cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt
     3841 tagtctcggc aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg acgtgcgcga
     3901 atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc
     3961 gatcaggaga tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt
     4021 caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca
     4081 gcttcgcagt atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg
     4141 gtttcgctct cgaatctgca acgccagaca ctgcacagtg atgccacggt cgggcaaccg
     4201 aaggtggaat ccgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca
     4261 gtcaatgcac tgctagatga cgcagaactt gcagcattga ttgctgaaca cgatctggtg
     4321 ctcgactgta cggataacgt tgcggtacgt aatcaactga acgcaggctg ttttgccgcg
     4381 aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact
     4441 tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta
     4501 acctgcatag aagcaggcgt gatggcaccg ttgataggcg taattggttc gttgcaggca
     4561 atggaagcga tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg
     4621 tacgatgcga tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag
     4681 gtgtgcgggc agtaattgcc acctccaagc ctgataagac gcgacaagcg tcgcatcagg
     4741 catttgctcc agacgccaga tgcggcgtga acgccttatc tggcctacgg ttcgggcaca
     4801 gatttccaag cgcggcaggc atcggtgcac tccgccacgt catcagcaat ttatgaggat
     4861 gcagaacgtc cggttaaatc gacgttctgc caaacgctcc ctgccagtcc tgctcaaact
     4921 tcgtcacagc ggcttccacc gccggatagc taatcatctg ttgtgccaca tccagtggca
     4981 gagtaattga ttcacatccg gccagtaagc agtccagcgc ctgacgcggg gttttgaaac
     5041 tcgctgccag cactttcgcc tgcggcgcat gcattttcaa taactggtgt aagtcggtca
     5101 cagtctgaat gccgctaccg ccctgagcat caatacgatt aacgtaaggc gcaacatatt
     5161 ccgcacctgc cagcgccgac agcagccctt gtgccgcgcc atataccgcg gttcccagcg
     5221 tcggaatccc ttccgctttt aacatcttaa tcgctgccag cccctcggct gtcaccggaa
     5281 ctttcaccac gatatccgca ataatagaac gcagcttaag cgcgtcattc accatccctt
     5341 cagcagtggt agccattacc tgggcaaaca gacgcccctg accgcccatc gcttcatgaa
     5401 gttgcggaag cacaacatcc agcggttttt tacccgcggc gataatgctt gggttagtgg
     5461 tcacacccgc cagcggaaaa atacgtgaca gcgccttcac cgcaacaacg tctgaagtat
     5521 ccagatacag ttccataacc atcctcaaaa tgtacttttt gtctgaatgt agactatcac
     5581 gggaaaactc tctgaacagt tgacgcacat caatctaact ttcattcgaa agtattttaa
     5641 tgtttgaacg aaacagagag gcaactatga ttttcaatat tcagcgctac tcgacccatg
     5701 atggccccgg tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt
     5761 gtcagaaccc ggaaagccgc gcccgtacgc aggatctgct gtatgacgca cgactgtgtc
     5821 tggaaggctg cgagctgtgc gctaaggccg cgccggaagt gattgagcgc gcgctgaatg
     5881 gtttgcttat tcatcgggaa aagttaaccc cggagcatct gacggcgtta accgactgct
     5941 gtccgacaca ggcgttaacc gtgtgtggtg aagtgaaaag cgttgaggag atcatggcga
     6001 ccgttctgcg cgataaaccg ttttacgatc gcagcggcgg cggcttaacg ctttcgggtg
     6061 gtgaaccctt catgcagccg gaaatggcga cggcgctgct gcaagcctgc cacgaggcag
     6121 gcattcatac tgcggtagaa acctgtctgc atgtgccgtg gaaatatatc gccccttctc
     6181 tgccctatat cgatctgttt cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac
     6241 agtggaccga cggtaacgcc gccagggtgc tggataacct gaaaaaactc gccgcagcgg
     6301 gcaaaaaaat cattatccgc gtgccgctga ttcagggctt taatgccgac gaaacctctg
     6361 taaaagccat taccgatttt gccgccgacg agctgcacgt tggcgaaatt cattttctgc
     6421 cctaccacac gctgggcatc aacaaatatc acttacttaa tctgccctat gacgccccgg
     6481 aaaaaccgct tgatgcgcca gagctgctcg attttgccca gcagtatgcc tgccagaaag
     6541 ggttaaccgc gaccttacga ggataacaac catgaccaca ctgaaactgg acacgctcag
     6601 cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt gtgaagccgc cagtctgtac
     6661 cgagcgcgcg cagcactata ccgagatgta tcaacaacat ctcgataagc cgatcccggt
     6721 acgtcgcgcg ctggcactgg cgcatcacct ggcgaatcgc accatctgga tcaaacacga
     6781 tgagttgatc attggcaacc aggcaagcga agttcgcgcc gcgccgatct tcccggaata
     6841 tactgtctcg tggatcgaaa aagagattga tgatctggca gatcgtcccg gtgctggctt
     6901 tgcggtgagc gaagagaaca aacgtgttct gcatgaagtg tgcccgtggt ggcgcggtca
     6961 gacggtacag gatcgctgct atggcatgtt taccgatgag caaaaaggtc tgctggcgac
     7021 cggcatcatt aaagcggaag gcaatatgac ctccggcgat gcgcacctgg cggtcaattt
     7081 cccgctgctg ctggaaaaag ggcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc
     7141 gcgcatcaat ctgacggtgc tggaagattt gcacggcgaa cagttcctga aagcgattga
     7201 tatcatgctg gtggcagtca gtgaacacat tgaacgtttc gctgccctgg cgcgtgcaat
     7261 ggccgcgacc gaaacccgcg aaagccgtcg cgatgaactg ctggcgatgg cagaaaactg
     7321 cgatcttatc gcccaccagc cgccgcagac tttctggcag gcgctgcaac tgtgttactt
     7381 catccagttg attttgcaga ttgaatctaa cggtcactcg gtgtcgtttg gtcgtatgga
     7441 tcagtatctc tacccgtact atcgccgcga cgttgaactg aaccagacgc tggatcgcga
     7501 acacgccatc gaattgctgc atagctgctg gttgaaattg ctggaagtga acaaaatccg
     7561 ctccggctcc cactcaaaag cctctgcggg aagtccgctg tatcagaacg tcaccattgg
     7621 cggacaaaat ctggttgatg gtcaaccaat ggacgcggtg aatccactct cttacgcgat
     7681 cctcgaatcc tgcggtcgcc tgcgttccac tcagcctaac ctcagcgtgc gttaccatgc
     7741 aggaatgagt aacgatttcc tcgacgcctg cgtacatgtg atccgctgcg gcttcgggat
     7801 gccggcattc aacaacgacg aaatcgtgat cccggaattt attaaactcg gtattgaacc
     7861 gcaggacgct tatgactacg cagcgattgg ttgtatagaa accgccgtcg gtggcaaatg
     7921 gggctatcgc tgtaccggca tgagctttat caacttcgcc cgcgtgatgc tggcggcgct
     7981 ggaaggcggg cgtgatgcca ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc
     8041 ggcaggtaac ttcaacaact tcgatgaagt gatggacgcg tgggatacgc aaatccgtta
     8101 ctacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac accatgctgg aagagaacgt
     8161 gcacgatatt ctctgctcgg cgctggtgga tgactgtatt gagcgagcga aaagtatcaa
     8221 gcaaggcggc gcgaaatatg actgggtttc tggcctgcag gtcggtattg ccaacctcgg
     8281 caacagcctg gcggcagtga agaaactggt gtttgaacag ggcgcgattg gtcagcaaca
     8341 gctggctgcc gcactggcgg atgacttcga tggcctgact catgagcaac tgcgtcagcg
     8401 gctgattaac ggtgcgccga agtacggcaa cgacgatgat accgtcgata cgctgctggc
     8461 tcgcgcttat cagacctata tcgacgaact gaaacagtac cataacccac gctacggtcg
     8521 tggtccggtt ggcggcaact attacgcggg tacgtcgtct atctccgcta acgtaccgtt
     8581 tggcgcgcaa actatggcaa cgccggacgg gcgtaaagcc cacaccccgc tggcagaagg
     8641 cgctagtccg gcctctggta ctgaccatct cggccctact gcggtcattg gctcagtggg
     8701 taaactgcct acggcagcga ttctcggcgg cgtgttgctc aaccagaaac tgaatccggc
     8761 aacgctggag aacgaatctg acaagcagaa actgatgatc ctgctgcgta ccttctttga
     8821 agtgcataaa ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc
     8881 gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt gtcgcgggtt attccgcctt
     8941 cttcaccgcg ctctctccag acgctcagga cgatatcatc gcccgtactg aacatatgct
     9001 gtaatctctt cggcccggct gcggcgtcgc cgggctaaat cattcacatc atcaatttca
     9061 tccttacttt cattcgaaat ataatttgtg ctctgcgtca cattgttatt acactgttac
     9121 ggtttcattt tcgagccagg agcacaccta tgagcgtaaa agttatcgtc acagatatgg
     9181 acggtacttt tcttaacgac gccaaaacgt acaaccaacc acgttttatg gcgcaatatc
     9241 aggaactgaa aaagcgcggc attaagttcg ttgtcgccag cggtaatcag tattaccagc
     9301 ttatttcgtt ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac
     9361 tggtttacga acatggcaag caactgttcc acggcgaact gacccgacat gaatcgcgga
     9421 ttgttattgg cgagttgcta aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa
     9481 gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc actgatggca aaacactacc
     9541 atcgcctgaa acctgtaaaa gattatcagg agattgacga cgtactgttc aagttttcgc
     9601 tcaacctgcc ggatgaacaa atcccgttag tgatcgacaa actacacata gcgctggatg
     9661 gcattatgaa acccgtcacc agtggttttg gctttatcga cctgattatt cccggtctac
     9721 ataaagcaaa cggtatttcg cggttactga aacgctggga tctgtcaccg caaaatgtgg
     9781 tagcgattgg cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt tattcctttg
     9841 cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg ttacgctacc gatgataata
     9901 atcatgaagg cgcgctgaat gtgattcagg ctgtgctgga taacacatcc ccttttaaca
     9961 gctgacctct ctaccggagc catgcgctcc ggttttccct tctttttaca tcctgtacat
    10021 cacatcaaat cattaaactt gcattaactt atttataact tgtattgtca catgcaaatt
    10081 agcatagttt cgaatgaaag ttaatgcaag gtggttatgg cttctacttt taccagcgac
    10141 acattgcctg ccgatcacaa agcagctatc cgtcagatga agcacgcgct gcgggcgcag
    10201 cttggcgatg tccagcagat ctttaatcag ctaagcgatg acattgccac gcgagtggct
    10261 gaaatcaacg cactcaaagc acagggcgat gccgtctggc cggtgctgtc ttatgccgat
    10321 atcaaagcag gtcatgttac tgcagagcag cgcgaacaga ttaaacgtcg cggttgtgcg
    10381 gtgataaaag gccatttccc ccgcgaacaa gcgctaggct gggatcagtc gatgctggac
    10441 tatctgaacc gcaaccgctt tgacgaggtc tacaaaggcc ccggcgataa tttcttcggg
    10501 acgctcagcg cttcacgtcc cgagatttac cccatctact ggtcgcaggc gcaaatgcag
    10561 gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc tcaatcgtct gtggacattt
    10621 gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg tcatctaccc tgaccgtatc
    10681 cgccgccttc cgcccggaac gacctccaaa ggtcttggag cgcataccga ctccggggcg
    10741 ctggaacgct ggctgcttcc agcgtatcag cacgttttcg ccaacgtctt taatggcaat
    10801 ctggcgaaat acgatccctg gcatgcggca catcgtacgg aagttgaaga gtacacggtg
    10861 gacaacacca ccaaatgttc cgtgtttcgg acattccagg gctggactgc gctctcggat
    10921 atgctgcctg gtcaggggtt gctgcacgtc gtgcccattc ctgaagccat ggcgtacgta
    10981 ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac tgtgcggcgt agcgcccgga
    11041 agagtgttgc cggtatcaga gcaatggcat ccactgttaa ttgaggcgtt aaccagcatt
    11101 ccaaaactcg aggccggaga ctccgtctgg tggcactgcg acgtcatcca ttccgttgcc
    11161 cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca ttcctgcggc accgatgtgc
    11221 gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac tggaaaaagg cgcatcgccg
    11281 ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag gacgctttac gcttgccgac
    11341 ctcaacattc acggtaagcg agcgttgggc atggatgttt gagagttaat ggaagtgaat
    11401 tgatgtggga ttaagaaaat cgattcgacg ttactcagat tgctgacaag tgcgctttct
    11461 taatgcctga tgcggcgtta acgccttatc cggcctacaa agtcctgcag attcaatata
    11521 ttacaggtac tgcgtaggcc tgataagcgt agcgcatcag gctattttgc atttgtcatc
    11581 agccgtcgtc accccgacag tatcggggtg acggataatc acttactcga tccctttact
    11641 acgcaggtaa tcttcgtaat taccgctaaa gtcgatcacg cgttccgggg tgatttccag
    11701 aatgcgggtc gccagggagc ttacgaactc acggtcgtgt gaaacaaaga tcagcgtgcc
    11761 ctgatacagt tccagcgcca tgttcagtga ctcgatggat tccatatcca ggtggttggt
    11821 cggttcgtcc atgatcagaa tgttcggctt ctgcatcatt aacttaccaa acagcatccg
    11881 ccctttttcc ccaccggaaa gcactttagc tggctttttg atgtcgtcct ggctgaacag
    11941 caaacgaccg agaatgctgc gtaccgcctg ctcgtcatcg ccttcctgct tccactgact
    12001 catccattcg aacacggtca gatcattttc aaactcatat tcgtggtcct gggcgtagta
    12061 accaatgcgc gcgttttcag accattttac ggtgccgctg tccggttgca gatcgcccac
    12121 cagcgttttc agcagcgttg atttaccgac gccgttggta cccagtaccg caagtttttc
    12181 accgacctcc agcagcaggt tgagattttt aaacagtgga ccgttatcaa accctttggt
    12241 cagaccttcc acttccagcg cgttacggaa cagtttctta tcctgttcaa aacggataaa
    12301 cgggttctga cggctggagg ctttcacctc ttccagtttg attttatcaa tctggcgcgc
    12361 gcgcgaagtt gcctggcgag atttcgaggc gttggcgcta aagcggctaa cgaaagattg
    12421 caactcagca atctgcgctt tcttcttggc gttatcggcc agcagacgtt cacgcgcttg
    12481 ggtcgccgcc gtcatgtact catcgtagtt acccggataa acgcgcagct cgccgtaatc
    12541 cagatccgcc atgtgggtac agaccatgtt aaggaagtga cggtcgtgcg agatgatgat
    12601 catggtgctg tcacgctcgt tcagcacctg ttccagccag cgaatggtgt cgatgtcgag
    12661 gttgttggtc ggttcgtcga gcaggagaat atccggctca gcaaacagcg cctgcgccag
    12721 aagcacacgc agcttccagc caggagcaac ttcactcatc gggccgtagt gctgttccac
    12781 tggaattccc acgccaagca acagttcacc ggcgcgagct tccgcagagt aaccgtccat
    12841 ttcgccgtat ttcacttcca gatcggccac tttatagccg tcttcttcac tcatttccgg
    12901 caaagcatag atgcggtcgc gctcctgctt cacttcccac aactctttat gccccatgat
    12961 caccgtatcc agcacagtga actcttcaaa ggcaaactga tcctgacgca gcttaccaat
    13021 gcgctcgttg ggatcgaggg aaacgttacc cagcgtcggc tcaaggtcgc cgccgagaat
    13081 cttcataaag gtggatttac cactaccgtt cgcgccaatc aggccgtaac ggttgccgcc
    13141 gccgaatttg acggaaatgt tttcaaacaa cggcttactg ccgaactgca tggtgacgtt
    13201 actggaaact aacacaggcg tatcctgaaa agagatatga caaaccgcgc attatgccat
    13261 aagtctgaaa gaagatcctg ctcctcgctt aaggtctaca ctcaagccaa aggcaaaaaa
    13321 tgtgatttcg tacacatctg atttcactgt gagctggaat gaacttataa tgcgcttcca
    13381 atactctaat attctcaacc caatggcctg ccaggcacaa aatctcgctt aacatgaata
    13441 tgaaattgaa aacattattc gcagcggcct tcgctgttgt cggcttttgc agtaccgcct
    13501 ctgcggtaac ttatcctctg ccaaccgacg ggagtcgcct ggttggtcag aatcaggtga
    13561 tcaccattcc tgaaggtaac actcagccgc tggagtattt tgccgcggag taccagatgg
    13621 ggctttccaa tatgatggaa gcgaacccgg gtgtggatac cttcctgccg aaaggcggta
    13681 ctgtactgaa cattccgcag cagctgatcc tgccggatac cgttcatgaa ggcatcgtca
    13741 ttaacagtgc agagatgcgt ctgtattact atccgaaagg gaccaacacc gttatcgtgc
    13801 tgccgatcgg cattggtcag ttaggcaaag atacgcctat caactggacc accaaagttg
    13861 agcataagaa agcaggcccg acctggacgc cgaccgccaa aatgcacgca gagtaccgcg
    13921 ctgcgggcga accgctaccg gctgtcgttc cggcaggtcc ggataacccg atgggtctgt
    13981 atgcactcta catcggtcgc ctgtatgcta tccatggcac caacgccaac ttcggtatcg
    14041 gcctgcgtgt aagtcatggt tgtgtgcgtc tgcgtaacga agacatcaaa ttcctgttcg
    14101 agaaagtacc ggtcggtacc cgcgtacagt ttattgatga gccggtaaaa gcgaccaccg
    14161 agccagacgg cagccgttat attgaagtcc ataacccgct gtctaccacc gaagcccagt
    14221 ttgaaggtca ggaaatcgtg ccaattaccc tgacgaagag cgtgcagaca gtgaccggtc
    14281 agccagatgt tgaccaggtt gttcttgatg aagcgatcaa aaatcgttcc gggatgccgg
    14341 ttcgcctgaa ttaatcgtca aaacgtaaaa caaaggcgga ctcataatcc gcctttttta
    14401 tttgccagac cttaccaggc cgggatcata actaacaaaa cgtatcccac cagcgccgcc
    14461 cacaacagca tcggtattga atagagatgg aaacgccacc agatgcggcg atcgttcgcc
    14521 atacgtagcg caatcaaatt tgccagcgat cctggtaata aaccaaagcc ccccacgttt
    14581 accgcccatg ccagtaataa agacggaggt acataattga gcagcaaaat ggtactcggc
    14641 acgttactga tcacctgcga taaaccgact gccgttaacc ataacccggg ttcagataaa
    14701 tgactcacgt tacccaacac gccttgcaac gctggaagct gggtcagtaa atggacgtcg
    14761 ataaacatcg ccataaacac cagcagcaac gtccagtcca cactcaacac cacgcgacgc
    14821 gccagcagca caaagcccgc cgccacaatc accagtcccc acagctcttg tttgaactcc
    14881 agcgccgtca gaaagacgat atacagcccc agacaattcc acaccagccg cggtttccac
    14941 tccggtgttt gcacccccgt atggtattgc agcgcctttc cagggaaaca acaccagcac
    15001 aacagcagga gtgtcagcat cattgcgcca gccatcggtg ccatttgggc aataaatccg
    15061 gcaaacgaaa gaccagaacg tccccagata agaatatttt gcgggttgcc aattggcgtc
    15121 agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt aaccgggatc
    15181 tcacacagtc tttttagcgt gatagtcagc ggaacaacaa taaacagcgc gacatcgttg
    15241 gtcagaaagg tagaaagcag cgccgccgcc agcaccataa acatcgccag ccgacgctcc
    15301 gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa aataaccgct taactccaca
    15361 cctttggtca gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc gatagcagca
    15421 ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac aagaattaat
    15481 aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag gcaggctcat ttggcaccgt
    15541 gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc actaacatgg tgctccatgc
    15601 cttccgcgtc gcgacgggcg atttccggac tgacgcccaa caccaacaag aaattttcga
    15661 ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc tccttctgcc gttaaaaaca
    15721 cgccacgcca ggggatcatt tcaatcagcc ccatggttgc cagccgctta agcattttag
    15781 ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc cacctgacga gcttccccca
    15841 cttccctgat caagtcagaa atcagctcaa cgtaatcatc aataagctcg cgccgatgcg
    15901 cctctctgac ctggcggaac ccttcaacgt gctcttccac gttcactaac tgcgtcactt
    15961 tttttgctat tggcgtacct gcgcgacgac tcattgtgct tcctcattat ggtgacgcat
    16021 acatagcgtt ctcatttcgt gcgcacattg taaaccagag ttgcgaaggt acaaaaaatt
    16081 aacgttttag caatagctat ataatatagc ctgtgctata tctgtatgta atgcaatcat
    16141 ccctcaagga tcgacgggat tagcaagtca ggaggtctta tgaatgagtt caagaggtgt
    16201 atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa tgctgctctc tatgttgtgc
    16261 gatatggtca acaacaaacc gcagcaagat aaaccttcag ataaatagcg gcgtcgcggt
    16321 acgccgcttc actcctgctt ttatgcaggc ataacgcgtt ttggtctgaa aaaaaccact
    16381 ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc gtcagtagtt gacctgaacg
    16441 gcggctcgct ctatcttctt gcagccctgc gtatattgcg gctcgcggat gcggacccct
    16501 ttcccctctt cacgcactct tgcaggtatt gacccttgac gccagggtaa gcacatggcg
    16561 tttgttacga tagtggcata tgaatttaac cctcaaagaa tcgcttgtta cccgtagccg
    16621 ggtatttagc ccgtggactg cgttctactt tttacagtcg ctattaatta acctcggctt
    16681 aggttacccc ttcagtttgc tctacaccgc tgcgtttacg gctattttgc ttttgctatg
    16741 gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc agttcgctgg tggcggcttg
    16801 ttatttccct tttgctcagg cctacggcgc gcctaatttc aatacattgc tggcattgca
    16861 ctccaccaat atggaagagt cgaccgaaat cctgacgatt tttccgtggt acagctacct
    16921 ggtcggctta tttatttttg cgctcggcgt aatagcaatc aggcgaaaaa aagagaatga
    16981 aaaagcgcgc tggaatacct tcgacagcct gtgtctggta ttcagtgtgg cgacattttt
    17041 tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt aaactgaaag atactggcta
    17101 tccggtattt cgttttgcta aggatgtcat cgtcaataat aacgaggtga ttgaagagca
    17161 agaacggatg gcaaaacttt ccggaatgaa agatacctgg acggtcactg ccgttaagcc
    17221 gaagtatcag acctatgtgg tagtgatcgg tgaaagcgcg cgtcgcgatg ccctcggtgc
    17281 ctttggcggt cactgggaca ataccccgtt tgccagcagc gttaacggtt tgatatttgc
    17341 tgactacatt gctgccagtg gctccacgca gaaatcgctt ggcttaacgc tcaatcgcgt
    17401 agtcgatggc aaaccacagt ttcaggataa ctttgtcacc ctggcaaatc gcgcgggctt
    17461 ccagacctgg tggttttcca accagggcca aatcggcgaa tacgatactg ctatcgccag
    17521 catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa ggtaattttg aagcagataa
    17581 aaacacgaaa gacgaagcgt tactggatat gaccgctcaa gtgctggcgc aagagcactc
    17641 gcaaccgcag ctgattgttc tgcatctgat gggctcgcat ccgcaggcct gcgacaggac
    17701 acaaggcaaa tacgaaacct ttgtgcaatc gaaagaaacg tcgtgctatc tctataccat
    17761 gacgcaaacg gacgatttac tgcgcaagct gtacgatcag ttacgcaaca gcggcagcag
    17821 cttctctctg gtttactttt ctgaccacgg tctggccttt aaagagcgcg gcaaagacgt
    17881 gcaatacctt gcccatgatg ataaatatca gcaaaatttc caggtgcctt ttatggtcat
    17941 ttccagcggc gataaagcgc atcgggtgat taaagcccgc cgctcagcca atgacttctt
    18001 aggctttttc tcgcagtgga cgggaattaa agcgaaggaa ataaatatca aatacccgtt
    18061 tatatctgag aagaaagccg ggccgatata catcaccaac ttccagttac agaaggtgga
    18121 ttacaaccat ctcggaaccg atattttcga cccgaaacct taaaaacaaa aatccgcccc
    18181 gagaggcgga tttttatatc accaaagtga ttagaagcgg taaccaacac cggcaatcca
    18241 ggtgcctacg tcaacgctac gaatacggct ctgctcgtaa gagaagtcca gagcaacgtt
    18301 ttccatcggg ttgaactgca gaccagcacc gtaggagaaa ccgtagtcgc tggtgtcgtg
    18361 tttgtaggtc gggtattcag tggtctggaa tttaccataa cccacaccca ctacaccgta
    18421 gatgcttgcc cagtcgttaa tgcggtaagc cggaccagca gtgatgccgt agtactggtt
    18481 tttgttgtag tcaccagagc ttgcagtacg gcttttctcg gtgtaagtga aagaaccgat
    18541 cacacccagc gggctgttgt cttcttcata gcggtatttc aggttgaaac cgcccatttt
    18601 gttcatttgg ccctgagcgt cgctctgtgc gtaaccgcca gttacggtag aagtcgcagc
    18661 tacggaagta cctgcggtga aagccagaac tgcggccagt gctgaaagac atgcaatttt
    18721 tttcataacc acctcaaatg tgattcaaat aagtcctaag ttttaaatat atcaaaaatt
    18781 aatgggaaac tcttcgcgat ttgtgatgtc taacgggcca tttcatgtaa cagaacgttt
    18841 ccatacaccg ctatccatct aaatttaaat cactttttca gagaactgcg taagtattac
    18901 gcatgttttc cctgtcattc atccagatta ttcctaatca ccagactaat gattccatca
    18961 atcctggcgc attttagtca aaacggggga aaattttttc aacaaatgct caaccagcat
    19021 tgggtatatc cagtacactc cacgctttac ttaagtctag atatttgtgg gagaaaggat
    19081 gcctggttca ttacgtaaaa tgccggtctg gttaccaata gtcatattgc tcgttgccat
    19141 ggcgtctatt cagggtggag cctcgttagc taagtcactt tttcctctgg tgagcgcacc
    19201 gggtgtcact gcgctgcgtc tggcattagg aacgctgatc ctcatcgcgt tctttaagcc
    19261 atggcgactg cgctttgcca aagagcaacg gttaccgctg ttgttttacg gcgtttcgct
    19321 gggtgggatg aattatcttt tttatctttc tattcagaca gtaccgctgg gtattgcggt
    19381 ggcgctggag ttcaccggac cactggcggt ggcgctgttc tcttctcgtc gcccggtaga
    19441 tttcttctgg gttgtgctgg cggttcttgg tctgtggttc ctgctaccgc tggggcaaga
    19501 cgtttcccat gtcgatttaa ccggctgtgc gctggcactg ggggccgggg cttgttgggc
    19561 tatttacatt ttaagtgggc aacgcgcagg agcggaacat ggccctgcga cggtagcaat
    19621 tggttcgttg attgcagcgt taattttcgt gccaattgga gcgcttcagg ctggtgaagc
    19681 actctggcac tggtcggtta ttccattggg tctggctgtc gttattctct cgaccgctct
    19741 gccttattcg ctggaaatga ttgccctcac ccgtttgcca acacggacat ttggtacgct
    19801 gatgagcatg gaaccggcgc tggctgccgt ttccgggatg attttcctcg gagaaacact
    19861 gacacccata cagctgctgg cgctcggcgc tatcatcgcc gcttcaatgg ggtctacgct
    19921 gacagtacgc aaagagagca aaataaaaga attagacatt aattaaattt acatttctgt
    19981 atggttatgc ataaccatgc agaatttctc gctacttttc ctctacaccg tatttatata
    20041 tcgaattatg caaaagcata tttattccga aaattcctgg cgagcagata aataagaatt
    20101 gttcttatca atatatctaa ctcattgaat ctttattagt tttgtttttc atgcttgtta
    20161 ccactattag tgtgatagga acagccagaa tagcggaaca catagccggt gctatactta
    20221 gtctcgttaa ttactgggac ataacatcaa gaggatatga aattatgagt accgctaaat
    20281 tagttaaatc aaaagcgacc aatctgcttt atacccgcaa cgatgtctcc gacagcgaga
    20341 aaaaagcaac agtagagttg ctgaatcgcc aggttatcca gtttattgat ctttctttga
    20401 ttaccaaaca agcgcactgg aacatgcgcg gcgctaactt cattgccgta catgaaatgc
    20461 tggatggctt ccgcaccgca ctgatcgatc atctggatac catggcagaa cgtgccgtgc
    20521 agctgggcgg tgtagctctg gggaccactc aagttatcaa cagcaaaacc ccgctgaaaa
    20581 gttacccgct ggacatccac aacgttcagg atcacctgaa agaactggct gaccgttacg
    20641 caatcgtcgc taatgacgta cgcaaagcga ttggcgaagc gaaagatgac gacaccgcag
    20701 atatcctgac cgccgcgtct cgcgacctgg ataaattcct gtggtttatc gagtctaaca
    20761 tcgaataaat ccatcgctga tggtgcagaa ctttagtacc cgataaaagc ggcttcctga
    20821 caggaggccg ttttgttttg cagcccacct caacgcactt atttagtgca tccatctgct
    20881 atccccagct gattaagtaa attttttgta tccacatcat cacacaatcg ttacataaag
    20941 attgtttttt catcaggttt tacgctaaat aatcactgtg ttgagtgcac aattttagcg
    21001 caccagattg gtgccccaga atggtgcatc ttcagggtat tgccctataa atcgtgcatc
    21061 acgtttttgc cgcatctcga aaaatcaatg agttgcaaaa ttggcacgat tttttcatat
    21121 atgtgaatgt cacgcagggg atcgtcccgt ggatagaaaa aaggaaatgc tatgaagtct
    21181 gtattaaaag tttcactggc tgcactgacc ctggcttttg cggtttcttc tcatgccgcg
    21241 gataaaaaat tagttgtcgc gacggatacc gccttcgttc cgtttgaatt taaacagggc
    21301 gataaatatg tgggctttga cgttgatctg tgggctgcca tcgctaaaga gctgaagctg
    21361 gattacgaac tgaagccgat ggatttcagt gggatcattc cggcactgca aaccaaaaac
    21421 gtcgatctgg cgctggcggg cattaccatc accgacgagc gtaaaaaagc gatcgatttc
    21481 tctgacggct actacaaaag cggcctgtta gtgatggtga aagctaacaa taacgatgtg
    21541 aaaagcgtga aagatctcga cgggaaagtg gttgctgtga agagcggtac tggctccgtt
    21601 gattacgcga aagcaaacat caaaactaaa gatctgcgtc agttcccgaa catcgataac
    21661 gcctatatgg aactgggcac caaccgcgca gacgccgttc tgcacgatac gccaaacatt
    21721 ctgtacttca tcaaaaccgc cggtaacggt cagttcaaag cggtaggtga ctctctggaa
    21781 gcgcagcaat acggtattgc gttcccgaaa ggtagcgacg agctgcgtga caaagtcaac
    21841 ggcgcgttga aaaccctgcg cgagaacgga acttacaacg aaatctacaa aaaatggttc
    21901 agtactgaac cgaaataata acgctacacc tgtaaagcgc actggcagtt ccctctcccc
    21961 cctggggaga ggattagggt gaggggcgca aacccgctcc ggggccatta attaccctga
    22021 atttgattat ttacaccacg gtaacaggaa caacatatgc agtttgactg gagtgccatc
    22081 tggcctgcca ttccgcttct gattgaaggt gccaaaatga ccctgtggat ttcggtcctc
    22141 ggtctggctg gcggtctggt aatcggattg ctggcaggtt ttgcacgcac cttcggtggt
    22201 tggatagcca accacgtcgc gctggtcttt attgaagtga tccgcggcac acctatcgtc
    22261 gtccaggtga tgtttatcta tttcgccctg ccgatggcgt ttaacgactt acgcatcgac
    22321 ccatttactg cggcggtggt caccatcatg atcaactccg gcgcgtacat tgcggaaatc
    22381 acgcgtggtg cggtgctgtc tatccacaaa ggttttcgtg aagcaggact ggcgctcggt
    22441 ctttcacgtt gggaaaccat tcgctacgtc attttaccgc tggcactgcg tcgtatgctg
    22501 ccgccgctgg gtaaccagtg gatcatcagc attaaagaca cctcgctgtt tattgttatc
    22561 ggcgtggcgg aactgacccg tcaggggcaa gaaattattg ccggtaactt ccgcgccctt
    22621 gagatctgga gcgccgtggc ggtgttctat ctgattatta ccctggtgct gagctttatt
    22681 ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga atttaaaaac gtctccaagc
    22741 actttggccc aacccaggtg ctgcacaata tcgatttgaa cattgcccag ggcgaagtcg
    22801 tggtgattat cgggccgtcc ggttccggta aatcgaccct gctgcgctgc atcaacaaac
    22861 tggaagaaat cacctccggc gatctgattg tcgatggcct gaaggtcaac gatccgaaag
    22921 ttgacgagcg cctgattcgc caggaagcag gtatggtgtt ccagcagttt tacctcttcc
    22981 cgcatctgac ggcgctggga aacgtcatgt ttggtccgct acgcgtgcgt ggcgcgaaca
    23041 aagaagaggc ggaaaaactg gcgcgtgagc tgctggcgaa agtcggtctg gcagaacgtg
    23101 cacatcacta cccttccgaa ctttctggtg gtcaacagca gcgtgtggcg attgcccgcg
    23161 cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc gacttccgct cttgacccgg
    23221 aactgcgcca tgaagtgctg aaggttatgc aggatctggc tgaagaaggg atgacgatgg
    23281 tgatcgtgac ccacgaaatc ggttttgccg agaaagtagc ttcgcggctg atctttatcg
    23341 acaaaggccg gattgcggaa gatggcaatc cgcaggtgtt gatcaagaac ccgccgagcc
    23401 agcgcttgca ggaatttttg cagcacgtct cttaataaga cacattgcct gatgcgctac
    23461 gcttatcagg cctacaggat atctggcaac ttattaaaat tgcatgaact tgtaggacgg
    23521 ataaggcgtt cacgccgcat ccggcaaaaa agcccgcacg ttgtcagcaa cctgcttaat
    23581 atcccttcct ccctttcacc cgaaagggag gcacaccaga ttcctctcat ttaaaatcgc
    23641 ccctccccca gcatctatac ttatcttttt gctctgtttt ctcactggag gagtcatgcg
    23701 gtggatcctg ttcatcctct tctgcctgct gggcgcacct gcccacgcgg tatccatacc
    23761 cggcgttaca accacaacga caacggactc aacgactgaa ccggccccgg aaccggatat
    23821 cgaacaaaaa aagcggccta tggcgcactg gcggatgtgc tggataacga cacctcgcgt
    23881 aaagagttga tcgaccagtt gcgtactgtc gccgcgacgc cccctgcgga accggtcccg
    23941 aagatcgtgc cgccgacgct ggttgaagag caaaccgtgc tgcaaaaggt caccgaagtc
    24001 agccgccatt atggtgaagc cctttccgcc cgcttcgggc aactttatcg caatatcacc
    24061 ggctccccgc ataagccatt taatccacaa accttcagca atgcgctgag ccatttttca
    24121 atgttagcgg tattagtgtt tggtttttac tggctgattc gcctgtgcgc actgccgcta
    24181 tatcgcaaaa tgggtcagtg ggcgaggcaa aaaaatcgtg agcgcagcaa ctggttgcag
    24241 cttccggcga tgattattgg ggcgtttatt atcgacctgc tgttactggc cctgacatta
    24301 tttgtcggcc aggtattaag cgacaacctg aatgcaggca gtcgcaccat cgctttccaa
    24361 caaagtttgt ttctcaacgc ctttgccctg attgaatttt tcaaagccgt actacgcctg
    24421 attttttgcc caaacgtggc ggagctgcgc ccgttcacga ttcaggacga gagcgcccgt
    24481 tactggggtc gtcgcctgag ctggttaagc agcctgattg gctatggcct gattgtggcc
    24541 gtgccgatta tctctaatca ggtgaatgta cagataggtg cgctggcgaa cgtcatcatt
    24601 atgctgtgca tgaccgtctg ggcgttgtac ctgatcttcc gaaataaaaa agagattacc
    24661 cagcatatgc tcaactttgc ggagcattcg ctggcttttt tcagcctgtt tatccgcgcc
    24721 tttgcgctgg tgtggcactg gctggcaagc gcctatttta tcatgctgtt tttcttttcg
    24781 ttgttcgatc cgggcaacag tctgaaattt atgatgggtg caacggtgcg cagcctggcg
    24841 attattggta tcgcagcgtt tgtttccggt atgttttccc gctggctggc gaaaaccatc
    24901 accctctcgc cacatactca gcgtaactat ccggagctac aaaaacggct gaatggctgg
    24961 ctttcggcgg cgctgaaaac ggcgcgtatt ctgaccgtct gcgtggcagt aatgttgctg
    25021 ttgagcgcat ggggattgtt cgatttctgg aactggctgc aaaacggcgc ggggcagaaa
    25081 accgtagata tcctgatccg catcgcactc attcttttct tctcggcggt tggctggacg
    25141 gtgctcgcca gtttgatcga aaaccggctg gcttcggata ttcatggccg cccgctaccc
    25201 agcgcccgta cgcgtaccct gctgacgctg ttccgtaacg cgctggcggt gattatcagt
    25261 accatcacca tcatgattgt gttgtcggaa atcggcgtca atatcgcgcc attgctggca
    25321 ggtgccgggg cattaggtct ggctatctcg tttggttcgc aaacgctggt aaaagatatt
    25381 atcaccgggg tatttattca gtttgaaaac ggcatgaaca ctggagattt ggtgactatc
    25441 gggccgttga ccggcacagt ggaacggatg tcgattcgct ccgtgggcgt gcgccaggat
    25501 accggggcgt atcacatcat tccgtggtct tcgataacca cctttgctaa cttcgtccgc
    25561 ggtattggtt cggtagtggc aaactacgat gttgatcgcc atgaagatgc tgataaagcc
    25621 aatcaggcac tgaaagatgc ggtaacggaa ttgatggaaa acgaagaaat tcgcgggctg
    25681 attattggtg aaccgaactt tgccgggatt gtcggcttaa gcaataccgc gtttacactg
    25741 cgtgtttcgt tcaccacgct gccactcaaa cagtggacgg tacgctttgc cctcgacagc
    25801 caggtgaaaa aacatttcga cctggcgggc gttcgcgcgc cagtgcagac ttatcaggtg
    25861 ctgcctgctc cgggcgcgac cccggctgaa ccgctgccgc cgggggagcc aacgctttaa
    25921 cgttggcgat taacaaaacg gcggcgctgc tcgtcgttca taaaggtcca ggcaataaag
    25981 cgactctgct tttgcccctg ggccatctct ttttttacca ccttcaccgc gcccacgtcg
    26041 gtcagagcac gatacaacgg cggtaagttt tcaccacgag ataccagcga ggtaaaccac
    26101 atcacctgct tcgcgaagcc tttgctctct tcaatcatct ttttgataaa gttgacttca
    26161 ccgccttcac accacaactc ctgttgctga ccaccaaagt tcagtgcatc gtctttgttc
    26221 agccccaggt tacggcgttt acgctcacta cctgcccgcg ccgcagcggc ggaatcgtgg
    26281 aatggcgggt tacacaaggt cgcgtcgtat tgctcgtttt tgtggatgat gccgttaaaa
    26341 atcgccccac tctctttttg ccgacgcaga cgaatggcgc ggttaagccc cggattagca
    26401 ctgataatcg cctgcgcact gcttaacgcc tggctgctgg tttcactacc ggtaaaacgc
    26461 cagccatatt catgtacgcc aatcagcgga taaatacagt tcgcaccaac gccgatatcc
    26521 agaatgctgg cattcgctgg aattgttccg cttgcttcag ccagtaaatc ggcaaggtga
    26581 tgaatataat ccgcccggcc cggtactggt gggcagagaa aaccgtcggg gatatcccag
    26641 ttcgctacgg cgtaaaaatg ggccagcaac gccttattga gcgccttcac cgccagcgga
    26701 ttggcaaagt ctacgctttg ctcccctgcg ggtgtaagcg tgaggaattg cctgagttca
    26761 ggattgacct gacaaagcgt ggcgagatcg tagcggctgt gatgacggtt gcgcggatgc
    26821 aaccccggtt tctgggcgga catagcattc tcctttaagc agcggcgtaa gatacccgca
    26881 taacgcgaca gggtaaatat ctggctgcgt ttatcattcc ccaaaacctt aacatggcta
    26941 tggtttatgg caattcatca taaaaatcag cattatgcat gtcattgatt tgtcttattt
    27001 tgtaaaaata tatcgttcaa aaaataagca tttctctgtc aattttttgc gtcatttgca
    27061 tcgcgcaggc gacctacact tatcaggcac tacccgttgt atttatgagg atgtcattat
    27121 gaaaaagtgc ctcacactac tgattgccac cgtcctgagc ggaatctctc tcacggctta
    27181 tgccgcgcaa ccgatgagta acctggacag cggtcagcta cggcccgccg gtaccgtttc
    27241 ggcaactggc gcatcaaacc taagcgatct ggaggataaa ctggcagaaa aagcgcgcga
    27301 acaaggcgcg aaaggttatg tcattaattc cgcaggcgga aatgaccaga tgttaggtac
    27361 tgcaaccatc tacaaataac cccaccaacg taactgcatt cgcctctgga tgcagttttc
    27421 gcctgttttg taatcaaaaa acatcaaata tgaacgcaat gtaaataaat gtatttcttt
    27481 ttcgcgcaat gggtgataga aaatcgcatc aagtgataat gcttatcaaa attattatca
    27541 cttgcacgaa cactatcacg ggattaacag tggcatcgca tccgcagaga tgctttctcg
    27601 tggcagtgaa aatttcaaca tataagaaaa agtcacctgc aaaatggaaa acaatcgcaa
    27661 tttccctgcc agacaatttc attcgctcac gttctttgcc ggtctttgta ttggtattac
    27721 gcctgtggca caggcactcg ccgccgaagg gcaagctaac gcggatgaca cgctggttgt
    27781 cgaagcatca acgccttcgc tttatgcgcc acaacaatct gccgatccga aattctcgcg
    27841 tccggtagcg gatactaccc gcacgatgac ggtgatttct gaacaagtga ttaaagatca
    27901 gggcgcaacc aaccttaccg acgcgctcaa aaacgtcccc ggcg
//
LOCUS       DADLTY010000038        27535 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.038, whole genome shotgun sequence.
ACCESSION   DADLTY010000038 DADLTY010000000
VERSION     DADLTY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.038"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="JN334_001390"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="JN334_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HAY7437586.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1958)
                     /locus_tag="JN334_001391"
     CDS             complement(1614..1958)
                     /locus_tag="JN334_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HAY7437587.1"
                     /translation="MFFGGHVLRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAI
                     LSAAGNEYSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGA
                     STGYTVWQLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JN334_001392"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JN334_001392"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAY7437588.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JN334_001393"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JN334_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HAY7437589.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /locus_tag="JN334_001394"
     CDS             complement(6144..6842)
                     /locus_tag="JN334_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HAY7437590.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JN334_001395"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JN334_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HAY7437591.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="JN334_001396"
     CDS             7537..8574
                     /locus_tag="JN334_001396"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY7437592.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JN334_001397"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JN334_001397"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAY7437593.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="JN334_001398"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="JN334_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAY7437594.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JN334_001399"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JN334_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HAY7437595.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="JN334_001400"
     CDS             10745..11626
                     /locus_tag="JN334_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7437596.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="JN334_001401"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="JN334_001401"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HAY7437597.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="JN334_001402"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="JN334_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HAY7437598.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="JN334_001403"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="JN334_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="JN334_001404"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="JN334_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HAY7437599.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="JN334_001405"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="JN334_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HAY7437600.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="JN334_001406"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="JN334_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HAY7437601.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="JN334_001407"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="JN334_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HAY7437602.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="JN334_001408"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="JN334_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HAY7437603.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="JN334_001409"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="JN334_001409"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAY7437604.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="JN334_001410"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="JN334_001410"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAY7437605.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="JN334_001411"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="JN334_001411"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAY7437606.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="JN334_001412"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="JN334_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAY7437607.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="JN334_001413"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="JN334_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAY7437608.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="JN334_001414"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="JN334_001414"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAY7437609.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="JN334_001415"
     CDS             24893..25273
                     /locus_tag="JN334_001415"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAY7437610.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="JN334_001416"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="JN334_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HAY7437611.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="JN334_001417"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="JN334_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HAY7437612.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27535
                     /locus_tag="JN334_001418"
                     /pseudo
     CDS             26442..27535
                     /locus_tag="JN334_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6790 a         6851 c         7222 g         6672 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gattggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 ctttccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgta
//
LOCUS       DADLTY010000039        26863 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.039, whole genome shotgun sequence.
ACCESSION   DADLTY010000039 DADLTY010000000
VERSION     DADLTY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.039"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            231..1664
                     /locus_tag="JN334_001419"
     CDS             231..1664
                     /locus_tag="JN334_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAY7437613.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="JN334_001420"
     CDS             complement(1672..2730)
                     /locus_tag="JN334_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7437614.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="JN334_001421"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="JN334_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="JN334_001422"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="JN334_001422"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HAY7437615.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JN334_001423"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JN334_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAY7437616.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JN334_001424"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JN334_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HAY7437617.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="JN334_001425"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="JN334_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HAY7437618.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="JN334_001426"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="JN334_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAY7437619.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="JN334_001427"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="JN334_001427"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HAY7437620.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="JN334_001428"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="JN334_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HAY7437621.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="JN334_001429"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="JN334_001429"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HAY7437622.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="JN334_001430"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="JN334_001430"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HAY7437623.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="JN334_001431"
     CDS             complement(17699..18322)
                     /locus_tag="JN334_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HAY7437624.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="JN334_001432"
     CDS             18619..18894
                     /locus_tag="JN334_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437625.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="JN334_001433"
     CDS             complement(18971..19213)
                     /locus_tag="JN334_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HAY7437626.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="JN334_001434"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="JN334_001434"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HAY7437627.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="JN334_001435"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="JN334_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HAY7437628.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="JN334_001436"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="JN334_001436"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAY7437629.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22592..24078
                     /locus_tag="JN334_001437"
                     /pseudo
     CDS             22592..24078
                     /locus_tag="JN334_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24093..24827
                     /locus_tag="JN334_001438"
     CDS             24093..24827
                     /locus_tag="JN334_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7437630.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24840..25697
                     /locus_tag="JN334_001439"
                     /pseudo
     CDS             24840..25697
                     /locus_tag="JN334_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25700..>26863)
                     /locus_tag="JN334_001440"
     CDS             complement(25700..>26863)
                     /locus_tag="JN334_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAY7437631.1"
                     /translation="IGGDKSIPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILR
                     AASFGNAQLMIPMVHSLDQILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCY
                     IIDHFCDEVDFFSIGSNDMTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQR
                     GKWVGICGELGGESRYLPLLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQAC
                     ECRSAQEIEALLTAFTPEEDVRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEH
                     PFELEEDVWQREEIVTTGVGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVEL
                     VIMLTLGANEGMNHVKVFSQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
BASE COUNT         7422 a         6361 c         5976 g         7104 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caagattaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattaattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttt aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct accgctgttt
    23041 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
    23101 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
    23161 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
    23221 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg
    23281 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
    23341 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
    23401 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
    23461 attcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
    23521 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
    23581 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
    23641 tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa gctgacggtt
    23701 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
    23761 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
    23821 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
    23881 gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
    23941 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
    24001 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
    24061 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
    24121 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
    24181 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
    24241 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
    24301 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
    24361 cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
    24421 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
    24481 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
    24541 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
    24601 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
    24661 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
    24721 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtgccgg
    24781 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
    24841 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctag
    24901 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
    24961 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
    25021 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
    25081 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
    25141 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
    25201 gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
    25261 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
    25321 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg cgcggcgatt
    25381 ttctctatca cagcatttgt aactgggttt aggataatta tgcccagccg ctcacccgcg
    25441 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
    25501 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
    25561 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
    25621 gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga gagtacagcg
    25681 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatacttt
    25741 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
    25801 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatca
    25861 ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc cgggcaatgc
    25921 tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa
    25981 cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca aacggatgtt
    26041 cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc tctttattgc
    26101 taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg tcttcttccg
    26161 gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca catgcctgac
    26221 gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc accgccggaa
    26281 tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc ggcagataac
    26341 ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg
    26401 taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat agcggcgata
    26461 cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg gagccgatac
    26521 tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac ggcacttcca
    26581 ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta agctcaacga
    26641 tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtga accatcggga
    26701 tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga
    26761 acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac gggttctctt
    26821 cctggggaat attcagataa ggaatgcttt tatcgccgcc gat
//
LOCUS       DADLTY010000040        26663 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.040, whole genome shotgun sequence.
ACCESSION   DADLTY010000040 DADLTY010000000
VERSION     DADLTY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.040"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..918
                     /gene="metL"
                     /locus_tag="JN334_001441"
     CDS             <1..918
                     /gene="metL"
                     /locus_tag="JN334_001441"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HAY7437632.1"
                     /translation="SYDGLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVT
                     ASQQLADQYLDFASHGFHVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGL
                     PINHTVRDLIDSGDTILSISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPR
                     DDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMV
                     QRLEAAREMGLVLRYVARFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDN
                     PLVIRGPGAGRDVTAGAIQSDINRLAQLL"
     gene            1267..2157
                     /gene="metF"
                     /locus_tag="JN334_001442"
     CDS             1267..2157
                     /gene="metF"
                     /locus_tag="JN334_001442"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HAY7437633.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            2486..4666
                     /gene="katG"
                     /locus_tag="JN334_001443"
     CDS             2486..4666
                     /gene="katG"
                     /locus_tag="JN334_001443"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAY7437634.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            4727..5665
                     /gene="yijE"
                     /locus_tag="JN334_001444"
     CDS             4727..5665
                     /gene="yijE"
                     /locus_tag="JN334_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HAY7437635.1"
                     /translation="MSTGKLHTGSLMSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVT
                     SYIGAFDFTALRCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWAL
                     VSGGAGKVAILSYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFS
                     SMKSAMLAILSGVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQ
                     REIDWQPTVFWALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWL
                     LGENPGAVEGSGIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(5692..6299)
                     /locus_tag="JN334_001445"
                     /pseudo
     CDS             complement(5692..6299)
                     /locus_tag="JN334_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(6573..7674)
                     /gene="gldA"
                     /locus_tag="JN334_001446"
                     /pseudo
     CDS             complement(6573..7674)
                     /gene="gldA"
                     /locus_tag="JN334_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            complement(7685..8347)
                     /gene="fsa"
                     /locus_tag="JN334_001447"
                     /pseudo
     CDS             complement(7685..8347)
                     /gene="fsa"
                     /locus_tag="JN334_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(8359..10860)
                     /gene="ptsP"
                     /locus_tag="JN334_001448"
                     /pseudo
     CDS             complement(8359..10860)
                     /gene="ptsP"
                     /locus_tag="JN334_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            11169..12248
                     /locus_tag="JN334_001449"
     CDS             11169..12248
                     /locus_tag="JN334_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAY7437636.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            12263..12583
                     /locus_tag="JN334_001450"
     CDS             12263..12583
                     /locus_tag="JN334_001450"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY7437637.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            12634..14931
                     /locus_tag="JN334_001451"
     CDS             12634..14931
                     /locus_tag="JN334_001451"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY7437638.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            14897..15775
                     /locus_tag="JN334_001452"
     CDS             14897..15775
                     /locus_tag="JN334_001452"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HAY7437639.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            15777..16118
                     /locus_tag="JN334_001453"
     CDS             15777..16118
                     /locus_tag="JN334_001453"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY7437640.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(16105..16956)
                     /locus_tag="JN334_001454"
     CDS             complement(16105..16956)
                     /locus_tag="JN334_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7437641.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(17105..18838)
                     /gene="cptA"
                     /locus_tag="JN334_001455"
     CDS             complement(17105..18838)
                     /gene="cptA"
                     /locus_tag="JN334_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HAY7437642.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(19021..21672)
                     /gene="ppc"
                     /locus_tag="JN334_001456"
     CDS             complement(19021..21672)
                     /gene="ppc"
                     /locus_tag="JN334_001456"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAY7437643.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(22024..23175)
                     /gene="argE"
                     /locus_tag="JN334_001457"
     CDS             complement(22024..23175)
                     /gene="argE"
                     /locus_tag="JN334_001457"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAY7437644.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            23329..24333
                     /gene="argC"
                     /locus_tag="JN334_001458"
     CDS             23329..24333
                     /gene="argC"
                     /locus_tag="JN334_001458"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAY7437645.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            24341..25117
                     /gene="argB"
                     /locus_tag="JN334_001459"
     CDS             24341..25117
                     /gene="argB"
                     /locus_tag="JN334_001459"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAY7437646.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            25178..26551
                     /gene="argH"
                     /locus_tag="JN334_001460"
     CDS             25178..26551
                     /gene="argH"
                     /locus_tag="JN334_001460"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAY7437647.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
BASE COUNT         6047 a         6734 c         7441 g         6441 t
ORIGIN      
        1 agttatgacg ggctggacgc cagccgcgcg ttagccttct tcaacgatga agcggtcgag
       61 caggatgaag agtcgctttt cctgtggatg cgcgcccatc cgtatgatga tttagtggtg
      121 ttggacgtta ccgccagcca gcagcttgct gatcagtatc tcgatttcgc cagccacggt
      181 ttccacgtca tcagtgccaa caaactggcg ggagcaagcg acagcaataa atatcgccag
      241 atccacgacg ccttcgaaaa aaccgggcgt cactggctat acaacgccac cgttggtgcg
      301 ggcttgccga tcaaccatac cgtgcgcgat ctgatcgaca gcggcgatac cattttgtcg
      361 atcagtggga tcttctccgg cacgctctcc tggctgttcc tgcaattcga cggtagcgtg
      421 ccgtttaccg agctggtgga tcaggcgtgg cagcagggct taaccgagcc tgatccgcgt
      481 gacgacctct ccggcaaaga cgtaatgcgc aagctggtga ttctggcgcg tgaagcgggt
      541 tacaacattg aaccggacca ggtacgtgtg gaatcgctgg tgcctgctca ttgcgaaggc
      601 ggcagcatcg accatttctt tgaaaatggc gatgaactta acgagcagat ggtgcaacgg
      661 ctggaagcgg cccgcgaaat ggggctggtg ctgcgctacg tggcgcgttt cgatgccaac
      721 ggcaaagcgc gtgtgggcgt ggaggcggtg cgtgaagact atccgctggc atcactgctg
      781 ccatgcgata acgtctttgc catcgaaagc cgctggtacc gcgataatcc gctggtgatc
      841 cgtgggcctg gcgctgggcg cgacgtcacc gccggggcga ttcagtcgga tatcaaccgg
      901 ctggcacagt tgttgtaatt tcagaaattt aataatgccc ggtactcatg ttttcgggtc
      961 tatggtttct aatgaaatat attgaattat catagaatta ggccggatga agcgtttacg
     1021 acgaatccgg caagaagcaa taagtacatg gttagtttat atttgcagtc cggtttgctt
     1081 tgcatatcgg attttctttt tcttaccatc ctgaagtttt ttcatcttcc ctgatttttc
     1141 ctcaccatca ttggtcattt ttcggttgac gcccttcggc ttttccttca tctttacatc
     1201 tggacgtcta aatggataga tgtgcacaac acaacatata actacaagcg attgatgagg
     1261 taaggtatga gcttttttca cgccagccag cgggatgccc tgaatcagag cctggcagaa
     1321 gtccaggggc agattaacgt ttcgttcgag tttttcccgc cgcgtaccag tgaaatggag
     1381 cagaccctgt ggaactccat cgatcgcctt agcagcctga aaccgaagtt tgtatcggtg
     1441 acctatggcg cgaactccgg cgagcgcgac cgtacgcaca gcattattaa aggcattaaa
     1501 gatcgcactg gtctggaagc ggcaccgcat cttacttgca ttgatgcgac gcccgacgag
     1561 ctgcgcacca ttgcacgcga ctactggaat aacggtattc gtcatatcgt ggcactgcgt
     1621 ggcgatctgc cgccgggaag tggtaagcca gaaatgtatg cttctgacct ggtgacgctg
     1681 ttaaaagaag tggcagattt cgatatatcc gtggcggcgt atccggaagt tcacccggaa
     1741 gcaaaaagcg ctcaggcgga tttgcttaat ctgaaacgca aagtggatgc cggagccaac
     1801 cgcgcgatta ctcagttctt cttcgatgtc gaaagctacc tgcgttttcg tgaccgctgt
     1861 gtatcggcgg gcattgatgt ggaaatcatc ccgggcattt tgccggtatc taactttaaa
     1921 caggcgaaga aatttgccga catgaccaac gtgcgtattc cggcgtggat ggcgcaaatg
     1981 tttgacgggc tggatgacga tgccgaaacc cgcaaactgg tgggcgcgaa tattgccatg
     2041 gatatggtga agattttaag ccgtgaagga gtgaaagatt tccacttcta tacgcttaac
     2101 cgcgctgaaa tgagttacgc gatttgccat acgctgggtg ttcgacctgg tttataaata
     2161 gtgtggcttt tgtgaaaatc acacagtgat cacaaatttt aaacagagca caaaatgctg
     2221 cctcgaaatg agggcgggaa aataaggttg tcagccttgt tttctccctc attacttgaa
     2281 ggatatgaag ctaaaaccct tttttataaa gcatttgtcc gaattcggac ataatcaaaa
     2341 aagcttaatt acgatcaatt tgatctacat ctctttaacc aacaatatgt aagatctcaa
     2401 ctatcgcatc cgtggattaa ttcaattata acttctctct aacgctgtgt atcgtaacgg
     2461 taacactgta gaggggagca cattgatgag cacgtcagac gatatccata acaccacagc
     2521 cactggcaaa tgcccgttcc atcagggcgg tcacgaccag agcgcggggg cgggcacaac
     2581 cactcgcgac tggtggccaa atcaactccg tgttgacctg ttaaaccaac attctaatcg
     2641 ttctaacccg ctgggtgagg actttgacta ccgcaaagaa ttcagcaaat tagattacta
     2701 cggcctgaaa aaagatctga aagccctgct gacagaatct cagccgtggt ggccagccga
     2761 ctggggcagc tacgccggtc tgtttattcg tatggcttgg cacggcgcag gtacttaccg
     2821 ttcaatcgat ggacgcggcg gcgcgggtcg tggtcagcaa cgttttgcac cgctgaactc
     2881 ctggccggat aacgtaagcc tcgataaagc gcgtcgcctg ttgtggccaa tcaaacagaa
     2941 atatggtcag aaaatctcct gggccgacct gtttatcctc gcaggtaacg tggcgctgga
     3001 aaactccggc ttccgtacct tcggttttgg tgccggtcgt gaagacgtct gggaaccgga
     3061 tctggatgtt aactggggtg atgaaaaagc ctggctgact caccgtcatc cggaagcgct
     3121 ggcgaaagca ccgctgggtg caaccgagat gggcctgatt tacgttaacc cggaaggccc
     3181 ggatcacagc ggcgaaccgc tttctgcggc agcagctatc cgcgcgacct tcggcaacat
     3241 gggcatgaac gacgaagaaa ccgtggcgct gattgcgggt ggtcatacgc tgggtaaaac
     3301 ccacggtgcc ggtccgacat caaacgtagg tcctgatcca gaagctgcac cgattgaaga
     3361 acaaggttta ggctgggcga gcacttacgg cagcggcgtt ggcgcagatg ccattacctc
     3421 tggtctggaa gtagtctgga cccagacgcc gatccagtgg agcaactatt tcttcgagaa
     3481 tctgttcaag tatgagtggg tacagacccg cagtccggct ggagcaatcc agttcgaagc
     3541 ggtagacgca ccggaaatta tcccggatcc gttcgatccg tcgaagaaac gtaaaccgac
     3601 aatgctggtg accgacctga cgctgcgttt tgatcctgag ttcgagaaga tctctcgtcg
     3661 tttcctcaac gatccgcagg cgttcaacga agcctttgcc cgtgcctggt tcaaactgac
     3721 gcacagggat atggggccga aatctcgcta catcgggccg gaagtgccga aagaagatct
     3781 gatctggcaa gatccgctgc cgcagccgat ctacaacccg accgagcagg acattatcga
     3841 tctgaaattc gcgattgcgg attctggtct gtctgttagt gagctggtat cggtggcctg
     3901 ggcatctgct tctaccttcc gtggtggcga caaacgcggt ggtgccaacg gtgcgcgtct
     3961 ggcattaatg ccgcagcgcg actgggatgt gaacgccgca gccgttcgtg ctctgcctgt
     4021 tctggagaaa atccagaaag agtctggtaa agcctcgctg gcggatatca tcgtgctggc
     4081 tggtgtggtt ggtgttgaga aagccgcaag cgccgcaggt ttgagcattc atgtaccgtt
     4141 tgcgccgggt cgcgttgatg cgcgtcagga tcagactgac attgagatgt ttgagctgct
     4201 ggagccaatt gctgacggtt tccgtaacta tcgcgctcgt ctggacgttt ccaccaccga
     4261 gtcactgctg atcgacaaag cacagcaact gacgctgacc gcgccggaaa tgactgcgct
     4321 ggtgggcggg atgcgtgtac tgggtgccaa cttcgatggc agcaaaaacg gcgtcttcac
     4381 tgaccgcgtt ggcgtattga gcaatgactt cttcgtgaac ttgctggata tgcgttacga
     4441 gtggaaagcg actgacgaat cgaaagagct gttcgaagga cgtgaccgcg aaaccggcga
     4501 agtgaaatac acagccagcc gtgcggatct ggtatttggt tctaactctg tcctgcgtgc
     4561 agtagcggaa gtttacgcca gcagcgatgc ccacgagaag tttgttaaag acttcgtagc
     4621 ggcatgggtg aaagtgatga acctcgaccg tttcgacctg ctgtaatctg accttcttca
     4681 gcgactgtct ttcaggcagt cgctgaagtt tctttaccgg cgtatagtgt ccacaggaaa
     4741 actacacact ggatctctca tgtctgccgc aggaaagagc aacccactgg caatcagtgg
     4801 cttggttgtg ctcacactta tctggagtta tagctggatt ttcatgaagc aagtcaccag
     4861 ttacatcggt gccttcgact ttaccgcctt acgctgcatt ttcggcgctc tcgttttatt
     4921 catcgtcctt ttattacgtg gtcgcggaat gcgcccgacg ccgtttaaat acaccttagc
     4981 cattgccctg ttacaaacct gcgggatggt tggtctggcg cagtgggcgt tggtcagcgg
     5041 aggtgcgggg aaggtggcga tcctgagcta taccatgccg ttctgggtgg tgattttcgc
     5101 cgcgttgttt ctcggtgaac gcctgcgacg tgggcaatat ttcgcgattc tgattgccgc
     5161 tttcggctta tttttggtgt tgcagccgtg gcaactcgat ttctcttcga tgaaaagtgc
     5221 catgctggca atcctctccg gtgttagttg gggggcgagc gcgattgttg ctaaacgcct
     5281 atacgcccgt catccgcgcg tggatttatt atcgttaaca tcctggcaga tgctgtatgc
     5341 ggcgctggtg atgagtgtgg tcgctttact ggtgccgcaa cgtgaaattg actggcagcc
     5401 caccgtgttc tgggcgctgg cctacagtgc gattctggcg acggcgctag cgtggagctt
     5461 atggctgttt gtgctgaaaa acttgcctgc cagtattgcc agcttaagca cactggccgt
     5521 tcccgtttgt ggcgtactct tttcctggtg gctgctcggt gagaatccgg gggccgttga
     5581 aggtagcggt attgtgctga ttgtgctggc actggcgctg gtgagccgta agaaaaaaga
     5641 agctgtcagt gtaaaaagca tctgaatttt ttcttcatgt ggggcgatct cttatttaac
     5701 aaaataacga taatgcccca ccatccgcca gctaaacagc acatcttctt cctgcgcgcc
     5761 tgcgccaatg ttatgtatca ccagcggcgt accgtcgcgg gcgaagccat ctgacgccac
     5821 cccaatatgc gccagcccgt tatctaatcg ccaggagaca atatcgcccg cttgatagtc
     5881 accggcattt ttactgatgg ggcgtgtttt gttgtggcgg ctaaaccagg tttccagatt
     5941 aggcacccga cggtgatcga tgttgctgtc cgggcgcttt aacttccatt tttgcgggta
     6001 ctcagcaaaa ttcttcgcca tatcttcgtg aaccagtttc tgcaaatcga ccttctggct
     6061 gcgcaatgcg cggatcacca catcggaaca tacaccgcgt tcttgcggaa catcaccgcc
     6121 aggataggta agctgcgatc gtaaaatagc gtgctgccaa tttgctgtct ggcaccgtct
     6181 gcgatggcaa ggttggtatt ggcctggatt tgtaccacgg ttggtggaac ggcgggagat
     6241 tttaaggagt ggctggtaaa tgccgttagc aggctgagca gcgccagtga agctttcatc
     6301 tggttcgtcc gtgaaaataa gagtcacaga ttcgaccttc cgggctaacg attgttctgt
     6361 ggagaaaatg tccagaagcg gataaaaaac tcctgaacaa cgtgggagtt tcagattact
     6421 ggcacagagt gccggatgcg gagtgaacgc cttatccggc ctacaaagca cgcaaattca
     6481 atatattgca gagattgcgt aggcctgata agcgtagcgc atcaggcaat tttgcgttca
     6541 aactcccgga caagccggga gtttggagta ggttattccc actcttgcag gaaacgctga
     6601 ccgtactggt cagctaccag cagtgcggcg taaacctgat ctggcgtcgc gccgccaggc
     6661 atgttgtgga tggtttcacc ttctgcacaa gatgcttttg caacaatgcg cattttcgcc
     6721 gggacatctt ctttaatatc cagttgagcg agagttattg gcaaacctac cgcatggcta
     6781 agtgcagcta cggtttcgat ttcctccacc ggcgcgtttt ccagtaccag ctgcgtcagc
     6841 gtaccaaatg ccacttttca ccgtgataat agtgatgcgc gtccgggata gcggtcaggc
     6901 cgttatgcac cgcgtgagcc gccgccagcc gccgctttca aaaccaacgc cgctcaaata
     6961 ggtgttcgct tcaatcacgc gctccagcgc cggggttact acatgctgtt cggcggcaag
     7021 catcgctttt tcgccttctt ccagcagggt gttgtagcac agttcagcca gtgccagcgc
     7081 cgcctgggtg cacttgccgc ccgccatggt ggtcgcgccg ctacgagagc aagcacgcgc
     7141 ttcaaaccag gttgccagcg catcgccgat acccgctgct aacagacgtg caggtgcgcc
     7201 cgcgacgatt ttggtgtcga caatgaccat attcgggtta tttggcaaca gcagatagcg
     7261 gtcaaactca ccctcatcgg tgtagataac agacaatgcg ctgcacggtg catcggtcga
     7321 ggcgatagtc ggtgcgatcg ctaccggaac acccatgaaa tgtgccagtg ctttggcagt
     7381 atcgagggtt tttccgccac cgataccgag aattgcgcca cactgcgcag tctccgcgat
     7441 gccacgcaga cggtcgatct cattttgcga acattcaccg ccaaacggcg taatttctac
     7501 taccagtcca gcatctttaa agcttttctc gacagtggat tgagcaaaaa ctaaaacaaa
     7561 tttgtcaccc accactaacc agcgttctgc cagcggcttc aggtattcgc ccagacgatt
     7621 aatcacatca gcgccctgga tgtatttacc cggtgattga ataatgcggt ccataattgc
     7681 tcctttagag atgagtagtg ccaaatgcgg cattccagtc gtgttcgaac ttctctatag
     7741 ctgactctac cgcaggggtg ttgagcattt gttgcgctac atctaagggc agggtgatgg
     7801 attcacatcc tgccagtaaa cagtccagcg cctgacgcgg cgttttaaag ctggctgcca
     7861 gcaccatgct ttctggcgcg tgcatttcta acagcgtttg cagctcctga accgtacgaa
     7921 tgccgtcccc gccctgggca tctacgcggt taacatacgg agcaacgtat tttgcccctg
     7981 ccagtgcggc taataaccct tctgcggcgc tatatacagc ggtgccaagt gtagtaatac
     8041 cctatttttt cagtatttta attgctgcca gaccttcgga agtcaccggg attttcacca
     8101 caatacccgg aatagcgtcg cgcaggcgct tcgcttcttc caccatcccc tgcgcgtcgc
     8161 ggctcatggt ctgagcaaac agaatgccct catcaccaat cgctttttgc agacgcggca
     8221 gcacttccca gatggactcc ttgctggcag cgataatgct cgggttagtt gtcaccccgg
     8281 caatcgggaa tatgcgtgcc agacgttcga cttctgcgac gttagcggtg tccagataca
     8341 gttccatgat gtttcctttt acagttccag ttcatgttgc agcaggctgg cgatagcgtc
     8401 ggcagaggcg gcgttaacca gcgcgttacg gaattcttcg tgcatgatgc ggcgagcgag
     8461 gcgcgagaaa atgcgcatgt gctgatcgcc cgcagcgtgt ttgttcaggg ttaacataat
     8521 gatgaattgc gcttcatcat cgccccagcg caccggcgct tgcagacgcg ccacgctgat
     8581 ggtggattgc tcaatgtgtt ctgatttgct gtgtggaatg gcaaaactaa agcccagacc
     8641 ggtagagaaa acggcctcgc gcgcccataa gtcggcttcc agtttacgcg gatagcggca
     8701 gcggcccgcc agcagcaggt tatcggtcat gcctttgagc acttcttctt tgctgcgcca
     8761 gtcactttcc agcgtgatgc actcggcggt gaccagcggt gcgtcctgtt gggtcatgcg
     8821 gaattgcgcc agcaggtgtt ccacttccag cgaagtacgg caggccattg cctggttgag
     8881 caacttgcgg cactcacggc tatcaagttg cgccatccgc gctttcgccg ccggaattga
     8941 tggtgcgctc atgctgagtt catccagccc taagccgacc agcaacggca gcacggaacc
     9001 tttcgctccc agctcaccgc acagaccaat ccatttaccc tggcgatgca ccgcctgcac
     9061 gtcgtaatcg agcgcccgca agaatgccgg attcaggctg ttgtagtgac gagtaacctt
     9121 agcgttatcg cgatccactg ccagcagata ttgcgtcagg tcattactac caatactaaa
     9181 gaaatcaatc tcttcgcagc attgatcgat gatgaacatc accgacggca cttccagcat
     9241 aatgccgagc tggatcttct catcaaacgg aatgtgttcg ttacgtagtt gctgtttggc
     9301 ttctgccagt ttttctttca cccataagat ctcttccatt gaggagatca tcgggatcat
     9361 gattttcagg ctgccgtgag cggaggcgcg caggatcgag cgtagttgtg tggtaaataa
     9421 cgacgcgtat tcttcataaa tacgcacggc gcgataaccg aggaacgggt ttgcctctgc
     9481 gggaatgttt agataatcaa cgggtttgtc gccgccaatg tccatagtgc gcacaataat
     9541 gctgcgtccg ttggcagact ccagcgcctg acaaaaaatg ttgtacagct cgctttcgcc
     9601 cggtgcactg gtgcgatcca tatagagcat ttcagtgcgg aacaaaccaa cgccttccgc
     9661 accattgccg aatgcggcct gcgcttccac ggagtgagcg atgttagcgg caatttcaat
     9721 gcggataccg tcagcggtac gggcttgttg ggtcagctag acacgctgtt gctcacgcag
     9781 ggcgtcctgt acgcgggctt cttgctgata ataacgagct acggcttctc ctggctcaac
     9841 cacaatcgcc ccggcgttgc cgtcgatata aatcgtttga tgctgccacg gagtgagggc
     9901 atcaatatcc acaccaacca gcgtcggaat gttgaacgaa cgggcaagga tcaccgtatg
     9961 tgaggtggtg ccgccgcttt tcagtaacaa tcctttgagg tgatttttat ccagttcgag
    10021 gaactggctg ggggtcagtt catcagccat acaaatggcg gactgcgtca gtttgcccgg
    10081 tgccgggaag cgttgctcac cgtagatttg ctggagtaac tggaagcaga cgtcgcgtac
    10141 gtccagggca cgttcttgca ggtatctgct gctggaacgg gcaaactctt cgcaaaagtg
    10201 attcgcactg gcgacaatcg cttcggcgca gcttaatccg gcgctgacgc ctgccagtaa
    10261 atgttcgtgc agggaagtat cgccagccag ggatcggtgc gcttccagaa tcgcgctggt
    10321 agcaccgtcg ctatccagca gacgaaactc aatgtttttc agtaccagcg tcaggccgtt
    10381 ttccagtgcg gattgctcgg cgtcaacgtc tttggctgcg ggaagattac tcagcgcatt
    10441 gagatctaaa gaagagatcg gcgtcagaat gccgcctgca ctaccgctgc acacggtgcg
    10501 ggcgcggata atttgcggat tcagattggt cagtgaaacc ggcagtggtt ccagttcgtc
    10561 agatttaact tccgccagcg gcgcatcgca gtgggggaat tcatcgcgca gccattggct
    10621 taaacgctgg tgcccttcct gttcgtcggc cccggaaatc agtagctggc agttatcacc
    10681 caccagcgta tcggtgccaa tcagcgccag agcgcttttg gcgttgcctt tacggtcagt
    10741 acgcaggtta tgccactcaa tttgagatga aaaagtatta cacagcgttt caacgtggct
    10801 tgccggacgc gcatgtacgc cgttaggtag ctcacaaata aattccacaa tcagggccat
    10861 tgcctttctc ccggtacggt tttctgttta caggataaga gcgcacggta acggcctgcc
    10921 atgtgacaaa tctgccaaaa gctggacaaa tgtaatgtaa ccgtcaattt gcgacgcgtc
    10981 tcacaagacg ccgttttgcg gcatgcttcc ggattatcgc aagttatgag gcggatcgca
    11041 tttttgtact gatattacaa aagtccagta aatggccttt ttatccactg tttgctccgc
    11101 ctgcgattgc ctattgtttc ctccatcaaa aatatggaca tgggcttttg caagcccggg
    11161 agcagcttat gaatgagttg gtgcagatcc tgaaaaatac ccgtcagcat ttaatgacgg
    11221 gcgtttcaca catgattccc ttcgtggtat cgggcggtat tttgctggcg gtttccgtca
    11281 tgttgtatgg caaaggcgca gtgccggatg ccgtagccga tccgaatttg aaaaaactgt
    11341 ttgatatcgg cgttgcaggt ttgacgctga tggtgccttt cctcgccgct tacattggtt
    11401 actccattgc agagcgttct gcgctggctc cgtgcgctat cggtgcctgg gttggtaaca
    11461 gctttggtgc gggcttcttt ggtgcgctga tcgccgggat tatcggcggc atcgtggtgc
    11521 attacctgaa gaaaattccg gtgcataaag ttctgcgctc ggtgatgccc atcttcatta
    11581 ttcctatcat cggcacactg attaccgcag gcatcatgat gtggggcttg ggcgagcctg
    11641 taggggcgtt gaccaacagc ctgactcagt ggcttcaggg gatgcagcag ggcagcattg
    11701 ttatgctggc ggtgatcatg gggctgatgc tggcgttcga tatgggcggt ccggttaaca
    11761 aagtggccta tgccttcatg ctgatttgcg ttgctcaggg tgtttatacc gtggtggcca
    11821 ttgctgccgt gggtatttgt atcccgccgc tggggatggg gctggcgacg ctgattggtc
    11881 gtaaaaattt ctccgcagaa gagcgcgaaa ccggcaaagc ggcactggtg atgggctgtg
    11941 ttggcgttac tgaaggggcg attcctttcg ccgctgccga tccgctgcgt gtcattcctt
    12001 ccattatggt cggttcagtt tgtggtgcag taactgcggc gctggtcggt gcgcagtgct
    12061 atgcaggctg gggtggtctg attgtgctgc cggtggttga aggtaagttg ggttatatcg
    12121 cagcagtggc tgtcggagca gtggtgacgg ctgtttgtgt gaacgtgctg aaaagtctgg
    12181 cgcgtaaaaa tgggtcttcg actgatgaaa aagaagacga cctggatttg gattttgaaa
    12241 ttaattaatt gaggaacgag ccatgacgaa aattattgca gtaaccgcat gtccttcagg
    12301 tgttgcccat acctatatgg cagcagaggc gctggaaagt gccgcgaaag cgaaaggctg
    12361 ggaagtgaaa gtagaaaccc aggggtcaat tggtctggaa aatgaactga ctgcggaaga
    12421 cgtggcgagc gctgatatgg tgattctgac caaagatatc ggcatcaagt ttgaagagcg
    12481 ttttgctggc aagaccatcg tgcgcgtcaa catcagcgat gcagtaaaac gtgctgacgc
    12541 cattatgagc aaaattgaag ctcacctggc gcaaaccgct taagttcttc tcccgctcgc
    12601 aagggcgggt tcgctttccc acaggagttc ctcatgacga atcgtatctc tcgcctcaaa
    12661 actgcactgt ttgccaatac ccgtgaaatc tcgctggagc gggcgctgct ttataccgcc
    12721 agccatcggc aaaccgaagg cgaaccggtg atattgcgcc gggcgaaagc aacagcgtat
    12781 atccttgaac atgttgaaat ttcgattcgt gatgaagaac tgattgccgg taaccgcacc
    12841 gtaaaaccgc gcgccgggat tatgtcgccg gaaatggacc cttactggct gctgaaagag
    12901 ctggatcaat tcccgacgcg tccgcaggac cgctttgcta tcagcgaaga agataaacgt
    12961 atctaccgtg aagagttgtt cccgtactgg gaaaaacgtt cgatgaaaga tttcatcaac
    13021 gggcagatga cagatgaagt aaaagccgcg accaacacgc agattttcag catcaaccag
    13081 acggataaag gccaggggca cattattatt gattacccac gcctgttgaa tcacggtctg
    13141 ggtgagctgg tggcacagat gcagcaacat tgtcagcaac agccggagaa tcacttttat
    13201 caggccgcgt tgttactgct ggaagcctcg cagaaacaca ttttgcgtta cgccgaactg
    13261 gcggaaacga tggcggcaaa ctgcacagat gcccagcgtc gcgaagagct gctgactatt
    13321 gcggagatct cccgccataa cgcgcaacat aagccgcaga cgttctggca ggcgtgccag
    13381 ttattctggt acatgaacat cattctgcaa tacgaatcca acgccagttc gctgtcttta
    13441 gggcgcttcg accagtatat gttgccgttc tatcaggcat ctttaaccca gggcgaagat
    13501 ccggcgttcc tgaaagaact gctcgaatct ttatgggtga aatgcaacga catcgtgctg
    13561 ttgcgctcca ccagtagcgc gcgttatttc gccggtttcc cgaccggcta taccgcactg
    13621 ctcggcgggt taaccgagaa cggacgtagc gcggtgaacg tgctttcgtt cctttgcctt
    13681 gacgcctatc aaagcgtgca attaccgcaa ccgaacctcg gcgtgcgcac taacgccttg
    13741 atcgacacgc cgttcctgat gaaaaccgcc gaaaccattc gcctcggcac cggtattccg
    13801 caaatcttta acgatgaagt ggtggtgcca gcgttcctca accgtggcgt ttcgctggaa
    13861 gatgcgcgcg actattccgt agtgggctgt gtggaattat ctattcccgg cagaacctac
    13921 ggcttgcatg acatcgcgat gtttaatctg ctgaaagtga tggaaatctg cctgcatgaa
    13981 aatgaaggca atgctgcgct gacttatgaa ggtttactgg agcagatccg cgccaagatc
    14041 agccactaca tcaccctgat ggttgagggc agcaatattt gtgatatcgg ccatcgcgac
    14101 tgggcacctg taccgctgct ctcgtctttt atcagcgatt gtctggaaaa aggtcgcgat
    14161 attaccgatg gcggcgcgcg ttataacttc tccggcgtac agggaattgg tatcgccaac
    14221 ctgagcgatt ctctccatgc gttgaaaggg atggtttttg agcaacagcg tttaagtttt
    14281 gacgaattgc tgtcggtatt aaaagccaac tttgcaacgc cagaaggcga aaaagtccgc
    14341 gctcgcttaa ttaaccgctt tgagaaatac ggtaacgata tcgacgaggt ggataacatt
    14401 agcgccgaac tgttgcgcca ctactgcaaa gaagtggaaa aataccagaa cccgcgcggc
    14461 ggttacttca cgccgggatc gtataccgtt tctgctcacg ttccgttggg atcggtggtt
    14521 ggcgcgacgc cagacggtcg ttttgccggg gaacaactgg cagacggcgg cttgtcacct
    14581 atgctgggtc aggacgcaca agggccaacg gcggtactga agtcagtcag taagctcgat
    14641 aatacgctgc tgtctaacgg tacgttgctg aacgtgaaat tcactccggc gaccctggaa
    14701 ggtgaagcgg gattacgcaa actggccgac ttcttacggg tgtttaccca gcttaagttg
    14761 cagcatattc agtttaacgt ggtgaacgcc gacacgttgc gggaagcgca acagcgccca
    14821 caagattatg ccgggctggt ggtgcgcgtt gccggataca gcgccttctt tgtcgaactg
    14881 tcgaaggaga tccaggatga catcatccgc cggacagcgc atcagctgta acgttgtgga
    14941 aacgcgccgc gatgatgtgg cgcgcatttt caacattcag cgttattcac tgaatgacgg
    15001 cgagggcatt cgtacggtgg tcttttttaa aggctgtccg catctttgcc cgtggtgtgc
    15061 taatccggag tcgatctccg gcaaaatcca gacggtacgc agagaggcga aatgtctgca
    15121 ctgtgcgaaa tgtttgcgtg atgcggatga atgcccctcc ggggcgtttg aacggattgg
    15181 tcgcgatatc agccttgacg ctctggaacg ggaagtgatg aaagatgaca ttttctttcg
    15241 cacgtccggc ggcggcgtca cgctttctgg cggcgaagtg ttaatgcagg cggagtttgc
    15301 tacccgtttt ttacagcgac tgcggctgtg gggtgtctca tgcgccattg aaactgccgg
    15361 agacgcgcca gcaagcaagc tgttaccgct ggcgaaattg tgcgatgaag tgctgttcga
    15421 tttaaaaatt atggacgcga ctcaggcgcg ggatgtggtg aagatgaacc tgccacgcgt
    15481 gctggagaat ctgcgtttgc tggtgagtga gggtgtcaac gtgatcccgc gtttaccgtt
    15541 gatccctggt ttcacgctca gccgggagaa tatgcagcag gcgctggatg tactgatccc
    15601 gctgaaaatc aggcagatcc atctgttacc gtttcatcag tacggcgaac cgaaataccg
    15661 cctgctgggg aaaacatggt cgatgaaaga ggtgcctgcg ccgtcgtcag ccgatgtgac
    15721 aacgatgcgc gaaatggcag aacgggccgg atttcaggtt accgtgggag gttaaaatgg
    15781 catacctggt ggcagtaacc gcctgcgtaa gcggcgtggc gcatacttat atggcggcgg
    15841 aacggctgga aaagttgtgc cagttagaga agtggggcgt cagcattgaa actcagggcg
    15901 cgctgggaac ggagaatcgt ttagcggacg aggatatccg tcgggcggat gttgctctgt
    15961 tgattacgga tatcgagctt gccggtgccg agcgatttga acattgccgc tatgtgcaat
    16021 gcagcatcta cgcattcctg cgtgagccgc agcgggtaat gagcgcggtg cgcaaagtgc
    16081 tttctgcgcc gcagcaaacc catcttattc tggagtagtc ggtttttctg tcagttggct
    16141 gtggtactgc cggcgatatt ccgacggcga gtgttcggtg tttttacgaa acagacggca
    16201 gaagtagttg ctgtcgacaa agccgcaggc gtgcgccact tcttttacct tcaggtcata
    16261 gcctttcaat aacgtcttag cgtgctccag tcgcgtgtga ttcaggtatt cgttaaaacc
    16321 aatggccccc gttttttgaa acaggtgcga gagatagttt ggtgaaatat agaatgcctg
    16381 tgcgacagat tcgcgggtaa gcgcggaggc atagcgttcg tcgatataat cgcgaatagc
    16441 ttcaaatagt gcctggctgc gtgaggcggt ctggatttgg ctgccgagca aatcgcggca
    16501 gtggctaagc aggctggcta cgataagcct tgccgtttgc tgctcctgcg gctgcatttg
    16561 catttcattg agcgtttgta gcagaaaaga acctatgcgt gggccgcgtc gggcgacgtg
    16621 ttgcttcgcc agattttgat attgtttgcc atcccattgc acgacgctga agccgagttg
    16681 ctgtttgcca aacagcacgc taaaggtggt agcgggtgtt ttccattgtg ggaaattcca
    16741 gccaccagcg gcaacgtaca gcacatcgcc gggaactaac gttgctccag cgccggtatc
    16801 aacaaactca ccttccagca caatttccag ccgtggaaaa tcgacctgat acgcaagatc
    16861 gggcaccggg ccgttactgc tggcaaagta aatctggcgc agggacagcg ggccgttgat
    16921 caggcgggag agcagatagc tgacgtcgtg atacatgtta ttcccttagg tatgtagacg
    16981 ggaaaacagt aactggcatt attaaaaaag gccacctgaa aagtgtggcc tgaggggagt
    17041 tcgatgtaaa cattgcggca gtatcgcatc cggcaaatag cgccagacgc gatacaaagg
    17101 tcatctactg attacccacc tgatcgccat acggcagagt gtcgtaatcg atcagcgcgt
    17161 ttttcttgta cgggttacca atccagcggg tagtttcttt gaactgcgga ttcaccactg
    17221 aacgggttgg atcgtaaccg tcgtaagata agcccgccaa atctgaccag gtgtggatca
    17281 gttccgccag gctgtattta cgatcgacat cctgtgagaa atcacgggga tgagtcgctt
    17341 gccatttttc cgacgtccac agcaggaacg gaatggtgta catgtggcgc gtcgggttgt
    17401 cttcgttacg cccctgagtt ttgtgcggcg gcgtgtcgta aacctcttca ccgtggtcag
    17461 agaagtaaac caggaatccg ttcgggtctg ccgctttaaa gtctttaatc aggctggcaa
    17521 ccacatgatc gttatacagg ttagcgttgt cataatcgtt atatgattcc agctcttctg
    17581 cgcttaatcc tggcggaaca tgatcggtat tgccatcaaa cttgccctga ttttccgggt
    17641 agcggtattt gtatttgata tgcgtaccca gcagatgaac gatgatcagt ttcttcggcg
    17701 cagggtcatt cagcacatcc tggaacggct tcagcacgtt ggtgtcgtat tcacgcgcac
    17761 tctgcgtacg ttgctggttc atgtagtact gcttgtcggt ctggcgcgaa aataccgtca
    17821 gcatggtatt gcgggcggtc atcgtctgct ggttggtgat ccagaaggtt ttataacccg
    17881 cctgtttcat catgttcatc agcgacggct gcgtcagata cagatccggg ttcttttcat
    17941 tggcaaaggt cagcgcctgt tgcaggattt caatggtgta cggacgagac gtaactacgt
    18001 tattaaacac ggtcagattc ggatcggttt tatgcagcgc atccagctcc ggcgtggttt
    18061 cacgcggata accgtacaga ctcatgcgtc cgcgctgggt cgactcgcca atcaccagca
    18121 ctaaagtgcg cggttcgtta cccgattcat ctttgaaatt agccagtggc ggcaaggcat
    18181 tattttcatt cagtaacttt gttagcgagt ttagttgctg acgatactga taatagccgg
    18241 tcaggaattg ccacggtgcg gcaggctcca tacgcgaggc taggttatcc aacgttttct
    18301 caaacggctt gtttttgata aacgtattca tggcgatcgg atgcagaatc aagccataaa
    18361 gcagggcaaa agagacaaca taacgccacg gctttggaat atagaccggg cgcaggcgtg
    18421 tccacagcag aactgccacc gccgtatagg ccagcgcgat aagcacaatt ttcaggctga
    18481 aatactggct taaatactcg ctggcttcgt tggtgttggt ttcgaacatc acaaacagaa
    18541 cgctctgcga gaactcctgc ccgtagatga cgtagtagca cagcgccgcc agagaggccg
    18601 tccatagcac cacgccgatc actgcggcaa taattttaat ccgcttcgga aagaggaata
    18661 ccgggatcaa ccacagcgag ctgaataaca gcgagtcgcg aatgccgttg gtgccactat
    18721 aaccactgat gtaaataatg gcctgtagca gagtagagaa aaaccaaaag tagagcagtg
    18781 cccaacccag ggctttccag ctagaaaggg gtttagcctg gacttctgtg gaatgcatag
    18841 taagaacctg tcttgaaaaa aatatcgccg aatataacga caattcctta aggatatctg
    18901 aaggtatatt cagaatttga ataaaatgca gacagaaata tattgaaaac gagggtgtta
    18961 gaacagaagt atttcagaaa accctcgcgc aaaagcacga gggtttgcag aagaggaaga
    19021 ttagccggta ttacgcatac ctgccgcaat cccggcaata gtgaccatta acgcttgttc
    19081 gacgcgagga tccggttcct ggccttcttt ttctgcctgg cgggagcggt gcagcaactc
    19141 ggcctgcaat acgttcagcg ggtcggtgta aatattccgt agctgaatag actctgcaat
    19201 ccacggcaga tcggccatca gatgggaatc gttggcaatc gccagcacca ctttgatgtc
    19261 ttcttcttgc aggttgcgta actctttacc taacggccac agtgctttgt ctaccagacg
    19321 ttgatcatag tattccgcca accacaggtc tgctttggcg aagaccatct ccagcatgcc
    19381 gagacgcgtc gagaagaatg gccaatcgcg gcacatggct tccagctcgc tctgtttgcc
    19441 atcttcgacc actttttgca gcgccgtacc tgcacccagc caggcgggga gcatcaggcg
    19501 gttttgcgtc caggcaaaaa tccacggtat ggcgcgcagc gactcgacgc cgccggttgg
    19561 gcgacgtttc gccggacgtg aacccaacgg cagtttgccc aattcttgtt ccggcgtagc
    19621 ggagcggaag taaggcacaa aatctttgtt ttcacgtacg tagccacggt agagatcgca
    19681 ggagatgact gacagttcat ccataatgcg acgccagctc tctttcggct caggtggtgg
    19741 cagcaggttg gcttccagaa tcgccccggt gtaaagcgac aggctgctga cggtgatttc
    19801 tggcagacca tatttaaagc ggatcatctc gccctgttca gtcacgcgca ggccaccttt
    19861 caggcttcct ggcggttgtg acagcagcgc cgcatgggca ggtgcgccgc cgcgaccaat
    19921 ggaaccgccg cgaccgtgga acaacgtcag ctcaataccc gctttttcgc aggttttgat
    19981 taatgcatcc tgtgcctgat attgcgccca ggaagctgcc atcacgcccg catcttttgc
    20041 tgagtcggaa tagccaatca tcaccatctg tttgccctga atcaggccgc gataccagtc
    20101 gatattgagc agctgggtca tgacatcgtt ggcgttgttc aggtcatcga gggtttcaaa
    20161 cagcggagca accggcatcg caaacccgat acccgcttct ttcagcagca ggtggacagc
    20221 cagtacgtcg gacggcgttt tcgccatcga gatcacgtag gcggcaatgg agccttgcgg
    20281 cgcttcggca atcacctggc aggtatcgag cacttcgcgc gtttcggcgc ttggttgcca
    20341 gttgcgcggc agaagcggac gtttggagtt cagttcgcgg atcaggaacg cctgtttgtc
    20401 ggcctctgac cagctttcgt agtcgccgat accgaggtag cgggtcagct cgcccagcgc
    20461 ttcggtatgg cgggtgctct cctgacggat atcaatacgg accagcggta cgccgaaaca
    20521 tttcacgcgg cgcagggtgt cgagcagatc gccgttggcg ataataccca tgccacacgc
    20581 ctgaagtgac tggtagcaag cgtagagcgg ttcccacagt tcttcgtttt gtgtcagcag
    20641 cccttctggt tttggcagtt cttcgccttt caggcgcgct tccagccatg cctgtgtcgc
    20701 catcaggcga gaacgcaggt ttttcatcag atagcgatac ggttctgcgg caccttcttc
    20761 gccaaccagc gccagcagtt caggggtcgc ttcaaccatc gacagttcag aaaccagaac
    20821 ctgaatatct ttcaggaaca gatcggtggc tttccagcgg ctgagcagca ggacgtggcg
    20881 ggtgatatcg gcagtgacgt tcgggttgcc gtcgcggtca ccgcccatcc acgaggtaaa
    20941 acgtaccgga acaaattcga cgggcagttt gtagccgaga ttctcttcca gttgttcgtt
    21001 cagttcgcgc aggtaattcg gtacgccttg ccacaggctg ttttctacta cggcaaagcc
    21061 ccatttggct tcatctaccg ggcttggacg cagcttacgg atttcatcgg tatgccatga
    21121 ctgggcgatc aactggcgca ggcgacgcat cagctggttg cgttcgtagt cagcgatatc
    21181 tttgttatcg agctgtttta aacaggcgtt cacttccacc attttgtgga tcagtgtacg
    21241 acgggtaatt tcggttgggt gagccgtgag gaccagttcc agcgacagcg attccactgc
    21301 ttttttgatg gtgtcttcgc tcagttccgg ctggtttttc agtttacgca gggtgcgggc
    21361 gatcacttcc gggttgctgg cagcttcgcc tttcggcgaa atgctgtggt attgctcggc
    21421 ggtgttggcc aggttcagga actgactaaa cgcacgcgca acgggcagca gctcgtcgtt
    21481 cgacaaattt tgtaaggtgg tgagcaactc ctggcggtta gcatcattgc cagcgcgtga
    21541 agatttcgac aacttacgga tagtttctac gcgttcaaga atgtgttctc ccaacgcatc
    21601 cttgatggtt tctcccagca ctttgccgag catactgaca ttactacgca atgcggaata
    21661 ttgttcgttc atattacccc agacacccca tcttatcgtt tgatagccct gtatccttca
    21721 cgtcgcattg gcgcgaatat gctcgggctt tgcttttcgt cgtcttttat aaagccacgt
    21781 aaaagcggtg acgtcaaatg ctgcgaaatc gcttcagcaa acgaataaat agcaggaatt
    21841 tacgccatta aattcacgac gctttaaata agcgtaactt atggaaatgt taaaaaatcg
    21901 ccatacataa caccaaaggt gtaggtcgga taagatgcgc aagcatcgca tccgacatta
    21961 ttgcggcacc ggagtttggc aacggtgccg gatgcggcgc gagcgcctta tccggcctac
    22021 gttttaatgc cagcaaaaat ggtgaattac ctggataatc aactcacggg tgggtttaat
    22081 aaaccgcgtc tccagatatt catcaggctg atgagcttga ttgatggaac ccggaccgag
    22141 caccagcgtc gggcataacg tttgaataaa cggcgcttcg gtacagtaat tcaccacttc
    22201 ggtttttgct ccgagcaatt tctcaaccac ttcaaccagt tgatgattcg gtgggcattc
    22261 atagccaggg atcggcggat gcagctcgtc gaccgtcaga cgacccggcc agcgttcgct
    22321 caccggagcc aatgcatcgt tgagcaaacc attaagttca ttgagtgtca tgccaggctg
    22381 cggacgaata tccatatgca actcacagca agcgcaaata cggttagaag cgtcgccacc
    22441 gtgaatatgc ccgaggttga gcgtagggta tggcacggta aatgcttcgt agtgataacg
    22501 ttctttcagg ttatcgcgca attgcaaaat atgcccgatg gcgtcgtgca ttagttcgat
    22561 agcgttaatt ccgcgtgctg gatcgctgga gtgccccgac tggccctgaa tacggatggc
    22621 gttagagata tgacctttat gtgcgcgtac cggttgtagt gacgtcggtt cgccaatgat
    22681 ggcgcaatcc gggcgcaggg cggtagtttc ggcaaaataa cgcgctccgg ccatactggt
    22741 ttcttcatca gcagtcgcca gaatgtagag cggttttttc agtttcgtga cgtcgacatc
    22801 gcgtagcgca tcaaggataa acgcaaaaaa gcctttcatg tcggcggtgc ctaagccgta
    22861 aagcttgccg tcatgctccg tcagtgtaaa cggatcgcgc gtccagcgac cgtcatcaaa
    22921 tggcaccgta tcggtatgcc ccgccagcaa caagccgcca gccccctgtc cgtaactggc
    22981 cagcatatta aatttgttgc gagttcctgg aacaggctgc acttccacat tgaagcccaa
    23041 atctttaaac cagtccgcca gcagagtgat taaatctgca ttgctttgat cgagtgcctc
    23101 ttccgtagcg cttattgaag gtgtggcaat cagagcgcgg taaatctcga taaatggcgg
    23161 taatttgttt ttcattgttg acacacctct ggtcatgata gtatcaatat taatgcagta
    23221 tttatgaata aaaatacact aacgttgagc gtaataaaac ccaccagccg taaggtgaat
    23281 gttttacgtt taacctggca accagacata agaaggtgaa tagccccgat gttgaatacg
    23341 ctgattgtgg gtgccagcgg ctacgctggc gcagagctag tgacctatgt aaatcgccat
    23401 ccgcatatga acataaccgc tttgactgtt tcagcgcaaa gcaatgatgc gggaaagtta
    23461 atctccgatt tgcatccgca gctaaaaggc atcgttgagc tgccgttgca gccgatgtcg
    23521 gatatcagcg agtttagccc aggggtggac gtagtgtttc tcgccaccgc ccatgaagtt
    23581 agccacgatt tagcgccgca gtttcttgaa gcgggctgcg tggtgttcga cctttccggc
    23641 gcgtttcgtg ttaacgacgc caccttctat gaaaaatatt acggctttac ccatcaatac
    23701 ccggaactgc tggaacaggc agcctacggt ctggcggagt ggtgcggtaa taaattaaaa
    23761 gaagcgaatt tgattgcggt gccaggctgt tatccgacgg cggcacagct ggcgctgaaa
    23821 ccgttgattg atgccgatct tcttgacctc aatcagtggc cggtgatcaa cgccaccagc
    23881 ggcgtgagcg gtgcaggacg taaggcagcg atttcaaata gtttttgtga agttagcctg
    23941 caaccgtatg gcgtctttac tcatcgccat caaccagaga tcgccacaca cctcggtgct
    24001 gacgttatct tcaccccaca tctgggcaat ttcccgcgcg gcattctcga aaccattacc
    24061 tgccgcctga aatcgggtgt gacccaggcg caagtcgcgc aagcgttaca gcaggcgtat
    24121 gcccataaac cgctggtgcg gctgtatgac aaaggcgttc cggcgctgaa aaatgtcgtt
    24181 gggctgccat tttgcgatat cgggtttgcc gttcagggcg agcatctgat tattgtggcg
    24241 accgaagaca acttactgaa aggcgcggcg gcacaagcgg tacagtgcgc caatattcgt
    24301 ttcggctatg cggaaacgca gtctcttatt taagggtgca atgatgaatc cattaattat
    24361 caaactgggc ggcgtactgc tggatagtga agaggcgctg gaacgtctgt ttagcgcatt
    24421 ggtgaattat cgtgagtcac atcagcgtcc gctggtgatt gtgcacggcg gcggttgcgt
    24481 ggtggatgag ctgatgaaag ggctgaatct gccggtgaaa aagaaaaacg gcctgcgggt
    24541 gacgcctgct gatcagatag acattatcac cggagcactg gcgggaacgg caaataaaac
    24601 cctgttggca tgggcgaaga aacatcagat tgcggccgta ggtttgtttc tcggtgacgg
    24661 cgacagcgtc aaagtgaccc agcttgatga agagttaggt catgttggac tggcgcagcc
    24721 aggttcgcct aagcttatca actccttgct ggagaacggt tatctgccgg tggtcagctc
    24781 cattggcgta acagacgaag ggcaactgat gaacgtcaat gccgaccagg cggcaacggc
    24841 gctggcggca acgctgggcg cggatctgat tttgctctcc gacgtcagcg gcattctcga
    24901 cggcaaaggg caacgcattg ccgaaatgac cgccgcgaaa gcagaacaac tgattgagca
    24961 gggcattatt actgacggca tgatagtgaa agtgaacgcg gcgctggatg cggcccgcac
    25021 gctgggccgt ccggtagata tcgcttcttg gcgttatgcg gagcagcttc cggcactgtt
    25081 taacggtatg ccgatgggta cgcggatttt agcttaagtt ttgttagccg gaggcgcagc
    25141 tttccggcat tgaatttcaa aataaggaaa cagagttatg gcactttggg gcgggcgttt
    25201 tacccaggca gcagatcaac ggttcaaaca attcaacgac tcactgcgct ttgattaccg
    25261 tctggcggag caggatattg ttggctctgt ggcctggtcc aaagccttgg taacggtcgg
    25321 cgtgttaacc gcagaagagc aggcgcaact ggaagaggcg ctgaacgtgt tgctggaaga
    25381 tgttcgcgcc aggccacaac aaatccttga aagcgacgcc gaagatatcc atagctgggt
    25441 ggaaggcaaa ctgatcgaca aagtgggcca gttaggcaaa aagctgcata ccgggcgtag
    25501 ccgtaatgat caggtagcga ctgacctgaa actgtggtgc aaagataccg ttagcgagtt
    25561 actgacggct aaccggcagc tgcaatcggc gctggtggaa accgcacaaa acaatcagga
    25621 cgcggtaatg ccaggttaca ctcacctgca acgcgcccag ccggtgacgt tcgcgcactg
    25681 gtgcctggcc tatgttgaga tgctggcgcg tgatgaaagc cgtttgcagg atgcgcttaa
    25741 gcgtctggat gtcagcccat taggctgtgg cgcgctggcg ggaacggcct atgaaatcga
    25801 ccgtgaacag ttagcaggct ggctgggctt tgcttcggcg acccgtaaca gtctggacag
    25861 cgtttctgac cgtgaccatg tgttggaact gctttctgct gccgctatcg gcatggtgca
    25921 tctgtcgcgt tttgctgaag atctgatttt ctttaacacc ggcgaagcgg ggtttgtgga
    25981 gctttcggac cgcgtgactt ccggttcatc attaatgccg cagaagaaaa acccggatgc
    26041 gctggagctg attcgcggta aatgcggccg ggtgcagggt gcgttaaccg gcatgatgat
    26101 gacgctgaaa ggtttgccgc tggcttacaa caaagatatg caggaagaca aagaaggtct
    26161 gttcgacgcg ctcgatacct ggctggactg cctgcatatg gcggcgctgg tactggacgg
    26221 cattcaggtg aaacgtccgc gttgtcagga agcggcgcag caggggtatg cgaacgccac
    26281 tgaactggcg gattacctgg tggcgaaagg cgtacctttc cgcgaggcgc accatattgt
    26341 gggtgaagcg gtggtggaag ccattcgtca gggcaaaccg ctggaagatc tgccactcag
    26401 tgagttgcag aaattcagtc aggtgattgg cgaagatgtc tatccgattc tgtcgctaca
    26461 atcgtgcctc gaaaagcgtg cggcaaaagg cggcgtctca ccgcagcagg tggcgcaggc
    26521 gattgctttt gcgcaggctc ggttagagta atttgtttat attgctcccc tgaaaatggg
    26581 gagtttacca cgcctacaaa gtgcgacact tcgtaggcgg ataaggcgtt tacgccgcat
    26641 caggcagaaa tttgtcagca ttc
//
LOCUS       DADLTY010000041        26561 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.041, whole genome shotgun sequence.
ACCESSION   DADLTY010000041 DADLTY010000000
VERSION     DADLTY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.041"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            346..639
                     /locus_tag="JN334_001461"
     CDS             346..639
                     /locus_tag="JN334_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437648.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            711..1370
                     /locus_tag="JN334_001462"
     CDS             711..1370
                     /locus_tag="JN334_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAY7437649.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1447..1908
                     /locus_tag="JN334_001463"
     CDS             1447..1908
                     /locus_tag="JN334_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HAY7437650.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKDLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2115..3015)
                     /gene="hlyE"
                     /locus_tag="JN334_001464"
                     /pseudo
     CDS             complement(2115..3015)
                     /gene="hlyE"
                     /locus_tag="JN334_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3388..3807
                     /gene="umuD"
                     /locus_tag="JN334_001465"
     CDS             3388..3807
                     /gene="umuD"
                     /locus_tag="JN334_001465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY7437651.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3807..5075
                     /locus_tag="JN334_001466"
     CDS             3807..5075
                     /locus_tag="JN334_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAY7437652.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5121..5651)
                     /gene="dsbB"
                     /locus_tag="JN334_001467"
     CDS             complement(5121..5651)
                     /gene="dsbB"
                     /locus_tag="JN334_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HAY7437653.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5797..7338)
                     /gene="nhaB"
                     /locus_tag="JN334_001468"
     CDS             complement(5797..7338)
                     /gene="nhaB"
                     /locus_tag="JN334_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HAY7437654.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7559..8278
                     /gene="fadR"
                     /locus_tag="JN334_001469"
     CDS             7559..8278
                     /gene="fadR"
                     /locus_tag="JN334_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HAY7437655.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8330..9862)
                     /locus_tag="JN334_001470"
     CDS             complement(8330..9862)
                     /locus_tag="JN334_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAY7437656.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10192..11490
                     /locus_tag="JN334_001471"
     CDS             10192..11490
                     /locus_tag="JN334_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HAY7437657.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11500..12570
                     /gene="dadX"
                     /locus_tag="JN334_001472"
     CDS             11500..12570
                     /gene="dadX"
                     /locus_tag="JN334_001472"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HAY7437658.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12738..14152)
                     /locus_tag="JN334_001473"
                     /pseudo
     CDS             complement(12738..14152)
                     /locus_tag="JN334_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109184.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14763..16499)
                     /locus_tag="JN334_001474"
     CDS             complement(14763..16499)
                     /locus_tag="JN334_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAY7437659.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16594..17508)
                     /gene="ldcA"
                     /locus_tag="JN334_001475"
     CDS             complement(16594..17508)
                     /gene="ldcA"
                     /locus_tag="JN334_001475"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HAY7437660.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17608..18219
                     /gene="emtA"
                     /locus_tag="JN334_001476"
     CDS             17608..18219
                     /gene="emtA"
                     /locus_tag="JN334_001476"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HAY7437661.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18221..18955)
                     /locus_tag="JN334_001477"
     CDS             complement(18221..18955)
                     /locus_tag="JN334_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HAY7437662.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19156..19410
                     /locus_tag="JN334_001478"
     CDS             19156..19410
                     /locus_tag="JN334_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY7437663.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19460..21414)
                     /locus_tag="JN334_001479"
                     /pseudo
     CDS             complement(19460..21414)
                     /locus_tag="JN334_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(21440..22294)
                     /locus_tag="JN334_001480"
     CDS             complement(21440..22294)
                     /locus_tag="JN334_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HAY7437664.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22294..23103)
                     /locus_tag="JN334_001481"
     CDS             complement(22294..23103)
                     /locus_tag="JN334_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAY7437665.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23113..23871)
                     /locus_tag="JN334_001482"
     CDS             complement(23113..23871)
                     /locus_tag="JN334_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7437666.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23868..24848)
                     /locus_tag="JN334_001483"
     CDS             complement(23868..24848)
                     /locus_tag="JN334_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAY7437667.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24848..25870)
                     /locus_tag="JN334_001484"
     CDS             complement(24848..25870)
                     /locus_tag="JN334_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HAY7437668.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26220..>26561
                     /locus_tag="JN334_001485"
     CDS             26220..>26561
                     /locus_tag="JN334_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437669.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT         6673 a         6470 c         6855 g         6563 t
ORIGIN      
        1 tggcctttgc tgaactcaac attgacattc ttacccgcag caaagctcgt ggaggttagc
       61 agtgcaagca gcaatacagc cttactgatg ctcttgattt tcattggtag tctcctgttc
      121 agaatgagta cgatgaactg ttataatata acaatccctt acgggagaat tccagtggct
      181 tttctgcgtt cggtaaaatc ccggcgcggc attttttgcc ggggggatta cttataaagg
      241 ggcatggact caacaaaccg cagaaatcta cagctggctg gcttgtggcg cgggtttcat
      301 tgatgttagc ttatgccaaa acctggcatt ttaaagagta aaagtatgtt ttgtgcgatt
      361 tatcgaagca gcaagcgtga ccagacctat ttatatgtcg aaaaaaaaga cgatttttcg
      421 cgtgttcctg aggaactgat gaaaggtttt ggtcagcctc agttagcgat gattctgccg
      481 ttggatgggc ggaagaaact ggtcaatgct gatattgaaa aggttaaact ggcattaacc
      541 gagcaaggtt actatttgca gttaccgcca ccacccgaag atttgctgaa gcaacatctt
      601 tccgtcatgg ggcagaaaac agacgacact aacaaataac cgatatccgg cggtggcatt
      661 atctttgtcg gcgcgggttt tcatatccac gataaggtga ggggaacgtt atgtatcaac
      721 atcacaactg gcaaggtgcg ctgctggatt atccggtgag taaagtagtc tgtgttggca
      781 gtaactatgc caaacatatt aaagagatgg gcagcgcagt gcccgaagag ccagtgctgt
      841 ttattaaacc agaaacggca ctgtgcgatc tgcggcagcc attggcgatc ccatccgatt
      901 tcggttcagt tcatcatgaa gtcgaactgg cggtgttgat tggcgcgacg ctgcgtcagg
      961 caacggaaga gcatgtccgc aaagccattg ccggttacgg cgtggcgctt gatctgacat
     1021 tgcgtgatgt tcagggaaaa atgaagaaag ccgggcagcc gtgggaaaag gctaaagcgt
     1081 ttgataactc ttgtccgctt tccgggttta tccccgcggc ggaatttacc ggcgatccac
     1141 aaaatacaac gctgggcctg agcgtaaacg gcgaaaaacg ccagcaaggt acgactgcgg
     1201 acatgatcca taaaatcgtt ccgttgatcg cttatatgag caagtttttt accctcaagg
     1261 ccggtgacgt tgtgttgaca ggcacgcctg atggcgtcgg cccgttgcaa agcggtgatg
     1321 agctgacagt cactttcgat gggcattctt tgacaactcg cgttttgtaa tactttttgc
     1381 cgccagaaag cggcggcaaa acttgcatcg ctgtgccaga ctggttataa ggtgcgtttt
     1441 aacgtaatgg cggaacacct gatgagcgat gtacctttct ggcaaagtaa aaccctggac
     1501 gaaatgagcg atgcggaatg ggagtcgttg tgtgatggtt gcggtcagtg ttgcttgcat
     1561 aaactgatgg atgaagacac cgacgaaatc tacttcacta acgtcgcctg tcgccagctc
     1621 aatattaaaa cctgtcaatg tcggaactac gaacgtcgtt tcgagtttga acccgactgc
     1681 attaaattaa cccgtgaaaa tctgccaaca ttcgaatggc tgccaatgac ctgcgcttat
     1741 cggttgctgg cggaaggtaa agatttacct gcgtggcatc cgctacttac tggttcgaaa
     1801 gcggcaatgc atggtgaacg tatctctgtg cgtcatatcg cagtgaaaga atcagaagtg
     1861 attgactggc aggatcatat cttaaataaa cctgactggg cgcagtgata aatttaatag
     1921 atgctgatgg gggctttctt aatatacgtc tggatgctcg ccatttcgta gccctgtttt
     1981 tgaaagcgct ctgtcataat gagagttacc cgatacctta ttatcaacag gtaactctca
     2041 atctttaaaa aacgtctgtg tttttaaatg tgataaacct tataccttga gtacatggag
     2101 agaataatcg cttatcagac ttcaggtact tcaaagagtg tctttttacc gtgtcttttc
     2161 tgatactcat tacaggtgtt aatcattttt ttggccgctg cttttagcaa agaaagcatt
     2221 aaatcatcat aatcaacata gaatctggtt gtttcagttt ccgtttttat ctccccgatg
     2281 gcggctattt cggtggttaa tttcaatttg gcggcatcga tatctttatt cgcttgttta
     2341 accgtgttag acagggtggt aaagaaactc tgcacagatt ttaacttgtt cttcaattct
     2401 ggaatcagtt tcccttcaac tacgcccgca gcaatagaat aggaaatgat taaaccaaat
     2461 ggaccggcga cgacatcggc tgcggcaccg gcatacgctt ccttcctgat tttatctacc
     2521 tgtgactgga aatagctgct tttttctgaa aaatcattgg ttaactggct atctaacgcc
     2581 agcagtttcc cggaagcgtt gttgaaactt tgtgagctta ccagcaggga tttttgcgct
     2641 tcattcagct tcgtgatgcc gtcatccagt accttaatga ggggcggatg ctttcttctc
     2701 attgtactca tcaaatagca aaatatacgc tgcgagcaat tgcgtcgcaa caccacacca
     2761 ttcatacact gtttgggttg cttcaaaata cttatcctgg ctatccataa gtaaggtttt
     2821 aatatcgccg actaaaacgg aggctgcctg tgaatactcc tgtttaaagc gacttaactc
     2881 ttttatggtt tcatcaaagg tctgccaggg gatgacctga tcgagatatt tattataaag
     2941 atctaatgct ccatctgcgg tttcgattgc gtttttaact acttctaccg ttttatctgc
     3001 aacgatttca gtcataatca ttcgcctctt taaatatata aattgtaatg aaactcctgt
     3061 tttacaacta ttaataaatt ttacttcatc taattcatag ttagccgggc gggatgcgtc
     3121 aatgtcttta tttctattaa tatgataaat atcaaacaat gtttaatgtc attatggcga
     3181 atgcttctat tctatttttt tgccgggtga tatttttcat ttctgctgga tgagcgtcgt
     3241 cgccagaagg ccacgtgagc acaggataag agaacgaaaa atcagcagtc tatgcagcga
     3301 caaatattga tagcctgaat cagtattgat ctgctggcaa gaacagacca ctgtatataa
     3361 aaacagtata acttcaggca gattattatg ttgtttatca agcctgcgga tctccgcgaa
     3421 attgtgactt ttccgctatt tagcgatctt gttcagtgtg gctttccttc accggcagca
     3481 gattacgttg aacagcgcat cgatctgaat caactgttga tccagcatcc cagcgcgact
     3541 tacttcgtca aagcaagtgg tgattctatg attgatggtg gaattagtga cggtgattta
     3601 ctgattgtcg atagtgctat taccgccagc catggtgata ttgtcatcgc tgctgttgac
     3661 ggcgagttta cggtgaaaaa attgcaacta cgcccgacgg tacagcttat tcccatgaac
     3721 agtgcgtact cgcccattac catcagtagc gaagatacgc tggatgtctt tggtgtggtg
     3781 atccacgtcg ttaaggcgat gcgctgatgt ttgccctctg tgatgtaaac gcgttttatg
     3841 ccagctgtga gacggtgttt cgccctgatt tatggggtaa accggtggtt gtgctatcga
     3901 ataatgacgg ttgcgttatc gcccgaaacg ctgaggcaaa ggcgctgggc gttaaaatgg
     3961 gcgatccctg gttcaaacaa aaagatctgt ttcgtcgctg tggtgtggtt tgctttagca
     4021 gcaattatga gctttacgca gacatgagca atcgggtgat gtcgacgctg gaagaactat
     4081 cgccccgcgt cgagatttac agtattgatg aagcattctg cgatctgaca ggtgtgcgta
     4141 attgtcgcga tctgactgat tttggcagag aaattcgcgc aacggtgcta caacgtaccc
     4201 atcttactgt tggtgtgggg atcgcccaga ccaaaacgtt ggctaagctt gccaatcatg
     4261 cggcaaaaaa atggcagcgg cagacgggtg gggtggtgga tttatcaaat ctggaacgcc
     4321 agcgtaaatt aatgtctgct ctccccgtgg atgacgtctg ggggattgga cggcggatca
     4381 gcaaaaaact ggacgcgatg gggatcaaaa ccgttctcga tttggcggat acagatatcc
     4441 ggtttatccg taaacatttt aatgtcatgc tcgaaagaac ggtgcgtgaa ctgcgcggcg
     4501 aaccctgttt gcaactggaa gagtttgcac cgacgaagca ggaaattatc tgttcccgct
     4561 cgtttggtga acgcatcacg gattatacgt cgatgcggca ggccatttgt agttacgctg
     4621 cccgggcggc ggaaaaactt cgcagtgagc atcaatattg tcggtttatc tccacattta
     4681 ttaagacgtc accatttgcg ctcaatgaac cttattacgg caatagcgcg tcggtaaaac
     4741 tgctgacgcc cactcaggac agcagggata tcattaacgc tgctacgcga tctctggatg
     4801 ccatctggca agcgggccat cgctaccaaa aagcgggcgt gatgctgggg gatttcttca
     4861 gtcagggagt cgcgcagctc aatttattcg atgacaacgc accgcgcccc gggagtgagc
     4921 aattgatggc ggtaatggat actctgaatg ctaaagaggg cagaggaaca ctctattttg
     4981 ccgggcaggg gatccaacaa caatggcaga tgaagcgagc tatgctttca ccacgttata
     5041 caacgcgaag ttctgattta ctgcgggtca aataaatata gcggcaggaa aaaagcgctc
     5101 ccgcaggagc gccgaatgga ttagcgaccg aacagatcac gttttttcgc tttaaacggc
     5161 tgggaaatca ccaccagcac cgcgacaatc aggtaagcga taaaaatacc gagcagccac
     5221 tgcggcattt ccatacctaa aaaatcccac tgacgctcgg cgcaatcgcc agaggcgaca
     5281 aacacttgcg gcacccattt atccagcggc agccattccg ggaaacgaac cataaaatca
     5341 caggtggcaa atggcgaagg atagagctga agcatggtgt gctcgtaagt taactgcaca
     5401 ccgcggaacg cactatacaa ccagataacc atcgctacat aacgcagcgg agttttcggg
     5461 gcgatcgcgc caatcagcgc agcacccaga acgccgaata acgcgcagcg ttcataaata
     5521 cagagcacgc aaggtttcag taacatcaca tgctggaacc acagcgccgt cagttccagt
     5581 gccagagcag taaacgccat caacagccac gcgccccggc cttgtgaaca ttggttcaaa
     5641 aatcgcaaca taatcatttc cctgcaatat gcatagagtg cgcagtttaa accaattcat
     5701 tcgctgcgcc accaggaggc agcaaaaata ggacgcaatt ggacaattat cgggcgaaaa
     5761 ggcaaaccgg gcatttatat gcccggtaag ttgttatcaa agcgttgcta tccagcccat
     5821 ttgcataaac cattcggtta caggggcaag cgtaaactcg acgcagagca agccgacgag
     5881 tgtcagtacg agggtgtaag gcagggccat ccacaccatg cggccataag agaggcgaat
     5941 caatggcgcg agtgcagagg tcagcaggaa caggaacgca gcctgaccgt tcggcgtagc
     6001 gacagagggc agattggtac cggtattaat ggcgaccgcc agcagctcgt attgcttcaa
     6061 cgtgatagcg ccactttcca ttgccgcttt cgcttcgttg atataaatcg tccccacgaa
     6121 gacgttatcc gaaatggatg acagcagacc gttgaaaata tagaacagcg acagctgagc
     6181 atgttcagat gcctgcaaca caaactgaat aatcggcgaa aacagttgtt ggtcgataat
     6241 caccgcgact accgagaaaa acaccgtcaa cagtgcggtg aatggcagag attcggtgaa
     6301 ggctttaccg atagcatgtt catcggtgac accggtcaat gatgttgcca gaataatgac
     6361 tgacaaacca atcaagccaa cttccgccag atgcaacgcc agcgcagtca ccagccagac
     6421 gccaataatc gcctggacaa tcagacggat tttatcctga cgggtacgct ggtggcggct
     6481 ttgatcgtca aactgttgca acacttcgcg gactttctcc ggcagcgttt caccgtagcc
     6541 aaaccaacgc agcttctcta ccagcaggca ggttaacagg ccacaaatca gaaccggaac
     6601 ggtcaccggc gacatgcgca ggaagaaatc gccaaaatgc cagccagccg ctttagcgat
     6661 gatcaggttc tgtggttcgc ccaccatggt cattacgccg cctaatgcgg tgccgacacc
     6721 ggcatgcatc atcaggctac gcagaaagcc acggaactgt tccagaacca cgttgtaatg
     6781 cttgtcgata tgactatcgt cttgcaggtc ggtgtcttcg gtacgggaag aggctacgcg
     6841 atgataaata ccataaaaac cgactgcaac gctgatcacc accgccacga cggttaaggc
     6901 atcgaggaac gcggagagga acgctgccgc cacgcaaaaa gagagcgaca gcagcatttt
     6961 ggagcgaatg cttaacagca aacgggtaaa tatgaacagc aacagctgtt tcataaaata
     7021 gatacccgcc accataaaca tcaataacag caagacttca agatttgccg ccacctcttc
     7081 acggacgtgt tccgcgctgg tcatgccgat gaataccgct tcgatagcca acagaccacc
     7141 ggggagtagc gggtagcatt tcagggccat cgccagagtg aaaataaatt ccgctaccag
     7201 caaccagccc gcgacgaaag ggctgatgag gaaaattaac gggtttacga ttaagaaaat
     7261 gatgagggcg agtttgtacc agtcggggga ctggcccaaa aaattgcgcc atagcgcgcg
     7321 gccccaggag atctccatga tggtttccct taccttacaa ataatcaatg atgtttttat
     7381 gtttaaacgc aaagcttaac ggtcaggcag gagtgaggca agtcttgata gtcaagggga
     7441 aagagatgcg gaaaatgaag ccttgatccc tttttcttct ttttgtctgc tatcagcgta
     7501 gttagccctc tggtatgatg agtccaactt tgttttgctg tgttatggaa atctcactat
     7561 ggtcattaag gcgcaaagcc cggcgggttt cgcggaagag tacattattg aaagtatctg
     7621 gaataaccgc ttccctcccg ggactatttt gcccgcagaa cgtgaacttt cagaattaat
     7681 tggcgtaacg cgtactacgt tacgtgaagt gttacagcgt ctggcacgag atggctggtt
     7741 gaccattcaa catggcaagc cgacgaaggt gaataatttc tgggaaactt ccggtttaaa
     7801 tatccttgaa acactggcgc gactggatca cgaaagtgtg ccgcagctta ttgataattt
     7861 gctgtcggtg cgtaccaata tttccactat ttttattcgc accgcgtttc gtcagcatcc
     7921 cgataaagcg caggaagtgc tggctaccgc taatgaagtg gccgatcacg ccgatgcctt
     7981 tgccgagctg gattacaaca tattccgcgg cctggcgttt gcttccggca acccgattta
     8041 cggtctgatc cttaacggga tgaaagggct gtatacgcgc attggtcgtc actatttcgc
     8101 caatccggaa gcgcgcagtc tggcgctggg cttctaccac aaactgtcgg cgttgtgcag
     8161 tgaaggcgcg cacgatcagg tgtacgaaac agtgcgtcgc tatgggcatg agagtggcga
     8221 gatttggcac cggatgcaga aaaatctgcc gggtgattta gccattcagg ggcgataatc
     8281 ccttccgttt aaagagcaac cccctcaaac gaggggtttt ttgttgtttt tacagatttc
     8341 ccattcttgg cgggcaacgt tccagcaact cgatgctgcc gtcttcgttt tgctgttcga
     8401 gcatcacatc aaatccccac aggcgatgca catgcttcag gacttctttg cgcccccgat
     8461 ccagcggtgc gcgattatgt ggaatataac gcagcgtcag cgaacggtcg ccgcgcaaat
     8521 ccacgttcca gatctgaata ttcggctcca gattacttaa gttatattgc gacgataacc
     8581 ggttacggat ctcccgataa ccttcttcat tatgaatagc ggaaatctcc agataattat
     8641 gccgatcatc atccagcacg gtgaagaagc ggaaatcacg catcactttc ggtgacagaa
     8701 actggctgat aaaactctca tctttgaaat cacgcatcgc gaaatgcagc gtttccagcc
     8761 agtcggaacc ggcgatatcc gggaaccagt atttgtcttc ttccgttggc gactgacaaa
     8821 tccgtttaat atcctggaac atggcgaacc cgagggcata cgggttgatg ccgctgtacc
     8881 acgggctgtt ataggggggc tggaagacca cattggtgtg gctgtgcaaa aactccagca
     8941 taaaacgttc cgttactttc ccttcatcat acagatggtt aaggatggta tagtgccaga
     9001 aggtcgcaca gccttcgttc atcacctgag tctgtttttg cggataaaaa tactggctca
     9061 ccttacgcac aatacgcagg atttcacgct gccatgattc cagcagtggc gcatttttct
     9121 ccataaaata gagcaggttt tcttgtggtt cggacggata gcggcgcgct tcagcaaccg
     9181 ttttctcttc ctcgcgcttc ggcagggtac gccagagcat attgacctga ctttgtagat
     9241 actcttcgcg acttttctgc cgggctttct cttcttgcag cgagattttt tgcgggcgtt
     9301 tgtaccggtc cacgccgtag ttcatcagcg cgtggcacga gtccagaagc cgttctactt
     9361 catcaacgcc ataacgttct tcgcactcgg taatatattt acgggcaaaa atcagataat
     9421 cgacaatcga actggcgtcg gtccaactgc ggaataagca attgttttta aagaaagagt
     9481 tatgtccata gcaggcgtgt gccatcacca gcgcctgcat agtaatggtg ttctcttcca
     9541 tcaagtaagc gatacacggg ttagagttaa tgacgatttc ataggccagc ccttgctgac
     9601 cgtgcttata cagccgttca gtctcgataa actttttacc gaatgaccag tgcggatagt
     9661 taattggcat gccgacgctg gaataggcat ccatcatctg ttctgaggtt atcacttcaa
     9721 tctggtgcgg gtaggtatcc agccggtaga gtttcgccac ccggtctatc tctgccagat
     9781 aaacatccag caggtcgaac gtccagtcgg gtccatcgct caaacgtgtg gtgtccttat
     9841 tcatagaatc gatcgtcgcc atacgcgcac ctcattgttg tcggcgctct ctgtgtggag
     9901 cacctcattt caagcataga acacctgtta aaaaccgcgt cgccggagaa tttttttctt
     9961 tgcgatttct tattatcaga gtgccactaa tccgcttctg aacggaattt tatgctggat
    10021 aaaaagggcg ttcagcagga gatactaaag acgccatatt gccgcagagt cagggagatg
    10081 tgagtcagct caccataaaa aagccgtatg ttgaataata ttttcaactg agttatcaag
    10141 atgtaattag attattattc ttttactgta tctaccgtta tcggagtggc tatgcgagtt
    10201 gtcatactgg gaagtggtgt ggtaggcgtt gccagcgcct ggtacttaaa tcaggcagga
    10261 catgaggtca ccgtcattga tcgggagccg ggggccgccc tggaaaccag tgccgcgaat
    10321 gccgggcaaa tctcccccgg atatgctgca ccgtgggcag caccaggtgt gcctttaaaa
    10381 gcgattaaat ggatgttcca gcgccatgcg ccgctggcgg ttcgtctcga cggtacgcag
    10441 tttcagttga aatggatgtg gcaaatgtta cgtaactgcg acaccagcca ctacatggaa
    10501 aacaaagggc ggatggtgcg tctggcggaa tacagccgtg attgcctgaa agcattgcgc
    10561 gccgaaacca atattcagta tgaagggcgt cagggtggga cgctgcaact gttccgtacc
    10621 gaacaacagt atgaaaatgc gacccgcgat atcgccgtgc tggaagatgc cggcgtaccg
    10681 tatcagctgc tggaatccag ccgcctggcg gaagtggatc ccgcgctggc agaagtagcg
    10741 cacaaactga cgggcggcct gcagttaccc aatgatgaaa ccggagactg ccagctgttt
    10801 acccagaatc tggcgcggat ggcagagcag gcgggggtta aattccgctt taatacgccc
    10861 gttgaccaac tgctttgcga cggcgagcaa atctacggcg tgaagtttgg cgatgaagtg
    10921 attaaggccg atgcgtatgt gatggcgttt ggttcttact cgacggcgat gctcaaaggc
    10981 attgttgata ttccggtgta tccgctgaaa ggctactcgc tgactattcc aattgcgcag
    11041 gaagatggtg cgccagtatc caccattctt gatgaaacct acaaaatcgc cattacccgt
    11101 ttcgataacc gcattcgtgt tggcggaatg gcggaaattg ttggttttaa caccgagctg
    11161 ttgcaaccgc gtcgtgaaac gctggaaatg gtggttcgcg atctctatcc acgcggcggt
    11221 catgtcgagc aggcgacttt ctggacgggt ttgcgcccga tgacgccaga cggtacgccg
    11281 gttgtcggga gtacacgctt taaaaatctg tggctgaata ccggtcacgg cacgctcggc
    11341 tggacgatgg cttgcggttc cggtcagttg ttaagcgatc tgctctctgg tcgcacgcca
    11401 gcgatcccat atgaggatct aagcgtagcg cgctacagcc gtggatttac gccatcacgt
    11461 ccgggccatt tacatggcgc acacagctaa ggaaacgaga tgacccgtcc gatacaggcc
    11521 agcctcgatc tgcaggcatt aaaacagaat ctgtccattg tccgccaggc cgcgccgcac
    11581 gcgcgcgtct ggtcggtggt aaaagcgaac gcttacgggc atggtattga gcgtatctgg
    11641 agcgcgctcg gggccaccga tggctttgca ttacttaacc tggaagaggc aataacgtta
    11701 cgtgagcgcg gctggaaggg gccgatcctg atgctggaag gatttttcca tgctcaggat
    11761 ctggagattt atgaccagca ccgcctgacc acctgcgtac acagcaactg gcagctcaaa
    11821 gcactgcaaa atgcgcggct aaaagcaccg ttggatattt atcttaaagt gaacagtggg
    11881 atgaatcggt tgggcttcca gtccgatcgc gtgcttaccg tctggcagca gttgcgggca
    11941 atggcgaatg ttggcgaaat gaccctgatg tcgcattttg ctgaagcgga acatcctgat
    12001 ggaatttccg gcgcgatggc gcgtattgag caggcggcgg aggggctgga gtgtcggcgt
    12061 tcgttgtcca attcggcggc gactctgtgg cacccggaag cgcattttga ctgggttcgg
    12121 cctggcatta ttttgtatgg cgcttcgccg tccggtcagt ggcgtgatat cgccaatacc
    12181 ggattacgtc cggtgatgac gctaagcagt gagattattg gtgtccagac gctaaaagcg
    12241 ggcgagcgtg tgggctacgg cggtcgctat actgcgcgcg atgaacagcg aatcggcatt
    12301 gtcgccgcag gatacgccga cggttatccg cgccatgcgc ctaccggtgc tcctgtttta
    12361 gtggacggcg tgcgcaccat gacggtgggg accgtctcga tggatatgct ggcggttgat
    12421 ttaacgcctt gcccgcaggc ggggattggt acgccggttg agctgtgggg caaggagatc
    12481 aaaattgatg atgtcgccgc cgctgccgga acggtgggct atgagttgat gtgcgcactg
    12541 gcgttacgcg tcccggttgt gacagtgtaa cttgttgtaa gccggatcgg aggcagcgtc
    12601 ttctggttgc gaaaaaatca tccatccggc tggtcagcaa ctgtagttgt taatgtgaca
    12661 gagccattgc ccatgatagt gtccattaaa agaatggaca ctatttcccc ggaacctgaa
    12721 ctcgccgcac aggcgttcta cataaaacgc ttacgcttca ttgttgactc ggagtcgact
    12781 tcagataaat cacgcttcac ccttgatgca gattcgggtt catgttcaga ttgttgatta
    12841 acaggctgcg agtcctgttg tctttccaga ctcaacaggc ccaactgttg atccaggagc
    12901 tccaggtcct cctgtacctt cacgcgctcc aggagttccg ggtcatccag gaactccaga
    12961 tccataaggt cctccagctc ctcgagtcct tccaggtcaa cctagccttc cttgtgcaaa
    13021 aagtccaaaa ggtccttcag gccctgctgt tttttcaggc cctcgacggc tttgagcagc
    13081 gtcggtaaat tgtctttgtc ccacgggtta ccattacagt caaacacctt caggctctgg
    13141 ggctgcggca cctgtaccgt tttaaacttg ttttctccac agtagtaaat tattagtttg
    13201 ggcggcagcc gtggtgaaga gatcaaatcg ttactagaac agtcaaatac tttcaggcga
    13261 gtcaagttct ccagcatctc atcaggtatt gagttaatat tgttgccggc acaggatagt
    13321 ttatacaggt actggggcat cgacggcggt aaaaattgca agaagttttc agaacagtta
    13381 atcactctca ggttagtcga cagctcaggc agccagataa tactgttttc atagcacttc
    13441 agtaccatca gttttgaggg ctgcaccttc ggtaacaaga tcaagacgtt atctgtacag
    13501 tcaagcacca gcagccactt tggcatcacc ttcggtaacg cggtcaacac gtttttactg
    13561 cagtaaaatt caatcagatg tggaggcaaa ttctcaggga gtgaagtcaa tcccaggttg
    13621 ctaagattga gcatactccg tcttgtaatg agacattcct tcattctgcg caatgcttct
    13681 gcccggtttt caatccggct tgctggcagg ttttcttcca ggatattttc cttgatttct
    13741 tccagacttt ctttcaggtc ttcttgggga atttctgact gtttgttttg ctggttttct
    13801 tccaggattt cgtacaggtt tttttgcagg ttttcttgca aaccatcctg ctgtatagcc
    13861 cattcgcacc atttggcgac catcatgaac tcgttatgtt gctgattcag acgagtcccc
    13921 aacgtgatga tggtattgtt gtccacctgt atgttcactt tgcccgcgat actgaggtca
    13981 atgatctgcc agggggcatc accccagctg aatttttgcg ggctgagata cttcatgttc
    14041 tgcaaaattt gattcttgag aagagccgcc ctggcgggcg tcgtacgcct gggttaacgg
    14101 aagatggggg aatggcgggg gcgaatgggc agatgtcttt aaagctagcc ataatgctac
    14161 gtttccttga aataaatatc ctctggcagc gccggagacg tgaatgtggt caccgcaaag
    14221 gagccacagg acgtcatttg cgctgatatc actactcatc gttgactttt ttcctggtca
    14281 gaaatgccgt gtcgggtctg gtacatgctg tattacggcc taggtagctc tgacgtatta
    14341 aagaaaatac gagagtgaac gacccggaaa taatcgttat taagcaaagg aataactata
    14401 agaaatcgct catgcatccg gtgctacagg cggatgaaac gcctattgtg tgggctatgt
    14461 cagtgcggca ggttgcgtgg gggaatatgt tcaggcgatg tcaggcaggg gcccctgatg
    14521 gttgaccggt aatgcggggc tggtttttat tacctgttac agtcggaaaa agcccttacg
    14581 ctgactttct tgagccggtg ttttgagttc attagccaga gcctgcaatg gtttgtcatc
    14641 cataatttaa ccttcttctg atgttgggat gagcatatca aaatcaggaa atgacaagtt
    14701 tattacaggc tcaggctgcg ttcagtaaag catcgcatcc ggcgatagat gctgcattaa
    14761 atttaagatt cagcttcctc ttcagccacc cgaacgccaa tcttcaacac ttcattatct
    14821 tctttctcgg ccaccgtcca gatcatcccg gcaaactcta cctggtcacc gacaaccggt
    14881 gccgcgccta gcaactgctg gacaatttca cccagcgttt gctgtttatc acgatattct
    14941 cgcccgtctt ccaggccata tatcagcgca acatcagcat atttggcgct ggcttcgaga
    15001 atgaagtcac caaagaagcg ttgatccagc gcgaccggtg gcgactggct gaacagttta
    15061 ccgagcgccg ggagatcgcg ttcccgacca attacacaca atacatcgcc ttcgcgcagt
    15121 cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa acagggctgc aatacgcgtc
    15181 tcttttggca tatgcaaatc gcgcagtgcc gcacctacac accatttatc agcactcaat
    15241 tgataaacaa actgctccca cggattttcc ggatgaatat ccaggccaac gcgtgacacc
    15301 ggacgtccga cgggcggaac caccactttg gcttttttcg ccgcccacga gagtgatgtt
    15361 ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga cattaaagaa cagtcgcgca
    15421 ttctccagcc ccgccatcat cgggaacacg gctaggatga ttggcaccgc gccgcgtaat
    15481 cccacccagc tgataaacac gcgctcacgc agattgaaac cgcggaaagg gagcaatcca
    15541 gcaaataccg aaaggggacg ggcgaagaat atcatccatg ccgacaaaat gagcgccgga
    15601 atggcaatag gcagcagatc gcttgggtta accaatagcc ccagcaccag gaacatggcg
    15661 atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga tgccgtagcg gttgcgaatc
    15721 ggccgattac ccagcagaaa accgcacaga taaacagcca gaataccgct gccttccagc
    15781 gccgtggtta aagcaaaaat taaaataccg ccgcttaatg ccagcaatgg atataatccg
    15841 gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat aaccaccgcc aagaccgatg
    15901 acaatcccga ggccaaattg ctgcagaata tcgaccacga acatccaact gacactgctt
    15961 tcatgctgct ggatcattgc aatcagggta atcgtcagaa agaccgccat tggatcatta
    16021 ctgccggatt caatttccag cgtcgagcca acacgttcgt taagcccctt accacccagc
    16081 agagaaaaga ccgctgcagc atcggtggag ccgacgatag cgccgattaa taagccttca
    16141 atcaaatcaa gattaaacag ccacgccgcc atcatgccgg ttaaaccaga ggtgataagt
    16201 acgcccagcg tcgccagcga cagcgccggt cctaacgcca cacgaaagga actggcctga
    16261 gtgcgcatcc cgccgtcgag caaaataatc gccagcgcca ggttacttac catgtaggcg
    16321 aaggggtaat tatcaaacgg gataccgccg acgccatcga ctcccgccag catgccgatc
    16381 gccagaaaaa taaccagaat aggaatgcca agacgggaag aaaatgaact aagtaatata
    16441 ctgctggtga caaggatgga tcctaagatg aaaaggctaa ttattgttgt ggcatccaat
    16501 ggtcggttac tcctgattac gctgtctctt atataaaccc taccatatta gcggcagaga
    16561 cagcgtttta cttagccctg aagcgtgatt tttttacatt ttaagaacag gatgaccgct
    16621 aatcgtcaac tgagcgcctt cccgggtgtt attcagaatg gcatgtgcgc ccaacggcag
    16681 cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta atgagtggaa tcgacaggcg
    16741 ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct gcgtcataat cattgggcgt
    16801 gctgccgcta aagctaccga gaataatcgc cttctgacgt ggcaaaattc ccgcatgata
    16861 gagctgcaac agcatacgtt cgacgcggaa agggtgcacg ttaatatctt ccagcaccag
    16921 aataccgttc tcaatttttg gcatccacgg tgtaccaatc agtgaaatca gcatcgcaag
    16981 attgcctccc cacaacgtgc cttcggtctg acatgtcggg ccttcccctt gccattcaat
    17041 ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc tcggtaaagg cgttcagttc
    17101 atccgcacca aagtttgcta ccagcatggg gccgctaaag gtgatgacat tgccttgcgc
    17161 cagaagaccg cactgaatgg cggtaaaatc gctatgtccg caaatgagta acgggtcatg
    17221 ttgttggcga gccaccagtg cctgccagtc aatatctgcc agtaaacgac tggcaccgta
    17281 accgccgcgt acagccagca cgatggtgtt gggggtagtc agtctggcaa gggaattgag
    17341 atcttccaga cgctccgttt ccgtacccgc aaaacgctca caacgacggg caataacctc
    17401 gacgttattg acttgatgcc ccgcgtcggt aaggcgctgg ataccacgca gcgcggcgtg
    17461 ctgtttaatg ctgtaacccg atggggcaat taagtgaaac agagacatgg caattccttg
    17521 ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg tgccgttgtc acctcaacgg
    17581 cgattccagg ctataaggat agaagaagtg aaattgagat ggtttgcctt tttgattgtg
    17641 ttattagcgg gttgttcatc aaagcatgac tatacgaacc cgccgtggaa cgcgaaagtt
    17701 ccggtgcaac gtgcgatgca gtggatgcca ataagccaga aagccggtgc agcctggggc
    17761 gtcgatccac aattgatcac ggcgattatc gctatcgaat cgggtggtaa tccgaacgcg
    17821 gtgagtaaat cgaatgccat tggtttgatg cagttaaaag cttcaacctc cggacgtgat
    17881 gtttatcgcc gtatgggctg gagtggtgag ccgacgacca gcgagctgaa aaatccagag
    17941 cgtaatattt caatgggggc ggcttacctg aatattctgg aaaccggccc gctggcaggc
    18001 attgaagatc cgaaggtact gcaatatgcg ctggtggtgt catacgctaa cggggcaggt
    18061 gcgctgctac ggactttctc gtcagatcgg aaaaaggcga tcagcaaaat caacgatttg
    18121 gatgctgacg agttcctcga tcacgtagcg cgaaatcatc ctgcgccgca ggctccgcgc
    18181 tatatctaca aacttgagca ggcactggac gcgatgtaaa tcagtcgcgc actttgtccg
    18241 ctttttcccg ggcttctcgc tcgagagaga aaataatccg ctgtaattgc cgctccaccg
    18301 tcgggctgac gttaagaaaa cggaagctca gacggggagt ggtgatggtt tcattcttgc
    18361 catcaatcac tttgcgctcg ctggtggaga ttaactgggc gtcaaagtga aaaacacccc
    18421 attgccccat gttgacttca atttgagcga agcgcatacc ttcttgtaat tcggcaggct
    18481 ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa atcatacaag cggaaacgta
    18541 acgtactgtt atccgccagt ttggtctggc aaaaataagg cggatggagt ggggcggaga
    18601 tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg aggcggtacg gtaataaatg
    18661 ccggaagctg caagtattca ctctgctgta gttgttcaac agtaaactcg actttcgcac
    18721 cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag cacggcgatg ttgtcttcgg
    18781 cttgactgcc gaaatccagc accagtttat ccggggttat tgccagtatt ttgctgatca
    18841 gctgcccgcc attccaactg agacgcaaag gaattgcggc tttgtgcaaa tcgcgtaaca
    18901 cgcccaggac ggctaacgga ttttgtttca ggaactgctc atggtaatga ctcacgcgga
    18961 aaaactcctg actgacaaac tgtctttggt ttatcggtca tagttaacaa aacttaatac
    19021 aaatgcgtga atatttttta catgttgttc ttaaatcagc cgacaaacgc ccagcatcga
    19081 tcccgccctt gcctatactt agagcgttga cgtaagcatt tcttgcgacc gattcatcga
    19141 aacgagggcc tgaacatggg aattattgcc tggattattt ttggcctgat agccggcatt
    19201 atcggcaagc taatcatgcc ggggcgtgat ggtggtggat ttttcctgac ctgtattctc
    19261 gggatagtcg gtgcggtggt cggcggctgg ctggcgacca tgtttggcat tgacggctcc
    19321 atcagcggtt ttaatttgca cagcttcctg gtggcggtgg tgggcgctat cctggtgctg
    19381 ggcgtattcc gcctcctgcg aagagaataa gattttcata aggcggatag cgatacagat
    19441 gccgctatcc gctttcacat cagaacgtat actcgacacc tgcccagaaa ttgcgacctt
    19501 cttcaataaa cccttcactg taggcatatt gggtatcaaa caggttattg accgacgcat
    19561 tgacgctgaa gccatgacct aaggtgtaat cggctcgaat gtgggtcacc gcaaaaccgg
    19621 cggctttttg tgaaccgtca ctgttgctgt agctggagga acgcgcctct tccgacagcg
    19681 ttacgcttaa cggctcccat ggtttgagtg tcatccatgc ggtcattgtc tgcgttggaa
    19741 gatcggttat cttgccgatg tctttgcgtt tggcgtcagc gtggatcaga gcgtagctca
    19801 atcctacatc cagtatattg ctgattttcc ccttaatacc ggcatccaga ccgctgtaat
    19861 ccaccgtgcc gctgttctga ttttgaatgg tatcggcatt gatattgtgc gagaggatgg
    19921 catcactcac ccggttatag taaacgctga cctcaaatcc ccagtcgcgc gtgaaggcac
    19981 cattccaggt taaatccacc ccgcgagcgc gttccggctt gagctgcggg ttaacaatcg
    20041 ctatctggtt atacgcaggt ttggatgtgg tatagcgttc tttcagcgtc ggaaagcgtg
    20101 tgcggtcata gtacgaaagc gccagagtgt cttcattggc aaagtggtat ttccccatca
    20161 cctgccagtt aaaagctgac tgattgttgt cgtcataatg ggtgatgctg ccatctttct
    20221 cgtgtttttt cgcttctacg ctatcgcgcc agtcatagct gattcccgcc acgacatcga
    20281 cattatcagc agcagcccat tgatattcac tggcgagcga ccaggtacga tcttcatagc
    20341 gatcgtaagc ggcgtgcggc gcaccttttt cccggtgtac gtcatctttc cagttaacgg
    20401 caaacgacag cagatcgttt tcacgcacat cggctgccag ttgtaatccg gcaccgtcgc
    20461 tgtaatcgga ataatggctg tagctgcctt ttttattttt caaatcagcc agcgagttgt
    20521 acatcatcag cgtattttca aaggtgtcgc gatacagccg acttttcagg gtaaaacggt
    20581 cgtttagttg ggtcgctccc tgataataaa aactttcttt gtcatactct ggccactgcc
    20641 agtagcgtga tttttgacca caatttccgc tgtatggcgg gttatctttt tcaccatcct
    20701 gcttaatgta agtcagcgtg tattcatcgt tttcgcgcgg tgtaaaaccg agcttcacaa
    20761 tgccgcgttt atcatcagcc gatgaattaa tcatcttgcc gtgtttgcct gcaatatcat
    20821 tatttacacc atgcggcagg ccgagaaaat cctgctttag ctggctaccg ctgacttgca
    20881 aatacccaag atcgctgctg gcggcaaatg aagcatgcat atcgtaggca ttgtcctgac
    20941 tacggctcca tccctggcga tatcccagac ttgcttcttc agggtggtga tattaatggc
    21001 tccgcccatc tgattaggcc catgaagcag cgacgaatac cctttggaaa cttcgactgc
    21061 ccccagattg ttggtcagaa tccgcgccag atcgaggttg ccgtcatagg ggacataaat
    21121 gggcacaccg tcgaaataga ccggcacctg ccgactatca aagccacgaa cttttacctg
    21181 ttcttcgttg cggctgcctg acttttgcag caccacgccg gggacgacac ttaatgcctg
    21241 agcgacattc tgtttatcca aggccttcat ggtgggttga tcgagaacgg tggtcgtcgt
    21301 cgatgatacc ggactggacc atacggtcag cgtgtcgctc tcctgagccg ctacggcctg
    21361 ctgggtaaag gcaagtgata atgctgtgca gagataacgt tttttgagtc tcatagaata
    21421 aattccgttt aacaaagaat taaatagagg ctgtgggttg cagactgact ttaatgtcag
    21481 ctggtgccgc gtaataaggc gcggaggtaa taaaaaggcg aatgccgcag tcgaggtaat
    21541 ttttgagtgt cgtcagatta attccgccgg tcagagccag cgtgcagtga ggagccagcg
    21601 acggcgctat ttgcgcaatt tcagttgcct gttgcggact aaatttgtcg agctgaagta
    21661 cgtctggttg tgcgcgtaac gcggcgatag cctctttcgg cgtgtcggct tcaacaacaa
    21721 ttttcttctc gggtgcgtgg cgacgtaact gagcgattgc gcctgaccag ccctgattgt
    21781 catgaagaaa atggcggtgg ttggcaaaca ataatatggt ttccgcacat ccggcacgat
    21841 gaattagtcc tccggcagcc agaattgcct gcgaagccag taagcgggtg cccggaattg
    21901 cttttcgggt gcaggcgata ttgccgtcag gataacgttc acgaagcaat gccagcattt
    21961 gagcgagata atcagaaaca ccgcaactcc actccagcac attctggact gccttccatc
    22021 cctgatgaag tgctgcggca ttaccctgcg cacggattag ccgttgaccc gcgttcgctt
    22081 gtgaaccgtc gctgaccgcg tcatcaatag ttaatcccag cgtagttaac atcttacacg
    22141 cgacagaaac gccgctgacg cagccgccct gacggtgaaa aaacgctata tagccaggct
    22201 ggtgtccaat atttaatgct cgcgtggtca ggtcacctcc ctggatatct tccagcagta
    22261 acgcatcgat ttgtgcctga gaaaggaaaa tcatcacagt gcctcctgcg gtacgctatc
    22321 ccaccagatg agcgcccagt cgcggctggc aggggcgagg gcacgagcat cttgttgttg
    22381 ataccagtga tataaccgct cacgctcatc gtcgttaagc gcgccaagac tccagctgac
    22441 gttctgctca aaacgttccc aggtgctgtt atcctgttgg caattttgtc cacggataaa
    22501 atcaacgtgg gcataaattc ccatctgata caggacgttg agcgcaaaga tatagttggg
    22561 taattcgatc acttcccgac cagccgcccg ctgaatggct ggagagacga acgaggtgct
    22621 gaccagatga gttgtataga cgcgaagccg tgcctgatta ttcagtttgc tcattgcatg
    22681 gcgcatatca gccaccagag tggagcggga agccacggca atatcgcagc gtggcaggtc
    22741 actccagtcc tcttcccagg cgcgctggat ccagtggaca ttgtcagcct tcagtgcggc
    22801 ggcgcggcgg gcggcaacgt tcagcatccc ctgactgtaa tcaacgccat aaaccgtagt
    22861 gagtttgtca gccagcgcca gcgatacggt gcctggtccg cagcccatat cgaataaggt
    22921 ctgagcgcct tgcaggtcaa ttttagcgat cagttgttgc aggtagctgt ccgttggcga
    22981 ggcgcaattt tccgccattt tctccgcccg ttgatcccag tgatcgggcg ttttttctgt
    23041 gcggtgagcc agtttgagtt gctgcagata aaggtctgca aaatcaatat catcaatgag
    23101 catgattgtt ccttagcggc taattgcaga gaggtgatga tgaatttggt cggcagaaac
    23161 ccgatacagg gccgccagtt tgttcgttgt taactgttcg gtcggtaagc cttgtgttac
    23221 tcgtccatct ggctctactt ggataatgct gtcggcaatg gcgttggcat ggagcgtatg
    23281 gtgagtcgac attaacattg tcatgccgtt atttttgagc tgcgccagcg tgtccagaag
    23341 ttggatctga tgaccgaaat cgaggctgga ggcgggctca tccagtaaca acaagcgcgg
    23401 ttgttggacg agtgcacggg cgattaaagc cagctgacgt tcaccgccgc taagcgtatt
    23461 ccagcgtcgt gtcgccagat gcaaaatgcc cagcttttcc agttgctccg tggctttcag
    23521 gcgttcttgc ttaccgggaa cggaaaaagc gcctatggtg ggggccaggc ccatcaacac
    23581 catatccagc acactgaaag caaaaatacc gtcgtgcgcc tgcggcaccc aggcaatagc
    23641 ctgtgcgcgc tggcgatggc tgagtgtctg cacagagagg ctatccagta ggatctcgcc
    23701 ttttaataac ggtataacgc caagaatgga gcgcatgagt gtggttttgc cgcaaccatt
    23761 cgcgccaagc agacaacaaa ttgttccttg cgacagggtg aaggacacgt cgcgtagcac
    23821 tggatggcgg gagtaaccca gagacgcatg acgaacggca agcaaagtca ctggcgacct
    23881 ccgcgtaaca acaaaagcag gaaaaacggt gcgccaacaa aagcggtcag aatgccaagt
    23941 gggatttctg ttgttccaat gctgcgcgcc agcgtatcgg taagcaggag cagaatagcg
    24001 ccagtacaca ttgccatagg caacagctgc tgatggttat gaccggtcaa caagcgggca
    24061 atgtgcggaa cgaccagacc gatccaacca attatgcccg ctattgctac cgtgctggcg
    24121 gtgattagtg tggcgcagac aatcaatcca aagcgcagac gcgtcacgtt cagtcccagc
    24181 gaacgggctt cgtcgtcgga aagcgtgagt aaattcatgc gccagcggag aaaaaacagg
    24241 ggcaaggagc ctatgagaat gatcggtgca gcatagcaga gatcgcgtaa ggtcacggtg
    24301 gacagtcccc ccagcaacca gaaggtaatt gacggtaact gggtgtaagg atcggccagc
    24361 gttttaatta gcgatatccc cgctccgcac aacgttccta acgcaatgcc taccagcacc
    24421 agggttaaga tcggatcgtg gcgggtcacc cgacgcgtaa tcaaccagac gcccgcgacc
    24481 accatcagcc cgccgcaaaa tgcgtacagc tggatgtaaa caatgggtaa tccccacagg
    24541 attgccgcgc aggcaccaag tccggcaccg gcggagacgc ctaaaatatc cggtgaaact
    24601 aagggattac gcaacattcc ctgataggtg gttccggctc ctgccagcgc tgcgcccagt
    24661 aacagcgcag caagaatacg cggcacgcgg atttgccaga agacaatttg ttcctgcaca
    24721 gactcatttt cttgacgaag aattagcgcc agcagttgct gcatatcgag atgataagcc
    24781 ccacttacgg cggcaaaagt gatactgata gctgccagca gcaaaataca tccgtttacg
    24841 attctcatta gctcgccacc aatttttgat agtcggcatc actgagtgaa caatgccaga
    24901 acagttgggc gtaatgctgc atgtcacttt taaactggcg attgatggcg ggatccagcc
    24961 aggcgtgaag tctgcgtaat cccagaagac ggttgattcc cggcggggca tcgagccagc
    25021 caaagggcaa gccacttaaa aataagatgc gttgttccgc aacggctttc acgccctgcc
    25081 agagaggatc acgacgaata aaatctgcag taacggcctc ctgaaccaga ataatatccg
    25141 gctgccagcg caggagattt tccatggaaa cctgtgtcag gccgtggcga tcggctattt
    25201 gcgcgacgtt gtgcaaacct aataattccg ccgcctcggt atgcaacgaa ccctgtaagc
    25261 ccgtttccag ccctcgggga ccgcgcgcag cataaaagcg gaggttagcg gcgggtgagg
    25321 tggcgaatgc ctgagcttca ccaacgaagt ggctggcgag attggcttgt tctgcggcgc
    25381 ggtgctcttc gcctaacgtt ttgccaagcg ttgttaactg ttctgctgat tgcgccagtt
    25441 ttccgttaag caataaccag gggatttgtg tctgttcgct aacctgccgt gcctgggaga
    25501 tcaaggtttc atccgtattg ccgcaatcaa cgaccaaatc gggatgtaac gccataagcc
    25561 cttccagcga tagtgtgctg gcgcgcccgg cgagtcgtcc cagcctgggt aactggcgaa
    25621 tgtgctctgg caatggaatt aatgcctgac gggcaaaatc aaaagaggaa aaaccgacca
    25681 ttttttctgg cgcgacagcc agcaagagca aatcggcggg cgcgcctgcg ctgacgatgc
    25741 gtgtaatgtt ttgggcttcg ggaagagatt gcgaagcaaa acgattaact gtttgtgaaa
    25801 aggacggtag agactgcagg agcagggtag atgcaagtgc ctgcgcaaac gtccgtctgg
    25861 ttatgggcat ggtgaacatc ctgaaaagta aaatcgatat atactttcat atatatcgat
    25921 aaaaatcatg ttcaatatca aagtttagct attgaaaaca tgaagatgtg acgacttccg
    25981 caagcctgtt gaccctctct atcattaaaa atgtatttat caggtggcta atattattta
    26041 aatcatttaa ggctaaatta tttaaatatg aatgacatta ttgttttttt atcgtccacg
    26101 gttacgcgac gttgaagtta cttgttttat aatgagcgat ttttgatatc tcgccgagaa
    26161 aattatcagc attctctcgt ttcagtgtcg tgaatatact gttaacggag gtgaaaagaa
    26221 tgctgggggt tatacaaaac ctatgttgtt gtcgtccgga ttcaccgctt gctgatagta
    26281 agcagcgtaa ccagaagtta ccgcaacaag actttgagga ttttaagaac gacgttatta
    26341 atgtcattaa caacaatgaa gatatttatt ttcttagaga gagtaattta ctgaatgcgt
    26401 ttattcggac gtgcaaaaaa agagatgata acagttacgg aaatatgata tttacgcttt
    26461 attttgataa cgatatctat gggaacagaa ttatcaaagc atcgctgaac ggcggagagt
    26521 tgcagttatt aagcacaggg tgtagcaatg ataacattct c
//
LOCUS       DADLTY010000042        26282 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.042, whole genome shotgun sequence.
ACCESSION   DADLTY010000042 DADLTY010000000
VERSION     DADLTY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26282
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.042"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..721
                     /locus_tag="JN334_001486"
     CDS             <1..721
                     /locus_tag="JN334_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAY7437670.1"
                     /translation="TAESQRALVGSGIEAQVNGERVLICAAGKHPADAFAGLINELES
                     AGQTVVLVVRNDDVLGIIALQDTLRADATTAISELNALGVKGVILTGDNPRAAAAIAG
                     ELGLEFKAGLLPEDKVKAVTKLNQHAPLAMVGDGINDAPAMKAAAIGIAMGSGTDVAL
                     ETADAALTHNHLRGLVQMIELARATHANIRQNITIALGLKGIFLVTTLLGMTGLWLAV
                     LADTGATVLVTANALRLLRRR"
     gene            complement(823..1068)
                     /gene="tusA"
                     /locus_tag="JN334_001487"
     CDS             complement(823..1068)
                     /gene="tusA"
                     /locus_tag="JN334_001487"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HAY7437671.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            1289..1954
                     /locus_tag="JN334_001488"
     CDS             1289..1954
                     /locus_tag="JN334_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAY7437672.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            2027..2584
                     /gene="dcrB"
                     /locus_tag="JN334_001489"
     CDS             2027..2584
                     /gene="dcrB"
                     /locus_tag="JN334_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HAY7437673.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(2588..3805)
                     /locus_tag="JN334_001490"
     CDS             complement(2588..3805)
                     /locus_tag="JN334_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7437674.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            3939..4988
                     /locus_tag="JN334_001491"
     CDS             3939..4988
                     /locus_tag="JN334_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7437675.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            5043..5630
                     /gene="acpT"
                     /locus_tag="JN334_001492"
     CDS             5043..5630
                     /gene="acpT"
                     /locus_tag="JN334_001492"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HAY7437676.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            5741..7315
                     /gene="nikA"
                     /locus_tag="JN334_001493"
     CDS             5741..7315
                     /gene="nikA"
                     /locus_tag="JN334_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HAY7437677.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            7315..8259
                     /gene="nikB"
                     /locus_tag="JN334_001494"
     CDS             7315..8259
                     /gene="nikB"
                     /locus_tag="JN334_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HAY7437678.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            8256..9089
                     /gene="nikC"
                     /locus_tag="JN334_001495"
     CDS             8256..9089
                     /gene="nikC"
                     /locus_tag="JN334_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HAY7437679.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            9089..9853
                     /gene="nikD"
                     /locus_tag="JN334_001496"
     CDS             9089..9853
                     /gene="nikD"
                     /locus_tag="JN334_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HAY7437680.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            9850..10656
                     /gene="nikE"
                     /locus_tag="JN334_001497"
     CDS             9850..10656
                     /gene="nikE"
                     /locus_tag="JN334_001497"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HAY7437681.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            10662..11063
                     /gene="nikR"
                     /locus_tag="JN334_001498"
     CDS             10662..11063
                     /gene="nikR"
                     /locus_tag="JN334_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HAY7437682.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(11183..11542)
                     /locus_tag="JN334_001499"
     CDS             complement(11183..11542)
                     /locus_tag="JN334_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HAY7437683.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(11539..11814)
                     /locus_tag="JN334_001500"
     CDS             complement(11539..11814)
                     /locus_tag="JN334_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAY7437684.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(11873..12997)
                     /locus_tag="JN334_001501"
     CDS             complement(11873..12997)
                     /locus_tag="JN334_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAY7437685.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(12997..15732)
                     /gene="rbbA"
                     /locus_tag="JN334_001502"
     CDS             complement(12997..15732)
                     /gene="rbbA"
                     /locus_tag="JN334_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HAY7437686.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(15729..16796)
                     /locus_tag="JN334_001503"
     CDS             complement(15729..16796)
                     /locus_tag="JN334_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7437687.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(17162..18784)
                     /locus_tag="JN334_001504"
     CDS             complement(17162..18784)
                     /locus_tag="JN334_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY7437688.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(19046..20635)
                     /locus_tag="JN334_001505"
     CDS             complement(19046..20635)
                     /locus_tag="JN334_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY7437689.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            21133..22052
                     /locus_tag="JN334_001506"
                     /pseudo
     CDS             21133..22052
                     /locus_tag="JN334_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908812.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
     gene            complement(22139..22654)
                     /locus_tag="JN334_001507"
     CDS             complement(22139..22654)
                     /locus_tag="JN334_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437690.1"
                     /translation="MTKLMQFVQRCYYMTNKKMYFILILVFTLLQVCFFALWKARDGS
                     TTSLECTSTLTRNAKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFF
                     TYKIDSNGRISGNAKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSI
                     PGLIINTCAPI"
     gene            complement(22608..23372)
                     /locus_tag="JN334_001508"
     CDS             complement(22608..23372)
                     /locus_tag="JN334_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7437691.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            23618..24379
                     /locus_tag="JN334_001509"
     CDS             23618..24379
                     /locus_tag="JN334_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7437692.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(24486..24998)
                     /locus_tag="JN334_001510"
     CDS             complement(24486..24998)
                     /locus_tag="JN334_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437693.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
     gene            complement(25137..>26282)
                     /locus_tag="JN334_001511"
     CDS             complement(25137..>26282)
                     /locus_tag="JN334_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7437694.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT         6646 a         6672 c         6151 g         6813 t
ORIGIN      
        1 caccgccgaa tcacagcggg cactggtcgg gtctggcatt gaagcgcagg ttaatggtga
       61 gcgcgtgttg atatgcgctg ccgggaaaca tcccgctgat gcatttgctg gtttgattaa
      121 tgaactggaa agcgccgggc aaacggttgt gctggtagta cgtaatgatg acgtgctggg
      181 tatcattgca ttgcaggata ccctgcgcgc cgacgctaca actgccatca gtgaactgaa
      241 cgcgctgggg gttaaaggag tgatcctcac cggcgataat ccacgcgcag cggcggcaat
      301 tgccggggag ctggggctgg agtttaaagc gggcctattg ccggaagata aagtcaaagc
      361 ggtgaccaag ctgaatcaac atgcgcccct ggcgatggtc ggtgatggta ttaacgacgc
      421 gccagcgatg aaagctgccg ccatcgggat tgctatgggt agcggcacag acgtggcgct
      481 ggaaaccgcc gacgcagcat taacccataa ccacctgcgc ggcctggtgc aaatgattga
      541 actggcacgc gccactcacg ccaatatccg ccagaacatc accattgcgc tgggactgaa
      601 agggatcttc ctcgtcacca cgcttttagg gatgaccggg ttgtggctgg cagtgctggc
      661 agatacgggt gcgacggtgc tggtgacggc gaatgcgtta aggttgttgc gcaggagata
      721 aggcaaaccg atcgcaacat tgagcgcgat cggtcccctc gcccctctgg ggagagggtt
      781 agggggtgag gaaaaggcgg catcgaagcc aatcagcccc tatcaaccgc ctttacgaat
      841 caaataacga taaggcagtc catccgtctc tttcgcaacc agttcgtgtt ccataaaggt
      901 acaaaaccca ggaatatcgc gggtagtggc cggatcgtcg gcgataatca gcaacgtttc
      961 gccaggctgc atattgcgca cggttttgcg caccatcatc accggttccg ggcagcgcag
     1021 gccaagcgcg tcgagtgtgt ggtcagggct ggaaaagaga tcggtcattt tcttctcatc
     1081 acttaaaaaa acggcgctag tttacgccct gtgagtccgt aagcaaacca ggttaacgat
     1141 tgcgtgaaaa ttaaccattg cattgtcaac gtaaagcagt atcatgcggc ggctcgaaaa
     1201 aagggtaagc acgttattat gttaaggtaa cagacgtgtc gtacgtattg ggttccctca
     1261 ccccaatggt taatcaaaaa ggtacaatat gaacgttttc tcgcaaactc aacgctataa
     1321 ggcgttgttc tggttatcgt tatttcatct tctggtgatc acctccagta actatctggt
     1381 tcagcttccc gtctccattt ttggtttcca taccacctgg ggcgcgttta gctttccgtt
     1441 tatttttctt gctaccgacc tgaccgtgcg tatttttggc gcaccgctgg cccgacgcat
     1501 tatcttcgcg gtaatgatcc ctgcgttatt aatctcctac gtcatctcgt cgctattcta
     1561 tatgggttcc tggcagggat tcggcgcact cgcccacttc aacctgtttg tcgcccgtat
     1621 cgccaccgcc agtttcatgg cctacgcgct ggggcaaatc ctcgacgtgc acgtttttaa
     1681 ccgcctgcgt cagagtcgcc gctggtggct ggcaccgaca gcgtccacac tgttcggtaa
     1741 cgtcagcgac acgctggcct ttttcttcat tgccttctgg cgtagcccgg atgcctttat
     1801 ggctgaacac tggatggaaa tcgcgctggt cgattactgt ttcaaagtgt taatcagtat
     1861 cgttttcttc ctgccaatgt atggcgtatt actcaatatg ctgttgaaaa gactggcaga
     1921 taaatccgaa atcaacgctt tgcaggcgag ttaaaggttc gttatcagag ttgtgataag
     1981 atggatgaat gagccgttat ggccgtttat cgaaaggaag aagtcaatgc gcaatctggt
     2041 taaatatgtc ggaattggcc tgctggttat ggggcttgcg gcctgtgatg ataaagacac
     2101 taacgctacg gcgcagggtt cggttgcgga aagtaacgct accgggaatc ccgtcaacct
     2161 gcttgatggc aagttaagtt tctcgctgcc agcggatatg accgaccaga gcggtaagct
     2221 gggaacgcag gccaataaca tgcatgtctg gtccgacgcc accgggcaga aagcagtcat
     2281 cgtcatcatg ggcgatgatc cgaaagaaga tctggcggtg ttggcgaagc gtctggaaga
     2341 tcagcaacgt agccgcgatc cgcagctgca agtggtaacc aataaagcca ttgagctgaa
     2401 aggtcacaaa atgcagcagt tagacagtat tatctccgcg aaaggccaga cggcgtactc
     2461 ttccgttatt ctgggtaacg tgggtaatca actgctgacc atgcaaatta cgctgccagc
     2521 tgacgatcag caaaaagcgc aaaccaccgc agaaaacatc attaatacgc tggttattca
     2581 gtaagtttta agatgatgag gcggcctcag ggacgtgttc cggaggccgt ttttttaatc
     2641 gccacgtcag taataacgcg attgcgacca gtcccgccgc cgccagataa atcaccggta
     2701 cgcccgccca gctcatcacc agcccagcca gtggtccagt cacgccaagc gataaatcca
     2761 taaatacggt gtaagttgcc agcgccgccc cctgattttg ctgcggaacc gcttttaccg
     2821 ccactacacc caatgccggg aacaccagcg aaaacccggc ccccgccagt aagacgccga
     2881 ttttcgccat ccacggcata gtcgccacgc caaccagtag caggccgatt atctcaacgc
     2941 taaagcaaat catcgctacg tttaagccac cgatacggtt aatgccgtta gggaataaca
     3001 aacgcgtacc gacaaacgca cagctaaaca gcgtcagcgc gaaagccgca ccgtcccaac
     3061 ctttagcgtc ataaaacagc gtgataaagg tggcgatgac gccaaatccg gcggaagcca
     3121 gtgccagcgc cataccgtac agccagacgc gcccaagcac cgcgcgaaac ggcagcggtt
     3181 tgcctttact ggcttttacc gtcggacgcg ggatcgccaa caaaatggcc accagcgcca
     3241 cgcccataat gattaacgct aacgcctgca agccgcccca gtgataaaac acgacgccta
     3301 acggcgcacc catcgccatc gccccgtaag tgacaatgcc gttccacgaa atcacccgcc
     3361 cgatatgcag cgagccaacc actccaacgc cccatagggt cgatcccgtt ccggcaaaac
     3421 tttgcccaat cccaaggatg acgcgcccca ggcaaagtaa taacaggctg atgacaggca
     3481 gactggcggt taatcctgcc gtcagatacc ccagaccgct caaaaagcag ccgcataaac
     3541 cgaagacgac aatctttttg ggtcccagca aatcggcgta acgtccggca tgagggcggc
     3601 tcagcaaggt ggcgaaatat tgcaggctga taaccaatcc tgcccagaag gcgctaaagc
     3661 ccatcacatc atggacatag cccggtaata cagcgagcgg caacccgatg gtgaggtagc
     3721 tggcgaagtt aaacatgaca atggagacaa tgcgcaaatt caggcgcaat ccgtttagcg
     3781 cgggttcggc tacgggttcg ggcatgagga tcaccacatt tttacaacag tgtttcattt
     3841 ttaccatgtg ctgacgtgaa aatcagcagt aagaatcaga atattgctgg cgtgactccc
     3901 gcgctacact taacacaaaa agtcacaagg aagccccaat ggaaacccct caacccgata
     3961 aaacgggcat gcacattctg ctcaagttgg cctcgctggt agtgatcctc gcgggcattc
     4021 acgcagcggc agatatcatt gtgcagctgt tactggcgct gttttttgcc atcgtcctca
     4081 acccgctcgt tacctggttt attcgtcggg gagtacaacg ccccgttgcc attacgattg
     4141 tagtggtggt gatgctgatc gcactaaccg cgctggtcgg cgtactggcg gcatcgttta
     4201 acgaatttat ctctatgctg ccgaagttta ataaggagct gacgcgcaaa ctttttaaat
     4261 tgcaggagat gttgcctttt cttaatttgc atatgtcgcc ggagcgaatg ctgcagcgga
     4321 tggactcgga aaaagtggtt accttcacca cagcgctaat gaccgggctt tccggggcaa
     4381 tggcgagcgt gcttttgctg gtgatgaccg tagtttttat gctgtttgaa gtgcgccacg
     4441 tcccttacaa aatgcgtttt gcgctgaata acccacagat tcacatcgca ggattacacc
     4501 gcgcacttaa aggcgtttcg cattatctgg cgttaaagac gctactcagt ttatggacag
     4561 gtgtcatcgt ctggctgggg ctggcgctaa tgggcgtaca gtttgcgctg atgtgggcag
     4621 tactggcgtt tttgctcaac tacgtgccca atatcggcgc ggtaatttcc gccgtaccgc
     4681 caatgattca ggtgctgctg tttaatggtg tttacgaatg tattctggtc ggcgcattgt
     4741 ttttagtggt ccatatggtc atcggcaata ttttagaacc acggatgatg ggccatcgcc
     4801 tggggatgtc caccatggtg gtatttcttt cattgttaat ctggggatgg ctgctcggcc
     4861 cggtagggat gctactttcg gtgccattaa ccagcgtgtg taaaatctgg atggaaacga
     4921 cgaaaggtgg cagcaaactg gcgattttac tggggccggg cagaccgaaa agtcggttac
     4981 cgggatgagg cgacaagtga tacgatacgc actttcattt tccattaaac gttggccctg
     5041 atatgtatcg gatagttctg gggaaagttt cgaccttaag cgcagctcca ctgccaccgg
     5101 gtttacgcga gcaagcaccg caaggtccac gacgcgaacg ctggctggcg gggcgtacat
     5161 tgctttcgca cacgctttcc ccgctaccgg agatcatcta tggcgaacaa ggcaaacctg
     5221 cattcgcgcc ggaaacgccg ctatggttca acttaagcca tagcggtgac gatatcgctc
     5281 tgctgttgag cgatgaaggc gaagtcggct gcgatatcga agtgattcgc ccgcgcgtca
     5341 actggcgctg gctggcgaac gccgtgttca gcctcgggga acacgctgaa atggacgccg
     5401 tgcatcctga tcagcaactg gaaatgttct ggcgcatctg gacgcgcaaa gaagccatcg
     5461 ttaaacagcg tggcggcagc gcctggcaaa tcgtcagcgt agatagcacc tatcactcct
     5521 cgctgtcagt cagccattgt cagctagaaa atttaagcct tgcgatctgc acccccaccc
     5581 cctttacgct caccgccgac agtgtgcaat ggatcgattc agttaactga tccgcccacc
     5641 cgactgccca tctattgatc cagaacaggt aatcagtatg acgaatactt aaaatcgtca
     5701 tacttatttc cgccatctat tttaatccat tggggttacc atgctctcca cactccgccg
     5761 cactctattt gcgctgctgg cttgtgcgtc ttttatcgtc catgccgctg caccagatga
     5821 aatcaccacc gcctggccgg tgaatgtcgg gccactaaac ccgcaccttt acacgcctaa
     5881 ccagatgttc gcccagagca tggtttatga accattggtg aaatatcagg cagacggttc
     5941 ggtgatcccg tggctggcaa aaagctggac tcattcagaa gatggtaaaa cctggacctt
     6001 caccctgcgt gatgacgtga aattctccaa cggtgaaccg ttcgatgccg aggcggcggc
     6061 agaaaacttc cgcgcagtgc tcgataaccg tcaacgtcac gcctggctgg agctggcaaa
     6121 ccagattgtt gatgttaaag cactcaataa aacagagctg caaattaccc tgaaaagcgc
     6181 ctactatcct ttcctgcaag aactggccct gccccgtcct ttccgtttta tcgctccctc
     6241 gcagtttaaa aaccatgaaa ccatgaacgg aattaaagcg ccgattggca ccggaccgtg
     6301 gattttgcag gaatcgaaac tgaatcagta cgatgtcttc gtccgtaacg aaaactactg
     6361 gggcgaaaag ccagcgatta aaaagatcac ctttaacgtc atcccggacc cgactacccg
     6421 cgcggtggcg tttgaaactg gcgatatcga cctgctgtac ggaaacgaag ggttattacc
     6481 gctcgatacc ttcgcccgct ttagccagaa tccggcttac cacacccaac tgtcacagcc
     6541 gatcgaaacc gtgatgctgg cgctcaatac cgccaaagcc cccaccaacg agctggcagt
     6601 acgtgaagct cttaattacg cggtaaacaa aaaatcgctg attgataacg cgttatatgg
     6661 cacccagcag gtcgccgaca ccctgtttgc cccttctgtg ccttacgcta atctcggcct
     6721 gaaaccgcgc cagtacgatc cgcagaaagc gaaagagttg ctggaaaaag ccggttggac
     6781 gctgcctgcg ggcaaagaca tccgcgagaa aaatggtcag ccgctgcgca ttgaactgtc
     6841 gttcatcggc accgatgcgt taagcaaatc gatggcggaa atcattcagg ctgatatgcg
     6901 ccagattggc gcagatgtct cgctgattgg cgaagaagag agcagtatct atgctcgtca
     6961 gcgcgacggt cgttttggca tgattttcca ccgcacctgg ggcgcgccat acgatccaca
     7021 cgccttcctg agttcaatgc gcgtaccgtc gcacgctgac ttccaggcac agcaaggatt
     7081 agccgacaag ccgctgattg ataaagagat cggcgaagtg ctggcgaccc atgacgaaac
     7141 gcaacgtcag gcgctgtatc gcgacattct gacccgtctg catgacgagg cggtttatct
     7201 gcctatcagc tacatctcga tgatggtggt atcaaaaccg gagctgggta atatccccta
     7261 cgcgccgatc gccaccgaaa ttccgttcga acagattaaa ccggtgaaac cttaatgttg
     7321 cgttacgtat tacgccgctt tctgctgctg atcccgatgg tgctcgccgc ctcggtgatc
     7381 atttttctga tgctgcgcct cggtaccggc gacccggcgc tcgattatct gcgtttatct
     7441 aacctgccgc cgacgccgga gatgctggcc tctacccgca ccatgctcgg gctggatcag
     7501 ccgctgtacg tccagtacgg tacctggttg tggaaggcgc tgcatcttga ctttggtatc
     7561 tcattcgcca gccaacgccc ggtactggac gatatgctga acttcctgcc cgccacgctg
     7621 gaacttgcag gtgcggcgct ggtattaatt ctgctcactt ccgtaccgct cggtatctgg
     7681 gcggcgcgcc accgcgatcg tttgccggat ttcgccgtgc gctttatcgc gtttcttggc
     7741 gtgtcgatgc ccaacttctg gctggcattt ttgctagtga tggcgttttc ggtgtatctg
     7801 caatggctac ccgcgatggg ctacggcagc tggcagcaca tcattttacc tgcggtttcc
     7861 atcgccttta tgtcgctggc gattaacgcg cgactgctgc gcgccagtat gctggacgtc
     7921 gccggtcagc gtcacgtcac ctgggcgcgg ctacgcggcc tgaatgacaa acagaccgaa
     7981 cgccgccata ttctgcgcaa tgcctcgctg ccgatgatca ccgccgtggg gatgcacatt
     8041 ggcgaattga ttggcgggac gatgattatc gaaaacatct ttgcctggcc tggcgtcggg
     8101 cgctatgcgg tgtcggcgat ttttaaccgt gactatccgg tgattcagtg ctttacgctg
     8161 atgatggtgg tggtttttgt ggtctgtaat ttgattgtcg atttgctcaa cgccgcgctg
     8221 gacccgcgca ttcgtcgtca tgaaggagcg cacgcgtgaa ctttttcctc tcttcccgct
     8281 ggtcggtacg cctggcgctg atcattatcg ccctgctggc gctgattgcg ctcaccagcc
     8341 agtggtggca gccgtacgat ccacaggcaa ttgatttgcc gtcgtgcctg ctttcgccgg
     8401 atgcgcagca ctggctgggc accgatcact taggtcgcga tattttctcg cggctgatgg
     8461 cagcgacccg cgtgtcgctc ggttcggtaa tggcctgcct gctgctggtg ctgacattag
     8521 ggctggttat tggcggcagc gccgggttga ttggcgggca cgttgatcag gccaccatgc
     8581 gcgtcgccga tatgtttatg accttcccga cctcgattct gtcgttcttt atggttggcg
     8641 tgctcggcac cgggctgacc aacgtaatta tcgccatcgc cctgtcgcac tgggcgtggt
     8701 atgcacgcat ggtgcgcagc ttggtgattt cactacgcca acgcgagttt gtgctggcgt
     8761 cacggctttc cggtgcgggc catgtgcggg tgtttgtcga tcatctggca ggcgcggtga
     8821 tcccttcgct gctggtgctg gcaacgctgg atatcggcca tatgatgctg cacgtcgcgg
     8881 ggatgtcttt ccttggcctc ggtgtgaccg cgccgaccgc cgaatggggc gtgatgatta
     8941 acgacgcgcg ccagtatatc tggacccagc cgctgcaaat gttctggccg gggctggcgc
     9001 tgtttatcag cgtgatggcc tttaacctgg tgggtgacgc actgcgcgat catctggatc
     9061 ctcatctggt gacggagcac gcacactaat gccgcaacag attgaactac gtaatatcac
     9121 gctacaggcc gcgcagccgc tggtacacgg tgtatcgtta accctgcaac gcgggcgcgt
     9181 gctggcgtta gtcggcggta gcggcagcgg gaaatcatta acctgcgccg cgacgctggg
     9241 cattttgccc gctggcgttc gccagacggc gggggaaatt ttagccgatg gcaaaccggt
     9301 ttcgccttac gccctgcgcg gcatcaaaat tgccaccatc atgcagaacc cgcgcagcgc
     9361 ctttaatcca ctgcacacca tgcacaccca cgcgcgggaa acctgcctgg cgttagggaa
     9421 acccgccgat gacgctacgc ttaccgctgc catagaagcg gtggggctgg aaaacgccgc
     9481 gcgcgtgctg aagctgtacc cgttcgagat gagcggcggc atgttgcagc gcatgatgat
     9541 tgcgatggcg gtgctgtgtg aatcaccgtt tatcatcgcc gatgaaccga ccaccgacct
     9601 cgacgtggta gcacaggcgc gcatcctcga tctgctggaa agcattatgc aaaaacaagc
     9661 gccgggaatg ctgctggtca cccatgatat gggcgttgtg gcgcgtctgg cggatgacgt
     9721 ggcggtgatg tcacaaggta aaattgtcga acagggcgat gtagaaacgc tgtttaacgc
     9781 ccccaaacat acggtgacgc gcagcctggt ttccgctcat ctcgccctct acggtatgga
     9841 gctggcatca tgaccttact taacgtctgc ggcctttccc atcactatgc ccacggtgga
     9901 tttagcggaa aacatcaaca tcaggcggtg ctgaataacg tttccctgac cctgaaaagc
     9961 ggcgaaaccg tcgccctgct gggacgcagc ggctgcggaa aaagcaccct cgcgcggctg
    10021 ctggtaggct tagagtctcc ctcacaaggg aatattagct ggcacggcga atcgctggcg
    10081 aaacttaatc gcgcccaacg taaagccttc cgccgtgata ttcagatggt gtttcaggac
    10141 tccatcagtg ccgtgaatcc gcgcaaaacc gtgcgcgaga tcctgcgtga accgatgcgc
    10201 cacctgctat cactgaaaaa atccgaacaa ctggcgcgcg ccagcgaaat gctgaaggcg
    10261 gtcgatctcg atgacagcgt tctcgacaaa cgcccaccgc agttaagcgg cggccagctc
    10321 cagctcgtct gcctggcacg cgcgctggcg gtcgaaccga aattgctgat tctggatgaa
    10381 gccgtttcta accttgatct cgtgttacag gcgggcgtca ttcgcctgct gaaaaagcta
    10441 caacaacagt ttggcactgc ctgcctgttc atcactcatg acttacgcct ggtggaacgc
    10501 ttttgccagc gagtgatggt gatggacaac ggacaaatcg ccgaaaccca ggcggtggga
    10561 gataaattaa ccttttcctc tgacgccgga cgtgtgctac aaaacgcggt attacccgca
    10621 ttccccgtgc gccgtcgcac ctcagaaaag gtttaacgcc aatgcaacga gtcaccatca
    10681 cgcttgatga cgatttactg gagacgctgg acagcctgag ccagcgtcgt ggttataaca
    10741 accgttccga agctatccgc gatattctgc gtagtgccct ggcgcaggaa gctacccagc
    10801 agcacggcac acaaggtttc gcggtgctgt cgtatgtgta tgaacacgaa aaacgcgact
    10861 tagccagccg tattgtttcc acccagcatc atcaccacga cctctccgtc gccacgctac
    10921 atgtgcacat caaccacgac gactgtctgg aaatcgccgt gttgaaaggt gacatgggtg
    10981 acgtgcagca ttttgccgat gacgttatcg cccagcgcgg cgtgcggcac gggcatttgc
    11041 agtgcctgcc gaaggaagat tgagttattt ctaaaacaag gaaagaaaag atattaactg
    11101 aacttaattt ataaaattaa gttcagttgc atagatagat cctgtaaagg cagatattct
    11161 gcgtaatgta cgtcgctaaa atttataaat gattcaaacg ctcattcagt gtttcaataa
    11221 tataggtatt aacagagact tgttgctgcg cagccgcatt attaaggcgt tcactcagcg
    11281 attctggata gcgtaacgta aaggtcttaa tcttttctgt acgcgcatac ggctcaatac
    11341 ccgcagcttt acaatcctca agatactctc gcagggaaag ctctccttct ttttgcagcc
    11401 cctgaatact gtcagagaca aaatcacaat aacctgacaa gcccaggaat ttgccacgaa
    11461 atgcattcag ttctggcacg taggtaataa cagctggttg cccggcaatc tccatagagt
    11521 ttggcgtctt cagtttgatc atggttttac ccctatactt agtaaccagt cacacacgct
    11581 ttctacagcg cctttatcgg tatctggcga cggatgaggc cgatggaaac acgcaacgct
    11641 catatttagt atgaacttgc aacgcgaacc tcttccttcc ttaatttctc cgcctaatgc
    11701 tttaaccagt gactctatat cagaccactt gatcccttga ggaacaggag ttttaaatat
    11761 ttgatccaat gtattttttt gtttcttccg taaagcctgt accttggttc tcatggcatg
    11821 tccttatgac ttcactttat gacatcacag tatgaagtca agtttccctt ccttacgcca
    11881 ttgtcccaat cgtcttcctg aatcgcagca gtgcgatggt gaaaaacgcg ccgccaattg
    11941 ccatcagcgt caggaactgc ggccagacga tttcgaatcc ggcaccccgg tagaggatgg
    12001 cctgcgcgag gctaacaaag tgtgtcgtcg gcatggtcag cataatgtcc tgcaccatct
    12061 gcggcatact ttcgcgcggc gtggatccac cggaaagcat ttgcagcggc agcagcacca
    12121 gaatcaccag cagccccagt tgcggcattg aacgcgctat cgtccccata aaaatgccga
    12181 ttgacgtggt ggcaaacaga ctgagcgcca cgcccagcat aaacagcggg atcgagcctt
    12241 caatcggtac gcccagtaca cctttcacca tcagcaccag cgataatccc gataccacca
    12301 gcaccaccag ccccatcgac cagatcttcg ccatcatgat ctcaaacggc gttatcggca
    12361 tcaccagtaa atgttccacc gtgccgtgtt cacgctcgcg gatcagcgcc gatccggtca
    12421 atacaatcgc cagcatggta atgttgttga tgatcgccat caccccgcca aaccacgcgg
    12481 gatcaaggtt cgggttaaag cgcatccggg tttccagcga taccaacggt tcgctgttat
    12541 cacggtagcg cgcgacaaag ctgttcactt caccgttgat aatattctgg atatacccat
    12601 tgccggtaaa tgcctggctc atgcgcgtgg catcgacgtt cacctgaata tccggctggc
    12661 gtccggcgag gacatcacgc tgaaaattag gcggaatatt tatcgcgaag gtatagcgtc
    12721 cggcgtccag tccggcatcc atctcatcgg cggtgatcat ctccggtggc aaaaaccacg
    12781 gacgatagaa gctgttaacg atccggttcg ataactgcga ttgatccata tcggcaatgg
    12841 cgatcggcgc gaggttcaac gatcctggcg taacggtcgc tgacgaatac accgacaccg
    12901 taaacgagaa gacaatcagc gtcagcatcg ctttatcacc gagcagactg cgcaactctt
    12961 tgatacccag attaaaaata ttgcgtaaat ggcgcatcat ccctcctgtt ttttcagcag
    13021 caggatgctt aagcccatca ccagcgggat ggctatcagc aacgggataa aaagttgcca
    13081 caaatcagtc agatccagcg ctttcgagaa cgttccgcgg gcgatagtca gaaaatgact
    13141 ggtcgggtaa acctcgccga tccaacgtcc aggcccttcc agcgaagcta ccggatcgat
    13201 catcccggaa aactgtgtcg ccgggatcaa tgtgataatc gccgttccga aaatggcggc
    13261 aatctggctt ttcataaagg tggagatcag cagccccatt ccagtggcaa tgatgatata
    13321 cagcagcgcc gccaaggtga gcgtcaggaa actgccttta tgcggtacgc caaacacaaa
    13381 caccgacagg ccgcagagca ggaaaaagtt cagcatcccc agcgcgatgt acggcagctg
    13441 tttgccaagc aaaaattcgc tacgggtggt gggggtcacg taaaggttga taatcgaccc
    13501 tagctctttt tcccgcacca cgctaagggc gcttagcatt gacgggatca tcatcagcag
    13561 aagcgggatc accgccggaa caatcgctgg caggcttttt acgtccgggt tatagcgata
    13621 acgcgtctca atgttcatca gcccgctttg gctggcgggt gtcgattgtc ggctcgccac
    13681 atcctgaagc caactctggt gcattgcctg cacgtaacct tttaccgttt cggcacgact
    13741 gggcatcgct ccgtcgatcc agacgccgag ttccacaggc gtaccacgcg cgatatcgcg
    13801 cccgaaattg ggcgggatct caatggccac cgtgatatcg cccgcacgca tccgacgatc
    13861 aagctcgtca taactggtga gcggcggctg ttcgataaag taacgggaac cggagaggtt
    13921 gagtgtccac gcctggctac tgacggtctg gtcgcggtcg agcaccgcaa agcgcaggtt
    13981 ttccacatcc atactgatgc cgtaacccat tatcagcatc aggatcaccg ttcccatcag
    14041 cgccagcgtc gaacgtactg gatcgcgtcg cagttccagc gcttcgcggc ggctgtagct
    14101 aaacagacgg cgcaggctaa atccctgacg cggcgcgtgg gtggtgtcgt gtaccacggg
    14161 cggcgcttcg gcttcgttgc tctgccctgc cgcttcctgc aaataggcga taaatgcctc
    14221 ttccagactg gcggctccgc gtttctcaac cagttcctgc ggtgtaccgc tggcaagcac
    14281 ttttccggcg tgcatcagtg agatgcggtc gcaacgttcc gcttcgttca taaagtgggt
    14341 ggagatgaag atagtcactt tgtcctggcg cgagagatcg accatcaact gccagaacat
    14401 atccctcgcc accggatcga caccagaagt aggctcatcg aggattaaca tctccgggcg
    14461 atgaatcacc gccaccgcca gcgaaagccg ctggcgaatg ccgagcggca atgactccgg
    14521 cagaacatct tcaacgtcgt tgagcttaaa acgctcgctc atttcagcca ctcgcgcggg
    14581 aatttccgct tccgggatgt gaaacaaacg ggcatgtaac tccaggtttt gccgcacggt
    14641 gagttcgtta tagagcgaaa acgcctgcga catatagccc acacgacggc gggtatcgat
    14701 atcttttgga tcaaccggtt gcccaaatag ccacgcctca ccttcgctgg cgggcagcag
    14761 tccggtaagc attttcatgg tggtggattt accgcagccg ttcgaaccaa ggaatccaaa
    14821 aatctccccg cgtggaatgc ggaaattgac gtgatcaacg gcaacgaagg aaccaaaacg
    14881 catggtcaga tcgcgcgctt cgatggcaat ctctgcgttt tcaggttgat acggtgggat
    14941 cactaccgcc tgatgcgcct ggcgttgcgc ttgcggtaac agattaataa atgcttcttc
    15001 cagcgtggcg ctttgcgttt gctgccgcag ctcttcggcg ctgccggttg ccagcacttc
    15061 tccggcattc atcgctacca gccagtcgaa gcgttcggcc tcttccatat aggcggtggc
    15121 gaccagcacg ctcatattgc tctgccgctg gcgaatactg tcgatcagat cccagaactg
    15181 ggcgcgggag agcgggtcaa cccccgttgt tggctcatca aggatcaaca gttccgggtc
    15241 gtggattaac gcgcagcaca gcccaagttt ttgcttcatc ccgccggaga gtttacctgc
    15301 cggacgatcg cgaaacggtg ctaacccggt gctggtcagc agctcattaa ttcgcgcttc
    15361 ccgctccgct ttatcgtgac cgaacaggcg agcgaaaaaa tcgacatttt catacaccga
    15421 caaggtgtgg tagaggtttt tgcccagccc ctgcggcatc caggcgatgc gcgggcagac
    15481 gtctcggcga tgcttcgggt cgtgcatatc gccacccagc accaccacat tgccctgctc
    15541 aatgacgcgg gcaccggaaa tcagtgacaa taaactcgac ttcccgacgc catccggacc
    15601 aatcagcccg accatgcaac gggccggaat atcgagagtg atattgttca gcgcaacggt
    15661 ttttccataa tgctggctca cgcccgccag ttgcgcgaca ggcgggacgg gaaccagttc
    15721 cagatgcgtc attgcggcaa cctcaccacg aggtcgtcag gccacggaag ttcttcattc
    15781 acccgcaccc acgctacgcc cggtaaaccg gttttgacat attccagatg ctgctggagt
    15841 aattccggtg ggatacgcgc tttgacgcgg aacatcagtt tcagccgttc atcgctggtt
    15901 tcgacggttt ttggcgtgaa ctgggcgaca ctggcgacaa aactgatggt tgcaggaata
    15961 cgcagatctg gcgcggcatc gaggatcagc cgggcttcac cgcccagttt cagcgtgccc
    16021 gcctgttcgg ttggcaggaa gaaggtcata tagacgtcgc tgagatcgac catattcagc
    16081 acccgaccgc ctgccgccag cacttcccct ggctcggcaa cccgatactg cacgcgtccg
    16141 tcacgcgggg ctttcagttc actgtcatcg atatctgcgg caatgcgccg ttcagtggct
    16201 tgcgctgctt cgacccgagt ttgcgcctga atgatattgg tgcgtgctgc ttctatagcc
    16261 gctttagaag ccgaaacctg ggctttcgct gattccagcg cagctcgggc gctctcagcg
    16321 gcggcgcggt cgtcatccag ctgttgcgca gaaatagccc ctcgttgggc gagcgagcgg
    16381 gaacgcgtat gacgtttggc aacagagtcc agttcggcct ggcgttgatt gaccagcgac
    16441 tgtgcggcac gagtttcact ttgtcgttgc tccagcaaag cctgcgcggc ggcaacggcg
    16501 ctttgtgcct ctttgatttg cgcgatggct tccagtcgct gttcctgcaa cacgcgagta
    16561 tccatcttcg ccagcacttc gccttcgcga acaaactggc cttctttcac cagaatggtg
    16621 tcgatacgcc cggcaatttt gctggcgata tccacttccg tcgcttcaat gcgcccattg
    16681 ctgacagcaa agccttccgg cacacccgcc gggcgcaaca gccaccacgc cacgacggca
    16741 gccaccgcca gtgccccgac aacccaccac gccagatggc gcttactctt atccataatc
    16801 gacccgccat aatccctgta agcaaacgac gcgtgttaca actgccgccg aacgacgttc
    16861 agcggattct tccatccggg ataagaagga aacgacaaat acgaccagcg cgcagagcat
    16921 tgagcgccaa cggggaaggc ttagtgaaaa cgggtagtaa tgttgtcatc atcagcattc
    16981 ctggccgtaa atgaaacacg catactcata agtgtgatct ataaaactgg ctgacaaaac
    17041 ggtatccatc ctgttggatc gcatttcacg cctgttcagt tgcaggaaca gagcattcct
    17101 ttttacgttt tatcaacagt ctcaaccaga tagtaacatt aatattcatg ttgtctatgg
    17161 ttcagagctg taacattgtg cgccttctaa tcaaaggcat aagtccattt ctgttttttt
    17221 catgctggta agacttccag ggagtggtta tctctggccc tgcaccaggg ttaatttggc
    17281 aattatgcat gctgaacatt cttccagcat gtttactgtc acgggcgtta agacctaaaa
    17341 ctgtccccgt ttcacctatg cgttctctcc caccatttcc gttatgtgca acaacttgat
    17401 ttatacctaa catttgtgaa catgctaata gtgcttcaag tttaggtctg taatctgtcg
    17461 aaacagaaaa ataacgatta aaggatcgac aagagatagc ccggctagtt ttaaactttt
    17521 tgccattaat tatagcatga tgctttttat taataatttc cgctaattcg accggatcga
    17581 gagcattggt catttcatca atagagacag ttatcggtcc tataatgtaa cgttttcgct
    17641 gatcatcaaa agttatccca tgatgatttg ccattatttt gtatttgggg tcatagaaac
    17701 atacaggata atcttttatc ccgtcgtagg tatcctcatc aagtggtttc atgcgcgtga
    17761 agttttgcat atagttacca ttgcaattcg tttcatgatt tccagctaat acatgaacat
    17821 tttcattgat actcattatg cgttttaaca aatcgatgat aaattgatcg ccacagatat
    17881 tactaaatcg atcgccggtt tggtcgccaa gaaacgtaca tggcgtgact gcgtctttat
    17941 taataaccaa ataaggctga agactttcta aaacattatc tcttctgttc agtatactga
    18001 ttattgtatt aacattagtg tcaagttgct cattaaagtt ctcccttagc aatacgttga
    18061 gaacttcatc ttctatatta agcaactggc taaggaaatt ttctccttca gccatgattc
    18121 ccaaatgtcc tgttgcaaaa agcgcgtaca aaactgcacc gactgagcca tcggtatcgc
    18181 caaaataggt cggcctggta cttgtgggat caacaggtga acaattggag aaataggttc
    18241 tgtcagcgaa ctggggtttt ataattgttt cgaatttaat atcaaataca ttgtattgcg
    18301 atatttccca gttccagatg ggtttgtacg actcagtaac tgcaacatca ttagcattat
    18361 catttactgt catattatta agtaaataaa caatgctgca ccaattaata tcggtattat
    18421 caagagggca accgtaattt ttactattaa ttgtcacaat aaactgataa cagttattcc
    18481 tacgtagaat atgagcgcgc atgcccgata gcatactata tttttctgaa tgattcgttt
    18541 gtatggtaaa tgaaattttg tcatcttgaa tagattgttg gaaacgttct atttgccaat
    18601 catcaatttt ttccacaact tcaccgagca ttgtcctggc aatacttgat atatcatcac
    18661 ctataccatt tgcaatatac ccaaacagtt ctttagtgaa gttggtaaca tgctcttgca
    18721 ccatatcatt tgttgcgatt gtcgttgtac tgccattagt gcctgccaca gttccaattt
    18781 tcatctttta cctcatatta ttcaccgcga ggaatacata gttaacattt tatcttatga
    18841 catacacctc atattcgatg gggaaataat tatattttgc acaaatcatt cagccttaaa
    18901 gcataatatg aaatttctta tctcatagca gagaaagtcc ctaaatattg tagaggcgtc
    18961 atcagtacat acctctacaa tcgattaaga aaacctatga aatactggca aaattaaaaa
    19021 gagataccac agcactcatg aagttttatt gaggttgaaa acaactaagc cctgggacac
    19081 cttttttatc accacgttcg ttaggtgcta tcttccaaga atttagcccc atggtataat
    19141 tatcaatttt ttgatgctgg tctaccccta atccataatg gccaaatttc tgtttagttt
    19201 ttttgagcgt ctgtccacat ttgaagatat cttcaggttt tggtctgaaa gcagtagtgt
    19261 tattaaaata attttcatat acaggtgtgg gtgctatagc attgcttcta attagatgga
    19321 gaagatgatc atccatgtgt tgtttatgtt ttttattaca tatatttgct aattcctcag
    19381 gatcaattgg gtctttcatc tgattaaatg gaacctgcaa agcaccgaga caataacatt
    19441 gattttcttc atttcttatt attccgtgat gactggttaa aactttagtt tctgagttat
    19501 aattacaaac atcgagcgtt ttaattaagt tatacgtatc accggcagag agtttataat
    19561 tttctagtcg tgcagtatag ttaccattaa aattgatttc atgatttcca gccagaacga
    19621 tgacattttt attaatacgg ctatctttat taccctccat atttcgcata gaatttagca
    19681 aagtaaggat atatttatca ccaaaaatag tactaaatct atcgcctgta tgatccccaa
    19741 ggaagataca aggggttatc gcatcgtcct ttaaaaaaac atgttgatgc aactcattca
    19801 atatcatgtc tctcgtattt aataaattgt agagcaattt attattttta tgtatgtatg
    19861 cagatgaagc catgtcttcg tgttttaata attcacataa taaactccaa cccttttccc
    19921 tgatgcctat atgccctgaa acgagcaatg aataaagtac agcgcctact cttccatctg
    19981 tatcgccaaa ataagccgtg ttggcatttt gtgggttaat cacaccacat tgtttataaa
    20041 agtctatttt agaaaacaca gatttaacat agctatcaaa cttatcctta ggaaacgtct
    20101 ctttagagac atcccaacgc cagtcagctt taaaattacc gctaagaaga gtaaagaaat
    20161 tttcaggtat gatagaaagc ccattaactt tataaattat gtctgattta tctccggaga
    20221 aattaataaa ataataacca tccttacact caatcttaac tgtaatggga gacgataatt
    20281 ttatcacatg gttattgtct ggtataattg taaagataaa cgtgttagta gtattatcgg
    20341 caaaactatt atattcttga atatctatag atggctcact attgtaaaac catattttct
    20401 ccatctgaag actatgttca tttaccctcc agtgaccatg ttgcaggaaa tagatgatat
    20461 gcatgccaaa atcgttatct atttgcgaat cattatccag cttatcaatt tcaactatct
    20521 ttgaacgatg tgttttatca accttgctat ggtgttgatg attattaact ttatctgaat
    20581 gcttatcttt atggtttgaa gggatgtact taaatatatt gtcaattgct ttcataaata
    20641 acccgcgata tttttgacct gttagtaaaa aaataaatga tacatgtctg aaagacatat
    20701 ttacgacgat ataaaacaat aatacttata tgttcgctga ttttaagcac cgagactatc
    20761 cggtctttta ctaaataatg gaaaacaatc catccctgga agacaattgt aaaaaaaaac
    20821 aacagaataa gaattaatct taggataaat atttatttat catggccttt tgaccgctaa
    20881 aaaatggcac tcggcacagt aaaccccgct atagttatgc tatgcatttg tctttaatga
    20941 tatctcgtcg ttatattaac aggctgaaat tatcattaat gcacatttca atattagcag
    21001 ggatacctga ggagtttaaa gtgaacatat atatcgggta gcttttcaaa ttaatccctt
    21061 tgattatagg cttaatttgc atcgcgttag gtggctttgt gctggaaagt tcagggcaac
    21121 gtcacgggtc atgtgctgat ttctctggcg gccatatgcc tggcattatt cactaccgca
    21181 tttattatca tttcgcaact cacgcgcggc gttaatacgt tttacaatac attgttcccc
    21241 attattggct atgcggggtc aattatcacc atgatatggg gttgggcact gttagcaggt
    21301 aatgatgtga tggcagacga gtttgttgcc ggccatgtta tttttggcgt tggtatgatt
    21361 gccgcatgtg tatcgacggt ggcagcgtcg tccggtcact ttctgctcat tcccaaaaat
    21421 gcagcgggga gcaagagcga cggaacaccg gtacaggctt attcttcatt aatcggtaat
    21481 tgcctcattg ccgttcccgt tttactcacc ctgctcggtt tcatttggtc tattacgctg
    21541 ttacatagtg ccgacataac tccgcattat gtcgccttgg gttaaccgca atctgtgcct
    21601 gtctaattgg cctagtggcc acaatcgtcc atcaaacacg taatacgttc tcatccaaag
    21661 agcactggct gtggtgttat tgggttattt ttctcggctc agtcacggtg ctgcagggga
    21721 tatacgtctt agtcagttcc gatgcaagcg cccgactggc tcccggcatt attctcattt
    21781 gcctcggaat gatctgttac agcatattct caaaagtctg gctactggca ctggtctgga
    21841 gacgtacctg ttcgttagcc aacagaatac cgatgatacc ggtatttacc tgtctgtttt
    21901 gcttgttttt gtcatcattc ctcgcggaaa tcgcacaaac tgagctggac tactttattc
    21961 catctcgggt tctggttgga ttaggcgcag tatgttttac attgttctct attgtttcaa
    22021 tattagaggc cggttctgcg aaaaaaaatt aaaatttaca taatcccagc gttattcata
    22081 tcttaacgct ggttttttac gaacttagtg aaataatgta gaatataaaa ttataatttt
    22141 atattggagc acatgtgtta atgatcaacc ccgggatact aaaaatatag acattattaa
    22201 acttgcttag cctcgcattg cctttacctg ttaaagatgc atcgagaaaa ttgagtctga
    22261 agttctcatc ctttatctga ttttccagtc ccgaaacttt ggccttagca ttacctgaga
    22321 tccgtccgtt actatctatt ttgtaggtaa aaaaatacga atgggaaaat ttcctcggtg
    22381 tatcttcatc agttaagcca acaatcgtaa aactaccgct accatctttt tttaaaatga
    22441 ctgaaaggtt agcagagtag tataaagaat gatctgtttt agcatttctt gttaacgtcg
    22501 acgtacattc aagagacgtt gtgctaccat cacgagcctt ccagagagca aaaaaacaca
    22561 cctgtagcaa tgtgaaaaca agtattaata taaaatacat tttcttatta gtcatataat
    22621 agcacctctg aacaaattgc ataagtttcg tcattagagg attttataca tcttgataaa
    22681 taccgctgcc cttccccaag caaataatgc atactattta catcatatat aaaatttgca
    22741 cctggcacac agggtaaatg ctttttcgaa atttcagcgg cctctatttg agatttagcg
    22801 aggccaatct cgttaagatt ctctgaaaga tataaatcac aggtgtcaat cttacctgat
    22861 ttaaccaatg gataatccgt tatactgtag gtatctttaa aagccacggc aaacatttca
    22921 ataataacaa ttactaccac aagcgaaatc aaaatgcgaa aaagaggaat actccttttg
    22981 cgctctactt tttcaacaac atcttcttgt aaatgagaat catcataaga ttgtttactt
    23041 attgttatgt tttcactgat gtaaaagcct actttgggta tcgtaagaat cacattttct
    23101 tcaacaccaa tatttttaaa acttttgcgt aacagggata tatattggtt caatgtgttg
    23161 tttgacagat caaatgaaaa tctctccaaa acgctatcaa tgattgagtt cctgtcaacc
    23221 gctccaccat tactttccag taacactgcc aaaatacacc ccgccgtagc tggaaaattt
    23281 atctcatcct gggtatttac attcgagagc gagcatttgt tcggatcaaa aataatcgcg
    23341 tcattgataa tgaaaaaaca tctccgctcc atgcaggcaa caacacaaaa taagattttg
    23401 tcattttagc ctattcctgg tggaactgac acaagaaact aaaatacaaa tataaccctg
    23461 aaaaagtcat ataaaactta tatccacaga ataaaaaatc aaacatgtca gttttatcaa
    23521 tataaagtat cgcaaggcga aaagtgatgc aagaatactc ctaagtaaat gaaagcgcac
    23581 cacatgaaat tatcaacaca tgtaatttca tcattaggtg ctatcgaaaa taactcagga
    23641 gctacttaag atgaataatg tcttactcat gagacccgat gactcagaat ttatgatacc
    23701 tgatgactgg tcagggctaa acgcaaaagg cattacatac gaacaagata atagagtgac
    23761 agagtctttt ctgcctttta aatcttttta ttgttcaccg gtgtgcacga cagttgaact
    23821 catctcacaa aataattgtt ttgctatggg tatccgctac cttctggaaa agaaaagaga
    23881 cttaaggcta aatcacttta aagccttctc tcataaatgc gagacaaaac ctgacgtgtt
    23941 aattatgacc gggtgcaccg tcgctgatac ttatagtata ttctcacgca ttgttgaaga
    24001 aaatataatg aaaacgatga cacctatcgt catcattact gggcatcact cagaagatct
    24061 ttgttctctg gcatcgaaac tgggaggagt taaaataata tcaccgacag aaaagattgc
    24121 aaaaatagaa aacgctatca atgatgcttt taacagcaag cctaaaaaaa taagattttt
    24181 aacagatata ctctctcctc aacaaattga gaccatgaaa atggttgcgc agggtagatc
    24241 cgttaatgag atttcagccg caatgaatat tagcatcaag accgtgtatg cacataaagt
    24301 tcgtgctatc aataagctgg gaattaagaa aaaagtttat gaagcgcttt tttataaagc
    24361 gctaattcag ttcaactgat gtaaaaatca ccctcgctta ataaataata tattctatgt
    24421 ctttttttat aagttaaaaa aggcgggata tcaaatcccg ccatgaaatt aactaatcat
    24481 ctgcattatt ctgcgtagat ataagcctgg gtacggatgg tgtaagagtc tggctcaaca
    24541 acctgttctt tcatatggga aacgacgcga taggcaccac ctgttccagt atttttcagc
    24601 atcatataac gcttaccgcc aatctcttcg gaggcagctg aaggaggatc ctgctcaagc
    24661 gttgtcaggg aaactttcag tttatggtcc gggttattgt caccagaaat tactgcatcc
    24721 gctgaagtac tgctctccgc ataaatatca tccgggaaag ttacagcata gtcgtggctc
    24781 tgcccatcag atgcgccaac aatacctgtt gccaaaggcg tccatgcagt taacgtaccg
    24841 ctatataccc ctttcactgg ttgaatggta ttattaacaa cgataggctt ggtcaaagta
    24901 agattagctg acgcttcagt accctggctt actaaagttc ccgcctgaga agcaaaagct
    24961 gtccccgcca gcatgattgc taacaatgtt ttcttcatta tattttctcc atgattttca
    25021 tttacactgc aaaatgcagt ttctttcttt gcatttacgt gctatcgcga aaatgcttcc
    25081 accaaataat ggaaacatct tcacaaaaat aaattatgag tttcttaatt tagaaactat
    25141 aaacatgtcg ccgctatctg gtataaccct gatgcacttt tcttcgcagg aagttggtat
    25201 tttgcgatac aggattgatc tttagaataa ccccatttca cattaaactc gccttccgca
    25261 ctcaagccag aaacataagc aataccatct tcacctacaa tgccggccat attttcagtg
    25321 tctttgctat aactaatcac agcgccgaag gggatgggtt taccctcttt ctgttttaag
    25381 gttactaatc ctttatcccc gctcttcact gccaatgtcg caggcacaac ggctccagct
    25441 gtcggtacaa ccaattgact agtttgtggt aagtcgtaat tcttacctat cgttttttca
    25501 tccagaataa catcgtctct tcggtatggc tgcactggca gtattgcata cccacgaaaa
    25561 tcagtactca gtcccggcca gccattaacg ttaacccccg ttgctccggg tgcctctata
    25621 agggctaatg tcccatccac attttgacca aatgtaagtc catcccttgt aataagtatc
    25681 ccaccgctta aatttgcacc tagctgtttc atgttttcgc tggagctgta atggctacta
    25741 aactgaccat atgatccatt tttgccaact tgcacaatgt tcatcgtgct cttcggttgt
    25801 gactgatgag cgttagttat atttgtcgct acatcccacc atacaccacc actattctga
    25861 cctgataatg aaacctgata gttattaccc tgctctcccg aacgactcat ctgtgaagac
    25921 gcataaattt gattttcgtc acccagcgat aatggcactg aaatacccac gcttaatttc
    25981 cgatctactt tccatttatg cgatgtcagc aagtcgtttt ttgcccagtt aatggagaat
    26041 gttatgcctc tccataccgt actataactc aggctggtag accgcgtgtt tcctgtgccc
    26101 cgggaagaat tatacctcgt ggtattgaag ttcaaattcc cgatattacc aaggggctgg
    26161 gataaggata tcgtagagcc atcgtgacta ttcttatctc tttttctgtt ccgttcatcc
    26221 tgtgcactta ctgcttcagt cagtgtggca taacctgatg cataacggtt cgtggttata
    26281 ga
//
LOCUS       DADLTY010000043        25833 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.043, whole genome shotgun sequence.
ACCESSION   DADLTY010000043 DADLTY010000000
VERSION     DADLTY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.043"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="JN334_001512"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="JN334_001512"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HAY7437695.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="JN334_001513"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="JN334_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HAY7437696.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="JN334_001514"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="JN334_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY7437697.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPSIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="JN334_001515"
     CDS             3807..3995
                     /locus_tag="JN334_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437698.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="JN334_001516"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="JN334_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HAY7437699.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..5115
                     /locus_tag="JN334_001517"
     CDS             4756..5115
                     /locus_tag="JN334_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HAY7437700.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5112..7373
                     /gene="bcsA"
                     /locus_tag="JN334_001518"
                     /pseudo
     CDS             5112..7373
                     /gene="bcsA"
                     /locus_tag="JN334_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7384..9723
                     /gene="bcsB"
                     /locus_tag="JN334_001519"
     CDS             7384..9723
                     /gene="bcsB"
                     /locus_tag="JN334_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAY7437701.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9730..10836
                     /gene="bcsZ"
                     /locus_tag="JN334_001520"
     CDS             9730..10836
                     /gene="bcsZ"
                     /locus_tag="JN334_001520"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HAY7437702.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10913..11254
                     /locus_tag="JN334_001521"
     CDS             10913..11254
                     /locus_tag="JN334_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HAY7437703.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11169..11420
                     /locus_tag="JN334_001522"
                     /pseudo
     CDS             11169..11420
                     /locus_tag="JN334_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11502..13490
                     /gene="hmsP"
                     /locus_tag="JN334_001523"
     CDS             11502..13490
                     /gene="hmsP"
                     /locus_tag="JN334_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HAY7437704.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            13679..14965
                     /locus_tag="JN334_001524"
     CDS             13679..14965
                     /locus_tag="JN334_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAY7437705.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15186..16682
                     /locus_tag="JN334_001525"
     CDS             15186..16682
                     /locus_tag="JN334_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY7437706.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(16778..17926)
                     /locus_tag="JN334_001526"
     CDS             complement(16778..17926)
                     /locus_tag="JN334_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7437707.1"
                     /translation="MFKSDAARTRQRLSARKGRTLPPGRGKFPHSTTESFRNTFWLKK
                     IMEHCFNMVDQQTTTAQTNANFLQIRFTTMSKKIAVIGECMIELSEKGADVKRGFGGD
                     TLNTSVYIARQVDPAALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGL
                     YYIETDSTGERTFYYWRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTS
                     REKLLSLLRECRAKGGKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDA
                     LWGQQPVEDVIARTHNAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTA
                     AGDSFSAGYLAVRLTGGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17939..18706
                     /gene="pdeH"
                     /locus_tag="JN334_001527"
     CDS             17939..18706
                     /gene="pdeH"
                     /locus_tag="JN334_001527"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HAY7437708.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18776..20836
                     /locus_tag="JN334_001528"
     CDS             18776..20836
                     /locus_tag="JN334_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY7437709.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21018..22340)
                     /locus_tag="JN334_001529"
     CDS             complement(21018..22340)
                     /locus_tag="JN334_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAY7437710.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22751..23764)
                     /gene="yhjD"
                     /locus_tag="JN334_001530"
     CDS             complement(22751..23764)
                     /gene="yhjD"
                     /locus_tag="JN334_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HAY7437711.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23813..24784)
                     /locus_tag="JN334_001531"
     CDS             complement(23813..24784)
                     /locus_tag="JN334_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7437712.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSE
                     LDGLFQQDATSISGKLRIDIPSGIAKNLLLPRLSEFLYQHPGIELELSSHNRPVDILH
                     DGFDCVIRTGALPEDGVIARPLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSSENYLAAGLAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEHLD"
     gene            25232..>25833
                     /locus_tag="JN334_001532"
     CDS             25232..>25833
                     /locus_tag="JN334_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7437713.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         6156 a         6489 c         7030 g         6158 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tactcggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tattttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaatggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgagc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt
     5101 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catctggatg
     5161 aaaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
     5221 tcaccttctc gctgattctg gcgttaatct gcgttactca gccgtttaac ccgctggcgc
     5281 agtttatctt cctgatgctg ctgtgggggg tagcgctgat cgtacggcgg atgccggggc
     5341 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
     5401 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
     5461 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
     5521 ggccgctgaa tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg
     5581 atatctttgt cccgacttac aacgaagatc tcaatgtggt gaaaaatacc atttacgcct
     5641 cgctgggtat cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca
     5701 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc
     5761 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
     5821 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
     5881 ggttcctgaa agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg
     5941 acccgtttga acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct
     6001 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
     6061 cggtgattcg tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg
     6121 aagatgcgca tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta
     6181 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
     6241 gctgggcgcg cgggatggta taaatcttcc gtctcgataa cccgctcacc ggtaaaggac
     6301 tgaagtttgc tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
     6361 cacggctgat cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct
     6421 atgcgccagc gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga
     6481 ccaactcgaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
     6541 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
     6601 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
     6661 cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc
     6721 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt
     6781 tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac
     6841 gccgatcgaa ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
     6901 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
     6961 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
     7021 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
     7081 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
     7141 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
     7201 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
     7261 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
     7321 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
     7381 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
     7441 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
     7501 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgtcaggcga gcagggagct
     7561 gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
     7621 atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg cagcattgag
     7681 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacttcga atacacgcca
     7741 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
     7801 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
     7861 aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
     7921 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
     7981 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
     8041 ttctttgacc cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg
     8101 gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc
     8161 tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc
     8221 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg
     8281 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
     8341 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
     8401 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
     8461 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
     8521 gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct aaacctgccg
     8581 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
     8641 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
     8701 tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat tccggtatta
     8761 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
     8821 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
     8881 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
     8941 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
     9001 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
     9061 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
     9121 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
     9181 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
     9241 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
     9301 attgtgccgg acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt
     9361 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
     9421 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
     9481 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
     9541 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
     9601 ctggcaaacc atccgattct gctggcggtg ctggcgacta tcagtgtgat attgctggca
     9661 tgggtactgt ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag
     9721 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt
     9781 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
     9841 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
     9901 agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga taatattctc
     9961 gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc cgcctggctg
    10021 tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt
    10081 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    10141 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    10201 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    10261 tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    10321 tggaccacgc tgcgcgaaac caatcaacat ttattgctgg aaaccgcccc gaaaggcttt
    10381 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    10441 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    10501 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    10561 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    10621 gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag
    10681 cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta tgtgctgacc
    10741 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    10801 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    10861 tggccgtcat gccgatggtc gaggcagcac caaccatcag cagaccctgc ggatggacag
    10921 cggcaacact aatgccgtgc gcgggctggc gaatatttac cgccagcagt cgccagaaaa
    10981 agctgaagcg tttatcgcct cgctctctgc cagccagcgg cgcagcattg atgatatcga
    11041 acgcagcctg caaaacgacc gtctggcaca gcaggcagag gtactggaaa accagggcaa
    11101 atgggcgcag gcggcagcac ttcagcggca acgactggcg ctgggccccg gcagcgtatg
    11161 gattacttac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    11221 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    11281 ttggcacggc aattgatatc cagcaggtga aagattacgc acccagccat ttcctgctct
    11341 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    11401 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    11461 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    11521 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    11581 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    11641 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    11701 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11761 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgtccggtgc
    11821 cggtaatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtctactcgc
    11881 tcgaacgtcc ggcaaatccg cagccaatag cctatctggt gctacaggcg gattccttcc
    11941 gtatgtataa gttcgtgatg agcactctct caacgttagt gaccatttac ttacttttgt
    12001 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    12061 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    12121 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    12181 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    12241 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    12301 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    12361 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    12421 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    12481 gtgtacagga accgtggcac gcaatcactt tgggtcagca agtactcact atcatgagcg
    12541 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    12601 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    12661 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagcagccc
    12721 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcaa
    12781 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12841 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12901 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12961 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    13021 aactgatgca cccgaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    13081 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    13141 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    13201 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    13261 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    13321 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    13381 cacaacgaga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    13441 gcccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    13501 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    13561 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    13621 cgcaggtacc ccccccccat aaccttacaa gacctgtggt tttactaaag gacaccctat
    13681 gaaaacctct ctgtttaaaa gcctttactt tcaggtcctg acagcgatag ccattggtat
    13741 tctccttggc catttctatc ctgaaatagg cgagcaaatg aaaccgcttg gcgacggctt
    13801 cgttaagctc attaagatga tcatcgctcc tgtcatcttt tgtaccgtcg taacgggcat
    13861 tgcgggcatg gaaagcatga aggcggtcgg tcgtaccggc gcagtcgcac tgctttactt
    13921 tgaaattgtc agtaccatcg cgctgattat tggtcttatc atcgttaacg tcgtgcagcc
    13981 tggtgccgga atgaacgtcg atccggcaac gcttgatgcg aaagcggtag cggtttacgc
    14041 cgatcaggcg aaagaccagg gcattgtcgc cttcattatg gatgtcatcc cggcgagcgt
    14101 cattggcgca tttgccagcg gtaacattct gcaggtgctg ctgtttgccg tactgtttgg
    14161 ttttgcgctc caccgtctgg gcagcaaagg ccaactgatt tttaacgtga tcgaaagttt
    14221 ctcgcaggtc atcttcggca tcatcaatat gatcatgcgt ctggcaccta ttggtgcgct
    14281 cggggcaatg gcgtttacca tcggtaaata cggcgtcggc acactggtgc aactggggca
    14341 gctgattatc tgtttctaca ttacctgtat cctgtttgtg gtgctggtat tgggttcaat
    14401 cgctaaagcg actggtttca gtatcttcaa atttatccgc tacatccgtg aagaactgct
    14461 gattgtactg gggacttcat cttccgagtc ggcgctgccg cgtatgctcg acaagatgga
    14521 gaaactcggc tgccgtaaat cggtggtggg gctggtcatc ccgacaggct actcgtttaa
    14581 ccttgatggc acatcgatat acctgacaat ggcggcggtg tttatcgccc aggccactaa
    14641 cagtcagatg gatatcgtcc accaaatcac gctgttaatc gtgttgctgc tttcttctaa
    14701 aggggcggca ggggtaacgg gtagtggctt tatcgtgctg gcggcgacgc tctctgcggt
    14761 gggccatttg ccggtagcgg gtctggcgct gatcctcggt atcgaccgct ttatgtcaga
    14821 agctcgtgcg ctaactaacc tggtcggtaa cggcgtagcg accattgtcg ttgctaagtg
    14881 ggtgaaagaa ctggaccaca aaaaactgga cgatgtgctg aataatcgtg cgccggatgg
    14941 caaaacgcac gaattatcct cttaatctca ccacttatgc ccgcactccc ttccccgagt
    15001 gcgggtgttt gcgcataatt gacccctgtg cctgaactta ccacctgggt gagttcaggt
    15061 ttatttcact tcttccgtga cattttcatg ttcttgcggt ctaacacgaa gtgtttttaa
    15121 cgtcatttag actgaccgac aacgggcagt ttttttatta ccaggatagt tgatcagggg
    15181 ttcacatgca gggcataaaa attcgacttt tagcgggcgg tttgctgatg atggccactg
    15241 ctggctatgt gcaggcagat gcgctccagc ctgatccagc atggcaacag gggacgcttt
    15301 ccaacggttt acagtggcaa gtgctgacca ccccccagcg tcccagcgat cgtgttgaaa
    15361 ttcgcctgct ggttaatacc ggttcgctcg ccgaaagtac acaacagagc ggttacagtc
    15421 acgccatccc tcgtattgcg ctaacgcaaa gcggtggcct tgacgcagca caggcgcgtt
    15481 cattgtggca gcaggggatc gaccctaaac gcccgatgcc gccggtaatt gtctcttatg
    15541 acaccacgct gtttaatctg agtttgccca ataaccgtaa cgatttgctg aaagaagcgc
    15601 tctcttatct ggcaaatgcc actggcaaat tgaccatcac accagaaacc atcaaccacg
    15661 cgctgcaaag tcaggacatg gtggcaacct ggcctgccga tactaaagag ggctggtggc
    15721 gctatcgtct gaaagggtca accttgttag gtcacgatcc tgccgatccg ctgaaacaac
    15781 ccgttgaagc ggaaaaaatt aaagatttct atcagaaatg gtacaccccg gatgcaatga
    15841 cgctactggt ggtgggaaac gtggatgcgc gctcggttgt cgaccaaatc aacaaaacgt
    15901 ttggcgaact gaaaggcaaa cgtgaaacgc cagctccggt gccgacgctt tctccgctgc
    15961 gtgcggaagc ggtgagtatt atgactgacg cggtgcgtca ggaccggtta tctatcatgt
    16021 gggatacgcc gtggcagccg attcgtgaat cagccgcact gctgcgctac tggcgtgcgg
    16081 acctggcccg cgaggcgctg ttctggcatg ttcagcaagc gttaagcgcc agtgacagca
    16141 aagacatcgg tcttggattt gactgccgtg tgctgtatct gcgtgcgcag tgtgccatca
    16201 acatcgaatc accaaacgac aagctgaaca gcaaccttaa tctggtggcg cgtgaactgg
    16261 cgaaggttcg cgataaaggt ctgccggaag aagagttcaa tgcgttagtg gcgcaaaaga
    16321 aactggagct gcagaaactg tttgccgcct atgcacgggc tgataccgat attctgatgg
    16381 gccagcggat gcgttcgttg caaaatcagg tcgtcgatat cgcgccggag cagtatcaga
    16441 aactgcggca ggatttcctt aacagcctga ctgtggagat gttaaatcag gatctgcgtc
    16501 agcagttgtc gaatgatatg gcgttaatac tgctgcagcc gaaaggcgag ccggaattta
    16561 acatgaaagc gttgcaggcg gcctgggatc aaatcatggc cccatctacc gccgctgcga
    16621 ccacctctgt cgccacggat gacgtacatc ctgaagtgac ggatattcca cctgcacagt
    16681 aatgcctgat ggtgatgagt tagcacaacc atcaggtatt tatggatgag ctggatagag
    16741 gggtaacact ttatccagcc ttttgcatat gctgcgttta cgctggcatc gcctcacgcg
    16801 ggataatcgc gccgcgatac tgaataacgg tacttgcggt caggtgcccg cgtttcgccg
    16861 cgttttccgc actgccgccc gtcaaacgta ccgccagata accggcgctg aaagagtcgc
    16921 cagctgcggt ggtatcgatc actttttctt tcggcagttt caccgccgga acatcaacta
    16981 acgcttcacc agcaatggac accaggcaag aatccgcccc gcgtttcacc accacttctt
    17041 tcacgcccgc gttatgggtg cgcgcaatga cgtcttccac cggctgttga ccccacagcg
    17101 cgtcttcgtc gtccagcgtc aggaaggcga tatccgtgca ttccagcatt tgctggtaca
    17161 cctgctgtgt ctcttctttg ctggcccaca ggcgcggacg atagttattg tcgaaaatca
    17221 cttttccgcc tttggcgcgg cattcgcgca gcagggaaag cagcttttcg cggctggtcg
    17281 ggcttaagat cgccaggcta atcccgctca ggtagagata atcgaaattc gccagctctt
    17341 cgcaaatcgc cgcagactgc tcgctcgcca gccagaattt ggcggcggct tcgttacgcc
    17401 agtagtagaa cgtacgttcg ccggtgctgt cggtttcaat gtagtaaagg cccggaagac
    17461 ggttttccat ccgttgggtc agggaagtat caacgttctc gccgtgccag gcgtccagca
    17521 tctgctggct aaaactgtcc gttcccagcg ccgttacgta atgaacggtt aatgccgcag
    17581 gatcgacctg acgggcgata tagacggaag tgttcagggt atcgccgccg aaaccgcgct
    17641 taacgtccgc gcctttctcg gaaagctcaa tcatgcattc gccaatcacg gcaatctttt
    17701 tggacatagt cgtgaacctg atctgtaaaa aattagcgtt agtttgcgct gtggtggttt
    17761 gctggtcaac catattaaaa cagtgttcca ttattttttt gagccagaac gtattcctga
    17821 aagattccgt tgtggagtga ggaaatttac cccggccagg cggtaaagtt ctgcccttac
    17881 gcgccgataa tctttgtcga gtccgggcag catcactttt aaacacagga catctttgat
    17941 gataaggcag gttatccagc gaataagcaa ccctgaagca agcatcgaga gcttgcagga
    18001 acggcgtttt tggttgcagt gtgagcgtgc ttacacctgg cagccgatct atcaaacatg
    18061 cgggcggtta atggccgtgg agctattaac ggtggtcacg catcccttga acccttcgca
    18121 acgcctgccg ccggatcgct attttactga aatcaccgtc agccatcgga tggaggttgt
    18181 gaaagagcag attgatttgc tggcgcaaaa agccgacttc tttgtagagc acggcctgct
    18241 ggcatcggtc aatattgatg gccctacgct catcgccctg cgtcagcaac caaaaatcct
    18301 gcgccagatt gagcgtcttc cctggctgcg tttcgaactg gtggagcata tccgtctgcc
    18361 gaaagattca acctttgcct cgatgtgtga atttggcccg ctgtggctgg atgattttgg
    18421 taccgggatg gcaaatttct ctgcactaag tgaagtgcgt tatgactaca tcaaaatcgc
    18481 gcgagaactg tttgtgatgc tgcgtcagtc gccggaaggc cgcacactct tttctcagct
    18541 tttacatcta atgaatcgct attgtcgcgg ggtgattgtc gagggcgtag aaacaccgga
    18601 agagtggcgt gatgttcaga actcgcccgc attcgccgca caaggctggt ttctttcacg
    18661 cccggcaccg atggaaacgc tgaatacggc ggttctggcg ctataagctg cctcattttc
    18721 cgccgggctg gactatcttt aggactggca caggaagaat atgaggaagc gaacgatgag
    18781 caaggcaggc aaaataaccg ctgcgatttc aggggctttc ttgttgttga ttgtcgtggc
    18841 gatcattttg attgcaactt ttgactggaa tcgactcaaa ccgaccatca accagaaagt
    18901 ctctgcggag ttgaatcgtc cgttcgctat ccgtggcaat ctgggcgtgg tgtgggagcg
    18961 gcaaaaacaa gaaactggct ggcgcagctg ggtgccgtgg cctcatgtac atgcagaaga
    19021 catcattctt ggtaatccac cggatattcc cgaagtcacg atggtgcatt tgccgcgagt
    19081 agaggcaacg ctggcaccgc tggcgctgct gaccaaaacg gtctggctgc cgtggatcaa
    19141 gctcgaaaag cccgacgcgc gcctgattcg cctctctgaa aagaacaata actggacgtt
    19201 taatcttgcc aacgatgaca acaaagacgc gaatgcaaag ccgtcggcat ggtcgtttcg
    19261 gctggataat attcttttcg atcaagggcg gatcgccatt gatgacaaag taagcaaagc
    19321 ggatctggaa atttttgttg atccgttagg caagccgctg ccattcagcg aagttactgg
    19381 atcgaaaggt aaagcggata aagaaaaggt gggcgattac atttttggcc tgaaggcgca
    19441 gggacgttat aacggtgaac cgctcacggg tacgggaaaa ataggcggta tgctggcgct
    19501 gcgtggcgaa gggacgccgt ttccggtaca ggctgatttc cgttcaggta atacccgtgt
    19561 tgcttttgat ggcgtcgtga atgacccaat gaagatgggc ggtgtcgatt tacggcttaa
    19621 attttctggc gattcactgg gtgatctcta tgaactgacg ggcgttctgc tgcccgatac
    19681 cccgccgttt gaaacggatg gtcggctggt agcgaaaatc gacactgaaa aatcgtcggt
    19741 ctttgattat cgcggtttta atgggcgaat tggcgatagc gatatccacg gttctctggt
    19801 ctacaccacc ggaaagccac gaccaaaact ggaaggtgat gtcgagtcgc ggcaattgcg
    19861 gctggcggac ctgggaccgt tgattggcgt tgattccggg aaaggggcag aaaagtcgaa
    19921 acggtctgaa cagaagaagg gcgaaaaaag cgttcagcct gcgggcaaag tgctgcctta
    19981 tgaccgcttc gaaaccgata aatgggacgt tatggatgcc gatgttcgct tcaaagggcg
    20041 gcgcattgag catggcagta gcctgccgat tagcgatctt tctactcata tcatcctcaa
    20101 aaatgctgac ctgcgcctgc aaccgctgaa atttggcatg gcgggcggca gcattgcggc
    20161 gaatattcat ctggaaggcg ataaaaagcc gatgcagggg cgggcagata ttcaggctcg
    20221 tcgactgaaa ctgaaagaac tgatgcccga tgtggaactg atgcagaaga cgctggggga
    20281 aatgaacggt gacgcggaac tacgcggtag cggtaactcg gtggcggcgc ttttaggcaa
    20341 cagtaacggc aacctgaaac tgttgatgaa tgacgggctg gtgagccgca acctgatgga
    20401 gattgttggg ctgaatgtcg gcaactacat tgtcggtgcg atatttggtg acgatgaggt
    20461 gcgggtgaac tgcgcggcgg cgaatctgaa tattgccaac ggcgtggcgc gcccgcagat
    20521 ttttgctttc gatactgaga acgcgttgat taatgttacc ggcacggcaa gttttgcttc
    20581 ggaacagctg gatttgacta ttgatccgga gagtaaaggg attcggatta tcacactgcg
    20641 ttcgccgctg tatgtgcgtg ggacgtttaa aaatccgcag gctggggtga aagccggacc
    20701 gctgattgcc cgtggtgctg ttgctgcggc actggcaacg ctggtaacac cggcggcggc
    20761 gttactggca ctgatctcac cttccgaagg ggaggctaat cagtgtcgga cgatattgtc
    20821 gcagatgaag aagtgatgat aattgccgga tgtctgacat ccggcaatta aacagattta
    20881 gatttttggc aaagtgtgct ttgttcatgc cggatgcggc gtgaacgcct tatccggcct
    20941 acaaatcctt gtgaattcaa tatattgtat ggaaattcag tcctgataag cgtagcgcat
    21001 caggcttttc acacttatta caacgactga tgtcgcgtct catgggtcag cagcagggcg
    21061 attaacgtca agccagccat cgccgccaga tataacccca ccgcacctaa cccgtagtta
    21121 gcctgcaacc aggctgcgat atacggcgca acggaagccc cgagaatcga cgatacgttg
    21181 taagagaacg atgctccggt gtaacgcact tctgtcggaa acagctctgg taacagcgca
    21241 cccatcggcc cgaaggtcag ccccatcaga cttaacccca gcagcaggaa ggcaaaaacc
    21301 agaatcgggt tgccagaacc gagcagcggg ttaaaggcga acagcgcgaa caggatgatc
    21361 agcgtggtga tgatcaccat gcttttacgg cgaccaaagg catcagccag taatccggcg
    21421 actggcacca tcacgccaaa accaataact gccatcatca gcatccacaa cacttcgtta
    21481 cgcggcaggc caagcccaac tggcgcggcg gcggtactga aggtcataga gtagaccgtc
    21541 atgatgtaaa acagcgtata ggttgccagc ataatgaacg tacccagtac ggttacgcga
    21601 acatgtttgg tcagcagcgt acccagcggg atcttcacct gttttttcgc tttagcgact
    21661 ttctcaaaca ccggcgactc atgcagcgac acgcgaacat acaggccgat aatgaccagc
    21721 accgccgaga agataaatgg cacacgccag ccccagctca taaactgctc atcggtcagt
    21781 agccaggaaa gcagcaagaa agtgccgttg gcaaagaaga agccaatcgg tgcgcctagc
    21841 tgtgggaagg agccatacag tgcacgtttg cgcggtgggg cgttttcagt cgccagcagc
    21901 gccgcgccgc cccattcacc gcctaagccc agaccctgac caaatcgagc cagcgccagc
    21961 agcagtgggg cgaaaatacc aatcgtggca tagcccggca gcagaccaat caccacggtc
    22021 gaaatcccca tcgttagcaa cgaggcgacc agcgtcgctt tacgcccaac gcgatcgcca
    22081 aaatgaccaa aaacggcaga gccaatgggg cgcgcgacga aggcgatggc gaaggtggcg
    22141 agcgactgta gcgttgctgc cgttggatcg ccctgcggga agaagatatg cggaaacaca
    22201 ataacggccg cagtggcgta aatgtaaaag tcgaagaact caatggccgt gccaatgaga
    22261 gaggcgacaa ggactttatt acgcgagttg atcggcgtgt attcttgttc gtggtcgagt
    22321 gttgtggctg ttgcttgcat aatcttttct tattttttgg ctaacgaata gccatattac
    22381 gcacagcaaa agtgatattt caatgtgtaa caaatcgcgg aaatggtcaa aaaccaggca
    22441 aaaaggggat aatgtttagg ccagtgattt ttcttctatg aaatgcttca cataatttta
    22501 taaatagtgg ataaaactgg tttcaggtta aataaaagtt accaactgga tttatatgct
    22561 ggggttatga gttggagtga aattgtttgc tcccaaatag atggctgaag aggctctggg
    22621 gaagagtttc aagaggttat gtgcgcatta tagccggatg cgatgctggc gcatcttatc
    22681 cggcctacgg ggagccaggc cgaataaaat cgtatgccgg ataaggcgtt tacgccgcat
    22741 ccggcctatt ttaaggctgc gttttccctg gcattcgcgg gtcgtcttta tattcggcgg
    22801 tggcaatcca cgccgcgcaa aacagcgtca aacgggcgaa gaagtagaaa aatgccatca
    22861 gccccagcac ggaaccaaat gctgcgccag agggggattt catcagcgat ggcagggtgt
    22921 aggtcatcac gattttaatc acttcaaaac caatagcagc cagaaatgtt ccgcgaatca
    22981 gggctttttt gcgcggacgg tggcgcggca accgccagaa gatccagaag aaaagcagat
    23041 agttggcgaa gatggaaatc gccaggccaa tcaatcgcca cgtcggtttc agccactcaa
    23101 tgctgttcag gtgcagggcg ctaataatca tttgctgcgc cgaaccggca accgaggtga
    23161 tcgaaagcgt cacaatcagc gcaatcaaca aaccaatcag tgaaataaaa tcacgcagat
    23221 attttaccca gaacttctcc tgatcttgcg gcgagcgttc ccagacatcg cgcgactggg
    23281 cacgaatcgc ttcacgcagg ttacccatcc agttgatgcc ggaataaagt gccaccgcca
    23341 ggccgacaag ccctacagtc gtacgctgct gaacggcggt gttgatggtg tttttcaacg
    23401 tggcggctag tgtcggatcg ctgatgtttt gcagaatttt gtcgaagata tcctgtagca
    23461 acatcggatg ggaggccagc acaaagcccc ctgcggcaaa cgacaccatc aaaatcggga
    23521 tcatcgacaa aaatgagaaa taggtgatag ccgcaccaaa ctggttaccc agccgatcgt
    23581 taaagcgttc tgtcgcgcga ataaggtgcg caataaccgg ttggcgttgg actttttcgg
    23641 cagtggtggt gacggtttcc agcgcctggc taactttgcc gcctttctcg ctattatcca
    23701 actgcttaat cggctcgtgc tccagatcct gggtgggacg tttgatctcg ttttcctgcg
    23761 tcatccgctc ttttgtcctt ctgtcgtggt cattcttaaa aagtatagtc ggtcagtcca
    23821 ggtgctcttt cattacgcca gccagccact gcataaacag gtttacacgc cgggaaagct
    23881 cccgacgctg cgggtaaacc agcgaaaggg agagcggctc ggcacggtag cctggcaaca
    23941 cttcaataag ccgcccggca cgcagggctt cgcgcacggc aatgcgcggg atctgaataa
    24001 tccccagacc ggcaagaccg gcggcgagat agttttcgct actgtttacc gtcaacatgc
    24061 cgccagactt aaaccactgg acgccattaa cgctggcaac ctcaaaacct aacggatgta
    24121 cacccaggtg cggtgtataa cgcactattg cgtgtgaagt cagatcatcg gggctttgcg
    24181 gataaccaaa gcgcgtcaga tagtgcggac tggcacagtt gaccatggtt agtttgccga
    24241 ggggacgggc gataacgcca tcttccggta acgcgccagt gcgtatcacg caatcaaaac
    24301 catcatgaag aatatctacc ggacggttat ggctactcag ttccagctca attcccggat
    24361 gctgatagag aaattccgac agacgcggca gtaacaggtt tttcgcgatt cctgacggga
    24421 tgtcgatgcg taatttaccg ctgatactgg tggcatcctg ttgaaacaga ccgtccagtt
    24481 cgctgagatt actcaacaca tcttttgccc gttgataata ggtcatgcct tctggcgtga
    24541 gtttgacccg tcgcgtagtg cgctggagaa gctgggtgcc aagttgatgt tccagtgcct
    24601 gtatctggcg cgaaacactt ccctttggca aagcaaagaa atccgccgcg cgggaaaaac
    24661 tttccagctc cgcgactttg atgaacaact gcattgcgtg aattttatcc atttctttga
    24721 ccgattgttg ttcacagcga aacagtaacg cgcatatcgc attgtttatt gttttttcta
    24781 acatctaatg ccgggtttca caatgttgtc atgacgaatg tggcgatttc tcagcaggaa
    24841 aagggcggaa tagctttatg agaaaggtga atccccttaa acagagcgta tttttcagtg
    24901 attcccttta aagtgaatag aggacgtatt ggctatttgt gcttattttt gtctcattgc
    24961 catttgttat tttggcttgt agtcgcggaa tttcattttt aattgcatgc gtactaacca
    25021 ccaatataag gaaatcctca aaattgtttt aaatagtgcc ctcgacatgt aatttcaggc
    25081 gtttagctgt aactatttat atcggtaact attttctacg cgtcatctct gtagaatggc
    25141 caccgccaaa tggcgaattc ttattttttc tcgcgggtaa tacccttcca ctacttaagt
    25201 ctttttaacg tattcaggtg gagattcagt tatgcaaata gtcatgtttg acaggcagtc
    25261 aatatttatt catggaataa aaatcagttt acagcagcgt attccaggag tgagtattca
    25321 gggggtcagt caggcagacg agttatggca aaagctggaa agttaccctg aagccttagt
    25381 tatgctcgat ggcgatcagg atggtgagtt ttgctactgg ttgctgcaaa aaaccgtggt
    25441 gcagtttcct gaggttaagg tgttaatcac ggcgacggat tgcaataaac ggtggttaca
    25501 ggaagttatc cattttaatg tgctggccat tgtgcctcgc gattcaaccg tcgagacatt
    25561 tgcgttggcg gtaaatagtg cggcgatggg gatgatgttt ctgcctggag actggcggac
    25621 gacgccggaa aaggacatta aagatctcaa atcgttaagc gcccgccagc gggagatttt
    25681 aaccatgtta gcggcgggcg aatcaaataa ggagatcggc agagcgctga atatcagtac
    25741 cggaacggta aaagcgcatc tggaatccct gtatcgccgt ctggaagtga aaaatcgcac
    25801 ccaggcggca atgatgttaa atatctcctc ctg
//
LOCUS       DADLTY010000044        25809 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.044, whole genome shotgun sequence.
ACCESSION   DADLTY010000044 DADLTY010000000
VERSION     DADLTY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.044"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..188)
                     /locus_tag="JN334_001533"
     CDS             complement(<1..188)
                     /locus_tag="JN334_001533"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7437714.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     SLSDGTVFEPVNSFQKNQ"
     gene            complement(453..626)
                     /locus_tag="JN334_001534"
     CDS             complement(453..626)
                     /locus_tag="JN334_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7437715.1"
                     /translation="MKRLQVFKFRLRPGGQQAREMRRFAGACRFVFSRTLARQNENHK
                     AGNKDIPYAKMAS"
     gene            694..1309
                     /gene="tnpA"
                     /locus_tag="JN334_001535"
                     /pseudo
     CDS             694..1309
                     /gene="tnpA"
                     /locus_tag="JN334_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1637..2719
                     /locus_tag="JN334_001536"
     CDS             1637..2719
                     /locus_tag="JN334_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HAY7437716.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3208..4006)
                     /locus_tag="JN334_001537"
                     /pseudo
     CDS             complement(3208..4006)
                     /locus_tag="JN334_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(4016..4567)
                     /gene="ybcL"
                     /locus_tag="JN334_001538"
     CDS             complement(4016..4567)
                     /gene="ybcL"
                     /locus_tag="JN334_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HAY7437717.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(4736..5068)
                     /gene="emrE"
                     /locus_tag="JN334_001539"
     CDS             complement(4736..5068)
                     /gene="emrE"
                     /locus_tag="JN334_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HAY7437718.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(5413..5727)
                     /gene="fliE"
                     /locus_tag="JN334_001540"
     CDS             complement(5413..5727)
                     /gene="fliE"
                     /locus_tag="JN334_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAY7437719.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            5941..7600
                     /gene="fliF"
                     /locus_tag="JN334_001541"
                     /pseudo
     CDS             5941..7600
                     /gene="fliF"
                     /locus_tag="JN334_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
     gene            7593..8588
                     /gene="fliG"
                     /locus_tag="JN334_001542"
     CDS             7593..8588
                     /gene="fliG"
                     /locus_tag="JN334_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAY7437720.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            8581..9267
                     /gene="fliH"
                     /locus_tag="JN334_001543"
     CDS             8581..9267
                     /gene="fliH"
                     /locus_tag="JN334_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAY7437721.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            9267..10640
                     /gene="fliI"
                     /locus_tag="JN334_001544"
     CDS             9267..10640
                     /gene="fliI"
                     /locus_tag="JN334_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HAY7437722.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            10662..11003
                     /gene="fliJ"
                     /locus_tag="JN334_001545"
                     /pseudo
     CDS             10662..11003
                     /gene="fliJ"
                     /locus_tag="JN334_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            11000..12127
                     /gene="fliK"
                     /locus_tag="JN334_001546"
     CDS             11000..12127
                     /gene="fliK"
                     /locus_tag="JN334_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HAY7437723.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            12232..12696
                     /gene="fliL"
                     /locus_tag="JN334_001547"
     CDS             12232..12696
                     /gene="fliL"
                     /locus_tag="JN334_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HAY7437724.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            12701..13705
                     /gene="fliM"
                     /locus_tag="JN334_001548"
     CDS             12701..13705
                     /gene="fliM"
                     /locus_tag="JN334_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAY7437725.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            13702..14115
                     /gene="fliN"
                     /locus_tag="JN334_001549"
     CDS             13702..14115
                     /gene="fliN"
                     /locus_tag="JN334_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAY7437726.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            14118..14483
                     /gene="fliO"
                     /locus_tag="JN334_001550"
     CDS             14118..14483
                     /gene="fliO"
                     /locus_tag="JN334_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HAY7437727.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            14483..15220
                     /gene="fliP"
                     /locus_tag="JN334_001551"
     CDS             14483..15220
                     /gene="fliP"
                     /locus_tag="JN334_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HAY7437728.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            15230..15499
                     /gene="fliQ"
                     /locus_tag="JN334_001552"
     CDS             15230..15499
                     /gene="fliQ"
                     /locus_tag="JN334_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HAY7437729.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            15508..16293
                     /gene="fliR"
                     /locus_tag="JN334_001553"
     CDS             15508..16293
                     /gene="fliR"
                     /locus_tag="JN334_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HAY7437730.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            16578..17201
                     /gene="rcsA"
                     /locus_tag="JN334_001554"
     CDS             16578..17201
                     /gene="rcsA"
                     /locus_tag="JN334_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HAY7437731.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(17245..17487)
                     /gene="dsrB"
                     /locus_tag="JN334_001555"
     CDS             complement(17245..17487)
                     /gene="dsrB"
                     /locus_tag="JN334_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HAY7437732.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDGGPRRPGVVLAVEE
                     FSEGTMYLVALEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            17596..17823
                     /gene="yodD"
                     /locus_tag="JN334_001556"
     CDS             17596..17823
                     /gene="yodD"
                     /locus_tag="JN334_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HAY7437733.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            18121..18936
                     /locus_tag="JN334_001557"
                     /pseudo
     CDS             18121..18936
                     /locus_tag="JN334_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(18933..20627)
                     /gene="yedQ"
                     /locus_tag="JN334_001558"
                     /pseudo
     CDS             complement(18933..20627)
                     /gene="yedQ"
                     /locus_tag="JN334_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(20798..20980)
                     /locus_tag="JN334_001559"
     CDS             complement(20798..20980)
                     /locus_tag="JN334_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HAY7437734.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(21059..21976)
                     /locus_tag="JN334_001560"
     CDS             complement(21059..21976)
                     /locus_tag="JN334_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAY7437735.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            22149..23069
                     /gene="yedA"
                     /locus_tag="JN334_001561"
     CDS             22149..23069
                     /gene="yedA"
                     /locus_tag="JN334_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HAY7437736.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(23058..23528)
                     /locus_tag="JN334_001562"
     CDS             complement(23058..23528)
                     /locus_tag="JN334_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HAY7437737.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(23509..24927)
                     /locus_tag="JN334_001563"
     CDS             complement(23509..24927)
                     /locus_tag="JN334_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAY7437738.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(24994..25534)
                     /locus_tag="JN334_001564"
                     /pseudo
     CDS             complement(24994..25534)
                     /locus_tag="JN334_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(25574..>25809)
                     /locus_tag="JN334_001565"
     CDS             complement(25574..>25809)
                     /locus_tag="JN334_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437739.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         6509 a         6614 c         6357 g         6329 t
ORIGIN      
        1 ttctggtttt tctgaaaact gtttacaggc tcaaagactg tgccatctga cagcgaggca
       61 agtttagcca cgccagcatc cagcccgacc attgatgctg aagggtgaac cggagttgat
      121 acttcacttt ctgcctgaat actgatgtac cacttaccgc aggactggct gacagtgaca
      181 tttttcacaa cacccgtgac ctgacggctg ttccggtagc gcatccagcc aagttttggc
      241 agaaaaatac ggctgttttc ctgagcgagc ttaacgccct gcggatagcg gaatgcatca
      301 ttctgtcacc gctttttgaa tcaggggaac gccgagcgct tctggaaaaa gtttttgcag
      361 gcctgctcag ggtctttcag tgactgttgc aatggctgtg acggagcatc tttaatccat
      421 tgcgtttcag tggcattttt ccactcaacc aatcaggaag ccattttcgc gtaagggatg
      481 tctttgttac cggccttatg attctcattc tgacgtgcca gagtacgact gaaaacgaaa
      541 cgacatgcgc ctgcgaagcg cctcatttca cgcgcctgtt gaccaccggg tcttaaccgg
      601 aatttgaata cctgtagtcg tttcacaggg caaattatac tttggtctgt gaaaaaaact
      661 gatatccgac gcggaaggca ctgtgttttc ctgatgcatg ctcaccgggt ctttgttacc
      721 aagtaccgac gccagatttt tgaccatgac gcgacagaaa aactacgcac ttacttttca
      781 aatgtatgtg cttattttga agctgaactg gttgaaatgg atggcgaacc agatcacgtc
      841 catctgttaa ttaactatcc ccccaaactg gcgatatcca gtctggtaaa caaacagcct
      901 caaaggcgta tcgggtaggt tactgcgacg ggatcgacca gatattgctg ccaggtatta
      961 ccgcaaagat gttttgtggc gtcccggcta ttttgccagt agctgcggag gtgcgccaat
     1021 atccgtcatc cgccaataca ttgaacaaca gcaaacacct ggtcagatga aaaaacgagc
     1081 cttatatccc cgccctgaag gacgggcttt tacggtgcac tggataagcc tggagattat
     1141 ctacacctgc atggacatca ttgtactatg ccccgtacat gctccatcac atcctcaaca
     1201 tcaaagtatt agagccttgc aaaccattat gcgtctcagt ttgtctcatc tatcttacgc
     1261 aaaaaaaata gaactaatat caagcggcgc tatattgcgt attaaataaa accacctcaa
     1321 ttacattaat aaatctaaat aaatatcatg caaataattt ccatggttat ttttaggcta
     1381 tttaaattaa aaaaataaat tatgagtcaa tcataaataa caacctttaa tataatttga
     1441 ctaccaaatt tatattcgtt cacaaaatag tcatgcattg tgtgcaatag aaacagttca
     1501 gataaaaata gggatctact tcacaattta aatgagaaac taaaacttac atcttgaaat
     1561 aatctcattg attggatgaa tatttgtagt gcagtgacat catttttaaa taatagttca
     1621 aataaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag ctgcatcagt
     1681 actgatggcg atgtctgctc aggcagcaga gatttacaat aaagacagta acaagctgga
     1741 tctgtacgga aaagttaatg ctaagcacta cttctcttct aacgatgcag atgatggcga
     1801 cacaacttat gttcgtctgg gcttcaaagg cgaaactcaa atcaacgatc aactgactgg
     1861 tttcggtcag tgggaatatg aattcaaagg caaccgtgct gaatctcaag gttcttccaa
     1921 agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct ccatcgatta
     1981 tggccgtaac tacggtgtag catacgatat cggtgcatgg accgacgttc tgccagaatt
     2041 cggtggcgat acctggaccc aaacagatgt gttcatgact ggtcgcacca caggtgttgc
     2101 aacctatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg ctgctcagta
     2161 tcagggtaaa aatgaccgca ctgacgtaac tgaagctaac ggtgatggtt tcggtttctc
     2221 cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat ctgaccgcac
     2281 taatgatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa atgctgaagt
     2341 atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca cctattctga
     2401 aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac aaaacttcga
     2461 agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt acctgcaatc
     2521 taaaggaaaa gacttgggtg cgtggggtga tcaggacctg attgaatata ttgatgtagg
     2581 tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa tcaacctgat
     2641 tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg ttgctgtagg
     2701 attggtttac cagttctaat ctgattactc aaagatatgt tgtgggaggc tttgcctccc
     2761 caacatataa gtggcgctct caagccactt tccttagaag cactatcttg cttctgacta
     2821 tataaattac cctttatttt ctgggacgtt tcaacgcccc atttttaata atactcagtg
     2881 aacaattaac gtatcaatta gatttaataa caacgatatc cgtacctaac ctgatatcta
     2941 atgtcataag catcccttta attatcccct caacctcttg taaatttccc actcttgagc
     3001 tccacatagc ccaaacgccg gatgcaaaaa aaatcatacg tgcatctctg aaattgtgat
     3061 ggaggaagtg agtatcaatt atatgtgctt ataaacgttc ccgattggca cagaagtcac
     3121 cggattgttc aggctccaat aggttgagta tgtcgtattt attttccaaa ataaaaatcg
     3181 atgctgttgc tccttaataa aatactttta ctctctggac gcttttctta gctctcttgg
     3241 tgttatatta aaacgattat ggaacctttc agtaaaacga gaaggacact tgtaaccatt
     3301 ttccctggca atctcgctga tgggttttac tgtcgtttgt atagcaggac aacgcattat
     3361 ttaacctcac gtcgtccaat atactttgaa aacttattcc ctcgcttgcc agacggcgat
     3421 gcaatgtgga aacagaaatg tagagatatc tcgctacctt gtttgctgtc cattttgttc
     3481 taggttcgga tagcattagg ttataacaac gacttattaa tgattgttta ctatctgata
     3541 aaagtaaaca attaacatga tgtgctccta atgaaagtag aacgcccatt gctaagtgtt
     3601 cctgaatttg cgttgagaag cctcgggaaa tagatgtttt tagttgctcc caacaatata
     3661 ttaactcggg gttctgaggt aagcagaaac ctgtcgtgtt acgaatttga tcagttatcg
     3721 cataaaattt ttgaaacttt tcaattagat caatgggata ataaagcatt tctgcaagat
     3781 aaagacctgc ttctggataa ttcacaatat aaaattcata tccacaggga aataatatta
     3841 tctgattatt atcaacagtt agactatgcg tctcccaatt gataactttt tttccctgac
     3901 ggatacgaca taaagctggc ataagaggct taaccctgtg aatctcatga tgtttatgca
     3961 tccgtatttc ttcaatcttt aggttagact tatctcttgc cagcatactc ttaccctatt
     4021 ttatctcata aactggtgtt atctcagcgg ttgcaatttt attggcatta agcatataac
     4081 caactaacgc tccgctggaa ttagaatcca caggaatctt atcagttttt agagcccata
     4141 ctttaaactg gtaatgatgg ggtttatctc ctttgggagg acatgctcca ccaaatcctg
     4201 cataaccaaa atcatttcgg ccttgaacag caccagtcgg aagttttgta ccatcacgtc
     4261 ttcctgcatc agtgggcaaa tatgttacag ttgctggaat attagcaaca gtccagtgcc
     4321 accatccact gcctgtagga gcatctggat catacacagt tacggcaaag cttttggtgc
     4381 cttcaggagc accagaccag gttaatgagg gcgatgtatt accaccttca cacccaaatc
     4441 cagaaaatac atgagacgtt gtaagttgct ctcctgtttt tatttcatta ctagtgacct
     4501 gaaatgctgc ggcctgcgca gaaaatgtta tgaatgccaa tacacttgaa acgataagtt
     4561 ttttcataaa aacctcattg ttgtgaccta tcgttatttt atttgatgtg ccttgatctc
     4621 attatgcata aaggcgcaat gatcgcgcaa atgcaatcat aattataaag gtgccccgaa
     4681 cattagccat aatatgcaca aatcagattc ctgttatctc aaggagaatt attttttaat
     4741 gcggtgcact tcgtgacaac aagttaatga ctaacacacc ggcacaaatc aacatcatgc
     4801 ctatgatagc tggcaggtcc agtcgttgac caaaaaatcc ccaggaaagc aaactaatca
     4861 gaacgatacc gactcctgac cagatagcat aagcaatccc tgtaggtata taagccagcg
     4921 tctgagctaa taaccagaat gatgcacaat agcaaataat tgtgccaaca gagggccata
     4981 accgtgtaaa cccctctgaa aacttcatta aggttgtacc aatgacctct gcaagtattg
     5041 cgccaccaag ataaatataa gggttcatag catattcttt cctgttcata ctggagagaa
     5101 ttgtactaca gtttgaacac aactcaccgt tttcatcctg gtgtatgtgt atccaagggg
     5161 ctctggcgtg gcaagagata aaggcaggaa caataatgat cagaaggaac cccaggctaa
     5221 cgaatgagtt cactcttcct gggagcaaag cgaagacaaa acatgttatc gcgtttacgg
     5281 atcgtggatg gcagaaaaca accaggatca ggtatccatt ttaaaccaga aattgagtga
     5341 gtttgcccca tccatgcccc acgctgtggg atcggatata attaaacaag catagtcaac
     5401 aggttacaca acctacacct gcatggacat cacttcctga tacgccgcca ccagcttatt
     5461 acgcacctga atccccattt gcatagaaac tgaggctttt tgcatatcgg tcatcacatc
     5521 gtttagcgcc acgccgggtt caccgagggt gaatttttct gcctgcgtgc gggcgactgt
     5581 ttgtgtatcg cttatgcgat cgagcgcggc gtgcagctgc ccggcaaaac taatggtcgg
     5641 ttgcggcagt gattcctgcg cacgcgcact catcgccgta gcctgtaact ggctgataac
     5701 cccttcaatc ccctgtatcg ctgacatttt cgtctcccgg ataatttctg gtaacaaagc
     5761 ctaccagtaa gtcaataaga caaaggcgct aaatagcaac aaaaaaactg gtttattggc
     5821 ggatagaaaa aacgaaagca caaataatgg gagcgtcaat ttttcgagtg tgctgacccg
     5881 agagtgagtc ttgttccact ttgccaataa cgccgtccat aatcagccac gaagtgcgcg
     5941 atgaatgcga ctgcagccca gacaaaatct cttgagtggc ttaatcgcct gcgtgcgaat
     6001 ccgaaaattc cattgattgt taccggttcc gcggcagtgg cggtcatggt cgcactgatc
     6061 ctgtgggcga aagcccccga ctaccgcaca ttattcagca atctttccga tcaggatggt
     6121 ggcgcaattg tcagccaact gacgcaaatg aatattcctt accgcttcag cgaagccagc
     6181 ggcgctattg aggttccggc agataaagtt cacgaactgc gcctggcgca acaaggtttg
     6241 cccaaaggcg gcgcggtcgg tttcgaactg ctcgatcagg aaaagtttgg tatcagccag
     6301 ttcagcgaac aggtgaatta tcagcgggcg ctggaaggcg agctttctcg taccatcgaa
     6361 actatcggcc cggtaaaagg ggcgcgcgta catctggcaa tgccgaaacc gtctttattc
     6421 gtccgtgaac aaaaatcccc ttctgcatcg gtgacggtaa atctgttacc cggccgcgca
     6481 ctcgatgaag ggcaaattag cgctattgta catctggttt ccagcgcggt tgctggtctg
     6541 ccgcctggaa acgtcacgct ggtggatcag ggcgggcatc tgttaaccca gtccaatacc
     6601 agcgggcgcg atcttaatga cgctcagttg aaatatgcca gcgatgtcga aggccgtatt
     6661 cagcagcgta ttgaagcgat cctgtcacct attgttggta acggtaatat tcacgcctag
     6721 gtcacggcgc aactggactt cgccagtaaa gaacaaacgg aagaacagta tcgccctaac
     6781 ggtgatgaat ctcatgcggc gcttcgttca cgccagctta atgagagcga gcaaagcggt
     6841 tccggttatc cgggcggcgt accgggggcg ttgtcgaatc aaccggcacc tgcgaataac
     6901 gcgccaatca gcacgcctcc gacaaatcaa aataaccgcc agcagcaggc gagcaccacc
     6961 agcaatagtg ggccgcgtag cacacagcgg aatgaaacca gtaactacga agtcgatcgc
     7021 accattcgtc ataccaaaat gaacgtgggc gatgtgcaac gtctgtcagt cgcggtagtg
     7081 gtgaattaca aaaccttgcc agacggtaaa ccattgcctc tcagcaacga acagatgaag
     7141 caaattgaag ctctgacccg cgaggcgatg ggcttttctg aaaaacgcgg tgattcgctc
     7201 aatgttgtta actcgccgtt caatagcagt gacgaaagcg gcggagcact gccgttttgg
     7261 caacagcaag tgtttatcga tcagttgctg gctgccggtc gctggttgct ggttgctggt
     7321 actgctggtg gcgtggttgc tgtggcggaa agcggtacgc ccgcagctaa cacgtcgcgc
     7381 cgaggcggtg aagactgtgc agcaacaggc gcaggcccgc gaggaagtgg aagatgcggt
     7441 ggaagtccgt ctgagcaaag acgaacaact ccaacaacgg cgcgctaacc aacgactggg
     7501 ggcagaagtc atgagccagc gtatccgtga aatgtctgat aacgatccgc gcgtggtggc
     7561 gctggtcatt cgccagtgga taaataacga tcatgagtaa cctgacaggc accgataaaa
     7621 gcgtcatcct gctgatgacc attggcgaag accgggcggc agaggtgttc aagcacctct
     7681 cccagcgcga agtgcaaacc ctgagcgctg caatggcgaa cgtcacgcag atctccaaca
     7741 agcagctaac cgatgtgctg gcggagtttg agcaagaagc tgaacagttt gctgcactga
     7801 atatcaacgc caacgattat ctgcgttcgg tattggtcaa agctctgggt gaagaacgtg
     7861 ccgccagcct gctggaagat attctcgaaa cccgcgatac cgccagcggt attgaaacgc
     7921 tcaactttat ggagccgcag agcgccgccg atctgattcg cgatgagcat ccgcaaatta
     7981 tcgccaccat tctggtgcat ctgaggcgcg cccaggccgc cgatattctg gcgttgttcg
     8041 atgaacgtct gcgccacgac gtgatgttgc gtatcgccac ctttggcggc gtgcagccag
     8101 ccgcgctggc cgaactgacc gaagtactga atggcttgct cgacggtcag aatctcaagc
     8161 gcagcaaaat gggcggcgtg agaacggcag ccgaaattat caacctgatg aaaacgcagc
     8221 aggaagaagc cgttattacc gccgtgcgtg aattcgacgg cgagctggcg cagaaaatca
     8281 tcgacgagat gttcctgttc gagaatctgg tggatgtcga tgatcgcagc attcagcgtc
     8341 tgttgcagga agtggattcc gaatcgctgc tgatcgcgct gaaaggggcc gagcagccac
     8401 tgcgcgagaa attcctgcgc aatatgtcgc agcgtgccgc cgatattctg cgcgacgatc
     8461 tcgccaagcg tggtccggtg cgtctgtcgc aggtcgaaaa cgaacagaaa gcgattctgc
     8521 tgattgtgcg ccgtcttgcc gaaactggcg agatggtgat tggcagcggc gaggatacct
     8581 atgtctgata atctgccgtg gaaaacctgg acgccggacg atctcgcgcc accaccggca
     8641 gagtttgtgc caatggtcga gtcggaagaa accattattg aagaggccga acccagcctt
     8701 gagcagcaac tggcgcaact gcaaatgcag gcccacgagc agggttatca ggcggggatt
     8761 gccgaaggtc gccagcaagg tcacgaacag ggctatcagg aaggactggc ccaggggctg
     8821 gaacaaggtc tggcagaggc gaaggctcaa caagcgccaa ttcatgcccg gatgcagcaa
     8881 ctggtcagcg aatttcaaac tacccttgat gcgcttgata gtgtgatagc ttcgcgcctg
     8941 atgcagatgg cgctggaggc agcacgtcag gtgatcggtc agacaccaac ggtggataac
     9001 tcggcactga tcaaacagat ccaacagttg ttgcagcaag aaccgttatt cagcgggaaa
     9061 ccacagctgc gcgtacaccc ggatgatctg caacgtgtgg atgatatgct cggcgcaact
     9121 ttaagtttgc atggctggcg cttgcggggc gatcccaccc tccaccctgg cggatgtaaa
     9181 gtctccgccg atgaaggcga tctcgacgcc agtgtcgcca ctcgctggca agaactctgc
     9241 cgtctggcag caccaggagt ggtgtaatga ccacgcgcct gactcgctgg ctaaccacgc
     9301 tggataactt tgaagccaaa atggcgcagt tgcctgcggt acgtcgctac gggcgattaa
     9361 cccgcgctac cgggctggtg ctggaagcca ccggattaca attgccgctc ggcgcaacct
     9421 gtgtcattga gcgccagaac ggcagcgaaa cgcacgaagt agaaagcgaa gtcgttggct
     9481 ttaacggtca acggctgttt ttaatgccgc tggaggaagt ggaaggtgtc ctgcccggcg
     9541 cgcgtgtata tgccaaaaac atttcggcag aagggctgca aagcggcaag cagttgccgc
     9601 tcggtccggc gttattaggt cgtgttctgg acggtagcgg taaaccgctc gatggcctgc
     9661 ccttccccga tacgacggaa accggtgcgc tgattacccc gccatttaac ccgttgcaac
     9721 gtacaccgat tgaacatgtg ctggacaccg gcgtgcgccc aatcaatgcc ctgcttaccg
     9781 tcggacgcgg gcagcgtatg gggctgtttg ccgggtcggg cgttggtaaa agtgtgctgc
     9841 tggggatgat ggcacgctac acccgcgccg atgtcattgt cgtgggtttg attggtgaac
     9901 gtgggcgcga agtaaaagat tttattgaga atatcctcgg tgccgaagga cgtgcacgct
     9961 cagtggtgat tgccgctccg gcggatgttt ctccgctcct gcgaatgcaa ggtgccgcct
    10021 atgccacgcg cattgccgaa gattttcgcg atcgtggtca gcatgtgttg ctgattatgg
    10081 actccctcac ccgctacgcg atggcccagc gtgaaattgc gctggcgatt ggcgaaccac
    10141 ctgccactaa aggttatcca ccgtcggtgt ttgccaaatt accggcactg gtcgagcgtg
    10201 ccggtaatgg cattagcggc ggcggctcga ttaccgcgtt ttataccgtg ctcaccgaag
    10261 gcgatgacca gcaggacccc attgctgact ccgcgcgggc aatcctcgac ggtcacattg
    10321 tgctgtctcg ccgactggcg gaagccgggc actatccggc tatcgatatt gaagcgtcga
    10381 tcagtcgcgc aatgacggcg ttgatcagtg agcaacatta cgcgcgagtg cgcaccttca
    10441 aacagctgtt gtcgagtttt cagcgtaacc gcgatctggt tagcgtcggc gcgtatgcca
    10501 aaggcagcga tccgatgctc gataaagcca tcgccctgtg gccgcagctg gagggctatt
    10561 tgcaacaagg catttttgaa cgcgcggact gggaagcgtc tctccagggg ctggagcgta
    10621 ttttcccgac agtgtcataa cccaggagat aacggcagat ggcggaagaa cagctcaaaa
    10681 tgttgatcga ttatcagaat gaatatcgca ataacctcaa tagcgatatg agtgccggga
    10741 tgaccagcaa ccgctggatc aactatcagc agtttatcca gacgctggaa aaagccatta
    10801 ctcagcatcg ccagcaactt aatcagtgga cgcagaaagt tgacattgcc ctgaacagct
    10861 ggcgagaaaa gaaacaacgt ttgcaggcct ggcagacact gcaggaacgg caatccacgg
    10921 cggcactgct tgcagaaaac cgcctcgatc agaaaaagat ggatgagttc gcccagcgcg
    10981 ccgccatgag gaaacctgaa tgattcgctt agcgcccttg attaccgccg acgttgacac
    11041 caccacattg cctggcggca aagccagcga tgctgcacaa gattttctca cgttgttgag
    11101 cgaagcatta gcaggcgaga caacaactga caaagcggcc ccccagttgc tggtagcaac
    11161 agataagccc acgacaaaag gcgagccgct ggtcagcgat attcttgccg atgcgcaaca
    11221 agcggattta ctgatccctg tggatgaaac acttcctgtc atcaacgacg aacaatccac
    11281 atcaacaccg ttaaccaccg ctcagacgat gacgttggct gcggtggctg acaaaaatac
    11341 gacaaaagac gaaaaagcgg atgatcttaa tgaagacgtc accgcaagtc tgagcgccct
    11401 ttttgcgatg ttgccgggtt ttgacaatac gcccaaagtg actgatgcac cgtcaaccgt
    11461 gttaccggca gagaaaccaa cgctcttcac aaaactgact tctgcgcaac tcactacagc
    11521 acagcctgat gacgcccccg gcacaccagc tcagccatta acaccgctgg tagcagaagc
    11581 ccagagtaaa gcggaagtca tcagcacgcc ttcgccggtg accgctgacg ccagcccgct
    11641 aatcactcca caccagacac agccactgcc caccgtcgcc gcgcctgttt tgagtgcacc
    11701 gctgggttct cacgaatggc aacaatcatt gagccagcat atttcgctgt tcacccgcca
    11761 ggggcaacaa agtgcagagt tgcgtctgca cccacaggat ttaggtgaag tgcaaatttc
    11821 cctcaaagtg gatgataacc aggcgcagat ccagatgatt tcaccgcatc agcacgtacg
    11881 cgccgccctg gaagcagcgc tgccggtact gcgtacgcag ctggccgaaa gtggcattca
    11941 gttagggcaa agcaacatta gtggcgaaag ctttagtggt cagcagcagg ccgcttccca
    12001 gcaacagcaa agccaacgca cagcaaacca tgaacctctg gcgggggaag acgacgatac
    12061 gcttccggtt cccgtctctt tacaagggcg tgtaacaggt aacagcggcg ttgatatttt
    12121 cgcctaacgt cagaggtagc accgtaatcc gcgtcttttc cccgctttgt tgcgctcaag
    12181 acgcaggata attagccgat aagcagtagc gacacaggaa gaccgcaaca catgactgat
    12241 tacgcgataa gcaagaaaag caagcgatcg ctttggatcc cgattctggt attcattacc
    12301 ctcgcggcct gtgccagcgc aggttacagc tactggcatt cgcatcaggt tgccgctgac
    12361 gacaaagcgc agcaacgcgt cgtgccctcc ccggtcttct atgcgctgga tacctttacg
    12421 gtcaatttgg gcgatgcgga tcgcgtgctt tatatcggca taaccctgcg actgaaagat
    12481 gaagcaaccc gctcgcggct gagtgagtat ttgccggaag tccgtagtcg cttgctgtta
    12541 ctgttttcac gtcaggatgc tgccgtactg gcgacagaag aaggcaagaa aaacctgatt
    12601 gccgagatta aaaccacact ttccaccccg cttgttgccg ggcaaccgaa acaggatgtc
    12661 accgacgtgc tgtataccgc ttttattctg cgataacgac atgggcgata gtattctttc
    12721 tcaagctgaa attgatgcgc tgttgaatgg tgacagcgaa gtcaaagacg aaccgacagc
    12781 cagtattagc ggcgaaagtg acattcgtcc gtacgatccg aatacccaac gacgggttgt
    12841 gcgcgaacgt ttgcaggcgc tggaaatcat taatgagcgc tttgcccgcc attttcgtat
    12901 ggggctgttc aacctgctgc gccgtagccc ggacattacc gtcggggcca tccgcattca
    12961 gccgtaccat gaatttgccc gcaacctgcc ggtgccgacc aacctgaacc taatccacct
    13021 gaaaccgcta cgcggcacag ggctggtagt gttctcgccg agtctggtgt ttatcgccgt
    13081 ggataacctg tttggcggcg atggacgctt cccgaccaaa gtggaaggtc gcgagtttac
    13141 ccatactgaa cagcgcgtca tcaaccgtat gttgaagctg gcgctggaag gctatagcga
    13201 cgcctggaaa gcgattaatc cgctggaagt tgagtacgtg cgttcggaaa tgcaggtgga
    13261 atttaccaat atcaccacct cgccgaacga cattgtggtt aacacgccgt tccatgtgga
    13321 gattggcaac ctgaccggcg aatttaatat ctgcctgcca ttcagcatga tcgagccgct
    13381 acgggaattg ttggttaacc cgccgctgga aaactcgcgt aatgaagatc agaactggcg
    13441 cgataacctg gtgcgccagg tgcagcattc acagctggaa ctggtggcca actttgccga
    13501 tatctcgcta cgcctgtcgc agattttaaa actgaaaccc ggcgacgtcc tgccgataga
    13561 aaaacccgat cgcatcatcg cccatgttga cggcgtcccg gttctgacca gtcagtacgg
    13621 tactctcaac ggtcagtatg cgttacggat agaacatttg attaacccga ttttaaattc
    13681 tctgaacgag gaacagccca aatgagtgac atgaataatc cggccgatga caacaacggc
    13741 gcaatggacg atctgtgggc tgaagcgttg agcgagcaaa aatcaaccag cgaaaaaagc
    13801 gctgccgacg cggtgttcca gcaatttggc ggtggtgatg tcagcggaac gttgcaggat
    13861 atcgacctga ttatggatat tccggtcaag ctgaccgtcg agctgggccg tacgcggatg
    13921 accatcaaag agctgttgcg tctgacgcaa gggtcagtcg tggcgctgga cggtctggcg
    13981 ggcgaaccgc tggatattct gatcaacggt tatttaatcg cccagggcga agtggtggtt
    14041 gttgccgata aatatggcgt gcggatcacc gatatcatta ccccgtccga gcggatgcgc
    14101 cgcctgagcc gttagtgatg aataaccatg ctactgtgca atcttccgcg ccggtttctg
    14161 ctgagccact gctgcaggtg agcggcgcac tcatcgccat tattgccctg atcctcgctg
    14221 ctgcctggct ggtaaaacgg ctgggatttg cccctaaacg cactggcgtt aacggtctga
    14281 aaattagcgc cagtgcttcg ctgggcgcgc gtgaacgggt cgtggtggtc gatgtggaag
    14341 atgcacggct ggtgctcggc gttaccgcag ggcaaatcaa tctgctgcat aaacttcccc
    14401 cttcagcacc aacggaagag ataccgcaga ccgattttca gtcggtcatg aaaaatttgc
    14461 ttaagcgtgg cgggagatcc tgatgcgtcg tttgttttct gtcgcaccgg tccttctctg
    14521 gctgattacg cccctcgcct tcgcgcaact gccgggtatc accagccagc cgctacctgg
    14581 cggtggacaa agctggtcgc tcccggtgca gacgctggtg ttcatcacct cgttgacgtt
    14641 cattccagcg attttactga tgatgaccag tttcacccgc atcattattg tttttggttt
    14701 attgcgtaac gcgctgggaa cgccctccgc gccacctaac caggtattgc tggggctggc
    14761 actgtttttg acctttttta ttatgtcgcc agtgatcgac aaaatttatg tcgatgcgta
    14821 ccagccattc agcgaagaga aaatctcaat gcaggaggcg ctggaaaaag gggcgcagcc
    14881 gctgcgtgag tttatgctgc gtcagacccg cgaggcagat ttagggttgt ttgccagact
    14941 ggcgaatacc ggcccgttgc aggggcctga agctgtgccg atgcgcattt tgctcccggc
    15001 ctacgtgacc agcgagttga aaaccgcatt tcagataggc ttcacgattt tcatcccttt
    15061 tttgattatc gacctggtga ttgccagcgt gttgatggca ttggggatga tgatggttcc
    15121 ccccgccacc attgctctgc cctttaaact gatgctgttt gtactggtgg atggctggca
    15181 attgctggtc ggttcgctgg cgcagagctt ttacagctag agaggcaaaa tgacacctga
    15241 atcggtcatg atgatgggaa ctgaagcgat gaaagtcgcg ctggcactgg cagccccgct
    15301 gttgctggta gcgctggtca cgggccttat catcagtatt ttgcaggcag ccacgcagat
    15361 taacgaaatg acgctgtcgt ttattccgaa aatcatcgcc gtatttatcg ccatcattat
    15421 tgccggaccg tggatgctca atctgttgct ggattacgtc cgcaccttgt tcactaacct
    15481 gccgtatatc atcgggtagc ccgtacaatg atgcaggaga caagcgatca atggctttcc
    15541 tggttaagcc tgtacttctg gccgttactg cgcgtgctgg cgctcatttc taccgcgcca
    15601 attctgagcg aacgcagcgt gccgaaacgg gtaaaactgg gtctggcaat gatgatcacg
    15661 ttcgccattg ccccatcatt acctgccaac gatgttcctg ttttttcgtt ctttgctctg
    15721 tggctggccg tacagcagat cctgatcggc attgcgcttg gttttaccat gcaatttgcc
    15781 tttgccgctg tgcgaaccgc tggcgaaatt atcggtctgc aaatggggct gtcatttgcg
    15841 acatttgtcg atcctggcag ccatctcaat atgcccgttt tagcgcgcat catggatatg
    15901 ctggcattac tgctgttcct gacatttaac ggtcatttat ggttgatttc actgctggtc
    15961 gatacctttc acaccctgcc gattggtagc gaaccgttga acagcaatgc gtttctggca
    16021 cccaccaaag cagggagttt gattttcctt aacgggctga tgctggcgtt accgctcatt
    16081 actctgttgc tgaccctgaa tctggcatta ggtttactga atcgtatggc cccgcaatta
    16141 tccatttttg ttattggatt tccattaact ctgactgtcg gcatctcttt aatggcggca
    16201 ttaatgccgt taattgcgcc tttttgcgaa catttattca gtgaaatttt taatttgctg
    16261 gctgatatta ttagtgaatt accattaata taattccgta acgtttatca tgctacccta
    16321 aggattatcc gaaaaataat atctgcgaac atcttccagg atactcctgt agcgaaatat
    16381 ttgttttaag ctcactcaca tatcgcaaca tttactttaa gacaattcca ggcaaattat
    16441 acaacacttt acgggatagt aagtccgcct gaaaaattgc gagagtggcg cattaggtga
    16501 cccatgttgt tccgtttagt catgatgaaa tattcaggta aggggaatta tcgttacgca
    16561 ttgagtgagg gtatgccatg tcaacgatta ttatggattt atgtagttac acccgactag
    16621 gtttaaccgg gtatctgttg agtagagggg ttaaaaaaag agaaatcaac gacattgaaa
    16681 ccgttgatga ccttgccata gcttgtgatt cacagcgtcc ttcagtggtg tttattaatg
    16741 aggactgttt catccacgat gcttctaaca gtcagcgcat caagcacatc attaatcaac
    16801 atcccaatac gttatttatc gtttttatgg caattgccaa tgttcatttt gatgaatatc
    16861 tattggtcag aaaaaattta ttgatcagtt ctaaatcgat taaaccggaa tctctcgacg
    16921 atatccttgg cgatattctg aaaaaagaga caacgataac ctcgttttta aatatgccga
    16981 cgttatcatt gagccgaacc gaatcgagta tgttgcgaat gtggatggca ggtcagggaa
    17041 ccattcaaat ctccgaccaa atgaatatca aagccaagac cgtttcatcg cataaaggta
    17101 atattaaacg taagatcaaa acgcataata aacaggttat ctaccatgtc gtccgactga
    17161 cggataatgt gactaatggt atttttgtca acatgcgcta acacattctg actggtggtt
    17221 tcccaccagt caggctgaat aagattactc tgctttctca acaaagatac cgtcctgatg
    17281 ccctgcttca ttaaagaacc agatgccgag cgggtagtct tccagcgcaa ccaggtacat
    17341 tgtgccttca ctaaactcct caactgccag taccactcca ggacgacgcg gaccgccatc
    17401 cgttttgact gttacccgat cattcacctt catcgttttc ctcctgtggc tttgtgccag
    17461 tgtagaacaa tttcgttttt ctggcagcgc caagcgcgcg cgagtgctga ttttctcgac
    17521 ggtctatact taagagatgc caggcggact taacgactgg cggcaacaac agagtaacgg
    17581 ttgcgaggaa agatgatgaa aaccgcaaaa gtgtacagcg ataccgcaaa acgtgaggtc
    17641 agcgtcgatg tcgatgccct gctggcggcg atcaatgaaa ttagcgaaag cgaagttcat
    17701 cgcagccaga acgattctga acacgttagc gtcgatggac gtgaatatca tacatggcgt
    17761 gaattggcgg atgccttcga actggatatt catgacttca gcgtctctga agtgaatcgt
    17821 tgaatgcaca ataaaaaaat cccgaccctg agggtgtcgg gatgaaactt gcttaagcaa
    17881 gaagcactta aaaaattcgt tacaccagga aatctgatgt gttcatcacc ttatctgcaa
    17941 tttttttcgc tgacaagaaa atattcgcgc tatgaatgat taacgctact tatgaataga
    18001 aatgtgacgc gcgtcacccc ttggtggctc tggtgtcaac gggatcgctc ccgaaaaaat
    18061 catctttgat tatttgtgct gttttgagaa tcatcctggt aaggggagta ctgcgcgacc
    18121 atgttttcaa ttcaacaacc actactggtt tttagcgatc ttgatggcac cctgctggac
    18181 agtcatagtt atgactggca accggcagcc ccctggctca gccgtttaca cgaagccaat
    18241 attcccgtca ttctctgtag cagtaaaact ttagcggaaa tgctgtactt gcaaaaaatg
    18301 ttggggctac aaggcttacc gctgattgca gagaatggcg cagtgatcca gcttgctgaa
    18361 caatggcagg atatagacgg ttttccacgc atcatctcag gtattagcca tggcgaaatc
    18421 tgccaggttt taaatacgct acgcgagaaa gaacatttta aattcacgac ttttgatgat
    18481 gtcgacgatg caaccattgc cgaatggacg ggattaagcc gtagctaggc ggcgctgacg
    18541 cagctgcatg aggcgtcggt aacgctaatc tggcgcgaca gtgacgagcg tatggcacaa
    18601 tttatcgctc gtctgaacga actgggcttg cagtttatgc agggtgcgcg cttctggcac
    18661 gtactggatg cctctgccgg aaaagatcag gctgccaact ggattatcgc gacctatcaa
    18721 caattgtcag gcagacgccc aaccacactt ggcctgggcg atgggccaaa cgatgcgcct
    18781 ttactggagg taatggatta cgcggtgatt gtgaaagggc taaaccgtga aggggtacat
    18841 ctgcatgatg aggatccggc ccgcgtctgg cgaacgcagc gtgaaggacc ggaaggctgg
    18901 cgtgaagggc tggaccattt tttctccgcc cgttaagcgt tatcgctcgc gaatacccga
    18961 ttgcgcccgg cctgttttgc gaggtaaagc cgacggtcgg ccaatgactg gagttgttca
    19021 aaatcataat caccggtttc ctcgctgcta cttaccccca gcgaggcact gatgcgtatc
    19081 gttgtactct tggcgatcag catctctttt tcatttaact tcaggcgaat acgttctgcg
    19141 acctccgcag cctgcgtcag attcgcgcct ggcagaatca cacaaaactc ctcaccaccg
    19201 acccgcccgg caacgtcctg cgcacgcaag gaactgctaa ttaatccggc agcatgagaa
    19261 agaacacggt cgcccgcctg atgaccaaag cggtcattaa tcgctttaaa atggtcaaga
    19321 tcgacctgaa tgacagaaaa aggatgttgg tgcgtctgac acaatttagc gagcggacgg
    19381 gctttttcga acagtgcgcc acggttatat aaacgcgtta aggtgtcgtg ccacgcctgc
    19441 cactgcaacg agctttgcag aacatacatg ttgctgacca tccggcgaat cacataccag
    19501 gagaggagta acatggtggt aaagagcgcc cacagcaggg ttaatgcaat gctgatactg
    19561 ccgaaatcgc cgcgcacgcc ttcgcttagc gtatggacgc gcgccagcac accgtcgaaa
    19621 tggtccagac gttcccagct aacatagcga ctgtccatac gaatgccgcc ccgcgtgtca
    19681 tgttccattg cctgcgccag caaggccagt tcacgaggat caaaaatatt ccctgttgga
    19741 tggtcaggat tggaagaggt caaaaatctc agcttactgt catagagctg atactcacca
    19801 tcgaggtttt tatcgatggc gtttcttaaa aattgctgca tagtacgcac gggaatactc
    19861 atccccagca cgccatacca gtagttatta ctgtctaccg gaacactgac ggtaacctgc
    19921 ggttcagtat tgctggcgtg ttccggttgc gaagtgaacc agcgtaccgc gcggtgacga
    19981 ttttctcgtt gcgaatggcc gataaaccag ggttgggtga catagccgta ataacgcgtt
    20041 ggtacattgc gcgtaaacaa ggtcggctgc gtcgaaacgt aaaatccagc acgcgagaca
    20101 tacatcgcct gttcaaccat cgacgaggag ttgtgcgcca gtcgcagcaa gtaaccaact
    20161 tccagtgcag cggtaatttc attgtcgagg ctttcatttt cgcgagacag gaaattcccc
    20221 tcgctgacta atgcatccga tacgccattg actggcaggg tgcgccgtcg gttgagttcg
    20281 atttgccagg cgtgctcgtc gcgatgctgt tcgaactcgg ttacggcatt acgcagagag
    20341 gtgaaatcca gtggcgccac gagagcttcg cgcatgccat tacgcaaaaa gatcagtttg
    20401 tcgacgttat actgcaaatg cttatcgagc gcgttggcta cgttttccag atgattacgc
    20461 tagctggaga tataggcatc ttccagcacc accacttcac gccaggtgag caaggtggaa
    20521 aaaagcaata ccacgataaa gcagagatta acgacatgac cgggccccag gcggcgtgcg
    20581 agttttttca accagctctg gttttccatt tttgtctcgt gctgcaccct gacgactccc
    20641 aacctgcttt tttatgattc tggcataacc cggcaatagc gtaccgcaaa acagcgataa
    20701 ttttataatg gtggcatgat aagacgcgac tatcattcat tgcaggcatt gggacaccag
    20761 gccggatgtg atccggcctg gtgttaatga taccctttca aacttcttcc gcagaacgac
    20821 tcccctcccc cggaacaaga tcggtttcat gaaaagcttc ccgcttgacc ccgtaaccgt
    20881 cataccaacg acactcaacc ataccgctgg agtatccagt gacaatcatc cgcgggccgc
    20941 cctctttaac cgtaacttcc tcactaacca taaagctcat actcgcctcc ttttttcgag
    21001 tgaaacttgt tcaccttagt tgaagatggc gaattttgca taatagccag tgcgagtatt
    21061 aatgtgcctg accgcgcatt ttcgctactg ctttcactcc cagcaccacg atgccgccga
    21121 tgataaatcc aagaatcaga tttaaaacag tcggtaatat catcgccacc actgcacttt
    21181 gctgcccggc gatatgttca atagcgtgat gcagcggcgc aataccatgt accacaatcc
    21241 cgccgccgac gaggaacatc gccagcgtgc cgacaatcga taatgctttc atcagccaag
    21301 gcgcgataat caataaccct ttacctaatg cctgcatcag cgcgctagat ttttccgcca
    21361 gccaataccc taggtcatca atcttaacga taacccctac cagaccgtag acgcccacgg
    21421 tcactaccag cgcgatgcct gaaagcacca gcacctgatt aagcaacggc gcctctgcca
    21481 caatccccag cgtgatggcg acgatttccg cagacaaaat aaaatcggta cgaatcgccc
    21541 ctttaatttt gtccttttca aacttcagcg gatcctgcgc cgccagcttc tccagacgct
    21601 gctggctctg cgctggatct tctttatgtt tacgcgcctc cagcatatgc agcactttct
    21661 ctaccccttc aaagcagaga aacgcgccac caatcatcaa cagtggcgta atcgcccacg
    21721 ggataaacgc actgatgatc agcgccagcg gcaccagaat cactttattg atcagcgatc
    21781 ctttcgccac gccccagacc acgggaagtt cccggttggc ccgcacgcct gaaacttgtt
    21841 gcgcattgag cgataaatca tcccctaata caccggcggt tttcttcgcc gccagtttgc
    21901 ccatcacaga gatatcgtcc agcagtgtgg cgatatcatc gagcaacgtc agtaaactac
    21961 tcccggccaa aagcattccc tctcattttt attgttgaag ggtgtaagta tgaagcaaaa
    22021 acggcagctc tgatacttgc ttcacaggtc aacatttatt taacaatcaa gtgcaattat
    22081 atctctcgcc agcagaatga ttttcacgtt tactatgaac caccttttta tttccgccgt
    22141 gagggattat gcgtttccgc cagttgttac cgctttttgg cgcgctgttt gcgttgtata
    22201 tcatttgggg ctcaacctat tttgtcattc ggattggcgt gggaagctgg cctccgttaa
    22261 tgatggcggg cgttcgattc caggcagccg gtattttatt gctggcattt ttgctactgc
    22321 gcggacacaa acttcccccg ctacgtccgc tgctcaatgc cgcgctgatt ggcctgttat
    22381 tgctggctgt cggtaatggc atggtgacgg ttgccgaaca tcaaaatgtt ccttccggca
    22441 tcgccgccgt agtgattgca accgtgcccc tctttaccct gtgcttcagt cgcctgtttg
    22501 gcattaaaac gcgcaaactg gaatgggtgg gtattgccat tgggcttgcc ggaatcatca
    22561 tgctcaatag cggtggaaat ttaagcggca atccgtgggg cgcgattctg attttaatcg
    22621 gctcgattag ctgggcgttt ggctcagttt atggctcgcg cattacctta cctgtaggga
    22681 tgatggcggg tgcgattgag atgctggcgg caggcgtggt gttaatgatc gcatcgatga
    22741 ttgcgggtga aaaactgacg gcgctccctt ccctgtcagg cttccttgcg gtcggctatc
    22801 tggcgctgtt tggttcgatt atcgccatta acgcttatat gtatttaatc cgtaatgtca
    22861 gtccggctct cgccaccagc tacgcctacg ttaacccggt ggtcgcggtc ttgctgggta
    22921 cgggactggg tggcgaaacg ctgtcgaaga ttgaatggct ggcgctcggc gtaattgttt
    22981 tcgcggtggt actggtcacg ttgggaaaat atctcttccc ggccaaaccc gttgtcgcgc
    23041 cagttattca ggatgcatca agcgagtaaa tgaatcccct gcgtgtcgat ctgcgcgctg
    23101 gcaccttcgc cgcagatcca ctcttccaga cgctcggtaa gcgccgcatc cgtcagcttc
    23161 tcgcgcctac gtaacgcaca ctcccagaca atcagtacgc gccagccgag ctcctgcaag
    23221 cgactgatat cgcggcgatc gcgttcaaca tttttaccta tcttctccag ccagaactca
    23281 gttcgagtcg caggcacttt aaacagatag cagtgatgat gatgccagaa gcagccgtgg
    23341 gtaaatatca cgcagcgata ttcatcaacg acaaaatccg ggcgtccggg cagactggcg
    23401 tcctgaacgc gaaacgcaag gccctgcccg gttaacaaac tggcgaggcg cttctcaatc
    23461 gccgtatcac gcgtggcaat cgcgcgcata tttttgctgc gggtggcctt atcgtgaacg
    23521 tcgaccatgt tgtgcctctt gctgacgcaa cgccaccgcc tgtttgattt ttggctcaag
    23581 cagttttgcc accgcggcaa agaccggcac gaccaccgag ttgccgaact ggcgataggc
    23641 ctgagtgtcc gaaaccggaa tgcggaattt cgcttctccc ggcgcttcaa aacccattaa
    23701 gcgcgcgcat tcacgaggcg ttaaccgacg tggacgatgt tgctgattca gcggatcgtc
    23761 aaagtctttc tcacccgtgg ccatatccca accgcgatcg attaaaattt ccgcgccatc
    23821 tttgtaataa cgagcagaca gcgtacgggt gacgctttgc ggattgttcg gataaaccat
    23881 tccataaccg aagccgttac cgcgcgcctg atgttttttc gcatatcgat agaggtactt
    23941 ccacagcacc ggcgtcagga tatatttcgc ctcaaccatc gggtccagca gctgcgccag
    24001 cgtcactcgc tgtgcaggga aacattcgct gatatcacgc agagtaaaat cggctttcag
    24061 attcagatcg cgacgaaaac ccaccagcac gatgcgttca cggtgctgcg gcaggaaatg
    24121 tttaccatcg atgattttcg gatcgtccgg cccgttatct tctgcatcgg caacgtcata
    24181 gcccagttcg tccagcgtct gcatgatgat gcggaacgtt tttcccttat cgtgactctt
    24241 caggtttttg acgttttcga gcacaaacat cgccggacga cgcgcgtcga taatgcgcac
    24301 cacatcaaag aacagcgtgc cctgggtatc gcaggcaaaa ccgtgcgccc gcccgagcga
    24361 gttctttttc gatacgccag ccagcgaaaa tggctgacaa gggaaaccgg ccagtaaaac
    24421 atcgtgttca ggaatgtgtt gacgaatatg ttccgccgcc gcctcatcac tcacgccttc
    24481 ttgatgactg agggtgatgt cgcggatatc ttcattaaaa tgatgcgtcg ccgggtcgca
    24541 ataatggttg gctttataag tgcgtaccgc atgtttgttc cattcgctgg taaacacgca
    24601 ctgtccacca atcgattcaa aaccacgacg gatgccgcca attccggcaa atagatcgat
    24661 aaagcgaaac gcataatgcg gatgatgttc cggtggtttg ggtaacaacg tttgcagatg
    24721 ggcaaactct ttctcactta aacggtgcca tgccgagtca ttcgccagcg cacgttttaa
    24781 aattgccgcg ctccagtgat tctcacccac accattaagc tgcgccacca gcattttaac
    24841 atcataaatt tgcagcagtt tctccagcat tgcctgtgcg gcattcccgg tgctgtatga
    24901 atcggttact gatatatttt cctgcataga tttaaccggc catctgagat ggccgacagg
    24961 ttaacataat tactgccgtt tagggccacg cgctcagcca gcgggtgaaa acgcatctat
    25021 caccttgtga tcgatacctt cattctcccc cgccagttcg gcaccgagct tcgccatcaa
    25081 acagcgccaa gctcaatgcc cctgaaacgg caaatacgcg cgccaggcca atcgccccca
    25141 gggcttccag ccgatccgca tcctggacga ttttagcttc cgttgttaag ggggcgattt
    25201 gcgcgctgaa actgtgagcg gcaatggcat gacaaacggc ctcaattttc tccgccggaa
    25261 attgcacgaa ctcttcacgg agcaggcgac gcgtctcttc tgccgccagg attgaagaac
    25321 gctgccgttg cggatgattt ttcgccaggc tcacgatatc gtgaaaataa caggcggtta
    25381 aaatcaccag catatcgaca tcgtcatctg ccgccagttt ctgtgccgta gcccagacac
    25441 ggcgaaaatg acacaggtca tgcgccgcgt cctgatgttg atgatggttt ttcaaccagt
    25501 tctcgaacta tgcctgccag tgttgtaact ccatacgcgc tccgttgcca aagagtctca
    25561 accttagcag tttttattca tagcgtctgc aacgtgcggg acgaggtcgg gtgtaccatg
    25621 caatgccgcc taacaaggca ccaaccaggg ccataaacag aaatccgacc aatgctccta
    25681 accacttccc cgcggtagat cctaaatcac tttcaaatcc cgacacgggg gcatcgggga
    25741 tccggctcat cacccagatg tatcgcgcca ttgcccagac gaaatagcca caccatgtat
    25801 agaaagccc
//
LOCUS       DADLTY010000045        25755 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.045, whole genome shotgun sequence.
ACCESSION   DADLTY010000045 DADLTY010000000
VERSION     DADLTY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.045"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="JN334_001566"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="JN334_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HAY7437740.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="JN334_001567"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="JN334_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HAY7437741.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="JN334_001568"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="JN334_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HAY7437742.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..5082
                     /locus_tag="JN334_001569"
     CDS             4843..5082
                     /locus_tag="JN334_001569"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437743.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5092..5262
                     /locus_tag="JN334_001570"
     CDS             5092..5262
                     /locus_tag="JN334_001570"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY7437744.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5311..6303
                     /locus_tag="JN334_001571"
                     /pseudo
     CDS             5311..6303
                     /locus_tag="JN334_001571"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            6355..6990
                     /locus_tag="JN334_001572"
                     /pseudo
     CDS             6355..6990
                     /locus_tag="JN334_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216201.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
     gene            7084..7773
                     /locus_tag="JN334_001573"
     CDS             7084..7773
                     /locus_tag="JN334_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7437745.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="JN334_001574"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="JN334_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HAY7437746.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="JN334_001575"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="JN334_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HAY7437747.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="JN334_001576"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="JN334_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HAY7437748.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="JN334_001577"
     CDS             complement(10475..12193)
                     /locus_tag="JN334_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY7437749.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="JN334_001578"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="JN334_001578"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HAY7437750.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="JN334_001579"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="JN334_001579"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HAY7437751.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="JN334_001580"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="JN334_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HAY7437752.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="JN334_001581"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="JN334_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HAY7437753.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="JN334_001582"
                     /pseudo
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="JN334_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18432..18746
                     /locus_tag="JN334_001583"
     CDS             18432..18746
                     /locus_tag="JN334_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HAY7437754.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="JN334_001584"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="JN334_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HAY7437755.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21862
                     /locus_tag="JN334_001585"
     CDS             20570..21862
                     /locus_tag="JN334_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY7437756.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22016..23665)
                     /locus_tag="JN334_001586"
     CDS             complement(22016..23665)
                     /locus_tag="JN334_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HAY7437757.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23816..25165)
                     /locus_tag="JN334_001587"
     CDS             complement(23816..25165)
                     /locus_tag="JN334_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7437758.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
BASE COUNT         6407 a         6967 c         6624 g         5757 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcactgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttgttat gggaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcgatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtaaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcactag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    19561 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
    21361 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
    21421 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
    21481 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
    21541 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
    21601 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
    21661 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
    21721 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
    21781 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
    21841 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
    21901 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt
    21961 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
    22021 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
    22081 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
    22141 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
    22201 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
    22261 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
    22321 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
    22381 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
    22441 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
    22501 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
    22561 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
    22621 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
    22681 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
    22741 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
    22801 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
    22861 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
    22921 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
    22981 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
    23041 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
    23101 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
    23161 ctgccacggc aaacttcaac gacaccaggc cggaagcgtc attcatcaac gcttcgccct
    23221 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
    23281 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
    23341 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
    23401 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
    23461 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
    23521 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
    23581 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
    23641 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
    23701 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
    23761 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
    23821 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
    23881 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
    23941 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
    24001 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
    24061 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
    24121 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
    24181 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
    24241 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
    24301 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
    24361 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
    24421 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
    24481 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
    24541 tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg
    24601 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
    24661 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
    24721 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
    24781 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
    24841 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
    24901 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
    24961 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
    25021 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
    25081 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
    25141 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
    25201 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
    25261 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    25321 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
    25381 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
    25441 atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt
    25501 ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg
    25561 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt
    25621 gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc
    25681 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa
    25741 agagctcgat cagtc
//
LOCUS       DADLTY010000046        25716 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.046, whole genome shotgun sequence.
ACCESSION   DADLTY010000046 DADLTY010000000
VERSION     DADLTY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.046"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..915)
                     /locus_tag="JN334_001588"
     CDS             complement(<1..915)
                     /locus_tag="JN334_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAY7437759.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRV"
     gene            1210..1884
                     /locus_tag="JN334_001589"
     CDS             1210..1884
                     /locus_tag="JN334_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7437760.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(1923..3584)
                     /locus_tag="JN334_001590"
     CDS             complement(1923..3584)
                     /locus_tag="JN334_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HAY7437761.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(3780..4208)
                     /gene="ibpB"
                     /locus_tag="JN334_001591"
     CDS             complement(3780..4208)
                     /gene="ibpB"
                     /locus_tag="JN334_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HAY7437762.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(4320..4733)
                     /gene="ibpA"
                     /locus_tag="JN334_001592"
     CDS             complement(4320..4733)
                     /gene="ibpA"
                     /locus_tag="JN334_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HAY7437763.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            5038..5370
                     /locus_tag="JN334_001593"
     CDS             5038..5370
                     /locus_tag="JN334_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAY7437764.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(5372..6646)
                     /locus_tag="JN334_001594"
     CDS             complement(5372..6646)
                     /locus_tag="JN334_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HAY7437765.1"
                     /translation="MMAGTVLYQDRAMKQITFAPRNHLLTNTNTNTNTNTNTWTPDSQ
                     WLVFDVRPSGASFTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGP
                     ENPDETWHYDFHHRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGS
                     FTYNDHVMHQLDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPG
                     SDEINRAYEEGWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGT
                     ETTLPAPPRGVVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLW
                     LISPQGGEPRKLTHNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHA
                     NPPSADAVVFSPDGQWLAWMEGGQLWITETDR"
     gene            6711..7811
                     /gene="cbrA"
                     /locus_tag="JN334_001595"
     CDS             6711..7811
                     /gene="cbrA"
                     /locus_tag="JN334_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HAY7437766.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(7772..9109)
                     /locus_tag="JN334_001596"
     CDS             complement(7772..9109)
                     /locus_tag="JN334_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7437767.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(9184..10332)
                     /gene="dgoD"
                     /locus_tag="JN334_001597"
                     /pseudo
     CDS             complement(9184..10332)
                     /gene="dgoD"
                     /locus_tag="JN334_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(10329..10946)
                     /locus_tag="JN334_001598"
     CDS             complement(10329..10946)
                     /locus_tag="JN334_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HAY7437768.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(10930..11808)
                     /locus_tag="JN334_001599"
     CDS             complement(10930..11808)
                     /locus_tag="JN334_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HAY7437769.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(11805..12494)
                     /gene="dgoR"
                     /locus_tag="JN334_001600"
     CDS             complement(11805..12494)
                     /gene="dgoR"
                     /locus_tag="JN334_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HAY7437770.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            12772..13428
                     /locus_tag="JN334_001601"
     CDS             12772..13428
                     /locus_tag="JN334_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437771.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(13474..14286)
                     /gene="yidA"
                     /locus_tag="JN334_001602"
     CDS             complement(13474..14286)
                     /gene="yidA"
                     /locus_tag="JN334_001602"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAY7437772.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(14400..14807)
                     /locus_tag="JN334_001603"
     CDS             complement(14400..14807)
                     /locus_tag="JN334_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HAY7437773.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(15038..17452)
                     /gene="gyrB"
                     /locus_tag="JN334_001604"
     CDS             complement(15038..17452)
                     /gene="gyrB"
                     /locus_tag="JN334_001604"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAY7437774.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(17481..18554)
                     /gene="recF"
                     /locus_tag="JN334_001605"
     CDS             complement(17481..18554)
                     /gene="recF"
                     /locus_tag="JN334_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAY7437775.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(18554..19654)
                     /gene="dnaN"
                     /locus_tag="JN334_001606"
     CDS             complement(18554..19654)
                     /gene="dnaN"
                     /locus_tag="JN334_001606"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAY7437776.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(19659..21062)
                     /gene="dnaA"
                     /locus_tag="JN334_001607"
     CDS             complement(19659..21062)
                     /gene="dnaA"
                     /locus_tag="JN334_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAY7437777.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            21359..21439
                     /locus_tag="JN334_001608"
     CDS             21359..21439
                     /locus_tag="JN334_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437778.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            21669..21809
                     /gene="rpmH"
                     /locus_tag="JN334_001609"
     CDS             21669..21809
                     /gene="rpmH"
                     /locus_tag="JN334_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAY7437779.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            21826..22185
                     /gene="rnpA"
                     /locus_tag="JN334_001610"
     CDS             21826..22185
                     /gene="rnpA"
                     /locus_tag="JN334_001610"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAY7437780.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            22149..22406
                     /gene="yidD"
                     /locus_tag="JN334_001611"
     CDS             22149..22406
                     /gene="yidD"
                     /locus_tag="JN334_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAY7437781.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            22409..24055
                     /gene="yidC"
                     /locus_tag="JN334_001612"
     CDS             22409..24055
                     /gene="yidC"
                     /locus_tag="JN334_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAY7437782.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            24161..25525
                     /gene="mnmE"
                     /locus_tag="JN334_001613"
     CDS             24161..25525
                     /gene="mnmE"
                     /locus_tag="JN334_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAY7437783.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6236 a         6937 c         6544 g         5999 t
ORIGIN      
        1 tacccgattt tccggtgcgg cagtgaagta catcgctaaa gaaatgccca cggcaccaaa
       61 gatttttgag ttaccgtgca gggcaaaacc gccttccggg aacaacgatt tcagtggctc
      121 ggcgctcaga ctgaactctt gcagatgctg cgcccagtac atctgaatgc cgccttcaac
      181 agctgccgga ccaaagataa acggtccgta gatgaagtgg tgtaaaccgg ttgggatcag
      241 aatacgttcg aggaaggtgt aaacccacac cccaagcgca cccgccgaac gcaggaacgc
      301 ttgcagagat tcaatcccca tttgtacttt tggccagccg agcaatgtca gccaggcaca
      361 ggggatcatc accaggaagg cgataatcac cacataagac gtcccctgga aaatgccgag
      421 aaaaaccggc agttttttat cgaacagacg gttatgcagc gccgtcacaa tgccggaaat
      481 gataattgcg ccgataatgc tggtatcgag ggttttaatc ccggccatca ttgtcagacc
      541 gctacctgcc accgcgtcct gagtgaaatc gacgccgaag tagcttcccc aggtcattcc
      601 catcgcattg atgaaatagt tccaggtcag gaaactcacc atcaccgcca gacaagcacg
      661 tccctgcgct tgcttagcaa ggccaatggg taaaccgaca gcaaaaatca gcggcatatt
      721 acggaatacc gtccaaccgc cctcttcaat aatgtgtacg atttgcgcga ataaactgtt
      781 cggatcggtc agtgattccc cgacaaacat cgggttttgc agcaagatgg caagacccac
      841 cacaatcccg gcgaagggaa acagcagcac tggcgtgaac atcgcgccgc caaagcgttg
      901 aatttgactg agcatttcca catcctcttt tctcaattct gtaagtagga agcctgattg
      961 gttatgcggc agacctggag cgaggatagg aagatgcgat cggcagaaca tgacgaccca
     1021 atgggattcg tgatcgcttt catgcttttc gttgggcggt agagatctac gttttcgcga
     1081 aaaagtagac atctgtgagc acaggtacac agcatcgaac acatctttaa aaaaaagatt
     1141 tttttcaatc gattaagcag aacttgtggg cgcattaccc gtgcttgcag gcaaaaaaga
     1201 gagctagaga tgatctacaa aagcattgcg gagcggttaa gaattcgact taattccgcg
     1261 gatttcacgc taaacagcct tcttcccggt gaaaaaaagc tggcggaaga gtttgcggta
     1321 tcgcggatga ctatccgtaa agccattgac ctgctggtag cgtgggggct ggtggtccgc
     1381 cgccacggca gcggcactta cctggtgcgc aaagatgtgc tgcatcaaac cgccagcctg
     1441 accggactgg tggaggtgtt aaaacggcag ggaaaaacgg tcaccagcca ggtgctgatt
     1501 tttgaaatca tgcctgcgcc tccggccatt gccagccagt tacggattca aatcaacgag
     1561 cagatctact tctcccgtcg cgttcgtttt gtggaaggga aaccgctaat gctggaagac
     1621 agctatatgc cggtaaaact gttccgtaac ctttcgctgc aacatctgga agggtcgaag
     1681 tttgaatata ttgaacaaga gtgcgggatt ttgattggcg gtaattatga aagcctgatg
     1741 ccggtgctcg ccgatagact gctggcgcgg caaatgaagg tagcggaaca cacgccactg
     1801 ctgcggatca cctcgttgtc atatagcgag agcggggagt ttttgaatta ttcagtgatg
     1861 ttcagaaatg ccagcgaata ctaggtggag taccatttac ggcgactcca cccggaaaaa
     1921 agttaaccga tactccagaa gagcaccgcc agtaattggg gggtgataat gcgcaggaac
     1981 atcactaacg gatagacagt ggcgtaagag agcgccgccg caccactggt tggatgaaga
     2041 ttattagcaa acgccagcgc cggaggatcg gtcatggaac ctgccagcat cccgcacatg
     2101 gtcaggtaat tcattttggc taacatccgc gccagaatgc caacagtaat cagcggaacg
     2161 gcggtgatca gggcaccata accaatccag cttagccctt cgccattgac cagggtattc
     2221 acaaaatccc caccagattt cagaccaacg accgagagga acagcacgat ccccagctcc
     2281 cgcagcgcga ggttggcact tggcggcata aaccagtaca gcttgccgat actgccgata
     2341 cgcccgagga tcaacgccat aatcagcgga ccgcccgcca gccccagttt caacgcggcc
     2401 gggaatcctg gcacaaagac gggaatagaa cctaacaata cgccaagccc gatgccaata
     2461 aacactggca gcatctgaac ctgttgcagt ttttgctgcg cattccccag cacattggca
     2521 acggcatcaa ttgcggacgg acgccccacc aggttgagga tatcgccgaa ctgcaggctg
     2581 atatcgccac tggcgaccag ttcgaccccg gcacggttca ggcgcgagat aacaacgtca
     2641 tagcgttctt taaagtgcag gtcgcgaata cgttttccga gcacgttttc attggtgacc
     2701 accacacgct cgacgcgcaa atcagtgcct ttcgtagaca gcgaggtatc gacctcctga
     2761 ccaatcacca gttgcgcatt atgtaaatcc gctggctgcc ccaccagatg cagcaaatcg
     2821 cccagttgga taatggtatc tggcgaaggt acttttaggg tttcttctcg tttcagacgc
     2881 gagcagataa ttttgtcgcc gttgagaatc ggtacatctt taatggctaa atcatgcagg
     2941 ttagggttct caacgcgaat attgatagtc ttgatcagcg cgccgccatt ggtgcgtgaa
     3001 gactcgtgct gctgagcttc tgtctcgaca ttgacgcgga aaataacccg caacatccac
     3061 atggtgaaca aaatcccgca aatgccgaat ggatacgcca tcgcgtaact catccccatc
     3121 tgatcgacca tttccattgg tgtacccagg tcgcgcaaga tctgctgccc tgcccccagc
     3181 gctggcgtat tggttaccgc accggagaaa atccccagca ctaccggcag tggaatatca
     3241 aacagtttat gcaggatggc ggtaaccaga ccaccgatga tgacgatcag aacagcaaac
     3301 aggttgaggc gtaatccgga gacgcgcaat gaggcaaaga agcccggccc tacctgaatc
     3361 ccgatggtat aaacgaacag gatcaggcca aattcctgaa taacatgcag catatcgcta
     3421 cttaatgtca tccccgcctg agaaacaaaa tggccgacga tgatcccacc aaacagcacg
     3481 ccgccaatac ctaatcctat gccgcgaaat ttgacgttgc cgataaacaa accgacgact
     3541 gccaccaaag ccagaatact gaccgttaat gctatatcac tcatcgtcca tttctcttga
     3601 gataacattt taattgctac ggattatggc tgaatcggtg aagcagattt gccttatagc
     3661 gcacattatc acgggaaaga acgtgccgaa atatcttaaa cagtcgaaat ttaacgcatt
     3721 gttgaagaag aaagacaaaa aaagccccac cagaatggcg gggcaaagag aatagctagt
     3781 tagctattta acgcgggacg ttcgctgata gcgatacgct gcgctgcgat gggttcaggc
     3841 tcattacgaa ttaaatcaat atgcagtaaa ccgtttacga aggttgcgcc agagacttcc
     3901 atattttcag ccagcgtaaa actcaggcta aatggctgat tcataagccc ttgatgcagc
     3961 cattttttct cttcttttgg ctgctccggc gtgcctttta cgctcaggcg cgtgccttcc
     4021 agttgaatct ctaaatcttc ctgacggaaa cctgccagcg caagggtaat gcggtagtgg
     4081 ttatcgtcgc ttttctcaat gttgtacggc gggaagctct ggctttcacc ggcgttttgc
     4141 agtgcgttgg ccagtttgtc aaaaccgatc cattgacgca tcagtgggga taaatcgaag
     4201 ttacgcatag tcatttctcc ttctaagaag cgagtaagta cctgcaaatc cgaagattcg
     4261 catatgctcc ctgacggcga gcatggagat gtcaggccgc gccaggcggc cttagggaat
     4321 tagttgattt cgatacggcg cggttttttc gcttccggaa tcacgcgttc gagatcgata
     4381 tacagcaaac catttaccag gttagcccca cgaacatgaa tgttctcagc taactggaat
     4441 ttgcgttcaa agttgcgttc agcgatgccc tggtacagat aggtgcgctc tttttgttcg
     4501 tcggcgtgag cacctttcac caccagcaga ttatcctggg cggtaatttc cagttcgctc
     4561 tcagcaaaac cagccacagc gatagcaatg cggtaatggt tttcgtctac cagttcaacg
     4621 ttatacggag ggtagccgcc attactctgg ctctggttgt tttctaagtg gttaaacaaa
     4681 cggtcaaatc caatagcaga acggtaaagc ggggataaat caaagttacg cataatcaat
     4741 agctcctgaa atcagcgaga atgtaagacc ttccacaatg gacaggtcag gtagccagaa
     4801 cacccatcag gcgcgtcctc atcgactacg atgtaaaatg ggtctggaaa tgaacttttc
     4861 aagccttatc agcggacttt tttttgcagt ttatggtcta ttgcatagac tgagggggca
     4921 gcacgaaacg ttaaagtgcg atagccgcca cagtgcgacg taatgatgat gttattttca
     4981 gcaagggatc gctataactc atcaggccaa cacccacgat caacagatga atttattatg
     5041 ataagaaatg tgttgttagc gttcatgata tgcagcggaa tgacattact cggaggatgc
     5101 tccagcgtga tgtcccatac cggggggaaa gaaggaacgt atcccggcac ccgcgccagc
     5161 gccaccatga ttggcgacga tgaaaccaac tggggcacta agtcgctggc gattctcgat
     5221 atgccgttta ccgctgtaat ggacacgctt ttactgccgt gggatgtgtt ccgcaaagac
     5281 agctcggtgc gctcgcgcgt tgaaaaaagc gaagccaacg cgcaagccac caatgcggtt
     5341 attccaccgg ccagaatgcc tgataactga tttagcgatc agtttcggtg atccacagct
     5401 ggccaccttc catccacgcc agccattgac catccggcga gaagaccacg gcgtccgcag
     5461 aaggtggatt ggcgtggttt tcggttaaat actcaacctc gccgctttgc gcatgggcac
     5521 aaacaattcg attatccagc acaaagccca accattctcc tgacggatgc cagttaaatg
     5581 cagactgaat atccgttttg ttatgggtta acttgcgcgg ttcgccgccc tgtggcgaga
     5641 taagccacag ttgcacaatg ccgttatcat cacgcattaa aaacgcgatt tgcgtaccct
     5701 gcggattaca gcgcacccag tggcgcggga cgttgactaa ccccggataa gcccgatgat
     5761 gggtaaaggt taaacgtcgc tgcacgacgc cacgcggtgg cgcgggtagg gttgtttccg
     5821 ttccacttaa cggcgcatca cctgccgatt tccagctagc ttcatcttgc ggtaactcaa
     5881 cgataaatag ctccggcact ttctcgccct ttggcgaaag tgtgtcgcca ataaacgcca
     5941 gcgcgtgatt tcctacccat ccttcttcat aagcacgatt gatttcatca ctgccaggct
     6001 gcggcgtggg cgtggtttta ctcaccagca cacaccagtg gctaccgctg tattcacgcg
     6061 gatgttgctt ttgtacgttg accgggccaa acggtgcagc aaccccgacg tttcgcaaat
     6121 ccagcgccgg atcgagttga tgcattacat ggtcgttata ggtaaagctc cccctttcac
     6181 cgttcgggct aaagacatgc acatggctgc caccgcgcag cgctcctggc gtatacggcg
     6241 cggtaatatc cattgcatcg agattgctca ccttgccgcc ttcagcaatg actccgcgtc
     6301 gatgatggaa gtcgtaatgc catgtttcat caggattttc agggccgtga ataaagacat
     6361 atttctctga cttcggatga acggtcacca cgccgacgtg tgcgccctgt gacgcgcgat
     6421 agataacctc gacctcgcct gtatggatat tcacacgctc aatggtctcg ccggtaaacg
     6481 acgccccaga aggacgcacg tcaaatacca gccactggct gtcgggcgtc caggtattgg
     6541 tatttgtatt ggtattggta ttggtattgg tgagcaggtg attacgggga gcaaaggtta
     6601 tctgtttcat ggcgcggtcc tgatacaaaa cggttcccgc catcatactt cacaaggact
     6661 tcactttcag acaatagtct tttgccatcg tgatgaatgt gagtgaaaag atggaacatt
     6721 tcgacgtggc gattattggc ctcggcccgg cagggtcggc gttggcacga aagttagccg
     6781 gcaaaatgca ggtgatcgcg ctggataaaa agcaccagtg cggtactgaa ggtttcagca
     6841 aaccttgcgg cggtctgctg gcaccggacg cgcagcgatc ttttattcgc gatggactga
     6901 cgcttcctgt cgatgtgatc gccaatccac agattttcag cgtcaaaact gtcgacgtcg
     6961 ccgcatcgct cacgcgtaac taccagcgaa gctatatcaa tattaatcgc catgctttcg
     7021 acttgtggat gaaatcgctg atccccgcca gcgttgaggt ttatcacgat agcctgtgcc
     7081 gaaaaatctg gcgtgaggat gataaatggc atgtcatttt tcgtgcagac ggctgggagc
     7141 aacatattac cgcccgctat ctggtcggtg ccgatggcgc aaactcgatg gtgcggcgac
     7201 atctctaccc ggatcatcaa atccgtaaat atgtcgctat ccagcagtgg ttcgcagaga
     7261 aacatccggt gccgttctac tcctgcatct ttgataatgc gataactgac tgttactcat
     7321 ggagtatcag caaagatggt tattttatct ttggcggtgc ctatccaatg aaagacggtc
     7381 agacgcgttt cacgacgctg aaagagaaaa tgagcgcctt tcagttccag tttggcaaag
     7441 cggtgaaaag tgagaaatgc acggtgttgt ttccctcacg ctggcaggat tttgtctgtg
     7501 gtaaggacaa cgcctttctg attggtgaag cggcgggatt tatcagcgcc agctcgctgg
     7561 aggggattag ctatgcgctg gatagcgcag agattctgcg tgcggtgttc ctgaaacagc
     7621 cggagaagag caacgccgct tactggcgcg ccacacacaa actgcgttta aaactcttcg
     7681 gcaagatagt aaaaagccga tgcctgaccg caccggcttt aagaaagtgg attatgcgca
     7741 gtggtgtggc gcatattcca cagttgaaag attatccaac gcgcttcaca tcacccacca
     7801 gcaggatgta agagagcgcg ccaatcaacg cgacggcgga gatataaacc agtgcaggcg
     7861 cgaaaccgta accctgcgcc aggtacccca ccaccagcgg aacggtgatg ccgcccagac
     7921 caccggcgaa gttaaacacg ccgccggtta aaccaatcag gcgcatcggt gccagagaag
     7981 agaccagcga ccaggtaatc gaagcaaaac cgttgccgaa gaatgccagc gccatcaggc
     8041 acataatcat catcggatcg ttggtgtagt tagcgcccat aatgcaggtg gagatcagca
     8101 agccgcagat aatcggcgtt ttacgcgcaa agcccagtga aaagccctta cgtaccagca
     8161 gatccgcgac ccagccagag agcaggacgc cgacaaacgc cgcgaggaat ggcaccgtgg
     8221 tcataaagcc tgctttcagc gccgtgattc ctttttcctg ggttaaatag ttcgggaacc
     8281 aggttaagaa aaaccacagt gcagaagcca ccgcaaattg cccaagataa acgccgatca
     8341 gtttacgatg gaacaccagt ttccagtctt tggctgttaa cggctgacgc gcctctttct
     8401 tcaccggcgc atcaccatcc accagaccgc cgccatcacg aatgtaatcc agttcagctt
     8461 tgctgatacc tttggtcagg cgcggcggct gataaacctt aaaccaaatc agcgaccaga
     8521 taatgccgat accaccagtg acaatgaaca cccagtgcca gctcaacatc tcctgaatcc
     8581 agatcagcag cggagtcaaa aacgccagac cgacaaactg accagacgta taaaaaccaa
     8641 cggcggaagc gcgttcatgt tccgggaacc agctggtcac catccggtta ttggtcggga
     8701 aagcgggcgc ttcgaaaata ccggttatcg cacgcagacc aattaatgac attaatcccg
     8761 tggcaaagcc ttggaataaa gtcgccactg accaaccaaa tatcgcaata aaataagtca
     8821 cgcgagaacc tacgcgatct aaaaaccaac cgccggggat ctggcatagc gtataaagcc
     8881 aggcgaaggc cgaaaataca tagcccattt ccgctttcgt aatgccgaac tcttcctgaa
     8941 tatgggcgga agccacggcc aggttagcgc ggtcaacata acaaatgacc accgtaataa
     9001 agatcatcac cagcgtcaga taacgccgac gccccggctt tgctgcatta acgggaatat
     9061 ccatagcgag ctttctccag attttgggca tagcgaagcc gctcaccatg ccctgtaatt
     9121 tacagagggt tatatttttg tattgctgtt ttagtgcccg atgaggggct tacgtggcag
     9181 gaattaccac tctgctacgc tgttatcttc atgacgccag agcggattac gccagtccgg
     9241 ggcattttta ctgaactcaa taactttagc ttcgtcgatt tccacaccta agcccggttt
     9301 cgttaacggt ttaaagaagc cgccaaccat gctgaagtct tctttgtttt tcacaaagtc
     9361 gagtaactcc gcgcctttgt tgtaatgaat gcccatactt tgttactgaa gtaccgcgtt
     9421 ataggaaaca aagtcgatat gcaggcaagc cgccagtgca atcggtccga acggacagtg
     9481 cggcgcaagg gtcacatcat aggcttctgc cattccagca attttgtagc attcggtaat
     9541 accgcctgca tgggagagat ccggttgcag aatcgaaata ccgcctgcct ccagcacgcg
     9601 tttaaaatcg aaacgtgaga acatgcgctc acccgccgcc agtggaatat gcgtttgtgc
     9661 cgccaatttc gggtagtatt cggcttgttc cgccagcacc ggctcttcaa taaacagcgg
     9721 gcgatacggc tccagttctt taatcagcac tttcgccatt ggcgcgctga cgcgaccgtg
     9781 gaaatcaaga ccaaactcaa tctgattgcc aaaagcttca cgaatttgtg ccacggtgtt
     9841 gactgccgca tctaccgcgc gggagttatc aattagcccc agttcttcac aaccgttcag
     9901 tttgaaggta tcgaagccga tttcgcgcag ggttttaatg ccgtcgataa catccgccgg
     9961 acgatcgccg ccgacccaac tgtaggcttt aattttgtcg cgaaccaggc cgcccatcag
    10021 ttgccagact ggcgcattca gcactttgcc tttgatatcc cataacgcct ggtcgatccc
    10081 ggcgatggcg ctcatcagga ttggaccgcc acgataaaat ccggcgcgat acatcacttg
    10141 ccataagtcg ttgatgcgcg aaggatcctg accaatcaaa tagtcaccca gctcgtgaac
    10201 tgccgcttcc accgtacggg cgcggccttc aatcacgggc tcgccccaac cgaccacgcc
    10261 ttcatcggtt tcaattttca ggaacatcca gcggggaggt aaacgatacg tggtaatttt
    10321 ggtgattttc attgcactgc ctctcgatac gccttaacaa atgctgctgc ctgctgcgcg
    10381 gtgcgttcta cggactgccc ggcgcgataa agatcgctgc ctaagcccgc cccaacacaa
    10441 cctgcgtcta tccactgcgc caggttttct ggcgtcacgc cgccaacggc aaagactgcg
    10501 atgtccggtg gtaataccgc ttttaacgct ttgatgtatt gcggaccaaa agccgatgac
    10561 ggaaatattt tcagcgcctg cgcgcccgct tcgagcgcgg taaaggcttc ggtcgccgtc
    10621 gcgcagccgg ggcaaacggt catgccgtag cccaccgcgc ggcggatcac ttcactatgg
    10681 atattgggcg taacgatgag ctgacagccc atcctggcga gcgcatcgac ctgttcaggt
    10741 ttcagtaccg tacctgcgcc aatcaacgcc ttgtcgccat acgcatcaac gatggcggga
    10801 atgctctgct cccattgtgg ggaattcagc gggatttcaa ccgcgtcgaa cccggcgtca
    10861 atcaccgcgc caacatgcgc cagcgcctcg tcgggcgtaa tgccgcgcaa aatggcgatc
    10921 agcgggagtt tagtttgcca ctgcatgagc gatgctcctt ataccagcct gaaatgccgt
    10981 gtcgcccgcc accgccgcca cgtcgcaacc catcgcctga aaggcttgct ggtagcgcgc
    11041 ggtcagcgat gttccggcga caagggtgat ggcgtgttga tgggtcacat agtcgcgcat
    11101 actggcaacc tctgcgccaa tcaacaaacc agagagaaat tcgctgacct gttcgcgggg
    11161 aagtgttccc agcacatgcg aggcgcgaac ttcaaaaagc tgcggcaata tggcgggcgc
    11221 attaaggccg cgctcaaggc cagccgcgaa ggcatcggca gagttttcct gcggcggcaa
    11281 acctgcgcca atcaatgagt gatttaacag taaatgatgt aattcaccgg tcatcacggt
    11341 gcgaaaatcg ttgatttgct ggctatcggc ctgcacccat ttgcaatgcg taccgggcat
    11401 aacataaaga gaggaaggag ccagagctcg cgcgccgatc aattgtgttt cttcgccgcg
    11461 catcacattg tggttatcgt catgagagac gcataatccg ggaataatcc agatattgtc
    11521 gccaacagac gttaattgtt cgccaataga cgaaaaacgg gcaggaacag ataaatacgg
    11581 agcaactttc cagccgacat tgctgccgac cattcctgcc atcaccactg gcgtattttc
    11641 ttcacgccag tcggtcgtga cttctgctaa cactgcagcc ggagattttc cgttcaggcg
    11701 cgtgacgcct gcttctgatt gcctgctctc caggcagtgg tcgccctgat aaagccaggc
    11761 gcgcagattg gtcgatcccc agtcaattgc gatgtagcga gctgtcatgt gatttccttt
    11821 aaccttcgtg tcgagctggc aatcatggta agcgccgcct gctctgccgc atcgccgtcc
    11881 tgatgccgta tcgcatcgaa cagcgcctta tgttcctgga gcgtttgcgg catgttggcc
    11941 tcatcgccca tccaggttcg ttcaaaaacc gcccgctgca gcgaactgat cgcaatgcta
    12001 agttgctgta acaccgggtt atgcaccgac tgcagcaccg cctcgtggta gcgaatatcc
    12061 gcttcgttaa acgcttcgcg gtcctgattg ttggcaatca tctcgttcag cgccgattca
    12121 atctgcgcca gatcgctgga ggtcgcgcgc tctgctgccc aacgggcaat cgccggttcc
    12181 accagatttc gcacttcgct catggcactg ataagccgtg ggtcgtagtc gttttccagc
    12241 acccattgca gtacgtcagt gtcgaggtaa ttccactggt tacgcggtgc cacaaacgcc
    12301 ccgcgataac gtttcatttc aatcagccgc ttcgccatca gcgaacggaa cacctcacgg
    12361 atgatgttgc gcgaggttgc aaactcttca cagagctccg cctcagccgg aagtggcgag
    12421 ccaggtacgt atttgccgtg aacgatctgt ttacccagcg taatgacaat gcgatcggtt
    12481 ttattgagag tcatggagag tccttgtgct ctgtatgttc ttctctactt taccccgatc
    12541 gatgcataac gcggcaactt tgtagtacca gcgtgatgac gttcgcgttt gccgcgcgca
    12601 taatgtagta caaacttata ttgttgcgct acaatttaga tcaaaaaaag aacaacacat
    12661 aaaaaaatga catgcgtcgg gcagaaatct gaaaagggat atcaggcgct aaacaggagg
    12721 gaaagaagag tatgctttca acggcttagc tactcgttta aaggattaat catgaagttg
    12781 aattttaagg gattttttaa ggctgccggt ttattcccac tggcgctgat gctttcaggc
    12841 tgtatctcgt atgctctggt ttcccatacc gcaaagggta gttcaggaaa gtatcaatcg
    12901 cagtcagaca ccatcactgg gctatcgcag gcaaaagata gtaatggaac aaaaggctat
    12961 gtttttgtag gggaatcgct ggattacctt atcactgatg gtgccgatga catcgttaag
    13021 atgctcaatg atccagcact taaccggcac aatattcagg ttgccgatga cgcaagattt
    13081 gttttaaatg cggggaaaaa gaaatttacc ggcacaatat cgctttacta ctactggaat
    13141 aacgaagaag aaaaggcact ggcaacgcat tatggttttg cctgtggtgt tcaacactgt
    13201 accaggtcac tggaaaacct aaaaggcaca atccatgaga aaaataaaaa catggattac
    13261 tcaaaggtga tggcgttcta ccatccattt aaagtgcgat tttatgaata ctattcaccc
    13321 agaggcattc cggatggtgt ttccgcagca ttactgccag tgactgttac actggacatc
    13381 attactgcac cgctgcaatt tctggttgta tatgcagtaa accaataatc agtaagcggg
    13441 caaacgcgtt tatgctgttt gcccgcccac agattaattc agcacatact tctcaatagc
    13501 aaacgccacg ccatcttcaa ggttggattt tgtgacaaag ttcgccactt ctttcaccga
    13561 aggaatagcg ttatccatcg ccacaccgac gcctgcatat tcaatcattg cgatgtcgtt
    13621 ttcctgatcg ccaatcgcca tgatttcttc cggtttaata cctaacacgt cggccagtga
    13681 tttcacaccc gtacctttgt taacgcgttt atcgaggatt tcgaggaagt acggcgcact
    13741 tttcagcacg gtatatttct ctttcacttc ctgcggaata cgcgcgatag cctggtcgag
    13801 gatggcgggt tcatcaatca tcatcacttt caggaactgg gtattggggt ccattttctc
    13861 cgcttcgcag aacaccagcg gaattgtggc aacgaaggat tcatgcaccg tgtagtagct
    13921 gatatcgcgg ttagcggtgt acagcgtggt gcggtccagg gcgtggaaat gagaaccgac
    13981 ttcgcgagag agtttttcca ggaaacgata gtcgtcatag ctgagagcgg tttgcgccac
    14041 ggtgctacca tcagcggcct tctgtaccag cgcgccgtta taagtaatgc agtagtcgcc
    14101 cggctgttcc atatgcagct ctttcaggta gttgtgcaca cctgcatacg gacgacccgt
    14161 cgttagcacg acattcacgc cacgggcgcg agctgcggca atcgcatttt taacggcggg
    14221 tgaaatggtg tgatcgggca gcagaagggt gccatccata tcgatagcaa tgagtttgat
    14281 agccatgagt tcccaaggta gattagttcc tgacccatgc taacgcgatt ccgctcaaaa
    14341 atcagtacaa cacccgaggg aaaaggggat gcaacgcggg ttgcatgctc cccatttgct
    14401 tagcggaaga gtttcccttt cagcagttcc atgcctgcgg aaagcagatc gttattggct
    14461 tgtgctgaca cttcaccttg cggtgagagc gcatcaataa tcttcggcaa ttgctctgcc
    14521 agtaaactgg aagccgtact ggtatccacg ccaagttttt gcccgagatc ggacaccgca
    14581 tttgtgccga gcgccgactc cagttgctcg ccgctaactg attgatttct ctgttgatta
    14641 ctcaaccagg ttgagagaat ggcccctaag ccgccacttt gcagtttttc cagcagcacc
    14701 tgaatgccac cctgctcctc aacccaactt aaaatcgcct gatatttccc cgcatcgcct
    14761 ttcagaaagg caccgacaac ttcatcaaaa agccccatga taatcacctg taaagcgtta
    14821 cgtgttgacc caaaaagtat agatctgcgg gatgataatt gcggattgca gaaataaaaa
    14881 gggcggagat gatctccgcc cttttcttat agcttcttgc cggatgcggc gtgaacgcct
    14941 tacccggcct acaaaatcat gcaaaatcaa tatattgcag gagtctcgta ggactaataa
    15001 gcgtagcgca tcaggcacgc tcgcatggtt aacgccatta aatatcaata ttcgccgctt
    15061 tcagggcgtt ctcttcaata aacgcacgac gcggttctac ggcgtcgccc atcagcgtgg
    15121 tgaacaactg gtcagcggca atcgcatcct taacggtaac gcgcagcata cgacggcttt
    15181 ccgggtccat ggtggtttcc cacagctgtt ccgggttcat ctcgcccaga cctttataac
    15241 gctggatgga gaggccacga cgggactctt tcaccagcca gtccagcgcc tgctcgaagc
    15301 tggctaccgg ctgacgacgc tcgccacgtt cgataaacgc atcttcttcc agcaagccac
    15361 gcagtttctc acccagcgtg cagatacgac gatattcgcc accggtgata aactcgtgat
    15421 ccagcggata gtcagtatcc acaccgtggg tacgcacgcg aacaatcggc tcgaacaggt
    15481 tttgttcggc attggtgtgg acatcaaact tccactggct gccgtgctgt tctttgtcgt
    15541 tcagttcgct gaccagcgcg ttcacccagc gggtaacggt ctgctcatca gaaaggtcag
    15601 cttccgtcaa cgtcggctga tagataagct ctttcagcat tgctttcgga taacgacgct
    15661 ccatgcggtt gatcattttc tgcgtcgcgt tgtactcaga taccagtttc tctaacgctt
    15721 cgccagccaa tgccggtgca ctggcgttgg tgtgcagcgt tgcgccgtcc agcgcgatag
    15781 agatctggta ctgatccatc gcttcgtcgt ctttaatgta ctgttcctgc ttgcctttct
    15841 tcactttgta cagcggcggc tgagcgatgt agacgtgacc gcgttcaacg atttccggca
    15901 tctgacgata gaagaaggtc aacagcagcg tacgaatgtg cgagccgtcg acgtccgcat
    15961 cggtcatgat gatgatgctg tgataacgca gtttgtccgg gttgtactcg tcacgaccga
    16021 taccacagcc aagcgcggtg ataagcgtcg ccacttcctg agaagagagc atcttatcga
    16081 agcgcgcttt ctcgacgtta aggattttac ccttcagcgg cagaatcgcc tggttcttgc
    16141 ggttacgccc ctgcttcgca gagccgcccg cggagtcccc ttccaccagg tacagttcgg
    16201 aaagcgccgg atcgcgttcc tggcagtctg ccagtttgcc cggcaggcca gctaagtcga
    16261 gcgcaccttt acggcgggtc atttcacgcg cgcgacgcgc cgcttcacgg gcacgggcag
    16321 catcgataat tttgccaacc acgattttcg catcagttgg gttttccagc aggtattccg
    16381 ccagcagttc gttcatctgc tgttcaaccg ccgatttcac ctcagaagaa accagtttgt
    16441 ctttggtctg ggaggagaat ttcgggtccg gcacttttac ggaaacgacc gcaatcaggc
    16501 cttcacgcgc atcgtcaccg gtggcgctga ctttggcttt tttgctgtag ccttctttgt
    16561 ccatgtaggc attcagggta cgggtcatcg ccgcacggaa gcctgccagg tgagtaccgc
    16621 cgtcacgctg cggaatgttg ttggtaaagc agtagatgtt ttcctggaag ccatcgttcc
    16681 actgcagcgc cacttcaacg ccaataccgt ctttttcggt ggagaagtag aagatatttg
    16741 ggtggatcgg cgttttgttc ttgttcagat attcaacgaa cgctttgatg ccgccttcat
    16801 agtggaagtg gtcttctttg ccgtcacgct tgtcgcgcag acgaatggaa acgccggagt
    16861 tgaggaacga caactcacgc agacgtttcg ccagaatttc atattcgaac tcggtcacat
    16921 tggtgaaggt ttcgaggctg ggccagaaac gcaccatggt gccggttttt tcagtctcgc
    16981 cggtaaccgc cagcggagcc tgcggtacac cgtgttcgta gatctgacgg tgaattttac
    17041 cctcgcgctg aataaccagc tccagttttt gcgacagggc gtttactacc gaaacaccaa
    17101 cgccgtgcag accgccggac actttatagg agttatcgtc aaatttaccg cctgcgtgca
    17161 gaacggtcat gatcacttcc gccgccgata cgccctcttc cgggtgaata ccggtcggaa
    17221 tgccgcgccc gtcatcctgt acagagacag agttatcggc gtgaatggtg acgataattt
    17281 ctttacagtg acccgcgagc gcttcgtcga tagcgttatc taccacctcg aataccatgt
    17341 ggtgcagacc ggtgccgtca tccgtgtcgc cgatatacat acccgggcgc ttacgcaccg
    17401 catccagccc tttcaggact ttgatactgg aggagtcata agaattcgac atcaacgttt
    17461 ctcgctcatt tatacttggg ttaatccgtt attttaccct tttccacggt aaacatcttc
    17521 gaattttcgt ccgacatgtc tataacgtgt tcagcactga tcgcgctgac aaagacctgt
    17581 gattgcgtcg cttttaagcg gctggcaagc aacccgcgac gctcatcatc aagctcagag
    17641 gcaaaatcat ctatcaggta gagacaccgc cgcccgcttt cacgagtgag gaactctcct
    17701 tgcgccagac gtaaggcgca catcaacagc ttaagctgcc cacgcgataa ggtatcttcc
    17761 accggcgcac cgtcggcgcg aatgcgtaag tccgctttat gcgggccatg cgcggtgtag
    17821 gttagctggc gatcgcgttc aaaattacgt tccagcacct cagcgtactc tgtctctttc
    17881 tcccagccgc gctggaaaga gaaagtcaga gaaaactcag ggagaaattg cttacaggta
    17941 tcatccatat cggccgcgat accggcgcta tactccgcgc gccaggtgct gatttgctcc
    18001 gccagcggga tcagttcttt atcccacggg cgtagctgtt cgtaacgagt cacctggcgc
    18061 agcgccgcat tgcgctgctt gagcaatcgc ttgagattgc tccaggcggt gaaaaatcca
    18121 ggttcgttgt gaaagcatcc ccagtcgagg aatgctcttc tgtatttggg gccgccgttg
    18181 agtaaagtaa acccttctgg cgttatcaac tgcatcggca tcaggtgcgc cagttccgcg
    18241 actttatgcc cgtccgtacc gtcaatacgg actttgctgt cgccctgttt gtctttggtt
    18301 aagccaatcg ccgtctcgcg ctcttcgccc tgtaatcgcc cgtggagaac aaatgcctcc
    18361 tgctcatggc gaatgacgcg accaatctgc aaactgcgaa acgcccgacc atggccgagc
    18421 gtatagatgg cttccagcac gctggttttg ccactgccgt tggcacctac cagaaagtta
    18481 aagccgggag ataaagcgag atccgcggtt tcaatattgc ggaaatcgcg gatcaacaag
    18541 cgggtgaggg acattacagt ctcattggca tgacaacata ggcagccgac tgtgatgcgg
    18601 catcttcaat ctgcacgctg gaaaccgaat cggtcagcat catgcggacg ttttcgcatt
    18661 tcagcgcgtt cagaacatcc agcacatagc tgacgttgaa gccgatttcc atctccgcac
    18721 cgctataggt aacgtcgagg atctcttccg cttcttcctg ttccgggttg ttggcagtaa
    18781 ttttcagctg gttttcgctg acatacaggc gcacaccgcg gaatttctcg ttagagagaa
    18841 tcgccgcgcg agcaaacgcc tgcttgagca gatcgcagcc tgcttccaga tgtttgtccg
    18901 ggttcttcgg cagaacgcga cggtaatccg ggaagcgacc atccaccagt ttcgaggtga
    18961 aaataaagtc gccaacgtgg gcacgaatgt tattgctgcc aatctgcaca cgcagcagat
    19021 tgtcgccgcc gtcgagcata cgcatcagtt caatcacgcc tttacgcggt acgatcaccg
    19081 aatggcttgg caaagattga ccaattggca ttgaacagac cgccagacgg tggccgtcgg
    19141 ttgccacggt gcgcagttct tcaccttcgg tttcaaacag cataccgttt aagtaatagc
    19201 gaacatcctg atgcgccatc gaaaactggg ttgcttcaat cagacgcttc atcgtcgcct
    19261 gcggcagggt aaattcgact tcgctctgcc agtcatcgag gttcgggaaa tccgccgctg
    19321 gcagggtaga cagcgaaaaa cggctacgcc cggagcgcac cagcatccgt tcaccttcca
    19381 gctgcacggc aatttccgcg ccttcaggca gaccacggca gatatcaaag aatttgcgcg
    19441 ccggaacggt cgtcgctcct ggctcgtgtg gctgaaccag cgcaacgcgt gccaccattt
    19501 ccatctcgag atcagtaccg gtcagcgaca acgtaccgtc agcaacctgt aacagcagat
    19561 taccgagaat cggtagcgta ggacgaccac ctaacggacc gctcacctgt tgtagcggtt
    19621 ttaataaatg ctcacgttct acggtaaatt tcataggttt acgatgacaa tgttctgatt
    19681 aaatttgaaa aatcttcttt gatatcgtgg ctctcttcac gcaactgctc gatcttacgg
    19741 caggcatgaa gcaccgtcgt gtggtcacgc ccaccaaacg catcgccaat ctctggcaga
    19801 ctgtggttag tcagctcttt cgctagcgcc atcgccatct ggcgcggacg cgccaccgag
    19861 cgggatcgac gcttggaaag gagatccgcg actttgatct tgtagtactc cgccaccgtc
    19921 ttctgaatat tgtcgatggt gaccagtttt tcctgcaatg ccagcaagtc gcgcagcgcc
    19981 tcacgcacga agtcgatggt gatcgcccgt ccggtaaagt tggcgttagc aataacgcgg
    20041 ttcagcgccc cttccagctc acgtacgtta gatcgtagac gcttggcgat aaagaacgcc
    20101 acttcacccg gcaaacgaat gtcattttcg tcggcctttt tcatcaggat cgccacacgg
    20161 gtttccagct ctggcggttc gatcgccaca gtcagtcccc aaccgaagcg ggatttcaaa
    20221 cgatcctcaa cgccgttgat ctctttcgga tagcgatccg aggtgagaat gatctgttga
    20281 ttaccttcca gcagggcgtt gaaggtgtgg aaaaactctt cctgagatcg ttctttatta
    20341 gcaaaaaact gaatatcgtc gatcagcagt gcatctacgg aacggtagta gcgtttaaac
    20401 tcttcgatcg cgttgttttg cagggcttta accatgtcct gaacaaagcg ctcggagtgc
    20461 atataaacca ctttggcatt cggcttgcgc gccataatgc cgttacccac cgcatgcagc
    20521 aggtgagttt tacccagacc cgtgccgcca taaaggaaca acgggttata ggcaccgcca
    20581 gggttatccg ccacctggca agccgccgcg cgcgccagtt ggttagattt accttcaacg
    20641 aagttatcaa acgtgtgttt gacgtttacg ttagaacgat aggtcggttc tgccggggcc
    20701 gggacgttat cccaacctga gcgcgtagaa ggcgcagcac gttgcggctg cgtttgcgcc
    20761 acctgtgcag gggccgcgac gttgctcgtc actgccgctt gtggcgtttg cgtcaccggt
    20821 ttggtgccga cttcaaaacg cagctgtggg gcatccgctc cgcagaaact ggttagcagt
    20881 ccattgatat tattaaggta cttgtcccgt acccaatcga ggacaaaacg gtttggcgcg
    20941 tacagggcca gcgtgttatc gctcagttcc gcctgcaatg ggcgtatcca catactgaat
    21001 tctgtggctg gtaactcatc ctgcaatcgg gcaagacact gctgccaaag cgaaagtgac
    21061 acggcggact ccactcgaac aaaagtcgat aatgactaag gctgaaacat tcatgattgt
    21121 tgacgtacgt cgaaaagacc ctgcttgagg gtgacgcacg aaccgctgtc tgcggttata
    21181 tgcccgatca agatcctgca aaacgatcgg gaccgcggat catagcctaa actgcgcaag
    21241 agatcttctg tttctcacag attttcccta tttatccaca ggactttcca gaactcgcta
    21301 agtgtaaacg atcctgccgc gaggcgggca cgatttacgc cgcatattgg aaaatttaat
    21361 gaccatagac aaaaattggc ttaatcgatc taataaagat ccaggacgat ccttgcgctt
    21421 tacccatcag cccgtataat cctccacccg gcgcgccatg ctggtttcca ctggtgtgag
    21481 gtcgtacatt ttccctgcga aaaggtgcgg aaaagcgcgg taaataagga aagagaattg
    21541 actccggtgt gtacaattat tacaatccgg cctctttaat cacccatggc ttcggtgtcc
    21601 atcgtttcat ttttcggcgg atatccaata aagccattga atttattcaa gtttaggtag
    21661 aaatcgccat gaaacgcact tttcaaccgt ctgtactgaa gcgcaaccgt tctcacggct
    21721 tccgtgctcg tatggctact aaaaatggtc gtcaggttct ggcacgtcgt cgtgctaaag
    21781 gccgcgctcg tctgaccgtt tctaagtaat aaagctaacc cctgagtggt taagctcgca
    21841 tttcccaggg agttacgctt gttaactccc agtcaattca cattcgtctt ccagcagcca
    21901 caacgggctg gcacgccgca aattaccatt ctcggccgcc tgaattcgct ggggcatccc
    21961 cgtatcggtc ttacagtcgc caagaaaaac gttcgacgcg cccatgaacg caatcggatt
    22021 aaacgcctga cgcgtgaaag cttccgtctg cgccaacatg aactcccggc tatggatttc
    22081 gtggtggtgg cgaaaaaagg ggttgccgac ctcgataacc gtgctctctc ggaagcgttg
    22141 gaaaaattat ggcgccgcca ctgtcgcctg gctcgcgggt cctgatagcc ctcattcggg
    22201 tctatcaacg cctgattagt ccgctactcg ggccgcattg tcgtttcact ccaacctgtt
    22261 caagctacgg aattgaggca ttgcgcaggt ttggagtgat aaaaggcagt tggttgacgg
    22321 tgaaacgcgt attaaaatgc caccctttac accctggtgg tgacgatccc gtcccgcccg
    22381 gaccatttga taccagagaa cactaacgat ggattcgcaa cgcaatcttt tagtcatcgc
    22441 tttgctgttc gtgtctttca tgatctggca agcctgggag caggataaaa acccgcaacc
    22501 tcaggcccaa cagaccacgc agacaacgac caccgcagcg ggtagcgccg ccgaccaggg
    22561 cgtaccggcc agtggccagg ggaaactgat ctcggttaag accgacgtgc ttgatctgac
    22621 catcaacacc cgtggtggtg atgttgagca agctctgctg cctgcttacc cgaaagagct
    22681 gaactctacc cagccgttcc agctgttgga aacttcaccg cagtttattt atcaggcaca
    22741 gagcggtctg accggtcgtg atggcccgga taacccggct aacggcccgc gtccgctgta
    22801 taacgttgaa aaagacgctt atgtgctggc tgaaggtcaa aacgaactgc aggtgccgat
    22861 gacgtatacc gacgcggcag gcaacacgtt taccaaaacg tttgtcctga aacgtggtga
    22921 ttacgctgtc aacgtcaact acaacgtgca gaacgctggc gagaaaccgc tggaaatctc
    22981 ctcgtttggt cagttgaagc aatccatcac tctgccaccg catctcgata ccggaagcag
    23041 caacttcgca ctgcacacct tccgtggcgc ggcgtactcc acgcctgacg agaagtatga
    23101 gaaatacaag ttcgatacca ttgccgataa cgaaaacctg aacatctctt cgaaaggtgg
    23161 ttgggtggcg atgctgcaac agtatttcgc gacggcgtgg atcccgcata acgacggtac
    23221 caacaacttc tataccgcta atctgggtaa cggcatcgtc gctatcggct ataaatctca
    23281 gccggtactg gttcagcctg gtcagactgg cgcgatgaac agcaccctgt gggttggccc
    23341 ggaaatccag gacaaaatgg cagctgttgc tccgcacctg gatctgaccg ttgattacgg
    23401 ttggttgtgg ttcatctctc agccgctgtt caaactgctg aaatggatcc atagctttgt
    23461 gggtaactgg ggcttctcca ttatcatcat cacctttatc gttcgtggca tcatgtaccc
    23521 gctgaccaaa gcgcagtaca cctccatggc gaagatgcgt atgctgcagc cgaagattca
    23581 ggcaatgcgt gagcgtctgg gcgatgacaa acagcgtatc agtcaggaaa tgatggcgct
    23641 gtacaaagct gagaaggtta acccgctggg cggctgcttc ccgctgctga tccagatgcc
    23701 aatcttcctg gcgttgtact acatgctgat gggttccgtt gaactgcgtc aggcaccgtt
    23761 tgcactgtgg atccacgacc tgtcggcaca ggacccgtac tacatcctgc cgatcctgat
    23821 gggcgtaacg atgttcttca ttcagaagat gtcgccgacc actgtgaccg acccgatgca
    23881 gcagaagatc atgaccttta tgccggtcat cttcaccgtg ttcttcctgt ggttcccgtc
    23941 aggtctggta ctgtactata tcgtcagcaa cctggtaacc attattcagc agcagctgat
    24001 ttaccgtggt ctggaaaaac gtggcctgca tagccgcgag aagaaaaaat cctgattcgg
    24061 tgagttttcg ctaaaataag ggcggtcagt tgaccgcctt ttttcttttc gtagggcgga
    24121 taagcaccgc gcatccgcca cacaaagcaa caggaacatc atgagcgata atgacactat
    24181 cgtagcccag gccacgcctc cgggacgtgg cggcgttggc atcctgcgca tctccggctt
    24241 caaagcccgt gaagttgccg aaaccgtgct gggtaaactg cctaagccgc gctacgccga
    24301 ttatcttccg tttaaagacg ccgacggcag cgtgctcgat caggggattg cgctatggtt
    24361 ccctggcccg aactcgttca ccggcgaaga tgtgctggaa ctgcaaggtc atggcggtcc
    24421 ggtgatcctc gacctgctgt taaaacgcat tctgaccatt cccggcctgc ggattgctcg
    24481 ccctggtgag ttttccgaac gcgcgtttct taacgataaa cttgacttag cccaggccga
    24541 ggcgattgcc gatcttatcg acgccagttc ggaacaggcg gcccgttcgg cacttaactc
    24601 gctgcaaggc gcattctccg cacgggttaa tcatctggta gaagccctca cccacttgcg
    24661 catttacgtc gaagcggcaa ttgatttccc tgatgaagag atcgatttcc tctccgacgg
    24721 caaaattgaa gcccagctta atgacgtgat ggccgatctc gatgcagtgc gtgctgaagc
    24781 acgtcagggt agtttgttgc gcgaagggat gaaagtagtg attgccggac gtcctaatgc
    24841 cggtaaatcg agcctgttaa acgcgctggc gggccgtgaa gcggcaatcg taaccgatat
    24901 cgccggaacc acgcgtgacg tgctgcgtga gcatatccac attgacggaa tgccgctgca
    24961 tatcatcgac actgccgggc tacgtgaggc cagtgacgaa gtggaacgta ttggtatcga
    25021 gcgcgcgtgg caggaaattg aacaggccga ccgcgtgctg tttatggtcg atggcaccac
    25081 gacagacgcc gttgatccgg cagagatctg gccggaattt atcgcccgtc tgcccgcgaa
    25141 actgccgatc accgtggtac gcaataaagc cgatatcacc ggcgaaacgc tgggaatgag
    25201 tgaagtgaac ggtcacgcgt taattcgtct ctcggcaagg actggtgaag gcgtggacgt
    25261 gctgcgtaac catctcaaac agagcatggg ctttgacacc aatatggaag gcggcttcct
    25321 ggcgcgccgt cgccacttac aggcgctgga acaggcagcg gagcatttgc agcaaggtaa
    25381 agcgcaactg ttgggagcct gggcaggtga actgctggcg gaagagttgc gcctggcaca
    25441 gcagaactta agcgaaatca ccggggaatt tacttcagac gacctgctgg ggcggatttt
    25501 ctccagcttc tgtattggta agtaaccgcg cttacgaagc cgtattctga ctgtcagatg
    25561 cggcttcgct tcattgttag cactcctgtt atgcctcaac ccttttttaa acattaaaat
    25621 tcttacgtaa tttataatct ttaaaaaaag catttaatac tgctccccga acgattgtga
    25681 ttcgattcac atttaaacaa tttcagaata gacaaa
//
LOCUS       DADLTY010000047        25659 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.047, whole genome shotgun sequence.
ACCESSION   DADLTY010000047 DADLTY010000000
VERSION     DADLTY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.047"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="JN334_001614"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="JN334_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HAY7437784.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /locus_tag="JN334_001615"
     CDS             1794..2540
                     /locus_tag="JN334_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HAY7437785.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="JN334_001616"
     CDS             2630..3391
                     /locus_tag="JN334_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAY7437786.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="JN334_001617"
     CDS             complement(3442..4326)
                     /locus_tag="JN334_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HAY7437787.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="JN334_001618"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="JN334_001618"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY7437788.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="JN334_001619"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="JN334_001619"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAY7437789.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="JN334_001620"
     CDS             6202..6474
                     /locus_tag="JN334_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HAY7437790.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="JN334_001621"
     CDS             6844..7920
                     /locus_tag="JN334_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7437791.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="JN334_001622"
     CDS             7947..9326
                     /locus_tag="JN334_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7437792.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="JN334_001623"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="JN334_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="JN334_001624"
     CDS             10409..11245
                     /locus_tag="JN334_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAY7437793.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="JN334_001625"
     CDS             11250..12197
                     /locus_tag="JN334_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HAY7437794.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="JN334_001626"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="JN334_001626"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HAY7437795.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDANATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="JN334_001627"
     CDS             13324..14082
                     /locus_tag="JN334_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7437796.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="JN334_001628"
     CDS             14200..15558
                     /locus_tag="JN334_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7437797.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="JN334_001629"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="JN334_001629"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HAY7437798.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="JN334_001630"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="JN334_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAY7437799.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="JN334_001631"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="JN334_001631"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAY7437800.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="JN334_001632"
     CDS             19503..20054
                     /locus_tag="JN334_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAY7437801.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="JN334_001633"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="JN334_001633"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAY7437802.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /locus_tag="JN334_001634"
     CDS             21219..23180
                     /locus_tag="JN334_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAY7437803.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="JN334_001635"
     CDS             23307..24347
                     /locus_tag="JN334_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY7437804.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..>25659)
                     /gene="gdhA"
                     /locus_tag="JN334_001636"
     CDS             complement(24464..>25659)
                     /gene="gdhA"
                     /locus_tag="JN334_001636"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAY7437805.1"
                     /translation="LERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGG
                     MRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMT
                     ELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEA
                     TGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTV
                     VDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNE
                     LDVDAAHQLIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEM
                     AQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAML
                     AQGVI"
BASE COUNT         6517 a         5971 c         6624 g         6547 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 gaatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcact atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 caaacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcggca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc ggcgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaag ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatttgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgccg ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
    25261 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
    25321 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgtctggcaa aaacgcatca
    25381 ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca ccgcccatcg
    25441 gcagggtagt cagggcattt ttgaaggttt gttcaaagcc gaggaatttg agaatggaaa
    25501 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
    25561 gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat accacgcgaa
    25621 actggatcac gcgctccggt tcaaccagac gctccagta
//
LOCUS       DADLTY010000048        24993 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.048, whole genome shotgun sequence.
ACCESSION   DADLTY010000048 DADLTY010000000
VERSION     DADLTY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.048"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(40..447)
                     /locus_tag="JN334_001637"
     CDS             complement(40..447)
                     /locus_tag="JN334_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HAY7437806.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(444..1007)
                     /locus_tag="JN334_001638"
     CDS             complement(444..1007)
                     /locus_tag="JN334_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY7437807.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(998..1468)
                     /locus_tag="JN334_001639"
                     /pseudo
     CDS             complement(998..1468)
                     /locus_tag="JN334_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(1652..2446)
                     /gene="thyA"
                     /locus_tag="JN334_001640"
     CDS             complement(1652..2446)
                     /gene="thyA"
                     /locus_tag="JN334_001640"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAY7437808.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(2453..3328)
                     /gene="lgt"
                     /locus_tag="JN334_001641"
     CDS             complement(2453..3328)
                     /gene="lgt"
                     /locus_tag="JN334_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAY7437809.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(3479..5725)
                     /gene="ptsP"
                     /locus_tag="JN334_001642"
     CDS             complement(3479..5725)
                     /gene="ptsP"
                     /locus_tag="JN334_001642"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7437810.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(5738..6268)
                     /gene="rppH"
                     /locus_tag="JN334_001643"
     CDS             complement(5738..6268)
                     /gene="rppH"
                     /locus_tag="JN334_001643"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAY7437811.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            6953..7642
                     /gene="mutH"
                     /locus_tag="JN334_001644"
     CDS             6953..7642
                     /gene="mutH"
                     /locus_tag="JN334_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAY7437812.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            7711..8424
                     /locus_tag="JN334_001645"
     CDS             7711..8424
                     /locus_tag="JN334_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY7437813.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            8562..8780
                     /gene="ygdR"
                     /locus_tag="JN334_001646"
     CDS             8562..8780
                     /gene="ygdR"
                     /locus_tag="JN334_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HAY7437814.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            8888..9928
                     /locus_tag="JN334_001647"
     CDS             8888..9928
                     /locus_tag="JN334_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HAY7437815.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(9961..11151)
                     /gene="lplT"
                     /locus_tag="JN334_001648"
     CDS             complement(9961..11151)
                     /gene="lplT"
                     /locus_tag="JN334_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HAY7437816.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(11144..13303)
                     /gene="aas"
                     /locus_tag="JN334_001649"
     CDS             complement(11144..13303)
                     /gene="aas"
                     /locus_tag="JN334_001649"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HAY7437817.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            13889..14920
                     /gene="galR"
                     /locus_tag="JN334_001650"
     CDS             13889..14920
                     /gene="galR"
                     /locus_tag="JN334_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HAY7437818.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(14927..16189)
                     /gene="lysA"
                     /locus_tag="JN334_001651"
     CDS             complement(14927..16189)
                     /gene="lysA"
                     /locus_tag="JN334_001651"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAY7437819.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            16311..17246
                     /gene="lysR"
                     /locus_tag="JN334_001652"
     CDS             16311..17246
                     /gene="lysR"
                     /locus_tag="JN334_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HAY7437820.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(17233..17925)
                     /gene="ygeA"
                     /locus_tag="JN334_001653"
     CDS             complement(17233..17925)
                     /gene="ygeA"
                     /locus_tag="JN334_001653"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY7437821.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(18054..19472)
                     /locus_tag="JN334_001654"
     CDS             complement(18054..19472)
                     /locus_tag="JN334_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7437822.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(19787..20548)
                     /gene="kduD"
                     /locus_tag="JN334_001655"
     CDS             complement(19787..20548)
                     /gene="kduD"
                     /locus_tag="JN334_001655"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HAY7437823.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(20578..21414)
                     /gene="kduI"
                     /locus_tag="JN334_001656"
     CDS             complement(20578..21414)
                     /gene="kduI"
                     /locus_tag="JN334_001656"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAY7437824.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(21701..22882)
                     /locus_tag="JN334_001657"
     CDS             complement(21701..22882)
                     /locus_tag="JN334_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAY7437825.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            23248..24366
                     /locus_tag="JN334_001658"
     CDS             23248..24366
                     /locus_tag="JN334_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY7437826.1"
                     /translation="MVLFITALVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYY
                     GKKIGNLITTLYFIAFFVVVLIYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILN
                     LIFLMGRHATIRVMGFLVFPLIAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWIS
                     IPVMVFAFSHTPIISTFAIDRREKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLS
                     IPSSYIEAAKEEGVTILSALSMLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGAT
                     EVVKTTLQQVGVKKSRAFNHALSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILF
                     IMPTLSTYLIPALKPWRSIGNLITLIVGILCVSVMFFS"
BASE COUNT         6439 a         6622 c         5926 g         6006 t
ORIGIN      
        1 caaccattac ctccggcagg ctaaagcctt gttgattctt cagggaagct gacataatgc
       61 cccctctttc agcggacaaa aatcgctcca gccgcgtggc gaaaaaacaa tttttccatc
      121 aatgacttcg cctgttcgcc acaacgaaac gccttcataa ccggcaatca ataaggcttc
      181 attatctgcc agtaaacgca aacaaacctg ggcatcggtt ccagcgtact gcgagcactg
      241 aactgctggc tgcgtctgcc aggagtgcat ttttccccac gccagcgccg actgaacgat
      301 ggcctggcgg cgcaatgact gactttccat gctcacgcga gaggcaaaac tgcgatcctg
      361 ctgactcatt ccttgcaata gcaagctacc caaaaccagc agcatcagga ccagagccag
      421 tgacgaaaca cctttttcgc ggttcacagg ttgaatcctg tcacgctata gctggcatcc
      481 accacggttt gcggttcaga cttactggca gcacgcatat taaccgtcaa caccggcgag
      541 aagccgctga catcctgacg tacgacctga aaagtgtcga taatgatggc atccggatta
      601 gtgactttat cccagccctt accttcacag gatgtcgcac cgcgtagcgt ttccagcaca
      661 tgctccttca gacgaaatcc aatctggtcg gactctttta ccggttcgcg atcccagata
      721 ccgttactgt tcgcatccca ctgcacaatg atacagtcac cctgtccgac aatttccagc
      781 ccttcgccgg tacagctgcc atgacaataa cccgccctct ggagatgctt cgcgacggta
      841 aatacccgca gccagatttc atcttccagt gccagcttac gagtgctcgt taaactttca
      901 cgctgtaacg caggcagaaa gcgtgcagcc cccagcaaca atacgctact gatcgccata
      961 gcaatcaaca cttccagcag agaaaaacct tgctctttta caggcatcct tctgtttctc
     1021 cttgctgata aagccggagt cttccccacg gcgaaaccac cagccaccac tcgcccgttg
     1081 agtttttgaa gcgaatatgc ccggcccatg cggtattgcg caggccaaag aaagcaagcg
     1141 aaggtgtcag gtcgctcatt tcgacttcgg gccagcgtgg cacaaagacc aatggtgaac
     1201 tgccatgaca ggtattggcc ccagcagcgg aactcacaag gcaccataac gtcccctccc
     1261 tgataacgct gatactgtgg tcgcggttat gccagttggc atcttcacgt aaatagagca
     1321 aatagtcccg cgcctagctg gcggtttgcc aaagccgttg cgactgctgc cagtattgcc
     1381 agccatagag tccacttgcg cttagcatga ccaaaatcag catcgcgacc agcgtttcaa
     1441 tcagcgtata accacgttgt gttttcatgc cggcagtatg gagcgaggag aaaaaaagac
     1501 gagggccagt ttctatttct tcggcgcatc ttccggacta tttacgccgt tgcaggacgt
     1561 tgcaaaattt cgggaaggcg tctcgaagaa tttaacggag ggtaaaaaaa ccgacgcaca
     1621 ctggcgtcgg ctctggcagg atgtttcgta attagatagc caccggcgct ttaacgcccg
     1681 gatgtggatc gtagccttca atctcaaagt cttcgaaacg gtagtcgaag atggattcgg
     1741 gtttacgttt gataatcaac ttcggcagcg gacgcggttc gcggcttaat tgcagatgag
     1801 tttgatccat atggttgctg tacaggtgcg tatcgccacc ggtccagaca aaatcaccca
     1861 cttccagatc gcactgctgc gccatcatat gcaccagtaa cgcgtagctg gcaatgttga
     1921 acggcaggcc gaggaagacg tcacaggagc gctgataaag ctggcaagag agtttgccgt
     1981 ctgccacata gaactggaag aatgcatggc acggtgccag cgccatttta tccagttcgc
     2041 ctacgttcca cgctgaaaca ataatgcggc gcgaatccgg gtcgtttttc agctggttca
     2101 gtaccgtagt aatctggtca atatgacgac catctggcgt tggccaggcg cgccactgtt
     2161 taccatacac tggcccgagg tcgccatttt catcggccca ttcgtcccag atggtgacat
     2221 tgttttcgtg tagataagca atgttagtat cgccctgcag gaaccacagc agttcgtgga
     2281 tgatggaacg caggtggcaa cgtttggttg tcaccagcgg gaatccatcc tgcaggttaa
     2341 aacgcatctg atgaccaaaa atggaaagcg ttccggttcc ggtacggtcg tttttctgtg
     2401 tgccttcgtc gagcactttt tgcatcagtt ctaaatactg tttcatggtt cctcaggaaa
     2461 cgtgttgctg tgggctgcga cgatatgccc agaccatcat gatcacaccc gcgacaatca
     2521 tcgggatgga aagaatttgc cccatgctga tgtactgcac ccaggcaccg gtaaactgcg
     2581 cgtcgggctg gcggaaaaac tcaacaatga tgcgaaacgc gccgtagcca atcaggaaca
     2641 agcctgagac agctcccatt gggcgcggtt tacgaatata caggttgagg ataataaaca
     2701 gcaccacacc ttccagcagc agttcgtaaa gctgtgatgg gtggcgcggc agcacaccgt
     2761 aagtgtcgaa aatggattgc cactgcgggt tggtttgcag cagcaaaata tcttctgtac
     2821 gggagcctgg gaacagcatg gcaaacggga agttcgggtc aacgcggccc cacaattcac
     2881 cgttaataaa gttgcccaga cgcccggcac caagaccaaa cggaatgagt ggtgcgataa
     2941 aatcagagac ctggaagaag gaacgtttag tacggcgggc gaagataatc atcaccacga
     3001 taacgccaat caggccgccg tggaaagaca tgccgccgtc ccagacacgg aacagataca
     3061 gcggatcggc cataaactgc gggaaattgt agaacagaac ataaccaata cgtcccccga
     3121 ggaagacgcc gaggaagccc gcatagagta agttttcaac ttcatttttg gtccagccac
     3181 tgcccggacg attcgcccga cgtgttgcca gccacattgc aaaaatgaac cccaccagat
     3241 acatcaggcc gtaccagtga agcgccacgg gtcctattga gaaaatgacc ggatcaaact
     3301 ccggaaaatg cagatagcta ctggtcatct gtcaccacaa gttcttgtta tttcgctgaa
     3361 agagaacagc gattgaaatg cgcgccgcag gtttcaggcg ctccaaaggt gcgaataata
     3421 gcacaagggg acctggctgg ttgccggata ccgttaaaag atatgtatat gatccgcgct
     3481 ataaccctcc gcgaatcagc ccgcccatgc cgcgacgctc cataaaggct gcaacctgat
     3541 gacgaacttc ggtcgccagt tgcgcttcca gactacgctg cgcaagattt tctgcttcgg
     3601 caaaatcaat gcgccgcagc aggtatttta cccgcgctac agaacgtccg ttcatagaca
     3661 gatggcgata cccaagccca atgaggattg ccacgcacat gggatcgccc gccatttcac
     3721 cgcacaaacg aagatcgata ccatgtattt ccgcttcccg ggcgatcatc gccagagctc
     3781 gtaacattgc aggatgaaga ctgtcgtaaa tgttcgccac ccgggtattg ttgcgatcaa
     3841 cggccagaat gtattgagtc agatcgttgg tgccaacaga gatgaaatcg accctctttg
     3901 ccagatgcgg cagcataaat accattgacg gcacttccag catgatgccg atacgtggtt
     3961 tgggaatttc gtaaccgatc atctcctcga cttcacgtcc ggcacgttca atcaggcggc
     4021 gtgcttcgtc aacttcatcg aggctggtga ccatcggcaa cagaatattc aggttgcccg
     4081 tagcggcatt agcacgcagc atcgcccgca cctggatcaa gaagatctcc ggctgatcga
     4141 gcgtaatgcg aatcccacgc caacccaggc atggattctc ttcgctgatc ggcatgtaag
     4201 gcagctgctt atctgctccg acatccagcg tacgcaaggt gacgggttta tcattgaaca
     4261 tttgcagcat cccctgatac tgcgccacct gttcttcttc cgacgggaaa ccactttgca
     4321 gcatgaatgg gatttcagtg cgataaagac cgatgccatc aatacagctg cccagttttt
     4381 cttcatgttc cgggcttaaa ccagcattga gcatgacttt tatacgctca ccgcttttta
     4441 actgcgcggg taaattgacg tcatcttccg ccagacggct aagctcaatc tcttcactaa
     4501 ttagccgctg atattcttgc agcagtaccg gctccggatc gaccagcaat tcaccgcgat
     4561 agccatcaac gatcagcgtc ctacgatgca gcaccgaagg ctgaatatcc gcgcccatca
     4621 cggtagggat ccccagcgca cgtaccatga tcgcagcatg ggagttggct gcgccatctc
     4681 gcacgacaac accgactaag cgatcctggg gcagctcagc aagagttgtc gctgacagtt
     4741 catctgccac cagaatgaaa cgttccggcc aggcgttcgg cccttgatta gcgtcatcaa
     4801 gatgaaacag caatcgctga cccagcgcac gtaaatcacc agcccgctct ttgagatagt
     4861 tatcgcttag cgcggcaaac tgttcggcaa atttttcaat gaccgttttt accgcccact
     4921 ctgccaccga gcctttatca acctcggcaa acaattcgcg acgcagccgg gtgtccgaaa
     4981 gcaggtgcga gtaaagatcg aaaatagccg ccgtttcttt ttgtgctccg gcggcaaagc
     5041 gtttgctgta gcggcgaaac tcgtttgccg cttcttccag cgccccggtc agtcgttcgc
     5101 gttccagagc cggatccagc gttgatgcct gatacacctg ttccattaaa ggtaacgtgg
     5161 catcctgcca gccttcggca atcgccacac caggtgctgc cggtaatgcg cggattcgcg
     5221 tctggcgata ttgcccaaac agggcagtca actgcgactg agaaagaata gctgccatct
     5281 gggtggcaag cgtcaccagg aaggattctt cactttcgtc atactggcgc aactctcgtt
     5341 gctgtaccac cagtacacca agcaactggc gacgttgaat aattggtacg cctaaaaacg
     5401 cgcggaaacg ttcttctttt acggagggga tgtatttgaa gctggggtgc ttttgcgcat
     5461 ctgcaaggtt tatcggttcc gccagcctgc caaccaggcc gacgatccct tcatcaaacg
     5521 cgagcgttac agtgcgaccg cgtggttttt tcagcccccg ggtcgccatc aggtagtaac
     5581 aacgtcgatc atgatcggcc aggtagaccg aacagacctc ggtatccatc gcaagacaga
     5641 tgtcggtaac cagaatattt aacgcctcat tcaggcgtgg tgcgctggct accttttcga
     5701 ctatttcgcg caggcgagtg agcataattg gcgtgactta acctctttta cgtcgataag
     5761 cagatgcgtt ttgtggtttt ggcgtatttt cctgcagtga catcaccaca ctcgcgaact
     5821 ctttcattac cctacggtag acatcacgtt taaatgacac cacctgtctg accggatacc
     5881 agtaacttac ccatcgccag ccgtcaaact ctggtgtact gctggtttgc atattgattt
     5941 ctgcatcgcc gcttaccagc tgcaagagaa accatttttg tttttggccg atacaaaccg
     6001 gcttcgtgtc ccaacgcacc aaacgtttcg gtaatttgta gcgcaaccag ttacgcgttg
     6061 aagcaaggat tcgaacgtct ttgcggctta atcctacttc ttcaaacaat tcacggtaca
     6121 tcgcctgctc tgcggattct ccggggttga ttccgccttg cggaaattgc caggagtgct
     6181 gaccaaatcg ccgggcccac attacctgcc cctggcgatt acaaatcacg atacctacgt
     6241 ttgggcggta gccatcgtca tcaatcaccg gactacctca aaataaagct ttatatacga
     6301 atgattgttt catactccag gaagacggta aaccactctc tgcagggcat tacacactaa
     6361 taacaattga ataactcaca gttatgtgca gagttataaa cagaggaagg ggtggatagc
     6421 cgtttttatt caccttttct gtggatagag ttgtgaagaa ctacggaatt actacgggaa
     6481 aacccggagc attctgaata agcccgatat agacatttta aaatattcat tattagtcaa
     6541 tgcattacat gtaatttgag taatgaaaaa gagtaattcg tgacccaggt cacacctctc
     6601 atttacgggt tggcgaaaga tcaaccaatg ccgtgtttat ccacagaatg tgccactaag
     6661 ttaagcactg aaccactaaa aactggagtt tcgtcgcacg tcaaggctgt aaatgaaaac
     6721 agtagtggag gtttttcaca gttatcccag ctttctgtgg ataacatggt gtaagatcct
     6781 gtttattttc agtgaccaga tttggaaaac ctattgcagt gttgcgcaac tcgattaccg
     6841 gcagcctaaa aagcaatata aatcagagaa ttgaacaaca catgtggaaa aagttacact
     6901 gcgaatattc ggcacataat tgctgtttgt tttttaacca aggtatcatg acatgtccca
     6961 acctcgccca ctgctctctc ctcccgaaac tgaagaacag ttgttagcgc aagcacagca
     7021 actttctggt tatacattgg gagaactggc ggcacttgac gggctggtta cgccagagaa
     7081 tttaaaacgc gataagggct ggattggcgt gttactggag atctggctag gtgccagcgc
     7141 agggagtaaa cctgagcaag attttgctgc tctgggcgtg gaacttaaaa ctatccctgt
     7201 ggatagtctt ggtcgtccgc tggaaacaac attcgtttgt gttgcccggt taacgggtaa
     7261 tagcggggtg acctgggaaa ccagccatgt gcgccacaag ctcaaacgcg tactgtggat
     7321 accggttgaa ggcgagcgca gcatcccgct ggcgcagcgt cgcgtaggat caccgttgct
     7381 gtggagcccg aatgaagagg aagaccgaca gctacgcgaa gactgggaag aattaatgga
     7441 tatgattgtt ctcggtcagg ttgagcggat caccgctcgt cacggggaat atttacagat
     7501 aagaccgaaa gcagcgaatg cgaaagcgct taccgaagcc attggtgccc ggggcgaacg
     7561 gattctgacg ctgccacgcg gcttttattt gaagaagaat ttcaccagtg cactactggc
     7621 ccgtcatttt ctgatccagt agccatcgct ttgacctgcc gctttccggg catataatta
     7681 ccgcttcatt tttttggcag ggctttttag atgttatttg catggataac cgatcctaac
     7741 gcttggcttg cgctcggtac gctgacgctg ctggagatcg ttcttgggat cgacaatatt
     7801 attttccttt ctctggtggt ggcaaagctt cccacagcac aacgtgctca tgcgcgccgt
     7861 ctggggttgg cgggagccat ggttatgcgt ctggcgctgc tggcatcaat ctcttgggtt
     7921 acgcgcctga caaatccgct ttttacaata ttcagtcagg aaatttctgc ccgtgatttg
     7981 attctgcttc tgggtggctt gttccttatc tggaaagcca gcaaggaaat ccacgaatcc
     8041 attgaaggtg aagaagaagg gctgaaaaca cgcgtttcat cattcctcgg cgctatcgtg
     8101 cagattatgc tgctggatat tatcttcagc ctcgactcgg tgattaccgc tgtgggtctg
     8161 tcagatcacc tgtttattat gatggcagcc gtggtgattg ccgtaggcgt gatgatgttc
     8221 gccgcgcgct cgattggtga ttttgtcgaa cgccatcctt cggtaaaaat gctggcgctc
     8281 tctttcctga ttctggtggg ctttaccctg attctggaaa gtttcgacat ccacgtaccg
     8341 aaaggttaca tctacttcgc gatgttcttc tctattgcgg ttgaaagcct caacttgatt
     8401 cgcaacaaaa agaatccgct ctgatactcc gtacgctctc ctgcgggagg gcgtactcac
     8461 tccttttacc ctcacagttt aagattttcc tgctttcaag atatataacg tcggtttata
     8521 aacagactat tatcataggt aggccaaatg aggataaccg gatgaaaaaa tgggcagtaa
     8581 taatttctgc agtcggactg gcgtttgctg tttccgggtg ttccagtgat tacgtcatgg
     8641 cgaccaaaga tggccgtatg attttgaccg atggaaaacc tgaaattgat gatgataccg
     8701 ggctggtgag ttatcacgat cagcaaggta acgcgatgca aattaaccgt gatgatgttt
     8761 cgcaaattat tgaacgttaa caaataaggt cagcatccgg ctggccttaa gatttttctc
     8821 tttccctttt cccttccctc tgccattttt atattcctta tgtcgtgatt ataaaaagga
     8881 aacggctatg caatatcacc gtatacccca cagttcgctg gaagtcagca cgctggggct
     8941 tggcacgatg acgtttggtg aacagaacag cgaagccgac gcccacgcac aactcgacta
     9001 tgccgtcgct cagggcatta accttatcga cgttgccgaa atgtacccag tacctccgcg
     9061 ccccgaaacg caagggttaa ccgaaaccta cgtcggcaac tggctggcga aacatggcag
     9121 ccgcgaaaag ttaattatcg cctccaaagt gagcggaccg tcgcgcaata atgacaaggg
     9181 catccgcccg gatcaggcgc tggatcggaa gaatatccgc gaagcgctgc atgacagcct
     9241 caagcgccta cagactgatt acctcgatct ttatcaggtg cactggccgc agcgcccgac
     9301 caactgcttc ggcaaactcg gttatagctg gacggattct gcgcctgcgg tttcgctgct
     9361 ggatacgctg gacgcactgg cagagtacca acgcgcggga aaaattcgtt atatcggcgt
     9421 gtcgaacgaa actgcatttg gcgtaatgcg ctacctgcat ctggcggaca aacacgatct
     9481 gccgcgtatt gtcaccattc agaaccctta cagtctgtta aaccgcagtt ttgaagtagg
     9541 tctggcagaa gtcagccagt atgaaggggt cgaactgctg gcctattcgt gcctgggttt
     9601 cggcacgctg accgggaaat atctcaatgg tgcaaaaccc gctggcgcac gtaatacgct
     9661 ctttagtcgg ttcacccgct atagcggtga gcaaacgcaa aaagccgtcg cggcgtatgt
     9721 tgatatcgcc agacgtcatg gccttgatcc tgctcagatg gcgctcgcgt ttgtacgccg
     9781 tcaaccgttt gttgccagca ctctgctggg cgcaaccacg atggatcagc tgaaaactaa
     9841 catcgaaagt ttgcatctgg agttaagcga agacgtatta gctgaaattg aagcggtaca
     9901 tcaggtttat acttatcctg caccataaag attaacgccg gttaaaaccg gcgttaaata
     9961 ttaatgacgg cgctgccaga tccacagcgc cgttattgcc agcgcaaata gcgcaccgaa
    10021 gccaatgcca atgggcacga ccgggatgcc taccattacc gccagcgagt aaatgcccag
    10081 catcaacaac atggcgctgt tttcgccaag gttttgtact gcaatcgcat tccccgcccc
    10141 gacgcttttt ttaccccgct cctgtagcaa cgcattgagc ggaacgacaa aaaagccccc
    10201 cagcacgcca atcagcatca acaaggcata ggctggcagc agctcgtgtt gcagggagaa
    10261 aatcggtacc accacgccaa tcaaaatccc ggctggcata cagcgtgaca cggtttccag
    10321 cgtaaccagc ttcgctgccg cacctgcgcc aaccacgata ccaatcgcta ccatcgcgtt
    10381 gagataggtc ggcgtagcgt tatcggtaat gcccagcgcc accggtaccc acagcaccaa
    10441 caggaaacgc agcgtgacac ccgctcccca gaataaactg gtgcccacca gcgaaaaacg
    10501 cgtttcacca ttgcaccata gcgaggtgca ggcattcagg aaactgcggg tcatgttgat
    10561 gagattccag gactgccccg gacgcgccgc cagtttggga atgtagatat tggcaacgac
    10621 cgcaccaccg taggccagtg cgcatgcggc cagagcgacc aggacatgcc agtcagccag
    10681 cacaccaccg gctacggaac cgagtaaaat cgccgctatg gcagaagctt ccattaaacc
    10741 gttagctttt actaacttac tacccgtggt taattcgccg agaataccgt atttcgccgg
    10801 tgaataggct gcagcaccaa cacccaccag cgtatagccg agaaacggat tgataccaaa
    10861 gcagatactg gctgcgccca gcagcttcag gccgttggca aacatcatca cccggccttt
    10921 ggcgaagcta tccgccacct gcccgacaaa cggcgccaaa agaatgtaag cacctacaaa
    10981 caccatttgc aggatgggct ggctccactc cggatagaac tgcgctttca gtaacgccag
    11041 agtggcaaac agtagcgcgt tatcgccaaa cgcagagaga aactgcgcca cgataaccgc
    11101 tttcatcccc ttcgaccaca acgaagtgtt agtgtgcact gactcactca tcgtgttgtt
    11161 ccgcttcgtc tacccagctt ttcaacgtga caaagtcagg tttgccgctg ccaagtaatg
    11221 gcatctgttt cagatagcga atatcgcgcg gtacagcaag ctccggcacg ccgtgctcgc
    11281 gggcatactg ttgcaactta tcgcgcgtca gttcgttatc tgtggtgaaa agcaccagtg
    11341 cctcgccttt gctggcatcg ctcttaatcg cagtggcatg gactttatct ggcgaaacac
    11401 caagtgccag ttgttccacc atttccagcg acaccatttc gcctgcaatt ttggcaaagc
    11461 gttttgcgcg gccctgaatc tgcacaaagc cctgctcgtc aaaacgcaca atatcgccag
    11521 tgtcatacca gccgcgctcc atttcgccgc gaacattctc ggcggtgggc acttccagta
    11581 cacctggctt ctccacccgc agatagccgt tcattatgtt cggccctttc agttgcaggc
    11641 gtccgccctc ttcgatacca gggaccgaca acaggcgtgc atccattcct ggtagaatac
    11701 gccctaccgt accgggtttc gccgccatcg gtacgttgat agaaacgaca ggcgcgcatt
    11761 cggtcacacc gtagccttca aggatgcgca ggccaaattt atcctgccaa agctgtttgg
    11821 tactttcttg tagtttttct gcgcctgcca ccacatagcg tagacgatag aagtcatacg
    11881 ggttggcgaa acgcgcgtag tgaccgagga aagtcgaggt accgaacaac acggtgcaac
    11941 tgcggtcata caccaagtcc ggcacaatgc ggtaatgcag cgggcttgga taaaggaaca
    12001 cttctgcacc tgtaagcagt ggcgtaaaca ggcctaccgt cagcccaaag gagtgaaaca
    12061 gcggtaatgc cgacataaag cgatcgttgg tggtgaagtc ggcaatcgtt ttaatctgct
    12121 cgacgttcgc cagaatgctt ttatggctat ggacgacgcc ttttggatgg ccttcagaac
    12181 cggaggtaaa gaggatcagc gcctcttctt ccggctgctg ttttacctgc gccaggcgcg
    12241 gcatcagcaa atgagcgaag atccatactt tgtcggcagt ggtgacatct gcttttaaat
    12301 cttccagata gacccagcgc acctgagtaa gttgctccgg cagatgccag agtttgcctt
    12361 tatcgagaaa ctggcgggaa gtgaagatgg ttttgatttc agccgccgta atagcactgg
    12421 tcagcccttt taccccggca gtgtagttca tcattgcggg aatgcggcga cgcgcgatgg
    12481 ccccaaaaat cactgccgca ctgatgcctg cattgggcag cattaagccg atgcgttcgc
    12541 cttcaacact gtatttttca aggatgcgtc caacaaacag cgttttcgta agcaatttgc
    12601 gataggagtc tggggtaaag ttgacgtctt cgacacattt cttcccggct ccgaagcggt
    12661 acattgcact cagtaaagat tcgtacagag tttcacgcgg gcgcaccgcc atacgcgctt
    12721 ccatcattat ttgatgcagc atttcgccag cgattttgcg acggtcacgg gcacgcggcg
    12781 catccggcat cgccacctgc gttggaggca aaatatgcag agtaatttgc gggaacaagc
    12841 gacgtttaac cagacctttc aggcggctga agtgcgtaag ttccgcccct tcaatacgca
    12901 caggaataac cgttgcacca gacttcgccg cgacaaaacc cgcgccatcg tagattttca
    12961 tcagcgagcc tgtagtggtg atgcgtcctt cagggaaaat caccactggt cggccctgtt
    13021 ccaccagacg taccagatgt ttaatagcca taggttgcgt cgggtcgaga ggaacaaagt
    13081 cgataaatga tttcagccaa cgcatatacc actgttggct tattgaggtg taaacggcaa
    13141 acactggacg cacaggtaaa aacagtccaa gcaaaatgcc atcaataaaa gagacgtgat
    13201 taggcgtaat tagtacgcgc tcgcccttca gtgcctgggt gtcacccgta acgcgaacgc
    13261 gatacaaaac acggcacaaa tttcgaaaaa agctaaaaag cattccaact ccctttgctc
    13321 tgattcagta aaagcgaatg gagggagatt acacgagata aagaacgcga gcgacagtaa
    13381 attaggtgcg aaaaaaaacc tgcgcatccg cgcaggttgg tgcaagagac agggtacgaa
    13441 gagcgtaccg aataatctca ccaatcaata cctctgggat cttgattgtg gtctgcacga
    13501 cgactcttcg ccagcgagaa aacgcaaagg aatgaaggga aatgcaacga ggtgtgtaaa
    13561 ttgtcggtta ctgttacaga ttgatgaccg gcaaaaaaaa acctgcgcat ctgcgcaggc
    13621 tggtgtaatt catgtgctca acacgaagtt gacttcacct atcaatacct ctgggatcac
    13681 cactttagca acctgaagcc aaacgccacc agcggtcaat cgcaacagcg tttcgcaaag
    13741 tgtaaccaaa ggtttgcaat ctcttttttt gtcttactta tttgcgtttg gctcacattc
    13801 ccacgatgaa aacacgccac cccttgaacc aacgggcgtt ttccgtaaca ctgaaacaat
    13861 gtaagcgttt acccactaag gtattttcat ggcgaccata aaggatgtag cccgactggc
    13921 aggcgtttca gtcgccaccg tttcccgcgt cattaataat tcacccaaag ccagcgaagc
    13981 ttcccggctg gctgtgcata gtgcaatgga gtctcttagc tatcacccga acgccaacgc
    14041 ccgtgcgctg gcgcagcaga ccactgaaac ggtcggtctg atcgttggtg atgtttccga
    14101 tccgtttttc ggtgcaatgg tgaaagcggt cgaacaggtg gcttatcaca ccggtaattt
    14161 cttattgatt ggcaacggtt accacaacga acaaaaagag cgtcaggcta ttgagcaact
    14221 gatccgccat cgctgtgctg cgttggtcgt ccatgccaaa atgatcccag atgccgattt
    14281 agcctcatta atgaaacaaa tgcccggtat ggtgctgatc aaccgtatcc tgcctggctt
    14341 tgaaaaccgt tgtattgctc ttgacgatcg ttacggtgcc tggctggcaa cgcgtcattt
    14401 aattcagcaa ggtcataccc gcattggtta tctgtgctct aaccactcta tttctgacgc
    14461 cgaagatcgt ctgcaagggt attacgatgc ccttgctgaa agtggtattc cggccaatga
    14521 tcggctggtg acatttggcg aaccagacga aagcggcggc gaacaggcaa tgaccgagct
    14581 tttgggacga ggaagaaatt tcactgcggt agcctgttat aacgattcaa tggcggcggg
    14641 cgcgatgggc gttctcaatg ataatggtat tgatgtaccg ggtgagattt cgttaattgg
    14701 ctttgatgat gtgctggtgt cacgctatgt gcgtccgcgc ctgaccaccg tgcgttaccc
    14761 aatcgtaacg atggcgaccc aggctgccga actggctttg gcactggcgg ataatcgccc
    14821 tctcccggaa attactaatg tatttagtcc gacgctggta cgtcgtcatt cagtgtcaac
    14881 tccgtcgctg gaggcaagtc atcatgcaac cagcgactaa ccgcagttaa agcaattcca
    14941 gcgccagtaa ttcttcgatg gtctggcgac ggcgaatcaa ccgcgcctga ccattatcaa
    15001 acagaacttc tggtaacagc ggacggctat tgtagttgga tgacattgat gcgccatatg
    15061 cccctgtatc atgcagtacc agataatcac ctgccttcac ttccggcaag gcgcgggttt
    15121 caacatttcc cccttcctgc tgggtaaaga catcgcccga ttcacataac ggcccggcga
    15181 cgacggtttc caccgttggc gcgtgttcca gagaacgacc atcagctgcc agggcactga
    15241 tatggtggta actaccgtac attgccgggc gcatcagatc gttgaacccg gcatcaacca
    15301 gcacaaagtg gcggctaccc atttgtttga cgctccgcac ctgagtaatt aatacgccag
    15361 actgcgctac caggaagcga cccggttcaa tttccagttt cacagggtgg cccaaatggc
    15421 gagcgatttg ctcacgcgcg gcattccaca gaccataata atgttcggta tcaaccgcct
    15481 cttcaccctg ttgataagga atagaaagcc caccgcccgc agaaatagcc tgtaaatcct
    15541 gaccgaattc gataacctga cgcaccatag caccacacac ctgctccaga tgggcataat
    15601 caacgccaga accaatgtgc atgtgaatgc cgactagctg cagatgatga cgttgtatca
    15661 cgtccagtgc ggcgggcaga tcggtgtacc agataccgtg cttgctgttt tcgccaccgg
    15721 tattggtttt ttggctatgt ccgtgaccaa accccggatt aacgcgcagc catacccgat
    15781 gccctggcga aacctggccc agttggtcga gcgtatcaac agaacccgca ttcaccggaa
    15841 tttgcaattc actgacgcgt tcaagcgtcg cctgatcgat aacatctgcc gtaaaaacaa
    15901 tatcatcggg gtgcgtttgc ggattgtaac ccgccgccaa cgcacgctct atttcgccta
    15961 acgagacgga atccactttc acgccctgct cacgcattaa gcgcaaaata tgaatattgg
    16021 aacaggcttt ctgtgcaaag cgcaccactt caaactgttt cagcgctgca atctgccgac
    16081 gaataatttg cgcatcgtag acccacaccg ggcagccaaa ttcagcgggc aaacgcagca
    16141 gattttcggc ggtgagatcg gtatcggtgc tgaacagtga atgtggcata acaaactcca
    16201 gatgagtgct tttttatgat tacgccacat cataaaaaga ataaaaaata tcgatttatg
    16261 tcgagtctat gcaaaaatga tatggattac cggattgcga gagagcgcta atggccgccg
    16321 ttaacttacg tcatattgaa atttttcatg cggtaatgac cgccggaagc ctgactgagg
    16381 cagcacacct gctacacacc tcacagccaa ccgtcagccg cgaactggcg cgctttgaga
    16441 aggtgatcgg gctgaaattg tttgagcgcg tacgtggacg attacatcct accgtgcaag
    16501 gactgcgtct gtttgaagaa gtgcaacgat cctggtacgg actggatcgc attgtcagcg
    16561 ccgcagaaag tctgcgcgag tttcgccagg gagaactgtc tattgcctgc ctgccggtct
    16621 tttcgcaatc ttttttaccg cagctcctgc aaccctttct ggcacgttat cccgatgtca
    16681 gcttaaatat cgtgccccag gaatcaccgc tacttgaaga gtggctctcg gcccagcgtc
    16741 atgatttagg actcactgag acactccata cgcctacggg aacagaacgt accgaattac
    16801 tctctttaga tgaagtgtgt gtgttacctc cgggtcatcc gctggcggta aaaaaggtat
    16861 taacgccgga tgattttcag ggtgagaatt acatcagcct ttcccgtact gacagctatc
    16921 gccagttgct ggatcagcta tttactgaac atcaggttaa acgacgcatg atcgtagaaa
    16981 cccacagcgc cgcgtcagtc tgcgcaatgg tacgggcggg ggtaggtatt tcggtggtta
    17041 acccgctcac cgcactagat tatgcggcaa gcggtttagt ggtgcggcgg ttcagtattg
    17101 cggttccgtt caccgtcagc ctgatccgcc ccctgcaccg cccgtcatca gcgctggttc
    17161 aggcgtttag tgggcattta caagcggggt taccgaaact ggtcacttct cttgacgcta
    17221 ttttgtcgtc agctacgaca gcataaaagc gacagcatcc tcggcatgga tcgccgcggt
    17281 atcaaacaca ggcaaaacac tgcgctcttc tggcaccagt aaaccaattt ctgtgcagcc
    17341 aaaaatgacg ccctgtgcgc cctgttctgc aaggcgagca atcacttgcg cataataagc
    17401 gcgtgacgct tcggtaaatt gccccagaca cagttcttca aaaataatct gattaatttt
    17461 cgcccgttca tccgcttcag gaataagaca attgatggaa aattgttccg tcagccgccc
    17521 gcgataaaaa tcctgttcca tggtgtaacg cgtacccagc agcgccacac gagtcattcc
    17581 ggccccggta attgcacgtc cggtggcatc cgcaatgtgt aagaaaggca gagtgcaacg
    17641 tgactcaatg gcatccgcca ctttatgcat cgtattggta cataacacaa taccttctgc
    17701 gcccgcccgc tgtaagccaa gcgccgcctc agccagaatg tccccggttt tatcccattc
    17761 cccgcgacgc tggcactctt ctatttcatg aaaatcgacg ctatgtagca gcatttgcgc
    17821 agagtgaagc ccaccaagcc gctgtttaat gccttcattt atcagacgat agtaaggaat
    17881 ggtggattcc cagctcattc ctcccagcaa accaattgtt ttcattcctc tctcctgatg
    17941 tgtgttaccg caacaaggct gaataaggaa cgagacaaac gcctcaacgg ccaggtgccc
    18001 aatctctatt aacgaaaaaa gggccggatg tacagcacat ccggcccgtg aaatcagacg
    18061 ccgatatttc tcaacttctc gcctgccatc agtttgcgtt cgatatgttc cagcgtgaca
    18121 tttttggttt ccggaatgag ccagaaagta atacccacaa acgcaatgtt cagcgcagtg
    18181 tagagccaga acgtaccggc agcgccaatg ctatcaagca gtgtcaggaa ggccgcgccg
    18241 ataatcatat tcgacaccca gtttgtcgtc gtcgaacagg taataccgaa atcgcggcat
    18301 ttcagcggct gaatttcaga gcacaggatc cacaccactg gcgcggcgct catcgcataa
    18361 ccggcaatac acatcatcgt catgccaaca gagagccagg acaagccact ggaagccgta
    18421 ccgttatcaa actgcatcag gcaatagccc agcaccagag tgcctaacgc catcacgctg
    18481 aaaccaattt tcagagccgg tttacgacct gctttatcta ccgtaaagac cgcaatgaag
    18541 gtcgcgaaca taaaggtcag tcccacgacc agagtcgcaa tcatctgttg ttctgtggtc
    18601 gtaaagcccg ccattttgaa gatacgcggc gcgtagtaca tgatgatgtt cataccggta
    18661 aactgctgca tcgcctgcaa caacataccg agaaacacag cacgacggac gttacggttg
    18721 atcttaaaca gtgcccaacc gccctgtttt aacttcaggc tttcacgaat ttcgttgagt
    18781 tcttctcgcg ctttttccga cgtatcgcgc agcatacgca acacttcttc cgcctcaata
    18841 tgacgcccct tttccgccag ccagcgcggg ctatttggca ggaagaccac cagaataatc
    18901 agcagaactg ctggtaaagc aagaaccccc aacattgcgc gccagttacc gctataactg
    18961 aacgctgtat cggataaaaa cgccagcacg atgccgagtg tgaccatcaa ctggtacata
    19021 ctgatcatct taccgcgaac gttttcactt gccatttcag aaagatacag aggagcggtg
    19081 taagacgcga tcccgacagc aatgcccagc accacacgag cggcgattaa catctctacg
    19141 ctggtcgcaa aagcggaccc tatagaaccg agtacaaaca ggatggcccc cgccatcagg
    19201 ctgtatttac gccccaggcg gaacgacagc caaccattaa acagcgcacc aattgctgcg
    19261 ccgagcatca tgctactaac cacccattcc tgcaaacgac tggtcagcac aaagtgatcg
    19321 gtaatgaacg gcaacgctcc ggcgattacg ccgatatcaa gaccaaataa caatcctgcg
    19381 accgcagcag ctaccgaaac aaacatattc atacgccgcg tatcacgcaa agaacgtggc
    19441 gttaaagcag attccgtatt gatagtaacc attttttcct gccagcagag agtaagacat
    19501 tgtgaaaaaa tacgtgaaca attcacacag gtgtcaggtc ggaaacagca taaatatgga
    19561 ttaaattgct gcgacatgcc gttatgtgat ggatattcca attttaaaat taagttgaat
    19621 tattgagatt attattaacc tcctaatttt aaagcagata aaattcataa agttcattaa
    19681 ttgataattg atatggatta tttcataacc atgatatgga ttatgatgat ctagagatat
    19741 aaaaaaccct gccatgcggc agggtcataa aagtaagagg aatgaattaa cgcgccagcc
    19801 aaccgccatc cacagcaatg gtataaccat ttacataatc tgaagcgctg gaggcaagaa
    19861 acactaccgg ccccatcagg tcactcggca gtccccaacg gccagctgga atgcgatcga
    19921 gaatttccgc gctacgttgt tcatctgccc gcagttgttg ggtattgttg gtcgccatgt
    19981 aacccggagc tatcgcatta acattaatgt tgtgtttagc ccattcgttt gccatcaatc
    20041 gcgtcacacc catcacgccg ctttttgatg cggtataaga aggcacacgg atcccgccct
    20101 ggaaggagag cattgacgcg atattgataa tcttgccgcc attgccttgc gcgataaagt
    20161 gtttcgccgc tgcctgagac atgaagaata cgctcttgat attcaggttc atgacatcgt
    20221 cccagtcttt ttcgctgaac tcgagagcgt cttcgcggcg aatcaatccg gcgttattca
    20281 ccaggatatc aatatgacca aattcagcta ccgcgcgatc cagcagtcca ggaatgccat
    20341 caatctttcg cagatcggcg gtcaggctta aaaaacgacg ccccagcgcc gtgacctgct
    20401 cgatggtttc agtcggttca acgatgttaa tgccaacaat gtcacagccc gcttgcgcca
    20461 gccccaacgc catcccctgg cccagcccag tatcacaacc agtgacgacc gcaactttac
    20521 cttggagaga aaatgcactt aaaatcataa caacacctta ttcgtttatg cccacaacta
    20581 gcgcaaatct ttaacgccca catggtccat atcattaaag acctggtttt caccgaccat
    20641 gccccagata aaggtataag ctttggttcc gacgccggaa tggatcgacc agctcgggga
    20701 gatcaccgcc tgctcgttat gcatcacaat gtgacgcgtt tcttgcggct gccccatcat
    20761 gtggaaaacg caggcgtcat catccatatt gaaatagaaa taaacttcca tccggcgctc
    20821 gtgggtgtga cacggcatgg tgttccacaa gttacctgga gccagctccg tcagccccat
    20881 actcaattga caggtttcca gtacatccgg gacaaaatat ttgttaatcg tgcgacggtt
    20941 actggtgagg ttatcgccta acgtgactgg agatacttcg tccggtgtga cttttttggt
    21001 gggatacgtc gtatgcgcgg gtgcgcaatt gtaataaaac ttcgccggag tggcggtatc
    21061 aatactggca aagacaactt cttttgcacc tttaccaaca tacagggcgt cgcggtgacc
    21121 gatttcatag cattgaccat cgacagtaat cgtaccggca ccgccaatat tgataacacc
    21181 taactcgcga cgttcaagga aatagcttac gccgagttgt ttaccaactt ccccgccaac
    21241 ggaaaccgtt ttagttatcg gcataatgcc gccaacaata attcggtcaa tgtggctgta
    21301 aaccatggtg tactcatcgg cgacaaatac cttttcaacc aaaaattcat tgcgcagccc
    21361 ttgggtatcc agcgtttttg cgtgcgcacc gtggatgctc tgtcttacgt ccacatcaac
    21421 ctccgataat cagtgaagcg tgaaaataga aaacgatagt gaaacactgt ttcgttttct
    21481 ctataaaggg atgatatgcc catgggctag ggttttcaat taaaaataaa acaatgtttt
    21541 atttttaatt taaggattaa aaaaagtgca gtgtcgatca cgaatatgtc atgagaatgg
    21601 aaaaatgagg gagcccagaa aatgtatgtc gacttgccag ccatcattat tcaacagatt
    21661 aatgacggca gatatttatc ggtaaggagg aaatgcagag ctactcgtca cgttcaatgg
    21721 tcaatgccac gccctggccc ccgccgatac aaagcgttgc cagccctttg cgggcattac
    21781 gtttcaccat ttcatgaacc agagaaacca ggattcggca accggaagcg cctatcgggt
    21841 gaccgagtgc gatcgcgcca ccattgacat tgacccgacg ctcatcccac tcaagcatct
    21901 tgccaaccga aagcgcctgt gcagcaaacg cttcattagc ctcgataaga tcgacatcag
    21961 ccaactgcca gcctacacgc tccaggcaac ggcgggtcgc atacaccggc gcaattccca
    22021 tcaatgccgg atctacacca acgctggcaa atgcgcggat gcgggccagc acgggtaaat
    22081 tcaacgctcg tgctttggct tcgctcatca tcattaccgc agctgcgcca tcgtttatgg
    22141 atgatgcatt ccccgcggtc acagaaccga gactatcaaa tgaaggattt aaacgggcta
    22201 aaccttctgc actggcgtca gtgcgtggct gttcatcggt atcaacaacc aacgtctgac
    22261 cgttactttg gatcattacc gggacgatct catctttaaa tcgtccggcg tcaatcgccg
    22321 ctcgcgcttt ttgttgcgag ctaagtgcgt aagcatcctg caactgacgg ctgatgccat
    22381 attcgcgagc cagattttcg gcggtgacac caatatgata atcattgaag gcatcccaca
    22441 acccatcatg caccagactg tcaaccaact ggctattgcc aagctgttca ccggtgcggc
    22501 tatcagtcag aacatgtggt gcgcggctca tgttttcctg gccacctgcg atgacaatat
    22561 cagcctcgcc acactgtatc gcctgagtag ccagatgcag tgctttaaga ccggaaccgc
    22621 aaacgtcatt aatcgtgatt gcagaaacgc tgttaggaag accaccttta atagccgatt
    22681 gccttgccgg attctgccct gcccctgcag tcaacacctg accaagaatt acttcatcca
    22741 ccgcatatgc aggaacgccg gtacgttcta ttaacgcttt cacgaccaga ctaccaagtt
    22801 ccacggcgga atgacccgct aacgcaccac gaaagcagcc gataggtgtc cgtaacgccc
    22861 cgacaatcac aacgtctttc atcacaacct cgcaccaatt aacagtgcaa tagtaaatga
    22921 ttgttaatta cagttatctt aattgtttaa aaaaagtgat ttttatcaca aaggaaatat
    22981 gcctgagcag tagtcagagt cataactggc acgtaaggtt tgcaaccact aacccaccaa
    23041 tagatgggta gatagggcgt taatctccca tacttaactt ggtttatggt aaattgccct
    23101 ccattttgtt taatttgtag atgatacgtt cagataatgt ctaatatttg gtctaaagaa
    23161 gaaactctgt gaagtttcgc gctctatggc acagccgttg atgcaggcac gctattcctt
    23221 cctattcagt taggttcggc aggggctgtg gtcctgttta ttactgctct ggtcgcctgg
    23281 cctttaacat attggccaca taaagcctta tgccagttca tcctctcatc aaaaacatca
    23341 gcaggtgaag ggataacggg cgcggtaaca cactactatg gcaagaagat tggtaatctg
    23401 attaccacgc tgtacttcat cgcctttttt gtcgtcgtgt tgatatatgc agtggcaatt
    23461 accaactcac ttacggaaca gctggcaaag catatggtta ttgatcttcg catccgtatg
    23521 ttggtgagtc tgggtgttgt attaattctg aatctcattt ttctgatggg acgtcatgcc
    23581 actattcggg taatgggatt tttggtattc ccattgattg cctatttctt atttctttcc
    23641 atttaccttg tcggtagttg gcaacctgat ctattaacaa cccaggtaga gttcaatcag
    23701 aatacccttc accagatatg gatatcgatt cccgtgatgg ttttcgcctt tagccatacg
    23761 cccattattt ctacgtttgc catagacaga cgtgaaaaat atggcgaaca cgctatggat
    23821 aaatgcaaaa aaattatgaa agtcgcttat ctcatcatct gcataagtgt actgttcttt
    23881 gtctttagct gcctgctttc tattccatct tcgtatattg aagcggctaa agaagaaggg
    23941 gtcaccattt tatcggcgct ttctatgctg ccgaacgccc cagcatggtt gtcaatttcc
    24001 gggattattg tcgcagtagt tgcgatgtcg aaatcattcc tgggtacgta ctttggcgtt
    24061 attgaaggtg ccacagaggt cgtcaaaaca acactacagc aggttggtgt aaagaaaagt
    24121 cgtgcattta accacgcact atcaattatg ttggtatcgc tgattacctt cattgtttgt
    24181 tgcattaacc cgaacgcgat ttcgatgatt tacgcgatca gcggcccgct cattgccatg
    24241 atacttttca tcatgcctac gctgtcaacg tatctcatcc cggcgcttaa accctggcgt
    24301 tccatcggaa atctgattac gctgatcgtg ggtatcctgt gcgtatcggt aatgttcttt
    24361 agctaatatc cgttgtggat ggttcaaaat tgctacaatt ttgaaccatt ccatttttca
    24421 taatattcat ttgttattat tccgccctat cacttctctc gtcatatcct agaattatca
    24481 gttagttaaa cacacaaaga gatctgaaca tcttaaataa gactatttaa gatgcataac
    24541 ttagattcgc aagatatctt ctggtcattc aaaaacaatt tgcgatatag atattctcat
    24601 accatgagat agttatctgg accacgttcg accatgcaag attaaatcag tgaaacataa
    24661 tcatatttaa taacgagaaa tgcattttta aatatcttaa tcagaattat agcaaataca
    24721 gattaatcgc aatttttccc agcaaaatta caaaatcaag gtgtattgac atcatcaatc
    24781 accaatgaaa ttatgcaatt atatacggat agggaggttc ttaacatggg gctttgtagt
    24841 cgttataaaa gtcttacatg caatagttgc tcaatgcatt gccaaataat gccagaagag
    24901 tcaccgcgtt tgcagtactg cgcaaactcg tgttttgatc ttacccagca atagtggaca
    24961 cgcggctaag tgagtaaact ctcagtcaga ggt
//
LOCUS       DADLTY010000049        24181 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.049, whole genome shotgun sequence.
ACCESSION   DADLTY010000049 DADLTY010000000
VERSION     DADLTY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.049"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(22..594)
                     /locus_tag="JN334_001659"
     CDS             complement(22..594)
                     /locus_tag="JN334_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437827.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="JN334_001660"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="JN334_001660"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HAY7437828.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="JN334_001661"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="JN334_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HAY7437829.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="JN334_001662"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="JN334_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAY7437830.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="JN334_001663"
     CDS             complement(3556..5337)
                     /locus_tag="JN334_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY7437831.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="JN334_001664"
     CDS             complement(5330..7102)
                     /locus_tag="JN334_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY7437832.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7677)
                     /locus_tag="JN334_001665"
     CDS             complement(7132..7677)
                     /locus_tag="JN334_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAY7437833.1"
                     /translation="MVFKPHRINCRDFTFKIELKEKKILRGRAMLDKIDRKLLALLQQ
                     DCTLSLQALAEAVNLTTTPCWKRLKRLEDDGILIGKVALLDPEKIGLGLTAFVLIKTQ
                     HHSSEWYCRFVTVVTEMPEVLGVWRMAGEYDYLMRVQVADMKRYDEFYKRLVNSVPGL
                     SDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="JN334_001666"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="JN334_001666"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HAY7437834.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8780..10354
                     /locus_tag="JN334_001667"
     CDS             8780..10354
                     /locus_tag="JN334_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HAY7437835.1"
                     /translation="MLRQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALE
                     TGKQQDVLELAQLAPGELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAE
                     QHLAGQIFSGLTRFDNNTQRPIGDLAHHWETSTDRLRWDFYLRSTLHWHNGDAVKASH
                     LHQRLLMLLQLPALDQLFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQ
                     FPLIGTGPFRLTQFTAELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPV
                     QITIGKPEELQRVSQVSSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEV
                     GENLITASHALLPGWTIPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGC
                     ELTIIFHNAKNWDDTTLLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYA
                     HLQSTLDAVQVMPDEENRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTP
                     RGWFEFTEAWLPAPSQ"
     gene            10419..11114
                     /gene="queC"
                     /locus_tag="JN334_001668"
     CDS             10419..11114
                     /gene="queC"
                     /locus_tag="JN334_001668"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAY7437836.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11166..11564)
                     /locus_tag="JN334_001669"
     CDS             complement(11166..11564)
                     /locus_tag="JN334_001669"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HAY7437837.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11658..12029)
                     /locus_tag="JN334_001670"
     CDS             complement(11658..12029)
                     /locus_tag="JN334_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437838.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="JN334_001671"
     CDS             complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="JN334_001671"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY7437839.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14243..14515)
                     /gene="hupB"
                     /locus_tag="JN334_001672"
     CDS             complement(14243..14515)
                     /gene="hupB"
                     /locus_tag="JN334_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HAY7437840.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14724..17078)
                     /gene="lon"
                     /locus_tag="JN334_001673"
     CDS             complement(14724..17078)
                     /gene="lon"
                     /locus_tag="JN334_001673"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAY7437841.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLASLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="JN334_001674"
     CDS             complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="JN334_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HAY7437842.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="JN334_001675"
     CDS             complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="JN334_001675"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAY7437843.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19536..20834)
                     /gene="tig"
                     /locus_tag="JN334_001676"
     CDS             complement(19536..20834)
                     /gene="tig"
                     /locus_tag="JN334_001676"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAY7437844.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="JN334_001677"
     CDS             complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="JN334_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HAY7437845.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21800..22378
                     /locus_tag="JN334_001678"
     CDS             21800..22378
                     /locus_tag="JN334_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7437846.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22422..23897
                     /gene="ampG"
                     /locus_tag="JN334_001679"
     CDS             22422..23897
                     /gene="ampG"
                     /locus_tag="JN334_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HAY7437847.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5666 a         6665 c         5827 g         6023 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacagtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattt cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccactgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctcc gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgggttcgct accatcgttc accaccggcg cttccgccag catggcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctggacgcgc atcagataat cgtattcacc agccatgcgc cagaccccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctac cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg
     8701 tggcaacctt cagccgcaaa ccgtcaccgt cagcgaactg gccgaacgct gtttttgcag
     8761 cgaacgccat gttcgtacgc tgttgcgtca ggcacaggag gcgggatggc tggagtggca
     8821 ggcgcagtca ggacgcggaa agcgcggaca attacgcttt ctggtcacgc cggaatcgct
     8881 acgcaatgcg atgatggaac aggcactgga aaccggaaag cagcaagatg tgctggagct
     8941 ggcgcaactg gccccaggtg agctgcgcac tctgttacag ccatttatgg gcggacaatg
     9001 gcaaaacgat acacccacgt tgcgtattcc ctactatcgc ccgctcgaac cgctacaacc
     9061 aggctttttg cccggccgtg ccgagcagca tctcgccggg cagatatttt ccggcctgac
     9121 ccgcttcgat aataatactc agcgcccgat tggcgattta gcgcatcact gggaaacctc
     9181 tactgacagg ttacgctggg acttttatct tcgttcaacc ctacactggc ataacggcga
     9241 tgcagtaaaa gcctcacact tacaccagcg attattgatg ctgttacaac tgccagcact
     9301 ggatcaatta tttattagcg tgaagcgtat tgaagtcacc catccgcagt gtctgacctt
     9361 ctttttacat cgccccgatt actggcttgc gcaccggctg gcgagctatt gcagccatct
     9421 ggcgcatccg caattcccac tgatcggcac tggtcctttt cgcttaacac aattcacagc
     9481 ggagctggtg cgcctggaga gccatgatta ttaccatttg cgtcatccgc tgcttaaagc
     9541 ggttgagtac tggataactc cgccgctttt cgaaaaagat ttgggaacca gttgtcggca
     9601 tcccgtgcaa atcaccatcg gcaaaccgga ggagctgcaa cgggtcagcc aagtcagtag
     9661 tggcatcagt ttaggttttt gctatttaac gttgcgcaaa agtccccgac tctccctctg
     9721 gcaggcgcga aaagtgatct ccattattca tcaatccggt ttattacaaa cgttagaagt
     9781 cggagaaaac ctgatcaccg ccagtcatgc attactgcca ggctggacta ttccgcactg
     9841 gcaagtaccg gatgaagtca aactaccgaa aaccttgacg ctggtttatc acctaccgat
     9901 agaacttcat accatggcag aacgcctaca ggcgacactg gcagcggaag gctgtgaact
     9961 cacaattatt tttcataacg caaaaaactg ggacgacacg accctactgg cacacgcaga
    10021 cctcatgatg ggcgacagat taattggcga agcaccggaa tatactctgg agcaatggct
    10081 acgttgcgat ccactatggc cacatgtttt cgacgctcca gcatatgcac atctgcaatc
    10141 gacgctggac gcggtgcaag taatgcctga tgaggaaaac cgatttaatg ccctgaaagc
    10201 ggtttttagc cagttaatgg cagacgcgac gctgacgccg ctgttcaact atcactatcg
    10261 cattagtgcc cctcccggcg tgaacggtgt gcgactgaca ccgcgcggct ggtttgaatt
    10321 taccgaagcc tggcttcccg cgccgtcgca atgaatagct ggtccgggtt aatcgcaggc
    10381 gttaccataa cgcttttatt gttcaagcag gattatctat gaaacgtgct gtcgttgtgt
    10441 tcagtggagg ccaggattcc accacctgtc tggtgcaggc attacaacaa tatgatgaag
    10501 tccattgcgt gacgttcgat tacggtcagc gacatcgcgc agaaatcgac gtggcacgcg
    10561 aactggcgct gaaactggga gcacgcgcgc ataaggttct tgatgtaacg ctgctcaacg
    10621 agctggcggt cagcagcctg acacgtgaca gcattccggt gcctgattat gaacctgaag
    10681 ccgatggcat cccgaatacg tttgtcccag ggcgtaatat tttgttcctg acgctggcgg
    10741 caatatatgc gtatcaggta aaagcagaag ccgtaattac cggcgtctgt gaaacggatt
    10801 tctccggcta cccggattgc cgcgatgagt ttgtgaaagc actaaaccat gccgtcagtt
    10861 tgggcatggc gaaagatatt cgttttgaaa cgccgctgat gtggattgat aaagcggaaa
    10921 cctgggcgct ggcagattat tacggcaaac tggatttagt ccgtaacgaa acgttgacct
    10981 gctataacgg cattaaaggc gacggttgcg gtcattgtgc ggcatgtaat ttacgcgcca
    11041 acggtttgaa tcattatctg gccgataaac cgacggtgat ggcagcgatg aagcagaaaa
    11101 ccgggttgag gtaattattc cgggtgtcgc cggatgcggc ttgagcatcc ggcaccacaa
    11161 aacgtttact taaccatctg ctccagcttt tcgcgcaatt ccccttccag agctaatgct
    11221 ttctgcgttt taagatcaat acaaacaaac gtaataagcg catccgctac cacctgccct
    11281 tccggctcca gtgtaatgac ctggtttaag atgccgcttt taccgtttaa ttgctgcaac
    11341 tgactggtaa tagttaacag gtcgcttaat accgctggac gacgatagtt aatattgata
    11401 ttgaccacga cgaaggcgat gttatgggcc gtcatccact gaaaactgtc gctattttcc
    11461 accccatccc agcgggcttc ttcgagaaat tcaaggtagc gggcgttgtt gacgtgctgg
    11521 taaacgtcga gatgatatcc acgaactttg atttgtgttt gcatagcgca ataaccttac
    11581 gttgttgtta taagtacaga ggtcataact ggtatgacct cttcagtctg gcaaaaaatt
    11641 gccactatgc aaattaatta cagggttaat accgccagat tacgttccac cagcgaattg
    11701 cccatccccg gcacctgctt tagatcctcc accgttttaa acggaccgta ctcttcgcga
    11761 taactgacaa tcgcctgcgc tttcttcagg ccaacgccat tcatcgcgcg ggctagctct
    11821 tccgcgctgg cattattaat gctgacccgg gtgccttctt cgtcactggc tttcgccggt
    11881 actgctgctt tactttgtgc tgcaggagct tccgctttgg tttctaccgc cgtcggtttc
    11941 gccacagaag ctgccgccag cgcgctatga gacattccgg cacaggccag ggacagtgta
    12001 atgagcagtg ctttaattcc gtgtttcata ctgttttctc cttgtttgtt aacagtggaa
    12061 tcaccttaac gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg gaaaaggccg
    12121 cgaaagcgac cttttcttgt tacagctgca acgcattttt ttgcgaggcg gcttcaggat
    12181 tattgctgtt ccagcgcatc gccaattttg attttcgcct ctttacgcag gttactcatc
    12241 agagcttcaa agacgatttg tgcgttgttc tgggtgatac cctgcaccat cgcttttttc
    12301 tgatcttccg gcattgaacc ttgtttcact tcatccagcg ccagcagaac cacattacct
    12361 tgcatatcgg tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg cagtgcaaac
    12421 gccgcctggc taatcgggtc acgaccggaa cggcttaagg ttttcggctc gccaaatttc
    12481 agaccggcag cctgcatagc ttccgcacct ttgccggctt tcaaatcaac cagcagtttc
    12541 tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc cttaacttgt
    12601 tcctgaacat ctgccaacgg tttcaccgct tccggtttgt gctcgctgac gcgcagcacg
    12661 aatgcgcggt cgccgtctac ggtgatgatg tcagagttga tgcccgacgc gccgttttca
    12721 cctaccagac cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa ctcttccggc
    12781 aggttatctt tgctgaacca acccgtctga gtggctttaa cgccggcagc ttgctctgca
    12841 ccggccagag actcggtgtc gttgcttgcc gcatcgctca ctttctgctg cagcgcatag
    12901 tacgcatcga gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg tacttcgtct
    12961 aacgatttca ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa accgaccgaa
    13021 gatttgatga caccagacag ttggcctttt tctttcagac cagcattttt cagttcgtcg
    13081 gggatagtgg catcttctaa ccaacccata tcgccgccgt tacgggcaga gataatatcg
    13141 gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc atcaagtacc
    13201 gctttcgctt catcttcagt tttggtctgg atgatgctgt agcgggtacg ctgcggctgg
    13261 gtgaattgat cctgatgctg atcgtagtag ctctggatat ccgcatcgct aaccggttgc
    13321 tgcatcgttg cggcaaccag cttgatgtaa ctcacgcgga attgttccgg cgtcatgaaa
    13381 ttgtttttgt tttgttcgta gtagctggca atctcctgtt cggtcacagg ctgcttcgcc
    13441 gccagcgcgt taacatcgat agtcgcctca cgcaccacgc gttgttgcgc gaccagtgcc
    13501 gccagctcgt cggtttcacc tttcagcata aaatcggtac cggcaacgcc gttaatcagc
    13561 tgttgggtag tgagctggtt acgcagcgcc tgggcgtact gatcggcggt catccccatc
    13621 tggttgagga taccgttata gcggctgtta tcaaatttgc cgtcaacctg gaaggctggg
    13681 gtcgcgaaaa tcgcctgttt aacctgctca tcactgatac ccagtttcag ctcacgagcg
    13741 tactgatcca acagcgcctc gtcgatcaga cgattcagca cctgttgacg cagggttttc
    13801 atatagcctt cgttcgctgc cagctcggag tattgatcgc ccagctgttg ctgcatacga
    13861 ttacgctcgc tgttaaaggc gttctcgaac tgcccacggc tgatttcctg gtcattcact
    13921 tttgcggcgt agttattgcc tccgccaatc aggtaaccac tcacgccggt caatatgaac
    13981 gacacgataa tgataccgaa aataatcttg agcacgagac tgtttgcagc cgtgcgtaag
    14041 ctgtccatca tggtgtaaca acactccgct gtagatgact gtgtacctca cgcaacgtat
    14101 cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca attgtcagcc cacaagacac
    14161 agaaagtcac cgaatacaaa taaaaaaggc acatcagtag atgcgccctt gaacttcgtc
    14221 acatccccac tggggacaac gcttagttta ccgcgtcttt cagtgcttta cctgcacgga
    14281 agctcggtac tttagcggca gcgatggtga tctctttacc ggtctgcggg ttgcggccag
    14341 tacgggcagc acgctcttta acggcaaaag taccaaaacc taccagtgct acatcatccc
    14401 cttctttcag agattcagtt acggaagcaa taatagcatc taacgcacgg ccagccgcag
    14461 ctttagagat atcagcccct gcagcaatct tgtcgatcaa ttgagattta ttcactcttc
    14521 tcttcctctt tataatttat atcgcacttg aatccttcaa ggtacgaacg cgcagcagtt
    14581 atatcaggcc agccatcccc ttacaacacc ccttaataag ggcaagcccg atccgctatc
    14641 taacttagcg agacaaaaaa aggctggcaa gcccgaatta gcctgccagc cctgttttta
    14701 ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc agacggttca
    14761 ttttgcagcg ccagagtcag aacttcctca atgcgcttca caggatgaat gtccagatcg
    14821 gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg aattagcact
    14881 gttttaatcc cgccgcgatg cgctgccagg agtttttctt tcaaaccacc gatcggcagt
    14941 acctgaccac gcagagtgat ctcaccggtc attgccacat cggcacgaac cgggttaccg
    15001 gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg cactcggacc atctttcggc
    15061 gtcgcacctt ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc agggttgatc
    15121 cccagttttt ccgcacgcgc acgaaccacc gttaacgccg cctgaataga ctcctgcatc
    15181 acttcgccga gcgaaccggt ataggtcagt ttgcctttac ccggaacaca tgcggtttca
    15241 atggtcagca agtcaccgcc cacttccgtc cacgccagac cgattacctg accgacacgg
    15301 ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc atgcaggtta
    15361 tcgccgttaa tttcgatatg ttttaatgac ttatcgagca gtaactgctt aaccgctttg
    15421 cggcacagtt tggagatttc acgctccaga ccacgcacgc ccgcctcacg ggtgtagtaa
    15481 cgaataatgc cgataatggc gctatcgtcg acggtcagct cacctttttt cagtgcatta
    15541 cgttcaatct gcttcggcag caggtgacgt ttggcgatgt tcagtttttc atcttcggta
    15601 taaccggaga ggcgaatcac ttccatacga tccagcagcg gtgccggaat gttcatggag
    15661 ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat ccacttccag gtagtggtcg
    15721 ctgaacgcta cgttctgctc tggatccagc acttcaagca gtgcagaggc cggatcgcct
    15781 cgcatgtcag aagacatttt gtcgatctca tcgagcagga acagcggatt tttcacgccc
    15841 actttcgcca ttttctggat cagtttaccc ggcatagaac cgatgtaagt acggcggtga
    15901 ccacggattt ccgcttcatc acgcacgccg cccagcgcca tacggacata tttacgcccg
    15961 gtggctttgg caatggactg accaagagag gttttaccta cccccggcgg ccctaccagg
    16021 cagaggatcg gtcccttgat tttgttgaca cggctttgaa ccgcaaggta ctcaaggatg
    16081 cgatctttca cgcgctccag accataatgg tcggtatcaa ggatttcctg cgcctgacgc
    16141 aggtcttttt tgaccttgct gcgcgcattc cacggtacct gtaccatcca gtcgatataa
    16201 ccacgcacta cggtcgcttc tgccgacatc ggagacatca ttttcagctt ctgcaactct
    16261 gcttccgctt tctcttttgc ctctttcggc atcttcgccg cgtcgatttt gcgcttcagg
    16321 gcttcgtttt cgtccggcac gtcgtccatt tcaccgagtt ctttctgaat agctttcatt
    16381 tgctcgttca gatagtactc acgctgggat ttctccatct gctttttaac gcggttgcga
    16441 atgcgtttct caacctgcag cagatcgatt tccgattcca tcattgccat cagatattcc
    16501 agacgttcgt taacgtcgga catctccaga acggactgtt tgtcagccag tttcagcggc
    16561 atatgtgcag caatggtatc cgccagacgc gccggatcgt cgatgctatt cagcgacgcc
    16621 agcacttctg gtgggatttt tttgttcagc ttgatgtagc cttcgaactg gctgattgca
    16681 gtacgcacca gcacttcctg ttcccgctca tcaatggtcg gcgactccag atactccgcc
    16741 ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg ctgtaacccc
    16801 tcgaccagca ctttgacggt gccgtcaggc agtttcagca tctgcaatat agaggccacg
    16861 gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc tttctgcgcg
    16921 accagcataa tttttttatc atggtccatc gccgcttcca gacaacggat agatttttcc
    16981 cgcccgacaa ataaggggat gaccatgtgc ggataaacca ccacatcgcg cagcggcaat
    17041 acggggattt caatgcgttc agaacgctca ggattcatag agctctctct tagtttaatt
    17101 tccgccaggt aatcagatga cacgactgtg cttcacgcca tctattaaca tgtacgtcag
    17161 tatatgggga tgtttccccc acattcaacg ccgagaatag aggaaaaatt aaaggggaga
    17221 taaaatcccc cctttttggt taactaattg tatgggaatg gttaattatt caccagatgc
    17281 ctgttgcgct tccggcttac cataaatcag caacggtttg ctttgaccat caattaccga
    17341 ctcgtcgata accacttttt cgacgtcttc catggacggc agatcgtaca tggtatcgag
    17401 cagtgcggct tctacgatgg aacgcaggcc acgggcaccg gttttacgcg ccatcgcttt
    17461 cttagcgata gcatccagcg cctcgtcacg gaattccaga tccacgcctt ccagattaaa
    17521 cagcgcctga tactgcttgg tcagggcgtt tttcggctct ttgaggatct gaatcagagc
    17581 ttcttcgctc agttcattca acgttgctac aaccggcaga cgaccaatga actcagggat
    17641 aagaccaaac ttgatcagat cttccggttc aacctgcgcc agcagctcgc cttcgcttgc
    17701 tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg tttctacacg
    17761 gtgggaaatc actttatcca gaccggcaaa cgcaccgcca caaataaaca ggatcttaga
    17821 ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg gtggaacagc
    17881 agctaccgta ccttcgatca gtttcaacag tgcctgctgt actccttcac cggaaacgtc
    17941 tcgggtaatg gacgggttgt ctgacttacg agaaatcttg tcgatttcat cgatgtagac
    18001 aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt tgcaacagct tctgaatgat
    18061 gttttcaacg tcttcgccca cataaccggc ttcggtcagc gtggttgcgt cggccatggt
    18121 gaacgggacg tccagcaggc gcgccagcgt ttcagccagc agcgttttac cggaaccggt
    18181 cggaccgatc agcagaatgt tacttttgcc caactcgaca ccattgctgg tatcgccgtt
    18241 gcgcagacgt ttgtaatggt tgtataccgc gaccgccagc acttttttcg cctgttcctg
    18301 gccgataacg taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta gcgcactgcg
    18361 ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac ataaatcaac
    18421 acattcgtcg cagatataca cggatggacc ggcaatcagc ttgcgcactt catgctggct
    18481 tttgccgcaa aaagagcaat acagcaattt gcctgagcca tctttgcgtt tatctgtcat
    18541 gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg ccattctcag
    18601 gcgcaagccg cttacagcgt tgtgccgccc tagataagta tagcggcaca gttgcgcctc
    18661 tggcatcaat tacgatgggt cagaatcgaa tcgaccagac cgtattccac cgcttcaggg
    18721 gcggaaagga agcgatcgcg ctcggtatca cgttcaatct gttctaatga ttgacccgta
    18781 tgaagcgcca taagttcatt catgcgccct ttaactttca gaatttcacg ggcatgaatt
    18841 tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat cacgcgcgaa
    18901 ttcggcaggc aaaaacgttt accttttgcc cctgcggtca acaagaaagc gcccatcgag
    18961 gccgcctggc ccatacagat ggtgctgaca tcaggcttga taaactgcat ggtgtcatag
    19021 atagacatcc cggcagtgat caccccgcct ggggagttaa tgtacagata gatatctttt
    19081 tctgggtttt ccgcttccag gaacagcatc tgcgccacaa tcaggttagc catgtggtct
    19141 tcaacctggc cagtcagaaa aatgacgcgt tccttaagta gacgagaata gatatcaaaa
    19201 gagcgctcac cgcgtgaggt ctgttcaatg accatcggca ccagcgccat atggggtgca
    19261 aagttatctc gttcgccgct gtatgacatt tccgtctcct ggataaaatt gaaaaaaacc
    19321 tgctgtaccg attgtaacct tatggacgga ttatcagcta gtccctgtgt tatgggtacg
    19381 gcatcaccat atttcaagca taacaatctt ttgttgttac gctaacaccg aaagaggctt
    19441 ttcgcacggc tccatgcaaa attcatgaca aaaaaaagcc caccacctgg cggtgacggg
    19501 cctttgtgcg aatttagcgc gttatgctgc gtaaattacg cctgctggtt catcagctcg
    19561 ttgaaagtgg tttctttttc agtcactttc gctttcgcca gtacagcttc aacagcctgt
    19621 tcttccagag caacattgcg catgttgtcc atcagttctt tgtttttgct gtagaactcg
    19681 ataacttctt tcggatcttc gtacgcagaa gccatctctt cgatcaggcc tttcacgcgc
    19741 tcttcgtcag ctttcagctc gttggtgcgg ataacttcgc ccagcagcag gccaacaact
    19801 acgcggcgtt tagcctgttc ttcgaacagt tcgcgcggca gttccagagc ttgtttttcg
    19861 ttgccaccga aacgctgtgc agcctggcga cgcagaacgt cgatttcgct gtcgatcagc
    19921 gcagccggta cgtcgatgtc gttagctttt accagacctt cgatcgcctg agacttaacg
    19981 cggttacgga tggcgctctt cagctcgcgc tccatgtttt tacgcacttc agcgcgcaga
    20041 ccttctacgg aaccatcttc aacgccgaaa cgtttgatga attctgcagt cagttccggc
    20101 agttcacgct cttcaacttt cttcaggttg atagcgaatt tcgctgcttt acctttcagg
    20161 ttttctgcgt ggtattcttc cgggaaggtc acgtcgatgg tgaactcttc gccagctttg
    20221 tggcctttga taccgtcttc aaagcccggg atcatacgac cctggcccat cgccagtacg
    20281 aaatcagacg ctttaccgcc ttcgaactct tcgccgtcta cagaaccggt gaagtcgatg
    20341 gttacgcggt cttctgcttc aacagcgccg tctttttctt tccaggtcgc ctgctgttta
    20401 cgcagagtat ccagcatgcc gtcaacgtca gcgtcggtca cttcaacgat cggtttttca
    20461 acttcgatcg cttccagacc ctgcagttca acttccggat aaacttcaaa ctctacagag
    20521 taagtgaagt cttcacccag cttgtattcg cccggaacat aagtcggtgc gccagccgga
    20581 ttgatttttt ctttaatgat ggcgtcaatg aagttacggc tcatcaggtc acccagaacg
    20641 tcctggcgta cagacgcgcc ataacgctga gcaacgatat tcattggcac tttgcctttg
    20701 cggaagccgt caatacgtac ttttttcgca acgttgacca gctcgctttt aacagcggtc
    20761 tcgatgctgt cagcagcgat agtaatcgtt acacggcggc caaggccttg agtggtttca
    20821 actgaaactt gcatcttgtt acctcaaaaa atcacagtgc tcggtcaact cttaccccgc
    20881 aagcacaggt tgttggcttc gcggaaccgg gatgttctta taatcaatca cattccctgt
    20941 taccagaatc atcccgaaag cattcaaata ggacgcggca ttatagcggc atcactttta
    21001 tgagtcgaga acggttattg cgcgctgtcg cgtatttttt cacaattccc gggcaatttt
    21061 cgtctgaatg ctgggcaaaa agcgcaaaat ccagacaaaa caaaacggcc cgcaggccgt
    21121 ttttcataaa atctttagca acatactgga aaagcgccga cagttgcaaa tgcgttttta
    21181 cgcgatgctt cctgctccac gacagggagg agaggcaaag acggtgtcct gcaacccttc
    21241 ccactcctta atagtgtaag tatgcagagc cagcgcatga acggtagtag agagttcctc
    21301 cgctaaagta ctgtaaatca ttcgatgacg attcagaaaa cgttcacccg taaaacgatc
    21361 gctgaccagc acaactttaa aatggctttc agagccggct gggacattgt gacgatagct
    21421 ttcatccact acttcgagga atacgggttg gaacgccgcc cttaattttt cttctatccg
    21481 ctcacgtatc atcatgaaat atctcctccg acaacgctga gatgtcatcc atccctttaa
    21541 atactagccg cttttaccgt ttccattgca gcttaacaac aaatatttag gtttcgtcct
    21601 ggtttttccg tcaagcgtat gaagtttact gatttaaaga ttgaaacaca ggcctcgcat
    21661 cagcgtttct tttaccaggg gtgattattt cgtcaacatg atgaatttac atgccttacc
    21721 cacttcccct cgccgttggc aatgttatga tggcgggaat ttttaccaaa cacgcttaag
    21781 agtctctgag agcccgaaca tgttcaaaaa aatcctcttc ccgttagttg ctctgtttat
    21841 gcttgcagga tgcgcaaaac cgccaacaac tattgaagtt tccccgacga ttacactgcc
    21901 acagcaggat ccaagcctga tgggcgtcac cgtaagcatt aatggtgccg atcagcgtac
    21961 cgatcaggcg ctggcaaaag tcacccgcga taatcaaatc gttaccctga ccgcctcccg
    22021 cgctctgcgt ttcctgctgc aagaagtgct ggaaaaacag atgaccgcgc gtggttacat
    22081 ggttggtccg aatggcccgg ttaatctgca aatcatcgtt agccaactgt atgctgacgt
    22141 gtcccagggc aacgtgcgct acaacatcgc taccaaagcg gatatcgcca tcatcgctac
    22201 cgcgcagaat ggcaacaaaa tgaccaaaaa ctatcgtgcc agctacaacg ttgaaggtgc
    22261 gttccaggcc tccaacaaaa atatcgccga tgcggttaac agtgtgctga ccgataccat
    22321 cgctgatatg tctcaggaca ccagcattca cgaattcatc aagcagaacg cgcgttaatt
    22381 tctgccctct ggcccggtgc aagccgggcc tgtagacgcc catgtccagt caatatttac
    22441 gtatttttca acagccgcgt tcagccatat tgctgatcct gggttttgct tccgggctac
    22501 cgctcgccct gacatccggc accttacagg cctggatgac ggtcgaaaat atcgatctca
    22561 aaaccattgg tttcttctct ctggtaggcc aggcttacgt ttttaaattc ctctggtcac
    22621 cgctgatgga ccgctacacg cccccatttt ttgggcggcg gcgcggttgg ctgctcgcca
    22681 cgcaaatcct gttattagtc gccattgcgg cgatgggttt tctcgaacca ggcacccaac
    22741 tccgctggat ggcggcgctg gcggtggtga tcgctttttg ctctgcctcc caggatattg
    22801 tcttcgatgc gtggaaaacc gatgtgctcc cggcagaaga acgtggtgcg ggcgcggcaa
    22861 tcagcgtgct gggttaccgt ttagggatgc tggtttccgg cggcctagcc ctgtggctgg
    22921 cagataaatg gttgggctgg cagggcatgt actggttgat ggcggcgctg ttgatcccct
    22981 gtattatcgc gacgttgctt gcaccagaac caaccgacac cattcctgtg cccaaaacgc
    23041 tggaacaagc ggttgttgca cctctgcgag atttctttgg tcgcaataat gcctggctta
    23101 ttttgcttct tatcgtgctg tataagctgg gcgacgcatt cgccatgagc ctgacaacca
    23161 cgtttttgat tcgcggcgtc gggtttgatg cgggtgaagt aggcgtggta aacaaaacgc
    23221 ttggcttact ggcgaccatt gttggcgcat tgtacggtgg gattttgatg cagcgcctgt
    23281 cgctgttccg ggcgctgctg attttcggca ttttacaagg tgcgtctaac gctggttact
    23341 ggctgctgtc gattactgat aagcatctct acagcatggg cgcagccgtc tttttcgaaa
    23401 acctctgtgg cgggatgggc acatcagcct ttgtcgcgct gttaatgacg ctgtgtaata
    23461 agtcattttc cgctactcaa tttgccctgc tctcagcgct ttccgctgta gggcgagttt
    23521 atgtcggccc cgtggcgggt tggtttgttg aagcacacgg ctggtcgaca ttctatctat
    23581 tctccgtcgc cgctgccgta ccggggctta ttttgctgct ggtttgccgc cagacgcttg
    23641 aatatacacg agtaaatgac aactttatct cccgtaccga atatccggca ggttatgcct
    23701 ttgccatgtg gacactggcg gcgggcgtca gcctgttggc cgtgtggtta cttctgttga
    23761 cgatggacgc gctggatttg acgcacttct ctttcctgcc tgctctgctg gaagtcgggg
    23821 ttttagtcgc cctttctggc gtcgtgcttg gtggtttgct ggattatctg gcgctacgaa
    23881 aaacgcatct gacgtaatct gtaaatatta tttagctgta gtaatcactc gccgtaatta
    23941 ttacggctaa ataaacatca gcatcgctta ttaatatttg ttcttttgtg cggcttagcg
    24001 tttggaaatt attgacgcca tttataaatt ctatttttct tacacgattc agctaatgag
    24061 tctttatttt ctcaccaccc agttgtcact ttaatgataa ttatttgtta aataattgtt
    24121 ttatttcgca ttggttatac caattgccca ccaagactcc ttctgcactc cccttttgtt
    24181 a
//
LOCUS       DADLTY010000050        23846 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.050, whole genome shotgun sequence.
ACCESSION   DADLTY010000050 DADLTY010000000
VERSION     DADLTY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.050"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..1095
                     /locus_tag="JN334_001680"
     CDS             <1..1095
                     /locus_tag="JN334_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY7437848.1"
                     /translation="VASPAQASVSDLTGDQPAAQPVPVSAPATSTAAVSAPANPSAEL
                     KIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNVEELLKSNTPLNVLALNQPENIENR
                     VNICYFALSPEDEARDAARHIRDQGKQAPLVLIPRSALGDRVANAFAQEWQKLGGGTV
                     LQQKFGSTSELRAGVNGGSGIALTGSPITPRATTDSGMTTNNPTLQTTPTDDQFTNNG
                     GRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATLYASSRSAQGTAGPDFRLEMEGLQY
                     SEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDAWSLANHFSQMRQVQGFEINGN
                     TGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            1053..1448
                     /locus_tag="JN334_001681"
     CDS             1053..1448
                     /locus_tag="JN334_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAY7437849.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            1468..2058
                     /gene="diaA"
                     /locus_tag="JN334_001682"
     CDS             1468..2058
                     /gene="diaA"
                     /locus_tag="JN334_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HAY7437850.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            2068..2643
                     /gene="yraP"
                     /locus_tag="JN334_001683"
     CDS             2068..2643
                     /gene="yraP"
                     /locus_tag="JN334_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HAY7437851.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(2757..3797)
                     /locus_tag="JN334_001684"
     CDS             complement(2757..3797)
                     /locus_tag="JN334_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY7437852.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(3870..4505)
                     /locus_tag="JN334_001685"
     CDS             complement(3870..4505)
                     /locus_tag="JN334_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAY7437853.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLDDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            4633..5151
                     /gene="yhbO"
                     /locus_tag="JN334_001686"
     CDS             4633..5151
                     /gene="yhbO"
                     /locus_tag="JN334_001686"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HAY7437854.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(5131..5574)
                     /locus_tag="JN334_001687"
     CDS             complement(5131..5574)
                     /locus_tag="JN334_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HAY7437855.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            5625..5927
                     /gene="yhbQ"
                     /locus_tag="JN334_001688"
     CDS             5625..5927
                     /gene="yhbQ"
                     /locus_tag="JN334_001688"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HAY7437856.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(5914..6417)
                     /locus_tag="JN334_001689"
     CDS             complement(5914..6417)
                     /locus_tag="JN334_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7437857.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(6411..6935)
                     /locus_tag="JN334_001690"
     CDS             complement(6411..6935)
                     /locus_tag="JN334_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HAY7437858.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            7144..8139
                     /locus_tag="JN334_001691"
     CDS             7144..8139
                     /locus_tag="JN334_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY7437859.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            8148..9026
                     /locus_tag="JN334_001692"
     CDS             8148..9026
                     /locus_tag="JN334_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY7437860.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            9107..10114
                     /locus_tag="JN334_001693"
     CDS             9107..10114
                     /locus_tag="JN334_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAY7437861.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(10232..11476)
                     /gene="mtr"
                     /locus_tag="JN334_001694"
     CDS             complement(10232..11476)
                     /gene="mtr"
                     /locus_tag="JN334_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAY7437862.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(11630..13519)
                     /locus_tag="JN334_001695"
     CDS             complement(11630..13519)
                     /locus_tag="JN334_001695"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HAY7437863.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(13512..13592)
                     /gene="yrbN"
                     /locus_tag="JN334_001696"
     CDS             complement(13512..13592)
                     /gene="yrbN"
                     /locus_tag="JN334_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HAY7437864.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(13699..14583)
                     /gene="nlpI"
                     /locus_tag="JN334_001697"
     CDS             complement(13699..14583)
                     /gene="nlpI"
                     /locus_tag="JN334_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HAY7437865.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(14692..16827)
                     /gene="pnp"
                     /locus_tag="JN334_001698"
     CDS             complement(14692..16827)
                     /gene="pnp"
                     /locus_tag="JN334_001698"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAY7437866.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(17074..17343)
                     /gene="rpsO"
                     /locus_tag="JN334_001699"
     CDS             complement(17074..17343)
                     /gene="rpsO"
                     /locus_tag="JN334_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAY7437867.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(17492..18436)
                     /gene="truB"
                     /locus_tag="JN334_001700"
     CDS             complement(17492..18436)
                     /gene="truB"
                     /locus_tag="JN334_001700"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAY7437868.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(18436..18837)
                     /gene="rbfA"
                     /locus_tag="JN334_001701"
     CDS             complement(18436..18837)
                     /gene="rbfA"
                     /locus_tag="JN334_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAY7437869.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(19001..21649)
                     /gene="infB"
                     /locus_tag="JN334_001702"
     CDS             complement(19001..21649)
                     /gene="infB"
                     /locus_tag="JN334_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY7437870.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(21674..23161)
                     /gene="nusA"
                     /locus_tag="JN334_001703"
     CDS             complement(21674..23161)
                     /gene="nusA"
                     /locus_tag="JN334_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAY7437871.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(23189..23641)
                     /gene="rimP"
                     /locus_tag="JN334_001704"
     CDS             complement(23189..23641)
                     /gene="rimP"
                     /locus_tag="JN334_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAY7437872.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
BASE COUNT         5629 a         6652 c         6000 g         5565 t
ORIGIN      
        1 gttgccagcc cggcacaagc ctcggttagc gatctgaccg gtgatcagcc tgcggcccag
       61 ccggtgcctg taagcgcccc ggcgacaagc accgcagcgg taagcgcacc cgcaaatcca
      121 tccgcagagc tgaaaatcta cgatacctca tcacaaccac ttagccagat cttaagccag
      181 gttcagcagg atggcgcgag tattgtggtt ggtccgttgc tgaaaaataa cgttgaagag
      241 ttgctgaaga gcaacacccc gctgaacgta ctggcactga accagccgga gaatatcgaa
      301 aaccgcgtca atatttgtta cttcgcgctt tcaccggaag acgaagcgcg cgatgcagcg
      361 cgtcatattc gtgaccaggg taaacaagcg ccgctggtgc tgatcccacg cagtgcattg
      421 ggcgatcgcg tagccaatgc gtttgcgcaa gagtggcaga aactgggcgg cggcaccgtt
      481 ctgcaacaaa aatttggttc caccagcgaa ttacgcgcgg gtgttaacgg cggttctggt
      541 attgctttaa cgggtagccc gattactccc agagcgacaa ccgactccgg catgacgacc
      601 aacaatccaa cgctgcaaac cacgccaacc gatgaccagt tcaccaataa tggcggtcgt
      661 gtcgatgcgg tctacattgt ggcaacgccg ggtgaaatcg cttttatcaa accgatgatc
      721 gccatgcgta acggtagcca gagcggtgca acgctgtacg ccagttcccg cagcgcacaa
      781 ggcaccgcag gcccggattt ccgactggag atggaaggct tgcagtacag cgaaatcccg
      841 atgctggcag gcggtaatct gccgttaatg cagcaggcgc tcagcgcggt gaacaacgat
      901 tattcgctgg ctcgcatgta tgcgatgggc gttgatgcct ggtcgttggc aaatcatttc
      961 tcacaaatgc gccaggttca gggttttgaa atcaacggta ataccggaag tctgacggct
     1021 aacccggatt gcgtgattaa caggaagtta tcatggctac agtaccaaca aggtcaggta
     1081 gtccccgcca gttaaccacc aaacagaccg gcgatgcgtg ggaagcacaa gcgcgtcgct
     1141 ggctggaagg caaaggactg cggtttatcg ccgctaacgt gaacgagcgt ggcggcgaga
     1201 tcgatctgat aatgcgtgaa ggcctgacca ccgtttttgt cgaggtacgc tatcgccgct
     1261 ctgcgcttta tggcggcgcg gcagccagtg tgacccgcag caaacaacac aaattattac
     1321 agactgcccg cttgtggctc gcgcgtcata atgggagttt tgatactgtg gattgccggt
     1381 tcgatgtggt agccttcacc gggaatgagg ttgagtggat taaggatgcc tttaatgacc
     1441 actcataatt aaggtttaag gattagcgtg caagaaagaa ttaaagcttg cttcactgaa
     1501 agcattcaaa ctcaaattgc ggcggcagag gcgcttccgg atgccatctc ccgtgcagcc
     1561 atgacgctgg ttcagtctct gctcaatggc aacaaaatcc tctgttgtgg taatggaact
     1621 tccgctgcca atgcacagca ttttgctgcc agcatgatca accgtttcga aacggagcgg
     1681 cccagcttac ctgccattgc actaaatact gataatgttg tcttaacggc gattgccaac
     1741 gatcgcttac atgatgaagt gtatgcaaaa caggtgcggg cgctgggtca tgcgggagat
     1801 gtattgttag ccatttccac ccgtggcaac agccgcgata ttgttaaagc agttgaagcc
     1861 gccgttacgc gtgatatgac cattgtggca ttgaccggct atgacggcgg cgaacttgca
     1921 ggtttgttag ggccacagga tgtggagatc cgcattcctt cgcatcgtag tgctcgcatt
     1981 caggaaatgc atatgctgac ggtaaattgc ctgtgcgatc tgatcgataa cacgcttttc
     2041 cctcaccagg atgattaagg agaatacatg aaggcattat cgccaatcgc agtccttatt
     2101 tccgcgctgc tgttgcaagg ttgtgttgcc gctgccgtag tgggtaccgc tgctgtgggt
     2161 accaaagccg caactgaccc acgcagtgtc ggcacccagg tggacgatgg taccctggaa
     2221 gtgcgcgtga acagcgcatt gtcgaaagac gaacagatta agaaagaaac ccgtattaat
     2281 gtcacggcct atcagggcaa agtgctgctg gttgggcagt caccaaatgc tgaactttcg
     2341 gctcgcgcca aacagattgc tatgggcgta gacggtgcca acgaagtgta taacgagatt
     2401 cgtcagggcc agccgattgg tctgggcgaa gcatctaacg atacgtggat caccaccaaa
     2461 gtgcgttcgc agctcttaac cagcgacctg gtgaaatcgt ccaacgtgaa agtgaccacc
     2521 gaaaacggtg aagtgttcct gatggggctg gtgactgaac gtgaagcgaa agcggcggca
     2581 gatattgcca gccgggtgag cggcgtaaag cgggttacca cagcgtttac gtttattaaa
     2641 tagcagcccc ttgtaatgcc tgatgcgacg cttgccgcgt cttatcaggc ctacaacgat
     2701 gcaaaccgta ggtcggataa ggcgtttacg ccgcatccga catgttttcc ctcaaatcag
     2761 aacaacagcg ccagcccgcc gacaatcaca ccagacactg ccaccatcgc ccctgtcagc
     2821 cataaggctt tcgccgggaa cgctttgcgc agcataatca gtgacggcaa actcactgcc
     2881 gggagcgtca tcaacaatgc cagcgccgga gcggttccca tacctgccag catcatcgtt
     2941 tgtacaatcg gaatttctgc tgccgtggga atgacaaaca agcatcctgc taccgccatc
     3001 gccaccaccc acatcaggct gttatcgaca gcaccatcgg catgggggaa taaccagacg
     3061 cgtgcggcac ccaacaccag tactgcaagg atgtaaaccg ggatcgtact ccagaaaagt
     3121 gtccatagcg ccctgcccca gcggctaaaa aaaccgctct gtgcttccgg tatgtcaatt
     3181 tcgaccggtg cctgcgtttg cggtgtttca cgcacccatt tttgcaccag cgtcgcaatc
     3241 agcaacacca tcaccagccc ggccaccaga cgaatcgccg caaaacccca gccgaggaca
     3301 aaccccataa acaccagcgt cgccgggttt aacaccggat tgcccatcca gaatgccagc
     3361 gcaccgccca tcgatacctg ttggcgacgc attcccgccg cgaccggagc cgcacagcag
     3421 gtacacatca tgcccggcag cgaaaacagc gttcccagca gcgtgccgcg aaagcgcgat
     3481 tgcccaagcg tacgcaacaa ccagtcacgc gggatcagca cctgaatcaa cgaaccgagg
     3541 atcaccccca gcaccgccgc tttccatact gcgaggaaat agatcatcgc gtaatccaac
     3601 gccgcttgcc acggattagc atccgcctgc gcaaggatag atttaccgat actgtgggtt
     3661 tcggctgcag taaacgcttt gccgtagtaa ggttgccatt tcacatacca gagaccggca
     3721 atgacaacga gaaagaaaag cgcgggtttc caccactgaa tgggcgttgc cgcctgagat
     3781 gaagactgac cagtcatagc attccccagg aataagttat gataattgag cgcgtgaata
     3841 ttacgctcac tatcaattct tgggaataat tattcagctc ttttgcgtaa ttctgaagag
     3901 cttaaaatcg tcacgccttc atgctcaggc cgcatcgcct ctgccagcat gacccgcgcc
     3961 acgtctctcg catcaatgga tttccagtta cctggtaaca aacggaacag cggcgcaaaa
     4021 agcgtttcgt tcatccgctg tttgctacga tcgtccagta acatcgacgg gcgagcaatg
     4081 gtcaatttcg gccagttctg ggcaattaat gcttcttcca tctccccttt gacgcggtta
     4141 tagaaaaacg gcgagtgggc attggcaccc atagcactga ccaccagcat atgctgcgcg
     4201 cccagtcgcc gcccggttaa tgcggtatcc actaccagcg tgtaatcggc atgaataaac
     4261 gcctctttgc tccccgcttc tcgccgcgtg gtgccgagac aacaaaacac aatgtcgatg
     4321 ggttcggtga cctgcgccaa cgcgtcactc agttgcggat catggggatt aaacaccccg
     4381 ggcatatcgc ccaacggacg tcgcgtcggc gcagcaatgg cgttaacttt cggttcatta
     4441 atcaacatcc gcagcaggtg accgcccacc agccccgttg cgcctgtaat cagtacctga
     4501 ctcatcttcg ttcctttaca gaattgtctg ccttgcgctc cccggctcat gcaccagtct
     4561 taatagacca tgaggtaatt atccccgatt gtggaaaatt cgcctcatcc aatgcaacaa
     4621 cgcggaggaa gcatgagtaa gaaaattgcc gttttaatca ctgatgaatt tgaagattca
     4681 gaatttactt cacccgcaga cgagttccgt aaagccggac acgaagtgat tactattgaa
     4741 aaacaagcgg gtaaaacggt gaaaggcaaa aaaggagaag ccagcgtgac catcgataaa
     4801 tccatcgatg aagtgacgcc agcggagttt gatgccctgt tgctaccggg tggtcattca
     4861 ccggattatc tgcgtggcga caaccgtttt gtcaccttta cccgtgattt tgtgaatagc
     4921 ggcaaacctg ttttcgccat ctgtcacggt ccgcagctgc tgatcagcgc cgatgtgatt
     4981 cgggggcgca aactgaccgc agttaaaccg atcattattg atgttaaaaa tgcgggcgtg
     5041 gaattttacg atcaggaagt cgtggttgat aaagatcagc tggttaccag ccggacaccg
     5101 gacgatctgc cagcgtttaa ccgcgaggcg ttacgcctgc tcggtgcctg agtcgcgtag
     5161 ccaaatcatt tttttaccaa agcccagcgt gttgtcggtg aatttgattt catcgagccg
     5221 gatttcccac accggggccg acagcattct ggcaaccgga aagcgacgat tgtacgcctt
     5281 gcgcgcgagg tcgctttcct caccttccag cctgcggatc tcacctttaa actgcacacc
     5341 gcgaattaac gctaccgttt tcggctgacc gtttaccgtt ccggcaaccg cagcctgcgg
     5401 cccactcatc tgcgcgtggc gcgttttttc ttccgtcaaa atgtagaagg cgactttctg
     5461 cgcatcaaaa agataaaagg cattagcgca ccagagttcc ccttcctgct gcacacacca
     5521 ggtgacaaca tgttgttttg ccagccaacg gctgatggcg atgagtgttt ccattgctgt
     5581 tctcttttat actgtgggcc tgaccttaac atgcgatgca gacgatgaca ccctggtttc
     5641 tttacttgat ccgtaccgcc gacaataagc tttataccgg gatcaccacg gatgtcgaac
     5701 gccgctatca gcagcaccaa agcggcaaag gggcgaaagc actgcgcggg aaaggagaac
     5761 tgacgctggc gttttctgcg ccagtgggcg accgttcgct ggcactacgg gcggaatatc
     5821 gcgttaagca actgaccaaa cggcaaaaag agcgtctggt agcggaaggc gcagggtttg
     5881 cagaactgtt gagcagcctg caaaccccgg agattaaaag cgattgaaat gctcgtgata
     5941 ctcaaccaga ccggttacgc cattcagcgc gtcatccgct aaacgatgta ccaggaaggc
     6001 gctttcagtg cccggccagc ggcaacgcag atcgtgatgc gccgccagtt caaagccgaa
     6061 acgactgtac agcgccggat cgcccagcgt caccactgcg gcatagccga actcattaag
     6121 cgaatcgagt ccttcataga ccagttggcg tgccagcccc tgtccacggt atttttcatc
     6181 aaccgccaac ggtgccatgc cgacccattg caggtcttcg ccctgcacat caaccggact
     6241 aaatgccaca tagccaatga cctgaccttc gtcatctgtc gccaccagcc ccagcgtcag
     6301 aaagccatct tcacgcagat cgtgaaccag cttcgcttcc gcatcacttt cgaatgagcg
     6361 acgcagcagg gcatcaatac ccggcgcatc aatgggaatt tctactcgaa ttagcatggt
     6421 tcacctaccg atgtctgttt ggtttcaggc gcggttttca ttcccgcctc aacaaaatcc
     6481 gccagttgca gcagcatcat gcgtaaggct ttcggcattt gctccagttc aatggcgtcc
     6541 atcaggtttt tcacatacac ccccagctcc gtatcgcctt caatcaccag ccgacgctgg
     6601 aagaagagcg tatccggatc ttgtttacgc gccgcaatca tcagcagatc gctggcgtcg
     6661 gcactaaaac tcacatcagc ttgcgtgttc tggctgacca ccagtttgcc attcaccacc
     6721 gaggtaaacc attgcaggtc aatatcacgc acatgaatac ttaaccagcg gccttcaaga
     6781 aactccagct cgccatcatc cagcgcctga cggaattgcc agcttaagac ctgctcaaga
     6841 acctggcgtt ttagcgcaaa tggcgtcagt tttaccggta cactcaacag agatggcccc
     6901 aaatgcacaa tacgggaacg cagtttatcc aacacgagtt ttactccctg tttcaacaat
     6961 catcctattt tgccatatca gaaaaataac atagcggtat aaatcaacaa ttccatatga
     7021 aattgctgct accaccaata caactttaac tgccttaaat caaaaattgt cgcagcaagg
     7081 ttaactaaaa tcccagttcg ttaacatttt tgcgttttga tagcgcaacc ttcaggaaaa
     7141 attatggagc tgctctgccc tgccggaaat ctcccggcgc ttaaggcggc catcgaaaac
     7201 ggcgcagatg ctgtttatat cgggctaaaa gatgatacca atgcccgtca cttcgccggc
     7261 cttaacttta ccgagaaaaa attgcaggaa gcggtgagtt ttgtccatca acatcgccgc
     7321 aaacttcaca tcgcgattaa cacttttgcg catccggacg gttacgcccg ttggcagcgt
     7381 gccgtggata tggcggcgca gctgggtgcc gacgcgctga tcctcgccga cctcgccatg
     7441 ctggagtacg ccgccgagcg ttacccgcat attgaacgtc atgtgtcagt gcaggcttcg
     7501 gcgaccaatg aagaggcgat taacttttat catcgccatt ttgacgttgc ccgcgtggtg
     7561 ctgccgcgcg tgttgtcgat tcatcaggtg aaacagctgg cacgggttac tcctgtcccg
     7621 ctggaagtgt ttgctttcgg cagcctgtgt attatgtcgg aaggccgttg ctatctgtcg
     7681 tcgtatctga cgggtgagtc gcccaacact gtgggtgcgt gttctccggc ccgtttcgtg
     7741 cgctggcaac aaacgccgca ggggctggaa tcccgcctga acgaagtgct gatcgaccgt
     7801 tatcaggacg gcgaaaacgc aggttatccg acgctgtgta aagggcgtta tctggtggac
     7861 ggcgagcgct atcacgcgct ggaagaacca accagtctca ataccctgga actgctgccg
     7921 gagttaatgg ctgcgaatat tgcttcggtg aaaattgaag gccgccagcg tagcccggcg
     7981 tatgtcagcc aggcggcgaa agtctggcgt caggctatcg accgttgtaa ggccgatccg
     8041 caaaacttcg taccgcaaag cgcgtggatg gagacgctcg ggtcgatgtc cgaaggcacg
     8101 caaaccaccc ttggcgcgta tcaccgtaaa tggcagtgag aagagcaatg aaatattcct
     8161 tagggccagt gctgtggtac tggccaaaag agacgctgga agatttttat cagcaagccg
     8221 ccgccagcag cgccgacgtg atttatcttg gtgaagcggt atgcagcaag cgccgggcaa
     8281 ccaaagttgg cgactgtctg gagatggcaa aatcgctcgc cggaagtggc aagcagattg
     8341 tgctctccac gctggcgctg gtgcaggcat cctctgaact gggcgaactg aaacgctatg
     8401 ttgagaacgg tgaatttctg attgaagcca gcgatctcgg cgtggtgaat atgtgcgctg
     8461 aacgcaaact gccgttcgtc gccgggcacg cgctgaactg ctacaacgcg gtgacgctga
     8521 aaatattgct caaacagggc atgatgcgct ggtgtatgcc ggtggagctt tcccgcgact
     8581 ggctggtgaa tctgcttaat cagtgcgatg agctgggcat tcgcaaccag tttgaagtgg
     8641 aagtcctgag ctacggtcat ctaccgctgg cctactccgc ccgctgcttt accgcgcgtt
     8701 cggaagaccg cccgaaagat gagtgtgaaa cctgctgcat taagtatccg aacgggcgca
     8761 acgtgctgtc gcaggaaaac caacaagtgt ttgtactcaa tggcattcag accatgagcg
     8821 gctacgttta caacctcggt aacgagctgg catccatgca gggattggtt gatgtagtcc
     8881 gcctgtcacc gcagggcact gacactttcg cgatgctcga cgccttccgc gctaatgaaa
     8941 atggcgctgt gccactgccg ttgacggcaa acagcgactg taacggctac tggcggcggc
     9001 tggcaggact ggagctgcaa gcctaagtaa atagctcact ttgttaacaa ctttaactat
     9061 tctttaatgc agtattaaag attaatcggt aacaaagtga gctgttatga ctgataaaac
     9121 cattgcgttt tcgctactcg atctggcccc catacccgaa ggctcttcag cgcgggaagc
     9181 gttctcccac tctctcgatc tcgcccgtct ggctgaaaag cgcggctatc atcgctactg
     9241 gctggcagaa caccacaata tgaccggcat tgccagtgct gcaacatcgg tattgattgg
     9301 ctatctggcg gcgaatacca ccacgctgca tctggggtct ggcggcgtga tgttgcctaa
     9361 ccacttaccg ttggtcattg ccgaacagtt cggcacactc aatacgctct atccggggcg
     9421 aatcgatttg gggctgggtc gtgctccggg tagtgaccaa cggacaatga tggcgctacg
     9481 tcgtcatatg agcggcgata ttgataattt cccccgcgat gtcgcggagc tggtggactg
     9541 gtttgacgcc cgcgatccca atccgcatgt gcgcccggta ccaggctatg gcgagaaaat
     9601 ccccgtgtgg ttgttaggct ccagccttta cagtgcacaa ctggcggcgc agcttggtct
     9661 gccatttgcg tttgcctcac acttcgcgcc ggatatgttg ttccaggcgc tgcatcttta
     9721 tcgcagtaac ttcaaaccgt cggctcggct ggaaaaacca tacgcgatgg tgtgcatcaa
     9781 tattatcgcc gccgacagca accgcgacgc tgaatttctg tttacctcaa tgcagcaagc
     9841 ctttgtgaag ctgcgccgtg gcgaaaccgg gcaactgccg ccgccgattc aaaatatgga
     9901 tcagttctgg tcaccgtctg agcagtatga cgtgcagcag gcgctgagta tgtcgttggt
     9961 aggcgataaa gcgaaagtgc gtcatggttt gcagtcgatc ctgcgcgaaa ccgacgctga
    10021 tgagattatg gttaacgggc agattttcga ccaccaggcg cgcctgcatt cgtttgagct
    10081 ggcgatggat gttaaggaag agttgttggg atagtgtgtc ttaacgcggg aagccttatc
    10141 cgagctggca acgctgtcct acgtaggcct gataagcgaa gcgcatcagg cattgtgtag
    10201 gcagcagaaa tgtcggataa ggcaccgctg attactgata caccggcagt aaattaaagc
    10261 tcgataaaat atgcaccagt gcgttgccga cgccaaacac cagaatcagc gcaatcatcg
    10321 gcttgccacc ccagacgcgg aatttcgggc tgccaaagcg tttacgcgat gcgcgggcca
    10381 gcagtgccgg aacaattgcc gcccagatgg tagccgctaa accagcataa ccaatggcat
    10441 acaggaatcc gttcgggaac agcagaccac ccacaacagg cggggcaaag gtcagcaatg
    10501 ccgttttcaa gcggcccaca gccgagtcgt cgaaaccaaa cagatctgcc agatagtcaa
    10561 acaaacccag cgttacgcca aggaacgaac tcgctaccgc aaagtttgag aacacgacca
    10621 gcagaaggtc caggctacgg ctgttcagca cgccgcttaa tgcttgtacc aacacatcaa
    10681 tattaccgcc cttctcggcg atgccgataa actccggacg agggatgttg cccattgtcg
    10741 ccagcaacca gatggtatac agcgccagtg ccatcagcgt accgtacacc aggcatttca
    10801 cgatggtttt cggatctttg ccgtaatact tcatcaggct tggcacgtta ccgtgataac
    10861 caaacgacgc caggcagaac ggcagggtca tcagcaaata cggcgcataa gatgcattgc
    10921 tttcggcaac gttgaacaat gtcgcaggct gcacatgccc cagcaggcta ccgaaggtga
    10981 ggaagaaggt aatgactttc gcccccagca caatcgccgt catgcggctg acggctttag
    11041 tactcaacca caccacaaac gctaccagca gtgcaaaacc aaaacccgcc gcccgtgccg
    11101 ggacgtttag cgacatctct gcgaaggtgt gatgcagaat cgaaccactg gcagaaatat
    11161 aggcataggt caggatatag agcacaaagg caatggaaat gccgttgacc acgttccagc
    11221 ctttgcccag caaatctttg gtgatggtgt caaaactcga accgattcgg taattcaggt
    11281 tagcttccag aatcatcaag ccggaatgca gcatacagaa ccaagtaaag atcagcgctg
    11341 ccattgacca gaaaaaccac gccccggaca tgaccactgg cagagaaaac atccctgcgc
    11401 caataatggt gccgccgata atcaccacgc cgccaagcag cgacggtgac gtttgggtgg
    11461 tggttagtgt tgccatgagg gcttctctcc agtgaaaaat agtgcgactg cgttgttatg
    11521 cattgcactg taccagtaca tgagtacaaa agacagaaaa aaagccccga tggttaaaat
    11581 cggggctgta tatattattt tacagattgt gttcgctgtt cagcgatgat tacgcatcac
    11641 caccgaaacg acgacgaccg gtagagtcat cacgacgcgg tgcgcggcct tcacgacgtt
    11701 cgccgctaaa acgacgacca tcaccacgac caccttcacg gcgttcaccg ctgaagttac
    11761 gaccgccttc acgacgctcg ccaccgaaac cacgaccacc gccacgacgc tcaccgccag
    11821 tatgcggctg tgcatcgccc agtaactgca tgttcatcgg cttgttgaga atgcgagtgc
    11881 gcgtaaagtg ttgcagcact tcacccggca tacctttcgg cagttcgatg gtggagtgag
    11941 aagcaaacag cttgatgtta ccgatgtaac ggctgctgat gtcgccttcg ttagcaatcg
    12001 cgccaacgat atgacgaact tcaacaccat catcgcggcc cacttcaatg cggtacagct
    12061 gcatatcgcc aacatcacga cgttcacgac gcggacgatc ttcacggtcg ccacgcgggc
    12121 cacggtcgtt acgatcgcgc ggaccacggt catcacggtc acggaattca cgtttcggac
    12181 gcatcggcgc atctggcgga acaatcagag tacgttcacc ctgtgccatt ttcagcagtg
    12241 ccgcagccag agtttcgaga tccagctctt caccttctgc agtcggctga attttgctca
    12301 gcagtgcgcg gtattgatcc agatcgctgc tttccagctg ctgctgtact ttagcggcga
    12361 atttttccag acggcgtttg cccagcagtt ctgcgttcgg cagttctact tccggaatag
    12421 tcagcttcat agtacgttca atgttgcgca gcagacgacg ctcgcggttc tcaacgaaca
    12481 gcagcgcgcg gccagcacga cccgcacgac cggtacgacc gatacggtga acgtaagact
    12541 cagaatccat cgggatatcg tagttaacca ccaggctgat acgctcaacg tccaggccac
    12601 gggccgcaac gtcggtcgca atcaggatgt ccagacgacc gtctttaagg cgttccagtg
    12661 tctgctcacg cagcgcctgg ttcatgtcac cgttcagcgc ggcgctattg tagccgttac
    12721 gctcaagagc ttcagccact tccagagtcg cgtttttggt acgaacgaag ataatcgccg
    12781 catcaaaatc ttccgcttcc aggaaacgta ccagtgcttc gtttttacgc ataccccaca
    12841 cagtccagta gctctggctg atgtcaggac gggtagtcac gctggactga atacgcactt
    12901 cctgcggctc tttcataaag cggcgggtaa tgcgacgaat cgcttccggc atggttgcag
    12961 agaacagagc ggtctgatga ccttccggga tctgcgccat aatggtttca acgtcttcga
    13021 tgaagcccat gcgcagcatt tcgtcagctt catccagaac cagaccgctc agtttagaga
    13081 ggtccagagt gccacgtttc aggtggtcca gcagacggcc cggagtaccg acaacgatct
    13141 gcggcccctg acgcagggcg cgtaattgca cgtcataacg ctggccgccg tacagggcaa
    13201 ccacatttac gccgcgcatg tgtttagaga aatccgtcat tgcttcagca acctgtaccg
    13261 ccagttcgcg ggtcggtgcc agcaccagaa tctgtggtgc tttcagctca ggatcaagat
    13321 tctgcagcag aggtaaagag aatgctgcag tttttccgct ccccgtctgg gccataccca
    13381 gaacgtcgcg gccattcagc agatgtggaa tacactctgc ctgaattgga gatggttttt
    13441 cgtaacccag atcgttaagg gcttcaagga taggagcctt caggcccaga tctgcaaaag
    13501 tggtttcgaa ttcagccatg tagtacgtgt gcctcaaaat taatggcggc cagtctacat
    13561 aactcatcat gaaattgatc agcaattttc attgaaaagt gtgaaccggc tcaaagtagg
    13621 tgtattaacg aacaacaacg ccctcacccg ttaaggtgat ggcaatcaaa aaagattacg
    13681 ggctgatgtg tacgtcagct attgctggtc cgattctgcc aggtcatctt ggtcctggcc
    13741 caggagcgat aattccaaca atgcgtatcg gtgctcaaca aagttatgaa cgttgttggc
    13801 aaccgccagt ttgaacagtg ccgtggcgct gtccaaatcc cccagactta ggtagtactt
    13861 acctaaatag aagttggttt cactgagatg ctcagcgagc gaggtgttat ccgttgcgtc
    13921 cgccttgagc ctttccatta acgtttgttc gctaatgttg cccaggtaga actcgacaat
    13981 gttccatccc cactgttcct tatccgattt ttcgaagtgc tgtttcaata cttctttagc
    14041 ctgcttctca tcgagcttct gctcggcgag ataaagccac agactacgga aaggatcatt
    14101 gggatcgtct tgataaaacg ccagcagatc atcttgcgct aacttgtcac gaccgccgta
    14161 atataatgca atcccgcgat tcaagtgcgc gtagttgtaa gttggatcaa gctcaagtac
    14221 agaatcaaac gcttcatagg cagcatcaaa attgcctgcc tgcgttaaat atatgcctaa
    14281 gtaattgaat acttcaggca tatccggtcg gattgccagc gcttgcgaaa aatcgttacg
    14341 cgctaatgcc ctcagaccga gactatcata caacactccg cgctcatata aaagctgtgc
    14401 gcgttcgtca tcggttaaag cccgactggc aaggatttgt tccatacgtg ccagaatcac
    14461 ttcctgctgt aaagtcggtt gcaatggtac cgcgaggact tcacttttac gccaggaagt
    14521 attactgcat cctgcaagcg taagtgctgt cgcaacgaaa caccagcgca aaaaaggctt
    14581 catttcccac tcccgaagac cacggttgaa tgaacgtcct gttcccggtt gctaacaagg
    14641 cgtcctgccc ggttaaaagc cccccgccgc agcggagggc aaatggcaac cttactcgcc
    14701 ctgttcagca gccggagctt ccggtgctgc agcaggttga gactgctcag tcgcttcttt
    14761 aatgctcaga cggatacggc cctggcgatc aacttccaga actttcaccg gtacttcctg
    14821 acccatctgc aggtaatcgg tcactttctc aacgcgtttg tcagcgattt gagagatgtg
    14881 taccagacct tctttaccgc cgccgatggc aacaaatgcg ccaaagtcaa cgatacgggt
    14941 cactttacca gtgtagacgc ggcccacttc gatttctgca gtgatctctt cgatacgacg
    15001 aatagcatgt ttcgctttct cgccgtcggt cgctgcaatc ttcacagtac cgtcatcttc
    15061 gatttcgatg gtggtgccag tttcttcggt cagggcacgg ataacagagc cgcctttacc
    15121 gataacgtct ttgatcttgt ccgggttgat cttgatggta tggatacgtg gtgcgaactc
    15181 agagatatcg ccacgcggcg cgttgatcgc ctgttccatt acgcccagga tatgcagacg
    15241 cgcaccttta gcctggttca gcgcaacctg catgatctct ttggtgatac cttcaatttt
    15301 gatatccatc tgcaatgcag agataccctc gcgggaaccc gcaactttga agtccatatc
    15361 gcccaggtga tcttcgtcgc ccaaaatgtc agacagtaca acgtagttgt cgccttcttt
    15421 caccagaccc attgcgatac ccgcaacggc agctttgatc ggcacacctg cgtccatcag
    15481 cgccagagac gcgccgcaca cggaagccat agaagaggaa ccgttggatt cagtgatttc
    15541 agacacaaca cgtacggtgt acgggaattt gtccatatcc ggcatgactg ccagcacgcc
    15601 gcgcttcgcc agacgaccgt gaccaatttc acgacgcttc ggagaaccga ccatgccggt
    15661 ttcgcctacg gagtacggag ggaagttgta gtggaacagg aaggtatcgg tacgttcgcc
    15721 catcagttca tcaagaacct gcgcgtcacg agcagtaccc agcgttgcgg taaccagcgc
    15781 ctgcgtttca ccacgggtga acagcgcaga accgtgagta cgcggcagca cgccagtacg
    15841 cacatccaga ccacggatca tatctttttc acgaccgtcg atacgcggtt cgcctgccag
    15901 tacgcggcta cgaacaacgt ttttctcgat cgcgtgcaga atttcaccca gttcgttttc
    15961 gtccagggtt tcgtcttcag caagcagcgt cgcgatggtt tcagatttga tgacatcaac
    16021 ctgcgcataa cgctcttgtt tgtcggtgat gcggtaagca tcgctcaggc gagcttcagc
    16081 cagtgcagca acgcgcgcgt tcagcgcttc gtttaccggc tccggctgcc agtcccaacg
    16141 tggtttaccg gcttctttca ccagttcatt gatgttctga ataacaacct gctgttgttc
    16201 atgaccgaac actactgcgc ccagcatctg gtcttcgctc agcagttcag cttcagattc
    16261 aaccatcagt acagcggctt cagtaccggc aacaaccaga tccagtttgc tctctttcaa
    16321 ctcgtcctga gtcgggttca gtacgtactg gtcattgatg taacctacgc gagcagcacc
    16381 aatcggacca ttgaacggaa taccagacag agacagtgct gcggaagcac caatcatcgc
    16441 gacgatatcc gggttaactt gcgggttaac agaaaccacg gtggcgataa cctgaacttc
    16501 gttgacgaag ccttccggga acagcggccg aatcgggcgg tcaatcagac gcgcgatcag
    16561 ggtttcgcct tcgcttgggc ggccttcacg acggaagaag ctacccggga tacgaccagc
    16621 agcgtaggta cgctcctgat agttaacggt cagtgggaag aagtcctgac ctggtttggc
    16681 ttttttctgg ccaacaacgg taacaaatac cgcggtgtca tccatgctaa ccataacagc
    16741 ggcagtagcc tgacgagcca tcatgccggt ttccagagtc acggtgtgtt ggccgtactg
    16801 gaatttacga acgatcggat taagcaatgt aatatccttt ctctttctta gacagtacct
    16861 tacggcactg gtgttaatac ccgatcttct gcgcatcctc gcgactaatg acaaccctaa
    16921 cccagctcta tgtgggtaaa gcctctcatt agccgcgcga acctctgcaa cggaagatca
    16981 ttcatagcaa caatacatta gtttccagtg aattgctgcc gtcagcttga aaaaaggggc
    17041 cactcaggcc cccttttctg aaactcgcaa gaattagcga cgcagaccca ggcgctcgat
    17101 gagctgggtg taacgtgcta cgtctttacg tttcaggtag tcgagcagtt tacgacgctg
    17161 agaaaccatg cgcagcagac cacgacggct gtggtgatct tttttgtgct ctgcaaagtg
    17221 gccctgcagg tggttgatct gtgcagtcag cagtgctacc tgaacttcag tagaaccggt
    17281 gtcgtttgcg tcacgaccaa actcagaaac gattttagct gttgcttcag tacttagaga
    17341 cattttaaaa ctccaaagta tatagaatga aaggacgccg atctctaatt cagcgatccc
    17401 agtgtacgtt acgcaaagtg ttaaacaatt tacgcgacgt taagcggcag tattctactc
    17461 gtagcgacct gttatcgcaa gacggttaac attacgccgg gtattcaacc accaggcgac
    17521 gaggcgcaac gcggccttca tcgtcaattt cgcccatacc gataaatttg ccgttctcac
    17581 cttctgtaac gcgaaccagt ccttccagcg gcgcaccgga tgtacgaact gggttaccat
    17641 ttttgaagta aacagaagac gttaacggaa gattcaccac cgggtagtcc gaagctggac
    17701 tgtccattgg catcagtaat ggatcaagta actccgcagc cggaatatcc tgctgttcgg
    17761 cttgttcgac cagttcacgc agatgttcca gggtcacaat ccgttcaacc ggatatttac
    17821 ttaccgccag acgacgcagg taaataacat gcgcgccaca accgagtttt tcacccaggt
    17881 catcaatgat ggtgcggata taagtgcctt ttgagcagtg aatttccagc tccagctcat
    17941 tgccttcgtg gcgaataaac agcaattcat aaacggtaat cgggcgcgct tcacgcggta
    18001 cttcaatgcc ctgacgcgca tattcgtaca gttttttacc ctgatatttc agtgctgaat
    18061 acatcgaagg gatctgttcg atatcgccac ggaaagtatc cagcgccgcc gccagttgct
    18121 ctgcgctaaa ggttaccgga cgttcttcaa cgatttgtcc gtcggcatca gaagtatcgg
    18181 tacgctgtcc aagacgcgca atgacccgat agcgtttgtc ggagtccagc agatactggg
    18241 aaaacttcgt cgcttccccg aggcaaattg gcaacatgcc agttgccagc gggtccagcg
    18301 caccggtatg cccggcacgg ttggcgttat aaatccgttt cactttttgc agcgcatcat
    18361 tgctggacat accctgcggt ttatccagca acaaaacgcc gttaatgtcg cgaccgcgac
    18421 gacgaggacg actcattagt cctccttgct gtcgtccggg ttaacacgac gttcttcgtc
    18481 atgtttgacc acgctggtca ccaggtttga catgcgcatc ccttcaacca gagagttgtc
    18541 gtagaagaag gtcagttccg gcacgatacg caggcgcatc gctttcccca gcaggctgcg
    18601 gatgaaacca gaagcttctt gcaacgcttt gatgcccgct ttaaccgcgt cttcatcttt
    18661 gtcgttgagg aacgtcacat atactttggc atacgccagg tcgcgagaca tttcgacacc
    18721 ggaaacggtg gtcatcatgc ccaggcgagg atctttaatt tcacgctgca ggatgagagc
    18781 gatctctttt tgcatttcct gcgctacgcg ctgcgggcga ccaaattctt tcgccataat
    18841 aaattctcct gacaaaaaag gggctgttag ccccttttta aaattaattt caggtggaag
    18901 ggctgttcac gttggcctga taagacgcgc cagcgtcaca tcaggcaatc catgccggat
    18961 gcggcgtaaa cgccttatcc cgcatggaac cctaaaaacc ttaagcgatg gtacgttgga
    19021 tctcgatgat ttcgaatact tcgatcacat cgccagtgcg gacgtcgttg tagttcttaa
    19081 cgccgatacc acattccata ccgttacgga cttcgttaac gtcatctttg aagcggcgca
    19141 gggactccag ctcgccttcg tagataacca cgttgtcacg cagaacgcgg atcgggttgt
    19201 gacgtttaac cacaccttcg gtaaccatac agcctgcgat ggcaccaaat ttcggcgatt
    19261 tgaacacgtc acgaacttcc gccagaccga taatctgctg tttcagttcc ggagacagca
    19321 taccgctcat cgccgctttc acttcgtcaa tcaggttata gatgacggag tagtaacgca
    19381 gatccaggct ttccgcttca atcactttac gtgcagaggc atcagcacgt acgttgaagc
    19441 caaccaggat ggcgttggac gccgcagcca gggtggcgtc ggtttcggtg ataccaccta
    19501 cgccagaacc gatgatcttc actttaactt cgtcagtaga cagtttcagc aaggagtcgg
    19561 agatcgcttc cacagaaccc tgtacgtctg ccttcaggac gatgttcact tcgtgaactt
    19621 cgccttcggt catgttggcg aacatgttct cgagtttaga tttctgctga cgcgccagtt
    19681 taacttcgcg gaatttaccc tgacgataca gtgcaacttc acgcgctttc ttctcgtcac
    19741 gtacaacggt aacttcatca cccgcagccg gtacgccgga caggccgagg atttccaccg
    19801 gaatggacgg acccgcttcc agcacttcct gacccagttc gttacgcatc gcacgaacac
    19861 gaccgtattc gaagccacac agaacgatat cgcccttgtg cagagtacct tcacgtacca
    19921 gaacggtagc aaccggacca cgacctttat cgaggaagga ttcgataacc gcaccgctcg
    19981 ccataccttt acgtaccgct ttcagctcca gaacttccgc ctgcagcagg atagcgtcca
    20041 gcagttcatc gataccagta cccgctttcg cagacacatg tacgaactgg ctttcaccgc
    20101 cccactcttc cggcaggatg ccgtactggg agagttcgtt cttaacgcga tccggatcgg
    20161 cttctggttt atcgatcttg ttcactgcaa cgaccaccgg aacctgcgcc gctttcgcgt
    20221 gctggattgc ttcgatcgtc tgcggcatta cgccgtcgtc ggcagcaaca accaggacta
    20281 cgatgtccgt tgcctgcgca ccacgagcac gcattgaagt aaacgcggcg tgccccgggg
    20341 tgtccaggaa ggtgatcatg ccgttttcag tttcaacgtg gtatgcacca atgtgctggg
    20401 taatgccgcc cgcttcgcca gaggccactt tcgttgaacg aatgtagtcc agcagagagg
    20461 ttttaccgtg gtcaacgtga cccatgatgg tcacaaccgg cgcgcgcggt tcagccgcag
    20521 cacccgtgtc acggtcgctc attaccgcct cttccagctc gttttcacga cgcaggataa
    20581 ctttatggcc catctcttca gcaaccagct gtgcggtttc ctgatcgata acctggttga
    20641 tggttgccat tgcgcccagt ttcatcatcg ctttgatgac ctgagagcct ttaaccgcca
    20701 tcttgttcgc cagttcgcca acggtgatag tttcgccgat cacaacgtca cggttaacgg
    20761 cctgagcagg cttctggaag ccttgctgca gcgaagaacc tttacgtttt ccgcctttac
    20821 cgccacgtac tgctgcgcgt gcttcttcac gatcagcttt tgattcagcg tgtttgttgc
    20881 ctttcttcgg acgcgctgct ttcgcgttac gaccacggcc acggccgcct tcgacttcac
    20941 gatcgctttc gtcttctgcc tggcgagcat gttgagaagt agtgacgtga taatcgctgg
    21001 aatcttcagt cggttccgcg ttatcagtcc atttgttttc ttccgccata cgacgtgctt
    21061 cttcagcaac gcgacgtgct tcttcttcga gtttacgacg cgcttcttct tcagctttac
    21121 gcttgagctc tgcagcttcc tgctcacggc gggctttttc agcctgggcg tttttagtca
    21181 tatcgtcttg ttgattgctc actttgtctt tttccgcagc ttcacgtttc gcttgttcag
    21241 cagcttcacg cttagcttgt tctgcggcct cacgttcagc tttttgttgc gcctcgcgtt
    21301 tagccgattc ttctgcctca cgacgggctt gctcttccgc tgcaaggcgt tcagcctctt
    21361 gcggatcgcg tttcacaaag gtgcgtttct tgcggacttc gatttgtacc gatttgcttt
    21421 ttccaccggt accaggaatg ttaagggtgc tgcgtgtttt acgttgcagc gtcaatttgt
    21481 ccgggcctga atttttctga ttcaggtggt caatcaaagt ctgtttctct tgtgcagaca
    21541 cagagtcgtc agcagacttc cggatacctg catcagcaaa ttgctgtacc aggcgttcca
    21601 cggaggtctg tcgctctgcg gccagcgttt taatcgttac atctgtcatg ctgttccttc
    21661 ctgctacagt ttattacgct tcgtcaccga accagcaaat attacgggca gccataatca
    21721 gtgctccggc tttttcgtcg gtcaaccctt cgatatcagc cagatcatca atgccctgtt
    21781 cggcgagatc ttccagcgta caaacgccac gggcggccag tttgaatgcc aaatcacgat
    21841 ctaccccttc aaggttcagc agatcgtcag ccggtttgtt atcaccgagg ctttcttcct
    21901 gggcctgtgc aatggtggcc agtgcatttt tagcacgctc gcgcagtgct tcaacggtcg
    21961 gctcatcaag gccttcgatt tccaacagct ctttcatcgg cacataggcc agttcttcca
    22021 gcgtcgagaa accttcttct accagaacag tcgcgaagtc ttcgtcgatg tcgagatatt
    22081 tggtgaaggt gtcgatcgct gcgtgcgctt ccgcctgatg cttagcctgc aggtcgtcaa
    22141 cggtcattac gttgagttcc caaccgctca gctgcgaagc cagacgcacg ttctgaccgt
    22201 tacggccaat cgcctgcgcc aggttaccag cttcaacggc gatatccatg gtgtgtttat
    22261 cttcatccac cacgatagaa gcaacgtctg ccggtgccat tgcgttaatc acaaactgcg
    22321 ccgggttatc atcccacagg acgatatcga tacgctcgcc gcccagttca gtagaaaccg
    22381 cctgaacacg cgcgccacgc atacctacgc aagcacctac cggatcgata cgtttatcgt
    22441 tggttttcac cgcgattttc gcacgagaac ccgggtcgcg agccgctgct ttaatttcaa
    22501 tcacttcttc gccgatttcc ggcacttcaa tacggaacag ttcgatcagc atttccggct
    22561 tggaacgagt gacgaacagt tgcgcgccac gcgcttccgg gcgaacggaa taaagcacgc
    22621 cacgaacgcg gtcgccaggg cggaagtttt cacgcggcag catatcttcg cgcaggatca
    22681 cggcttcagc gttgttgccc agatccagag agatgttgtc gcggtttact tttttcacca
    22741 cgccggtgat gatttcacct tcgtgttcac ggaactgatc aaccaccatc gcacgttcgg
    22801 cttcacgcac tttctgcacg ataacctgtt ttgccgtctg ggtggtgata cggtcaaagg
    22861 taacagactc aatctgatct tcaacgtaat cgcccaagtt caggctttca tcttcataac
    22921 gtgcggcttc aagggtgatt tccttggtcg gctgggtgac ttcatcaaca actaaccagc
    22981 gacggaaggt gtcaaaatca ccgcttttgc gatcgatctg tacacggacg tcgatctctt
    23041 gctcatattt tttctttgtt gctgtcgcca gcgcgctttc caatgcttcg aaaatcttct
    23101 cgcgaggtag cgccttttca ttggatacgg cttcaactac agccaaaatt tctttgttca
    23161 tcgcgggctt ttcacctcat ccagactatt aaaagtgggg aaccaggttc gccttctgga
    23221 tattactcag cgcgaacact tcatctttac cttcgacggt aactgtgatc atttcaccgt
    23281 ctaccgcttt gataacgccc tgccatttac gacggttttg taccgccata cggagaacca
    23341 gagtcacctc ttctccgaca aaacgggcgt agtgttcagc cgtgaacagt gggcgatcga
    23401 gacccggtga ggagacttcc aggttataag caacggtgat gggatcttca acatccagca
    23461 cagcacttac ctggtggctc acatcagcac aatcatcaac attgatgcca tcttcactat
    23521 caatatagat gcgcagtgtg gatgtgcgac cgcgaataaa ttcgatgcct accagttcaa
    23581 aacccagggc ctcaactggc gcagtaatca tctctgttaa tttttgctct aatgtggaca
    23641 agcccacccc caagacataa aaaaagggcc taaagcccag ttattctgta gtcagataac
    23701 aaaaaacccc gataaatcgg ggctttatat aactgaaccc tataaccgca actgcggtct
    23761 ggagcacttt ccagaaggat tttttcaaat cccactacga aggccgaagt cttcacagta
    23821 tatttgaaaa aggactctaa gggaaa
//
LOCUS       DADLTY010000051        22984 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.051, whole genome shotgun sequence.
ACCESSION   DADLTY010000051 DADLTY010000000
VERSION     DADLTY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22984
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.051"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..333)
                     /locus_tag="JN334_001705"
     CDS             complement(<1..333)
                     /locus_tag="JN334_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437873.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQK"
     gene            703..1653
                     /gene="corA"
                     /locus_tag="JN334_001706"
     CDS             703..1653
                     /gene="corA"
                     /locus_tag="JN334_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAY7437874.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1694..2074)
                     /locus_tag="JN334_001707"
     CDS             complement(1694..2074)
                     /locus_tag="JN334_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HAY7437875.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2088..2504)
                     /locus_tag="JN334_001708"
     CDS             complement(2088..2504)
                     /locus_tag="JN334_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437876.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2563..3453)
                     /gene="rarD"
                     /locus_tag="JN334_001709"
     CDS             complement(2563..3453)
                     /gene="rarD"
                     /locus_tag="JN334_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HAY7437877.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3505..3972)
                     /locus_tag="JN334_001710"
     CDS             complement(3505..3972)
                     /locus_tag="JN334_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HAY7437878.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4137..5006
                     /gene="pldA"
                     /locus_tag="JN334_001711"
     CDS             4137..5006
                     /gene="pldA"
                     /locus_tag="JN334_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HAY7437879.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5139..6968
                     /gene="recQ"
                     /locus_tag="JN334_001712"
     CDS             5139..6968
                     /gene="recQ"
                     /locus_tag="JN334_001712"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAY7437880.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7032..7652
                     /gene="rhtC"
                     /locus_tag="JN334_001713"
     CDS             7032..7652
                     /gene="rhtC"
                     /locus_tag="JN334_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HAY7437881.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7714..8334)
                     /gene="rhtB"
                     /locus_tag="JN334_001714"
     CDS             complement(7714..8334)
                     /gene="rhtB"
                     /locus_tag="JN334_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HAY7437882.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8445..9467
                     /gene="pldB"
                     /locus_tag="JN334_001715"
     CDS             8445..9467
                     /gene="pldB"
                     /locus_tag="JN334_001715"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HAY7437883.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9475..10275
                     /locus_tag="JN334_001716"
     CDS             9475..10275
                     /locus_tag="JN334_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAY7437884.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10351..11250
                     /gene="bioP"
                     /locus_tag="JN334_001717"
     CDS             10351..11250
                     /gene="bioP"
                     /locus_tag="JN334_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HAY7437885.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11138..12091)
                     /gene="metR"
                     /locus_tag="JN334_001718"
     CDS             complement(11138..12091)
                     /gene="metR"
                     /locus_tag="JN334_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HAY7437886.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12327..14588
                     /gene="metE"
                     /locus_tag="JN334_001719"
     CDS             12327..14588
                     /gene="metE"
                     /locus_tag="JN334_001719"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAY7437887.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14627..15442)
                     /locus_tag="JN334_001720"
     CDS             complement(14627..15442)
                     /locus_tag="JN334_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY7437888.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15704..16465
                     /gene="udp"
                     /locus_tag="JN334_001721"
     CDS             15704..16465
                     /gene="udp"
                     /locus_tag="JN334_001721"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HAY7437889.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16606..18033
                     /gene="rmuC"
                     /locus_tag="JN334_001722"
     CDS             16606..18033
                     /gene="rmuC"
                     /locus_tag="JN334_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAY7437890.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18128..18883
                     /gene="ubiE"
                     /locus_tag="JN334_001723"
     CDS             18128..18883
                     /gene="ubiE"
                     /locus_tag="JN334_001723"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAY7437891.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18897..19502
                     /gene="ubiJ"
                     /locus_tag="JN334_001724"
     CDS             18897..19502
                     /gene="ubiJ"
                     /locus_tag="JN334_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HAY7437892.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19499..21139
                     /gene="ubiB"
                     /locus_tag="JN334_001725"
     CDS             19499..21139
                     /gene="ubiB"
                     /locus_tag="JN334_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAY7437893.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21218..21487
                     /gene="tatA"
                     /locus_tag="JN334_001726"
     CDS             21218..21487
                     /gene="tatA"
                     /locus_tag="JN334_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HAY7437894.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21491..22006
                     /gene="tatB"
                     /locus_tag="JN334_001727"
     CDS             21491..22006
                     /gene="tatB"
                     /locus_tag="JN334_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HAY7437895.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22009..22785
                     /gene="tatC"
                     /locus_tag="JN334_001728"
     CDS             22009..22785
                     /gene="tatC"
                     /locus_tag="JN334_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HAY7437896.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22827..>22984
                     /locus_tag="JN334_001729"
     CDS             22827..>22984
                     /locus_tag="JN334_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="magnesium dependent; involved in quality control of
                     mutated Tat exported substrates; not involved in the
                     Sec-independent protein export system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase TatD"
                     /protein_id="HAY7437897.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYS"
BASE COUNT         5526 a         5706 c         6150 g         5602 t
ORIGIN      
        1 cttctgctga ccgttttcga tgtacaaacc gagcggcgca tagctttcat catagatgcc
       61 gccgttcatc gccatctgca cctgaccctg actattaata tccgccagca gagcatgtaa
      121 cgttccccac gcttcgccat tggctttttg ccagtacatt ttcacccgct ctgtttgagg
      181 attaacggta tacgcctgta cggtcagcgt cggatctgag agtgcgcaat catcagcggc
      241 aacagcaaac aagggaagta aggtgagggt gagaaaaatc cgtttgagat tcaaggtgat
      301 cattccttta ccaatgagta gccgatgcgc cattataggt cctagatgtg ggattttttt
      361 atcctgttag cgaccttgac gagtaccaaa aagcgcgaag ttcaactatt gttctgtggt
      421 gttctgttgc gtgttgacgg caaaattttg ctggcgtaac atgcgcgcac gatcactcta
      481 agaggacatt cgccttggac acacccagta gatactggct cactatcctg tcatccagga
      541 tcaactccta aggctatccc tttttgctga tagccttagc ggttgtcagc gacctcaatt
      601 tttcccgtcg cgctgagtca ggctgtttaa tggtctgaaa cccaatttgt ttctgtgtgc
      661 ccaccgaact gtccgatatt ttaagcattg ggagtcccgg tcatgctgag cgcatttcaa
      721 ctggaaaata accgactgac ccggctggaa gtcgaagagt cacaacccct tgtaaatgca
      781 gtatggattg atcttgtcga accggacgac gacgagcgac tgcgcgtaca atctgaactt
      841 ggccagagcc tggcaacccg cccggaactg gaagacatcg aagcatcggc acgtttcttt
      901 gaagacgacg acggcctgca tattcactcc ttcttcttct ttgaagatgc ggaagatcac
      961 gccggtaact ccactgtggc atttaccatc cgtgatggtc gtctgtttac tctgcgtgag
     1021 cgtgaactgc ccgcttttcg tctgtatcgt atgcgtgccc gtagccagtc gatggtagac
     1081 ggtaacgcct acgagttgct gctggatctg ttcgaaacca aaatcgaaca gttggcagat
     1141 gaaattgaaa atatctatag cgacctggag cagttgagcc gggtgattat ggaagggcat
     1201 cagggcgatg agtacgacga ggcgctctcc acgctggcgg aactggaaga tatcggctgg
     1261 aaagttcgcc tgtgtctgat ggatacccag cgcgctctca acttcctggt gcgtaaagcg
     1321 cgtttaccgg gtgggcaact ggagcaggcg cgtgaaatcc tgcgcgatat cgaatccctg
     1381 ctgccgcata acgaatccct gttccagaag gtgaacttcc tgatgcaggc ggcaatgggt
     1441 tttatcaaca tcgagcagaa ccgcatcatc aaaattttct cggtggtatc cgtcgtgttc
     1501 ctgccgccaa cgctggttgc ttccagctac gggatgaact ttgagtttat gccagaactg
     1561 aagtggagtt tcggctaccc tggcgcgatt atctttatga tcctcgcggg cctggcaccg
     1621 tatctgtact ttaagcggaa gaactggttg taaaaagaag gcggtggctt agcctggcta
     1681 agccacctgt tattcaaagg ctccaggtat ttaacccttt tacctctttc tcataaaacc
     1741 atttgttcgt gttaacagca acataggctg ctacggcaat tcccagaatg ttaacgccaa
     1801 ttaatgcacc aacaaataga gatagaatgc caagtaatag aactataatt gcttttttcc
     1861 ataagcccag aacaaataaa tagatccagg aacagaagaa agcaatgaaa ttcatttgaa
     1921 tagttaatcg ttgtcttact tttaatgctt taaatgctgc tttatattct ggtgttgctc
     1981 cccagaatcc aggaaagcca tgttgatcat aaaaattaaa tcggtatttc catttttcac
     2041 ttaatgaatc atcgttcata tattccttac tcataaatac tccataacta ttgttttgat
     2101 gaatcagtag gtgcaagcat tagcatactg aaagtggaaa aataacaaat caaaaaaatc
     2161 atcgaaccat tgcctgaaca ggcaaaatct tcggctatca ttgtgatgat agagatgata
     2221 tatactgcta atgtaccaaa aacataaatt tttatatata tgaaaccact atcacggagt
     2281 cgttggcaat tcatgttgat gacgagataa tggagtgcga tggtagagac tataacaaga
     2341 aaacctgctt ccccatcgtt aaaaaagata ataagaaagg caaaaatgaa atttattaaa
     2401 ataaataaaa atatatatcg gtgtctggaa atcttaccgt tagatgttgg gataaacata
     2461 cgtaacataa attttacatc cttgtgtgag tctcctgtca gcatggcaat atgcccactc
     2521 tcatacaaga gtcggcatat tttttcagaa tatatttatt ttttatttgg acgttctacg
     2581 ctgcgtataa atcgcgtcca tcacaaaaat tgccagcgcc acccaaataa aggtgaaagt
     2641 caccatctta tcggcacccg gtttttcacc ataaaacgtc acagccagca ggaacatcag
     2701 cgtcgggcca atgtactgaa aaaagcctaa cgttgagaga cgcaggcgcg tggcagcggc
     2761 agtaaaacac aacagcggta cggtagtgac aataccggcg gcgatcagca gtaaattcag
     2821 cgacatcggg ttttgcccca tatggctggt tgaactgtcg gcaatagcaa acaggtaaat
     2881 cgccgccacg ggcagcagcc acatggtttc aattaacatg ccggtttgtg cttctaccgc
     2941 aatcttcttg cgcaccaggc cgtagaaggc aaaactaaat gccagcccca gcgcgataat
     3001 aggtagcgaa ccaaaagtcc acagctggac caacacgcca catatcgcca gaatcaccgc
     3061 cagccattgc atccggcgga atcgctcgcc gaggaaaatc atccccagca caatgttcac
     3121 cagcgggtta ataaagtaac caaggctcgc ttccagcata tggtgattgt tcaccgccca
     3181 gataaacagt agccagttgc caccaatcag tacggcagag actgccagca taaaaatttt
     3241 ctgtggcgtc tgaatcagcg tttttaaata ggaccactgg cggcaaatgc tcatcagcac
     3301 caccataaag aaaaacgacc agatcacgcg atgcgtcagg atctcatcgg cgggcacgta
     3361 gtaaatcagc ttgaagtatg ctggcgcaat accccaaata aaataagcgg caagagcgag
     3421 taatacgccc tgccgcgttt gttttgcatc catcgggaat actcattttt aattggtaac
     3481 agcagtttac ctgcttttat gtcttcaacc taccatatag gtggcggtgg cactggcaat
     3541 ataaagctgt tcttcattgt gtaattcaac gcgggcgacg gcgactttat tgcctgcacg
     3601 caacaggcta ctggtagcag taaaacgctc gcccctgcct gggcgcagat aatcaacgcg
     3661 aagatcaatg gtccccatcc gcgatagccg ctggcgtagt tcatcttcac tgatggtttc
     3721 gtggcgggtt aaggtacttc ccacgcacac cagaccggcg gcgacatcca gcgccgacgc
     3781 aatgactccg ccgtgcaaaa tgctttgcgc ccagttaccc accatcatcg gctgattttt
     3841 aaaggccagc tgtgcgaact ctttttcgta acgctccagt tccatcccca atgcgcggtt
     3901 aaatggcatg tgataaacaa acatctcacc cactaatttc agggcttgtt cagcggtcag
     3961 tacggcagac atatcatcct tacacttcat tggttaatga aatgttgatt ttatgcttct
     4021 ttgttgttgg tttctacttt aggaagggat aactaacggc aatggagtta agtatgtaaa
     4081 atagcccgca gaaaaacatt caccttatca ataattcgtt acggagaaca cgaccgatgc
     4141 ggactctgca gggctggttg ttgccggtgt ttatgttgcc tatggcagta tatgcacaag
     4201 aggcaacggt gaaagaggtg catgacgcgc cagcggtgcg tggcagtatt atcgccaata
     4261 tgctgcagga gcatgacaat ccgttcacgc tctatcctta tgacaccaac tacctcatct
     4321 acacccaaac cagcgatctg aataaagaag cgattgccag ttacgactgg gcggaaaatg
     4381 cgcgtaagga tgaagtaaag tttcagttga gcctggcatt tccgctgtgg cgtgggattt
     4441 taggcccaaa ctcggtgttg ggtgcgtctt atacgcaaaa atcctggtgg caactgtcca
     4501 atagcgaaga gtcttcaccg tttcgtgaaa ccaactacga accgcaattg ttcctcggtt
     4561 ttgccaccga ttaccgtttt gcaggttgga cgctgcgcga tgtggagatg gggtataacc
     4621 acgactctaa cgggcgttca gacccgacct cccgcagctg gaaccgcctt tatactcgcc
     4681 tgatggcaga aaacggtaac tggctggtag aagtgaagcc gtggtatgtg gtgggtaata
     4741 ctgacgataa cccggatatc accaaatata tgggttacta ccagcttaaa atcggctatc
     4801 acctcggtga tgcggtgctc agtgcgaaag gacagtacaa ctggaacacc ggctacggcg
     4861 gcgcggagtt aggcttaagt tacccgatca ccaaacatgt gcgcctttat actcaggttt
     4921 acagcggcta tggcgaatcg ctcatcgact ataacttcaa ccagacccgt gtcggtgtag
     4981 gggttatgct aaacgacttg ttttgatgaa cggttgagtg gttggcaaat ctggaatcca
     5041 gcatccagga ttaccctctc agagactaaa agcattgcag tttctcgcgc aggcgctgaa
     5101 aatagcgcct gtttttattt caggcaatcg gggtgaatgt ggcgcaggcg gaagtgttga
     5161 atctggagtc cggagctaaa caggttttac aagaaacctt tggctaccaa cagtttcgcc
     5221 ccggccagga agaaattatc gacactgtgc tttccggtcg cgattgcctg gttgttatgc
     5281 ccactggtgg cggaaaatcc ctttgctatc aaattcctgc cttattgcta aacggcctta
     5341 ccgtggttgt ttcaccgctg atttcgttga tgaaagatca ggtggatcaa ctacaggcca
     5401 acggcgtggc ggcggcgtgc cttaactcga cgcaaacccg tgaacagcaa cttgaagtga
     5461 tgacaggctg ccgcaccggg caaattcgct tactgtatat cgccccggaa cgcctgatgc
     5521 tggataactt tcttgagcat ctggcgcact ggaatccggt gttattagcc gtcgatgaag
     5581 cgcactgtat ctcccaatgg ggtcacgatt tccgcccgga atatgccgcg ctcggtcagt
     5641 tgcgccagcg gttcccgacg ctgccgttta tggcgctgac cgccacagcc gacgacacca
     5701 cgcgccagga tatcgtgcgc ctgctggggc tgaacgatcc gctgattcaa atcagcagtt
     5761 ttgaccgtcc gaatattcgc tacatgctga tggagaaatt caaaccgctc gatcagttga
     5821 tgcgctacgt gcaggaacag cgcggtaagt ctggcattat ctactgcaac agccgggcga
     5881 aagtagaaga caccgctgcg cgcctgcaaa gcaggggtat tagcgcggcg gcctatcatg
     5941 ccgggctgga aaataatgtc cgcgccgacg tgcaggagaa attccagcgc gatgatctgc
     6001 aaattgtggt ggcgacggtg gcgttcggca tgggcatcaa taaaccaaac gttcgcttcg
     6061 tggtccactt tgatattccg cgcaatatcg aatcctatta tcaggaaacc ggtcgcgccg
     6121 ggcgtgatgg cctgcctgcg gaagcgatgc tgttttacga tccggctgat atggcgtggc
     6181 tgcgccgttg tctggaagag aagccgcagg ggcagttgca ggatatcgag cgccacaaac
     6241 tcaatgcgat gggcgcgttt gccgaagcgc aaacttgccg tcgtctggta ttgctgaact
     6301 attttggcga agggcgtcag gagccgtgcg ggaactgcga tatctgcctc gatccgccga
     6361 aacagtacga cggttcaacc gatgctcaga ttgccctttc caccattggt cgtgtgaatc
     6421 agcggtttgg gatgggttat gtggtggaag tgattcgtgg tgctaataac cagcgtatcc
     6481 gcgactatgg tcatgacaaa ctgaaagtct atggtatggg ccgtgataaa agccatgaac
     6541 attgggtgag cgtgatccgc cagctgattc atctcggcct ggtgacgcaa aatattgccc
     6601 agcattctgc cctacaactg acagaggccg cgcgcccggt gctgcgcggc gaatcctctt
     6661 tgcaacttgc cgtgccgcgt atcgtggcgc tcaaaccgaa agcgatgcag aaatcgttcg
     6721 gcggcaacta tgatcgcaaa ctgttcgcca aattacgcaa actgcgtaaa tcgattgccg
     6781 atgaaagtaa tgtcccgccg tacgtggtgt ttaacgacgc aaccttgatt gagatggctg
     6841 aacagatgcc gatcaccgcc agcgaaatgc tcagcgttaa cggcgttggg atgcgcaagc
     6901 tggaacgctt tggtaaaccg tttatggcgc tgattcgtgc gcatgtcgat ggcgacgacg
     6961 aagagtagtc agcagcataa aaaagtgcca gtatgaagac tccgtaaacg tttcccccgc
     7021 gagtcaaatg tatgttgatg ttatttctca ccgtcgccat ggtgcacatt gtggcgctta
     7081 tgagccccgg tcccgatttc ttttttgtct ctcagaccgc tgtcagtcgt tcccgtaaag
     7141 aagcgatgat gggcgtgctg ggcattacct gcggcgtaat ggtttgggct gggattgcgt
     7201 tgcttggcct gcatttgatt atcgaaaaaa tggcctggct gcatacgctg attatggtag
     7261 gcggtggcct gtatctctgc tggatgggtt accagatgct acgcggtgca ctgaaaaaag
     7321 aggctgtatc tgcgcctgca ccacaggttg agctggcgaa aagcgggcgc agtttcctga
     7381 aaggcttact gaccaatctc gccaatccga aagcgattat ctattttggt tcggtattct
     7441 cattgtttgt cggcgataac gttggcacta ccgagcgctg gggcattttc gcgttgatta
     7501 ttgtcgaaac actggcgtgg tttaccgtcg ttgccagtct gtttgccctg ccgcaaatgc
     7561 gccgtggtta tcaacgtctg gcgaagtgga ttgatggttt tgccggggcg ttatttgccg
     7621 gatttggcat tcatttgatt atttcgcggt gatgccagac gcgtcttcag agtaagtcgg
     7681 ataaggcgtt tacgccgcat ccgacattat ttttcacgca tgcctcgccg atgctaacag
     7741 cgctcccacc agcataaaca acgagccgaa aatcttattc agcgccttca tctgctttgg
     7801 tcctttaatc catagagcaa tcctttgagc aagggtggcg taaccgatca tcacaataat
     7861 atcgaccaca atagtggtga cgccgagcac gatatactgc atcagttgcg gctgttgcgg
     7921 catgatgaat tgcggaaata gcgccgccag aaacacaata cttttgggat tggtgagatt
     7981 cacaaaaact gcgcgctgga acaaatgtcg acgcgattga gtagaggcca gcgatttaag
     8041 atcaattgcg ccagcggcgc gccactgctg gattcccagc caaatcaagt aagccgcgcc
     8101 tgcccacttt aacacttcaa acgcaatcac tgagcgggaa aatagcgtcc ccaaccccac
     8161 gccaaccagc acaatatgaa tcgccagtcc ggtctgaagc ccagcaatag acgccaccgc
     8221 gccgcgataa ccgtggttga gcgaggtggt catagtgttg attgcaccag agcctggcga
     8281 caggcttaaa atgatcgatg tcagcaggta ggcaaaccac cattctaagg tcatgatgaa
     8341 ctcccggtgt gtctattttt gtgccacaat acgctactgt cgcagcgttg tgtcaggcac
     8401 gcttaaaaaa acgattttac gtggtttaag aggcagatta cccgatgttt cagcagcaaa
     8461 aagactggga aacaagagaa aacgcgtttg ctgcttttac catgggaccg ctgactgatt
     8521 tctggcgtca gcgtgatgaa gcagagttta ctggtgtgga tgacattccg gtgcgctttg
     8581 tccgttttcg cgcacagcac catgaccggg tggtagtcat ctgcccgggg cgtattgaga
     8641 gctacgtaaa atatgcggaa ctggcctacg acctgttcca tttggggttt gatgtcttaa
     8701 tcatcgacca tcgcgggcag ggacgttccg gtcgcctgtt agccgatccg catctcgggc
     8761 atgttaatcg ctttaatgat tatgttgatg atctggcggc attctggcag caggaggttc
     8821 agcccggtcc gtggcgtaaa cgctatatac tggcacattc gatgggcggt gcgatctcca
     8881 cattatttct gcaacgccat ccaggtgtat gtgacgccat tgcgctaact gcgccaatgt
     8941 ttgggatcgt gattcgtatg ccgtcattta tggcacggca gatcctcaac tgggccgaag
     9001 cgcatccacg tttccgtgat ggctatgcaa taggcaccgg gcgctggcgc gcgttgccgt
     9061 ttgctatcaa cgtactgacc cacagcagac agcgatatcg acgtaactta cgcttctatg
     9121 ctgatgaccc aacgattcgc gtcggtgggc cgacctacca ttgggtacgc gaaagtattc
     9181 tggctggcga acaggtgtta gccggtgcgg gtgatgacgc cacgcaaacg cttctcttac
     9241 aagctgaaga ggaacgcgtg gtggataacc gcatgcacga ccgtttttgt gaactccgca
     9301 ccgccgcggg ccatcctgtc gaaggaggac ggccgttggt aataaaaggt gcttaccatg
     9361 agatcctttt tgaaaaggac gcaatgcgct cagtcgcgct ccacgccatc gttgattttt
     9421 tcaacaggca taactcaccc agcggaaacc gctctacaga ggtttaaatt tcttatgtac
     9481 caggttgttg cgtctgattt agatggcacg ttactttctc ccgaccatac gttatcccct
     9541 tacgccaaag aaactctgaa gctgctcacc gcgcgcggca tcaactttgt gtttgcgacc
     9601 ggtcgtcacc acgttgatgt ggggcaaatt cgcgataatc tggagattaa gtcttacatg
     9661 attacctcca atggtgcgcg cgttcacgat ctggatggca atctgatttt tgctcataac
     9721 ctggatcgcg acattgccag cgatctgttt ggcgtagtca acgacaatcc ggacatcatt
     9781 actaacgttt atcgcgacga cgaatggttt atgaatcgcc atcgcccgga agagatgcgc
     9841 ttttttaaag aagcggtgtt caaatatgcg ctgtatgagc cgggattact ggagccggaa
     9901 ggcatcagca aagtgttttt cacctgcgat tcccatgaac aactgctgcc gctggagcag
     9961 gcgattaacg ctcgctgggg cgatcgcgtc aacgtcagtt tctctacctt aacctgtctg
    10021 gaagtgatgg cgggcggcgt gtcgaaaggc catgcgctgg aagcagtggc gaagaaactg
    10081 ggctacagcc tgaaggattg tattgcgttt ggtgacggga tgaacgacgc cgaaatgctg
    10141 tcgatggcgg ggaaaggctg cattatgggc agtgcgcacc agcgtctgaa agatctgcat
    10201 ccggagctgg aagttattgg tactaatgcc gacgacgcgg tgccgcatta tctgcgtaaa
    10261 ctctatttat cgtaatcgtt ctttatttgg tcagttgtca acctgatact tcgctacaat
    10321 ggatactcgt taatcaaaga gttttccatt gtggcgttac ttatcatcac cacgattctg
    10381 tgggccttct cctttagctt ttatggcgag taccttgcgg ggcacgtcga tagctatttt
    10441 gcggtgctgg tgcgcgttgg cttggcggca ctcgtttttc tgccgtttct gcgtacccgt
    10501 agcaatagcc tgaaaacggt cggcctgtat atgctggtgg gcgcgatgca gctcggcgtg
    10561 atgtatatgc tgagtttccg cgcttatctc tacctgacgg tctccgaatt gttgctgttc
    10621 accgtactga cgccgctcta catcacgctg atttatgaca tcatgagtaa gcgccgtctg
    10681 cgctggggct atgcttttag cgccttgctg gcggtgattg gtgccgggat tattcgctat
    10741 gatcaggtca ccgaccattt ctggactggc ttgttgctgg tgcaactctc caatatcact
    10801 tttgccatcg gcatggtggg ttacaaacgc ctgatggaaa ctcgcccgat gccgcagtat
    10861 aacgcctttg cgtggttcta tcttggcgcg ttcctggtgg cggtaattgc atggttcttg
    10921 ctgggaaatg cgcagaaaat gccgcaaacc acgttgcaat ggggcattct ggtgtttctt
    10981 ggcgtggtgg cttccgggat tggctacttt atgtggaact acggcgcgac gcaggtggac
    11041 gccggaacgc tgggcattat gaataatatg cacgttccgg cagggctgct ggtaaacctg
    11101 gctatctggc accaacagcc gcactggcca acgtttatta caggcgcgct ggtgatcctg
    11161 gcctcactgt gggtgcatcg taagtgggtc gctccgcgct cttcacaaac ggcagatgat
    11221 cgcaggcgtg attgcgcgct gagcgaataa acgcttccgt aactggctga cgctgctcgc
    11281 catcgcgcac ggcggcgtac agtcggctcc acaagccttc gcccagggtt ttggtcacca
    11341 ccagaccctg gcgctcaaaa ctctctacta cccaatgcgg tagcgcggca atacccatcc
    11401 gcgcggcaac catttgaatc aacaataagg tgttatcgac gcttttcagt gacgggctga
    11461 cgcctgccgg ctgaagaaaa tgccgccaga catccagtcg actacgctgc accggataaa
    11521 ttaatagcgt ctcgctggcg agatcttccg gtgtaatacg cgtttttgcc gccagtggat
    11581 ggtcaggtgc taacaccagg cgtacttcat aatcgaacat cggcgaataa tgcaggccac
    11641 tgcgcggcag gatatcggag gtcatcacca gatccagctc gccctgttgc aacgcgggct
    11701 gcgggtcaaa cgtcacgccc gatttaaaat ccatctctac ctgcggccag ttcttatgga
    11761 aatttcctaa agcgggtgtc agccactgaa tacagctatg gcactcaatg gcaatgcgca
    11821 gacgcgtctg ctgcggttca ttgcaggctt gcagtgcctg gctgatttgc ggcagcacct
    11881 ggtttgccag ttgcaacagg atttctccct gcggtgtaaa gcgtagcggc tggctcttac
    11941 gcacaaatag ccggaagcca aggcgttgtt ccagatcgct aaactggtga gacagggcgg
    12001 attgcgtctg atgcaacgtt gccgcagcgg ctgcgagcga gccgcagttc cgcaacgctt
    12061 gtagcgtttt caggtgtttt acttcgatca tgaaagtcct tcacttcggc atgaataatt
    12121 tgcgcttgag gaatatacag taaccgccaa ttatggatgt gtaaacatct ggacggctaa
    12181 aatcattcgt cttttaaatt tatggtgcgt tggctgcgtt tctccacccc ggtcacttac
    12241 ttcagtaagc tcccggcgat gaataaactt gccgccttcc ctaaattcaa aatccatagg
    12301 atttacatat aattagagga agaaaaatga caatattgaa tcacaccctc ggtttccctc
    12361 gcgttggcct gcgtcgcgag ctgaaaaaag cgcaagaaag ttattgggcg gggaactcca
    12421 cgcgtgaaga actgctgaca gtagggcgtg aactgcgtgc tcgtcactgg gatcaacaaa
    12481 agcaagcggg tatcgacctg ctgccggtgg gcgattttgc ctggtacgat catgtactga
    12541 ccaccagttt gctgctgggt aatgttccgc cacgtcatca gaacaaagac ggttcggtag
    12601 atatcgacac cctgttccgt attggtcgtg gacgtgcgcc gactggcgaa cctgcggcgg
    12661 cagcggaaat gaccaaatgg tttaacacca actatcacta catggtgccg gagttcgtta
    12721 aaggccaaca gttcaaactg acttggacgc agctgctgga ggaagtggac gaggcgctgg
    12781 cgctgagcca caacgttaag cctgtgctgc tggggccggt tacttacctg tggctgggga
    12841 aagtgaaagg tgaacagttt gaccgcctta gcctgctgaa cgacattctg ccggtttatc
    12901 agcaagtgct ggcagaactg gcgaaacgcg gcatcgagtg ggtacagatt gatgaacccg
    12961 cgctggtact ggaactacca caggcgtggc tggacgcata caaacccgct tacgacgcgc
    13021 tccagggaca ggtgaaactg ctgctgacca cctattttga aggcgtaacg ccaaatctcg
    13081 acacgattac tgcgctgcct gttcaggggc tgcatgtgga ccttgtacat ggtaaagata
    13141 acgttgttga actgcacaag cgcctgcctt ctgactggct gctgtctgcg ggtctgatca
    13201 atggtcgtaa cgtctggcgc gccgatctta ctgagaaata tgcgcaaatt aaggacattg
    13261 tcggcaaacg tgatttgtgg gtggcatctt cctgctcgtt gctgcacagc cccatcgacc
    13321 tgagcgtgga aacgcgtctt gatgcagaag tgaaaagctg gtttgccttc gccctacaaa
    13381 aatgccatga actggcactg ctgcgcgatg cgctgaacag tggtgacacg gcagctctgg
    13441 cagagtggag cgccccaatt caggcgcgtc gtcactctac tcgtgtacat aatccggcgg
    13501 tagaaaagcg tctggcggcg atcaccgccc aggacagcca gcgtgcgaat gtctatgaag
    13561 tgcgtgctga agcccagcgt gcgcgtttta aactgccagc gtggccgacc accacgattg
    13621 gttccttccc gcaaaccacg gaaattcgta ccctgcgtct ggatttcaaa aagggcaatc
    13681 tcgacgccaa caactaccgc acgggcattg cggaacatat caagcaggcc attgttgagc
    13741 aggaacgttt gggactggat gtgctggtac atggcgaggc cgagcgtaat gacatggtgg
    13801 aatactttgg cgagcacctc gacggatttg tctttacgca aaacggttgg gtacagagct
    13861 acggttcccg ctgcgtgaag ccaccgattg tcattggtga cattagccgc ccggcaccga
    13921 ttaccgtgga gtgggcgaag tatgcgcaat cgctgaccga caaaccggtg aaagggatgc
    13981 tgacggggcc ggtgaccata ctctgctggt cgttcccgcg tgaagatgtc agccgtgaaa
    14041 ccatcgccaa acagattgcg ctggcgctgc gtgatgaagt ggccgatctg gaagccgctg
    14101 gaattggcat catccagatt gacgaaccgg cgctgcgcga aggtttaccg ctgcgtcgta
    14161 gcgactggga tgcgtatctc cagtggggcg tagaggcctt ccgtatcaac gccgccgtgg
    14221 cgaaagatga cacacaaatc cacactcaca tgtgttattg cgagttcaac gacatcatgg
    14281 attcgattgc ggcgctggac gcagacgtca tcaccatcga aacctcgcgt tccgacatgg
    14341 agttgctgga gtcgtttgaa gagtttgatt atccaaatga aatcggtcct ggcgtctatg
    14401 acattcactc gccaaacgta ccgagcgtgg aatggattga agccttgctg aagaaagcgg
    14461 caaaacgcat tccggcagag cgcctgtggg tcaacccgga ctgtggcctg aaaacgcgcg
    14521 gctggccaga aacccgcgcg gcactggcga acatggtgca ggcggcgcag aatttgcgtc
    14581 gggggtaaat ccaaaccggg tggtaatacc acccggtctt ttctcattac agcgacttct
    14641 tcccaccata ctgcttaaac cattccagca tacgctgcca gccatctttc gcagattcgg
    14701 catgatagct cgggcgataa tcagcgttga atgcatgccc ggcatccggg tacacgataa
    14761 tctctgcttt cgcattagca gcccgcagcg cctggcgcat cgtttcaacg ctctcctgcg
    14821 gaatgctgtt atcctgacca ccatataagc cgagaaccgg cgcgttaaga tcggttgcga
    14881 tatcaacagg ttgtttcggt gaattcagcg acttgtcgcc cgtcagtttg ccgtaccacg
    14941 ccactgcggc ttttagctgt ggattatgcg cggcatacag ccaggtgata cgtccacccc
    15001 agcagaatcc ggtgatcatt aaacgatgaa catcgccgcc gttgcgggaa gcccaactgg
    15061 cgacatgatc gagatcggcc agcacctgcg agtcaggcac ttttgctacc agaccgctaa
    15121 gcaacgtggg gatatcggca aaatcattcg gatcgccttc gcggaagtaa agttcaggtg
    15181 cgatagccag atacccctcc agcgccagac ggcgacaaat gtcgcggata tgttcatgca
    15241 cgccaaaaat ttcctgcact acaatgacca ctggcagtgg gccatcgctt tgctttggtc
    15301 tggcatggta agcaggcatg ttatcccctt gtgaagggat agaggtgaag cccgccacaa
    15361 tcgcgtcgtc cggggtctga acgatggtcg aagcgagagg cgatgcagca ggtgcaaatc
    15421 cagattgttg tgttgttgcc atggtattct ccgtaccctt ataaaaatgt tgcgcaatgt
    15481 taactatagt cagcatgcaa caaatcacat tgcctgaatc ggctcatctt ttatgcagtc
    15541 ctgcagaatg aagagtgatt tatgtgatat gaatcacttt tggtgggtaa atttatgcaa
    15601 cgcatttgcg tcatggtgat gagtatcacg aaaaaatgtt aaacccttcg gtaaagtgtc
    15661 tttttgcttc ttctgactaa accgattcac agaggagttg tatatgtcca agtctgatgt
    15721 ttttcatctc ggcctcacta aaaacgattt acaaggggct acgcttgcca tcgtccctgg
    15781 cgacccggat cgtgtggaaa agatcgccgc gctgatggat aagccggtta agctggcatc
    15841 tcaccgcgaa ttcactacct ggcgtgcaga gctggatggt aaacctgtta tcgtctgctc
    15901 taccggtatc ggcggcccgt ctacctctat tgctgttgaa gagctggcac agctgggcat
    15961 tcgcaccttc ctgcgtatcg gtacaacggg cgctattcag ccgcatatta atgtgggtga
    16021 tgtcctggtt accacggcgt ctgtccgtct ggatggcgcg agcctgcact tcgcaccgct
    16081 ggaattcccg gctgtcgctg atttcgaatg tacgactgcg ctggttgaag ctgcgaaatc
    16141 tattggcgcg acaactcacg ttggcgtgac agtttcttct gataccttct acccaggtca
    16201 ggaacgttac gatacttact ctggtcgcgt agttcgtcac tttaaaggtt ctatggaaga
    16261 gtggcaggcg atgggcgtaa tgaactatga aatggaatct gcaactctgc tgaccatgtg
    16321 tgcaagtcag ggcctgcgtg ccggtatggt agcgggtgtt atcgttaacc gcacccagca
    16381 agagatcccg aatgctgaga cgatgaaaca aaccgaaagc catgcggtga aaatcgtggt
    16441 ggaagcggcg cgtcgtctgc tgtaattctc ttctcctgtc tgaaggccga cgcgttcggc
    16501 cttttgtatt tttgcgtagc gcctcgcagg aaatgccttt ccaactggac gtttgtacag
    16561 cacaattcta ttttgtgcgg gtaagttgtt gcgtcaggag gcgttgtgga tttctcaatc
    16621 atggtttacg cagttattgc gttggtgggt gtggcaattg gctggctgtt tgccagttat
    16681 caacatgcgc agcaaaaagc cgagcaatta gctgaacgtg aagagatggt cgcggagtta
    16741 agcgcggcaa aacaacaaat tacccaaagc gagcactggc gtgcagagtg tgaattgctc
    16801 aataacgaag tgcgcagcct gcaaagtatt aacacctctc tggaggccga tctgcgtgaa
    16861 gtaaccacgc ggatggaagc cgcacagcaa catgctgacg ataaaattcg ccagatgatt
    16921 aacagcgagc agcgcctcag tgagcagttt gaaaacctcg ccaaccgtat ttttgagcac
    16981 agcaatcgcc gggttgatga gcaaaaccgt cagagtctga acagcctgtt gtcgccgcta
    17041 cgtgaacaac tggacggttt ccgccgtcag gttcaggaca gcttcggtaa agaagcacaa
    17101 gaacgccata ccctgaccca cgaaattcgc aatctccagc aactcaacgc gcaaatggcc
    17161 caggaagcga tcaacctgac gcgcgcgctg aaaggcgaca ataaaaccca gggcaactgg
    17221 ggcgaggtag tattgacgcg ggtgctggag gcttccggtc tgcgtgaagg gtatgaatat
    17281 gaaacccagg tcagcatcga aaatgacgcc cgctcgcgga tgcagccgga tgtcatcgtg
    17341 cggctgccgc agggaaaaga tgtggtgatc gacgccaaaa tgacgctggt cgcctatgaa
    17401 cgctatttta acgccgaaga cgactacacc cgcgaaagcg cgctacagga acatatcgcg
    17461 tcggtgcgta accatatccg tttgctggga cgcaaagatt atcaacagct gccggggctg
    17521 cgaactctgg attacgtgct gatgtttatt cccgttgaac ccgctttttt actggcgctt
    17581 gaccgccagc cggagctgat caccgaagcg ttgaaaaaca atatcatgct ggttagcccg
    17641 actacgctgc tggtggcgtt gcgtactatt gccaacctgt ggcgttatga gcatcaaagc
    17701 cgtaacgccc agcaaatcgc cgatcgtgcc agcaagctgt acgacaagat gcgtttgttc
    17761 atcgatgaca tgtccgcgat tggtcaaagt ctcgacaaag cgcaggataa ctaccgccag
    17821 gcaatgaaaa aactctcttc aggtcgcgga aatgtgctgg cgcaggcaga agcgtttcgc
    17881 ggtttaggag tagaaattaa acgcgagatt aatccggatt tggctgaaca ggcggtgagc
    17941 caggatgaag agtatcgact tcggtcggtt ccggagcagc cgaatgatga agcttatcaa
    18001 cgcgatgatg aatataatca gcagtcgcgc tagcccatta ggggtagtta agccgggtag
    18061 aaatctaggg catcgacgcc caatctgtta cacttctgga acaatttttt gatgagcagg
    18121 cattgagatg gtggataagt cacaagaaac gacgcacttt ggttttcaga ccgtcgcgaa
    18181 ggaacaaaaa gcggatatgg tcgcccacgt tttccattcc gtggcatcaa aatacgatgt
    18241 catgaatgat ttgatgtcat ttggtattca tcgtttgtgg aagcgattca cgattgattg
    18301 cagcggcgta cgccgtgggc agaccgtgct ggatctggct ggtggcaccg gcgacctgac
    18361 agcgaaattc tcccgcctgg tcggagaaac tggcaaagtg gtccttgctg atatcaatga
    18421 atccatgctc aaaatgggac gcgagaagct gcgtaatatc ggtgtgattg gcaacgttga
    18481 gtatgttcag gcgaacgctg aggcgctgcc gttcccggat aacacctttg attgcatcac
    18541 catttcgttt ggtctgcgta acgtcaccga caaagataaa gcactgcgtt caatgtatcg
    18601 cgtgctgaaa cccggcggcc gcctgctggt gcttgagttc tcgaagccaa ttatcgagcc
    18661 gctgagcaaa gcctatgatg catactcctt ccatgtgctg ccgcgtattg gctcactggt
    18721 cgcgaacgac gccgacagct accgttatct ggcagaatcc atccgtatgc atcccgatca
    18781 ggataccctg aaagccatga tgcaggatgc cggattcgaa agtgtcgact actacaatct
    18841 gacggcaggg gttgtggcgc tgcatcgtgg ttataagttc tgacaggaga ccggaaatgc
    18901 cttttaaacc tttagtgacg gcaggaattg aaagtctgct caacaccttc ctgtatcgct
    18961 cacccgcgct gaaaacagcc cgctcgcgtc tgctgggtaa agtattgcgc gtggaggtaa
    19021 aaggcttttc gacgtcattg attctggtgt tcagcgaacg ccaggttgat gtactgggcg
    19081 aatgggcagg cgatgctgac tgcaccgtta tcgcctacgc cagtgtgttg ccgaaacttc
    19141 gcgatcgcca gcagcttacc gcactgattc gcagtggtga gctggaagtg cagggcgata
    19201 ttcaggtggt gcaaaacttc gttgcgctgg cagatctggc agagttcgac cctgcggaac
    19261 tgctggcccc ttataccggt gatatcgccg ctgaaggaat cagcaaagcc atgcgcggag
    19321 gcgcaaagtt cctgcatcac ggcattaagc gccagcaacg ttatgtggcg gaagccatta
    19381 ctgaagagtg gcgtatggca cccggtccgc ttgaagtggc ctggtttgcg gaagagacgg
    19441 ctgccgtcga gcgtgctgtt gatgccctga ccaaacggct ggaaaaactg gaggctaaat
    19501 gacgccaggt gaagtacggc gcctatattt catcattcgc acttttttaa gctacggact
    19561 tgatgaactg atccccaaaa tgcgtatcac cctgccgcta cggctatggc gatactcatt
    19621 attctggatg ccaaatcggc ataaagacaa acttttaggt gagcgactac gactggccct
    19681 gcaagaactg gggccggttt ggatcaagtt cgggcaaatg ttatcaaccc gccgcgatct
    19741 ttttccaccg catattgccg atcagctggc gttattgcag gacaaagttg ctccgtttga
    19801 tggcaagctg gcgaagcagc agattgaagc tgcaatgggc ggcttgccgg tagaagcgtg
    19861 gtttgacgat tttgaaatca agccgctggc ttctgcttct atcgcccagg ttcataccgc
    19921 gcgattgaaa tcgaatggta aagaggtggt gattaaagtc atccgcccgg atattttgcc
    19981 ggtcattaaa gcggatctga aacttatcta ccgtctggct cgctgggtgc cgcgtttgct
    20041 gccggatggt cgccgtctgc gcccaaccga agtggtgcgc gagtacgaaa agaccttgat
    20101 tgatgaactg aatttgctgc gggaatctgc caacgccatt cagcttcggc gcaattttga
    20161 agacagcccg atgctctaca tcccggaagt ttaccctgac tattgtagtg aagggatgat
    20221 ggtgatggag cgcatttacg gcattccggt gtctgatgtt gcggcgctgg agaaaaacgg
    20281 cactaacatg aaattgctgg cggaacgcgg cgtgcaggtg ttcttcactc aggtctttcg
    20341 cgacagcttt ttccatgccg atatgcaccc tggcaacatc ttcgtaagct atgaacaccc
    20401 ggaaaacccg aaatatatcg gcattgattg cgggattgtt ggctcgctaa acaaagaaga
    20461 taaacgctat ctggcagaaa actttatcgc cttctttaat cgcgactatc gcaaagtggc
    20521 agagctacac gtcgattctg gctgggtgcc accagatacc aacgttgaag agttcgaatt
    20581 tgccattcgt acggtctgtg aacctatctt tgagaaaccg ctggccgaaa tttcgtttgg
    20641 acatgtactg ttaaatctgt ttaatacggc gcgtcgcttc aatatggaag tgcagccgca
    20701 actggtgtta ctccagaaaa ccctgctcta tgtcgaaggg gtaggacgcc agctttatcc
    20761 gcagctcgat ttatggaaaa cagcgaagcc tttcctggag tcgtggatta aagatcaggt
    20821 cggtattcct gccctggtga gagcatttaa agaaaaagcg ccgttctggg tcgaaaaaat
    20881 gccagaactg cctgaactgg tttacgacag tttgcgccag ggcaagtatt tacagcacag
    20941 tgttgataag attgcccgcg agcttcagtc aaatcatgta cgtcagggac aatcgcgtta
    21001 ttttctcgga attggcgcta cgttagtatt aagtggcaca ttcttgttgg tcagccgacc
    21061 tgagtggggg ctgatgcccg gctggttaat ggcaggtggt ctgatcgcct ggtttgtcgg
    21121 ttggcgcaaa acacgctgat tttttcatcg ctcaaggcgg gccgtgtaac gtataatgcg
    21181 gctttgttta atcatcatct accacagagg aacatgtatg ggtggtatca gtatttggca
    21241 gttattgatt attgccgtca tcgttgtact gctttttggc accaaaaagc tcggctccat
    21301 cggttccgat cttggtgcgt cgatcaaagg ctttaaaaaa gcaatgagcg atgatgaacc
    21361 aaagcaggat aaaaccagtc aggatgctga ttttactgcg aaaactatcg ccgataagca
    21421 ggcggatacg aatcaggaac aggctaaaac agaagacgcg aagcgccacg ataaagagca
    21481 ggtgtaatcc gtgtttgata tcggttttag cgaactgcta ttggtgttca tcatcggcct
    21541 cgtcgttctg gggccgcaac gactgcctgt ggcggtaaaa acggtagcgg gctggattcg
    21601 cgcgttgcgt tcactggcga caacggtgca gaacgaactg acccaggagt taaaactcca
    21661 ggagtttcag gacagtctga aaaaggttga aaaggcgagc ctcactaacc tgacgcccga
    21721 actgaaagcg tcgatggatg aactacgcca ggccgcggag tcgatgaagc gttcctacgt
    21781 tgcaaacgat cctgaaaagg cgagcgatga agcgcacacc atccataacc cggtggtgaa
    21841 agataatgaa gctgcgcatg agggcgtaac gcctgccgct gcacaaacgc aggccagttc
    21901 gccggaacag aagccagaaa ccacgccaga gccggtggta aaacctgctg cggacgctga
    21961 accgaaaacc gctgcacctt ccccttcgtc gagtgataaa ccgtaaacat gtctgtagaa
    22021 gatactcaac cgcttatcac gcatctgatt gagctgcgta agcgtctgct gaactgcatt
    22081 atcgcggtga tcgtgatatt cctgtgtctg gtctatttcg ccaatgacat ctatcacttg
    22141 gtatccgcgc cattgatcaa gcagttgccg caaggttcaa cgatgatcgc caccgacgtg
    22201 gcctcgccgt tctttacgcc gatcaagctg acctttatgg tgtcgctgat tctgtcagcg
    22261 ccggtgattc tctatcaggt gtgggcattt atcgccccag cgctgtataa gcatgaacgt
    22321 cgcctggtgg tgccgctgct ggtttccagc tctctgctgt tttatatcgg catggcattc
    22381 gcctactttg tggtctttcc gctggcattt ggcttccttg ccaataccgc gccggaaggg
    22441 gtgcaggtat ccaccgacat cgccagctat ttaagcttcg ttatggcgct gtttatggcg
    22501 tttggtgtct cctttgaagt gccggtggca attgtgctgc tgtgctggat ggggattacc
    22561 tcgtcagaag acttacgcaa aaaacgcccg tatgtgctgg ttggtgcatt cgttgtcggg
    22621 atgttgctga cgccgccgga tgtcttctcg caaacgctat tggcgatccc gatgtactgt
    22681 ctgtttgaaa tcggtgtctt cttctcacgc ttttacgttg gtaaagggcg aaatcgggaa
    22741 gaggaaaacg acgctgaagc agaaagcgaa aaaactgaag aataaattca accgcccgtc
    22801 agggcggttg tcatatggag tacaggatgt ttgatatcgg cgttaatttg accagttcgc
    22861 aatttgcgaa agaccgtgat gatgttgtag cgcgcgcttt tgacgcggga gttaatgggc
    22921 tactcatcac cggcactaac ctgcgtgaaa gccagcaggc gcaaaagctg gcgcgtcagt
    22981 attc
//
LOCUS       DADLTY010000052        22936 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.052, whole genome shotgun sequence.
ACCESSION   DADLTY010000052 DADLTY010000000
VERSION     DADLTY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.052"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JN334_001730"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JN334_001730"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAY7437898.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JN334_001731"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JN334_001731"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAY7437899.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JN334_001732"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JN334_001732"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAY7437900.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JN334_001733"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JN334_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HAY7437901.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JN334_001734"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JN334_001734"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HAY7437902.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JN334_001735"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JN334_001735"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY7437903.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="JN334_001736"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="JN334_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HAY7437904.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JN334_001737"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JN334_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY7437905.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JN334_001738"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JN334_001738"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAY7437906.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JN334_001739"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JN334_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HAY7437907.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /locus_tag="JN334_001740"
     CDS             complement(14847..15542)
                     /locus_tag="JN334_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY7437908.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="JN334_001741"
     CDS             complement(15665..16702)
                     /locus_tag="JN334_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7437909.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17024..17512
                     /gene="yhhY"
                     /locus_tag="JN334_001742"
     CDS             17024..17512
                     /gene="yhhY"
                     /locus_tag="JN334_001742"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7437910.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17571..19313)
                     /gene="ggt"
                     /locus_tag="JN334_001743"
     CDS             complement(17571..19313)
                     /gene="ggt"
                     /locus_tag="JN334_001743"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAY7437911.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19433..19873
                     /locus_tag="JN334_001744"
     CDS             19433..19873
                     /locus_tag="JN334_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HAY7437912.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19860..20603)
                     /gene="ugpQ"
                     /locus_tag="JN334_001745"
     CDS             complement(19860..20603)
                     /gene="ugpQ"
                     /locus_tag="JN334_001745"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY7437913.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20625..21512)
                     /gene="ugpC"
                     /locus_tag="JN334_001746"
     CDS             complement(20625..21512)
                     /gene="ugpC"
                     /locus_tag="JN334_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAY7437914.1"
                     /translation="MADGEFIVMVGPSGCGKSTLLRMVAGLERVTEGDIWINDQRVTE
                     MEPKDRGIAMVFQNYALYPHMSVEENMAWGLKIRGMGKQQIAERVKEAARILELDGLL
                     KRRPRELSGGQRQRVAMGRAIVRDPAVFLFDEPLSNLDAKLRVQMRLELQQLHRRLKT
                     TSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGTPVEVYEKPASLFVASFIGSPAMNLLT
                     GRMNNEGTHFELDGGIVLPLNGGYRQYAGRKMTLGIRPEHIALSSQAEAGGATGASGA
                     PDGRQHAVAASGGKSAASF"
     gene            complement(21592..22437)
                     /gene="ugpE"
                     /locus_tag="JN334_001747"
     CDS             complement(21592..22437)
                     /gene="ugpE"
                     /locus_tag="JN334_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAY7437915.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22434..>22936)
                     /gene="ugpA"
                     /locus_tag="JN334_001748"
     CDS             complement(22434..>22936)
                     /gene="ugpA"
                     /locus_tag="JN334_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HAY7437916.1"
                     /translation="FNPGRGLITHFLAEFGYDWNHAQNSGQAMFLVVFASVWKQISYN
                     FLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFLLVVNLVYAFFDTF
                     PVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFLVIVLTVVQFRYVE
                     SKVRYQ"
BASE COUNT         5555 a         6368 c         5764 g         5249 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcaca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctctaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagctgcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctacttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgaaagt
    16981 caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg agatagtaat
    17041 acgccacgca gaaacacggg attacgaggc catcaggcag attcacgccc agccggaggt
    17101 gtattgcaac acactacagg tgcctcatcc ttccgatcat atgtggcagg agcgactcgc
    17161 cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg tgggccatct
    17221 caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg gtatctgtgt
    17281 cgactctcgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga tgattgaaat
    17341 gtgcgacaac tggttacggg tagatcgcat tgaactaacc gtgtttgtcg ataacgcgcc
    17401 agcaattaag gtctataaaa aattcggctt tgagattgaa gggaccggta agaagtacgc
    17461 attgcgtaat ggtgaatatg tcgatgcgta ttatatggcg cgggtgaaat agagaatttg
    17521 caatctaagt ttgtcattta agccctcacc taccgggagg gcttaagata ttaatacccc
    17581 gccgttaaat catccaccga gcgcgggtcg gatgcgccgt acaactcacc gtccggccca
    17641 accataatgc tttgtgtgct gcccatcgcc tctttcagcg ccactttctg accttttgct
    17701 tccagcagct tgagcgtatc cgggctaaac cctttttcga cacgcagctc gtccggcaac
    17761 cactgatggt ggaaacgcgg cgcattggtc gcttcggcga cgttcatgcc ataatcgatg
    17821 ctattcacca ccatttgcag caccgtggtg ataatccggc taccgcctgg gctaccggta
    17881 accagccagg ttttaccgtc tttcaccaca atggtcggcg acatcgacga cagcgggcgt
    17941 ttgttcggcc cgacggcgtt ggcatcaccg cccaccagcc cgtaaacgtt cggtacgccc
    18001 ggtttggcgg agaaatcatc catctggtta ttaagcagaa taccgctctc acccgcgaca
    18061 atacccgtac cgaaggtggt gttcagcgta taggtcaccg ccaccgcgtt accgtcttta
    18121 tccactacag agtaatgggt agtttgatta ctctcataag gcgcaagctt gccagggcga
    18181 atctcactgg acggcttcgc ttttttgata tcaatttgat cggcaatgga tttggcatag
    18241 gctttattgg tcagtgcctg ccacgggact ttgacaaaat ccgggtcgcc aagatattcc
    18301 gagcggtcgg cgtaggcgta tttctccgct tctgccatga tttgcatcgc atcggcgctg
    18361 ccaaagccgt atttcttcat atcgaagttt tccagaatat tgaggatttg tacgatatgg
    18421 atcccgccgg aggatggcgg tggcatggag taaacctgat acccgcgata atcgccgctt
    18481 atcggagtgc gttcgaccgc tttataggcc gctaaatctt ctttagtgat caagccaccg
    18541 tttttctgca tctcctgggc gatctgttcc gcaatcgtgc ctttatagaa ttcgtccggg
    18601 ccgttttcag caatcatctc caggctcttt gccaggttcg cctgcaccag cgtgtcgccc
    18661 tttttcagcg gctcgccctc tttccagaag atagctttac tgttttcgtg attcggcaac
    18721 acttcgctac cgtaggtttt gagatcgtca gccagcgcgt cgttaacgat aaaaccatcg
    18781 cgtgccagtt taaacgcggg ctgcacgact ttgttcagcg gcatggtgcc gtatttatcc
    18841 agcgccagcg agaaacctgc taccgtaccc ggtgtgccgg aagccagatg cgaagtgagt
    18901 gattttttgc tgtccgggtt gccctgatca tcgaggaaca tatcgcgggt cgctttggcg
    18961 ggtgccattt cgcggaaatc gatagccgtg gtattgccat ttttcgagcg gattaacata
    19021 aaaccaccac cgcccagatt ccctgcctgc ggatgcgtta ccgccagcgc gtagcccacc
    19081 gccacggcgg catcaacggc attcccgccc tccttgagga tatccacccc cacctgagtg
    19141 gcagtggcgt ccacagacgc taccattccc tgtttcgcgc gtaccggatg gaagacatct
    19201 tcctccacac catacgagac gggcggcgca ggaggcgcgg cggcggcgct aaaacaactt
    19261 cctgagagca gagcagcaat ggccacccgg cgtaaaaacg tcggttttat catcgttatt
    19321 ctccagagat taaggggcaa ccccagctaa gcctggtata taactctgaa ttaatcatcg
    19381 ttttgtcggg aagcgagtaa acttaaagga tatcttcaga ggaggatacg cgatgaaacg
    19441 acttctgatt cttacggcac tcctgccgtt tgtcggcttt gcacagccca ttaatactct
    19501 gaacaaccct aaccagccgg ggtatcagat acccagccag cagcggatgc aaacccagat
    19561 gcagactcag caaatccagc aaaaagggat gctgaatcag caactgaaaa cgcaaactca
    19621 gttgcaacag cagcatttag aaaaccagat aaacaataat tctcagcggg tgttgcagtc
    19681 acagccgggg gagcgaaatc ccgcccggca gcaaatgctg cccaacacca acggcgggat
    19741 gttaaacagc aaccgtaatc cggatagttc gttgaatcag cagcatatgc tgccggagag
    19801 gagaaacggc gacatgctga atcagcccag cacgccgcag cctgacattc cgttgaaaac
    19861 tattgggccg taaagttcgg accaatcacg tcaatcgcat cggtacagat gcaatccacg
    19921 ccccagcgca gcaactctgc cgcgtgctgg ggtttattga cggtataaac cagaatccgt
    19981 aatccggcgt ctttcaactg catcactcgc gctttatcga gtaacttatg attgagatga
    20041 atagagacgc agcccagccg cgcggtcagt tcgcgccagt cgtcgcgcca ctcatcaagc
    20101 aataaaccac gcggcagttc cggtgccgcc tgttgtgcag cttctaaagc atcaatctca
    20161 aacgatgaca gcagcggcgg cgtcataccg gcccacagtt ggcgtgccgc cagcgccacc
    20221 attttgcccg ttaatggtcc agtgccagtg gtgggtttga tttcgatatt cgccatcatc
    20281 ccgtgttcgc gacagcgttc cgccacctgc gaaagcaacg gcagcggctc acctttaaac
    20341 gctttgctgt accaactgcc cgcatccacg cgcagtaaat cctgccagtt cagttcaccc
    20401 gcgacgcccc agccgttgct ggtgcgttcg agattgtcgt catggagcag gaagatctcg
    20461 ccatctttcg ataacttcgc gtcaaattcg atcatcttat gaccgtattt tgccccgacg
    20521 tcgattgccg ccagggtgtt ttccggggcc agcttaccgc cgccacgatg agcgacgatg
    20581 cggggataag gccagttact catactcgtt gtcctgtttc accatcaaaa agatgcagct
    20641 gattttccgc cagatgcagc cacagcgtgc tgcctgccgt cgggcgctcc tgatgcgcca
    20701 gtcgcaccac cagcttctgc ttgcgagctt agcgcaatat gttccgggcg aatgccgagg
    20761 gtcattttac gcccggcata ctgacggtag ccaccgttta gcggtagcac gataccgccg
    20821 tccagttcga aatgcgtgcc ttcattattc atgcggcctg tcagcaggtt catcgccggg
    20881 ctgccgataa aactcgccac aaacaggctg gcgggctttt cgtagacttc caccggcgtg
    20941 ccaatctgtt cggcaacgcc gccgttcatc accattactc gctgggcgag cgtcatcgcc
    21001 tcaacctgat cgtgagtaac gtagagtgaa gtcgttttca ggcgacggtg cagctgttgc
    21061 agttcaagac gcatctgtac gcgcagcttg gcatcgaggt tagagagtgg ctcatcaaac
    21121 aagaacaccg ccggatcgcg cacaatcgcg cgtcccatcg ccacacgctg gcgctgaccg
    21181 ccggaaagct cgcgcgggcg acgtttgagc agaccgtcca gctccagaat gcgcgccgct
    21241 tctttaacgc gctcggctat ttgctgcttg cccatgccgc gaattttcag cccccacgcc
    21301 atgttttctt cgacgctcat atgcggataa agcgcgtaat tctggaacac catcgcaatc
    21361 ccgcgatctt tcggctccat ttcggtcacg cgctggtcgt tgatccagat atcgccttcg
    21421 gtcaccctct ccagcccggc aaccatacgc agcagcgtcg atttcccgca gccagacggc
    21481 ccgaccatca cgataaattc gccatccgcc acatcaaggg tcagcggttt aatgacctag
    21541 gttttgccat cccagctttt ggttactgcc tgtaatttca gtcctgccat cttatttctc
    21601 actatcgacc aggccgcgca cgaaggcacg ctgcatcact aaaacaatca ctactggtgg
    21661 gatcagggtt aacaacatcg ccgccatcac tgagttccat tccgtggtgc cttcgccagt
    21721 ggcgatcatc cctttgatcc ctgccacggt ggtgccgaga tccacgtcgg taataatcaa
    21781 caacggccac aaatactggt tccagccgta aataaaggtg atcacaaaca gcgccgccag
    21841 attagttttg gagagcggaa aaacgatgtc gcaaaagaag cgcattggtg acgcaccgtc
    21901 gatccgcgcc gcttccacca gttcatccgg cagcgtcata aagaactggc gaaacagaaa
    21961 cgtggcggtc gccgaggcca tcagcggcag cgttaaaccg gcgtagctgt cgagcatctt
    22021 caggttggcg atgacttcca ccgtcgggaa gatacgtact tcaaccggca gcatcagggt
    22081 gataaaaatc atccagaaga agaggttacg tagcggaaaa cgaaaccaga caatggcaaa
    22141 tgccgagagc atcgagacag taattttgcc gagcgtaatg ctgaacgcca tcacaaagct
    22201 gttaagcaac atccgccaga acggcgcgct atttgtgcct accccgttca cccagatgtt
    22261 gtggatgttt tccagcagat gcgtgccggg gatgagcgtc atcggcgcgg cgtagacggc
    22321 ctgtttatcc agcgtcgccg cgacaaacgc cacgtacagc gggaagagga tcaccgcgat
    22381 cccgaggatc agcatggtat ggctgaatat cgtcagccac ggacggttct caatcattgg
    22441 taacgcacct tgctttcaac atagcggaac tgcaccaccg tcagcacgat gacgaggaac
    22501 atcagcacca tcgactgtgc ggcagacgaa gccagatcca gtccggtaaa accttcgcgg
    22561 tagatcttat aaatcagcgt cgtggtggcc tgaaccggcc cgccggacgt ggcggcgtcg
    22621 atcaccggga aggtgtcgaa gaaggcatac accaggttca ctaccagcag gaagaaactc
    22681 accggggcga taagcggcag cgcaatctta aagaagcggc gaatcggccc tgcaccgtcg
    22741 atggctgcgg cttcgatcaa cgaacgggga atggattgca gcgcggcata gaagaacaga
    22801 aagttgtagc tgatttgctt ccacaccgag gcaaacacca ccagaaacat cgcctgaccg
    22861 ctattttgcg cgtggttcca gtcgtagccg aactcagcga gaaaatgggt gatcagcccg
    22921 cgaccggggt taaaca
//
LOCUS       DADLTY010000053        22869 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.053, whole genome shotgun sequence.
ACCESSION   DADLTY010000053 DADLTY010000000
VERSION     DADLTY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.053"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..901
                     /locus_tag="JN334_001749"
     CDS             <1..901
                     /locus_tag="JN334_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706657.1"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. The beta-hairpin of the
                     Uvr-B subunit is inserted between the strands, where it
                     probes for the presence of a lesion; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HAY7437917.1"
                     /translation="RPLKFEEFEALAPQTIYVSATPGNYELEKSGGDVVDQVVRPTGL
                     LDPIIEVRPVATQVDDLLSEIRQRAAINERVLVTTLTKRMAEDLTEYLEEHGERVRYL
                     HSDIDTVERMEIIRDLRLGEFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSERS
                     LIQTIGRAARNVNGKAILYGDRSTPSMAKAIGETERRREKQQKYNEEHGITPQGLNKK
                     VVDILALGQNIAKTKAKGRGKSRPIVEPDNVPMDMLPKALQQKIHELEGLMMQHAQNL
                     EFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(1093..2001)
                     /gene="yvcK"
                     /locus_tag="JN334_001750"
     CDS             complement(1093..2001)
                     /gene="yvcK"
                     /locus_tag="JN334_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HAY7437918.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      2266..2410
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            2398..3387
                     /gene="moaA"
                     /locus_tag="JN334_001751"
     CDS             2398..3387
                     /gene="moaA"
                     /locus_tag="JN334_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAY7437919.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            3409..3921
                     /gene="moaB"
                     /locus_tag="JN334_001752"
     CDS             3409..3921
                     /gene="moaB"
                     /locus_tag="JN334_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HAY7437920.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            3924..4409
                     /gene="moaC"
                     /locus_tag="JN334_001753"
     CDS             3924..4409
                     /gene="moaC"
                     /locus_tag="JN334_001753"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAY7437921.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            4402..4647
                     /gene="moaD"
                     /locus_tag="JN334_001754"
     CDS             4402..4647
                     /gene="moaD"
                     /locus_tag="JN334_001754"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HAY7437922.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            4649..5101
                     /gene="moaE"
                     /locus_tag="JN334_001755"
     CDS             4649..5101
                     /gene="moaE"
                     /locus_tag="JN334_001755"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HAY7437923.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            5238..5942
                     /locus_tag="JN334_001756"
     CDS             5238..5942
                     /locus_tag="JN334_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY7437924.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            6147..6860
                     /locus_tag="JN334_001757"
     CDS             6147..6860
                     /locus_tag="JN334_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY7437925.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(6896..7852)
                     /locus_tag="JN334_001758"
     CDS             complement(6896..7852)
                     /locus_tag="JN334_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAY7437926.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(7852..9093)
                     /gene="clsB"
                     /locus_tag="JN334_001759"
     CDS             complement(7852..9093)
                     /gene="clsB"
                     /locus_tag="JN334_001759"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HAY7437927.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(9090..9851)
                     /locus_tag="JN334_001760"
     CDS             complement(9090..9851)
                     /locus_tag="JN334_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY7437928.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            9984..10394
                     /locus_tag="JN334_001761"
     CDS             9984..10394
                     /locus_tag="JN334_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437929.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(10356..11462)
                     /locus_tag="JN334_001762"
     CDS             complement(10356..11462)
                     /locus_tag="JN334_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7437930.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(11473..12606)
                     /locus_tag="JN334_001763"
     CDS             complement(11473..12606)
                     /locus_tag="JN334_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7437931.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(12599..14335)
                     /locus_tag="JN334_001764"
     CDS             complement(12599..14335)
                     /locus_tag="JN334_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7437932.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(14328..15326)
                     /gene="hlyD"
                     /locus_tag="JN334_001765"
     CDS             complement(14328..15326)
                     /gene="hlyD"
                     /locus_tag="JN334_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HAY7437933.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(15326..15997)
                     /gene="cecR"
                     /locus_tag="JN334_001766"
     CDS             complement(15326..15997)
                     /gene="cecR"
                     /locus_tag="JN334_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HAY7437934.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            16226..17413
                     /gene="rhlE"
                     /locus_tag="JN334_001767"
                     /pseudo
     CDS             16226..17413
                     /gene="rhlE"
                     /locus_tag="JN334_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(17620..18102)
                     /gene="ybiA"
                     /locus_tag="JN334_001768"
     CDS             complement(17620..18102)
                     /gene="ybiA"
                     /locus_tag="JN334_001768"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HAY7437935.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            18222..20372
                     /gene="dinG"
                     /locus_tag="JN334_001769"
     CDS             18222..20372
                     /gene="dinG"
                     /locus_tag="JN334_001769"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAY7437936.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            20400..21362
                     /gene="ybiB"
                     /locus_tag="JN334_001770"
     CDS             20400..21362
                     /gene="ybiB"
                     /locus_tag="JN334_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HAY7437937.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            21503..22588
                     /locus_tag="JN334_001771"
     CDS             21503..22588
                     /locus_tag="JN334_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HAY7437938.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
BASE COUNT         5413 a         5943 c         6151 g         5362 t
ORIGIN      
        1 ccgtccgctt aagtttgaag agttcgaagc attagcgccg caaaccatct atgtttcggc
       61 gacgccgggt aattacgagc tggaaaaatc cggcggcgat gtggtggatc aggtggtgcg
      121 tccaaccgga ttgcttgacc cgattatcga agtgcggccg gtggcgacac aggttgatga
      181 tcttctttcg gagattcgtc agcgagcggc aattaacgaa cgcgtgctgg tcaccacact
      241 gaccaagcga atggcggaag atcttaccga atatctcgaa gaacacggtg agcgcgtgcg
      301 ttatcttcac tcagatatcg acaccgtcga gcgtatggag attatccgcg acttgcgtct
      361 gggtgagttc gacgtattgg tagggatcaa cttactgcgt gaaggtctgg atatgccgga
      421 agtgtcgctg gtggcgatcc tcgacgctga caaagaaggc ttcctgcgtt ccgaacgttc
      481 gttgatccag accattggtc gtgcggcacg taacgttaac ggtaaagcga ttctctacgg
      541 cgataggagc actccatcaa tggcgaaagc gattggcgaa accgaacgtc gccgtgagaa
      601 acagcagaag tacaacgagg aacacggcat tacgccgcaa ggcttgaaca agaaagtggt
      661 cgatatcctg gcgctggggc agaacattgc caaaaccaaa gcgaagggca gaggaaaatc
      721 gcgcccgatt gttgagccgg ataatgtgcc gatggatatg ttgccgaaag cgttgcagca
      781 gaaaatccat gaactggaag ggctgatgat gcaacacgcg cagaatctgg agttcgaaga
      841 agcggcacaa attcgtgacc agttgcatca gttgcgtgag ttgtttattg ccgcatcgta
      901 acaggatagc gaagaagact gatgacaaac ggaaaacagt ctgatgcgct acgcttatca
      961 ggcctacatt ttctccgcaa tatattgaat ttgcgcggtt tgtaggccgg ataaggcgtt
     1021 cacgccgcat ccggcataaa caacgcgcac tttgtcttca ataggaagcc ggaattttcc
     1081 ttcggatttc cgttaaccta aagcctgtaa cgccttttcc agcgcgttat gtaacaactg
     1141 gcggtcatga cgatacggaa tatcgctggc ctccagtacc tcctggatca caatccgctc
     1201 tttcaccgcc gagacatcca cttttggccc gacgatgacc gcatcaatga cctttttacc
     1261 aacatactgc tccataattg ccagcttgct ttccagcttc aaattagccg caggtaaact
     1321 caactcacgc cccagattgc cgatataaac catcggcgct ggcgtgcggc gtaatgcctg
     1381 ggcgatttcc ttcagcagca gaattggcat caggctggta taaaaactgc caggcccaat
     1441 aatgatgaga tccgcttcat tgatagcgtg aaccgcctca cgcgttgcgg gtacattagg
     1501 cgttaacaat aactcttgaa tcggcgtagt taactggtcg atattgacct cgccgtaaac
     1561 ttcatgcccc tgatcgtcaa tcgccatcag atcaacagga tgctctgaca ttggaatcaa
     1621 atgcgtatcc actttcagca gattacgaat taaattgatg gcttccagag gccgcacgct
     1681 aaggtgatcc agcgccttta acatcaagtt tccgagatta tgaccggaaa gttcgccatt
     1741 gccgccaaaa cggtattcaa acatcgcgga ggcgacgttc ggttccgtta tcagctggtt
     1801 gaggcagttg cgcatatcgc cccaagcaat gccgccttct gaacggcgaa tacgccccgt
     1861 cgagccacca ttatccgtgg tggtgacgat acccgttaaa cgagaaccca aagacgaaag
     1921 tgatgagaga acgcgtccca gtccatgccc tccgccgaga gcaacgacac gatcaagatc
     1981 agccagcgta cgattgcgca tatagtttcc tggagtcaga ttatccgcgc tacagtagcg
     2041 caaattgcgg ggaaacagca attaacctgc caatattgag gatacaaact attttcttct
     2101 atctctaaag gacgatgcac gctatgcctc cctgatgatg tatatcaaag taaaaccgcc
     2161 attttccctt attctgtagc gaaatagcac gatcatgacg ctatatacat gattacatag
     2221 cgaaagtgtg gatggtaaaa atctcattta cacgctagta tcggcttaac cactaaacac
     2281 tctagcctct gcacctgggt caactgatac ggtgctttgg ccgtgacaat gctcgtaaag
     2341 attgccacca gggcgaagga agaaatgact tcgcctcccg tatctggaaa ggtgtacatg
     2401 gcttcacaac tgactgatgt atttgcgcgt aagttttact acttgcgcct gtcgattacc
     2461 gatgtgtgta actttcgttg cacctactgc ctgccggatg gctacaaacc gagcggcgtc
     2521 accaataaag gctttcttac cgtcgatgaa attcgccggg ttacgcgcgc cttcgccagt
     2581 ctgggcaccg aaaaagtgcg cctgacagga ggagagccgt ctttacgccg cgactttacc
     2641 gatatcatcg ccgctgtgcg ggaaaacgac gctatccgcc agattgcggt caccaccaat
     2701 ggttaccgtc tggaacgcga tgtggcgaac tggcgcgatg cgggacttac tggcattaac
     2761 gtcagtgtcg acagtttgga cgcccgccag tttcacgcta ttaccgggca ggataaattc
     2821 aaccaggtca tggcagggat tgatgctgca tttgaggccg gttttgagaa ggtcaaagtc
     2881 aataccgtgc tgatgcgtga tgttaatcat caccagctcg acacctttct gaactggatc
     2941 cagcatcgcc ctatccagct gcgtttcatc gaactgatgg aaacgggcga gggcagcgag
     3001 ctcttccgta agcatcacat ctctggtcag gttctgcgtg acgagctact gcgtcgcggc
     3061 tggatccacc aattacgtca acgcagcgac ggtcccgcgc aagtcttttg ccatccagat
     3121 tacgccggag agattggcct tatcatgccg tatgaaaaag acttctgcgc cacttgcaac
     3181 cgcctgcgcg tttcctccat tggtaaactc catctctgcc tgtttggtga aggcggcgtt
     3241 aacctgcgcg atctgctgga aggcgatacc cagcaacagg cgctggaagc gcgtatttca
     3301 gcggcgctgc gggagaagaa acagacccat ttcctgcatc aaaacaacac cggtattacg
     3361 caaaacttat cgtacattgg cggctaaaac gtcaaaagga gagatcagat gagtcaggta
     3421 agcactgaat ttatcccgac ccgtattgct attcttacgg tttctaatcg tcgcggtgaa
     3481 gaagacgata cctccggtca ctatctgcgc gattcggcgc aagaagcggg ccatcacgtt
     3541 gtcgatgaag ccattgtgaa agaaaaccgc tacgctattc gcgctcaggt atctgcgtgg
     3601 atcgccagcg acgatgtaca agtggtattg attacgggtg gtactggcct gacggaaggt
     3661 gatcaggctc ccgaagcgct gctgccgttg ttcgaccgtg aggttgaagg ttttggtgaa
     3721 gtgttccgta tgttgtcgtt tgaagagatt ggcacttcca cgttgcaatc tcgtgcggta
     3781 gcgggcgtcg ctaacaaaac gctgattttc gccatgccgg gttcgaccaa agcgtgccgt
     3841 accgcatggg aaaatatcat cgcgccgcag ctggatgccc gtacgcgtcc gtgtaatttc
     3901 catccacatt tgaagaaata agtatgtcgc aactgaccca tatcaacgcc gctggcgaag
     3961 cgcacatggt ggatgtctcc gccaaagcgg aaaccgtgcg tgaagcgcgg gcggaagcct
     4021 ttgtcaccat gcgcagcgag acgctggcga tgattattga tggtcgccac cacaaaggcg
     4081 acgtatttgc cactgcgcgt attgccggta ttcaggcggc aaaacgcact tgggatctga
     4141 tcccgctgtg tcacccgctg atgctcagca aagttgaagt caatttacag gccgagccgg
     4201 agcacaatcg ggtgcgtata gaaaccttat gccgcctgac cgggaaaacc ggtgtcgaaa
     4261 tggaagcgtt aaccgcggcc tccgtggcgg cgctgaccat ttatgacatg tgcaaagcgg
     4321 tgcaaaaaga tatggtgatt ggtccggtac gtttgctggc gaagagcggc ggcaagtcgg
     4381 gtgactttaa ggtggaagcg gatgattaaa gttctttttt tcgcccaggt gcgcgagttg
     4441 gtgggaacag atgcaaccga agtggctgcg gattacccaa ccgttgaagc gttacgccag
     4501 cacctggctg cgcagagcga tcgctgggcg ctggcgctgg aagatggcaa attgctggct
     4561 gccgtcaacc agacgctggt gagttttgac catccgctga ctgacggcga cgaagtagct
     4621 ttcttcccgc cggtaaccgg aggttaagat ggcagaaacc aaaattgttg tcggcccgca
     4681 gccgttcagc gtaggagaag agtacccgtg gctggcggag cgtgacgaag acggtgcggt
     4741 agtcaccttc accggaaagg tgcgcaacca taacctgggc gacagcgtca aagcattaac
     4801 cctcgaacac tatccgggga tgactgaaaa agcactggca gaaattgttg atgaagcgcg
     4861 taaccgctgg ccgctggggc gcgtcacggt gattcaccgc atcggggaat tatggccggg
     4921 cgatgaaatc gtttttgtcg gtgtcaccag tgcgcatcgc agcagtgcgt ttgaagccgg
     4981 gcagtttatt atggattatc tcaaaacccg cgcaccgttc tggaagcgcg aagccacgcc
     5041 ggaaggcgac cgctgggttg aagcccggga gagcgatctg caggcggcaa aacgctggta
     5101 gttttttgtt agccggataa ggcaccgctg cgtccggcaa tttcaccgct cagtacatgc
     5161 gtttacgctg cgtcgatgca cagcctcatc attttgcagt atccttaaga tattccttat
     5221 atcttcagga gatcgtcatg gacagattcc cacgttctga ttcaatcgta caaccctggg
     5281 ctggcttgca aacttatatg gcgcaagtct atggctggat gaccgttggc ttgttgctga
     5341 ccgcatttgt tgcctggtat gcggctaatt ccgcggccgt gatggagctg ttgttcacta
     5401 accgtgtctt tttaatcggt ctgatcatcg cgcaattagc attggttatt gtgttatcag
     5461 cgatgattca aaagctgagc gcaggtgtaa cgacgatgct ctttatgctt tattcggcgc
     5521 tgacgggtct tacgctttcc agtatattca ttgtctatac cgctgcttct atcgccagta
     5581 ctttcgtcgt tactgccggg atgttcggcg caatgagcct gtacggttac accacgaagc
     5641 gcgatttaag tggctttggc aatatgctgt ttatggcgtt aatcggcatt gtgctggcat
     5701 cgctggtcaa cttctggctg aaaagcgaag cattgatgtg ggcagttacc tacatcggcg
     5761 tgattgtctt tgtcggattg acggcgtatg acactcagaa attgaaaaat atgggtgagc
     5821 agattgatac ccgcgacacg tcgaacctgc gcaaatattc cattcttggt gcgttaacct
     5881 tgtatctgga cttcatcaac ctgttcctga tgttgttacg gatcttcggc aaccgccgtt
     5941 aattcttttt caccatcact atcccggcca tgtgtcggga tttttttctt actaaaaacg
     6001 acacttaaca agtaccaggt ataaaatatg ccttatgagc gagcggaatc gttttccgta
     6061 tccataaata caaaagtatt ttgtatgcgt ggttttaaat ttattgatgg tgaatattaa
     6121 tgttttttct aaggacaact aaaagaatgg aatcatactc gcaaaacagc aataaattag
     6181 attttcagca cgaggccagg atattaaacg gtatatggct cattaccgct ttaggtttgg
     6241 tggcaaccgc aggactagcc tggggagcta agtatctcga aattacggca accaaatatg
     6301 attcaccacc aatgtatgtc gccatagggt tattattact ttgtatgtat ggcttaagta
     6361 aggatatcaa caagataaat gccgccatcg cgggcgtaat atatctgttt ttactctctt
     6421 tggtggcgat tgtcgttgca agtttagttc ctgtatatgc cattatcatc gtgttcagca
     6481 ctgcgggcgc gatgttttta atcagtatgc tggccggttt attatttaat gttgatcctg
     6541 gttctcaccg ttttatcatt atgatgacgt tgacagggtt ggccctggta atcatcgtga
     6601 atgcggcatt aatgagtgaa cggcccattt gggtaataag ttgcttaatg attgtgttat
     6661 ggccaggcat tatctcgcat ggacgaaata agctccttga actggcagga aaatgccata
     6721 gtgaagagtt gtggagtccg gttcgttgcg cttttacagg tgcattaaca ctctattact
     6781 attttatcgg cttctttggg atacttgccg cgatagctat aacgcttgtc tggcaaaggc
     6841 atacgcgttt ttttcattag tcgtaaatgc agatactaaa gaacttaact aaacttcaca
     6901 tcgccgcttc atttttcgcc cgtagcttct tcgcttggct ttccagtagc aggtaacaga
     6961 tcagcgccag cagcagcggg ataaagtaat acaggacgcg ataagcgagc agggcggcga
     7021 taatgctccc tttcgaggta tgctccccag ccagtagtgc gataaacacc gcttccagca
     7081 caccgatccc cgccggaata tgcacgatga cgccagcaat actactaacc agtaacacgc
     7141 ccagtacaaa gaaatagttc acgctttgac caagtaacag ccagataatc gcccccatta
     7201 ccatccagtt aacgctggaa atcagcatct gagcgagggc gaatttccat gaaggcagca
     7261 ccagcttttg ccctttgatg gtcatatggc ggtgcttcgc gaaagcgcaa aaccacaaat
     7321 aaaccgcgat aatcatcagt aagccaatgc cgagaatacg cagcgtagtt tgatcgacat
     7381 accagtaatc cggcaactcc accacgcctg cggtaaagat aatccctgcc agtaaaatat
     7441 agcccagcca gttggtggta atactgagcg aggaaatccg cgtaatagtg ctgcccggta
     7501 accctagtcg ggagtacaaa cgatagcgca taccaatgcc gccgacccag gtactgagcg
     7561 tcaggttgaa ggcgtagcag ataaacgaca ccagcatcac ctggcgcttc gccagtttgt
     7621 gaccgcagta aaagcgggcg agcaggtcat agcagccgta aatcagatag ctgacgacca
     7681 ccagcccgac cgcactaagc agcgcaacgc gattgtagtc gcggatgacc ttccagacct
     7741 cttcccagtc cacttttttg gcgtagacca ccagcaacac aatcaccgcg ataaaaaaca
     7801 gccaggtgag gatcttcttt gctaagcgcc agcgcgggtg tgatttactc atcaggggtt
     7861 tacccccgtg ttttcagttt ctacccgatc ctgcgtttcc attgtcggtt gtgcgggggg
     7921 atcaacctgc gtcagacgtg gtgtgtgtgc cggaagccag ccaaccagcg ccgggaagtg
     7981 gcgtaaaaag tggaacgcca gcacgctttt ggtcaggttc caccaggtgc gtttgggcag
     8041 catggtttca tccacctgct gacaatctgc ggcaataatg ccgttcagat tatcgcgcag
     8101 cgtctggtta aaatgacgat cgtggatgat gacatttgct tcgagattca gtgacaaact
     8161 gagcggatcg agattactgg accctactgt cgcccagtga tcgtccatca atgccacttt
     8221 gccgtggagc gggcggcggc ggtactcaaa aacctgaacg ccgcctttaa ccagatagtt
     8281 atacagcaag cgcgcaccga ctctgacaat cggcatatcc ggttcgccct gaatgatcag
     8341 tttgatccgc accccgcgcc gtgccgcttt acgcaaggcg tgtaaaaatc gatagccggg
     8401 gaagaagtag gcgttggcga taatcacttc ccgctgcgcc tgagtgagca ttttcagata
     8461 atggcgttca atatcatcgc gatgttcttc gttatcgcgc cagaccagca atacctgcgc
     8521 ttctcccggc tggcggttct cttccgcttt gtgatgacgt cgccaccagc gtcgtgccgc
     8581 gctctgtcca ggcaggtttt ccagctcaaa ctggagaata tcttcgacaa tcggcccttc
     8641 aaggcgtacc gcgtaatcct gtttagcctc tggaccgtag ctggacatat gctcggcgga
     8701 gtaattcagc ccgccaataa aggcgatacg cgcgtcgatc accacaattt tgcgatgcat
     8761 ccggcgaaac acattggtgc gcataccaaa aaggcgaggg cggggatcgt agtagcggaa
     8821 cactacgcca gctgccgtca gttcattgac aaactcatcg ctgagatccg gcgaaccgta
     8881 gccatccagc aagacttccg ctttaacccc gcgttgcgct gctgccagta gtgccgcatg
     8941 cagctgtttg ccgacgttat cctcaaacca gataaacgtt tcaagaatga tgcgttcttg
     9001 tgcctcgcca atcgccttaa acaccgcggg ataatattgc tcgccgtttt ccagcaactg
     9061 gatcttattg ccttcgcgcc agctacattt cataaatgaa tctccgcact taaaggggca
     9121 tgatcagaaa ggtgtcgcca tgtccgcagc ggcaacgcgg ttggcgcgct ggcgctggca
     9181 tttttgacgt agatcctgtc cagtcgtagt agaggaaatt gcaccggaaa cgtgcgcgcc
     9241 gggcgtccgt gggcgcgggt aaaaatctca tccagtccgg cctgcacttt taacggatga
     9301 ttagcttttt gccgccagtc attgaaatca cccgccacca ataccggttc gccgtccggt
     9361 agctcattca cccattcggc gagcatcgca agctgcgcct gacggtgcgc ctcacgcagg
     9421 cccagatgta cgcacatcac atgaatcgct tttccggtca tcggcggcac aatgcggcag
     9481 tagagcacgc cgcgcttttc cgcaccatcg accgaaacat cgcgattctc ataatgttca
     9541 atgggataac gcgacagtac ggcgttgccg tgatgccctt ccgggtatac ggcattgcga
     9601 ccgtaggcaa aatcgctcca catagtgtcg gcgagaaact cgtagtgcga ggtatcgggc
     9661 cagttttcca catgcagcgg atgaacttcg tgcgcgccca tcacttcctg caggcaaaca
     9721 atatcggcgc tgacggtacg cacggcatcg cgaagttccg gcaaaatgaa gcgtcggtta
     9781 aacgcggtaa agcctttgtg aatgttgatg gtgagcacct tgaacgaaaa ttgttgtgtt
     9841 tgatcgggca taattttcct gtctttgcct cttatctcat tgaaatagtg tagtcggcgt
     9901 cacaaaaagg tgcggtctta cggaattttc cgtaaagttc ggtactctga gtaagtagag
     9961 ataaattctt caggagagaa gccatgaagt ggcaacaacg tgttcgtgtc gcaacgggtc
    10021 taagttgctg gcagattatg ttgcatttac tggtagtggc gctgctggtg gtgggctgga
    10081 tgagtaagac tctggttcac gtcggcgtgg gattatgcgc actgtattgt gtcacggtag
    10141 tgatgatgct ggtgtttcag cgccaccccg agcaacgctg gcgtgaggtg gcagacgtgc
    10201 tggaagagct gaccacgacc tggtattttg gcgcagcgct gattgtgctg tggctgttgt
    10261 cccgcgtact ggaaaacaac tttttgctgg caattgcagg gctggcgatc cttgctggcc
    10321 cggcggtagt gtctttgctg gcgaaagata agaagttaca tcaccttacg tctaaacatc
    10381 gcgtacgccg ctgaccctgt cgtggccgtt atcaccagta gcggccacaa actattccac
    10441 acaatatcca gactcgcatc cttcaaataa atctgcttgg taatgtccgt aaagtggcga
    10501 atagggttaa tccacgtcag attttgcagc cacactggca tgttttccac cggcgaaacg
    10561 taaccggaaa ggagaatggc gggcatcata aaaacaaaca cgccgataaa cgcctgctgt
    10621 tgtgtcgagc agagtgatga aatcaacaga ccgaatccca ccagcgataa accgtaaatc
    10681 accatcgtaa agtagaacag cgctagcgat ccggcgaagg ggatttgata cgcccagata
    10741 ccaatcgcca gcacaatggt ggcctggaag gtggcgacaa ttaacgccgg tacggctttg
    10801 ccgatgaaga tctgccaggt ggtgagcggc gaaaccagta gctgatcgag cgtaccttgt
    10861 tcacgttcgc gggcgacgga aagtgaagtg acgatcatta cgccgatagt ggtgatcatg
    10921 gcgatcagcg acggcaccac aaaccatttg tagtcgagat tcgggttata ccagttgcgt
    10981 accaccagct cgctgttgtt gggtttcggt tttccttcca gcagctcctg ctgataattt
    11041 ttgacgatct gttgcaggta gttggcggca atttgcgcac tgttggagtt acgcccgtcg
    11101 aggatcaact gcaacggcgc ggtctggaag gtatccagtt tgcgcgagaa gtcagccggg
    11161 aaacgcacca gtagtaacgc cttttgtgtg tcgatggtcg ggcggatctc ctgtgggctt
    11221 ttcagcagca gcacatgtgt aaaggcgctg gcgcgggcaa aacgttgagt aagttccacc
    11281 gaatgctcgc cgttatcttc atcgtagatg gcgatggtgg cgttagtcac ttccagcgtg
    11341 gcggcgaacg ggaacaggat cacctgaatt agcacgggta aaatcagaat cgcgcgggtt
    11401 tgtggttcgc gcagcaacga ctgcaactct ttgcggatta acgtccataa gcgatgaaac
    11461 atgctcttct ccctaatcca gccgacgttt ggttttcagc cacgtcaggc cgataaacat
    11521 caccgccgaa gcgatcaaaa acagcacgtt taccaccagc accactggaa tattcccggc
    11581 gaggaacagg ctttgcaggg tgctgacgaa ataacgagcg ggaataatgt acgtcaccgc
    11641 gcggatcacc gcgggcatac tgtcgatctg aaaaataaag ccggaaagca taatcgacgg
    11701 cagaaaagcg gcgttcaggg cgacctgagc ggcattgaac tggttgcggg taatcgtgga
    11761 aatcagcagc cccattccca gggtactgag taaaaacagg ctggagataa aaaacagaat
    11821 cagcagcgaa ccgcgatacg gcacgccgag aataaacact gacaccagca tacacagcaa
    11881 catcgccagc atcccgagaa agtaataagg gatcagctta cacagcagca gttccgtgcg
    11941 ggtaatctcc gtagagagca gagcctccat ggtgccgcgt tcccattctc gcgccaccac
    12001 cagcgaggtg agaatcgcgc cgatgaccgt catgataatg gtcaccgcac ccgggataat
    12061 aaagtgctgg cttatcgccg ccgggttaaa ccagtagcgg gtttgtacat caataagcgg
    12121 ttcaaaagtc tgcccgttgt cctccgctcg ctgcatttgc cagatctgcc agatcccttc
    12181 gacatacccc tgcacaaagt tagcggtatt cggctcactg ccgtcggtga tcacctgaat
    12241 cggtgcggtg gcgttggcgc gctccatctg ttcggcgaaa tccacgggaa taaccaccag
    12301 accgcgaatt ttccccgact gcattttggc gatcagttcc tgacggttat cgctgatggt
    12361 ggcgtcgatg tagggcgaac cggtcatggt gtgggtgaaa tccagcgccg cttcgctacg
    12421 ctgttccagc aaaatcccga cccgcagctt gctggagtcg aggttaatgc cgtaaccaaa
    12481 aataaacagc agtagcagtg ggatcactac cgcaatcagc cagctactcg gatcgcgaac
    12541 gatctgccgc gtctctttga cgcacagcgc ccgcacgcga tgccaggaaa ggatcgggtt
    12601 actcattgct atgctcctta tcccagtcgt ggatcaactg aataaaggct tgctccatgg
    12661 tgggatcggg ttgctcatcg ttagctgact gtgctttcaa atcgtccggc gtaccgctgg
    12721 cgattaattt cccacggtac accaggccga tgcggtcgca atattccgct tcatccataa
    12781 agtgggtggt gaccatcacc gtgacgcctt tctctaccat gctgttgatg tgcagccaaa
    12841 attcacggcg ggtgaggggg tcaacgccgg aagtcggttc gtcgagaaag agaatatccg
    12901 gttcatgcat cagcgaacag gccagcgcca gccgctgttt aaaacctaat ggcagttcat
    12961 cggtggcgtg tgaggcaata cttttcaggc caaatgcctc gctcatacgg gagatttttt
    13021 cgttctgcgc ccgaccgcgt aagccataca caccagagaa aaagcgtaaa ttctgttcga
    13081 ccgtcaggtt gccgtagagc gaaaattttt gcgccatata gccgagatgc tggcgcgctt
    13141 taccggaact ctctttcaga tccatcccca gcaccagcgc ctggccggaa gtcggcacca
    13201 gcaaaccgca catcatctta aaggtggtcg atttacccgc gccgtttggc cccagcaaac
    13261 caaaaatctc cccacgttta acggcaaagt tgacgtgatc ggtggcggca aaatcaccaa
    13321 atttcttggt cagttctttc gcttcgatca ccgtctcgcc gggtgtgcct tctaccgtat
    13381 gcaatattgc acccagcggc gattctgagg ttccggcacc gcccagcaaa tcaataaacg
    13441 catcttcaaa acgcggcgta gtttcgttga tgttgatttc cggcatcccg tcggcatggc
    13501 gaatatcgtc tggtgtggcc tcttttttga ggatcagacg taccgatttc ccctgaatca
    13561 tgccatcgct gacctgcggc agtttcaagg cgcgttgcaa cagtttgcgg ttgccctcgt
    13621 gtggactggt catcagaaag ctgcgtccgg ccatggtttg tgtcagggct tttggttctc
    13681 cctgatacag caactcgcct tcgttcatca gtaacacgtc acggcactgc tcggcttcgt
    13741 cgagatacga ggtactccag aggattaaca tcccttcgcc cgccagctca tgcaccatct
    13801 gccacagttc gcgccgtgag atagggtcaa cgccgacgcc gggttcatcg agcagcaaca
    13861 ctttcggttc gcccaccagg gtacaggcca ggccgagttt ttgtttcatc ccaccggaga
    13921 gcttgcccgc caggcgtccg gtaaacggcc caagagacgt aaactccagc aggcgagcaa
    13981 aagtttgctt acgtgcctcg ccggtgacgc tgcgcaaatc cgcgtacaga ttgaggttct
    14041 ccatcaccgt gagatcttca tacagaccaa atttctgcgg catatagccg agcacggcgt
    14101 gcagcgcgcc gtcgtttttg atcggatcaa agccaatcac cgtggcactg ccgctgtcgg
    14161 gtttcagtaa tcccgccaac atccgcatca gtgtggtttt acctgcaccg tccggcccca
    14221 ccaaccccgt cacataaccg gcgtgaatgg tacaatcgag cggcgcgacg gcgggcttgt
    14281 ccatgcccgg aaagcgtttt tccaggccgt tcagcgtgat aacggcatca ttcatgtcct
    14341 gcctcgtcac cgaattgtac cgtcactggc attccctggc gtaacgcatc atcggcgtcg
    14401 gtcaccacaa tacgcaggcg atagacgagg tcggtacgca gatccggtgt ttcgacggtt
    14461 ttcggggtaa attcagcagt cggcgaaacg aaaccaatct gcccgtgata cggcttgtcc
    14521 gggcgaccat cggtataaag cagcactttg cgccccggct gggcctggtc aagattccgt
    14581 tcatcaacat aagcgcgcac ccacaccgga cgcgttagtg aaacggtaaa caccgtgcca
    14641 ccttcattga gaaccgtgcc tggctctacc gcgcgcgtta acagcgtgcc atcagacggt
    14701 gctatcaacg ttgagtcctg taaattcagc tccgcctgcg ccagttgcgc ctgcgcctgt
    14761 tcgaggctgg ctttcgcctg agcgatgtcc tgttcacggt taccggaacg gtactgacgc
    14821 aatttatcct gtgctgattt cagcgttgcc tgtgcctggt cgcgcgagga gcgggcattt
    14881 tccaggtcat ttgccgaaat ggtgcggctt ttccacagtc cttgctggcg gttatagaag
    14941 ttctgcgcat agtcataggc ggcttgcgcc tgtttcaccg ctgcggcggc ctgagcgatt
    15001 tcttcatcgc gatacccggc aagcatcaga tcatactgcg cctgtgccac cgagacgccc
    15061 gctttcgcct gcatcagggc aatctcatac ggcttgtgat ccagttcgcc cagcacctgg
    15121 cccgctttga tagcatcacc ttcgtccacc gccagcgatt caacgcgccc cccaacacgg
    15181 aaactaagat tcaccgtacg aatatccacg ttgccataca gcgtcaggcc gttatcctgg
    15241 cggctttgat accaccagta gcctccggca accacggcgg caagtaccac taccgccagt
    15301 ccgatcacga caggtttttt catcactaca aactcctttg cgataatcct tgcaaaatca
    15361 ggtcgatatg acaggtcacc gtctggttga tcagttcggt cttttcttca tcgaacgcgg
    15421 tccagccggt acgtaacaga attgtttctt tgccaagacg gaacgccaga atctcaccaa
    15481 tcaacgcatg ggtatgaagg atcatgcggg tgtcattagc gtcgcagccg gtccaggcgg
    15541 caatcaggcg tgtcaggtgg ctgtgtagcg gactaatcac ctgttcgtgc accaggtggt
    15601 aggctgccgt gggagagagc tgctcacggg agataaactt gctgaggttg acggtgtcat
    15661 cctgggtgag cagcttaatc atgttcctgc aggcgcgaag gatcagttca cggatggcag
    15721 cccgatcagg ctgtggttgg gcgaacaagc gttcggcttc ctcggcatgc ggacggaact
    15781 gctcgccaat aaaatcggca atccactggg cgcaggcgag gtacaaatct tctttcgaac
    15841 cgaagtagta ggtgatggca gcgatattct gcccggcctg ggcggctatc tcgcgggtgg
    15901 tggcgttcat tccatattca ccaaactgcg ccagtgcggc agcaatcagc tgttttttcg
    15961 cctgttcacc cttgattgtc atggcaggat tattcatcgc acagtccatt cttaatcaaa
    16021 tgattgatta agattatgac tccatatggg agttgtccag tatggctaag aattttagct
    16081 acgccagtca cagggataat ttatgcgctg cgtcacaaaa actgctacac tccgctccct
    16141 catgacattg tggtttttgt cattttcctt ttcagtatct ccctgaaaac tacaccggta
    16201 acggtcgggg cggttcggag tagttatgtc tttcgattct ttgggtttaa gccctgatat
    16261 cctgcgcgcc gttgccgagc agggttaccg tgaacccacc cctattcagc agcaggcgat
    16321 ccctgctgtg ctggaaggcc gcgacctgat ggctagcgcc cagaccggca ccggcaaaac
    16381 agcgggcttt acgctgccgc tgttgcaaca cctgatcact cgccagccgc acgccaaagg
    16441 gcgtcgtccg gtacgtgcgc tcattcttac cccgacccgt gaactggcgg cacagattgg
    16501 cgaaaacgtc cgtgattaca gcaaatacct gaacattcgt tcgctggtgg tgtttggcgg
    16561 tgtcagcatt aacccacaaa tgatgaagtt gcgcggcggc gttgatgtgc tggtggcaac
    16621 gccgggacgt ttgctggacc tggaacatca aaatgcagtg aagctggatc aggttgaaat
    16681 cctcgtcctc gatgaagctg accgcatgct cgacatgggc tttatccacg atatccgtcg
    16741 cgtgttaaca aaactgccag cgaaacgcca gaacctgctg ttctctgcga ccttctctga
    16801 cgatattaaa gccctggcgg aaaaactgtt gcacaacccg ctggaaatcg aagtggcacg
    16861 ccgcaatacc gcgtctgatc aggtgaccca gcacgttcac tttgtcgata agaaacgcaa
    16921 acgcgaattg ctgtcgcaca tgattggtaa agggaactgg cagcaggtgc tggtgtttac
    16981 ccgtaccaaa cacggcgcta accatctggc tgagcagctc aataaagatg gcatccgtag
    17041 tgtggcgatc cacgggaata aatcgcaagg tgcgcgtacc cgtgcgctgg ctgattttaa
    17101 atcgggtgac attcgcgtac tggtggcgac cgatatcgct gcgcgcggtc tggatatcga
    17161 agagttgccg cacgtcgtca actatgaact gccaaacgta ccggaagatt atgtccaccg
    17221 tatcgggcgt accggtcgtg cggctgctac cggtgaagcg ttgtcgctgg tgtgtgttga
    17281 tgaacacaaa ctgctgcgtg atatcgaaaa actgctgaaa aaagagatcc cgcgcattgc
    17341 gattccgggc tatgagccgg acccgtcaat caaagccgaa ccgatccaga acggtcgcca
    17401 gcaacgtggc ggcatcaggc tgatgaacaa acgcaaaact gcctgatgcg ctacgcttat
    17461 caggcctacg tgaactctgc aatatattga atttgcatgc ttttgtaggc cggataaggc
    17521 gtttacgccg catccggcat ttcacaacaa gcacttgtca gcaatttgag aacacgggaa
    17581 aaaatttatc tgttttaccg cccataagcc accgagacgt tacttctcta tagccaattg
    17641 ttcgcgcaac tccattaaaa ggtagcccag tcgattcttg cccttaccat taccaccgtc
    17701 tccccagtaa gcatcgtttt ccgtatgctc aaccagtttt gcgggcgcgg ttgccagcaa
    17761 gagcacacgc agttctgcat gctgttcgaa tttagcgcga agtgctttac gcatcacctg
    17821 ttctttaacc gactcccagt ttttacgaag tggcttggaa cgattacgcc ccatgcgagc
    17881 ggcgaccatg ggggaagaaa cccgacggat ctcttcgcgg tatttttcat caaggaattt
    17941 ttgtgcctga aaatagtgtt cggaggtggg ccaggtattc ccgtcaacct tgatgggcct
    18001 ggcggcaaag ttagaaaaat caccgtagtc atcgctggtg ctgtagaaat ttatgatggt
    18061 gtcttgcatg acgtgttgga tcctttgtgc tcgaacgggc attaaaccgc attatgttgg
    18121 tggttattgc gagccgcttt ccagaaacag aaaaaccatt acccctgaaa accgaaaaat
    18181 gccacaatat tggctgttta tacagtattt caggttttct catggcatta accgccgcgc
    18241 ttaaagcgca aattgccgcc tggtataagg cgcttcagga acagatcccc gactttattc
    18301 cccgtgcgcc gcagcggcag atgattgcgg acgtcgccaa aacgctggcc ggagaagaag
    18361 ggcggcatct ggcgattgaa gcccccaccg gcgttgggaa aacgctctcc tatttgatcc
    18421 ccggcatcgc cattgcccgc gaagagcaaa aaacgctggt ggtgagtacc gccaacgtgg
    18481 cgttgcagga tcagatttac agcaaagatt taccgctgct gaaaaagatc attcccgatc
    18541 ttaaattcac tgccgctttc gggcgtgggc gctacgtttg tccacgtaat ctgacggcgc
    18601 tcgccagcac tgaacccacg caacaggatc tgctggcgtt tcttgacgac gaactgacgc
    18661 caaataatca ggaagagcaa aaacgttgtg cgaagctgaa gggcgatctc gacacttata
    18721 aatgggatgg tctgcgcgat catacggata tcgctataga tgatgatctc tggcgtcgtt
    18781 tgagtaccga caaagccagc tgcctcaacc gtaactgtta ctactatcgt gaatgcccgt
    18841 tttttgttac tcgtcgggag attcaggaag cggaagtggt ggtggcaaac catgcgctgg
    18901 tgatggcggc gatggaaagc gaagccgtac tgcctgaccc gaaaaatctg ctgctggtgc
    18961 tggacgaagg tcatcacctg ccagatgtgg cccgggatgc gctggagatg agcgctgaaa
    19021 tcaccgtgcc ctggtatcga ctacagctgg acttgttcac gaaactggtc gctacctgca
    19081 tggagcagtt tcgcccgaag accatcccac cgctggcgat ccctgaacgt ttgaatgcgc
    19141 attgtgaaga gttgtatgag cttatcgcct cgttaaacaa cattctcaat ctctacatgc
    19201 ctgccgggca ggaggcagag caccgttttg cgatgggcga actgcctgat gaattgctgg
    19261 agatctgcca gcggctggca aaactcaccg agatgctgcg tggtctggcg gagttatttc
    19321 ttaacgattt aagtgagaaa accggcagcc acgatattgt gcgtctgcat cggttgattt
    19381 tacagatgaa ccgcgcgctg gggatgtttg aggtgcaaag caaactctgg cggctggcgt
    19441 ctctggcgca atcttccggt gcgccggtga ccaaatgggc gacgcgggaa gagcgcgaag
    19501 ggcagctaca cctctggttt cactgcgtgg gaatacgtgt cagcgatcag ctggaaaggc
    19561 tgctgtggcg cagtattccg cacattattg tcacctccgc aaccttgcgt tcgctgaata
    19621 gtttttcgcg tttgcaggag atgagtggtc tgaaagagaa agcgggcgac cgttttgtgg
    19681 cgctggattc cccctttaac cactgcgaac agggcaaaat tgttattccc cggatgcgct
    19741 ttgagccttc catcgacaac gaagagcaac acattgccga aatggcggcc tttttccgtg
    19801 aacaggtgga gagcaaaaaa tatctcggta tgttggtact gtttgccagc ggacgggcga
    19861 tgcagcgctt tctcgactat gtgacggatt tacgcctgat gttgctggtg cagggcgatc
    19921 agccgcgtta tcgcttagtt gaactgcacc gcaaacgcgt tgccaacggt gagcgtagcg
    19981 tgctggtggg cttacagtca tttgccgaag ggcttgattt gaaaggtgat ctgctcagcc
    20041 aggtgcatat ccacaaaatc gcttttccgc ccatcgacag cccggtggtg atcaccgaag
    20101 gggaatggct gaaaagcctc aaccgctatc cgtttgaggt gcaaagcctg ccgagcgcct
    20161 cgtttaacct gattcagcag gttgggcgac tgattcgaag ccacggttgc tggggcgaag
    20221 tggttatcta cgacaaacgc ttgctgacaa aaaattatgg caagcgacta ctggatgcat
    20281 taccggtatt tccgatagag caaccggaag tccctgaagg tatagttaaa aagaaagaaa
    20341 aaacgaaatc cccacgccgt cggcggcgtt aatgatgtga gtcaggtaag gagtcgtaaa
    20401 tggactatcg caaaatcatt aaagagatcg ggcgcgggaa aaaccacgcg cgcgatttag
    20461 accgggatac tgcgcgcggt ctgtatgctc atatgctcaa tggtgaagtc cctgaccttg
    20521 agttgggcgg cgtattgatt gcgctgcgta tcaaagggga aggggaagca gagatgctcg
    20581 gcttttacga agccatgcaa aatcacacca tcaagctgac gccgcctgca ggcaagccga
    20641 tgccgattgt catccccagt tacaacggcg cgcgtaaaca ggctaacctg acgccattat
    20701 tggcgattct gctgcataaa ctcggttttc ctgtggtggt tcacggagtt agcgaagatc
    20761 caactcgcgt gctgacagaa accattttcg aactgatggg tattacacca acgttgcacg
    20821 gcgggcaggc gcaggcgaag ctcgacgaac atcaaccggt gtttatgcct gtcggcgcgt
    20881 tttgcccgcc gctggaaaaa caactggcga tgcgctggcg gatgggcgtg cgtaacagtg
    20941 cacataccct ggcgaaactg gcgacgccat ttgccgaagg tgaggcactg tgtctttcca
    21001 gcgtttcgca tccggaatac attggacgcg tcgcgaagtt ctttagcgat atcggcgggt
    21061 gggcgttact aatgcatggt actgaaggcg aagtgtatgc taatccgcag cgctgcccgc
    21121 agatcaatct cattgaccgt gaagggatgc gggtgctgta tgaaaaacag gaccctgctg
    21181 gtagcgagtt actgccacaa gcaaaagatc cggaaaccac ggcgcagtgg actgagcgtt
    21241 gcctggctgg cagcgaagcg attcccgaat cgctgaaaat ccagatggct tgctgcctgg
    21301 tggctacggg tgaagcggca actatcagcg atggcctggc gcgcgttaat caggcatttt
    21361 aatttttatc tcagccggat gagccacgtc atccggcttt taccccaccg caatatgaaa
    21421 ttcctgcatc tttattgacc ttcccgcgcc cggcgtgcag cataaaaaaa caacaaacac
    21481 ataacataaa caggagttaa ccatggaaag tggtcatcgc tttgatgctc agacgctgca
    21541 cagttttatt caggctgtat ttcgtcagat gggtagcgag gaacaagaag cgaaattagt
    21601 tgccgatcat ttaatcgcgg caaacctggc agggcatgat tctcatggta ttggcatgat
    21661 cccaagctat gtgcgttcct ggagtcaggg gcatctgcaa attaaccatc atgccaaaat
    21721 cgttaaagag gcgggggcgg cagtcacgct cgatggcgat cgcgcattcg gtcaggtcgc
    21781 ggcacacgaa gcgatggcgc tggggattga gaaagcgcat cagcacggta ttgctgccgt
    21841 ggcgctacat aactcgcatc atatcggccg tatcggttac tgggcggagc agtgttcagc
    21901 ggcggggttt gtctctatcc actttgttag cgtggtcggt attccaatgg tcgcgccgtt
    21961 ccacggtcgc gacagccgct ttggcaccaa tcccttctgt gtggttttcc ctcgtaaaga
    22021 taatttcccg ctgttgcttg attacgccac cagcgccatt gcatttggca aaacccgcgt
    22081 cgcctggcat aaaggcgtcc ccgtgccgcc aggttgcctg attgacgtta acggcgtgcc
    22141 gacgaccaat ccggcggtaa tgcaggagtc gccgttgggg tcgctgttga cctttgccga
    22201 acataaaggc tacgcccttg cagcgatgtg tgaaattctt ggcggggcgc tttctggcgg
    22261 taaaacgacg catcaggaaa cgttgcaaac cagccccgat gccattctta actgcatgac
    22321 cactatcatc atcaacccgg aactgttcgg cgcgccggat tgtaacgcgc agaccgaagc
    22381 ctttgccgag tgggtgaaag cctcgccgca tgatgacgat aagccgattt tgctaccggg
    22441 cgagtgggaa gtgaacacgc gtcgcgaacg gcaggagcag gggattccat tggatgcggg
    22501 aagctggcag gccatttgtg atgcggcgcg gcagattggt atgtcggaag agacgttgca
    22561 ggctttctgt cagcagttag ccagctaaaa aaaagcccgt ccagtggcgg acgggcaaac
    22621 aagggtaaca taggatcaat gagggttaga gcatatgcgt ctgtcggcaa acagacaggg
    22681 aaatacttgt gctggacgta gcgtaaacgc ctgatccgtc caacggttcg gattttgtag
    22741 gcctgataag acgcggtagc gtcgcatcag gcatctgttg ccggatgcgg cataaacgcc
    22801 ttatccgtcc tacgaattac ttataaatta ccgcagtacc gcttaattta ttgttgttgg
    22861 tggcggaag
//
LOCUS       DADLTY010000054        22866 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.054, whole genome shotgun sequence.
ACCESSION   DADLTY010000054 DADLTY010000000
VERSION     DADLTY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22866
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.054"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="JN334_001772"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="JN334_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HAY7437939.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1207..1869
                     /locus_tag="JN334_001773"
     CDS             1207..1869
                     /locus_tag="JN334_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7437940.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="JN334_001774"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="JN334_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HAY7437941.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /locus_tag="JN334_001775"
                     /pseudo
     CDS             2403..2757
                     /locus_tag="JN334_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2795..3100
                     /locus_tag="JN334_001776"
     CDS             2795..3100
                     /locus_tag="JN334_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY7437942.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="JN334_001777"
     CDS             3103..3507
                     /locus_tag="JN334_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437943.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="JN334_001778"
     CDS             3497..3796
                     /locus_tag="JN334_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437944.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3892..4278
                     /locus_tag="JN334_001779"
     CDS             3892..4278
                     /locus_tag="JN334_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY7437945.1"
                     /translation="MILSIDSNDANSAPLHKKTISSLSGAVESMMKKLEDVGVLVAHI
                     LMPILFITAGWGKITAYSGTQQYMEAMGVPGFMLLTAFLFHSNFAEGVNSLMFMKNLT
                     ISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4348..5334
                     /locus_tag="JN334_001780"
     CDS             4348..5334
                     /locus_tag="JN334_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY7437946.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="JN334_001781"
     CDS             5627..5995
                     /locus_tag="JN334_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY7437947.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6202..7098)
                     /locus_tag="JN334_001782"
     CDS             complement(6202..7098)
                     /locus_tag="JN334_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7437948.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7203..7904
                     /locus_tag="JN334_001783"
     CDS             7203..7904
                     /locus_tag="JN334_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY7437949.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7927..8091
                     /locus_tag="JN334_001784"
     CDS             7927..8091
                     /locus_tag="JN334_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437950.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8303..9613)
                     /locus_tag="JN334_001785"
     CDS             complement(8303..9613)
                     /locus_tag="JN334_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAY7437951.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCELSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9641..10972)
                     /locus_tag="JN334_001786"
     CDS             complement(9641..10972)
                     /locus_tag="JN334_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY7437952.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="JN334_001787"
     CDS             complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="JN334_001787"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY7437953.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12683..13072)
                     /locus_tag="JN334_001788"
     CDS             complement(12683..13072)
                     /locus_tag="JN334_001788"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HAY7437954.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="JN334_001789"
     CDS             complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="JN334_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HAY7437955.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="JN334_001790"
     CDS             complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="JN334_001790"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HAY7437956.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="JN334_001791"
     CDS             complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="JN334_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HAY7437957.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="JN334_001792"
     CDS             complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="JN334_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HAY7437958.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="JN334_001793"
     CDS             complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="JN334_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HAY7437959.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20217..20561
                     /gene="tdcR"
                     /locus_tag="JN334_001794"
     CDS             20217..20561
                     /gene="tdcR"
                     /locus_tag="JN334_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HAY7437960.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20817..21357
                     /locus_tag="JN334_001795"
                     /pseudo
     CDS             20817..21357
                     /locus_tag="JN334_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21379..21726
                     /locus_tag="JN334_001796"
     CDS             21379..21726
                     /locus_tag="JN334_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437961.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21925..22384
                     /locus_tag="JN334_001797"
                     /pseudo
     CDS             21925..22384
                     /locus_tag="JN334_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005089572.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         6169 a         5762 c         5271 g         5664 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatat ttccgccagt agtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtaga gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc atcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cggccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaattgt tgacaaagtg cgctttgttt atgccggatg
     6061 cggcgtaaac gccttatcca gcctacaaat tgccaaaatt caatatattg cagtgactgc
     6121 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc
     6181 ttgtgaaagg tgcttaatag attattttcc agcgaacaac tttggaattt cccgcagaca
     6241 ccaggatttc gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct
     6301 ggtcgattcc gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg
     6361 atacggcatc gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt
     6421 gctcaccgtt aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg
     6481 agcggtatcc gctaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc
     6541 cggctcctga tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag
     6601 cttgcgcgag ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacgatatc
     6661 cgcccgcccc tgctccagcc gttcccacgc ccccgccagc acttctgtga tgattgccag
     6721 ttgggtattg gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac
     6781 cagcgcttcg gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc
     6841 agtagtcagt ttatctgcgg cttccagcaa aacgcgccct cgctccagca acatcctccc
     6901 gacattggtg aatttggtgc gatggcccga gcggtcaaac agcaccacat ccagctcttc
     6961 ttccagtttt tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgctgc
     7021 cgccgcaaaa ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa
     7081 tgccctttct ttggccattt cgttctcatt caggaaattt gaacataccg ggcagaatat
     7141 ctggctaaca atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa
     7201 ttatgattac tacccgaact gccaggcagt gtggacaagc agactacgga tggttgcagg
     7261 cccggtatac tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc
     7321 tgcgtgtgct taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc
     7381 ccaaagtcga tattttaaat gtgattctgg atggagaagc agagtatcgc gacagcgaag
     7441 gcaatcatgt tcaggccagc gccggagagg cgttgctgct ctctacccag ccgggtgtca
     7501 gctatagcga acacaatctc agcaaagaca aaccgttaac gcgaatgcag ctttggctgg
     7561 atacctgccc gcagcgagag aatccgctga ttcaaaagct ggcgcttaat atggacaagc
     7621 agcaattaat cgcctcgcca gagggtgcga tgggaagcct gcaattacgc caacaagtgt
     7681 ggctgcacca tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac
     7741 gcgcgtattt gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag
     7801 cggcgctaac ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg
     7861 ccgattcccc actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag
     7921 atgatgatga gtaaaaaatc gaccaaaaag cgccagccag cgaagcccgt ggtggcgaaa
     7981 gaacctgctc gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagct
     8041 atcgtcgcgg atgctgaaac gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt
     8101 gccggatgcg gcgtaaacgc cttatccggc ctacaaaaca caacaacatc aacaaattaa
     8161 acaccccgta ggccggataa gatgcgccag catcgcatcc ggcatcaggt acaaaacaca
     8221 acaacatcaa caagttgagc accccgtagg ccggataagg tgcgccagca tcgcatccgg
     8281 cacaatactc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
     8341 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa
     8401 catcatgcgc cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
     8461 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac
     8521 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt
     8581 cataacgccc atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg
     8641 cggcacaaag tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag
     8701 aaagcatcag cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca
     8761 ccacaggcat tattgcagtg atcccctggt tacccgagcc ggagttactc atagccggaa
     8821 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
     8881 agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca
     8941 gcccccactt accgctcaaa ccttcctgcg ataactcaca atttagcttc gcggaatcga
     9001 gaataaagcg gatcgccgca aatgggactt cattgacgaa cttcaggatc tcagccagcg
     9061 tcgttctgga aagcaccgtc agcggcgact cttgctcgcc ctctgccaca cacgcctggt
     9121 gggtaaacac cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
     9181 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
     9241 tgcaaggttc ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg
     9301 taattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta
     9361 acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc
     9421 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
     9481 ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca
     9541 gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag
     9601 tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca
     9661 gagaaacaca aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt
     9721 tgtgcaatgc cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga
     9781 aaatcgggct acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc
     9841 aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct
     9901 cggcaggcat cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc
     9961 ctaaatagac gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca
    10021 ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa
    10081 tattctgctc gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg
    10141 cgtagaaaaa gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt
    10201 tatgccgcgc cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta
    10261 acgtctggat aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca
    10321 ccagcagccc cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg
    10381 acacgcccag cgcagcgact accagcagct tggtcagcac catgtcggtc gaaattttga
    10441 ataacaattt ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat
    10501 aaaagggact gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac
    10561 tatcgttggt gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga
    10621 gcgcacctaa caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat
    10681 ctttacattc tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg
    10741 ccgggtaacc aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca
    10801 ccgggagaaa aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg
    10861 tgtcagtact gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac
    10921 ccgccggggt cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatttctc
    10981 gctcaatatt ttgtagggct tattattttt ttccgagccg catcaaggcg atatgcggtc
    11041 tacgtttttg caggcgagtt attagaataa agaaatgata cgcgcgaggt cagagaaaat
    11101 cttcacaatc aagactttaa aataacggag atggataaga attttctact aaattaatca
    11161 caagagaata ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca
    11221 cccaaggatg aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg
    11281 cgtgatgttt cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc
    11341 actttatcga gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt
    11401 actgctttca cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca
    11461 accggatcgc aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc
    11521 tgcgccggac tgccgcccag tagttcagtt aaccctgccg ctgccattga acaggccacg
    11581 ccaatctccc cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc
    11641 gcgccaatag cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga
    11701 cggaacttat cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt
    11761 gccgttacca cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg
    11821 atccagtcga tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga
    11881 cgcagcgcta cggcacgaca cggcacattg agcggaccag gcagcacgcc ttcagtgttc
    11941 atcccgcgtt caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt
    12001 tccgctttgc tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg
    12061 ttgtaatcac acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt
    12121 tcgacatcgt gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag
    12181 taataggttt tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc
    12241 tcatggcgag gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca
    12301 atatgcgcac cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca
    12361 gggatctcat caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg
    12421 acatccgtgg catggccttt gcccgtcagt gacaacgacc cgtacagatc gaccacaata
    12481 tggctcgtcg cggttaataa gccgctactt tccagccgat caataaaact ttttccggca
    12541 ttcattggcc ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat
    12601 gcactaatca tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg
    12661 cgaccgtccc gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct
    12721 tcacatcttt cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat
    12781 gctcatcgaa gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga
    12841 tgaacacggt catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt
    12901 tgacgttttc gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct
    12961 gcgggcaaac cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa
    13021 cataagggcc gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc
    13081 cggcgtcaga gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc
    13141 agtgcgttga agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc
    13201 gggtgctcga tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga
    13261 ccgccttcga catccgcttc gtggtggaaa tacccatcca gcaggccgac aaggttggtt
    13321 ttacgtactg gatcttcttt gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc
    13381 ccatctttgg cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct
    13441 ttgcggtcac gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga
    13501 ccgtccggcg tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc
    13561 gactgggtag ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt
    13621 tcaaccaggt cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat
    13681 tcaccgtcga tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg
    13741 gcatatttga tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc
    13801 atagtgcgat agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg
    13861 tcgtgcatgt agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg
    13921 aagtgatcga ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct
    13981 gttttcggcc cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc
    14041 agcgttttcg ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt
    14101 gggctgacgc agcaggcaat cgcgtaatcg tcgctgttga agtcagtacg catcagatcg
    14161 tcattttcat actgcaagga agaggtgacg atcgacacct gcgcggcata ttttttgaag
    14221 gcaatcggta attcttccga ccaaagaatg gtcaggttag gttccggtgc cggccccata
    14281 gtgtgcaggg tgtgcaaata gcggaaggag tttttggtca ccagcgtacg accgtccagc
    14341 cccatcccgc cgatcacttc cgtcgcccag attgggtcgc cggagaacag cgaatcaaat
    14401 tccggtgtac gcaggaagcg taccatacgg atcttcatga tgaagtgatc gatcagttcc
    14461 tgtgcctgct gctcattgag gacgccagct ttaaagtcgc gctcaatgta gatatccagg
    14521 aacgatgccg tgcgccccag cgacatcgcg ccgccatttt gcgatttcac tgccgccaga
    14581 taagcgaagt agagccactg caccgcttcc tgtgcattct gcgccgggcg agagatatca
    14641 aagccatatt tcgccgccat ttcctgaatc tgcaacagcg catgacgatg ctctgccagc
    14701 tcctcacgca gacggatggt ggcttccaga tcctcgcctt tttccagacg agactggaga
    14761 tcggcaaatt gcagttcgcg ttcacgcacc agataactga tgccatacag cgctacgcgg
    14821 cgatagtcac cgataatgcg cccacgacca tagccatctg gtaaaccggt cagcacgcca
    14881 gatttacggc agcgcagcat atccggtgag taaacatcaa atacgccctg gttatgggtt
    14941 ttacgcagat cggtaaacag atattcaaat tcactgtcca tttcacggcc ataggcgtgg
    15001 aatgaacttt taatcatatt aatgccaccg aacgggtgta gcgcacgttt caacggcgca
    15061 tccgtttgca ggccaacaat tttttccagc ggctggttaa tatatcccgc atcatgagcg
    15121 gtaattgtgg tggcaatatt ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata
    15181 cggatgcctt ccattacctt ttcccacaat tccgtggtgg caggcgtcgc ttcggcgaga
    15241 aaagattcat cgccttcata cggtgtatag ttatgttgaa taaaatcgcg gacattaatt
    15301 tcgcttttcc agtccgtacc tttaaagcca agccatgcgt cagcgtacag cttatcgctg
    15361 gtatcaatat ctaccttcat gaaaaataat ctctctacaa tacttcaact aaattatgca
    15421 aattctgcgg gcgcgttaac tttacctaaa tgaatggcat ccaaagcaat cattttttct
    15481 tcgttagtcg gaataacgac gcaaatgaca tgcgcatttt cactggaaac aattcgctcg
    15541 ccaaaagagt tagaacgatt attcatttct gtatcaatct ctacgcctaa tacagccaaa
    15601 tgttccatga ccagacgacg aattaaactt gaattctctc ctattccgcc ggtgaatata
    15661 attccatcca gccgacgtaa tgaagctgca tgtccggcaa tatgacgggc aattcggtga
    15721 acaaaggttt taattgccag ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga
    15781 acacgtaaat ccgaagaaag accggaaata cctaataatc ccgactcttt attcactacg
    15841 cgttccaggt cacccaggct ctggttggtt tggctggcga cccaggacat cgcaccaaag
    15901 tcgacatcgc cactgcgggt acccatcatc aagccttcca gcggcgtcat tcccattgag
    15961 gtgtcaacac tctgaccgtt gcgaactgcg cagattgacg cgccattgcc aagatgcgcc
    16021 acaaccaggc cggagtcatc ttccgccaga ttcagcagcg aatgtgcgcg ctgggaaaca
    16081 tagcggtgcg acgtgccgtg gaaaccataa cggcgtacac ctaactcttc ataatatttc
    16141 cacggcaggc cgtataaata agcttccgga gccatcgtct ggtggaaact ggtatcaaat
    16201 accgccacct gagttacgcc cggaaataat tgctgcgccg attcaatacc acttaaattt
    16261 gcgtaattat gcaggggtgc cagtggagaa acgcgacgga tattatcaat gacttcatcg
    16321 gtaataatgg cggactcggt aaaaatactg ccgccgtgag cgatgcggtg gccaattaag
    16381 gccacgctgt catttaaatt ccgtttttcc agttcaaatg caattgcctt caatgcacct
    16441 tcgtagctgt ggtgagccag cggtgctggc tctcccccat ttacggataa gaatgcattt
    16501 tccgagttaa taccgtcggc aatacctgac attaatactt cacaatcgct ggcatcgagt
    16561 acggaaaact taatcgaaga cgaaccacag ttaataacca aaacaaccgg aaattcattc
    16621 atctcttttc tcatcctgag ttacggatta aaacagtttg tatacgatgt tcaggatggt
    16681 cagcagacca atcacggtaa caaacacgtt atccagacga ccacggtatt tcgccagaga
    16741 cggcgcttta cggatagcat acatcggcaa caggcacagc agggatgcga taatcggtgc
    16801 gcccatggct tcaatcaggt cgaggatgtt cgggttggcg taggcaacaa cccaggtgga
    16861 gcccatgatg aagatcatgc tgagagtatt cagtttaccc agcgacactt tggttttgtc
    16921 acctttataa ccgaacttca gaatcagacc attcaagcct tccagcgtcc ccagatagtg
    16981 accgaagaaa gatttgaaga tagccacgag tgcgatgatg gaagccgcat attccagtgt
    17041 aatcgcgaac gttgttttcg taccggtcat ggacgcaaag tggttagcca gataagaaag
    17101 cactggaata ttctgcgctt tggcttccgc catgttggcc ggagacagag taaacaggca
    17161 gctaaaggca aagaacatca ccactgcaac catcagcatg ctggcacgag aaatgatttg
    17221 ggaacattta cgttcggtga agtcgcgacc gaagtctttc tcatactctt cacgtttaga
    17281 aaccacgaag gaagagacga ttggcgagaa gttaaaggag aaaaccatga tggaaatccc
    17341 cagccagaca gtgatcagga taccgtcatg accggttaac gacagcgaac cgaggtcaac
    17401 ctggtcgata actgcagagt tccagtaagg gatcagcgac agagaaatca gcaccaggct
    17461 ggcgataaac ggccatacca ggtagctcat cactttaacc atcagatcct taccaaacca
    17521 gatgacgaaa gccatcagca gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc
    17581 aaatccgagc tggttttccc agaacgtcat aaaggtattg gtaatagtaa cgccataaat
    17641 ccacagcagt gggcaaatcg cgaagaagta caggaacgtg ataaccacgc cgccagtttt
    17701 accaaaatgc tcttccaccg tttccgtaat gttgccggaa gggttagagc cagaaagaca
    17761 cagacgcgcc agcgcccggt ggcaataaaa tgcgatgggg tatgccaata ccaacatcag
    17821 aagaatcggg atcagtccgc caaaacctgc gcggataggg aagaacagca ccccggcgcc
    17881 gattgccgta ccaaacaagc ctaacgtcca tgtggtatct gatttacgcc aggacgattg
    17941 ttttgtctgg ctggatacaa tgctatctga agtactcata tcctatcctc aacgaattaa
    18001 ttaagcgtca acgaaaccgg tgatttgaga gacgcgagaa agatcgatat tgccgccgga
    18061 aataatactg acggtttttc tattttgaat atattggtct aatttaccgc ttaataatgc
    18121 agcacatgcc agagcgcctg cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat
    18181 catactgttt ctgatttcgt cttcgctgac cagcacgatg tcatcgacta attcacgaac
    18241 gatttcgtaa gttaaattac ccgggcggga gacatcacaa ccatccgcca gggtgccggt
    18301 agttcggtgc gtggttattt ctccggagtg gaaagaagcc gccatgccgt gaacgttttc
    18361 tgactgtacg ccaataacac gaatggtcgg gttaatagat ttaattgcca ccgcaatacc
    18421 agcaattaaa ccaccaccac caattggcac aatcacgtta tcgacatcat agagatcttc
    18481 cataatttcc agaccaatcg ttccctggcc agcaatcact ttcggatcat cgtaaggtgg
    18541 gataaaaata cgaccttcca tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa
    18601 gttatcacca tgcagaacga cttctgcgga gtagtcgcac gttgccgcta ctttggattt
    18661 tggcgcacct tttggcatca ccactttacc gtcgataccc agcatcgcgc aggagagcga
    18721 aaccccttgc gcatggttgc ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc
    18781 ggtcagtgaa cttaatttat taaatgcgcc acgaatttta aatgaacccg tacgctgcat
    18841 attttcaaac ttcaggaata tttcaccttt gcaacgttca ctaaaatagt tggagcgagg
    18901 catgcctgtt ttataaattc gcccagccag tcgttgtttc gcttcaataa tatcatcaat
    18961 agcaaccggc agatcgtatg taatatgcat tataaaacct cttcgcctga attaaatatg
    19021 taggtaaccg taaccgacac ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa
    19081 acaaacaact aaccaacttc tattaattgc cttcgtctac acccattata agatgaatac
    19141 tctttggcta attccaccaa aaccgatgct gcttttttaa tacgataatt tttcgaccat
    19201 accgcggcat attgtgccac aggtaatgtt tcttcaaccg gaatagtaat aaattgatta
    19261 gaaccaaaag gtgacgtcat atcacaagga attacagtta agaaatcagc attgagaaca
    19321 agattataaa ttgtcacgac tgagtcggtt ttaacgatgt tttcaatact gatgccattt
    19381 ctttgtaacg tagtaagcag ttcgctgtag taccccatat tagtttgtgg caacacccac
    19441 tgttcgttct tcaacgactc cagcgtggtg gtgccggtgc atgttcggga cttactggct
    19501 accagcacaa actcggactc gaacagcggc tcaacatgta aatcctgaag cttcatttct
    19561 gcacttaacg taccaatcgc aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa
    19621 gacagttgcg cttcatacat agaaacctgc gctttcggga acacctcttt gaacttgttg
    19681 atcatccctg acataaaagt aaaaccaatc aatgaaggaa aaccaaatga gacttccacc
    19741 accgcttcag aagacatacc gcttatctca ttaaccatat ttttcatttc acgggtaatg
    19801 gattcggaac gggagagtaa caattgaccg gcaggtgtta atgttacacc ggtatttttc
    19861 cgcaccacta attccacacc aaaataatct tcaatatcgt taatgatttt actgacggcc
    19921 ggttgagtta accctaattc ttttgcagcc gagccgatag agccacttct aatgacttcc
    19981 tgaaagacta ccaggtgctg cgttttcgga agaagaatag tgctcataac gacctacgtt
    20041 aattacctca ttgacggcat gaagtgtatc aaaatgaaat aaacaggata tgtgcgacca
    20101 ctcacaaatt aactttcatt actttccaag gtatcgttat tacaattaaa catctttaaa
    20161 tatcaacaag ttaaagcata aaaatcagca taaaccctga tttttatcaa aaaaaaatga
    20221 cggggataac catattttat ggcgataaca tcattcgtta tgtggttaat acaaaaaagg
    20281 ggctgagacc atatttcaag caattaccgg ataattacca ggcaaaattt gagttaaatt
    20341 taatgagcaa gttctcaaat tttattataa ataaaccatt ttcagcgata aatactgcgg
    20401 cacgtcacat tttttcacgc tatttgttgg agaacaaaca tttattttat caatatttta
    20461 aaatttcgaa tacaggtatt gatcatctcg aacaattgat taacgtcaac tttttctctt
    20521 ctgacaggac gtcattttgt gaatgcaatc gttttccata aattcttctc ccctcatagg
    20581 cgacgaatag cattttgtgt tgaggatcac aaaacgaata attgctggtc gccgcgataa
    20641 ggtcagacga agtcaacaag ggggattttc actgtgcttt acatcagcgt aggacacaga
    20701 atgttgcttc gtttactgta acgccgggta aatgagcgtt ttttgatagt gcgtaagtgt
    20761 cgtgcgagga aaataagcat ctattattgt ttgtcatgac gacaaggcag gcatcaatga
    20821 aaggcttccc aattgcacat atttttcacc cttcaatccc gccaatgcac gcagtggtta
    20881 acaatcacaa tagaaatatt gattattgga cggtaaaaag aaagtttgca gaaattgtct
    20941 ccaccaatga cgttaataaa atttacagta taagtaatga actccggaga gtattatctg
    21001 caattactgc attgaatttc tatcaaggcg atgttccttc tgtcatgatt ccgaatccaa
    21061 ccggaaaata tgagtccatt catcatagat atttctacag gagaacatga tgattatatc
    21121 atacaaacat tagatgtagg cacttttgca ccttttggtg aacaatgtac ttgctcagcc
    21181 gtcaataaaa aagagctgga atgtattaag gagacgattt ctaagtattg cgcaaagttc
    21241 acccaaaaag aagccatctt aacaccccct aggcacttta ataaaacctg cattacttca
    21301 gattgttggc aaattctctt tttttcaccc gatcatttta ataatgattt ttattgatta
    21361 tttataaagg ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat
    21421 ttagtccgtc gaaaaatgaa tgaccttcct gaaagcccca taggaaataa cctcgaagca
    21481 ctagcaccag gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac
    21541 gcgttacaac caagtggaaa atgcattatc actggtgatt ttcaaaaaga attagcgtac
    21601 ttacaaaatg taattcttta taatgactcg tctcttcgtc tggatttttt aggttataac
    21661 acccaaatta ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa
    21721 aactaggaaa tatctacagg caatatcaat gttaattgcc cattaaaaga tatttacaat
    21781 gaaatcagga ggttgaacgt aatctttagt tgtgggactg gagatatagt tgatctatcc
    21841 tctctggact tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct
    21901 aatactattc taaatcattt taaattggat tcaacaaatt ttactaatgc caacatgttt
    21961 caggttaatt ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa
    22021 ataacgcctg ttttaataag cattagcgat atgtattctg aagaaaaaat caagtttgtc
    22081 gaaagttgtt taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt
    22141 gcaattataa aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca
    22201 aaagaaatat ataaaagctc atcgaaaatc aacaattgct tgaacagaat taaattacca
    22261 gagatgaaag aattctcatc aaaaaaaaca tatgattaca tcgatacaat cattgaagat
    22321 tatgaaaata tcaaagaaaa tgcttatctg gtcgtccccc aaattaatta tactatggat
    22381 ttaaacatag aagactctag ctcagaagag ttaataccct cgagaaagac gaaaattctc
    22441 cggacaatgg ctttgaggtc ggggaatata acacatatga agcatataac tcagagaatc
    22501 aatattttac cagagaggac tatacgtata attacgacct tttaaatgca atatagactt
    22561 aataacttct tacaaagtct attattaata ccgtagatat tcatcttacc ctgtgcacca
    22621 aaagcaaaaa cttatcgacg cgggtttgaa actgatggca accgcaaaaa tgcctgatgc
    22681 gctacgctta tcaggcctac gccatctctg caatatattg aatttgcgtg cttttgtagg
    22741 caggataagg cgttcacgca gcatccggca tgaataaagc gcactcaaca atctgaaacc
    22801 cgccggaacg gtttattacg tacatcaggt gaaactgacc gataagccgc tttcttttgg
    22861 gtatag
//
LOCUS       DADLTY010000055        22574 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.055, whole genome shotgun sequence.
ACCESSION   DADLTY010000055 DADLTY010000000
VERSION     DADLTY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.055"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            187..480
                     /locus_tag="JN334_001798"
     CDS             187..480
                     /locus_tag="JN334_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY7437962.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="JN334_001799"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="JN334_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HAY7437963.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="JN334_001800"
     CDS             complement(1922..2374)
                     /locus_tag="JN334_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HAY7437964.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /locus_tag="JN334_001801"
     CDS             complement(2427..3761)
                     /locus_tag="JN334_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7437965.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="JN334_001802"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="JN334_001802"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAY7437966.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="JN334_001803"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="JN334_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HAY7437967.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="JN334_001804"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="JN334_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HAY7437968.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="JN334_001805"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="JN334_001805"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HAY7437969.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="JN334_001806"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="JN334_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HAY7437970.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="JN334_001807"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="JN334_001807"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAY7437971.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="JN334_001808"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="JN334_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAY7437972.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="JN334_001809"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="JN334_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HAY7437973.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="JN334_001810"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="JN334_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HAY7437974.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="JN334_001811"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="JN334_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HAY7437975.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="JN334_001812"
     CDS             complement(15085..15582)
                     /locus_tag="JN334_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAY7437976.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="JN334_001813"
     CDS             complement(15579..15941)
                     /locus_tag="JN334_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAY7437977.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="JN334_001814"
     CDS             complement(15931..16278)
                     /locus_tag="JN334_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HAY7437978.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16387..16800
                     /locus_tag="JN334_001815"
     CDS             16387..16800
                     /locus_tag="JN334_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7437979.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18313)
                     /locus_tag="JN334_001816"
                     /pseudo
     CDS             complement(16847..18313)
                     /locus_tag="JN334_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001665828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="JN334_001817"
     CDS             complement(18324..20039)
                     /locus_tag="JN334_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAY7437980.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="JN334_001818"
     CDS             20206..21099
                     /locus_tag="JN334_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7437981.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="JN334_001819"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="JN334_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inactive 6-phospho-alpha-glucosidase"
     gene            complement(21910..22572)
                     /locus_tag="JN334_001820"
     CDS             complement(21910..22572)
                     /locus_tag="JN334_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY7437982.1"
                     /translation="MLVGITEPLEFTFLFISPLLFAVHAVLAASMSTVMYLFGVVGNM
                     GGGLIDQVLPQNWIPMFSNHADMMLTQIAIGLCFTLLYFVVFRTLILQFNMCTPGRED
                     AEVKLYSKAEYKASRGQTTAAEPKKELDQAAGILQALGGVGNISSINNCATRLRIALH
                     DMSQTLDDEVFKKLGAHGVFRSGDAIQVIIGLHVSQLREQLDSLINSHQSAENVAITE
                     AV"
BASE COUNT         5739 a         6116 c         5665 g         5054 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaactt ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagcaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 acggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 ttcaaatatt actgtttatt tatacagtaa gcttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccgcc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttcg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcagaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat ttccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atgg
//
LOCUS       DADLTY010000056        22467 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.056, whole genome shotgun sequence.
ACCESSION   DADLTY010000056 DADLTY010000000
VERSION     DADLTY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.056"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            563..1333
                     /locus_tag="JN334_001821"
     CDS             563..1333
                     /locus_tag="JN334_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HAY7437983.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1324..1797
                     /locus_tag="JN334_001822"
     CDS             1324..1797
                     /locus_tag="JN334_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAY7437984.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1904..2443
                     /gene="dnaT"
                     /locus_tag="JN334_001823"
     CDS             1904..2443
                     /gene="dnaT"
                     /locus_tag="JN334_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HAY7437985.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2446..3183
                     /gene="dnaC"
                     /locus_tag="JN334_001824"
     CDS             2446..3183
                     /gene="dnaC"
                     /locus_tag="JN334_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAY7437986.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3232..3726
                     /locus_tag="JN334_001825"
     CDS             3232..3726
                     /locus_tag="JN334_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HAY7437987.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3966..6257
                     /gene="opgB"
                     /locus_tag="JN334_001826"
     CDS             3966..6257
                     /gene="opgB"
                     /locus_tag="JN334_001826"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HAY7437988.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6395..7417)
                     /locus_tag="JN334_001827"
                     /pseudo
     CDS             complement(6395..7417)
                     /locus_tag="JN334_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
     gene            7556..8470
                     /locus_tag="JN334_001828"
     CDS             7556..8470
                     /locus_tag="JN334_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7437989.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8685..10046
                     /locus_tag="JN334_001829"
     CDS             8685..10046
                     /locus_tag="JN334_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7437990.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="JN334_001830"
     CDS             complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="JN334_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY7437991.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="JN334_001831"
     CDS             complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="JN334_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HAY7437992.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12596..13885
                     /locus_tag="JN334_001832"
     CDS             12596..13885
                     /locus_tag="JN334_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HAY7437993.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13882..15348
                     /gene="hpaE"
                     /locus_tag="JN334_001833"
     CDS             13882..15348
                     /gene="hpaE"
                     /locus_tag="JN334_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HAY7437994.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15350..16201
                     /gene="hpaD"
                     /locus_tag="JN334_001834"
     CDS             15350..16201
                     /gene="hpaD"
                     /locus_tag="JN334_001834"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HAY7437995.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16211..16591
                     /locus_tag="JN334_001835"
     CDS             16211..16591
                     /locus_tag="JN334_001835"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HAY7437996.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16659..17462
                     /gene="hpaH"
                     /locus_tag="JN334_001836"
     CDS             16659..17462
                     /gene="hpaH"
                     /locus_tag="JN334_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HAY7437997.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17473..18276
                     /gene="hpaI"
                     /locus_tag="JN334_001837"
     CDS             17473..18276
                     /gene="hpaI"
                     /locus_tag="JN334_001837"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HAY7437998.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFGWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18298..19653
                     /gene="hpaX"
                     /locus_tag="JN334_001838"
     CDS             18298..19653
                     /gene="hpaX"
                     /locus_tag="JN334_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HAY7437999.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19663..20553
                     /gene="hpaA"
                     /locus_tag="JN334_001839"
     CDS             19663..20553
                     /gene="hpaA"
                     /locus_tag="JN334_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HAY7438000.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20661..21517
                     /locus_tag="JN334_001840"
                     /pseudo
     CDS             20661..21517
                     /locus_tag="JN334_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21744..22467
                     /locus_tag="JN334_001841"
                     /pseudo
     CDS             21744..22467
                     /locus_tag="JN334_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Pyoverdin chromophore biosynthetic protein pvcC"
BASE COUNT         5568 a         5884 c         5585 g         5430 t
ORIGIN      
        1 ttaaatgttt ccagcaaatt aatagattaa gcgaataaat tatccttagc attcactttc
       61 atatcagcat actcctgctg cacaacaact ccactgcatg tgtcagaagg ttagcatcaa
      121 atgataacct ggtgaaatag cctggagaca tttaaaatat ctacattaag ttgttagaaa
      181 tacttaatga aaaacagaaa tctcattcat ttaatttata attaaatgat atctcttccc
      241 gcagaatgcg ggaatcacac aatcaatatt tattcaataa aatctgatga ttttaacttg
      301 ttaacactaa aaaacgcgcc cagctatcta ttatcctgac ccgtaaaggc taaacaataa
      361 attcattata gtgataatat tcatgtgata gtttgtttgc ggcgagagat aattcgcttt
      421 ttatcaccga gcttgtccca ataaccagga ttaatcagcg caattttgat cttaacctac
      481 cgcaaaacaa ccgtgcttca tgatacaaat ggattctcat tatctgaaca atacgcagca
      541 cgtctatgac aaagggcgag ttatgcaaac tgagcaacag cgagccgtaa cgcggctttg
      601 tatccagtgt ggattatttc ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct
      661 ttcctcacga ctgggtcggg cgctgggaat ggacagcgtc gaaagttcta tctcttcgaa
      721 cgccatagtg ctgacaacta ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca
      781 cgatcgcggc attaatatgc atgtggtgac tgaagtccag cacattgtga ttcttgcgga
      841 gcatcatctg ctggattaca aaggcgtaga gaaacgattt agccaaattc aaccattacg
      901 ttacccaaga tggctggtag ccttaatggt tggcctttct tgcgcctgtt tctgtaaact
      961 caataacggt ggctgggatg gtgccgtcat caccttcttt gccagtacga ccgcgatgta
     1021 tatccgccag ctgctggcac aacgtcatct tcatccacag atcaactttt gccttaccgc
     1081 tttcgccgcc accaccattt ccggattgct tttgcaactc cccactttca gcaatacccc
     1141 caccattgcg atggccgcca gcgttctgct gctagtgccg ggctttccgt tgattaatgc
     1201 cgtcgccgat atgtttaaag gccacatcaa taccggactg gcacgctggg cgatcgccag
     1261 tctgctgaca ctggctacct gcgtcggcgt agtgatggca ctgacgattt gggggctacg
     1321 cggatgggtg tgatcgaatt tctgttagcg ttggcgcagg atatgatcct cgccgccatt
     1381 cctgcggtcg gctttgcgat ggtgttcaac gttcccgtgc gggcgttacg ctggtgtgcg
     1441 ctgcttggct cgataggtca tggttcccga atgatcttga tgaccagcgg gttgaatatt
     1501 gagtggtcaa cctttatggc ttctatgctg gtcggtacca ttggtattca atggtcgcgc
     1561 tggtatctgg cgcatccgaa agtgtttacc gtggcggccg ttatccctat gttcccgggc
     1621 atatcggctt ataccgcaat gatttcgtcg gtaaaaatca gccagttagg ttacagcgaa
     1681 ccgttgatga ttaccctgtt aaccaacttt cttacagctt catcgattgt tggtgcgtta
     1741 tccatcggtc tttccattcc tggattatgg ttgtaccgca agcgccctcg cgtataaaat
     1801 tgcctcgctt aagcacacgg atgagagaca gcctcctctc ctccgtgtgt tactataaaa
     1861 gttatctccc ttctcgttca tcgttccata tttgagaaac agtatgtctt ccagagtttt
     1921 gaccccggac gtcgttggta ttgacgccct ggtacacgat caccaaaccg ttctggcaaa
     1981 agctgaaggc ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac
     2041 gcctgcacgc ctggctgaac tgctggcgct ggaagaaaag ctagcgcgtc cgggaagcga
     2101 tgtcgctctg gacgatcaac tctatcagga accgcaagcc gctcccgttg ctgtacccat
     2161 ggggaaattc gccatgtatc cggactggca acccgatgcc gattttatcc gcctggcggc
     2221 gctatggggc gtggcgctaa gagagccggt gaccaccgaa gaactggcct cattcattgc
     2281 ctactggcag gcggaaggta aagtctttca ccatgtgcag tggcaacaaa aactggcgcg
     2341 cagcctgcaa atcggtcgtg ccagcaacgg cggactgccg aaacgagatg tgaatacggt
     2401 cagtgaacct gacagccaaa ttccaccagg attcagaggg taacgatgaa aaacgttggc
     2461 gacctgatgc aacgcctgca aaaaatgatg cctgcccata tcaaacctgc attcaaaacg
     2521 ggtgaagagc tactggcctg gcagaaagaa caaggggcga tccgctccgc cgctctcgaa
     2581 cgtgaaaatc gggcgatgaa aatgcagcgc acctttaacc gctccggtat tcgtccactg
     2641 catcagaact gctcctttga gaactatcgc gttgagtgtg aagggcagat gaatgcgtta
     2701 agcaaagcgc gccagtatgt cgaagagttt gacggcaaca tcgccagctt catcttttct
     2761 ggtaagccag gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga gctgctgcta
     2821 cgcggtaaat ccgtattgat catcaccgtg gccgatatta tgtcggcgat gaaagatacc
     2881 ttcaggaata gcggtaccag cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg
     2941 ctggtgatcg atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac
     3001 cagatcgtcg atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac caacagtaat
     3061 atggaagaga tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac
     3121 agtttatggg tgatcttcaa ctgggatagc taccgtagcc gggtaacggg taaagagtat
     3181 taatccccgt ttctggcggt atactgcgac acaattccac cttgagtaat gatgatgaaa
     3241 actattaaac atcttctgtg ctgtgccatt gccgccagcg cattaatttc caccggggtg
     3301 catgctgcgt cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac
     3361 agcacgacac aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct tagcagcgga
     3421 aaccaagcct taagcgcaga taacatgaac aatgccgcag gcattctgca atactgcgcg
     3481 aagcaaaagc tggcttcggt aaccgatgcc gaaaacatca agaaccaggt gctggaaaag
     3541 ctgggcctga acagtgaaga gcaaaaagaa gacaccaact atctggacgg tattcagggt
     3601 ttgctgaaaa caaaagatgg tcagcaactc aatctggata acatcggaac gactccgctg
     3661 gcagaaaagg tgaaaaccaa agcctgcgat ctggtgttaa agcaggggct gaacttcatt
     3721 tcctgaaccg cgaaatgtta cccggctgac gcggctctct cctgcccttt tccctgctcc
     3781 tggacggttt tccccctgtt acacgggaag cgaccgcgat caaaaaagtc tgtttataaa
     3841 ctaattccta acaacatcgc atttttttga cactagaatt gcagcaatat ggcgacgcca
     3901 ttacattgtg cttctcaaaa agcttaaacg ttggctaaac ggcccggttt agtgaggatc
     3961 gcccattgtc agaactactc tctttcgccc tttttctcgc ctctgtgctg atttacgcat
     4021 ggaaagcggg acgtaacacc tggtggtttg cagccacgtt aacggtgctg gggctatttg
     4081 tcgttttaaa tatcaccctg tttgccagcg actattttac tggcgatggt attaacgacg
     4141 cggttctcta taccttaacc aacagcctga ccggtgctgg cgtcagcaaa tacatcctgc
     4201 cgggtatcgg cattgtgctg gggctgacgg cggtgttcgg tgcgctgggc tggatcctgc
     4261 gccgtcgtcg tcatcatccg caccattttg gttacagcct gctggcgctc ttactggcgc
     4321 tgggttcggt ggacgccagc ccggcatttc gtcagataac ggaactggtg aaatcccagt
     4381 cacgcgatgg cgacccggac tttgcggctt attataaaga gccgtcgaaa actatccctg
     4441 acccgaaact caacctggtt tatatctacg gcgaaagtct cgagcggacc tattttgata
     4501 acgaagcttt cccggatctc acgcctgaac tgggcgcgtt gaaaaatgaa ggcctggatt
     4561 tcagccacac gcagcagctg ccaggaacgg attacacgat tgcgggcatg gtggcttctc
     4621 agtgcggcat accgctgttc gccccctttg aaggcaacgc ctccgcctct gtctccagct
     4681 tcttcccgca gaacatctgt ctgggcgata tcctgaaaaa ctcgggttat cagaactatt
     4741 tcgtgcaggg cgcaaatctg cgttttgccg gtaaagatgt gttcctgaaa tcgcacggct
     4801 tcgaccactt atacggctca gaagagctga aaagcgtggt agccgacccg cactatcgca
     4861 acgactgggg attctacgac gataccgttc tcgatgaagc gtggaaaaag tttgaagagc
     4921 tttcccgctc aggtcagcga ttctcactgt ttaccctgac agtcgatacc catcacccgg
     4981 atggttttat ctcgcgcacc tgtaaccgca aaaaatatga ttttgacggt aagccgaatc
     5041 agtcattcag cgcggtaagt tgcagccagg agaatatcgc gacgtttatc aacaaaatca
     5101 aagcgtcacc gtggtttaaa gatactgtca tcgtcgtctc ttctgaccat ttagcgatga
     5161 acaacacggc gtggaaatac ctcaataagc aggatcgcaa taacctgttt tttgtcattc
     5221 gtggcgacaa gccgcagcaa aagacgctgg cagtgaagcg taacacgatg gataacggcg
     5281 cgacggtgct ggacattctc ggtggcgata actatctcgg acttggtcgt agcagtttat
     5341 ccgggcagtc gatgtcggaa atcttcctca atatcaaaga gaaaacattg gcgtggaagc
     5401 cagatatcat ccgcctgtgg aaattcccta aagagatgaa agagttcacc atcgaccagc
     5461 agaaaaacat gattgccttc tcgggtagcc atttccgttt gccgctgctg ttgcgggttt
     5521 cagacaaacg cgtggaaccg ctgccggaaa gcgaatactc agcaccgctg cgtttccagc
     5581 tggccgattt cgctccacgc gacaatttcg tctgggttga cagttgctac aagatggcac
     5641 aactctgggc tccggaactg gcactttcca ccgactggtg tgtctcgcag gggcagcttg
     5701 gcggtcagca aattgttcag catgttgaca aaacaacatg gaagagcaag acggcattta
     5761 aagatacggt catcgacatg gcgcgttaca aaggcaatgt cgatacgctg aagattgttg
     5821 ataacgatat tcgctacaaa gccgacagtt tcatctttaa cgtcgccggt gcgccagaag
     5881 aggtgaaaca gtttagcggg atttcccgtc cggagtcgtg gggccgctgg tccaacgcgc
     5941 agctgggcga tgaagtaaaa atcgagtaca agcatccgct gccgaagaaa tttgacctgg
     6001 tgattaccgc caaagcatac ggcaataacg ccagccgtcc tattccggta cgcgtaggca
     6061 atgaagaaca aactcttgtg ctgggcaatg aagtgaccac caccacactg catttcgata
     6121 acccaaccga tgccgacaca ctggtaattg tgccgccgga acctgtctca accaacgaag
     6181 ggaatatcct cggacactcg ccgcgtaagc tcgggatcgg catggtggaa attaaagtgg
     6241 tagaacgtga agggtaattc ctgactgatg tgtaggtcgc atagggtgcg acctacacgc
     6301 cttctccggc ttacaacaca ttattaagaa acaaaaaaaa cagcgcccaa cacttccccg
     6361 gacgctgcat ggcaacttta ataatctcgc cttatcagaa agtaattaca cccttaatca
     6421 actcacgatt gttaatcaca tcgcgctcgt aggtttctgc cagcgtggcg aacggatagc
     6481 gatgggttaa catcatgtcg gcagtgattt tcccttccgc catcagtcga ccgactttag
     6541 cgaaatcttc cggcgtggcg ttgcggctgc ccatcatcgt cgtttctttt ttatggaatt
     6601 ccggatcgga gaactgcaac tcacctttaa acagaccgac aaataccacc gtgccgccgt
     6661 gacgaatcag attcacggta ttattcatcg catgttgatt acctgtcgcg tcgatcactt
     6721 tctgcgccag cgaaccacca aactgcgccc gcagctgcgc gtcaaaatct tcggctgacg
     6781 ggtccagcac gggcaactcc agacgcgttg ccacatgttc acggcgcgcc ggactggtat
     6841 ccgccaccac cacctgtgcg ccatcggctt tagcgattgc cgccgcgccc agaccgattg
     6901 gccccgcccc gaccaccagc acctgctcgc cgggagcaat ggctgcgcga cgcaccgcat
     6961 gagcgctaat agcgaaaggt tcaatcaatg cggccgcctg cgggtcaata ccgtctgcgg
     7021 gcaaaatgtt cgccaccggc acgctcaaat actcactaaa accgccatcc tgatgcacgc
     7081 caatgactga aattttttca cagcagttgg tacgcccgct tttacacgcc gggcattgct
     7141 gacaggcaac ataagggatc acagcaactt gctgaccatt tttaagatca gcaatatttt
     7201 tacccagccc aacaatctcc ccacatattt catggcctaa aacccgtgga taactaaaaa
     7261 atggttgatt tccaccctag gcatgaatat cggtaccgca aatcccgaca gacttaattt
     7321 ttattaatgc ttcattgtca cccggaatag gtatctcgcg ttgtttccag actaattctt
     7381 tcggctgctg gcaaattaaa acattcatcg tagacatgat tcggctatcc attttttgtt
     7441 gccttagata tcaatgaaaa tgaaaaatgg cagcggaacg gaaaatctgt tttgtgaaaa
     7501 cacacgcata aaaatgtttt aaatcgggtt ttaatggact cacaaggagg aaaatatgag
     7561 tcgttcacaa aatttacgcc acaatgtgat taaccaggtc attgatgata tggcgcgggg
     7621 tcatatccct tccccacttc catcgcaaag tgcgctggcg gaaatgtaca acatcagccg
     7681 cactacagtg cgtcatattc tcagccaatt acgcgaatgc ggcgtcttaa cgcaggttgg
     7741 aaacgactat gttattgcgc gtaaacctga ccatgatgac ggtttcgcct gtactaccgc
     7801 ttcaatgagt gaacaaaaca aagtgtttga gcaggctttt ttcacgatga taaaccagcg
     7861 tcagttacgc cctagggaaa ctttttctga actgcaactg gctcgggccg cgggtgtcag
     7921 cccggtggtt gtaagagaat atcttttaaa attcggccgt tataatctta ttcaaagcga
     7981 aaagcgcggc cagtggagca tgaagcaatt cgatcaatcc tacgccgagc agctgtttga
     8041 gctacgcgaa atgctcgaaa cacattcact tcagcacttt ctcaacctgc ctgatcatga
     8101 tccccgctgg ctgcaggcga aaaccatgct agaacgtcat cggctactgc gtgacaacat
     8161 cggcaatagc tttcgcatgt tctcgcaact tgacagagat tttcactcct tactgctttc
     8221 agctgccgac aatatctttt ttgatcaatc gcttgagatc atctcagtca tctttcattt
     8281 tcactatcaa tgggacgaaa gcgatcttaa acagcgcaat atcattgccg tcgatgaaca
     8341 tatgaccatt ctcagcgctc tgatttgccg cagtgacctt gatgccaccc ttgcgctccg
     8401 taaccatttg aattcagcta aacaatcaat gattcgatcc attaatgaga acacgcgtta
     8461 tgctcattaa gtacggatta aaaacagcaa actattagcg ggtgcacggt tttatcaaaa
     8521 gataaatacc gggaaattcc cgcttaccta tgctcacaat cagtcgacta aaaatgtttt
     8581 tgtgagcggt agtaaagtcc taaaacttta acctgccgtt aatgtcaacc ttgcgtaatg
     8641 cacaattacg taaacaatta aaattataaa tacaggagtc cggcgtggaa aaagaaaata
     8701 tcaccctcga tccgcgttct tcatttactc catcttcgtc ggcagatatt cccgtgccac
     8761 cagatggatt agttcaacgc agtacccgaa ttaaacgcat tcaaaccacc gccatgttgt
     8821 tattattttt tgcggcggta atcaattatc tcgaccgcag ttcgctgtcg gtagcaaatt
     8881 taacgattcg tgaagaattg ggattaagtg ccaccgaaat cggcgctttg ctctccgtgt
     8941 tttcactcgc ttacggcatt gcgcaacttc cttgcggccc actattggat cgtaaaggcc
     9001 cacgcctgat gctgggactg gggatgttct tctggtcact gttccaggcc atgtctggca
     9061 tggtgcacag ctttacgcag ttcgtgttgg tgcgtatcgg tatggggatt ggtgaagcgc
     9121 cgatgaaccc atgcggtgta aaagtcatta acgactggtt caacatcaaa gagcgcggac
     9181 gcccgatggg cttcttcaac gcagcttcta ccattggcgt tgctgtaagc ccaccgattc
     9241 tggcggcaat gatgctggtg atgggctggc gcgggatgtt tattaccatt ggtgtactgg
     9301 ggatttttct cgccatcggc tggtatatgc tctatcgcaa ccgcgagcac gtagaactga
     9361 ctgccgttga acaagcttat ctcaatgcag gtagcgtcaa tgcccgccga gatccgctca
     9421 attttgccga atggcgcagc ctgttccgta accgcacaat gtggggaatg atgctcggat
     9481 tcagtggcat caactacact gcgtggctgt atctggcctg gcttcctggt tacctgcaaa
     9541 cagcctataa cctggattta aaaagcacag ggttgatggc ggctatccct ttcctgtttg
     9601 gggctgccgg gatgctggtc aacggttacg ttactgactg gctggtcaaa gggggaatgg
     9661 ctccgattaa aagccgtaag atctgcatta ttaccgggat gttctgttct gccgccttta
     9721 cgctgatagt accacaagcg acaacatcca tgatggcggt tctgctgatt ggcatggcac
     9781 tgttctgtat tcactttgcc ggaacatcct gctggggctt gatccacgtc gcagttgctt
     9841 ctcgcatgac tgcgtcggtg ggcagtatcc agaactttgc cagcttcatc tgcgcctctt
     9901 tcgcgccgat cattactggt tttattgttg atacaaccca ctcattccgt ctggcactaa
     9961 tcatttgcgg ttgcgtcacc gcagcgggag cactggcgta catcttcctg gttcgccagc
    10021 cgatcaacga cccgcgtaaa gattaaactc cctatgcccg ataacatttt gcttatcggg
    10081 catttttatg gcgattaaaa cgtttcccag ttctccccgc tatctgccac tgccatttta
    10141 cgcggcgtag ctggcgtcac ggtttttacc acagccgatg tttcacgctg ctgttgctgc
    10201 tgttgctgaa tccggaacac tgccacagct tcggtcaggc gactggcctg ctcttccagc
    10261 gcggcggcgg cagcggcaga ttcttccacc agcgcggcgt tctgttgagt tacccggtcc
    10321 atctcagcaa ccgctaagcc aacctgatcg ataccacggc tctgctcatc agaagcagaa
    10381 gctatttcgc ccataatgtc cgtcacgcgg gtcacagcac tgacaatctc cgccattgtt
    10441 tccccggcgc tttcgaccag cgtagagcca acatccactt tccccaccga gtcttcaatc
    10501 aggcttttaa tttcacgaac cgcctgggcg ctacgctggg ccagattacg cacttctccc
    10561 gcaaccaccg caaaaccacg tccttgctca cccgcacgcg ctgcttcaac cgccgcgttc
    10621 aaagccagaa tattggtctg gaaggtaatg ccgtcaatta cgctgataat atcggcgatt
    10681 ttctgcgaac tggtggagat atcgcgcata gtctgcacca cgttatctac cactttgccg
    10741 ccgcgttgcg ccgtttcaga agcacttaat gccagatggc tggcctggcg cgcattctcg
    10801 gcgttctgtt tcaccgttgc ggtcagttgc tccatgctgg ctgccgtctc ttccagcgaa
    10861 gcggcctgtt gctcggtgcg cgaagagaga tcattattgc cggtagcgat ttcgctggca
    10921 ccgctataga tggcattggc cccgttgcgc acatcaccga cggtacgcat cagctctccc
    10981 tgcatatggc gcaaactctc tgccagttgc cccatctcat tagagccatc cacctcaatc
    11041 ggtttcacca gatcgccgcc tgcaatatga cgaatgctgt caatcaggcg attcattggc
    11101 gctaccagcg aggctttaat accgaaccag acggcgaaga tgaccgccag tacgacgatc
    11161 atcacgccca ccagaatcca catcgcctgg ctgtaggagg cattgttatc gctgacggcg
    11221 atatcataga gccgatcgtt ttgctccatg taagccacat actgcttctc gaaaccgtcc
    11281 tgatatccct gggtcggctg atcaaagaac tcgttgattt tgcctgcacc taacagttgg
    11341 atcagctccg ccagcgcatt gtgataaata tcgtaattac gtttgatctc tgccgctgcg
    11401 gcggtgctct gacgcgggtc acgcggcaac gcttcgtaat ccgcccagtt tttttccgcc
    11461 tgtttcagcg aaatactggc actctgcatc agctcagcaa cggttgaacc gctaccaata
    11521 ttattctgat ccatcatgta gcggataccc gcgcggttga gggtgttacg cgtctgcaac
    11581 aacgcgaccc agctgccatt cagcgtgggt tgctgctggc gaatggtttg taaaacagtg
    11641 aaattttctt tgtcattctt taaggcatta aaaaacagac cgcctgatgt cagttgtaaa
    11701 aggccaaaaa cggccaaaac cagcagtaag ctggtcacga tcttgatacg ttttaacatg
    11761 gtttctcttt cctgtggacc gatgcaagat tttcggcctg gaaagcaaaa actttatgaa
    11821 aaggtgtaac ccgccatcca tggaaaaacc aggcgctggt tatcagatac taaaaagtta
    11881 ttcattatcg cccgggatgt cttcttgtcg ggaattgccc agagcaataa attcctctaa
    11941 cagatgggtt aactgttgca ttttctcggc agtaaactga gcttcaatct gccgataggc
    12001 ttcttcaatc tgtgtctgag cgcggttata cagcgcctgc ccctctttgg tcagcgagat
    12061 atagagcttc cgctgatcat tgatcggctt taatcgcaac actaaaccgt cgcgttccat
    12121 ccgcgtgagg atcccggtca ggcttgggcg caaaatgcag gcgcgatacg ccagatcgtg
    12181 aaaatccatt gatgggcttt ccgccagaat acgcacgatg cgccactgtt gctcagtcaa
    12241 attatgccgt ttaacgattg ggcgaaaata actcatcgcc gcttctcgcg cctggagcaa
    12301 cgcaatggtt agtgagtcgt gcattatctt tcccctgaaa aattcaaaat cattaatata
    12361 gaaacaatta atcatcacgt taccgggact gagctgcccc cgcaattctt cattttatca
    12421 aaattgatga tattttatta acttactgat atttaatatt ttattatctt taaatgtaaa
    12481 tagtttgtta attagatcac atttatatca cttacttttg ccaaactacg caatcgggaa
    12541 taaaagtaat tattaacata ttaatgatta agatgatcga atccgaggag tggtaatgaa
    12601 aggcactatc ttcgccgtag cgttgaacca tcgcagccaa cttgatgcct ggcaggaagc
    12661 gttccagcaa tctccctaca aagccccgcc taaaactgcg gtctggttta ttaaaccgcg
    12721 caatacggtg attggttgcg gtgaaccgat tccctttcca cagggtgaaa aagtactgag
    12781 cggtgcgact gttgcgctga ttgtggggaa aacggcgacg aaagtacgtg aagaagatgc
    12841 ggcagagtac atcgccggat atgcgctgga taacgacgtc agcctgccgg aagagagctt
    12901 ttaccgtccg gcaatcaaag caaaatgtcg tgatggattc tgccccattg gcgaaaccgt
    12961 ggctctcagc aatgtcgata atctgactat ctataccgag atcaacgggc gtcctgccga
    13021 tcactggaat accgccgatt tacaacgtaa cgccgcacag ctgctgagcg ccctgagcga
    13081 atttgccacg ctgaatccag gcgatgctat tctgctcggc acgccacagg cgcgcgtgga
    13141 aatacagcca agcgatcgcg tgcgtgttct cgcagaaggt ttcccgccgc tggaaaatcc
    13201 ggtagtggac gaacgtgaag tgaccacgcg caagagcttc ccaacgctgc cacacccgca
    13261 cggtacgctg tttgccctcg gcctgaacta cgccgaccac gccagcgaac tggaatttaa
    13321 gccaccggaa gagccgctgg tgttcctgaa agcgccgaat accctcactg gcgacaacca
    13381 gacctccgtg cgcccgaaca atattgaata catgcactac gaagcggagc tggtggtagt
    13441 tattggcaag caggcgcgta acgtcagcga agccgatgcc atggattatg tcgcgggcta
    13501 caccgtgtgt aacgactacg ccattcgcga ctatctggaa aactactacc gccctaacct
    13561 gcgggtcaaa agccgcgacg gactgacgcc gatgctttca accatcgtgc cgaaagaggc
    13621 gatcccggac ccgcataatc tgacccttcg caccttcgtc aacggcgagt tacgccagca
    13681 aggcaccacc gccgatctga tcttcagcgt gcccttcctg atcgcctact taagcgaatt
    13741 tatgaccctg aatccgggcg acatgatcgc caccggcaca ccaaaaggct tatctgacgt
    13801 ggtgcctggc gatgaagtag tggtggaagt agaaggcgtg ggccgcctgg tgaaccgaat
    13861 tgtgagtgag gaaacagcga aatgaaaaaa gtaaatcatt ggatcaacgg caaaaatgtt
    13921 gcaggtaacg actacttcca gactaccaat ccggcaacgg gtgaagtgct ggcggatgtg
    13981 gcctctggcg gtgaagcgga gatcaatcag gcggtagcgg cagcgaaaga ggcgttcccg
    14041 aaatgggctg atctgccgat gaaagagcgt gcgcgcctga tgcgccgtct gggcgatctg
    14101 atcgaccaga acgtgcctga gatcgccgcg atggaaaccg cggacacggg cctgccgatc
    14161 catcagacca aaaatgtgtt gatcccacgc gcttctcaca actttgaatt tttcgcggaa
    14221 gtctgccagc agatgaacgg caagacctat ccggttgacg acaagatgct caactacacg
    14281 ctggtgcaac cggtaggcgt ttgtgcgctg gcgtcgccgt ggaacgtgcc gtttatgacc
    14341 gccacctgga aggtcgcgcc gtgtctggcg ctgggcaata ccgcggtgct gaagatgtcg
    14401 gaactctccc cgctgaccgc cgaccgcctg ggtgagctgg cgctggaagc tggtattccg
    14461 gcgggcgtgc taaacgtggt acagggctac ggcgcaaccg caggcgatgc gctggtccgt
    14521 catcatgacg tgcgtgccgt gtcgttcacc ggcggtaccg ccaccgggcg caacatcatg
    14581 aaaaacgccg ggctgaaaaa atactccatg gagctgggcg gtaaatcacc ggtgctgatt
    14641 tttgaagatg ccgatattga gcgtgcgctg gacgccgccc tgttcaccat cttctcaatc
    14701 aacggcgaac gctgcaccgc cggttcgcgc atctttattc agcagagcat ctatccagaa
    14761 ttcgttaaac gctttgccga acgcgccaac cgtctgcgcg tgggcgatcc gaacgatccg
    14821 aatacccagg ttagggcgct tatcagtcag caacactggg aaaaagtctc cggctatatc
    14881 cgtctcggca ttgaagaagg cgcaaccctg ttggcgggcg gcccggataa accgtccgac
    14941 ctgcctgcgc acctgaaagg cggcaacttc ctgcgcccaa cggtgctggc ggacgtagat
    15001 aaccgtatgc gcgttgccca ggaagagatt ttcgggccgg tcgcctgcct gctgccgttt
    15061 aaagacgaag ccgaaggctt acgcctggca aacgacgtgg agtacggcct cgcgtcgtac
    15121 atctggacac aggatgtcag caaagtgtta cgcctggcgc gtggcattga agcaggcatg
    15181 gtgttcgtca acacccagaa cgtgcgcgac ctgcgccagc catttggcgg cgtaaaagcc
    15241 tccggcaccg ggcgtgaagg cggtgagtac agcttcgaag tgttcgcgga aatgaagaac
    15301 gtctgcattt ccatgggcga ccatccaatt ccgaaatggg gagtctgata tgggtaagtt
    15361 agcgttagcc gccaagatca cgcacgtacc gtcgatgtat ctctctgaac taccaggaaa
    15421 aaaccacggt tgtcgccagg gtgcgatcga cgggcataaa gagatcagca agcgttgccg
    15481 ggaaatgggc gtcgatacca ttatcgtttt cgatactcac tggctggtca acagtgctta
    15541 tcacatcaac tgtgcagacc attttgaagg cgtctacacc agcaacgagc tgccgcattt
    15601 tattcgcgac atgacctaca actacgaggg caacccggag ttggggcagc taattgccga
    15661 ggaagcctta aagctcggcg tgcgggcaaa agcgcacaat attcccagcc tgaaactgga
    15721 atacggcacg ctggtaccga tgcgctacat gaatgaagac aagcacttca aagtggtctc
    15781 catttcggct ttctgcacgg ttcacgattt tgccgacagc cgcaagctgg gcgaagcgat
    15841 tctgaaagcg atcgaacagt acgacggcac cgtggcggtc cttgccagcg gttcgttatc
    15901 gcaccgcttt attgacgatc agcgtgcgga agaagggatg aacagctaca cccgcgagtt
    15961 cgaccgccag atggacgagc gtgtggtgaa gctgtggcgc gaaggccagt tcaaagagtt
    16021 ttgcaatatg ctgccggagt acgccgacta ctgctacggc gaaggcaata tgcacgacac
    16081 ggtgatgctg ctggggatgc tcggctggga taaatacgac ggcaaggtgg agtttattac
    16141 cgagctgttc ccaagctctg gcaccggtca ggttaacgct gttttcccgc tgcccgcgta
    16201 aggacgtttt atgccgcact ttatcgtgga atgtactgaa aatattcgtg aagaagcccg
    16261 cttacctgag ctgtttgcca gtgttaacac tgcactggcg gcaacgggga tttttccgct
    16321 tgggggaata cgtagccggg cgcactggat cgatacctgg caaatggctg atggcaagca
    16381 cgattacgcc tttgtgcata tgacgctaaa aatcggcagc ggacgcagtc tggaaagccg
    16441 tcaggaagtg ggcgaaatgc tgtttgatct gattaaaacg cacttcgcct cactgatgga
    16501 gagccgttac ctagcgcttt catttgagat tgcagagctg cacccgacgt tgaatttcaa
    16561 acagaacaac gtacacgcgt tgtttaaata aatttgcacc agcttccagg ccggatgata
    16621 aggcgctgcc gcatccggca atgccacagg atatcgctat gttcgataaa cacacccaca
    16681 ccctgatcgc ccagcgtctg gatcaggcag aaaaacagcg cgaacagatc cgcgcgatct
    16741 cgctggatta cccggagatc accatcgaag acgcctacgc cgtgcagcgc gaatgggttc
    16801 gcctgaaaat cgccgaaagt cgcacgctga aaggccacaa aatcggtctg acctcgaaag
    16861 cgatgcaggc aagctcgcag atcagcgaac cggattacgg tgcactgctg gacgacatgt
    16921 tcttccacga tggcagcgat atcccgaccg atcgctttat cgtgccgcgc attgaagtgg
    16981 agctggcttt tgtgctggca aaaccgctgc gtgggccaaa ctgcacgctg ttcgacgttt
    17041 acaacgccac ggactatgtg atcccggcgc tggagctgat cgacgctcgc tgccacaaca
    17101 tcgatccgga aacccagcgc ccgcgcaaag tattcgacac catttccgat aacgccgcca
    17161 acgccggagt gatcctcgga ggtcgtccga tcaaaccaga cgaactggat ctgcgttgga
    17221 tctccgccct gatgtatcgc aatggcgtga ttgaagaaac cggcgtcgcc gctggtgtgc
    17281 tgaatcatcc ggcaaacggc gtggcctggc tggcgaacaa actcgcaccg tatgacgtac
    17341 aactggaaac cgggcaaatc attctcggtg gttcgttcac cctcccggtt ccggcgcgta
    17401 agggcgacac cttccacgtc gattacggca acatgggctc cattagctgc cgctttgttt
    17461 aaggagagaa cgatggaaaa cagttttaaa gcggcgctga aagcaggccg tccgcagatt
    17521 ggattatggc tggggctgag cagcagctac agcgcggagt tactggccgg accaggattc
    17581 ggctggttat tgatcgacgg tgagcacgcg ccgaataacg tgcaaaccgt gctcacccaa
    17641 ctacaggcga ttgcgcccta ccccagccag ccagtggtac gcccatcgtg gaacgatccg
    17701 gtgcaaatca aacaactgct ggacgtaggc acacaaacct tactggtgcc gatggtacaa
    17761 aacgccgacg aagcccgtaa agcggtacgc gccacccgtt atccccccgc cggtattcgc
    17821 ggtgtgggca gtgcgctggc tcgcgcctcg cgctggaatc gcattcctga ttacctgcaa
    17881 aaagccaacg atcaaatgtg cgtgctggtg cagatcgaaa cgcgtgaggc aatgaagaac
    17941 ttaccgcaga ttctggacgt ggaaggggtc gacggcgtgt ttatcggccc ggcggatctg
    18001 agcgccgata tgggttatgc cggtaatccg cagcacccgg aagtacaggc cgccattgag
    18061 caggcgatcg tgcagatccg cgaagcgggc aaagcgccgg ggatcctgat cgccaatgag
    18121 caactggcaa aacgctatct ggaactgggc gcgctgtttg tcgccgtcgg cgttgacacc
    18181 accctgctcg cccgcgccgc cgaagcgctg gcagcacggt ttggtaatag ttcttctatt
    18241 tcgatggcga agcaaaacaa caactctgtc tactaaacgt acgatgtgga gcgcatcatg
    18301 acgacctcta ccctgcaata taaagataat aaaactgttg aaattgaaca tcgtgtcatt
    18361 aataaattat ttcgtcgcct aatcattttt ctttttattc tgtttgtttt ttcgtttctt
    18421 gatcgcatca atataggatt tgccggactg acaatgggca aggatcttgg cctgacgtcc
    18481 acaatgtttg gcctcgccgc aacactcttt tatgtcacgt acgtcatgtg cgggatcccc
    18541 agtaatatga tgttagcgaa aattggcgct cgtcgatgga tcgctgggat catggtagtc
    18601 tggggaattg cttccacctg cacaatgttt gcaacaagcc ccgaaacact ctatctactg
    18661 cgtatgctgg ttggtattgc tgaggcgggt tttttacctg gtattttggt ttatctgacc
    18721 tggtggttcc cggcatatca tcgggccaga gccaatgcct tatttatgat agcgatgcca
    18781 gtcactatga tgctgggatt gatcctttca ggctacattc tggccttgga tggactgtgg
    18841 aatctaaaag gctggcaatg gctctttttg ctggaaggtc tgccatcggt cattcttggt
    18901 gtcgtcacct ggttttatct caacgacaca ccagaccagg cgacctggct tgatgaaaac
    18961 gaaaaacagg ctttaaaaac gatgattctc cgtgaacaag agagtgcatt tgcccatgca
    19021 tctgttccac gctcaacctt acgtgaagtc ctgaccccag ccatcatgct atatacactt
    19081 gcttattttt gcctgactaa tactctgagt gcgattaata tctggacgcc gcaaatccta
    19141 cagagtttca atactggcag tagcaacatc atgattggtt tgctggcggc catcccccaa
    19201 ttctgcacaa tttttggcat gatctggtgg agtcgtcgtt ccgaccgtct taaagaacgc
    19261 aaaaagcata cgattcttcc ctacctgttt gccgctgccg gatggttact cgcttctgcg
    19321 acagatcaca gcctcattca actgagcagc atcattatgg cctcaatggg atcatttacc
    19381 gcgatgacta tcttttggac aacaccggat caagttataa gcttgcaatc gcgtgccgtt
    19441 gcgatggctg tcatcaatgc tgtcggcaat gtaggttcag cagtcagtcc attacttatc
    19501 ggtattttga aagatgcaac cggcagtttt agctcaggtt tgtggtttgt agcaggattg
    19561 ctggtactgg gcgcgttagt catgacccga atccctatgg ggagtcggga acactctctt
    19621 tcatctgaac ttatcgcacc aaaaccgcaa taaggagtca tgatgtccag tgaagctatc
    19681 tttgaacatg gttatgaaaa tattgatttg gtccgggaat atgatgcccg ctacgctggt
    19741 gaagatgtgc attatgaaac atttgcccgg ttagcgaatt tctttggacg cgatatgcgc
    19801 cctcactggc acgatagata ttttcaactc cattatctgg tcactggacg cattacttta
    19861 caactggatg agcacttcta ttctgtgcgc gcaccgcttt ttgtactaac accaccatcg
    19921 gtgccacata ctttctttac tgatcctgat agcgatgggc atgttctgac cgtacgtcag
    19981 gagttaattt ggcccctggt agaaaagctc tggcctggta gtggagatgc cgcaatgcta
    20041 caaggtatct gtctgtcact ggagggaatg gaacatacac tggcggcatt aaatcattat
    20101 tggccgataa tatccctgga gtttcaacaa aataagcaag gacgggaaat tctattgcaa
    20161 tcactggcgc aaagtatttt tacaatccta ctgcgtgaag caccaccaaa tgatatttca
    20221 acatgcagcg tgcgcggcga gatgtgttta ttccagaagt ttaatcggct gattgacgaa
    20281 aattatcgcc aacatttaat ggtccctgaa tatgcaacaa tgctcggtat gagtgaatca
    20341 cggcttaccg agctgtgccg acgttttgcc aatcaatcgc cgaagagatt gattttcgaa
    20401 cgagtgagcc gggaagccag acggcttctg ctctatagcg agcaaagcat caaccagata
    20461 gcccttgatc ttggctacaa agatcccgcc tatttcgccc gttttttcaa ccgaatgatg
    20521 ggttgctcac caacacaatt ccgtggtcgc tgaccattta tcaacctgag agactcgaca
    20581 tgaaaatatc tttacgctca ctatttgtgg cgtgcctgat tgcgccagtg gcattcacct
    20641 acgcctccga accgcaataa atgaaaattg tactacttgg aacgaaaggt ggcccttctt
    20701 tattaaaagc tgaccgttta ccgatggcaa cggcacttat tattggcaat aaaacctggt
    20761 taattgatgc tggttacggg gccagcttac aactggtaaa aaataatatt ccactgcgcc
    20821 agattgataa tattttactc acccatttac acagtgatca tattctggac tatccatcag
    20881 tcttaatgaa tgcatgggct agcggtctgc aggacaaaac tattcatgta ttcggtccgc
    20941 caggcacccg agccatgacc gctgccagtt ggcaggtctt cgatcgggac atcacactgc
    21001 gtatggaaga agaaggaaaa cccgatcctc gcaaactcgt tacagctcaa gatgttggtg
    21061 aaggcgtagt ttataaagat gataccgtga ccgtgactgc attaaaggtc cctcattctc
    21121 ctttccctga cggtgaggct tttgcttatc gcttcgacac acaaggcaag cgaattgtct
    21181 tctcaggaga tacctcgtac tttcccccac tggcaacttt ctcagaaggc gctgatatac
    21241 ttgtgcatga ggccgtacat gagccctccg tcgctaagct ggcagacagt attggcaatg
    21301 gtaaaacact ggcaaaagcc attgcttctc accatacgac tattgaggat gtaggcaaaa
    21361 ttgccaatga agcaaaagtg aaaaaactca tccttagcca cctggtacca atcaccgttc
    21421 cggatgaaac atggaagcaa gaagcacaga aaactattca ggtcctatcg tcgttggtca
    21481 tgaaaatatg acgattacca ttccattcgc cccataaaaa attccgtccc cgttagccct
    21541 tgttagcggg gaatattatg atttgcctta ctgtaagttg atcacgatcg cagtttacta
    21601 tttaactcca ccttgctgtc gaaaagtacc aacgttcgcc tgaaacgtct cttcccgaca
    21661 accgcgttca acgcttcaat taaataacaa aaacaacaca caaaattaac aatcgtattc
    21721 cgattaatac tgtagaggtc gacatgaaac cagaagattt ccgcgccagt acccaacgtc
    21781 ctttcaccgg ggaagagtat ctgaaaagcc tgcaggatgg tcgcgagatc tatatctatg
    21841 gcgagcgagt gaaagacgtc aacactcatc cggcatttcg taatgcggca gcgtctgttg
    21901 cccaactgta cgacgcgctg cacaaaccgg agatgcagga ctctctgtgt tggaacaccg
    21961 acaccggcag cggcggctat acccataaat tcttccgcgt ggcgaaaagt gccgacgacc
    22021 tgcgccagca acgcgacgcc atcgctgaat ggtcacgcct gagctatggc tggatgggcc
    22081 gtaccccaga ctacaaagcc gctttcggtt gcgcactggg cgcgaatccg ggcttttacg
    22141 gtcagttcga gcagaacgcc cgtaactggt atacccgtat tcaggaaact ggcctctact
    22201 ttaaccacgc gattgttaac ccaccgatcg tcatttgccg accgataaag tgaaagacgt
    22261 ttacatcaag ctggaaaaag agactgacgc cgggattatc gtcagcggtg cgaaagtggt
    22321 tgccaccaac tcggcgctga ctcactacaa catgattggc ttcggctcgg cacaagtgat
    22381 gggcgaaaac ccggacttcg cactgatgtt cgttgcgcca atggatgccg atggcgtgaa
    22441 attaatctcc cgcgcctctt atgagat
//
LOCUS       DADLTY010000057        22412 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.057, whole genome shotgun sequence.
ACCESSION   DADLTY010000057 DADLTY010000000
VERSION     DADLTY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.057"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            82..2682
                     /locus_tag="JN334_001842"
     CDS             82..2682
                     /locus_tag="JN334_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HAY7438001.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2673..3656
                     /locus_tag="JN334_001843"
     CDS             2673..3656
                     /locus_tag="JN334_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7438002.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3754..4294
                     /locus_tag="JN334_001844"
                     /pseudo
     CDS             3754..4294
                     /locus_tag="JN334_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4302..4817
                     /locus_tag="JN334_001845"
     CDS             4302..4817
                     /locus_tag="JN334_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7438003.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4783..5520
                     /locus_tag="JN334_001846"
     CDS             4783..5520
                     /locus_tag="JN334_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAY7438004.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5631..6641
                     /gene="pyrD"
                     /locus_tag="JN334_001847"
     CDS             5631..6641
                     /gene="pyrD"
                     /locus_tag="JN334_001847"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAY7438005.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6815..7357
                     /gene="zapC"
                     /locus_tag="JN334_001848"
     CDS             6815..7357
                     /gene="zapC"
                     /locus_tag="JN334_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HAY7438006.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="JN334_001849"
     CDS             complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="JN334_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HAY7438007.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8707..10815
                     /gene="rlmKL"
                     /locus_tag="JN334_001850"
     CDS             8707..10815
                     /gene="rlmKL"
                     /locus_tag="JN334_001850"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAY7438008.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10827..12734
                     /locus_tag="JN334_001851"
     CDS             10827..12734
                     /locus_tag="JN334_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7438009.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12864..14117
                     /gene="pqiA"
                     /locus_tag="JN334_001852"
     CDS             12864..14117
                     /gene="pqiA"
                     /locus_tag="JN334_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HAY7438010.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14122..15762
                     /gene="pqiB"
                     /locus_tag="JN334_001853"
     CDS             14122..15762
                     /gene="pqiB"
                     /locus_tag="JN334_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HAY7438011.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15759..16322
                     /gene="pqiC"
                     /locus_tag="JN334_001854"
     CDS             15759..16322
                     /gene="pqiC"
                     /locus_tag="JN334_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAY7438012.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16578..16745
                     /gene="rmf"
                     /locus_tag="JN334_001855"
     CDS             16578..16745
                     /gene="rmf"
                     /locus_tag="JN334_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HAY7438013.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="JN334_001856"
     CDS             complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="JN334_001856"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HAY7438014.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17402..19162)
                     /locus_tag="JN334_001857"
     CDS             complement(17402..19162)
                     /locus_tag="JN334_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HAY7438015.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19347..19799
                     /gene="matP"
                     /locus_tag="JN334_001858"
     CDS             19347..19799
                     /gene="matP"
                     /locus_tag="JN334_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HAY7438016.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="JN334_001859"
     CDS             complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="JN334_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HAY7438017.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="JN334_001860"
     CDS             complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="JN334_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HAY7438018.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22005..>22412
                     /gene="sxy"
                     /locus_tag="JN334_001861"
     CDS             22005..>22412
                     /gene="sxy"
                     /locus_tag="JN334_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HAY7438019.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
BASE COUNT         5721 a         5259 c         5873 g         5559 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      661 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      721 ttacgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      781 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      841 tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
      901 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
      961 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1021 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1081 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1141 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1201 agtaatgaac agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg
     1261 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1321 ctgggattta accttggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1381 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1441 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1501 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1561 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1621 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1681 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1741 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1801 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1861 agtaatgata gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc
     1921 gataacgaag ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt
     1981 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc
     2041 gccagcatgg actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat
     2101 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2161 ggcattaccc tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca
     2221 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2281 gtcccctatg ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa
     2341 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     2401 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     2461 ataccgctgc gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt
     2521 attgatgatg atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta
     2581 cgctggggcg aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa
     2641 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     2701 tgttattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg
     2761 cttatatcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     2821 ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt
     2881 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     2941 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3001 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg
     3061 ttcctttcga aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg
     3121 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3181 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3241 gctacaacat acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt
     3301 acctgtctgg tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     3361 aggttgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     3421 gggccagaac ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag
     3481 ttaacgtcaa tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga
     3541 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg
     3601 ctggtgggac actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt
     3661 ctatccagtc agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3721 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3781 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3841 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     3901 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     3961 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4021 gacgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4081 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca
     4141 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     4201 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4261 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4321 ggattctgtt cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg
     4381 tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa
     4441 tcaatgtgca gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg
     4501 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca
     4561 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg
     4621 atgcagccag tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     4681 accaggcgag taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc
     4741 aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     4801 tacttcatta tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg
     4861 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     4921 ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt
     4981 attaacagca aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca
     5041 gcactattac tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg
     5101 cgcttgctga gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta
     5161 agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc
     5221 tcggtattta aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat
     5281 caacaattac gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac
     5341 tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg
     5401 ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc
     5461 tggcggacga taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga
     5521 aacaggttct gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa
     5581 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
     5641 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
     5701 agcaattacg ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg
     5761 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc
     5821 ttgataaaga cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga
     5881 tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg
     5941 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
     6001 tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata
     6061 aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg
     6121 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc
     6181 tgcaatatgg tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt
     6241 tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg
     6301 aagacgaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
     6361 caaccaatac cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg
     6421 gtggcttaag tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac
     6481 tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg
     6541 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
     6601 aaggtccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc
     6661 tttataagat aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc
     6721 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
     6781 gttgctgcgg ttttataggg taggggagag gcagatgcga attaaaccag acgataactg
     6841 gcgttggtat tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct
     6901 atttcgctca cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt
     6961 ttgtgttgat gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
     7021 atctaaagac caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa
     7081 accgcaaatg ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc
     7141 gggcgatgcg gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt
     7201 tgaatctggc gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg
     7261 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
     7321 gaatgttgac agcttcagcc tcgaacaggc agtctaacgc gtcagcttaa gggcagtctt
     7381 cggtacacag ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg
     7441 cgtgacttcg ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga
     7501 ataagggata cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc
     7561 acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt
     7621 ggcggtatca tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc
     7681 cacctcatcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
     7741 gacatcgccg ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc
     7801 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt
     7861 aaccacatca aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc
     7921 tgacgcgccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
     7981 acgttgttgg agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc
     8041 aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
     8101 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
     8161 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
     8221 agcaaaacga acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac
     8281 aggtgaagga gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc
     8341 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg
     8401 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc
     8461 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
     8521 ctataatgcg caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt
     8581 ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc
     8641 tccatgtcca gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt
     8701 gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact
     8761 gaactggaaa atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag
     8821 ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg
     8881 ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg
     8941 atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg
     9001 aatgacacca tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat
     9061 gctttcacac ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc
     9121 gttaacgtct ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt
     9181 ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca
     9241 gccgctattg tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt
     9301 ggttccggta cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg
     9361 caccgtgggc gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa
     9421 gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt
     9481 tacggttcgg acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg
     9541 gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg
     9601 ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc
     9661 gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt
     9721 ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt
     9781 gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac
     9841 gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc
     9901 ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac
     9961 cgcctgtatg acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg
    10021 gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt
    10081 ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg
    10141 ctgaaaaccc gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc
    10201 gagtttcttg aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc
    10261 gatactggtc tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa
    10321 ggcaaagatt tcctcaacct gttttcttat accggcagcg ccaccgtgca tgcgggatta
    10381 ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa
    10441 cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc
    10501 ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc
    10561 ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg
    10621 atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa
    10681 cgtggcttcc gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt
    10741 acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg
    10801 attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt
    10861 cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg
    10921 tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg
    10981 aacaagggct ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc
    11041 aacaggatcc gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg
    11101 aagaacaagc ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc
    11161 cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc
    11221 tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg
    11281 ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg
    11341 tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca
    11401 tcgactggct ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg
    11461 accgttcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg
    11521 tgacctatcc agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg
    11581 aagaactaca aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc
    11641 aggggatcaa agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc
    11701 gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg
    11761 ccagccgctc cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta
    11821 agcaactggt gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg
    11881 gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg
    11941 acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg
    12001 cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga
    12061 tggttaatgg caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga
    12121 aacgggcgat gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg
    12181 cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg
    12241 atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc
    12301 tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag
    12361 gcggcggtaa aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac
    12421 aatatgtggc actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag
    12481 cagaaactgt aaaacgcagc agtagcaaac taagctataa attgcagcgc gaactggagc
    12541 agctaccgca attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg
    12601 cggatgcttc cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg
    12661 ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa
    12721 aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat
    12781 tgcctgatga actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa
    12841 tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt
    12901 gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat
    12961 gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg
    13021 cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata
    13081 tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt
    13141 ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt
    13201 tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag
    13261 agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg
    13321 cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca
    13381 gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc
    13441 gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag
    13501 gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg
    13561 ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc
    13621 tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt
    13681 tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg
    13741 tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta
    13801 ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc
    13861 atggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc
    13921 gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag
    13981 gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga
    14041 caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag
    14101 agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg
    14161 aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt
    14221 cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa
    14281 ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa
    14341 agcgccacac tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt
    14401 atggaaaaat tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc
    14461 gaagggatta gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc
    14521 gcgaaaggca gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct
    14581 gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga
    14641 gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg
    14701 caaaaacgca atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc
    14761 agcaatgttc gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg
    14821 cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg
    14881 gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat
    14941 cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa
    15001 gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt
    15061 accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac
    15121 cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat
    15181 gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa
    15241 accggaaacc tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct
    15301 gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc
    15361 ttggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg
    15421 aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac
    15481 ctgcaaacga cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg
    15541 ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct
    15601 ggctccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga
    15661 gaactgcaac cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg
    15721 aaggacaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga
    15781 ttgcagcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt
    15841 tacctgtggt gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg
    15901 tagagcaggt cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg
    15961 atgtgaagta tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc
    16021 gcaacaccct ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc
    16081 ctctgggaag cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg
    16141 atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac
    16201 gtccgttccg tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc
    16261 tggccggtgt ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt
    16321 aattataaag atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt
    16381 ttctaacgtg cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg
    16441 tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg
    16501 tattgcttaa ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag
    16561 tatgagggaa acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac
    16621 gtggttatca ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga
    16681 gtcaaaggtc acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg
    16741 cctgattctg tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact
    16801 ctgtctgatg ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg
    16861 gcggtataga tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga
    16921 cgacgattaa caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt
    16981 acctgaccag tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc
    17041 agccagccga ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc
    17101 ataaccggat cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt
    17161 tctgcttcaa catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg
    17221 tctatcatca gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg
    17281 tgaccagagg caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct
    17341 gtaagcctta ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg
    17401 atcagttcgg aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt
    17461 cctgttgcga tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt
    17521 cgccatccca caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc
    17581 agatggtgaa tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc
    17641 gaacgttagc agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa
    17701 tagcaaagaa gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact
    17761 gatcgactga acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta
    17821 tcagggcgca gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct
    17881 caaatgtcag cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga
    17941 acgcctgcat gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt
    18001 cgatgtcggt gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc
    18061 caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc
    18121 gttcgccttc ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag
    18181 cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga
    18241 aggtgtcgcc atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc
    18301 taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc
    18361 aagcatccgc tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac
    18421 accactggct tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa
    18481 tagcctgctc tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc
    18541 ctaccagaat gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg
    18601 actcatcaaa cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc
    18661 acagcagagg ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct
    18721 gatgcaccag accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga
    18781 gttgctccgc ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat
    18841 ctgcactcac tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca
    18901 ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact
    18961 cagactcttc cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact
    19021 gctccagcgc aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa
    19081 tcaaatgtgg ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc
    19141 atgcaagttt cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta
    19201 taccgtaacc ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt
    19261 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    19321 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    19381 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca
    19441 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg
    19501 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    19561 agaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    19621 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg
    19681 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    19741 acgcgaataa aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac
    19801 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    19861 taccagacga taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg
    19921 atctctacgc gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg
    19981 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    20041 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    20101 ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg
    20161 tctttcggat ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    20221 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    20281 gtctgtactt ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa
    20341 cggtaggaaa cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg
    20401 tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg
    20461 tactcaacac cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg
    20521 ccaccaggta cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag
    20581 atgtccagat cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct
    20641 ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc
    20701 atttcaaaac caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg
    20761 ttttcgtggg tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc
    20821 agtttagcac cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa
    20881 ccagccagtg ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca
    20941 tccaaaatac gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga
    21001 tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta
    21061 aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa
    21121 gtcttgtata aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg
    21181 aaaccgcttc agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg
    21241 cgacaaaaaa agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat
    21301 ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa
    21361 ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa
    21421 atcatctgtc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat
    21481 tgactccact gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag
    21541 cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca
    21601 gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg
    21661 ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac
    21721 acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt
    21781 gtacataatc aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca
    21841 acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa
    21901 acataaatta atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt
    21961 taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct
    22021 ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat
    22081 cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg
    22141 gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg
    22201 tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg
    22261 aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag
    22321 cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt
    22381 cttttcatct ggaagcgatt ctcggggagg tg
//
LOCUS       DADLTY010000058        22279 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.058, whole genome shotgun sequence.
ACCESSION   DADLTY010000058 DADLTY010000000
VERSION     DADLTY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.058"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..1026)
                     /locus_tag="JN334_001862"
     CDS             complement(<1..1026)
                     /locus_tag="JN334_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7438020.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1128..2108)
                     /locus_tag="JN334_001863"
     CDS             complement(1128..2108)
                     /locus_tag="JN334_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HAY7438021.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2116..2826)
                     /locus_tag="JN334_001864"
     CDS             complement(2116..2826)
                     /locus_tag="JN334_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAY7438022.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3043..4866)
                     /gene="typA"
                     /locus_tag="JN334_001865"
     CDS             complement(3043..4866)
                     /gene="typA"
                     /locus_tag="JN334_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HAY7438023.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5239..6648
                     /gene="glnA"
                     /locus_tag="JN334_001866"
     CDS             5239..6648
                     /gene="glnA"
                     /locus_tag="JN334_001866"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HAY7438024.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6821..7870
                     /gene="glnL"
                     /locus_tag="JN334_001867"
     CDS             6821..7870
                     /gene="glnL"
                     /locus_tag="JN334_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HAY7438025.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7882..9291
                     /gene="glnG"
                     /locus_tag="JN334_001868"
     CDS             7882..9291
                     /gene="glnG"
                     /locus_tag="JN334_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HAY7438026.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9403..9513
                     /gene="yshB"
                     /locus_tag="JN334_001869"
     CDS             9403..9513
                     /gene="yshB"
                     /locus_tag="JN334_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HAY7438027.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9742..11115)
                     /gene="hemN"
                     /locus_tag="JN334_001870"
     CDS             complement(9742..11115)
                     /gene="hemN"
                     /locus_tag="JN334_001870"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAY7438028.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11304..11813)
                     /gene="yihI"
                     /locus_tag="JN334_001871"
     CDS             complement(11304..11813)
                     /gene="yihI"
                     /locus_tag="JN334_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HAY7438029.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12395..13027
                     /locus_tag="JN334_001872"
     CDS             12395..13027
                     /locus_tag="JN334_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAY7438030.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13409..16195)
                     /gene="polA"
                     /locus_tag="JN334_001873"
     CDS             complement(13409..16195)
                     /gene="polA"
                     /locus_tag="JN334_001873"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAY7438031.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16197..16439
                     /locus_tag="JN334_001874"
     CDS             16197..16439
                     /locus_tag="JN334_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438032.1"
                     /translation="MSVPVYQIMGKGCHRQDENMSFSPLFVKIFCRSAGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMMFVFVFHAVKNKSIPNN"
     gene            16559..17491
                     /locus_tag="JN334_001875"
     CDS             16559..17491
                     /locus_tag="JN334_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7438033.1"
                     /translation="MANLLNEFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17532..18962)
                     /locus_tag="JN334_001876"
     CDS             complement(17532..18962)
                     /locus_tag="JN334_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY7438034.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19117..19743)
                     /gene="dsbA"
                     /locus_tag="JN334_001877"
     CDS             complement(19117..19743)
                     /gene="dsbA"
                     /locus_tag="JN334_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HAY7438035.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19760..20746)
                     /gene="srkA"
                     /locus_tag="JN334_001878"
     CDS             complement(19760..20746)
                     /gene="srkA"
                     /locus_tag="JN334_001878"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HAY7438036.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20823..21092)
                     /locus_tag="JN334_001879"
     CDS             complement(20823..21092)
                     /locus_tag="JN334_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HAY7438037.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21162..21746
                     /gene="mobA"
                     /locus_tag="JN334_001880"
     CDS             21162..21746
                     /gene="mobA"
                     /locus_tag="JN334_001880"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HAY7438038.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21728..22255
                     /gene="mobB"
                     /locus_tag="JN334_001881"
     CDS             21728..22255
                     /gene="mobB"
                     /locus_tag="JN334_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAY7438039.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5512 a         5584 c         5334 g         5849 t
ORIGIN      
        1 tgcttcgccg ataggtgcaa aaaatacggc tcgctgtgta aagactatcg cgccaaagcc
       61 cagcgtacat gccatcccta aatagaccgg catactttcg tgcggcagca taatcaacag
      121 tacgagcgca gcggtactga tgataaaggt gtagcataga tatttactcg gcgatttcag
      181 gatcttatct gaaatcatgc caccaatcgg tccaccaatc attttcagac agtattggtt
      241 aatgatgccg taagccccca ccagcgcaac gggcaataga tagatgtttt tcaggaatgg
      301 aatgaagaat gtcaggccac agtaaaccgc gtaaacgaag aagacgttga aagcgataag
      361 ccagatggtt ttgtctctca gcactgaggt catactggtg ttttgcgatg ctccgtcttc
      421 ttttttcacc tcaacggacg gtgcctcttc tttgtcattc aggacaaaga aaataataat
      481 gcctacggca atcacaataa gggaatagaa ccagatgcct gctttaaaac ctaataagcc
      541 actgccaaac caggtaaata ctgccaacgc agaaaatgcc accacggtat cgacaatgcc
      601 acgccctgtt tcgaagaagc caaacaaccg accttgttgt tcgctattgc ccaatcgact
      661 taccgacttg agcaagaccg gccagttcat catgtcgcaa gtaacgccaa atagcgccca
      721 gacaaagagg atgccccaat accccggcat tgtcgtcaaa taaacaccca gtaatcctgt
      781 cgcaatgagt gagaatgaca tggtgtatct gcgcggtagt ttatcggcaa aataaataga
      841 cagaaaaaag cccactgtgg tgacaaatga gtttaccgac atagcattac caatttgacc
      901 attggtcaaa tggaaatatt cctgcatcgg gatataaaac gcatctttca gcgacggtaa
      961 tttataaatt gtaccgccac acagtgttaa tagactaaat aacgcccatt tctttttcgt
     1021 gagcataaca tcccctaagc gatttttaaa atcactgttt taaaggcaat aagataccga
     1081 ccattcatat ggaatggttt tattaactgc gtatgagtta aaacttatta gtggttcaga
     1141 agatggtttg cctcttgttt gatttgttgt agtacgttac gcacatgatt tatctgatta
     1201 attgcatctt ctttttcttt acgtaaccgc gcatccagta aatgattttc tggtaggggt
     1261 gtttcactca tctggcgata agggatccac ggattatcat cttcgtcttc aaagcggaac
     1321 gccgacgcat agtaatcaac ctcttcttcc aggccatatg ccgtcacctg cggctcatca
     1381 tcgccaaggc agataaggtg cgttaataac gctttgaatg gcatatcacc ctgacctgaa
     1441 atacaggctt tatgacccag gccaccctgt tctttaacga tcaaggcatc tttaatatgg
     1501 acctgggtaa tatgcggtgc catcgttttt aaggcgtcga tgggatgctc atttgcattg
     1561 atcatgttag caaaatcaaa cagtaaggaa agagactcca tctcactttc tttgaccagc
     1621 gacaccagct catgactttt taaatcttca tgctgctcaa tcgtaaaagt caggccgctg
     1681 tcctgatacg tttcccgtac ataggcaatg tcgttagcga taatcgataa tacgtcgcgc
     1741 aaattacctt cataacatgg ataaaaacgt acggacgttg ccccagtttt caacgcgatg
     1801 gcgacggctt cgtcaataga tgccttatct gaagcgctgg tttcaatatg aatatcaagg
     1861 ttcagacggc gggctttatc cccaaaggca gagagttctt tatcgtccat attaccaaga
     1921 gaaaaacgct cgccatccag aacatggatt ttgacgccac gtagattatt ttcacttgcg
     1981 atatcaagta aatcagcagg caacactctt tccagccgca tatttaaatg aaaggcataa
     2041 gcatgtaaat aaagaggaag agtgtcgaca cgttgtagaa tctttcttgc gttatttatt
     2101 gtcaccataa aacacctaca gattaatgat gaggtctacg gcctcaaatc gccaatattc
     2161 aatatctatc tggacaatat tgccttgctg gtcggcgcga tgcttttcaa ttaagatcgc
     2221 tggcataccc gcagaaccgc caagatactt gctgatatcg cccggcattc tgacgggttt
     2281 gaaaatcaat ttttttacta ccgtctcttt ttggtagcac ttttcccaga ctgcagaaaa
     2341 tgagtggttt tcaagttgtt caataaaacc cggagcaact tcaggattaa tatatgtttc
     2401 gtgatagaaa actttatgtc cttccagcgc cccccagccg gtgatgcgat agagttcagt
     2461 tgatggcgtc actgcaatca gtggcgcgag cgtctcggga atatcgctgg tacggttttt
     2521 ctcggtaaac ccccaagaag gctctcgccc ttgctcaatt gcggcccgct gaaagctcgc
     2581 cgacagctcc ggactgtaat taaatcgcgg ctgggtcacg aaccacccct tacgatcctt
     2641 acggaagatt ttggattctg cctcgaggtt caacaacgcc tggcgcagcg tcatacgttt
     2701 aatgcccagc agttctccca gctctctttc tgaagggagt tttccacccg gcgtggtgat
     2761 gccatctttc aaccaccgat ccagtttctc ttttgcattt tctacggtgg attgattctc
     2821 cgccattgtt taatcctttt ggtttaaacc agatgaagca tcttacgctt acgccaaaaa
     2881 agaaagcaat taacggtgta tgactttgca gataattgtg acaaagctct ctattgacgt
     2941 aatcacagac ttataaggga gatactcgca ttggtgtgcg ctcacgacag gtaaaaaaaa
     3001 acctgccatc gctggcaggt tttttatgac taaaaaacga aattaatcgt ctttcggtgc
     3061 gcggttggcg cggcgacgat cgttttccgt caggtgacgt ttacgaatac ggatagaggt
     3121 cggagtcact tctaccagtt cgtcatcatc gatgaactcc agagcttgtt ccagagtcat
     3181 gcggataggc ggaaccagaa caacagcttc gtcagtaccg gaagcacgca tgttggtcag
     3241 tttcttaccg gtcaggcagt ttacagtcag gtcgttagag cggctatgaa taccgataat
     3301 ctgaccttcg taaacttctg caccgtgacc gaggaacagc ttaccgcgat cctgcagacc
     3361 gaacagcgcg aacgcgacag ctttaccctg accgttagag atcagtacac cgttctgacg
     3421 ctgacccact tcgcccggac gtacgtcgtc gtagtggctg aaggtggagt acagcagacc
     3481 agtaccggaa gtcatggtca tgaactcaga acggaagcca atcagaccac ggcttgggat
     3541 cacgtagtcg agacgtacgc ggcctttacc gtctggattc atgtttttca ggtcgccttt
     3601 acgttcgccc agcgcctgca tcacagaacc ctgatgctgt tcttcaacgt ccagcgtcac
     3661 gttttcatac ggctcttgtt tacgaccgtc tatttcacgg aagattactt tcggacggga
     3721 taccgccagt tcgaaacctt cacgacgcat gttttcgata agaacagaga ggtgcagttc
     3781 accacgacca gaaacgcgga acgcatcggc gtcttcggtt tcttctacgc gcagcgcaac
     3841 gttgtgtacc agttctttgt tcagacgatc gaggatctga cgagacgtta cgaacttacc
     3901 ttctttaccg cagaacggcg aggtgttaac gcagaagaac atagaaacgg tcggctcatc
     3961 aacggagagt gccggcagcg cttcaacgtt ttgcgtgtcg caaacggtgt cagaaatgtt
     4021 cagttcgccc aggcccgtga tcgccacgat atcgccagct tccgccagat cggtttcgat
     4081 acgttccaga ccgaggtggc ccagcacttt accgactttc gcgttacggg ttttgccttc
     4141 gctatcgatg atagtaacct gctggttcgg cttcacttta ccacgcttga tgcggccaat
     4201 gccgataacg ccaacatagc tgttgtaatc gagctgagaa atctgcatct ggaacggacc
     4261 gtcaaggtca acgtccggcg caggaacgtg gtcaacaatc gcctgataca gcggggtcat
     4321 gtcttccgcc atatcttcgt ggtccagacc cgcgataccg ttcagcgcag aagcgtaaac
     4381 gatcgggaag tccagctgct cgtcggtcgc gtcgaggtta acgaacagat cgaatacctg
     4441 atccacaacc caatcaggac gcgcgccagg gcggtcaact ttgttgataa caacaatcgg
     4501 cttcaggccg taagcaaacg cttttttggt tacgaagcgc gtttgcggca tcgggccgtc
     4561 aaatgcgtca accaccagca gcactgagtc taccatggac attacacgtt caacttcacc
     4621 accgaagtcg gcgtgccccg gggtatcaac gatgttgata cggtaatcat tccatttgat
     4681 agcggtgttt ttcgcgagga tggtaatccc acgctctttc tccaaatcgt tggagtccat
     4741 cacgcgctct tgggtttcgg cacgagagtc gaacgtaccg gattgttgga gcagcttgtc
     4801 taccagggtg gttttaccat ggtctacgtg cgcgatgatg gcgatattac gcaatttttc
     4861 gatcacaact ttgcctcagg cattagaaat agcgcgttat tgtacacgga ttaatcgcac
     4921 tacaaaacag gatcacaaac atcctccgca aacaagtatt gcagagtccc tttgtgatcg
     4981 ctttcacgga gcataaaaag ggttatccaa aggtcattgc accaatatgg tgcttaatgt
     5041 ttccattgaa gcactatatt ggtgcaacat tcacatcatg gtgcagccct tttgcacgat
     5101 gttaagcatg ataacgcctt ttaggggcaa tttaaaagtt ggcacagatt tcgctttatc
     5161 ttttttacgg cgacacggcc aaaataattg cagatttcgt taccacgacg acaatgacca
     5221 atccaggaga gttaaagtat gtccgctgaa cacgtactga cgatgctgaa cgagcacgaa
     5281 gtgaagtttg ttgatttgcg cttcaccgat accaaaggta aagaacaaca cgtcactatc
     5341 cctgctcatc aggtgaatgc tgaattcttc gaagaaggca aaatgtttga cggctcctcg
     5401 attggcggct ggaaaggcat taacgaatcc gacatggtgc tgatgccaga cgcatccact
     5461 gcagtgattg acccgttctt cgccgactcc accctgatta tccgttgcga catccttgaa
     5521 cctggcaccc tgcaaggcta tgaccgtgac ccgcgctcta ttgcgaagcg cgccgaagat
     5581 tacctgcgtt ccactggcat tgccgacacc gtactgttcg ggccagaacc tgaattcttc
     5641 ctgttcgatg acatccgttt cggatcatct atctccggtt cccacgttgc tatcgacgat
     5701 atcgaaggcg catggaactc ctccacccaa tacgaaggtg gtaacaaagg tcaccgtccg
     5761 gcagtgaaag gcggttactt cccggttccg ccggtagact ctgctcagga tattcgttct
     5821 gaaatgtgtc tggtgatgga acagatgggt ctggtggttg aagcccatca ccacgaagta
     5881 gcgactgctg gtcagaacga agtggctacc cgcttcaata ccatgaccaa aaaagctgac
     5941 gaaattcaga tctacaaata tgtcgtgcat aacgtagcgc accgcttcgg taaaaccgcg
     6001 acctttatgc caaaaccgat gttcggtgat aacggctccg gtatgcactg ccacatgtct
     6061 ctgtctaaaa acggcgttaa cctgttcgca ggcgacaaat acgcaggtct gtctgagcag
     6121 gcgctgtact acattggcgg cgtaatcaaa cacgctaaag cgattaacgc cctggcaaac
     6181 ccgaccacca actcttataa gcgtctggtc ccgggctacg aagcaccggt aatgctggct
     6241 tactctgcgc gtaaccgttc tgcgtctatc cgtattccgg tggtttcttc tccgaaagca
     6301 cgtcgtatcg aagtacgttt cccagatccg gcagctaacc cgtacctgtg ctttgctgcc
     6361 ctgctgatgg ccggtcttga tggtatcaag aacaagatcc atccgggcga agccatggac
     6421 aaaaacctgt atgacctgcc gccagaagaa gcgaaagaga tcccacaggt tgcaggctct
     6481 ctggaagaag cactgaacga actggatctg gaccgcgagt tcctgaaagc cggtggcgtg
     6541 ttcacggatg aagcgatcga tgcgtacatc gctctgcgtc gcgaagaaga tgaccgcgtg
     6601 cgtatgactc cgcatccggt agagtttgag ctgtactaca gcgtctaata gttgaagttg
     6661 tactacccgg cgcacaacgc cgggatttag ttgccgtgga aactttcagc ccatctctgc
     6721 atgggctttt ttctccgtca attctctgat gcttcgcgct ttttatccgt aaaaagctat
     6781 aatgcactaa aatggtgcaa cctgttcagg agactgcttt atggcaacag gcacgcagcc
     6841 cgatgctggg cagatcctca actcgctgat taacagtatt ttgttaattg atgacaatct
     6901 ggcgatccat tacgccaacc ccgccgcgca acaactgctc gcccaaagct cccgcaaatt
     6961 gtttggtacg ccgttaccgg aactgttgag ctacttctca ttaaatatcg agctgatgca
     7021 agaaagtctg gaggcggggc aaggctttac cgataacgaa gtgacgctgg tcatcgatgg
     7081 tcgctcgcat atcctttctg tgacggccca gcgtatgccg gacggcatga tcctgctgga
     7141 gatggcaccg atggataacc agcgccgctt aagccaggaa caactacaac atgctcaaca
     7201 agtagctgcc cgtgatttag tgcgcggcct ggcacatgag attaaaaatc cgcttggcgg
     7261 tttacgtggc gcggcgcagt tactcagcaa agcgttacct gacccatcac tactcgaata
     7321 taccaaagtg attatcgaac aggcggaccg gctgcgaaat ttggtcgacc gtctgttggg
     7381 gccgcagctg cccggtacgc gcgttaccga aagtattcac aaagtggctg aacgcgtggt
     7441 aacgctggtg tcgatggaac tgccggacaa cgtgcggttg attcgtgatt acgatcccag
     7501 cctgccggaa ctggcgcacg acccggatca aattgaacag gtcttgctga atattgtgcg
     7561 caatgcgcta caggcgctgg ggccggaagg cggtgaaatc attctgcgta cccgcaccgc
     7621 gtttcaactg accttacacg gcgagcgcta ccggctggcg gcgcggattg atgtggaaga
     7681 taacgggccg ggcattccgc ctcatttgca ggatacgctg ttttacccga tggtcagcgg
     7741 ccgcgaaggt ggcaccgggc ttggcttatc catcgcccgt aatttgattg atcagcattc
     7801 aggcaaaatt gaatttacca gttggccagg tcataccgag ttctcggttt acctgcctat
     7861 caggaaataa aggtgacgtt tatgcaacga gggatagtct gggtagtcga tgacgatagt
     7921 tccatccgtt gggtgcttga acgtgcgctc gctggagcgg gtttaacctg tacgacattt
     7981 gagaacggcg cggaagtact ggaggcgctg gcgagcaaaa cgccggatgt gctgctttca
     8041 gatatccgta tgccgggaat ggacgggctg gcgctgctca agcagattaa acagcgccat
     8101 ccaatgcttc cggtcatcat tatgaccgca cattccgatc tggatgctgc cgtcagcgcc
     8161 tatcaacaag gggcgtttga ttatctgccc aaaccgtttg atatcgacga agcagtggcg
     8221 ctggttgagc gcgctatcag tcattaccag gaacagcagc agccgcgtaa tattcagctt
     8281 aacggcccaa cgaccgatat catcggcgaa gcgcaagcca tgcaggacgt gttccgtatt
     8341 atcggtcggc tttcgcgttc ttctattagc gtgctgatta acggcgaatc cggcaccggt
     8401 aaagaactgg tcgctcatgc cctgcatcgc cacagtccgc gcaccaaagc gccgtttatc
     8461 gcgctgaata tggcagctat cccaaaagat ttgatcgaat cagaactgtt tggccacgag
     8521 aaaggcgcgt ttactggcgc gaataccatt cgtcaggggc gttttgaaca ggccgatggc
     8581 ggcacgttat ttctcgacga aattggtgat atgccgctgg atgtgcagac gcgtttgctg
     8641 cgcgtgctgg cagacggtca gttttatcgc gttggcggct atgcgccggt gaaagtggat
     8701 gtgcggatta tcgctgccac tcaccagaat cttgaacagc gggtgcagga aggtaagttc
     8761 cgtgaggatc tgttccaccg cctgaacgtt atccgcgttc atctgccgcc gctgcgcgaa
     8821 cgtcgggaag atattccccg tctggcgcgc cattttttac aggttgccgc gcgcgaactg
     8881 ggcgtagaag cgaagttgct gcatccggaa accgaagctg ctctgacgcg tctggcgtgg
     8941 ccaggcaacg tgcgccagct ggaaaacacc tgccgctggc taacggtgat ggccgccggg
     9001 caggaagtgt tgattcagga tttgcccggc gaactgtttg aatcaacggt tgcggagagt
     9061 acttcgcaaa tgcaaccgga cagttgggcg acacttttag cgcagtgggc agacagagcg
     9121 ctgcgttccg gtcatcaaaa tctgctttcc gaagcgcagc cagagctgga gcggacgtta
     9181 ctgacgaccg cgttgcgaca tacgcagggg cataaacagg aagcggcgcg gctactcggc
     9241 tggggccgca acactctgac gcgtaagtta aaagagctgg ggatggagtg attcacggct
     9301 tgtgtgtaaa gattgattat tgagcgcaaa ttgctggtat tttgcgcttt actgttccga
     9361 taagttcagt atgatcttgc ccggaaaacg gggagagtca ttatgctgga atcaataatt
     9421 aatctggtat cgagtggcgc agttgacagc cacacaccgc aaactgccgt tgctgccgtg
     9481 ctgtgtgccg cgatgattgg gctgtttagc tgaagctcgt tgtttatgcc ggatgcggag
     9541 aaagtcgtag gcctgataag acgcgaccag cgtcgcatca ggcatcatgc tcagatgcct
     9601 gatgcggcgt aaacgcctta tccggcctac aaaagcaata atttcaacag gttttgttgc
     9661 ccagcgcatc aggcaatctt gttctcagca cattgatggc gctttcgttt tctactttgt
     9721 aaacgaagcg ccattcactc tttaaatcac ccgtgagaac tgctgcatcc gcgctttctg
     9781 gcgcagatag gtatcaaagc acatgcaaat gttgcggatc agcaagcgac ctttcgccgt
     9841 cacctgtatt cccttctcat ccacatccac cagcccatct tttgctaacg gggcgagcag
     9901 cttgagatct tccgcaaagt aatcagcgaa gtgcaaatcc cactgtttct caagaggggc
     9961 gtaatccaga cggaagttgc agatgagcga cttaatcaca tcgcggcgaa tacagtcatc
    10021 acgcgttagc gcaataccac gccacagcgc attgccttgt tcatacactt gctgatagta
    10081 ctgcttcaac tctttctggt tctgcgcgta gcagtcgcca atcatgctga tggcggaaac
    10141 gcccatcccc agcagatcgg tatcgccctg agtggtgtag ccctggaagt tacgatgcag
    10201 cacgccttca cgctgggcca ccgccagctc gtcatcagga cgggcaaagt gatccatacc
    10261 gataaactga tagcccgatt gcgtcaggaa ggcgatggtt tcctgcagga tatcgagttt
    10321 ttgctgcgga ctcggcaggt cagcatcttt gattttgcgc tgagcagcaa aaatggtcgg
    10381 cagatgcgcg tagttaaaaa cgctcagacg atcggggttc agctccgcca cacgtttcag
    10441 ggtaaaggcg aaactctccg gcgtctgttt cggcaggccg taaatcaggt cgatgttggt
    10501 ggaggtaaaa ccaatctcac gcgcatggtt aagcagtgca aagatgaact cttcatcctg
    10561 ctcgcggtta accagacgtt gcacttcttt gttgaagtcc tgcacgccca tgctcaggcg
    10621 attaaagcct tcggcgcgta aatgatcgag tacatccagt tcgatttccc gcggatcgac
    10681 ttcgatcgaa atctccgcat cggtattgaa atggaagttt tcgcgcagca gcttcatcag
    10741 acggctgatt tgcgctttat tcagatacgt cggcgttccg ccgccccagt gcaattggct
    10801 gacgtgacgc ccggcgaaca gcggtgcacg atggacgatt tcttgctcca gcgcgtccag
    10861 atactgatcg gccttgtgct gctggcgagt aacaatctta ttgcaaccgc agaagtaaca
    10921 aagcttatgg cagaacggga tatgtacgta gagagataat ggacgctcag gatagcgcgc
    10981 cacggcttgt aaaaacgcct gttcgccgaa gtcttctgaa aactccagcg cggtcgggta
    11041 cgaggtgtat cgtggcccgg aatagttata tttctggatc agggccagat cccagtcgat
    11101 ttgctgtaca gacatgctca ctccttccga tggcgtctct ggcggctacg gcgtaccggc
    11161 ggatgcccgt ttcgggtcat cagccggttg cgcagccact tttgtcgccg cgacaaccgc
    11221 tgtagtttaa cgaataacca caccagataa catataaccg gaagggttat cagcagaacg
    11281 ggaaggccca cggcggcaat ccgttagttg ccccgcagca gacgcatcat gtcttcttgc
    11341 ttctcgtctt cttcctcttc ttcgtcatcg tcataagaga ggccgagttt ctgcatcaac
    11401 tcatcaatac gatccagttt agcatccacc caggattgct cttcggcgct cagggtttcg
    11461 cctgcttcca gacgttccag cagcgcatcc agacgctcat ccgtttccag taattccaac
    11521 tccgcctgcg gtgaaagcat aggtttctca ctcttcggtt tgtgctgttt ggtgactttt
    11581 tcagtcactc ccaatggaat aggggtttta ctgccaatac gtggatcttt tggtgcgttc
    11641 tggcctttgc tgcctgacgt ggtgtttccg cccgctgcgc ggctgcccgg cgcatgacca
    11701 cgacgttttt tctgacgctt acggtcacga gcttcctgat ccagctcctc gcgtgttttt
    11761 cgacgcgctt ttgcatgacc tttgctgcgt gagtttgaag atgatggttt cataatggtt
    11821 cgtcttttag ccgttttatt cagtatagat ttgcggcgga atctaacaga aagcaagcaa
    11881 agaaaaaagg cgacagatta ctctgtcgcc ttttttcctg actcataacc cttaacgggt
    11941 cttacaatcc ttgcaggccg ccaacacacc ctgtttttcc attagccgga acaatccgtg
    12001 ttgattccat ttccgtttaa ttacgtctcc ggacgtttgt cttcctgaca atcctctgtc
    12061 ttcgcctcct ggcgctcctg accctcatcc tgagtcattg ttcctgttag cgtcctcgct
    12121 ctatgggtgc tactttacgc ctttgcttta aacaaacaat caaccgccat cagtatttgc
    12181 acattttcag agttttatat aaaagcaatt cactgattta tatagcttat ttgacatgca
    12241 tcgcagaatt ttctctgcta tttcgcataa agactatcgg caatgtctca caaagcgtat
    12301 ggcttttact tacaacgctc acgccgaaaa atccgccttt tattatggtt caggtataat
    12361 cgccacaaag ctttgactga cggagacgac cgctttgact aatttgaatt atcaacagac
    12421 gcattttgtg atgagtgcgc ctgatattcg ccacctacct tccgataccg gaattgaagt
    12481 ggcttttgca ggccgttcca acgcaggtaa atccagcgcg ctgaacacgc tgactaacca
    12541 gaaaagcctg gctcgtacct caaaaacccc agggcgcacc cagcttatca acctgtttga
    12601 agtggctgac ggcaagcgtc tggttgactt gcctgggtac ggttatgcgg aagtcccgga
    12661 agagatgaag cgcaaatggc agcgtgcgct cggcgaatac ctcgaaaaac gtcagagcct
    12721 gcaaggtctg gtggtgctaa tggatattcg ccatccgctg aaagatttgg atcagcagat
    12781 gattgagtgg gcggtagaca gcaatatcgc cgttctggtg ctgctgacca aagcggacaa
    12841 actggcaagc ggcgcacgta aagcgctatt gaatatggtg cgtgaagctg tactggcgtt
    12901 taacggtgat gtgcaggttg aaacgttttc ttcgttgaag aaacaaggcg tagacaagct
    12961 gaggcagaaa ctggatacct ggtttagcga gatgcagcct gtagaagaaa cgcaggacgg
    13021 cgaataattt tcttgcctta atgcttgtgc cggatgtggc gtatccggcc cgtaaattca
    13081 atagattaca attctaccgt tggcctgata aacatagcgc atcaggcatt acggatcttt
    13141 tctttcgccc aataaaaaac gccccagtca ttactgactg gggcggctaa aatattcagc
    13201 caaatccgat tacgtgaagt aaaaggtctg aaagatagaa catcttacct ctgtacccta
    13261 cgcgactaac tttactcttt tttgttcagt tcagaaagca ctttttgtaa ttttttttca
    13321 gttgttgcat aggaatttct aatagccatc acaaaacgtg acagcttatg ttgcttactt
    13381 acgaaaaaag ggcatgttca ggcgaatctt agtgcgcctg atcccagttt tcgccactcc
    13441 ccacttccac cagcaacggc acatccagac gggtacagtt ttccatcagt tgatgaatct
    13501 gcttcgcgac ggcatcaaca tcatctttat gaacttcaaa taccagttca tcgtgtacct
    13561 gcatgatcat acgtacacgc ggttgctcag cctgtaacca cgcatcaacg gcaatcatcg
    13621 cccgtttgat aatgtcggcg gcggttccct gcattggcgc gttaatggct gcacgttcag
    13681 ccgctgcacg acgagcacca ttgctggatt tgatatccgg cagatacaga cggcgtccgt
    13741 ccagcgtttc aacgtagccc agctctttcg cctgagcacg ggtgcgttcc atatactcca
    13801 gcacgccagg gtagcgttcg aagtaaaggt ccatgtactt ctgcgcttct ttacgtggaa
    13861 tgttcaattg ccgcgccaga ccgaaagcac tcatgccata aatcagacca aagttgatcg
    13921 ctttcgcgct acggcgttgc tcgctggtga cggtttccag tggcaaacca aacacttctg
    13981 ccgccgttgc ccggtggata tcttttcctt ccgcgaatgc ggtcagcaag cctttatcac
    14041 gcgagagatg cgccataatg cgcagttcaa tctgcgagta gtccgctgag acaatcacat
    14101 aatcctctgg cgcaataaac gcctggcgga tacgacgacc ttcttcgtta cgcaccggaa
    14161 tgttttgcag gttaggatcg gttgacgata aacgtcccgt tgcagttact gcctggtgat
    14221 aagaggtatg cacacgcccg gttttcgggt tgatcatcag cggcagcttg tcggtgtaag
    14281 tcgacttcag cttcgccaga ccacgatact ccagaatcac ttttggcaac ggatagtcca
    14341 gcgccagttc ttccagtacc tcttccgacg ttgacggcgc gccacccggc gttttcttca
    14401 gcggtttaat accctgtttt tcaaacagaa tggtttgcaa ctgcttggtg gaagaaaggt
    14461 taaattcttc acctgcaatt tcatgcgctt tcttttccag ctcagccaga cgcaaggtga
    14521 gctcttcaga atgattgtgc agcactttcg gatcgatctt cacaccgtta cgttcaatgc
    14581 gtgaaagcac cggcaccagc ggcatttcga tattctcgaa gacgttcaac ggccctttgt
    14641 gtttctgcag atccggccac attttcagat gcaactgcaa ggtgacatct gcatcttcgg
    14701 cggcgtaacg tccggcctct tcgagggcaa tctggttaaa ggtcagttga tttttgcctt
    14761 tacccgcaat ctcttcaaaa gtgatggttt tgtgcttcaa ccaacgttcc gcgaggctgt
    14821 ccatatcgtg acgcccggca acgctattga gaatgtagga ctccagcatg gtatcaaacg
    14881 caatcccacg cagttcaatg ccgtaattcg ccagaatacc gcgatcgtat ttcaggtttt
    14941 gcccgacctt cagcgccttt tcatcttcca gcagcggttt tagcaactcg agtgcacgct
    15001 cgtgagagat ttgatcgggc gcatcaagat aatcatgagc aaccggaata tatgccgcta
    15061 cgcctggctc gatagcaaaa gaaagcccga ccaggttagc agagatgtta tcaaggctgt
    15121 cggtttcggt atcaaatgca aataccggcg ctttttccag cttcgcaatc cacgctttca
    15181 gtgtttcttc atcaaggata gtgacgtagt tgtcataaga aatcaccgtt gccgtcactt
    15241 ctggcgcttc gtctgcaaca ctggtttcct gcggcttcgc ggctgttttt gcccctttgg
    15301 cctgtaacca tttgcccact tcgacatcag cagtccagcg tttgaactca tactttttga
    15361 acagccccaa caactcttcc gctgccggtt gctgcacttc cagttgttca caggtcagct
    15421 ccagttcaac atcggtttta atcgtcgcca actggtatga gagataagca acttctttgt
    15481 tttgctcgag cttcgctgcc attgttttcg cgccacggaa gctcaaccca gcaatttttt
    15541 ctggctcggc atacagcgta tccagcccgc caagaccttg cagcaatgcc tgcgcggttt
    15601 tttcaccgac gcccggtacg ccaggaatgt tatcagagga gtcacccatc agcgccagga
    15661 agtcgatgat caattctggc ggcacgccgt acttattcac cacctcttcc ggtccgagga
    15721 tggtattcgt catggtattg ataagcgtaa tatttggcgt caccagctgc gccatatctt
    15781 tatcgccagt gctaatcagc accggacgcc cggctttttc ggcttcgcgc gccagagtac
    15841 cgataacgtc gtccgcttct acgccagaaa ccgccagcag cggcagtccc atcgctttaa
    15901 ccatcgcgtg cagaggttcg atttgtgctc gcagatcgtc cggcattggc gggcgatgtg
    15961 atttgtaatg ttcaaacagt tcatcacgaa aggtttttcc cttggcgtca aagaccaccg
    16021 ctgcatgcgt cggtttatat tgcatgatca gactgcgcag catgttgagg acaccataca
    16081 tcgcaccggt cggctcgcct gcgctgttag tcagcggggg aaacgcgtga tatgcgcgat
    16141 aaagataaga tgaaccatct acaaggataa gtggattttg ggggatctga accataatgt
    16201 ccgtgcctgt ttatcagatt atgggtaaag gatgccacag acaggatgaa aacatgagtt
    16261 tttcaccgct tttcgtgaaa atcttttgta gatctgctgg atcgctcgca aatcgtcatg
    16321 tggataactt tgtgcataaa ttatttacat cgcttttaat ttattatcat gctttttaca
    16381 gcatgatgtt tgtctttgtt tttcatgcag ttaaaaataa atccatccct aataattgag
    16441 ataatgggat tttttctaat gtggatagat atgaattatt tttctcctta aggatcatcc
    16501 gttatttggg tcattttctg ctaaaatctg cgccttgcgc ttctttagtg cactgtctat
    16561 ggctaacctt ttgaatgaat tcattatgac gagaatactc gctgcgataa cccttttgct
    16621 gagtatcgta ttgaccattt tggtcactat tttttgttcc gtcccgatca tcattgccgg
    16681 gattgtaaaa ctattgctac ctgtgccagt catttggcga aaggtttcgc gtttttgtga
    16741 ttttatgatg tattgctggt gtgaaggtct ggcggtatta ctgcatctga acccacacct
    16801 gcaatgggaa gttcacgggc tggaagggtt aagtaagaag aactggtatc tgcttatttg
    16861 taaccaccgt agttgggcag atattgtcgt actgtgcgtg ctgtttcgta agcacattcc
    16921 gatgaataaa tatttcctca agcaacagct ggcctgggtg ccatttcttg gcctggcgtg
    16981 ctgggcgctg gatatgccgt ttatgaagcg ttattcccgc gcttatttat tacgtcatcc
    17041 ggagcgacgt ggtcaagatg ttgaaaccac tcggcgttct tgtgaaaagt ttcgcctgca
    17101 tcccaccacc attgtcaatt tcgttgaagg ctcccgtttc acgcaagaaa aacatcagca
    17161 aacccactcc acttttcaaa acttgttgcc accaaaggcg gcaggcattg cgatggcgct
    17221 aaatgtactg ggtaaacaat tcgataaact gcttaatgtt acgctgtgtt atccggacaa
    17281 taaccgtcag ccgttcttcg atatgttaag cggtaaactg acgcggattg tcgtacatgt
    17341 ggatttacag cctattgccg atgagttaca tggcgactac atcaatgaca aaagctttaa
    17401 gcgccatttt cagcaatggc tgaactcatt gtggcaggaa aaagaccggc ttctgacgtc
    17461 tctcatgtca tcacagcgtc agaataagta agttttactc gcctctgaat gagcagaggc
    17521 gagtgagtat tttaatgaac aaaacgtccg gcacgagaca taaattcttc ttcgctcatc
    17581 tcctgtccgt taaaaacaac tttacccggt gtataacgca gctgcagcga ggcggtattg
    17641 ttgtcaatcg tgattaactg aaacatctga cctaatgtca tcatcccgct gatttgttta
    17701 tcagcctgct tttgcgcttt ttccgcatcc attccttcag ataaattaag ctgttttgcg
    17761 gtttctgtga cgacattaag cggtaacttc acatcaaaat tgagcgattt gatatctttg
    17821 tttgtggatg atgaagattt ggctgggtcg gcaatactga tatccagatt ggcatttagt
    17881 tcgccgagtg cgttcttcca tcttaccggt tgtttaatgg tcggctcact tttttgtaat
    17941 aacggtaaat actctttgaa caatgcagca ttcacttctt gcagagcaac ttcatcgttt
    18001 gctaactcag ggtgtgcagc aagctgcttc tgcatcgcaa tgttatactg gatgataaat
    18061 tggcgcactg ctgagggatc aatagattca gcaatcaatg accactcacc actaccgagt
    18121 tgctgattcc cttttgttaa cttatcaagt tcgtaggtca gattgacatt gatataatct
    18181 ttgtctttaa cgcgatccag cctggttttt gcaacgaaag catcgatttg ggcaacgtct
    18241 tccccgtgat tgataatgtt aatctgatta aatttcgact cgctttcacc aaccggaaat
    18301 ttcttttctt ctaatcgcgc cagagaatca atggtaaaac tttttgccgt tactttgttc
    18361 attggtgata attgcagaac aatatcttcg actttgcctt caatatccag gttagacaat
    18421 gaactaccat caccattaaa agtaaactgg ccctcaccaa aagaaaattt tccatactct
    18481 gcaggggcaa catctaatgt cgtagagaat gagccaccaa acgcgaatcg caatgtcgct
    18541 tccacgggcg atttattttt cgcggcgata aatagcggtt gtgtcagttc attattcact
    18601 aagttcactt ttgccgctgc cagtgctggg atgacattac catgcattaa cattgtgatg
    18661 ggcaacggac cgtgctcaac atccacatca aatacaactt tctggcctgg cttgatattg
    18721 agatcgggtg caccgttatc gaatgtcatt tgcatttgaa aacgggaatt aaaaaagcct
    18781 ttgtcaaagt ttttataact taacgtcata tcgtaggcat gttcaccttt ctttttcgca
    18841 tcgttaaaat ctttaataaa cttttcgata ccaggctgaa tttgcgtacc tgtgtaccat
    18901 gtgccaccac cccagattac ggctaacgca acaataacac ctgtagctga cttacgtatc
    18961 atataaatat tccctgataa aacagcaaaa ctttgaatat ccacttatgc tgaaataatg
    19021 tccagcggca ggatgcatta tcagaatgta tttatattta ttaaataata aaaaaagccc
    19081 gtgaatattc acgggcttta tgtaatttac attgaattat ttttcctcgg acagatattt
    19141 caccgtatca gcatactgct gaacaaaaac atccatattg ctggtatcca taccctgcgg
    19201 attcagctga tatttaccgt taacaaacat cgccggaaca ccacgcaatt gcacatcagc
    19261 tgcggctttt tcctgctgag cgaccagaga tttcaccacg aagctgttcc acgccgcgtc
    19321 gtactcttca cctttaatac ctgcattgat aaatacatcg cggatatcag atgctgaacg
    19381 aatggtctgg gttttctgta cgccttcaaa cagcggaaca gtcactttgt cttccacgcc
    19441 cagcgccatc gccacagccc atgcctgagt cagatctttg cccaggtcac cacccatgaa
    19501 gttgacgtgg tatttagtca tcttcacgcc ttccggcagt tttttcttca cattatcaga
    19561 aatatgcaga acttcttcaa actgatagca gtgcgggcag aagaaagaga aaaactccag
    19621 cacttgcggc gcgccagcta ccggtttttc cagggtagtg tactgtttac catcttcata
    19681 ctgcgccgcc gatgcgctaa acgctaaaac taaaccagcc agcgccagcc aaatcttttt
    19741 catgatctac tctctccgat taatacatag gtgttaattg caaagggggt tcttgtagaa
    19801 cttttgcctg ttctataaaa gtcgccgtct gtcgtagcca gtaatcttcc ccggttaacc
    19861 acgggaaatt tttcgggaac gcgggatcag cccaatgccg cattagccag gcaagataat
    19921 aaaccaaacg catggcgcgt aaaggttcaa tcagtccgat ttcagcggtg tcgaactcgc
    19981 taaattcttc ataagcttca ataatagttt ccagttgcat ccgctgctgg gctttatcgc
    20041 cattgagcaa catccacaaa tcctgaatag ctggaccatt acgtgcatca tccagatcaa
    20101 caaacattgg accatcgcgc cagagaatat tcccggcgtg gcagtctcca tgtagccgca
    20161 gaacggtgaa atcttcccgc cagtgtgctg taacggcggc gatcagctca tctgtcgctt
    20221 tcagaaatgc cgctttcaac ccggaaggta tcagtgtagc gtcctcaaac agcttgcgag
    20281 gctcaatgag atattcattc agaccgatgg tcgggcgatg aataaaaagc tgtttgcgcc
    20341 ccgtctggtg catacgccct aaataacgcc caaccgcttc catctgatcg atattatcag
    20401 cttcgaactg gcgaccaccg acgcttggaa aaacagcgaa ataaaatccc tgatgattca
    20461 ataaagtctg accgttaaag gccacaggtg ctgcgaccgg aacttcatca tttaccagct
    20521 gcaacgcaaa ttgatgttct tcgaggattt gatcggctgt ccaacgttca gggcgataaa
    20581 atttgacgac aaaacgtcga cgctcttcgt cctgaaattg atagacacgg ttttcatagc
    20641 tgttaagcgg ggtaagaccg gaatccaccc ggatcccttg cttaaacaga gcgtccatga
    20701 tggtatccgg gtgtagtgtc tggaaagtaa aagcgctgtt gttcatccca tcatccggaa
    20761 aacattacga atgatgaagg atatcatttc acgatgattt tggtggcgcc gcttacaagc
    20821 ttttactctt taattacgcc gcgagcgcgt aacagcgccg ttttgaagtc ttcttcataa
    20881 tctttctgca aacccgggat caccgcatct ttcgcggaat cacgcatttt caggtgatag
    20941 atcagaatgt catccgtcaa atccgccagt tcgccgtcaa aacctgactc tttcgccagt
    21001 ttctgcaaaa attgcaacaa gttaaagtct ggctcttttt gccaggctgg ctggaggagt
    21061 tcaataactt cattcagacg tttacatttc atagtaatgc tccttactct tgagacagac
    21121 acgttagcag ggtcaatccc acaataaaag aggcgatatc ggtgaatcta atgacgacga
    21181 taacaggcgt tgtgctggca ggcggtaaag ccagacgaat gggcggcgta gataaaggat
    21241 tgctggactt aaatggcaaa ccgctgtggc aacatgtcgc tgacgcgctt aagacgcagc
    21301 tctctcacgt cgtggttaat gctaatcgtc atcaggaaat ctatcaggca ggcggtctga
    21361 aagtgattga agattcactg gcggattacc caggccctct ggcaggaatg ctttcagtaa
    21421 tgcagcagga agctggtgag tggtttttgt tttgtccgtg cgatacgcct tacattcccc
    21481 atgatttagc cgcccggctt aatcatcagc gcaaagatgc gcctgtcgtg tgggtccatg
    21541 acggtgaacg cgatcacccg actattgctc tggtaaaccg cgctattgag cctttattac
    21601 tggaatatct gcaagcagga gaacgccggg taatggcatt tatgcgtctg gctggcggtc
    21661 atgcggttga tttcagcgat cataaagatg catttgttaa cgtgaatacg ccagaggagc
    21721 ttgcccgatg gcaggaaaaa cgatgatacc gttactcgcc tttgccgcgt ggagtggtac
    21781 cggaaaaact acgcttttga aaaaactgat cccggcatta tgcgccagag ggatccgtcc
    21841 ggggctgatt aagcatacgc accatgatat ggatgttgat aagccaggca aagatagcta
    21901 tgagctgcgc aaggctggcg cggtacaaac catcgttgcc agccagcaac gatgggcctt
    21961 gatgacggaa acaccagacg aagaagagct ggatctacat tttctcgcaa gtcgaatgga
    22021 tacctcaaag ctggatttga ttctggtcga agggtttaag catgaagaga tcgcaaagat
    22081 tgtgctgttt cgcgatggag ccggacatcg acctgaagaa ttagtgatag acaggcatgt
    22141 tattgctgta gccagtgatg tgccgcttaa tcttgatgtc gcattactgg atattaatga
    22201 tgttgagggg ctggctgatt ttgtggtgga gtggatgcaa aagcagaacg gataagcaag
    22261 agtaggctgg atcagacgc
//
LOCUS       DADLTY010000059        22244 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.059, whole genome shotgun sequence.
ACCESSION   DADLTY010000059 DADLTY010000000
VERSION     DADLTY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.059"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            68..826
                     /locus_tag="JN334_001882"
     CDS             68..826
                     /locus_tag="JN334_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438040.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            872..2221
                     /locus_tag="JN334_001883"
     CDS             872..2221
                     /locus_tag="JN334_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAY7438041.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            2221..3045
                     /locus_tag="JN334_001884"
     CDS             2221..3045
                     /locus_tag="JN334_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438042.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            3057..3617
                     /locus_tag="JN334_001885"
     CDS             3057..3617
                     /locus_tag="JN334_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY7438043.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            3648..4718
                     /gene="lptF"
                     /locus_tag="JN334_001886"
     CDS             3648..4718
                     /gene="lptF"
                     /locus_tag="JN334_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY7438044.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            4715..5794
                     /gene="lptG"
                     /locus_tag="JN334_001887"
     CDS             4715..5794
                     /gene="lptG"
                     /locus_tag="JN334_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY7438045.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5830..7001)
                     /locus_tag="JN334_001888"
                     /pseudo
     CDS             complement(5830..7001)
                     /locus_tag="JN334_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(7001..7249)
                     /locus_tag="JN334_001889"
     CDS             complement(7001..7249)
                     /locus_tag="JN334_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7438046.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(7282..8178)
                     /locus_tag="JN334_001890"
     CDS             complement(7282..8178)
                     /locus_tag="JN334_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAY7438047.1"
                     /translation="MTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNLTWVRGS
                     LEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGFCQRFLF
                     ISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKPLFDWML
                     RGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDWQRVRQL
                     AAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADWSASNNR
                     ISEDINWFPGISLEHALRNGLF"
     gene            complement(8193..9923)
                     /locus_tag="JN334_001891"
     CDS             complement(8193..9923)
                     /locus_tag="JN334_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HAY7438048.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRTEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            10285..11426
                     /locus_tag="JN334_001892"
                     /pseudo
     CDS             10285..11426
                     /locus_tag="JN334_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(11550..12320)
                     /gene="glcC"
                     /locus_tag="JN334_001893"
     CDS             complement(11550..12320)
                     /gene="glcC"
                     /locus_tag="JN334_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HAY7438049.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            12571..14070
                     /gene="glcD"
                     /locus_tag="JN334_001894"
                     /pseudo
     CDS             12571..14070
                     /gene="glcD"
                     /locus_tag="JN334_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            14070..15122
                     /gene="glcE"
                     /locus_tag="JN334_001895"
     CDS             14070..15122
                     /gene="glcE"
                     /locus_tag="JN334_001895"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HAY7438050.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            15133..16352
                     /gene="glcF"
                     /locus_tag="JN334_001896"
                     /pseudo
     CDS             15133..16352
                     /gene="glcF"
                     /locus_tag="JN334_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            16357..16761
                     /locus_tag="JN334_001897"
     CDS             16357..16761
                     /locus_tag="JN334_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438051.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            16783..18954
                     /gene="glcB"
                     /locus_tag="JN334_001898"
     CDS             16783..18954
                     /gene="glcB"
                     /locus_tag="JN334_001898"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HAY7438052.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            19309..20895
                     /locus_tag="JN334_001899"
     CDS             19309..20895
                     /locus_tag="JN334_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease glcA"
                     /protein_id="HAY7438053.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
BASE COUNT         5383 a         5393 c         5927 g         5541 t
ORIGIN      
        1 gcgtcactac gcgcaattca cgccgcgctt tcctgatcac caaaatggat aggggatgat
       61 ttaacgaatg gatgcactac aaacacaaac tgttaatagt acaaccgcgc cgcagcccaa
      121 ctacattccg gggctgattg cggtggtcgg gtgtgatggc accggtaaat ccacgctgac
      181 caccgacctg gtgaaatcgc tgcaacaaca ctggcaaacc gagcgccgct atctggggct
      241 gctctccggc gaagacggcg acaaaatcaa acgattgccg ttggttggcg tctggctgga
      301 acggcgattg gcggccaaat cctcgaaaac ccaaagcatg aaaaccaaat ctccggcgct
      361 atgggcggcg gtgattatgt actgcttctc gctgcgaaga atggcgaatc tacgcaaggt
      421 tcagcgactg gcgcaaagtg gcgttctggt ggtcagcgat cgcttcccgc aggctgaaat
      481 ttcgggcttt tattatgacg gaccggggat cggcgtcgaa cgtgcgaccg ggaaaatcag
      541 catgtttctg gcgcagcgcg aacggagttt ataccaaaaa atggcgcaat accgtcctga
      601 attaatcatt cgtttgggga ttgatattga tactgccatc tcccgcaagc ctgaccatga
      661 gtatgccgag ctgcaggaca aaatcggcgt catgtcgacg attggctata acggcacaaa
      721 aattcttgag attgattcca gagcgcccta cagcgaagtg ctggaacagg tacagaaggc
      781 ggtttctctg gttgccatcg tttctgaccg ccgaagttta acttaaggct ggcgggatat
      841 tttttactat caacgataag gtcaggctga attggcgggt tttaacatca aacattggtt
      901 tgcagatggc gcgtttcgca ccattattcg caatagcgcc tggttaggct ccagtaatgt
      961 cgagagcgcc ttgctggggc tgttggcgct ctcgtgtgcc ggtaaaggga tgacgcccgc
     1021 catgtttggt gtactggtga ttgtgcaatc gtacgccaag tcgatcagcg attttattaa
     1081 gtttcagaca tggcagctgg tggttcagta cggaacgcca gccttgacca acaataatcc
     1141 gcagcaattt cgcaatgtcg tctcattttc cttctcgctg gatatcgtca gcggcgcggt
     1201 ggcgattgtc ggcggcattg ccttactgcc attcctttcc cattcattag gtctggatga
     1261 ccaaagcttt tggctggcgg cgctgtattg cacgctcatt ccttcaatgg cttcctccac
     1321 gccgaccggc attctgcgtg cggtagatcg ctttgatttg attgctgtac agcaggcgac
     1381 gaaacctttt ctgcgcgcag cggggagcgt cgcagcctgg tattttgact ttggttttgt
     1441 gggttttgtt attgcctggt acgtgtcgaa tctggttggc ggcaccatgt actggtggtt
     1501 tgccgcgcgc gaattacgcc gtagaaatat ccataacgcc ttcaaattga atctgtttga
     1561 gtctgcccga cacattaaag gcgcgtggag ttttgtctgg tcaaccaaca ttgcccactc
     1621 catctggtcg gcgcgtaact cgtgcagcac agtgttggtg gggatcgtgt taggacccgc
     1681 tgccgccggg ttatttaaaa tcgccatgac attcttcgac gccgccggaa cgccagcggg
     1741 tttgctgggt aaaagttttt acccagaggt gatgcgttta gatccgcgca ccaccagacc
     1801 gtggttgtta ggtgtgaagt ccggtttact ggcgggcgga atcggtattc tggtggcgct
     1861 cgcggtgttg atcgtcggca agccgctcat ttcgctggtg tttggcgtta agtatctcga
     1921 agcgtatgac ctgattcagg tgatgctggg cgcaattgtg atttcgatgc tgggcttccc
     1981 gcaggagtca ttgctattga tggcgggaaa acagcgcgct tttctcgtgg cgcaaaccat
     2041 cgcctcaatc ggctacatcg tattgctgtt catgttctgt catctgtttg gtgtgctggg
     2101 agcagcgttt gcttacttcg gtgggcaatg tctggacgtg gcgctctcgt taattccaac
     2161 cttaaaggca ttttttcagc gccattcctt gctttataac gcagccggag agaaatcctg
     2221 atgatgagaa aatatttccc gcttgaggcg agcgagcgtc tatttgttgc gattgaagaa
     2281 gatgatgtcg ttgatgcaca ggttagtttg ccgccgacta ttgcactaag ttgtaccacc
     2341 gaaatcattc atgataatta cgcgttatgc ctgcaattct ggctcaatgg cgttgatcgt
     2401 caggaattac tgcgtttagt tcgcaaacaa gccaaaggcg atgagctgac tgctgatgag
     2461 cgcaaacaat ttaaatacat gcgagcgcgc tacaagcacc tgcgttttgc gcaacggctg
     2521 tatttaaaga agcatcaggc cggattttta ttcggcaaaa cgaccgtttt tctggggcgt
     2581 tttcaggacg gctttcgtaa cggtaagaaa aatattgtgt cgtattacgg caacctgtta
     2641 cggatttatt taagctctcc ggtctggtcg ttggtgaact attcattacg ccatagccag
     2701 ttagaaagcg taagcagttt tattgcctat cggcaaaagc aaatgcatac cctgaaagag
     2761 attattgcca aaccgcgact aaccggcagg gaatttcatg atgtgcgaaa aatcatcagc
     2821 cagcaggttt cttattacga caccttaaga tcgcttgatc ctgaaaataa agaggcgtta
     2881 cagatttccc gtttcctcgc cgccattaat ggcctgatgg gagataaaca cgacgacatg
     2941 gtggcggatg atatggaaaa ccgccagtcg tattatgcac cgttggcgct ggatagcgat
     3001 atttgtcagc gcttagagct gctgatttcg cgttttccgc tatagccgga gcaatgatgt
     3061 catctcgttt gattattgcg ttgattatca tgctcttagc cccgggtgtg caggcacaca
     3121 atttcgtcac tggcaaaacg gttacccctg tttatatcca ggaaggcggc gagctgcttc
     3181 tgaatagcga cgacgaaatt cactaccaaa agtggaacag cacacaatta gcgggaaaag
     3241 tccgcattat tcagtatatc gccgggcgta agtcggcgaa gaaaaagaac tcactgttaa
     3301 ttaaagcggt tgaagcggca aactttccgc aggatcgttt tcagcccacc accattgtga
     3361 acaccgatga tgccatcttt ggtactggct attttgttgt tggcaaaatc gaaaaaaata
     3421 aacgccgcta tccgtgggcg caattcgtta ttgacggcaa tggacaggga cgcgttgcct
     3481 ggcgcttgcc ggagcagagt tcaaccattc tggtgctgaa taaagccggg caaattcagt
     3541 gggcaaagga cggcagcctg acgccggaag aggtagatca tgtcattgcg ttggcgcaga
     3601 aactgatcaa tgaataaaaa actcaaagga ttgagggcgt tagtgagatg aagctggttg
     3661 aacactacat catgcgtggt acgcgccgtc tggtgctgat tatcgtcggt tttctgatct
     3721 ttattttcgc cagctactcc gcacagcgtt atctgaccga agcggcaaat gggacattag
     3781 ctcttgatgt tgtgctggat atcgttttct acaaagtact gattgcactg gagatgttgt
     3841 tacctgttgg tctgtatgtg tcagttggcg tgacgctagg gcagatgtat accgactcgg
     3901 aaattaccgc tatctctgcg gcggggggca gtccgggacg cttgtacaaa gccgttcttt
     3961 atctggcgat accgctaagt atttttgtca cccttctgtc gatgtatggt cgtccgtggg
     4021 cttatgcgca gatttatcaa ctggagcagc agtcgcagtc ggagctggat gttcgccagt
     4081 tgcgggcaaa gaaatttaac actaacgata acggacgaat gatcctttcg cagacggttg
     4141 atcaggataa taatcgcctg actgacgcgc tgatttatac ttctactgcc aatcgaaccc
     4201 gtattttccg cgccggttcg gttgatgtgg ttgacccatc acccgagaaa ccgaccgtta
     4261 tgttgcacaa cggcactgcc tatcttctcg atcatcaggg gcgtgacgac aacgaacaga
     4321 tctaccgtaa tctgcaatta catctgaatc cgctggatca aagcccgaac gtcaaacgca
     4381 aagcgaaatc ggtcacggag ctggcgcgct ccgcctttcc tgccgatcat gccgaactgc
     4441 aatggcgaca aagccgtggc ctgaccgcat tgttgatggc gctgctggcc atttcattaa
     4501 gtcgggtaaa accgcggcaa gggcgatttt caacgttatt gccactgacg ttgctgttta
     4561 ttgccatttt ttatggcggc gacgtctgcc gtacactggt ggctaacggc gcgattcccc
     4621 tcattcctgg tttgtggtta gtgcccggac tcatgctaat gggcctgctg atactggtcg
     4681 cacgtgactt ctctttgctg cagaaatttt cccgatgaat gttttcagtc gctatttaat
     4741 ccgtcatctc tttctcggtt ttgccgccgc tgcagggcta ttgctgccgc tttttaccac
     4801 cttcaacctg attaacgaac tggatggtgt cagcccgggc ggttatcgct ggactcaggc
     4861 ggtgctggtg gtgctaatga ccttaccgcg cacactggtc gaactttcgc cgtttatcgc
     4921 gttattgggt ggtattgttg gactggggca gttatcgaaa aacagtgagc ttaccgccat
     4981 tcgcagcacg gggttttcta tcttccgtat tgcactggtg gcgctggttg ccgggatatt
     5041 gtggactgtt tcgttaggcg cgatagatga gtgggtggcg tcgccattgc agcaacaggc
     5101 gttgcaaatc aaatcgaccg ccaccgcgtt gggggaggac gatgacatta ccggcaatat
     5161 gctgtgggcc aggcgtggta atgaatttgt gacggtgaaa tcgctgaacg agcagggcca
     5221 gccggtgggc gtggagatat ttcattatcg cgacgatctc tcgcttgaat cctacattta
     5281 tgcacgcagt gccaccattg aagacgacaa aacgtgggtc ctgcatggtg tgaatcataa
     5341 aaaatggctg aatggcaaag aaacgctgga aacattagat aatcttgcct ggcaatcggc
     5401 cttcaccagt atgaatcttg aagagttatc aatgccgggg aatacttttt ctgtccgtca
     5461 gcttaatcat tacatccatt atttgcagga aaccgggcaa cccagcagcg aataccacct
     5521 tgcactgtgg gaaaaactgg ggcaacccat cctgaccctg gcgatgattt tgctggctgt
     5581 gccgtttacc tttagtgccc cgcgttcgcc tgggatgggt agccgtctcg ctgtaggcgt
     5641 catcgtgggc ttactcacct ggatcagcta tcaaatcatg gttaatctgg gattgctatt
     5701 tgcgttaagc gcacctgtta ccgcgctcgg tttaccggta gcgtttgtgt tggtggcgtt
     5761 gagcctggtg tattggtatg acagacaaca ttaaacccgc cgtggcgcaa acaccacggc
     5821 gggctcacat tactgcttca gagtagcaaa tgcctgaatg atggcatcga tttgctcacg
     5881 actgtgggca gcatttacgc tacagcgcag cagcgtgatg cctgcaggcg cggcaggcgg
     5941 caggataaga ttcacataca cgcccggcga gataagctcg cgccagatgc gtaaaccttc
     6001 ctctttcgaa ccgattatta ctggcactac cgggctaata tgtgaactta actcataacc
     6061 cagtttttgc agaccgtcat acagatggtt ggcgttatcc atcagctttt gccgcagctc
     6121 tggttgtgtg gcaatagttc ttaacgacga gcgcacggtc gcaatgcagg acggagaagg
     6181 agaggcggta aaaatatacg ggcggctgcc gtagcgcagc acttccatcg cttccgaaac
     6241 gacagcaaaa ccgccaatcg aggccagact tttactgaaa gtaccaacga taatatccac
     6301 atcatcttca acgccgacag cttcagccag tccgcgcccc gttgcgccta acacgccaaa
     6361 cgaatgcgct tcatcgacaa tcaaataacc accgagacga cgtttgatat caacaatttc
     6421 cgccagcggc gcaacgtcac cgagcatgct gtagatgccc tcaacgatga taatcgcctc
     6481 tttcgcccgt tcaccgaggc gaaccatgcg ccgttccaga tctttagcat cgttatggcg
     6541 gaaacgaata atttccgcgc caccgagaga acagacgtca taaatactgg cgtggctgtc
     6601 ggcatccagt aacaccacgg cgttatggtc ggcaagtgcg ctgatgacgc ctaaattagc
     6661 ggtataaccg gttgaaaaac gatagcggta gggcgattga aaaactcggc aatctctttc
     6721 tctaacgcca ggtgcggcgc ataactgccg tttgccatac gagaaccggt ggtgccggtg
     6781 ccttgggccg ctaacgcggc ctgaccttcg ctgatggcat catgattaaa ggttaaaccg
     6841 agataattat tggtcccggc cagaatcact tttttattgc cgatccggcc ttcggtggcg
     6901 gaatacactt catcaataca ggtgccaaac ggtgttaacc cgttgtcact aaattgcaga
     6961 cgctcgccag caaggcgcgc atatttatcg tatagcccca ttatttattc tccagccatg
     7021 gaagtagagt ttccattaat tgcgcagggg ttttgacatc cagcagaata ttaataggaa
     7081 tggaaatatc gaatctgtct tccagcatca ttaagagatc catcactttg atcgactcca
     7141 ggccaagatc gttgacgaga tcgctgtcgg gcttaatttc cacaccgttt tcgaccaaat
     7201 cctgtaaaca ggaaaggatg taatccatta ctatttcacg atttaccata gaaacgttaa
     7261 cacaccttta aaacaagaca atcaaaatag cccgttgcgt aatgcgtgtt ccaggctaat
     7321 cccgggaaac caattgatat cttcagaaat acggttatta cttgccgacc agtcggcgtg
     7381 ggttaattca cgaattttcg agcgggtcag cataggttct ttacctgcca ggcgactcaa
     7441 cgcggtactg atatccgcaa ggcctgtgag taccggcaga ggaatgtcaa ccattcgcac
     7501 ggaaccacaa cgggctttgg cggcaagctg tcgtacgcgt tgccagtcat agccaccggc
     7561 gacgccatcg cataattcat aggtttgcgt ctgtacagtt tcggcgctta accactgacc
     7621 cactgcttgc gcaaaatcgg tgacgtgaag aaaagagagt tgtgtatctg gcgcaccaag
     7681 tcgtggcagc aggccgcgta gcatccagtc aaacagcggt tttaactctt tatcgccggg
     7741 accatacacg gctgtcgggc gaaaaacgcc cagcgtaatt tcgtccgcca ttgctgccag
     7801 tcgttgctcg gcgacgtatt tggattttgc ataccaggag agctcgggat ggcgcgccgc
     7861 cagcgaagag ataaacagaa aacgttggca aaagccgctc tcttttgctg cttgcatcag
     7921 gcgcaggctg ccgtcaacgt tacagcgggt gaaaatctct tctttgtgcc cgcgcacttg
     7981 cccggcgcaa tggaccacta cgctggctcc ggcaaccagc tcgctaagtg aatgagtatc
     8041 ttccagcgaa ccgcgcaccc aggtgagatt atcattgacg tgagcgcggg cagtacgcgt
     8101 caatgcgcga acgtgaaagc cgcgggcgag caggttatcg ataatatatt taccgataaa
     8161 cccggtagcg cccgtcaccg cgacagtttg atttatgcca gggattcctg cacattaaga
     8221 ctggcagcat aagccttctg ataacgtttt ttcgcttccg cacgggcagg cttgccggag
     8281 gacgttcggg gaatactgtg gggcggcaac agatcgatag ccgcggtcac gccaaattcg
     8341 ctttggatcc gtgccgccag cgcgtggata agctgcccgc gacgttcttc gtcgctgatc
     8401 cgacactgga tctgcaaaat gattttttcc tgggcggtaa caaaagcaat cgcatcgcca
     8461 gaatgaattt ccggttcctg ttccgctata tattcaatat cctgcggcca gatattacgg
     8521 ccacgaataa taatcagatc tttaatgcgt ccggtgacat acagataacc gtccagcaga
     8581 taaccgaggt cgccggtgtc taaccagccc gtcgcggcaa tctcgtcttg cgaaacctgg
     8641 tcgccaaagt aaccgctcat cagactggga ccggagatgc aaatatggcc taccacacgt
     8701 tccgcgaccg gcatacctgc ttcattgcgg atttcaatac catgttccgg caacgctttg
     8761 ccgcagttga cgaaagtcga tacggcgcgt gtctctgcac ccggcgcgac ggctttaccc
     8821 tgatattcga ggatgtcgcg atccacttcg ttaaccacaa ccccggaggc ttcatcagag
     8881 aagctgacag ccagcgcatt ttctgccagt ccgtagcacg gcatgaaagt tttattgtca
     8941 aagttaacct gacggaaaca ttcagcgaat tgatggagtt gttctgcgga gatcggctca
     9001 gcaccaatac cagcgacgcg ccagcaggaa agatccagtt cagcgagatc tttttcattc
     9061 acgcggcgtt ggcacaattc atagccaaac ggcggcgcaa cggaaacggt gccgcgattt
     9121 ttactgatca atttaagcca ttgcaaagga cgcatggcaa aatcctgagt gcgcaaataa
     9181 tctactgaaa gttgcgtggc gacggggggc aggagaaagc cgaccagtcc catatcatgg
     9241 tagaaaggct gccaggagac gcagcggtcg ctagggcgta atttaatgcc atcgtggctt
     9301 atagcacgta gattagccat cacttcgcga tgggtgataa tgacgccacg gggaaaacgg
     9361 gtgctgccgg aggtgtactg gaggtaggcg atatcgttcg gaactggacg ctggagcgca
     9421 acatcggctt ccggtaatgc cttaaaccaa gcgtggctta aaacatgtaa ttcggggttg
     9481 ttatgcgtcg cggcattgac cagtggcaac cactcatcac cagtgataat ggctgcgggc
     9541 tggcaacttg ccagtaaacc ctggagtttg gcgctccagg aatcccgctg accgacgccc
     9601 attggaatcg ccaacgggac ggcgactaag ccagcatact gacaggcaaa aaaagcctct
     9661 acaaacccgc tactcgtttc ggcaatcagc gccacgcgtt cgcctttttt caggttcagc
     9721 gataacaacc gcttcgcacc agcttcggta cgcgttttta atgtctgata ttccagttga
     9781 tcttcaagtt ggcaacgtct gtcataaaaa ttcattccgg ctctactcag agcggcgtag
     9841 tccaaagcat caaccagcgt tggaaaatcg gcatagcgca taggtaggga atgcgtaaag
     9901 attttattag acatatacac cacgaaaaaa ttggatatca aaaataaaat gacaataatg
     9961 cggtggatta tataggcata aaagataaat gagatagtat tttcatcggt gtgaaaatga
    10021 acattgattt aaataatgag tttaagtttt ttgtttttat tatgaatgat atttaattta
    10081 tgaatatatt aagaagaagc cttgtccggt cgccttaaat tgacaaaagg cattgaggcg
    10141 tttctgtgta gcctctgacc ttatcataat cattaattaa tactatttat tcgtcgtttg
    10201 tattcattga gttgattatt aagatgccgc tcgttgctgt tgcaacctgt ttttcaccat
    10261 taattatttg ttatccacag agatatggaa tgcgatctgc tgatgattaa aattgaaaaa
    10321 gtaattaata aaaatgacct taaggcattt atcgcttttc cgtcatcact ttatcccgac
    10381 gatccaaact ggataccccc tttattcatt gaacgcaacg agcatttgtc tgcgaaaaat
    10441 ccggggacgg accatatcat ctggcaggcg tgggtggcaa aaaaagcggg gcagatagtt
    10501 gggcgtatta ccgcgcaaat agataccttg caccgtgagc gttacggtga agataccggt
    10561 catttcggca tgattgacgc cattgatgat ccgcaggatt ttgccgcgtt gtttggcgca
    10621 gcggaagcgt ggttgaagtc acaaggtgca agtaagatca gcgggccttt cagcctgaat
    10681 atcaatcagg aaagcggatt actgattgaa ggttttgaca caccaccctg tgcgatgatg
    10741 ccacacggca aaccgtggta tgccgcgcat attgaacaac tgggttatca caaaggcatt
    10801 gatttactgg cgtggtggat gcagcgaacc gatctcactt tctctccggc gctaaaaaaa
    10861 ctgatggatc aggtgcgcaa aaaggtgacc attcgctgca tcaatcgtca gcggtttgcc
    10921 gaagagatgc agatcctgcg tgagattttc aactctggct ggcagcacaa ctggggattt
    10981 gtgccgttta ccgaacatga attcgcgacc atgggcgacc aacttaaata tctggtgccg
    11041 gacgatatga tctacatcgc tgagattgat tctgcaccct gcgcgtttat tgtggcttgc
    11101 ctaacatcaa cgaggcgatt gccgatctga acggatcgct ctttcccttc ggctgggcaa
    11161 aattgctgtg gcgtttgaaa gtcagcggtg tgcgaactgc gcgagtaccg ctgatgggcg
    11221 tgcgcgacga gtatcagttc agccgcatcg gcccggtgat tgcgctgtta ttgattgaag
    11281 ccttacgcga tccgtttgcc cgccggaaga tcgatgcgct ggagatgtca tggatcctcg
    11341 aaaccaatac aggcatgaat aacatgctgg aacgcattgg ggcggagccg tacaagcgtt
    11401 accgattgta tgaaaagcaa atttaatacc agatcctgtt tagcgcaggg gcggtcattc
    11461 gggagagggt atagatgaac agatttaaca ccagatcctg ttaagtgcag gggcggtccc
    11521 ctcgccttgg ggatagggta aaactctgct cacattccca ccttgttcat ctccagcggc
    11581 accgccgagc gaatcaaatg gtggccttcc agctcgatat cgtggagatt ctttttcacg
    11641 gtccgtacat gatcgcgcgc tgcgcgctga gcgacgtgcg gcagccgctg caacaccgcg
    11701 ttgtagatcc gcgcatgctg gcgatcgatc tgcttttttt gctgtggtcg atggtagaga
    11761 ttatttacgc tggcaaacac tgaattaaac atcagatcgg tcaatgattg cagcgtaaac
    11821 accagcacct gattgtgaga agcctgacaa atggcgagat ggaaagcgtg atccaactgc
    11881 gcatgttcga tcagcgaaat ctctttgtgg ttctcactgg cggcgagcat tttttcataa
    11941 cagcgggtta tcacaacaaa atcagcctgc gttcccagcg ttgccgccag ccttgccgat
    12001 tcgccctcca gtaatgcgcg aacgtcgagc agatcgtaca gcgttcgcgg ctgcgtactg
    12061 aacagatgga tcagcgggct ggtgtcctgc tcccgattaa gccgtgcgac atgagaatcg
    12121 cgaccctgcg ccgtttcaat aatcccgcgc ccgcgcagca cggtcagccc ttcacgcagt
    12181 gcggagcgtg agaagccgag cttttcacac agtcgacgtt ccgagggaag cggctgaccg
    12241 accttcagta cgccgtcgat aattaaccgt tcgatactct ctgcaaccac ttcgcaaata
    12301 gggcgacgtt catctttcat tcccggacct cgtgcacagg taggaccaat ttttctgttc
    12361 ttaactcgca aaacacgcac atcacgcaag tgattatgtt acatcaattt aacattaagt
    12421 taaccaagac aaggtcacag agctggaaaa aaaatggtct gaccggtagc taaagagata
    12481 gacgaaaacg aaaagcccgc ttaataactg ttcacagaag cagcgcgcaa aaatcagctg
    12541 ccacacaaca caacaaagcg aagcctactc atgagcatct tgtacgaaga gcgtcttgat
    12601 ggcgctttac ccgatgtcga ccgcacatcg gtactgatgg cactgcgtga gcatgtccct
    12661 ggacttgaga tcctgcatac cgatgaggag atcattcctt acgagtgtga cgggttgagc
    12721 gcgtatcgca cgcgtccatt actggttgtt ctgcctaagc aaatggaaca ggtgacagcg
    12781 attctggctg tctgccatcg cctgcgtgta ccggtggtga cccgtggtgc aggcaccggg
    12841 ctttctggtg gcgcgctgcc gctggaaaaa ggtgtgttgt tggtgatggc gcgctttaaa
    12901 gagagcctcg acattaaccc cgttggtcgc cgcgcgcgcg tgcagccggg cgtatgtaac
    12961 ctggcgatct cctaggccgt tgcgccgcat aatctctatt acgcgccgga tccttcctca
    13021 caaatcgcct gttcaattgg cggcaatgtg gcggaaaatg ccggtggcgt ccactgcctg
    13081 aaatatggtc tgaccgtaca taacctgctg aaaattgaag tgcaaacgct ggacggcgag
    13141 gcgctgacgc tgggatcgga cgcgctggat tcacctggtt ttgacctgct ggcgctgttc
    13201 accggatcgg aaggtatgct cggcgtgacc accgaagtga cggtaaaact gctgccgaag
    13261 ccgcccgtgg cgcgggttct gttagccagc tttgactcgg tagaaaaagc cggacttgcg
    13321 gttggtgaca tcatcgccaa tggcattatc cccggcgggc tggagatgat ggataacctg
    13381 tcgatccgcg cggcggaaga ttttattcat gccggttatc ccgtcgacgc cgaagcgatt
    13441 ttgttatgcg aactggacgg cgtggagtct gacgtacagg aagactgcga gcgggttaac
    13501 tacatcttgt tgaaagcggg cgcgactgac gtccgtttgg cacaggacga agcagagcgc
    13561 gtacgtttct gggccggtcg caaaaatgcg ttcccggcgg taggacgtat ctccccggat
    13621 tactactgca tggatggcac catcccgcgt cgcgccctgc ctggcgtact ggaaggcatt
    13681 gcccgtttat cgcagcaata tgatttacgt gttgccaacg tctttcatgc cggagatggc
    13741 aacatgcacc cgttaatcct tttcgatgcc aacgaacctg gtgaatttgc ccgcgcggaa
    13801 gagctgggcg ggaagatcct cgaactctgc gttgaagttg gcggcagcat cagtggggaa
    13861 catggcatcg ggcgagaaaa aatcaatcaa atgtgcgccc agttcaacag cgatgaaatc
    13921 acgaccttcc atgcggtcaa ggcggcgttt gaccccgatg gtttgctgaa ccctgggaaa
    13981 aacattccca cgctacaccg ctgtgctgaa tttggtgcca tgcatgtgca tcacggtcat
    14041 ttaccttttc ctgaactgga gcgcttttga tgctacgcga gtgtgattac agtcaggcgc
    14101 tgctggagca ggtgaatcag gcaattagcg ataaaacgcc gctggtgatt cagggcagca
    14161 atagcaaagc ctttttaggt cgcccagtca ccgggcaaac gctggatgtg cgttgtcatc
    14221 gcggcattgt taattacgac ccgaccgagc tggtgataac cgctcgtgcc ggaacgccgc
    14281 tggtggcgat tgaagcggcg ctggagagcg ccgggcaaat gcttccctgc gagccgccgc
    14341 attatggtga agaagccacc tggggcggga tggtcgcctg cgggctggcg gggccgcgtc
    14401 gcccgtggag cggttcggtc cgcgattttg tcctcggcac gcgcatcatt accggcactg
    14461 gaaaacatct gcgttttggt ggcgaagtga tgaaaaacgt tgccggatac gatctctcac
    14521 ggttaatggc cggaagctac ggttgtcttg gcgtgctcac tgaaatctca atgaaagtgt
    14581 taccgcgacc gcgtgcctct ctgagcctgc gtcgggaaat cagcctgcaa gaagccatga
    14641 gtgaaatcgc cgagtggcaa ctccagccat tacccattag tggcttatgt tacttcgaca
    14701 atgcgttgtg gatccgcctt gagggcggcg aaggatcggt aaaagcagcg cgtgaactgc
    14761 tgggtggcga agaggttgtg ggtcagttct ggcagcaatt gcgtgaacaa caactgccgt
    14821 tcttctcgtt accaggtacc ttatggcgca tttcattacc cagcgatgcg ccgatgatgg
    14881 atttacccgg cgagcaactg atcgactggg gcggggcgtt acgctggctg aaatcgacag
    14941 ccgatgacaa tcaaatccat cgcatcgccc gcaacgctgg cggtcatgcg acccgcttta
    15001 gtgccggaga tggtggcttt gccccgctat cggctccttt attccgctat caccagcagc
    15061 ttaaacagca gctcgaccct tgcggcgtgt ttaaccccgg tcgcatgtac gcggaacttt
    15121 gaggagcagg ctatgcaaac ccgattaacc gaagagatgc ggcagaacgc gcgctggaag
    15181 ccgacagcat cctgtgcgcc tgtgttcact gcggattttg taccgcaacc tgcccaacct
    15241 atcagcttct gggcgatgaa ctggacgggc cgcgcgggcg catctatctg attaaacagg
    15301 tgctggaagg caacgaagtc acgcttaaaa cacaggagca tctcgatcgc tgcctcactt
    15361 gccgcaattg tgaaaccacc tgtccttctg gtgtgcgcta tcacaatttg ctggatatcg
    15421 ggcgtgatat tgtcgagcag aaagtgaaac gcccactgcc ggagcgaatg ctgcgcgaag
    15481 gattgcgcca ggtagtgccg cgtccggcgg tcttccgtgc gctgacgcag gtagggctgg
    15541 tgctgcgacc gtttttaccg gaacaggtca gagcaaaact gcctgctgaa acggtgaaag
    15601 ctaaaccgcg tccgccgctg cgccataagc gtcgggtttt aatgctggaa ggctgcgccc
    15661 agcctacgct ttcgcccaac accaacgcgg caactgcgcg agtgctggat cgtctgggga
    15721 tcagcgtcat gtcagctaac gaagcaggct gttgtggcgc ggtggactat catcttaatg
    15781 cgcaggagaa agggttggca cgggcgcgca ataatattga tgcctggtgg cccgcgattg
    15841 aagcaggtgc cgaggcaatt ttgcaaaccg ccagcggttg cggcgcgttc gtcaaagagt
    15901 atgggcagat gctgaaaaac gatgcgttat atgccgataa agcgcgtcag gtcagtgaac
    15961 tggcggtcga tttagtcgaa cttctgcgcg aggaaccgct ggaaaaactg gcaattcgcg
    16021 gcgataaaaa actggccttc cactgcccgt gtaccctaca acatgcgcaa aagctgaacg
    16081 gcgaagtgga aaaagtgtta cttcgtcttg gatttacctt aacggacgtt cccgacagcc
    16141 atctgtgctg cggttcagcg ggaacatatg cgttaacgca tcccgatctg gcacgccagc
    16201 tgcgggataa caaaatgaat gcgctggaaa gcggcaaacc ggaaatgatc gtcaccgcca
    16261 acattggttg ccagacgcat ctggcgagcg ccggtcgtac ctctgtgcgt cactggattg
    16321 aaattgtaga acaagccctt gaaaaggaat aaaaaaatga aaactaaagt cattcttagc
    16381 cagcaaatgg cgagtgcaat tattgccgca ggtcaggaag aggcgcagaa aaataactgg
    16441 tctgtttcca ttgctgttgc cgatgacggc ggtcatctgc tggcgttaag tcgcatggac
    16501 gattgcgcgc cgattgcggc ttacatctcc caggagaaag cgcgtaccgc cgcgctgggg
    16561 cgtcgtgaaa ctaagggcta tgaagagatg gtgaacaacg gacgtaccgc gttcgtgact
    16621 gcgccgttac tgacgtcgct ggaaggcggc gtaccggttg ttgttgatgg gcaaattatt
    16681 ggtgccgtgg gcgtttctgg tttaaccgga gcacaggatg cgcaggtcgc gaaagtggca
    16741 gcagcggtgt tggcgaaata agcgaaaacg aggagagaaa caatgagtca aaccataacc
    16801 cagggccgtt tacgcattga cgccaatttt aaacgttttg tggatggaga agttttgccg
    16861 ggcgtggaac tggacgctgc tgcattctgg cacaatgttg atgagatcgt tcacgatctg
    16921 gcaccagaaa atcgtcagtt gctggcagaa cgcgatcgca ttcaggcggc gcttgatgag
    16981 tggcatcgca gcaatccggg gccggtaaaa gataaagcgg cctataaatc tttccttcgt
    17041 gaactgggct acctggtgcc gcaaccggag cgcgtgacgg tggaaaccac gggcattgac
    17101 agcgaaatca ccagtcaggc ggggccacag ctggtggttc cggcaatgaa cgcccgctac
    17161 gcgctgaacg cggcgaacgc tcgctggggc tcactgtacg atgcgttata cggcagcgac
    17221 atcatcccgc aggaaggggc gatggtcagt ggctacgatc cgcagcgcgg tgagcaggtt
    17281 atcgcctggg ttcgacgttt cctcgatgaa tcactaccgc tggaaaacgg cagctatcag
    17341 gatgtggtgg cgtttaaggt ggtcgataaa caattacgca tccagttgaa aaatggtaaa
    17401 gaaaccacgt tacgtactcc agcacagttt gtcggttacc gtggcgatgc cgctgcgccg
    17461 acctgcattt tgctgaaaaa taacggcctg catattgaac tgcaaattga tgccaacggg
    17521 cggattggca aagacgatcc ggcgcacatc aacgatgtta tcgtcgaagc tgctatcagt
    17581 accattctcg actgcgaaga ttcggtcgcg gcggttgatg cggaagataa aatcctgctg
    17641 taccgcaatc tgctgggcct gatgcagggg actctgcaag agaaaatgga gaaaaacggt
    17701 cggcaaatcg tacgtaaact gaatgacgat cgtcagtaca ccgccgccga tggctctgaa
    17761 atttctctgc acggacgctc gttgctgttt atccgcaacg tgggtcattt gatgaccatt
    17821 cctgtgattt gggacagcga aggcaatgaa atcccggaag gtattcttga tggcgtcatg
    17881 actggcgcga ttgccctcta tgatttaaaa gtgcagaaaa actcgcgcac tggcagcgtc
    17941 tatattgtga aaccgaaaat gcacggtccg caggaagtgg cgttcgccaa caaactgttt
    18001 acccgcattg agacaatgct cggtatggca ccgaataccc tgaaaatggg cattatggat
    18061 gaagaacgcc ggacctcgct gaacttgcgt agctgtatcg ctcaggcgcg caaccgcgtg
    18121 gcgttcatca ataccggttt cctcgaccgt accggcgatg aaatgcattc ggtgatggaa
    18181 gctggcccga tgctgcgtaa aaaccagatg aaatcgacgc cgtggatcaa agcctacgaa
    18241 cgtaataacg tgctttccgg tctgttctgt gggctgcgcg gtaaagcgca aatcggtaaa
    18301 ggcatgtggg caatgccgga cctgatggca gacatgtaca gccagaaggg cgaccaactg
    18361 cgtgccgggg caaacacagc ctgggttccg tcaccaaccg ctgctacgct ccatgcgctg
    18421 cactaccacc ataccaacgt acagagcgtg caagccaaca ttgcccagac cgagttcagt
    18481 gctgaatttg aaccgctgct ggacgatctg ctgactattc cggtggctga aaacgctaac
    18541 tggtcggcgc aagagatcca acaagagctg gataacaacg tgcaggggat tctggggtac
    18601 gtggtgcgtt gggtggagca ggggattggt tgttcaaaag tgccggatat tcacaacgtg
    18661 gcgctgatgg aagaccgcgc aacgctgcgt atctccagcc agcatatcgc caactggtta
    18721 cgtcacggta ttctgaccaa agaacaggtg caggcgtcgc tggagaatat ggcgaaagtg
    18781 gttgatcagc aaaatgctgg cgatccggct tatcgtccga tggcggggaa tttcgctaac
    18841 tcgtgtgctt ttaaagctgc cagcgattta atcttcctcg gcgtgaaaca gccaaacggt
    18901 tataccgaac cgttattaca cgcctggcgt ttacgcgaaa aagaaagtca ttaatagcgt
    18961 caaaaaagcc ccggcaagtt ttattgtcgg ggctatgata gtaactgcgg tagtacattt
    19021 atcccggaaa taaccggagc gattatcagg gtttaacaat attaaatacc cgtagcgcat
    19081 tactagcggg cctaaaacaa caatatggac atcttaacga tactctgcgc ctgcgtgtgc
    19141 ggaacagaaa cccttacccg atatacttgt tatgccagag gtagcaattg cgttagcggc
    19201 gtactgttgc tgtaaagtat aacgcctata ttttacagtt cagtcctctt ttgcttatta
    19261 caaccttaag tatatcaagc atataaagat aataagagac tgaacaatat ggttacctgg
    19321 acccaaatgt atatgccgat gggaggactg gggctatccg ctctggtcgc cctgatcccg
    19381 ataatattct tcttcgttgc actcgcggta ttacgtctga aaggacatgt tgctggagca
    19441 ataaccctta tattatctat cctgattgcg atattcgcct ttaaaatgcc gattgatatg
    19501 gcatttgctg ctgcgggcta tggctttatt tatggattat ggccaattgc gtggattatt
    19561 gtcgcggcgg tgttcctgta taaattaacc gttgccagcg ggcagttcga tattatccgc
    19621 agctcggtta tctccatcac cgacgatcag cgtttgcagg tgttactgat tggtttctcc
    19681 tttggtgcgt tgctggaagg agctgctggc tttggtgcga cggtggcgtt tggtgcgttg
    19741 ggcgtgccga ttctggtcgc cggtcaggta acgggaatcg atccgttcca cattggcgca
    19801 atggcgggac gtcagttacc gttcctgtcg gttcttgtgc cgttctggct ggtagcaatg
    19861 atggacggct ggaaaggggt gaaagagacg tggccagcgg cgctggttgc tgggggaagc
    19921 ttcgctgtca ctcagttctt tacctctaac tatattggtc cggaactgcc ggatattact
    19981 tcggcgctgg tgagtatcgt ctcactcgct ttattcctta aagtctggcg gccgaaaaat
    20041 accgaaacgg caatcagcat ggggcaatcc gcaggtgcga tggtggtaaa taagccatct
    20101 tctggcggtc ccgtgccttc agaatatagt ctggggcaaa tcattcgtgc gtggtcaccg
    20161 tttttaatct taacggtgct ggtcaccatc tggaccatga agccgtttaa agcgttattt
    20221 gctccgggcg gcgcgtttta ttcactggtg attaatttcc agatccctca tttgcatcaa
    20281 caagtgttga aagcggcacc cattgtcgcc caaccaacgc caatggatgc ggtgtttaaa
    20341 ttcgaccctc tctcggctgg cggcaccgct atttttattg cggcgattat ctctatcttc
    20401 atcctcggcg tgggcatcaa gaaagggatt ggcgtctttg ccgaaatgct aattagtctg
    20461 aagtggccga tattgtcgat tggcatggtg ctggcattcg ccttcgtcac caactattcc
    20521 ggcatgtcca ccacgctggc gctggtactg gcaggtacag gcgtgatgtt cccgtttttc
    20581 tcaccgtttc tcggctggct gggcgtattc cttaccgggt cggacacttc ttcaaacgcc
    20641 ctgtttggtt cactgcaatc gaccacggcg cagcaaatca acgtctctga cactctgctg
    20701 gtggcagcaa ataccagcgg cggcgtaact ggcaagatga tctccccgca atctatcgcc
    20761 gtggcctgcg ccgcgacggg catggtgggc cgagaatctg aactgttccg ctacaccgtg
    20821 aagcacagtc tgatttttgc cagcgttatc accctgctgc aggcgtatgt gtttaccggg
    20881 atgttagtct cgtaaatcaa taaagccgga tgaaggtaaa tatcacccgg ctttccattt
    20941 gtctgtcgtt tatctcgcca gggaaccgtt tttacgattg gcttctttat acgtagtctc
    21001 cgttttacga tgcgaattgt ttaatattta tacaacttgg cttctgtgct ggttataaag
    21061 tacccacttt catgagatca attttagtat gttattacat tgcttcatct gttgctttca
    21121 tgacgaatgg cgttttttct cttatttata tttttcacta gaacaatgag tagatcaaaa
    21181 ttcccaaaac tcccaaaaac ataagattat tcgcaaagct gattttgcag aataatgaga
    21241 acttttaaga tttgtcgagg atcaatttta agcaatttat ctccacttgt tacataaaag
    21301 atgccgttag gttaataagt taatcttgct ataacagtgt tcgttactgc ctattaataa
    21361 agatatttgg gatagggtaa cgtggatgag gattcgaaat catccgtctt gcggaaagtg
    21421 aaatgttggc agatgttaat attatgtatg tattttaact ccaacaacac accaatcatt
    21481 ttgttagaaa taagaaaatt tatggttaaa taatttggtg attatgaagc attaaatcgt
    21541 aatgccaaat attgttcgct ttggagagta ttattgcctg tttgtgtctg ttgagcagaa
    21601 aaatcatctg aatttgaata cttttattag gtgtgaataa atgaaaaaat cattaaaatc
    21661 attaaaatca ttaaaatcat taaaatcatt aaaatcatta aaatcattaa cttatatgaa
    21721 tatttcagag ttggagttcg aatgttgaca cctgagtatt tcctgctaaa aatagccctg
    21781 ctttacggat ggttgtattg caataacacg aaagtagtaa gagattaaag gtatctgtca
    21841 gcgataactc tgcaagtgat gacattttgt aaaccaaata atgtaaatca gcaatgtatc
    21901 ttttcactta attataacaa atgtggtatt cactaagaat ctgatacgag cttacatcac
    21961 gactatttta gtctcatcgc gggatacagg gatgcaccga tggatctcag cacaccgtaa
    22021 tgcaatacct gaacaaatag aacagtaaag tggttcagta ttaatgaaag ctcttatttt
    22081 ccgttgggaa aacttaaaat agatgagaag gctttttgat ttcttatgtg atctcatttc
    22141 gagtattgag aaatttgtga tttttaaatt tttcaataag ccctgtaagg gattggggtt
    22201 ttatgagaaa taaagagacg tattgtcttt gtttaaacgg tacg
//
LOCUS       DADLTY010000060        22193 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.060, whole genome shotgun sequence.
ACCESSION   DADLTY010000060 DADLTY010000000
VERSION     DADLTY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.060"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..295
                     /locus_tag="JN334_001900"
     CDS             <1..295
                     /locus_tag="JN334_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438054.1"
                     /translation="CPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEF
                     GPIEELRQKHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            297..1151
                     /locus_tag="JN334_001901"
     CDS             297..1151
                     /locus_tag="JN334_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY7438055.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            1194..1808
                     /gene="dmsD"
                     /locus_tag="JN334_001902"
     CDS             1194..1808
                     /gene="dmsD"
                     /locus_tag="JN334_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HAY7438056.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            1967..3259
                     /gene="clcB"
                     /locus_tag="JN334_001903"
     CDS             1967..3259
                     /gene="clcB"
                     /locus_tag="JN334_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HAY7438057.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(3212..3907)
                     /gene="bioD"
                     /locus_tag="JN334_001904"
     CDS             complement(3212..3907)
                     /gene="bioD"
                     /locus_tag="JN334_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY7438058.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4032..5252)
                     /gene="mlc"
                     /locus_tag="JN334_001905"
                     /gene_synonym="dgsA"
     CDS             complement(4032..5252)
                     /gene="mlc"
                     /locus_tag="JN334_001905"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HAY7438059.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(5387..6325)
                     /locus_tag="JN334_001906"
     CDS             complement(5387..6325)
                     /locus_tag="JN334_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7438060.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAELAELAELAKEPFVFFDPHVGT
                     GLYDDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVP
                     IAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            6432..7685
                     /locus_tag="JN334_001907"
                     /pseudo
     CDS             6432..7685
                     /locus_tag="JN334_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            8082..8417
                     /locus_tag="JN334_001908"
     CDS             8082..8417
                     /locus_tag="JN334_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HAY7438061.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            8510..8593
                     /locus_tag="JN334_001909"
     CDS             8510..8593
                     /locus_tag="JN334_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438062.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            8693..9514
                     /locus_tag="JN334_001910"
     CDS             8693..9514
                     /locus_tag="JN334_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAY7438063.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(9553..9882)
                     /gene="mdtI"
                     /locus_tag="JN334_001911"
     CDS             complement(9553..9882)
                     /gene="mdtI"
                     /locus_tag="JN334_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HAY7438064.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(9869..10234)
                     /gene="mdtJ"
                     /locus_tag="JN334_001912"
     CDS             complement(9869..10234)
                     /gene="mdtJ"
                     /locus_tag="JN334_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HAY7438065.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            10645..11679
                     /gene="tqsA"
                     /locus_tag="JN334_001913"
     CDS             10645..11679
                     /gene="tqsA"
                     /locus_tag="JN334_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAY7438066.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(11704..13092)
                     /gene="pntB"
                     /locus_tag="JN334_001914"
     CDS             complement(11704..13092)
                     /gene="pntB"
                     /locus_tag="JN334_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HAY7438067.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(13103..14635)
                     /gene="pntA"
                     /locus_tag="JN334_001915"
     CDS             complement(13103..14635)
                     /gene="pntA"
                     /locus_tag="JN334_001915"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAY7438068.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            15159..16103
                     /locus_tag="JN334_001916"
     CDS             15159..16103
                     /locus_tag="JN334_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7438069.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            16289..17671
                     /locus_tag="JN334_001917"
     CDS             16289..17671
                     /locus_tag="JN334_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7438070.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            17708..18430
                     /gene="folM"
                     /locus_tag="JN334_001918"
     CDS             17708..18430
                     /gene="folM"
                     /locus_tag="JN334_001918"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HAY7438071.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(18427..18762)
                     /locus_tag="JN334_001919"
     CDS             complement(18427..18762)
                     /locus_tag="JN334_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HAY7438072.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGIMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            18891..19634
                     /gene="rstA"
                     /locus_tag="JN334_001920"
     CDS             18891..19634
                     /gene="rstA"
                     /locus_tag="JN334_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HAY7438073.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            19859..21178
                     /locus_tag="JN334_001921"
     CDS             19859..21178
                     /locus_tag="JN334_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HAY7438074.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            21357..>22193
                     /gene="rstB"
                     /locus_tag="JN334_001922"
     CDS             21357..>22193
                     /gene="rstB"
                     /locus_tag="JN334_001922"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HAY7438075.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSE"
BASE COUNT         5345 a         5641 c         5411 g         5796 t
ORIGIN      
        1 ttgtccgtac ggcgcaccac agtacaatgc tgaaaaaggg cacatgacga agtgcgatgg
       61 ttgttattcg cgcgtcgccg aggggaaaca acccatatgc gtcgaatcct gcccgctgcg
      121 tgcacttgag ttcggtccaa ttgaagagct gcgccagaag cacggcactc tggctgccgt
      181 cgcgccactg ccgcgcgcgc atttcacaaa acccaatatc gttatcaaac ctaacgccaa
      241 cagccgcccg accggcgata ccactggtta tctggctaat ccggaggaag tgtaaaatgg
      301 gaaatggatg gcatgaatgg ccactggtta tctttaccgt attgggtcaa tgcgtagtag
      361 gcgcgctgat tgttagcgga attggctggt tcgccgccaa aaatgacgca gaccgtcagc
      421 acattgttcg tggcatgttt ttcctctggc tgttgatggg catcgggttt attgcctctg
      481 tcatgcacct gggatcgcca ctgcgagcgt ttaactcgct gaatcgaatc ggagcttctg
      541 gtctgagtaa cgagattgct gccggttcca tattttttgc cgttggcgga ctatggtggc
      601 tggtggcggt catcggtaaa atgccgcaag cgttggggaa actctggtta cttgtcagca
      661 tggcgctcgg cgtcattttc gtctggatga tgacctgcgt gtatcaaatc gacaccgtgc
      721 caacctggca taacggttac actacgctgg ctttcttcct gacggtattg ttgagcggtc
      781 caatacttgc tgccgcaatt ctgcgggcag cccgcgttac atttaatacc acaccatttg
      841 ccatcattag cgttctggcg ctgatcgctt gtgccgaggt gattgtgctg caaggtttgt
      901 ctctggcgtc tattcactca tccgtgcaac aagccagcgc actggtacca gattacgcct
      961 ccttacaggt ctggcgtgtg gtattgttat gcgcaggtct tggatgctgg ctttgtccgc
     1021 tcattcgtcg ccgtgaacct cacgttgccg gacttgttct ggggctaatc ctcatacttg
     1081 ttggtgagat gatcgggcgt gtgctctttt atggcctgca catgacagta ggaatggcta
     1141 ttgcaggtta acaaattggt gcggggcaac ccgcacattt caggatgtta ggaatgaccc
     1201 atttttcaca gcaagataat ttttctgtcg cggcgcgcgt gttgggcgcg ctgttttatt
     1261 acgctccaga gagtgcggaa gccgcacctc tggttgcggt actcaccagt gacggctggg
     1321 aaactcagtg gcctctacca gaagcgtcat tagcgccgct ggtgacggct tttcagacac
     1381 agtgtgaaga gactcacgcc caggcctggc agcgtttgtt tgtcggcccg tgggcactgc
     1441 cgtctccgcc gtgggggtcg gtctggctgg atcgcgaatc tgtgctgttt ggcgattcaa
     1501 cattggcact tcgtcagtgg atgcgcgaga aaggcattca gtttgaaatg aagcaaaacg
     1561 aaccggaaga tcattttggt tcactgctgt tgatggctgc gtggttggcg gagaatggtc
     1621 gccagacaga atgcgaagaa ttgctggcat ggcacctttt tccgtggtca acacgttttc
     1681 ttgatgtttt tatcgaaaaa gcagaacacc ctttctaccg tgcactgggt gaactggctc
     1741 gcttaacgct ggcgcaatgg caatcacaac tgttgattcc tgtcgcggtt aaaccactct
     1801 ttcgatagga tcaccgtaat attgccggat ggtgacgatt acgcgcgatt cggcaataat
     1861 acgttacaat gtcacgtttt tttatctctt aaagcacgca ctgcttttgc ggttggcctc
     1921 ttttgccgca aaatagtcgc ccgtgtttca ttgcccattt ctgctcatgc atcatctaca
     1981 catctatccg gatctgcgca ctatgttccg ccgtctgctt atcgcaacag tcgtcggtat
     2041 tctcgctgtc tttgccgttg ccgggtttcg tcatgcgatg ctgctactgg agtggttgtt
     2101 cctcaataat gactccggca gtctggtcaa tgcagcgaca aacctttcct cctggcgacg
     2161 gctgctaact ccggcgctcg gcggactggc ggcgggtttg ttgctgatgg gctggcagaa
     2221 atttacccaa caacgccctc atgcgccgac cgattacatg gaagcgttgc aaaccgatgg
     2281 acagttcgat tacgcagcaa gcctggttaa atcgcttgcc tcgctgctgg tagtaaccag
     2341 cggcagtgca attggtcgcg aaggtgcgat gattctttta gctgcccttg ccgcctcctg
     2401 ttttgcccaa cgttttacgc cacgccagga atggaaatta tggatcgcct gtggggccgc
     2461 ggcgggaatg gctgcggcct atcgtgcccc gcttgctggc agtttattta tagccgaagt
     2521 gctgtttggc actatgatgt tggcctctct cggcccggtg attatttccg ccatcgtggc
     2581 atggctggtt agcaatctga ttaatcatag cgacgcgtta ctctacaacg tacaactctc
     2641 agtgacggtt caggctcgtg actatgcgct gattatcagt acaggtgtgc tggcaggtct
     2701 gtgcggacca ctgttgttaa cgttaatgaa cgcctgtcat cgtggatttg tgagtctcaa
     2761 acttgcgccg ccctggcaac tggcactagg cgggttgatc gtgggtctgc tttccctgtt
     2821 cacacctgca gtgtggggca acggctatag caccgtacaa tcctttttaa ccgccccacc
     2881 actgttaatg atcattgccg ggatcttcct ttgtaaactg tgtgccgtgc tggcgagtag
     2941 cggttccggc gcacccggtg gtgtctttac accgacgcta tttatcggtc ttgccattgg
     3001 catgttgtat ggtcgtagcc tgggattatg gttccctgat ggcgaagaaa ttacgctttt
     3061 actcggattg accgggatgg cgacactgtt ggcggcaacc acgcacgcgc cgattatgtc
     3121 gacgttgatg atatgtgaaa tgaccgggga gtatcagcta ctccccggtt tattgattgc
     3181 ctgcgtaatt gcgtcggtaa tttcgcggac gttacaccgt gactctatct accgccagca
     3241 cactgcgcag catagctaag cggatgtatt gccccagttc acgctgttcg gcgcgcggca
     3301 gataaggcag ttcaccaatg agcggtgccg gaagtttttt acccagcaca tcaatgattt
     3361 ccgcataatg cgccagtcct gggttgattc ggttagccac ccagccaatg agcggcagcc
     3421 cgtcgttggc gatcgcctga gctgttagca gtgcatggtt aatgcaacct tcctgaatac
     3481 cgacaaccat caacaccggc agttgttcct gcactaccca ttcagagagt ggacgcaaat
     3541 cattcatcag actgcgccag ccgccagtcc cttctaccac gacatgatcg actttatcgg
     3601 tcaggtttgc caggccgttt gaaatgaggg tgtaattgat tgggcaactg tgcgccacgc
     3661 tactttcttc ttcgcttaac gcgataggat taactgcttc ataaggcagt tcgatggttg
     3721 aaacactctg caacaccaag gcatctttat tacgcagccc ttcgggtgtc tctttgctcc
     3781 ccttcgctac gggtttatat cccgcaaccc tttttccctg ggaggctaac gcttgtagca
     3841 atgcgcggga aaccaccgtt ttccctacag aagtgtctgt accggtaata aagaaacgct
     3901 tcagcatcac taactccacc gttatgcttc acaaatataa actaggaaaa taattaacct
     3961 tgaaagtcta agttatgctt tcctggccca aattgagata gcgcaaattt tggtagaaca
     4021 gttaaaaaat gttaaccctg caacagacga atcaacaaag aaccgttata catcgcgtct
     4081 tttaccagtg cagcgcctgc catcgtgccc tggttagaaa actgagtact ctcaacgcta
     4141 atgtgctgac tatacgcagg aagggcctgc tgacggatgc tgtctgagat gaccgggaag
     4201 aggatatctg ccgctttact taacggtgag ccaatcagta ttttttgtgg gttaaataaa
     4261 ttcaccatga tggcaagaat gcgccctaca tgcgcgccca ccccgataat gatgtctttt
     4321 gccagtagat cgccgcgcaa tgccgcctga cacaatgagt ccacggttaa cggttgccca
     4381 tgtaacatcg agctcatgga ttgattgaga cgcagctgtg ccagctcaag aatactgtcc
     4441 acactggcga tggtttcgag gcagccgtgg ttcccgcaat aacagcgttt accatatggg
     4501 tcgacctgtg tgtggcctat ttccacgaga ctactgctgc ctgcgtgtag cagatgacca
     4561 tcggtaatga cgcccacccc cacgttgtga tcgataacca cctgaatcac atcgcgcgcc
     4621 ccgcgtgagg caccaaacaa ggcctctgcc atcgtccatg cgctgatatc atgctgaata
     4681 taaaccggta cgccggtatg ctgctccagc gcctcgccga gcggcatctc ttttacatcc
     4741 tcgtagaacg gcatgcgatg tacaatacca ttttccgtat caataattcc cggcagggtt
     4801 atggcaatcg aagttagacg ctcaagtttt ttctggtggc ggataaaaaa ctgatcgata
     4861 tgggaaatga tacgatccag caatggcaag tcatctttta acgccagttc ctgcgactct
     4921 tccaccacca gtttgctgct cagatcgcgc agagcaagga aaatctcccc gcgactaatg
     4981 cgcagagaaa gatagtgcca ggcttcagtt tcaaccacca gccccaccgc cggacggcca
     5041 cggttccccg cttctttgat ttccagctct cgcaccaggt gtgcttcgag catctcacgg
     5101 acaattttag tgatactggc aggagccagc tgcgccagac gggaaagatc gatacgcgag
     5161 actggaccaa gctgatcaat caggcgataa accgcgcccg cgttggtctg ctttatttga
     5221 tcaatgtgcc caggctggtt ttcagcaacc accgcatact ccctatattt tcgcgctccg
     5281 aaataatctg taggctatgg tgaagcactt caatacgtgt cgtcaaattt ttacttaggc
     5341 atgtgattaa cagcacattt ttcgggcttt ttcgctgaaa tttccctcac ctgagagcat
     5401 tcagcagatg aatacgaaag ttacgcgcag ctggactttg ttcatgatgt ttcggccaga
     5461 ccaaccacat ttcagaaacc gcatcctctt cagcaatcgg cacccagcgc atttcgttga
     5521 gctgaacacg tttaaatgac gcaggcaaaa ttgaaacacc cagaccggcg gaaaccagac
     5581 cgatgatggt cattgcctcg cccacctcct gagtgatgac gggcgtcaaa tggtaacgtc
     5641 gcatcagtcc gagaatatcg tcatacagcc cagtcccgac gtgcggatca aaaaagacaa
     5701 agggttcttt cgccagttca gccagttcag ccagttcagc cagttcagcc agttcagcca
     5761 gttcagccag tgttacactc ggattatttg ccaggggatg atcgtacggg atcatcgcca
     5821 taagcggttc atggacgatg actgcgtgtt caagcgtctc cggtaacgct gtgttacgca
     5881 gcaatcccat atccagcgtt ccttcaatga gcggagcgat ttgctcgcga gtgttcattt
     5941 cgcgggtttg taaatggaca tcaggataat cacggcgaaa cagcgataac gtatcggaca
     6001 ccgcccgaat aaaaggagcg gacgaagtaa aaccaatacg caactccccc gcttcaccct
     6061 gatgcagtct ttcagcgcga gcggcagcgt catccaccat agacaggatt tgccgactat
     6121 ctgcaagaaa ctgttttcct gctgccgtca gcaatacact gcgattagtt cgtgccagca
     6181 gtcgggcacc gatttgttgc tccagtgcct gaatctgctg acttagcggc ggttgcgaaa
     6241 tattcaggcg ggcagccgcg cgcccaaaat gcagctcttc cgcaacagca acaaagtaac
     6301 gcagatgacg aagttcaata ttcatattta aaacatctta tttgagatta ttaatatatt
     6361 agacagaaca attcgatttt cctaccctat gtataagcct gatccacagg catatttagc
     6421 aaggatttca agtgagccgt actacaactg ttgatggcgc tccggcaagc gacactgaca
     6481 agcaaagcat ttctcagcca aatcaattta ttaaacgcgg tacgccgcaa tttatgcgcg
     6541 tcaccctggc gctgttctct gccggactgg caacctttgc acttctctat tgtgtgcagc
     6601 ctatccttcc ggtgctttcg caggagtttg gcttaacgcc cgcgaacagt agtatttcac
     6661 tgtccatttc cacggcgatg ttggctatcg gtttgctatt tactggcccg ctatccgatg
     6721 ccattggtcg caaaccagtg atggtcacgg cgctactgtt ggcctccatt tgtacgttac
     6781 tttcgacaat gatgaccagc tggcacggca ttttgattat gcgcgccttg attgggcttt
     6841 cgttaagtgg cgtggcagct gttggcatga cttatcttag cgaggaaatc caccccagtt
     6901 tcgtggcctt ttcgatgggg ttgtatatca gcggcaactc aattggcggc atgagcggac
     6961 gcttaattag tggtgtcttc acggactttt tcaactggcg aattgctctg gcggcaatcg
     7021 gttgtttcgc gctggcctcg gcgttgatgt tctggaaaat cctccctgaa tcacgccatt
     7081 tttgcccgac ttcgctgcgc cctaagacgt tatttatcaa ctttcgtctg cactggcgtg
     7141 accggggatt accgttattg ttcgcagaag gctttttgct gatggggtcg ttcgtcacgc
     7201 tgtttaatta catcggctat cggttgatgc tttcccccta gcatgtcagt caggccgtgg
     7261 ttggcttatt atcgctggct tatttgaccg gtacatggag ctcacccaaa gccggaacca
     7321 tgaccacccg ctatgggcgc ggtccggtga tgttgttttc gacgggagtt atgccgtttg
     7381 gtttactgat gaccttattt agctcgctgt ggctgatctt tgccggaatg ttactcttct
     7441 cagccggatt cttcgcagcc cactcagtag ccagcagctg gatcggcccc cgcgcaaaac
     7501 gcgctaaagg ccaggcctcc tcgctgtatc tgttcagtta ctatctgggg tcgagtattg
     7561 ccgggacgct gggtggtgtt ttctggcata actatggctg gaacggcgtc ggcgcattta
     7621 ttgctctgat gctggtcatt gctctgctgg tcgggacgcg tttgcatcgt cgtctgcacg
     7681 cctgaaaaat aagtccggac tgcggtaaat acccgtccgg acttattgcc tgctcaaacc
     7741 aacgttaata gccatcctaa aatagacgaa gcgccagcca attcccgcag cgcatctagc
     7801 gtcatcagga ttataagtac ccaaataaac ggattcattt tgctgtgtgt catttattac
     7861 tgatgcgcag ttattctacc gctttgtaag tagtataata gttaacccga tcaagactac
     7921 tattattggt agctaaattt tccttaagtc acaatacgtt attatcaacg ctgtaattta
     7981 ttcagcgttt gtacatatcg ttacacgctg aaaccaacca ctcacggaag tctgccattc
     8041 ccagggatat agttatttca acggccccgc agtggggtta aatgaaaaaa caaattgagg
     8101 gtatgacaat gaaaaaagta ttagctctgg ttgttgccgc tgctatgggt ctgtcttctg
     8161 ccgcctttgc tgcagagact gcgaccacac ctgctccgac tgcgacgacc accaaagcag
     8221 cgccagcgaa aactacacat cataaaaaac agcataaagc agcacctgcc cagaaagcgc
     8281 aggcggctaa aaagcatcat aaaaatacga aagctgaaca gaaagcccct gaacaaaaag
     8341 cgcaggcagc gaagaaacac gccgggaaac acggccatca gcaaccggca aaacctgctg
     8401 cacaacccgc agcgtaagtt ttcaacagta atgctggcgc gccccctcgc gcctgaaaat
     8461 tacggtgcta agcgggtaac gtttagcacc gcctttagcg gagggtagta tggtgggccg
     8521 ttatcgcttt gagttcattc ttatcatcct tattttatgc gcactgattg ccgcccgttt
     8581 ttatctttcc tgactgtagt tatctgattt tactcccact ttcatcccgt cccgtctata
     8641 gtatttatga gggtttgctt ttaataatca taattaccca ccagagtgtg atatgcgtac
     8701 aaccattgct gtagtgttgg gtgcaattag tttgacgtct gcttttgtgt ttgcagataa
     8761 accagacgtt gccaaatcgg caaacgatga ggtcagcacc ctgttttttg gtcatgatga
     8821 tcgtgtgcca gtgaatgaca cgacccaatc accgtgggat gcggttgggc aactggaaac
     8881 ggccagcggc aatttatgta cggcgacgct gattgcaccc aatctggcat taacggcagg
     8941 acactgttta ttgacgccgc ctaagggtaa agcggataag gcggtagcgc tgcgttttgt
     9001 gtcaaataaa ggtctttggc gctatgagat ccacgacata gaaggccgcg ttgatccgac
     9061 actgggaaag cggttaaaag cagatgggga tggttggatt gttcctcccg cagctgcacc
     9121 gtgggacttc ggattgattg tgctacgtaa tcccccttct ggcattacac cgttgccgtt
     9181 atttgaggga gataaagccg cgcttactgc cgcattaaaa tcggctggtc gtaaagtgac
     9241 tcaggcggga taccctgaag atcatctcga tacgttatac agtcatcaaa actgtgaagt
     9301 gactggctgg gcgcaaacgt cggtgatgtc acatcagtgc gataccttgc cgggtgacag
     9361 cggttcgcct ctgatgttgc ataccgatga cggctggcaa ttaattgggg tgcaaagttc
     9421 ggctcctgcc gcgaaagatc gctggcgcgc cgataaccgg gccatttctg ttaccggttt
     9481 tcgcgacaag ctggatcaac tgtcgcaaaa ataatgttca aacgctgccc gacagcgcgg
     9541 gcagcgtctt catcaggcaa gtttcaccat gatcattcca gccaacagca agaccaggcc
     9601 aatccagcct ttacgattta accgttgacc aaacaagatc caacctgcgg ctaacgtggc
     9661 ggcaatacca aacccgcccc acaatgcata aacgacagac aagtcgatcc ctttaacggc
     9721 ctgagaaagc gcactaaagg ccgccagcac cgccgccagg gagagcaagc caaatatttt
     9781 gcgacgaaag ccgtcagaaa atttcaaaaa gacgttagca acgatttcca gcacgattgc
     9841 caatgccagc caggcggcgt gaacccattc aaactgcgcc atggttcacc tccagttcag
     9901 gtttacgcgc tttacgggta cctgatttga tcaacactat cccggcgacc agggtggtta
     9961 acccggcaat tttcatcagc gataaacttt cgtcgaataa caaaacgcta aacaaggtaa
    10021 taaataaaat accgatacct tcccacagcg cataagctac gcctaaggcg atttttttaa
    10081 cggcgaaaga gagaaatata tacgacagag aaatcatcac cagcattaaa ataaagccgc
    10141 catttccctc actgacgctc gcccatttca ttgacagcgt accggtaatt tctgtagcaa
    10201 tagccagacc taataaaatc caataaatat acattgtcct tctcctgcaa gagaactatt
    10261 ttaattttcg cttaattcag cgaaaccaaa gtaaagtggt caagccctgc gcatagggca
    10321 agcttaggca gaagaaagga ctaaagcgcg ttgcgccagt gctgctcacc tacgagcaag
    10381 atagtggatg aggtacgaag atgggtaaat gtagaaaata acgtcctgaa caaattgtcc
    10441 ataatattac aattatccgc agtgttgctt ctcgtcatcg cagatgataa ttgtcctcgg
    10501 tagttgaaca cgcctgattt gtatcatagc ttaagaatta actcaaaata ttttcacttc
    10561 tttacctgag cggtttgatt ttcgttatga tgacggagcg aaaaagacat tattattagc
    10621 aaaggaagaa aaacggggac aagcatggca aagccgatca tcacgctcaa tggcctaaaa
    10681 atcgtcatta tgttgggaat gctggtcatt attctctgcg gtatccgttt tgccgccgag
    10741 atcatcgtgc cgtttattct cgcattattt attgctgtta ttcttaaccc gctggtgcaa
    10801 catatggtcc gctggcgtgt gccgcgtgta ctggcagtgt cgattttgat gacaatcatc
    10861 gtgatggcga tggtgttgct attagcttat ctgggttccg cgctcaacga gttgacgcgg
    10921 acgttaccgc aatatcgcaa ctctattatg acgccgctgc aagctcttga gccgttgttg
    10981 caacgcgtag ggattgacgt ctcagttgac caactggcgc attacattga tccgaacgcg
    11041 gcgatgacgt tgctcaccaa cttattgacg cagttatcta atgccatgtc atcaatattt
    11101 ttattgctgc tgacggtgct gtttatgctg ctcgaagtgc cacaattgcc cggaaaattt
    11161 cagcaaatga tggcgcgtcc ggttgaaggg atggcggcga ttcaacgtgc gattgacagt
    11221 gtttctcatt atctggtgct gaaaacagcc atcagcatca tcaccggcct ggtcgcctgg
    11281 gcgatgctcg ccgcactcga tgttcgcttc gcttttgtct ggggattgct ggcctttgcg
    11341 cttaattaca tccctaatat tggttcagtt ctcgcggcaa tcccccctat cgctcaggta
    11401 ctggtgttta atggcttcta cgaagcgttg ctggtgctgg cgggatatct gctgattaat
    11461 ctggtcttcg gcaatattct ggagccgcgc atcatgggac gtgggctggg gctttcgaca
    11521 ttggtggtat ttttgtcgtt gattttttgg ggatggttgt taggaccggt gggtatgctg
    11581 ctttccgtgc cgttgacaat tattgtcaaa attgcgcttg aacaaacagc gggaggtcaa
    11641 agcatcgccg ttctgttaag cgatctcaat aaagagtgac ggcctccgca gaggccgtca
    11701 gggttacaga gctttcagga ttgcatccac gctggctttg gcgtcaccaa acagcatgtg
    11761 ggtgttttcc ttgaagaaca gcgggttttg cacaccagca tagccagtgt tcatcgaacg
    11821 tttaaagaca atcacgttct gcgctttcca cacttccagc acaggcatac cagcaatcgg
    11881 actcttcgga tcatcctgcg ccgccgggtt aaccgtatcg ttagcaccaa tcaccagtac
    11941 ggtatcggta tcagcaaagt catcgttgat ctcgtccatt tccagcacga tgtcatacgg
    12001 tacttttgct tcagccagca atacgttcat atgtccaggc aaacgccccg caaccgggtg
    12061 gataccgaaa cgcacattaa taccacgagc gcggagtttc tcggtaattt cagcgacagg
    12121 atattgcgcc tgcgcgactg ccatgccgta ccccggagta atgatcactg aatgggagtt
    12181 tttcagcagt tccgctgtct cttctgcggt gatttcgcgg tgctcaccca cttcctgatc
    12241 atcgccagta gaagagccgt cggtgccgaa accacccgca ataacgctga taaaggaacg
    12301 gttcatcgcc ttacacataa tgtaagaaag gatagccccc gaagaaccga ccagcgcacc
    12361 ggtcacaatc agcaggtcgt tgctgagcat aaagcccgca gccgcagccg cccagccgga
    12421 gtacgagttc agcatcgaca ccaccaccgg catatctgca ccaccgatgg aggcgactaa
    12481 atgccagccg aataccagcg caatggcggt cattatcagc aatgccagca cttgcaggcc
    12541 gacgctgtcc gtgcgaacaa atacaatcag cagcaagaag gaaacgacca gagccgccag
    12601 gttcattttg tgacggtttg gcagcatcaa cggtttagac gaaatcttgc cacacagttt
    12661 gccgaacgcc accaccgagc ccgtgaacgt taccgccccg atgaagatac cgaggaacac
    12721 ttccgtcagg tgaatattga ccagaatcgg tgccattccc gcgtcatgat gcagataact
    12781 gttaaagcca accagcactg ccgccagacc cacgaagctg tgcaggatcg ccaccagttc
    12841 tggcatttcg gtcatttcaa ctttcttcgc cagccggata ccaattgccc caccaatgac
    12901 catcgccagc aagatccagc caacattacc cgtatccggt ccaaaaatgg ttgcgattaa
    12961 cgcaatcgcc atcccggcga taccgaagtt gttaccctgg cgagacgttt catgtttcga
    13021 aagaccggcc agactgaaga taaacaggat cgcggcaaca atgtatgcag ctgtaactaa
    13081 tcctccagac atatgttacc ccttaatttt tgcggaacat tttcagcatg cgctgagtca
    13141 cggtgaagcc accgaaaata ttaatgctgg ctataagcac cgcgataaaa ctaaggaagc
    13201 taacccagcc tccctggcca atctgcaaca gtgctccgac aacaataatc cctgaaatcg
    13261 cgttggtaac cgacatcaac ggtgtatgca gcgcgtgcga tacattccac accacgtaat
    13321 aaccgacaac gcaggccagc gcgaaaacgg tgaagtgccc aagaaactct ttcggcgcaa
    13381 cgcttgacag ccaaccgaac aggatgattg ccagcgccat caacgcgtat ttacgccacg
    13441 gtgagcaggc acatttttcc tcagttttca cttccggtgc cgctttttgt gccgcctgcg
    13501 gctgagctga tacctgaatc ggcggtgccg gccaggtaat ttcgcccgca cggatcacgg
    13561 tcacgccgcg aatcaccaca tcatcaaaat caacagtgat attgccgtct ttctctttgc
    13621 acaacagttt cagcagatta acgaggttag taccgtaaag ctgtgaggat tgcgtcggca
    13681 gacggcccgg aagatcggta taaccaatca ctttgacacc attttccgta ctgaagattt
    13741 cacccggcac gatgtattca cagttgccac cgttttgggc tgccaggtcg acaatcacac
    13801 tgcccgcctt catggagtca accatttcac gggtaattag cttcggcgct ggtttgcctg
    13861 gaataagcgc ggtggtgaca atgatatcga cctcttttgc ctgggcggca aagagttcca
    13921 tttccgcttt gatgaacgcg tccgacatca ctttggcata gccatcgccg ctgcccgctt
    13981 cctctttaaa atccagctcg aggaattccg cgcccatact ttgaacttgt tctttcactt
    14041 ccgggcgggt gtcgaatgca cgcacaatcg cgccgagact gtttgctgcg ccaatggcgg
    14101 ccaaacctgc aacaccggca ccaatcacca tcacttttgc cggtggcact ttcccggccg
    14161 cagtgatttg cccggtaaag aagcgcccaa attcatgtgc cgcttcaaca atggcgcgat
    14221 aaccggcgat attcgccatc gagcttagtg cgtccagcga ttgtgcgcgt gagatacgcg
    14281 gcacagagtc catcgccatc acggtcacgt tacgttccgc aagtttttgc attaattccg
    14341 gattctgcgc aggccagata aaactcacca gcgttgtccc gggattcagt aacgcaattt
    14401 catcatctaa cggcgcattg accttcagaa tgatctctga ctgccagacg ctattccctt
    14461 ctacaatttc agcgcccgct tgcacaaacg ctttatcgtc aaaacttgcc agttgacccg
    14521 cgccgctctc taccgcgacg gtaaaaccca gtttcagcag ctgttccact gtttttggcg
    14581 ttgctgcaac acgggtttca ttggttaacc gttctcttgg tatgccaatt cgcatgatat
    14641 tcccttccat cggttttatt gatgatggtt tgcctgtgtc aggagccaca caagctgctc
    14701 atgtacgagc taaatgttac tccgttaaaa taaattagta acaaacgccc tataacgtac
    14761 tgaaaattat gcctgtgatc tagcgccaaa aatcagtatt tcggcgtgaa ctcgcaaaat
    14821 attaacgatt cagccgtgat agtgggataa acaccttaga acgccggata aagactgata
    14881 attgtcttcg acggtcgggt aaaacgagac atcgccccgg cacgaatcac tacttaacat
    14941 taaattaact tatacaattc agttgcttca gtagtaatga tgctgatacg gctgtttttt
    15001 aagcatagac ggtcatttga gcaggattaa aattggttta aggaatgtga tatgaaaaat
    15061 gacgcagaca gttacaccgt ttaaatgcaa taatcagcca cgtttctcgt taataacaat
    15121 accagtacct ggtttgcgca aggcgaagga ttatttttat gaagcttaag aacaccctcc
    15181 tggcgtcggc actgctttct gctacggcat tctccgttaa cgcagcaaca gaactgacac
    15241 cggagcaagc ggcagcggtt aaaccttttg accgtgtagt ggttaccggt cgttttaatg
    15301 ctattggcga agcggtgaaa gccgtttctc gtcgcgcaga taaagaaggt gccgcctctt
    15361 tttatgttgt cgacacttct gattttggta acagcggtaa ctggcgtgtg gtcgctgacc
    15421 tctataaagc cgatgctgaa aaagcagaag aaacaagtaa tcgcgtaatt aacggtgttg
    15481 tcgaactgcc gaaagatcag gctgttctga ttgaaccgtt tgacacggtc accgtccagg
    15541 gcttctatcg tagccagcca gaagtcaatg atgccatcac caaagcggca aaagcgaaag
    15601 gtgcctactc tttctacatc gttcgtcaaa tcgatgccaa ccagggcggc aaccagcgta
    15661 ttactgcatt catctataaa aaagatgcta agaaacgtat cgtccagagc ccggatgtga
    15721 tcccggcaga ttccgaagca ggacgtgcag ctctggctgc cggtggcgaa gccgcgaaga
    15781 aagttgagat cccgggtgtt gcgactaccg catcaccaag ttctgaagtc ggtcgcttct
    15841 ttgaaaccca gtcatcaaaa ggcgggcgtt acaccgtcac gctcccggat ggcactaaag
    15901 tcgaagaact gaacaaagcg acagcagcga tgatggtccc gttcgacagc atcaaattct
    15961 ctggcaacta cggcaacatg accgaagtct cctatcaggt tgcgaaacgt gccgcgaaga
    16021 aaggtgctaa gtattaccac atcacccgcc agtggcagaa acgtggtaat aacctgaccg
    16081 tcagcgcaga tctgtataaa taactattca gttactccta taaggcggct tgatgccgcc
    16141 tttcgcattt ttagtcacaa atttccccgc gaccattgca tgcagacaca tctcttcgta
    16201 aaatcccgcg cttcagtggc aaactccatt tttatgcgtt ttgccctaat atttattctt
    16261 cttatcacgt tttaatcact ggatatcgat ggaaaagaaa ctgggactga gcgcactcac
    16321 cgcgctggta ttaagctcaa tgctgggcgc gggtgttttc agtctgccgc aaaatatggc
    16381 ggcagttgcc agcccggcag cactgctcat cggctggggt attactggcg ctggcatttt
    16441 attgctggcc tttgccatgc tgatcctcac gcgcattcgt cccgaacttg atggcggtat
    16501 ctttacctat gcccgcgaag ggtttggcga gctaatcggc ttttgttccg catggggata
    16561 ctggctgtgc gcggtcatcg ccaacgtctc ctatctggtg atcgtttttt ccgcgttaag
    16621 cttttttacg gacacgccgg aattgcgcct gtttggcgat ggtaatacct ggcaatcgat
    16681 agtcggtgca tcggctttat tgtggattgt tcactttttg atcctgcgcg gggtgcaaac
    16741 cgctgccagc attaacctgg tggcgacact ggcaaaattg ttgccgctgg gtctgtttgt
    16801 tgtgctggcg atgatgatgt tcaaactgga taccttcaag ctcgacttca ccggacttgc
    16861 acttggcgta cctgtttggg aacaggtgaa aaacaccatg ctgatcaccc tgtgggtttt
    16921 cattggtgtg gaaggtgccg tggttgtttc tgcgcgtgcg cgtaataaac gtgatgttgg
    16981 caaagcgaca ctgctggcgg ttctctccgc tctgggcgtt tacttgctgg taacgctgct
    17041 ttcgctgggt gtggtggcac gtcctgaact ggctgaaatt cgtaacccgt caatggccgg
    17101 tctgatggtg gaaatgatgg gaccatgggg cgaaatcatc atcgctgccg gtttgatcgt
    17161 ttccgtttgc ggcgcgtact taagctggac catcatggcg gcagaagttc cgttcctggc
    17221 tgccactcat aaagcattcc cacgcatttt tgcgcgccag aacgcacaag ctgcgccatc
    17281 cgcctcactg tggttgacca acatctgcgt gcaaatttgt ctggtactca tctggctgac
    17341 cggttccgat tacaacacgt tgctgaccat cgcctcagaa atgattctgg tgccctattt
    17401 cctggtcggc gcgttcttgc tgaaaatcgc aacccgtcca ctgcataaag cggtaggtgt
    17461 cggtgcctgc atttatggct tatggttatt gtacgcctct ggtccaatgc acttgctgtt
    17521 atccgttgtt ctgtatgcac cggggctgct ggttttcctg tatgcgcgca aaacgcatac
    17581 acatgataat gtactgaacc gtcaggaaat ggtgctgatt ggcatgctgt taattgcctc
    17641 agttccggcg acctggatgc tggtgggata acgttcatcc catcgttgga tagcaaagga
    17701 gataacgatg ggtaaaaccc agcccttgcc aatattaatt actggcggag gtcgtcgcat
    17761 cggcctcgcc ctcgcatggc atttcattaa tcaaaagcaa ccggtgattg tcagctatcg
    17821 gacacactat ccagccattg atggactgat taatgcaggt gcgcagtgta ttcaggctga
    17881 tttttcgacc aacgacggtg tgatggcgtt tgccgatgaa gtactaaaaa ccacccatgg
    17941 tctgcgtgct attttgcata acgccagtgc gtggattgcg gaaaaaccgg gtgcgccact
    18001 ggccgacgta ctggcttgca tgatgcagat ccacgttaat accccatacc tgctcaacca
    18061 tgcgctggaa agattactgc gtgggcacgg acacgccgcc agcgatatca ttcactttac
    18121 cgattatgtg gtggagcgcg gtagcgacaa acatgttgcg tatgctgcaa gcaaagcggc
    18181 actggataat atgacccgct cgtttgcccg caagctggca ccggaagtga aagtgaattc
    18241 tattgcgcca tcgctgatcc tgtttaatga acatgatgat gccgaatatc gacaacaggc
    18301 gctgaataaa tcactgatga aaaccgcgcc tggcgagaaa gaagtgatcg acctggtcga
    18361 ttacttactt accagttgct ttgtcaccgg acgcagtttc ccacttgatg gcggtcgtca
    18421 tctgcgttaa tgcagcttta tccagcatat aatcaacacc catgcgctta tcccccaaca
    18481 cgcaacagaa ccaacaaaag cggcgggcaa gcgcattatt ccggtgaaat accacagcga
    18541 caccaggtaa acaaaatagg gaataatcga ccacatacta aaaatgatgg ttgcgcgtaa
    18601 ggcttcaatg ccgcgttcgc tggcaacaat ataatgcgcg ataagcgcaa aggtcgggaa
    18661 aagtggaatc agcccggcga tataataatt tttcgttttt gctaaaacac caatcaacag
    18721 cactaccagc gcaccgagcg cggctttgat taccagcccc atctttttac cttaacactt
    18781 ccataacaag tcatcagtag aatacctgat gaaaacttgt ttagaaacga ttgatagtaa
    18841 gtaaaaacag cgcggtgtat tgtgacgttt ttatatctac cgtgaatgtt atgaacacta
    18901 tcgtatttgt ggaagatgat gcggaagtcg gttcactgat tgccgcgtac ctggcaaaac
    18961 atgatatgca ggttaccgta gaaccgcgcg gcgaccaggc cgaagaaacc attttgcgag
    19021 aaaatccgga tttggtgtta ctcgacatca tgctaccagg caaggacggc atgaccattt
    19081 gtcgtgattt acgcgcaaag tggtctggac cgattgttct tctaacctct ctcgatagcg
    19141 atatgaacca catcctggca ctggaaatgg gtgcctgcga ctatattctc aaaacgacgc
    19201 cccctgctgt tttgctagcg cgtttacgtt tgcatttgcg tcagaatgag caagccacac
    19261 tgaccaaagg tcttcaggaa acgtctctta ctccctacaa agccctgcat ttcggcacgt
    19321 taactatcga ccccatcaac cgcgtagtca ccctggctaa cactgaaatc tcgctctcga
    19381 cagctgattt cgaattattg tgggaactag ctacgcatgc cgggcaaatc atggaccgcg
    19441 atgcattgct gaaaaattta cgcggcgtca gttatgacgg actggatcgt agcgtggacg
    19501 tggctatttc gcggttaaga aaaaaactgc tcgataacgc cgcagaacct tatcgcatta
    19561 aaactgtgcg taacaaaggc tatctttttg cgcctcatgc atgggatagt gacaataaac
    19621 aatcagatga ctaatatttg ttcatctgaa aaagagaaca attaacgaat tcaattaata
    19681 agagcttatt caaattccaa accccctaca tttgatatga aaatgagaaa aaatttctct
    19741 ataatcgcct cgtccctgcg tcttatggcg cagatttttt acaacaaact ctttccggca
    19801 tttcaggtgc tgaatgaggc aagaggctta aaaaacggtt acaactttca catatattgt
    19861 gctttttttg atctgctcat gggggatttt caaaacggat caaaagagcg aatccctttc
    19921 caaaagtgtt tttatcgcga ttttcctggg ggctatcatt ggtatctccc tgcatttcat
    19981 tcctgagaca agcagtgaaa ccgttatcga atggtataat attgctggta acggttatgt
    20041 taatttatta aaacttgttg ccgttccact catattcatt tcgattcttt cagcgattaa
    20101 taagctcgaa aatagcgctg gtattggcaa gatgtcttta actattgttg cctgtatgtt
    20161 gtgtttagtc atgattgcag gtttagttgg cctggctatt gccagtgcaa ttggccttga
    20221 tgccagcgcc tttggtcata tgcaatcctc tttaaccagc gatgacgtct cgaaaacagc
    20281 tgcggtatct ttaccgcgac tcattacgtc attgatccca accaacctct tccttgatct
    20341 gaccggggca agaagtgttt cggttattgg catcgtcatc tttacaattc ttgctggtat
    20401 tgcgctgtta aaagtaaaga aagatgcacc agcagagggc gaaaaattga gcgcggggat
    20461 caacgcgatt caaatatggg taatgaaaat ggtccgtatt gtcattgcgc tgacgcctta
    20521 tggggtaatg gcactaatgg cgaaagtctt ctcaacgtat cagctttcac agtttaccaa
    20581 tttgctcggg ttcatcgtcg cctgttatct ggccgttttt gtgatgttta ttgttcactc
    20641 gggcattttg ctgttaatag gtaaaaaccc cgttaactat tacaagaccg tctggcaagt
    20701 acttaccttt gcgtttgttt ctcgcagtag tgcggcttca attcctctgg ctatttctgc
    20761 attggaaaaa ttgggggttc caaatacgat tgctaatatt gcagcgtctt ttggttccag
    20821 catgggacaa aatggctgcg ctgggattta tcctgccgtg atggtggcga tgattgctcc
    20881 aacaatgggt atcgatccct tctcattgca attcctcgca actcttttac ctgccatcgc
    20941 gcttggctca ataggggtag caggcgtagg tagtggtggg acattcgcgg cgctgattgt
    21001 cctttcaaca cttaatttcc ctgtcgcatt agtagttatt tttattgcca tagagccgat
    21061 cgttgatatg gcaagaacag cgcttaatgt gaatggttca ataatgtccg gtgtaatatc
    21121 agcccgtctt cttaaggata gagagactaa agaacaacaa agcgtcacgt taaattaaac
    21181 cttagcataa taaacaagcc ccggtggaaa attgaattta ctgaatatga atacaccggg
    21241 gatacatccg tctgttccgg acattatctt ccaaacaagg tcttatccag ttggcggttt
    21301 acattgctcc acagtcgtga attaatacga atagtattct gttgcgggcg atgccaatga
    21361 aaaaattgtt tatccagttt tacctgttat tgtttgtctg cttccttgtg atgtctctgc
    21421 tggttgggct ggtgtacaaa tttaccgccg aacgcgcggg caaacagtcg ctggatgatt
    21481 tgatgaacag ttcgctgtat ctgatgcgca gcgaattgcg agagatcccc ccacacgact
    21541 ggggtaagac gctgaaagag atggatttaa atctctcttt cgatctgcgt gtcgagccac
    21601 tgagtaaata ccatcttgat gatatttcca tgcaccgact gcgtggcggc gaaattgtcg
    21661 ccctggacga tcagtacacg tttttgcagc gtatcccgcg cagccactac gtgctggcag
    21721 ttggtcctgt tccttatctt tattacctcc atcaaatgcg attgctggat atcgccctga
    21781 tcgcttttat tgctatttcc ctcgcctttc cggtgtttat ctggatgcgt ccgcactggc
    21841 aggatatgtt aaaactggaa gcagcggcgc aacgatttgg cgatgggcat ctcaatgaac
    21901 gtatccactt tgatgagggt tcgagctttg aacgacttgg tatcgcattt aaccagatgg
    21961 cggacaatat caacgcctta attgccagca aaaaacagct tattgacggt atcgctcacg
    22021 aactgcgaac accgttagtg cgcctgcgtt atcgactgga gatgagcgat aacctgagcg
    22081 ccgccgaatc ccaggcgttg aatcgtgata tcagtcaact tgaagcttta attgaagagc
    22141 tgctgactta tgcccgactc gatcgcccac aaaacgagct tcatcttagc gaa
//
LOCUS       DADLTY010000061        21521 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.061, whole genome shotgun sequence.
ACCESSION   DADLTY010000061 DADLTY010000000
VERSION     DADLTY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.061"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..1544
                     /locus_tag="JN334_001923"
     CDS             <1..1544
                     /locus_tag="JN334_001923"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HAY7438076.1"
                     /translation="TSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNN
                     VRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQ
                     GKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKP
                     AAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQN
                     GQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRN
                     NLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGP
                     GARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLL
                     HRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPL
                     ADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTA
                     AILMV"
     gene            1703..2262
                     /locus_tag="JN334_001924"
                     /pseudo
     CDS             1703..2262
                     /locus_tag="JN334_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            2263..3912
                     /locus_tag="JN334_001925"
     CDS             2263..3912
                     /locus_tag="JN334_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HAY7438077.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3917..4693
                     /locus_tag="JN334_001926"
     CDS             3917..4693
                     /locus_tag="JN334_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HAY7438078.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4968..7817
                     /gene="rcsC"
                     /locus_tag="JN334_001927"
     CDS             4968..7817
                     /gene="rcsC"
                     /locus_tag="JN334_001927"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HAY7438079.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8017..8667)
                     /gene="rcsB"
                     /locus_tag="JN334_001928"
     CDS             complement(8017..8667)
                     /gene="rcsB"
                     /locus_tag="JN334_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HAY7438080.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8684..11356)
                     /gene="rcsD"
                     /locus_tag="JN334_001929"
     CDS             complement(8684..11356)
                     /gene="rcsD"
                     /locus_tag="JN334_001929"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HAY7438081.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            12094..13215
                     /gene="ompC"
                     /locus_tag="JN334_001930"
     CDS             12094..13215
                     /gene="ompC"
                     /locus_tag="JN334_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY7438082.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            13327..14382
                     /gene="apbE"
                     /locus_tag="JN334_001931"
     CDS             13327..14382
                     /gene="apbE"
                     /locus_tag="JN334_001931"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HAY7438083.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            14456..15520
                     /gene="ada"
                     /locus_tag="JN334_001932"
     CDS             14456..15520
                     /gene="ada"
                     /locus_tag="JN334_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HAY7438084.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            15520..16170
                     /gene="alkB"
                     /locus_tag="JN334_001933"
     CDS             15520..16170
                     /gene="alkB"
                     /locus_tag="JN334_001933"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HAY7438085.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            16246..17889
                     /gene="yojI"
                     /locus_tag="JN334_001934"
     CDS             16246..17889
                     /gene="yojI"
                     /locus_tag="JN334_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HAY7438086.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            18107..18466
                     /locus_tag="JN334_001935"
                     /pseudo
     CDS             18107..18466
                     /locus_tag="JN334_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            18700..19731
                     /locus_tag="JN334_001936"
     CDS             18700..19731
                     /locus_tag="JN334_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HAY7438087.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(19853..20341)
                     /gene="eco"
                     /locus_tag="JN334_001937"
     CDS             complement(19853..20341)
                     /gene="eco"
                     /locus_tag="JN334_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HAY7438088.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(20476..20640)
                     /locus_tag="JN334_001938"
     CDS             complement(20476..20640)
                     /locus_tag="JN334_001938"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438089.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            20749..21243
                     /gene="napF"
                     /locus_tag="JN334_001939"
     CDS             20749..21243
                     /gene="napF"
                     /locus_tag="JN334_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HAY7438090.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            21233..21496
                     /gene="napD"
                     /locus_tag="JN334_001940"
     CDS             21233..21496
                     /gene="napD"
                     /locus_tag="JN334_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HAY7438091.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
BASE COUNT         5213 a         5245 c         5768 g         5295 t
ORIGIN      
        1 taacctcgtc gctgaaaggt aaacctgttt ctgcgcagct aacggtaggc gtggtcgatg
       61 aaatgatcta cgcgctgcaa ccagaaatcg cgccgaatat cggcaaattt ttctatccgc
      121 tggggcgtaa caatgtgcgt accagctcca gtctgtcgtt tatcagctat gaccaggcgc
      181 tctccagcga gccggttgcg cctggcgcga ctaaccgcag cgagcggcga gtaaaaatgc
      241 ttgaacgtcc acggcgtgaa gaggtggata ccgcggcatg gatgccgtca ctcacaaccg
      301 ataaacaagg caaagcgtat ttcacgttcc tgatgcctga ttcgttaacc cgctggcgta
      361 tcaccgcgcg tgggatgaac ggcgacgggc tggtcgggca ggggcgtgct tatctgcgtt
      421 cggaaaaaaa tctctacatg aagtggagta tgccaacggt gtatcgcgtg ggcgacaaac
      481 cggcggcagg actgtttatc ttcagtcagc aggataacga accggtggcg ctggtgacta
      541 aatttgcagg cgctgagatg cgccagacgc tgacgctgca caaaggggcg aattatattt
      601 cgctgacgca gaatattcag caatctggct tgttaagtgc agaactgcaa caaaatgggc
      661 aagtgcagga cagcattagc acaaaactgt cttttgtgga caacagctgg cccgttgaac
      721 agcagaaaaa tgtcatgctc ggcggtggcg agaacgcgct gatgttgccc gagcaggcga
      781 gtaatatccg gctacaaagt agtgaaacgc cgcaggagat tttccgcaac aatcttgatg
      841 cgttagtcga tgaaccgtgg ggtggggtga tcaacaccgg tagccgtctg atcccgctca
      901 gtctcgcctg gcgttcgctt gccgatcatc aaagtgccgc cgctaacgac attcgtcaga
      961 tgattcagga taaccgtctg cggctgatgc aactggcggg gcccggagcg cgctttacct
     1021 ggtggggtga agatggcaat ggtgacgcct tccttacggc atgggcatgg tacgccgact
     1081 ggcaggccag ccaggcgctc ggcgtaacgc aacaaccgga atactggcag catatgctcg
     1141 acagttatgc cgagcaggcg gataatatgc cgttattgca tcgggcgctg gtgctggcgt
     1201 gggcacagga gatgaatctg ccgtgcaaaa cgttgttgaa agggggggat gaagctatcg
     1261 cccggcgcgg aactaaagat gaagatttct ctgaggaaga catccgcgat atcaatgaca
     1321 gcctgatcct cgatacaccg gaatctccac tggcagatgc ggtggcaaac gtcttaacca
     1381 tgacgttgct gaaaaaagcg cagttgaagt ccacggtgat gccacaggtt cagcaatatg
     1441 cgtgggataa agcggcaaac agcaatcagc cgctggcgca cacggttgtg ctgctcaata
     1501 gcggtggtga cgctacccag acggccgcta ttttaatggt ttgaccgctg agcaatccac
     1561 tattgagcgc gcgctggcca tgaactggct ggcgaaatat atggcgacaa tgcctccagt
     1621 tgttttgcct gcgcctgcgg gcgcatgggc taaacataag ttaactggag ggggcgaaga
     1681 ctggcgttgg gttggttagg gtgtgccgga cattctctct tttggtgacg aattatcgcc
     1741 gcaaatgtgc aggtccgctg gcgtgaaccg gcaaaaacgg ctcaacaaag taacattccg
     1801 gtgaccgttg aacgccagtt gtatcggctt atccccggtg aagaagagat gagctttact
     1861 ctgcagccgg tgaccagcaa tgagattgac agcgatgcgc tgtatctcga tgaaattacg
     1921 cttaccagcg agcaggatgc agttctgcgc tatggtcagg tggaagtacc gctcccgccg
     1981 ggagctgacg ttgagcgcac aacatggggc atttcagtca ataaacccaa cgccgcgaaa
     2041 cagcaggggc aattgctgga aaaagcgcgt aatgaaatgg gcgaactggc ctatatggtg
     2101 ccggtgaaag aactgacggg aacggtcact ttccgccatt tgctgcgctt ctcgcaaaaa
     2161 gggcaattcg ttctgcctcc tgctcgttat gtgcgttcct atgcacctgc gcagcaaagt
     2221 gtcgcggcag ggagtgaatg gaccgggatg caggtgaaat aagtgaactg gcgcagaatc
     2281 gtctggctat tggcgttggt tactctgcca acgcttgcgg aggagacgcc gttacaactg
     2341 gtgctgcgcg gtgcgcagca cgatcaactg tatcagctgt cgtcatcagg cgtgaccaaa
     2401 gtctcagcat tacctgactc gctcaccacg ccgttaggga gtttgtggaa actgtatgtt
     2461 tacgcctggc tggaggatac ccatcaaccg gagcagccct accagtgtcg cggaaactcc
     2521 ccggaagagg tttattgttg tcaggctggg gagagcataa cgcgcgatac cgcgctggta
     2581 cgttcctgtg ggctctattt tgcgccgcag cggttgcata ttggcgcaga tgtgtggggg
     2641 caatactggc aacagcgtca ggcacctgcg tggttagcgt ctcttacaac gctgaagcca
     2701 gaaacctcgg taacggttaa atcgctgctt gattcattag cgacgttacc tgcgcaaaac
     2761 aaagctcagg aagtcttact ggatgtggtg ctggacgagg cgaaaatcgg tgtcgcatcc
     2821 atgctcggta gtcgggtacg ggtgaaaacc tggagctggt ttgccgatga taaacaggaa
     2881 atccgtcagg gtggttttgc tggctggtta accgatggta caccgctgtg ggttactggc
     2941 agtggcacca gcaaaacggt actaacccgt tatgcgacgg tgcttaatcg ggttttgcca
     3001 gtgccgacgc aggttgccag tgggcaatgt gtagaggtgg agctgtttgc ccgctatccg
     3061 ctgaagaaaa tcacagcgga aaagagtacg acggcagtaa aacccagcgt acttaatggg
     3121 cgctatcgtg tgacgtttac taatggtaac cacattacgt ttgtcagcca cggtgagacg
     3181 accttactga gcgaaaaggg caaactgaaa ttgcagtcgc atctcgatcg tgaagagtac
     3241 gttgccagag ttctggatcg cgaggcgaaa agtacgccac ctgaggcagc gaaagccatg
     3301 actgtggcga tacgtacttt tttacagcaa aacgccaatc gggaaggtga ttgcctgacc
     3361 atccccgaca gcagcgccac gcagcgtgtt tctgcttcgc cagcgaccac gggggcgcga
     3421 acgatgaccg cctggacgca ggagctcatc tatgcaggcg atccggttca ttatcatggc
     3481 agtcgcgcta ccgaagggac actttcctgg cggcaagcca cggcccaggc ggggcagggg
     3541 gagcgttacg accagatact cgcgtttgct tatcccgaca acagccttag ccgctgggga
     3601 gctccacgct caacctgcca gttattaccc aaagcgaaag cctggctggc gaaaaaaatg
     3661 ccgcagtggc ggcgtatatt acaaggcgag acggggtaca acgaaccaga cgtgtttgcg
     3721 gtctgtcgtt tagtctcagg tttcccctat accgatcggc agcagaaacg gctgtttatt
     3781 cgtaacttct ttacgcttca ggatcggctg gatttaaccc atgagtatct gcacttggcc
     3841 tttgatggtt atcccaccgg gcttgatgag aactatatcg aaacgctgac ccgacaatta
     3901 ttaatggact aatgctatgc gaaagatttt tcttccgttg ttactggtgg cactttcgcc
     3961 tgtcgctcac agtgaaggtg tgcaggaagt cgagattgac gcaccgctct ctggctggca
     4021 tccggcagag ggcgaagatg ccagtttttc acaatcgatc aattacccgg cgtcgtccgt
     4081 caacatggca gatgatcaaa atatctcagc gcagatccgc ggcaaaataa agaattatgc
     4141 tgcggcgggt aaagttcagc agggccggct ggtggtcaac ggtgccagta tgccgcagag
     4201 aattgaatcc gatggttcat ttgcacgccc ttatattttc actgaaggca gcaacagcgt
     4261 gcaggtcatc agcccggatg ggcaaagccg gcaaaaaatg cagttttact caacgccggg
     4321 tacaggaact attcgtgcac gtttacggct ggttctctcg tgggatacgg acaataccga
     4381 tctcgatctc catgttgtca cgcccgatgg cgaacacgcc tggtacggta acaccgtgct
     4441 gaaaaacagt ggtgcactgg atatggatgt cacgacgggg tacggacccg agattttcgc
     4501 catgccagca ccagttcacg ggcgctatca ggtgtatatc aactactatg gtgggcgcag
     4561 cgaaacggaa ttgacgaccg cccaactgac gctgatcacc gatgaaggat cggtcaatga
     4621 gaaacaggaa acatttattg taccgatgcg taatgctggc gagctgacgc tggtgaaaag
     4681 ttttgactgg tgatccatcc cttcacacaa cctggctctc tcttttttca gataagactg
     4741 ctgggcgatc tgccgacgcc aggatataac cattaacaag cgtcggcaga ttggtcattt
     4801 cttatttttc gtactgagaa ctgtccatct actccggctt tcctggttaa ggcaatggtt
     4861 tctaagcgat acgcattatt gcaatggtga cagaacattg cggtaacacg cttttaccgc
     4921 taccttaacc acactccatc ggtcacctga ggcggagctt ctcccctttg aaataccttg
     4981 cttcttttcg tacaaccctg aaagcctcgc gctacatgtt cagagcattg gcgttagtgc
     5041 tctggctgtt gattgctttt tcatccgttt tttacatcgt taatgcgtta catcagcgag
     5101 aatcggaaat tcgtcaggaa tttaatctga gttccgatca ggctcagcgc tttattcaac
     5161 gcacctctga tgtgatgaaa gagcttaagt acatcgccga aaatcgctta tcggcagaaa
     5221 acggtgtgct ttccccgcgt ggacgagaaa cgcaggcgga tgtgcctgcg tttgaaccgc
     5281 tgtttgccga ctccgattgt tccgcaatga gtaacacctg gcgaggttct ctggagtcat
     5341 tggcgtggtt tatgcgctac tggcgcgata atttttctgc ggcttacgat ctcaaccggg
     5401 tatttttaat cggcagtgat aacctctgca tggccaattt cggtctgcgt gatatgccag
     5461 tggaacgcga taccgcgttg aaagcgttgc atgaacgcat caataaatat cgaaatgcac
     5521 cacaagatga tagcggcagt aacctctact ggatcagcga aggtccgcgc ccttgcgtcg
     5581 ggtattttta cgcgttgacg ccagtttatc tggcgaaccg gttgcaggcg cttttgggtg
     5641 tcgagcagac catccggatg gagaactttt tcttaccggg tacgttgccg atgggggtta
     5701 ccattcttga tgaaaatggt cataccctga tttcgcttac cggaccagaa agtaaaatta
     5761 agggcgatcc tcgctggatg caggaacgct cctggtttgg ctatacggaa gggttccggg
     5821 agctggtgct gaagaaaaat ctgccaccct catcgctaag catcgtgtat tcggtgccgg
     5881 ttgataaggt gctggaacgc attcgcatgt tgatccttaa cgcaattttg ctgaatgtgc
     5941 ttgccggagc tgcattgttt actctcgcgc ggatgtacga gcgacgtatt ttcattccgg
     6001 cggaaagcga cgccctgcga ctggaagaac atgagcagtt caatcgcaag attgtcgcct
     6061 ccgcgccagt gggtatctgc attttgcgta ccgctgatgg cgtcaatatt ttaagtaacg
     6121 aactggcgca tacctatctc aatatgctta cgcatgagga ccgccaacga ctgacgcaaa
     6181 ttatctgtgg gcagcaggtc aattttgttg atgtcctgac cagcaacaat accaatctgc
     6241 aaatcagctt cgtccattcg cgctatcgta atgaaaacgt ggccatttgt gtgctggtgg
     6301 atgtttcttc gcgcgtgaag atggaagagt cgttgcagga gatggcacaa gcagcggaac
     6361 aggcgagcca gtcaaaatcg atgttccttg ccaccgtcag tcatgagctg cgaacgccgc
     6421 tgtatggcat tatcggtaac ctggatctgt tgcaaaccaa agagttaccg aaaggcgtcg
     6481 atcgtctggt gacggcaatg aacaactctt ccagcctgtt gttgaaaatt atcagcgata
     6541 ttctcgattt ctcgaagatt gaatcggaac agttgaagat cgaaccgcgt gagttttcac
     6601 cgcgtgaagt gatgaaccac atcaccgcca actatttacc gctggtggta cgcaagcagt
     6661 taggcttgta ctgctttatt gaaccggatg tgccagtggc cttaaatggc gacccgatgc
     6721 gtttacagca ggtcatctcc aacctgttga gtaacgccat aaaattcacc gataccggct
     6781 gtatagtttt gcatgttcgc gctgatggcg attatctctc tatccgtgtt cgcgataccg
     6841 gcgtggggat tccggcgaaa gaagtggtgc gcttgtttga tcccttcttc caggttggaa
     6901 cgggcgtaca gcgaaacttc caggggaccg gtctgggtct ggcgatttgt gaaaaactga
     6961 tcagcatgat ggacggcgat atctcggtag attcagaacc gggaatgggc agccagttta
     7021 ccgtgcgtat tccgttgtac ggcgctcagt acccgcagaa aaaaggcgtg gaagggttga
     7081 gtggtaaacg ctgctggctg gcggtccgca atgcgtcgct ctgtcagttc ctggaaacca
     7141 gtttgcagcg cagcggcatc gtcgttacaa catacgaagg gcaggaaccg actcccgaag
     7201 atgtgttaat cactgacgag gtagtgagta aaaaatggca gggcagagcg gtagtgacct
     7261 tctgtcgtcg ccatattggt attccgctgg agaaagcgcc aggggagtgg gtacacagtg
     7321 tggctgctcc gcatgagcta ccggcattgt tggcgcgtat ttatttgatc gagatggaga
     7381 gcgacgatcc tgctaacgct ctgccgtcga cggacaaagc ggtcagcgat aacgacgata
     7441 tgatgattct ggtcgtggat gatcatccga ttaaccgacg tctgctggca gatcagttgg
     7501 gatcgttggg ctatcaatgt aaaaccgcga atgatggcgt cgatgcgctt aatgtactta
     7561 gtaagaatca tattgatatc gtgcttagcg acgtcaacat gccaaatatg gatggttacc
     7621 gcttgacgca acgcattcgt cagttgggac tgacgttgcc ggtaatcgga gtaactgcta
     7681 atgcgttggc tgaagagaag cagcggtgtc tggagtccgg tatggacagc tgcctctcga
     7741 agccggtaac gctggatgtg ataaaacaga cgctgacggt atatgccgag agggtcagga
     7801 aatcgcggga ttcgtaggac ggataaggcg tttacgccgc atccggcatt cagtgcaaat
     7861 gccagataag acactgacgc gtcttatctg gcctacttta atgtaatctg ttgaaataat
     7921 gggaatcgta ggccggataa ggcgtttacg ccgcatccgg caatcggtgc aaatgccaga
     7981 taagacacta acgcgtctta tctggcctac agatgattag tctttatctg ccggacttaa
     8041 ggtcacagaa gagagataat tcagcagggc gatatcgttc tcgacgccca gcttcatcat
     8101 cgcagatttc ttctggctac tgatggtttt aatactgcgg ttcagctttt tagcgatctc
     8161 ggtcactagg aagccttccg caaacaggcg cagaacttca ctctcttttg gcgagagacg
     8221 cttgtcaccg taaccaccag cactgatttt ttccaacagg cgagaaacgc tttccggggt
     8281 aaatttcttc cctttctgca gcgcggcgag agctttcggc agatcggtcg gtgcaccttg
     8341 tttcagcacg atcccttcga tatccagatc caataccgca ctaagaatcg ccgggttgtt
     8401 gttcatagtc agaacaatga tcgacaggct tgggaaatgg cgcttgatgt acttgattaa
     8461 ggtaatgcca tcgccgtact tatcgccagg catggagaga tcggtaatca acacatgcgc
     8521 atccagtttc ggcaggttgt tgatcagtgc tgtagagtct tcaaattcgc cgacaacatt
     8581 cacccactca atttgctcaa gtgatttgcg aataccgaac aagactatcg gatggtcatc
     8641 ggcaataatt acgttcatat tgttcatgta ataggctacc ttgctacagc aagctcttga
     8701 cataactgtc aatgtcgctg atgtattttt ctattcctgg aacatccttc tcacgaatca
     8761 gatgttccag cgtttcacat aactgcttgc cgggtaccag atttagcatg gcaaatacgc
     8821 ctttaagacg atgagccgtt tgggctaacg cagcaaagtc actggttgct gcttcagtat
     8881 acagcctctt aacatcatcc ggtactgtgt ctacaaagag cgcataatag ccgctggcat
     8941 ggagttgcgc attttcatct ccgcccagag gcgattctgt cacctcttcc tgtgccagtt
     9001 gcacttcaat taattgtaag accgcttcct gcatagcgtt gctcatattg aagttgacgc
     9061 acaattgacc aggcccaatt tctcgtacgc cagactcatc atcgcttaaa agcaagccag
     9121 aggcagtaag attagacgga ttatccgtta aaaagatatc ataatcttga ctaattaatc
     9181 tttcatcggg tgtgatacag gttgcacccc aattttctaa caggcgagtg acaatattcc
     9241 gaatttctgc cgaagtaaca tccaccatta cgcagacatc atccagtaaa cgctcttctt
     9301 cctcttcaac ttccgggtca gcggcgagca ttttgatatg cacagagtag cgtgtaccaa
     9361 gcccatcccg cgttttgatg tttaaatgac cgcccagttt acgtgccagt tgatcgctca
     9421 gccagaatgc cagcgggtcc gcctttccat aacgatcgtt ttgggtctgg ttgataaacg
     9481 ggaagtgcaa attatccatt tcatgaatac ttacgccttc tcccgtgtcc agaatgcgga
     9541 atgtcaggcg gtcttcagag gactcatcct gatcaacctc aagggtgatt tttcccaatt
     9601 gcgttgaggt cacggcatat tgcatcagta gcagcaaaat acgtcgtaag gcatcgcgat
     9661 cgccgcggcg catatcgtgt gctttcagat gattgttaat cagcagttgc agacctttac
     9721 gcttgatggc aggcaacact gaaggcacaa cttcatcaat taaatcctgc acggagaaca
     9781 gcaccgtctc acttttccag ctatcatccg caagcatgtt cgctaactgt atttcttcga
     9841 ccagccgcac cagcacatct gcctgattcg ccagttgttt gctttccggg gcgttgagtt
     9901 tagccgcact ctccgccagg gactgtgcgg gttctttcag cgcatcgcca atgtttttca
     9961 taaaggtcat ccgcccctgc tggtttttct catacagacg ctgcgcctgc ttgagtttct
    10021 tgtttaccag cacttcgcga tcctgatcgc gaataatgaa aatttgtgtg cgcggcgcga
    10081 cctggctgcg gaacatgcgg atctcataca gctcgttatt gatcgtcgcc tgaataatcc
    10141 cctgatgctg ttccgccatg gtggtgatgt tttgcagatt cagatgcggc agcaaatgat
    10201 cggcaatttt gttacttatg acagtgcggt tcgattcctg atcgtgaacc agcaggccga
    10261 gcggcaacag tgagactatc tcttcattga ttgcccgtaa aatgcgcaat tcgttattga
    10321 ccgccgtgct ggggacgctt tctgtactgc ggctggagaa atggcggaat gtggtatagc
    10381 caaataacgc cagcgccagc aaaccgatgt tcagcagcag tggcagcaga atgttttgca
    10441 acgtatccag caataaggtg ccataaggaa cctgccagac caggcgcata tcggtagagt
    10501 tgagtgccga ggagatttca atcttcgtac tgttaaagtg gatactgacg ctatccgtcc
    10561 cttctttctc attatcattg tttcccgttg ccgtcgcgtc tggctcaagg cggaaactgt
    10621 ccagcggcat acccggtggg atcaaatcat taatcggcag atcaaaagcc acgaccgttg
    10681 ccagatgtcc cggctggttg aacgtggtac gcaaggtaaa gtaatgaccg ttctgccagg
    10741 ctaggcggcg catgttagaa aaactttcgc gttcatcgag ggcgttggcc tgttgtaaca
    10801 tttctgcacg acgtgaatca acaatgtcac tgacggtcga ttctttaaat ccggaggtga
    10861 gatctttaag gggtagggtt gagatcagca ccagactgtt atcctgacca ttcaggtaat
    10921 acatcgacca cggtacattt tctgcacccc acaatgtatc cagataagtg gacatccgct
    10981 gagtcatctc aagcgttgag ttgtcgtgag agccaaagat taacgcttcc gttttgcggc
    11041 gtggtttctc cagatagtag acatcctgtt tcaggcgcgt ctcttgtaaa ccttcgccgg
    11101 aggaggggga ggtcgtcgcg gcaatgttgt cgtagatctg cctggtcacg taacgccagg
    11161 tatcgacgcg cttttggatt gcgtgggtaa tgtcgacaat ctggtaactt ttatctttca
    11221 gccaagcgtt aacggcactt tgtaccatta cccccatcgt caccagtaac acaatgatca
    11281 acagtaaaaa gaagcgggta atgctccccg gtaggagtga aaagcgggtc gtggccgttg
    11341 tctctttctg acgcattcgt gtttatgacc tgttaaaact tcgcgaagtg atagggcagt
    11401 ataaagggta cagtgtgaaa ttccagactc ttacctgatt gatgattctc tattctcccg
    11461 tgatatttat catcctgtat ggggggaaat agatgtacaa gcgccatttt gctgtacaaa
    11521 taacctacaa aaagcccaac gaaattaatt gcaaataaac aaagattgct ggaaattatg
    11581 ctgatgttaa ttatttgtga aatggttaac aagcgttata gtttttctgt ggtagcacag
    11641 aataatgaaa agtgtgtaaa gaagggtaaa aaaaccgaat gcgaggcatc cggttgaaat
    11701 aggggtaaac agacattcag aaatgaatga cggtaataaa taaagttaat gatgatagcg
    11761 ggagttattc tagttgcgag tgaaggtttt gttttgacat tcagtgctgt caaatactta
    11821 agaataagtt attgatttta accttgaatt attattgctt gatgttaggt gcttatttcg
    11881 ccattccgca ataatcttaa aaagttccct tgtatttaca ttttgaaaca tctatagcga
    11941 taaatgaaac atcttaaaag ttttagtatc atattcgtgt tggattattc tgcatttttg
    12001 gggagaatgg acttgccgac tgattaatga gggttaatca gtatgcagtg gcataaaaaa
    12061 gcaaataaag gcatataaca gagggttaat aacatgaaag ttaaagtact gtccctcctg
    12121 gtcccagctc tgctggtagc aggcgcagca aacgctgctg aagtttacaa caaagacggc
    12181 aacaaattag atctgtacgg taaagtagac ggcctgcact atttctctga cgacaagtct
    12241 gtagatggcg accagaccta catgcgtctt ggcttcaaag gtgaaactca ggttactgac
    12301 cagctgaccg gttacggcca gtgggaatat cagatccagg gcaacagcgc tgaaaacgaa
    12361 aacaactcct ggacccgtgt ggcattcgca ggtctgaaat tccaggatgt aggttctttc
    12421 gactacggtc gtaactacgg cgtagtttac gacgtaactt cctggaccga cgtactgcca
    12481 gaattcggtg gtgacactta cggttctgac aacttcatgc agcagcgtgg taacggcttc
    12541 gcaacctacc gtagcaccga cttctttggt ctggttgacg gtctgaactt cgctgttcag
    12601 tatcagggca aaaacggtag cccggaaggt gaaggcatga ccaacaacgg tcgtgaagct
    12661 ctgcgtcaga acggtgacgg cgttggcggt tctattactt atgattacga aggcttcggt
    12721 atcggtgctg cagtttccag ctccaaacgt actgatgacc agaacttcgg tctgaaccgt
    12781 tatgatgagc gttacatcgg taatggcgac cgcgctgaaa cctacaccgg tggtctgaaa
    12841 tacgacgcga acaacatcta cctagctgct cagtacaccc agacctacaa cgcaactcgc
    12901 gtaggcaact tgggttgggc gaacaaagca cagaacttcg aagctgttgc tcagtaccag
    12961 ttcgacttcg gtctgcgtcc gtccctggct tacctgcagt ctaaaggtaa aaacctgggt
    13021 gtcatcaatg gtcgtaacta cgacgacgaa gatatcctga aatatgttga tgttggtgcg
    13081 acctactact tcaacaaaaa catgtccacc tacgttgact acaaaatcaa cctgctggac
    13141 gacaaccagt tcactcgtga cgctggcatc aacactgata acatcgtagc tctgggtctg
    13201 gtttaccagt tctaatctcg atggatatcg aacaaagggc ctgcgggccc ttttttcatt
    13261 gttttcagcg tacaaacaca gttttttggt gtactcttgc gaccgttcgc atgaggataa
    13321 tcacgtatgg aaataagctt tacccgcgtg gcactgctgg ttgccgcgct cttctttgtt
    13381 ggttgcgatc aaaaaccaca acccgccaaa acccacgcta ctgaagttac cgttcttgaa
    13441 ggcaaaacca tgggtacctt ctggcgtgcc agcatcccgg gcattgatgc caaacgcagt
    13501 gccgaactta aagaaaagat tcagacccag ctggacgccg acgatcagct gctttcgacc
    13561 tataaaaaag attccgcgct gatgcgcttt aacgactcgc aaagtttgtc gccgtggccg
    13621 gtaagtgaag cgatggccga tatcgtcacc acctcgctgc gcattggggc aaggaccgat
    13681 ggtgcgatgg atattaccgt cgggccgctg gtgaatctgt ggggctttgg cccggaacaa
    13741 cagccggttc aaattccgag ccaggaacag atcgatgcga tgaaagccaa aaccggctta
    13801 cagcacctga cggtcatcaa tcagtcgcat cagcaatatc tgcaaaaaga cctgccggat
    13861 ttatatgtcg atctctccac cgtcggtgaa ggttatgcgg cggatcacct ggcacgcttg
    13921 atggagcagg aagggatttc ccgctatctg gtgtcggtgg gcggcgcgct gaacagccgt
    13981 ggtatgaacg gtgaaggcca gccgtggcgg gtagcgattc aaaaaccaac cgataaagaa
    14041 aacgcggttc aggctgtggt ggatatcaac ggtcatggga ttagcacctc tggcagctac
    14101 cgtaactatt acgaactgga cggcaaacgt ctttcccatg ttatcgatcc gcaaaccggg
    14161 cgtcccatcg aacacaatct ggtatccgtg acggtgattg ctccgacggc gctggaagcc
    14221 gatgcctggg atactggctt gatggtactc gggccggaga aagccaaaga ggttgttcgc
    14281 cgggaagggc tggcggttta tatgatcacc aaagaaggcg atagctttaa aacctggatg
    14341 tcaccacagt ttaaaagctt ccttgtcagc gaaaaaaatt aaagcgcaag attgttggtt
    14401 tttgcgtgat ggtgaccggg cagactaaag gctatcctta accagggagc tgattatgaa
    14461 aaacgccaca tgcttaactg acgatcaacg ctggcaatct gtcttagccc gcgacccgaa
    14521 tgccgacggc gaattcgttt tcgccgtgcg caccacgggt atcttttgcc gtccttcttg
    14581 ccgcgccaga catgccttgc gggaaaatgt ctccttctac gcaaatgcca gcgaggcact
    14641 cgccgccgga tttcgcccct gcaaacgttg tcagccagac aaagccaatc cccggcaaca
    14701 tcggttggat aaaatcaccc acgcgtgtcg actgctggaa caggaaacgc ctgtaacgct
    14761 ggaagcctta gccgaccagg tggcgatgag tccatttcat ctacatcggt tgtttaaagc
    14821 tactaccgga atgacgccta aagcctggca acaggcctgg cgcgctcgcc gtttgcgcga
    14881 atcgctggcg aaaggggaga gcgtgacgac gtctattctt aacgccggat tccccgacag
    14941 cagcagttac taccgcaaag ccgatgaaac gctgggcatg acggctaaac aattccgtca
    15001 cggtggcgaa aatctggcgg tgcgttacgc gctggctgat tgtgagctgg gtcgttgcct
    15061 ggtggcagaa agcgagcggg ggatttgcgc gatattgctg ggcgatgatg acgcgacatt
    15121 aatcagcgag ttgcagcaga tgtttcccgc tgccgacaac gcgcctgccg atctgacgtt
    15181 tcagcaacat gtgcgtgaag tgatcgccag cctcaatcaa cgcgatacgc cgctgacgtt
    15241 accgctggat attcgcggca ctgcttttca gcaacaagtc tggcaggcac tgcgcacgat
    15301 accttgtggt gaaaccgtca gttatcagca actggctaac gccatcggca aaccgaaagc
    15361 ggtacgggcc gttgccagcg cctgtgccgc caacaagctg gctatcgtaa taccctgtca
    15421 tcgggtggtc cgtggtgatg gcacactttc cggttaccgc tggggcgtgt cgcgtaaagc
    15481 gcaactgctg cgccgcgaag ctgaaaatga ggagaggtaa tgttggatct gtttgccgat
    15541 gctgaaccgt ggcaagagcc actggcggct ggtgcggtaa ttttacggcg ttttgctttt
    15601 aatgctgcgg agcaactaat ccgcgatatt aatgacgttg ccagccagtc gccgtttcgc
    15661 cagatggtcg ctccaggcgg atataccatg tcggtggcga tgaccaactg tgggcatctg
    15721 ggctggacga cccatcggca aggttatctc tattcgccca ttgatccgca aacaaataaa
    15781 ccgtggcccg ctatgccaca aagctttcat gatttatgtc aacgtgcggc tacggcggcg
    15841 ggctatccag atttccagcc agatgcttgt cttatcaacc gctacgctcc tggcgcgaaa
    15901 ctgtcgctgc atcaggataa agacgaaccg gatctgcgcg cgccaattgt ttctgtttct
    15961 ctgggcttac ccgcgatttt tcaatttggt ggcctgaaac gtaatgatcc gctcaaacgt
    16021 ttgttgttgg aacatggcga tgtggtggta tggggcggtg aatcgcggct gttttatcac
    16081 ggtattcaac cgttgaaagc ggggtttcat ccactcacca ccgactgccg ctacaacctg
    16141 acattccgtc aggcaggtaa aaaagaataa aaataagaat tattattgct gtgcgcgcga
    16201 agatgtttaa actgcgggct gtaattcatt gtccgggttt tctgcatgga acttcttgta
    16261 cttgtctggc ggcagtatcg ctggccattt atcagtgtga tggcgctaag cctcgccagt
    16321 gcggcattag gcattggctt aattgctttt atcaatcagc gccttatcga aacggcggat
    16381 accagtctgc tggtgttacc ggagtttctg ggattattgc tgctgttgat ggcagtcact
    16441 ctcggatcgc aactggcgct caccactttg gggcatcact tcgtttaccg actgcgtagt
    16501 gaatttatca agcggattct ggatacccac gtcgagcgca ttgaacaact cggtagcgcc
    16561 tcgttgctgg cgggattaac cagcgatgtg cgcaatatca ccattgcttt tgtgcgtctg
    16621 ccggaactgg ggcaggggat cattctcact atcggttcat cggcgtatct gtggatgctg
    16681 tcgggcaaaa tgttgctggt aacggctatc tggatggcga tcaccatctg gggcggtttt
    16741 gtgctggtgg cgcgggtgta caaacatatg gcgaccctac gtgaaaccga agacaagctg
    16801 tacacggatt ttcaaactgt acttgaaggg cgcaaagagc tgactctgaa ccgggaacgc
    16861 gccgagtatg tgtttaacaa cctctacatc cccgatgcgc aagagtatcg ccatcatatt
    16921 attcgcgcag acaccttcca tcttagtgcc gtgaactggt caaacatcat gatgctgggc
    16981 gctatcggcc tggtgttctg gatggcgaac agtctcggtt gggctgatac caacgttgct
    17041 gcgacctatt cgttgacgct gttattcctg cgtacgccgc tgctttcggc ggttggcgca
    17101 ttgccgacgc tgctgacggc gcaggtggcg tttaacaagc tgaacaaatt cgcgctcgcg
    17161 cctttcaaag ctgagtttcc gcgcccgcag gcgtttccca actggcaaac gctggagctg
    17221 cgtaacgtga cgtttgctta tcaggataac gcgttttccg ttggtccgat taatctcacc
    17281 atcaaacgtg gcgagctgct gtttctgatt ggcggtaacg gtagcggaaa atcgacgctg
    17341 gcgatgttgt tgacgggctt gtatcagcca caaagcggcg aaatcttgct ggatggcaaa
    17401 cctgtcagcg gcgaacaacc ggaagattat cgcaaactgt tttcggcagt gtttaccgat
    17461 gtctggctgt ttgatcaact gctggggccg gaggggcaac ccgctaaccc gcaactggtt
    17521 gagaagtggc tggcgcagct gaaaatggct cataagcttg agttaagcaa cgggcgtatt
    17581 gttaacctga agttatcaaa agggcagaaa aaacgcgtgg cgctgttgct ggcgctggca
    17641 gaagaacgcg atattatcct gctggatgaa tgggcggtgg atcaggatcc acacttccgt
    17701 cgtgagtttt atcaggtgtt gctgccgctg atgcaggaga tgggtaaaac tattttcgct
    17761 atcagtcatg atgatcatta ctttatccac gccgaccgcc tgctggaaat gcgcaatggg
    17821 caacttagcg agctgacggg cgaagagcgc gatgccgctt cgcgtgatgc cgttgcccgg
    17881 acggcataat attacgacgc tgacctggcg gactgctgcc gtcaggtcaa tatgctaaaa
    17941 atcccttcat ttctaataac cctataatta atcaacgaaa ttataatgtt tctaaaatta
    18001 gaatataatt tataaacatt atttaaatgt tgttacttaa gtgttaccgt tgatgccgcg
    18061 caaatctcac ctgcaataaa gcgactaaaa gtaaggcatt aacaagatga aaaaagtgac
    18121 tgccatgctc ttctcgatgg ccgtggggct taatgccgtt tcgatggcgg caaaagcgaa
    18181 agcgtccgag gagcaggaaa ctgatgtact gttgattggc ggcggcatta tgagcgccac
    18241 gttggggacc tattgacgcg agctggagcc tgaatggtcg atgaccatgg tggagcgcct
    18301 ggaggggggc gcgcaggaga gttcgaacgg ctagaacaac gccggaaccg ggcattctgc
    18361 actgatggaa ctgaactaca ccccgcaaaa cgccgatggc agcatcagta ttgaaaaagc
    18421 agtcgccatt aacgaagcat ttcagatttc ccgccagttc tgggcgcacc aggtcgagcg
    18481 cggcgtgctg cgtactccgc gttcatttat caataccgtt ccgcatatga gctttgtctg
    18541 gggcgaggat aacgttaatt tcctgcgcgc ccgttacgcc gcgttgcaac aaagctcgct
    18601 gtttcgcggt atgcgttact ctgaagatca cgcgcagatc aaagagtggg caccgttagt
    18661 gatggaaggg cgcgatccgc aacagaaatt ggcactgatg tgaactatgg tgagatcacc
    18721 cgccagttaa ttgcttcctt acagaagaaa tctaacttct cgctgcaact cagcagcgaa
    18781 gtccgcgccc taaagcgtaa tgacgataac acctggaccg ttaccgttgc cgatctgaaa
    18841 aacggcactg cacagaacat ccgcgccaaa tttgtcttta tcggcgcggg cggtgcggcg
    18901 ctgaagctgt tacaggaatc ggggattccg gaagcgaaag actacgccgg tttcccggtg
    18961 ggcggacagt tccttgtttc ggaaaatcca gacgtggtta atcgccatct ggcgaaagtt
    19021 tacggtaaag catccgttgg cgcaccaccg atgtcggttc cgcatatcga tacccgtgtt
    19081 ctggacggta aacgcgtagt gctgtttggg ccatttgcca ccttctcaac caaattcctc
    19141 aaaaacggtt cattgtggga tctgatgagt tccaccacca cctctaacgt gatgccgatg
    19201 atgcacgtcg ggctggataa tttcgatctg gtgaaatatc tggtgagtca ggtgatgttg
    19261 agtgaagagg atcgttttga agcgttgaaa gagtactatc cgcaagcgaa aaaagaggac
    19321 tggcgtttgt ggcaagcggg gcagcgtgtg cagattatca agcgtgatgc cgagaaaggt
    19381 ggcgtactgc gtctgggtac cgaagtggtc agtgaccagc aagggaccat tgcggcgctg
    19441 ctgggggcat cgccaggggc gtcaaccgcc gcgccgatta tgttgaatct actggaaaaa
    19501 gtatttggcg atcgcgtttc cagcccgcaa tggcaggcta cgttgaaagc gatcgttccg
    19561 tcttatggac gcaagctgaa cggtgatgta gcggcaacag aacgcgagtt gcagtacacc
    19621 agcgaagtgc tggggctgaa atacgacaaa ccgcaagcag cagatagtac gccgaaaccg
    19681 cagttgaaac cgcaacccgt tcaaaaagaa gtggcggata ttgcgttgta atgatgtggc
    19741 gtatactgat aagacgcgcc agcgtcgcat caggcaaccg gctcgggcgt tagatgttgg
    19801 atgcggcgta accgccttat ccaaccaacg gggtgccgta cttcacaaca gtttagcgaa
    19861 cttccgcgtt gtcaattttc tcttccgcct tccagacgcg gtatttcaca tctacattgt
    19921 ctggcgtata caccacgatc ggcagcttgc tgttgtaacg cagcattcca gcatcgccca
    19981 gatacgcggt gacaaatttc ttctctttct tgccatccgg gcaggccatc atcgtggaaa
    20041 ccggcgagct aactttatca aagacataat aatcatagcc ccagccttcc agcgttttgc
    20101 tttccagctt cccgccgaga cgatgcaaat tgcaatcgac ttccagcgtc tgaccgatta
    20161 acagttctac tttcagggta gattcatctt cttgcggggt taactgaatc acctgacgct
    20221 tcatgcctgt ttcagcttgt ggataaggcg cgattttttc cagtggctgg acgctttctg
    20281 ccgcccaggc ggaagtggta gcgaaagcgg caaacaatac ttcaggtaga atggtcttca
    20341 tttttttgct ttccttcttc atttataggt tttcaccgat gatgtcgctg aaggttaaca
    20401 tattcatcag atttattaat ttgctttatg aataacttaa agaacattca gacaatttta
    20461 cctgctcttt tagtgttaaa ttctgatgag ggagcaaacc agcagaaata tctctggtga
    20521 attaatcgct atataaatat gtttataacc atttgaaatg tgagcaaaag cccgtttttg
    20581 ccacgcttcc cgccactctt ttgatcctgc tacaggtttt accccgatcg gggtatgcat
    20641 ctttgacaca tcctttaata tcttagcggc tataaaaatg gcttattaat tatgcggtct
    20701 atttggtcgc tctcaatttt cagagcgcgt taatgatgga aggtcaatgt gaagattgat
    20761 gcatcccgtc ggggcatact cactggttgc tggcgcaaag ccagtaacgg tatccgtccg
    20821 ccctggtccg gtgatgaatc tcattttctg acccattgca cccgctgcga cgcctgtatt
    20881 aatgcttgtg aaaacaacat tctgcaacgc ggcacgggtg gctatccgag cgttaatttc
    20941 aaaaataacg agtgcagctt ctgttacgcc tgcgcgcagg cttgccccga atcgctattt
    21001 tctccgcgcc acaccagggc ctgggatttg cagttcacca tcggggacgc gtgcctggcg
    21061 tatcagtcag ttgaatgccg ccgctgtcag gatagctgtg agccaatggc aattatcttt
    21121 cgcccgacgc tctccgggat ctaccaggcg caacttaata gccaactttg caacggatgt
    21181 ggagcatgtg cagccagctg tccggtatca gccataactg cggagtatct ccatgcacac
    21241 taactggcaa gtttgcagcc tggtcgtgca ggccaaaagc gaacgaattt cagacatcag
    21301 cacccaactg aacgcctttc ccggctgtga agttgctgtc agcgacgcgc cgagcggtca
    21361 gttgattgtg gtggtggaag cagaagacag cgaaacgctg atccaaacca ttgagtcagt
    21421 acgcaacgta gagggcgtgc tggcggtgtc gctggtttat caccagcagg aagagcaagg
    21481 tgaggaaaca ccatgaaact cagtcgtcgt agctttatga a
//
LOCUS       DADLTY010000062        21513 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.062, whole genome shotgun sequence.
ACCESSION   DADLTY010000062 DADLTY010000000
VERSION     DADLTY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.062"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(20..1018)
                     /gene="fbp"
                     /locus_tag="JN334_001941"
     CDS             complement(20..1018)
                     /gene="fbp"
                     /locus_tag="JN334_001941"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HAY7438092.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1194..2567
                     /gene="mpl"
                     /locus_tag="JN334_001942"
     CDS             1194..2567
                     /gene="mpl"
                     /locus_tag="JN334_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAY7438093.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2650..3201)
                     /locus_tag="JN334_001943"
     CDS             complement(2650..3201)
                     /locus_tag="JN334_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAY7438094.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3295..4647
                     /gene="pmbA"
                     /locus_tag="JN334_001944"
     CDS             3295..4647
                     /gene="pmbA"
                     /locus_tag="JN334_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAY7438095.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4830..5212
                     /gene="cybC"
                     /locus_tag="JN334_001945"
                     /pseudo
     CDS             4830..5212
                     /gene="cybC"
                     /locus_tag="JN334_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5257..5721)
                     /gene="nrdG"
                     /locus_tag="JN334_001946"
     CDS             complement(5257..5721)
                     /gene="nrdG"
                     /locus_tag="JN334_001946"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HAY7438096.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5879..8017)
                     /gene="nrdD"
                     /locus_tag="JN334_001947"
     CDS             complement(5879..8017)
                     /gene="nrdD"
                     /locus_tag="JN334_001947"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAY7438097.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8411..10066)
                     /gene="treC"
                     /locus_tag="JN334_001948"
     CDS             complement(8411..10066)
                     /gene="treC"
                     /locus_tag="JN334_001948"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAY7438098.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10116..11537)
                     /gene="treB"
                     /locus_tag="JN334_001949"
     CDS             complement(10116..11537)
                     /gene="treB"
                     /locus_tag="JN334_001949"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HAY7438099.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11656..12603)
                     /gene="treR"
                     /locus_tag="JN334_001950"
     CDS             complement(11656..12603)
                     /gene="treR"
                     /locus_tag="JN334_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HAY7438100.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12788..12841
                     /gene="mgtL"
                     /locus_tag="JN334_001951"
     CDS             12788..12841
                     /gene="mgtL"
                     /locus_tag="JN334_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HAY7438101.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12982..15678
                     /gene="mgtA"
                     /locus_tag="JN334_001952"
     CDS             12982..15678
                     /gene="mgtA"
                     /locus_tag="JN334_001952"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAY7438102.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15884..16270)
                     /gene="ridA"
                     /locus_tag="JN334_001953"
     CDS             complement(15884..16270)
                     /gene="ridA"
                     /locus_tag="JN334_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HAY7438103.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16343..16804)
                     /gene="pyrI"
                     /locus_tag="JN334_001954"
     CDS             complement(16343..16804)
                     /gene="pyrI"
                     /locus_tag="JN334_001954"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HAY7438104.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16817..17752)
                     /gene="pyrB"
                     /locus_tag="JN334_001955"
     CDS             complement(16817..17752)
                     /gene="pyrB"
                     /locus_tag="JN334_001955"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAY7438105.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17756..17890)
                     /gene="pyrL"
                     /locus_tag="JN334_001956"
     CDS             complement(17756..17890)
                     /gene="pyrL"
                     /locus_tag="JN334_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HAY7438106.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18171..18566)
                     /locus_tag="JN334_001957"
     CDS             complement(18171..18566)
                     /locus_tag="JN334_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY7438107.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18698..19411)
                     /gene="bdcA"
                     /locus_tag="JN334_001958"
     CDS             complement(18698..19411)
                     /gene="bdcA"
                     /locus_tag="JN334_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7438108.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19482..20075
                     /locus_tag="JN334_001959"
     CDS             19482..20075
                     /locus_tag="JN334_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7438109.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20220..20672
                     /locus_tag="JN334_001960"
     CDS             20220..20672
                     /locus_tag="JN334_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY7438110.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20795..>21513
                     /locus_tag="JN334_001961"
     CDS             20795..>21513
                     /locus_tag="JN334_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7438111.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKAL"
BASE COUNT         5196 a         5324 c         5668 g         5325 t
ORIGIN      
        1 tgtggagagg cgcaggagat tacgcgtccg ggaactcacg gataaagcgt tcgacatctt
       61 caaccatatg gtcgttgccg acaaagaacg aacggcgctg gtgcagagtt tccgggatga
      121 tatccagaat acgctctttg ccatcgctcg ctttaccgcc cgcttgttcc gccaggaatg
      181 ccatcgggtt gcactcataa agcaaacgca gtttgccgtc cgggtggctg gcggtgcttg
      241 ggtagagata aataccgcct ttcagcaggt tacggtggaa atccgcaacc agtgaaccga
      301 tataacgcga ggtatacggg cgattggtgg atttatcttc ttcctggcag aatttaatgt
      361 acttcttcac cccgttcgga aacttaatgt agtttccttc gttgatggag taggttttgc
      421 ctttctccgg gaagcgcatc cgttcctggc acaggcagaa aacgcccagc gaaggatcgt
      481 aagtaaaggc gtgaacaccg catccggtgg tgtaaaccag catggtagag gagccgtata
      541 ccacgtaacc tgccgcaacc tgtttgttac caggctggag gaaatcttct tccgttaccg
      601 gcgtgccaac aggcgtaacg cggcggtaga tggagaaaat ggtaccgaca gagacgttaa
      661 catcgatgtt ggacgagcca tccagggggt ccatcagcac cacgtatttt gcgtgttcac
      721 agccttcaaa gacgacaatc tcatcttctt cttcagaggc aatccccgca acgatatcgc
      781 gtgctttcag tgcggctttc agtttttcat tagcgaacaa gtcgagtttt tgctgaacct
      841 cgccctgcac gttctcagca ccgctggcac ccaggatatc aaccagtcct gctttgttga
      901 tatcgcgatg gataatcttg gcgcccagtt ttattgccga cagcaaagca gtgagctcac
      961 cggtagcatg agaaaactcg tgctgctttt cgacaataaa ttcacctaac gttttcataa
     1021 aactttccct gcaatgttta tggagtaaag cgaccgcaac aatcttaaca aataatctca
     1081 atgttgcgct caggtgaatc gcgccagcaa attgcggatt atcctgaaat gcgtttctca
     1141 cttgcccgac atatgcgtaa aatgagcggc agattaaaaa aggatagtga cgtatgcgca
     1201 ttcatatttt aggaatttgt ggcacattta tgggcggtct ggcgatgctg gcgcgccagt
     1261 taggccatga agtaacgggt tcggacgcca atgtgtatcc gccgatgagc accttacttg
     1321 agaagcaagg cattgagctg attcagggtt acgatgccag ccagctcgag ccgcagccgg
     1381 atctggtgat tattggcaac gccatgaccc gtggaaatcc gtgtgtggaa gcggtactgg
     1441 aaaaaaacat cccttatatg tcaggtccac agtggctgca cgattttgtg ctgcgcgacc
     1501 gctgggtgct ggccgttgcc ggtacacacg gcaaaaccac caccgcggga atggcgacct
     1561 ggattctgga acagtgtggt tacaaaccgg gatttgtgat cggcggtgtg ccggggaact
     1621 ttgaggtttc ggcgcgtctg ggcgaaagcg acttctttgt tatcgaagcg gatgagtacg
     1681 actgcgcctt cttcgacaaa cgctctaaat ttgtccatta ctgcccgcgt acgctgatcc
     1741 tcaacaacct tgagttcgat cacgccgata tctttgacga cctgaaagcg atccagaaac
     1801 agttccacca tctggtgcgt atcgttccgg ggcagggccg tattatctgg ccggaaaatg
     1861 acatcaacct gaaacagacc atggcgatgg gctgctggag cgagcaggag ctggtgggtg
     1921 agcagggtca ctggcaggcg aaaaagctga ccaccgatgc ttccgaatgg gaagttttgc
     1981 tggatggcga aaaagtgggc gaagtgaaat ggtcgctggt aggcgaacat aatatgcaca
     2041 atggcctgat ggcgattgca gcggctcgcc atgttggtgt agcgccggca gatgccgcta
     2101 acgcgctggg ttcgtttatt aatgctcgtc gccgtctgga gttgcgtggt gaagcgaatg
     2161 gcgtcacggt atatgacgat tttgcccatc acccgacggc gattctggca acgctggcgg
     2221 cgctgcgtgg caaagttggt ggtacggcgc gcattattgc tgtgctggaa ccgcgctcga
     2281 ataccatgaa aatggggatc tgcaaagacg atctggcacc ttcattaggt cgtgctgatg
     2341 aagtcttcct gctgcaaccg gcgcatattc cgtggcaggt ggcagaagtg gcagaagcct
     2401 gcgttcagcc tgcacactgg agtggcgatg tggatacgct ggcagatatg gtggtgaaaa
     2461 ccgctcagcc tggcgaccat attctggtga tgagcaacgg cggttttggt gggatccatc
     2521 agaaactgct ggatggtctg gcgaagaagg cggaagctgc gcagtaattc ggcttcagcc
     2581 tgatatagca ttgccggata aggcgtttac gccgcatccg gcaaaccata ctcacatcaa
     2641 caacgaaaat tacccttcat tctccgccag ttcgcgcaga tactggaaaa tctggcgtgc
     2701 ggatttcggc ggcttattcc cttctttctc tttcttcgcg ttgcggatca gtgtacgcag
     2761 ttgctgacga tcggcatcgg gccacagatt caatacttca gcgatggcgt catcaccttg
     2821 atcgatcaga cgatcgcgca ggttttccag tttatggaac agcaccacct gctggttgtg
     2881 acggtttttc agcttatcca gcgcctgacg aataggctct acgtcgcgct ggcgcagcat
     2941 cttaccgatg agctgcaact ggcggcggcg accttccatc ttaatacgct gggcgagctc
     3001 aatagccggg cgcagatccg catctaacgg gatcttatcc agcgcgtttt tccccagatc
     3061 aacaatttcc gcgccaaggc gttttagctc ctcggcatca cgtttaattt cacttttact
     3121 gacccagata atttcatcgt cttcgtcttc gatgtcatca ccgggaacgt cgtcgagcca
     3181 gtcttcgggc tgcttagtca tctcaggctc cttaaaaaaa gaggctaatg ttaccagtta
     3241 agatgcgcac tgaaaaacgg ttctctgtta gacttcagag aaactctcta cattatggca
     3301 cttgcaatga aagtaatctc tcaagttgaa gcgcagcgca agattctgga agaagcagtt
     3361 tccactgcgc tggagttggc ctcaggcaaa tcggacggtg cggaagttgc cgtcagcaag
     3421 accaccggca ttagcgtaag cacgcgttat ggtgaagtgg agaatgttga attcaatagt
     3481 gatggcgcgc tggggatcac tgtttatcac cagaaccgta aaggtagcgc atcgtctacc
     3541 gatttaagcc cgcaggccat tgcccgcacc gtacaggcgg cgctggatat tgcccgttat
     3601 acctcgccag atccctgtgc cggcgtggca gacaaagagc tgctggcctt tgacgcaccg
     3661 aatctcgact tgttccaccc tgcggaagtt tccccggatg aagccattga actggcggcc
     3721 cgcgcagaac aggcggcatt gcaggcggac aaacgcatca ccaataccga aggtggcagc
     3781 tttaacagcc actacggtgt caaagttttt ggcaacagcc acggcatgtt gcagggttac
     3841 tgctcaacgc gtcattcgct ctccagctgt gtaattgccg aagaaaatgg cgatatggag
     3901 cgtgattacg cctacaccat tggccgcgcg atgagcgatc tgcaaacgcc agagtgggtc
     3961 ggggccgact gtgctcgccg tactttatcg cgcctgtcac cgcgtaaact ctccaccatg
     4021 aaagcgccgg tcatttttgc caatgaagtg gcaaccgggc tttttggcca tctggtgggg
     4081 gcgatagcgg gtggctcggt ttaccgtaaa tctacctttc tgctggattc gctgggtaaa
     4141 caaattctgc cggactggct gaccattgaa gagcatccgc atctgctgaa agggctggcg
     4201 tcgacgccat tcgacagcga aggtgtgcgc accgagcgtc gcgatattat taaagatggc
     4261 atcctgactc agtggctgct gaccagctac tcggcgcgga aactggggct gaaaagcacc
     4321 ggacatgcgg gcggtattca caactggcgg attgccggac aagggctaag cttcgaacaa
     4381 atgctcaaag agatgggcac cgggctggtg gtgacggagc tgatgggcca gggcgtgagt
     4441 gccattaccg gtgattattc ccgtggtgca gcgggcttct gggtagagaa tggcgaaatt
     4501 cagtatcccg ttagtgaaat caccatcgca ggtaatttaa aagatatgtg gcgcaatatt
     4561 gtcaccgtcg gtaacgatat tgaaacacgc agtaatatac agtgtggttc tgtgctgttg
     4621 ccggagatga aaatcgccgg gcagtaaaaa actggcgcga cctgccgcgc tataaactaa
     4681 aaaattacac aaaatcattc gcactgcatc gagacggaaa gtgaatgagg gccggggagc
     4741 gtactcgcag tacgtgaccc gggcgaatga gcgtagccgt cgatgaggca gtgtgaagga
     4801 tgaagtgtaa ataaaaaagg aagtgagcaa tgcgtaaaag cctgttagct attcttgcag
     4861 tctcctcgtt ggtattcagt tctgcgtcgt ttgccgctga tcttgaagac aatatggaaa
     4921 ccctcaacga caatttaaaa gtgatcgaaa aagcggataa cgcggcgcaa gtcaaagacg
     4981 cgttaacgaa gatgcgcgcc gcagcgctgg atgcgcaaaa agcaacgccg ccgaagctcg
     5041 aagataaatc accggacagc ccggaaatga aagatttccg ccacggtttc gacattctgg
     5101 tcggtcagat tgacgacgcg ctgaagctgg caaatgaagg taaaaagaag cgcaggctgc
     5161 tgcagagcaa ctgaaaacga cccgcaacgc ctatcaccag aagtatcgtt aattcctcat
     5221 ttccctgttg cctgcactca ggtaacaggg aacttatcat cgcaaatgat gcaccacctg
     5281 attgctgctg ccgcgccaga tcagggaagg gtcttttaaa tcctgcacaa atttgccgtc
     5341 gaccagcacg ttaatcaaat caacaacctg catttgcgca gcgttgagtt cgtcgagttt
     5401 atagcctgtc cacacccaga tgtctttacc cggacactcg gcgcggatgc gttttaccag
     5461 tttcagaata tccggcacgt tttgcggatg cagcggatcg ccgccggaga gggaaatccc
     5521 ctggcgtttg atacgagtgt cattcagatc tttaatgatc ttgtcttcca ttgctttggt
     5581 aaatggctga ccagaattta cccgccatgt gcttttgtta tagcaaccgg ggcattcatg
     5641 aacacacccg gagacaaaca gggtgcagcg agtgccgggg ccgttgacga tgtcgacagg
     5701 atagtactga tgataattca ttatttcgcc tggcttatca gtccgcgtgt aggctgcatc
     5761 cggtaatcaa tgcctgatgc gacgctgtcg cgtcttatca ggcctacagc cgttgccaaa
     5821 tgtaggccgg ataaggcgtt tacgccgcat ccggcatttg cttagcgcag agtgaagatt
     5881 aacctatctg cccattcccc aaatgtttaa cgcggcgctt aacttcttcc tgcttaccag
     5941 cgttaaacgg acgtgcatcc gggctaccta aatatccgca cacgcggcga gttaccgaca
     6001 cacgggaggc gtcatggtta ccacatttcg ggcaagtgaa gcctttgctg gtgcactcga
     6061 actcaccggt aaagccacac tcgtagcact catcaatcgg tgtattggtg ccgtaatacg
     6121 gtacatgctg atagctgtaa tcccagacat cttccagcgc tttcaggttg tgctgaatgt
     6181 ttggatactc gccgtagcaa atgaaaccac cgttcgccag cggcgggtaa ggcgcttcaa
     6241 agtcgatctt gtcgtacggg ttcaccttct tctccacatc aaggtggaaa ctgttggtgt
     6301 agtaaccttt atcggtcacg cccggcacca cgccaaactc agcagtatcg agacggcaga
     6361 agcggtcgca caggttttca ctcggcgtgc tgtaaagact gaaaccataa cccgtttctt
     6421 ctttccactg atccactgcc tgacgcagac gttcaacaat ggcgatacct ttcgcgcgaa
     6481 gcttctcgtt gtcgtaaaca tgctcgccgc tgaacagcgc gttaatggtt tcgtggatgc
     6541 cgatgtaacc cagcgaaata gacgcacgac cgtttttgaa gatttcagaa acatcatcgt
     6601 cagcattcag acgtacgcca caagcacctt ccatatagag gatcggggcg acgcgcgctt
     6661 tcacgccttc gagacgagcg atacgggtca tcagtgcctt acgtgccagc accagacgtt
     6721 catccagcag cttccagaag gtggcttcat cgccttttgc ttccagagca atacgcggca
     6781 ggttcaggct gatcacgccg aggttgttac gaccatcgtg gatctgctcg ccgttttcat
     6841 tttcccacac gccgaggaag ctgcggcagc ccatcggggt tttaaacgaa ccggtgacgt
     6901 tcactacctg atcgtagttc aggatatccg gatacatgcg cttgcttgcg cactccagcg
     6961 ccagctgttt gatgtcgtag ttcggatcgc cttttttatg gttcaggcca tcgcgaatcg
     7021 caaacaccag tttcgggaac accgcagttt tacggttttt accgaggcct gcgatacggt
     7081 tacgcaggat cgattcctga atcaggcgcg attcccagct ggtgcccaga ccaaaaccaa
     7141 aggttacaaa cggcgtctga ccgttggcgg tgtgcagggt gtttacttcg tactccagcg
     7201 actggaaggc atcgtagcac tctttgatgg tacgagagtt agcgtagcct tcggcgtccg
     7261 ggatgttcca ctcttctgcg gtcttgcgat gtttgttgta gctggcggta acaaacggcg
     7321 ccagcacttc atcgatacgg ttaatggtgg tgccgccata aatatggctg gcgacctgag
     7381 caataatctg cgcagttacc gcggttgccg tagagatcga cttcggcggt tcaatctcgg
     7441 cgttccccat tttaaaaccc tgggtcagca tgcctttcag gtcgatcaac atgcagttga
     7501 acatcgggaa gaacggcgag taatcgagat cgtgatagtg aatatcgcca cgctcatgtg
     7561 cctgcaccac gtcacgcggc agcaggtgct ggcgtgcata gtgtttagcc acgatcccgg
     7621 ccagcaggtc gcgctgggtt ggaatcacct tgctgtcttt gttggcgttt tcgttgagta
     7681 acgaggcgtt ggtctgctcg accagaccac ggatctcctg gttcaggcga ccgcgttttt
     7741 cacgttcaat gtcgcgatcg tgacggtact cgatgtaagc acgagccagt tgtttgtatg
     7801 gacccgacat cagctggttt tcaactgcgg tctggatctc gttgatatcc acctggttgc
     7861 gcccctgcat ctgctcgttg acaaccgcgg caacagtggc gcaataatcg gcatcatcga
     7921 cttccgctgc tttagctgca cgcagaatcg cttctttgat gcgctctgat ttaaacggca
     7981 ctttgcagcc gtctcgtttc atcacatgcg gtgtcatgat cgctccatat ttttaagaac
     8041 aggttatcca cagaaattgg gaaaggcgtt ttccggtttt ttcgtttcgt tttccggtgc
     8101 tttccgcaat cgccatccgc ttatccacaa cgccgcacct ttttcgtggg gagctgtagt
     8161 agcaattata gtcgattaat acaacatatt gggttgggac gcattttaaa gtctatatat
     8221 agtgctttgc atcaaggatg ttttaacttt ttttgatgta gctcaaagta aaaagcagag
     8281 cgtacggatg acgggcgcta cagcgttatg taaatttttt aatgaatttg ctggttgaaa
     8341 aatcaacaaa aacaacatgc tgacagacaa aaccccggaa tgaccggcat tatcggggct
     8401 tagggaagat ttacttctgt aaccaccaga cagcctcaaa aggccgtaaa ttcatggcac
     8461 agggttgtgg tgaggcttct tcgtagttat gcatcacaag ctgccagttg ccgcgcattt
     8521 gccctggctg ccagggttgg atctcacggc taaggttggc aatgaccagc aaggtttgcc
     8581 ccttccattc acggcgatag caccacaata cagggctgtt tggcagcaga tcctggtaat
     8641 tgccccatgt caggatggct tcttgcttac gcagtgcgat taacttttgg taggtgtaaa
     8701 acacagagga gtcatcggcc agcgcggctt ctacgttgat ttgttgatag ttatcgccta
     8761 ggccaatcca cggttcgcca gccgtaaacc cggcattatc gccgttgctc cattgcatgg
     8821 gcgtgcggct gttgtcacgg gatttactgg cgaggattgc caataactcg tcggcatcac
     8881 gaccatcgtt gcgcagctcg gcaaacatat tgaggctctc cacgtcgcga tagtcagtaa
     8941 tgcgcgtgaa atgcgggttg gtcatgccaa tctcttcgcc ctggtagata tacggcgttc
     9001 cctgcatgcc atgcagcacc atcgccagca tttttgccgc aggcacgcgg tattcacctt
     9061 catcaccaaa gcgagaaaca atgcgcggct gatcgtggtt acaccagaac aaggcattcc
     9121 atgccacgtt gtgcattcct tgttgccagt ggcggaacaa tgttttcaac gccacaaagt
     9181 caggtttagc cagcgtccat ttttcaccac cgggataatc gaccttcagg tgatgaaaat
     9241 taaaggtcat cgacaattca ctgcctgtca gtgccgcgta tcgctggcaa tgctcaaggc
     9301 tggtggagga catttcacct acggtcatta acccgcgtgg cgtaaacaca tcgcggttca
     9361 tctcgtgcaa aaactcgtgt gctcgtggcc cgtcggtgta gaagcgacgc ccgtcgccat
     9421 ccaggtcttc agggaaacgc gggtctttgg agatcagatt caccacatcc aggcgcaacc
     9481 cgtcgacccc acgatcggcc cagaattcac agactttttt cagctctgcg cgtaccgctg
     9541 gattctccca gttgagatcc gcctgttctg gtgcaaagag atgcaaatag tactgttcgc
     9601 tttccgcatg ccagcgccac gcactaccgc caaattttga acgccagttg ttcggtggcg
     9661 tttctggttc gccatcgcgc cagatataaa actggcggta agggctttct ttgttcagcg
     9721 cctcgcgaaa ccaggcatgt tgggtagagg tatggttaaa caccatatcg agaatgatac
     9781 gaatcccgcg cgattttgcc tgcgtcacca gttcgtcaaa atcgtccagc gtgccgtagg
     9841 tgggatcaat cgccgtatag ttcgctacat cgtaaccgtt atcgacctgg ggagagacat
     9901 aaaagggggt tagccagatg gcatcaacgc ccagtttatg cagatagtcc aggcgttgga
     9961 taacgccacg taaatcgccg gtgccgctac ccgtggtgtc ctgaaaactc tttggataaa
    10021 tctggtagat aacgccgttt tgccaccagt ggggaagatt agtcattacg ttattcctgc
    10081 aaatgcgagg gggcgcaatt gcgccccgaa gaaaattaaa caatgtccag cgtgcccagg
    10141 cggtatttcc gctgatagat aaacgaggtg agtacaatcg ggatgatgat ggcgatagcc
    10201 attgccagcg caaatacctg ccagtagctc ggttgaatag agagaattcc cggcaggccc
    10261 cctacgccga taccattcgc cataacgccg ttcaggccgc atagcaatcc tgccagacca
    10321 gaaccaatca tcgcgcacag catcgggaag cgatatttca ggttgatgcc gtacattgca
    10381 ggctcagtga ccccaagcca ggcggagata gcggcaggca cggagatctc gcgttcattg
    10441 tgcttgcggc tggaaatgat aatgcctatc acggcggagc cctgagcgat attcgacagc
    10501 gcaatcagcg gccacactgg cgtgccaccc atgctttgaa tcatctgcaa atcaatagca
    10561 agggtggtct ggtgtacgcc ggtgatcacc agcggcgcgt acaggaagcc aaacaatgcc
    10621 gcaccaatcg gagcaaagct gccggtcatc aggtgacgta ccgcaaaggc aacgccatcg
    10681 ccaatcatgc gaccaaacgg accaatcagc gcatgggcga ggaacaccgc gaggatcagc
    10741 gaacagacgg gcaccaccac cagatagagg taatccggta cgatgcgttt aaggcgagtt
    10801 tcaataacgc ccagcgccag cccggctaac agtgccggga tcacctgcgc ctgatagccc
    10861 actttggcga tgctgaacat gccaaagtcc cacacttccg gcagctgctg cccgagcaga
    10921 taagcgttca tcagctgggg ggaaaccagt gtcacgccaa gcacgatacc aaggatcggc
    10981 gtgccgccca tttttttcac cgctgaccag caaatcccga ccggcaggta gaagaaaatc
    11041 gcttcaccga tcaaccacag aaaatcgtag atcgtttgca gggaagggta catttgcgcc
    11101 agcgtctgac cgttgctcat gggcaaatcg ccgatcacat tgcgaaaacc gaggatcaaa
    11161 ccgccgctaa tcaacgcggg cagcaacggg aagaagatct ccgcgaaatg agagatcaac
    11221 tgctcatgcc atttcatatt ttgccgggcg gcttttttta cctgctcttt atcaacctgc
    11281 gcctgtccgg ttgacgctat tagtgcttga tagtaatcac ccacgttggt gccaatcacc
    11341 acctgaaatt gcccggcatt ggtgaaacag cctttcacca tggggagttg ctcaatttct
    11401 ttcggtctgg cattggccgg ttggttgagg acaaagcgca ggcgagtaat acagtggctc
    11461 accgtcgcaa tattgccgcg cccgccgacc agttcaatca accgatcgat atccgtttgg
    11521 tttattttgc tcatcataaa gccccatggc agatgacatt tttggttggc tgcagaatat
    11581 attgcgcgga aaaatttaaa aacgggaacg ttcccgaaac gcagcgaaga tcacaattta
    11641 tcgttcagga aacgatcagg acagggtggc ggggatgatg atttgttgcg gttcgctgcg
    11701 cccggttacc tgtgcgatca attggcaggc cgcctggcgt ccagcttcgg cgtagccagg
    11761 atctacggtg acgatctccg gatgaaggaa tttcattaac ggcgtattac ctacgctcgc
    11821 cagttgcaag gtgtcgatgc gttgctcttg caggtattta cttgcgccaa gagccagcgt
    11881 gtcggttgcg cacagtaacg cggtagtttc aggcgtaatc acttttgcta cgttttcata
    11941 gccttgcttc atagcaagcc ctggcagagc ggcaacggga tgcagtttat gcgctttgca
    12001 gaacgccagg taggcttcgt gacgtcgctt cccggtcgtc acgtcactat gcggcacgcc
    12061 gagataactg atattgcgat gcccctggtc atagagccgt tgcatcagaa tcttgattgc
    12121 cccttcgtcg tcataacaga ccgaagcaaa gccttttgcg tcacgcgcca gcagaaccag
    12181 cgatgactgc cagtgggcta acatttcttc ggttatgccg gtaaaaccga acagcactac
    12241 gccgtcgata ttacgccgtt tcagcacccc caaatgttcg gcaactaatt gtggggaaaa
    12301 ctgattttcc atcatgattg gatcgtaacc ttgttcatag aacgctggca gcatggtttg
    12361 aacggcgaga ttttctgaca acgaatccag acgggtaaca atgatggcga ccactttatc
    12421 gctttgccca cgcatagcgc gcgcagagcg ggaaggggaa aatccatgct gattcatcac
    12481 tgcttcaaca cgctcgcgcg tgcgctgact cacgccgctt tcgttattca gcacccggga
    12541 aaccgtagat ttccccacgc cgcttaagcg cgcgatgtct ttgatggtca gccgattttg
    12601 catcctgttg tcccataacg tgttgtttaa ttatttgagc ctaactttac ccgtgcattc
    12661 agcaatgggt aaagtctggt ttatcgttgg tttagttgcc agcaggtatt atatcgccat
    12721 aaatgctacg aatattattg gattctcctt attatttgcg gcgctttttt cacttaccgg
    12781 aggttatatg gaacctgatc ccacgcctct ccctcgacgg agattaaaac ttttccggta
    12841 agcccgtctt ttcacggcgt taccggatgc gtaaggccgt gacgttttaa tgtccctgct
    12901 cagctttatt accttcaggt aaggcttcgc cacgcctgaa gacatttctg tactgtttca
    12961 gacagtgcgg agggactcct tatgtttaaa gaaattttta cccggttcat tcgccattta
    13021 ccttcccgtc tggttcatcg cgatccattg cctggcgcgc agcagacagt gaatacggcg
    13081 gtcccgccgt ccttaagtgc gcattgcctg aaaatggcgg tgatgcccga agaagaattg
    13141 tggaaaacgt tcgacaccca tccggaagga ttaaatcagg cggaagtgga atctgcccgc
    13201 gaacaacatg gtgataataa attacccgcc caacaaccgt cgccgtggtg ggtacattta
    13261 tgggtctgct atcgcaaccc ctttaatatt ttgctcacca ttcttggtgc tatttcttac
    13321 gccacggaag atttatttgc cgcaggtgtt atcgcgctaa tggtcgctat ttctacgttg
    13381 ctgaacttta ttcaggaagc tcgttccacc aaagcggcag atgccctgaa agcgatggtc
    13441 agtaataccg cgacggtact gcgagtaatt aacgacaaag gcgaaaatgg ctggctggag
    13501 atcccgatag accagctggt gcccggcgat attataaaac tggcggcggg ggatatgatc
    13561 ccggcagatt tacgtatctt gcaggcgcgg gatctgttcg tcgctcaggc gtcgttaacc
    13621 ggtgagtctc tgcccgtgga aaaagccgct actactcgcc agccggagca cagcaatccg
    13681 ctggagtgcg acaccctgtg ttttatgggc accaccgtgg tgagcggcac ggcacaagca
    13741 atggtgattg ctacaggagc caacacctgg tttggtcaac tggcggggcg tgttagtgag
    13801 caggaaagcg agccgaatgc ctttcagcaa gggatcagcc gcgttagtat gctgctgatt
    13861 cgctttatgc tggtgatggc tccggtggtg ttgcttatca acggttacac caaaggcgac
    13921 tggtgggaag cggcactgtt tgcgctttcg gtcgcggtag gcttaacgcc ggaaatgttg
    13981 ccgatgattg ttacctcgac gctggcgcgc ggcgcagtaa agctgtcgaa acagaaagtg
    14041 atcgtcaaac atctggatgc cattcagaac tttggcgcaa tggatattct gtgcactgat
    14101 aaaaccggca ccctgacgca ggacaaaatt gtgctggaga atcataccga tatctccggt
    14161 aaaaccagcg aacgcgtgct gcatagcgcg tggttgaaca gtcattacca gaccggactt
    14221 aaaaatctgc ttgataccgc ggtgctcgaa ggtacggatg aagagtcagc gcgttcgctg
    14281 gccagccgtt ggcaaaaaat tgatgagatt ccgtttgatt tcgagcgtcg tcggatgtcg
    14341 gtggtagtgg cagaaaatac cgaacaccat cagctggttt gcaaaggtgc attgcaggaa
    14401 atcctcaatg tgtgttcgca ggtgcgtcac aatggcgaga ttgtaccgct cgatgacacc
    14461 atgctgcgta agattaagcg ggttactgat acgctgaatc gtcaggggct gcgcgtggtt
    14521 gcggtggcga cgaaatacct gccagcgcgt gaaggagatt accagcgggc ggatgaatcc
    14581 gacctgatcc tcgaaggata tattgctttc cttgatccgc caaaagagac aaccgctccg
    14641 gcactgaagg cattaaaagc gagtggaatt accgtaaaaa tccttactgg cgacagtgag
    14701 ttagttgcag cgaaagtgtg ccatgaagtg gggctggatg cgggagaggt ggtcattggt
    14761 agtgatattg aaacgctatc tgacgatgaa ctggcaaatc tcgcacagcg caccacgctg
    14821 tttgcccgcc tgacgccgat gcataaagaa cgcattgtga ctttactgaa gcgcgaaggg
    14881 catgtggttg gctttatggg cgatggtatt aatgatgcgc ccgccttacg cgctgcggat
    14941 atcggcattt ctgtggacgg cgcggtagat attgcccgtg aagcggctga tatcatcctg
    15001 ctggaaaaaa gcctgatggt gctggaagag ggggttattg agggacgtcg cactttcgcc
    15061 aacatgctga aatacatcaa attgacggcg agttctaact tcggtaatgt gttcagcgtg
    15121 ctggtggcga gtgctttctt gcccttcctg ccgatgttgc cgttacactt gcttattcag
    15181 aacctgctgt acgatgtgtc acaggtggcg atcccgtttg ataacgtcga cgacgagcaa
    15241 attcaaaagc cgcagcgttg gaatccggcg gatctggggc gctttatggt cttcttcgga
    15301 ccgatcagct cgattttcga tattctgacg ttttgcttga tgtggtgggt attccatgcc
    15361 aacacgccgg aaacgcaaac gctgttccag tcgggctggt ttgtggtggg attactgtcg
    15421 caaacgctga ttgtgcatat gatccgtacc cgccgtgtgc cgtttattca gagctgtgca
    15481 tcgtggccgt taatgatcat gaccgtgatc gtgatgattg tcgggatcgc attgccgttt
    15541 tcaccgctgg ccagttatct gcagctgcag gcgctgccgt taagctattt cccgtggctg
    15601 gttgcgattc tggcagggta tatgacgtta acccagttgg tgaaagggtt ctatagccgt
    15661 cgttacggct ggcagtaaag aataaactgg gcatggtggc ccggattcag attgcagaca
    15721 aagtgagctt tgttcatgcc ggatgcggcg taaacgccca atccggccta caaattcaat
    15781 aaattgcagg aatcacgtag gcctgataag cgtagcgcat cgggcgattt tgcttttgtc
    15841 atcagactta atccgggcat gatagcccgg atttccatca agattagcga cgaacagcga
    15901 ttgcttcgat ctcaatcttc acgtctttcg gcagacgggc aacttcaacg caagaacgtg
    15961 ccgggaaagt ggcgttgtgt tcggtgaaga aggcttcgta agtggcgttt acggttgcga
    16021 agtcgttcag atctttcacg aacacagtgg ttttaacgat gtcgcccact ttcaggccag
    16081 cggcttcgac gattgctttt acattatcca gcgactgacg tgcctgtgca gcgacgtctg
    16141 ccggtacttc accagttttc ggatttaccg ggatctgacc ggaggtgatg atcatattgc
    16201 ccagatcaac gccctgtacg taaggaccga tagctgccgg tgcattttcc gtcgcgatag
    16261 ttttgctcat gatttctcct ttattacagc ggtaaaaagt ctggctcatt atagggagcc
    16321 agacttttat taccaaccgc aattaattgg ccagcaccac attatgggaa aactcttttt
    16381 cacagtattt gcatttaagc gcgatgtcat tggcgcgttt tcgcacggca aagctggatg
    16441 aaaccggttc ggcatggctg atacagttgc tgttcgggca gaccagcaca ttgtcgatgc
    16501 gctctggcag acttgggcgc gatttaccca ccacttcata gttgtcgata cggttaaccg
    16561 tggcttgcgg tgcatacaat gccagttggt ctacttgctc ttcactcaaa aaggtatttt
    16621 cgattttgat cagatctttg cggcccatct cgccagaagg caaattcaga ccaatggtga
    16681 tgcgctgatc tgtttcggtc aacttgaaca gactcaacag cttaaaaccg atctgggcgg
    16741 ggatatggtc aattaccgtg ccgcgtttaa tagcttcaac ctgcaattta ttatcgtgtg
    16801 tcatctctat ttccccttac agtaccagat cgcgattcag aaccagtgcc agtaacgcct
    16861 ggcgagcgaa aatcccgttg cctgcttgct ggaagtacca ggcgtgaggc gttttatcca
    16921 catccgtcgc aatctcatca acacgcggca gcggatgcag cactttcata ttggctttgg
    16981 cgttgtggag atcgctggcg cgaagaacaa actgcgcttt cacgttggcg tactcggacg
    17041 ggtccagacg ctctttttgc acgcgggtca tgtacaggat gtctacttcc gccatcactt
    17101 cttcaataga gctgtgcaga ctccaggcga tccctttttc atcgagcata tccagaatgt
    17161 attgcggcat tgccagcgca tccggcgcga taaagtaaaa acggttgccg tcgaacttcg
    17221 ctagcgcctg ggtcagggag tgaacggtgc ggccatattt caggtcacca accattgcga
    17281 cgtggagatt gtccagacgc ccctgggttt cctgaatggt gaataagtcc agcaaggttt
    17341 gcgtcggatg ttggttggag ccatcaccgg cattcagtac cggtacattg ccagaaaatt
    17401 cggtggctag gcgtgccgca ccttcctgcg gatgacgcat cactatcgca tcgacgtaag
    17461 tgctgataac cgaaatggta tcggccagcg tttcgccttt tttacccagt gatgtattgg
    17521 cgctgtcgga gaagcccacc acgctggcac ccaggcggtg catggaagtt tcgaaagaga
    17581 ggcgggtacg ggtagaggct tcgaagaaac agctggcaat gactttgtgc ttcaacagct
    17641 ctggttgcgg gtttgctttc agtttcgccg ctgtcgccag caccagatta aggtcatcgc
    17701 gactaaggtc gtttatggaa atgatatgtt tctgatatag cggattagcc atcttttatc
    17761 tcctgacacc taggcaaaaa aaagcccctc gattgagggg ctgggaatgg gtgatcaacg
    17821 ggaagaaaaa cggcaggcca gcgtcttttt tcagacgcgg taagacaaaa tgtcgaacac
    17881 actgaaccat acatcctccc ggcaaattgt ccggcattat actcatagcc agaagcggtg
    17941 caagcatttg atgcaatatt ttgtcagcgc aaacggttta tttgaattaa aagtcaaaat
    18001 atatgcattt ttattttcta tgattctgga ggggaagtgt cgttcggtat caataattgc
    18061 agacatttcc tgcttttttc tttttcattt tcacgcaatc agaaatgcat tttattccat
    18121 tcatccgact tatagggcgg agggtgaaaa cgaacggcta acactattgc ttatagctca
    18181 gggatacgcg ctatcacttt aatttcaaaa tcaaagcctg ccagccaggt aacacccacc
    18241 gccgtccagt ttggataagg tggggcgcta aatatttcat ttttcaccgt catgatgtct
    18301 tcagattgtt tttccggatc ggtatgaaag ctcgtaacat caatgatatc gtcaaaagtg
    18361 catcccgcag ctgccagggt cgcatgcaaa ttatcaaagg ccagtctgac ttgttgctga
    18421 aaatcgggtt ctggtgttcc gtcctctcga cttcctactt gcccggaaac aaacagcaaa
    18481 tcgccggaac gaatagccgc agaataacga tgctcagcat acagtgaatg tcggccagca
    18541 gggaaaacag cggttctttc taccatttgg ttatcctcaa gatttacgac atgaacagaa
    18601 gatttatatt tatcgggagc cgcttttagg gggacgacgt tagtaaacaa aacccagaca
    18661 tcatggataa tggctgggct taattgagcg tagtcggtta tgcgccaaac gcgccatcaa
    18721 tggtatgcat cgcgccggta acaaaactgg cttctggccc tgctaaccat gcgaccatac
    18781 cagcgacctc ttccggttgc ccatgtcttt tgatggccat aaaactatgc aacatatcgc
    18841 gcattggccc gttggcggga ttaacgtcgg tatcaattgg tcctggctgg acgacgttga
    18901 tggtgatccc tcgcggtcca aaatcacggg ccagcccgcg cgccatgcct tgcagggcag
    18961 atttactggc ggcataagca gccatgcctg caacaggcat acgatcgcca ttcacggagc
    19021 cgatgattaa gatgcgcccg ccttcgggca tctgccgggc ggcttcaaca gaggcatgat
    19081 aaggagcatg aatattgatt ttgaaaaggc gatcaatatc gtcggcattt aattccaggg
    19141 catcgccaaa gacgccaata cctgcattta ccaccaggat atccaatgcg ccgctcttac
    19201 gaacgacatc aataacagcg tctctgtcag cactatctgt gaatactgct gtcgctccag
    19261 tctcttgtgc cagacgttca gcggcatctt tcgaccccgc ataggtgaat cgtacattgg
    19321 ccccatcggt gacgaaacga cgtacgatag cggcaccgat tccacgactg ccaccgagga
    19381 tgagaactgt cttaccggta aaagcgccca taaggactcc ttgatttatt atgtaacatg
    19441 cattacaaaa ctgttttaac tttctgtcaa caggttttgt aatggtcact aaaaaacaat
    19501 ctcgcgttcc aggtcgtccc agacgtttcg ctcctgagca ggcagtctct gcggcaaaag
    19561 tgctttttca ccaaaaaggt ttcgatgcag taagtgtctc tgaagttact gattatcttg
    19621 gtattaaccc cccgagtctc tacgcggctt ttggcagtaa ggctgggtta tttagccgtg
    19681 tactcaatga atatgtcggt acggaagcta ttccgcttgc cgatattctt cgtgatgatc
    19741 gtccggtagg cgagtgcctg gttgaggtat taaaagaagc ggcgcgcaga tatagccaaa
    19801 acggcggctg cgctggctgt atggttcttg aaggtattca tagtcatgat ccacaagcgc
    19861 gtgatattgc cattcaatat tatcacgccg cagaaacgac catttatgac tatatcgcca
    19921 ggcggcatcc acaaagcgca caatgtgtga ctgattttat gagtaccgtg atgtcagggc
    19981 tttctgcgaa ggcacgggag gggcactcca tcgaacaact ctgtgcaaca gctgccctgg
    20041 caggggaagc gataaaaact cttctcaagg agtgatgctg gccttgatcc gaaaggcggg
    20101 aacgcgcctg ccgataagtt gtgataagac aataattcac gcgttaaggc tggcggaatt
    20161 gatcatcttt tcgtataacg atagaaatga aacattgttt taattaagga gtggaaaaga
    20221 tgatcatcgg aaatattcat aatcttcagc cgtggctacc ccaggagtta cgccaggcga
    20281 ttgagcatat caaagcacac gttacggcag aaacgccaaa gggcaagcat gatatcgaag
    20341 gcaatcgtct gttttatctt atctcggaag atatgaccga gccgtacgaa gctcgccgtg
    20401 cggagtacca tgcccgctat cttgacattc agattgtgtt aaaaggtcag gaaggcatga
    20461 ccttcagcac gcaacctgca ggcgcgccgg ataccgactg gttagctgat aaagacatcg
    20521 catttttgcc ggaaggcgtt gatgagaaaa cagttatcct aaatgaaggt gattttgttg
    20581 tgttttatcc gggggaagtg cataaaccgc tgtgcgcagt gggcgcgcca gcccaggttc
    20641 gcaaagcagt agtgaagatg ctgatggcgt gatgactttt cgccgtaaat aactccaggt
    20701 ttacggcgag tttgtgaaaa gagcgttttt tgatattttt ttgtgagtaa aatttgtaat
    20761 gcttagacgt tcttattaac tcaaggagtt cgtcatgggc aaaatatcag atttgaatta
    20821 ttctcaacac attacattag ccgacaattt taaacaaaaa aatgaagcct tagatacctg
    20881 gtatgtggga atgaatgatt ttgcccgaat tgccgggggg cagaatagca gaagcaatat
    20941 tctttctcct agagcatttt tggagttttt ggcaaagata tttaccctgg gttatgtgga
    21001 ttttagcaaa cgctccaacg aagcgggtag aaatatgatg gctcatattg agttctcatc
    21061 ttattctaaa gatactgatg gcaatgaaaa aatgaagttt tacatgaata atcctgaagg
    21121 ggagcgagcg gatttatcta aggtgaagat agaaattaca cttgcctcag cttctactaa
    21181 agggattcgt gaaggacata cagtcattat atttaaacaa tctgatggtt cgactaatcg
    21241 ttatgaaggg aagtcttttg aaagaaaaga tgatagttca ttacacctga ttactaacaa
    21301 ggttctggcg tgttaccaac gcgaagctaa caagaaaata gcgcgtctat taaataatca
    21361 tcagaagtta aataatatac aggagttaaa tgattcgcag gagttaaata attcacagaa
    21421 gttaaataat tcgcaggagt taaataactc gcaggtgagt tgtaaaggtt cagttgattc
    21481 tacgattacg gatttattag aaaaagcatt gaa
//
LOCUS       DADLTY010000063        21409 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.063, whole genome shotgun sequence.
ACCESSION   DADLTY010000063 DADLTY010000000
VERSION     DADLTY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.063"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            24..524
                     /gene="tnpA"
                     /locus_tag="JN334_001962"
     CDS             24..524
                     /gene="tnpA"
                     /locus_tag="JN334_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAY7438112.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(563..910)
                     /gene="grxD"
                     /locus_tag="JN334_001963"
     CDS             complement(563..910)
                     /gene="grxD"
                     /locus_tag="JN334_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HAY7438113.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1245..2072
                     /locus_tag="JN334_001964"
     CDS             1245..2072
                     /locus_tag="JN334_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7438114.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2200..2781
                     /gene="sodB"
                     /locus_tag="JN334_001965"
     CDS             2200..2781
                     /gene="sodB"
                     /locus_tag="JN334_001965"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAY7438115.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(2927..4102)
                     /locus_tag="JN334_001966"
     CDS             complement(2927..4102)
                     /locus_tag="JN334_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7438116.1"
                     /translation="MKINYPLLALALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAA
                     GMLISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSR
                     ILTSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGW
                     RMSFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGA
                     MFTLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLL
                     LLMVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIG
                     AFNLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4268..4357)
                     /gene="ynhF"
                     /locus_tag="JN334_001967"
     CDS             complement(4268..4357)
                     /gene="ynhF"
                     /locus_tag="JN334_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HAY7438117.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            4656..5681
                     /gene="purR"
                     /locus_tag="JN334_001968"
     CDS             4656..5681
                     /gene="purR"
                     /locus_tag="JN334_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HAY7438118.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(5678..6610)
                     /locus_tag="JN334_001969"
     CDS             complement(5678..6610)
                     /locus_tag="JN334_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7438119.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            6723..7934
                     /locus_tag="JN334_001970"
     CDS             6723..7934
                     /locus_tag="JN334_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HAY7438120.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8225..9373
                     /gene="cfa"
                     /locus_tag="JN334_001971"
     CDS             8225..9373
                     /gene="cfa"
                     /locus_tag="JN334_001971"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAY7438121.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9413..10054)
                     /gene="ribE"
                     /locus_tag="JN334_001972"
     CDS             complement(9413..10054)
                     /gene="ribE"
                     /locus_tag="JN334_001972"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAY7438122.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10269..11642
                     /gene="mdtK"
                     /locus_tag="JN334_001973"
     CDS             10269..11642
                     /gene="mdtK"
                     /locus_tag="JN334_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HAY7438123.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(11683..12939)
                     /locus_tag="JN334_001974"
     CDS             complement(11683..12939)
                     /locus_tag="JN334_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438124.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13247..13323
                     /locus_tag="JN334_001975"
     tRNA            13247..13323
                     /locus_tag="JN334_001975"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13281..13283,aa:Val,seq:gac)
     gene            13328..13404
                     /locus_tag="JN334_001976"
     tRNA            13328..13404
                     /locus_tag="JN334_001976"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13362..13364,aa:Val,seq:gac)
     gene            13512..13817
                     /locus_tag="JN334_001977"
     CDS             13512..13817
                     /locus_tag="JN334_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAY7438125.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            13943..15365
                     /locus_tag="JN334_001978"
                     /pseudo
     CDS             13943..15365
                     /locus_tag="JN334_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(15377..16189)
                     /locus_tag="JN334_001979"
     CDS             complement(15377..16189)
                     /locus_tag="JN334_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438126.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(16193..16978)
                     /locus_tag="JN334_001980"
     CDS             complement(16193..16978)
                     /locus_tag="JN334_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HAY7438127.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(16975..17643)
                     /locus_tag="JN334_001981"
     CDS             complement(16975..17643)
                     /locus_tag="JN334_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7438128.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(17707..18345)
                     /locus_tag="JN334_001982"
     CDS             complement(17707..18345)
                     /locus_tag="JN334_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HAY7438129.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(18358..20459)
                     /locus_tag="JN334_001983"
                     /pseudo
     CDS             complement(18358..20459)
                     /locus_tag="JN334_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(20480..21106)
                     /locus_tag="JN334_001984"
     CDS             complement(20480..21106)
                     /locus_tag="JN334_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HAY7438130.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         5343 a         5553 c         4998 g         5515 t
ORIGIN      
        1 cctgtaatcg tttcacaggt caaatgatac tttggtctgt gaaaaaagaa accgatatcc
       61 ggcgtggaag gcattgtgtt ttcctgatgc atgttcacct ggtctttgtc accaaatacc
      121 gacgccagat ttttgaccat gacacgacag aaaaactacg cacttacttt tcaaatgtat
      181 gtgctgattt tgcagctgaa ctggttgaaa tggatggcga accagatcac gtccatttgt
      241 taattaacta tcctcccaaa ctggcgatat ccagtctggt aaacagcctc aaaggcatat
      301 cgaataggtt actgcgacga gatcgaccag atattgcagt caggtattac tacaaaggcg
      361 ttttgtggag tcctggctat tttgccagta gctgcgaagg tgcgccaata tccgtcatcc
      421 gccagtacat tgaacaacag caaacacctg gtcaggtgga aaaccgcgcc ttatatcccc
      481 gccctgaagg acgaggtttt acggcgcacc ggataaagga ctgacggatg cccgttcgca
      541 tccgtcagta ttgcaggacg gattattccg cgtccggctc ttcagacttg tatttagcgg
      601 cagtttcttt gatcagctgc tgcagttcgc cacgctgata catttcgatc acgatatcac
      661 aaccgccgac cagctcgccg tcaacccaca gttgcgggaa ggtcggccag ttagcatatt
      721 tcggcagttc cgcacgaatg tccggattct gcagaatatc aacataggca aaacgttcgc
      781 cacatgcggc aagcgcctgg actgcctggg cagagaaacc gcagctcggc agtttcggtg
      841 aacctttcat gtacagcagg atcgggtttt cagcaatctg gcgttggatt ttttcgatag
      901 tggtgctcat tgtcttgctt cctcaaactt ctttacggca gtaatctgac attgtagcgg
      961 gtcagtgcgg catcggaaaa taacattttc atcacgcttt tgctatttta tccctttgct
     1021 ctatttgttg catttcaaat attcgtttct tctctgatgc gcggcaaact ggcgtattta
     1081 taaccatttt tagctttcac tgctgcattt ttttgcgctc gccaacgaaa cgtatttttt
     1141 aacaataaaa gctattaact ttctcttctt ctatgcatta gaatcatcaa gttttgtaaa
     1201 tcagacgcag gcatgataga cctgccttaa cagagggacg ctcagtggcg cggataaacc
     1261 gtatttcgat cacgctctgt gctttacttt ttaccaccct gcctttaacg cctatggccc
     1321 atgcttcaaa gcaagccagg gagagttctg ctaccactca tatcaccaaa aaagcagata
     1381 aaaagaaaag cacggcaacc accaaaaaga cccagaaagc cagccagaaa acagcgaaaa
     1441 aagccgccag taaaagtatg accaaaagca aaaccgcttc ttctgttaaa aaatcttcca
     1501 ttaccgcttc taaaaacgcc aaaactcgca gcaaacacgc cgtcaataaa acggcctcag
     1561 ccagctttac cgaaaagtgt accaaacgta agggctataa atcgcattgt gtgaaagtca
     1621 aaaatgccgc gtcaggaacc cttgccgacg cgcacaaagc gaaggtgcaa aaagccacga
     1681 aagtggcaat gaataaactg atgcagcaaa ttggtaagcc atatcgttgg ggcggcagct
     1741 caccgcgtac cggttttgat tgcagcggcc tggtttatta cgcttataaa gatttggtga
     1801 aaattcgtat tccgcgtacg gcgaatgaaa tgtatcacct gcgtgatgca ggacctatcg
     1861 aacgtagtga gctgaaaaac ggcgacctgg tctttttccg tactcaggga cgcggtacag
     1921 ccgatcatgt cggcgtgtat gtcggcaacg gcaaatttat tcagtcaccg cgcacaggtc
     1981 aggaaattca aatcacttct ctcagtgaag actactggca gcgccactat gttggcgctc
     2041 gtcgggtaat gaccccaaaa acacttcgct aaaactttac cctgttgtta cggcaacagg
     2101 gtaagttcat cttttgtctc accttttaat ttgctaccct atccatacgc acaataaggc
     2161 tattgtacgt atgcaaatta ataataaagg agagtagcaa tgtcattcga attacctgca
     2221 ctaccatatg ctaaagatgc tctggcaccg cacatttctg cggaaactat cgagtatcac
     2281 tacggcaagc atcatcagac ttatgtcact aacctgaaca acctgattaa aggtaccgcg
     2341 tttgaaggta aatcactgga agagattatt cgcagctctg aaggtggcgt attcaacaac
     2401 gcagctcagg tctggaacca tactttctac tggaactgcc tggcaccgaa cgccggtggc
     2461 gaaccgactg gaaaagtcgc tgaagctatc gccgcatctt ttggcagctt tgccgatttc
     2521 aaagcgcagt ttactgatgc agcgatcaaa aactttggtt ctggctggac ctggctggtg
     2581 aaaaacagcg atggcaaact ggctatcgtt tcaacctcta acgcgggtac tccgctgacc
     2641 accgatgcga ctccgctgct gaccgttgat gtctgggaac acgcttatta catcgactat
     2701 cgcaatgcac gtcctggcta tctggagcac ttctgggcgc tggtgaactg ggaattcgta
     2761 gcgaaaaatc tcgctgcata ataactgatg gcaaatgcag cattgcctga tgcgcttcgc
     2821 ttaccatgcc aacgcggatg aggcgttaac agcgcattcg gcaagataaa tcgcacgttg
     2881 tcagcaactg taacgcagaa ggttatcctt ctgcgttttt gtttaattag ctgttagcaa
     2941 cgcaaactgt ttcaggttgt tttctggctg acataaacac cagcaataat gccagtcccg
     3001 cgacaatcgc ccccgtcacc ggcacaaagc tgtatcccag cccagcggaa attaccgcac
     3061 caccagcagc tgctcccagc gcatttccaa gattaaaggc accaatattg actgatgaag
     3121 acagacctgg cgcttcactg gcgacatgca tcacgcgcat ctgtaacgga ggtacaaccg
     3181 caaaggttgc tgcgccccac accaccatgc taatagctgc gccgaactca ttgcgggcca
     3241 ggaacgggat tgccagcata atcaccatca gcagcaacaa aaagcctttc aacgtgccgt
     3301 taactgaacg atctgccagt ttgccgccga gatagttacc gatagagaat ccgacaccaa
     3361 tcagcaccag cattgccgtg acgaacaccg gtgttgcgtg ggtaatactt tgcagtaccg
     3421 gagagatata ggtgtagagg gtaaacattg caccagctcc cagtaccgtc gtcagcaatg
     3481 cagacagcac ctgcggacgc attaataccg ccagctcttt tttcacttca ggtcgtgccc
     3541 ctgtaccacc tttaggtaat gagaagaaca gacttaccat tgaaatcact cccagccccg
     3601 ccgttgccag aaatgacatc cgccagccga tggtttcacc caaccaggtc gccgccggca
     3661 cgccaccgat atttgccagg gttaacccca taaacatagt ggcaactgcg ctggcctgtt
     3721 tatgttttgg caccacgctt gcggccacga ctgaacccaa accaaaaaat gctccgtgat
     3781 tcaggctggt caaaatacgt gaaagcatca gggtcatata atccggcgcg atggcggaaa
     3841 gaacgttgcc tagcgtgaaa attgccatca ggaaaatcaa cgcactgcgg cgggcacgat
     3901 gagaaagtag aagcgtcatc agcggcgcgc ccaccattac gccaactgca taggcactga
     3961 ttaacattcc ggcagcggga atcgagacat ccacaccgcg cgcaatgacg ggcaacaagc
     4021 ccattggcga gaactccgtt gtcccgatac caaacgcgcc aatcgccagc gccagcgcca
     4081 gcaacggata gttaattttc atgccttatc tccacctctt cgcgtcatta cgcgatattc
     4141 attaaagtgg cgaaagcatg acagcaatca caaaaaaatg aaaataacaa aaagagaaaa
     4201 cacttttgcc attttgctaa caaacaggaa ggagatgcga gggagaacgc gctccctcga
     4261 gaggaaatca gtgcagcgcg gcagtcaaac ccacggctac gatcaaaccg aggacgataa
     4321 ccgttgttac cagtgaaaat ttaaggtcgg tgctcatcaa gttttctcct tttttattac
     4381 cacacaaaaa gtgatattac gcatttttac acactgtgat gaaaaaatct cccgtcattt
     4441 ataatgataa gtgtttttac cacttcccct tttcgtcaag atcggccaaa attccacgct
     4501 tacactattt gcgtactggc cattgacccc ttcctgacgc tccgtgtcgt ttttccggcg
     4561 taccgcaaca cttttgttgt gcgtaaggtg tgtaaaggca aacgtttacc ttgcgatttt
     4621 gcaggagctg aagttagggt ctggagtgaa atggaatggc aacaataaaa gatgtagcga
     4681 aacgagcaaa cgtttccact acaactgtgt cacacgtgat caacaaaaca cgtttcgtcg
     4741 ctgaagaaac gcgcaacgcc gtgtgggcag cgattaaaga attacactac tcccctagcg
     4801 cggtggcgcg tagcctgaag gttaaccaca ccaagtctat cggtttgctg gcgaccagca
     4861 gcgaagcggc ctattttgcc gagatcattg aagcagttga aaaaaattgc ttccagaaag
     4921 gttacaccct gattctgggt aatgcgtgga acaatcttga gaaacagcgg gcttatctgt
     4981 cgatgatggc gcaaaaacgc gtcgatggtc tgctggtgat gtgttctgag tacccagagc
     5041 cgttgctggc gatgctggaa gagtatcgcc atatcccaat ggtggtgatg gactggggtg
     5101 aagcaaaagc tgacttcacc gatgcggtca ttgataacgc gttcgaaggc ggctacatgg
     5161 ccgggcgtta tctgattgaa cgcggtcacc gcgaaatcgg cgtcatcccc ggcccgctgg
     5221 aacgtaacac cggcgcaggc cgccttgccg gttttatgaa ggcgatggaa gaagcgatga
     5281 tcaaggtgcc ggaaagctgg attgtgcagg gtgactttga acctgaatcc ggttatcgcg
     5341 ccatgcagca aatcctgtcg cagccgcatc gccctactgc cgtcttctgt ggtggcgata
     5401 tcatggcaat gggcgcactt tgtgctgctg atgaaatggg cctgcgcgtc ccgcaggatg
     5461 tttcgctgat cggttatgat aacgtgcgca acgcgcgcta ttttacgccg gcgctgacca
     5521 cgatccatca gccaaaagat tcgctgggtg aaacagcgtt caacatgctg ttggatcgta
     5581 tcgtcaacaa acgtgaagaa ccgcagtcca ttgaagtgca tccgcgtttg attgaacgcc
     5641 gctccgtggc tgacggcccg ttccgcgact atcgtcgtta atcacccgtt gcgggagtct
     5701 cttccggctc ccgcagccac tccttattca gcgtctcact atcgctgaga tactcaagca
     5761 gccaggttaa cgcaggcgac atatcatttt gctgccatgt cagacaacat gccgaatccg
     5821 ggaaggggtt ttccagttca agtgctaccc acttcccctc attaagccac ggtttggcga
     5881 aatgtgttgg caccatccct atgcataatc ctgccgagat acaggttgcc gatgattccc
     5941 agtcaggtac gacaagtctt ttttggttat ccagcaacca ggtaatacgt ttaggtagcg
     6001 ttcgcgaggt gtcttcgcgc accaacgacg gccagttgcg caacgtatca tcgctgaacg
     6061 ggccatccat caacgccagc gggtggtgac tggcaacaac gcaactccag cttagcatcc
     6121 ccatatcccg gaaggcataa cgaccgccta ccggaatcgc gcgtgttgcg ccaattgcca
     6181 gttccacgcg cccgtcggaa agcgcatccc agacaccgtt gaacacttcc tgaaagacaa
     6241 gaagttcgac atcatcaaaa tggcgataaa aatcaacgat catctgccgt gtacgttctg
     6301 gcctgacaat attatccact gcgatagcta actgaccgcg ccagccgttc gctatctgct
     6361 gacattgctg gcgggtgatc tgcatttttt tgacaacaga gcgcccttct ttgagaaacc
     6421 acgctccagc agcggtcagc tccacatcac ggtgccgtcg ttcaaagagc ggtaccgcca
     6481 gccactcttc cagctgacgc acggtatagc tgaccgcaga aggaacacga tgcagctcct
     6541 gtgccgcagc gctaaaacta ccattacgcg ctaccgcatc aacaacttcg agtgaatatt
     6601 ctgaccacat agtctgcctg caaaattttt gaaaccagtc atcaaatatt accgtttcac
     6661 aacactaatt tcactcccta cactttgcgg cggtgtttaa ttgagagatt tagagaatat
     6721 acatgcaacc tgggaaaaga tttttagtct ggctggcggg tttgagcgta ctcggttttc
     6781 tggcaaccga tatgtatctg cctgctttcg ccgccataca ggccgacctg caaacgcctg
     6841 cgtctgctgt cagtgccagc cttagtctgt tccttgccgg ttttgccgca gcccagcttc
     6901 tgtgggggcc actctccgac cgttatggtc gtaaaccggt gttattaatc ggcctgacaa
     6961 tttttgcgtt aggtagtctg gggatgctgt gggtagaaaa cgccgccacg ctgctggtat
     7021 tgcgttttgt acaggctgtg ggggtctgcg ccgcggcggt tatctggcaa gcattagtga
     7081 cagattatta tccttcacag aaagtcaacc gtatttttgc gaccatcatg ccgctggtgg
     7141 gtctgtctcc ggcactggct cccctgttag gaagctggct gctggtccat ttttcctggc
     7201 aggcgatttt cgccaccctg tttgccatta ccgtggtgct gattctgcct attttctggc
     7261 tcaaacccac gacgaaggcc cgtaacaata gtcaggatgg tctgaccttt accgacctgc
     7321 tacgttctaa aacctatcgc ggcaacgtgc tgatatacgc agcctgttca gccagttttt
     7381 ttgcatggct gaccggttca ccgttcatcc ttagtgaaat gggctacagc ccggcagtta
     7441 ttggtttaag ttatgtcccg caaactatcg cgtttctgat tggtggttat ggctgtcgcg
     7501 ccgcactgca gaaatggcaa ggcaagcagt tattaccgtg gttgctggtg ctgtttgctg
     7561 tcagcgtcat tgcgacctgg gctgcgggct tcattagcca tgtgtcgctg gtcgaaatcc
     7621 tgatcccatt ctgtgtgatg gcgattgcca atggcgcgat ctaccctatt gttgtcgccc
     7681 aggcgctgcg tcccttccca catgcaaccg gtcgcgccgc agcgttgcag aacactctgc
     7741 aactgggtct gtgcttcctc gcaagtctgg tagtttcctg gcttattagt atcagcacgc
     7801 cattgctcac caccaccagc gtgatgttat caacagtagt gctggtcgcg ctgggttaca
     7861 tgatgcaacg ttgtgaagaa gttgactgcc cgaatcatgg caatgccgaa gtcgctcata
     7921 gcgaatcaca ctgatctata tcgatatact tatacttagg ctgctaacaa aattttgttg
     7981 tatgattgaa attagcggcc tatactaatt tcgagttgtt aaagctacga taaatattat
     8041 gtttttacgg ggacaggatc gttcccgact cactatggat ggtcatttcg gcaagggttc
     8101 ctcctttccc tctgttctac gtcggattat agactcgcgg ttttttctgc gagatttctc
     8161 acaaagccca aaaagcgtct acgctgtttt aaggttctga tcaccgacca gtgatggaga
     8221 aactatgagt tcatcgtgta tagaagaagt cagtgtaccg gatgacaact ggtaccgtat
     8281 cgccaacgaa ttacttagcc gtgccggtat agccattaac ggttctgccc cggcggatat
     8341 tcgtgtgaaa aaccccgatt tttttaaacg cgttctgcag gaaggctctt tggggttagg
     8401 cgaaagttat atggatggct ggtgggaatg tgaccgactg gatatgtttt ttagcaaagt
     8461 cttacgcgca ggtctcgaga accaactccc ccatcatttc aaagacacgc tgcgtattgc
     8521 cggcgctcgt ctcttcaatc tgcagagtaa aaaacgtgcc tggatagtcg gcaaggagca
     8581 ttacgatttg ggtaatgact tgttcagccg catgcttgat cccttcatgc aatattcctg
     8641 cgcttactgg aaagatgccg ataatctgga atctgcccag caggcgaagc tcaaaatgat
     8701 ttgtgaaaaa ttgcagttaa aaccagggat gcgcgtactg gatattggct gcggctgggg
     8761 cggactagcg cactacatgg catctaacta tgacgtaagc gtggtcggcg tcaccatttc
     8821 tgccgaacag caaaaaatgg ctcaggaacg ctgtaaaggc ctggatgtca ccattttgct
     8881 gcaagattat cgtgacctga acgaccagtt tgatcgtatt gtttctgtgg ggatgttcga
     8941 gcacgtcgga ccgaaaaatt acgataccta ttttgcggtg gtggatcgta atttgaaacc
     9001 ggaaggtata ttcctgctcc atactatcgg ttcgaaaaaa accgatctga atgttgatcc
     9061 ctggattaat aaatatattt ttccgaacgg ttgcctgccc tctgtacgcc agattgctca
     9121 atccagcgaa ccccactttg tgatggaaga ctggcataac ttcggtgctg attacgatac
     9181 tacgttgatg gcgtggtatg aacgattcct cgccgcatgg ccagaaattg cggataacta
     9241 cagtgaacgc tttaaacgga tgtttaccta ttatctgaat gcctgcgcag gtgctttccg
     9301 cgcccgtgat attcagctct ggcaggtggt gttctcacgc ggtgttgaaa acggccttcg
     9361 agtcgctcgc taaaggctat tctatcgccc cctcttcggg ggcgagttca gatcaggctt
     9421 ctgtacctgg ttgattcatg gcattttctc gtgccgccag cacacgttct accgtatcta
     9481 ccactgcctg agtttgtgga tcgatttcaa tgttgacgcg tgcgccaagt tttttcttcc
     9541 caagagtcgt gcgttccagt gtttccggaa ttaaatggac gcaaaaacgc gttggcgtga
     9601 cttcgccgac ggtcaggcta ataccgtcga tgccaataaa tcctttgtac aggatatatt
     9661 tcatcaactg actatcctgg actttaaacc agatctggcg attattttcc gaggttaata
     9721 ttttcgccac ttcagcggtg gtcataatat gacctgacat taagtgtccg ccaatttcat
     9781 cactgaattt cgccgcacgc tcaacgttta cccaatcccc cacttttaaa tcgccaagat
     9841 tggtaatgcg taacgtttct ttcatcaggt caaaactgac atggttgccg ttaatttccg
     9901 tcacggtcag gcagcaaccg ttatgcgcca cggaagcacc ggtttccagg ccgtccagca
     9961 tgtggtcggg taactccacc acatgcgtac gaaaatttgg tttctcgtca atcgacacca
    10021 gttttgcggt gccctgtaca atccccgtaa acatacttac aactcctgaa atcagttaag
    10081 acattctgtt cagcacaata gcaggtggaa aacgccctta ccagtgaagg ggtaagaatg
    10141 gctatttttt cactggagaa ttaataaatc ctcgctacaa tagactgaat ttcccctgct
    10201 tcttcttttt gctgcccatt caggcggctt tttagtctct catataacta caaataaaag
    10261 gtgttcacgt gcagaagtat atcagtgaag cgcgtctgtt attagcatta gcaatcccgg
    10321 tgattctcgc gcaaatcgcc caaactgcga tgggttttgt cgataccgtg atggcgggcg
    10381 gctatagtgc caccgacatg gcggcggtcg ctatcggtac ttctatctgg cttccggcga
    10441 tcctctttgg tcacggactg ctgctggcat taacgccggt tatcgcgcaa ttaaatggtt
    10501 ccggtcgacg tgagcgcatt gcgcatcagg tgcgacaagg tttctggctg gcaggttttg
    10561 tttccgttct cattatgctg gtgctgtgga atgcaggtta cattatccgc tacatggaaa
    10621 acatcgatcc ggctctggcg gacaaagccg tgggttatct gcgtgcgttg ttgtggggcg
    10681 cgccgggata tctgttcttc caggttgccc gtaaccaatg tgaaggtctg gcaaaaacca
    10741 agccgggtat ggtaatgggc tttatcggcc tgctggtgaa catcccggtg aactatatct
    10801 ttatttatgg tcatttcggt atgcctgagc tcggtggcgt tggttgtggc gtggctactg
    10861 cggcggtgta ttgggtcatg ttccttgcca tggtttctta cattaaacgc gcccgctcca
    10921 tgcgcgatat tcgtaacgaa aaaggcaccg ctaagcccga gcctgcggtt atgaaacgac
    10981 tgattcaact cggtttgccg attgcgctgg cactgttctt tgaagtgaca ctgtttgccg
    11041 tcgtggctct gttagtgtct ccgctcggta ttgttgatgt cgcaggacac cagattgccc
    11101 tgaactttag ttcactgatg tttgtgcttc caatgtcgct ggcggcagcg gtaactatcc
    11161 gcgtaggtta tcgtctgggt cagggctcaa cgctggatgc gcaaaccgct gcgcggaccg
    11221 ggcttatggt gggtgtctgt atggcaaccc tgacggccat tttcacggtt tcactgcggg
    11281 agcaaatcgc cctgttgtac aacgacaatc ccgaggttgt aacgctggct gcgcatttga
    11341 tgttgctggc ggcggtatat cagatttctg actcaatcca ggtgattggc agtgggattt
    11401 tgcgtggtta taaagatacg cgttccattt tctatatcac ctttacagct tactgggtgc
    11461 tgggcttgcc aagcggctat attctggcac tgaccgatct ggtcgttgaa cctatggggc
    11521 cagcaggctt ctggataggc tttattatcg gcctgacatc ggcagccatt atgatgatgc
    11581 tgcgtatgcg gttcctgcaa cgtatgccgt cagccatcat tctgcaacga gcgtcccgct
    11641 aataaagaca aggcgcaacc ttcacgggtt gcgcctgtgt ttttacgcag gctggagcgt
    11701 tgcgccaatc ccgtcttcgt ctggctgtaa tttcagagcg ttacacagag tttgccgact
    11761 ggcatctgtt atctcaacgc gtatatcccc gttaacttcc cctttcacat tgatgaagtc
    11821 aggtgaaatt tctttcgcac tggtttgcag gcaaatcgct ccctctccgc gcaggcgttt
    11881 cactttcagt tgcacatgcg ttgccgcacc agtgatcagc cacagttttc cgccattcag
    11941 attgatgtta ccaacaatga aagtgacgct gtcacctttt accgtactgc cgtcgagaat
    12001 caacacaccg ccctgctgta cagtaatcgt tgattgttgc agctctgcgc cataaccaat
    12061 aatcattgaa gcgccgtcca gtaatgcaac actcccggtc agttcaaccg gtgcgcgatc
    12121 ttcctctacg acaaaatttt cgtccgcgct ggtgggtgac aacaggatca ctgttccacc
    12181 ttgtattacc gcattttcca gcgtaccggc atgcacttca gcccgccccc ccacattcac
    12241 cgacaggttt tgcgtcttac cggaactgta gagctgaagg ccatccgtcc ccagacgata
    12301 aatggatcca ttctcaacaa tagtgtcaga gtcttcacca ccgagattat tcatggtgcc
    12361 accagcaagc aacgtatcac tggctttccc ggccagcata aaaacgccgc cctgattaat
    12421 ctcacaattg ctggcttctc cacttggata caccaacaat tcaccacctt catctaccac
    12481 gaccgctgag gttgtcccat taaccaacag gccgccctct tgatcgagaa tgattttttc
    12541 cgcgcgatgt ccttccagta cacgcaggtt accgccattt tccagcaaca aaccgcaggc
    12601 ataaccctga tcaacgctga attcaccttc gggatggcgg ccattaaccg tagcgagcgt
    12661 agagagacta attgcgcctc cctcggactg aacaatatct gtggcaatgg caccgtcgct
    12721 gaccgatagc gtgccattgt taccgtactg tgtgcatttg gcgatcccac cggttgccac
    12781 tttcaaccag ccagcccccg tgaccacggt attttcggct cttccgccag cttcaacaat
    12841 caactcgcct tcagtaagtt gcgtcgcgcc aaggacctcg ccggtcttaa caatttgcac
    12901 attcccgtcg ctcatcaagt ttttcgcatc agatcccatg atttattcct ttgctgcatc
    12961 tgtgtgcctt tattgctacc taagtgtaaa ggctacggag gatttatcca cgacagattt
    13021 gagatggtgg caaacaactc tgtttaaact ctgatacacg aattattggg ttgtatcaga
    13081 tgtaaatgcg atcctgaata aaaatcgccc ttgcaaatca acaaaatata gccaatttgc
    13141 ttaaaagctc tgcaaacggt cgaaatagcg cagaaaatta cgttttgcct cttgccacct
    13201 ttccactctg ccgctaatat tcgtccccgt tgtcacctac aacgttgcgt tcatagctca
    13261 gttggttaga gcaccacctt gacatggtgg gggtcgttgg ttcgagtcca attgaacgca
    13321 ccatcctgcg tccgtagctc agttggttag agcaccacct tgacatggtg ggggtcggtg
    13381 gttcgagtcc actcggacgc accagatttt cttaatctgg tcttttcctt tttccctctg
    13441 tttcttctct gtatccaata cgttaaaaga tttacactgt cttcgtatgc gttatcagaa
    13501 ggagaatcgc tatggcaact ttgttacaac ttcattttgc ttttaatggc ccatttggcg
    13561 acgcaatggc tgagcagctt aaaccacttg ctgagtcgat taatcaggaa cctggttttc
    13621 tgtggaaggt atggacagaa agtgaaaaga accacgaagc cggtgggatc taccttttca
    13681 ctgatgaaaa aagcgccctt gcctatctgg aaaaacatac tgcccgactg aaaaatctcg
    13741 gcgttgagga agttgtcgcc aaagttttcg atgtcaatga gccacttagt caaatcaatc
    13801 aggcaaaact cgcctgacag aatttaatca agggcggtta gcgccctttt catccctgtc
    13861 tgaaatttct caaattctaa aaatctcaac caaacttatc tgataacact aaattcgaaa
    13921 gaatgcgtac aggtaagtaa caatgaaaaa aattgctatt gtgggtgccg ggcctacggg
    13981 gatctacacc ttattctcgc ttctacagca acaaactcca ctttctattt ctatcttcga
    14041 gcaggctgac gaggccggtg tcgggatgcc atacagtgat gaggaaaact caaaaatgat
    14101 gctggcaaat attgccagta ttgaaatacc gccgattaat tgtacgtatc tcgaatggct
    14161 acaaaagcaa gaagccagcc atctccagcg ttatggcgtt aaaaaagaaa ccttgcacga
    14221 tcgtcagttt ttaccgcgaa ttctgctggg cgaatatttc cgcgatcaat ttttacgatt
    14281 agtagaccag gcacgacagc aaaaatttgc agtggctgtt tatgaatcat gccaggttac
    14341 cgatctgcaa attacaaatg ctggcgtcat gctcgctaca aatcaggatt tacccagcga
    14401 gacgtttgat ttagcggtga ttgccacggg tcacgtctgg cctgatgaag aagaagcaac
    14461 cagaacgtat tttcccagcc cgtggtcagg cctgatggaa gcaaaggtcg atgcgtgtaa
    14521 cgtgggtatt atgggaacat ccttgagcgg actggatgcg gcaatggcag tggctattca
    14581 gcatggttcg ttcattgaag atgataaaca acacgtcgtt tttcaccgcg ataacgcaag
    14641 tgaaaagcta aatattacgt tgatgtcgcg cacgggtatt ttacccgaag ccgatttcta
    14701 ttgccctatt ccctacgagc ccttacacat cgtcaccgat caggcattaa atgctgagat
    14761 tcaaaaaggt gaagagggcc ttttggatcg ggtatttaga ttgatagtag aggaaatcaa
    14821 gtttgctgat ccagactgga gccaacgcat agccttagag agcctgaatg tcgattcctt
    14881 tgctcaagcc tggtttgccg agcgcaaaca acgcgaccca tttgactggg cagaaaaaaa
    14941 tctccaggaa gtcgaacgca ataaacgaga aaaacatact gttccctggc gttatgtcat
    15001 tctgcgcctg catgaagccg tacaggaaat tgttcctcat ctgaatgaac acgaccataa
    15061 acggttcagt aaaggccttg ccagggtttt catcgataat tatgcggcaa tcccttcaga
    15121 gtctattcgt cgactactgg ccttacgtga agcggggatc tgaaatccct gatgttggcg
    15181 aagattatac gttacagcaa cccgaagata ttcgtggacg cgtagcgttc ggcgcgttgc
    15241 cctggttgat gcacgaccag cctttcgttc agggacttac ggcatgtgca gaaattggtg
    15301 aggcgatggc tcgggcggtc gtaaagcctg catcccgtgc tcgtcggcgt ctttcgtttg
    15361 attaaaggta aagctatcag cactcaacaa actgatgctg ttcattaaga cgatagcgga
    15421 ctccggttcg aatattgttt tcaccttcaa tggcgacggc gaagcgaact ctttcgccgt
    15481 catgatatgc cagtgcggcg cttcccccta gcccgagaat aattgagtcg accacgcccg
    15541 tgctcactac cgttgaattt tcaccgctgg caatgatgtg tacattgtcg ccgctgttgg
    15601 caattctggc gtttgcgcca aagctggcga tttgcaccag gtcaccatta ctggcgacat
    15661 gacaccgttc acccaatgta caaacgcgta ctcgcattcc ggtattcgca atccgactac
    15721 tatctccagc gctgcttatc cgcgaagaat taccggttac agcaacccgt gcgcgttcac
    15781 cggagctgcc aatgtgactg ttaaagccga cacttccaat ccgcacgcta taacccatac
    15841 tggcaatttg cgcggcatac ccagcggttg cgattcgggc gttatcctca caacagccga
    15901 tgcgagcatg atcgtcgctg cgggcaaact gactgcaaaa tggtatctct ccggggtctg
    15961 tggccagttt ttgcatagag gaaacttcct gatgcgcgaa gttttcatcc gccagccatt
    16021 ttttccacgc atactcaacc agactctccg cccagtccgt atatccttcc tcaatcagtg
    16081 catgacgaat atcggcataa ctaccgccgc gtgggaaatg gcgcaggaac cagcgataca
    16141 tcaccgctcc gatacgccat tctcgcaggt ctgctcgtgt aatgatcatt cgttagtgcc
    16201 ggtgatagcc atcgaccatg cttttaaagg tttcgtgtgg cgtacgcccc gtggtgcaaa
    16261 gataaagatg accgaagata aaaaagaggc ttataaatgc cagagcaaaa tgcgcctgca
    16321 ataaccagta tcttacgcca ggaaacacat ctcccacggc ttgcggatag agacacaaca
    16381 gccccgtcaa tagtaacaac ggcagcaatc catacatgac accaacatag gcgacctgct
    16441 gtaagggatt aaatttagac tgtgttgttg ccgggaaagg atgttcttcc ccctgcataa
    16501 tgccaaacaa ataaaatcgc gtttgttttg ccgctcgttc cagccacccc tgacgacgaa
    16561 tgcgatagtg gtgaccatta tccccaacgg cattgatcag cacaaagccg agccagcatg
    16621 ccagtaacaa aaatccgcaa acttcatgca ccgcaaccag acttttaacc gcagttgcgc
    16681 ccaccatcgc aaagtgattt atcagcccac ttgccagcaa caatacaaag agtaacgcat
    16741 tcgaccagtg ccataatctg accgctttgc tgtaaagata aaccttctca ccatgatcgg
    16801 tggctgattt cttcacgcca cgagcacgaa gcaaggcgtg cagccccagc accaaccaca
    16861 tcccaaccag caatactccg gcaatgatca accacaccgg ccaaaattcg ggagtgaact
    16921 gtggcacata gttcgctaac tggctctgaa actgttcagc atgttgcgac gggttcatac
    16981 attttccttt ttaatcaaat gttgaccgaa ccggcgatac agatgcggtt ttccggcacc
    17041 aggtagctga tattgatagt atttattatc ctgcaaccac gcctgaattt ctgggctatc
    17101 ttcgcgacca aagatcaatg cgtgttccgg acaggcgctg acgcaaatgg gcggaaagcc
    17161 ttttgccagt cgggactcag cgcagaaatc acatttatct gccactttag tgacagggtt
    17221 aaggtagcga accaggtacg gacaagcgcc aatgcaataa ctgcagccga tgcactgcga
    17281 tttttctacc cgcacgatcc cctgctcatc acgccacgat gcccccgtcg ggcatacgtc
    17341 gatgcacgga gcatcttcgc agtgctgaca tgactggcga aaaaagtgat actgcgtttc
    17401 gttgtcgtta tcggtgactg gaatgtgtgc tatcgataaa cggcttccct gagcggggac
    17461 gtggttagtt ttacggcatg cgcgggcgca aatattacag ccattgcacc gtgactcgtc
    17521 atgaatcatg gcataccgaa cttccttttc ttgcctcgtg ttcgctaaca gagaagacgc
    17581 agaaccagta aaaaagatta ctgttcccat ccccagaaca aatttgcgtc gagtgaagga
    17641 catctgatta tcccctaatg cagtggctgc aactgtccac cctatccatg acatctccct
    17701 gaaatttcag gggttctgaa actgctctac ctcgaaccat tcgcataagc cataagcctt
    17761 gccataggtc ataaatcgct cgctaaacaa gtacaacacc gccgtcgatg actccacctg
    17821 ccgtatatcg gcatattcag gtgcaacatc catcgcggcg atcgcggctt caatctgcgc
    17881 ttcctggaag taatatggcg cttgcatcag catggcgact ttgtaaggac gaggatacgt
    17941 ctggcactca aaacgaacgg cgtgtgcaat ggcgcgacaa atgtcctgct ccacaacttg
    18001 caaagacatc gccgcgtagt tttcactcat ggcctgcgtt gagtaatagt aatcatcctg
    18061 acttcccgtc atacaagcga tatccgcgta gtctccgttc tgcttaatgc tttccagcaa
    18121 aactgacagt tcctcttctg caaccgaata gggtggcgag agaaacacgg tgcgcgccac
    18181 cagctgccca gccgcagaat gctggcgaat gaactcagcc aggatttcag caggttctgg
    18241 ttcggttacc gtatcaacgg ggtgacgcat cccctgcaac cattgccgct tcgcctcgtc
    18301 aacttcgctg actttcgcta acggcaattc atcctgatgg ttcatcttac ccattcctta
    18361 cgcaggcagt agattgtgtg ctgccagatc ggcggcaata tcttccagcc ccagacgctg
    18421 caatgtttcg cgggttggac aaccaagctg tgggtcccag cccatttctt tgtagaacat
    18481 cgtgagcgaa gcatgcatat cgtcacgatc cattttgtca gtaccttcag taaagaccgg
    18541 gatctgcgga tccttgtcga aaacccagga acagataaga tcgtgttcat tacgcatatc
    18601 tttggtttgc atcggtttta ccgtgtaggc acgatgcaac gtaaagatac gctctgcagc
    18661 taaatctaat ttttcctgag tcatctcttc gccggtgatc gctttgaaga atttggcttc
    18721 aagcgccaga tcgccccggt aattacggct tttcagcggc gaaacggtca ttggccagac
    18781 ccagttgcac agagtgacgg cgttatgcaa acagacccgc aacagcgacc actttgcata
    18841 tttaattttt gcgtcattga ttggcgtgta gtttttggtt tcatcgtaag catcttcaga
    18901 accaaaaagt tctttcgcca cttcacgttg cagtttcaat ggcaagccgg aaccaataaa
    18961 gttgatatgg gtatgcgtca tgcagtcacg gttgaacata cacttaacaa tggaaccgac
    19021 ctgcgctgac gcttcattgg catggtgaac cggatagcca aacggcgacc agagtttatt
    19081 tttcgcgtag ccccagtact ctttacccaa attccagcgt tctgcgatgg catatgaacc
    19141 atcagccagg tgactcagct cacccacacg atgcgccaga cggtagtaaa aatctttaat
    19201 gaagttaacg tcacccgctt ccagttgatc ccagcgaatt tctgcatact cttcagctgg
    19261 cagaacacgc ttgaacacac ctttgctgta acaataagta aagtcgcgat gcaactgccc
    19321 gtagttacac cataggccgt agtcatcgaa caaattcaga ccaaccaggt taccaatcac
    19381 acgaccatcg tctttatctt caaaatcttt cggcccgttc gggaagatgg tggtatggac
    19441 aaagtttgct acacaagtgt taccacctgt gctgggcacg ccaaactctt tcacccgagg
    19501 aatattcatt tgggtcatac aacggatcgg gcaagagtgg cagccgctca tttttactgt
    19561 gtatttctct gccgccggtc ctaagtcaaa aacggattta taggtacgaa agccgaccgt
    19621 attctgattg cctggcggaa tttcacccgt ttcaatcgga ccaccttcag ccgcgcccca
    19681 aaacagccct ttacgtgctg tccagcgtga cttgggatct gaatactctg cccacgattg
    19741 tggcgtactt ggcacgacat ggttgttatt cgcaccaata agttcagtca tcatgtaatc
    19801 attgagacgc ttcatctcct gacgatcggc aatgttgacc cctttcgtcc cttcaaccgc
    19861 aatcgctttc aggtttttcg aacccattat tgcgccagtt cccgcaccgc cgctgtggtt
    19921 acggctattc aacatgccag agagaggaat aaggttttcc ccagcctgac caatagccgc
    19981 cacacaggtt tccgggctgg tcaatcgaca aatttcttcc gtcgttgcgc gcgtcccttt
    20041 tccccataag aaatcggctt tttccaggct aactttgtca tctttaatct tcagccatac
    20101 cggtgatttc gccttccctt cgataataat gacgtcgtat ccagcgaatt tcatttgcgc
    20161 tgcaaaaaag ccacccatat gggcatcgcg actaaatttc ctttggtaaa agtagaaagt
    20221 gaggtgatat ttacgcgaga actacagggg gcaccagatc caggtaatgg gccggtagca
    20281 aagactaatt tattcgcttc atcgaaaggt ttcgtgccta gcggtacttc gtcatacata
    20341 attttgtagc cgaagcccat gccaccgaca aaacttttaa acttactgga atcttcgagg
    20401 gtaatatttc ctgtcgtgag attgactctt aatatattac ctgtccaacc gttagccatg
    20461 attttttcct ttgcaagatt cacacagtaa tatctttcca ctcgataatt tttaacgccc
    20521 ctgttgggca ggcgtttgcg cattcaccgc ataacacaca tttcgaggat tttttacttt
    20581 cggtatttac ggtggccatc atccacgggc atgccgtagt acaggcgcta cagccaatac
    20641 aacgtttatg atcgacggta atacagcctt ctttctgctg ccaggtaatc gcaccaatcg
    20701 ggcagacgtt catgcattgc ggctctttgc attgacggca ggtgtccgcg gtatagttga
    20761 gatcgccata caggccgccg ccagagccaa ccccgttgtc gccaaagaaa taattgcgat
    20821 ggattttgat acgggagaag aatgtcccta ctgagccatc gttgaagttg gtacaagaga
    20881 tttcacagcg gtggcaaccc gtgcatcgcg ctcgctgggt caccagcacc cctttgggtg
    20941 tatttatcaa ccctactgtg ccgctatcaa tatcttcctg tttgcaaccc aaaagtgaca
    21001 gtaacgcagg ggcaattgtt agaccagcaa ggccttttcc tgatatacgc aagaattcta
    21061 atcgcgttaa gccaatatct aatagtggac gatcaaccgg gttcatttat ttcgcatcct
    21121 cttcgcaaac acagtgctga caattcagca gcctgatatt aagcattcaa ctaatacgct
    21181 gctgactctg tccccaacac cagatatttt ccctaacccg atagtgtatg agaaaagatt
    21241 taacgccgat aaactaatcc tttatggtta ggagaaaaaa taatgtgatt attctgagcc
    21301 cttaacattg atcgttatca attaaaatta caaagcaaga aattataagc gaactgatat
    21361 ttattatcat ttgaaataaa tttaacttaa gcgacaatca tttgtgacg
//
LOCUS       DADLTY010000064        21277 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.064, whole genome shotgun sequence.
ACCESSION   DADLTY010000064 DADLTY010000000
VERSION     DADLTY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.064"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..446)
                     /locus_tag="JN334_001985"
     CDS             complement(<1..446)
                     /locus_tag="JN334_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HAY7438131.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(501..1352)
                     /locus_tag="JN334_001986"
     CDS             complement(501..1352)
                     /locus_tag="JN334_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAY7438132.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1365..2165)
                     /locus_tag="JN334_001987"
     CDS             complement(1365..2165)
                     /locus_tag="JN334_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY7438133.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2228..3199)
                     /gene="manX"
                     /locus_tag="JN334_001988"
     CDS             complement(2228..3199)
                     /gene="manX"
                     /locus_tag="JN334_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAY7438134.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3662..5212
                     /gene="yoaE"
                     /locus_tag="JN334_001989"
     CDS             3662..5212
                     /gene="yoaE"
                     /locus_tag="JN334_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HAY7438135.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5216..6814)
                     /locus_tag="JN334_001990"
     CDS             complement(5216..6814)
                     /locus_tag="JN334_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7438136.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="JN334_001991"
     CDS             complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="JN334_001991"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY7438137.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8493..9071)
                     /locus_tag="JN334_001992"
     CDS             complement(8493..9071)
                     /locus_tag="JN334_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAY7438138.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="JN334_001993"
     CDS             complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="JN334_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HAY7438139.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10510..10689
                     /locus_tag="JN334_001994"
     CDS             10510..10689
                     /locus_tag="JN334_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HAY7438140.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10809..11168)
                     /locus_tag="JN334_001995"
     CDS             complement(10809..11168)
                     /locus_tag="JN334_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HAY7438141.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11530..11874)
                     /locus_tag="JN334_001996"
     CDS             complement(11530..11874)
                     /locus_tag="JN334_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY7438142.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12006..13916
                     /locus_tag="JN334_001997"
     CDS             12006..13916
                     /locus_tag="JN334_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAY7438143.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13974..14669
                     /gene="tsaB"
                     /locus_tag="JN334_001998"
     CDS             13974..14669
                     /gene="tsaB"
                     /locus_tag="JN334_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAY7438144.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14709..15290
                     /gene="yeaY"
                     /locus_tag="JN334_001999"
     CDS             14709..15290
                     /gene="yeaY"
                     /locus_tag="JN334_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HAY7438145.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15496..17181
                     /gene="fadD"
                     /locus_tag="JN334_002000"
     CDS             15496..17181
                     /gene="fadD"
                     /locus_tag="JN334_002000"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HAY7438146.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17263..18378
                     /gene="rnd"
                     /locus_tag="JN334_002001"
     CDS             17263..18378
                     /gene="rnd"
                     /locus_tag="JN334_002001"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HAY7438147.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18432..19397)
                     /gene="yeaX"
                     /locus_tag="JN334_002002"
     CDS             complement(18432..19397)
                     /gene="yeaX"
                     /locus_tag="JN334_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HAY7438148.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19453..20055)
                     /locus_tag="JN334_002003"
     CDS             complement(19453..20055)
                     /locus_tag="JN334_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-hydroxylating oxygenase subunit alpha"
                     /protein_id="HAY7438149.1"
                     /translation="MLEACPEVHNLKLAARFTTRTPANWKNIVDNYLECYHCGPAHLG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFHPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            complement(20412..20978)
                     /locus_tag="JN334_002004"
     CDS             complement(20412..20978)
                     /locus_tag="JN334_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7438150.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21190..>21277
                     /locus_tag="JN334_002005"
     CDS             21190..>21277
                     /locus_tag="JN334_002005"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7438151.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5265 a         5275 c         5466 g         5271 t
ORIGIN      
        1 acgagaagct tataaatttt ttccagatcg tcgatatttc gaacgcggat taacagccga
       61 cgctgttcta attgcatcac caacacgcca tcttccgaca agttcatcgc tttgattcgg
      121 ctatattcaa tccagacatt ggcgaagaaa aaaccttttt gtttaaagat gatcttcgga
      181 atgcggatcc agaatatata aaaacccatc agagccagtg cacttaataa ccatgttgtt
      241 attagcgcgc catgattcgt gacgttgtta tagataagaa tgacaatcaa tccgacgaag
      301 ataacgctat cgatgcgacc acgccggagc aaaggaattg ccagcagggt ggggccgtta
      361 cggcggggca tgatgaactg atcgtagatc gcgaaggcca ggagtgcggc gatgaaaaga
      421 atcagtacca ggtccgtgat tgtcattaat cctccagata aaaaaacggg gccaaaaggc
      481 cccggtagtg tacgacagtc ttacagtccc agcaggccgc aagcgtaacc agcgataccg
      541 atgacgaaga agccaacgat gatccacagc gggttaactt ttttgcgcag cagccacata
      601 caagcaaatg tcagcagcag tggtacaagg cctggcatta actggtcaag aatagtttgc
      661 acggtggtga cacgggtctg tccatcctgc ccggtgatgg tcgaaaccac cagcgggatg
      721 ttgacatgtg tccacttgtt aaccaatgcc cccatgacaa acaggccgag gatagacgcc
      781 ccttccgtca gtttttgcag gaagccacca cccatatctt taacgatatc gatacctttg
      841 gagtaaccat acgctacgcc gtagtaacgg gttgccagac gcaccaggtt aaacaggatg
      901 aagaacagca gcggacctaa caggctgccg ctcatcgcga taccggcacc cagtgctgca
      961 aataccggac gtacggttcc ccagaagatc gggtcgccta caccagccag cggccccatc
     1021 aaaccgactt tgataccgtt gatagcaccg tcatcgatct ctgcgccatt agcacgctgt
     1081 tcttccagcg ccagggttac gccgagaatc ggtgcagcca cgaacggctg ggtgttaaag
     1141 aactccaggt gacggcgaat agcttgttta cgagcttcgt tgttctcagg gtagaggcga
     1201 cgaattgccg gtaccataga gaagcagaaa cccagtgcct gcatacgttc gaagttccat
     1261 gaaccctgga agaggttaga acgcaggaat acgccacgaa tatcactttg agtgagtttt
     1321 ttctcggtgg tagtttgagt tgtatcaacc atttcgctca cctgttagtc cagttcgtta
     1381 tcgagatcgt tgttaccagc tgcctgagca ggcgcaccgg ctacgcggtt gtatttcggg
     1441 ctaagttgga tgtagagcac tgccataaca gtaccaatca cacccagagc aaccaggtta
     1501 aagttggtga atgctgcggt tacgaagccg aggtagaaga acggcatcag gtagccagca
     1561 cgcatcatgt tgataaccat cgcataacca accaccacga tcatgccccc ggcgatattc
     1621 agaccattgg tcaccacttc cggaatcgca ttcagcatgt tctgtacttc gctggtacca
     1681 acagacagcg caacgatgac ggccggaata gccacacgca ttgcttgcag gaacagagaa
     1741 gaaacgtgga tccaggaaat cgctgtcagg ttgccgttat cagcagcctt atccgcagcg
     1801 tgctggaaag caacggtaat agtacgaacg atgatggtca gtacctggcc cgcagcggcc
     1861 agagggattg ccagtgcgat acctgcacca atgctctgat gacctgcgat aaccagaatg
     1921 gtagaaatga tagaagccag agcggcgtca ggcgcaactg cagcaccgat gttcatccag
     1981 cccagcgcga tcatttccag cgtaccaccg ataataatac cggttttcat atccccaaga
     2041 acgataccca ccagggtaca cgcgattagc ggacggtgaa actgaaattc atcgaggatt
     2101 gatcccatac ctgcgataca ggctacgata aataccagca caatttgaag agtggtaatc
     2161 tccattgtac ttctcctgtt tacgtaagac ttaagtgtga aaactgaatg ataatcaaca
     2221 caatacgtta cttatcgatt ttgctgatca gatccatcat tttcagtttc ggatcggtgg
     2281 aaaccttacg gacttccagc tcaataccgc gtgcattcag ttttttgaac gcctcgatat
     2341 ctttttcatc aaccgaaacc gcgttattca cctgggtttt accctgacgg aatgccatac
     2401 caccgacgtt aacagaggtg attttcacgc cgccttcaac gagacgctct acatctgttg
     2461 ggttggtaaa taacagcatt acgcgttcgc cagcatattt cgggttgttg tagacgcgaa
     2521 tcattttggc aacatcaact acgtgtgctg ttacgcccgg aggtgcaacc tgggtgagca
     2581 gtgtcttacg aacggtatcc gcagccactt catcactaac aacaataata cgggagacat
     2641 tggtttcttt ggtccagcgg gtggcgacct gaccgtgaat cagacggtcg tcgatacgcg
     2701 caaggccaat aaccatgtag tcgtttggcc ccattggttt tgccggagtt ggagccgctt
     2761 ttggtgctgc ggcagcgggt gctggcgcgg ctttttcaac cggtttggct ttcagtgctt
     2821 tcacgccttc acggcctgtt tctactgcca gcgcaaccag ttcatcaaag ctcgggtcat
     2881 catcacgggc cattaacgtt tccacgagca ttggaatgtt aacgcctgca atgacttcat
     2941 aatgctcttt gtcgacgaca atgcggctgg cagcattgaa cgggctgcct ccccatgtat
     3001 caacgagaaa cagcacgcct ttagtggtgt cgagttttgc caactgagcg ttgtattttt
     3061 caatcagcgt ttcggcattt tcacctggaa cgaaatcgat ccagccgacg ttttcctgct
     3121 cgcctaacag catttctgcc gttttaagca actgctctgc agcccaacca tgtgtgccta
     3181 taacaatagc aatggtcact tgctacctcc tttattatcg ttaacacctc aacgtgccag
     3241 atgtattttt gaatcgtcgt ccacaatcga atcgattcag ataagggcta caaccaaaaa
     3301 cccctgcgtt tcgcgattta ttttagatat cgaaaaaatt attttatgtg atgaagatcc
     3361 gtaatttaac tttcgattat cagaaatttc gaaaggtaaa atatccttgt cacattcgtt
     3421 tgcaaaggaa ggtaaatctt tgccaaatca gaggcgtctc tgatatgttt aactcccgtt
     3481 taacaaccat ggagtatagg gcagtagccc gcagtatgga tcgtcaccga cgtcatttca
     3541 gcatcaggcc ttttaacgcc tgcctttctg gcactctatg ccgcaccttt cgtttgcatt
     3601 ttgtcgttac gcctgcatta tttctggcgt cgaatagcta ttccttaagc aggagcttgt
     3661 catggaattc ttaatggacc cctcaatttg ggcggggcta ctcacgcttg ttgttctcga
     3721 aattgtgctg ggtatcgata acctggtctt catcgccatt cttgctgaca aactgccgcc
     3781 aaaacaacgc gataaagcgc gtttgctggg gttatcactg gcgctgatta tgcgtctggg
     3841 gctgctgtcg ctgatttcat ggatggtcac gctgaccaaa ccgctattta ccgtcatgga
     3901 tttctccttc tccggacgcg atctgattat gttgttcggg gggatattct tgctgttcaa
     3961 agcaacaacc gaactgcatg aacggctgga aaaccgcgat catgattccg gccacggtaa
     4021 aggctacgcc agtttctggg tggtcgtcac acagattgtc atccttgacg ccgtcttctc
     4081 gttggatgcg gtaattactg cagtagggat ggttaaccat ctgccggtga tgatggcggc
     4141 ggtagtgatt gcgatggcgg ttatgttgct ggcatcgaaa ccgctgacgc gattcgttaa
     4201 ccagcacccc acggtggtgg tgctctgtct gagcttcctg ttaatgattg gtctgagtct
     4261 ggtggcagaa ggtttcggtt tccacattcc gaaaggttac ctgtatgccg cgattggctt
     4321 ctcgatcatt atcgaagtgt ttaaccagat tgcgcgtcgc aactttattc gtcaccagtc
     4381 gactttgccg ctgcgagcgc gtactgccga tgccatcctg cgtttgatgg gcgggaaacg
     4441 tcaggccaat gttcagcacg atgccgataa cccgatgccg atcccggaag gtgcatttgc
     4501 cgaagaagaa cggtacatga ttaacggcgt actgacgctg gcgtcgcgtt ctctgcgcgg
     4561 gatcatgacg ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat
     4621 ccgcgagcaa ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga
     4681 tgaaatcatc ggtatcgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga
     4741 tgtggcggcg attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat
     4801 caacctgctg ggcgtgctgc gtcgcgctcg cgggagcttt gttatcgtga ccaacgagtt
     4861 tggtgtggta caaggcttgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt
     4921 cccggacgct gacgaaacgc cggaaatcat taccgatggt aacggctggc tggtaaaagg
     4981 cggtacagat ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga
     5041 tgatatcgcg acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg
     5101 cgatgtgatt gatgtagggc cactgcatat caccatcttt gaagccaatg attatcgtgt
     5161 tgatctggtt cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcgttaac
     5221 gtaacgacat aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg
     5281 ccagccattt gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa
     5341 tatgcacgcc atgacggcgc aaatacttcg cctgttcttg cgtttccacg ccctccgcca
     5401 ccagttcaat attcagtctt tgccccagcg cgatgatgat atcggtcacc gtcgagttaa
     5461 ccgcgtcaga acctatagct gcggtaaatg acttatcaat tttcagcacg tcaggacgta
     5521 atgtttcaag ccaggaaaac gagctgttgc cggtgccgaa gtcatcaatc gccagtttga
     5581 cgtttttacg atgcagctcg cgggcaatcc gataatcaac atccagtaag gcatcgcgtt
     5641 cggtgatttc gaggatcagt tgctgaatcg ggtgagcgct aaaccagtac tgattgagat
     5701 cttttatcag cacaccacgg cgaaaatggc tgggtgcgac gttaatgcca acatgaaact
     5761 gactactcat cgggaaaaca tggtgctgac gaatggtttc tgccatcaca tagtgggtca
     5821 gtggcacaat taaatgatgt tcttccgcga taggaataaa cacatccggt gaaatccagc
     5881 cctgacgcgg attattccag cgcagcaaaa tctctacacc aatacattgc tggctgcgcg
     5941 cattaagcaa aggctgacag aacaattcga actcatgttg cgccaggccc agattaattt
     6001 cgcgggaaaa gctcatccgg taagcggtcg ccagccaggc gatatagccc accagtaaac
     6061 ttagcagcac cgccagcggt aattgtgttg gaagatagtg ccatgccagc gccgtagccc
     6121 ccggaccatt tacgttaatg gtaaaaggaa agctttgcga agaaacctga tagttttcat
     6181 tgtcttcagg ttgcggaagg gaatctacca gcccattacc atagagcaaa tgccgtttgt
     6241 ccaccgtcag gctggcgcta ctgatttgcg gcagttgtgg ctcaaggagc attgccgtca
     6301 gtaagtcgat gttaatcatc tctatgaccc cagcattgct actgcccgct gcaggcgtcc
     6361 attgaatcaa aaccggactg cctttaatca gggcacggtc gatcgtcagg cgtaaaagtg
     6421 gttgcggtgc aggaagttca gccagaatgt ccacgacggg cacattgcga taaccaaaaa
     6481 tgctggagca ataaagtgtg ccgtcttgca ccaggccaat ggatcgcaca gtttggagtt
     6541 ttgccgcctg tttacgtaat ggcaaatggg cgacagagca gggcagacca atcagcggaa
     6601 gcaagacttt gctacctgcc tgtaggggaa gcagtacttt atctaattcc tctacagcgt
     6661 gattggcgaa ttgtactacc cgttgttgat ttaagttacg ctgtgaaata aatcgcacgc
     6721 tcagggtcga agcgagcgta acgagtgcgc aaatcgtaca aacaatcatt cgattacggc
     6781 gataggtttt aatgatccgt tgtgcttttt gcatgcgggc caccctgtaa gcgtatggcg
     6841 acaaacgcct gacgcaacag tggaagtgta gtggggaata ttacagcaga cgagaaagcg
     6901 ggtataaatt cgcccatccg ttgcagatgg gcgagtaaga agtattagtc acactggact
     6961 ttgattgcca gaccaccgcg tgaggtttcg cggtatttgg cgttcatgtc cttaccggtt
     7021 tcgtacatcg tttcgatgac cttatccagc gagacgcgcg gtgcactggt gcggcgcaga
     7081 gccatccgtg cggcgttaat cgccttcaca gaggcaatgg cattacgctc aatgcacggc
     7141 acctgaacct gccctgcaac cgggtcgcag gttaaaccaa ggttgtgttc catgccaatt
     7201 tccgccgcca cgcaaacctg ttccgggcta ccgcccagca gttctgcaag acccgcagca
     7261 gccattgaac aggcaacacc cacttcgccc tggcaaccaa cttccgcacc ggaaatagag
     7321 gcgttcattt tatacaatgc accaatcgcg cccgctgcca taaagtaacg ggtatagatg
     7381 tctgggctga ccgattcaat aaagtggtca tagtaagcca gcactgccgg aacgataccg
     7441 caggcaccgt tggttggcgc agttaccaca cgaccaccgg cggcgttttc ttcgttaact
     7501 gccagcgcaa acatgtttac ccagtcaatg acattcatcg gatcgttaga cagtttatcg
     7561 ctggaaacta acatccggcg cagggcagac gcacgacgtg gcacgcgcag cgggcctggc
     7621 agtacacctt cggtgttcat cccgcgatcg atacatgcct gcatggtttg ccagacatgc
     7681 gcgaaatact cgtcgatctc tttcttgctg tgcagcgcca gttcgttctg catagcgaga
     7741 ccagacagcg aatagccggt ttcattacag tacgcgagca gttcggtggc agatttgaac
     7801 ggatacggca cgctcacttc gttggcagca tcctgaccaa agtgttcttc atcgacgata
     7861 aaaccgccgc cgatggaata ataagttttg ctgtagacga cttcatcgcc gttataggcg
     7921 tggatttgca taccgttttc atgcagcggc aggttgccgt tatgaaaacg catcccgttg
     7981 tcgcgcggga aatccacttc atgccgtccc tgtgccagca gcagacgttc gcgctctttt
     8041 acgtcgcgaa taaaaccggg aatactgtcg atatccacgg tggcaggttc gttacctgca
     8101 agacccataa taatggcgat atcggtgtgg tggcctttac ccgtcagcga cagtgaacca
     8161 taaacgtcca cggcaacgcg agtaacgcta tccagtaagc ctttttcgac cagatcatcg
     8221 acgaactgtt tacccgcctt cataggccct acggtatggg aagatgaggg accaatcccc
     8281 accttaaaca tgtcgaatag actaatcacg ataatactcc tgacaaggtg actggacttc
     8341 cagtaacgaa ctaataacag cgcatagtgt aagagggaac gcggcgactg gcttaactat
     8401 tcacatgaat taaactaatc aaaaaccgcg atttaactaa tattgcgatg attgtctcaa
     8461 gtagtgtttt gtagatgtaa agataagtat agtcaggggg tcacaccaat ttgcagcgcc
     8521 agctcacgaa ttatgcctgc ggtcattccc catacaaaat actgttcgta ccaggacagc
     8581 catacccgat gtgaatcgcc acggcggtag atatctaaag ggtgataacg acccagatgt
     8641 aatgcctggg cgagcggcat ttcaaacacc gccgagactt catcttcact ggcgcgatac
     8701 ggcagatcgg gcgggataat gccgaccact ggggttactt gatagccagt tacgctatcg
     8761 acgggcggca gcacgccgat aacttcaacg gcggaaggcg gtatagcgac ctcttcttca
     8821 gcttcgcgca gcgcggcggc gataactgat gcgtccgtgt catcgactgc acctccaggg
     8881 aatgccactt gtccagcgtg tttacgcaga tgaattgaac gctgagtcag caacaacccc
     8941 ggttgcggtc gacggacgat ggggattaac acggcagcct gacgatgatt taggggttcc
     9001 cggttaattt gcgggcgcaa aagttgaaag cgagataaaa aatcatcaag cgtcaggcta
     9061 cggtattcca cgtcttactt ctccagttgg cgtaatatct tattaacttt atcaaaagtt
     9121 tcctgatatt ccgcttcttc ctggctatcg gcgacaattc caccgcccgc agagcagtat
     9181 atttgtccgt taatggcagt cagcgtgcgg atagtaatgc tggtatccat gttgccgcaa
     9241 aagctcaaat agccaatgct gccgcaccag gcattacgtc gctgcggttc cagttcgtcg
     9301 ataatttcca tagcccgtac tttcggagcc ccggttattg agccaccagg aaaagctgcg
     9361 cgcagcagat cgctggcgtg taactgttct ggtagtcgcg ccgttatagt gctgaccaga
     9421 tgatgcacgg cagggaaggg ttccaccacg aagagctctg gtacttttac cgaaccggct
     9481 acggcaacac gaccgatatc attacgcatt aaatcgacaa tcatcagatt ttcggcacga
     9541 tctttcgctg agttcgccag tttttctgct tgtttgctat cttcctgagg atcgggcagg
     9601 cgtggtagcg tgcctttaat cgggcgagtc tggatttcac tattatcaca aagaataaac
     9661 cgctctggcg aaaggcttaa aattgcaccc tgttcaagac gtaaaaaagc gctaaatggc
     9721 gcgcggttgg cctgattaag ctgaaggaat gcctgccatt catcgccaga ataggtcgca
     9781 tgaaaacgct gggcgagatt cacctgatag caatcaccgc tgtgcagata ttcctgtacc
     9841 tggcgaaatt tttcgccgta ctgctcgtgg gtcatattgg attgccagtc ggaagtgagc
     9901 gtgaaatctt cctgcggcga gaattgctgg ctttccagcc aggcccgacg ggcattgaca
     9961 tcattatgac tcagcaaaga aactgtatga cgctggtggt cgacaatgag cgcccaatcg
    10021 tagataccca ctgccatatc cggcagaacg atatcttgtt ccgcaatttc tggcagtgac
    10081 tcaaaacggc ggcccagatc gtagccaaac aaccccagtg cgccgccctg aaatggcaaa
    10141 tcttcgttat gcgttgggcg aatgtctgcg cgatccagca cctgctggag cacctgtagc
    10201 gggtcatcag tggtcgttgt gcgtttttcg ctttcactaa caacggtttc tttacctaaa
    10261 gtggttaaag tgcaaatcgg atcggcgacc acaatatcaa agcggctata cggatgatcg
    10321 gcatagccgg agtgtaaaag catcgcccac ggcaggtggc ttaagcggga gaaataaaat
    10381 tcagcggcgt cctgacgcca gggtaaagta atcacagtgg gagataacgt cttcatcagt
    10441 cctgactcta ctggctatgt gccgggtagc atgaaataat tagcgccaac aatttagcag
    10501 gagttaacaa tgtttgcagg tttaccttca ctcatccatg aacagcagca aaaagctgtc
    10561 gagcggatcc aggaactgat ggcacaaggg atgagtagcg gccaggcaat cgcgctggtg
    10621 gcagaagagc tgcgtgccaa ccactccggc gagctaatcg tggcgcgttt tgaggatgaa
    10681 gacgagtaac acactaatcc gtatcgattt cgttatagaa gccgatgatc atttgattgt
    10741 gagaaaaagg acgcttttta tatgtgatta aaagcgtcct gacccattta tgctgatata
    10801 aatttaactc agaccagtgc ttttagttct tcctgcatat atgttgaaaa gtattcagga
    10861 tttttaatca cagagcgttc acctgtctcc attttttttg cccatccaac cattttttcc
    10921 gtgaacccag ggttccagcc ttcctggtca gctcgcgccg taatatcagc ggcactcgcg
    10981 ggtactccca gttcttttaa atatttaaag attcccttcg ccgtgctttc attcatcgaa
    11041 cttggtgttg gcgctgatac atccatggca ccaatgaaat ccagggcttt atctattaca
    11101 gcaggcatat tcttatcctt aaaattaacc atgttatcga taaggctata tatgacctta
    11161 atttgcatct aatatcaaag ggattataac gatatggcac gagttaacct gttgacattc
    11221 aggtgtttta aaggtagcag ctcatggtgt gttatccagt aagatggaga aaattaacgg
    11281 tcagcccgca cgctataacc agattgttca gctaagaaag aaaaccagag ttgctaatca
    11341 aagggcgaac acaacggcat ggttatgttt atttatatag ttagttttat agataaaatt
    11401 gaaattataa atataaataa tgaattagca gaagcatcaa cagtaatatc ttattctctg
    11461 gtcgatttat gtaaaaccga ccagtattat cgataaatta tcgtctgact tattttgctt
    11521 cgataaagct tacaccgcag ccacaatctt aatttcaact ttatacttcg ggttcatcaa
    11581 acccgcttgt accgtgcagc gcaccggcgc atgacccgcg acaacccagg catcccacgc
    11641 tttattcatc gccgcgaagt cgtttttatc ggccaggaaa atggtggcat ccagaatgct
    11701 cgatttattg ctgccctgtt tttccagcac ggcgtcaatc tgtgccagcg tgttggcggt
    11761 ttgctcaaag gcatcggcgt cgaggttttc cggtacacca gtgtagtaga gcgtgttgtt
    11821 gtggattact acatcagacc agcgggcttc agcatcgata cgaacgatag tcatcatttt
    11881 gtcctcatta tacttccatt ttcgtgctgc aaagactgcc acaatgtgag gttgccgtca
    11941 ccacttcaac tggcgaaagc gccctcatcc tgacataatc cctgttcaaa tcaacagggg
    12001 gtagtgtgac ggacgatttt gcaccagacg gtcagctggc gaaagcgata ccaggcttta
    12061 agccgcgaga accacagcga cagatggcgg tagccgtcac ccaggcgata gaaaaaggcc
    12121 agccgctggt ggtggaagca ggaaccggta cgggcaaaac ctacgcttac ctggctcctg
    12181 cgctgcgggc gaaaaagaaa gtcattatct cgaccggctc aaaagcgttg caggatcagc
    12241 tctacagccg cgatttgcca acagtctcaa aggcattgaa atatacgggc aacgtggcgc
    12301 tgctgaaagg gcgctcaaac tacctctgcc tcgaacgtct cgaacagcag gcgctggcgg
    12361 ggggcgatct gccggtacaa atcttaagcg atgtgatcct gctgcgctcc tggtctaatc
    12421 aaacagtcga tggtgatatc agcacctgcg tcagcgtggc ggaagattca caggcgtggc
    12481 cgctggtcac cagcaccaac gacaactgtc ttggcagcga ctgcccgatg tataaagatt
    12541 gctttgtggt caaagcacgt aaaaaagcga tggacgccga tgtggtggtg gtaaaccatc
    12601 atctctttct ggcggatatg gtggttaaag agagtggatt tggcgagttg atccctgaag
    12661 cggacgtcat gatcttcgac gaagcccacc agctaccgga cattgccagc cagtattttg
    12721 gtcagtcact ctccagtcga caactgctcg acctggcaaa agacatcacc atcgcctacc
    12781 gcaccgaatt aaaagacacc cagcagttac aaaagtgcgc tgatcgtctt gcccagagtg
    12841 cgcaggattt tcgtctgcaa ctcggtgagc caggttatcg cggtaacctg cgtgagctgt
    12901 tagctaatcc gcaaattcag cgggcatttt tactgctcga tgacaccttg gaactttgtt
    12961 atgatgtggc gaaactgtca ctggggcgtt ccgccttgct ggatgcggca tttgagcgcg
    13021 ccacgttgta tcgcacacgg ctgaagcggc taaaagagat caatcagccg ggctacagct
    13081 actggtacga atgcacttcg cgccatttta ctctggctct cacgccgctc agcgtggcgg
    13141 ataaattcaa agagttaatg gcgcaaaagc ccggtagctg gatcttcacc tcagcaacgc
    13201 tgtcggtgaa cgacgatctg catcatttca cctcgcggct tgggatagaa caggcggagt
    13261 cgttgctatt gccaagcccg tttgattaca gccgccaggc gttactctgt gtgccgcgca
    13321 atctgccgca aaccaaccag ccaggttctg ctcgccagtt agcggcaatg ctgcgaccga
    13381 tcatcgaagc taacaacggt cgttgtttta tgctttgtac ctcgcacgcc atgatgcgcg
    13441 atctggccga gcagttccgc gctaccatga cgcttcccgt tttgttgcag ggggaaacca
    13501 gtaaaggcca actgttgcag cagtttgtca gtgccggtaa tgcgcttctt gtggcaacca
    13561 gtagtttctg ggaaggggtg gacgtgcgtg gcgatacatt gtcattggta attatcgaca
    13621 aattgccgtt tacctcgccg gatgatccac tgttaaaagc gcgcatggaa gattgtcgtt
    13681 tgcgcggtgg cgacccgttc gatgaagtgc aactaccaga tgccgtcatt actctcaaac
    13741 agggggtagg tcgactgatt cgcgacgccg acgatcgtgg cgtgctggtg atttgtgact
    13801 atcggctggt gatgcgtcct tacggcgcga cgtttctcgc cagtctgccg cccgcgccac
    13861 gcacccgtga cattgcccgt gcggttcgtt tccttgcgat accatcctcc aggtaatttg
    13921 ttacggagta tggtatgatg cgcgcctttt tattgatcta agcacgaaga cccatgcgaa
    13981 ttctggctat cgataccgcg acagaggcct gctctgtcgc cctgtggaac gacggtactg
    14041 tcaacgctca ttttgagctt tgccctcgtg aacatactca acgaatctta ccgatggtgc
    14101 aggatatcct gaccaccagc ggaacttccc tgactgatat taacgctctg gcttacgggc
    14161 gcggccccgg tagctttacc ggcgtgcgca ttggtattgg catcgcgcaa gggctggcgc
    14221 ttggcgcgga attaccgatg attggcgtct ccacgctaat gacgatggcg caaggggcgt
    14281 ggcgcaaaaa cggcgcaaca cgcgtgctgt cagccattga tgcgcgaatg ggcgaagttt
    14341 actgggccga atatcagcgt gatgaaaacg gtatctggca cggtgaagaa accgaagccg
    14401 tactcaaacc cgaactcgtt catgaacgaa tgcaacagct ttccggcgaa tgggtgacgg
    14461 tagggacggg ctggcaagcc tggccggatc tcggtaaaga gagcgggctg gttttgcgcg
    14521 atggcgaagt gttactcccc gctgcagaag atatgctgcc gattgcttgt cagatgtttg
    14581 ccgagggtaa aacggtggcg gtggaacatg ccgagccggt ttatttgcgt aacaacgtcg
    14641 catggaagaa acttccgggc aaagaatgaa tcttaagagt atgccctgag aaaaaaagga
    14701 gtcgcatcat ggcggttcaa aagaatgtta tcaaaggcat actggcaggt acgtttgcgc
    14761 taatgctgag cggttgtgtc actgtgccgg acgccattaa aggcagcagt accacgccgc
    14821 aacaagattt agttcgggtg atgagtgcgc cgcagctgta cgttggtcag gaggcacgct
    14881 ttggtggcaa agtggttgcg gtacaaaacc agcaagggaa aacccgcctg gaaattgcta
    14941 ccgtaccgct ggacagcgga gctaggccga cgctgggaga accttctcgc ggtcgcattt
    15001 atgccgatgt gaacggtttt ctggacccgg tggatttccg tggacaactg gttacggtag
    15061 tcgggccaat caccggtgcg gttgacggca aaatcggcaa tacgccctat aaatttatgg
    15121 tgatgcaagt aacgggttac aaacgttggc atttaaccca gcaggtgatt atgccgcctc
    15181 agccgattga tccatggttt tatggcggtc gtggctggcc ctatggctac ggcggatggg
    15241 gctggtataa tcccggcccc gcgagagtac aaacagttgt aaccgaataa ttgcttgttt
    15301 ttaaagaatg cagaaacagc ggctggtccg ctgtttctgc attcttacgg taaagataaa
    15361 aataaatagt gacgcgcttc gcaacctttt cgttgggtaa ttatcaagct ggtatgatga
    15421 gttaatatta tgttaacggc gtgtatatca tttggggttg cgatgacgac gaacacgcat
    15481 tttagaggtg aagaattgaa gaaggtttgg cttaaccgtt atcccgcgga cgttccgacg
    15541 gagatcaacc ctgaccgtta tcaatctctg gtagatatgt ttgagcagtc ggtcgcgcgc
    15601 tacgccgatc agcctgcgtt tgtgaatatg ggggaggtga tgaccttccg caagctggaa
    15661 gaacgcagtc gcgcgtttgc cgcttatttg caacaagggt tggggctgaa gaaaggcgat
    15721 cgcgttgcgt tgatgatgcc taacttattg caatatccgg tggcgctatt tggcattttg
    15781 cgtgccggga taatcgtcgt aaacgttaac ccgttgtata ccccgcgtga gcttgagcat
    15841 cagcttaacg atagcggcgc atcggcgatt gttatcgtgt ctaactttgc ccacactctg
    15901 gaaaaagtgg ttgataaaac cgccgttcag cacgtaattc tgacccgtat gggcgatcag
    15961 ctatctacgg caaaaggcac ggtagtcaat ttcgttgtta aatacatcaa gcgcttggtg
    16021 ccgaaatacc atctgccaga tgccatttca tttcgtagcg cactgcataa cggctaccgg
    16081 atgcagtacg tcaaacccga actggtgccg gaagatttag ccttcctgca atacaccggc
    16141 ggcaccacag gtgtggcgaa aggcgcgatg ctgactcacc gcaatatgct ggcgaacctg
    16201 gaacaggtca acgccaccta tgggccgttg ctgcatccag gtaaagaact ggtagtaacg
    16261 gcactgccgc tgtatcacat ttttgccctg accattaact gcctgctgtt tatcgaactg
    16321 ggtgggcaga acctgcttat cactaacccg cgtgatattc cagggttggt aaaagagtta
    16381 gcgaaatatc cgtttaccgc tatcactggc gttaacacct tgttcaatgc gttgctgaac
    16441 aataaagagt tccagcagct ggatttctcc agtctgcatc tttccgcagg cggtgggatg
    16501 cctgttcagc aagtggtggc agaacgctgg gtgaaactga ccggacagta tctgctggaa
    16561 ggttatggcc tgaccgagtg cgcgccgctg gtgagcgtta acccgtatga tattgattat
    16621 catagcggta gcatcggttt gccggtgcca tcgacggaag ccaaactggt ggatgacgac
    16681 gacaacgaag taccaccggg tcaaccgggt gagctttgtg tcaaaggacc gcaggtgatg
    16741 ctgggttact ggcagcgtcc ggatgctaca gatgagatca tcaaaaatgg ctggttacac
    16801 accggcgaca tcgcggtgat ggatgaagaa ggattcctgc gcattgtcga tcgtaaaaaa
    16861 gacatgattt tggtttccgg ttttaacgtc tatcccaacg agattgaaga tgtcgtcatg
    16921 cagcatcctg gcgtacagga agtcgcggct gttggcgtac cttccggctc cagtggtgaa
    16981 gcggtgaaaa tcttcgtagt gaaaaaagat ccatcgctta ccgaagagtc actggtgact
    17041 ttttgccgcc gtcagctcac gggatacaaa gtaccgaagc tggtggagtt tcgtgatgag
    17101 ttaccgaaat ctaacgtcgg aaaaattttg cgacgagaat tacgtgacga agcgcgcggc
    17161 aaagtggaca ataaagcctg agcgttaagt cagtcgtcag acgccggtta atccggcgtt
    17221 ttttttgacg cccactaaag agaaaacaat ttgaattacc aaatgattac cacggacgat
    17281 gcgctggctt ctttgtgtga agccgtccgt gtctttccgg cgatagccct ggatactgaa
    17341 tttgttcgta cgcgcactta ttacccgcag ctggggttga ttcaactttt cgatggcgag
    17401 catctggcgc taattgatcc tctcgggatc accgactggt caccgctgaa agcgatcctg
    17461 cgcgatccgt ccatcacaaa atttctccat gcaggcagtg aagatctgga agtgttcctc
    17521 aatgtctttg gcgaattacc acaacccttg attgacacgc aaatccttgc tgccttctgc
    17581 ggacgcccga tgtcatgggg tttcgcttcc atggtggaag agtattccgg cgttacgctg
    17641 gacaagagtg aatcgcgcac cgactggctg gccagaccgc tgaccgaacg tcagtgtgaa
    17701 tacgcagcgg cggatgtctg gtatctgtta ccgatcaccg ccaagcttat ggtagaaacg
    17761 gaggcctccg gctggctacc tgcggcgctg gatgaatgcc gcctgatgca aatgcgtcgt
    17821 caggaagtcg ttgcgccgga agatgcctgg cgtgatatca ccaatgcctg gcagttacgc
    17881 acacgccaac tggcctgtct gcaactgtta gccgactggc gactgcgcaa ggcgcgagag
    17941 cgtgatctgg cggtgaactt tgtcgtgcgt gaagagcatt tgtggtcggt cgcgcgttat
    18001 atgccgggaa gtttaggcga actggacagc ctgggtttat ccggtagcga aatccgcttt
    18061 cacggtaaaa cgctgctggc gctggtggaa aaagcgcaga cattgccgga agatgcctta
    18121 ccgcagccga tgcttaacct gatggacatg cctggttatc gtaaagcgtt taaagcgatt
    18181 aagtcgctga ttactgacgt gagcgaaacg cataagatca gcgccgaatt gctggcatcg
    18241 cgtcggcaaa tcaaccaact gctgaactgg cactggaaac tgaaaccgca gaacaatttg
    18301 ccggagctga tttccggctg gcgtggtgag ctgatggcgg aagcattaca caatttattg
    18361 caggaatatc cgcagtaaaa tcttccgaag ccggactggg cgcgctcagc gccacatccg
    18421 gctttggcaa actacaaatc caacaccagg cgtttaccct tcgcacgcga acaacagatc
    18481 aacatacttt gctggctggc acgctcttca tcgctaaaat actgatcccg atggtcagct
    18541 tcaccttcca gtattgccgt ttcgcaggtt ccgcataccc cttcacgaca taaacattcc
    18601 actttcgcgg ctttattatt ttcaataacc tgcaaaatag tcatctcttc cggcaccaca
    18661 aactcttttc cggaacgggc aagcaccagg gtaaatgcat cgccggtttt gtcttcgata
    18721 gcaaattgct caaagtgcag tgtatcggcg gcgatgtcca gacgcgcagc ttcacttctt
    18781 accgcttcaa ttagcgcctc ggggccacag gtataaacgt gtgtgccagg ttcgatatcc
    18841 gccaataatc gcgctaattc cagtcgtgtt ccggttgatg aatgatgcaa atggactttc
    18901 tcagcctgcg gatgctggac tagctcagca cgaaatgcgc aactttctgg atttcgcgag
    18961 cagtaatgta gctgccagtc gatgtcgctg tgttgcaact ctgccatgtg cgacaggaaa
    19021 ggggtgatac caataccgcc cgcgataaac agatgctttc tggctgaggg aattagcgca
    19081 aacaggttat taggcgttga gatcgttaac cgatcgccca cttttacctg ctgatgcaaa
    19141 aagcgggaac cgccgcgcga gttttcttcc agtcgaacgg caatctgata actagaggtg
    19201 ttatgcggtg aactcagtag tgaatacgca ttgctgtact ggttatcacc atcgctcatc
    19261 tgcactagga tgtgacttcc tccggtgaac gcaggcagcg gtttgccatc ggttgccacc
    19321 agcgtgaagc gtttcacctg ttcggtaagg ggttcaacct ggctcacctg tacttcaaac
    19381 atttgatagt ctgacattgc tgtactccat caaaaatcag cccagcggcg gaaaataccg
    19441 ccgccgatgt cattagtcct taaacacctg cgccagcaga ttatggaaat gggcgatacc
    19501 atgttcggaa atgccactac cgctactgtc ggccatgatg cgcccctgac cacgatagcc
    19561 acgcgatttc agccctttct gtacgctttc aaccagacgt aaatcttccg gatggaacac
    19621 atcgcgatac cactcaatca gcgatttttg ctcgtcggtt aactcttcat tggtgaagta
    19681 aatatcgtag ttttgcaggg tagtttcaga atccaccggg aattcataaa tgaccgtcat
    19741 catccctttg atcggggtga cgttcagcat cgtgcacggc cacagccaga aaccgtggaa
    19801 tgccgcatcc gtaccctctt caaatttaaa cgactgttcg gacggtttgg cgaaaccgta
    19861 ttgcagcgtc cagttaccgt gcatggtgtg ccagtaacga tcaacctgta cggagtcgga
    19921 gaaacctaga tgcgccggac cacagtgata gcactcgaga tagttatcga caatgttctt
    19981 ccagttggca ggcgtgcggg tggtaaagcg ggccgctagt ttcagattgt ggacttccgg
    20041 gcaggcttcc agcactttcg cgcccagacc ggtgtattga ttcacttgaa gtacgaaaaa
    20101 aaaccgggag gacattggat tattcgggtt ctgatgggat tagatttggt ggggcttgca
    20161 agcctgtagc gcaaatttta gtcgttaatc aatgaaacgc gaaagatagt aaaaaattgc
    20221 ttttgtttca ttgaaaatac gaaaaacaaa aacactgcaa atcatttcaa taacagcttc
    20281 aaaaaacgtt caaaaccgat aacaaccagg ctgtaaccaa atgactcata tcacaaatca
    20341 gctcatgccg tttaggtatg ttacatgtgt gattatgtga ggtgaagtat gttttagctg
    20401 gttcatggtt gttatacggc tttttttaca tcttgtggtt cttgtgaagg agctacaaga
    20461 ccttcctgtt cataaatacc atactttgac aaaatctttt tacgagtatc tgttggtaat
    20521 gattcaagca ttttggctgt ttgtaaggtt tgctgcgaaa taccttgcaa atcagcctgg
    20581 atggaatcat cggaagaaaa aatctcaggc ataagagctt caaatccttt ctttctgaac
    20641 aactcaatcg cagaatgcag ttccttgtcc tctaacaatt cgacaattgc gatcagttta
    20701 gttttcaatg tgctgcggtc aggttgagaa tttttattct cttgattatt tttatgctca
    20761 accattggca tgctctgact ttggataaac cactctattg gacgccctgt tgcgtaggct
    20821 atttgtgcta atcgatctaa agacgggtat gtcttgccag acaggtagtt tctgataacc
    20881 ccatcagaaa gtccgcactt agctgcaaac gcccggcttg attctccatt cattgcctct
    20941 tcaaggcgtt ccttgaaacg cattatttcc gggggcataa aaatgcgctc tgcatttttg
    21001 ctttcttttt cacctgtcat gcccaatctc tctgttatat aaagaatttt acagaattct
    21061 ttgatttgca ctggttaaaa taaaaatgcg catagctttt gaattgagca tttttctgcg
    21121 ctatcgtttt tcccgatagc ggtagccggt atcagttacc gaaaacgtta agagagtaag
    21181 gatcgcagaa tgtcagcaaa aaaccaagac tggcacgcag agcagataaa ggctgctgtt
    21241 cgtatgaagg ggaagacact atctcaatta tccagag
//
LOCUS       DADLTY010000065        21264 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.065, whole genome shotgun sequence.
ACCESSION   DADLTY010000065 DADLTY010000000
VERSION     DADLTY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.065"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            15..923
                     /locus_tag="JN334_002006"
     CDS             15..923
                     /locus_tag="JN334_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438152.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATHKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
     gene            1162..1221
                     /locus_tag="JN334_002007"
     CDS             1162..1221
                     /locus_tag="JN334_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7438153.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1538..1597
                     /gene="ibsE"
                     /locus_tag="JN334_002008"
     CDS             1538..1597
                     /gene="ibsE"
                     /locus_tag="JN334_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HAY7438154.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1717..3150)
                     /gene="hldE"
                     /locus_tag="JN334_002009"
     CDS             complement(1717..3150)
                     /gene="hldE"
                     /locus_tag="JN334_002009"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HAY7438155.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3198..6038)
                     /gene="glnE"
                     /locus_tag="JN334_002010"
     CDS             complement(3198..6038)
                     /gene="glnE"
                     /locus_tag="JN334_002010"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAY7438156.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6061..7362)
                     /locus_tag="JN334_002011"
     CDS             complement(6061..7362)
                     /locus_tag="JN334_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HAY7438157.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7604..8224
                     /locus_tag="JN334_002012"
     CDS             7604..8224
                     /locus_tag="JN334_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAY7438158.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8288..9526
                     /gene="cca"
                     /locus_tag="JN334_002013"
     CDS             8288..9526
                     /gene="cca"
                     /locus_tag="JN334_002013"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HAY7438159.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9689..10510)
                     /gene="bacA"
                     /locus_tag="JN334_002014"
     CDS             complement(9689..10510)
                     /gene="bacA"
                     /locus_tag="JN334_002014"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY7438160.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10601..10969)
                     /gene="folB"
                     /locus_tag="JN334_002015"
     CDS             complement(10601..10969)
                     /gene="folB"
                     /locus_tag="JN334_002015"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HAY7438161.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11074..11691
                     /gene="plsY"
                     /locus_tag="JN334_002016"
     CDS             11074..11691
                     /gene="plsY"
                     /locus_tag="JN334_002016"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAY7438162.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11704..12635)
                     /gene="ttdR"
                     /locus_tag="JN334_002017"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(11704..12635)
                     /gene="ttdR"
                     /locus_tag="JN334_002017"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            12842..13685
                     /gene="ttdA"
                     /locus_tag="JN334_002018"
                     /pseudo
     CDS             12842..13685
                     /gene="ttdA"
                     /locus_tag="JN334_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13682..14287
                     /gene="ttdB"
                     /locus_tag="JN334_002019"
     CDS             13682..14287
                     /gene="ttdB"
                     /locus_tag="JN334_002019"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HAY7438163.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14335..15798
                     /locus_tag="JN334_002020"
     CDS             14335..15798
                     /locus_tag="JN334_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HAY7438164.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(15841..16854)
                     /gene="tsaD"
                     /locus_tag="JN334_002021"
     CDS             complement(15841..16854)
                     /gene="tsaD"
                     /locus_tag="JN334_002021"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAY7438165.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17092..17307
                     /gene="rpsU"
                     /locus_tag="JN334_002022"
     CDS             17092..17307
                     /gene="rpsU"
                     /locus_tag="JN334_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAY7438166.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17418..19163
                     /gene="dnaG"
                     /locus_tag="JN334_002023"
     CDS             17418..19163
                     /gene="dnaG"
                     /locus_tag="JN334_002023"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY7438167.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19358..21199
                     /gene="rpoD"
                     /locus_tag="JN334_002024"
     CDS             19358..21199
                     /gene="rpoD"
                     /locus_tag="JN334_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAY7438168.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5129 a         5878 c         5460 g         4797 t
ORIGIN      
        1 aacaagaagc gtttatgacg cttgagcagg agcagcaggt taaaacccgt actgccgaac
       61 agaatgcacg tattgcggct tttgaagctg aacgtcgtcg tgaagcagag cagacgcgaa
      121 ttctggctga acgacaaatt caggaaacgg agatcgagcg cgagcaggcc gtccgctcaa
      181 gaaaggttga ggctgaacgt gaagttcgta ttaaagagat cgaacagcag caggtcaccg
      241 aaatcgccaa ccagacgaaa tcgatcgcta ttgccgccaa atcggaacaa cagtcccagg
      301 cagaagcgcg tgctaacctt gcactcgcag aagcagtaag tgcgcaacaa aacgtagaaa
      361 ccactcgcca gaccgcagaa gccgatcgtg ctaaacaagt tgccctaatc gctgccgcgc
      421 aggatgcaga aaccaaagcg gttgaactga ccgtgcgggc gaaagcagaa aaagaagccg
      481 cagagatgca ggcggcggct atcgttgagt tagccgaagc tacacataaa aagggtctgg
      541 cggaagcaga agcacaacgt gcgctgaacg atgctatcaa cgtactttct gatgaacaaa
      601 ccagccttaa attcaaactg gccttgttgc aggcgctacc tgcggtaata gaaaaatccg
      661 ttgagccgat gaaatctatt gacggtatca agattattca ggtcgatggt ctgaatagtg
      721 gcagcgctgc gggtgatgca aacacgggta atgtgggggg cggaaaccta gcggaacaag
      781 cattatcagc cgctctctct taccgcacac aggctccgct gattgactcc ttgctcaatg
      841 aaattggcgt ttcaggcggt tcactggcgg cattgacttc acccttaatc tcaacaactc
      901 ccgtcgccga aaacgtaaaa taaattttca gctatgaacg tccctggcac aaaaagccgg
      961 ggatttttta tatctgcgtt ccgctaaaaa ctgcaaatga tcaggtcgtt gcaacgtttt
     1021 gcgtgaccgc tcggggaagg caaaactgcc tctgggaaag cgttgcgcga ggtccggcgc
     1081 tcatcaacaa tcggggagtc agcaaagggc tgaaacggga aagcccctcc cgaggaaggg
     1141 gccttacaga aggaaagggt tatgatgaag ctcgtcatca tactgattgt gttgttactc
     1201 gtaagtttcg cagcttatta acagccaatc agagggggga gaaatcctcc ctcacccttg
     1261 ttcctttact ttaggttgaa aaaaaatcag cgtcaagaag gtttcattaa cacatatccc
     1321 tggcccaaaa agccggggat tttttatatc tgcgttccgc taaaaggtgc aaatgctcag
     1381 gacgttgcag cgttttgcgt gaccgctcgg ggaaggcaaa actgcttcag ggaaagcgtt
     1441 gcgcggggtc cggcgctcat caacaatcgg ggagtcagca aggggctgaa acgggaaagc
     1501 ccctcccgag gaaggggcct tatagaagga aagggttatg atgaagttcg tcatcatact
     1561 ggttgtgttg ttactgttaa gtttcccgac ttactaacaa ctgatcagag gggggagaaa
     1621 tcctccctta cccttgttcc tttactttag gttgaaaaaa caacagcgtc aataggcctg
     1681 cctgctacga agcgagatct gtgaaccgct ttccggttag ccttttttat cctgttggat
     1741 cttcttgatg atgttagtgg tcgagcaacc gtcttcgaag ttgagcacca gcacttcgcc
     1801 gccgttggcc cagacttctt tactcccggc gatctcttct ggtttatagt cgccgccctt
     1861 caccagtaaa tctggcagga tcccggcgat caagcgctgc ggcgtgtctt cttcaaacga
     1921 caccacccag tcgaccgctt ccagtgcgcc cagcacaatc atacgctgtt cgagcgggtt
     1981 aacggggcga gaatcccctt tcagccgttt ggtggaggca tcgctgttca cagcgacaat
     2041 caagcggtca cccagtttgc gggcatttgc cagatagaag acgtgtccgg cgtgcaggat
     2101 gtcaaagacg ccattggtca tcaccacttt ttcaccgcgt ttacgcgctg ccgctacggc
     2161 cagcttcagt tcctcttcgg tcatcacgcc aaagccggta tctgcgcgcc cacgcactgc
     2221 attttccagc tcgatcggcg aaaccgtgga ggttcccagt ttgccgacca ccacgccagc
     2281 cgccgcattg gcaaagaagc aggcttcttc cagcgaatta cccgctgcca gcgttgccgc
     2341 caggacgcca atcaccgtgt cgcccgcacc ggtaacgtca tacacttcct gcgcctgggt
     2401 tggcatatgc agcggcgctt tacccggttg cagcagcgac ataccctgtt cggaacgggt
     2461 cactaacaga gccgagagtt cgtaatcggc aatcagtttc atgccgcgct caacaatctc
     2521 ttcttcggtc ttacatttac cgacaacagc ttcaaattcc gagagattcg gtgtcagcag
     2581 cgtagcgccg cggtagcgct caaaatcggt accttttgga tcaatcagca ccggaacgcc
     2641 cgctttacgc gccagttgga tcatctgctg tacgcttgcc agcgcacctt tggcgtagtc
     2701 agaaagcacc agcgcgccaa tcgaactcag cgcctgatta atccgctcgt gcagcggctg
     2761 cggatcaaca ccttcgaaac cttcttcaaa atccagacgg atcagctgtt ggttgcggga
     2821 aagtacccgt aatttggtga tggtcggatg cgtcggtaca gaaacgaagt cgcatttgac
     2881 gttgacgtcg gccagagatt tactcagcgc gcgcgctgca tcgtcaatgc ccgtcaaccc
     2941 gaccaggcgt gcattagcac cgagagaagc gatattcatc gccacgttag ccgcgccgcc
     3001 cggacgttct tcgatggtat tcactttaac cacgggcacc ggcgcttccg gcgagatacg
     3061 actggtgggg ccgtaccagt aacgatccag catcacatca ccaaccacca tcactcctgc
     3121 acgttcaaac tctggcagcg ttactttcat tcctgtctcc tgagagattc aaaatttgcg
     3181 cgcgataata ccatacttca ttcttccacc agccacttct gccagcttgc ccgcaccaga
     3241 tcacgctctg cggtgaagca atcctccgac acatggcccg gcaattcctg taatgccaga
     3301 tgatgaagtt catcgcgcag cgtcgtgtaa gcacgggtca gcgccatcgc ttcctgctct
     3361 tccataatgt cgttttgcgc cagcagctcc agaatacgca cgttgtctga ccagcgcgtt
     3421 aacttcggtt tttcatgagc gtagcgcaac accagatatt gggtaataaa ttcgatatcg
     3481 gtaattccgc cttcatcggc tttgatatca aagcgattac gatgtttatt gccgagatga
     3541 gcgcgcattt tctcgcgcat ttcccgcact tccgtttgca gagttttacc ttctcgcggc
     3601 agtgtcataa tctcacgacg caccgcgtca aagtgcgcag tgagctgcgg atcgccgtac
     3661 actacacgcg cacgcaccag cgcctgatgt tcccacgtcc aggcctcgtt tttctgataa
     3721 tcggcaaatg cttctgcgga tgtcaccagc attcccgccg ccccggacgg acgcagtcga
     3781 gcatccactt catacaaaat gccggaagag gtacgcgtac tgaacagatg cataatgcgt
     3841 tgcgccagac gcagataaaa ctgccgcccg tcgatttccc gctcgccgtc agtcatcgca
     3901 tccattgggc aatcatggag gaagataagg tcaagatcgg aactgtagcc taactcccag
     3961 ccgcccagct tgccgtagcc gaccaccgca aaaccgcgcc cttcacgttc gttgaggtga
     4021 tttggcttac cgtagcgggc caccatttga ccccacgcct gctgaacgac ggcatctatc
     4081 atagcttctg ccagccaggt taagtgatcg ctcactttca ttaccggtag cgtaccggcg
     4141 atatccgctg ccgcaatgcg taacagctgc gcctgtttga actgacgcag cgcctcaagc
     4201 tgttgttctt cgtcatcttc cggcacgcgc agcaaatact ggcgcagttc atcacggtag
     4261 gcatcggtcg ccgtcggctg ataaagggta tttggatcga gcaattcatc cagcaataat
     4321 ggataacgcg ctagctggct ggcaatcatc ggcgatgcgg cacacagaga aatcaaatgt
     4381 ttaagcgccg cagggaattc actgagcaat tctaaatagg ttgtgcgggt aacaatcccc
     4441 accagcaagg cggtaatgcg cgacagcgtg acagcagcgt cttcacgcgc acagacatca
     4501 cttagcagat gtggcatcag atggtcgagc acctgacgtc ctcgcggccc gatagtacgc
     4561 ttatccagct ctttgcggaa atcagcaatc atcgttagga cctgtttgcg atcatcctcg
     4621 ctaagatgcg ccagcactgg cgtagtgtca tcttcctgca acgcatcctg ccacagctca
     4681 cgccactgtt ccgacagcga ctcttcctga gtttcacttt cgtcgtcgcc aatcaattca
     4741 ttaaacaccc ggcgcacatt ggtcatatgt gcggtcagcg ccccggtcag ttgcggccag
     4801 tcagcaaagt ccatcgccca cgccagccgc gcacggttaa gctcatcaga aggaagcgtt
     4861 tgggtttgtt cgtcgttaat gctttgcagc aggttttcca gacgccgcag gaacagatat
     4921 gccactcgca attgttcagc atcgttttca gaaagcagat gcagcgcggc aatggcgctg
     4981 agcgttggca gtaaagagcg cgactgcagc aacggttcgc gcccaccgcg aatgagctgg
     5041 aacacctgaa cgataaattc aatttcgcga atgccgcctg cgccgagttt gatattgtcg
     5101 gtcaaaccac gtcgacgcac ttcacgggca atcatccctt tcatgttgcg cagcgactga
     5161 atcacgctga aatcgatgta acgacggaaa acaaacgggc gcagcatcgc acgcaactcg
     5221 ttagcataga cgccttcgct atcgcccata atccgcgctt tgaccatcgc gtaacgctcc
     5281 cagtcgcgtc cctgctcctg gtaataatcc tccaacgcgg caaagctcag caccagcggg
     5341 ccactttcgc caaacggacg cagtcgcata tccacgcgat agacgaagcc atcctgcgtt
     5401 ggttgatcca gcactttaat cagccgctgc cccatgcggg taaaaaactg cgcgttatcc
     5461 agttcccggc gtccgccctg cgtacaacca tgttccggcc aggcaaaaat cagatcgata
     5521 tcagaggaga aattcagttc cccgccgccc agcttaccca tgcctaagat cagcagcggt
     5581 tgcgcttcgc cctgcgcatt gcacggcgtt ccccactcgc ggcagcaggc gtcatacagc
     5641 cagtcacgcg ccgcaacaat cagcgtctcc gccagataac tgagctgctg caatatgctc
     5701 tcttcagtaa ccagtgccag cgtttgcgcc catgcgatgc gcaccataat gcgccgccgg
     5761 aatagccgca gctcgcgcat taacccggct tcgtcgctca cattactgag cgcctcctgc
     5821 aaccacacgc tgtaatgttg ccattcgtca gcctgtggcg gttggctttc cagttccgtc
     5881 agccactctg gatgcgcaat cacgctgtcc tgcacaaaat cactgaaagt aagtactgac
     5941 ttcgcctgtg cactaagtga ttcctcggct aaaagctctg gcagccgctc aacaacggtc
     6001 tgccagtact gctgtaacgg tgaagagagc ggcttcataa gcgattttat ccttgcctga
     6061 ttaacgtttt ccgctgtgca accagaacgg ttcctgattg tttgcctcat tacggaaatg
     6121 ttcaatttca atgcgttgcc cggtcgcaat agcgtgacgc agcccctgcc agttctccag
     6181 ccaggcttgc gcgacgacag gatcatagta acccgccagc aacagtattg agtcaatatc
     6241 acgcatcagg cgtggcaact ggtcacggta gcgatcgcct aacggctggc agaaaacgct
     6301 tttcagttca gcggcatggc gggaaagatg gatatcggca aagcgtttga aggagtcgct
     6361 aattttgccc tgcgctttgg catctaaaaa cggctgccat gctttgctta ccagccattc
     6421 ggtcaacgcc agcttcgcca ttgccgtttc ggtagagtag accgccgtca cggcagaaac
     6481 cgcagaagcg atggtcgctt cgcattgagt cagcaaatca cgtaagtgag tactcgcttt
     6541 acgcggcaca atgccaccga acagcatcag tgtatgacgg actaggctaa tggctgccag
     6601 cacctgctct ttcgccgcat cgttgccgcg tacccacagt tcttcatgat actgccattg
     6661 cgctaacacc agctcgagcg ccgcttccag cccctgttcc acatcggctt ttgccgcaac
     6721 atgcaaaatg gtggtcggtt tgatttcacg cgccggattg ccctgcgcca gatgatagcc
     6781 acgcgccgct ttgctcaggc tgccctggcg taatccggtt tgcgatacca gttggttcgc
     6841 cagtttcagc accgcgcgcg tgtcgccgct aagcagctcc agttccagct cgcagatagg
     6901 ctcagcaaat tcacccgctt tcacttcccc ctggtcgaga gcgatttcaa ttcggctgcc
     6961 atcgatctct actagccatt tttcgcgata aaaatcggtg ctaaacagcg actgcacgcg
     7021 ggaagcgaga tcggcaggca attcgccgtt cggccagatt tccgtcggta actgcgccag
     7081 gtcgagcgtc ggttcgctca acgccacgtt atattccggg cgctggtgta agccgcctgt
     7141 cactcttcct gcaactttca tggtcatctc atagcgaccg ttttcgccac gaatacgtaa
     7201 gcccatatcg tgcctacgca gccagttatc cggcgtttcg tagtaaatat tcagcaactg
     7261 cacggggtca tggtgctcgc cgcccagcgt attgagatgg tcacgcaacg cctcaacggc
     7321 actgtgatta acaataaact ttaattcgat ttcctgagcc atggccttta cttatcggtt
     7381 atgtcacata agtgacgatg aacggcgaac ttaatgcgat cttttttgtc agtagatagt
     7441 attttgcgcc aaattgccat gcaacgggca atttgacggg cgtaaaagtt tgaagcagtg
     7501 gcaagacctg acacagagga ttccgattga tgtcgaatgc tttgcgtagc gacactgatg
     7561 ccactactat cgttccactt tccatgacaa taacgacagc ctgatgccaa aattacgcct
     7621 gatcggatta actttactcg cacttagcgc gactgccgtc tcacacgctg aagaaacgcg
     7681 ctatgtttct gacgaactga atacctgggt ccgtagcggt ccgggagatc attatcgcct
     7741 cgtgggcacg gttaacgccg gcgaggaagt gaccttatta caaactgacg ccaacaccaa
     7801 ttatgcccag gtgaaagaca gctctggccg taccgcctgg atcccgttga aacaacttag
     7861 cactgagcca agcctgcgct cccgtgtgcc agatctggaa aatcaggtca aaaccctgac
     7921 cgataaactc accaatatcg ataacacctg gaatcagcgc accgcagaaa tgcagcaaaa
     7981 agtggcgcag agcgacagcg tgatcaacgg gttaaaagaa gaaaatcaga aactgaaaaa
     8041 cgagctgatt gtcgcgcaga aaaaggtcga tgccgccagc gtacagctgg atgacaaaca
     8101 gcgcaccatc atcatgcaat ggtttatgta tggcggtggc gtgctggggc ttggcttgct
     8161 gctcggtctg gtactgccgc acctgatccc aagccgcaaa cgcaaagatc gctggatgaa
     8221 ctaaatcgcc ttcttcgttg cactgtctta ttattaggaa ttgtgtattt gacgaaggga
     8281 tgatggcgtg aagatttatc tggtcggtgg tgctgttcgg gatgcattgt taggactatc
     8341 ggtcaaagac agagattggg tggtggtcgg cagtacgcca caggagatgc tcgacgcggg
     8401 ctaccagcag gtaggccgcg attttcctgt gtttctgcat ccgcaaacgc atgaagagta
     8461 tgcgctggca cgtaccgaac ggaaatccgg ttccggttac accggtttta cttgctatac
     8521 cgcaccggat gtcacgctgg aagatgatct taagcgtcgc gatctgacca ttaatgcgct
     8581 ggcccaggac gataacggcg agattatcga cccgtacaac ggtctgggcg atctgcaaaa
     8641 tcgtctgttg cgccatgttt cccccgcttt tggcgaagat ccgttacgcg tactgcgcgt
     8701 ggcgcgtttt gctgcgcgtt atgcccacct cggttttcgt attgccgatg aaactctgac
     8761 gctgatgcgc gagatgaccc atgcgggtga actggaacac ctgacgcctg aacgggtatg
     8821 gaaagagacg gaaagcgccc tcaccacccg caatccccag gtgttcttcc aggtactgcg
     8881 cgattgcggc gcacttcgcg ttttattccc ggaaattgac gcactgtttg gcgttccggc
     8941 acctgccagg tggcatccgg aaatcgacac gggtattcat accttaatga cgctctcaat
     9001 ggcggcgatg ctgagtccgc aggttgatgt ccgtttcgca actttatgcc acgatctcgg
     9061 taaaggactg acgccgccag aactttggcc gcgtcatcat gggcatggcc cggcgggtgt
     9121 taagttagtg gaacaactat gccagcgtct gcgcgtgcca aatgaaattc gcgatttagc
     9181 cagactggtg gctgagtttc acgatctcat ccgcaccttc ccaatgctga acccaaaaac
     9241 catcgtcaaa ttatttgatt ccatcgatgc ctggcgtaaa ccgcagcgtg tcgagcaact
     9301 ggcgctgacc agcgaggctg acgtgcgcgg cagaaccggt tttgaatcag cggactaccc
     9361 gcaaggccgt tggttgcgcg aaggctggga agtggcgcag tcagtgccga caaaagcggt
     9421 cgttgaagcg ggatttaaag gcgtggagat tcgcgaggag ctgacccgac ggcggattgc
     9481 ggcggtagcc agctggaagg aacaacgttg cccaaagcct gactgagtat aaaactaaac
     9541 gttagcacga accgtcccct cgccccttcg gggagagggt tagggtgagg ggaacagacc
     9601 agcaccggtg cgaacaatca cccccacccc ggccctctcc ctacagagcg agggagaaaa
     9661 cccggcctgc tcccgaacct tttctcaatc aaaagaacac gacatacacc gcagccgcca
     9721 caataaagcg ataaatggcg aacgggataa acgaaatgcg cttaatcaat tgcaggaagg
     9781 ttttaatcgc tatcagcgcc accacaaaag cggtgataaa cccaacggca aacatcggga
     9841 tatcgccggt tgtcaggaag ccccagcttt tgtagagatc gagcgccgtt gcgcccatca
     9901 tcatcggtac cgccagcagg aacgagaact cggaagcagc gtaacggctc acgcccatca
     9961 gcatcccacc tgaaatggtc gccccggaac gggagaaacc cggccacagc gccagacact
    10021 ggaaacagcc aatcataaac gcctgacgat aggtcatatc atcaagaccc ggcgcacgcg
    10081 gctctttcgg cttcaggcat tcggcggcaa tcagcaacaa accgccaacg accagcgcat
    10141 acatcacatt tatcgggtta aacaatgact taatcgtgtc gtggaataat agccccagta
    10201 ccaccgccgg aatcatcccc agcaaaatgt ggatcagcgt taaacgacct ttgctttcac
    10261 cttcgtgctg caacgggcgg ccaaagtgga tgccaatcag gccaaacaga cgccgccaga
    10321 acatcactac taccgccaga attgatccta actggatcac aacttcaaag gttttcgccg
    10381 tgtcgccctc aaaccccaac aagtgaccga caataatcat atggcccgtg ctggataccg
    10441 gcagaaattc tgtcaatcct tcgaccacac ccaatattgc cgctatcagc agcgagtgca
    10501 tatcgctcat caataaaccc ctaaattatt aaaatgtacc gcttgtccga actactgcag
    10561 tatgaccagg ttataaccgt ttggtttaac agctgtaaaa ttaattattt tctttcagat
    10621 tattgccacg ctcaatgatt acgccaacat tcgccgcccg cgccactgcg cctggcttgc
    10681 tgagtttgat acgcacccac ggcgagttga agcgtgctaa cagcagctcc gccacctctt
    10741 cagccacgcg ttccaccagc gcaaaacgcg ccccctcgac gtggctgacc accgtttctg
    10801 caatgtcagc gtaactgagg caatccgcca catcatcact tttcgccgct ttacggttat
    10861 cccatgccat ttcgatatcg aacactaact tctgttcgat ggtctgttcc cagtcgtaaa
    10921 caccaatagt ggtgattacc gaaagttgct ctataaatac aatatccatc acgtcctgcc
    10981 tgctttttgg ctaaccggat accacttccg gcgaaatgtg cgtattatcc acagattcat
    11041 cgttgaacac gaattttcaa aacggaacag cttatgagtg caatcgcgcc tggaatgatc
    11101 ctcatcgcgt acctctgcgg ctccatttcc agtgccattc tggtttgccg cttgtgtggg
    11161 ctgcccgatc cgcgaaccag cggctccggc aatccaggcg caaccaatgt gttacgtatc
    11221 ggtggcaagg gagcagccgt agcagtactg attttcgacg ttctgaaagg aatgttgcct
    11281 gtctggggcg cgtatgaatt aggtgtcagc cccttctggc taggcttaat tgccatcgcc
    11341 gcctgtcttg gacacatctg gcccgttttc ttcggtttta aaggaggaaa aggcgttgct
    11401 accgcttttg gagcaatcgc accaattagc tgggatctca ccggagtaat ggcgggaacc
    11461 tggttactga ccgtgctatt gagcggatac tcgtcgctgg gagcgattgt cagtgcactg
    11521 attgctccgt tttatgtctg gtggtttaag ccacaattca ccttcccggt ttcgatgctc
    11581 tcttgcctga tcctgctgcg tcatcatgac aacatccagc gtctgtggcg tcgtcaggag
    11641 acaaaaatct ggacgaaatt caaaagaaag cgcgaaaagg atcccgagtg atttctggtg
    11701 gatctacatg acctgatagc cttcatcggg cttgcccagc cgttgctggc accatgccgc
    11761 cagaaattcc gcgcagacac gaatttcatg ctgcgataga gcagctcccg gtaaacagcc
    11821 cagatattgg cgctttgtgc atactccggc aatacttgca ccagtttgcc actctccaga
    11881 aacggcaaca catcccactc ggaacgcagc ataatccctt tgccctccag cgcccattgc
    11941 aacactatct cgccgctatt ggaggaaaga tgcccgctaa ccttcaccga ttttttctcc
    12001 tgaccgttcc ccaactccca tattccgtgg gtcatatcgc gctctttggt caccagacag
    12061 tcatgacgac ttaattcctg taatgattgt ggctgcggat atttttgcag atactcaggt
    12121 gctgcgcata atattctttt atttttcgtt aacagatgcg caatataata atcaggaatt
    12181 tcgtcattaa tacgaatatc cagatcaata ttatcctgca ctaaatcaat ttgccgatcg
    12241 aacaattcaa aatgcacctg taactcagga taattgcgca taagttcggt aatggctggc
    12301 gcaatatggc tgcgcccaaa accaaaactg caaccaatac gaatcatccc ctccgggcgc
    12361 gttttgattt gcgtgacgtc atcgaccagt cgctgatatt gggtgaggat ttccagcgca
    12421 tgttcgtaac aacgttggcc actttcagtc agcgccacgc cgcgagcgga gcggttcagc
    12481 agcgttgttg ccagcgtatt ttcaaggatt tggatccgct tggtgacaaa tgcaggcgtt
    12541 tgcccaagcg tcgccgctgc agcactaaaa ctcccgctgt ggacgatttc taccagtacc
    12601 tgcaagtctt tggctaaagg ccagctattc agcatcgtta attatccgca gttgtgataa
    12661 gcgcagtgta tttcgcaaaa cattgccatt ccctgctctg gcgcaatatt cattcttatt
    12721 ttcgcgttga ttcacgaact gttaattaca tattaacggt ggcaaaaaat ataaaaccac
    12781 atttttagtg ttagtttgtg gcggtgaatt tttccagaca aatacaaaaa ctggagttgc
    12841 catgatgagc gaaagtaata agcaacaggc agtgaataag ttgacagaga ttgtcgctaa
    12901 ctttaccgcc atgatttcta cccgaatgcc tgatgacgtg gtggataaac taaaacagct
    12961 aaaggatgcc gaaacgtcgt cgatggggaa aattatctac catacgatgt tcgacaacat
    13021 gcaaaaagcg attgacctga atcgtcctgc ctgtcaggac accggggaga ttatgttctt
    13081 cgttaaagtc ggttcccgct tcccactgct tggcgagctg caaagcatac tcaaacaagc
    13141 cgtggaagag gcaaccgtca aagcgccact acgtcacaat gcggtagaaa tttttgacga
    13201 agtaaacacc ggcaaaaata ccggtagcgg cgtaccgtgg gtcacctggg acatcatccc
    13261 cgacaatgac gatgcggaaa tcgaagttta catggcaggc ggcggctgca cgctacctgg
    13321 ccgctcgaaa gtgttaatgc cgtcagaagg ctacgaaggc gtggtgaaat tcgtcttcga
    13381 aaatatctcc accctcgccg taaacgcctg tccaccggta ctggtgggcg tgggcatcgc
    13441 cacctcggtg gaaaccgccg ccgtactctc gcgtaaagcc attttgcgcc cgattggcag
    13501 ccgccatccc aatccaaaag cggcagaact ggagctacgc ctggaagaag gactcaaccg
    13561 tctggggatt ggtccacaag ggctgaccgg caacagttca gtgatgggcg catcgtcgcg
    13621 gcacgctgct ggttcatgcc gatctcacct ttgaaaatct gtctcacacc cggagcgcgt
    13681 tatgaaaaag atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcgcgtcgg
    13741 cgatgtgatc tacctgaccg gtacgctggt gacctgccgc gacgtttgtc accgccgttt
    13801 gatcgaactg aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg
    13861 ccccatcgtg cgcaaaaacg gcgacaaatg ggagatggtc tccgtcggcc cgacaaccag
    13921 tatgcgtatg gaaagttttg aacgtgaatt tattgagcag accggcgtaa aactggtggt
    13981 tggcaaaggt ggtatggggc cgctgaccga agaaggctgc cagaaattca aggcgttaca
    14041 tgtgattttc ccagcaggct gcgcggtgct ggcggcaacc caggtagaag agattgaaga
    14101 agtgcactgg acagaactcg ggatgccgga gtcactgtgg gtctgccggg tcaaagagtt
    14161 cggcccgctg attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa
    14221 gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat
    14281 caaataaccc tcccggagag gctcacccct ctcctttttc gcaggcataa cacgatgaaa
    14341 ccttccactg aatggtggcg atatcttgcg ccgctggcgg tcatcgccat tattgctcta
    14401 cttccggttc ccgcagggct ggagaaccat acctggctct actttgccgt ttttactggc
    14461 gtgatcgttg gactgatcct cgaacccgtg ccgggtgccg tggtggcgat ggtgggtatc
    14521 tccatcatcg ccatactctc tccctggctg ctgttcagcc ccgaacagct cgcccagcca
    14581 ggctttaagt tcaccgccaa atccctctcc tgggcagttt ctggtttttc taattcggtt
    14641 atctggctca ttttcgccgc ctttatgttt ggcacaggct atgaaaaaac cgggcttgga
    14701 cgccgcattg cgctgattct ggtgaaaaag atggggcatc gcacgctgtt tctcggctat
    14761 gcggtgatgt tctccgagct gatcctggca cctgtaacac cgtccaactc ggcgcgtggt
    14821 gcgggtatta tctatcccat catccgtaac ctgccaccgc tctatcaatc acaaccaaac
    14881 gacagcagtt cgcgcagcat tggctcgtac atcatgtgga tggggattgt tgccgactgc
    14941 gtgaccagcg ccattttcct gacggcgatg gcacctaact tgctgttaat tggactgatg
    15001 aaaagcgcat ctcacgccac gctgagttgg ggcgactggt tcctcgggat gttgccgctc
    15061 agcattttac tggttctgct ggttccctgg ctggcttacg tgctgtaccc gccggtactg
    15121 aagtctggtg atcaggtgcc gcgctgggca gagacggaac tgcaggcaat gggcccgctc
    15181 tgttcgcgtg aaaaacggat gctggggctg atggtaggcg cgctggtgct gtggattttc
    15241 ggcggtgatt atatcgatgc cgcgatggtc ggttacagcg tggtggcgct gatgctgctt
    15301 ctgcgcatta tcagttggga cgacattgtc agtaataaag cggcatggaa cgttttcttc
    15361 tggctggcct cgcttatcac cctcgcgacc ggactcaaca ataccggttt tattagctgg
    15421 tttggcaaac tgttagcagg cagcttaagt ggttattcgc cgacgatggt gatggtgacg
    15481 ttgattgtgg tgttttatct actgcgctac tttttcgcca gcgccacggc gtatacctgc
    15541 gccctcgcac cgatgatgat cgccgccgcg ctggcgatgc cggaaatccc gctgccggtg
    15601 ttctgcctga tggttggcgc ggcaattggt ctggggagca ttcttacacc atacgccacc
    15661 ggacccagtc cgatttacta cggtagtggt tatctgccaa cggcggatta ctggcgactg
    15721 ggggcgattt ttgggctgat attcctcgtg ttgctggtga ttaccggctt actgtggatg
    15781 cccgtggtgt tgctttaagt gaggacggtg ccggaaattg ctctccggca cccaatagtt
    15841 ttacgcagcc ggtaactccg ccagcggcca gcgcggacgc acgctaacgc cgagatccgc
    15901 cgtcgcgcct gctttaaacc gcaccattcc ggcataggcg atcatcgcgc cgttatcagt
    15961 acaaaattcc ggacgcgcgt agaacacttc gccgcggcgt tttttcatca tttcagccag
    16021 cttcgcccgt aacgtgcggt tagcactcac gccgcccgcc atgaccagtc gcttaaagcc
    16081 cgtctgatcc aacgctcgct tacacttaat catcaacgta tcgaccaccg catcttcaaa
    16141 ggcgcgggcg atatcagcac gcgtctggtc gtcggtgccg ttgtcacgaa tcgtgttcgc
    16201 cgcaaaggtt ttcagaccag aaaagctgaa atccagcccc ggacggtcgg tcatcggacg
    16261 cgggaaaaca aagcgcccgg cagtaccctg agccgccatt ttcgacagta acggtccgcc
    16321 aggataatcc agccccagca gcttcgcggt tttatcaaac gcttcaccgg cggcatcatc
    16381 gatagactcg ccgagcagct cgtactgacc aatgccagtc acgctgatta actgcgtatg
    16441 accgccggac accagcagcg cgacaaacgg aaattccggc gggttatctt ccagcatcgg
    16501 cgctaacaga tgcccttcca tatggtgtac cgggattgcc ggaacgttcc aggcaaacgc
    16561 cagagaacgc cccacggttg cgccaaccag cagtgcgccg actaatccag ggcctgcggt
    16621 ataggccaca gcatcaatgt cttttgccgt taaaccagac tcctttagcg ccgcctggat
    16681 caacggtacg gttttacgca catgatcgcg ggaggccagt tcaggcacga cgccgccgta
    16741 gtcagcgtgc aatttcacct gactatacaa ttggttggct aacaaacctt tttcatcgtc
    16801 gtaaatggcg atgccggttt catcgcagga agtttcaata cccagtacac gcatgacttg
    16861 ttttacctcg ctttattacc gcgcagtgta ggaccaatgc gggttgatgt aaaactttgt
    16921 tcgcccctgg agaaagcctc gtgtatactc ctcaccctta taaaagtccc tttcaaaaaa
    16981 ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata
    17041 agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta
    17101 attaaagtac gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc
    17161 gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc
    17221 gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa
    17281 aacgcacgcc gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga
    17341 gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt
    17401 gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg
    17461 cacgcactga catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga
    17521 atttccacgc gtgttgtcca ttccacaacg agaaaacccc gtcattcacc gttaacggtg
    17581 agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcctga
    17641 tgaactacga caagctcgag ttcgtcgaaa cggtcgaaga gctggcagca atgcacaatc
    17701 ttgaagtgcc atttgaagca ggcagcggcc ccagccagat cgagcgccat cagaggcaaa
    17761 cgctttatca gttgatggac ggtctgaata cgttttacca acaatcttta caacaacctg
    17821 ttgccacgtc tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgctc
    17881 gctttgcgat tggttttgcg ccccccggct gggacaacgt cctgaagcgg tttggcggca
    17941 atccagaaaa tcgccagtca ttgattgatg cggggatgtt ggtcactaac gatcagggac
    18001 gcagttacga tcgtttccgc gaacgggtga tgttccccat tcgcgataaa cgcggtcggg
    18061 tgattggttt tggcgggcgc gtgctgggca acgatacccc caaatacctg aactcgccgg
    18121 aaacagacat tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata
    18181 acgctgaacc caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc
    18241 aatacggcat taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac
    18301 aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc
    18361 gcgatgccgc atggcgagcg ctggaaacgg cgctgcctta catgacagac ggccgtcagc
    18421 tacgctttat gtttttgccg gatggcgaag accctgacac gctggtacga aaagaaggta
    18481 aagaagcgtt tgaagcgcgg atggagcagg cgatgccact ctccgcattt ctgtttaaca
    18541 gtctgatgcc gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg
    18601 cactgccatt gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat
    18661 taggcaacaa attaggcata cttgatgaca gccagcttga acgattaatg ccaaaagcgg
    18721 cagagagcgg cgtttctcgc cctgttccgc agctaaaacg cacgaccatg cgtatactta
    18781 tagggttgct ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg
    18841 atgaaaataa gctccctgga cttggcttat tcagagaact ggtcaacact tgtctctccc
    18901 agccaggtct gaccaccggg caacttttag agcactatcg tggtacaaat aatgctgcca
    18961 cccttgaaaa actgtcgatg tgggacgata tagcagataa gaatattgct gagcaaacct
    19021 tcaccgactc actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa
    19081 tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa
    19141 accaggagct ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc
    19201 cccgacagcc gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt
    19261 ggcggtaaac aaccgttgga tttcagcgtt aacggctgaa ggacatcggg tcaatcgccc
    19321 aacaccaacc tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca
    19381 gctgaaactt cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa
    19441 tgaccatctg ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat
    19501 caacgacatg ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc
    19561 tgaaaacacc gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga
    19621 atctgaaatc gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt
    19681 tgaactgttg acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa
    19741 ccaggttcaa tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta
    19801 cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc
    19861 gaacgcagaa gaagatctgg cacctaccgc cactcacgtc ggttctgagc tttcccagga
    19921 agatctggac gatgacgaag atgaagacga agaagatggc gatgacgaca gcgccgatga
    19981 tgacaacagc atcgacccgg aactggctcg cgaaaaattt gcggaactac gcgctcagta
    20041 cgttgtaacg cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg ctcaggaaga
    20101 gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta
    20161 cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat
    20221 gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg
    20281 caacgaaacc agcgatacct ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga
    20341 aaaactgcac gatgtctctg aagaagtgca ccgcgccctg caaaaactgc agcagattga
    20401 agaagaaacc ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg
    20461 tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac gtctggttat
    20521 ttctatcgcc aagaaataca ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg
    20581 taacatcggc ctgatgaaag cggttgataa attcgaatac cgccgtggtt acaagttctc
    20641 cacctacgca acctggtgga tccgtcaggc gatcacccgt tctatcgcgg atcaggcgcg
    20701 caccatccgt attccggtgc atatgattga gactatcaac aaactcaacc gtatttctcg
    20761 ccagatgctg caagagatgg gccgtgaacc gacgccggaa gaactggctg aacgtatgct
    20821 gatgccggaa gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa tctccatgga
    20881 aacgccgatc ggtgatgatg aagattcgca tctgggggat ttcatcgagg ataccaccct
    20941 cgagctgccg ctggattctg cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct
    21001 ggctggcctg accgcgcgtg aagcaaaagt tctgcgtatg cgtttcggta tcgatatgaa
    21061 caccgaccac acgctggaag aagtgggtaa acagttcgac gttacccgcg aacgtatccg
    21121 tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg aagtgttgcg
    21181 tagcttcctg gacgattaat cggtaggccg gatcaggcgt tacgccgcac ccggcactag
    21241 tctctctgca caaacgccac cttt
//
LOCUS       DADLTY010000066        21253 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.066, whole genome shotgun sequence.
ACCESSION   DADLTY010000066 DADLTY010000000
VERSION     DADLTY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.066"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            18..791
                     /locus_tag="JN334_002025"
                     /pseudo
     CDS             18..791
                     /locus_tag="JN334_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(851..2149)
                     /gene="eno"
                     /locus_tag="JN334_002026"
     CDS             complement(851..2149)
                     /gene="eno"
                     /locus_tag="JN334_002026"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAY7438169.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="JN334_002027"
     CDS             complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="JN334_002027"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HAY7438170.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="JN334_002028"
     CDS             complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="JN334_002028"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HAY7438171.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4989..7223)
                     /locus_tag="JN334_002029"
     CDS             complement(4989..7223)
                     /locus_tag="JN334_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAY7438172.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="JN334_002030"
     CDS             complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="JN334_002030"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAY7438173.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8629..11385
                     /gene="barA"
                     /locus_tag="JN334_002031"
     CDS             8629..11385
                     /gene="barA"
                     /locus_tag="JN334_002031"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HAY7438174.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="JN334_002032"
     CDS             complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="JN334_002032"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY7438175.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMEQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12978..14318)
                     /locus_tag="JN334_002033"
     CDS             complement(12978..14318)
                     /locus_tag="JN334_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY7438176.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14320..15672)
                     /locus_tag="JN334_002034"
     CDS             complement(14320..15672)
                     /locus_tag="JN334_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7438177.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16106..16555)
                     /locus_tag="JN334_002035"
     CDS             complement(16106..16555)
                     /locus_tag="JN334_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAY7438178.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16573..17355)
                     /gene="truC"
                     /locus_tag="JN334_002036"
     CDS             complement(16573..17355)
                     /gene="truC"
                     /locus_tag="JN334_002036"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HAY7438179.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17355..17684)
                     /locus_tag="JN334_002037"
     CDS             complement(17355..17684)
                     /locus_tag="JN334_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HAY7438180.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18306..18851)
                     /gene="syd"
                     /locus_tag="JN334_002038"
     CDS             complement(18306..18851)
                     /gene="syd"
                     /locus_tag="JN334_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HAY7438181.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18919..19767
                     /gene="queF"
                     /locus_tag="JN334_002039"
     CDS             18919..19767
                     /gene="queF"
                     /locus_tag="JN334_002039"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAY7438182.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19879..21243
                     /gene="ppnN"
                     /locus_tag="JN334_002040"
     CDS             19879..21243
                     /gene="ppnN"
                     /locus_tag="JN334_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HAY7438183.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5230 a         5671 c         5065 g         5287 t
ORIGIN      
        1 ttgcagccca accaactatt gtcccacaat tacagcaaca agttacgggt cttacgagta
       61 gcttaaactc acaagaaaag aaagaactga cgcacaagtt agaatctatt ttcaataata
      121 cccaagtgca aattgctgta ttaattgttt ccacaaccaa agatgagaca attgaacaat
      181 atgctacaag agtttttgac aattggcgtt taggagatgc caaacgtaat gatgggatac
      241 tgatcgttgt tgcctagtcg gatcgcactg tccgcatcca ggtaggttat gggctggaag
      301 aaaaggtaac cgatgctctg gctggagata tcatccgtag caacatgata cccgcattta
      361 aacaacaaaa attagctaag ggattagagt tagctataat cgctttgaat aaccaactca
      421 cttcacaaca ccaatatccg gctaatcctt cagaaagtga atcaacgtct tccactgatc
      481 attattactt tgctattttt tgggtatttg cagtgatgtt cttccctttc tggttttttc
      541 atcaaggcag taatttttgt cgcgcatgta aaagtggagt ctgtatttca gcaatccatc
      601 ttttagattt attcctgttc tccgataaaa ctttttccat tgctgtattt tccttctttt
      661 ttactttcac catatttatg gtctttacct gtttatgtgt acttcagaaa agagcatctg
      721 gtagaagtta tcattcagac aacagcggtt ccgctggagg ctcaagtggc ggcgcatccg
      781 gccgctggta actccaccat aaaaaatgcc agcccgaagg ctggcattat caaatcagat
      841 aaagtcagac ttatgcctgg cctttgatct ctttacgacc gttgtacggt gctttttcgc
      901 ccagagcttc ttcgatacga atcagctggt tgtatttagc aacacggtca gaacggctca
      961 tagaaccagt tttgatctgg cctgcagcag taccaacagc cagatcagcg atggtagcat
     1021 cttcagtttc accagaacgg tgagagataa ctgcagtgta gccagcatct ttcgccatct
     1081 tgattgcagc cagagtttcg gtcagagaac cgatctggtt gaatttgatc aggatggagt
     1141 tagcgatacc tttttcgata ccttctttca ggatcttggt gttggttacg aacaggtcgt
     1201 caccaaccag ctggattttg tcgcccagaa ctttggtctg gtatgcgaaa ccgtcccagt
     1261 cagattcgtc cagaccgtct tcgatagaaa cgatcgggta ctgtttggtc agttcttcca
     1321 ggaagtgagt gaattcttca gaggtgaacg ctttgttgcc ttcgccagcc agaacgtatt
     1381 taccatcttt gtagaattca gaagctgcgc agtccatcgc cagagtgatg tctttgccca
     1441 gttcataacc agcagcttta acagcttcag cgataacagc cagagcttca gcgttggaac
     1501 ccaggttcgg cgcatagcca ccttcgtcac caacagcagt gttcatgcct ttcgctttca
     1561 gaacttttgc caggtgatgg aaaacttcag aacccatgcg gatggcttct ttcacagttt
     1621 tcgcgccaac cggctgaatc atgaattcct ggatatcaac gttgttgtca gcgtgctcac
     1681 caccgttgat gatgttcatc atcggaaccg gcatggagta tttgcccgga gtgccgttca
     1741 gttcagcgat gtgctcgtac agcggcatac ctttagcagc tgcagcagct ttggcgttag
     1801 ccagagatac agccaggatt gcgttcgcgc cgaatttgga tttgttttcg gtgccgtcca
     1861 ggtcgatcat gatcttgtca atgccagcct gatctttagc atctttgcca atcagcgcct
     1921 gagcgatcgg gccgtttacc gcagcaacag ctttggttac gcctttaccc aggaaacggg
     1981 atttgtcgcc atcgcgcagt tccagagctt cacgggaacc agtagaagca cctgacggag
     2041 cagctgccat accgacgaaa ccaccctcca gatgtacttc ggcttcaaca gtcgggttac
     2101 cacgggagtc gatgatttca cgaccgatga tttttacgat tttggacatt aggttttcct
     2161 caagtcacta gttaaactga aactccagac aaacaacgcg tacccaaggt acgcgttgcc
     2221 gttctaactt ttttacttac ttcgcctgac gtttctggaa ctcgctggcg gctttcacaa
     2281 agcctgcaaa cagcgggtga ccatcacgtg gagtagaagt aaactccgga tggaactggc
     2341 aagccacgaa ccacgggtga ttcggaactt cgatgatctc gaccaactga tcatccccgg
     2401 aacggcccgc aacgcgcaga cctgcatctt caatctgttt caacagcatg ttgttgactt
     2461 cgtaacggtg acgatgacgc tcaacaattg tcggcgcatt gtatagctgg cgaaccaggc
     2521 tatcgtcaac caactggcac tgctgtgcgc cgagacgcat ggtaccgccg agatcgctct
     2581 tctcgctacg aacttcaacg ttgccgtttt catcgcgcca ctcggtaatc agcgccacaa
     2641 ccgggtactt acagtctggc acaaattctg tagagttggc gttctccatg ttggcaacat
     2701 ggcgagcgta atcaattaac gccacctgca tacccaggca aatgcccaga taaggaatat
     2761 tgttctcacg cgcaaaacgc gcggtcgtaa tcatgccttc tacgccacga tagccgaaac
     2821 cgccaggtac gaggattgcg tccagacctt taaggatttc aacgccgcgc gtttcaacat
     2881 cttgtgaatc gatcagtttg atgttgacgc tgacacgatt cttcagccca ccgtgtttca
     2941 gtgcttcgat cactgattta taagcatccg gcagttcaat gtacttgccg accataccga
     3001 tggtgacttc acttaccgga ttcgcttctt cgaagataac ctgttcccat tcggacagat
     3061 ttgcttccgg gcagtttaag ctgaatcgtt tacaaatata atcgtccagc ccctgagatt
     3121 tcaacaggcc cggaatttta tagatggaat cgacgtcttt cagagaaata accgcttttt
     3181 ccggaacatt acagaacaat gcaatcttcg cacgttcgtt cgccggtaca gcgcgatctg
     3241 aacgacaaat caggatgtca ggctggatac cgatggagag cagctctttt acagagtgct
     3301 gagtcggttt ggttttgact tcaccagacg ctgccatgta cggcaccagc gtcaggtgca
     3361 taaacagagt gtgctcacgg ccaatttcaa cagccatctg gcgaatagct tcgaggaacg
     3421 gcaaggattc gatatcacct actgtaccgc cgatttctac cagtactacg tcatgacctt
     3481 cgccaccttc cagcacgcgc tctttgattg cgttagtgat gtgcggaata acctgcacgg
     3541 ttgcgccgag gtagtcaccg cggcgttctt tacgcagaac gtcagagtag atacgacccg
     3601 tggtgaagtt gttgcggcgg ctcattttgg tacgaatgaa acgctcgtag tgccccaggt
     3661 ccaagtcggt ttcagcgccg tcttcagtaa cgaacacttc cccgtgttgg attgggctca
     3721 tagtacctgg atcgacgttg atgtaaggat ccagtttcat gatggtcaca ttgaggccac
     3781 gggcttcaag aatggctgcg agggaggctg cggcaatgcc tttacccaga gaggatacga
     3841 ccccgccggt cacaaaaata tagttcgttg tcatgctgaa cctgagaagt taggttgaaa
     3901 agacgatgga ataaccaaga cgggaaagca gtatacccga acatgacctg tgccacaaac
     3961 tttcattatc cctcctcttc gccagcgcac tattgaaatc aggagtgaga aaatagcccc
     4021 tttggggtaa atgtttttga cgcaaatcaa gcgcttgtca tttaaaaaat gacacaaatg
     4081 gcgcttgacc gcgtaattcc cttagagatc aatttcctgc cgttttacct gttgccagac
     4141 ttcttccatt gtttcgaggt caacacctgt catttccagt ccacgcgcgg caacaatacg
     4201 ctccacttcg cgaaaacgac gctcgaattt ttcgttcact ttttgcaatg cgatttctgc
     4261 tttcgtccct aaatggcgag ccagattaac cgtggcaaac agcaggtccc ccatttcctc
     4321 ctccagttta gcctggtcga caacagcctg ccgcgcctcg tacatcacct cgtcgatctc
     4381 ttcgtagact ttatcaacca ccgggccaag cgtcgtccag tcgaagccaa cgttggcgca
     4441 acgtttctgg attttttgcg cccgcattaa agccggtaaa ctacgaggaa tatcgtccag
     4501 cgccgaatgc tgcgctttct gcgcgcgctc ttcggttttg atttgctccc aacgggcaag
     4561 cacttcacta ctgttttcgg cagaactatc agcaaaaaca tgcggatggc gacgctctaa
     4621 tttatcgcta atagcagcgc aaatatcatt aaagtcaaag cgcccttctt cctgagccat
     4681 ttgcgcgtaa aacaccacct ggaatagcag atcgcccagt tcaccgcgaa gatcgtcaaa
     4741 atcttcacgg gcgatggcgt ccagcacttc gtaggtttct tcaagggtgt aaggcgcaat
     4801 ggtggcaaat gtctgctctt tatcccacgg gcagccgttt tccggatcgc gcaggcgctg
     4861 cataatagtg agcaaacggt cgatttgatt cattgaattg tcctgaaaat tgcgggtctg
     4921 tcaggtgtaa taccttcgag taaaagatat cgctggatac ggctgacgcc atatccagct
     4981 atgtaagact aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     5041 ttacccagca cgcgccccag cacttgcagg ttgtaaatct caatggtcat gtcgatggtc
     5101 gccagttgct gtttggtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     5161 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5221 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5281 gcatgggagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgt
     5341 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5401 tggtgcatca ggttaccaac accttcaacc accacacgac cgttatcttt actgcggttt
     5461 tgcggtgtgt agcttttttg ctgaagctgt ttcagcgcgg cggcgtcctg ctcttcggca
     5521 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5581 ccaccaatcg ccgccagcaa ctcgtcgaca tcattgaagt tgtaacgcgg cagcagatgt
     5641 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5701 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aggcgtgaat tttcgaacgc
     5761 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5821 tgtttctggg tgataatttc aatctggtcg cccatttgca gctggtaggt gaacggcaca
     5881 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5941 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     6001 acatacaccc ggtcgtcaaa cacctgacta cgtacttcgt cgagcatttc gccggaatca
     6061 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     6121 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6181 tccgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt tccacccggc
     6241 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacgtaatcg
     6301 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcataacaa
     6361 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcgaaggcg
     6421 aggttctttt tctgcatttt gcgccagatg ctgtagatgt gtttcggacg accatacact
     6481 tcagctttaa cgccttcagc tttcatctca gcgcgcagat ggccgacaaa ctcttcgata
     6541 tagtgttcgc ggtcgagacg gcgttcatgc agcagttttg cgatgcgttt gtattcggtc
     6601 gggtggagat aacggaagca gtaatcttcc agctcccatt tcagttgccc aatcccaaga
     6661 cggttcgcca acggcgcgta gatattggtg cactcttttg ccgccagtac acgttcatct
     6721 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6781 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6841 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6901 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6961 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     7021 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     7081 ccctgcgtct gttgcagaca atacgcccag gtttcggcta agcactcaca cgacttctgg
     7141 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7201 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7261 caaatcgtaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7321 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7381 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7441 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7501 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7561 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgcag
     7621 gaacaccttc tacaccgacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7681 ccataccgca gaacagatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7741 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7801 aagttaagcg caacccgttt gagtcatacc agggcatctc accagagacg gtttcgagta
     7861 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7921 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7981 ttgcctgtac cagttcaaca tgaccaagat ggcgcatagc ttgtaagctg cccagacatg
     8041 ccctgacttt gggcagcaat gcttcaagtt ggggcgctaa aatggggcat tgtttgacgt
     8101 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     8161 agtagttcag acttaaacgc gcgcggcggc gatagcccca gggaacatcg gcgatcactt
     8221 cagagacatc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8281 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8341 tttcgcgttc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8401 tatcttcagt aacagcaact tccgcgtttt cctgcggcaa taatccgggg ataaataacg
     8461 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8521 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8581 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8641 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8701 ctgagtatct ttttcgtcgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8761 ggtgctagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8821 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8881 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8941 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     9001 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     9061 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gcttgagctg
     9121 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttcctg cgtgatgatg
     9181 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9241 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9301 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9361 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9421 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttggatct ggcgaaaaag
     9481 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9541 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9601 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9661 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9721 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9781 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9841 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9901 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9961 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
    10021 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccgggctg
    10081 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
    10141 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10201 attatcgaag ggccatccat ccagtgcctc gcaggtaagc gcctggccta tgtcgaacca
    10261 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10321 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10381 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10441 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagttaatgc tgaaaaactc
    10501 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10561 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagt
    10621 aagctggcaa tgacagtcat ggcggttgat gacaaccccg ctaacctgaa acttatcggc
    10681 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcagtt
    10741 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gccggacatg
    10801 gatggcattc gggcctgcga actcatccac caactcccgc atcagcgaca aacgccggtt
    10861 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10921 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10981 aagcctggca gcggtatttc gtctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    11041 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    11101 ttagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    11161 gaggaacagc tggctggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11221 ggcagttgcg gctatagcgg cgtaccacgt atgaagaatc tctgccagct gatcgaacaa
    11281 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11341 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11401 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11461 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11521 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11581 accggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11641 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11701 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgctccatat cgatttctac
    11761 acccagcccc ggtttttctg gcacctgtac cagcccgcct ttgatctcaa acggttcttt
    11821 ggtcaggcgc tgattacctt cctgccagat ccagtgcgta tcaatagcag taattttacc
    11881 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11941 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    12001 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    12061 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    12121 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccac acggatcttc
    12181 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12241 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12301 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12361 atatttttca tacgccgctt ccgccaggcg caccaccgca tccggcgtca tcgcttcttc
    12421 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12481 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12541 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12601 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12661 gcgcccacta gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12721 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12781 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12841 attacgcgtg aagaacggtg cgtgtgcacc actcagattc atcagcatac tgtcatgacc
    12901 cgccaccggg atgacctgca tttcagtaac aacaggcgtc gtaaattgag aactcatatt
    12961 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    13021 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgcaagg cagacgttta
    13081 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    13141 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13201 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13261 acatgggtaa atatcgccag agagatatcg aaatggttgt tggaatggca gccccaggtc
    13321 agcccccagt cgtcgcaaag ctgcgccacg cggactgcac cggaaagcgt ccagaagtgc
    13381 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13441 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgtcgaaa ctccgccatc
    13501 acttcacgtc cggagaagcc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13561 tcattcagcc ctttgcataa agaaatggct tcatcaagca gccatgcacc gttgggatca
    13621 acggtaatcc gcgcatccgg gaagcttttc ttcaatgcac gaacagtgtc gatttcttgc
    13681 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13741 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13801 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcacct
    13861 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13921 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13981 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccaaaggt atcaaaatcg
    14041 gctgcctgat tacctttatg tacctgctga accactttat tcaggcgcgc aacttcctgg
    14101 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    14161 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca cgatattgcg ggtgaagtat
    14221 gcgttatgcg caccaccaat attgagcaac atgctgtcat gtccggcgac cggaatgact
    14281 ttcatatcag taataacagg actggattgt gtcgccatct tattgccccg cgacaggttt
    14341 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattagggc
    14401 atgaacacca acataaatca gcgccccatt aaacgagcca gtcgtgccaa cgatataacc
    14461 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14521 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14581 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14641 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14701 aatgttcagc gatcccgtgc ggcgcatcag ccagtcggaa ataattccac ccagcacacc
    14761 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14821 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14881 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gctgtttgat
    14941 ctgcccccac ttcacgctga acggcacttt aactttggtg ctttgctgat ccatattgat
    15001 cagcgcgcca cccgcggcga tatactccag ctctttctga tttacccccg gatgttgatt
    15061 tggctcgtgg atgactttca accagataaa gctgatgaca atgcccagac cgcccataaa
    15121 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15181 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcac
    15241 cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc
    15301 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15361 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15421 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15481 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15541 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15601 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15661 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15721 acatcgccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15781 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15841 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15901 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gtaaaaacga
    15961 agaaataaag aagagtatga aatggatcgc ttgactctag gcaaacgcca gtaaaaatcc
    16021 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    16081 acgacattta ccgctcgctg acatatcagg acaacagcgt gccccactgt tcgacccacg
    16141 gatttgattc cgtttccggt tccgggttct cactggcgtc aatcaacagc atttcgccaa
    16201 cccgctgagc gctctgttcc tgcaacaagg catcgaactg tttgccgcca ttgcaaaaat
    16261 ttacataact gctgtcgccg agcgcaatca cgccataacg cagattcggc tggaagccca
    16321 gactatcttt gattccctga aagagcggca caatgctatc aggaaggtcg ccctgcccgg
    16381 tcgtggacgt aaccaccaga acatacttat cctgataggg cagccagtcg cttaattcag
    16441 gatcttcaaa taccgttgct ttgtggccct gtgcggtcag aattgcttct gcttcttccg
    16501 ccactaacag cgaatttccg tacatggtgc cgacaaaaat accaatttcc gccataccgt
    16561 tttctccctg gattaggaac ttatctctcc atcctgaccc gatggcgcac tgaactcaac
    16621 cctttcattt tcaggaagca gaccgcgcca gccaaattgt gataacgcct gcatccaggt
    16681 gtcgtccagg cccgcgtgaa tagtcagcgg ctcgccagta aaaggatgcg tcaatgacaa
    16741 ctgactggca tgtagcatta accgctggag gccaaaatgc tcagcaccgc tgcgattctg
    16801 gcgtaaatcg ccatgtttgc tatcgccaat aatcggatga cgcaaatggg caagatgtcg
    16861 tcgaagctga tgtttgcgtc cggttttcgg ctccagttcc accaggccgt agcgcgtggt
    16921 cgggtaacgt ccggtcgcta ccggcatttc tacggtcgcc agaccgcgat aatgcgtcac
    16981 tgctggctgc gggcctttat cttcgcgggc aaatttatca gcgattttgt ccagttcttc
    17041 caccagtgga taatccaaca ccgcttcttc catcaaccag ccgcgcacaa tcgcatggta
    17101 acgtttctgg atttggtgct gttcaaactg ttgtgccagc agccgtccgg cctcgctgga
    17161 taatcccatc aacaacacac cagaagtggg tcggtccaga cgatgagcag taaaaacatg
    17221 ctgtcctatc tggtcacgca cggtttgcat gaccactact ttctcgtcgc gatccagcca
    17281 gctacggtga accagccagc cggagggttt atttaccgca accagccatt catcctgata
    17341 gagtatttcc agcattagct cgcatcatcc gcaaaaagag catccagttt ttccaactca
    17401 gccagaataa gcgcgcgttg cggatggtcc gtcgccagcg ccatttcata atagggtgca
    17461 acagcaaaag cgcccggtaa cggctggttg ttatttaaca aatcgtgcat tcgcgggatc
    17521 agcccccact gcaaccactc cagtggttcc atggtgtcca taaagaacgg ttgggtacta
    17581 ttaaattgat gcggctgggg ttcatcgttt cgccagtgct gatgttcacg cagtaacgct
    17641 tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg tcataaagca acctcaataa
    17701 gaaaaactgc cgcgaaggat agcaggaata aaaaaaggga gcactgtatt cacagtgctc
    17761 ccggttcgtt tcgcagcatt ccagctactt ttgttgctcc ctgctcatcc ttgacaactt
    17821 ttcctctggc cttgcggcca atcgttcatc ctgaactatt gcttcctgct cacaccaccc
    17881 cgatgtgata cttcatcctg aagtgtccct ggccttcctg acccaccgaa tcatcctgac
    17941 cggttctcat tctccatcct ggaggtgtcc tttaacgcgt cctgcgtcat cctcttcgct
    18001 tcatccagaa gcctttccct gaaacaccat cctggtgtgt cctgcagaag tgtcatcatc
    18061 ctgatgttca cttcgttgtc tgactccctg tcgacgaaga tagaatcgtc tttttcgcga
    18121 agtcttacaa ggcgcttaca gacaatgcct taaggaaaat ttcatatgaa agttaagaat
    18181 taacaatcaa cattttgatt ttaaatgaaa ataacgattc ttatcttttt aagtattcgc
    18241 ttatttccta gcgatctcct acagagtctg taagagatct ctcacaacca gatggggtat
    18301 ttggattaca gaagaggctt aagttgatta aggaattccg caagatttga ggctaaattc
    18361 gtccgtttac gtgttcccag cgtctctttg cacacttcac cggagagatt acacaccgag
    18421 atgacctcta actcttcttc cagcgtcgcg ataaatagcg tgggcggcag ttttagcctt
    18481 ttctgagtca ccagatgacc aatcagattt tcctgcaccc gacggaagtc gtcttcactc
    18541 caggtttgca gcaatgtcag tttgatatcg ccaaactgcg cgtgcatatc cccggcaaac
    18601 tgagtcgtat aaaaggtatg aattgtgggt tgtatcacaa tatcaaaagc gcgttcaacc
    18661 gcgtttacat tttgttcccc ggtaaacggc tggggttgcc agtatacggc atcttcggta
    18721 gttgaaataa tgcacggtga aggaacaccg tacaattcct cacttaacgg ccagctttta
    18781 tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat ctttcagggc ttgtgcggtc
    18841 aaatcgtcca ctaatttctc tcttcactta aaccagatac acttgtcgtt tagtttatct
    18901 ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc aggcacttgc gggcctgact
    18961 cttggaaaat caaccgatta ccgggatacc tatgacgcca gcctactgca aggcgttcca
    19021 cgcagcctga atcgcgaccc gctgggtctg aaagcggata acctgccttt tcacggtacg
    19081 gatatctgga cgctgtatga actttcctgg ctgaatgcga aaggtttgcc gcaggtcgct
    19141 gtcggtcatg ttgaacttga ttacaccagc gtaaatctga ttgagtcgaa gagttttaag
    19201 ctctatctca acagttttaa ccagacgcgt tttaataact gggatgaggt gcgccagacg
    19261 ctggagcgcg acttaagcac ttgcgctcag ggtaagatta gcgtggcgtt atatcgtctt
    19321 gatgaactgg aaggccagcc gataggtcat ttcaatggca cttgcattga tgaccaggat
    19381 atcactatcg ataactatga attcactact gactatctgg agaatgccac cagcggtgaa
    19441 aaagtagtgg aagagacgct cgtcagccac ctgctgaaat caaactgcct gatcacccat
    19501 caaccagatt ggggttcgat ccaaattcag tatcgtggac gccaaattga cagagaaaaa
    19561 ctgctgcgtt acctggtctc attccgtcat cacaacgagt tccacgaaca gtgcgtggaa
    19621 cgcatcttta atgacctgtt acgcttctgc cagccagaga aattgagcgt ttacgcacgt
    19681 tatacccgtc gtggcggtct ggacattaac ccgtggcgca gtaatagcga ttttgtccca
    19741 tcgaccacaa gactggttcg gcaataaatt ttttctcaat tttgcgtgct ggattcacgc
    19801 agaaggttgt gaaaggtcat caggcagggc tattgtaatc aaagggaatg acgatattcg
    19861 tcccataagg agtttttctt gattacacat attagcccgc ttggctccat ggatatgttg
    19921 tcgcagctgg aagtggatat gcttaaacgc accgccagca gcgacctcta tcaactgttt
    19981 cgcaactgtt cacttgccgt actgaactcc ggtagtttga ccgataacag caaagaattg
    20041 ctgtctcgtt ttgaaaattt cgatattaac gtcctgcgcc gtgaacgcgg cgtaaagctg
    20101 gaactgatta atcccccgga agaggctttt gtcgatgggc gaattattcg cgctttgcag
    20161 gccaacttgt tcgcggttct gcgagacatt ctcttcgttt acgggcaaat ccataatacc
    20221 gttcgttttc ccaacctgaa tctcgacaac tccgtccaca tcactaacct ggtcttttcc
    20281 atcttacgta acgctcgcgc gctgcatgtg ggtgaagcgc caaatatggt ggtctgctgg
    20341 ggcggtcact caattaacga aaacgagtat ttgtatgccc gtcgcgtcgg gaaccagctg
    20401 ggcctgcgtg agctgaatat ctgcaccggc tgtggtccag gagcgatgga agcgccgatg
    20461 aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag acagtcgttt tattggtatg
    20521 acagagccgt cgattatcgc cgctgaaccg cctaacccac tggtcaacga attgatcatc
    20581 atgccggata tcgaaaaacg tctggaagcg tttgtccgta tcgctcacgg catcattatc
    20641 ttccctggcg gtgtgggtac ggcagaagag ttgctgtatt tgctgggaat tttaatgaac
    20701 ccggccaaca aagatcaggt tttaccattg atcctcaccg gcccgaaaga gagcgccgac
    20761 tacttccgcg tactggacga gtttgtcgta catacgctgg gcgaaaacgc gcgccgccat
    20821 taccgcatca tcattgatga cgccgctgaa gtcgctcgtc agatgaaaaa atcgatgccg
    20881 ctggtgaaag aaaatcgccg tgatacaggc gatgcctaca gctttaactg gtcaatgcgc
    20941 attgcgccag atttgcaaat gccgtttgag ccgtctcacg agaatatggc taatctgaag
    21001 ctttacccgg atcaacctgt tgaagtgctg gctgccgacc tgcgccgtgc gttctccggt
    21061 attgtggcgg gtaacgtaaa agaagtcggt attcgcgcca ttgaagagtt tggtccttac
    21121 aaaatcaacg gcgataaaga gattatgcgt cgtatggacg acctgctaca gggttttgtt
    21181 gcccagcatc gtatgaagtt gccaggctca gcctacatcc cttgctacga aatctgcacg
    21241 taatctcccc tcc
//
LOCUS       DADLTY010000067        21084 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.067, whole genome shotgun sequence.
ACCESSION   DADLTY010000067 DADLTY010000000
VERSION     DADLTY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.067"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(1..386)
                     /locus_tag="JN334_002041"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="JN334_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="JN334_002042"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="JN334_002042"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAY7438184.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="JN334_002043"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="JN334_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAY7438185.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="JN334_002044"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="JN334_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAY7438186.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="JN334_002045"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="JN334_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAY7438187.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     gene            4210..4254
                     /locus_tag="JN334_002046"
     CDS             4210..4254
                     /locus_tag="JN334_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HAY7438188.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="JN334_002047"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="JN334_002047"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAY7438189.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="JN334_002048"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="JN334_002048"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAY7438190.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="JN334_002049"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="JN334_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAY7438191.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="JN334_002050"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="JN334_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HAY7438192.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="JN334_002051"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="JN334_002051"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HAY7438193.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="JN334_002052"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="JN334_002052"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HAY7438194.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="JN334_002053"
     CDS             10748..11212
                     /locus_tag="JN334_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7438195.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="JN334_002054"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="JN334_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HAY7438196.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /locus_tag="JN334_002055"
     CDS             12236..13672
                     /locus_tag="JN334_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HAY7438197.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="JN334_002056"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="JN334_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HAY7438198.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="JN334_002057"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="JN334_002057"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HAY7438199.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="JN334_002058"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="JN334_002058"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HAY7438200.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="JN334_002059"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="JN334_002059"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAY7438201.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20502)
                     /gene="fadK"
                     /locus_tag="JN334_002060"
     CDS             complement(18802..20502)
                     /gene="fadK"
                     /locus_tag="JN334_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HAY7438202.1"
                     /translation="MHPTGPQLGPDVLFRESKMKVTLTFNEQRRAAYRQQGLWGDASL
                     ADYWQQTARAMPDKIAVVDNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPG
                     WCEFTVIYLACLKIGAVSVPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLIL
                     PLQNQLPQLQQIVGVDKLAPATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTE
                     GLPKGVMLTHNNILASERAYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARS
                     VLLDIFTPDACLALLEQQRCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIP
                     KKVARECQQRSIKLLSVYGSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDAR
                     KTLPPGCEGEEASRGPNVFMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGR
                     KKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLS
                     LEEMAAFFSRKRVAKYKYPEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE
                     "
     gene            complement(20505..20798)
                     /locus_tag="JN334_002061"
     CDS             complement(20505..20798)
                     /locus_tag="JN334_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY7438203.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..>21084)
                     /locus_tag="JN334_002062"
     CDS             complement(20795..>21084)
                     /locus_tag="JN334_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438204.1"
                     /translation="KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFS
                     QYPRMVADIMNEMFTIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
BASE COUNT         5258 a         5008 c         5578 g         5240 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttgg
//
LOCUS       DADLTY010000068        21038 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.068, whole genome shotgun sequence.
ACCESSION   DADLTY010000068 DADLTY010000000
VERSION     DADLTY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.068"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..367
                     /locus_tag="JN334_002063"
     CDS             <1..367
                     /locus_tag="JN334_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAY7438205.1"
                     /translation="REQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIE
                     QPQEGYAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLI
                     ALAPGESTTSEMSLRVEWL"
     gene            complement(415..837)
                     /locus_tag="JN334_002064"
     CDS             complement(415..837)
                     /locus_tag="JN334_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY7438206.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(934..2151)
                     /locus_tag="JN334_002065"
     CDS             complement(934..2151)
                     /locus_tag="JN334_002065"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HAY7438207.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            complement(2161..2973)
                     /locus_tag="JN334_002066"
     CDS             complement(2161..2973)
                     /locus_tag="JN334_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HAY7438208.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(2970..3290)
                     /locus_tag="JN334_002067"
     CDS             complement(2970..3290)
                     /locus_tag="JN334_002067"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HAY7438209.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDFP
                     EAQP"
     gene            complement(3290..3808)
                     /locus_tag="JN334_002068"
     CDS             complement(3290..3808)
                     /locus_tag="JN334_002068"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HAY7438210.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(3805..5166)
                     /locus_tag="JN334_002069"
     CDS             complement(3805..5166)
                     /locus_tag="JN334_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HAY7438211.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            5302..6192
                     /gene="hcaR"
                     /locus_tag="JN334_002070"
     CDS             5302..6192
                     /gene="hcaR"
                     /locus_tag="JN334_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HAY7438212.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            6352..7491
                     /locus_tag="JN334_002071"
     CDS             6352..7491
                     /locus_tag="JN334_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HAY7438213.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(7483..8763)
                     /gene="csiE"
                     /locus_tag="JN334_002072"
     CDS             complement(7483..8763)
                     /gene="csiE"
                     /locus_tag="JN334_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HAY7438214.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(8954..9808)
                     /locus_tag="JN334_002073"
     CDS             complement(8954..9808)
                     /locus_tag="JN334_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7438215.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            complement(9953..10756)
                     /gene="suhB"
                     /locus_tag="JN334_002074"
     CDS             complement(9953..10756)
                     /gene="suhB"
                     /locus_tag="JN334_002074"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HAY7438216.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            10875..11615
                     /gene="trmJ"
                     /locus_tag="JN334_002075"
     CDS             10875..11615
                     /gene="trmJ"
                     /locus_tag="JN334_002075"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAY7438217.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            11885..12373
                     /gene="iscR"
                     /locus_tag="JN334_002076"
     CDS             11885..12373
                     /gene="iscR"
                     /locus_tag="JN334_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HAY7438218.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            12485..13699
                     /gene="iscS"
                     /locus_tag="JN334_002077"
     CDS             12485..13699
                     /gene="iscS"
                     /locus_tag="JN334_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAY7438219.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            13727..14113
                     /gene="iscU"
                     /locus_tag="JN334_002078"
     CDS             13727..14113
                     /gene="iscU"
                     /locus_tag="JN334_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HAY7438220.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            14130..14453
                     /gene="iscA"
                     /locus_tag="JN334_002079"
     CDS             14130..14453
                     /gene="iscA"
                     /locus_tag="JN334_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HAY7438221.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            14549..15064
                     /gene="hscB"
                     /locus_tag="JN334_002080"
     CDS             14549..15064
                     /gene="hscB"
                     /locus_tag="JN334_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HAY7438222.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            15081..16931
                     /gene="hscA"
                     /locus_tag="JN334_002081"
     CDS             15081..16931
                     /gene="hscA"
                     /locus_tag="JN334_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HAY7438223.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            16933..17268
                     /gene="fdx"
                     /locus_tag="JN334_002082"
     CDS             16933..17268
                     /gene="fdx"
                     /locus_tag="JN334_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HAY7438224.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            17280..17480
                     /gene="iscX"
                     /locus_tag="JN334_002083"
     CDS             17280..17480
                     /gene="iscX"
                     /locus_tag="JN334_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HAY7438225.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            17539..18822
                     /gene="pepB"
                     /locus_tag="JN334_002084"
     CDS             17539..18822
                     /gene="pepB"
                     /locus_tag="JN334_002084"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HAY7438226.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            18964..19740
                     /gene="sseB"
                     /locus_tag="JN334_002085"
     CDS             18964..19740
                     /gene="sseB"
                     /locus_tag="JN334_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HAY7438227.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(20559..>21038)
                     /gene="sseA"
                     /locus_tag="JN334_002086"
     CDS             complement(20559..>21038)
                     /gene="sseA"
                     /locus_tag="JN334_002086"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HAY7438228.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
BASE COUNT         5040 a         5324 c         5718 g         4956 t
ORIGIN      
        1 acgggagcag tggctggcgg gtgagttttg cgagcagcta ccgcaggaac tggattttaa
       61 ccagctcgcg ccgttgccgc gtcagtgggt gaacaatggt tttgccggat ggaatggtca
      121 ggcccgcatc gagcagccgc aggaaggtta tgccatcatc atggaaacga cgccacctgc
      181 accgtgttat ttcatctttg tttccgaccc tgcgtttgat aaaggatatg cgtttgattt
      241 cttctgtctg gaaccgatga gccatgcgcc ggatgagcat catcgcccgg aagggggtga
      301 tctcattgcg ctagcaccag gggaatcaac aacctcagag atgtcgttgc gggtagagtg
      361 gctgtaattt ataaaatgcc ggataaggcg ttcacgccgc atccggcatc tttcctaacg
      421 ccgatcgaga gaaattgccc ccggtccggt aattgccaat agcaagaacg cgccagcgat
      481 actgacattc ttccagaaat taatcatatt tggcccaacc gcatcgccgg tcatatccca
      541 gtaatggtga ccaatcaccg ccgtacccag cgtgtagaaa ataaacagca ccgccagcgg
      601 acgggtgaaa aagccaagca cgattaatat cgcggcgggc acttccataa ctaccgcaat
      661 aatcgctgcc agcatcggca ttggcgcgcc caacgaggcc atatattgga ccgtaccgtc
      721 aaagcccatc attttgggaa aaccaaaaat aatgaaaatt aagaccacgg cgatacgggc
      781 aattaataac aaaacggggc gggcagctcc aaaatcaaaa taacgtagtg tgttcataac
      841 caatcccttt gcaagataat gtgatttaaa cgtaatacac actcttgcac ctcgccacgc
      901 cccaggcatt gagatattcg ctaattatcc tgattacagt gatttaagcg cgatgttctc
      961 atctatcagc agtttcgcat caaacgtttt gccgctctgg atccatttgc gaattgggcg
     1021 aatctcacgg ccctgattca gcgtgaccgc accgataagc acgccgtttt gcagattaaa
     1081 ccaaatcgcc ttctgagttt ccgggttgcc acgacaaagc cagtcatcgc cacgcatatc
     1141 gccaataaac tgtaagttat cactgtactg atcgctccag aaccacggcg gcggcagtag
     1201 cggtagcggt agccctaaca ttgcggcagc ggcaatttgc gcctggttat tggcgttttc
     1261 ccagctttcg cagcggtgta gtgcaccatt atcaagacga gtgattgcca catcgccacc
     1321 ggcaaagatc gcgggatcgc aggtgcggca agcctcatca atgacaatgc cattggcagt
     1381 atcaaggttg gcctcgcgag ccagttgctc gttggcgctg ataccaatac cgtaaatcac
     1441 cacatcagcc tgaagcgttt ccccactttg cagcgtcagt tctacttttt caccatcgac
     1501 cacatgttca atggcattat tgagcagaat gcgcacacca gcctgctgat ggcgctgtaa
     1561 aagatagcgt tgcacgggcg gtggtgcatt acggcccatg acggttgccg ccagttcaat
     1621 cactgtcacc ttacatctgc gctgcgctgc gctgcgctgc gtggcgctgg cagccagttc
     1681 cagaccaata gttccggcac cgacaatcac gactgaccgt tcgggctgca gaacttctcg
     1741 cagtctggcg gcatcaccgg catggtgtag ggtaaagcag cgttctccca gtgcatcaag
     1801 caacggcagc ggtcgagctg ccgcgccggt tgctataaaa agctgatccc agtgccagct
     1861 ttcgccgttg gttaacacta actctcgtgt gtcgcgaccc agcgttttga tggttacacc
     1921 ggaatgcaga tgaacattgt tttcctgcca ccagttagcg ggtaacacct gctgtaactg
     1981 cggggaatct tccagcaaca tggatttcga gagtggaggt cgttcataag gaagatgtcg
     2041 ctcatcggaa aacagatgca gctcaccggt gaacccttgc tggcgtagcg aggccgcagc
     2101 cattgccgcc gcttgcccgc caccgacaat aatgatcgtt ttttctttca tcgtgcttcc
     2161 ttaaagatcc agcccagccg ctacgtggcg aatgccgcga atcgctaaac ccgcatcagc
     2221 gttgatcatc acaccgctta atgcgcgatt attgcgccgc gatgccaaca tcacatacgg
     2281 ccccgtaaaa tccgccggtt gcgggaaaaa ttgcagcggt aaaatggcgg caattttctc
     2341 cggcgtcaga gactgcatta tcgaggtttc actttgcccg agcgcctgtg ggccgcgcag
     2401 gtcgctggcc ataccacacg ggccgacgcc attcacccgc actttcggtg ccagttcata
     2461 agccagttgg cgaataagtc cggttgcggc atgtttactg gtggtgtaca gcgggccacc
     2521 gccgccagga taccaggcgg catttgacag tgtgaaaatc atgctgcctt cactggcgat
     2581 taacgccgga gcgcaggctt ttgcgcccag caggtaaccg agtacgttga cgttaaacag
     2641 ctcgtggaag ccggtttcga gcgtctctgc gggagtatta accagtgagg cattgtgatc
     2701 ccagatgcct gcattgccga taaaacaatc cagcttgccg gaacgagtca ggatctgatc
     2761 gaccgcgcgt tgataatcgg cataacaggt cacgttacct tccaccgcca gaatatgttc
     2821 gccaaatcgc tgacgcagac tggcgacttt tgccgccgac agttccagcg tggcaacttg
     2881 cgcgccttct tcgataaatc gctcgaccag cgccagccct aatcccgatc cgccgccggt
     2941 aataaaaatg gactcgttat gcagatcgct catggctgcg cctccgggaa gtcgatgaaa
     3001 atgtcaccac cttcaacgtg tactggataa gtggtgagcg gatcggtggc gggcaggcat
     3061 aacgctttcc ccgttttcag gcaaaaactg gcggcgtgta gcgggcactc caccgtggcg
     3121 tcatcttcca gatacccttc tgacatcgac gcattaccat ggctgcaacg atcgttaatg
     3181 gcataaaact cgccgcccac gttgaacaag gcgatgacgg gcgaggtatc tatccggaga
     3241 gcctcaccct ccggcacatc cgcaacggga cacgcataaa ttcgattcat cagaacagta
     3301 cactcaggtt atgggaagtg atcaccgctt gatccaggac gatatcccgt tccagcaagc
     3361 gccagttgtc atcttccaga cggcgaactt tgtcgaaacg cgttccaaca tagaatgttt
     3421 catcgcgctc tttttgtgcc cgataaagta gataattcac cctcacggca aatacgtttg
     3481 ggatgtcggt ttcgcttacc tggcagttgc tgattaagtg acgagtgcgt gacggcggct
     3541 cttctgccca ggccatgccc gtttccagac gggcgattcg ccgctccagc tggtctttgg
     3601 tgtcattgaa aatccaggtc gtcggcggct ggacgccttt gcgacggtcg cgtgtttgcg
     3661 cgttaaccgt ggtgcgcatg gtgtaacgaa tctcttcgtc gagctgtgcc aaccagtcac
     3721 gaaatttcca gtcatccagc agactggctt cgtgaaagag aaactggcta atgcggtgat
     3781 gtaactccag tgaaacttgc gcactcattt catcacctcc tgctggtaag cggcggtttt
     3841 atcgagcact tcctgccagc tttcgctact cagaagatcg gcccagcgtt ggtacattcc
     3901 acgagcggca gtttctgaaa agatatagtt agtaatgcca ggaatgccgt cgtcgcggcg
     3961 cttttcctga ccaagcccca tttccagaca cagtttgctg ttgcgggcgc ggtggccttt
     4021 aagcaatttc tggatttcac accagttctc cgagtcatcc tgctcgagaa aaccagcagg
     4081 accaaaagca cgagtggcgc tgttttcaaa agcggcttta acttcatcgg aggcggcttt
     4141 gtcagtaata cagaacgccc acacttcaac ttgatcaggg ccgcgcggat gccagacgcg
     4201 gagtgtggca gtgccgttta gccatgaaag cgtggggaat atattgttat gacccgccag
     4261 gcgcagggcg cgaacttcac ctaaacgttg ttctgcttcg gcataggttt cgcggtaata
     4321 gcttgaaact gcgccatcga cccagacatt agcatccggt ttttcagtaa agaagaaacc
     4381 gctaccgtga ccgtcctgac caaattgcag cgcatctttg gcggtttccc acaccgggcg
     4441 ggcggtttgt ccatcaccga ggcgcttatc gctgccatca tctttcgccc ctaatacctg
     4501 aacggcagaa gcatggctga acagagcatg atactggtca ctggcgaact gctctgccgg
     4561 gaatttccag ttacagttga tcacccactt ttgtacgccg ccgacaattt cggtgccgcc
     4621 ttcgcgacga tccagcatgc catccagata ccaggcaatg tcacccaggt aatcatgcag
     4681 gcccggtgcg ctggtatccc agttgccaaa aatcagtcct ttataactct ccacacaagg
     4741 aacttcgttt agcccccagt gggatttaca caacccttgt gggtaggcgc gaggttccag
     4801 cggtacatcg atcaactcgc cgttaatgcc ataagaccag ccgtgatacg ggcaggtaaa
     4861 ggcgcgactg ttgccacaat ctgcataact cacacgcatg gcccggtggc ggcattggtt
     4921 gagaaacgcc ttgatgctgc cgtctttctg gcgcaccacg acaactgcat cttctcccat
     4981 gtaggtgtta aagaaatcac cgggttttgg gatctggctt tcgtgggcga gaaatagcca
     5041 gcaacgaccg aaaatacgct caagctccag ttggtaaatg tccgggtcgg tataaatacg
     5101 cggagtgacc cgaccgtttt gggtgtcaat cagttggtaa atgttcaaat ctgagggtgt
     5161 ggtcatattt tttaatcctg aaagataaga tgtattgacc ttctattttt gtgccataag
     5221 gatgttgctg gttggttgct aatatgtgaa atagttaatt tctaccagct gagacgtttt
     5281 ttcgataaca aggggaaggt gatggaacta cggcatttac gctatttcgt cgcagtggcg
     5341 caggcactga actttacccg tgcggcggaa aaactgcata cctcacagcc ttcgttaagc
     5401 agccagatcc gcgatcttga aaactgtgtc ggtgttccgt tactggtgag ggataagcgc
     5461 aaagtcgcgt tgacggcggc gggagaatgt tttctccagg acgcgctggc aatcctcgaa
     5521 caagcggaaa atgccaaatt acgggcgcgg aaaattgttc aggaagacag acaattaacc
     5581 attggctttg tgccatcggc ggaagtgaat ttattgccaa aagtattacc gatgtttcgt
     5641 ctcagacagc cagacacctt gattgagctg gtgagtttaa tcaccacgca acaggaggaa
     5701 aaaattcgcc gtggtgaact cgacgtcggc ttaatgcgcc atcctgttta tagcccggag
     5761 attgattatc tggagctttt tgacgaacca ttagtggtgg tgttaccggt tgatcaccct
     5821 ttagcacatg aaaaagagat caccgccgcg caactggatg gcgtgaattt cgtcagtacc
     5881 gatccggcgt attccggttc gcttgcgccg atcgttaaag cgtggtttgc gcaagaaaac
     5941 agccagccaa atatcgtcca ggtggcaacg aatattctgg tgaccatgaa tctggtgggc
     6001 atggggctgg gcgtcacttt gatacccggt tatatgaata attttaatac cgggcaggtt
     6061 gtttttcgtc ccattgccgg aaatgtgcct tccattgctt tactgatggc gtggaagaaa
     6121 ggggagatga agctggcatt acgcgatttc atcgccattg tgcaggaacg tttggcaagc
     6181 gtaacggcat aattacgcat tatttttgca acatgcgtcg cggatcgcag ttttttagtg
     6241 cttgtgaact ggcgttacag acatcgggaa gcaggctcga atattcttat ttttcaactt
     6301 gcaccaacat tcgattaacc ccagactaac gcctcctgac gggagggact catggttttg
     6361 caatccacgc gctggttggc gctcggctat ttcacatact tttttagtta cggcattttt
     6421 ttacctttct ggagcgtctg gcttaaaggg attggtttaa cgccagaaac catcggcctg
     6481 ttattggggg caggtctggt tgcccgtttt ctcgggagtt tgctcatcgc gccccgcgtc
     6541 agcgatcctt cccgcctgat ttccgccttg cgcgtgctgg cactgctgcc acttctcttt
     6601 gctgtcgcct tctgggcggg ggcgcacgta gcgtggctga tgctggtgat gattggcttt
     6661 aacctctttt tctcaccgct ggtaccgttg accgatgcac tggcgaatac gtggcaaaag
     6721 cagttcccgc ttgattacgg caaagtgcga ctgtggggct cggtggcgtt tgtcattggc
     6781 tcggcgctga cgggcaaact ggtcactatg tttgattatc gggtgatcct cgtgctgttg
     6841 acgttgggcg tggcatccat gctgctcggc tttctcatcc gtccgacgat tcagccacaa
     6901 ggggcaagcc gccagcagga gagcaccggt tggtctgcgt ggttggcgct ggttcgccag
     6961 aactggcgct ttctggcctg cgtttgttta ttgcaggggg cacatgcggc ctattacggt
     7021 tttagcgcca tttactggca ggcagctggc tactcggcct cggcggtggg gtatttgtgg
     7081 tcgctgggcg tggtggcgga agtcattatc tttgcgctga gtaataaact tttccgccgt
     7141 tgtagtgctc gcgatatgct gttgatctcg gcgatttgcg gcgtagtgcg ctggggcatt
     7201 atgggagcaa ctacggcgtt gccgtggttg atagtggtgc aaattctgca ttgcggcacc
     7261 ttcacggtct gccacctggc cgccatgcgt tatattgctg ctcgccaggt tagcgaagtc
     7321 atccgtttac aggcggttta ctctgccgtc gcgatgggcg gcagtatcgc tatcatgacc
     7381 gttttcgccg gtttcctgta tcaatatctg ggccacggcg tgttctgggt aatggcgctg
     7441 gtggcgcttc cggcaatgtt tttgcgcccg aaagttgttc cctcatgctg attcgagcat
     7501 tttgcggatc tgctcctgtt gatgtgttgt cagcgtcagg tccgtataga tcagcggtgg
     7561 tgaaaagagc ggtaacggca tggtataagg cgcaataatc agtgccgcgc cacgcggagc
     7621 gcctgtctgc aaaaatgcct ttaccgacat atgcttaatg ttgagcggca gtaacgttag
     7681 ttcgcgtaac tgctgctcaa tctgcgcttc tcgctcgcta tcattcccgg tcagtagaat
     7741 aatctgtttt tcatgcagat cgttttcctg cattagccag gcaccgaaaa tcaccgcgac
     7801 cagaccactt tcctcatcag aaagatggac gccgtattca ctttcaaatc cggccagcgc
     7861 cgcgcgggtg gtgcgtacca ggcgtggata cagtctggcg aactcttccg gcagggtatt
     7921 atcaataccg atggcaaaca aactgcgatt taacgcctgg gcgaggtgga tataaagctg
     7981 atcacataac ccctgttcat cataaaaacg aacatttccc agctcacgaa aatggtttac
     8041 cagacgtttg atagactgac gcaattgtct gtcccgctga tgcgcatccc gcagtggatc
     8101 gggaacccgc aacatggaaa aaagtaatgc cataaacagt ggctcatcag gcggtacatg
     8161 ctggagcgcc cgacgctgcc agtggcgtcc aatttcctgc gctacctgaa attcaaggca
     8221 ggattccgcc cagcgacgtt ggagcggatt aaactgaggc gtaattccag cgtggtgctg
     8281 cagcaaacaa tattgcagat acagacacag gaaatgaata tcgcgcgatt caaagcgttt
     8341 ttgcagccgt cgggagcaga gattcactaa cgcttgtaga ttggtatcgt cgtaaaagtt
     8401 acgcgcaatt ccgcgccgct tcagttcact cttcagggcg ggggtgaatt ggctggtaat
     8461 aaatgacgga cacagacgga aaccacgtcg tagccagtga aataaacaca aacgttggtt
     8521 aagcactgta ccttcaaccc ggtagctacc gtcataacca gtcgtcagtg tgagttgatg
     8581 atagcgcagg atctcccgtc ctgtttcgct gatatcaaga ctggcaatat catcatccac
     8641 accattaagc tcgctgaagg ttgccatggt ggcggtcaac cccggctgaa agagcgtcag
     8701 caagatctgg cagcggcgct ggggagccga aaggacagat ggtggagcaa gcgtaggcat
     8761 catctgtaga tctcccataa gcataattga taagaatagc taaagtttgc tcagggaaat
     8821 ggcgaaggga atgctaatca tcagaaatgt tggcaagcgt cacagaattt ttctcatttt
     8881 aaactgttct gaccagtgga ttagcatgtc ttaaggtggt tttgtgaatg attatcgtgc
     8941 tagcgatcat gggttagttc tgcggattca acgcgctaag gataaagtcc accatctgtt
     9001 cgcgataaac attgccgtga cgatcggaaa aagcatcaat gtgttcgcca tccgggatta
     9061 ggatcaaccg ttttggctct tttgccaggc tatacaactt ttcgctgtgc tgccatggga
     9121 taacgtgatc ggctttaccg tgaatgagta aaagcgggat cgggctgacg ctggcgatgt
     9181 aattttcgcc gctgtaactc tcatcaagta agtagccact gccggggatc atctggttgg
     9241 cgatggttgc ataagaggca aatgtggagt cgaggatcac cgcacgtatg ccttcacgat
     9301 caccctgacc aataacagca agaatattcg ccccgccaat actctgcccg aacagcacca
     9361 gctgttgtgg gtttacatca ctgcgatggc gcaccacatt gatggcgctt tgcgtatcgt
     9421 ccagcaatcc ggcctgggac ggagtgcctt ttgatttacc aaacccgcga taatcaaaca
     9481 taaaaacgtt gaaattacgc tcaggtaacc aactgaccag cggccagtgg gcggacatat
     9541 ttccggcatt gccgtgagca tgaatgatgg ttgcgatggc gttgtcagca gggcccgtcg
     9601 aagaaggaat aaaccagcct tgcagacgag taccgtcctt agccgtaaat tcgacggatt
     9661 ccgccgacca ggggtcggga ccgtaaattt tatcgtcagg ataatagaag aagttgatgg
     9721 caacgcgcgg cactaaataa acgcagaagg caacaacaaa gagaataaac agaattttgg
     9781 gaacgcgttt gttcactggc agtgccataa cctttccttg cgacttcttt atcatccatt
     9841 aatcggggaa agatggtggg agagtgacga aagaaagtaa agcggtgagg aagtatttcg
     9901 gaatagcgcc tgaaaggcga gggcgggtga gtgatatcac ccgcctgagt cattaatgct
     9961 tcagagcgtc gcttaactcg tcacgcatgt tcgccagtat ggctttcaca acacgcgggt
    10021 taccagcaac gatgttaccg gtcagcatat agttatgacc accggtgaag tcgctgacga
    10081 tgccgcccgc ttcacgaacc agcagctcgc ctgccgcgaa atcccacgga cgcagaccga
    10141 tttcaaagaa accgtcaaca cgacccgcag cgacgtaagc cagatccagc gccgcagaac
    10201 cggtacgacg gaagtctgca cattcgttga acagcttgcc gacgatgttg atgtaggtag
    10261 tggcgtactg ttttgctttg aacgggaagc cggtcgccag aatggtaccg tcgagatcgc
    10321 gagcggtgct accgcgcaga cggtagccgt tcagctgtgc gccttgaccg cgagtggcgg
    10381 tgaacagttc gttacgcata ggatcgtata ccacagcaac ttcggtgcgg cctttgatac
    10441 gaacggcgat agataccgcg aagtgcggca gacgtttgat aaagttggta gtaccatcca
    10501 gtggatcgat aacccattga acatcctgat cagtaccttc aagttcaccg ctttcttcgg
    10561 tgatgatggt gtgctgtggg taagatttac gaatcgtgtc gataatcacc gcttcggcag
    10621 ctttatctac gttggtcacg aaatcgttac tgcctttctg gctcgcttct acagcgtccg
    10681 gggtttcata gtttttggca attaaattac ccgccttgcg cgctgcgcgc acggcgatgt
    10741 tcagcatcgg atgcatcggt ctctctcact ggatgttaaa gaacgggaaa acggcgcaga
    10801 gtatagcagc gagatcggca tctgtcttcg ttttatgata agatgcgtga ataatcttta
    10861 gatgaagaaa gacaatgctg caaaatattc gaattgtgct ggtggagacg tcacacaccg
    10921 gcaatatggg ttctgttgcc cgtgccatga aaacaatggg attaaccaat ctgtggctgg
    10981 ttaatccact ggtgaaaccc gactcccagg cgattgccct ggctgcaggg gccagcgatg
    11041 tgattggtaa tgctcacatc gtcgatacgc tcgacgaagc gttagccggt tgtagcctgg
    11101 tggtgggcac cagcgcacgc tcccgcacgc tgccgtggcc gatgctcgac ccgcgcgaat
    11161 gcggtctgaa aagcgtcgct gaagcggcaa ataccccggt ggcgctggtg tttggtcgtg
    11221 agcgcgtcgg cttgaccaat gaagagttgc agaaatgcca ttatcatgtc gcgattgcgg
    11281 caaacccgga atacagttcg ctgaacctgg caatggcggt tcaggttatt gcctatgaag
    11341 tgcgtatggc ctggctggca actcaggaaa atggcgagca ggtcgagcat gaagagacgc
    11401 cgtatccgct ggtcgatgat ctggagcgtt tttatggtca tctggaacaa acgctgctgg
    11461 caaccggttt tatccgtgaa aaccatccgg ggcaggtgat gaataaattg cgccgtctgt
    11521 ttacccgtgc gcgcccggaa agccaggaat tgaatatcct gcgcgggatt ctggcttcta
    11581 ttgagcagca gaataaaggt aacaaggccg aataacagtg gttgcctgat gcgacgctta
    11641 acgcgtctta tcaggcctac aatgtacaga atcgtaggtc ggataaggtg ttcacaccgc
    11701 atccgacaac aggtacaaac gccacgataa aaaaatggca ctgaaggtta aatacccgac
    11761 taaatcagtc aagtaaatag ttgaccaatt tactcgggaa tgtcagactt gaccctgcta
    11821 tgcaataccc ccacttttac aataaaaaac cccgggcagg ggcgagtttg aggtgaagta
    11881 agacatgaga ctgacatcta aagggcgcta tgccgtgacc gcaatgcttg acgttgcgct
    11941 caactctgaa gcgggcccgg taccgttggc tgatatttcc gaacgtcagg gaatttccct
    12001 ttcttatctg gaacaactgt tttcccgtct gcgtaaaaat ggtctggttt ccagcgtacg
    12061 tggaccaggc ggtggttatc tgttaggcaa agatgccagc agcatcgccg ttggcgaagt
    12121 gattagcgcc gttgacgaat ctgtagatgc cacccgttgt cagggtaaag gcggctgcca
    12181 gggcggcgat aaatgcctga cccacgcgct gtggcgtgat ttgagcgacc gtctcaccgg
    12241 ttttctcaac aacattactt taggcgaact ggttaataac caggaagtgc tggatgtgtc
    12301 tggtcgtcag catactcacg acgcgccacg cacccgcaca caagacgcga tcgacgttaa
    12361 gttacgcgct taataaaaag aattcagaat caggccggag tgctaaatac tccgtaaaca
    12421 cggtcgtaca tccagccggt agcctgattc cttgcattga gtgatgtacg gagtttatag
    12481 agcaatgaaa ttaccgattt atctcgacta ctccgcaacc acgccggtgg acccgcgtgt
    12541 tgccgagaaa atgatgcagt tcatgacgat ggacggaacc tttggtaacc cggcctctcg
    12601 ttctcaccgt ttcggctggc aggctgaaga agcggtagat atcgcccgta atcagattgc
    12661 cgatctggtc ggcgctgatc cgcgtgaaat cgtctttacc tctggtgcaa ccgaatctga
    12721 caacctggcg atcaaaggtg cagccaactt ttatcagaaa aaaggcaagc acatcatcac
    12781 cagcaaaacc gaacacaaag cggtactgga tacttgccgt cagctggagc gcgaaggttt
    12841 cgaagtcacc tacctggcac cgcagcgtaa cggtattatc gacctgaaag aactcgaagc
    12901 ggcgatgcgt gacgacacca tccttgtttc catcatgcac gtaaataacg aaatcggcgt
    12961 ggtgcaggat atcgcggcta tcggcgaaat gtgccgtgct cgtggcatta tctatcacgt
    13021 tgatgcaacc cagagcgtgg gcaaactgcc aatcgacctg agccagttga aagttgacct
    13081 gatgtctttc tccggtcaca aaatctatgg cccgaaaggt atcggtgcgc tgtatgtgcg
    13141 tcgtaaaccg cgtgtacgca tcgaagcgca aatgcacggc ggcggtcacg agcgcggtat
    13201 gcgttccggc actctgcctg ttcaccagat cgtcggaatg ggcgaagcct atcgcatcgc
    13261 aaaagaagag atggcgaccg agatggaacg tctgcgcggc ctgcgtaacc gtctgtggaa
    13321 cggcatcaaa gatatcgaag aagtttacct gaacggtgac ctggaacacg gtgcgccgaa
    13381 cattctcaac gtcagcttca actacgttga aggtgagtcg ctgattatgg cgctgaaaga
    13441 cctcgcagtt tcttcaggtt ccgcctgtac gtcagcaagc ctcgaaccgt cctacgtgct
    13501 gcgcgcgctg gggctgaacg acgagctggc acatagctct atccgtttct ctttaggtcg
    13561 ttttactact gaagaagaga tcgactacac catcgagtta gttcgtaaat ccatcggtcg
    13621 tctgcgtgac ctttctccgc tgtgggaaat gtacaagcag ggcgtggatc tgaacagcat
    13681 cgaatgggct catcattaat cggtatcgga atcaggagaa tttataatgg cttacagcga
    13741 aaaagttatc gaccattacg agaatccgcg taacgtgggt tcctttgata acaacgacga
    13801 gaacgtcggc agcggcatgg tgggtgcacc ggcctgtggc gacgtaatga agttgcagat
    13861 taaagtcaac gatgaaggta tcattgaaga cgcgcgtttt aaaacttacg gctgcggttc
    13921 cgctatcgct tccagctccc tggtcaccga atgggtgaaa gggaagtctc tcgacgaagc
    13981 gcaggcgatc aaaaacaccg atattgctga agaacttgaa ctgccgccgg tgaaaattca
    14041 ctgttctatt ctggcagaag acgcgatcaa agccgccatt gcggactata aaagcaaacg
    14101 tgaagcaaaa taagagttga ggtttggtta tgtcgattac actgagcgac agtgcagcag
    14161 cgcgagtaaa tacctttctg gctaaccgcg gcaaagggtt tggcctgcgt ctgggcgtga
    14221 gaacctccgg atgttcaggt atggcttatg tactggaatt tgttgacgaa ccgacgccgg
    14281 aagacatcgt gtttgaagac aaaggcgtga aagtcgtggt cgatggcaaa agcctgcaat
    14341 ttctggacgg tacgcagctg gacttcgtaa aagaaggcct gaatgaaggg tttaaattca
    14401 ccaacccgaa cgttaaagat gagtgtggtt gcggcgaaag cttccacgtt tgatgcgcat
    14461 accgataacc ccaccgtggt cgcctgcgcg tggggtttgt tttacctgat tcgccgttat
    14521 cgcggcggat cgcagccctg agaatgttat ggattacttc accctctttg gcttgcctgc
    14581 ccgttatcaa ctcgataccc aggcactgag cctgcgtttt caggatctac aacgtcagta
    14641 tcatcctgat aaattcgcca gcggaagcct ggcggaacaa ctcgccgccg tacagcaatc
    14701 tgcaaccatt aaccaggcct ggcaaacgct gcgtcatccg ttaatgcgcg cggaatattt
    14761 gctttcttta cacggttttg atctcgccag cgagcagcat actgtgcgcg acaccgcgtt
    14821 cctgatggaa cagttggagc tgcgcgaaga gctggacgag atcgaacagg cgaaagatga
    14881 agcgcggctg gaaagcttta tcaaacgtgt gaaaaagatg tttgataccc gccatcagtt
    14941 gatggttgaa cagttagaca acgaggcgtg ggacgcggcg gcggatacgg tgcgtaagct
    15001 gcgttttctc gataaactgc ggagcagtgc cgaacaactc gaagaaaaac tgctcgattt
    15061 ttaatttctg gaagctaaac atggccttat tacaaattag tgaacctggt ttgagtgctg
    15121 cgccgcatca gcgtcgtctg gcggctggta ttgacctggg cacaaccaac tcgctggtgg
    15181 cgacagtgcg cagcggtcag gccgaaacgt tagccgacca tgaaggccgt cacctgctgc
    15241 catctgttgt tcactatcaa cagcaagggc attcggtggg ttatgacgcg cgtactaacg
    15301 cagcgcttga caccgccaac accattagtt ctgttaaacg cctgatgggg cgctcgctgg
    15361 ctgatatcca gcaacgctat ccgcatctgc cttatcaatt ccaggccagc gaaaacggcc
    15421 tgccgatgat tgaaacggcg gcggggctac tgaacccggt gcgcgtttct gcggacatcc
    15481 tcaaagcact ggcggcgcgg gcaactgaag ccctggcagg cgagctggat ggtgtagtta
    15541 tcaccgttcc ggcgtacttt gacgatgccc agcgtcaggg caccaaagac gcggcgcgtc
    15601 tggcggggtt gcatgttctg cgcttactta acgaaccgac tgctgcggct atcgcctacg
    15661 ggctggattc cggtcaggaa ggcgtgatcg ctgtttatga cctcggtggc gggacgtttg
    15721 atatttccat tctgcgctta agtcgcggcg tgtttgaagt gctggcaacc ggcggtgatt
    15781 ccgcgctcgg cggcgatgat ttcgaccatc tgctggcgga ttacattcgc gagcaggcgg
    15841 acattcctga tcgtagcgat aaccgcgttc agcgtgaact gctggatgcc accattgcag
    15901 ccaaaatcgc gctgagcgat gcggactccg tgaccgttaa cgttgcgggc tggcagggcg
    15961 aaatcagccg tgaacaattc aatgaactga tcgcgccact ggtaaaacga accttactgg
    16021 cttgtcgtcg cgcgctgaaa gacgcgggtg tagaagctga cgaagtgctg gaagtggtga
    16081 tggtgggcgg ttctactcgc gtgccgctgg tgcgtgaacg ggtaggcgaa tttttcggtc
    16141 gtccaccgct gacttccatc gacccggata aagtcgtcgc tattggcgcg gcgattcagg
    16201 cggatattct ggtgggtaac aagccagaca gcgaaatgct gctgcttgat gtgatcccac
    16261 tgtcgctggg cctcgaaacg atgggcggcc tggtggagaa agtgattccg cgtaatacca
    16321 ctattccggt ggcccgcgct caggatttca ccacctttaa agatggtcag acggcgatgt
    16381 ctatccatgt aatgcagggt gagcgcgaac tggtgcagga ctgccgctca ctggcgcgtt
    16441 ttgcgctgcg tggtattccg gcgctaccgg ctggcggtgc gcatattcgc gtgacgttcc
    16501 aggtcgatgc cgacggtctt ttgagcgtga cggcgatgga gaaatccacc ggcgttgagg
    16561 cgtctattca ggtcaaaccg tcttacggtc tgaccgatag cgaaatcgct tcgatgatca
    16621 aagactcaat gagctatgcc gagcaggacg taaaagcccg tatgctggca gaacaaaaag
    16681 tagaagcggc gcgtgtgctg gaaagtctgc acggcgcgct ggctgctgat gccgcgctgt
    16741 taagcgccgc agagcgtcag gtcattgaca atgctgccgc tcacctgagt gaagtggcgc
    16801 agggcgatga tgttgacgcc atcgaacaag cgattaaaaa cgtagacaaa caaacccagg
    16861 atttcgccgc tcgccgcatg gaccagtcgg ttcgtcgtgc gctgaaaggc cattccgtgg
    16921 acgaggttta atatgccaaa gattgttatt ttgcctcatc aggacctctg ccctgatggc
    16981 gctgttctgg aagctaatag cggtgaaacc attctcgacg cagcgctgcg taacggtatt
    17041 gagattgaac acgcctgtga aaaatcctgt gcttgcacca cctgccactg catcgttcgt
    17101 gaaggttttg actcactgcc ggaaagctca gagcaggaag acgacatgct ggacaaagcc
    17161 tggggactgg agccggaaag ccgtttaagc tgccaggcgc gcgtcaccga cgaagattta
    17221 gtggtcgaaa tcccgcgtta cactatcaac catgcgcgtg agcattaaca gaggttagta
    17281 tgggacttaa gtggaccgat agccgcgaaa ttggcgaagc actgtacgat gcgtatcccg
    17341 atcttgatcc gaaaacggtt cgattcaccg atatgcatca gtggatttgc gatctggaag
    17401 atttcgacga cgacccgcag gcatccaacg agaaaatcct cgaagcgatt ttgttagtct
    17461 ggctggacga agccgagtga atgaacgggc tgcctggggg tagcccgttt tgctgttaaa
    17521 cgaataagga taactaaaat gacagaagcg atgaagatta ccctctctac ccaacctgcc
    17581 gacgcgcgct ggggagaaaa agcaacttac agcattaata atgacggcat taccctgcat
    17641 ttgaacgggg cagacgatct ggggctgatc cagcgtgcgg cccgcaagat tgacggtctg
    17701 ggcatcaagc atgttcagtt aagcggtgaa ggctgggatg cggatcgctg ctgggcattc
    17761 tggcaaggtt ataaaggccc gaaaggcacg cgtaaagtgg agtggccgga tctggacgat
    17821 gcccagcgcc aggaactgga taaccgcctg atgatcatcg actgggtgcg tgacaccatc
    17881 aacgcaccgg cagaagaatt gggaccatcg caactggcac agcgtgctgt tgatctgatc
    17941 agcaacgtcg cgggcgatcg tgtgacttat cggatcacca aaggcgaaga tctgcgtgag
    18001 caaggttata tggggctgca cactgtcggg cgcggttcag aacgttctcc ggtattgctg
    18061 gcgctggatt acaacccaac tggcgataaa gaagcaccgg tgtacgcgtg cctggtaggt
    18121 aaaggtatca cttttgactc cggcggctac agcatcaaac agactgcgtt tatggactcg
    18181 atgaagtcgg acatgggcgg cgcggcaacg gttaccgggg cgctggcatt tgccattatg
    18241 cgcggactga acaagcgcgt gaagctgttc ctctgctgtg cggataacct gattagcggc
    18301 aatgcgttca agctgggcga tatcatcacc tatcgcaacg gtaaaaaagt tgaagtgatg
    18361 aacactgatg ccgaagggcg tctggtgctt gccgatggtc tgattgatgc cagtgcgcag
    18421 aaaccggaaa tgatcattga tgcggcgacc ctcaccgggg cggcgaaaac tgcgctgggt
    18481 aatgattatc acgcgctgtt cagttttgac gatgcgcttg ccggtcgttt actggcgagt
    18541 gcctcacaag agaacgaacc attctggcgt ctgccgctgg cggaattcca ccgcagccag
    18601 ctgccgtcta actttgccga actgaacaat accggaagcg cggcgtatcc ggcaggcgcg
    18661 agcacggcgg cgggcttcct gtcgcacttt gttgagaact atcagcaagg ctggctgcat
    18721 atcgactgct cggcgactta ccgtaaagcg ccggttgaac agtggtctgc gggcgctacg
    18781 ggacttggtg tgcgcacgat agctaatctg ttaacggcgt aaactaatat gccggatgcg
    18841 gcgtaaacgc cttatccggc ctacaaattc tgcaaattca acaaattgcg aatcccttgt
    18901 aggcctgata agcgtagcgc atcaggcatt tgcgccccga acgttgaatg aattgtggtt
    18961 cttatgtccg aaacaaaaaa cgaacttgaa gacctgctgg aaaaagcagc aaccgaaccg
    19021 gcgcaccgcc cggccttttt ccgtactcta ctggaatcca ccgtctgggt gcctggtacg
    19081 gcggcgcagg gcgaggctgt ggttgaagat agtgcgcttg atttacagca ctgggaaaaa
    19141 gaagacggca ccagcgtcat tccttttttc acctcgttag aagcgcttca acaggcggtt
    19201 gaagacgaac aggcatttgt cgtaatgccc gttcgcacat tgtttgagat gacccttggc
    19261 gaaacgctct tccttaatgc caaactgccg accggtaaag aatttatgcc gcgtgaaatc
    19321 agtttgttga ttggtgaaga gggaaatccg ctgagcagcc aggaaatcct ggaaggcggt
    19381 gaatcgctga tattatcgga agtcgcagag ccgccagcac aaatgattga ttcactcacc
    19441 accttattta aaaccattaa gccggtgaag cgtgctttta tttgttcaat taaagagaac
    19501 gaagaggcac agcctaattt acttattggc attgaagccg atggtgatat cgaagaaatt
    19561 attcaggcga cgggaagtgt agcgaccgat acattacctg gcgatgaacc aatcgatatt
    19621 tgtcaggtga aaaaagggga aaaaggaatt agccacttta ttaccgaaca tattgcgcca
    19681 ttctatgaac gtcgctgggg tggttttttg cgtgacttta aacaaaaccg gataatctaa
    19741 aatatgccgc cctaaagggc ggcattcggg cttacttgct gctctgaagg ttgttaacca
    19801 ccagtaaatt gtcgccggag agactccggt tattatttac ttgggaaaac agcgcaccag
    19861 aaacaataag tacgatacag aagcaccgta tcttcattgt gcggtccatg cgaagaccgc
    19921 atcacgacag caccactggc gatattgccg cgatagcaag cgctgagacg cgcttgctct
    19981 aacggtcgcg gaacatcctt tccttaagcc agcgcccgct tgcgagacgg cggaaagggc
    20041 cgcagtggat aatacactaa cagaacaata ataactctct tttgcttgac aaaaagagag
    20101 ttactggcga gtattgtctc cgctgagacg cgcttgcatt actacggtaa tacaacctgc
    20161 tggaaaggcc gcgaaccgga ccagcaacaa ataaccgcct gattttggcg gttttttgtt
    20221 ttttaaagga aagtaaattt acatgtttaa gtaatatctt ttaccggtta gaatttttaa
    20281 ttttgagcag gatttgttta tatttgtctg aattcggact aataagccag gttattttat
    20341 taccgaacac ggcggaaagg gccggtgtcg ataatacgct aaataaacaa taattactct
    20401 cttttgcttg acaaaaagag agttactggt gagtattgtt ttgctgtgaa gcacctgcgt
    20461 tgctcatgta acacaagtca acctgctgga aaggccgcga accagaccag caataaaaaa
    20521 acgccaaatt cggcggtttt ttattgcctg taatatactt atttcactgg ctcaaccggt
    20581 aaatctgccc gcgcgcccca ttcactccat gcgccgtcgt acagtttcac gtttggcaca
    20641 tccagcgtcg cgagtgctaa caaaaccacg gctgccgtta caccagagcc gcagctgacg
    20701 ataatcggtt tgtcatagct gacgccgcga ccaaaaaata tcgcatccag ttcatcggtc
    20761 gtttttagtt cgccttcgcg caccagttcc gtccacggaa cgttcaatgc accgggaata
    20821 tgtccgcgac gtaaacctgg gcgaggttca tcaacttctg cgttaaaacg tgcagccggg
    20881 cgggcatcaa taatttgcgc cgtattttca tggcttgcca ataacacatc ggttactttc
    20941 accactgctt caggattaaa cgcggcgtta aactctcctt ccggcagctc tactgcacct
    21001 tcttctaata gcaggtcatc gcgctgccag cctgcaag
//
LOCUS       DADLTY010000069        20873 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.069, whole genome shotgun sequence.
ACCESSION   DADLTY010000069 DADLTY010000000
VERSION     DADLTY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20873
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.069"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="JN334_002087"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="JN334_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAY7438229.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="JN334_002088"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="JN334_002088"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAY7438230.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="JN334_002089"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="JN334_002089"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAY7438231.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="JN334_002090"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="JN334_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAY7438232.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="JN334_002091"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="JN334_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAY7438233.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /locus_tag="JN334_002092"
     CDS             complement(3426..4094)
                     /locus_tag="JN334_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAY7438234.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="JN334_002093"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="JN334_002093"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAY7438235.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="JN334_002094"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="JN334_002094"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAY7438236.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="JN334_002095"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="JN334_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HAY7438237.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="JN334_002096"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="JN334_002096"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAY7438238.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="JN334_002097"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="JN334_002097"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAY7438239.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="JN334_002098"
     CDS             8212..9075
                     /locus_tag="JN334_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAY7438240.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="JN334_002099"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="JN334_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HAY7438241.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="JN334_002100"
     CDS             10411..11028
                     /locus_tag="JN334_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAY7438242.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="JN334_002101"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="JN334_002101"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HAY7438243.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="JN334_002102"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="JN334_002102"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAY7438244.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="JN334_002103"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="JN334_002103"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAY7438245.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="JN334_002104"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="JN334_002104"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAY7438246.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="JN334_002105"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="JN334_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HAY7438247.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="JN334_002106"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="JN334_002106"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAY7438248.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="JN334_002107"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="JN334_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HAY7438249.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..>20873
                     /gene="xanP"
                     /locus_tag="JN334_002108"
     CDS             20347..>20873
                     /gene="xanP"
                     /locus_tag="JN334_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAY7438250.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQV"
BASE COUNT         5092 a         5665 c         5273 g         4843 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gtgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggt
//
LOCUS       DADLTY010000070        20779 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.070, whole genome shotgun sequence.
ACCESSION   DADLTY010000070 DADLTY010000000
VERSION     DADLTY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.070"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            49..579
                     /locus_tag="JN334_002109"
     CDS             49..579
                     /locus_tag="JN334_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7438251.1"
                     /translation="MSITGVNIGRLFREGFSNYDPIGVLNAMASQRAKEARGGELQIN
                     ELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFT
                     EVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAV
                     QEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="JN334_002110"
     CDS             597..1235
                     /locus_tag="JN334_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7438252.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="JN334_002111"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="JN334_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HAY7438253.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="JN334_002112"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="JN334_002112"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HAY7438254.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="JN334_002113"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="JN334_002113"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HAY7438255.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="JN334_002114"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="JN334_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HAY7438256.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="JN334_002115"
     CDS             complement(5552..6613)
                     /locus_tag="JN334_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HAY7438257.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="JN334_002116"
     CDS             complement(6610..8007)
                     /locus_tag="JN334_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HAY7438258.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="JN334_002117"
     CDS             8163..9161
                     /locus_tag="JN334_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY7438259.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="JN334_002118"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="JN334_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="JN334_002119"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="JN334_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="JN334_002120"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="JN334_002120"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAY7438260.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="JN334_002121"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="JN334_002121"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HAY7438261.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..15286)
                     /locus_tag="JN334_002122"
                     /pseudo
     CDS             complement(14232..15286)
                     /locus_tag="JN334_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(15296..16071)
                     /locus_tag="JN334_002123"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="JN334_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16089..17141)
                     /locus_tag="JN334_002124"
     CDS             complement(16089..17141)
                     /locus_tag="JN334_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAY7438262.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="JN334_002125"
     CDS             complement(17172..18014)
                     /locus_tag="JN334_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY7438263.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="JN334_002126"
     CDS             complement(18001..18882)
                     /locus_tag="JN334_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY7438264.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="JN334_002127"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="JN334_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20200..>20779)
                     /locus_tag="JN334_002128"
     CDS             complement(20200..>20779)
                     /locus_tag="JN334_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAY7438265.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5070 a         5668 c         5053 g         4988 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attatttttc cagtttact
//
LOCUS       DADLTY010000071        20691 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.071, whole genome shotgun sequence.
ACCESSION   DADLTY010000071 DADLTY010000000
VERSION     DADLTY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.071"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            81..350
                     /locus_tag="JN334_002129"
     CDS             81..350
                     /locus_tag="JN334_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HAY7438266.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="JN334_002130"
     CDS             344..709
                     /locus_tag="JN334_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAY7438267.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="JN334_002131"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="JN334_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HAY7438268.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="JN334_002132"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="JN334_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HAY7438269.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="JN334_002133"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="JN334_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HAY7438270.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="JN334_002134"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="JN334_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY7438271.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="JN334_002135"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="JN334_002135"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAY7438272.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="JN334_002136"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="JN334_002136"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HAY7438273.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="JN334_002137"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="JN334_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="JN334_002138"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="JN334_002138"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HAY7438274.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /locus_tag="JN334_002139"
     CDS             complement(13442..14668)
                     /locus_tag="JN334_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAY7438275.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14849..16447
                     /locus_tag="JN334_002140"
     CDS             14849..16447
                     /locus_tag="JN334_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /protein_id="HAY7438276.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16429..17187)
                     /locus_tag="JN334_002141"
     CDS             complement(16429..17187)
                     /locus_tag="JN334_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HAY7438277.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="JN334_002142"
     CDS             complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="JN334_002142"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HAY7438278.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="JN334_002143"
     CDS             complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="JN334_002143"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HAY7438279.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18595..20100
                     /gene="glpD"
                     /locus_tag="JN334_002144"
     CDS             18595..20100
                     /gene="glpD"
                     /locus_tag="JN334_002144"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAY7438280.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20369..20599)
                     /locus_tag="JN334_002145"
                     /pseudo
     CDS             complement(20369..20599)
                     /locus_tag="JN334_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4890 a         5692 c         5216 g         4893 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cacttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagcta ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttatt gtttctaaaa
    14761 agattatatc tttatatccg taaacggaga tttcccgcaa agccgattta ccgataatga
    14821 aatatcgcct tttataagga tatctaagat gcgtaaaaca gtggcttttg gctttgtcgg
    14881 taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcaaaatggc gtccgtcact
    14941 ctgtatatac cagcaagaat cgttggtcat cgatcgactg gaattattgc acgatgcccg
    15001 ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc cagaaacaga
    15061 agtggtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg tttacgcctg
    15121 cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact atttaattca
    15181 catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg aagcgcgtta
    15241 tctacccgcc cggttgatac aatcttcaac tccgcgcaaa aaagaacagc cgcgcggccc
    15301 aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg ccagccgctt
    15361 tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc ggtattgcca cgcgtaaccc
    15421 ccacttcaac cgcatgattg agcagatcga aaaagtggcg attaaatccc gcgcgccgat
    15481 tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg gcgcgacgca tcttcgagtt
    15541 aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg ccaccctgcg
    15601 cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa ggcgcgttta ccggggcgcg
    15661 ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc ttgatgagat
    15721 tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gctattgaag agaaaacctt
    15781 ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg ccggaacggt
    15841 gcgcgatttg cgccagctgg ttgccgaagg caaattccgc gaagatctgt acgcgcggat
    15901 caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata ttgaaccgaa
    15961 cctggattat gaagtggagc gccacgccac actcactggt gacagcgtgc gttttaacac
    16021 cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc caggcaacat ggcgcggtaa
    16081 ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta gcggacgcat
    16141 cactctggac gtggtggaag atgagataaa ccgtctgcgc tataactggc aggagagccg
    16201 cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc tcttcgaccg
    16261 tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt ctgctgccgg
    16321 acgacagctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg cggatcggct
    16381 acgcaaatac ctggcgcgtt ttggtctgac gtgggaagcc gtgcaggatc agcacagctc
    16441 cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg gggcgtcggt
    16501 gtagacggca tcaaccatgc tgatgctccc catattgacc atcgcattac ggccaaattt
    16561 tgaatggtca accaccagca taacgtggcg cgagttctca ataatggcgc gcttggtacg
    16621 aacttcgtgg taatcgaact ccagcagcga gccgtcgctg tcgatgccgc tgatccccag
    16681 aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc caatgatccc
    16741 gccatcgcgg ctacgtaatt cgccaccggc gagaataatg cggaaatctt cttttaccat
    16801 caacgtgtta gcaacgttga gattgttggt gacaatgcgc aaattgctgt gattgagcag
    16861 cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg agccattggg
    16921 gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg tggctttacg
    16981 atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcgccgccat gatggcgcag
    17041 gatcaggttt tgctctgcca gctcgttgag gtcgcggcga atagtctgcg ggctgacgga
    17101 gaagtgctcc accagttcct cggtactgac ataaccctgc tgtttaacca gttcgataat
    17161 accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt cgttttcgtg
    17221 cattgagcga atcaacaaaa gccatcgcta aacccacggc taatccggcg atgtgtgctc
    17281 cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag atcagcgcaa
    17341 agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg ccacgtagcc
    17401 agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac ggcccgctga
    17461 atttttgctg catatagccg cttaacaggg cgctgataag cgtaatgaca attagcttac
    17521 cgctaccgag gcgtttttcc accgcgccgc cgagatacca ccaccaaagc aggttaaaga
    17581 ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag aactcaaatt
    17641 tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg ccgagaattt
    17701 gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc ggacctgcgc
    17761 gttcacgcaa ggcagcaaag aaaggataac ggcgataatg caggccactg ccggtatggc
    17821 ctgccagcca gctggccgcc agataacgcg gatctgccgg gttttcgaga aaacgcgcca
    17881 gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg ctttggttat
    17941 gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac gcctgcgcca
    18001 cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat tccacacaaa
    18061 aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact gtcgctgcca
    18121 ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca gcagatactg
    18181 cgccgcccct ttgctgctat tgccgtgata acacatcacc atcaccggag tgtcaaagtc
    18241 gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct gcaccgcatg
    18301 ccccatagcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt tttcctgcaa
    18361 cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc tctctttctt
    18421 tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat aaacgccata
    18481 atgttataca tatcactcta aaatgttttt tcaatgttac ccaaagcgcg attctttgct
    18541 aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg cagcatggaa
    18601 accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc ggcagacgcc
    18661 gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg cgcgacctct
    18721 tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta tgaattccgc
    18781 ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc gcatatcgcc
    18841 ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg gatgattcgc
    18901 attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg atcaactggt
    18961 ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt cgaatattct
    19021 gactgttggg tagacgacgc ccgtctggta ctcgccaacg cccagatggt ggtgcgtaaa
    19081 ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa cggcctgtgg
    19141 attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc gcgcggcctg
    19201 gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca tctgccttcg
    19261 ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt gcatacccaa
    19321 aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat cccgtggatg
    19381 gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggtgatcc gaaagcagtg
    19441 aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca ctttaaaaag
    19501 cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct gtgtgatgat
    19561 gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca tgatgaaaac
    19621 ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg aaaactggca
    19681 gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc gtggacgaaa
    19741 gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc cgctcgcctg
    19801 cgccgccgtt acccgttcct gactgaatcg ctggcacgtc actacacgcg cacttacggc
    19861 agcaacagcg agctgctgct cggcaatgcg ggaacggtaa gcgatctcgg ggaagatttc
    19921 ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg ggtccgccgc
    19981 accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc ggatcaacaa
    20041 tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct ggcgtcgtaa
    20101 attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga caaacacaaa
    20161 actgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatat tgaatttgca
    20221 tggtcttgta ggccagataa gacgttcacg tcgcatccgg catgaacaaa gcgcactttg
    20281 tcaaaaatct aacctacttt ttaattcagg aaattaccgc aaagcccacg ccatcatacc
    20341 caacgtaaca aaactcaggc acgttcccca ctcgccccga gaaaaatagc attaatgcgc
    20401 ccagcgtcag cataaaaata ttgagcagaa ggtaaattgt tggcgtaaag agtaaattgc
    20461 aaacctcagg aatagcggca agcccagccc ccagcatggc aataaccgct gcaaaaaatt
    20521 gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga ggtcaaaatc cattttctat
    20581 tttgcattat tctttccatt ctttctgaat ggcgaaatta tactccccga gtccccttgc
    20641 cccttctgga cacttttccg aaatgatggc gaaaaaaacg ggaccctttg g
//
LOCUS       DADLTY010000072        20521 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.072, whole genome shotgun sequence.
ACCESSION   DADLTY010000072 DADLTY010000000
VERSION     DADLTY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.072"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="JN334_002146"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="JN334_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAY7438281.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="JN334_002147"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="JN334_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HAY7438282.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="JN334_002148"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="JN334_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAY7438283.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JN334_002149"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JN334_002149"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HAY7438284.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JN334_002150"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JN334_002150"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HAY7438285.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="JN334_002151"
     CDS             4449..4814
                     /locus_tag="JN334_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HAY7438286.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="JN334_002152"
     CDS             4964..5482
                     /locus_tag="JN334_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HAY7438287.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="JN334_002153"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="JN334_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="JN334_002154"
     CDS             6713..7195
                     /locus_tag="JN334_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAY7438288.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JN334_002155"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JN334_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAY7438289.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JN334_002156"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JN334_002156"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAY7438290.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JN334_002157"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JN334_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAY7438291.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JN334_002158"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JN334_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAY7438292.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JN334_002159"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JN334_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAY7438293.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10864..11730
                     /locus_tag="JN334_002160"
     CDS             10864..11730
                     /locus_tag="JN334_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HAY7438294.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLVIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLMHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="JN334_002161"
     CDS             11751..13037
                     /locus_tag="JN334_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY7438295.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JN334_002162"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JN334_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAY7438296.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="JN334_002163"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="JN334_002163"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAY7438297.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="JN334_002164"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="JN334_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAY7438298.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="JN334_002165"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="JN334_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAY7438299.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="JN334_002166"
     CDS             complement(16985..17275)
                     /locus_tag="JN334_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAY7438300.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17741)
                     /gene="ratA"
                     /locus_tag="JN334_002167"
     CDS             complement(17265..17741)
                     /gene="ratA"
                     /locus_tag="JN334_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HAY7438301.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGG
                     WKFTPLSQEACRIEFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="JN334_002168"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="JN334_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAY7438302.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="JN334_002169"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="JN334_002169"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="JN334_002170"
     CDS             19137..20378
                     /locus_tag="JN334_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7438303.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5301 a         5356 c         4815 g         5049 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatttgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acatcccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagaatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attgcaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccaa gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgttgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtatttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DADLTY010000073        20487 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.073, whole genome shotgun sequence.
ACCESSION   DADLTY010000073 DADLTY010000000
VERSION     DADLTY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.073"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..209)
                     /locus_tag="JN334_002171"
     CDS             complement(<1..209)
                     /locus_tag="JN334_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7438304.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JN334_002172"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JN334_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAY7438305.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JN334_002173"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JN334_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAY7438306.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JN334_002174"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JN334_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAY7438307.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JN334_002175"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JN334_002175"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HAY7438308.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JN334_002176"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JN334_002176"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HAY7438309.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JN334_002177"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JN334_002177"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HAY7438310.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="JN334_002178"
     CDS             complement(4598..5320)
                     /locus_tag="JN334_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7438311.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="JN334_002179"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="JN334_002179"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7438312.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="JN334_002180"
     CDS             6520..6738
                     /locus_tag="JN334_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HAY7438313.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="JN334_002181"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="JN334_002181"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY7438314.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="JN334_002182"
     CDS             complement(7472..7672)
                     /locus_tag="JN334_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HAY7438315.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9370)
                     /gene="kefB"
                     /locus_tag="JN334_002183"
     CDS             complement(7682..9370)
                     /gene="kefB"
                     /locus_tag="JN334_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HAY7438316.1"
                     /translation="MGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFSELGVVFLMFI
                     IGLELNPSKLWQLRRSIFGVGAAQVLLSAALLAGLLMLTDFAWQAAVVGGIGFAMSST
                     AMALQLMREKGMNRSESGQLGFSVLLFQDLAVIPALALVPLLAGSADEHFDWMKIGMK
                     VLEFVGMLIGGRYLLRPVFRFIAASGVREVFTAATLLLVLGSALFMDALGLSMALGTF
                     IAGVLLAESEYRHELETAIDPFKGLLLGLFFISVGMSLNLGVLYTHLLWVVISVVVLV
                     AVKILVLYLLARLYGVRSSERMQFAGVLSQGGEFAFVLFSTASSQRLFQGDQMAPLLM
                     KLVDKWLSRQFNGPEEEDEKPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLER
                     DISAVNLMRKYGYKVYYGDATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHF
                     PHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLH
                     FRRLDMRMLRELIPMHADTVQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="JN334_002184"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="JN334_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HAY7438317.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="JN334_002185"
     CDS             10101..12014
                     /locus_tag="JN334_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7438318.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="JN334_002186"
     CDS             12014..13036
                     /locus_tag="JN334_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7438319.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="JN334_002187"
     CDS             13030..13248
                     /locus_tag="JN334_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HAY7438320.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="JN334_002188"
     CDS             13302..14171
                     /locus_tag="JN334_002188"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HAY7438321.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="JN334_002189"
     CDS             complement(14226..14630)
                     /locus_tag="JN334_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAY7438322.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="JN334_002190"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="JN334_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HAY7438323.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="JN334_002191"
     CDS             15615..17705
                     /locus_tag="JN334_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438324.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="JN334_002192"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="JN334_002192"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HAY7438325.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="JN334_002193"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="JN334_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HAY7438326.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="JN334_002194"
     CDS             complement(19673..20275)
                     /locus_tag="JN334_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HAY7438327.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="JN334_002195"
     CDS             complement(20265..20432)
                     /locus_tag="JN334_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HAY7438328.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
BASE COUNT         5035 a         5590 c         5062 g         4800 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggt
//
LOCUS       DADLTY010000074        20287 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.074, whole genome shotgun sequence.
ACCESSION   DADLTY010000074 DADLTY010000000
VERSION     DADLTY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20287
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.074"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(61..819)
                     /gene="aroD"
                     /locus_tag="JN334_002196"
     CDS             complement(61..819)
                     /gene="aroD"
                     /locus_tag="JN334_002196"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAY7438329.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="JN334_002197"
     CDS             complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="JN334_002197"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HAY7438330.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1728..2942)
                     /locus_tag="JN334_002198"
     CDS             complement(1728..2942)
                     /locus_tag="JN334_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7438331.1"
                     /translation="MYFSYFLHGISVITLAQNMTSLSEKFSTDNAGIAYLISGIGLGR
                     LISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLTLAYGLAVCVGIANSALD
                     TGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYMLLNNIWYGYGLIIPGILF
                     VLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSSVLFGVAAFSTFYVIVVW
                     MPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKMVRPIWANVFNSALATIT
                     AAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFPKSKAKVTSIYMMMGGLA
                     NFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRYYRVFIIPENDVRFGERK
                     FSTRLNTIKHRG"
     gene            complement(3182..4387)
                     /locus_tag="JN334_002199"
     CDS             complement(3182..4387)
                     /locus_tag="JN334_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7438332.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4487..4843)
                     /locus_tag="JN334_002200"
     CDS             complement(4487..4843)
                     /locus_tag="JN334_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HAY7438333.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4979..5086)
                     /gene="rprA"
                     /locus_tag="JN334_002201"
     ncRNA           complement(4979..5086)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="JN334_002201"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="JN334_002202"
     CDS             complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="JN334_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HAY7438334.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6773..9829
                     /locus_tag="JN334_002203"
     CDS             6773..9829
                     /locus_tag="JN334_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY7438335.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9826..10236
                     /gene="menI"
                     /locus_tag="JN334_002204"
     CDS             9826..10236
                     /gene="menI"
                     /locus_tag="JN334_002204"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HAY7438336.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10336..10524
                     /locus_tag="JN334_002205"
     CDS             10336..10524
                     /locus_tag="JN334_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438337.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11072..11440
                     /gene="sufA"
                     /locus_tag="JN334_002206"
     CDS             11072..11440
                     /gene="sufA"
                     /locus_tag="JN334_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HAY7438338.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11449..12936
                     /gene="sufB"
                     /locus_tag="JN334_002207"
     CDS             11449..12936
                     /gene="sufB"
                     /locus_tag="JN334_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAY7438339.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12946..13692
                     /gene="sufC"
                     /locus_tag="JN334_002208"
     CDS             12946..13692
                     /gene="sufC"
                     /locus_tag="JN334_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAY7438340.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13667..14938
                     /gene="sufD"
                     /locus_tag="JN334_002209"
     CDS             13667..14938
                     /gene="sufD"
                     /locus_tag="JN334_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAY7438341.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14935..16155
                     /gene="sufS"
                     /locus_tag="JN334_002210"
     CDS             14935..16155
                     /gene="sufS"
                     /locus_tag="JN334_002210"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HAY7438342.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16168..16584
                     /gene="sufE"
                     /locus_tag="JN334_002211"
     CDS             16168..16584
                     /gene="sufE"
                     /locus_tag="JN334_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HAY7438343.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16733..17737
                     /gene="ldtE"
                     /locus_tag="JN334_002212"
     CDS             16733..17737
                     /gene="ldtE"
                     /locus_tag="JN334_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HAY7438344.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17800..18036)
                     /locus_tag="JN334_002213"
     CDS             complement(17800..18036)
                     /locus_tag="JN334_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HAY7438345.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18347..19759)
                     /gene="pykF"
                     /locus_tag="JN334_002214"
     CDS             complement(18347..19759)
                     /gene="pykF"
                     /locus_tag="JN334_002214"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HAY7438346.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5316 a         4861 c         5177 g         4933 t
ORIGIN      
        1 cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc
       61 ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg
      121 cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag
      181 acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg
      241 ctcctgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat
      301 cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat
      361 ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg
      421 cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc
      481 gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt
      541 ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc
      601 tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc
      661 ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc
      721 ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc
      781 cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg
      841 gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt
      901 gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat
      961 cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac
     1021 gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt
     1081 ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc
     1141 ttcagcaaag gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc
     1201 gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag
     1261 tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc
     1321 ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg
     1381 aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt
     1441 gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag
     1501 ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat
     1561 tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc
     1621 tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag gataagccat
     1681 caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc
     1741 ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa accgcacatc attctcagga
     1801 ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc
     1861 gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta
     1921 atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg
     1981 gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca
     2041 gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg
     2101 tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc
     2161 cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg
     2221 taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat
     2281 ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc
     2341 gatgaaacac cttccagcca gactaaaggt ttgctgttca tttgcggtaa ttcattagct
     2401 acgctggcgt ccaccaactg gctggggaat ttgcttttca acagcatcag cgtgatcagt
     2461 acaaacagaa tacccggaat aatcagccca tagccgtacc agatattatt gagcaacata
     2521 tagctcacca gcattgggta gaacatttgc ccaaatgaca ccatcgcttt aaccagtatg
     2581 accgccgaac cagaggcttt cggaaagcat tccatgagcg cggggtagcc acccgtatcc
     2641 agcgctgagt tagcgatacc tacgcacact gccagaccgt aggcgagagt taaattcggg
     2701 caagcgggaa taccaaagaa gaatagcaga tacattatta ctgccattaa tatcaccgcc
     2761 cgacgaccaa acttatcgga gatcacaccg aagaataaaa tactgatcaa tcgccccaaa
     2821 ccgataccgg aaattaaata ggcaatgccc gcgttgtcag tggaaaactt ttctgacaga
     2881 gatgtcatat tttgggcaag cgtaataaca ctaatgccgt gcaggaaata gctgaagtaa
     2941 atacaaagaa cagccaggat aaatggcgtg ctgaaagata atttcgttac cgtacgtatt
     3001 gttctccgac ggtttacata attactatac tgaagatgat tttccctggg tacatggcat
     3061 aaagtttatg tttccaggaa tattgcatgt catagtttgt ttttgatcgc gattttcctt
     3121 agttagcgca gaaagagtta tttatccgta agatcttgca tgacctgaaa agaatattgc
     3181 attacccacc cggagcgaca ttttccttta gcgagtgatg ccgcgttttt ttgcggctgc
     3241 gtaagccgat aaacagtgcc agtaaaaaac cgatggcagt atcgaaccac ataatatcgg
     3301 caatacttct ttgggacaga tgagccgtaa tcaacggaat agtaaaggtc gcaatactgc
     3361 ccgtactgta atagatccct gtagctttac ctttagcgta agggaaacgt tcagccatta
     3421 acgtcaggcc aatttgcaca acacctccag cagaggtaaa accaatgaca aaagcaaata
     3481 ttatcaccac ataaaatgtg ggatgcaggc agacggtaaa cagagcaata aatgagataa
     3541 aggtgtacag catcagtaat gttgttgggc gaacggtatt acgaatgagt ggagcggtaa
     3601 taaatacaca aagcagcgaa cccacggtgt agatactgag tagtttgatc gacatagtgt
     3661 atgacatgcc tgcaacaaat tgtccgtact gtgccagcca ctggctaacc agataaaatg
     3721 ttgccattga gatatagcca tataaggtat aactggctaa atcgataatt gaacagcgat
     3781 gttccgtaga gctggtggtt ttctttatga caggtaagcg acgacccgga tggggtggga
     3841 acgtacaacg gtataaaaac agagcgttaa taaacataat gcctgcagca atcataaagg
     3901 accaaccgaa ccacagttca gcccacacta acaggctaat gattagcggt aataaaaatt
     3961 gtccgctgga aacaaatgct ttaattaaaa tattggctgt cccaggtgag cgtggaaaag
     4021 cttccatcaa actgggataa gtgcctgcat cgagaaaact gtttgccatt cccgccagaa
     4081 agccaaaaac ataagcgata atgatgttat tggtctgcag gatgtcaaaa aagaaggcca
     4141 tatagcagca catcccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc
     4201 ctgcaaaaag caagacactt aatcgaccaa tgcccagcga tgagataact atcgagacac
     4261 ccgcggcatt agtctgccaa agtgtctcca gcgaggccat attcaggctc atcaaaatga
     4321 cgcccatacc atgcaccagg taattaaaat acaacccaag tgcggtaggg taatagggat
     4381 ttttcatagg ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat
     4441 ccattcatta ttgttgtaac aacgacaatt cctttctgtg gaaaaattaa caaagccgct
     4501 ccagatcgtg tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat
     4561 tgccatcagg gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt
     4621 tgccgatgcg gttatttatt ttctctatga tggcatgaag atgttttttt cgttgctgac
     4681 gcatagccaa cagttgctcg actacacaat caggaatggc gcatttgcca ttttcccact
     4741 gacgccagct ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca
     4801 tggcaaaaat atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg
     4861 aaataaacaa taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca
     4921 gtatcttgtg caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt
     4981 aaaaaaaagc ccatcgtggg agatgggaaa agactacaca cagcaattcg ttgtttcact
     5041 caggggattt ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg
     5101 catacggact ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt
     5161 gcgacaggta ctaccagctg aatggcggta agcaggaaaa tatggggaag agcaagcaaa
     5221 tatcaattag cggtgattaa caatcaggcg gcagagccgc ctgatgagaa attacttatt
     5281 cggtttctcg atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggtggcgg
     5341 gacttcttcc acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat
     5401 aaacagaccg atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat
     5461 cagcggtaaa tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc
     5521 aaccacaccg ctccacacta acaataccgt tccccaggtg gtatcgccgg tccagtagag
     5581 ccagataatc gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggagaggat
     5641 cattaacacc gttagcaacg ttgcataagg tacgccggat acggcgaggc cgataccgcc
     5701 aagcactgcc tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg
     5761 tgccgccagc agcacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg
     5821 aatgccttgt gccacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag
     5881 cgcacaatgc accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt
     5941 ggtgccaata taagggcgga ctttcgccat gatcgccgta ccccccatat ccagcaagtt
     6001 gtgccagcct gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc
     6061 gggtaacgtc atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat
     6121 actgttaacc agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac
     6181 cagaacggcg agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca
     6241 ggtggcgata accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca
     6301 cagacatgcc acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc
     6361 cctgggctga cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg
     6421 acgcgacgtg aacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa
     6481 caggccgatt ctgcccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact
     6541 caggcaacac gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt
     6601 cgttcacccc agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct
     6661 gcaactcgaa atccatagag taaataaaca tcacctttca gtgcgctatt gcagccgcct
     6721 gaaagaatag tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc
     6781 acagatttcc ctggcacccg gcgtcgttca actggtgctt aattttttgc aagagctgga
     6841 gcaacaaggt tttaccggcg atgcggcgac aagttatgcc gatcgtctga caatgtcgac
     6901 cgacaacagt atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga
     6961 tgtggcgctg atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc
     7021 ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat
     7081 gtcccgccat atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga
     7141 ggccggggtg ataaaagacc aactcaatca gtacctgaaa ccgttcggct acttttttgc
     7201 gccggaactt tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc
     7261 cggtcaggga tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt
     7321 gttgttgggg ggcgatattc tcgatacgca acctttaccc gtcgagctgg cggaaacgct
     7381 gggtaaatcc aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca
     7441 gcaacgccag ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga
     7501 tctgcgtcat gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg
     7561 ttcagaaggg acgctggcct ttattaccga agcgcggctg gatattacgc gcttgcctaa
     7621 agtgcgccgt ctggtgaatg tcaaatatga ctcttttgac tctgcgctgc gtaacgcgcc
     7681 gtttatggtt gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct
     7741 ggcgcgggaa gatattgtct ggcattccgt cagcgagttg attaccgata tgcctgacaa
     7801 agagatgctc ggactgaaca ttgtggaatt tgctggtgat gatgaggcgc tgattgacga
     7861 gcgagttaat gcactctgtg tgcggcttga tgagctgatt gccagccaac aggcaggtgt
     7921 gattggctgg caggtgtgcc gcgagctggc gggggttgaa cgtatctatg cgatgcgcaa
     7981 aaaagccgtt ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga
     8041 ggatacctgc gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct
     8101 cgacagccac ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt
     8161 ccgtccggca ctggatatgt gcgatccgca acaagagatt ttgatgaagc aaatctctga
     8221 tgacgtggtg gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg
     8281 ttttcgcgct gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa
     8341 agtgaaagcg gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga
     8401 aggtctcgat gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca
     8461 gatccccatt gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt
     8521 atgcttcaac tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg
     8581 gattcattca ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgct tgttggcgga
     8641 tcgcggcgtt gacccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg
     8701 gacgttaatt gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc
     8761 acacgaagtc aaagaggcga tgtcgggctg tctggcctgt aaagcttgtt cgacccagtg
     8821 ccccatcaaa attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg
     8881 ttatttacgc ccgcttcgcg accacctcgt tgctacggtc gagagctacg caccgctgat
     8941 ggcacgcgcg ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc
     9001 ggaaaaacat atcggcatgg ttgatttgcc actgctgtcg gtcccctcgc tacaacaaca
     9061 aatggtgggg catcgctcgg caaacatgac gctggaacag cttgaagctc tcaatgcaga
     9121 gcagaaagcg cgcacggtgt tggtggtgca ggaccccttt accagctatt acgatgcgca
     9181 agtggtggcg gattttgtcc gtctggttga aaaattaggt ttccagcctg tgttactgcc
     9241 attttcgcca aatggcaaag cccagcatat caaaggcttt cttaatcgtt ttgcgaagac
     9301 ggcgaaaaag acggcggatt tcctcaaccg tatggctaag cttgggatgc caatggtggg
     9361 cgtcgatccg gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga
     9421 acgtggcgag tttaacgtct tacttgcgaa tgaatggctg gcaagcgcac ttgagtcaca
     9481 tccagtggct acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaggttac
     9541 cgctttgccg ggcgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact
     9601 ggaaaatgtc agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa
     9661 ccatgaaaat tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc
     9721 gcgtaaccgc tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggttgaagg
     9781 cacgggggta cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacgg
     9841 aaaatcaccc tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttcctggat
     9901 attcgctttg aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg
     9961 acaaagcagc ctttcgggtt gctgcatgga ggagcatccg tggtactggc cgaaagtatc
    10021 ggttccgttg ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg tctggaaatc
    10081 aatgctaatc acgtccgctc ggcacgagaa gggcgggtgc gcggcatatg taaaccgttg
    10141 catctcggtt cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg
    10201 tgctgttcgt cacgattgac gaccgccatt ttgtgacgtt gtcaccagaa aagtgtgacg
    10261 catattgctt ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct
    10321 aaaagcgagg atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta
    10381 cgccgtgacc agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa
    10441 cttgaatttg ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttc
    10501 gcctggcgtc tgggcgtgca ttgatcctga cttaatgccg caagttcgcc aaatcgacct
    10561 ggattaacct ccgtccaggt tcgatgacct tcttaatact gcttatctac cgcctgatat
    10621 tgttcaaaaa taatactaat cgcagtttgt gttaaaacgg cgggttagct ttatgagatt
    10681 attcttatcg ccccttcaag agctaagcca ctgagagtgc cggagataag cgccggatgg
    10741 ggtagaaacc cttaagcctg tgtcgcacag acttaagggt tttcttattt ctgaacgctg
    10801 cactttttta cctatctctt aaatagtcat tttcaataca actttttctt tttcatcaca
    10861 gccttaatgc tccttagata ataaccatta tctaacaatg agatacctaa ttcttagcgt
    10921 gcctgttaac ccacacatca ggggctatgc ttattaaaag caggcaaaaa gaatgtttta
    10981 cggtaaagcc cctgcgtttg ctgggttgaa ctgataatca ttatcactaa catgctgtta
    11041 tacgctgaaa gcgatgaagt gaggtaaata gatggacatg cattcaggaa cctttaaccc
    11101 acaggatttc gcctggcaag gcttaacgct gacacccgca gcggcgatac acatccgcga
    11161 gctggtggca aagcagccag gtatggtcgg cgtgcgctta ggtgtgaagc aaacgggctg
    11221 cgcgggcttt ggctatgtgc tcgacagtgt tagcgagccg gacaaagacg atctgctgtt
    11281 tgaacacgac ggcgcgaagc tgtttgtccc gctgcaatcg atgccgttta ttgatggcac
    11341 ggaagtcgat ttcgttcgtg aaggacttaa tcagatattc aaatttcaca accctaaagc
    11401 ccagaatgaa tgtggctgtg gcgaaagctt tagggtatag gcggtactat gtctcgtaat
    11461 actgaagcaa ctgacgatgt caaaacctgg accggcggcc cgctgaacta taaagaagga
    11521 ttcttcaccc agttagccac cgatgagctg gcaaagggga taaacgaaga ggtggtgcgc
    11581 gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg agtttcgtct aaacgcctat
    11641 cgcgcatggc tggagatgga agaaccgcac tggttgaaag cgcactacga caagctgaat
    11701 tatcaggatt acagctacta ctcagcacca tcgtgcggta attgtgacga cacttgcgcg
    11761 tctgaacctg gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa agaggtggag
    11821 gcggcgtttg agcagttggg cgttcccgtg cgggaaggca aagaggtggc ggtggatgcc
    11881 attttcgact ctgtttcggt tgccaccact tatcgcgaaa aactggcgga gcagggaatt
    11941 attttctgtt cctttggtga ggcgatccac gatcacccgg aactggtgcg taaatatctc
    12001 ggcaccgtgg tgccggggaa tgacaacttc tttgcagcac ttaatgcggc ggtagcctct
    12061 gacggtacgt ttatttatgt gcctaaaggc gtgcgctgcc cgatggaact ttccacctat
    12121 tttcgcatta acgcggaaaa aaccgggcag tttgagcgca caattctggt ggccgacgaa
    12181 gacagctacg tcagctacat tgaaggctgt tccgctccgg tgcgtgacag ctatcagttg
    12241 cacgcggcag tggtggaagt catcatccat aaaaacgccg aggtgaaata ttccacggta
    12301 caaaactggt tccctggcga taacaacacc ggcggtattc tcaacttcgt caccaagcgt
    12361 gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac cgggtcagcg
    12421 attacgtgga aatatcccag ctgcattttg cgcggcgata actccattgg tgagttttac
    12481 tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg gcaccaagat gatccacatc
    12541 ggtaaaaaca ccaaatcgac cattatctcg aaagggatct ctgccggaca tagtcagaac
    12601 agttatcgcg gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa tttcactcag
    12661 tgcgactcaa tgctgattgg cgctaattgt ggggcgcata ccttcccgta tgtcgaatgt
    12721 cgcaataaca gcgcacaact ggagcacgag gcaacgacat cacgtattgg tgaagatcaa
    12781 ctgttttact gcctgcaacg cgggatcagc gaagaagacg ccatctcgat gattgttaac
    12841 ggtttctgca aagacgtgtt ctcagaactg ccgctggaat ttgccgttga agcacaaaaa
    12901 ctcctcgcca tcagtcttga acacagcgtc ggataaggaa taaacatgtt aagtattaaa
    12961 gatttacacg tcagcgtgga agataaagct atcctgcgtg gattaagcct cgacgttcgt
    13021 cccggcgaag ttcacgccat tatggggcca aacggttcgg gcaaaagtac cttatcggca
    13081 acgcttgccg ggcgcgaaga ttatgaagtg acgggcggca cggttgagtt caaaggcaaa
    13141 gatttgcttg cactgtcgcc ggaagatcgt gcaggcgaag gcatctttat ggccttccag
    13201 tatccggtgg agattccagg cgtcagtaac cagtttttcc tgcaaacggc acttaatgca
    13261 gtgcgcagct atcgcggcca ggaaacgctc gaccgctttg attttcagga tttgatggaa
    13321 gagaaaatcg ctctcctgaa gatgccggaa gatttattaa cccgttcggt aaacgttggt
    13381 ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct ggaaccggag
    13441 ttatgcattc ttgatgagtc ggactccggg ctggatattg acgcattaaa agtggtcgcc
    13501 gatggcgtga actcgctgcg tgatggcaag cgctcattca tcattgttac gcactaccaa
    13561 cgcattctcg actacatcaa gcctgattac gttcatgtgc tatatcaggg acgaattgtg
    13621 aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc agggttatgg ctggcttacc
    13681 gaacagcagt aatgcgctgc aacagtggca tcacttgttt gaagctgaag ggacaaaacg
    13741 ctcaccgcaa gcacagcagc atttacaaca attgctgcgt accggactgc cgacacgtaa
    13801 acatgaaaac tggaaatata cgccgctgga agggctgacc aatagtcagt ttgtcagcat
    13861 tgcgggagat atatccccac agcagcgtga tgccttagcg ttaacgttag acgccgtgcg
    13921 gctggtgttt gtcgatggac gttacgtgcc cgcgctgagc gatgcaattg aaggcagcgg
    13981 atatgaagtg agcattaacg acgaccgtca ggggttaccc gacgctattc aggcggaagt
    14041 gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg catatcgccg tgaagcgcgg
    14101 tcaacggccg gcaaagccat tgctgttaat gcatatcacc cagggcgtgg caggtgaaga
    14161 ggtgaacact gcccattacc ggcatcatct ggatctggcg gaaggtgccg aagcaacggt
    14221 gatcgaacat tttgtcagcc ttaatgatgc tcgtcatttt accggggcac ggttcactat
    14281 caatgtcgca gcgaacgccc acttgcagca tatcaagctg gcgtttgaaa acccggtcag
    14341 tcaccacttt gcccataacg atttgttgct ggctgacgat gccaccgcat ttagccatag
    14401 tttcctgctg ggtggcgcag tgttacgaca caacaccagt acgcaactca atggcgaaaa
    14461 cagcacgctg cggatcaata gcctggcgat gccggtgaaa aacgaggtgt gtgatacccg
    14521 tacctggctg gaacacaata aaggtttttg taacagccga cagttgcaca aaactatcgt
    14581 cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc acgccatcaa
    14641 aacggatggt cagatgacca acaataatct gctgatgggc aaactggcgg aagtggatac
    14701 aaaaccgcag ctggaaatct atgcagatga tgtgaaatgc agccacggcg cgacggtggg
    14761 gcgtattgat gatgaacaga tgttctatct gcgctcgcgc gggatcaatc agcaggatgc
    14821 ccagcagatg atcatttacg cctttgctgc cgaactgacg gaagcactgc gtgatgaggg
    14881 gcttaaacag caggtgctgg cccgaatcgg tcaacggctg ccaggaggtg caagatgact
    14941 ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt cgcgtgaggt aaacggtttg
    15001 ccgctggctt atctcgacag cgccgccagt gcacagaaac cgagccaggt gattgacgcc
    15061 gaggccgagt tttatcgtca tggctacgcg gcggtgcatc gcggtattca taccttaagc
    15121 gcccaggcga ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt tattaatgcc
    15181 cgttcggcgg aagagctggt gtttgtccgc ggcacgacgg aagggatcaa tctggtcgcc
    15241 aatagctggg gcaacagcaa tgtgcgggcg ggcgataaca tcatcatcag tcagatggag
    15301 caccacgcta acattgtccc ctggcagatg ctttgcgcac gcgttggcgc agagctgcgt
    15361 gtgatccctc tcaaccccga cggtacgctg caactggaga cgctgcctac gctgtttgat
    15421 gagaaaactc gcctgctggc aattactcat gtctccaacg tgcttggcac agaaaatcca
    15481 ctggcggaaa tgatcacgct tgcgcaccag catggcgcaa aagtgctggt ggatggcgct
    15541 caggcggtga tgcatcatcc ggtggatgtt caggcgctgg attgcgactt ttacgtgttc
    15601 tccgggcata aactgtatgg ccccaccgga attggcattc tttatgtgaa agaagccttg
    15661 ttgcaggaga tgccgccgtg ggaagggggc ggttctatga tcgccaccgt cagcctgagt
    15721 gaaggcacta cctggaccaa agcaccatgg cggtttgaag ccggtacacc caataccggg
    15781 ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg cgctggggct taatagcata
    15841 gccgagtatg aacagaatct gatgcattat gcgctatcac agctggaatc tgtaccggat
    15901 ctcactctct atggccaaca aaacaggctt ggcgttattg cttttaatct cggtaaacac
    15961 cacgcctatg atgttggcag ttttctcgat aattacggca ttgctgtgcg taccggacat
    16021 cactgcgcta tgccattaat ggcctattac aacgtccctg cgatgtgtcg ggcgtcgctg
    16081 gccatgtata acacccatga agaagtggag cgtctggtga ccggcctgca acgtattcac
    16141 cgtctgctgg gataacaggg aggcactatg gctttattgc cggataaaga aaaattgctg
    16201 cgtaattttt tacgctgcgc caactgggaa gagaaatatc tctacattat tgagctgggc
    16261 cagcgtctgc cagaattacg cgccgaagac ggaagcccac aaaatagcat tcagggctgc
    16321 cagagtcagg tgtggattgt catgcgccag aatgcgcagg gaattattga attgcagggc
    16381 gacagcgatg cggcgattgt gaaagggctt attgcggtcg tctttattct ctacgatcag
    16441 atgacgccgc aggatattgt caatttcgat gtgcgtccgt ggtttgaaaa aatggcgctc
    16501 acccaacatc tcaccccatc tcgttcacaa ggtctggaag cgatgattcg cgcaattcgc
    16561 gccaaagccg ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt
    16621 gccggatggt caatgtttac actgaccggc acaggagtgt tttggtttca ggtgaacata
    16681 agcaccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaacg
    16741 cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc
    16801 ggttgattat ccgctaccac caaccggaag ccgactggtt gggcaaaatc aaacgtatac
    16861 ggtgcaagaa ggggataaaa accttcaggc tattgcccga cgttttgata ctgcagcaat
    16921 gttgatcctt gaagccaata acactatcgc cccggtgcca aaacctggta cgacgataac
    16981 tattccttca caactgttat tacctgatgc accgcgtcag gggattatcg ttaaccttgc
    17041 agagctgcgc ctttattatt atccgccggg agaaaatatt gtgcaggttt atccaatagg
    17101 tattggattg caggggctgg aaacgccggt gatggaaacg cgtgttgggc agaaaatccc
    17161 taacccaacc tggacgccta cggcaggcat tcgtcagcgt tcgctggagc gtggcattaa
    17221 attaccgcca gtcgttcctg ccggaccaaa taacccgcta ggacgttacg cactgcgcct
    17281 cgcgcatggt aatggcgaat acctcattca tggcaccagt gcgccggaca gcgttggttt
    17341 gcgcgtcagt tcagggtgta ttcgcatgaa tgcaccggat attaaagcct tgttctccag
    17401 cgtgcggacg ggaacgccgg tgaaagtgat caacgaaccg gtgaaatatt ccgtggaacc
    17461 taacgggatg cgttatgttg aagtacatcg accactatca gcagaagaac agcaaaacgt
    17521 tcagacaatg ccatacacac tgccagcagg ctttacgcaa tttaaagaca ataaggctgt
    17581 agatcagaag ttagtcgata aagcgttgta tcgtcgggca gggtatccgg tttcggtgag
    17641 cagtggagca actcccgcag ccagcaatgc gccttcagta gagtcagcgc agaatggtga
    17701 accagagcaa gggaatatgt tacgcgcgac gcagtagtgg taaatagcag acaaaaaaat
    17761 ggcgcacaat gtgcgccatt tttcacttca caggtactat tacttgcggt atttagtagc
    17821 catgttgtcc agacgctggt tagcacgagc tgcgtcatct ttagcagcct gaacgtcgga
    17881 acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta gcgttcagag tctgaacgtc
    17941 agaagacagc tgatcgattt tagcgttgct ggagcaacct gccagcagag tagaacccag
    18001 gattaccgcg cccagtacca gtttagtagc tttcattatt aataccctct agattgagtt
    18061 aatctccatg tagcgttaca agtattacac aaagtttttt atgttgagaa tatttttttg
    18121 atgggaaggc acttattttt gatcgttcgc tcaaagaagc atcgaacgct gggttttaga
    18181 taaaaaaatt gagagaaaac aggatgcttc catcggattc atcttaaata aagtgagatt
    18241 atatagtgaa atccgatttg attttttatt gcttctggtt atcgattaaa taaaaaaagc
    18301 gcccatcagg gcgcttcgat atacaaatta attcacaaaa gcaatattac aggacgtgaa
    18361 cagatgcggt gttagtagtg ccgctcggta ccagtgcacc agaaaccata actacgacgt
    18421 cacctttgtg tgccagaccg ctctgcagag ccagttcttt acccagacgg tagaaatcat
    18481 cagtagaagt gatctcttta accagctgcg gcacaacgcc tttgctcagt accaactgat
    18541 gagccgtttt ttcgttggtg gtcagtgcca ggatggtggc atccgggaag tatttacgta
    18601 ctgcgcgagc agatttaccg ccctgggtag caaccacgat cagcggagca tccagttttt
    18661 cagcagtttc aacggcacca cggcataccg cttcggtgat gcgcagttta cggttgtcat
    18721 tgttgaactc gagacggctg ttcatcacgc ggtcggtacg ttcgcagatg gtcgccatga
    18781 tagaaaccgc ttccagcggg tatttacctt ttgcggattc accagacagc atcactgcgt
    18841 cagtaccgtc gaggatggcg tttgcaacgt caccggcttc tgcgcgagtc gggcgtgggt
    18901 ttttgatcat ggaatccagc atctgggtcg cagtgataac gactttacgt gcacggatac
    18961 atttttcgat catcatcttc tgggcgaaga taacttcttc taccgggatt tctacaccca
    19021 ggtcgccacg cgcaaccatg atgccgtcag aggcttcgag gatttcgtcg aagttgttga
    19081 ggccttcctg gttttcgatt ttggagatga tgtggatgtt ttcgccgccg tgcgctttca
    19141 ggtgctcacg gatttcgata acgtcagaac gcttacgaat aaaggaagca gcaacaaagt
    19201 ctacgccttt ttcgcaacca aagatcaggt cctgtttgtc tttttcagcc agtgctggca
    19261 gagcaatgga aacgccaggc aggttcacac ctttgttttc gcccaggtca ccgttgttca
    19321 gcactttaca gataactttg ttaccttcaa tggcggtaac ttccataccg atcagaccat
    19381 cgtcaaccag tacggtgttg ccaacagaca ggtcagtagt gaaaccttca tacgttaccg
    19441 caaccatttc gctgttgccg ataacagatt tatcagtggt gaaagtaaag gtctgaccag
    19501 ctttcagaga aacatcgtta ccgccttcca gtttcatggt gcggatttcc ggacctttgg
    19561 tatcaagcag gatagcggcg gttttaccag ttttgctcat cacgttgcgc agattctgaa
    19621 tgcgctgacc gtgttctgca tagtcaccat gagagaagtt cagacgcata acgttcatgc
    19681 cagcgtccag cattttagct aacatctctt cagattcggt tttcggtccg atggtgcaaa
    19741 caattttggt ctttttcatg acagtcttag tctttaagtt gagaaggatg ggagaaactt
    19801 gctttctggg cgcactgccg gaaagcgaat ccggtattac gaaagtggtg gtgcctcgct
    19861 ctaaggatag gtgacatcga aaaagcgtgc agaggaatgt gtgcttgtgt ctcagcccaa
    19921 cgtgagtgtc tgtttttgag tcgttgagtt ctacagtcca aagtgctgaa accattcaag
    19981 agtcaattgg cgcgcattat acgctgaaat taatccaaaa ggaaaacaaa aacagcgttt
    20041 caaaaaatat acaacttgtt agacaataaa ttgttatcaa agatttaacg ttccagggaa
    20101 aaggttacgc aaaaacttcg ctgtttgcgc aacgtctggt gccgaagttg tcagtgtgag
    20161 gataagtgaa agttcatccg gtgtataaat atatcatcgc tcaattgcta cagttaaaat
    20221 aatttatcgc gttatttgtc tgattttgtt tgatttttgt ttaattggtt agattgatat
    20281 atgctga
//
LOCUS       DADLTY010000075        20183 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.075, whole genome shotgun sequence.
ACCESSION   DADLTY010000075 DADLTY010000000
VERSION     DADLTY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.075"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="JN334_002215"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="JN334_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HAY7438347.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="JN334_002216"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="JN334_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HAY7438348.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="JN334_002217"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="JN334_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="JN334_002218"
     CDS             1631..2341
                     /locus_tag="JN334_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HAY7438349.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="JN334_002219"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="JN334_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HAY7438350.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4119)
                     /locus_tag="JN334_002220"
     CDS             complement(3469..4119)
                     /locus_tag="JN334_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HAY7438351.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4282..6480
                     /locus_tag="JN334_002221"
     CDS             4282..6480
                     /locus_tag="JN334_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY7438352.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6449..6889)
                     /locus_tag="JN334_002222"
     CDS             complement(6449..6889)
                     /locus_tag="JN334_002222"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7438353.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6886..7449)
                     /gene="tag"
                     /locus_tag="JN334_002223"
     CDS             complement(6886..7449)
                     /gene="tag"
                     /locus_tag="JN334_002223"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAY7438354.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7607..8305
                     /locus_tag="JN334_002224"
     CDS             7607..8305
                     /locus_tag="JN334_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7438355.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8534..9742
                     /locus_tag="JN334_002225"
     CDS             8534..9742
                     /locus_tag="JN334_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7438356.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10066..11757
                     /gene="eptB"
                     /locus_tag="JN334_002226"
     CDS             10066..11757
                     /gene="eptB"
                     /locus_tag="JN334_002226"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HAY7438357.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11849..11925
                     /locus_tag="JN334_002227"
     tRNA            11849..11925
                     /locus_tag="JN334_002227"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11883..11885,aa:Pro,seq:cgg)
     gene            12669..14276
                     /gene="dppA"
                     /locus_tag="JN334_002228"
     CDS             12669..14276
                     /gene="dppA"
                     /locus_tag="JN334_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HAY7438358.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14584..15603
                     /gene="dppB"
                     /locus_tag="JN334_002229"
     CDS             14584..15603
                     /gene="dppB"
                     /locus_tag="JN334_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HAY7438359.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15613..16515
                     /gene="dppC"
                     /locus_tag="JN334_002230"
     CDS             15613..16515
                     /gene="dppC"
                     /locus_tag="JN334_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HAY7438360.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16526..17509
                     /gene="dppD"
                     /locus_tag="JN334_002231"
     CDS             16526..17509
                     /gene="dppD"
                     /locus_tag="JN334_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAY7438361.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17506..18510
                     /gene="dppF"
                     /locus_tag="JN334_002232"
     CDS             17506..18510
                     /gene="dppF"
                     /locus_tag="JN334_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HAY7438362.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18540..19811)
                     /locus_tag="JN334_002233"
     CDS             complement(18540..19811)
                     /locus_tag="JN334_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY7438363.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4836 a         4995 c         5308 g         5044 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggcaggc
     4561 ggttataccg ggcatctggg ggattattcg accggtgcgg cacaggcgat catgccgtat
     4621 gtcgtaggtg gcagcgaagt ttatcaacag caaaccagtt ggccgctggt gctggaacat
     4681 agcgatgtcg tggtgttgtg gagtgctaac ccactcaata cgctgaaaat tgcgtggaat
     4741 gcatccgatg agcaggggct ttcttacttt tccgcactgc gtgacagtgg gaaaaagctg
     4801 atctgcattg atccaatgcg atcggaaacc gtcgatttct ttggcgataa aatggagtgg
     4861 gtggcaccgc acatgggcac cgatgttgcg ctgatgctgg ggatcgccca tacgctggtg
     4921 gaaaatggtt ggcacgacga agcgtttctg gcgcgttgca ccacaggtta tgccgtcttc
     4981 gcctcttatt tgctgggcga gagtgacgga atagcgaaaa acgccgaatg ggcggcagag
     5041 atttgtggtg ttggcgcagc gaaaatccgc gagctggcgg ctattttcca ccaaaatacc
     5101 accatgctga tggcaggctg gggaatgcag cgccaacagt ttggtgagca aaaacactgg
     5161 atgatcgtca cgctggcagc aatgttgggg caaatcggca cacccggcgg cggttttggt
     5221 ctttcttacc attttgccaa tggtggtaac cccacgcggc gttctgcggt gctctcttcc
     5281 atgcagggca gcttgccggg tggctgcgat gcggtggata aaatccctgt tgcccgcatt
     5341 gttgaagcac tggaaaaccc tggtggcgca tatcaacaca acggtatgaa ccgacatttc
     5401 ccggatattc gttttatctg gtgggcgggc ggtgccaact ttactcatca tcaggatacc
     5461 aatcgtctga tccgtgcctg gcaaaaaccg gagctggtgg tgatctctga atgcttctgg
     5521 acggcggcgg cgaaacacgc ggatattgtt ctgcctgcga ctacctcttt tgagcgtaat
     5581 gatctcacca tgaccggtga ttacagtaat cagcatctgg tgccgatgaa gcaagtggtg
     5641 ccgccacgct atgaagcgcg taatgatttt gatgtttttg ccgagttgag tgaacgttgg
     5701 gagaagggcg gctatgcgcg gtttacggaa ggaaaaagtc agctgcaatg gctggaaacg
     5761 ttttataacg ttgcccggca gcgcggggca agccagcagg ttgaattgcc gccatttgct
     5821 gagttctggc aagccaacca gttaattgag atgccggaaa acccggacag cgagcggttt
     5881 attcgcttcg ctgatttttg ccgcgatccg ctggcgcatc cgttaaaaac cgccagcggc
     5941 aagattgaaa tcttctcaca gcgtattgcc gattacggtt acccggattg ccctgggcat
     6001 ccaatgtggc tggagccgga cgaatggcag ggcaatgccg aaccagaaca gttgcaggta
     6061 ctttctgccc atccggcgca ccgcctgcac agccagctga attacagttc tctgcgcgaa
     6121 ttgtacgcgg tagcaaaccg tgagcctgtc accattcatc ctgacgatgc tcaggcgcgc
     6181 ggcataaccg aaggggatat ggtgcgggtg tggaactccc gtgggcagat ccttgccgga
     6241 gcggtcatta gcgagggaat taaacctggc gtgatttgca ttcacgaagg ggcatggccg
     6301 gatctggatt taaccgctga cggtatttgt aaaaacggcg cagtgaacgt gctgaccaaa
     6361 gatctcccca gctcgcggct ggggaatggc tgtgcgggta atacgacgct ggcatggctg
     6421 gaaaaataca acggtccgga actgacactt acagcgtttg aaccaccggc cagctcataa
     6481 tccaggtggg tagttgggtt tcatcctgcc atgcgcaatc gacaatgtga aaaccctgcg
     6541 cacggtaaaa atctatcgcc ggttgatttt tttgataaac ctccagcatc agatggggat
     6601 agcgctgctg cacatactgc atcagcgcct taccaatacc gcgtctgacg gccttcggtg
     6661 cgacaaacat tgctgccaga aatcggcctt ccataatgct gacaaaaccg agaagcttac
     6721 cgtcctcttc ccagacccag ttttgcgcgt tggcaagata ggcatcccgc accagcggaa
     6781 tgcagtcacg ccagtaattc gcttttataa agggatgccc ccaggttgta ctttccagcc
     6841 acagttcgag gatcgcgggg agttctgaac gttgcgcttc ccgaatcatg gtttatttcc
     6901 cagatagcaa cagcagccaa ccacatgatc attcaccagc ccacatgcct gcataaagga
     6961 gtaacagatt gtggtgccga caaacttaaa accacgtttt ttcagtgcct tagatagggc
     7021 gtcggaggcg gacgtagatg tgggaatttc gctcaacgtt gtggcttgtg tcacctgtgg
     7081 ctgatgattt acaaacgacc agacaaagtc gggaaacggt tcgccgttct gttccatttg
     7141 caggtacgcc cgcgcattac caataattgc ctgaattttc cctcgatggc ggataatccc
     7201 ggcgtcctgt accagtcttt cgacatcctc ttcctgcatt gctgcgacct tcaccggatc
     7261 gaactgatga aagtaggcgc gatagttttc gcgttttttg aggacggtga tccacgataa
     7321 cccagcctgc tgcccttcaa agcagatcat ttcgaacagt tttttactgt cagtttcagg
     7381 cacgccccac tcattatcat ggtaggcaat ataaagcggg ccctgactca cccagccgca
     7441 acgttccata ctttccctcg ctatgtaccc gatcgtaaaa atttcagccg cttttcttga
     7501 cgcatcggtg aaaaagtcaa tatttaatac agggctacta tgcccgatat ccttcataca
     7561 atgacaataa tattgccgaa ttcggctctt ccctggagtc gtgttaatga tcataaaaaa
     7621 aagcggtggt cgctggcagc taagcctgct ggcgagcgtg gtaatcagtg ccttttttct
     7681 caacacagct tacgcctggc aacaagaata tatcgttgat acgcaacccg gacattccac
     7741 agagcgttac acctgggata gtgatcatca acctgattac aacgatattt tgtcgcaacg
     7801 tattcaaagt agccaaaggg cgctgggact ggaagtcaat ctggcggaag aaactcctgt
     7861 ggatgtgacc agcagtatga gtatgggctg gaattttcct ttatatgaac aggttacaac
     7921 cggcccggtc gcggcattac attacgatgg cacaaccacc tcgatgtata acgagtttgg
     7981 cgacagtact accacgctga ccgatccgtt atggcatgcc agcgtgagta gcttaggctg
     8041 gcgtgttgac tcccggcttg gcgatctccg accctgggcg caaatcagct ataaccagca
     8101 atttggcgag aatatctgga aggcgcaatc aggcctgagc cggatgacgg cgacaaacca
     8161 gaacggcaac tggctggatg tcaccgtagg cgctgatatg ttgctcaatc aaaatattgc
     8221 cgcctatgct gcgctaactc aggcagaaaa taccactaat aatagcgact atctgtatac
     8281 gatgggggtt agcgccagat tttaacgtaa cagtcacaat tgaaaccatt gaataacaac
     8341 agttgtggcg atagtgggtg ctaacttacc aaataataaa tttggtgaat aattgtcgcg
     8401 tcattcattc ctgaactaag gcatttcatt ccgttctgat ggcatttcat gccgtttttc
     8461 cccaagcata aaatgcactt cgttatggtt gtcggcagag atttttcctt tttattactg
     8521 caggaatact gccatgacac cttcaaatta tcagcgtacc cgctggctga cactcatcgg
     8581 tactatcatt acccagtttg cgctggggtc ggtttatacc cggagcctgt ttaatggcgc
     8641 gctttctgcc aagctggatg cgccggtaag ccaggtcgct ttctctttcg gcttgttaag
     8701 tctggggctg gcaatttcgt cttctgttgc gggcaaattg caggaacgtt ttggcgttaa
     8761 acgcgtcacc gtggcttccg gcattttgct gggattaggc ttcttcctga ccgcgcattc
     8821 caacaacctg atgatgctgt ggttaagcgc cggtgtgctg gtcggtctgg cggatggcgc
     8881 gggttacctg ctgacgctct ctaactgcgt gaagtggttc ccggagcgta aaggtctgat
     8941 ctccgcgttc gctatcggtt cttatggtct gggtagcctg ggtttcaaat ttatcgacac
     9001 gcagctgctc gaaacggtgg gtctggaaaa aacctttgtg atttggggag cgattgcgct
     9061 ggtgatgatt gttttcggcg caacgttaat gaaagacgca ccaaaacagg aagtgaaaac
     9121 cagcaatggt gtggtggaga aagattacac gctggcagag tcgatgcgta aaccgcagta
     9181 ctggatgtta gcggtaatgt tcctgactgc ctgcatgagc ggcctgtacg tgattggggt
     9241 agcgaaagat atcgcccaaa gtctggcaca ccttgatgtg gtttccgcag ccaatgcagt
     9301 aacggttatt tccatcgcta acctttcagg tcgtctggtg ctgggcattc tgtctgacaa
     9361 aattgcccgt atccgtgtta tcaccattgg tcaggtgatt tcgctggtgg gtatggcggc
     9421 cctgctgttt gcaccattaa atgcagtgac gttctttgca gcgattgcct gcgtggcatt
     9481 taactttggc ggcactatta ccgtctttcc atcactggtc agtgagttct tcggcctcaa
     9541 taacctggcg aaaaactacg gtgtgattta cctcggtttc ggtatcggta gcatttttgg
     9601 ttcgattatc gcctcactgt ttggcggttt ctatgtgacc ttctacgtga ttttcgccct
     9661 gctgattctg tcattggcgc tttctacgac gattcgtcag ccagagcaga aaatgttgcg
     9721 tgaggcgcat ggctcccttt aatgctcagc cgcgagctac tgggcttcag cttccgtgtg
     9781 catcaaacgc atcaacgaaa tgtataaaca atgactcgta aaatttgggc cagttaactg
     9841 gcccttttta ctgctttttg taacgctaaa gtctcttttc aaacttgcat ttttgtaaat
     9901 ttgtgcttca tgcacactct ttccccacac tttttcgctt tgctgtggtc tacttattcg
     9961 cgcgtgtaga ttttacttat ctgactacct ccgcactttt cccctgccgg gcctgaaaag
    10021 ccgctaagca gggtgttatc acctgtttgt ccagggtttg tttgcatgag atacatcaaa
    10081 tcgattacac agcagaagct gagctttttg cttgcaatct atattggcct ttttatgaat
    10141 ggcgcggttt tttatcgccg cttcggcagc tatgcgcacg attttaccgt ctggaaaggc
    10201 gtttctgctg ttgttgaact ggccgccacc gtactggtga ccttcttttt actacgtctt
    10261 ctatcgctgt ttggccgccg cagctggcgt attctggcgt cgctggtggt gctcttttcc
    10321 gcaggtgcca gctattacat gaccttcctt aatgtggtca ttggttatgg catcatcgct
    10381 tccgtcatga ccaccgatat cgacctgtca aaagaagttg ttggtctgaa ctttattctc
    10441 tggttaatcg ccgttagtgc attgcctctt atccttatct ggaataaccg ctgtcgctac
    10501 accttgctcc gacaactgcg aaccccaggg cagcgtattc gcagcctggt ggtcgtcgta
    10561 ctggcgggta ttatggtttg ggcaccgatt cgtttgctgg atatccagca gaagaaagtg
    10621 gagagggcga ccggcgttga tttgccgagt tatggcggtg tcgtagcgaa ctcttatctg
    10681 ccatcaaact ggctttctgc gttggggctg tatgcctggg cgcgggtcga tgaatcttcc
    10741 gataataatt cattgcttaa tccggcgaag aagtttacat accaggctcc acaaaacgtt
    10801 gatgatactt atgtcgtgtt tatcatcggt gaaaccacgc gttgggacca tatgggtatt
    10861 ttcggctatg agcgtaatac cacgccgaaa ctggcccagg agaaaaatct ggcggcgttc
    10921 cgtggttact cctgtgatac cgcaaccaaa ctctcactgc gttgcatgtt tgtacgtcag
    10981 gggggcgcgg aagataatcc gcagcgcaca ttaaaagaac agaacatttt cgcggttctg
    11041 aagcagttag gattcagttc tgacctctac gctatgcaga gcgaaatgtg gttctacagc
    11101 aacacgatgg cagacaacat tgcttatcgt gagcagattg gtgcggagcc acgtaatcgt
    11161 ggcaagccgg tagatgatat gttgctggta gacgaaatgc agcaatcgct agggcgcaac
    11221 ccggatggta agcatctgat cattctgcat accaaaggtt cgcattttaa ctacacccag
    11281 cgttatccgc gtagcttcgc gcagtggaag ccggaatgta ttggtgttga tagcggctgt
    11341 accaaagcgc agatgatcaa ctcctatgac aactcggtga cctatgtgga tcactttatc
    11401 tccagcgtga ttgatcaggt tcgcgataag aaagcgattg tgttctacgc agctgaccac
    11461 ggtgagtcaa ttaatgaacg cgagcacctg cacggcacgc cgcgtgaact ggcaccgccg
    11521 gagcagttcc gcgtaccgat gatggtctgg atgtcagata aatatctgga aaatccggcc
    11581 aatgcgcagg cgtttgcgca gctgaaaaaa gaagccgaca tgaaagtgcc acgccgtcac
    11641 gtagagctgt acgacaccat catgggttgt cttggctata cttcaccgga tggtggaatt
    11701 aacgaaaaca acaactggtg tcacatcccg cagacaaaag tggcagcggc taactaagcg
    11761 gcctgctgac tttctcgccg atcaaaaggc attttgctat taagggattg acgagggcgt
    11821 atctgcgcag taagatgcgc cccgcattcg gtgattggcg cagcctggta gcgcacttcg
    11881 ttcgggacga aggggtcgga ggttcgaatc ctctatcacc gaccaaattc gaaaagcctg
    11941 ctcaacgagc aggttttttt gcatttgtag ctcatgagga tgagaacctc cgggggcagg
    12001 gaggctcgac tcgacgtagg cctgataaga cgcgccagcg tcgcatcagg caacggcacc
    12061 gcacttattg tcggatgcgg cgcgaccgcc ttattcgacc aacacaccac acattttcct
    12121 gccaactttg cgccccatat ctcactatcc attaacggat ttgtgacaga aaccattgtc
    12181 tttttttata tttgcttaaa tcttttttgc gttacttatg gctggcgctg gcattttccg
    12241 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
    12301 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
    12361 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
    12421 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
    12481 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
    12541 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
    12601 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
    12661 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
    12721 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
    12781 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
    12841 tcccgcttta taaccgcctg gttgaattta aaatcggcac caccgaagtg atcccgggcc
    12901 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
    12961 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg cgaaatgaac gccgatgacg
    13021 tggtgttctc gttcgatcgt cagaaaaacg cgcaaaaccc gtaccataaa gtttctggcg
    13081 gcagctacga atacttcgaa ggtatgggct tgccggagct gatcagcgaa gtgaaaaagg
    13141 tggacgataa caccgttcag tttgtgctga ctcgaccgga agcgccgttc ctcgctgacc
    13201 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgacgcgatg atgaaagccg
    13261 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
    13321 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
    13381 agatcgatac gctggtcttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
    13441 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
    13501 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcgtata
    13561 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca agcactaacc tacgcggtga
    13621 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
    13681 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
    13741 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
    13801 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
    13861 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    13921 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    13981 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    14041 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    14101 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcagg
    14161 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    14221 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    14281 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    14341 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    14401 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    14461 cctctgcgtg cagcgccatc cggcagcaat acttcattct ctgtccgaca agggcgggaa
    14521 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg agtcactata gagaatccgg
    14581 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    14641 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    14701 ggcgaacgtg ggatctcccc tgagcgtcac gtgcagctgc tggctgaact cggcttagat
    14761 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    14821 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    14881 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    14941 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggctt ggcgctgacc
    15001 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    15061 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    15121 ccgttaaccg gttttatgct aatcgacacc gccatctggg gtgaagacgg caactttatc
    15181 gatgccgtcg cccatatgat cttgcccgcc attgtgctgg gtactattcc gctagcggtc
    15241 attgtgcgta tgacacgctc ctcgatgctg gaagtgctgg gcgaggatta catccgcacc
    15301 gcgcgcgcca aagggctaac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    15361 atgctgccgg tagtgaccgt tatcggcctc caggtgggaa cattgctggc gggggcgatt
    15421 ctgaccgaaa ccatcttctc gtggcccggt ctggggcgct ggttgattga cgcactgcaa
    15481 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    15541 gtcaacttgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    15601 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    15661 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt cgtcgggctg
    15721 gtttacgtcg ccatcgtgct gttcatcgcg atctttgcca actggattgc gccttataac
    15781 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagt
    15841 atggcacact tgctgggcac cgatgacgtc ggtcgtgatg tgctgtcgcg gctgatgtac
    15901 ggcgcgcgtt tatcgctgct ggttggctgt ctggtggtgg tgttgtcgct gattatgggc
    15961 gttattctcg gcctgatcgc cggttacttc ggcggcctgg ttgataacat tatcatgcgc
    16021 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    16081 ttcggcccgt cgattggtaa cgccgcgctg gcgctgacct tcgttgcctt gccgcactat
    16141 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt caccgcctct
    16201 cgcgtggcag gtgccggggc gatgcgccag atgtttatta acatcttccc gaactgcctt
    16261 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggctgct
    16321 cttggtttcc tcggcatggg ggcacagccg ccaacgcctg agtggggcac catgctctcc
    16381 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttccctgg tctggcgatc
    16441 ctgttgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    16501 aaactaaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    16561 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    16621 gggcgaagtg gtcgggattg tgggtgagtc cggctccggt aagtcggtca gttcactggc
    16681 gattatgggg ctgattgatt atccgggccg cgtaatggca gaaaaactgg agtttaacgg
    16741 ccaggatttg cagcgtatct cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    16801 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    16861 tatggaagcg attaaagtgc atcagggcgg caacaaaagt acccgccgtc agcgggcgat
    16921 tgacctgctg aatcaggtcg gtattcccga tccggcttcg cgtctggatg tttacccgca
    16981 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    17041 aaaactgctg attgccgatg aaccgaccac cgcgctggac gtgaccattc aggcgcaaat
    17101 catcgaacta ctgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    17161 tgacctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggtcaggt
    17221 agtagaaacg ggcgatgcac acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    17281 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    17341 tgttgttccc ggcaagtatg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    17401 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg atgggcgtca
    17461 gtccaaatgc cattacccac ttgatgatgc cgggaggccc acactatgag tacgcaagag
    17521 gccacctcgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    17581 aaaggcatgt tcgtgccgga acgtctggtg aaggcgctgg acggcgtttc gtttaacctt
    17641 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    17701 cggttgctga cgatgatcga aacgcccacc ggcggcgagc tgtattatca ggggcaggat
    17761 ctgctcaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    17821 cagaatccct acggttcgct gaatccgcgt aagaaagttg ggcaaattct cgaagagccg
    17881 ctgcttatta acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    17941 gcaaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcacatgtt ttccggcgga
    18001 caacgtcagc gtatcgctat tgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    18061 gatgagccgg tttctgcgct ggacgtctca gtgcgtgcgc aggtgctgaa tctgatgatg
    18121 gatttgcagc aggagttggg gctttcttat gtctttatct cccacgacct gtcggttgtg
    18181 gagcacattg ctgatgaagt gatggtgatg tacctaggcc gctgcgtgga gaaaggaacg
    18241 aaggaccaaa tcttcaataa cccgcgccat ccgtacaccc aggcgctgct ctccgcgacg
    18301 ccacgcctga acccggacga tcgtcgtgaa cgcatcaaac tcaccggtga actgccaagc
    18361 ccgctgaatc ctccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    18421 tgcacccagt tgcagccgca gctaaaagac tacggaggtc aactggtagc ttgttttgct
    18481 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    18541 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtaaag cagaatcagc
    18601 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    18661 atgaggcacg acacaatgcc atataacggg cttcccaact gaaagaacac cagcactgat
    18721 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    18781 agcatgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    18841 cctttaatag cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    18901 atattcagca ccgtagaggt gatatgaatg atatgtccag ggatcacctg cgcggccagt
    18961 gccagtgccg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    19021 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    19081 gtacgcagcg cgagccgggt tgccagtact ttatctgctt cccgtttacg ataggcaata
    19141 ttcattgggt taagtacctg aataaatacc gcagaaaaga agcaaaatgg aatggtaagc
    19201 aggacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    19261 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    19321 ccagaaatct taaacagtaa tcgttcacca ccagacgcaa tcgctatcag tacggcgaaa
    19381 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    19441 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    19501 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    19561 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    19621 actacccagg tgacaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    19681 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    19741 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    19801 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    19861 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    19921 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    19981 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    20041 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agcaatcgta gaatcagcgt
    20101 caagccggaa ggattccggt gaggcgcaat gttgcggggg cttaatccct ggtggcattg
    20161 gttgctggag agagaaaacc ccc
//
LOCUS       DADLTY010000076        19684 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.076, whole genome shotgun sequence.
ACCESSION   DADLTY010000076 DADLTY010000000
VERSION     DADLTY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.076"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            206..1303
                     /gene="mltA"
                     /locus_tag="JN334_002234"
     CDS             206..1303
                     /gene="mltA"
                     /locus_tag="JN334_002234"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAY7438364.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1541..2347
                     /gene="tcdA"
                     /locus_tag="JN334_002235"
     CDS             1541..2347
                     /gene="tcdA"
                     /locus_tag="JN334_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HAY7438365.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2398..2841)
                     /gene="csdE"
                     /locus_tag="JN334_002236"
     CDS             complement(2398..2841)
                     /gene="csdE"
                     /locus_tag="JN334_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HAY7438366.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2841..4046)
                     /gene="csdA"
                     /locus_tag="JN334_002237"
     CDS             complement(2841..4046)
                     /gene="csdA"
                     /locus_tag="JN334_002237"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HAY7438367.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4238..4465
                     /locus_tag="JN334_002238"
     CDS             4238..4465
                     /locus_tag="JN334_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAY7438368.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4816..5733
                     /locus_tag="JN334_002239"
     CDS             4816..5733
                     /locus_tag="JN334_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HAY7438369.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5752..6147
                     /locus_tag="JN334_002240"
     CDS             5752..6147
                     /locus_tag="JN334_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HAY7438370.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6140..7240
                     /gene="rlmM"
                     /locus_tag="JN334_002241"
     CDS             6140..7240
                     /gene="rlmM"
                     /locus_tag="JN334_002241"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HAY7438371.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7284..8015)
                     /gene="fucR"
                     /locus_tag="JN334_002242"
     CDS             complement(7284..8015)
                     /gene="fucR"
                     /locus_tag="JN334_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HAY7438372.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8073..8495)
                     /gene="fucU"
                     /locus_tag="JN334_002243"
     CDS             complement(8073..8495)
                     /gene="fucU"
                     /locus_tag="JN334_002243"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HAY7438373.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8497..9916)
                     /gene="fucK"
                     /locus_tag="JN334_002244"
                     /pseudo
     CDS             complement(8497..9916)
                     /gene="fucK"
                     /locus_tag="JN334_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(10025..11800)
                     /gene="fucI"
                     /locus_tag="JN334_002245"
     CDS             complement(10025..11800)
                     /gene="fucI"
                     /locus_tag="JN334_002245"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HAY7438374.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11833..13149)
                     /gene="fucP"
                     /locus_tag="JN334_002246"
     CDS             complement(11833..13149)
                     /gene="fucP"
                     /locus_tag="JN334_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HAY7438375.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13696..14343
                     /gene="fucA"
                     /locus_tag="JN334_002247"
     CDS             13696..14343
                     /gene="fucA"
                     /locus_tag="JN334_002247"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAY7438376.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14371..15519
                     /gene="fucO"
                     /locus_tag="JN334_002248"
     CDS             14371..15519
                     /gene="fucO"
                     /locus_tag="JN334_002248"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAY7438377.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15572..16417)
                     /gene="xni"
                     /locus_tag="JN334_002249"
     CDS             complement(15572..16417)
                     /gene="xni"
                     /locus_tag="JN334_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HAY7438378.1"
                     /translation="MRSLFLFSQPAIACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKADRPP
                     MPEELHDEMPALRTAFEQRGVPCWSASGNEADDLAATLAVKVTQAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(16438..17805)
                     /gene="sdaB"
                     /locus_tag="JN334_002250"
     CDS             complement(16438..17805)
                     /gene="sdaB"
                     /locus_tag="JN334_002250"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HAY7438379.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17863..19152)
                     /locus_tag="JN334_002251"
     CDS             complement(17863..19152)
                     /locus_tag="JN334_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY7438380.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4861 a         4815 c         5194 g         4814 t
ORIGIN      
        1 gtggatgcgc actatactcc gctcgcgttt tcatgcaacc ttttttttac cccgatcacg
       61 gatttagcat gattttgaac aaaaacaaac cactaaagcc tattttttgt gcaaaatttg
      121 cgtcagggag aggctgctca tttcattaaa cgcggcggtt tgttatcttc gttgcgcctt
      181 attttttaac ctgaagaaga gaacaatgaa aggacgttgg gtaaagtacc ttcttatggg
      241 cacggttgtg gcaatgcttg ccgcctgctc ttccaaacca accgatcgcg gacagcaata
      301 taaagacggg aaatttaccc agcctttctc tctggtgaac cagccagatg ccgttggcgc
      361 gccgattaac gccggtgatt ttgccgagca aattaaccat atccgtaatt cgtcaccgcg
      421 tctgtatggc aaccagagta atgtttataa cgcggtgcaa gagtggctgc gcgcaggcgg
      481 tgatacccgc aatatgcgcc agttcggcat tgatgcctgg cagatggaag gtgccgacaa
      541 ctatggtaac gtgcagttta caggctatta cacgccggta attcaggcgc gccatacccg
      601 ccagggcgag ttccagtatc ctatttaccg tatgccgcca aaacgtggtc gtctgccgtc
      661 tcgtgcggag atctacgccg gggcattggg cgataaatac atcctcgctt acagcaactc
      721 tctgatggat aacttcatta tggatgtgca gggtagtggg tatatcgact ttggtgatgg
      781 cagtccgctt aactttttca gctatgcagg gaaaaacggt catgcctatc gcagcattgg
      841 taaggtgctg atcgaccgtg gcgaagtgaa aaaagaagat atgtcgatgc aggcgattcg
      901 tcattggggc gaaacacaca gtgaagccga ggttcgcgag ctgctggaac agaacccgtc
      961 tttcgtcttc tttaaaccgc aatcttttgc accggtgaaa ggggcaagtg cggtgccgct
     1021 ggttggccgt gcttctgttg cctccgatcg ttcaattatt ccgccaggta ctaccttgct
     1081 ggcagaagtg ccgctgctgg ataataacgg caaatttaac ggtcagtacg aattgcgtct
     1141 gatggtggcg ttggatgtcg ggggcgcaat caaaggccaa cacttcgata tctatcaagg
     1201 gatcgggccg gaagccggac accgcgcagg ttggtacaac cactatggac gggtttgggt
     1261 gctgaaaacg gctccgggcg caggtaacgt ctttagcggc tgatgtgata ttctgtatgc
     1321 cgtttttacg gatatgacag gggaggggta acctcacatc ctgattattg actaagtgcg
     1381 cgttgttcat accggatgcg gcgtgaacgc cttatccggc ctacaaatca tgcaaatcca
     1441 aaatattgca ggagttatgt aggcctgata agcgtagcgc atcaggcatt tatgcgttta
     1501 gggtgaggga taacctcgcc ctttttaatt ctgaggtgct atgtctgtgg taattagtga
     1561 tgcatggcgt cagcgttttg gtggcacagc gcgtctgtat ggtgaaaaag cgttgcaact
     1621 gtttgctgac gcgcatattt gtgtggttgg tatcggtggt gtcggttcct gggcggcgga
     1681 agcgctggcg cgcacgggga ttggcgcaat cacgcttatc gatatggatg atgtgtgcgt
     1741 caccaatacc aatcggcaaa ttcacgctct gcgcgataac gtcgggctgg caaaagcgga
     1801 agtcatggcg gagcgcattc gccagattaa cccggagtgc cgtgtaacgg tggtggatga
     1861 tttcgtgacg ccggataacg tagcgcaata tatgagtgcc ggttattcgt acgtgattga
     1921 tgccattgat agtgtacggc ccaaagcggc gctgattgct tattgtcggc gcaataaaat
     1981 cccgctggtc acgaccggtg gcgcgggtgg gcagattgac ccgacgcaga ttcaggttac
     2041 cgatctggcg aaaacgattc aggatccgct ggcggcgaag ttgcgcgagc gcctgaaaag
     2101 cgattttggc gtagtgaaaa acagtaaagg taagctcggc gtggattgcg tgttttctac
     2161 tgaagcgctg gtgtacccgc agtcagacgg tacggtgtgt gcgatgaaag ccacggcaga
     2221 agggccgaag cggatggatt gtgcatctgg atttggcgcg gcaacgatgg tgaccgccac
     2281 ctttgggttt gttgcggttt ctcatgcgct gaagaagatg atggcgaaag cggcgcgtca
     2341 gggttaaaca atcaggtcgg atgagacgcg aacgtcttat ccgacccggt tctgtgctta
     2401 aacctgcttc gtcgcagcga taatcgcctc gcttaacgca tttaacccct ggctgcgtga
     2461 ggcgctaagc tgcgcacgta atcccagctc atcaaacaat gccagcggcg actgtgcctg
     2521 taattcggcg gcggttttcc cctcaacggc agtcaacaac accgccaaca ggccgcgcac
     2581 aatgcgccct tcgctgttgc caaagaagtg cattttgccg ttttcagcca cggtatatcc
     2641 cagccagacg cggttttcgc atccggcaat ctctttagcc tgcgctttta actcgtctgg
     2701 caaagccgga agctgtttcc ccagcatgat caactggcga tatttatctt cccattgcga
     2761 caacggtgcg aaggtattgc gtaacgtttc tgcggttacg gttgtgccga acggatgtcc
     2821 ggcgaattgc gggtttgtca ttaatccacc aataattcca gcgcgcggtc aacggcattc
     2881 accagcgcat ccacatcact ctttgtatta tatggcgcaa atgaggcgcg cagtgtgccg
     2941 gttacgccta attctgccag tagcggctga gcgcaatgtt gcccggcccg caaggcaata
     3001 ccgtactccg ccagtagcgt caccatatcg ctgtgatgaa cgccagcaaa atcaaaggcc
     3061 agcaggctgg aatcctggca gcggaatgaa cgaaagcctg gacgtttcgc cagcgcttct
     3121 tctgcaagcg ttgctaagct acggctccag ctttcggcct ggttgatatc gtaatctgcc
     3181 agccattcca gcgccgcgct taatcctatg acgccagcga catttggcgt tcctgcttcc
     3241 agtttccacg gcgcagattg agtggtgaaa ccgtcaaaac tcacttcgtg aatcattttg
     3301 ccgccaccca gccagggcga catcgcttcc agcagttctg atttgccata cagcacgccg
     3361 atacccgtcg ggccatacag tttgtgacca gagaaagcat agaaatcgat atccagttgc
     3421 tgaacatccg cggggaaatg caccgtcccc tgagcaccat caaccatcac caccatcccg
     3481 gctgaatgag caaaggtaat cgctcgcgcc agatccgggc aaccgccagt cacgttcgac
     3541 atctgaccca acgccagaat ccgactacgg ggagtaatca gctctggcaa caaatcgaca
     3601 tccggcagtc gctgcgcatt aagcggcaat ttcaccactt tggctctagt ttgttgggcg
     3661 accatcagcc aggggacgag gttagcgtgg tgttctgcca cgctgacaat aatctcatcg
     3721 cccggttgca gacgcggacg cgcatagcat tgtgccacca tgttgatgga ttcagtggtg
     3781 ccgcgcgtcc agacgatagt tttatcatcc ggtgcattca gtaattgcgc cactttctct
     3841 cgtgcagctt cataacgcgc ggtcaggcgt tgggcttcgg caaactggct gcgatggacg
     3901 tttccggcgc tcaggctgta aaactgtcga gtggcttcaa ccacggcttc aggtttaagc
     3961 gcggtcgcgg cgctgtcgag atagacgccc gcatcctgta gtgcgggaaa ctgggcgcga
     4021 aactgcgcgg gattaaaaac gttcatggta ctcctcggct tgatgcaccg atcgtgacgc
     4081 aaaattcact ttgatacaag gagagtgcta accatcagaa ttatctggat atcctggcga
     4141 agccgcgtaa gtaacatatg ctgaatagtg ttaggcgtta attttagcct gttaatcaat
     4201 actattaaga tttattaatc gttttaagga gaagatgatg aaaaagactg ccgcaattat
     4261 ttctgcctgt atgctgactt ttgccctgag cgcctgttcc ggttcgaact atgtgatgca
     4321 caccaatgac ggacgtacca tcgtctctga cggcaaacca cagactgata acgatatcgg
     4381 tatgatttcg tataaagacg ctaatggcaa caaacagcag atcaaccgta ctgacgtgaa
     4441 agagatggtc gaactggaac agtaattcgc gatcgcaagg taaaaaaaag caccgcaatt
     4501 aggcggtgct acattaatca ctatggacag acagggtaaa tgtacaggaa gtgaaaaaag
     4561 gtagctttgc taccatggtc tgaatcgcag accaattgca aacacaacaa cacaacatca
     4621 caaccgtaag ccaaaagttc accagaatac gcattccgat aaaacttttc gttccggctc
     4681 aggaagtgcc gccactatag gtatttgctg gtagaagctc aacggacaat ttataatggc
     4741 tcagattaaa aaaactaata ggttacacag tgtgatctaa ttgttaaatt catttaacat
     4801 caaagtttaa aagccatgtc taaacgatta ccaccgctaa atgccttacg agtttttgat
     4861 gccgcagcac gccatttaag ttttactcgc gcagcagaag agctttttgt gacccaggcc
     4921 gcagtaagtc atcaaatcaa gtctcttgag gattttttgg ggctaaaact gttccgccgc
     4981 cgtaatcgtt cactcctgct gaccgaggaa gggcaaagct atttcctcga tatcaaagag
     5041 atattttcgc aattaaccga agcgacgcgt aaactccagg cccgtagcgc caagggggcg
     5101 ttgacggtca gtttactccc cagtttcgcc attcattggt tggttccgcg actttccagc
     5161 tttaattcag cttatccggg aattgacgtt cgaatccagg cggttgatcg tcaggaagat
     5221 aagctggcgg atgatgttga tgtggcgata ttttatggtc ggggcaactg gccggggcta
     5281 cgggtggaaa aactgtacgc cgaatattta ttgccggtgt gttcgccgct actgctgaca
     5341 ggcgaaaaac ccttgaagac cccggaagat ctggctaaac atacgttatt acatgatgcg
     5401 tcacgccgtg actggcagac atatacccga cagttggggt ttaatcatat caacgttcag
     5461 caagggccaa tttttagtca tagcgccatg gtgctgcaag cggctattca cgggcaggga
     5521 gtggcgctgg caaataacgt gatggcgcaa tctgaaatcg aggccggacg tcttgtttgc
     5581 ccgtttaatg atgttctggt cagtaaaaat gctttttatc tggtttgtca tgacagccag
     5641 gcagaactgg gtaaaatagc cgcctttcgc caatggatcc tggcgaaagc cgctgctgaa
     5701 caagaaaaat tccgctttcg ttatgaacaa taatttacgt agggtacgac catgaccagc
     5761 cgttttatgc tgattttcgc cgccattagc ggcttcattt ttgtggcact gggcgctttt
     5821 ggtgcgcatg tgttaagtaa aaccatgggg gccgttgaga tgggctggat ccaaaccggt
     5881 ctcgaatacc aggcgtttca tacgctggcg atcttaggtc tggcggtggc aatgcagcgt
     5941 cgcatcagta tctggtttta ctggagtagc gttttcctcg cgttaggcac ggtgctgttc
     6001 agcggcagcc tttattgcct ggcgctgtcc catctgcgtt tgtgggcgtt tgtcacaccg
     6061 gttggcggcg tgagcttcct tgcgggctgg gcgttaatgt tagttggtgc tatccgttta
     6121 aagcgcaagg gcgtaagtca tgaataaggt tgtattgctg tgccgtccgg gctttgaaaa
     6181 agagtgcgcc gcagaaatta ccgataaagc cggccagcgg gaaattttcg gttttgcccg
     6241 cgtgaaagag aatgcgggtt atgtcattta tgaatgttat caacctgatg atggcgataa
     6301 gttaatccgt gagctgccgt tcagttcatt aatttttgcc cgccagtggt ttgtggtggg
     6361 ggaactcctg cagcatttgc cgccagaaga tcgtattacc cccattgtcg gcatgttaca
     6421 gggtgtagta gagaagggcg gtgaactgcg tgttgaagtt gccgatacca acgaaagcaa
     6481 agagttgctg aaattctgcc gtaagttcac tgttccgcta cgcgctgcct tgcgcgatgc
     6541 tggggtgctg gcgaactatg aaacgccgaa gcgtccggtt gtgcatgtat tctttattgc
     6601 accaggatgc tgctataccg gttactcata cagcaacaat aattcgccgt tctatatggg
     6661 cattccgcgc ctgaaatttc cggcagaggc gccgagtcgt tccacgctca aactggaaga
     6721 ggcatttcat gtgtttattc ctgcggatga gtgggatgaa cgcctggcga acgggatgtg
     6781 ggcggtggat ttaggcgctt gccctggcgg ctggacctac caactggtga agcgcaacat
     6841 gtgggtttat tccgtcgaca acggcccgat ggcgcaaagt ctgatggata ccgggcaggt
     6901 gacgtggctg cgggaagacg gtttcaaatt ccgtccgaca cgcagcaata tctcctggat
     6961 ggtatgcgat atggttgaaa aaccggctaa agttgcggca ttgatggcgc agtggctggt
     7021 taatggctgg tgccgtgaaa ccattttcaa tctcaaactg ccgatgaaaa aacgctacga
     7081 agaagtatca cacaatctgg cgtatattca ggcacagctt gatgaacatg gcataaatgc
     7141 tcagattcag gcgcggcagt tgtatcacga tcgcgaagaa gtgacggtgc acgtccgccg
     7201 catctgggct gcggtgggtg gtcgtcgcga cgagcgataa caggcaaggc cggggcgtcg
     7261 tctccggcct gctacccttt ccgtcaggct gttaccaaag aagttgcaac ctggcgctca
     7321 tcagaaataa tgtgcgttac ctcatccaga tgcccgatgc gggcttcccc tgaacgatta
     7381 aatttacttt tatcaatcaa taacaacgat tgcgcggcac gttttaatag catcgatttg
     7441 taatcagcgt tgatcgcatt ggagtcccac agtgcgccgc tgctatcgat cccttcacaa
     7501 gaaaaaataa acagatcgat ttccagcgat ttaagttggg aaatcagcga gggattgacg
     7561 taacagccat atttgcgctc aagtgtgccg ccggaactga tcagttgaat gcgttcgcgt
     7621 ttaccgagtt catggcaaat cggatggcta ttggtgaaga cctgaatgtt gatgtcaggc
     7681 aactggcgtg ccagatacca gcaagttgaa ctggcgtcta aggctatcac catcccttct
     7741 tcaatccacg cgagcgcctc gcgcgcgata tctgctttat gcgcataatg gcttttcagc
     7801 ctgatgtgaa agggatcgcc actgtcttga ttttgacggt ggatatattt agcgcgtcca
     7861 tgattgcgca gaattttacc ctgcgtctgt aattcattga gatcgcgacg aatggtttct
     7921 ttactgacct ttagctgttc agagagagct tccgtggtca ggctggtatg gttcagcagc
     7981 aggtcgacta tcgcttgctg gcgtgccgct ttcatatttt tcaccccatc attcgataac
     8041 cgcccgcgtc atgcgggcgt accggtatga gattacggtg ttaccccttt ttttaaaaga
     8101 atattcccgt acttcgctcg ttcgcctgtg ataacgatcg caaaggcttt ttgcgcccgt
     8161 tcataaaacg caaaacgatt gatgcggatg atgtcaggac acggggcttg tagtgaaagc
     8221 gcattacggt aacgtcgttc tacttcagga tcgagagtgt caccctctac cgccgccatc
     8281 atcaccagcg gcggggcata actgtccagc tcaaataacg ggataatcgc ctggagcaag
     8341 tcgctcacca acaggccatc agcgcggatc acctgcggtt ccatcgaatg ggcgggaaag
     8401 tgagcatcgg aaaaaataat ttcatctcca tgtcccatct ctgccagcac tttcaatagt
     8461 tcgggagaaa ttaacggcga aattgttttc agcatttcac acttcctcta taaattcagg
     8521 ttcagtttgc gggtagaaat aacggtactg ataatgaatc tgtgcacggg cttcttccgg
     8581 gctgttaaat tcccctacgc cataccaacc gaacagcgca gctcctgcga cggtcgtttc
     8641 ggcatcgtcg agaactttta ccggaatatc aagcatattg gctttaatct gattccacaa
     8701 tgtgttgcga cttcctccac cgactaacaa taattcatag gctttaaagt gcccgatttt
     8761 ttccagcatc tgtagattgc gctgtaattg cgcagttaac ccttccagcg ccgcgcgata
     8821 gaaatgcccc cgcgtggtat taagcgtcac tccttgccag ccagcgttct gacacgacaa
     8881 taaatcacac tgcattttca caccatccgc gccaggtgcg atcagacgag cttcttcaat
     8941 caacatttgc cagggtgttt cagccgtcca gaacagtttt ctcacccatt ccagcacgcc
     9001 ggatgccagc cattgcatac ctgggttata caaccatgcc tggctatcta gttcgcaggt
     9061 ggaaccggcg tactgactta acagcgaagt atcaacctag gcgctgcgaa ccattaaaat
     9121 ttcccatgta ccggaggaga gcacgggttc attttgttcg gcaccagcgc caaaaagggc
     9181 gaactgggta tcgtgacctg cggaaatcac cggtatgcca acgggtaagc cgagcattgc
     9241 tgcggcgttg ttctgtagcg taccaatctg ttcacccgct tccaccagac gagggaagag
     9301 tcggcgtgga ataccggtgg cttgtaaaat ttgcggactg aaatcgcgtt gctggatatc
     9361 cagcatctgg ctggttccgg ccatcgtgat atcagtagtg aattcgctgg ttaaacggtg
     9421 gttaatcagc gacgaaatga agagccaggc gtgcgcgcgt tccagcagtt gtggatgatt
     9481 ttctttcaac cacaccaact tatataacgt attgaaacta aaggctccga cgccagaaat
     9541 agcctgcaac cgctgtgcgg agattaaccg ttcaatattg tccataaccg ctgctgttcg
     9601 cggacatttc cagctaatga tcggatagag cagattgcct tgcttatcta ccagagcgcc
     9661 atccacacta aaggtggtga cggcgatacc gcggatgtgg cattcagtca gttcactatt
     9721 gatttgccga cagcaatcag caaagcgttg caaaatggca gtctaaagac cactggtgcc
     9781 aggtgttgtt ttccatcgcg atatcgctgg cattaggcgt tgaggcgcgg gcaacaattt
     9841 ttccctgccg attaaccgcg atggccctga cattggtcgc gccacagtcg agtaccagga
     9901 taacttcttg tttcataatc gctcctgcaa tatagccgga taacattgtt tatccggcta
     9961 accactcttg cttgttcagg cccggtagcc ggagcgaccg ggcatcacat cagggtgtaa
    10021 tgtattaacg cttgtacaac ggaccgtagt tctggcaagc gcggtaatcc tggccttcaa
    10081 tatccatgcc gtgcgcagcc caggcagaag gacgatacac tttggtctct tcaacgttgt
    10141 gcatacatac cgggatacgc agcatggagg caagggtgat aaagtctgcg ccaacgtggc
    10201 cgatggtcag aactccgtgg ttggcacccc agttcgccat taccgaatac acatccgtaa
    10261 acggcccttt accagtgagg cgcggtgcaa accaggtggt tggccaggtt gagttggtgc
    10321 gtttgttgag gatgtcatgc acatccttcg gcaattccac gctccagcct tccgcgattt
    10381 gcagtaccgg tcccaggcct ttgatgatgt tgacacgagt catggtgaac gggacgccgc
    10441 cttcggtaag gaagcgggaa gagtaaccgc cgccacggaa gtattcgtgg atcgccgggc
    10501 accattcggt agcggcgagg caagcgtcag cctcttgctg agagatttcc cagtgcggct
    10561 tcatcgtcgg gttaccttcg ctgtcgcgtt gtttacagga accgtccagc gcagcagaac
    10621 ctgagttgat caaatggatg atgccgtgtt ctgccagtcc gtccagttta tgccccgtta
    10681 cacgctcaat tgcttctggt gaccagtagg tacgcacatc ggcaaatacc tgagcggtgc
    10741 cggtgagctg gtgacccatt agcattgcca cgccgttaag actgtcgttt tcggtcgcca
    10801 cgacaaaggg ttcgcgcacg ccattccagt caaatgaact gttgaggatc gcttcggcgg
    10861 tgtcaccatt gggatattga tcggtccagt gacgttgccc ctggaagccc gcagcgatgg
    10921 cgttgtagcc aagtgattct tccacgcgac caatatcggc cagtttgctg ttgccttgca
    10981 tcatgtcgcg gatacacatc gccatcagta aactttcgcg cagaactgcg cggctttgct
    11041 cggcattgcg ttgatactgt ttgttatttt catcttcgcc atagcggaag tttttatcag
    11101 cccaggccag tgccatttcc agttcggctt cgtcgtaaat cttctgatcg atacggcgac
    11161 gcagttcggt catatccacc gcctggactt tcattcccag ccaggattca aagaagttgt
    11221 gatcaacaat agaaccggcg atacccatcg aaacgccgcc cagcgacaga tagcttttac
    11281 ctttcatgct ggcgacggcc aaaccggcgc gggcaaagcg cagcagtttt tcttcaacat
    11341 cggcaggaat cgatgtgtca tcggcatcct gaacgtcatg accgtaaatg gagaatgccg
    11401 ggatgccttt ctggctgtga gctgccagag ccgcagccag gtaaacagcg ccggggcgtt
    11461 cagtgccgtt aaagccccaa atggctttcg ggcgggttgg atccatgtcg atggtttcac
    11521 tgccatagca ccagcaaggc gttaccgtaa tggtgaggcc tacattctgg ctgctgaatt
    11581 tttcttcgca agcagcggct tcagccatac ccgcgataca ggtatcggaa atgacacact
    11641 cgacggcagc tccgcaggca tggcgcagtt tctcggtcag cagtgcggcc gtagctttcg
    11701 ccatattcat tgtttgttct tcaagcgact cacgaacacc catgcgacga ccgtcaataa
    11761 ccgggcggat accaattttc ggtaagctga tttttttcat tacagattcc tcactttatt
    11821 cggaaaatat gttcagttag ttgccgtttg agaacggaaa cgggcaaaga taaagatgac
    11881 cgcgaagcag agtgcgggga tcagttcagc agtggggatg ttgcccgccg cgtcactgac
    11941 aaaacccatg accggagtga caataccgcc gccaataatg gtcataacga tgaaggacga
    12001 accgtatttg gtgtcctggc cgagattctt aatgcccagc gagaagattg ttgggtactg
    12061 aatcgacata aaggcgctgc ataaagtcag ggctattaag cccacatgac cgccagcgaa
    12121 ggctgagatc aggcacagtg ccatagcgat taatgcgtag gcggccagga ctttgtgtgg
    12181 tgcgaagcga ctgatgagcc aggtaccggt gaaacgacca ataaagaagc acaccatggt
    12241 tccggttaaa tagttagcgg caaagcctgc agtcatacct agaatttctt ctacagcgta
    12301 gcgaatcaaa tagctccagc aggccgtttg tgcgccgaca tagcagaatt gcgctaatac
    12361 cgcccagcgc cagtgacgaa tacgcgccag acgagaaagc gatgcggaga acgatccttg
    12421 tttggcgtca ctgtgattat cactctgcaa tgccgggaat ttcgtcagca tgatcagcag
    12481 ggcgaccagt aacacgatag ccacgatgat catataaggt gtctgtaccg ataataccag
    12541 gctgtgttta tacgcactca attgctctgg agacatttta tcgagaacgt cttgcgattg
    12601 atgtggcacg ttagacaaaa taagactttg cccaaagaca accgcgataa ttgcgccaaa
    12661 cgagttaaat gtttgcgcaa gatttaagcg gaagtgacta ctgctttccg gccctaatac
    12721 cgtaacaaaa gggtttgcgg cagtttccag acaacctaat ccggctgcaa taataaatag
    12781 gccaactaaa aataaggtgt agttcattat ttctgcagcg ggccagaata atgcagcgcc
    12841 caaggcatat aaaaataatc cggtaataat ccctgcttta taactgagtt ttttcatcaa
    12901 tatcccagca gggattggga taatgaaata accaaagtaa aaggccgatt ggatcaggcc
    12961 agcctggaaa tttgtcagcg taaaagcctg ctggaattga ggtaataaaa tgtcgttaag
    13021 gttattggct accgcccaaa gaaaaaacag tgagcacagc agcgcgaatg gaataatgta
    13081 acttctgctt tgccctgcat ctttatctac cgcacggtaa ctctgcgttt gtattgatgt
    13141 gtttcccata gcatcctctt aggttcagaa gcttaagcac gtaatcggtt atgttttcaa
    13201 aagtgcaaaa ccagcgagtt cgggcttcat atcggtgaat atttgcctgt ctgaaatgaa
    13261 ctcatggatt cattttcaaa taaaaaacgc tctgtaatat gacggcggtc acacttatta
    13321 gctggaaggt gactttatgt gactaccatc actttaataa tcgaaatttc atgcccgttt
    13381 cagaaaataa atcataaaaa acacaaacca gatgaccgta ttaattgttt gtgccgaaaa
    13441 cgggcatttc tggcgagggg aaatggaaaa atgaccgttt tgataaatat ttttaagagc
    13501 ggtcattttt aacggggtta taaaaagaga acttgcagtt atttactgtg attagttgag
    13561 ctaggtcaca aaatataagc ttgtgaattt ctttcttgtt aatcaggaag aagatggtgg
    13621 gtaattaaac ggctaattca atagtgtgaa aggaacaaca ttattgccct gttttgaatc
    13681 agagagaggt agctaatgga acgaaataaa cttgctcgtc agattattga cacttgcctg
    13741 gaaatgaccc gcctgggact gaaccagggg acagcgggga acgtcagtgt acgttatcag
    13801 gatgggatgc tgattacgcc tacaggcatt ccatatgaaa aactgacgga gtcgcatatt
    13861 gtctttattg atggcaacgg taaacatgag gaaggaaagc tcccctcaag cgaatggcgt
    13921 ttccatatgg cagcctatca aagcagaccg gatgccaacg cggttgttca caatcatgcc
    13981 gttcattgca cggcagtttc cattcttaac cgatcgatcc ccgctattca ctacatgatt
    14041 gcggcggctg gcggtaattc tattccttgc gcgccttatg cgacctttgg aacacgcgaa
    14101 ctttctgaac atgttgcgct ggctctcaaa aatcgtaagg caactttgtt acaacatcat
    14161 gggcttatcg cttgtgaggt gaatctggaa aaagcgttat ggctggcgca tgaagttgaa
    14221 gtgctggcgc aactttacct gacgaccctg gcgattacgg acccggtgcc agtgctgagc
    14281 gatgaagaga ttgccgtagt gctggagaaa ttcaaaacct atgggttacg gattgaagag
    14341 taatttcgta aagcaacaag gagaaggatg atggctaaca gaatgattct gaacgaaacg
    14401 gcatggtttg gtcggggtgc tgttggggct ttaaccgatg aggtgaaacg ccgtggttat
    14461 cagaaggcgc tgatcgtcac cgataaaacg ctggtgcaat gcggcgtggt ggcgaaagtg
    14521 accgataaga tggatgctgc agggctggca tgggcgattt acgacggcgt agtgcccaac
    14581 ccaacaatta ctgtcgtcaa agaagggctc ggtgtattcc agaatagcgg cgcggattac
    14641 ctgatcgcta ttggtggtgg ttctccacag gatacttgta aagcaattgg cattatcagc
    14701 aacaacccgg agtttgccga tgtgcgtagc ctggaagggc tttccccgac caataaaccc
    14761 agtgtaccga ttctggcaat ccccaccaca gcaggtactg cggcagaagt gaccattaac
    14821 tacgtgatca ctgacgaaga aaaacggcgc aagtttgttt gcgttgatcc gcatgatatc
    14881 ccgcaggtgg cgtttattga tgctgacatg atggatggta tgcctccggc gctgaaagcc
    14941 gcgacgggtg ttgatgcgct cactcatgct attgaggggt atattacccg tggcgcgtgg
    15001 gcactaaccg atgcactgca cattaaagcg attgaaatca ttgctggggc gctgcgagga
    15061 tcggttgctg gtgataagga tgccggagaa gaaatggcgc tcgggcagta tgttgcgggt
    15121 atgggcttct cgaatgttgg gttagggttg gtgcatggta tggcgcatcc actgggcgcg
    15181 ttttataaca ctccacacgg tgttgcgaac gccatcctgt taccgcatgt catgcgctat
    15241 aacgctgact ttaccggtga gaagtaccgc gatatcgcgc gcgttatggg cgtgaaagtg
    15301 gaaggtatga gcctggaagg ggcgcgtaat gccgctgttg aagcggtgtt tgccctcaac
    15361 cgtgatgtcg gtattccgcc acatttgcgt gatgttggtg tacgcaagga agacatttcg
    15421 gcactggctc aggcggcact ggatgatgtt tgtaccggtg gcaacccgcg tgaagcaacg
    15481 cttgaggata ttgtagagct ttaccatacc gcctggtaaa tgcgctgatg tgataatgcc
    15541 ggatacgatt tgcggcgtat ccggctcgcc gttaccgtac caaccgcaat tgctgtaaat
    15601 tgccgtcgat atgcaaatcg gtttgtaagc gggcaatatc gcggcacaga aacgccatct
    15661 ctttatgggt ttctaatttt ttgcgccact tttcggcaac cgcatccaga ttctcatata
    15721 tcccttccag actctgaaac tcgaccagca gctgcgtggc gctttttggt ccgattcccg
    15781 caacacccgg tacctttgaa ctgctgatcc ccgcaagtcc ccagtaatcg ggcaactgct
    15841 gcggttgaac gccaaattct ttatcgataa atggcgcatc cagccaacgt ttctggaagt
    15901 aatcacgaat acgtaatgtc ggtgaaagta actgacagta gcctttatct gtcgaaacaa
    15961 tcgttgcctg atgcccggcc tgtgtcactt tgaccgccag tgtggcggct aagtcatcgg
    16021 cttcgttgcc gctggctgac cagcacggga cgccgcgttg ctcaaaggcg gtgcgtaatg
    16081 caggcatctc gtcgtgcaac tcttccggca ttggcggtcg atccgctttg taatctggta
    16141 aacgctgatg acgccagccg ctactgcggt tttcatcatc aaaaacggcg accgcgtggg
    16201 ttggctggct gtgcataatg agctgatcga gcgcatgctg gcaggtttcg acacagggcg
    16261 aaccctgaac ggcatgaatg cgacggataa gattcagtgc atcgacaata agcaaatgaa
    16321 cagccacgat aaccccctta aagatcaggc gataagggta acattcgatg ccggaacagg
    16381 ctatggctgg ttgagaaaac aggaagaggc ttcgcaaaac gaggcctctg ggagagatta
    16441 atcgcaggca acgatcttca tcgccaggcc gccgcgagag gtttcgcggt acttggcgtt
    16501 catgtcttta cctgtttcgt acatggtttc gataacttta tcgaggcaga cgcgcggttc
    16561 gctggtacgg cgcagtgcca tacgcgcggc gttcaccgct ttcactgctg caatggcgtt
    16621 acgctcaatg cacggtacct gaacctgccc cgcgaccggg tcacaagtca gaccgaggtt
    16681 gtgctccatc gcgatttccg ctgcgatgca aacctgcgcc gggcttgcgc ctaacagttc
    16741 tgccagacca gctgccgcca tagagcacgc cacgccaact tcaccctggc aacccacttc
    16801 ggcaccagaa atcgacgcgt tcatcttata aagagaacca atggcgctgg ctaccagcag
    16861 gtaacgagcc agtgagttag cgttcacttc gcggataaac ttatcgtagt acgccagcac
    16921 agccgggata atcccgcacg caccgttagt cggcgcagtc accacgcgac cgccagcagc
    16981 gttctcttcg ttcactgcca gtgcaaacat gttgatccag tcaacaaccg ccatcgggtc
    17041 agtggtggtt ttatcctggc tgaccagcat ccggcgtagt gccgcagcac ggcgtggaac
    17101 gcgcagtttg ccaggcaaca cgccttcggt ggaaataccg cgctcaatac cgccacgcat
    17161 gacttcccag acgttcgcca ggtgctgttc cagctcttct ttactgtgca gcgccagctc
    17221 gtttttcatc atcaggccag agagtgacag cccggtttcc tgacaatgtt tttgcagatc
    17281 ggctgctgaa ctgtacggat aaggaacttc aaccggtgcg ctatcctgct ggccaaaatg
    17341 ctcttcatca acgataaagc caccgccaat agagtagtaa gtctggctgt aaacgacttt
    17401 atcgcccgcc agcgcggtaa tgcgcatacc gttttcatgc agagaaaggt tgtctgcatg
    17461 gaagttcatg cactgatcaa ccgggaactc cacttcatgc tgaccgtttg ccagcatcag
    17521 gcgaccatga gtattcacat cctgaataaa accggggatg gaatcgatat ccacggtatc
    17581 cggcaggtta cccgccaggc ccataataat ggcgatatct gtgtggtggc ctttaccagt
    17641 cagagagagc gagccgtaca cgtcaaccac cacgcgggtc acgtctttaa gcaggttacg
    17701 ggcaatcaga tcgtcggtaa attgtttacc cgctttcatt ggtccaacgg tatgagaact
    17761 ggaagggcca atgccgattt tgaaaatatc gaatacgcta atcataggaa atacatcgcg
    17821 ttaaaacgga ggaagcgccg cccgaaagcg gcgcgaaagg acttagctga acagagagta
    17881 gaagattgcg gagattgcaa tcagacccat cacgacaacg aatacgttgc tgatgtgacc
    17941 gctgtactta cgcattgccg gtactttctg aattgcgtac atcggcatca ggaacaggat
    18001 catcgcgatg attgggccgc ccagggtttc aatcataccc aggatgctcg ggttcagggt
    18061 ggcaacaatc caggtcgtta ccagcatgaa cagcgcagtg atacggttca gcttgttgat
    18121 ttcgatagac ttacctttac cacgcagaga tttaatcacc ataccgttga agccttcacg
    18181 tgcgcccagg tagtggccga ggaaggattt ggtgatagcg ataatcgcga taatcggagc
    18241 catccacgcg ataaccggtg cgttaaagtg gttagccagg taagacagaa tcgagatgtt
    18301 ctgctcttta gccgcagcca ggtctgccgg agtcaggctc agtacgcagc tgaagacgaa
    18361 gaacattacg gtcagcacca tcatgatgtg tgcgaatgcc aggatcttcg agcatttctg
    18421 ttctgccata tcgccgtact cttcacgctt cgcaacggcg aaagaagaga tgatcggaga
    18481 gtggttgaac gagaacacca ttaccggaat tgccagccac agggtcatcc acagaccgtt
    18541 tccggttgca gatgcagtgt ccagagacag cgtttccagt gctgcgccgt tccactgcgg
    18601 gatcaggtac agagccagca gcatcagtac gccaacaaac gggaatacca gaatactcat
    18661 cgctttaacg atcatctgct caccaaagcg aacgatggtc atcataccca cgatcaggat
    18721 cagcgacaga atcgcacgcg gcggtggcgt catacccagc tggtgagaca tgaagctttc
    18781 aacggtattg gtgattgcca cgctataaac cagcaggatc gggtagatag cgaagaagta
    18841 gagcagggta atcagtttac ctgcgccaat accaaagtgt tcttctacaa cctcggtgat
    18901 gtcttcgccc gggtttttac cagacagtac gaagcgagtc aggccgcggt gagcaaaaaa
    18961 cgtcatcggg aacgcaagga tagccatgat gatcagcggg atcataccgc caacaccggc
    19021 gttgattggc aggaacagca cgcccgcgcc gattgccgtg ccgtaaaggc ccagcatcca
    19081 catggtgtct gtcttgcgcc aggcactacg agagtctttc gacgcaatcg tgctggtttg
    19141 agtcgtttcc atctatttct cctggaggaa tgcaaataat tcagctttta gccagttcag
    19201 cccgggaagg tgcctgaccg taaaaatgaa atctgtctat cggcgatggc ttaatcattt
    19261 accgccgtaa taatttgagg gcggaaagat acacacaagt tatgtatcta tcagtgatct
    19321 tgatcccaaa tacaataatc tgcttggaag atagattatc ccaacgcttt actctgtaaa
    19381 tcaaaagtaa aagagaattt aagggcaata tttgcccatt aaacgacatc tgacttcttc
    19441 cggggcgcgc aatatatgta ggttgaactg aaaaaatcaa tacttttagt taatcatggc
    19501 gataatttct ttttcatagc gataaaatac ggtttatgaa aatagggaaa cgtttgtgtt
    19561 gcaacataaa ttgtatgaca tattaactgt gatgatattt gtcgcgtgag ggtggtaaga
    19621 aacattcata taacaaatga aatgagttga tgagtaagtg accaaaaacg agaaaacccg
    19681 gcaa
//
LOCUS       DADLTY010000077        19501 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.077, whole genome shotgun sequence.
ACCESSION   DADLTY010000077 DADLTY010000000
VERSION     DADLTY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.077"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            389..2047
                     /locus_tag="JN334_002252"
     CDS             389..2047
                     /locus_tag="JN334_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAY7438381.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="JN334_002253"
     CDS             complement(2044..3000)
                     /locus_tag="JN334_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAY7438382.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="JN334_002254"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="JN334_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HAY7438383.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="JN334_002255"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="JN334_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HAY7438384.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="JN334_002256"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="JN334_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HAY7438385.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="JN334_002257"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="JN334_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAY7438386.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="JN334_002258"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="JN334_002258"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAY7438387.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="JN334_002259"
     tRNA            complement(9859..9946)
                     /locus_tag="JN334_002259"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="JN334_002260"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="JN334_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAY7438388.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="JN334_002261"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="JN334_002261"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAY7438389.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="JN334_002262"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="JN334_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HAY7438390.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="JN334_002263"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="JN334_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HAY7438391.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="JN334_002264"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="JN334_002264"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAY7438392.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="JN334_002265"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="JN334_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HAY7438393.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..>19501
                     /gene="ftsK"
                     /locus_tag="JN334_002266"
     CDS             17198..>19501
                     /gene="ftsK"
                     /locus_tag="JN334_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY7438394.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQP"
BASE COUNT         4895 a         4805 c         5222 g         4579 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgt cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcaggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagtgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacagtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagcagcagt atcagcagcc
    19501 a
//
LOCUS       DADLTY010000078        19307 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.078, whole genome shotgun sequence.
ACCESSION   DADLTY010000078 DADLTY010000000
VERSION     DADLTY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.078"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..2095
                     /gene="pbpC"
                     /locus_tag="JN334_002267"
     CDS             <1..2095
                     /gene="pbpC"
                     /locus_tag="JN334_002267"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708358.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HAY7438395.1"
                     /translation="ARYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGS
                     TLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGA
                     ASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWS
                     REQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNW
                     KGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVY
                     GLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEA
                     YGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLR
                     LQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWA
                     IGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVT
                     RGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQ
                     RVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGV
                     RDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLV
                     MDDVGQIATVKFVMQ"
     gene            2245..2676
                     /gene="ndk"
                     /locus_tag="JN334_002268"
     CDS             2245..2676
                     /gene="ndk"
                     /locus_tag="JN334_002268"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAY7438396.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2826..3980
                     /gene="trmG/rlmN"
                     /locus_tag="JN334_002269"
     CDS             2826..3980
                     /gene="trmG/rlmN"
                     /locus_tag="JN334_002269"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAY7438397.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4265..5278
                     /gene="rodZ"
                     /locus_tag="JN334_002270"
     CDS             4265..5278
                     /gene="rodZ"
                     /locus_tag="JN334_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HAY7438398.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5305..6432
                     /gene="ispG"
                     /locus_tag="JN334_002271"
                     /gene_synonym="gcpE"
     CDS             5305..6432
                     /gene="ispG"
                     /locus_tag="JN334_002271"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAY7438399.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6535..7809
                     /gene="hisS"
                     /locus_tag="JN334_002272"
     CDS             6535..7809
                     /gene="hisS"
                     /locus_tag="JN334_002272"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAY7438400.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7827..8447
                     /locus_tag="JN334_002273"
     CDS             7827..8447
                     /locus_tag="JN334_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HAY7438401.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8458..9636
                     /gene="bamB"
                     /locus_tag="JN334_002274"
     CDS             8458..9636
                     /gene="bamB"
                     /locus_tag="JN334_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAY7438402.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9754..11226
                     /gene="der"
                     /locus_tag="JN334_002275"
     CDS             9754..11226
                     /gene="der"
                     /locus_tag="JN334_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAY7438403.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11296..11511
                     /locus_tag="JN334_002276"
     CDS             11296..11511
                     /locus_tag="JN334_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438404.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="JN334_002277"
     CDS             complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="JN334_002277"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAY7438405.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="JN334_002278"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="JN334_002278"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAY7438406.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="JN334_002279"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="JN334_002279"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAY7438407.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16247..16786)
                     /locus_tag="JN334_002280"
     CDS             complement(16247..16786)
                     /locus_tag="JN334_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438408.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16802..17320)
                     /locus_tag="JN334_002281"
     CDS             complement(16802..17320)
                     /locus_tag="JN334_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY7438409.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17631..17822)
                     /locus_tag="JN334_002282"
     CDS             complement(17631..17822)
                     /locus_tag="JN334_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HAY7438410.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17840..17992
                     /locus_tag="JN334_002283"
     CDS             17840..17992
                     /locus_tag="JN334_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438411.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18174..>19307
                     /gene="pdeF"
                     /locus_tag="JN334_002284"
     CDS             18174..>19307
                     /gene="pdeF"
                     /locus_tag="JN334_002284"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY7438412.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4545 a         4701 c         5250 g         4811 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc
      481 ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
      541 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
      601 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
      661 gcggttgcca ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt
      721 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
      781 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct
      841 ggatgaaggc ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga
      901 ttatcgacca ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct
      961 ggtgcgctcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     1021 tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa
     1081 tctttcactc attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac
     1141 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     1201 tgaacggcct ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga
     1261 agcgcaaccc ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     1321 caccagctat ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg
     1381 gatctggact ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     1441 cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga
     1501 agacccgcgc ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt
     1561 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca
     1621 gccgccaacg ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg
     1681 gctggatgac aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt
     1741 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     1801 cttaccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca
     1861 gctgacaggc gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     1921 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc
     1981 gttaactgaa cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt
     2041 gctggtgatg gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt
     2101 tttgtccctg attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat
     2161 agtcaacggc cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca
     2221 acagaacaat ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg
     2281 aacgcggtag caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc
     2341 aaaattgtcg gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct
     2401 gaacacgatg gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc
     2461 gtggtttccg tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg
     2521 accaatccgg caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc
     2581 gaaaacggta cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc
     2641 ttcggcgaag gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa
     2701 acgtggcatc cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca
     2761 ccggggcgtt tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat
     2821 agcatatgtc tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca
     2881 acctgctgga tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa
     2941 ccttccgcgc cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg
     3001 agatgaccga catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg
     3061 caccggaagt ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg
     3121 ttggcgatca gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg
     3181 tctcttcgca ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct
     3241 ttaaccgcaa cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg
     3301 tcggcgcagc aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg
     3361 gcgagccgct gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt
     3421 tcggttttgg cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc
     3481 tggataaact gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg
     3541 acgaaattcg tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg
     3601 ctgcggtgcg ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat
     3661 acgtgatgct tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc
     3721 tgaaagatac gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt
     3781 atggacgcag ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt
     3841 tcaccactat tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg
     3901 cgggcgatgt tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca
     3961 tcgatattaa agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa
     4021 tcttttatga atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg
     4081 ctgactttaa ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt
     4141 tgtcacgagc acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc
     4201 gcggtgtggg tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca
     4261 gcgaatgaat actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct
     4321 gcgtaatgct cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct
     4381 gaaggtttcc acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac
     4441 attcctgcgc ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact
     4501 gctgccaggg ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag
     4561 tttttccctc ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct
     4621 ggtgttgttt gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca
     4681 gcaggaagag atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga
     4741 gcaggggcag agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac
     4801 gccgccagcg tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc
     4861 agcaccagct gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga
     4921 taccgccgcg accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc
     4981 aaccgatcag gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt
     5041 tactgccgat tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat
     5101 gcagcgtaaa gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg
     5161 tgcgccagcc gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat
     5221 cagaactaac caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca
     5281 gacgggtaac gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca
     5341 acacgtattt acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc
     5401 atgaccaata cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa
     5461 cgcgttggcg ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc
     5521 aaactcatca aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc
     5581 attgcgctga aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc
     5641 ggtaatgaag agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc
     5701 cgtattggcg ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg
     5761 acgccgcagg cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac
     5821 ttcgatcagt tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat
     5881 cgtttgctgg caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt
     5941 gcacgcagcg gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc
     6001 ggcgacacgc tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc
     6061 gatattttga aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc
     6121 tgttcgcgtc aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa
     6181 gatatcatca ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag
     6241 gcgctggttt ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat
     6301 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
     6361 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa
     6421 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg
     6481 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
     6541 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
     6601 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
     6661 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
     6721 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
     6781 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
     6841 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
     6901 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
     6961 gaactgatta tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact
     7021 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
     7081 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
     7141 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
     7201 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
     7261 ctgctggaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
     7321 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
     7381 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
     7441 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
     7501 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
     7561 gcggctatgg cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc
     7621 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
     7681 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
     7741 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
     7801 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
     7861 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg
     7921 gcgttggcgc actgactggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
     7981 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
     8041 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
     8101 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
     8161 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
     8221 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
     8281 aaggtgaagg gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag
     8341 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
     8401 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
     8461 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
     8521 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
     8581 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
     8641 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
     8701 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
     8761 ggctggttct ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat
     8821 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
     8881 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
     8941 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc
     9001 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
     9061 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
     9121 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
     9181 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
     9241 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
     9301 gaactgggtt cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa
     9361 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat
     9421 ctgctgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac
     9481 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
     9541 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
     9601 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
     9661 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
     9721 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
     9781 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
     9841 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
     9901 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
     9961 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    10021 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    10081 gaaaaaccga ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg
    10141 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    10201 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    10261 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    10321 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    10381 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt
    10441 gtttacgaca tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga
    10501 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    10561 gtagagaaat tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg
    10621 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    10681 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    10741 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    10801 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    10861 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    10921 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    10981 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    11041 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    11101 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    11161 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    11221 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct
    11281 accggaggaa ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat
    11341 aacggccatg cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca
    11401 gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag
    11461 cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt
    11521 ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc
    11581 agacgtgtgg ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg
    11641 tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt
    11761 tctgctaaac gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt
    11821 ggattttgct gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt
    11941 gccagccgga gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt
    12001 gtacggttgg cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc
    12061 tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc
    12121 gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg
    12181 acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat
    12241 aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga
    12301 ttcgccagtt caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg
    12361 gggtagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt
    12421 agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt
    12481 tttttgtatt gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac
    12541 ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg
    12601 ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga
    12661 cggaaggtga cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg
    12721 tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat
    13441 caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac
    13501 gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt
    13741 tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc
    13801 agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc
    13921 ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg
    13981 tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc
    14041 ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg
    14101 cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 tttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt
    14761 actactgaag aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt
    14941 cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc
    15601 gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc
    15661 ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaacg gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt
    16141 gagtgggaat gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag
    16201 ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc
    16261 tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag
    16321 tcatatttct gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct
    16381 tcagttcctg atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg
    16441 ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt
    16501 ttttgtccgc agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg
    16561 ccgttgccgc tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct
    16621 ggatttccct gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac
    16681 cttgctgttc cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg
    16741 gatagcttaa ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt
    16801 attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg
    16861 taataacaac ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat
    16921 acactttggt gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca
    16981 ccatagaacc cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg
    17041 aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg
    17101 cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa
    17161 gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat
    17221 aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta
    17281 acattgccac aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt
    17341 acgaaaaata agacatttaa gatcatcaaa agaggaaagt aaatgtattg cgtataaagg
    17401 gatagattaa atttattaaa attcacgcag ggaataattt ataaaaatat atcattacaa
    17461 catatatatt aattttatat ttatttcgca gccaaacagg ataaacatca gtcaggacaa
    17521 agacgtaaaa tttaagatga aagaatatct gccgctttga taatcccaaa gagcaaaagc
    17581 atccgacagc gaagtgccgt tatgttctgt gaaagtggag aaggtaaccg ctaacgttgt
    17641 accggtgatt cgacggagag tgtggattgc tgagcttctt ttttgctctg atggtgctgc
    17701 caggcaccga cggacgagta gataaaacgg ccaaagaaga agataaagct gatgagcagt
    17761 acgatacggg tcatgcgact gttaaatcgg tgtcgttttc gcatactagt tgcctgactc
    17821 acaaaaggtt ccttgaagta tgtcccacgc tgtggacggt acttacatta aggcacaaca
    17881 ggacaaaatg gtcaattctt cgttatgtaa aaaagcgtca gtggatacat attttaatgt
    17941 tatggaagtt aatttaatta tttacaatga tgatgtaata atgatgaatt aacatgataa
    18001 acgtaataat tcattaatca gggttatttt gtttgatata tatcaattgg aactttcata
    18061 tgacacttag aatcaatgct ctctatagtg atgaataatc atcattcgaa gtcaggtggg
    18121 atgcctgtct gaatacacct ccttcaggat gtgggggatt ctgctgagca tctatgaaac
    18181 tgaatgcaac ttatataaaa atacgtgata aatggtgggg gcttccgctg ttcctgcctt
    18241 ctttaatctt gcccattttc gcccacatta atactttcgc gcatatttct tccggtgagg
    18301 tttttctctt ttatctgcct ttggcactga tgatcagcat gatgatgttt ttcagctggg
    18361 cggcattgcc agggatcacc ttagggattt ttgtccgcaa atatgcagag ctgggttttt
    18421 acgaaacgct ctcattaacg gctaatttta ttatcattat cattctctgt tggggcggtt
    18481 acagggtctt tactccccgg cgtaacaacg tttcacatgg tgatacccgt ttaatttccc
    18541 agcgtatatt ctggcagata gtgtttcctg caacgctgtt tctgatactt ttccagtttg
    18601 ctgcatttgt aggattactg gcgagcagag aaaatctggt cggtgtcatg ccttttaacc
    18661 tcgggacttt aatcaattat caggccttac tggtgggtaa tctgatcggt gtcccgctgt
    18721 gctacttcat cattcgggta gtgcgaaatc cattttattt acgtagctat tattcgcaat
    18781 taaaacagca ggttgatgcc aaagtcacca aaaaagagtt cgcgctctgg ctactggcat
    18841 taggtgcttt attgttgctg ttatgcatgc cgttaaatga aaaaagcaca atttttagca
    18901 ccaattatac cttgtcatta ttgctgcccc tgatgatgtg gggagcaatg cgctatggtt
    18961 ataagctgat ttcgctgctc tgggcggtcg tgttgatgat cagcatccac agctatcaaa
    19021 attacattcc catttatcct ggctatacca cgcagctgac cataacctcc tccagttatc
    19081 tggtattctc ttttattgtc aattatatgg ctgtactggc aactcgtcag cgagcggtag
    19141 tcagacgcat tcagcggctt gcgtatgtgg acccggtggt tcatctgcca aatgttcgcg
    19201 ccctgaatcg cgcgttgcgt gatgccccct ggtctgcgct ttgttattta cgcatccctg
    19261 gcatggaaat gctggttaag aactatggca tcatgctgcg gattcaa
//
LOCUS       DADLTY010000079        18701 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.079, whole genome shotgun sequence.
ACCESSION   DADLTY010000079 DADLTY010000000
VERSION     DADLTY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18701
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.079"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="JN334_002285"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="JN334_002285"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAY7438413.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="JN334_002286"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="JN334_002286"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAY7438414.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="JN334_002287"
     CDS             complement(3121..3420)
                     /locus_tag="JN334_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438415.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /locus_tag="JN334_002288"
     CDS             3595..4590
                     /locus_tag="JN334_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7438416.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="JN334_002289"
     CDS             complement(4632..5069)
                     /locus_tag="JN334_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HAY7438417.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="JN334_002290"
     CDS             complement(5115..5456)
                     /locus_tag="JN334_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438418.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="JN334_002291"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="JN334_002291"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAY7438419.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="JN334_002292"
                     /pseudo
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="JN334_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="JN334_002293"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="JN334_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7438420.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="JN334_002294"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="JN334_002294"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HAY7438421.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="JN334_002295"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="JN334_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HAY7438422.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13865
                     /locus_tag="JN334_002296"
                     /pseudo
     CDS             12687..13865
                     /locus_tag="JN334_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
     gene            complement(13973..14797)
                     /locus_tag="JN334_002297"
     CDS             complement(13973..14797)
                     /locus_tag="JN334_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HAY7438423.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="JN334_002298"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="JN334_002298"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY7438424.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="JN334_002299"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="JN334_002299"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HAY7438425.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
BASE COUNT         4719 a         4736 c         4585 g         4661 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaaccct tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 caaagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga
     7801 agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaaatggg taattcctcg
     8641 aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggtgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt
     9181 atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc
     9661 gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat
     9721 aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gccttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg
    10021 aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgagcg agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcacccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata
    11221 ttggcgcgaa atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct
    12061 ccggcctcta ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgcccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggtg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg acaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga cgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacacctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggtaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt c
//
LOCUS       DADLTY010000080        18086 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.080, whole genome shotgun sequence.
ACCESSION   DADLTY010000080 DADLTY010000000
VERSION     DADLTY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.080"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            256..780
                     /locus_tag="JN334_002300"
     CDS             256..780
                     /locus_tag="JN334_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7438426.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            861..1535
                     /locus_tag="JN334_002301"
     CDS             861..1535
                     /locus_tag="JN334_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7438427.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1551..4031
                     /locus_tag="JN334_002302"
     CDS             1551..4031
                     /locus_tag="JN334_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7438428.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            4047..5080
                     /locus_tag="JN334_002303"
                     /pseudo
     CDS             4047..5080
                     /locus_tag="JN334_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5162..5500)
                     /gene="rcnB"
                     /locus_tag="JN334_002304"
     CDS             complement(5162..5500)
                     /gene="rcnB"
                     /locus_tag="JN334_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HAY7438429.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5719..6549)
                     /locus_tag="JN334_002305"
                     /pseudo
     CDS             complement(5719..6549)
                     /locus_tag="JN334_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6671..6943
                     /gene="rcnR"
                     /locus_tag="JN334_002306"
     CDS             6671..6943
                     /gene="rcnR"
                     /locus_tag="JN334_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HAY7438430.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      7035..7131
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7166..7954
                     /gene="thiM"
                     /locus_tag="JN334_002307"
     CDS             7166..7954
                     /gene="thiM"
                     /locus_tag="JN334_002307"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HAY7438431.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7951..8751
                     /gene="thiD"
                     /locus_tag="JN334_002308"
     CDS             7951..8751
                     /gene="thiD"
                     /locus_tag="JN334_002308"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAY7438432.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8816..9637
                     /locus_tag="JN334_002309"
     CDS             8816..9637
                     /locus_tag="JN334_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HAY7438433.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9685..10431
                     /locus_tag="JN334_002310"
     CDS             9685..10431
                     /locus_tag="JN334_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7438434.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10405..11370)
                     /locus_tag="JN334_002311"
     CDS             complement(10405..11370)
                     /locus_tag="JN334_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAY7438435.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11367..12370)
                     /locus_tag="JN334_002312"
                     /pseudo
     CDS             complement(11367..12370)
                     /locus_tag="JN334_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12410..13462
                     /gene="fbaB"
                     /locus_tag="JN334_002313"
     CDS             12410..13462
                     /gene="fbaB"
                     /locus_tag="JN334_002313"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HAY7438436.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13770..14624
                     /gene="gatY"
                     /locus_tag="JN334_002314"
     CDS             13770..14624
                     /gene="gatY"
                     /locus_tag="JN334_002314"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HAY7438437.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14653..15915
                     /gene="gatZ"
                     /locus_tag="JN334_002315"
     CDS             14653..15915
                     /gene="gatZ"
                     /locus_tag="JN334_002315"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HAY7438438.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15925..16377
                     /gene="gatA"
                     /locus_tag="JN334_002316"
     CDS             15925..16377
                     /gene="gatA"
                     /locus_tag="JN334_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HAY7438439.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16408..16692
                     /gene="gatB"
                     /locus_tag="JN334_002317"
     CDS             16408..16692
                     /gene="gatB"
                     /locus_tag="JN334_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAY7438440.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16696..18051
                     /locus_tag="JN334_002318"
     CDS             16696..18051
                     /locus_tag="JN334_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAY7438441.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4731 a         4115 c         4576 g         4664 t
ORIGIN      
        1 aatcatttat ataaatgatt tcgtcttttt tattgatatc aacaatacca tttacataaa
       61 tctacattaa ttaatagatg tgataaatat ttaatattct taagtatttg ttgtatttat
      121 attgcattgg ttaatttgtt ttaaatcaat aaaacaatat gtttacattg cggcagcttt
      181 tttgatgtga gaaatttgtt tccaagagat acagactctt aacaaccaaa aataaatttt
      241 attaaggaac atattatgaa acgttcaatt attgctgccg ctgtcttttc ttcttttttt
      301 atgagcgctg gagtatttgc tgcagacgtt gataccggaa cattaaccat taaaggaaat
      361 attgcagaat ctccgtgtaa attcgaagcg ggtggtgatt cagtaagtat taatatgccg
      421 actgtaccaa ccactgtctt tgaaggtaaa gctaaatatt ctacctatga tgatgcagtc
      481 ggtgtaacca gcagcatgtt aaaaattagc tgcccgaaag aagttgctgg tgtaaaactc
      541 tcgttgatca caaacgacaa aataaccggt aacgataagg cgatagccag tagcaacgat
      601 accgtaggtg ataacagcga tgtcctagat gtttctgcac cttttaacat tgagagttat
      661 aaaacagcgg aaggtcaata tgctattccg tttaaagcaa aatacctgaa actgacagat
      721 aactcagtgc aatcaggtga tgtgttatct tctctggtta tgcgtgtggc gcaggattaa
      781 taaataaaat tgcaagtttc tccaggtaat aaacatggcc gctatcccca tggcggcttt
      841 ttaatttaag aggcattaag atgaaaggat tattatcttt actcattttt tctatggtcc
      901 ttcctgcaca tgccggaatt gttatctacg ggacgcgcat tatttacccg gcagaaaata
      961 aagaagttat ggtgcagttg atgaaccagg gaaatcgttc ttcgctgctg caggcgtgga
     1021 ttgatgatgg cgatacgtcg ttaccaccag aaaaaattca ggttcctttc atgttaacgc
     1081 caccagtggc aaaaataggg gcaaattccg ggcagcaagt aaaaatcaaa attatgccga
     1141 ataaactgcc cactaataaa gaaagcattt tttatctgaa tgttctgtat attccaccaa
     1201 atagtccaga gcaagaaggc aagaatgcac tgaagtttgc gatgcaaaac agaattaagt
     1261 tgttttaccg gccagcgggt attgctccgg taaataaagc gacctttaaa aagttactgg
     1321 taaatcgcag tggcaatgga ttggtgataa aaaatgactc agctaattgg gtgacgattt
     1381 cggatgtcaa agctaataat gttaaagtca attatgaaac tattatgatt gcccccttag
     1441 aaagtcagag tgttaatatc aaaagtaata atgcaaataa ctggtatctg accattatca
     1501 atgaccacgg caactatatt agtgacaaaa tttaattgca ggagctgcct atgttgagaa
     1561 tgaccccact tgcatcagca atcgtagcgt tattgctcgg cattgaagct tatgcagctg
     1621 aagaaacctt tgatacccat tttatgatag gtggaatgaa agaccagcag gttgcaaata
     1681 ttcgtcttga tgataatcaa cccttaccgg ggcagtatga catcgatatt tatgtcaata
     1741 agcaatggcg cgggaaatat gagattattg ttaaagacaa cccgcaagaa acatgtttat
     1801 caagagaagt tatcaagcgg ttaggcatta atagcgataa cttcgccagc ggtaagcaat
     1861 gtttaacatt tgagcaactt gttcagggtg ggagctatag ctgggatatc ggggtttttc
     1921 gtctcgattt cagtgtcccg caggcttggg tggaagaact ggaaagtggc tatgttccac
     1981 cggaaaactg ggagcggggt attaatgcgt tttatacttc ttattatgtg agtcagtatt
     2041 acagcgacta taaagcgtcg ggtaacaaca agagtacata tgtacgtttt aacagcgggt
     2101 taaatttact ggagtggcaa ctgcattctg atgccagttt cagtaaaaca aataacaatc
     2161 caggggtgtg gaaaagcaat accctgtatc tggaacgtgg atttgcccaa tttctcggca
     2221 cgcttcgcgt gggtgatatg tacacatcaa gcgatatttt tgattctgtt cgcttcagcg
     2281 gtgtgcggtt gtttcgtgat atgcagatgt tgcctaactc gaaacaaaat tttacgccac
     2341 gggtgcaggg gattgctcag agtaacgcgc tggtaactat tgaacagaat ggttttgtgg
     2401 tttatcagaa agaggttcct cctggcccgt ttgcgattac agatttgcag ttggccggtg
     2461 gtggagcaga tcttgatgtt agcgtgaaag aggcggacgg ctcggtaacc acctatctgg
     2521 tgccttatgc agcggtgcca aatatgctgc aacccggcgt gtcgaaatat gattttgcgg
     2581 cgggccgtag ccatattgaa ggggcgagca agcaaagtga ttttgtccag gcaggttatc
     2641 agtatggttt taataactta ttgacgctgt atggcggcac gatggttgct aataattact
     2701 atgcgtttac cctcggaacg ggttggaaca cgcgcattgg cgcaatttcc gtcgatgcca
     2761 cgaagtcgca tagtaaacaa gacaacggcg atgtgtttga cgggcaaagt tatcaaattg
     2821 cctacaacaa atttgtgagc caaacgtcga cgcgttttgg tctggcggcc tggcgttatt
     2881 cgtcgcgtga ttaccggaca tttaacgatc acgtttgggc aaacaataaa gataattatc
     2941 gccgtgatga aaacgatatc tatgacattg ccgattatta ccagaacgat tttggccgta
     3001 aaaatagctt ttctgccaat atgagccagt cattgccaga aggctggggt tctgtgtcct
     3061 taagtacgtt atggcgagat tactgggggc gtagcggcag cagtaaggat tatcagttga
     3121 gttattccaa taactggcgg cggataagct ataccctcgc ggcaagccag gcgtatggcg
     3181 agaatcatca tgaagagaaa cgttttaata tttttatatc aattccctgt gattggggtg
     3241 atgacgttac gacgcctcgt cggcaaatat atatgtctaa ctcaacgacg tttgatgatc
     3301 agggttttgc ctcaaataat acgggattat caggaacagt agggagtcgg gatcagttca
     3361 attatggtgt caacctgagt catcaacatc agggaaatga aacgacagct ggggcgaatt
     3421 tgacctggaa cgcgccggtt gcgacagtga atggcagtta tagtcagtcg agtacttatc
     3481 gacagactgg agccagtgtt tcagggggca ttgtcgcctg gtcgggtggc gttaatctgg
     3541 cgaaccgtct ttccgaaacg tttgctgtga tgaatgcgcc aggaattaaa gatgcttatg
     3601 tcaatgggca aaaatatcgc acaacaaacc gtaatggagt ggtggtatac gacggaatga
     3661 caccttatcg ggaaaatcac ctgatgctgg atgtgtcgca aagcgatagc gaagcagaat
     3721 tacgtggcaa ccggaaaatt gccgcccctt atcgcggcgc ggttgtactg gttaattttg
     3781 ataccgatca gcgcaagccc tggtttataa aagcgttaag agcggatggt caaccattaa
     3841 cgtttggtta tgaagtcaat gatatccatg gtcataatat tggcgttgtc ggccagggaa
     3901 gccagttatt tattcgcacc aatgaaatac cgccatcggt taatgtagca attgataagc
     3961 aacaaggact ttcatgcaca atcaccttcg gtaaagagat tgatgaaagt agaaattata
     4021 tttgccagta atagtcaggt ggttctatgg aaattcgcat aatgctattt atattaatga
     4081 tgatggttat gcctgtgagc tatgcggcat gttatagtga gttatctgtt cagcacaatt
     4141 tggttgttca ggggaatttt gcacttactc aaacacaaat agcgacatat gagcataatt
     4201 ttaatgattc gtcatgcgtg agtacaaata ctatcacccc tatgagccca tcggatatta
     4261 ttgttggact ttataacgat accattaaat taaatttaca ttttgaatgg accaataaaa
     4321 acaacatcac gttgtcaaat aatcagagca gtttcaccag tggttattcg gtaacggtga
     4381 cacctgcggc cagtaatgca aaagtgaata tttctgcggg gggcggtggt tcagtgatga
     4441 ttaatggtgt tgcgacatta tccagtgctt catcatcgac acgcgggagt gccgcagtac
     4501 aatttctact gtgtttatta ggtggcaagt catggggtgc atgtgtaaat agctacagaa
     4561 atgcattggc acaaaatgca ggtgtctatt ccttcaatct gacattgtca tacaacccga
     4621 taaccacaac ctgcaaacag gacgatttat taattacttt agacagtatt cccgttttac
     4681 aattaccagc cacaggtaac aaagcaacaa taaatagtaa aaaaagggat attattctgc
     4741 gttgtaaaaa tttattaggt caacaaaatc aaacatcacg gaaaatgcag gtgtatttat
     4801 caagttctga cttgttaacc aacagcaaca caatactgaa aggtgcggaa gataatgcgt
     4861 aggatttatt cttgaaagta atggttcgcc agtcacactt ttaaatatca ctaacagcag
     4921 taaaggatac acaaatttaa aagaaattgc ggcaaagtca aaacttacag atacaacggt
     4981 ttcaattccg ataacagcca gttactacgt ctacgataca aacaaagtta aatctggcgc
     5041 actggaggca accgcattaa tcaacgtgaa atacgactaa taacaagcaa aacgagcaag
     5101 tggctaataa taaaaaataa caaggtgtct gcatttcccc ctcatgatga gggggctttt
     5161 tttagcgatg ataaaaaatc tcaccgtcgt aggctttaat gattttaccg tcggtgtcgc
     5221 tgatcaacac gtacgcgcca cccatatagg tccagtgcgt cccggcatca ggcgcgggca
     5281 ggttacgtaa ctgccactgc ttaatgttgt actcatcggt gcggtacatc tctgggacgg
     5341 tatcacctgg tttgaaatgc ttaaagtcag caatgaaact acttaactca tacttagaaa
     5401 ttcccgaggt attggtcgaa cccgcggtgg ctggtgcggc ccaggcggca ctggtaacca
     5461 gcaaaagcac acccagcaac atcttatttt taatggtcat aaactctcca ctttggggat
     5521 ctgtaatatc agtcgcatat ctttcctgtc atacaatata actgacaagc gatattttgt
     5581 tagtgatccc atgatttcgt aaaaagtgta aatgaaagga tgccctaaaa gcatccttag
     5641 cgagcagaga gtcagcggaa atagctgatt taagaatcat cctgggctga agataacatg
     5701 caagcctaaa ggattgagtt atcgcattat gcccatgaag ccatgtacgc ccatatacac
     5761 accgactaag ccaatcaaca gactggaaaa atagggggcg cgtttagcga gagtgttaaa
     5821 tccgctccag cgttttgcga cctgctgaac gctgattgct gcgccaacgc ctacggtgac
     5881 aagcgttaac gccaggccaa tgctgaaact gacgaccagt gttgcgccca gtgtcagggc
     5941 tttcaactga atgcatatca acagcacggt aattgctgcc gggcagggga taaagccacc
     6001 ggttaagcca aataacaaaa tttgccagtt gatgacctct ctaccatcaa agcgtcgttt
     6061 aatgtcattg gcatgggctc gtgcatgggc atcctgatac tcgccatgtt cgtgatggtg
     6121 atgatgtcct tggtcgtggt gatgttcgtg atcgtgatga tggtgatgat gctcataatc
     6181 atgcccgtgc atattctcca gccagttgcg ttcgccgcgc taggtacgcc agaacatcca
     6241 gaacgcggtg ccaataatga tcactgcgga aatcagctgg agccacggtt ctgctgattg
     6301 agcagtaaag cgcttactga tcaccatccc gccaaaggca atcaaccaga ccaccgcggt
     6361 atgcgaaatg gttgctgcca gtccgagcat caccgcctgt ttaatggtgc ctttgatggc
     6421 gatgataaac gccgccatca tcgtttttga gtgccctggt tccaggccat gaagcgcacc
     6481 aagtaagatg gcgctgggga tgaagaacca ggcgtttcct tgctgaagaa gagttgtaaa
     6541 ttcggtcatg ataataattc ttagtattaa ttcggcaatc tgattctact cccccccagt
     6601 acctgatact acccccccag tagattaata gtgctatgat ttttcatatt cttgttaacc
     6661 aggtgttgcc atgtctcata caatccgtga taaacagaaa ctgaaagcgc gtgccagtaa
     6721 gattcagggc caggtcgtgg cgctcaagaa aatgctcgac gagccgcacg aatgcgctgc
     6781 agttttacaa cagattgctg ctatccgtgg agcggtaaac ggtctgatgc gggaagtgat
     6841 taaaggtcat ctgacggaac acatcgttca ccagggggat gagctaaaac gtgaagaaga
     6901 tctggatgtc gttctgaagg tgctggattc atatatcaaa taatttatta acgcgattgt
     6961 aaaactgccg tttttcctcg tttacaacgg gggcgctgga cattaccatc ctcctctgcg
     7021 atttatcatc gcaaccaaac gactcggggt gcccttctgc gtgaaggctg agaaataccc
     7081 gtatcacctg atctggataa tgccagcgta gggaagtcac ggaccaccag gtcattgctt
     7141 cttcacgtta tggcaggagc aaactatgca agtcgacctg ctgagttcag cgcaatctgc
     7201 gcacgcgtta cacctttttc accaacattc ccctcttgtg cactgcatga ccaatgatgt
     7261 ggtgcaaacc tttaccgcca ataccttgct ggcgctcggt gcatcgccag cgatggttat
     7321 cgaaaccgaa gaggccagtc agtttgcggc tatcgccagt gccttgttga ttaacgttgg
     7381 cacactgacg cagccacgcg cacaggcgat gcgtgctgcc gttgagcaag caaaaagctc
     7441 tcaaacacgc tggacgcttg atccagtagc ggttggtgcg ctcgattatc gccgccattt
     7501 ttgtcatgaa cttttatctt ttaaaccggc agcgatacgt ggtaatgctt cggaaatcat
     7561 ggcattagct ggcattgcta atggcggacg gggagtggat accactgacg ccgcagctaa
     7621 cgcgataccc gctgcacaaa cactggcacg ggaaactggc gcaatcgtcg tggtcactgg
     7681 cgaggtggat tatgttaccg atggacatcg tatcattggt attcacggtg gcgatccgtt
     7741 aatgaccaaa gtggtaggaa ctggctgtgc attatcggcg gttgtcgctg cctgctgtgc
     7801 gttaccaggc gatacgctgg aaaatgtcgc atctgcctgt cactggatga aacaagccgg
     7861 agaacgcgca gtcgccagaa gcgaggggcc aggcagtttt gttccacatt tccttgatgc
     7921 gctctggcaa ttgacgcagg aggtgcaggc atgaaacgaa ttaacgctct gacgattgcc
     7981 ggtactgatc cgagtggtgg tgcggggatt caggccgatc ttaaaacctt ctcggcactt
     8041 ggcgcttatg gttgctcagt tattactgca ctggtggcgc aaaatacccg tggcgtacag
     8101 tcggtgtatc gcattgagcc tgattttgtc gccgcccagc tcgattcggt gttcagcgat
     8161 gtgcgaatcg ataccactaa aatcggtatg ttggcggaaa ccgatattgt tgaagcggtg
     8221 gcagaacggt tgcaacgtta tcagatccaa aacgtggtac tcgacaccgt tatgctggca
     8281 aaaagcggcg acccgctgct ttcaccttcg gcggttgcta cgctgcgcag tcgattattg
     8341 ccacaggttt cattaataac gccaaacttg cccgaagctg ccgccttgct cgacgcgcca
     8401 cacgcgcgca ccgaacagga aatgctggaa caagggcgat cgctgttggc gatgggctgt
     8461 ggcgcagtgc taatgaaagg tggtcatctg gatgatgagc aaagcccgga ctggctgttt
     8521 acccgcgagg gtgaacaacg gtttaccgca ccgcgcatta tgaccaaaaa cacccacggc
     8581 actggttgta cactctctgc ggcgttggct gcactacgcc cgcgccatac aaactgggct
     8641 gacaccgtac aggaggcaaa aatctggctt tcatcggcgt tagcccaggc cgacacgctg
     8701 gaagttggtc acggtattgg tccggttcac cacttccacg cctggtggtg agcaccgtac
     8761 tatagctgct ttggttattg tcagcctttg gcactgacat tcagggatga aaccaatgca
     8821 gctaagaatt accagtcgaa aaaaactcac cgctctatta tgcgctcttg ggctaatttc
     8881 catcgttgca atttatccgc gtcagacggt gaattttttc tattcgacag cagttcagat
     8941 tacagactac atccacttct acggttatcg cccggttaaa tctttcgcta ttcgcattcc
     9001 tgccagttac accattcacg gattagatgt ttcgcgctgg caggagcgta tcgactggca
     9061 gcgggtggca aaaatgcgcg acaacgatat ccgcttacag tttgctttta ttaaggctac
     9121 ggaaggcgaa aagctggtgg acccctattt ttcgcgtaac tggcaattaa gccgcgaaaa
     9181 tggcctgctg cgcggggcgt atcattattt ctccccgtcg gtatccgctt cagttcaggc
     9241 gcgattattt ctgcaaacgg tggatttctc acaaggcgat ttccctgccg tgctggacgt
     9301 agaagaacgg ggaaaattat cggcaaaaga attacgcaag cgggtaagtc agtggctaaa
     9361 aatggtcgaa aaacgcacgg gaaaaaagcc gattatttac tcaggagccg ttttttatca
     9421 caccaatctg gcgggctatt tcaatgaata tccgtggtgg gtggctcact attatcaacg
     9481 tcgtccggac aatgacggca tggcctggcg cttctggcag cattccgacc gtggacaggt
     9541 agatggcatc aatggtccgg tggattttaa tgtgtttaat ggcacgggga tgagttgcag
     9601 gcattcgttg atgggattaa agaaacgcct taaataagaa gcagtgatat actcccacca
     9661 gactccctgg caggaaaaag acaaatggaa caagcgcata cccagctgat tgcccaactg
     9721 aacgagcgta ttttagcggc ggataacacg ccgctttata ttaagtttgc cgaaacggta
     9781 aaaaatgccg tgcgcagcgg ggtgctggag catggcaata ttttgcccgg tgagcgtgat
     9841 ttaagtcagt taaccggcgt ttcgcgcatt acggtgcgca aggcgatgca ggcgctggaa
     9901 gaagagggcg tggtgacgcg ttcgcgcggt tacggcacgc agatcaacaa catcttcgaa
     9961 tactcgttga aagaagcgag aggtttttct cagcaggtgg tattgcgcgg gaaaaagccc
    10021 gatacgctat gggttaataa gcgtgtcgtg aaatgtcccg aagaagttgc gcagcagctg
    10081 gcggtcgaag cgggaagtga tgtctttttg cttaagcgta ttcgctatgt cgatgaagaa
    10141 gcggtatcga ttgaggaatc gtgggttccg gcgcatttaa ttcatgatgt tgatgccatc
    10201 ggaatttctc tttatgacta tttccgcagc cagcacattt acccacaacg tacgcgttcc
    10261 cgcgttagcg cccggatgcc ggatgccgag tttcagtcac atattcagtt agatagcaaa
    10321 ataccggtgc tggtgatcaa gcaagtggcg cttgaccaac agcaacggcc tattgagtac
    10381 agcattagcc actgtcgcag cgatctatac gtttttgtgt gcgaggagta gttcctcgcg
    10441 cgttggcgca caatcaccgc cccgatgccc gacaacccac gacgccactg cattgcccag
    10501 taatacggca tccgccagtg gcagaccaga ggccagcccg gcaagtacgc cacctgcatg
    10561 actgtcgccc gccccaatgg tgtctacaac ttgcgttgga aatgccggaa tgcagccaga
    10621 agcgtcgttg ctgaaatacc atgcgccttc tttatcgaga cgaacgatca acggcgcggc
    10681 aaatttctcc tgccattgct tgccaagtgt tgttatctct gcggataaag caaaacgttc
    10741 ggcggcaatc tcagcctctt gacgattgag tgacactaaa ggtcgacagg ccatgatccg
    10801 cgccagtaat gcgtccggga tatcgccaat acgtgggcaa aaatcgataa acggcgtcac
    10861 gtcttgcagc ttttccagcc attccactaa caattcgccg cagggcgagg ccagttgata
    10921 accggaaaaa tagagcaggt tgccaggcgc aacggttaat cgtgccaacc attggcgatt
    10981 ccattgattt tccacaccgc tgaatgacat aaaagtgcgt tcgccatccg gctcaaccag
    11041 tgccagacac cagccgttat cgctttcggc gttatcgatc aggctgatta acccctcttt
    11101 cgccatccgg ttgcgaataa tctccgccca cacgccctga ccgagcggca aggcattacc
    11161 cgcttcgatg ccgaggcgct ttaacgccac ggcaatattc agtgcgcagc cgccaacgtt
    11221 aacgctctgc tgtttcagtt cgatatcgca cccacgccag gggagggcat aagcgtcggc
    11281 gatcacgtca atgaccgccg cgccgacgac catcaccggc tgacgcgtgg ttaattcagg
    11341 cagcagtgtg tgtaatcgag cgccgctcat accgcctccc gttgttgacg atatttcgcc
    11401 agcgctgtgg catagcggtt gaaatcaagc tgatttaccg catccagctc cgcctttaac
    11461 gcaggatcga cagcgttaat gccatgcaac gcgccgcaaa tcgccgtcgc catagcacct
    11521 atggtgtctg tgtcgccgcc aagattggcg cacaggacgg cgcagcggtt tgggtcggtt
    11581 tgtgccagtt caactagcgc aatggcgcac ggaacggact caatagtgct ggtgcctgcg
    11641 ccaacgacct ggtaaagctg ttcgctggcg gattcggtgc cgtcggcatt acgcacaatt
    11701 ttcagcgcaa tctccagacg tgccgccagt gaggcgctga acgtggtggt gcgtttttgc
    11761 tgtgcatgtc gcgcaattga aggcaatgaa tcaacaatcg ctgaccagcg ttctccgtca
    11821 atggcacgag aaatcgccca tgcgatgact accgcgcctg caaccgccag atcggattta
    11881 tgggtcgggc tggacgccag cgccacatca tcaataaagg aatcaacatc acgcgctgga
    11941 agcaaacaac ctaatggcga gacgcgcatc gctgcgccgt tggttacgcc gttgttttcc
    12001 agttcagcaa cgggtttacc gtcgcgaatg gcgttaagcg caatcttcga ggtcggacct
    12061 agtacgtttt tgttaaaggc gtcgaaacgc aacgcccagt cgagaatatt acgcccaatc
    12121 agattcggat cgatcttgcc ttcacattcc agtaacgcat ccgccagaca caggccatcg
    12181 aggtatcgtc ggtgaattcg gcgcggttaa aataacaggc cgcgttattc tcctttggtc
    12241 caggcagaaa acggtcaatc cagccaaagt gtgctttaac gcggctgcgt ggccaaagct
    12301 cggagggcat ccccatcgca tcccctaacg cctgcccata aagagcaccg agaatacgtt
    12361 ctgttttcat ttaacttccc cttgaatcgg aatttacggg agcatagtaa tgacagatat
    12421 tgcgcagttg cttggcaaag acgccgacaa ccttttacag caccgttgta tgactattcc
    12481 ttctgaccag ctttatctcc ccggacatga ctacgtagac cgcgtgatga ttgacaataa
    12541 tcgcccgcca gcggtgttac gtaatatgca gacgttgtac aacaccgggc gtctggctgg
    12601 cacaggatat ctttctattc tgccggttga ccagggcgtt gagcactctg ccggagcttc
    12661 atttgctgct aacccgctct actttgaccc gaaaaacatt gttgaactgg cgatcgaagc
    12721 gggctgtaac tgtgtggcat caacttacgg cgtgttggtg tcggtatcgc ggcgctatgc
    12781 gcatcgcatt ccatttctcg tcaaacttaa tcacaacgag acgctaagtt acccgaatac
    12841 ctacgatcaa acgctgtatg ccagcgtgga gcaggccttc aacatgggcg cggtggcggt
    12901 tggtgcgact atctattttg gctcggaaga gtcacgtcgc cagattgaag aaatttctgc
    12961 ggcctttgaa cgcgcgcacg agctgggcat ggtgacagtg ctgtgggcct atttgcgaaa
    13021 ctccgccttt aagaaagatg gcgttgatta ccatgtttcc gccgacctga ctggtcaggc
    13081 gaaccatctg gcggcaacca taggtgcaga tatcgtcaaa caaaaaatgg cggaaaataa
    13141 cggcggctat aaagcgatta attacggtta taccgacgat cgcgtgtaca gcaagttaac
    13201 cagcgaaaac ccgattgatc tggtgcgtta tcagttagct aactgctata tgggccgggc
    13261 tgggttgata aactccggcg gtgctgcagg cggtgaaact gacctcagcg atgcagtgcg
    13321 tactgcggtt atcaacaaac gcgcaggcgg aatggggctg attcttggac gtaaggcgtt
    13381 caagaaatcg atggctgacg gcgtgaaact gattaatgcc gtgcaggacg tttatctcga
    13441 tagcaaaatt actattgcct gatacgcctc atacgaacag caccaccccg taggacggat
    13501 aagacgttta cactgcatcc ggcaacctat gcctgatgcg acgcggaagc gtcttatcat
    13561 gcctacatag cactgccacg tatgtttaca ccgcatccgg cataaaaaca cgcgcacttt
    13621 gctacggctt ccctatcggg aggccgtttt tttgcctttc actcctcgaa caattttcat
    13681 attgttgttt ttgcgatcat tatctcgaca tttaaaagca aataatttca ttatattttg
    13741 aaatcgaaag caaacgacaa gatatgaaaa tgtacgtggt atcgacaaag cagatgctga
    13801 acaacgcaca gcgcggcggt tatgcggttc cggcattcaa tattcacaat ctcgaaacga
    13861 tgcaagtggt ggtggaaacc gctgccaacc tgcatgcgcc ggtcatcatc gccggaacgc
    13921 ctggcacatt tactcatgct ggtacagaaa atctgttggc gctggtcagc gcgatggcga
    13981 agcaatatca ccatccactg gcaattcatc tcgaccatca cacgaaattt gacgatatcg
    14041 ctcagaaggt tcgttctggc gtgcgctcag tcatgattga cgcctcgcat ttgccttttg
    14101 cgcaaaatat ttcacgggtc aaagaggtgg tggatttttg ccatcgcttt gatgtcagcg
    14161 tcgaggcgga gctggggcaa cttggcggcc aggaagatga tgtgcaagtc aatgaagtcg
    14221 atgcgttgta caccaacccc gcacaggcgc gtgaatttgc cgaggcaacc gggattgatt
    14281 ccctggcggt cgccatcggc acggctcacg gtatgtatgc cagcgcaccg gtgcttgatt
    14341 tttctcgact ggagaacatt cgccagtggg tgaacttacc gctggtgctg catggcgcgt
    14401 caggattatc gactaaggat attcagcaaa ccatcaaact ggggatatgc aaaatcaacg
    14461 ttgcaacgga gctgaaaaat gccttcttgc agtcgttaaa aaattacctg accgagcacc
    14521 ccgaagcgac cgatccccgg gattatttgc agtcggctaa atccgcaatg cgcgatgtgg
    14581 tgagcaaagt gattgccgat tgtggctgcg agggcagggc ataacgcacc tgccatttaa
    14641 caaggaaaaa acatgaaaac gttaattgcc cggcataaag ctggtgaaca tatcggcata
    14701 tgttcagtct gttctgccca tccgttggtt atcgaagcgg cgctggcatt tgatcgcaac
    14761 agcacgcgca aagtgctgat tgaagcaacg tcaaaccagg tcaatcaatt tggcggttat
    14821 accggaatga caccggcaga ctttcgcgaa tttgttttta cgattgccga taaagttggg
    14881 tttgcacgcg aacgcattat tctcggcggc gatcatctgg gaccaaactg ctggcagcaa
    14941 gaaaatgcgg atgcggcgat ggaaaaatcc gtcgagctgg taaaagcata tgttcgtgct
    15001 ggcttcagta aaattcatct tgatgcgtca atgtcctgcg cgggggatac cataccgtta
    15061 gcaccagaaa cggttgcgga acgagctgct gtgctttgct ttgctgcgga aagtgtggcg
    15121 acagattgcc agcgtgagca actgagctat gtcattggca ctgaagttcc ggttccgggc
    15181 ggtgaggcca gcgccattca gtcagtacac atcacccatg ttgaagatgc cgccaatact
    15241 ttacgtacgc atcaaaaggc ctttattgcc cgtgggctga cagaggcgtt aacacgtgtg
    15301 attgccatcg tggtgcagcc gggtgtggaa tttgatcaca gcaatattat ccattatcag
    15361 ccgcaggaag cgcaggcgct ggcgcaatgg atagaaaaca cccgaatggt ttatgaagca
    15421 cattctaccg attaccagac ccggacggct tattgggaat tagtccgcga tcactttgca
    15481 atattgaaag tcggtcccgc attaaccttt gctttacgcg aggcgatatt tgcactggca
    15541 caaattgagc aggaacttat cgcccccgaa aatcgcagcg gttgcctggc ggtaattgaa
    15601 gaagtgatgc tcgacgaacc gcaatactgg aaaaaatatt atcgcacggg ttttaacgat
    15661 tcattactgg atattcgtta cagcctgtcg gatcgtattc gttattactg gccgcatagt
    15721 cggattaaaa atagcgttga aacgatgatg gtgaatctgc aaggcgtgga catcccactg
    15781 ggcatgatta gtcagtatct tcccaaacaa tttgaacgca ttcagtccgg ggaattatca
    15841 gcaatgccgc atcagcttat tatgaataaa atttatgatg ttttgcgcgc ctatcgctac
    15901 ggctgtgcgg aataaggacg gtatatgact aacctgtttg ttcgtagcgg aatttctttt
    15961 gtcgatcgta gcgaagtttt aacctatatc ggtaatgaga tgctcgcgaa aggtgtggtt
    16021 catgatacct ggccacaggc attaattgcc agagaagcag aattccctac cgggataatg
    16081 cttgagcagc acgccattgc aataccgcat tgtgaggcga ttcatgctaa gtcgtcagcc
    16141 atttatctgt taaggccaac aaataaagtt cattttcagc aagcggatga tgataacgac
    16201 gtggcggtat cgttggttat tgcgttgatt gtggaaaatc cgcagcagca attgaaactt
    16261 ttacgctgtt tatttggcaa gttacaacag cccgatatcg tcgagacact aattactctt
    16321 cctgaaactc agttaaagga atacttcaca aagtatgttt tagattcaga cgaataaatc
    16381 cctctgtaac aataaaaagg actatttatg aaacgcaaga ttattgtcgc ttgcggaggc
    16441 gcggttgcga cctctacgat ggcggcggaa gaaattaaag agttgtgtca gagtcataat
    16501 attcctgttg aattaatcca gtgtcgggtt aatgaaatag aaacctatat ggatggtgtg
    16561 catttgatat gtaccactgc cagggtggat cgtagttttg gcgatattcc gttagttcac
    16621 ggcatgcctt ttgtttctgg tgtcggtatc gaagcattac aaaataaaat tctgactatc
    16681 ttacagggat gacctatgtt ttcagaagtc atgcgttata ttctcgacct cggccctaca
    16741 gtgatgctgc cggttgtcat cattattttt tctaaaatat taggcatgaa ggcaggcgat
    16801 tgctttaaag cgggtctgca tatcgggatt ggctttgttg gcattggcct tgtgattggc
    16861 ttaatgctgg attccattgg tccggcggcg aaagcgatgg cggaaaattt cgacctgaat
    16921 ctgcatgtgg tcgatgtcgg ctggccgggc tcttcaccaa tgacctgggc gtcgcaaatt
    16981 gcgctggtgg cgattccgat tgcgatattg gttaacgtgg cgatgttact gacccgtatg
    17041 acgcgggtgg taaatgttga tatctggaat atctggcata tgaccttcac cggcgcgttg
    17101 ctgcacctgg caaccggttc atggatgata gggatggcgg gtgtggtaat tcacgcggcg
    17161 tttgtttata agctcggcga ctggtttgcc cgcgataccc gaaatttctt tgagctggaa
    17221 ggtattgcta ttccgcacgg tacgtcggcg tatatggggc cgattgcggt gctggtcgat
    17281 gctatcatcg agaaaatccc aggcgttaac cgaattaaat ttagcgccga cgatattcag
    17341 cgcaaatttg gcccttttgg cgaacctgtc accgtgggtt ttgtgatggg gctgattatc
    17401 ggcatcctcg cgggttacga tgtcaaaggt gtattgcagc tggcggtaaa aacggcggca
    17461 gttatgctgc tgatgccacg gatgattaaa cccatcatgg atggtttaac gcccatcgct
    17521 aagcaggccc gtagtcgttt acaggcgaag ttcggcggtc aggagttcct gattggcctg
    17581 gatcccgcat tactgctggg gcatacggcg gtggtatcgg caagcctgat ttttattccg
    17641 ctcaccattt taattgctgt ttgtgttccg ggtaatcagg tactgccgtt tggcgatctt
    17701 gccactatcg gcttcttcgt ggcgatggcg gtcgcggtgc atcgtggaaa tctgttccgc
    17761 accttaatct cgggtgtcat cattatgagc atcaccctgt ggatcgcgac gcaaactatt
    17821 ggtttgcaca cccaaccggc ggctaatgct ggggcgttaa aagccggggg tatggtggct
    17881 tcaatggatc agggcggttc tcccattacc tggttactga ttcaggtttt ctccccgcaa
    17941 aatattcccg gtttcattat tatcggcgca atttatctga ccggtatttt catgacctgg
    18001 cgtagagcgc gtggttttac taaacaagag aaagccgttc tcgcagaata atttttacct
    18061 gagggggagg ttatccccct taacaa
//
LOCUS       DADLTY010000081        18045 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.081, whole genome shotgun sequence.
ACCESSION   DADLTY010000081 DADLTY010000000
VERSION     DADLTY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.081"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            543..1124
                     /locus_tag="JN334_002319"
     CDS             543..1124
                     /locus_tag="JN334_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY7438442.1"
                     /translation="MKQQEEHNNKIVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTH
                     VIKSVKDQQNTFIFTEFNPAKTKYFILNNGSVALAGRVLSIDATENGSVIHISLVNLL
                     STPISNIGFNATWGGEKPVDAKEFARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVS
                     PNNLGYLKLAINMENIQFDNLPSAENRQKRSKK"
     gene            1168..5830
                     /locus_tag="JN334_002320"
                     /pseudo
     CDS             1168..5830
                     /locus_tag="JN334_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            6198..7853
                     /gene="lldP"
                     /locus_tag="JN334_002321"
     CDS             6198..7853
                     /gene="lldP"
                     /locus_tag="JN334_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HAY7438443.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7853..8629
                     /gene="lldR"
                     /locus_tag="JN334_002322"
     CDS             7853..8629
                     /gene="lldR"
                     /locus_tag="JN334_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HAY7438444.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8626..9816
                     /gene="lldD"
                     /locus_tag="JN334_002323"
     CDS             8626..9816
                     /gene="lldD"
                     /locus_tag="JN334_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HAY7438445.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10012..10485
                     /gene="trmL"
                     /locus_tag="JN334_002324"
     CDS             10012..10485
                     /gene="trmL"
                     /locus_tag="JN334_002324"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAY7438446.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10538..11359)
                     /gene="cysE"
                     /locus_tag="JN334_002325"
     CDS             complement(10538..11359)
                     /gene="cysE"
                     /locus_tag="JN334_002325"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAY7438447.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11439..12458)
                     /gene="gpsA"
                     /locus_tag="JN334_002326"
     CDS             complement(11439..12458)
                     /gene="gpsA"
                     /locus_tag="JN334_002326"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAY7438448.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12458..12925)
                     /gene="secB"
                     /locus_tag="JN334_002327"
     CDS             complement(12458..12925)
                     /gene="secB"
                     /locus_tag="JN334_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAY7438449.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12988..13239)
                     /gene="grxC"
                     /locus_tag="JN334_002328"
     CDS             complement(12988..13239)
                     /gene="grxC"
                     /locus_tag="JN334_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAY7438450.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13381..13812)
                     /locus_tag="JN334_002329"
     CDS             complement(13381..13812)
                     /locus_tag="JN334_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAY7438451.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14057..15601
                     /gene="gpmM"
                     /locus_tag="JN334_002330"
                     /gene_synonym="pgmI"
     CDS             14057..15601
                     /gene="gpmM"
                     /locus_tag="JN334_002330"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAY7438452.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15611..16894
                     /gene="envC"
                     /locus_tag="JN334_002331"
     CDS             15611..16894
                     /gene="envC"
                     /locus_tag="JN334_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HAY7438453.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16898..17857
                     /locus_tag="JN334_002332"
     CDS             16898..17857
                     /locus_tag="JN334_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HAY7438454.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17844..18045)
                     /locus_tag="JN334_002333"
                     /pseudo
     CDS             complement(17844..18045)
                     /locus_tag="JN334_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4545 a         4662 c         4674 g         4164 t
ORIGIN      
        1 actcccttta cttcgattta ccgctggttg atattctgaa aatgcatttt taccgaaata
       61 gccatctata aacaataaaa atatatagaa gatatcattt gttaattaac aagcatttac
      121 gtctcttttt ttcggattta attgtgaaat tttaatcatc atattcacaa aatcaattat
      181 ctgtgaaatt tttcccagac atggtttctt tcatatttga catttattaa taatcatcat
      241 tagaatccac gaaaaataat atattcatac aaacacagac atcttttgaa acgaatgata
      301 catttttatg atgaatgctt aaaacaatat ttaatcgaca cacatcactg tcattaatgt
      361 aaagcaacat agctcaccaa taatacaatg gacaactcta ccggcattga gcgctggtcg
      421 gaggaaataa ggatttttac atgaacttga agaagatttt tttcagtgct gtcactgttt
      481 cagttctctg cgcattaacg ggatgtgact acattgaaga aggcaagcca gaaagtagtt
      541 tactgaaaca acaagaagaa cataataata aaattgtgct acttgaaaaa caacaagcac
      601 agctaaagag tcaactcgaa actattcaaa aacaacaaac tggcataata aacagtacaa
      661 agactttaac tcacgtaatt aaatccgtta aagaccaaca aaatactttt atttttaccg
      721 agtttaaccc agcaaaaacg aagtatttca tcctgaacaa cggttcggtg gctttagcgg
      781 gtcgagtgtt atccattgac gccacagaga atggtagtgt tattcatatc tcactggtca
      841 acttattaag tacacctatc tcaaacattg gttttaatgc gacatggggt ggtgaaaaac
      901 ctgtcgatgc caaagagttt gcccggtggc aacaattact tttcaacaca tcaatgaaat
      961 ccacattgaa attattacca ggtcaatggc aagacattaa tttgaccctg aagggtgtat
     1021 cgcccaataa cctgggatat ctgaagttag ccatcaacat ggaaaatatt cagttcgaca
     1081 atcttccctc tgctgaaaat cggcagaaaa gaagcaaaaa ataaatgcag ttagtacgct
     1141 tataacaagg aatagcgagc atcattaatg aacaaaatat ttaaagttat ctggaaccct
     1201 gcgacaggga attatactgt taccagcgaa acggcaaaaa gccgtggcaa gaaatctggg
     1261 cgcagtaagc tgttaatttc tgcgctggtt gcgggtggaa tgttgtcgtc gtttggggca
     1321 ttggcgaatg ccgggaatga caacggtcag ggtgttgatt acggtagtgg atcagctggc
     1381 gacggctggg ttgctatagg caaaggggcg aaagcaaata cttttatgaa caccagtggt
     1441 tccagtactg ctgtgggtta tgacgctata gctgaaggcc aatatagctc tgccatcggg
     1501 tcaaaaaccc atgcgattgg tggtgcatca atggcctttg gggttagtgc aatatcagaa
     1561 ggcgatagaa gtatagcact gggtgcctct tcgtattcat tgggccaata ctcaatggcc
     1621 ctcggccgtt attcaaaagc attgggtaaa ttgtctattg ctatggggga ctcttccaaa
     1681 gcggaaggag caaacgccat tgccctgggg cgtagcagtg tagctagcgg tactgacagc
     1741 ctcgcatttg gcagacaatc acttgccagc gcagcgaacg ctattgcgat aggtgctgag
     1801 accgaagccg ctgaaaatgc aactgctatt ggcaataatg cgaaggcaaa agggactaat
     1861 agcatggcaa tggggttcgg aagccttgcc gataaagtca atactatcgc attaggaaat
     1921 ggcagccagg ctctggcaga taatgcaatc gccataggcc agggcaacaa agctgatggc
     1981 gtggatgcca tcgctctggg taatggtagc cagtcgagag gcttaaacac cattgcctta
     2041 ggcacagcca gtaatgcaac tggtgataag agtcttgcgc ttggtagtaa tagcagtgcc
     2101 aacggtatta actctgtcgc gctgggcgca gattccattg cggatttaga caataccgtc
     2161 tctgtcggca atagttcatt aaaacgcaag atcgttaatg tgaaaaatgg cgcgatcaag
     2221 tctgacagtt acgatgccat taatggttca cagctttatg ccattagcga ctcggtagca
     2281 aaaaaggctt ggaggagggg ctgcagtaga tgttgatgac ggtactgtta cagcaccaac
     2341 ctacaattta aaaaatggta gcaaaaataa cgtaggggct gcgctcgctg tacttgatga
     2401 aaacaccctg caatgggacc aaaccaaagg caaatacagc gctgctcatg gtactagtag
     2461 cccaactgcc agcgtaatca ccgatgttgc ggatggcacg atttcagcct ccagtaagga
     2521 tgcggttaac ggttcccaac tgaaagctac caatgacgat gtcgaagcca acaccaccaa
     2581 tatcaccaac ctgacggatt ccgttggtga ccttcaggct gatgccctgc tctggaacga
     2641 aactaaaaag gcattcagtg cagctcacgg ccaggatacc accagcaaaa tcaccaacgt
     2701 taaagatgcc gacctgacgg ctgacagcac tgatgctgtt aacggctctc agctgaaaac
     2761 caccaacgat gctgtggcga cgaataccac caatatcgcc aataacactt ccaatattgc
     2821 cactaacacc accaacatct ctaacctgac tgagacggtg actaatcttg gtgaggatgc
     2881 gctgaaatgg gataaggaca atggtgtatt cacggcagct catggcaccg agaccaccag
     2941 caaaataacc aacgttaaag atggcgacct gacgactggc agcaccgatg ccgttaacgg
     3001 ctctcagctg aaaaccacca acgatgccgt ggcgacgaat accaccaata tcgccactaa
     3061 caccaccaac atctctaatc tgactgagac ggtgactaat cttggtgagg atgcgctgaa
     3121 atgggataag gacaatggtg tcttcactgc agctcatggc aacaataccg ccagcaaaat
     3181 caccaatatc ctggacggca cagtcactgc aaccagttcc gatgccatta acggtagcca
     3241 gctttatgac ttaagcagca atatcgccac ctacttcggc ggcaatgctt ctgtgaatac
     3301 tgacggtgtg tttaccggtc caacctacaa aatcggtgaa acaaattatt ataacgtcgg
     3361 cgatgcactg gctgcgatta actcctcatt tagcacgtct ctcggcgatg ctctgctttg
     3421 ggatgccacc gcaggtaaat tcagtgccaa acacggtact aatggtgacg caagcgtgat
     3481 cactgatgtc gcagatggtg aaatttcaga ctccagttct gacgcagtaa acggctcaca
     3541 actccacggc gtgagcagtt atgttgttga tgcgctgggg ggtggtgccg aagtcaatgc
     3601 agacggcacc atcactgcgc cgacgtacac cattgctaat gctgattacg ataatgtcgg
     3661 tgatgccctg aatgctatcg ataccactcc tgacgacgct ctgctctggg atgcggacgc
     3721 cggtgaaaat ggtgcattta gcgccgctca cggaaaagat aaaactgcca gtgtaatcac
     3781 taacgtcgct aacggtgcaa tctctgctgc cagcagcgac gcgattaacg gctcacaact
     3841 ctataccacc aataagtaca tcgctgatgc gctgggtggt gacgcagaag tcaacgctga
     3901 cggcaccatc accgcaccga cttacaccat tgcgaacgcc gagtacaaca acgtcggtga
     3961 cgccctggat gcgcttgatg ataacgccct gctgtgggat aagactgcca atggcggtgc
     4021 tggagcctac aatgccagcc atgacggtaa agccagcatc atcactaatg tcgctaatgg
     4081 cagtattagt gaggacagta ccgatgcagt gaacggttct cagttgaatg cgacgaatat
     4141 gatgattgag cagaacaccc aaattatcaa tcagctcgct ggtaacaccg acgcaaccta
     4201 tatcgaagaa aatggcgctg gtatcaacta cgttcgtact aacgacaacg atttagcctt
     4261 taacgatgca agcgcctctg gtgtcggcgc tacagctgta ggttataacg ctgtcgcgtc
     4321 tggtgccagc agcgtagcca ttggtcagaa cagcagcagc accgttgata ccggtattgc
     4381 gctgggtagc agctccgttt ccagccgtgt gattgccaaa ggttctcgtg acactagcgt
     4441 aacggaaaat ggcgtggtga ttggttatga caccactgac ggcgaactgc taggtgcatt
     4501 gtcaattggt gatgacggta aataccgcca aatcatcaac gtagctgatg gttcagaagc
     4561 ccatgacgcc gttacggttc gccagttgca gaacgctatt ggagcggtcg ccactacgcc
     4621 aaccaagtac tttcacgcca actcaacggc agaagactca ctggccgttg gtgaagactc
     4681 gctggcaatg ggtgcgaaaa ctgtcgttaa tggtaatgca ggtattggta tcggtttgaa
     4741 cactctggtt ctggctgatg cgatcaacgg cattgctatc ggcagtaacg cacgcgcaaa
     4801 ccatgcaaac agtatcgcaa tgggtaacgg ttctcagacc acccgtggtg cacagactgg
     4861 ctacaccgcc tacaacatgg acgcaccgca gaactctgta ggtgagtttt ctgtcggtag
     4921 tgaagacggt caacgtcaga tcacaaacgt cgcagctggt tcagcggata ccgatgcggt
     4981 taacgtgggt cagttgaaag tcactgatga gcgcgtagcg caaaataccc agagcattac
     5041 taacctgaac aatcaggtca ctaatctgga tactcgcgtt actaatatcg aaaacggtat
     5101 tggcgacatt gtcaccaccg gtagcaccaa gtacttcaag accaacaccg atggcgtaga
     5161 tgccaacgcc cagggtaaag atagcgttgc tattggttct ggttccattg ctgccgctga
     5221 caacagcgtc gcactgggta ccggttccgt tgcagaggaa gaaaatacaa tctctgtagg
     5281 ttcttctact aaccaacgcc gtatcaccaa cgtagctgcc agtgttaatg ccaccgatgc
     5341 ggttaacgtg tcgcagctga aatcttctga agcaggcgga gtgcgctacg acaccaaagc
     5401 tgatggttct atcgactata gcaatatcac cctcggtggt ggcaacggca gtacgactcg
     5461 tatcagcaac gtctccgctg gcgtcaacaa caacgacgcg gtgaactacg cgcagttgaa
     5521 gcaaagcgcg caggaaacga agcaatacac cgatcagcgg atggttgaga tggataacaa
     5581 actgtctaaa actgaaagca agttgagcgg tggtatcgct tctgcaatgg caatgaccgg
     5641 tctgccgcag gcttatacac cgggtgccag catggcttct attggtggcg gtacttacaa
     5701 cggtgaatcg gcagttgctt taggtgtatc gatggtgagc gccaatggtc gttgggtcta
     5761 caaattacaa ggtagtacca atagccaggg tgaatactcc gccgcactcg gtgccggtat
     5821 tcagtggtaa tgatccatta agttagtgtg actaagggca aacgccaaat tgcctgatgc
     5881 gttacgcgca tcaggcaaga taaggcgctc cgggtcaaca atctaagggc aattctctga
     5941 tgaggattgc ccttttcttt accagacatc tccccccaca agaattggcc ctaccaattc
     6001 ttcgcttatc tgacctctgg ttcacaattc cccaattaaa actcacatca atattgccaa
     6061 tacataacat ttagttaacc attcattgtc attatcccta cacaacacaa ttggcagtgc
     6121 cacttttaca caacgtgtga caaggagatg agcaacagac tcattacacg atgtgcgtgg
     6181 actctaggag acctgcaatg aatctctggc aacaaaacta cgatcccgcc gggaatatct
     6241 ggctttccag tctgatagca tcgcttccca tcctgttttt cttctttgcg ctgattaagc
     6301 tcaaactgaa aggatacgtc gccgcctcat ggacggtagc aatcgccctt gccgtggctt
     6361 tgctgttcta taaaatgccg gtcgctaacg cgctggcctc ggtggtttat ggcttcttct
     6421 atggtttgtg gcccatcgcg tggatcatta ttgcagcagt gttcgtctat aagatctcgg
     6481 tgaaaaccgg gcagtttgac atcattcgct cgtctattct ttcgataacc cctgaccagc
     6541 gtctgcaaat gctgatcgtc ggtttctgtt tcggcgcttt ccttgaagga gccgcaggct
     6601 ttggcgcacc ggtagcaatt accgccgcat tgctggtcgg cctgggtttt aaaccgctgt
     6661 acgccgccgg actgtgcctg attgttaaca ccgcgccagt ggcatttggc gcgatgggca
     6721 ttccaatcct ggttgccgga caagtaacag gcatcgacag ctttgagatt ggccagatgg
     6781 tggggcgtca gctaccgttt atgaccatta tcgtgctgtt ctggatcatg gcgattatgg
     6841 acggctggcg cggtatcaaa gagacgtggc ctgcggtcgt ggttgcaggc ggctcgtttg
     6901 ccatcgctca gtaccttagc tctaacttca ttgggccgga gctgccggac attatctctt
     6961 cgctggtatc actgctctgc ctgacgctgt tcctcaaacg ctggcaacca gttcgcgtat
     7021 tccgcttcgg tgacttgggg gcgtcacagg ttgatatgac gctggcccac accggttaca
     7081 ccgcgggtca ggtgttacgt gcctggacac cgttcctgtt cctgaccgct accgtaacac
     7141 tgtggagcat cccgccgttt aaagccctgt tcgcatcggg tggtgcgctg tatgagtggg
     7201 tgatcaacat tccggtgccg tacctcaata aactggttgc ccgtatgcca ccagtggtca
     7261 gcgaggctac agcctatgcc gccgtgttta agtttgactg gttctctgcc accggcaccg
     7321 ccattctgtt tgctgcactg ctctcgattg tctggctgaa gatgaaaccg tctgacgcta
     7381 tcagcacctt cggcagcacg ctgaaagaac tggctctacc catctactcc atcggcatgg
     7441 tgctggcgtt cgcctttatc tcgaactatt ccggactgtc atcaacactg gcactggcac
     7501 tggcgcacac cggtcatgca ttcaccttct tctcgccgtt cctcggctgg ctgggggtat
     7561 tcctgaccgg gtcggatacc tcatctaacg ccctgttcgc cgcgctgcaa gccaccgcag
     7621 cacaacaaat tggcgtctct gatctgttgc tggttgccgc caataccacc ggtggcgtca
     7681 ccggtaagat gatctccccg caatctatcg ctatcgcctg tgcggcggta ggcctggtgg
     7741 gcaaagagtc tgatttgttc cgctttactg tcaaacacag cctgatcttc acctgtatgg
     7801 tgggcgtgat caccacgctt caggcttatg tcttaacgtg gatgattcct taatgattgt
     7861 tttacccaga cgcctgtcag acgaggttgc cgatcgtgtg cgggcgctga ttgatgaaaa
     7921 aaacctggaa gcgggcatga agttgcccgc tgagcgccaa ctggcgatgc aactcggcgt
     7981 atcacgtaat tcactacgcg aggcgctggc aaaactggtg agcgaaggcg tgctgctcag
     8041 tcgacgcggc ggcgggacgt ttattcgctg gcgtcatgac acatggtcgg agcaaaacat
     8101 cgtccagccg ctaaaaacgc tgatggccga tgatccggat tacagtttcg atattctgga
     8161 agcccgctac gccattgaag ccagcaccgc atggcatgcg gcaatgcgcg ccacacctgg
     8221 cgacaaagaa aagattcagc tttgctttga agcaacgcta agtgaagacc cggatatcgc
     8281 ctcacaagcg gacgttcgtt ttcatctggc gattgccgaa gcctcacata acatcgtgct
     8341 gctgcaaacc atgcgcggtt tcttcgatgt cctgcaatcc tcagtgaagc atagccgtca
     8401 gcggatgtat ctggtgccac cggttttttc acaactgacc gaacaacatc aggctgtcat
     8461 tgacgccatt tttgctggtg atgccgacgg ggcgcgtaaa gcaatgatgg cgcaccttag
     8521 ttttgttcac accaccatga aacgattcga tgaagatcag gctcgccacg cacggattac
     8581 ccgcctgccc ggtgagcata atgagcattc gagggagaaa aacgcatgat tatttccgca
     8641 gccagcgatt atcgcgccgc agcgcaacgc attctgccgc cgttcctgtt ccactatatg
     8701 gatggtggtg catattctga atacacgctg cgccgcaacg tggaagattt gtcagaagtg
     8761 gcactgcgcc agcgtattct gaaaaacatg tccgacttaa gcctggaaac gacgctgttt
     8821 aatgagaaat tgtcgatgcc ggtagcactg gcaccggtgg gtttgtgtgg catgtatgcg
     8881 cgtcgtggcg aagttcaggc agccaaagcg gcggacgcgc atggcattcc gtttactctc
     8941 tcgacggttt ccgtttgccc gattgaagaa gtcgcgccag ccatcaagcg cccaatgtgg
     9001 ttccagcttt atgtactgcg cgatcgcggc tttatgcgta acgcgctgga gcgagcaaaa
     9061 gcagcgggtt gttcgacgct ggttttcacc gtggatatgc cgacaccggg cgcacgctac
     9121 cgtgatgcgc attcaggtat gagcggccca aatgcggcaa tgcgccgcta cttgcaggcg
     9181 gtgacgcatc cgcaatgggc gtgggatgtg ggcctgaacg gtcgtccaca tgatttaggt
     9241 aatatctcag cttatctcgg caaaccgacc ggactggaag attacatcgg ctggctgggg
     9301 aataacttcg atccgtccat ctcatggaaa gaccttgagt ggatccgcga tttctgggat
     9361 ggcccgatgg tgatcaaagg gatcctcgat ccggaagatg cgcgcgatgc agtacgtttt
     9421 ggtgctgatg gaattgtggt ttctaaccac ggtggccgcc agctggacgg tgtactctct
     9481 tccgctcgtg cgttgcccgc tattgcagat gcggtgaaag gtgatatcgc cattctggcg
     9541 gatagcggaa ttcgtaacgg gcttgatgtc gtgcgtatga ttgcgctcgg tgccgacacc
     9601 gtactgctgg gtcgtgcttt cctgtatgcg ctggcaacag cgggccaggc gggtgtagct
     9661 aacctgctaa atctgatcga aaaagagatg aaagtggcga tgacgctgac tggcgcgaaa
     9721 tcgatcagtg aaattacgca agattcgctg gtgcaggggc tgggtaaaga gttgcctacg
     9781 gcactggctc cgatggcgaa agggaatgcg gcatagtcgt ttgcgccccc tcaccctaac
     9841 cctctccctc agggagaggg gaccgttcgg cgctgtatgt actccctcac tctgaaacga
     9901 caccgcactc tttttttctc cctcgcccct ccggggaggg ccggggtgag gggaaaaggc
     9961 cgcacggttc accaattttc tgctatcctg cccccgcact aagggggcag tatgctaaac
    10021 atcgtacttt acgaaccaga aattccgcca aatactggca acatcatccg tctttgcgct
    10081 aataccggct ttcgtctaca tatcatcgaa ccgatgggat ttgcctggga cgacaagcgc
    10141 ctgcgccgcg cggggctgga ctatcacgag tttaccgccg ttacgcgtca tcatgactat
    10201 cgcgcgttcc tcgaagcaga aaatccccag cgcctgtttg ccctcaccac gaaaggtacg
    10261 cctgctcaca gcgccgtaag ctatcaggat ggcgattatc tgatgttcgg cccggaaaca
    10321 cgcggcctgc cagcgagcat tcttgatgcc ctgcccgctg aacaaaaaat tcgcattccg
    10381 atggtgccgg acagccgcag catgaatctg tccaatgcgg tgtcggtagt ggtgtatgaa
    10441 gcctggcggc agctgggata tccgggagcg ttgttgagag attagttact gtatgccgga
    10501 tagatgatta catcgcatcc ggcacgatca caggacatta gatcccatcc ccatactcaa
    10561 atgtatggtt aataccgttg aaatgctggt ccatatccat tgatggctta tcgctgtctg
    10621 gtttaccgac aatacgagcc ggaacgccag cggcggttgt atgcggcggc accggttgca
    10681 gcaccacgga acctgcgcca atcttcgcgc cgcgaccaac ttcaatattg ccgaggattt
    10741 tcgcgcccgc gccaatcatc acaccttcac gaattttcgg gtgacggtca ccaccagatt
    10801 tacccgtacc gccaagcgtc acagattgca gaatcgatac gtcgttttca attaccgccg
    10861 tttcacccac aacgatgccc gtggcgtggt cgagcataat gccacggcca atttttgctg
    10921 ccgggtgaat atcgacctgg aacgtcacag aaacctggtt ttgcagaaag attgccaatg
    10981 cgcgacgccc ctgattccac aaccagtgac cgatgcgata ggcctgcaag gcatgaaaac
    11041 ccttcaggta taacaacggg gttgagtatt tatcgactgc cgggtcgcgg gtacgcaccg
    11101 cctgaatatc acaggccgca gaggcgatca tttccgggtc agcggcgtag gcttcttcaa
    11161 ccacttcacg gatagcaata gcaggcataa ttggcgatga cagcttgttc gccagcatgt
    11221 agctcagtgc actgccaagg ttttcgtgct tgagtagcgt cgcgtggtaa aaactggcca
    11281 gcattggctc acagtccgcc agcgttctgg cttcggcttt aatattgttc cagacaattt
    11341 ccagttcttc acacgacatt gcttactcca cacgatgaga taatgaccgg cccgttctgc
    11401 gcgggccggg tcatagcggt aacaaaggtt ccctggggtt agtgactgct gcgctcgtcc
    11461 ttgcgtgcac gacctaataa tgtcaatgct gcctcgcgcg cgttttttcc gcaatataat
    11521 acttgataaa tttcctcggt tattggcatt tcaacgccga agcgatgcgc cagttcgcgg
    11581 acttctttcg tattgcggta gccttccacc acctgaccaa tcttctcctg cgcgctttgt
    11641 acatccatgc cctgaccgag catcatacca aaacggcggt tacgcgactg gttgtcggta
    11701 caggtaagca ccagatcgcc cagccccgcc atgcccataa aggtggcagg atcggcaccc
    11761 agcgccgcac caagacgcga catttcagcc agcccacggg tgatcagcgc cgtacgcgca
    11821 ttcgcaccaa aaccgatacc gtcagacatc cccgcgccaa tggcaatgac gtttttcacc
    11881 gcgccgccaa gctgcacgcc aatgaaatcc gggttgctgt aaacgcggaa acttttgccg
    11941 cagtgcagca gctgctggag atcatcggca aaggtctgat cggtcgaggc cagcgaaata
    12001 gctgtcggta aacctgccgc cagttctttc gcaaacgttg ggccagagat aaccgccagc
    12061 ggaatttgat cgcctaaggc ctcacgcgcc acgtcctgta acagacgtcc ggtttccgct
    12121 tccagccctt tggtcgccca caccagacgc gcatcaggac gcatcagcgg tttaatctgg
    12181 cgcagcactt caccaaagac atggctgggt acgacgacga gaatattacg actggctgcc
    12241 agcgcagtgg cgagatcgct ttcaagatgg agcgtatcgg gaaaaggcac atcggggaga
    12301 aacgcggcgt tacagcggtc gcgttcaagc gttgcgatat gttcagggtc atggccccag
    12361 aggacaacct cgtggccatt tcttgccagg gtaatggcaa gagcggtgcc gtacgagccg
    12421 gcaccgatca cagtcattga agcattacgt tggttcatca ggcatcctga tgttcttcag
    12481 taccttcgcc agcctgctgc tgcaaatagt tcatgaacag cgcatcgaag ttaaccggcg
    12541 caaggttcag ttgcgggaat gtaccgcggg ataccatgct ggtgatgcac tcacgagcat
    12601 acgggaacag aatgttcggg cagtatgcgc ccaggcaatg tgccatctgg gtgccttcga
    12661 tacccgcgat ggagaaaata ccgccctgct gaacttcaca caggaacgcg gtttcttcgc
    12721 ccaaggaggc cgttacggta acacgcagta ccacttcgta tacgtcatct gccagttggg
    12781 aagatgccgt atccagatca agtttaactt ctggttgcca atctttctgg aaaacgtgcg
    12841 gcgcgttcgg cgcttcgaaa gagatatcct tggtataaat acgttggatc tggaaagtca
    12901 tttcagtgtt gttttgttct gacatgtgta gaaaaccctt aagtgttgtc cttaaatact
    12961 gcgcaatgta ataccaacgt tcacacgtta tttcagcagg ggatccagtc caccacgtgc
    13021 atccaatgca tacaagtcat cacagccgcc aatgtgctgt gcgtcaataa aaatctgggg
    13081 aaccgtggtg caaccgctgc gtttgatcat ctcttcacgc ttggcggcat tgccatcgat
    13141 cggcaactcc tggaaactca cgcccttgct gctcagcagt gcttttgcac gatggcaata
    13201 cgggcaggtt tctttggtat agatttcaac attggccata atttagctcc tgttttttta
    13261 ccctgaaatt tcatgttgca gggcggctgc aaaatggagt gtccccagaa gcttataaca
    13321 ataagtcgct gggggcctaa ttgcagctaa cggcctgcat catgaaagac gacaggtaaa
    13381 ttatttgccg cgcaccagag gcaagttttc gccagcccag ccagcaacgc cctctttcag
    13441 cacgaatact tgcgcaaaac cagctttcgt cagcgcgttt gcaggctcct ggcactgcat
    13501 gccagaaccg tctaccacga taaccgattt gtctttgtgc ttctcaagct caccaacatt
    13561 gttggctttg atttcgctcg gcaacagatt gatagaacct gcgatatggc ctttacggaa
    13621 gtcatcacgc tgacgtaaat ccacaaccac agcgtcttct ttgttgatca gacgcgtagc
    13681 ttcaccacga gtaatcacct tcactttcga ggtcaggctc ttaaacgtag tcacaagaac
    13741 cgccaccagt aacgcgatcc aggcgatact cagtatggga tgacggccaa caaattgcat
    13801 aatttcttgc atggggggta acaactcccg acgtagtgat taaaaaaacc aggaaaggag
    13861 tatacctgcg cggtgcggca aatacagcca gcgcgttaac tggaatgcaa ttttgcgggg
    13921 cacgacgaaa aaaaagcgca aatctggcat cggcttgccg cgagcggtcg tatattttga
    13981 tcttcagagg ctattttatc gattcagctg tagtaaaatt acgcaaattt tgactcttga
    14041 gtatgaggtt gtcgcaatgt cggtttctaa aaaacctatg gtactggtga ttctggatgg
    14101 ctatggctat cgtgaagaac agcaggataa cgccattttt agtgctaaaa ccccggtaat
    14161 ggatgcactg tgggccaatc gtccgcatac cctgatcgac gcttccggtc tggaagtcgg
    14221 tctgcctgac cgtcagatgg gtaactccga agtaggccac gttaacctgg gtgccggccg
    14281 catcgtgtat caggacctga ctcgtctgga cgttgaaatc aaagatcgcg ctttctttgc
    14341 taatccagtg ctggctggcg cggtacataa agcgaaaaac gcaggtaaag cagtacacat
    14401 tatgggtctg ctctccgcag gcggcgtaca cagccacgaa gatcacatca tggcgatggt
    14461 agaactggca gctgaacgcg gcgcagaaaa aatctacctg cacgcattcc ttgacggtcg
    14521 cgacaccccg ccgcgcagtg ctgaatcctc gctgaaaaaa tttgaagaga aatttgctgc
    14581 gctgggtaaa ggccgcgtag cgtccatcat tggtcgttac tacgcgatgg accgcgataa
    14641 ccgttgggat cgcgttgaaa aagcctatga cctgctgact ctggcgcagg gtgagttcca
    14701 ggccgatact gccgttgctg gtttgcaggc agcttatgct cgcgacgaaa acgacgagtt
    14761 cgtgaaagcg accgttattc gtgcagaagg tcagccagat gcggcaatgg aagacggcga
    14821 tgcgctgatt ttcatgaact tccgtgctga ccgcgcgcgt gaaatcactc gtgcattcgt
    14881 gaacgctgat ttcgatggct tcgcgcgtaa gaaagtggtt aacgtcgatt tcgtgatgct
    14941 gaccgaatac gccgctgaca tcaaaactgc ggttgcttac ccacccgctt ccctggttaa
    15001 caccttcggc gagtggatgg cgaaaaacga caaaactcag ttgcgtattt ccgaaaccga
    15061 aaaatatgcc cacgttactt tcttcttcaa cggtggcgta gaagagtcgt tcaaaggcga
    15121 agatcgcatt ctgatcaact caccgaaagt ggctacctac gatctgcaac cggaaatgag
    15181 ctccgcagag ctgaccgaaa aactggttgc ggccatcaag agcggcaaat acgacaccat
    15241 tatctgtaac tatccgaacg gcgacatggt aggtcacacc ggggtgatgg aagcggcggt
    15301 taaagcggtt gaagcactgg atcactgcgt ggaagaagtc gcgaaagcgg ttgagtccgt
    15361 gggtggacaa ctgctgatca ccgctgacca cggtaacgct gaacagatgc gcgatccggc
    15421 aacgggtcag gcacacacgg cacacaccaa cctgccagtt ccgctgattt acgttggtga
    15481 taagaacgtg aaagcggttg caggcggcaa actttctgac atcgcgccga ccatgttgtc
    15541 gctgatgggt atggaaatcc cgcaagagat gactggtaag ccgctgttca tcgtggaata
    15601 atccctcccc atgaggggaa aggcgattaa taccatgaca cgggccgtga aaccgcgcag
    15661 gtttgcaatc aggcccatca tctacgccag cgttcttagc gctggcgtat tgttgtgcgc
    15721 cttttccgcc cacgcggatg agcgtgacca actcaaatct attcaggccg atatcgccgc
    15781 aaaagagcgc gcggtacgcc aaaagcaaca acaacgcgca agcctgctcg cacaattgaa
    15841 aaagcaggaa gaagcgatct ctgaagccac ccgtaagctg cgcgaaacgc aaaacacgct
    15901 caatcaactc aataaacaga ttgatgagat gaacgcgtcg attgccaaac tggagcagca
    15961 aaaagccgcc caggagcgca gcctcgccgc acaactggat gccgcattcc gtcagggcga
    16021 gcataccggt attcagctaa ttctcagcgg tgaagaaagc cagcgtggac agcgtttaca
    16081 ggcttatttc ggctatctca accaggcgcg acaagaaacc attgcccagt tgaagcaaac
    16141 gcgtgaagaa gtcaccatgc agcatgctga actggaagag aaacaaagcg agcaacaaac
    16201 gctgttatat gaacagcgcg cccaacaggc gaaactgact caggctctga acgagcgtaa
    16261 aaagacgctg gcggggctgg aatcttccat ccagcaaggt cagcagcagc taagtgaact
    16321 gcgtgccaac gaatcccgtc tgcgtaacag cattgcccgt gctgaagccg cggcgaaagc
    16381 gcgtgctgaa cgagaagcgc gtgaggccca ggcggttcgc gagcgccaga aagaagcgac
    16441 gcgcaaaggc accacctaca aaccgaccga aagcgaaaaa tcgctgatgt cccgaactgg
    16501 tggcctgggt gcgccgcgtg gtcaggcatt ctggccggtt cgcgggccga cgctgcatcg
    16561 ctatggcgag cagctacagg gtgaattacg ctggaaaggg atggttatcg gtgcctctga
    16621 aggtactgaa gttaaagcaa ttgccgacgg tcgagtgatt ctggctgact ggctgcaagg
    16681 ttacggtctg gtggtggtgg ttgagcacgg taaaggcgac atgagtcttt acggctataa
    16741 tcagagcgca ctggtgagcg ttggttcgca ggttcgcgcg ggccagccaa ttgcactggt
    16801 gggcagcagt ggcggtcagg gtcggccttc actctatttc gaaattcgcc gccagggtca
    16861 ggcggtcaat ccacagccgt ggttgggaag ataagttttg tttccatttc gtcgtaacgt
    16921 tcttgcattt gccgctctgt tggcgctctc ctcccccgta cttgctggca aacttgccat
    16981 cgtcattgat gattttgggt atcgcccgca caacgaaaac caggtgctgg cgatgccttc
    17041 cgctatctcc gtcgctgtat tacccgattc accgcacgcc agagagatgg cgaccaaagc
    17101 gcataacagc ggtcacgaag tgttgattca tctcccgatg gcaccgttga gtaaacagcc
    17161 gctggagaaa aatacgctac gcccggagat gagcagcgac gaaattgagc gcattattcg
    17221 tagtgcggtc aataacgtgc cctatgccgt ggggatcaac aaccacatgg gcagcaagat
    17281 gacctctaac ctgtttggta tgcagaaagt gatgcaggcg ctggagcgtt acaatcttta
    17341 cttcctcgac agcgtaacca tcggtaatac ccaggcgatg cgcgccgcgc aaggtactgg
    17401 cgtgaaggtg atcaaacgga aggtgttcct cgacgattcg cagaacgaag cggacatccg
    17461 tgtgcaattt aatcgcgcaa ttgatctggc gcgtcgcaac ggttcaacca ttgccattgg
    17521 gcatcctcac ccttcaacgg tacgcgtgct acaacagatg gtttataacc tgccgccaga
    17581 cattacgctg gtgaaagcca gcagcttgct gaatgaaccg caggttgata cttctacacc
    17641 gccgaaaaac gctgtgcctg atgcaccgcg taatccgttc cgtggcgtga agctgtgcaa
    17701 accgaagaaa ccgatagaac ctgtttatgc taatcgcttc tttgaagtat taagcgaaag
    17761 catcagccag agcacgctga tcgtttactt ccagcatcag tggcaaggct ggggcaaaca
    17821 gcccgaagcg gcgaagttta acgctagcgc aaattaaagg cgctacgcgt aatgcgatgg
    17881 tgcgaccgcg ttttgtcgcg ccattgccat aagcggaaag accacagtaa cgcctggtaa
    17941 ccgaccttca cgctgcgcac attggtaatc aggcgcttat acatacccgg aagtaaatat
    18001 ctcggcaatc atccgttggc gggtaagaat atccggctct ttgcg
//
LOCUS       DADLTY010000082        17644 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.082, whole genome shotgun sequence.
ACCESSION   DADLTY010000082 DADLTY010000000
VERSION     DADLTY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.082"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(1..671)
                     /gene="chbR"
                     /locus_tag="JN334_002334"
                     /pseudo
     CDS             complement(1..671)
                     /gene="chbR"
                     /locus_tag="JN334_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="JN334_002335"
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="JN334_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HAY7438455.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="JN334_002336"
                     /pseudo
     CDS             complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="JN334_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="JN334_002337"
     CDS             complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="JN334_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HAY7438456.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="JN334_002338"
     CDS             complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="JN334_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HAY7438457.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3680..4507
                     /gene="nadE"
                     /locus_tag="JN334_002339"
     CDS             3680..4507
                     /gene="nadE"
                     /locus_tag="JN334_002339"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAY7438458.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4737..5624
                     /gene="cho"
                     /locus_tag="JN334_002340"
     CDS             4737..5624
                     /gene="cho"
                     /locus_tag="JN334_002340"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HAY7438459.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5584..6159)
                     /gene="ves"
                     /locus_tag="JN334_002341"
     CDS             complement(5584..6159)
                     /gene="ves"
                     /locus_tag="JN334_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HAY7438460.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6362..6847)
                     /gene="spy"
                     /locus_tag="JN334_002342"
     CDS             complement(6362..6847)
                     /gene="spy"
                     /locus_tag="JN334_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HAY7438461.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7177..8145)
                     /gene="astE"
                     /locus_tag="JN334_002343"
     CDS             complement(7177..8145)
                     /gene="astE"
                     /locus_tag="JN334_002343"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HAY7438462.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8138..9471)
                     /gene="astB"
                     /locus_tag="JN334_002344"
                     /pseudo
     CDS             complement(8138..9471)
                     /gene="astB"
                     /locus_tag="JN334_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9468..10946)
                     /gene="astD"
                     /locus_tag="JN334_002345"
     CDS             complement(9468..10946)
                     /gene="astD"
                     /locus_tag="JN334_002345"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HAY7438463.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10943..11977)
                     /gene="astA"
                     /locus_tag="JN334_002346"
     CDS             complement(10943..11977)
                     /gene="astA"
                     /locus_tag="JN334_002346"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HAY7438464.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11974..13194)
                     /gene="astC"
                     /locus_tag="JN334_002347"
                     /pseudo
     CDS             complement(11974..13194)
                     /gene="astC"
                     /locus_tag="JN334_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13640..14446
                     /gene="xthA"
                     /locus_tag="JN334_002348"
     CDS             13640..14446
                     /gene="xthA"
                     /locus_tag="JN334_002348"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAY7438465.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14613..15323
                     /locus_tag="JN334_002349"
     CDS             14613..15323
                     /locus_tag="JN334_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY7438466.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15328..16005
                     /gene="ydjY"
                     /locus_tag="JN334_002350"
     CDS             15328..16005
                     /gene="ydjY"
                     /locus_tag="JN334_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HAY7438467.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            16020..16727
                     /locus_tag="JN334_002351"
     CDS             16020..16727
                     /locus_tag="JN334_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY7438468.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16727..17275
                     /locus_tag="JN334_002352"
     CDS             16727..17275
                     /locus_tag="JN334_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAY7438469.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17285..17611
                     /locus_tag="JN334_002353"
                     /pseudo
     CDS             17285..17611
                     /locus_tag="JN334_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         4366 a         4686 c         4307 g         4285 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactacg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatactc tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaac gacccctccc caaagcttag
     2281 aaaaacgttg ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat
     2341 aaagccattt ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag
     2401 gagtaccttt tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca
     2461 atttaaatat tacggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa
     2521 ttaatttgct gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc
     2581 gactttgcca taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg
     2641 gatttcgggc aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc
     2701 tttttcacca gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc
     2761 tgcctgtgcg cgcatttttg ataccagtaa agagatagac atgcccgcag aacaaaacag
     2821 ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac
     2881 tccgcaagcc ttgacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt
     2941 ttgtggctga aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa
     3001 attgtttctc cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga
     3061 agataattaa gaactcaggc caaaaaaaac cagcgcaatg gccggtttcc gttgttactc
     3121 aagctttcag acgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg
     3181 tctgataacc ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg
     3241 ctttaccatc acgttgaccc aggatgtagg tctggcaagt accgcgagca tggatcatgc
     3301 tcacttcaga cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt
     3361 tgacgtcttt caccacaggc tgtacaaact ggtctttggt acgatcataa gctgtacaac
     3421 ccgccagcat ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat
     3481 tccgtcctct tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa
     3541 gcccgtgaac gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt
     3601 cagcccgctg tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt
     3661 ttctgtctgg aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa
     3721 accgcagatt aatgctgaag aggaaattcg tcgtagtgtc gattttctga aaagctacct
     3781 gcaaacttat ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac
     3841 gcttgccgga aagctgtgcc agatggcgat taatgagctg cgccaggaaa ctcgcaacga
     3901 atcactgcaa tttattgccg tacgcctgcc ctatggtgtt caggctgacg aacaagattg
     3961 ccaggatgcc attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc
     4021 ggtattggct agcgagcagg cattgcggga agcaggcatt gaactgagcg attttgtccg
     4081 tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag
     4141 cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata
     4201 tggtgacggc ggtacggata ttaatccgct gtatcgtctc aacaaacgcc agggtaaaca
     4261 gttactgacg gcattaggtt gcccggaaca cctttataag aaagccccaa ctgccgatct
     4321 tgaagatgat cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat
     4381 cgacgactat ctggaaggga aaaacctacc tgaacaggtc gccagaacaa tagagaactg
     4441 gtatctgaaa accgaacata aacgccgtcc gccaattaac gttttcgatg atttctggaa
     4501 aaagtaataa tttgcaccta ttggattgtg cgaaaaagag taatttgttc acgccggatg
     4561 cggcgtgaac gccttattct acctacaaaa ctatgcaaat tcaatacatt gcaggagtcg
     4621 aataggcctg acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa cccgcctctt
     4681 ttttaatcgc ctcccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg
     4741 tacggcgttt aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg
     4801 aacatttaca ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg
     4861 gcgaaagtga caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc
     4921 tttctcattt acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct
     4981 ggatctgcac cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac
     5041 aacagccgct gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa
     5101 atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc
     5161 tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc attgcagagc atcgctgatg
     5221 aacaaaaact ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt cgtgcatgtt
     5281 ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac
     5341 atgcgctacg cttgcgccag tctctggagc gtttgcgagt ggtgtgttgg ccttggcaag
     5401 gtgcggtggc actgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact
     5461 ggctgtggct gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg
     5521 ccgggtttga tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg
     5581 aaattactga acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag
     5641 tttaccttgc ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acacgccctg
     5701 gtcggtagtg agcagtttgt cgcccagttg ccacgccccg ttaatcacaa acaccacccc
     5761 gccgcgtgag ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac
     5821 atccaggcgc gtcataatat tgaaatccat cgacatctgc cctgccgtca gtttcgcttt
     5881 taccacctgg tccgccgtaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg
     5941 gtctgcgctt tcaaggagca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc
     6001 gggaaacaaa gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa
     6061 atcacgtttt gccggtggga aagtgcaaat ttcgcgcgtt tcaccggcag cgtttcgcca
     6121 cagattcacc gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt
     6181 ttcattgtta tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta
     6241 gtggtgatca ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt
     6301 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag
     6361 attattcagc agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct
     6421 caaaattagc attaaattgc tttttctgtt ccggcgtcag gatgttgtaa attttgttct
     6481 gggtttccat gtgcgccagc atgttagctt tgcgctgttc ttccattttt gcgatctgcg
     6541 cttcagcttt tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcggcgtt
     6601 cttccagcgg cggacgtttc atctggtcac gctggccttt catgatttcg tggatctgct
     6661 gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact
     6721 tgcctttgtg gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg
     6781 catgggccag gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt
     6841 tacgcatatt ctatatcctt cctttcagtt atttattacg gctttcttaa gtagcgtgcc
     6901 gtgttgacga gattaacttt actgacttta gcgtcaatta atcagagcaa cggtaaaaca
     6961 atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa tttgatggag
     7021 agtgatgaga gaatattaca acacgatgat tttgcagaga ttatgaagaa ctataccgga
     7081 tgactggtga taaataaagc aaataaccag gattaatctg tattaattta taagaaagca
     7141 acttaatacc cgcagaatga tttctgcggg taagtattag cttatttttt cgagcattaa
     7201 tcccgcgcgt aatcccaacg ctaccaacgg attagggaat aacacatact ctacatcatg
     7261 ggttacggta aaacgttcct ctccgtcctg cgccagcaat gttcctttct caaacggcat
     7321 aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc gagtaatttg
     7381 cgaaaccacc cgataacgga gcggcggtgt tctcacgata ccgacactct caccagatag
     7441 cagcgcagca attgcgctgg cagttactgc aaactggcga agatcgtttt gcccaaaggg
     7501 caacgctttg ccaagttcca acgtacaggc cagcgcgcca aaatgtctga cgctgaaatg
     7561 ggtaaacgta ccaccaggct cctgatggaa caccagcgcc tccagccccg ccgcacccag
     7621 ccacgtcaga aatttctcgt cccagggaat gtcgcgttgc ggtaatacac cgaactgcgg
     7681 atgcaaggag ccacgaattg cggtatgtag atcaaggtgc caacgcacag attctttgcc
     7741 ctggtcataa aaatcttcca ggcactgttc cagttcgcgc gcccgacagg tttctccgct
     7801 ttcagcaaat agctgccaac gaccgccaaa cattcggttc atatcgctat ggcaataacg
     7861 tttcccttgc ttcagcgcag gaggattccc gaggatcacc agcaaccgcc aacgtaacgg
     7921 gatctcgccg tgagatatcg cgccaagcaa cgcgtccagc atttccacag gtgccgtctc
     7981 attaccgtgg atccccgcag aaatcaccag cgcgccttgc ggtggcgtta atggcgtcag
     8041 ttccagcaca ccatcgccca gccagcgcca gcgaacgccg ttgatttctc gctcggtgat
     8101 aaccggtttt ttacccgtta aggtcagagc aagaaaatta tccattgccc cctccctcgc
     8161 gctggaacgg ataaaccgaa ccgagattca gtaattggct caatacatcc agtgcttccc
     8221 gcccttcgcg cagcaattgc gggtcggcca gatcggcagc agtaagacga tcgcggtagt
     8281 aacgatccac ccagtcattg agcgcattaa acaaggtatc gttcatcatc accgccggat
     8341 tcaccgcccg gcgttcttct tctgtcaata ccacccgcaa ccgcaggcac gccgggccgc
     8401 cgccattcgc catgctttca cgtaagtcaa agacttttag ttcgctaatc gggttgtcag
     8461 cggcaaggag ttcattgaga taaccccata ctccggcgtg ttcccgacac tcctgaggca
     8521 gcaccaacat catggaacca tcatcgcggc tcaacagttg gctgttaaac agataggtag
     8581 acaccgcatc cgacacggaa acctgagttg ccggaacttc tatcgccata aaaccgttca
     8641 cccgcgtgcc agtaactgtg actggcgagc gaacgcctgt tggtggcaaa acagcacctg
     8701 gcggttactc acggcaatca cgtcattatg aaaaacgctc tggtcgataa cgtccgggtt
     8761 ttgctgggcg aaaatcacct gttggggatt cacctgatta agccttgcca ccgcctcgct
     8821 ggcttcgcga gtctgtcgcg ccggataacg ggaaggccgg gtatcattgc cttcttctcg
     8881 cccgtagaca aaaagtagca tacccggttc accgtaatga ccgccgagac gattgtggtt
     8941 tgccgccccc tcatcaccga gcaacgctac ctgcggcaac gccgaatgga cgctaaattt
     9001 ctcttcgtcc ttaaaaatcg cttttaacag cgattcagtg acgggcgctt ccagcgaacg
     9061 gtgaaattta ttgttcaggt tggcaacggt gagatgcact ttgccatcca gcgtatcggc
     9121 agatggcgcg atcgttgccg cattggctac ccacattggc gaagcggaac tgacgctgga
     9181 aagccagtgc ggtgcctggc gtgcaacttt ttccagtacc tgctcatcgc taccactgaa
     9241 tcccaactga cgcagcaccg gaataaacgg acgctcgtgc ggcggaatca cggcctgggg
     9301 gaatcccgca tcggcaaggg ctttcatttt cagtaagccc tgttttgccg ccagtcgcgg
     9361 gttagacacc tgaaaacggt gacgggtaga ggcttcatta ccaaacgaca ggcccgcgta
     9421 atgatgcgtc agccctacca gcccgtcgaa attaacttcc caggcgttca tcgcaccacc
     9481 tcatcggaaa aatccagccc ggggttaagc gtggcgggca atgttaacga gtccgactcc
     9541 aggctcgcca taggccatgc gcagtaatct gcggcatacc aggcgctggg gcgatggtta
     9601 ccggaagcac caatgccgcc gaatggggcg gtactggcag caccagtaag cggtttgttc
     9661 cagttaacaa tccccgcccg cgcctccagc aacagttgat cgaatttttc ccgctcgggg
     9721 gaaaccagac cgcaaaagag tccgaagcga gtgttgttcg ccatccgaat cgcctcatca
     9781 aaagtatcat aacgccagac gcgcaaaaac ggcccgaaca cctcttcatc cggcacaccg
     9841 ccaacgcccg tcatttcaat gatccctggt gtcagcaacg atgtccctgc ttgtaataag
     9901 cgcggcgcaa gcagggttcg tccgcccatc gcttccagtt gctgccaggc agtaaccacc
     9961 tgctgtgcgg cctgttcaga aatcagcccg ccaataaacg gctgcggttc gtcatcccag
    10021 ttgcccggcg ttaatcgctg gctgacggca accagactag caagaaacgc atcgccctgc
    10081 gctccgcttt tgagcaataa acggcgggcg caggtgcagc gttgcccggc tgtgacaaac
    10141 gccgactgaa tggtcagatg gacagccgcg tcgatatccg ccacctcatc gataattagc
    10201 ggattattac cgcccatctc aagggcgaga attttctccg gctgaccgga gagctggcga
    10261 tgcagctggt agccggtatt ggcgctaccg gtaaacagca aaccgtcgag atcctccagc
    10321 gcactcagcg cctgacccgt ttcacgcccg ccctgcacca ggttcagcac gcccggcggc
    10381 aagccagcct gctgccataa acgcattacc gcttcgccac tccacggtgt cagttcgctg
    10441 ggtttaaaga taatggtgtt acctgccagc aatgccggaa cgatatgtcc gttcggcaaa
    10501 tgaccaggga aattatacgg cccaaacacc gccagcacgc cgtgcgggcg atgtcgcagg
    10561 ctcgccgcac cgtccggcat ttcactacgc tgctcgccgg tacgaacgtg atacgcctta
    10621 attgatatcg cgattttatt gatcatcgcc gtcacttcgg ttgccgcttc ccagcgcggc
    10681 ttacccgttt ctctggcaat aatcgcggtt aattcggctt tattgctttc cagcaatccg
    10741 gcaaagcgtt cgacaacgac ctgacgttca gccaatgaga gccgcgccca gcgcggaaac
    10801 gccgcacggg ctgcccgaca agcctgttcg acctgagcgg catcggcatc attgccttgc
    10861 cataacacct cgcccgatac cggattacgc ttcacacgcg atgcgccctg gcccgttatc
    10921 cagtcaccgt taatccataa agtcatgctg ttttctcctc tgcgcacagg cgcaccagac
    10981 gaacgcgatc ccctgcgtgg catttgaggg catccagttg tgcggcggtt aaaatcaaac
    11041 gctcggttgc cggatcggta cgcgccagca ccacgcggaa atggtgataa ttttcattgg
    11101 cgaccaggca ggccggaaaa tcgccctgcg caggctgccc ttctgccact tccaccagcc
    11161 gacttttacg gatggcgcgc acgcggtcga tgtcacactc aagcgttggc ccgccgtcga
    11221 agatgtcgat atagttgcgg taacgaaaac cttctttctc cagcaccgcg cgggcaggcg
    11281 cggtttgcgg atgtacctga ccgatgacgt cctgggcttc ctgggataaa aagtgggtat
    11341 agatcggatg tttcggcatc agttctgcaa taaatgcctt ttgcccggtg ccgcagagaa
    11401 aatcggcgcg gctaaaatcc atcgaaaaga agcgtttacc gaggctttgc cagaacggtg
    11461 aatagccgtg ttcgtcaatc accccgcgca tttcagcaac cactttgtca ttaaacttgt
    11521 cgcgaaaagc cgccataaac ataaagcgcg atttcgacag caaatagccg ttgccctctt
    11581 tgcgccagtc cgggtcgaga aacagcgtgc acagctcgct gctgccggtg tgatcgttac
    11641 taagaaacag cgtcggcaat gcgttataga cattcagctc ttttgaggcg tgaaccaacg
    11701 tgccgacgcg atagttgtac cagggatcgt tcagcccaac cgccacctca atagcgcaaa
    11761 tccccgccac ggtgcctgtc tcgctatctt ccagcacgaa cacatagccc tgctcgcttt
    11821 tgggcagttc accttgccag gttttgattg ccctttcgat acgcgccgaa agcgtggctt
    11881 cattggcggg aagcgacgtc aggccgccgc ccgttttgct ggcaagctgc atcagcgccg
    11941 agacatctga gcgctcaacg ggacggatga ccatcatgat gaacctcggc taacaaagtg
    12001 ttcgcaagca gctgcaaagc gatccagtcc ggtcgtcacc tcttcttcgc tgacattgag
    12061 cgcaggcgca aaacgcacca cgttgccacc cgcaatcagt accatcacgc ctgctttcgc
    12121 cgcttccaga gagatctgtt tcgcttgccc ggcgtaatcg gcattcagta cacagccaat
    12181 caacaaacct aagccgcgaa cttcactgaa caaaccatag cggtgattaa tagtattaag
    12241 acgctcaaca aaccagtcgt gacgctgttt aacgccatta agcatctctg gtgtgttgat
    12301 gagctccagc actttgcctg ctaccgccga ggccagcgga ttaccgccat aggtggtgcc
    12361 atgagtgcca acggtcatca cgcgggcgca ctcttcggtt gccaacaacg caccgaccgg
    12421 gaaaccgccg cccagcgctt tggcggtggt taacagatcg ggcgtcacgc cgtagtgcat
    12481 ataggcatac agctcaccag tgcgcccgac gccggtttgt acctcatcaa aaatcagcaa
    12541 ggcattgtgg cggtcacaca attcacgcag accttgtaaa aacgcgttgc tggctggcac
    12601 cacaccgcct tcgccctaga tgggctcgac aatcaccgca caggtggcgt cgtcaatcag
    12661 cgcgctggca gagttaatat cgttatatgc agcatgacga atatccgccg gcagtggcgc
    12721 aaaatcctgt gaataggctg gctgcccacc cgcactgaca gtaaacagcg tgcgaccatg
    12781 aaacgcattt ttgaacgcca cgatgccact cttatggctg ccgtagcggt cgtgagcgaa
    12841 tttacgcgcc agttttagcg ccgcttcgtt ggcttccgca ccggagttac aaaagaagac
    12901 gcgatcggca aacgtggcgt cgatcaattt tttcgccagt cgcagtaccg gctcgttggt
    12961 gtaaccgttg ccggtatgcc agaacttact cgcctgttcg ttcagcgctt cacgcagttc
    13021 cggatgcgca tggcccagcg cgttcaccgc aatgccaccc gcgaagtcga tatactcttt
    13081 cccctgctga tcccacaagc gcgaaccttc gccacgtacc ggtataaagg gtgccggagc
    13141 gtaaacaggt atcatccatt catcaaagtt ttcacgcgta attggctgag acatagcgac
    13201 ctctacagta aataattcgt tatttgtatg ttaacaataa gtaatgtttg cgctgtaaat
    13261 gtagattgca gggttcgtgc cagccagtga taaaagtgca taaacggcgg aggctaactg
    13321 gaaatcaagg agttataacc aaaccatatg catttaaagt gcatataaag tgaatacgtt
    13381 tgcgatgtgg gtgaataaaa agaataaaaa acgcaatgtt atgcagaagt aaaatataat
    13441 tctagaattg tgatcattga cgaaatttac tggaaattac tgcgccattc tgacgcagcg
    13501 cgcaccaaaa gcgggcattt tttgcgccat cgttgacatc attaacaacc atcgatcaaa
    13561 tcacttaaca acaggcggta agcaacgcga aattctgcta ccatccacgc actctttatc
    13621 tgaataaatg gcagcgacta tgaaatttgt ctcttttaat atcaacggcc tgcgcgccag
    13681 acctcaccag cttgaagcca tcgtcgaaaa gcaccaaccg gatgtgattg gcctgcagga
    13741 gacaaaagtt catgacgata tgtttccgct cgaagaggtg gcgaagctcg gctacaacgt
    13801 gttttatcac gggcagaaag gccattatgg cgtggcgctg ctgaccaaag agacgccgat
    13861 tgccgtgcgt cgcggctttc ccggtgacga cgaagaggcg cagcggcgga ttattatggc
    13921 ggaaatcccc tcactgctgg gtaatgtcac cgtgatcaac ggttacttcc cgcagggtga
    13981 aagccgcgac catccgataa aattcccggc aaaagcgcag ttttatcaga atctgcaaaa
    14041 ctacctggaa accgaactca aacgtgataa tccggtactg attatgggcg atatgaatat
    14101 cagccctaca gatctggata tcggcattgg cgaagaaaac cgtaagcgct ggctgcgtac
    14161 cggtaaatgc tctttcctgc cggaagagcg cgaatggatg gacaggctga tgagctgggg
    14221 ggtggtcgat accttccgcc atgctaatcc gcaaacagca gatcgtttct catggtttga
    14281 ttaccgctca aaaggttttg acgataaccg tggtctgcgc atcgacctgc tgctcgccag
    14341 ccaaccgctg gcagaatgtt gcgtagaaac cggcatcgac tatgaaattc gcagcatgga
    14401 aaaaccgtcc gatcacgccc ccgtctgggc gaccttccgc cgctaattta gcagctctcc
    14461 tggctcaaac tgggtcagga gaattaacct tgagaaaaat caacaaactg tcagtaatga
    14521 tttgttgcct gccgtccttt gttataccgt cactgcgttt ttagttgtct gaccacttct
    14581 ctattatcaa gtttgatata ggaaactcca cgatgaacgc tgagcgtaac tttctgtttg
    14641 cctgtcttat ttttgcgctg gtcatttacg ctatccacgc tttcggttta ttcgatctgc
    14701 tcaccgattt accccactta cagacactca ttcgccagag cggattgttc ggttatagcc
    14761 tctatattct gttattcatc aatgcccccc tctttctgtt accaggaagc atactggtga
    14821 tcgctggagg aatagttttt ggcccgctct tagggacaca actctcatta attgccgcaa
    14881 cgctggcctc ctcgtgctca ttcctgctgg cgcgctggct gggacgtgat ttactgctga
    14941 aatacgtggg ccatagccat accttccagg ccattgaaaa aggcattgcg cgtaacggta
    15001 ttgattttct tattctgacc cgcttaatcc cgttgtttcc ttacaatatt caaaattacg
    15061 cttacggatt caccaccatc gccttctggc cttataccct tatttcggca ctcacgaccc
    15121 tgcccggtat tgttatttat accgtgatgg caagcgatct cgccaatgaa ggcattacgc
    15181 tgcgctttat tttacaactc tgtctggcgg gcctggcgct gtttattctc atccagctcg
    15241 caaaactcta cgcccgacac aaacatgtgg atctgtctgc ttcgcgccgc agcccactta
    15301 ctcacccaaa aaatgaagga tagaacgatg tcgcaacatt attcagtgtc atggaaaaaa
    15361 ggactggctg cactctgctt actggctgtt gccgggctta gcggctgcga tcaaaaagag
    15421 aatgcggcgg caaaagtgga atacgacgga ctttcgaaca gccaaccgct gcgcgtcgat
    15481 gccaataacc atacggtaac catgctggtg caaattaatg gtcgtttcct caccgacgac
    15541 actcgtcacg gtattgtgtt taaagatggc tccaacggac ataaatcgct gtttatggct
    15601 tatgcgcccc cgaaagcatt ttatgaagcc ctgaaagagg caggtggtac gccgggcgaa
    15661 aacatgacga tggataataa agaaacgact catgtcacag gcagcaaact ggatatttcg
    15721 gtcaactggc aaggggcggc aaaagcgtat tccttcgatg aagtgattgt tgacagtaat
    15781 ggcaagaaac tggacatgcg ctttggcggt aatttaacgg cagcagaaga gaagaaaacg
    15841 ggttgcctgg tgtgtctgga tagctgcccg gtcggcatcg tcagcaatgc aacatacact
    15901 tatggtgcgg tggaaaaaca cggcgaagtg aaattcaaag gcaatgcctc agttctcccg
    15961 gcggataaca cgctggcaac ggttaccttt aaaatcaccg aataaagcca agataaagga
    16021 tgatgaagat acaatcgcgt aaaatctggt actaccgtat aaccctcatc atcctgttgt
    16081 tcgctatgct gctcgcatgg gcgctgcttc ccggcgtcca tgagtttatc aatcgcagcg
    16141 ttgcggcgtt tgccgccgtg gaccaacagg gtatagaacg ctttattcag tcttacggcg
    16201 cactggcggc agttgtctcg ttcttgttga tgattttaca ggccattgct gcaccgctac
    16261 ctgcgttttt gatcaccttt gccaatgcgt cgctgtttgg cgcgttctgg ggcggcttgc
    16321 tgtcgtggac cagttcgatg gccggcgcgg cgctgtgctt ttttatcgcc agagtgatgg
    16381 gccgcgaagt ggtggaaaaa ttaaccggca aaaccgtgct tgacagtatg gacggctttt
    16441 tcactcgcta cggcaaacac accatcctgg tctgtcggtt attgcctttt gtccctttcg
    16501 atccaatcag ctatgctgcc ggtctgactt caatacgttt tcgctcgttt tttatcgcca
    16561 ccgggcttgg tcaattaccg gcgactattg tttattcctg ggcaggcagc atgttaacag
    16621 gcggtacttt ctggtttgtc accgaactgt ttattctgtt tgccctgacc gtggtgattt
    16681 ttatggcgaa gaaaatatgg cttgagcgcc agaagaggaa tgactgatgg gtttaccgcc
    16741 gcttagcaaa attcctttca ttttacgtcc gcaggcgtgg ctgcatcgtc gccattacgg
    16801 cgaggtgcta agcccgattc gctggtgggg gcggatcccg tttatctttt atttggtgtc
    16861 aatgtttgtt gggtggctgg agcgcaaacg ctcaccgctc gatccggtag tgcgatcgct
    16921 tgtcagcgcg cgcattgcgc aaatgtgcct gtgtgagttt tgcgtggata tcaccagtat
    16981 gaaagtcgcc gagcgtaccg gcagcaccga taaactgctg gcagtggctg actggcggca
    17041 aagcccgctc tttagcgatg aagaacggct ggcgctggag tacgccgaag ccgcaagcgt
    17101 cacaccgcca acagtcgatg atgccctgcg tacccgactg gctgcgcatt ttgacgctca
    17161 ggcgctcacc gaactgacgg cattgatcgg cctgcaaaac ctgtcggctc gttttaattc
    17221 tgccatggac attcccgctc aggggctgtg ccgtattccg gaaaaacgtt cttaaggagg
    17281 gaagatgcgc cattgtgggt ggttgctggg attgttatcg ctgttttctt tggcaacaca
    17341 tgccagtgac tggcaggaaa ttaaaaatga ggccaaaggg caaaccgtct ggtttaacgc
    17401 ctggggcggc gataccgcaa ttaaccgcta tctcgactgg gttagcggcg agatgaaaac
    17461 ccattacgct ataaacctga agattgttcg tctcgcggat gccgcagacg cggtgaagcg
    17521 cattcagacc gaagctgctg ccggtcgtaa aacgggcggc tcggtggatc tgctctgggt
    17581 gaacggcgaa aacttccgca ccttaaaaga gtaatgccag tcagttaagc aactgactgg
    17641 catt
//
LOCUS       DADLTY010000083        17624 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.083, whole genome shotgun sequence.
ACCESSION   DADLTY010000083 DADLTY010000000
VERSION     DADLTY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.083"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(11..322)
                     /locus_tag="JN334_002354"
     CDS             complement(11..322)
                     /locus_tag="JN334_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HAY7438470.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="JN334_002355"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JN334_002355"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="JN334_002356"
     CDS             701..1054
                     /locus_tag="JN334_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HAY7438471.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="JN334_002357"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="JN334_002357"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HAY7438472.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="JN334_002358"
     CDS             complement(2810..3280)
                     /locus_tag="JN334_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438473.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="JN334_002359"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="JN334_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="JN334_002360"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="JN334_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY7438474.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="JN334_002361"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="JN334_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HAY7438475.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="JN334_002362"
     CDS             complement(5283..8432)
                     /locus_tag="JN334_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAY7438476.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="JN334_002363"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="JN334_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HAY7438477.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="JN334_002364"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="JN334_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HAY7438478.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="JN334_002365"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="JN334_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HAY7438479.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="JN334_002366"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="JN334_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HAY7438480.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="JN334_002367"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="JN334_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HAY7438481.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="JN334_002368"
     CDS             14911..15288
                     /locus_tag="JN334_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HAY7438482.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="JN334_002369"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="JN334_002369"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAY7438483.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..>17624
                     /gene="dnaX"
                     /locus_tag="JN334_002370"
     CDS             16121..>17624
                     /gene="dnaX"
                     /locus_tag="JN334_002370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAY7438484.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVLPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEH"
BASE COUNT         4334 a         4504 c         4488 g         4298 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctctcg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcacagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacctgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgctg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catg
//
LOCUS       DADLTY010000084        17410 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.084, whole genome shotgun sequence.
ACCESSION   DADLTY010000084 DADLTY010000000
VERSION     DADLTY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.084"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            131..574
                     /locus_tag="JN334_002371"
     CDS             131..574
                     /locus_tag="JN334_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7438485.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            810..1628
                     /locus_tag="JN334_002372"
     CDS             810..1628
                     /locus_tag="JN334_002372"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HAY7438486.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1629..2687)
                     /gene="modC"
                     /locus_tag="JN334_002373"
     CDS             complement(1629..2687)
                     /gene="modC"
                     /locus_tag="JN334_002373"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HAY7438487.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2690..3379)
                     /gene="modB"
                     /locus_tag="JN334_002374"
     CDS             complement(2690..3379)
                     /gene="modB"
                     /locus_tag="JN334_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAY7438488.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3379..4152)
                     /gene="modA"
                     /locus_tag="JN334_002375"
     CDS             complement(3379..4152)
                     /gene="modA"
                     /locus_tag="JN334_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7438489.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4319..4468)
                     /locus_tag="JN334_002376"
     CDS             complement(4319..4468)
                     /locus_tag="JN334_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HAY7438490.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4597..5385
                     /gene="modE"
                     /locus_tag="JN334_002377"
     CDS             4597..5385
                     /gene="modE"
                     /locus_tag="JN334_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HAY7438491.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5453..6925
                     /gene="modF"
                     /locus_tag="JN334_002378"
     CDS             5453..6925
                     /gene="modF"
                     /locus_tag="JN334_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HAY7438492.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7186..8202
                     /gene="galE"
                     /locus_tag="JN334_002379"
     CDS             7186..8202
                     /gene="galE"
                     /locus_tag="JN334_002379"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAY7438493.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8212..9258
                     /gene="galT"
                     /locus_tag="JN334_002380"
     CDS             8212..9258
                     /gene="galT"
                     /locus_tag="JN334_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAY7438494.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9262..10410
                     /gene="galK"
                     /locus_tag="JN334_002381"
     CDS             9262..10410
                     /gene="galK"
                     /locus_tag="JN334_002381"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAY7438495.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10404..11444
                     /gene="galM"
                     /locus_tag="JN334_002382"
     CDS             10404..11444
                     /gene="galM"
                     /locus_tag="JN334_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAY7438496.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11647..12399
                     /gene="gpmA"
                     /locus_tag="JN334_002383"
     CDS             11647..12399
                     /gene="gpmA"
                     /locus_tag="JN334_002383"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAY7438497.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLRRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="JN334_002384"
     CDS             complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="JN334_002384"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HAY7438498.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13934..14314
                     /locus_tag="JN334_002385"
     CDS             13934..14314
                     /locus_tag="JN334_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438499.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14428..15369
                     /gene="zitB"
                     /locus_tag="JN334_002386"
     CDS             14428..15369
                     /gene="zitB"
                     /locus_tag="JN334_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HAY7438500.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15366..16085)
                     /locus_tag="JN334_002387"
     CDS             complement(15366..16085)
                     /locus_tag="JN334_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HAY7438501.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="JN334_002388"
     CDS             complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="JN334_002388"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAY7438502.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         4439 a         4543 c         4326 g         4102 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      361 gacaattgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      421 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      481 cagtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      541 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaatg
      601 ccacttgtaa ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg
      661 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      721 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      781 aaattcttaa ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag
      841 acggcacctt attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc
      901 gcgctcgcga agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc
      961 atccttttta tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt
     1021 tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc
     1081 tgcaactcat tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg
     1141 caatggtcta tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc
     1201 tgccgccgga acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac
     1261 aagttaacgc cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt
     1321 ttggtaagca tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg
     1381 ttgatattgc acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc
     1441 aaggttggtc gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc
     1501 tggaagccgc tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg
     1561 ccaacattgt gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc
     1621 tgatttaatc aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt
     1681 ttgatcgcca gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg
     1741 acttccagct ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc
     1801 aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc
     1861 tgaatgcgga tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc
     1921 tgatcgccca gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact
     1981 ttcagaatgc tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac
     2041 acttcctcca gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct
     2101 gccagatgga ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc
     2161 cgtgtcagcc gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc
     2221 agcggttcat ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg
     2281 cgctgttttt cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct
     2341 aaaagcgcca ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga
     2401 ttgccacgca ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga
     2461 cgcttttccg gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg
     2521 acaatccgcc ctttttgcgg gcgagtcagt ccactgatgg cgttaatcag cgaagttttt
     2581 ccggcaccgg agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg
     2641 gtcaggcaat ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg
     2701 cccgttcacg gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg
     2761 caatagaaat aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg
     2821 tatacatggc agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg
     2881 ttgcaccaaa ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg
     2941 gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc
     3001 ttgcggcctg ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca
     3061 tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg
     3121 taataccaaa ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta
     3181 ataataagta accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga
     3241 gcagagcttt gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg
     3301 gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt
     3361 ctggatcggt cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc
     3421 ctgcggtccc ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac
     3481 aacagcaacc ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt
     3541 tacccctttg ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg
     3601 ttcgaccagc gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt
     3661 atcccatgcg cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc
     3721 cggatcgcca accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc
     3781 gatggtgaaa tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag
     3841 cagtgtctga cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt
     3901 ctgatcggca gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt
     3961 agatgacgaa gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc
     4021 aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc
     4081 tgccagtgca ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca
     4141 tttacgagcc attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa
     4201 ctcggaattt tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg
     4261 caggaaaagc aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc
     4321 aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac
     4381 caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga
     4441 ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc
     4501 taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt
     4561 tttccgtcac aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc
     4621 cttaagctcc aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt
     4681 gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc
     4741 tgggatgcca ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca
     4801 ggcggtaaag gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc
     4861 tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc
     4921 ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt
     4981 aaccagtggt tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat
     5041 gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca
     5101 cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc
     5161 attactcagg acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt
     5221 catattgagc gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca
     5281 ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg
     5341 gcctacttta atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt
     5401 gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc
     5461 gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca
     5521 gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc
     5581 ggcgctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca
     5641 gttttcccac atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg
     5701 gcaacggaat aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc
     5761 tgagattatt caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt
     5821 cggtattacc gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa
     5881 aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt
     5941 cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca
     6001 gtccggtatt actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca
     6061 gtttgctggc gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct
     6121 ccagcaagca ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc
     6181 ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct
     6241 gaacaatggc gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt
     6301 gaatccaggc gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt
     6361 aagcctgatt actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg
     6421 acgtcgcggc agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag
     6481 tagtttgcat ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta
     6541 ttttgattcg attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca
     6601 gtggctggat attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc
     6661 ctggggacag cagcgtttgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct
     6721 tattctcgat gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt
     6781 tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga
     6841 agatgcgcct gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg
     6901 ctatgtgctg acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag
     6961 cgcactctcc ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata
     7021 aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg
     7081 gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt
     7141 gttatgctat ggttatttca taccataagc ctaatggagc gaattatgag agttctggtt
     7201 accggtggta gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat
     7261 gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag
     7321 cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg
     7381 accgagatcc tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc
     7441 gtcgccgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg
     7501 cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc
     7561 accgtttatg gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg
     7621 caaagccctt atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa
     7681 gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg
     7741 tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc
     7801 caagttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa
     7861 gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg
     7921 gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta
     7981 ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac
     8041 tatcattttg caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa
     8101 gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc
     8161 tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa
     8221 tttaatcccg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg
     8281 gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag
     8341 gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc
     8401 gataaaaacc ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg
     8461 tctgacacgc cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc
     8521 ggtaccagcc gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc
     8581 gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa
     8641 acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg
     8701 catccgcacg gacagatttg ggcaaatagc ttcctgccta acgaagctga gtgcgaagac
     8761 cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag
     8821 cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg
     8881 ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg
     8941 atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc
     9001 agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca
     9061 ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg
     9121 ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag
     9181 cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt
     9241 cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac
     9301 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
     9361 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
     9421 atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
     9481 cagctcgacg agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg
     9541 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg
     9601 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
     9661 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
     9721 atcgcgctta acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg
     9781 gatcagctaa tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca
     9841 ctggggacta aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac
     9901 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
     9961 gcgcgtttct tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt
    10021 gcacatgagc tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc
    10081 cgcaccgttg aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg
    10141 atggccgagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
    10201 actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc
    10261 ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag
    10321 caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
    10381 aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc
    10441 agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact
    10501 ggggtgcgac tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc
    10561 tgctcggctg tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta
    10621 ttggtcgtta tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga
    10681 cgctttcgcc aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac
    10741 gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg
    10801 atggcgatca gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg
    10861 ataaccgtat ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga
    10921 ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc
    10981 agattctggc ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga
    11041 aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc
    11101 ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca
    11161 aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga
    11221 aggtctacac caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac
    11281 cgtcgcgggg aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc
    11341 tgccggacag cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg
    11401 aagagtattc cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca
    11461 tgagggcttt atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca
    11521 atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc
    11581 tgttgcttcg tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt
    11641 aagcatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa
    11701 gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca
    11761 aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct
    11821 gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg
    11881 ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg
    11941 aacaaagcgg aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt
    12001 tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg
    12061 cgttacgcga aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt
    12121 gaccgcgtga tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc
    12181 gtaatcatcg ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg
    12241 agcgaagaag agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc
    12301 gacgagaatt tcaaaccgct gagacgctat tatctgggta atgctgacga gatcgcggcg
    12361 aaagcagcag ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg
    12421 agaaagccga ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat
    12481 tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc
    12541 tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt
    12601 gacgtaacag agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt
    12661 aggccagcgg ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca
    12721 tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat
    12781 ccatctgctt tttgaattgt ttggacgaat tagcatggct gaaatcgatc atcacctgag
    12841 ctggcagacc tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt
    12901 taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa
    12961 tcgccgaatg cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa
    13021 tggcatcgat agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag
    13081 aaagaccaga cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc
    13141 cccagctcat caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg
    13201 ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt
    13261 tgatctggaa gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg
    13321 tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac
    13381 gcagcgccag caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg
    13441 ggccaatcac caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc
    13501 gggcatgggc aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga
    13561 caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt
    13621 ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg
    13681 tcagaatgtg taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa
    13741 aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg
    13801 tttgttaatt atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca
    13861 atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata
    13921 acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag
    13981 ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc
    14041 aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa
    14101 taacgtcgac aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg
    14161 ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa
    14221 agatggtcga tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga
    14281 tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag
    14341 agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa
    14401 gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac
    14461 ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt
    14521 atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg
    14581 ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca
    14641 cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg
    14701 tttgtgaatg ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa
    14761 cgcttccgca cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg
    14821 ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc
    14881 aacgtacgag cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt
    14941 attgccgcgt tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata
    15001 ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa
    15061 ttacttgaag gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg
    15121 gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg
    15181 gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag
    15241 atccaacact acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat
    15301 caaccttgtc ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat
    15361 caccattaat gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg
    15421 ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc
    15481 tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc
    15541 acatacttac gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc
    15601 cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc
    15661 atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc
    15721 agaccaatcg aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag
    15781 cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa
    15841 atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag
    15901 aaaataatgc caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc
    15961 agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag
    16021 agatcataac cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa
    16081 tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc
    16141 cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac
    16201 atgaacctcg tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt
    16261 catggccatc cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg
    16321 tgcttccagt aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc
    16381 ggttgccaca ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt
    16441 ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat
    16501 ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg
    16561 cacaatgcag gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg
    16621 ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat
    16681 aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga
    16741 agtgttggcg tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc
    16801 ttcaacaggg cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt
    16861 tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa
    16921 agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc
    16981 accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac
    17041 cgcattacgt tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat
    17101 gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac
    17161 gctcatctta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc
    17221 gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag
    17281 tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga
    17341 tgtcatcagg tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg
    17401 gcgctttttt
//
LOCUS       DADLTY010000085        17265 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.085, whole genome shotgun sequence.
ACCESSION   DADLTY010000085 DADLTY010000000
VERSION     DADLTY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.085"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..1378)
                     /locus_tag="JN334_002389"
     CDS             complement(<1..1378)
                     /locus_tag="JN334_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAY7438503.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISE"
     gene            complement(1452..2078)
                     /locus_tag="JN334_002390"
     CDS             complement(1452..2078)
                     /locus_tag="JN334_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAY7438504.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            2219..2578
                     /locus_tag="JN334_002391"
     CDS             2219..2578
                     /locus_tag="JN334_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HAY7438505.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(2581..2850)
                     /locus_tag="JN334_002392"
     CDS             complement(2581..2850)
                     /locus_tag="JN334_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HAY7438506.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(2840..3436)
                     /gene="rsmD"
                     /locus_tag="JN334_002393"
     CDS             complement(2840..3436)
                     /gene="rsmD"
                     /locus_tag="JN334_002393"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HAY7438507.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            3586..5082
                     /gene="ftsY"
                     /locus_tag="JN334_002394"
     CDS             3586..5082
                     /gene="ftsY"
                     /locus_tag="JN334_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAY7438508.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            5085..5753
                     /gene="ftsE"
                     /locus_tag="JN334_002395"
     CDS             5085..5753
                     /gene="ftsE"
                     /locus_tag="JN334_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAY7438509.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            5746..6804
                     /gene="ftsX"
                     /locus_tag="JN334_002396"
     CDS             5746..6804
                     /gene="ftsX"
                     /locus_tag="JN334_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HAY7438510.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            7049..7903
                     /gene="rpoH"
                     /locus_tag="JN334_002397"
     CDS             7049..7903
                     /gene="rpoH"
                     /locus_tag="JN334_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAY7438511.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            8174..9277
                     /locus_tag="JN334_002398"
     CDS             8174..9277
                     /locus_tag="JN334_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY7438512.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(9476..9859)
                     /gene="panM"
                     /locus_tag="JN334_002399"
     CDS             complement(9476..9859)
                     /gene="panM"
                     /locus_tag="JN334_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HAY7438513.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            10283..11392
                     /gene="livk"
                     /locus_tag="JN334_002400"
     CDS             10283..11392
                     /gene="livk"
                     /locus_tag="JN334_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HAY7438514.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            11440..12366
                     /gene="livH"
                     /locus_tag="JN334_002401"
     CDS             11440..12366
                     /gene="livH"
                     /locus_tag="JN334_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HAY7438515.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            12363..13640
                     /locus_tag="JN334_002402"
     CDS             12363..13640
                     /locus_tag="JN334_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HAY7438516.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            13637..14404
                     /gene="livG"
                     /locus_tag="JN334_002403"
     CDS             13637..14404
                     /gene="livG"
                     /locus_tag="JN334_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HAY7438517.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            14406..15119
                     /gene="livF"
                     /locus_tag="JN334_002404"
     CDS             14406..15119
                     /gene="livF"
                     /locus_tag="JN334_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HAY7438518.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            15516..16832
                     /gene="ugpB"
                     /locus_tag="JN334_002405"
     CDS             15516..16832
                     /gene="ugpB"
                     /locus_tag="JN334_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HAY7438519.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            16930..>17265
                     /locus_tag="JN334_002406"
     CDS             16930..>17265
                     /locus_tag="JN334_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="with UgpEC is involved in the uptake of
                     glycerol-3-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter permease"
                     /protein_id="HAY7438520.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLML"
BASE COUNT         4130 a         4385 c         4800 g         3950 t
ORIGIN      
        1 attcactaat acccgttgcc ggatgaatcg cggtaacgcg cggtttaccg acggtcagcg
       61 taccggtttt atcaaacgcc acttgggtaa cacgacctag ttgttccagc gccgcaccgc
      121 ctttaattaa cgccccacga cgcgctgctg ctgccagccc ggaggttatc gccgcaggcg
      181 ttgagataac taacgcacac gggcaaccaa tcagcagcag cgtcagacct ttataaatcc
      241 actcctgcca gctggcggca aacagcagcg gcggcactag cgttaccagc agggcgacgg
      301 ccataatcgc tggcgtataa atacggctga aacggtcgat aaaccgctca atgggggcgc
      361 ggcgctcttc ggcttcttcg atcagtttca gaatccggtc aatggcgctg gcacccggtt
      421 ctgacagcac ttccagcgtt accagacggt ctacgctggt ggctcctgca ggaactttat
      481 cgcccgtcgc gcgctccacc ggaatggatt ctccggttag ggcgctttca tcaaaactgg
      541 caaacggtga gagcagttta ccgtcggcag gcaaacgccc acctgcggcg acttcaatca
      601 catcgccagg ccgcaggctg ttaatcgcca cctcttcccg ctcaccgtta cgcaggcgcg
      661 tggcggtttc tggtttcagc gccattaacg cgctaacgcc ctgacgcgcg cggctggcgg
      721 cccagccttc cagtcgttca ccaatcaaaa acagcagcaa caccatcgca gcttcagccg
      781 ttgcgccaat aaacagtgca ccaatagcgg ctacgctcat taaggtttca atggcgaagt
      841 agctgccgga tttgatcagc cgtaatgcct gacgagcaat cgggtacagc ccaactagcg
      901 tggtcgcgat aaacgccagt tgcccgaacg gatgattgaa ctgctccaga ccccagctga
      961 ttgccatcat cacgataagc gtaatcagcg gcagattctc tttcaggcgc gatgcttgtg
     1021 gctcgtcggc ggcctgctca tcgcgcaggg aatagcccgc tttttgcacc gcagattcaa
     1081 cctgggcacg gatgtcattg tcggcatcaa ccaccagttt ttcggtggcg aacaacacct
     1141 gcacctgatt cacgcctgca agctggcgca cggcattttc taccttgcgc gcacaggcgg
     1201 cgcagtccat gccgctgact ttccagctat agcgggtgcc ggagacgttt tcagagagcg
     1261 ttggcgagct ggagcatgcg ccgtcgcagc aacagtcgtt ggcgttctgt accgtggtta
     1321 gcggtttgaa cgcagcaaat tgaggggctt tcttgccgtg attgtcaggt gtcgacatgg
     1381 catcctccgg ttaagttttt tctcattaac cgaaggatac actctggagt cgactccaga
     1441 gtcaagtttt atcagagata cagtgagcgg acgatgagga agtgaccggc aaagtagcag
     1501 gccgcagcaa tcgcgttatc cgcacggaag cgtcggcgat agtggctccc cagccagaca
     1561 aagttactga taaacagcaa cgaagcgcca acaaacgcag agagcgccgg agcggtcgga
     1621 cggaagaacc acagttcacc tgccagccac accatcacca gcgtcatgcc gataaacgtg
     1681 cagataggcc aacggtactc ttccagccgc gtccagataa tcgccagtag cagcgcaccc
     1741 agaaccagca acaccagtgg cagaggccag aagaaagaga gcgtcatctg actggcgaaa
     1801 tagatggtgt acagcaggtg cgagaggaaa aacgcgccga tggcgtacat cagacgttga
     1861 cgtggcaaca gggttagcgc atcgcccagc agtgaggcgc acagtcccgc cagcaccaga
     1921 tagctaatgg cgtcgaacat cggtgcttgc caggccagta acagcaggag aagaagggtt
     1981 aacggtttaa acacccagcg ttgccaggtt ggcccacgat acgatgcatc cacagatagc
     2041 catgcggaaa gacagacagc gataaacgac caaagcatct tagttccttg aattcgttat
     2101 tttttcgtaa gcagaatgcc tacggtcttg cttccagttt agtggcgagg gtggacggat
     2161 gacaacacca taactggcag ggtatgctta attccgcatt ttccgatgag ggataaagat
     2221 gagtaaacca cctcttttct ttattgttat cattggctta attgtcgtcg ccgcatcgtt
     2281 tcgttttatg caacagcgac gggaaaaagc tgataatgat atggctccgc tccagcaaaa
     2341 gctggtggtg gtgagcaaca agcgggaaaa accgattaac gatcgccgtt cgcgccagca
     2401 ggaagtgact ccggcaggca ccagtatgcg ctatgaggca agcttcaaac cgcaaagcgg
     2461 aggaatggag cagacgtttc gcctcgacgc ccagcagtac cacgccctga cagtgggcga
     2521 taaaggtacg ctgagctata aaggaacgcg ctttgttagc tttgttggcg aacaataagg
     2581 ttatttttta actttaaatt tcttctgcca gaccagcagt tcaaacacgc cgaaaaggaa
     2641 aatccgcacc ttttcggcgg tggtcatctg cgggccatct ttcggtaaag tggatttcaa
     2701 tagcgccagc tgcataccat gcatcagcac ggtaaaaatc agcgccacgt taacgaaaat
     2761 atttagcggg cgcgggaagg gatgcaccag attgaggatt aaaaatcccc aaacgcagag
     2821 cattaacaaa cgaccaatat taatcagcat cactttctcc ttgtgcttcg tgttgataca
     2881 gccgataagc cacctgaccc gccacttttt cccgatgtaa tgaccagttt gccggaacag
     2941 tgggcagacc gttttcgact tcgctttcga cataaatcag ggcttcgtca gccagccagc
     3001 cgttatcttc cagtaaattt atcgtctctt ctaacaagcc acggcggaac ggtggatcga
     3061 caaacacgat attatgcggt gtgccttttt gcgccaggaa tgacattgcg ttgctgttca
     3121 ccacgcgtgc attgcctgct tttagtgtcg ccagattctt aattaactgc tgagaaacca
     3181 cgcgatccat ctcaatcaac gttgcccccg cagcgtagcg cgataacgct tccagcccca
     3241 gcgcgccgct cccggcgaag caatccagac attgggcgtc aacaatgacc ggagccagcc
     3301 agttaaacaa cgtttcgcgt acgcggtcgg tggtggggcg cagacctggg ctatcaggaa
     3361 ccgggagttt acggcctcgc cactgcccgc caataatgcg gatttggccg ctgccggaat
     3421 gattcggttt tttcatgata aattgctcaa tccgccagat aacacatact tgcaggcggt
     3481 gatgtgaatt aagttaagtg atagactatt tcatcatttt tttagctgct atgtacatag
     3541 cgttaacgct gtgccatgaa gcaacagcga ggagtgtagt cgcaaatggc gaaagaaaaa
     3601 agacgtggct ttttttcctg gctgggcttt ggtcaaaaag agcagacccc ggaaaaagag
     3661 acagaagttc agaatgaaca accggttgta gaagaaatcg ttcaggcgca agagcctgcg
     3721 aaggcttctg aacaagccgt tgaagagcag ccgcaggcgc atactgaagc cgaggcggaa
     3781 acttttgctg ccgacgttgt ggaagtcact gaacaggttg ttgaaagtga aaaagcgcag
     3841 cctgaagcgg aagttgttgc acagccggaa ccggtcgtag aagaaacgcc ggagccagtg
     3901 gctatcgaac gtgaagagct gccgttgccg gaagacgtca acgccgaaga ggtttcgcca
     3961 gaagagtggc aggctgaagc ggaaaccgta gagattgtcg aagcggcaga agaagaagcg
     4021 gctaaagaag aaatcaccga cgaagagctg gaagcacagg cgctggctgc cgaagcggca
     4081 gaagaggcgg tgatggtagt tcctccggca gaagaagagc agccggttgc agaaattgcc
     4141 caggagcaag aaaaaccgac caaagaaggc ttcttcgcgc gcctgaaacg cagcctgtta
     4201 aaaaccaaag aaaatctcgg ttccggattt atcagcctgt tccgcggtaa aaaaatcgac
     4261 gatgatctgt ttgaagagct ggaagaacag ctgttgatcg ccgatgtggg cgtggaaacc
     4321 acacgtaaaa ttatcaccaa tctgacggaa ggcgcatccc gcaagcagct tcgtgatgcc
     4381 gaggcgctct atggcctgct gaaagaagag atgggcgaga ttctggcgaa agtcgatgag
     4441 ccgctgaatg ttgaagataa aacgccgttc gtgatcctga tggtgggcgt caacggtgtg
     4501 ggtaaaacca cgacgattgg taagctggcg cgtcagtttg agcagcaggg taaatcggtg
     4561 atgctggcgg cgggcgatac tttccgtgca gctgcggttg aacagcttca ggtctggggt
     4621 cagcgcaaca atattccggt gattgcccag cataccggtg cggattccgc ctctgttatc
     4681 ttcgacgcca ttcaggcagc taaagcgcgt aatatcgacg tcctgattgc cgataccgcc
     4741 ggacgtctgc agaacaaatc gcatctgatg gaagagttga agaaaatcgt ccgcgtgatg
     4801 aagaaactcg acgttgaagc gccgcatgaa gttatgctga ctattgatgc cagcaccggg
     4861 cagaacgcgg taagccaggc caaactgttc cacgaagctg ttggcttaac cggcatcacg
     4921 ctaacgaaac tggacggcac ggcgaaaggc ggggtaattt tctcggtggc tgaccagttt
     4981 ggtatcccta tccgctacat tggtgtcggc gaacgtattg aggatttgcg tccgtttaag
     5041 gcggacgact ttatagaggc actttttgcc cgagaggatt aacaatgatt cgctttgaac
     5101 atgtcagcaa ggcttatctc ggtgggagac aggcgctgca gggcgttacg ttccatatgc
     5161 agccgggtga gatggcgttt ctgaccggtc attccggcgc agggaaaagt accctcctga
     5221 agctgatctg tgggattgag cggcccagcg ccgggaaaat ctggtttagc ggccatgaca
     5281 tcacgcgtct gaaaaaccgt gaagttccgt ttctgcgccg ccagattggc atgattttcc
     5341 aggatcacca tctactgatg gaccgtactg tctacgataa cgtggcgatc ccgctgatta
     5401 tcgctggtgc cagcggtgac gatattcgtc gccgggtgtc ggcggcactg gataaagtcg
     5461 ggctactgga caaagcgaag aacttcccta ttcagctttc cggcggtgaa caacagcgtg
     5521 ttggcattgc ccgcgcggtg gtgaataagc ccgcggtact gctggcggac gaaccgaccg
     5581 gtaacctgga cgacgcgttg tcggaaggga ttctacgtct gtttgaagag tttaaccgcg
     5641 ttggggtaac cgtattgatg gcaacgcacg acattaatct gatttcacgg cgttcctatc
     5701 gcatgctcac cctgagcgat ggtcacttgc atggaggcgt gggccatgaa taagcgcgat
     5761 gcaatcaatc atattcggca gtttggtggg cgtctggatc gcttccgtaa atcggtcggc
     5821 ggctcaggcg acggcggtcg taacgcaccg aaacgcgcga aatcctcgcc aaaaccggta
     5881 aatcgcaaaa ccaacgtttt caacgaacag gtgcgctatg ccttccacgg cgcattgcag
     5941 gatctgaaaa gcaaaccgtt cgccacgttt ttaacggtga tggttatcgc catctccctg
     6001 acgctgccca gcgtctgtta tatggtgtac aaaaacgtta atcaggcggc gacgcagtat
     6061 tatccgtcac cgcaaatcac tgtttacttg caaaaaacgc tggacgatga cgccgctgcg
     6121 ggcgtggtgg cacagttgca ggccgagcaa ggcgtggaga aagtgaacta tctttctcgt
     6181 gaagacgcac tgggcgagtt ccgtaactgg tctggttttg gtggtgcgct ggatatgctg
     6241 gaagaaaacc cgcttccggc agtggcggtg gtgatcccga aactcgattt tcaggggacg
     6301 gaatcactca atacgctgcg tgatcgtatc acgcagatta acggtattga cgaagtgcgg
     6361 atggatgaca gctggtttgc ccgtctggcg gcgttgaccg ggctggttgg gcgcgtttcg
     6421 gcgatgatcg gcgtgttgat ggtggcggca gtgttcctcg tcatcggcaa cagcgtacgt
     6481 ctgagcatct ttgctcgccg tgactccatc aacgtgcaga aactgattgg tgcgacggat
     6541 ggtttcatcc ttcgcccgtt cctgtacggc ggtgcgctgc tgggattttc tggcgcattg
     6601 ttgtcattaa ttttgtcaga aattctggta ctgcgtttgt cgtcagcggt ggcggaagtg
     6661 gcacaggttt tcggaacgaa gtttgatatc aatggtttat cgttcgatga atgcctgcta
     6721 ttgctgctgg tatgctcgat gattggctgg gtggcagcgt ggcttgccac ggtacaacat
     6781 ttacgccact ttacgcctga ataataaaag cgtgttatac tctttccctg caatgggttc
     6841 cgtagcaggg aaagagaccc cgttgtctct tcccggtatt tcatctctat gtcacatttt
     6901 gtgcgtaatt tattcacaag cttgcattga acttgtggat aaaatcacgg tctgataaaa
     6961 cagtgaatga taacctcgtt gctcttaagc tttggcacag ttgttgctac cactgaagcg
     7021 ccagaagata tcgattgaga ggatttgaat gactgacaaa atgcaaagtt tagctttagc
     7081 cccagttggc aacctggatt cctacatccg ggcagctaac gcgtggccga tgttgtcggc
     7141 tgacgaggag cgggcgctgg ctgaaaagct gcattaccat ggcgatctgg aagcagctaa
     7201 aacgctgatc ctgtctcacc tgcggtttgt tgttcatatt gctcgtaatt atgcgggcta
     7261 tggcctgcca caggcggatt tgattcagga aggtaacatc ggcctgatga aagcagtgcg
     7321 ccgttttaac ccggaagtgg gtgtgcgcct ggtctccttc gccgtccact ggatcaaagc
     7381 agagatccac gaatacgttc tgcgtaactg gcgtatcgtc aaagttgcga ccaccaaagc
     7441 gcagcgcaaa ctgttcttca acctgcgtaa aaccaagcag cgtctgggct ggtttaacca
     7501 ggatgaagtc gaaatggtgg cccgtgaact gggcgtaacc agcaaagacg tacgtgagat
     7561 ggaatcacgt atggcggcac aggacatgac ctttgacctg tcttccgacg acgattccga
     7621 cagccagccg atggcaccgg tgctctatct gcaggataaa tcatctaact ttgccgacgg
     7681 catcgaagat gataactggg aagagcaggc ggcaaaccgt ctgaccgacg cgatgcatgg
     7741 tctggacgaa cgcagccagg acatcatccg tgcgcgctgg ctggacgaag acaacaagtc
     7801 cacgttgcag gaactggctg accgttacgg tgtttccgct gaacgtgtgc gccaactgga
     7861 aaagaacgcg atgaaaaaac ttcgcgccgc tattgaagcg taatttccgc tattaagcag
     7921 agaaccctgg atgagagtcc ggggtttttg ttttttgggc ctctacaata atcaattccc
     7981 cctccggcaa aacgccaatc cccacgcaga ttgttaataa actgtcaaaa tagctattcc
     8041 aatatcataa aaatcgggta tgttttagca gagtatgctg ctaaagcacg ggtagtcatg
     8101 cataaaacga aataaagtgc tgaacaacaa catcacaaca cacgtaataa ccagaagaat
     8161 ggggattctc aggatgaaca taaagggtaa agcgttactg gcaggatgta tcgcgctggc
     8221 attcagcaat atggctctgg cagaagatat taaagtcgcg gtcgtgggtg caatgtccgg
     8281 tccggttgcg cagtacggtg accaggagtt taccggcgca gagcaggcgg ttgcggatat
     8341 caacgctaaa ggcggcatta aaggcaacaa actgcaaatc gtaaaatatg acgatgcctg
     8401 tgacccgaaa caggcggttg cggtggcgaa caaagtcgtt aacgacggca ttaaatatgt
     8461 gattggtcac ctctgttctt catcaacgca gcctgcgtct gacatctacg aagacgaagg
     8521 cattttaatg atcaccccag cggcaaccgc gccggagctg accgcccgtg gctatcagct
     8581 gatcctgcgc accaccggcc tggactccga ccaggggccg acggcggcga aatatattct
     8641 tgagaaagtg aaaccgcagc gtattgctat cgttcacgac aaacagcaat acggcgaagg
     8701 tctggcacga gcggtgcagg acggcctgaa gaaaggcaat gctaacgtgg tgttctttga
     8761 tggtatcacc gccggggaaa aagatttctc aacgctggtg gcgcgtctga aaaaagagaa
     8821 tatcgacttc gtttactacg gcggttatca cccggaaatg gggcaaatcc tgcgtcaggc
     8881 acgcgcggca gggctgaaaa ctcagtttat ggggccggaa ggtgtggcta acgtttcgct
     8941 gtctaacatt gcgggcgaat cagcggaagg gctgctggtg accaagccga agaactacga
     9001 tcaggttccg gcgaacaaac ccattgttga tgcgatcaaa gcgaaaaaac aggacccaag
     9061 cggcgcattc gtctggacca cctacgccgc gctgcaatct ttgcaggcgg gcctgaatca
     9121 gtctgacgat ccggctgaaa tcgccaaata cctaaaagcg aactccgtgg ataccgtgat
     9181 ggggccactg acctgggatg agaaaggcga tctaaagggc tttgagttcg gcgtatttga
     9241 ctggcacgcc aacggcacgg cgaccgatgc gaagtaattg ttaatcggca actttgggtt
     9301 gccgccaaat tgctaatatc gaatacgttg cttcatgccg gatgcggcgt aaacgcctta
     9361 tccggcctac aagaaccaaa gaaatcagta aattgcagca cacattatag gcctgataag
     9421 cgtagcgcat caggcaattt ctcgttggtc atcacttttg aaatcaactt gacgattaac
     9481 gcttctccca gccgccctgc tgtgccgtaa atcccagtgc ctgcataaac gccgtcatca
     9541 caccgcgatc ttccacgcct gcatccgcca tccaccagca tgaaacgcca ggattgttac
     9601 gcaaaacctc ttccagcaga tattgcccca caccgcgacg gcgggtgact tcccgcacgc
     9661 gtagagaatc cagtgctccc tcggtgccgc ttaaggttac ccgcacggcc gcaagcagac
     9721 gctcgttaaa acgcgcggcg tagatacggt ggttatcgtc aacctgtaaa gaggaagggg
     9781 aatactccgg ccagatcttt tgcaggtcaa tccggtcctg gtcgctaaag ttttctaatc
     9841 gaatgatggt cagcttcatg ggtaacccgt gtaaatcaca aaagtggaac cagtgtagcg
     9901 aaataattta atcggaggct ttctcttttt tatttctttt ggcaggtgat taatttttta
     9961 acagcaataa ttacaaagtt aaaacattag agaatgaaaa atgtccagca taatcccctg
    10021 aatgatagtg aattattccg ccctttgtgc cgttatttta tgctgacaaa ggcacttttt
    10081 tctgtttatc tatcaataaa ttcagaatat tatctgttct taatcgactg aaaaataggg
    10141 attttaatcg ctatcatcac aaaatactgc gctaacccct taatcagaca ggcaaaaaca
    10201 gtgcagtata aaaaaagaac agtctgattt gttaacacat aaaaacaaag caacacaaca
    10261 tcacgaatgg ggatttttga ctatgaaacg gaatgcgaaa actatcatcg cagggatgat
    10321 tgcactggca atttcacaca ccgctatggc tgacgatatt aaagtcgccg ttgtcggcgc
    10381 gatgtccggc ccgattgccc agtggggcga tatggaattt aacggcgcgc gtcaggcgat
    10441 taaagacatt aatgtcaaag ggggaattaa gggcgacaag ctggttggcg tggaatatga
    10501 cgacgcctgc gacccgaaac aagccgttgc ggtcgccaac aaaatcgtta acgacggcat
    10561 taaatacgtt attggtcatc tgtgttcttc ctctacccag ccagcgtcgg atatctacga
    10621 agacgaaggt attctgatga tctcgccggg agcgaccaac ccggagctga cccaacgcgg
    10681 ttatcaacac attatgcgta ctgccgggct ggactcttcc caggggccta cggcggcaaa
    10741 atacattctt gagacggtga agccccagcg catcgccatc attcacgaca aacaacagta
    10801 tggcgaaggg ctggcgcgtt cggtgcagga cgggctgaaa gcggctaacg ccaacgtcgt
    10861 cttcttcgac ggtattaccg ccggggagaa agatttctcc gcgctgatcg cccgcctgaa
    10921 aaaagaaaat atcgacttcg tttactacgg cggttactac ccggaaatgg ggcagatgct
    10981 gcgccaagcc cgttccgttg gcctgaaaac ccagtttatg gggccggaag gtgtcggtaa
    11041 cgcatcgctg tcgaacattg ccggtgatgc cgccgaaggc atgttggtca ctatgccaaa
    11101 acgctatgac caggatccgg caaaccaggg catcgttgat gcgctgaaag cagacaagaa
    11161 agatccgtcc gggccttatg tctggatcac ttacgcggca gtgcaatctc tggcaactgc
    11221 ccttgagcgt accggcagcg atgagccgct ggcgctggtg aaagatttaa aagctaacgg
    11281 tgcaaacacc gtgattgggc cgctgaactg ggatgaaaaa ggcgatctta agggatttga
    11341 ttttggtgtc ttccagtggc acgccgacgg ttcatccacg gcagccaagt gatcatccca
    11401 ccgcccgtaa aatgcgggcg ggtttagaaa ggttacctta tgtctgagca gtttttgtat
    11461 ttcttgcagc agatgtttaa cggcgtcacg ctgggcagta cctacgcgct gatagccatc
    11521 ggctacacca tggtttacgg cattatcggc atgatcaact tcgcccacgg cgaggtttat
    11581 atgattggca gctacgtctc atttatgatc atcgccgcgc tgatgatgat gggcattgat
    11641 accggctggc tgctggtggc ggcgggattc gtcggcgcaa tcgtcatcgc cagcgcctac
    11701 ggctggagta tcgaacgggt agcttaccgc ccggtgcgta actctaagcg cctgattgcg
    11761 cttatttccg caatcggtat gtccatcttc ctgcaaaact acgtcagcct gaccgaaggt
    11821 tcgcgcgacg tggcgctgcc gagcctgttt aacggtcagt gggtggtggg ccatagcgaa
    11881 aacttctctg cctctattac caccatgcag gcggtgatct ggattgtcac cttcctcgcc
    11941 atgctggcgc tgacgatttt cattcgctat tcccgtatgg ggcgcgcctg tcgtgcctgc
    12001 gcggaagatc tgaaaatggc gagcctgctt ggcatcaaca ccgaccgggt gattgctttg
    12061 acctttgtta ttggcgcggc gatggcggca gtggctggcg tactgctcgg tcagttctac
    12121 tgcgtcatta acccctatat cggctttatg gccgggatga aagcctttac cgctgcggtg
    12181 ctcggtggga ttggcagcat tccaggggcg atgattggcg ggctgatcct ggggattgcg
    12241 gaggcgctct cttctgccta tctgagtacg gaatataaag atgtggtctc attcgccctg
    12301 ctgattctgg tgctgctggt gatgccgacc ggtattctgg gtcgcccgga ggtagagaaa
    12361 gtatgaaacc gatgcatatt gcaatggcac tgctctctgc cgcgatgttc tttgtcctgg
    12421 cgggcgtctt tatgggcgta caactggagc tggatggcac caaactggtg gtcgacacgg
    12481 cttcagacat ccgttggcag tggatgttta tcggcacggc ggtggtcttt ttcttccagc
    12541 ttttgcgacc ggctttccag aaagggttga aaagcgtttc cgggccgaag tttattctgc
    12601 ccgcgattga tgggtccacg gtgaagcaaa agctgttcct cgtggcgctg ctggtgcttg
    12661 tggtggcgtg gccgtttatg gtgtcacgtg ggacggtgga tatcgccact ctgaccatga
    12721 tctacattat cctcggtctc gggctgaatg tggttgttgg tctttctggt ctgctggtgc
    12781 tggggtacgg cggtttttac gccatcggcg cttacacttt tgcgctgctc aatcactatt
    12841 acggtctggg gttctggacc tgtttgccga ttgccggact gatggcagcg gcggcgggtt
    12901 tcctgctcgg ttttccggtg ctgcgtctgc gcggtgacta tctggcgatc gttacccttg
    12961 gtttcggcga aattgtgcgc atattgctgc tcaataacac tgaaatcacc ggcggcccga
    13021 acggaatcag tcagatcccg aaaccgacac tcttcggact cgagttcagc cgtaccgctc
    13081 gtgaaggcgg ctgggacacg ttcagtaatt tctttggcct gaaatacgat ccgtccgatc
    13141 gcgttatctt cctctacctg gtggcgttgc tgctggtggt gttaagcctg tttgtcatta
    13201 accgcctgct gcggatgccg ctgggccgcg cgtgggaagc gttgcgtgaa gacgagatcg
    13261 cctgtcgttc gctgggctta agcccgcgcc gtatcaagct gactgctttt accatcagcg
    13321 ccgcatttgc cggttttgcc ggaacgctgt ttgcggcgcg tcagggcttt gtcagcccgg
    13381 aatccttcac ctttgctgaa tcggcctttg tgctggcgat agtggtgctc ggcggtatgg
    13441 gctcgcaatt tgcagtgatt ctggcggctg ttctgctggt ggtgtcgcgc gaattgatgc
    13501 gtgatttcaa cgaatacagc atgttaatgc tcggtggttt gatggtgctg atgatgatct
    13561 ggcgtccgca gggtttgctg cccatgacgc gcccgcaact gaagctgaaa aacggcgcag
    13621 cgaaaggaga gcaggcatga gtcagccatt attatctgtt aacggtctga tgatgcgctt
    13681 cggcggcctg ctggcggtga acaacgtcaa tcttgaactg tacccgcagg aaatcgtctc
    13741 gttaatcggc cctaacggtg ccggaaaaac cacggttttt aactgcctga ccggtttcta
    13801 caaacccacc ggcggcacca ttttactgcg cgatcagcat cttgaaggtt taccggggca
    13861 gcaaattgct cgcatgggcg tggtgcgcac cttccagcat gtgcgtctgt tccgtgaaat
    13921 gacggtaatt gaaaacctgc tggtggcgca gcatcagcaa ctgaaaaccg ggctgttctc
    13981 tggcctgttg aaaacgcctt ctttccgtcg cgcccagagc gaagcgctcg accgcgccgc
    14041 cacctggctt gagcgcattg gtttgctgga acacgccaac cgtcaggcga gtaacctggc
    14101 ctatggtgac caacgccgtc tggagattgc ccgctgcatg gtgacgcagc cggagatttt
    14161 aatgctcgac gaaccggcgg caggtcttaa cccaaaagaa accaaagagc tggatgagct
    14221 gattgccgaa ctgcgcaatc atcacaacac cactattttg ttgattgaac acgatatgaa
    14281 gctggtgatg gggatttcag accgaattta tgtggtcaat caggggacgc cactggcaaa
    14341 cggtacgccg gagcaaatcc gtaataaccc ggacgtgatc cgtgcctatt taggtgaggc
    14401 ataagatgga aaaagtcatg ttgtcctttg acaaagtcag cgcccactac ggcaaaatcc
    14461 aggcgctgca tgaggtgagc ctgcatatca atcagggcga gattgtcact ctcattggcg
    14521 cgaacggggc ggggaaaacc accttgctcg gcacgttatg cggcgatccg cgcgccacca
    14581 gcgggcgaat tgtgtttgat gataaagaca ttaccgactg gcagacggcg aaaatcatgc
    14641 gcgaagcggt agcgattgtc ccggaagggc gtcgcgtctt ctcgcggatg acggtggaag
    14701 agaacctggc gatgggcggc ttctttgccg aacgtgacca gtttcaggag cgcataaagt
    14761 gggtgtatga actgtttcca cgtctgcatg agcgccgtgt tcagcgggcg ggcaccatgt
    14821 ccggcggtga acagcagatg ctggcgattg gtcgcgcgct gatgagcaat ccgcgtttgt
    14881 tgttacttga tgagccatcg ctcggtcttg cgccgattat catccagcaa attttcgaca
    14941 ccatcgagca gctgcgcgag caggggatga ctatcttcct cgtcgagcag aacgccaacc
    15001 aggcgctaaa gctggcggat cgcggctacg tgctggaaaa cggccatgtg gtgctctccg
    15061 ataccggcga cgcgctgctg gcgaacgaag cagtgagaag tgcgtattta ggcgggtaat
    15121 aacacgttga ttgataagga gtcaaaagac tcctttgaga caggtgacaa atgcaaaatt
    15181 gcctgatgcg ctgcgcttat caggcctact gggtgagttg caatatattg aatttgcacg
    15241 atcttgtagg ccggataagg cgtttacgcc gcatccggca tgaaacgatg agtaatctgt
    15301 agagtttgat tcagaccttc tatttttccg cttatccgtg ccccatctcc cattttccct
    15361 cacccacgcc gtcaccgcct tgtcatcttt ctgacacctt actatcttac aaatgtaaca
    15421 aaaaagttat ttttctgtaa ttcgagcatg tcatgttacc ccgcgagcat aaaacgcgtg
    15481 aattcgcgca ttcggtacaa caagagagat aaacgatgaa accgttacat tatacagctt
    15541 cagcactggc gctcggactg gcgttaatgg ggaatgcaca ggcagtgacg accattccgt
    15601 tctggcattc tatggaaggg gaactgggta aagaggtgga ttctctggcc caacgtttta
    15661 acgccgaaaa tccggattac aaaattgtac cgacctataa aggcaactac gaacagaatt
    15721 taagcgcggg gattgccgta tttcgtaccg gcaacgctcc ggctattttg caggtttatg
    15781 aagttggcac cgccaccatg atggcgtcga aagccattaa accggtgtat gacgtgttta
    15841 aagaggcagg gattcagttc gatgagtcgc agtttgtgcc gacggtttcc ggttactact
    15901 ccgacagcaa aaccggccac ttactctccc agccgttcaa cagctcgact tccgttctct
    15961 attacaacaa agacgccttc aagaaagcgg ggttagaccc ggaacagccg ccgaaaacct
    16021 ggcaggatct ggcggactat tccgcaaaac tgaaagcctc cggtattaag tgcggctacg
    16081 ccagcggctg gcagggctgg atccaactgg aaaactttag cgcctggaac ggtctgccgt
    16141 ttgccagcaa aaacaacggc tttgacggca cggacgcggt gctggagttc aacaagccgg
    16201 agcaggtgaa acacatcgcc atgctcgagg agatgaacaa gaagggcgac ttcagctacg
    16261 tcggtcgtaa ggatgaatcc accgagaagt tctataacgg tgattgcgcg atgaccaccg
    16321 cctcttccgg ttctcttgcc aacattcgcg agtacgccaa atttaactac ggcgtaggta
    16381 tgatgcctta cgacgccgat gcgaaagatg cgccacaaaa cgccattatc ggcggagcca
    16441 gcctgtgggt gatgcagggc aaagataaag aaacgtatac cggcgtggcg aagtttctcg
    16501 acttcctcgc gaagccagaa aacgctgctg agtggcatca gaaaaccggt tatctgccaa
    16561 ttaccaaagc ggcgtatgac ctgacccgtg agcagggctt ttacgagaaa aacccaggtg
    16621 cggataccgc gacgcgtcag atgctgaaca agccgccgtt gccgttcacc aaagggcttc
    16681 gtctgggcaa catgccgcag atccgcgtga ttgtggatga agagcttgag agcgtgtgga
    16741 ccggtaagaa gacgccacag caggcgctgg ataccgcagt tgagcgtggg aaccagttac
    16801 tgcgccgctt tgagaaatcg acgaagtctt aatcagtgta atgccggatg cgtttcgctt
    16861 atctgacctg gcatcgcgtg taggccggat aagcgaagcg catccggcac agttcaggaa
    16921 ttaaccgtaa tgtcatcatc ccgtccggtg ttccgctcgc gctggctgcc ttatctgctg
    16981 gtcgcgccgc agctcatcat caccgttatc ttttttatct ggcctgcggg cgaagcgttg
    17041 tggtactcgc tacaaagcgt cgatccgttt ggtttctcca gccagtttgt cggtctggat
    17101 aacttcgtca cgctgtttca tgacagctat tatctcgact ccttctggac gacgataaaa
    17161 ttcagcacct ttgtcaccgt cagcggtttg ctggtatcgc tgttctttgc ggcgctggtg
    17221 gagtacatcg tgcgcggcag tcgtttctac caaaccttaa tgttg
//
LOCUS       DADLTY010000086        17265 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.086, whole genome shotgun sequence.
ACCESSION   DADLTY010000086 DADLTY010000000
VERSION     DADLTY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.086"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            445..1011
                     /gene="mntP"
                     /locus_tag="JN334_002407"
     CDS             445..1011
                     /gene="mntP"
                     /locus_tag="JN334_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HAY7438521.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="JN334_002408"
     CDS             complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="JN334_002408"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HAY7438522.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="JN334_002409"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="JN334_002409"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY7438523.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2205..2348)
                     /locus_tag="JN334_002410"
     CDS             complement(2205..2348)
                     /locus_tag="JN334_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HAY7438524.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(3019..3306)
                     /locus_tag="JN334_002411"
     CDS             complement(3019..3306)
                     /locus_tag="JN334_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438525.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(3381..3524)
                     /gene="mgrB"
                     /locus_tag="JN334_002412"
     CDS             complement(3381..3524)
                     /gene="mgrB"
                     /locus_tag="JN334_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HAY7438526.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3683..3922
                     /locus_tag="JN334_002413"
     CDS             3683..3922
                     /locus_tag="JN334_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438527.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(4065..4856)
                     /gene="kdgR"
                     /locus_tag="JN334_002414"
     CDS             complement(4065..4856)
                     /gene="kdgR"
                     /locus_tag="JN334_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HAY7438528.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            5033..6406
                     /locus_tag="JN334_002415"
     CDS             5033..6406
                     /locus_tag="JN334_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7438529.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(6452..7333)
                     /gene="htpX"
                     /locus_tag="JN334_002416"
     CDS             complement(6452..7333)
                     /gene="htpX"
                     /locus_tag="JN334_002416"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HAY7438530.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7525..9573)
                     /gene="prc"
                     /locus_tag="JN334_002417"
     CDS             complement(7525..9573)
                     /gene="prc"
                     /locus_tag="JN334_002417"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HAY7438531.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9593..10291)
                     /gene="proQ"
                     /locus_tag="JN334_002418"
     CDS             complement(9593..10291)
                     /gene="proQ"
                     /locus_tag="JN334_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HAY7438532.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10388..10885)
                     /locus_tag="JN334_002419"
     CDS             complement(10388..10885)
                     /locus_tag="JN334_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAY7438533.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            11015..12298
                     /gene="yebS"
                     /locus_tag="JN334_002420"
     CDS             11015..12298
                     /gene="yebS"
                     /locus_tag="JN334_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HAY7438534.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12267..14887
                     /gene="yebT"
                     /locus_tag="JN334_002421"
                     /pseudo
     CDS             12267..14887
                     /gene="yebT"
                     /locus_tag="JN334_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            14967..16406
                     /gene="rsmF"
                     /locus_tag="JN334_002422"
                     /pseudo
     CDS             14967..16406
                     /gene="rsmF"
                     /locus_tag="JN334_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            16524..16760
                     /locus_tag="JN334_002423"
     CDS             16524..16760
                     /locus_tag="JN334_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HAY7438535.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16781..17056
                     /locus_tag="JN334_002424"
     CDS             16781..17056
                     /locus_tag="JN334_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HAY7438536.1"
                     /translation="MAGYLSWLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDVYNTEQQCLYSMSDQRIRHGSCFPIEDFIDGFWQPAQEYGDF"
     gene            complement(17081..>17265)
                     /locus_tag="JN334_002425"
     CDS             complement(17081..>17265)
                     /locus_tag="JN334_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAY7438537.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
BASE COUNT         4187 a         4354 c         4161 g         4563 t
ORIGIN      
        1 gtatcaactc aataacttat gaatttagcc aaagctatgt ttagtgtatt tttaataatc
       61 agacatagct taggctatat tatctcttcc cttatttgtt atttatttta acgtttcatt
      121 gatatataaa cataaatgaa aatcgttatc aataaagcaa tgaaataata tattccaaca
      181 gttgttttat attctcaaaa tatgttaagg ttgcgccctc atttggggag tagccgattt
      241 ccagattccg gaaatgtacg tgtcaacata ctcgttgcaa aacgtggcac gtacggactg
      301 aagactttca gtcaggcgag accatatgca catcaatcgc tatgcctgca tgaacgcgaa
      361 aacccatcgc ggcttgttgt tcatgtatgc atgtttgctg ggggcgatga tgtgttttat
      421 ggataccccg gtcaggacgt tgtcatgaat atcactgcta ctgttcttct tgcgtttggt
      481 atgtcgatgg atgcatttgc tgcatcaatc ggtaaaggtg cccccctcca taaaccgaaa
      541 ttttctgaag cattgcgaac cggccttatt tttggtgccg tcgaaaccct gacgccgctg
      601 atcggctggg gaatgggcat gttagccagc cggtttgtcc ttgaatggaa tcactggatt
      661 gcgtttgtgc tgctgatatt cctcggcggg cgaatgatta ttgagggttt tcgtggcgca
      721 gatgatgaag atgaagagcc gcgccgtcgg cacggtttct ggctactggt aaccaccgcg
      781 attgccacca gcctggatgc catggctgtg ggtgttggtc ttgctttcct tcaggtcaac
      841 attatcgcga ccgcattggc cattggttgt gcaaccttga ttatgtcgac attagggatg
      901 atggttggtc gctttatcgg ctcaattatt gggaaaaaag cggaaattct cggcgggctg
      961 gtgctgatcg gcatcggcgt ccagatcctc tggacgcact tccacggtta ataagaacgc
     1021 tgccagaggt gaatattaaa gtccgtctgg cagtcgaaca cttcttttgc tgccagtgtt
     1081 tgccagactt ctggcttcgc acgccaggca aacggcgtca tctgcaataa tgcgacggct
     1141 tcatcaccgc gaagacgcat cggataacac aactccgcac tctgctgtaa tgtaaaacct
     1201 tccagttgtt ctgcatgagg tgcatgaaga tgtacttcat tgtaaatcag ccccttcagc
     1261 tccatcaaat gtcgcggtcc cggcgtggca gtaatgaccc agccgccggg cttcactact
     1321 cgtgctaatt cttctgcttt acacggcgcg taaatacgta ttatggcgtc catactggta
     1381 tcggaaaacg gcaaacggtg gctggaagcg acacaaagag tgacctgcgg atagcgtttc
     1441 gccgccgctt ttatcgctac cttcgagaca tccaggccaa acgtggtgat ttcgggcaac
     1501 gcatcggcaa atgcgtgtgt gtaataccct tcaccacagc caatatccag caccgccgtg
     1561 gccttttcat caagccgttc ccgcagttgg gcgacaattg catcacgcag cggctgataa
     1621 tgtccggcat ctaagaatgc gcggcgtgct tgcatcattt ccgcgctgtc gcccggatca
     1681 cgagaccgtt tatgctgaac gggcagcaga ttgacatacc cttctttcgc catatcaaac
     1741 tgatgtcgtt ggggacagat atagctgttt ttttcacgcg aaagaggctg atggcaaagt
     1801 ggacaggaaa acgacatgac aactccggca ggatactaaa ggccgcaagt gtaacgcgaa
     1861 tcacgcggcg ggcaaatatc cgaacgccag gagcgaagat aaagcatagt aaaaagcctc
     1921 gcattcgacg aggctttata gcgagattga agcgtattca cacttcagat cagtggattc
     1981 gatcagatag ctgttacgtt aacagctgcc ggacctttct ggccgtcctg aatttcgaac
     2041 tcaacgttct gaccttcagc cagagttttg aagccattac cctggatagc ggagaagtgt
     2101 acgaacacat ctttgctgcc atcagccgga gtaatgaagc caaaaccttt agactcgttg
     2161 aaccacttaa cctgaccttt aatctttgcc atttgaaaaa ttccttagat tgttttcttc
     2221 gcccgcaggc ataacataga taaaactgac acattactgc atgaggcacc aatataaggc
     2281 tcggcagaga agcggtattc aacgtcaacg tgtttactca ggacttcttt actgaaaatg
     2341 ccacacataa acagaactgt acctcgttta acccgaaatc ttttatcaca tacaacgtta
     2401 attatggcaa gccattttta aacatatctc gatcagacgc acaaatctcg atacgtcttc
     2461 cacttttttt gcacacttat gcaacggaat acgcgccgat atatcattgt gcttaacctt
     2521 gccagttcag gcagatactt aaactggcgt agtttctaac atagttcaat aagttcagtt
     2581 gttccaggcc agagggagaa gttattacat agtgcgtgca atatcacatt ttctgctatg
     2641 caatgaataa aaagttatat cactttttct cataaaacag tcagttaacg gctatcaatt
     2701 accctaaaga gaagtcaatc cccaaaggga ttgtaaattt aaaataagaa aaattgatga
     2761 gtgagcaaaa aaaatcaaga gagaaacgtt tcgctaataa ataataccac gcttaatcac
     2821 aaatctgatg taaacatcct aataaattaa tgaggaaaat atctttttct gatatcccgt
     2881 tacgcaaata aataatatga taattatttg aagtcagacc agagttttaa tttgaagcag
     2941 ttgcaccatg acagtgcgta taatcaggat gaattgagta acccttcaac gacggggtta
     3001 ctcaagattg aggaaggatt atcgttcggc gaccaggcga ataatatctt cgtcatcaac
     3061 cgatttttga ctctcaatgg tctttttatc tgctttctct ggttcgtttg cttcgcacaa
     3121 acgacgtaac agtgcatttt gacgtttttg ctgatccagc aacgcctcaa gcagttcaat
     3181 ctgttcgtta gtccgtgaac tggcacgatt gataaaaaac cacaagatga gcccgataag
     3241 aagaaccacc accgatacaa ccaaagacgc aatgttcatc acgcctgaat ttacaacttc
     3301 gttcatttca ccacctctga gtagaggcgc tattctacca ctgctggaga ggaagaaaat
     3361 ctagtgctga aaaaatgata tcaccacggg ataaactggt taatggcaca aattccgctg
     3421 aaaaattgta catcctgatc gcacatcatg ttgaatacct gcgcccaaag cagcaagcaa
     3481 gccaacacca cgacaaccag aacgacccat cgaaactttt tcactccaat ctccgcttca
     3541 cctaccttat gtcattaaac tagcatgcgt tagttaaaca gcgcctaact atgtcgatat
     3601 tcgtgctttt tcggaatgag tagcttgctt cagcgcatat tgccgctacg attaagcgaa
     3661 cataaaaaga gaagagattg taatgcgatt catcattcgc acagttatgc tgatagcact
     3721 ggtatggata ggattattac tcagcggata tggcgttttg attggaagca aagagaatgc
     3781 cgcaggatta ggtttgcaat gtacctatct gactgccaga ggcaccagca cggtacaata
     3841 cttgcacact aaaagtggtt tcttggagat aacagattgc ccactgttac gtaaaagcaa
     3901 tatcgtggtc gataatggtt aagggtaata aataaccaga ttctcactca tgagtaaaat
     3961 attcttatag caagcgcgtt taatataacg caccttcaac tttatttttc tcatgctaaa
     4021 taaagaacct gtctgtttga tattccgaca ggttcttact ctcatcagaa cggatagtcg
     4081 tgataaccca tttgggcaga aattttgcgc gctgcggtat gcaacattgc gacatattcc
     4141 tgtaaacgct cttcagagaa acgcaacgtc gggaaggaga tgctcaaacc ggcaatgacc
     4201 acgccaaagc gatcaaatac cggtaccgca atgcattgca gcccttcttc ctgctcttcg
     4261 ttatcttctc catacccctg ctcgcgcacc tggtccagaa cgggtaataa cgcttctgta
     4321 ctggtgatgg tccgctcggt actgcgttta tactccacgc cctcaagaat ttgcttcact
     4381 tcatcgcgat cgcgccatgc cagcagtacc ttaccaatcg cggtgctgta cagcggatta
     4441 cgacgcccaa tccgtgaata catgcgcaaa ttgtacatag agtcaatttt gtgaatgtaa
     4501 acaatactgt cttcgtccag tgcgccgagg tggatagttt ctttggtcag gcgggagatc
     4561 tcacgcatct ggatatctgc gctacgaatt aaatcgacgt tttgtaacgc gcgagcgccc
     4621 agttcaaaca atttcagggt cagggaatat ttctccgact ccccttcctg cgccacataa
     4681 cctaaggttt tcatggtctg taaaaagcga taaacggtgc tttttgacat catgacgcgc
     4741 tgcgacagct cggttatccc tatttcgcgc tcttcaccca gcgcctgcaa aatgccaaaa
     4801 acttttagca cggaagatac agaatcaggc tgtttatcca gatctgcgtt agccatttat
     4861 cacctcattg cgagtgtttt ataaaaatca gaactgtttt ttattataat ttcgcaccag
     4921 ggtggtcgca atccatcttt tgccggttag ttacaattct gcgacatcca ccgtgaatat
     4981 cagtgctaga atcatacccc tgttgattat tcaccaaaga tataaaattc ctatgccaaa
     5041 agttcaggcc gacggcctgc cattgcccca gcgatacggt gcgatattaa ccattgtgat
     5101 tggtatttcg atggctgtcc ttgacggcgc aatcgccaac gtcgccctgc caacaattgc
     5161 cacggacctt catgccacac cagccagttc catctgggta gtgaacgcct atcaaatcgc
     5221 cattgtcatc tccctgctct cgttttcgtt tctgggcgat atgtttggct atcgacgtat
     5281 ttataaatgc ggtctggtcg tttttctgtt gtcttcactg ttctgcgccc tttctgattc
     5341 gctgcaaatg ctcacccttg cgcgtgtcat acaaggtttc ggcggtgcag cgttgatgag
     5401 cgttaatacc gcacttatcc gcctgatcta tccacaacgt tttctgggga gagggatggg
     5461 cataaactcg tttattgttg ccgtctcttc tgctgccggg ccgacaattg ctgcagcaat
     5521 cctctccatc gcatcctgga aatggttatt tttaatcaac gtaccgttag gtattatcgc
     5581 cctgcttctg gcgatgcgtt ttctgccacc caatggttct cgcgccagta aaccccgttt
     5641 cgacctgccc agcgccgtga tgaacgcgtt aaccttcggc ctgcttatca ctgcgttgag
     5701 tggtttcgct caggggcaat cgctgacgtt aattgctgcg gaactggtgg taatggttgt
     5761 tgttggtatt ttctttattc gccgccagct ttctcttccc gtaccgctgc taccggtgga
     5821 tttactgcgt atcccgctgt tttcactttc tatttgcaca tctgtttgct ctttctgcgc
     5881 acaaatgctg gcaatggttt ccctgccctt ttacctgcaa accgtgctcg ggcgtagtga
     5941 agtcgaaaca ggtttacttc tgacaccgtg gccgttagca acgatggtga tggctcctct
     6001 ggcaggctat ttgattgaac gcgtacatgc aggattgctg ggtgctttag ggttgttcat
     6061 catggctacg gggctttttt ccctggttct gctgcccgcg tcacctgcgg atatcaatat
     6121 tatctggccg atgatcttat gtggcgctgg atttggctta ttccagtcac ccaataacca
     6181 caccattatt acctccgctc cgcgcgaacg tagcggtggg gccagtggca tgttaggaac
     6241 ggctcgtcta ctgggtcaga gtagtggcgc ggcgctggtg gcgctgatgc taaatcagtt
     6301 tggtgataat ggtacgcacg tctcgctgat ggctgcggct attctggcag tgattgctgc
     6361 ctgtgtcagt ggtttacgta tcactcagcc acgagccagg gcataaaaaa agcgcgtcga
     6421 tcaggacgcg ctttttagta tttacttcat attacttcag gtattcaccc gtacgcagag
     6481 cttcaatacg tttatccagc ggcgggtgag tcatgaacaa ctcactgagc gatttcgact
     6541 taccgttaat gcagaaagcc atcatgctgg ttgcttcttg cggttcatag ctggttttca
     6601 ggcgctgcag cgcggcaatc attttctcgc gaccaaccag ttttgccgaa ccggcatctg
     6661 catggaattc acgatgacgc gagaaccaca tggtgataat gctcgccaga ataccaaaca
     6721 ccagttccag aaccgttgca accgcaaagt agatcagcgg gttgccgttg ctctcttcac
     6781 cttcatcacg atttccgccc ataaaacccg cggcaagctg cgccagaata cgggaaataa
     6841 agataacgaa ggtgttcacc acgccctgaa tcagcgtcat ggtgaccata tcaccattgg
     6901 cgatgtggct gatttcgtga gcaattaccg cctcggcttc atccgggctc atgttctgca
     6961 gcaaaccggt gctgacagca accagagagg catcacggcg cgcaccggtt gcaaaagcgt
     7021 tgatgtccgg cgcatggtag atagccactt gcggcatagc gatccccgcc tgacgagcct
     7081 gggttgctac agtattgacc agccaacgtt ccctttcgtt acgcggttgc tcgatcactt
     7141 ccccgccaac agatcgtaat gccatccatt tggacatcag aagcgaaacg aaggaaccac
     7201 caaaaccgaa cagcaaggcc atgatcatca gcccctgaac gctgctcgac tgtatccctg
     7261 tcaggctcag taccagcccg aaaacgacca ttacggccag gttcgttagc aggaagagcg
     7321 cgattcgcat cataattttc tttttacctc agtttaacaa aacgcaatat gcgataccca
     7381 catcgtatgg gttacgcgac tattttcaag tctggatagt gcgtaagtca ccagaaagac
     7441 acaactttac attttgtagc atctgattta cggcatcttg ccgctgttaa aaaatcaggc
     7501 acaatttctt gtgcctgatt gatattactt gacgggagcg ggttgttccg cgggtctggc
     7561 tttttcaagc ttcgccagat cgagtgcgat attcaccgtc tcatccagat aaggatccgg
     7621 ctcctggtaa tctttcgata gatcatccag cttcttcaac tccggtttac cttctcgttt
     7681 aaagcgttcg ttcaaacgcg ccagacgcgt cgcatcatct tcattattct ctttctcacg
     7741 cacagcgtag ttcagagaaa cgatattgcg cttgtccttc atagcgttga agcgcgcgat
     7801 atccttcatg atgttctgga actcaggatc tttcgcgata cgcgcattat gttccttcag
     7861 cagctccggt tcaaaggcag ttaaatctcc tgatttcaca taagtcgcgg catcgatgct
     7921 atcccacggc agcgcgttat cttcgaattt ctcacccgtt tccgtttctt cattacccgt
     7981 cggcatgatg atgtctggtg ttacgccttt acgttgcgta ctgccgccgt taacgcgata
     8041 gaatttctgg atcgtgtact gcacagaacc cagcgctggc cattcaggac gtaacatctg
     8101 atcgtaaata cggttcaatg aacggtattg ctgaacggtg cctttaccaa acgtcggttc
     8161 acccacaacc agcgcacgac cgtaatcctg cattgccgcg gcaaagattt ctgaagccga
     8221 agcactgaaa cggtcaacca gtaccaccag cgggccttta tagaacacct gcccgtcggt
     8281 atcgctgtct tcacgaacct tgccgttgtt atcgcggacc tgaacaatgg gacccgcagg
     8341 aataaacaga cctgagagcg ataccgcttc ggtcaacgcc ccaccgccat tgctacgcag
     8401 gtcgatgatc acactgctga cattctgttt ttccagtttc tgcagttgaa ctttgacatc
     8461 gtctgtcaaa cccacataga agcccggaat atccagcacg ccgactttct ctttaccgac
     8521 ggtcttcacc gacattttaa ccgcgcggtc ttcgagacga atacgttcac gggtcaacgt
     8581 tacagtacgg gtcttggtcc ctttaccagc aggtaaaatt tccagacgaa ctttactgcc
     8641 cttcggccct ttaattaagg caaccacatc atcaagacgc cagccaatca cgtcaaccat
     8701 cggcttgcct gtttgaccaa caccgacaat tttgtcacca acgctgatag ctttactctt
     8761 cgctgccgga ccacctgcca ccatcgaatt gataacggtg tagtcatcat ccatttgcag
     8821 cactgcgcca ataccttcca gtgacaaact catttcagtg ttgaactgtt cggtattacg
     8881 cggggaaaga tagttggtat gcggatcgat ttcacgcgca aacgccgtca ttgccagcga
     8941 gaaaacatct tcgctgttgg tttgcgccag acgacgaatg gcaaatttgt agcggcgtgt
     9001 cagggtttca cgaatttctt tatccgtttt tcctgtcagc ttcaggctta actcgtcgaa
     9061 tttgacttta ctgtcccact gcgcgttcaa ctcagcctcg tttttcggcc agggcgcttt
     9121 gctgcggtca aggttataag tgtcgttgcc ggtgaaatcc atcggctttt ccagtaccga
     9181 caaagcgtac tggtaacgtt caaaacggcg cttttgcgcc agattgtaga gatcgtagaa
     9241 aacgtcgagt ttgcctgaac gcagttcatc gcctaactcg gttttctttt tcgcgaactg
     9301 ttcaacatcg cttgccagca gcacattgtg gctgtaatcg agcagattca ggtagcggtc
     9361 aaagattttg gccgaaaatg cctgatcgag gtcgaactgg cgataatgag aacgggtgaa
     9421 gcgcgacgtt acgcgctcac ttaccgtcgc atgctgcgtc tcttccttta ataccggaat
     9481 ttgatcagca cgcgtgatat cttctacagc gaaggtctgg cctgctattg caagcaggcc
     9541 agctaacgcg gtaagcctaa aaaacatgtt catgcctggc ccggcctccg tttcagaaca
     9601 ccaggtgttc tgcgcgcaca atcaaagaca tacccgaatt cagctggacg cggacgccgt
     9661 ctttggtgat ttctaatacg gtggcatcca tcgcgttttg acccgctttc accttcaggg
     9721 cttgtccgac agtcagagct gaaatgtcag aaaccggggt gtgctgttct tcgcgaggtg
     9781 cttttactgt ttttggcgct ttctctaccg gcttttgcgc acgaggttta cgttcagcgc
     9841 cttctttgcg gcgtggcgtg gtcggacgtg gcttgcgttc gcggcgcggt gcgtcttctt
     9901 tctcaccagc agctgcggca gcttcgcgtt ttttcgcttg ctgttcagca cgctgtgcct
     9961 gaacacgcgc tttcgcttct tcaagctgct tgcgagcatg ctctacatgt tgctcgtcca
    10021 gctcaccgca tgggttgccg tcaagatcga cacgcgttgc gccgggttta acaccgtaaa
    10081 gataacgcca gctcgaagtg tagagacgta aagcggatcg taattgcgtt ttgctcaggt
    10141 tcatttcccc ggcaacacga tcgaccaaat cctgaaaaat accaattttc agcggacgcg
    10201 cttcaccttc cgcactgaaa cagtggggaa aacgttcggc cagaaacgcg attacttctt
    10261 tactgctatt caacttaggt tgattttcca tgaaatttcc tgattacaac ggacgtagcc
    10321 aacaagcgca ggcatgaaca ggcgtcatta taatgacgct accagtaaat gctacgttat
    10381 ccgttgatta tcctgcgacg ctcgcaaaga attttttgta atccgtcgtt gcaagcactt
    10441 tttcaagctg tgccacaagc tgacgtaagc cttgctcgtc ctcgtctgta aagcgagcga
    10501 agacagtact atcgatgtcg agaacaccaa taatctgatt tttcaccacc agcggcagaa
    10561 caatttcaga attactcgcc gcatcacagg caatatgccc gtcaaacgcg tgaacatcct
    10621 cgatacgctg cacttgattg cgggcaaccg ctgtgccgca caccccgcgc ccgacgggta
    10681 tccggacaca ggcaattttg ccctgaaatg gtccgagtac cagtgtatcg tcctcaagca
    10741 aataaaaacc tgcccagtta acgtcagtga gacgctcgta taacaacgca ctggtgttcg
    10801 caagcgttgc cagaaaactg gtttctcccg ccatcagcgc gttaaagtcg cgatttaaat
    10861 ccgcgtaaaa ttctgttttg ttcattactt aatcacttgg ttgtcttacc tggaactgcc
    10921 agcctattaa aataagcatt aaatgcgtta atgctcaaga tcattcccat catgggttaa
    10981 gattaatgtt aattcttatt acatttggca cgtcatggct cttaacacac cacaaattac
    11041 gccgacaaaa aagataacag tgagggcaat cggcgaggaa ctgccgcgtg gtgattacca
    11101 acgttgcccg caatgtgaca tgctgtttag cctgcccgag ataaattctc atcaaagtgc
    11161 ctattgtccg cgctgtcagg caaaaattcg cgacgggcgc gactggtcgc taacgcgcct
    11221 ggcggcaatg gccttcacca tgctgttgct tatgccgttt gcctggggcg aaccgctgtt
    11281 gcatatctgg ctgttaggca ttcgtattga cgccaacgtc atgcaaggca tctggcaaat
    11341 gaccaaacag ggcgatgcga taacgggatc aatggtcttt ttctgcgtta ttggtgcccc
    11401 cctcattctg gtgtcctcca tagcatattt atggtttggt aaccgactgg gaatgaattt
    11461 acgtccggta ctgctgatgc ttgagcgact taaagagtgg gtaatgctgg atatctacct
    11521 ggtcggcatt ggcgttgctt ctataaaggt acaggattat gcccatatcc aggcgggtgt
    11581 cggcttgttc tcttttgtgg cgttggtgat tttaacgacg gtgacgttgt cacatcttaa
    11641 tgttgaggag ctgtgggagc gatattatcc gcagcgcccc gctacgcgta gggacgagaa
    11701 acttcgtgtc tgtcttgggt gccattttac cggctatcct gatcagcgtg gtctctgccc
    11761 gcgttgccat atcccgctac gcctgcgtcg tcgtcatagt ttgcaaaaat gctgggcggc
    11821 gctgttagcg tcaatcgttt tgttgttacc agccaaccta ttgcctattt ctatcattta
    11881 tctgaatggt ggccggcagg aagatacgat tctttccgga attatgtcgc tggcaagtag
    11941 caacattgcg gttgcgggaa tcgtgtttat cgccagtatt ctggtaccgt ttactaaagt
    12001 gatcgtcatg ttcactttac tgttgagcat tcattttaaa tgccagcaag gtttacgcac
    12061 acgcattctg ttactgcgga tggtgacctg gattggacgc tggtcgatac tcgacctgtt
    12121 tgtcatatct ttaaccatgt cgctgattaa tcgcgatcag atcctcgctt ttactatggg
    12181 accggctgcg ttttattttg gcgctgccgt aattttgact attcttgctg tggaatggct
    12241 ggacagccgc ttactttggg atgcacatga gtcaggaaac gcccgcttcg acgactgaag
    12301 cgcagattaa aaataaacgc cgtatctcac ctttctggct gctgcctttc atcgcgctaa
    12361 tgattgccgg ttggctgatt tgggacagtt atcaggaccg gggtaatacc gtcaccatcg
    12421 actttatgtc ggcggatggt attgttccgg gccgtacgcc tgttcgttat cagggcgttg
    12481 aagtcggaac agtgcaggat atcagcctca gcgacgatct tcgtaagatt gaagtcaagg
    12541 tcagcatcaa gtccgatatg aaagatgcgc tgcgcgaaga gactcagttc tggctgatga
    12601 cgccaaaagc atcgttggca ggtgtctccg ggctggacgc cctcgtcggt gggaactata
    12661 tcggcatgat gccgggtaaa ggtaaagagc aggatcactt tgtcgcgctc gatacccaac
    12721 cgaaatatcg gctggacaat ggcgatctga tgatccacct gcaagccccc gctctcggtt
    12781 cgctgaacag cggttcattg gtctatttcc gcaagatccc ggtgggaaaa gtctacgact
    12841 atgccatcaa tcccaacaag caaggcgtgg tgattgatgt cctgatcgag cggcgtttta
    12901 ccgatctggt gaaaaaaggt agccgtttct gaaacgtttc cggcgttgat gccaacgtca
    12961 gtatcagtgg cgcgaaggtg aaactggaaa gtctggcggc actggttaac ggtgcgattg
    13021 ccttcgattc acctgaagag tcgaaacctg ccgaggcgga agataccttt ggtctgtatg
    13081 aagatctggc ccacagccag cgtggagtaa taataaaact ggaactgccg agtggagcag
    13141 gattaaccgc cgactcgacg ccgttaatgt atcaggggct ggaagtcgga cagctgacta
    13201 aactggattt aaatcctggt ggtaaagtca ctggggaaat gaccgttgat ccctgcttcg
    13261 tgaaaatacc cgcatcgaat tacgcaaccc gaaattatcc cttagcgatg ctaatctcag
    13321 cgccctgctg accggaaaaa cctttgagtt ggtgcccggc gatggcgagc cacgcaaaga
    13381 gttcgttgtt gtgccgggcg aaaaagccct gctgcaagag ccggacgttc tgacactgac
    13441 cctgacagca ccggaaagtt acggtattga tgcgggtcag ccgctcattc ttcacggcgt
    13501 gcaggtaggc caggttatcg atcgtaaact caccagcaaa ggcgtcacct ttaccgtcgc
    13561 catcgagcct cagcatcgag aactggtaaa aggcgatagc aaatttgtcg tcaacagccg
    13621 tgtcgacgtg aaggtggggc tggatggcgt tgagtttctc ggtgccagcg cctcagaatg
    13681 gattaacggc gggatacgta ttctgccggg cgataaaggc gagatgaaag ccagctatcc
    13741 actgtatgcc aatctggaaa aagcgctgga gaacagcctt agcgatttac ccaccacaac
    13801 catgagtttg agtgcagaga cgctgccgga tgtgcaggca ggatcggtag tgctgtaccg
    13861 taaatttgaa gttggtgaag ttattaccgt gcgtccgcga gctaacgcgt tcgatatcga
    13921 tctgcacatt aaaccggaat atcgcaacct tttgaccagc aacagcgtgt tctgggcaga
    13981 aggcggggcg aaagttcagc tgaatggtag tggcctgacc gtacaggcat ccccgctctc
    14041 cagagcatta aagggggcca ttagcttcga taacctcagt ggtgccagcg ccagtcagcg
    14101 taaaggcgac aagcgaattc tgtatgcttc cgaaacagcg gcccgtgcgg ttggcaggca
    14161 gattacgctt cacgctttcg atgccggaaa actggcggtc gggatgccaa ttcgctatct
    14221 cggtattgat atcgggcaaa tccagacgct ggatctgatt accgcacgca atgaagtaca
    14281 ggcaaaggcg gtgctctatc cggaatatgt ccagaccttt gctcgcggtg gtacgcgctt
    14341 ctcagtggtc acaccgcaaa tctcggcagc tggcgttgag catctagata ctatcctcca
    14401 gccgtatatc aacgtcgaac caggccgtgg caatcctcgt cgcgactttg aattagaaga
    14461 ggccaccatt actgattcgc gttacctaga tggtttaagc attattgttg aagcgccgga
    14521 agccggttcg ttaggcatcg gtacgcctgt gctgttccgt ggtctggaag taggtacggt
    14581 taccggaatg acgctgggga cattgtcaga tcgcgtgatg attgcgatgc gcatcagtaa
    14641 acgctatcaa cacctggtgc gtaacaattc cgtcttctgg ttggcatcgg gttacagtct
    14701 ggactttggt ctgacgggcg gcgtagtgaa aaccggcacc tttaaccaat ttatccgtgg
    14761 cggcatcgcc ttcgccacgc ctccgggtac gccacttgca ccgaaagccc aagaaggcaa
    14821 acacttcctg ttgcaggaaa gtgaaccgaa agagtggcgt gaatggggaa ctgcgcttcc
    14881 caaataatgc ccactgctcc ggcgtgcctg cgccggagcg cttatgctaa actgcgcgcc
    14941 tgtttttttg ccagtggtac atgctcgtgg cccaacacac cgtttatttc ccggacgcgt
    15001 ttctgacaca aatgcgcgaa gctatgcctt cgacgctctc atttgatgat tttcttgccg
    15061 cctgtcagcg cccgttgcgc cgcagcattc gcgttaatac gctgaaaatc tccgttgctg
    15121 atttcctgca attaaccgct ccttatggct ggacgcttac gccaattccg tggtgtgaag
    15181 aaggtttctg gattgaacgc gacaatgaag atgcattgcc attgggtagt accaccgagc
    15241 atttaagtgg cctgttttat attcaggaag ccagttcaat gttgcccgtc gccgccttgt
    15301 ttgctgacgg taatgcacca cagcgggtga tggatgtcgc tgctgcgcca ggctcaaaaa
    15361 cgacgcaaat tgccgcgcgg atgaataacg aaggggcaat ccttgccaat gagttttccg
    15421 ccagttgggt aaaagtgtta catgccaata tcagccgctg tggcatcagt aatgttgcgc
    15481 tcacacattt tggtggccgc gtgtttggtg cggcagtgcc agaaatgttt gatgccattt
    15541 tgctggacgc tccctgctcc ggcgaaggcg tggtgcgtaa agatcccgat gcgctaaaaa
    15601 actggtcacc cgaaagcaat caggaaatcg cagctaccca acgggaactg attgacagcg
    15661 cctttcatgc attacgtcct ggcggtacgc tggtttactc gacctatacc ttaaaccggg
    15721 aagaaaacga agccgtttgc cggtggctga aagagactta ccccgacgaa gtagagtttt
    15781 taccacttgg cgatctcttc cctggtgcaa acaaggcgct gaccgaagaa ggttttttgc
    15841 atgttttccc acaaatttac gactgcgaag gcttcttcgt tgctcgtctg cgtaaaactc
    15901 aggcgatccc cgccttaccc gccccaaaat acaaagtggg caatttcccg ttcagcccgg
    15961 tgaaagatcg cgaagccgga caaattcgtc aggcggctgc aagtgttggc ttaaactggg
    16021 atggaaacct gcgactctgg caacgcgaca aagaactgtg gttgttcccg gtaggcattg
    16081 aagccctgat cggtaaagtc cgattttctc gcttagggat taaacttgcc gaaacgcata
    16141 acaaaggtta tcgctggcag catgaagcgg ttattgccca tgcctccccc gacaatgtaa
    16201 acgcttttga actgacaccg caggaagcgt aggagtggta tcgcgggcgc gatgtttacc
    16261 cgcaagccgc gccagtggcg gatgacgtgt tggttacttt ccagcatcag ccgattggtt
    16321 tagccaaacg gattggttcg cgactgaaaa acagctaccc gcgtgaactg gtgcgggacg
    16381 ggaaactttt taccagtaac gcatgacagc gcacaaaaaa agcgcacttt ttgactggca
    16441 cattcgccgg cctcaactag gctgaaaaat tgtgcgatcg gactggtcgt actacaatca
    16501 gcagctaaat ggagagcacg aagatgaaaa ccagtgtgcg cataggcgct tttgaaatcg
    16561 acgacggcga attacacggt gaatcgccgg gtgatcgaac gttaaccatt ccttgtaaat
    16621 ctgaccccga tttatgtatg caactggatg cctgggatgc tgaaaccagt atccctgccc
    16681 tgctaaatgg cgaacactct gtcctttacc gtacccgtta cgatcaacaa tctgatgcct
    16741 ggattatgcg tcttgcctga tccaaaaaga acccgtcggc atggcgggtt atttgtcctg
    16801 gttattcccc cgttgtaaaa tctctcctaa acttaacggt acggcaccac acttcgggga
    16861 tgaaatgttc gcgctggtac tttttgtttg ctacctggat ggcggttgtg aagatattgt
    16921 tgtggatgtc tacaacacgg aacagcagtg tctttattct atgagcgatc aacggatccg
    16981 ccatggcagt tgttttccga ttgaggattt tatagatggt ttctggcaac cagcacagga
    17041 gtatggtgat ttttaattat tgcagttgca caagagtcag ttagccccca aagacagcac
    17101 cggtatcaat ataatgcagg ttgccaatat ccacgcgatg tcgcaacggt gtatgaccaa
    17161 accagaaatg atcagcacct gtaattccct gcccttttgg gcgttcacct aatcgcgagc
    17221 ggctccacaa gacctgatgc aaatcaacgt ccttttgcca ttcat
//
LOCUS       DADLTY010000087        17089 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.087, whole genome shotgun sequence.
ACCESSION   DADLTY010000087 DADLTY010000000
VERSION     DADLTY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.087"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..508
                     /locus_tag="JN334_002426"
     CDS             <1..508
                     /locus_tag="JN334_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HAY7438538.1"
                     /translation="FREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGF
                     TQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIR
                     HNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQ
                     YSRAWTVK"
     gene            complement(509..1669)
                     /gene="ybdL"
                     /locus_tag="JN334_002427"
     CDS             complement(509..1669)
                     /gene="ybdL"
                     /locus_tag="JN334_002427"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HAY7438539.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1778..2866
                     /gene="hcxA"
                     /locus_tag="JN334_002428"
     CDS             1778..2866
                     /gene="hcxA"
                     /locus_tag="JN334_002428"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HAY7438540.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2876..3073)
                     /locus_tag="JN334_002429"
     CDS             complement(2876..3073)
                     /locus_tag="JN334_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY7438541.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3255..5360)
                     /gene="cstA"
                     /locus_tag="JN334_002430"
     CDS             complement(3255..5360)
                     /gene="cstA"
                     /locus_tag="JN334_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HAY7438542.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5541..5954)
                     /gene="entH"
                     /locus_tag="JN334_002431"
     CDS             complement(5541..5954)
                     /gene="entH"
                     /locus_tag="JN334_002431"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HAY7438543.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5957..6703)
                     /gene="dhbA"
                     /locus_tag="JN334_002432"
     CDS             complement(5957..6703)
                     /gene="dhbA"
                     /locus_tag="JN334_002432"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase"
                     /protein_id="HAY7438544.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6703..7560)
                     /gene="entB"
                     /locus_tag="JN334_002433"
     CDS             complement(6703..7560)
                     /gene="entB"
                     /locus_tag="JN334_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HAY7438545.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7574..9184)
                     /gene="entE"
                     /locus_tag="JN334_002434"
     CDS             complement(7574..9184)
                     /gene="entE"
                     /locus_tag="JN334_002434"
                     /EC_number="2.7.7.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase"
                     /protein_id="HAY7438546.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9194..10369)
                     /gene="entC"
                     /locus_tag="JN334_002435"
                     /pseudo
     CDS             complement(9194..10369)
                     /gene="entC"
                     /locus_tag="JN334_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10661..11617
                     /gene="fepB"
                     /locus_tag="JN334_002436"
     CDS             10661..11617
                     /gene="fepB"
                     /locus_tag="JN334_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY7438547.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11621..12853)
                     /gene="entS"
                     /locus_tag="JN334_002437"
     CDS             complement(11621..12853)
                     /gene="entS"
                     /locus_tag="JN334_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HAY7438548.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12964..13968
                     /gene="fepD"
                     /locus_tag="JN334_002438"
     CDS             12964..13968
                     /gene="fepD"
                     /locus_tag="JN334_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HAY7438549.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13965..14957
                     /gene="fepG"
                     /locus_tag="JN334_002439"
     CDS             13965..14957
                     /gene="fepG"
                     /locus_tag="JN334_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HAY7438550.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14954..15769
                     /gene="fepC"
                     /locus_tag="JN334_002440"
     CDS             14954..15769
                     /gene="fepC"
                     /locus_tag="JN334_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY7438551.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15766..16899)
                     /gene="fepE"
                     /locus_tag="JN334_002441"
                     /pseudo
     CDS             complement(15766..16899)
                     /gene="fepE"
                     /locus_tag="JN334_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3992 a         4863 c         4445 g         3789 t
ORIGIN      
        1 ctttcgtgaa aagccagtgg attgcgttct gtgggttaaa aacagccaac ttatgcctaa
       61 tgattacaac ccaaacaacg tggcaccacc cgaaaaaaaa ctgctgcaaa aatccattga
      121 aatagatggt tttacccaac ccattgtggt cacacatacg gataaaaatg ccatggaaat
      181 cgtggatggt tttcaccgcc atgaaattgg taaaggctca tcatcattaa aattacgctt
      241 aaaaggttat ttgccggtga cctgtctgga aggtacgcgt aatcaacgca tcgccgcgac
      301 tattcgtcat aatcgcgctc gtggacgcca tcaaatcacc gccatgtcgg aaatcgtccg
      361 tgaacttagc cagttgggat gggatgataa taaaattggc aaagagctgg gaatggacag
      421 cgacgaagtg ctgcgactga agcaaattaa cggcctgcaa gagttgtttg ccgaccgtca
      481 atattcccgc gcctggacgg ttaaatagct aaagctggcg caggcgttca gctgctgcca
      541 gcaacgtcga ttccttcttg gcaaaacaga gacgaatcag tttatgtggg aagggatcgg
      601 cgcaaaacac cgacagcgga atcgccgcta cgccgtgctc ctgcgtcagc cactggcaaa
      661 actcaacatc atccagggta gaaaccgcgc tgtaatccac cagcaaaaag tatgtacctt
      721 cacacggtaa aatctccagc cggctttcat ttaaagcatt caccagaata tcgcgcttct
      781 ggcgataaaa gtccggtaac gcaagataat gctcaggttc tgcacgtagc atatcagcaa
      841 gcgccagctg tgccggggta ttcaccgaaa aggtcagata ctgatgtacc ttgcgaattt
      901 cggcgctgat gggcgctggc gcaacacaat aacccacttt ccagccggtc atatgatagg
      961 tcttgccaaa tgaagaaacc gccactgccc gctcacgcag ctgcggatgc gccagcacac
     1021 tggcatggcc ctgttgtgaa aagttgatgt gctcgtagac ttcatcgcta atgacaaaaa
     1081 tctcgtgccc ggcgatcgcc tgccacaaag cggcgaaatc agcctgctgc cagacagttg
     1141 cactggggtt atgcggagtg ttgaggatca ccagtctggt gcgctcgctt aataatgcgg
     1201 caaattcctg ccagtcaacg cgaaaatgcg gtggttgcag tgccatacgt ttcactattc
     1261 ccccagaaag cgcgatggcg ggggcgtaac tgtcatagct gggatcaaaa caaatcactt
     1321 catcgccatt gcgcaccagt gcggtaatcg ccgcgtataa cgcttccgtc gcccctgccg
     1381 ttacggtgat atcgctatcg gcatctggtt gatagccata caaacgttcc gttttctgag
     1441 caatcgcctc gcgcaaggcc tgcacgccgg tcatgggcgc gtattggttt gccccctggg
     1501 caacgtggta cgccagccgc tcctgtaaat agcgcggacc atcaaaatca ggaaagcctt
     1561 gcgacaggtt aatcgcctgg tgttgctgcg ccagcgcgct catctgggtg aaaatagtgg
     1621 tgccaagttg tggaagtttg ctttgtggaa tcagagggtt atttgtcatt tttattgtac
     1681 ctgacgaagt tgcgtgttga aggcactata acacgatgcc agtatttggc aatcaagacg
     1741 tttagatgtc taaataaaac aataaggaca acacaacatg cctcacaatc ctatccgcgt
     1801 ggtcgtcggc ccggctaact acttttcaca tccaggaagt ttcaatcacc tgcacaattt
     1861 tttcactgat gaacaacttt ctcgcgcggt gtggatctac ggcaaacgcg ccattgctgc
     1921 ggcgcaaacc aaacttccgc cagcgtttgg acttccaggg gcaaagcata ttttgtttcg
     1981 tggtcattgc agcgaaagcg atgtacagca actggcggct gagtccggtg acgatcgcag
     2041 cgtggtgatt ggcgtcggcg gcggcgcact gctcgacacc gcgaaagccc tcgcccgccg
     2101 tctcggtctg ccatttgttg ccgctccgac gatcgccgcc acctgcgcag cctggacacc
     2161 gctctccgtc tggtataacg atgccggaca ggcgctgcat tatgagattt tcgacgacgc
     2221 caattttatg gtgctggtgg aaccggagat tatcctcaat gcaccgcaac aatatctgct
     2281 ggcggggatc ggtgacacgc tggcgaaatg gtatgaagcg gtggtgctgg ctccgcaacc
     2341 agaaacgttg ccgctaaccg tgcggctggg gatcaataat gcgcaggcca ttcgcgacgt
     2401 cttgttaaac agtagcgaac aggcacttgc cgatcagcaa aatcagcagt taacgcaatc
     2461 attttgcgat gtggtggatg ctattattgc tggtggtggg atggtcggtg gtctgggcga
     2521 tcgttttacg cgtgtggcgg cggctcatgc cgtgcataac ggtctgactg tcctgccgca
     2581 aaccgagaag tttcttcacg gcaccaaagt cgcttacgga attctggtgc aaagcgcctt
     2641 gctgggtcag gatgatgtgc tggcgcaatt aaccggggcg tatcagcgtt ttcatctgcc
     2701 gactacgctg gcggagctgg aggtggatat caataatcag gcggagatcg acaaagtgat
     2761 tgcccacacc ttgcgcccgg tggagtccat tcattacctg ccagtcacgc tgacaccaga
     2821 tacgttgcgt gcagcgttcg aaaaagtgga atcgtttaaa gcctgacgtg caaatttagc
     2881 agcagcgcgc gccgcctttt ccaccgtagc gcgcgtcctg ccgctcacgg aaaaactcct
     2941 cgtaggtcat cggcgtttga tcgggatgat taacccgcat atgttcgaca tagttgtcgt
     3001 aatcaggcat accaatcatc agcttcgccg cctgacctaa atattttccg gcttttgcca
     3061 gtgaatcaaa catcttggcc tccgctaacc atgttgctga aagagtgccg gatgcggcgt
     3121 gaacgcttta tccggcctac aaatgctatc aacgcactcg gtcagtcccc tcgccccatc
     3181 ggggagaggg ttagggtgag ggggaaccct cggcactatg ccaacattcg ccaacatccc
     3241 ccctcactct gactttagtg cgcgcctttc gcctgcgcta cgatctcctc gacattttcc
     3301 ggcatcggct catacggcgt ttctttcgcc gttggcttcg gatctttcaa tgccgcaagt
     3361 gccgtcttaa tcgagaacag tgccaggacc accacgacca ccataaagaa gatggttaac
     3421 ccggcatcca gacggttgtt gaacaccagt tgcgccagct gtgactcagt atactgcgac
     3481 ggaatattgc cgctgtcgat cattgcctgg aacttgttag caatgaccag gaagccgact
     3541 ttcgcatccg ggctaaacgc tttcagccag tcagcggtca gggtacaaat cagcagccag
     3601 gccgttggta ccagcgccac ccaggcgtaa cgttgacgtt tcatcttgaa caacaccacg
     3661 gcacagagca tcagcgccat ccctgccagc atctggttgg caataccaaa cagcggccac
     3721 agagtgttaa taccgcccag cggatcgacc acgctctgat ggaggaagta gccccacgcc
     3781 agtacgcaca gcgctgttgc cagcaggtta gcaggcagtg aatcggtccg cttcaggcca
     3841 ggagacacca cgcccagcag atcctgcaac ataaagcgcg cagcacgcgt acccgcatct
     3901 accgccgtca gaataaacag agcttcaaac agaatggcga agtgatacca gaacgccaca
     3961 tccatcatac cacccagcgc accgtgcagg atgtaagcca tccccaccgc caacgtcggc
     4021 gcaccgcccg cacgggaaat gatcgactgt tcacccactt cgctggcaat ctggttgagc
     4081 gtatctggtg taatgctaaa gccccagcta ctcaccacct gcgcggcaga agcgactaca
     4141 tccgccgtcc ctgccggagc cagcaccgcc attgggctgt tcatggcaaa atacacgccc
     4201 ggatcgatga tacaggcaga aactagcgcc ataatcgcca cgaaggattc catcaacatc
     4261 ccaccgtagc cgataaagca cgcctgccct tcgttcgcca gcatcttcgg cgtggtgccg
     4321 gaagagatca gcgcatggaa gccagacacc gcaccacagg cgatggtgat aaacaggaac
     4381 gggaacaggt taccggtcca taccgggcca gtgccatcga caaatttggt cagcgcaggc
     4441 atggtcagcg tcgggcgcat aatcaaaatg cctaccgcca gaccgacgat agtcccgatt
     4501 ttcaggaagg tcgagagata gtcacgcggg gccagcagca accacaccgg cagcaccgcc
     4561 gccacaaaac cgtagcccac cagcatccag gtcagctgca cgccggtaaa gtcaaagtac
     4621 ggtgcccagg tcggactttc tgccacccag ccgccagaga taatggcgaa aatcaggaac
     4681 accaggccaa tcactgacac ttcgccaatg cggcccggac gcagatagcg caggtagatt
     4741 cccataaaaa tcgccagcgg aatggtgaac gcaacggtat acgttcccca cgggctatga
     4801 gtcagggctt tcaccacgat catcgccagt actgcaagga taatgaccat gatcataaag
     4861 caggctacca gcgcaatcac cccggcagtt ggccccatct cttctttgac cagctcaccc
     4921 agcgagcgac cgtcacggcg cgtagaaaca aacagcacca tgaaatcctg caccgcaccg
     4981 gcgagaacca ccccggcgag cagccagatc atccccggca ggtagcccat ttgcgctgcc
     5041 agcaccggcc ccaccagcgg ccctgctccg gcaatggccg caaaatggtg accgaacagc
     5101 actttcttgt ccgtcggcac atagtccagc ccgtcgttat ggcgcaccgc tggcgtcata
     5161 cgcgtcgggt caaccgccag cacatttttg gcgatataca gcccataaaa acggtaagcg
     5221 atcagataga tacagaccga cgccaccaca atccacagcg cgttgatctg ttccccacga
     5281 tttaaagcaa tgtatcccag agcaaatgct cccattacag agagcactgt ccagacgagg
     5341 tatttccctg atttgttcat agttgttatc cgtgtgcgtg tccatagaga tgttacattt
     5401 tgtttctata acatctttgt acgatttaac aacacggtaa ccatgtaaat cggagttgaa
     5461 accaatattt aacttaacaa tgttaactcg atcactccgt taaccgagtt ttcctgcaat
     5521 ctcatttaat tctgtcgggc tcatcccaaa actgccgtac ccagccgaca agtgcagcaa
     5581 cgccgcccct gttcatcgaa aacgacgatt tcccaactct gattttgccg cccaagatgc
     5641 agcggctggc agacgccgcg taccttgcct tcagacaccg ggcgatgatg ggtggcgtta
     5701 agttccgtgc ccaccacaca ctgcccgtca cgggtcatca taaatccggc catcgatccc
     5761 agcgtttcag ccagcgccgc cgacgcgccg ccatgcagca ggccaaacgg ctgatgagta
     5821 cgggtatcaa ccggcatttc ggcttccagc acatcatctc ccagaagggt atacacaatt
     5881 cccagatgcg ccaccattgt gttatcgctg gtggcgttca gttcgtcgag cgttaaatgg
     5941 cgtttccaga tcatgcttat gcccccagcg ttgagccgcc atcgaccaca atatcctgta
     6001 gggtgatatg gctggcgagg tcagaggcga ggaacaaaat cgtgttggcg atctcttgtg
     6061 gacgggcgat tttccccagc ggaatgccga gtttaaactg ctcgccaaag ccgcgaatac
     6121 gctgttcttc ggcgtcatcg ctcacccaca gcgtgcgttg catatcggtg tcggtggagc
     6181 caggcgaaac cacattacag cgcacgccgc tacccgccag ttccagcccg acgctcaacg
     6241 ccaggctttt cagcgccgct ttcgatgcgc cataagcact cataccaata cgcgccgtgt
     6301 gtgcggcgtc ggacgccaca gtgacaatcg ccccgccccg ctgacggcga aactggttca
     6361 tggtttgctg gaacaggtta aacgcaccgc cgacgttaac cgcaaaagtc tgctgccagt
     6421 cctctttact gagctgatcg gtcgcgccca tgcgcaaaat tcccgccgca ttgatcagca
     6481 cgtccagtcg ctccgtttca gctaacagtc gctgacatac ctgcgccacc tgcccggcgt
     6541 cggcaacatc cattacttcg gtcgcaaagg gatattgctc ctgagtgaac gcttgatcaa
     6601 aacctgtaac tttcgctccc gcctcaacaa acgtcagcgc cgtggcgtag ccgatacctt
     6661 tacctgcgcc ggttacccag acatttttac cgctgaaatc cattatttca cctcgcggga
     6721 gagtagcttc caccaggcgt cgatggtcgg gtttttcgcc agcatgacaa agtcgatatc
     6781 accatgcact ttgcgccagc gcgccgccag cgccatcatg cgcaccgaat ccagaccgta
     6841 gtcgatcagg ttgtcgtcat cgaacggttc atcggactcg tccagcaacg gcaggatcac
     6901 ctcacgcagc gccgctttgc tggcggggat aggtgctggc agtaattctt cggtcatcac
     6961 cacccggcca gaacgtccgg ccacatattt cagcgacatc aaatgctcgt cacggctgaa
     7021 atcagccagc gcatccgcca ccataaacgg tttaatatcg cgcataaatg cgtcggttgc
     7081 ggtggtcata cagccaatgt gggcatatac tcctgtaata atcagctggt tacgtccact
     7141 ctctttgagc atttgctcca gcggagaacg atgaaacgcg ctgtagcgcc acttcaccag
     7201 caccgtgtcg tcggcatctg gcgtcaggcg atccaccacc ttttgctgtt ccggcgagcg
     7261 ggtcaggccc ggcccccaca tatcattcaa cagcgcccga tcttcatcgc tctgctcttt
     7321 cggctgggcg gtgtaataaa ccgggatatt gtgctgtttg cagtagtcgc gcagcgcagc
     7381 aatattcgcg atcacctgct ccatcatcgg gcagttctcg ccccagaagc tgacaaaata
     7441 gtcctgcata tcatggatta acaacgcggc acgttgcggt tcaaaggccc agtcaacttt
     7501 attctgcgga atatcgtgag actccggcag tgcgtaagcc tgtaattttg gaatagccat
     7561 cgtgttctct ccttcaggct gatgcgcgtg acgccagcca ctgacgtaat tgttttttat
     7621 cgactttccc gaccgccgtc agcggaagtg aatccacaca ctccacgcga tccggtaatt
     7681 taaattcggc aataccctgt tcacgcagga aacgacgcac ctgcaccgcg cgcagcggct
     7741 cttttaccac cagataagca cagctttttt cgcccatcag ctcattttcc atgctcacca
     7801 gtgcggcata gatcactgcc ggatggctca gcagcaggtt ttcgatatct tcggcagcga
     7861 tcttctcgcc gccacggttg atctgatctt tctcgcgccc ctgcacggtg atgtaaccct
     7921 ctggatcaat agagatcaga tcgccggaac agtaaaaacc gttggcatca aaggcactgg
     7981 cattgtgctg cggacttttg taatagccac ggaaggtgta cggcccgcgc gtcatcaggc
     8041 gtccaacttc cccttgcggc agtggatttc cttcggcatc ggcaacccac acttcatcgt
     8101 ccggacacat tgggtaaccc tgggtatgga taattttctc cgcgctatca tcaagacggg
     8161 tgtagttcac cagcccttcc gccatgccaa acacctgctg caactggcag ccaatctcag
     8221 cgggaatacg cgctgcaagc gtggcagaaa gacgtgcgcc gccgacctgt aacagtttca
     8281 gcgaggcaag ctgcgcccgg ctttcgcctt cagccagcgc ctgcaaccac aggctgactg
     8341 ccggcggcac cagcgcggta acgttaacct gatgttttgc aatcaacggg aagcaaagcg
     8401 tggcgctggg atcggccgcc agaacaaccg ttcctccggc aagaaagacg cccagcgatc
     8461 ctggcgaact catagtgtag ttatgagccg ccgggatcga gcacaggtag cgcgtctgtt
     8521 gtgtgaactg acaaatctcg gcgctacgac gcacgctgta gtaatagtcg ttatgagtgc
     8581 gcgggatcag tttcggtgtg ccggtggtgc cgccggaaag ctggaaatag gccacttcat
     8641 cagcaggtga tggcgtggca gtaaaaccct ctgccggatg gttaatcgca tcctgcaagt
     8701 tatgctcacc gctgtcgttg tgcagttgca ccacgcgaat ggaagaatgc tctgcaacaa
     8761 atgtgttgag gaaatcatcc ccgctaaata gcgcatgctg gcgatcggca atcagtaacg
     8821 ccggttcaat ctggctggcg taggcgttca gttcactacg ctgatggctg aacagcgcca
     8881 gcaccggcgc gacgcccagt ttcagcagcg cgaaaaaggt aatgtaaagt tcagcgacgt
     8941 tacccagttg taccagcgcg gtttcaccag gtttaatgcc ctgacggcgc aaactacagg
     9001 cgaggttatc cgccgcctga ttcagctccc gataactcaa ctgccgctcg ccgtcgataa
     9061 ccgcgatgct gtcactcgca gcgtggcgag tcagaatgtc ggtcagtggc aaatcctgcc
     9121 agtagccttt ttcccgatag cgacgggcaa actcttccgg ccagcgggtg aatggaatgc
     9181 tcatcctcgc tccttaatgc aatccaaaaa cgttcaacat ggtagaaagt ttgacgcctg
     9241 tttcgcgcca ctcacccaac ggtgacgacg caggcacaat ccccgctccg gcaaacagac
     9301 gcacctgatt ttcccgcagc ttcgcgcagc ggatggtcac cacccattcg ccgttacctt
     9361 cgctgtcaca ccaacccaca atgccgccaa acagttcgcg gtcgaacggc tccagttcag
     9421 caataacctg ggtcgcggcc tgatgcggga aaccgctcag cgcgggggtc ggatgcagca
     9481 gacaggccag agtcagtgcg ttttcttgcg aattcgcttt accttcaaag ggagttgcga
     9541 gatgccacag cgtcggcgtg gtaatcaatt gtggagagga gggaacgtgt aactcactac
     9601 tgcgttcgcg cagtacctct ttcatcgcct gagtccccag ttcatgttca tggcgatctt
     9661 tttctgacgc cagcagacga ttacccgctt cgcgatcgag cacttcatcc ggctgacgac
     9721 gcgcggaacc ggctaacgga atggagctaa aacgctcgcc gtctttacgt agcagcagtt
     9781 ccgggctggc ccccagcagg acgccaccat cagccagcgg gacatggaag ttgtaactaa
     9841 ccgggttttg cgcaatcaac cgttccagca atacgccact atcaatggcg gcgtcagtgg
     9901 tgatatcaat caatcgtgac aacaccactt tgtcgacctg cggcgtggcg gtaagtgcgg
     9961 cggcgcgggc acccatctgt tcaaacgtgg tttgttcagg aattgcctgg cgttccacca
    10021 cgttcagcga ctggctgcgg gtaaaacagc gggctgaggt ctgtttttcc tgacgggaga
    10081 acgactgcta ggattcggga atatacagcg acgaaggctg acgtggatcg aagggaatcg
    10141 ccccgaccat caccggattt ttgatgccct gcgctttggc atcggcaaac agcgcggcga
    10201 gtttttgctg gaagggactg tcgggcgaat ccccgttcac agccggttca tcgaaacggg
    10261 cgaaacatcc tgacgtcgta aaactgcggt acggcgacat aaagaaaaag cgattgggcg
    10321 caagtgttgc catggtctgc tgtacttcct cagccagtga cgtatccata tcatcctcca
    10381 caaaatgata aaggctttaa taatgattat cacttatatt ttcggccgct aacctaaaag
    10441 caaacgcgca ctatgtcaac tcttgaggta acgcaatctg caactcgggg ttgcatctgg
    10501 ttgtggttac aatgaaaatg agaagcatta ttgatgaatt cgcataagcg caatgtgatg
    10561 tcctgcgccg ttttgccccc tctccccaaa gggacggatt gtgcgctttg tcgaatttgt
    10621 cattacgccc ttaaccttat taataacagg aagctgactt gtgagactcg ccccgctcta
    10681 ccgcaacgcc cttctattaa caggactttt gctttcagga atagccgtag ttcaggccgc
    10741 cgactggccg cgtcagatta ctgacagccg gggcactcat accctggaaa gccagccgca
    10801 gcgtattgtt tccaccagcg tcaccctgac cggatcactg ctggcgattg atgctccggt
    10861 gatcgccagc ggcgcgacca cgccgaataa ccgcgtcgca gatgaccagg gctttttacg
    10921 ccagtggagc aaggtggcga aagaacgcaa actgcaacgg ctctatatcg gcgaaccgag
    10981 cgccgaagcc gttgccgcgc aaatgccgga tctgatttta attagcgcaa ccggcgggga
    11041 ttcggcgctg gcgctgtatg atcagctttc caccatcgcc ccgacattaa tcatcaatta
    11101 cgacgacaaa agctggcagt cgctgttaac gcagcttggc gaaattaccg ggcatgagaa
    11161 acaagcggca gagcggattg cacagtttga taagcaactg gcagcggcga aagagcaaat
    11221 caaattaccg ccgcagccgg tcactgccat tgtctatacc gccgctgcac acagtgccaa
    11281 tctctggacg ccggaatcag cacaagggca gatgctggaa caactcggct ttacgctggc
    11341 gaaattgccc gcagggttaa acgccagcca aagccagggc aaacgccatg acatcattca
    11401 gcttggtggg gaaaatctgg ctgcggggtt aaacggtgaa tcgctattcc tgttcgctgg
    11461 tgatcagaaa gatgccgatg cgatttatgc caatccactg ctcgcgcacc tgcctgcggt
    11521 acaaaacaag caggtttatg cgctgggaac cgagacgttc cgcctggatt actacagcgc
    11581 catgcaagtg ctggaacggc ttaaggcgct gttttaagca ttaactgtcg gacgctgtca
    11641 cctgcggcgg cgtctggcga aaacgtcgca actccaccag caccagcaat aacaacacgc
    11701 cgataatcaa caaaccaaaa ccgctcgcgc ttgcggaagc aaccggtgtc atcatcgcgc
    11761 ccaaaccacc cagcagcgcc gcgcctatgg catcgcccgt cacgttctgc gccgtccaca
    11821 aaccgttaat ccgccctaac atcgcttccg gggtttgcgt ttgcagcatt gtatattgca
    11881 gcaacgaact aaccgcgctc agccagccga acagcgccag acaaaccacg cctaaaatcc
    11941 acatcggcat caggccaaac agaccaatgg cgaggaacga tcccagcgtg gagagcagca
    12001 tcaataaccc tggtcgcgca ctatgtgcca gcttcccgct ggttaacgca ccaatagccg
    12061 cgccgagcgg gatcgccgcg tagagaaaac caatctgtgc cgctgacatc tgccagttgt
    12121 cagccagcgc cggatacagt accagcaccg cgctcgccat cgtcaataaa ccacccagca
    12181 gcgggctggc aagcagaaaa cgaaatcctg ccagtaatga tttcaacgga tgctcaagcg
    12241 gctgcggtgg cggtggcaac tccggaaggc ttaataacgg taacaaggta ataaacgtgc
    12301 ccgccgccgc cagcccgtag ttccaggcta cgccgccggt cgccagcaat aaaccgccaa
    12361 tcatgggcga aatcaccgac cccaggcgca cggtcaacat ggtgatcgcc ccggcctgca
    12421 ttaagttttc acgccctacc agtgcagatg ttgccgccag tagcgccgta acaccaagtg
    12481 atgcgaaaaa accatcccat aaaccaagta aatagattgc cagcaatgac ggctccggca
    12541 gcagtgcatt aaggcacagt ccaatgaagc caatgccaca ggtgccgcgc gccagcaaaa
    12601 tcactttttt gcgttcatag cgatccgcca gcacaccgcc aaccatcagg ccaacaaaca
    12661 tcgcgccgcc ggtcagcgtc accgaaagcc ccacctgcca ggtagaatgg gtcatcatct
    12721 ggatctgcac cggcaccgcg acgccgagca aacccagaga cacaattgag ataaaacgag
    12781 cgaggaatac tgcgcgaaac gccgggtgcg ttttcaacag gctgaggtta agcagccagg
    12841 attgtttatt cattacaatg ccttgccatc aaatatttta aatacctatt cttaggctgc
    12901 acatgttaac atatccaaat aagatcgata acgataatta atttcattat catggaagtt
    12961 cgtatatctg gttctgttgc cgtgacacgc gccattgccg tgcccggatt gctgttatta
    13021 ctgattatcg cgacggcatt aagcctgctc attggggcaa aatcactccc cgcttccgta
    13081 gtgctggagg ccttctccgg cacctgccag agcgccgact gcaccatcgt gctcgacgcg
    13141 cggctgccgc gtacccttgc cggtttactg gcaggcggcg cgcttggcct tgccggggcg
    13201 ttaatgcaaa ccctcacccg aaacccactt gccgaccccg gcttgcttgg cgtgaacgcc
    13261 ggagccagct ttgccattgt gctgggtgcg gcgctgtttg gttactcatc cgcacaggaa
    13321 caactggcga tggccttcgc cggggcgctg gtggcctcat tgattgttgc ctttaccggc
    13381 agtcagggtg gagggcagtt aagtccggtg cgtttaaccc tggcgggcgt ggcgctggcg
    13441 gcggtgctgg aaggactgac cagcggcatc gccctgctta atcctgacgt ctacgatcaa
    13501 ctgcgtttct ggcaagccgg ttcgctggat attcgcaatc tacatacctt aaaagtggtg
    13561 ctgatcccgg ttctgatcgc cggagcaact gcgctattac tgagtcgcgc gctgaacagt
    13621 ttgagcctcg gcagtgacac cgcgacggcg ctgggcagtc gcgtggcgcg cacacagttg
    13681 attggtctgc tggcgattac cgtgctttgt ggtagtgcga cggcggtagt tggcccgatt
    13741 gcctttatcg gcctgatgat gccgcacatg gcgcgctggc tggtaggtgc cgatcatcgc
    13801 tggtcgctgc ccgtcacgct acttgctacc cctgccctgc tgctgtttgc cgatatcatc
    13861 gggcgggtga ttgtgcccgg cgaactgcgc gtttctgtgg tcagtgcgtt tattggtgca
    13921 ccggtgctga tcttcctcgt gcgacgtaaa acgcgaggtg gtgcatgatt tacgtctctc
    13981 gccgattact catcacctgt ttgctgctga tttccgcctg tgtggttgca ggtatctggg
    14041 gattacgcag cggtgccgtc acgctggaaa tctcgcaggt attcgccgcg ttgatgggcg
    14101 atgcgccgcg cagtatgacg atggtggtca ccgaatggcg tttaccacgc gtgctgatgg
    14161 cgctgttgat tggcgcggcg ctgggcgtca gcggcgcgat ttttcagtcg ctgatgcgta
    14221 acccgctcgg cagccctgac gtaatgggct ttaacaccgg ggcgtggagc ggcgtgctgg
    14281 tggcgatggt gctgtttggt caggatctga cggctatcgc gctggcagca atggtgggcg
    14341 gcattatcac ttcgctgttg gtctggctgc tcgcctggcg taacggtatc gacacctttc
    14401 ggttgattat tatcggtatc ggcgttcgcg ccatgctggt ggcctttaat acctggctgt
    14461 tgctgaaagc gtctttagaa acggcgctaa cagcaggttt gtggaatgcc ggatcgctca
    14521 acggcctgac gtgggcaaaa acctcgcctt ccgcacccat cattatattg atgctcattg
    14581 ccgccacctt gctggtgcgg cgaatgcgct tgctggagat gggcgatgat accgcctgcg
    14641 cgctgggcgt cagcgtcgag cgttcgcgtc tgttaatgat gctggttgca gtggtgctta
    14701 ccgctgcggc aacggcgctt gccgggccga tttcctttat tgctttagtc gcgccgcaca
    14761 ttgcccgacg cattagcggc accgctcgct gggggctgac ccaggcggcg ctgtgcggag
    14821 cgctgttact gctggcggcc gttctctgcg cccaacaact gtttatgccg tatcaacttc
    14881 cggttggcgt cgttaccgtc agcctcggcg gtatttacct tatcgtcttg ttaattcagg
    14941 agtctcgcaa aaaatgaccg aatcagtagc ccgtttgcgc ggcgaacagt taaccctggg
    15001 atatggcaaa tataccgttg cggaaaatct gactgtagaa attcctgatg gtcacttcac
    15061 ggcaattatc ggaccaaatg gctgtggtaa atccacgtta ctgcgtacct taagccgcct
    15121 gatgacgcct gctcacgggc atgtctggct ggatggcgag cacattcaac attacgccag
    15181 taaagaagtt gcacgccgga ttggtctgtt ggcgcaaaat gctaccacgc cgggcgatat
    15241 caccgtgcag gagctggtgg cgcgtggacg ttatccgcat caaccgctgt ttacccgctg
    15301 gcgcaaagag gatgaagaag cggtaacgaa agcgatgcag gccacgggaa taactcatct
    15361 ggcagatcaa agcgtggata ccctttctgg cggacaacgc cagcgagcgt ggatcgcgat
    15421 ggtgctggcc caggaaacgg caattatgct gctcgacgaa cccacgacct ggctggatat
    15481 cagtcatcag attgatctac tggaactgtt aagcgaactg aaccgcgaga aaggctatac
    15541 cctggcggcg gtgctgcacg atcttaatca ggcctgtcgt tacgccagcc atttgattgc
    15601 attgcgggaa gggaaaattg ttgctcaggg agcgccgaag gagattgtca ctgctgaact
    15661 gattgagcgc atttatggtc tgcgatgcat gatcattgac gatccggtag ccggaatgcc
    15721 gcttgtggtg ccgctcggac gaacggcacc ttcaaccgca aatagttaaa ctaagtggtc
    15781 tgccatcatg gcatcctgtt ttctggatgc catcgcatag cgcaataaca cgctaccaca
    15841 agccaccatc ccgccgatca gcgcggaaag gatcacaata atcgccttac ccggaccgtc
    15901 ttttttcact ggcaatgacg ggcttaactg atatttaaac ggcgtaaaat tcacatcgtt
    15961 gacatttgct tttgttaatt gctcgacaag atactgccga ttacgtaatt caccgttcag
    16021 ttccgcaacg tcagtaaccg ctttttcaat ttccagtttg cgttcaatac cgtctgcgcc
    16081 gagggaaata gagaaatcgg ggtcatcttt aacggcctga ccattactgt atacgggctt
    16141 tttaattcct gccgcgttgg caatgtcgag tgaataattg aggcgctgaa tgtttgcatc
    16201 aagttgattt ttcgttttaa tgcgatcctg agccagtttt tctttttcaa actgggtttt
    16261 gatctccagt ttattacgga cgttttctat cgactctttc accaccaacg cagagatata
    16321 atcgatatac ccgctcaaaa cggtctgcgc ctctttactg gttggcgcgg taaaacttag
    16381 cgtccaggat gtatacagtg acggttcatc ttttttctta ctagcattgt catcaaccgc
    16441 tttcattttt tcgctcaatg caacaatagc gcgatgcaaa tccagttcat cgattttcgc
    16501 ctcttttaat tagtccatca cataaggtga tgaacgcagg tactcttcca gcaagctaac
    16561 agactgaaac ttcttgataa acaggttaaa tgcaccgcca cggtcgatat taacatcgag
    16621 atcaagaacg cggagtttgg taaaggtttt ctccaggtcc tgccactgaa tggcttcggc
    16681 gggagtaata acggctgagc tggtccattt ttgcggcagg atgaaagaga tcagtaaacc
    16741 tgcgcaggca aatgcaaaaa cgaccgccat gaccgttttt ttggcccgcc ataaaacttc
    16801 gattagatta agtaaatcaa tttcattatt actgggcgac gccagaggat actcagaaaa
    16861 atgagcgtca cttccctgtt taatattcaa tgatgacatg gggtataacc tgaaaatgag
    16921 tccaataggg gaaaatctcc acggcgctca ctttaccagt tggtgagaat tatccgaagc
    16981 tgaagtttgt aaattccttc cactgaatgc aacattcact ctatttaaga gaaatattca
    17041 tcaaacctat aaatcaaaca aataaaaaac ccataattat tttctgtaa
//
LOCUS       DADLTY010000088        16668 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.088, whole genome shotgun sequence.
ACCESSION   DADLTY010000088 DADLTY010000000
VERSION     DADLTY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.088"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..1166)
                     /gene="emrB"
                     /locus_tag="JN334_002442"
     CDS             complement(<1..1166)
                     /gene="emrB"
                     /locus_tag="JN334_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HAY7438552.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSG"
     gene            complement(1183..2355)
                     /gene="emrA"
                     /locus_tag="JN334_002443"
                     /pseudo
     CDS             complement(1183..2355)
                     /gene="emrA"
                     /locus_tag="JN334_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            complement(2482..3012)
                     /gene="emrR"
                     /locus_tag="JN334_002444"
     CDS             complement(2482..3012)
                     /gene="emrR"
                     /locus_tag="JN334_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HAY7438553.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            2983..3084
                     /locus_tag="JN334_002445"
     CDS             2983..3084
                     /locus_tag="JN334_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438554.1"
                     /translation="MFNGRKRTIHLGMTSLISADIVTTVTNKVNVLF"
     gene            complement(3103..3438)
                     /gene="ygaH"
                     /locus_tag="JN334_002446"
     CDS             complement(3103..3438)
                     /gene="ygaH"
                     /locus_tag="JN334_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HAY7438555.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(3428..4165)
                     /locus_tag="JN334_002447"
     CDS             complement(3428..4165)
                     /locus_tag="JN334_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAY7438556.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(4289..5468)
                     /locus_tag="JN334_002448"
                     /pseudo
     CDS             complement(4289..5468)
                     /locus_tag="JN334_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(5660..6652)
                     /gene="proX"
                     /locus_tag="JN334_002449"
     CDS             complement(5660..6652)
                     /gene="proX"
                     /locus_tag="JN334_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HAY7438557.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(6710..7774)
                     /gene="proW"
                     /locus_tag="JN334_002450"
     CDS             complement(6710..7774)
                     /gene="proW"
                     /locus_tag="JN334_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HAY7438558.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(7767..8969)
                     /gene="proV"
                     /locus_tag="JN334_002451"
     CDS             complement(7767..8969)
                     /gene="proV"
                     /locus_tag="JN334_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HAY7438559.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(9324..10283)
                     /gene="nrdF"
                     /locus_tag="JN334_002452"
     CDS             complement(9324..10283)
                     /gene="nrdF"
                     /locus_tag="JN334_002452"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAY7438560.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(10293..12437)
                     /gene="nrdE"
                     /locus_tag="JN334_002453"
     CDS             complement(10293..12437)
                     /gene="nrdE"
                     /locus_tag="JN334_002453"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAY7438561.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(12410..12820)
                     /gene="nrdI"
                     /locus_tag="JN334_002454"
     CDS             complement(12410..12820)
                     /gene="nrdI"
                     /locus_tag="JN334_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAY7438562.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(12817..13062)
                     /gene="nrdH"
                     /locus_tag="JN334_002455"
     CDS             complement(12817..13062)
                     /gene="nrdH"
                     /locus_tag="JN334_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAY7438563.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(13409..13639)
                     /locus_tag="JN334_002456"
     CDS             complement(13409..13639)
                     /locus_tag="JN334_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438564.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            13791..14135
                     /locus_tag="JN334_002457"
     CDS             13791..14135
                     /locus_tag="JN334_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HAY7438565.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(14172..14621)
                     /gene="alaE"
                     /locus_tag="JN334_002458"
     CDS             complement(14172..14621)
                     /gene="alaE"
                     /locus_tag="JN334_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HAY7438566.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            15289..15693
                     /gene="stpA"
                     /locus_tag="JN334_002459"
     CDS             15289..15693
                     /gene="stpA"
                     /locus_tag="JN334_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HAY7438567.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(15740..16264)
                     /gene="ygaP"
                     /locus_tag="JN334_002460"
     CDS             complement(15740..16264)
                     /gene="ygaP"
                     /locus_tag="JN334_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HAY7438568.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(16274..16573)
                     /locus_tag="JN334_002461"
     CDS             complement(16274..16573)
                     /locus_tag="JN334_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7438569.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT         4252 a         4213 c         4211 g         3992 t
ORIGIN      
        1 ccagacagcg taatggtggt cagcggcata aagaagcaga ccaccgcaaa cccctggata
       61 aactgcggcc aggccgacgc gcaaaaatcc atacctggtt caaaggtata ggcacgccag
      121 tagaagcaga cggcatacat aataaagctg aaggttacca gccgccgcat atccagttta
      181 tgcgcgaagc ggccgataat cggcgacaga atcaccggaa taatccctac cggcgcagag
      241 gccaaacctg cccaggtcgc cgtgtaaccg tagacctcct gcaacaactg cggcagcaga
      301 acaatagcgc cgaagtagag catatacgcg aggctgatac acaagcagcc gatggtgaag
      361 ttgcgcgact taaacaacga cagatcgact atcgggttat cgtcggtcag ctcccagaca
      421 atcaggaagc agatagccac caccgccacc acggtaagaa tgataatttc ctgtgatgaa
      481 aaacagtcca gctctttacc gcggtcgagc ataatctgca ggctgccgat accaataacc
      541 agcagtgcca gccccacggc atcaatccgc cgccgttcgg tgcgggtttc acgtccgcgc
      601 agggtttgca gtgtcatcaa caccaccgcc acgccaatcg gcacgttgat gaagaatatc
      661 cagccccagt ggtaattatc gctgatataa ccgccgagga tcgggccgca aattggcgcg
      721 acaatcaccg tcatcgacca caacgccagc gcgatcgagc gtttggctgg cgggtagtta
      781 ttcagcaata gactttgcga aagcgggatc aacggcccgg cgacaatccc ctgaatcacg
      841 cggaagaaga tcagcatatt caggctgctg gagacaccac acgcccaaga cgcaatagca
      901 aaggcgatgg tggaccaaag gaacagtttc acttccccga cgcgctttgc cagccagccg
      961 gtaagcggga tcgagatggc attcgccacc ccgaaagaag tgattaccca cgttccttgg
     1021 ctgagcgatg agcccagatt cccggcgata gtggggatcg ccacgttagc aatggtggag
     1081 ttcagcacct gcatgaatgt cgccagtgac agcgcaatcg tcataatgac cagttgcgcg
     1141 ccttccagcg gtttttgctg ttgcatcaca cgcacctctg gattagccag cgttagcttt
     1201 tacgatatcg tcgatcagtt tattgacagg tgccaggctg atttcacgcg cggtacttac
     1261 cgcgaccggt gttgaacgta ctttatttgc cagtacctga ccgtcacggt tagtggtatt
     1321 gacgctcacc agcgtggaca aaccaatgcg cagcgggtat tgctccagct gtttctggtc
     1381 cagttcgata cgcacaggta gacgctgaac gactttgatc cagttaccgg tcgcattttg
     1441 cgccggaagc agtgagaacg cgctacctgt gcccatatcc agaccaacca ctttaccggt
     1501 gtatttcaca tcatcgccgt aaatatcggt ggtgatggtg accggctgac cgatgcgcat
     1561 attggcaatc tgcgtctctt taaagttggc atccacccac atattggtgg ctggaacgac
     1621 cgccatcagc ggcgtcgttg ggctaatttg cgcacccggt tgcacggcac gacgggaaac
     1681 ataaccggtc atcggactga caatacgggt acgctccagc gccagctagg cgttacgtac
     1741 ttcggtggca gcctgttgca cggcaggctg atcttccagt ttagtcccca gaatcatcgc
     1801 ctgattggca ttgtattgtt gaatagcgac gtccagttgc gcctgggcac tggtgacggc
     1861 gtcgcgggcg tgttgcagct cttcgcgacc aatcaggttg gcattgccca gcggcacacg
     1921 gcggttgtag tcgctttgtg ctttcgcgag ggcgattttt tgcacctcaa tattcgcctg
     1981 caactgtttg ctgttaatca tcagctggtg ggtttggcga acgctggaag ccagtgcagt
     2041 tttggctttt tcaaacgcct ggcgagcatc tgtcgggtcg agagtgacca gaacatcgcc
     2101 ttcttttaca aaatcggtgt tatcggccca gactttcgtc acgctgccag acacctgaga
     2161 cataatttga atctgattcc ctgccacgta tgcgtcatcg gtttcttcga aatgacgcag
     2221 taccaaaaac caataaatcc ctatcgctac ggcaataatt ataaagagca aggtgagaag
     2281 gaggagcaga cgcttacgtt tgccgctctt ctttaccggt tgctgcgggg tttgagtctc
     2341 cgcatttgcg ctcatattgt tctccacgat cttcttaact catcggctga gccgacctgc
     2401 ttgccaaaaa ggccagcatg ttgcgatgct ggccggtcat tttttctttt ataaatctgg
     2461 atttttgagc gagatgacgc gttagctcat cgcttcgaga accacaccgt cttgttccat
     2521 ctggtcgaga cgggagagca atttgcgggt gatttgctcg agctgatctt tttctgttgt
     2581 gctgagcgcg gaccagagtt gatgcaggca gttatgctgc ggtggtaaaa cctcgcgcaa
     2641 aaactcgtga cctttttccg ttaattgcag atgcaggcag cggcgatcgt tatcgctttt
     2701 acgacgttcg atccaaccgc gtttttccag ttcatcggca atacgcgtcg cgttggtacg
     2761 ggatgatcca agagcacaac ttaattcaga aggctgaata ctgtggtttt cctgagactc
     2821 cagcgtaatc aacgccataa acaacgtctc gttaatcccc tgagccttca gcattttatt
     2881 gcggttctcc agcagcttgc tttgcatgtg catgcaaaga cgagtcagaa ggatctcctg
     2941 ataaggaaaa tcttcgtggc ggctggcgcg aaattttagc atttgttcaa tgggcgtaaa
     3001 cgaactatcc atttgggtat gacctcatta atttcagccg atatagtaac gacagtgaca
     3061 aataaagtaa atgtattgtt ttagaaaaat gattcttgtg ggttatataa tcgccatcac
     3121 tttccaggcg agcccatagg ccagcgcact aagcagtgtt gggatgataa tgctgcgtgt
     3181 tttatagaaa ctggcaccca gtaccgcgaa gccgaccagc gtgggcacga aacggcgtgt
     3241 atcgtgcatc acttctggtg cggtagagac aaccagcaga gcgcatatcg aggcgatgcc
     3301 aatggtgtcg agcaaaatac ctaccgcgcc acgtttggtt gggcgggcat tacccacacg
     3361 caggcgcagc ggcaaatagc ggaagcaata attcgccgcg ccaaccagta acccaagcag
     3421 cagaacctca tagctcatcg ggcgctcctt gccagaatgc ctggattaac gcagtgaggc
     3481 agccacagac aatgcctgcc agaatggcga cgggaataga aaatagcgtt acgcctgcaa
     3541 gggcaccaac taaagctgcg gtaacgcaaa gagattgttt gcgctggaaa gaggcgagca
     3601 ggaaactcat aaagagtgcc ggaagcataa aacctaatgc agcttcaacg gcgggataac
     3661 cttgcagcaa gccgctgccg gagaatgccc ctattaccgt accaaatacc cacgatgacc
     3721 atgaactgaa ggcaatgccg atcatccagt tctcgctcca gcggcgatta ttgcgtacca
     3781 gttttgcggt ggcggcggca aaaacctcat ccgtcaggcc aaacgcccat agggcggttt
     3841 tcgatttttg cagacgctga ataatacggc tacgcagtga cgggccatac aaaacatggc
     3901 gaacatccat tgccatgacg ctcagtgcag caacccacaa actactcccg gctgccagca
     3961 tcgcggtaat gacgaactgg ctcgcgcctg cataaatgat gcaggagaaa aaaacgcttt
     4021 cgagaggaga gaatcccaga cgggtcgcat tcagaccgaa cgcaaaggcc accggaatat
     4081 aactaataac aatcggtaaa ctgtctttgc atccttccat gaaggtcgcc gaaccaggag
     4141 caggctgtgg agtagggctt tccatagagt gtacgcttaa cgattgttaa tattataaat
     4201 agactgaatg aatatcttaa ccttatcaga ctgatgggct tcttaacacc cttataagtg
     4261 taaagccacg aaaacggttg ctgattgctt aatttgcggc ttcctgacga tgaaaacctc
     4321 gccaccagac cagtaagtta accagggcaa tcgtcgcgcc agccagacaa acgccagccc
     4381 aaccgccatg ttgccaggct gaggctgaaa ttagcgaacc ggcggcaccg ccaataaagt
     4441 agctggtcat gtaacctgcg gtcaggcgat tacgcgcatc aggatgtatt cgataaatta
     4501 ccgtctggtt agtgatatgc acgccctgta cggtgaggtc cagcaccaga atgccgataa
     4561 tcaacgccag tacggaagtg tgaccaaacc agattgccag ccatgaaagt aacagcagaa
     4621 gcagaccgaa agttgtggtg cgatttgccc ttgtcggcaa aaccgcccgc cggacgagcg
     4681 cccaacgctc cggcagctcc cgcaagccca aacagaccaa tgtcaccatc gctgtagtta
     4741 aaaggtggag cggcaagcaa aaaggccatt gaggtccaga gaatgctgaa gttggcaaag
     4801 gtcatgcagc ccagcaacgc gcgggtgcgc aggatcttat cgctgatgaa catgctgaaa
     4861 acggaaccca acaactgtgg gtagttgagg tgggtttctg atttcatttg tggcagacca
     4921 cgccataatg ccagcgccat cagtgccatt aacaccgaag caacccaaaa gacggtgcgc
     4981 cagccgccga ggtttgccag taacccggca acggttcgtg ccagcaatat ccccaacagc
     5041 agaccgctca taatggtgcc aaccactttg ccgcgtttat ccggtgaagc cagcgtcgct
     5101 gccagcggaa ccagaatttg tgccacgact gagaataaac cggttaatgc cgtaccgagg
     5161 atcatcatcg ccagtgactg actgctggca gtgattaaca taccgcccgc tgccagtaag
     5221 gtcatcgaga caatcaggcg gcggcgttca aacatatcac cgaggggaac aagaaacagt
     5281 agacctgcgg catagcccaa ctgcgcggcg gtaacaataa agcctgccga actggcggaa
     5341 agggaaaagt tacgcgcgat ggtgtcgagc aatggctggg cgtaatagtt actggctacc
     5401 gccagaccgg tggcgataga catcagcacg atcagcgccg ggctaagctc atgattaggt
     5461 ttagtcatta tttttgttca tcaggtgatg attaagctgg aaatcaataa tagcggatgt
     5521 tgatgaatat gggatgaaag atagcttgtt gaaatgtgcg atgtggtttg taggcatgat
     5581 aggacgcgtc agcgtcgcat caggcattgt gtacagagcg gcggatgagg cgaaaacgcc
     5641 ttatccgccc gaataaaaat tacttctgcg ctgccagcgc ctcattcacc cagccatcga
     5701 actgctgctg gtgggctttg atccagccat caacgtggcc ctgaatatcg ccttctgagg
     5761 ctttgccgtc atgcataatg gcgttctggg cgttaatatc ggccactggc aactgcataa
     5821 tggcaaacag tttggctgcc gccggatttt tctcggtcca ggctttgttg gcaacgatat
     5881 gcatggtgct gaccgggaag ccgtaattcg cgccattcgg cagtttggta tcggcatttt
     5941 tatcgcccgg cagtgcggag aacggcacct gcaaccagac cacatctttc cctggcttca
     6001 gctcattact cacccagtac ggcgtccagg tgtaataaaa caccggtttg ccctctttgt
     6061 agcgactgat ggtgtcggcc atcatcgctg cgtagttccc ctgattatgc gtcacggtgt
     6121 tggtcagttc atacgcggca agctggtggt tgatcgcacc ttcgcagccc cagccagggt
     6181 tacaaccggt taaatccgct ttaccgtcgc cgttggtatc gaacagtttg gcgatcttcg
     6241 gatctttcag ttgtgcgatg ttggtgattt tgtattggtc ggcggttttc ttatcgatca
     6301 ggtaaccctg tgctgcgccg ttaacaaata ccccttcacg ataaaatttc ttatcgccac
     6361 cggcagcttc gtacatgttg tcatggagtg gcgtccagtt cacggcggtg aaggttgcat
     6421 cgccggaagc aagcgaggtg tagccaacgt tgtaatccac ttcgctgggt ttgttgacgg
     6481 tataacctaa tttctccagc gcacgactga ccagcagcgt ctggaaggtt tcttcagtga
     6541 tggtgctctg aactggatta acagtaatgc ctttgcccgg cagatcggca gcaaaagttt
     6601 gtgtagagat aagcgtggca aacgctgtcg caaaaagtac gctatgtcgc attgttattc
     6661 ctttttttgg cagggtgata atgcccggcg tcaccgggca agtgcagagt tacttaatga
     6721 atgggcgggt cagcagacca acagggccag tggtgtacca gcgacggttg ccgcgactgc
     6781 gtgagtcgcg cccaacggcc tgcgtcaggc gatcgaggat aatggcgagg atcacaatcc
     6841 cgacgccgcc aacggtggca agccccatat ccagacgacc gataccgcga agtaccatct
     6901 gacccagccc gccgacggca atcatcgagg caatgaccac catagaaagg gccagcatca
     6961 gcgtctggtt aacgcccgcc ataatggtcg gcatcgccag cggtaactga actttgaaca
     7021 gcatctggcg cgggctggca ccgaatgagc gcgaggcttc aatcagatcc gccggaacct
     7081 ggttaatccc cagaatggtc agacggataa tcggcggcag cgcaaagatg atcgtcacca
     7141 ccacgcccgg cacgttaccg ataccaaata gcatgacgat tggcaccaga taaacaaacg
     7201 ctggcgtggt ctgcatggca tcaagcagtg gacgaataat tttcgccgct cgcggacttc
     7261 tcgccagcca tatccccaac ggcaaaccga tgacgataca gaacagcagg gcagttaaca
     7321 ccagcgccag agtcaccatt gcctgcgacc aggcaccgat tgcgccgatg gcaatcagcg
     7381 agaccagcgt cgccacgccc attccgaccc cggaaatctg ccaggtgatg agagcgaaaa
     7441 cgataatcgc cactggcgct ggcataccca gcagcaattg ctggaaaccg ttgaggatat
     7501 aatcaaccgg aacgcgcacg ccctggaaga cgggacggaa atgggtaaca acccagtcga
     7561 tcccttcagt gacccaactg tcgagcggga tcagcgtttt atggaacgga tcgagaatat
     7621 taaaatgctc gacgtttggc gcaggcgtac tggtcagcca gtcagcacca ccgccgtcag
     7681 ccggtgcagt cgtcggtgta ccccaggcgt ctgcggattg cgcggcactg tccgccgctg
     7741 gcgtggtatc ccacggatta ttttgatcag ccattattta ccccctcacg atctaaagcg
     7801 cgcagcagca ttcctttcga aatgatgccg acatactgtt ggtcctcgtc gaccacgggc
     7861 accgcacagg gtgcctgtcc gacatgagag agcaactcgc taagaggcgt ttgtgcatcg
     7921 actgctaacg gcgcatcaat cagcgccgca tcaagacctt gctgctgcgt taacgcggtt
     7981 ttaagcgaat cgatggagac tgcgccgaca aacttattac cgcgttcgat aacgtagcca
     8041 tattcgcgat cttcatcctg caataatttc agtgccgaac gtgggccgaa gccaggagtt
     8101 ttacgaatta agccattcgg tgtccggcgg gcaatatctt tcgcactgaa cacctgacta
     8161 atatcaacgc cacggaagaa ggtacggaca taatcattcg ccggattatt gagaatttca
     8221 tccggtgtgc cgacctgtac cacttcacca ttttgcataa tggcaattcg gtcgccaata
     8281 cgcatggctt catcaagatc gtgggaaata aagacaatgg tgcgctgatg tttcgcctgt
     8341 aattttacca gctcatcctg catctcggtg cgaattaatg gatcgagcgc cgagaaggct
     8401 tcgtccatta ataatatatc cggattaatc gctaacgcgc gggctaatcc cacacgttga
     8461 cgcatcccgc cagagagttc atccgggtag ctgtgggcat aattttccag cccgacctga
     8521 cgcagtgcat caagggcttt ttcccggcgt tcttcggcat taattccggc caattccata
     8581 ccgaacgcag tattgtccag cacggtcata tgcggcatta aggcaaagga ctggaagacc
     8641 atcgcaatct tttttctgcg cacctcacgg agttcggcgt cggatatttt ggcaatatcc
     8701 acaccatcaa tcagcacttg cccgcgggtg ggttcaatca ggcgattgag aaggcgtacc
     8761 attgtggatt tacccgagcc ggataatccc atgatgacaa atatctcgcc ttcttcaatg
     8821 gccagactgg cgtcttttac gccaagcgat agcccagttt tttccagaat ttgttctttt
     8881 gaaagtcctt gttcgatata tttgaacgct cgctgtggat gctcaccaaa tattttataa
     8941 agatttttaa tttttaattt aatggccatg caatagaatg atttctttat ttgtttatgt
     9001 cgatatatta cccaatggga atagtcactt ttttctaccc taacatactg agaatctgag
     9061 gcaacccctg agggcaaatg tggcatgaat attgatgcga gcaaatcgcc ggaattttcc
     9121 gtgatataag ggctgagagc aaatcgagaa aagttgatat tttttgtcat agatagcgaa
     9181 aaaacgcctg aatattgtag tttcaggcga ataatgcaac agagtatcag tgtagatcac
     9241 tacagaatat ttgcgtgata aaaagctatt tggcgggaat tatttccctg ctgcgggtag
     9301 tgatattttt gaaaataaca ccctcagaaa ttccagtctt catcttctgt ttcaaccgct
     9361 ttccccatca cataagagga gcctgaaccg gaaaagaaat cgtgattttc atcggcattc
     9421 ggcgaaagcg cggcgaggat tgccggattc acttccgcca tttctgcggg aaataacggt
     9481 tcgtagccca gattcatcaa agccttattg gtgttgtaac agagaaacgc tttcacatcg
     9541 tcagcccacg gggtttcggc gtacagctca tcggtgtatt gcaactcgtt gtcgtagagt
     9601 tccagcaaca aatcgaaggc gaaactcttc aactcttcac gttgtcccag agatatcttt
     9661 tccatgtttt tctgatattt atagcctatg tagtaaccgt ggactgcttc atcgcggata
     9721 atcagacgga tcaggtccgc ggtattggtc agctttccgc ggctggaaaa atacatcggc
     9781 agccagaaac cggaatagaa caaaaaagat tcaagaaaca cactggcgat tttctttttc
     9841 agcggatcat caccgcgata atgttgctga ataatctgag cttttcgctg caacggtgcg
     9901 ttttcttcac tccaggcgta ggcggcatcg acatctttgg tctggcacag cgtcgagaaa
     9961 atcgaactgt aagagcgggc atgaaccgct tccataaagc tgatattcga taataccgcc
    10021 tcttcatgag gcgtgagcgc atcgggcatc agcgaaggcg cgccgataac attttgcagc
    10081 gtgtcgagca gcgtcaggcc agtaaaaacg cgcatcgtca gttgttgttc tacgacagtt
    10141 aatgtctgcc aggcgggaat atcgttcgac agcggcacct tttctggtag ccagaaattg
    10201 ctggtcaggc gattccacac ctccagatct ttatcgtcag atatcttgtt ccagttgatg
    10261 gcgctgatac gtgagagttt catagatatt ccttaaagtg cacaggagac gcagccttca
    10321 atttcagtgc cttccagcgc catctgacgc aggcggatgt aatagagcgt tttgatacct
    10381 ttgcgccagg cgtaaatctg cgctttgttg atatcgcgag tggtggcggt atcggggaaa
    10441 aacagcgtca gcgacagccc ctgatcgaca tggcgagtcg cttccgcgta ggtgtcgatg
    10501 atcttttctg cgccaatttc gtaagcgtcc tgatacagcg ccagattttc gttagtcata
    10561 aacggggcag ggtagtaaac gcgtcctgtt ttgccctctt tgcgtatctc tactttcgcc
    10621 acaatcggat gaatactcga cgtagcatgg ttgatataag agatagaccc ggttggcggc
    10681 accgcctgga gattctggtt gtatatgccg tagcgcatca cgtcgtcgcg cagttgcgcc
    10741 cacatctcac gggtaggtaa cgtaatacag ctgcgggcaa acagttcgcc aactttcgcc
    10801 gttttcggct gccagttacc ttgcagatat tggctgaaat attcaccact ggcatagcgt
    10861 gactgtttga acccggcgaa ggtttcaccg cgttcgcgcg ccagcaacat cgaggtacgc
    10921 agtgcgtgcc aggtgatggt atagaaatag agattggtga aatccagggc ttccggcgaa
    10981 ccataagcga tgccttccct cgccagataa ccatgtaaat tcatctgccc cagtccgatg
    11041 gcgtgcgagg cggcatttcc ggcttcgatg gacggcacgc tgcggatatg actcatatct
    11101 gacaccgccg ttaaaccgcg cacggcagtc tctaccgtgc gggaaaaatc gggggaatcc
    11161 atggtgtgcg caatattcaa cgaacctaaa ttgcaggaaa tatcatggcc tgtgcgggca
    11221 tagtcgagat tctcgtcata ctctgaggcg ctgttaacct gcaaaatttc tgagcagaga
    11281 ttactcatat ttatgcgccc ggcgataggg ttagcacggt ttactgtgtc ttcatacatg
    11341 atgtagggat agccggactc aaactggatt tctgccagtc gctggaagaa atcacgggcg
    11401 ttgaggtatt ttttgcggat gcgttcatcg gcaaccagtt cgtcatagtg ttcgctaatg
    11461 gcgatatcgg caaacggctt gccataaact cgctcaacgt catagggcga aaacagcgcc
    11521 atctgcgcat tctcttttgc cagatggaaa gtgatatccg ggatcaccac gcccagcgac
    11581 agtgttttaa tgcggatttt ttcgtcggca ttttcccgtt tcgtgtcgag aaaacgcaga
    11641 atatcgggat gatgagcatg taaatagact gcacctgccc cctgacgcgc gccgagttgg
    11701 ttggcatagg aaaatgcgtc ttccaacatt ttcatcaccg gaattacgcc agaagattga
    11761 ttttcaatac gtttaattgg cgcgcccgct tcccgcagat tcgacagcaa aaatgctacg
    11821 ccgccgccgc gtttcgacag ctgcagtgcg gaatttaccg cccgaccaat cgactccata
    11881 ttgtcttcaa tacgcagcaa aaaacaggaa accagttcgc cgcgctgctg cttaccgcag
    11941 ttgaggaatg ttggcgtggc tggctgaaag cgtcctgaca gcatttcatc ggtcagttgc
    12001 aacgccagcg tctcatcgcc ttgtgccagc gtcagcgcca ccatcgtgac tcgatcggca
    12061 aaatcttcca gataacgttt accgtcgaat gtcttcaacg tatagctggt gtaaaacttc
    12121 catgccccga ggaatgtctg gaaacgaaaa ccgctggtgt gtgcgtgggc aaacagcgta
    12181 atgacaaagt cgcgagagta gcgattaaga acgctttcat catagtaacc ttcgttgacc
    12241 agccagttca ggcgctgctg ctggctactg aaggtcacac tgttcggacg cacatgcgtc
    12301 gcaataaagg cgtcaacggc ctggcggtct ttatcgaact gaatgcgacc tgcgctatcg
    12361 taaagattaa gcatcgcatt cagcgcgtgg taatccatcg tttcctgcgt caggcattct
    12421 gcggttgtcg ttgccaaaat tcggttactc ctttacgaac gttttcgata tcgctttgcg
    12481 tacccatgag ttcaaaacgg tacagccacg gcacgccgca tttccgggca atcacatctc
    12541 cggcgcggcc atacgcctca ccaaagttgc gattaccaga agcaataacg ccgcgaagca
    12601 acgcccggtt gtgctcgtcg tttaaaaagc gaattacctg tcgtggcacc gcgccagccg
    12661 taccgccgcc gccgtaagag ggcacgatca ggatgtaagg ctcgtctacc tgaatccgtt
    12721 cccgctcatt gagcgggatg cgcaccgcgg gcagacctaa acgttcgata aaacgctgcg
    12781 tgttttcgga gctgctggag aagtagacga gctggctcat gcactggccg cgtgtggcgc
    12841 tggatgcaga cggttaatca tgtccggacg gaaaccagac cagctaagat cgccagcaat
    12901 cactaccggc aactgacgaa agccctgagc acgcaacgct tccgctgctt caggaacgcg
    12961 atcgacatta atcatttcaa aatcaaagcc ccggttttcc atcgcccgtt tggtggcgtg
    13021 gcactgaacg caatcgttac gagtataaat agttatgcgc atgattcgta tttccgttta
    13081 aaatgaagat acggcgcgat gatacgcgtc gggttgtctc tctgttgata cagagatact
    13141 agatgtagtt gaaaaaagat tcaactacac aatatatagc aaatagtgat cgaaattacc
    13201 ctggatatga gcgtgacggg ggagggggat ttttgtgatt caccaggcaa aaagaaaccc
    13261 cgaagacagg cttcggggtc aaagacgcgt atttattatc atttttgcac tacgatttgc
    13321 gcatgcttaa cagtgcgccg ataaaaatac ctaccgcagc tgctgtaccc acgctacacc
    13381 agggtctttc acgaacaaaa gaatccgctc agccaacggc atcgcgcgcg gcttgctgga
    13441 cgcgagtacg accatgcatt cgcgccctgg tttctttcag caacgcctga gctttactgc
    13501 gtgcggcttc agcttcccct ttggcgtcgc tgccccagga cttcaataca gattcgaggc
    13561 tgtcagctaa ttgattgaca tcattctgga tatcctgcac gccatcatca acgtcgttgc
    13621 ggttcggtcg gttaaacata tgatccccca ttagaattcg atgtaatttt cagtgtagac
    13681 cataattttc ttaactgagg ttcatcgcgg ctttatggac ttttctgaaa gcgttgtgaa
    13741 tgcgcaatca ggtaaggtag gcccgcggtt gaaaataacg aggatcaaat atgtatttac
    13801 gaccagacga ggtggcgcgc gtacttgaaa aagtcggttt tactgtcgat gtggtaaccc
    13861 agaaagcgta tggttaccgc cgtggggaaa attatgtcta tgttaatcgc gaagcgcgaa
    13921 tgggacgtac cgctctggta attcatccaa cattaaaaga acgcagttca acactggctg
    13981 aacccgcttc cgatattaaa acttgcgatc attaccagca atttccgctc tatttagcag
    14041 gcgagcgaca cgagcattac ggtatcccgc atggctttag ttcgcgtgtt gcgcttgaac
    14101 gttatttgaa tggtttattt ggcgaagcca gttaaagacg cgactggcga tgccagtcgc
    14161 gaaaagaaga gtcaggcttt tacctgctgg taacggctga ctttaaacag tcggcggcaa
    14221 taatcgagga agtagccata aaccgccccc atcaacatcg aaacaacgat gtttgaactg
    14281 accgccgcca taatctgatg ccagtctgcg cccaccacta acaagatcgc cacatacacc
    14341 ggtgactgga acgtcacata agccaggata tcagccagat tttttatcca gcccgacggg
    14401 ctaactttgc gtgccgcgcg cataaacaga tcgcggtaca taccgtatgg ccatgcaatt
    14461 aagatgttca ccggaatcgc taccaatctg gaataaaaag actgttcgaa gctcattccg
    14521 gagaggaaaa cttcaataca catgttcacg acagaacagt aaacaaccat cgcgaacgta
    14581 tctgcaactg catgacgcaa gcgtgactgc ggtgagaaca tgctaatgct ccttaataaa
    14641 agcgtaatgg gatgttaatg gagatgaaag cagtggatct cattgccatc tgcatttttg
    14701 catagcgtat atcgctgtct atgactaatc aattagtgaa aaatttttat ttagttagtt
    14761 tttgtagata aaatattccg taacggctcg ttttttgtac attgtggatt tttacatgcc
    14821 aatatgattt attttcaatt aaatcaatta gatatgatct tgcctttaat ttcactaatg
    14881 aaagtttggg ggggaggtaa cgctataagc gtagagaatt cggtgtttgt catgaccggg
    14941 tcatttacag catttttgat gccagaaaat gtacattaac agcagaaagc accagtgagg
    15001 ggtgttttct tttattctgt tatattgcat ttttattcac ttcggttttg tcaggcttgc
    15061 ggagttagcg agcagagagc gccactctgt tcaccaataa cctaaattac tcatcaaaat
    15121 ggctaatata taatcttgaa attattctga gagtttcaga aaataacagc tgaaataatc
    15181 tcgcgcagga ctgtaaatag attaaatttt gtggaaatat aataagtgat cgcttacact
    15241 acgcgacgaa atactttttt gttttggcgt taaaaggttt tctttattat gtccgtaatg
    15301 ttacaaagtt taaataacat tcgcaccctc cgtgcgatgg ctcgcgaatt ctccattgac
    15361 gttcttgaag aaatgctcga aaaattcagg gttgtcacta aagaaagacg tgaagaagaa
    15421 gaacagcagc agcgtgaact ggctgagcgt caggaaaaaa ttagcacctg gctggagctg
    15481 atgaaagctg acggaattaa cccggaagag ttattgggta atagctctgc tgctgcacca
    15541 cgtgctggta aaaaacgcca gccgcgtccg gcgaaatata aattcactga tgttaacggt
    15601 gaaactaaaa cctggaccgg tcagggccgt acaccgaagc caattgctca ggcgctggca
    15661 gaaggtaaat ctctcgacga tttcctgatc taattactga ggccggatgt cgctgctagg
    15721 gcgcgtccgg cgtcgcttct caagctcgtt ggttccacgg catcttatct aacaaccttg
    15781 ccattccaca aaaaccgctg attcctgcaa acagtaaccc ggcaccaaca aagccactta
    15841 ataagaagaa accgctattt acggtataac ccagtacaac gccgtttaat attaaaccac
    15901 ccgcagcgat ctgcacctgg cgcattaacg gcaagggttg agatttattt accgctactg
    15961 gcaatccagc tcttttccag ccatcaatcc catcttcgag taaaaatatt tctgcggggg
    16021 cggcaatcgc tgctaattta tcggcgttat tactggtgcg tttacctgcc tggcagtgga
    16081 atataatttg ctcgcgacgt aatttaggtg gaagacctga ctgttccagc acggataatg
    16141 gagccagatc tgcttcagga atatgttcac gaagatattc atcagcgtca cgaatatcga
    16201 ttaactttgc gccgcgtgcg attaattctt gtgcatcatg cggcgaaatg gttgtcaaag
    16261 ccatagtgac tccttacgga caatagacat ttttcagggt ggcgataatg gcatttaccg
    16321 cctcattttt aatggaatat agaatgcgtt gggcatcccg ttggctgttg ataagccctt
    16381 cgtcccgcat acgagcgaga tgctgtgatg tcgcagaggc gctcagtccg gtaatgcgcg
    16441 tcagctctcc cgcgctggtg ccgggggaac cgctaagcat gcacagaatc agcaaccgtt
    16501 tagggtggct cattgctttc aataaggccg ctgcctgttc ggtactggcc tgtaattgcg
    16561 cgagttcagt catatttatt taagtatttt ctaaattaag taaactctaa actaaaaatg
    16621 caacatatac cagcctcagc agcgtaaatg agagtaaaag cgtaagct
//
LOCUS       DADLTY010000089        16388 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.089, whole genome shotgun sequence.
ACCESSION   DADLTY010000089 DADLTY010000000
VERSION     DADLTY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.089"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="JN334_002462"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="JN334_002462"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAY7438570.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="JN334_002463"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="JN334_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HAY7438571.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2339..2554
                     /locus_tag="JN334_002464"
     CDS             2339..2554
                     /locus_tag="JN334_002464"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438572.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="JN334_002465"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="JN334_002465"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAY7438573.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /locus_tag="JN334_002466"
     CDS             4449..5843
                     /locus_tag="JN334_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY7438574.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="JN334_002467"
     CDS             5920..6417
                     /locus_tag="JN334_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAY7438575.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="JN334_002468"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="JN334_002468"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HAY7438576.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="JN334_002469"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="JN334_002469"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAY7438577.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="JN334_002470"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="JN334_002470"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAY7438578.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9030..9665
                     /locus_tag="JN334_002471"
     CDS             9030..9665
                     /locus_tag="JN334_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAY7438579.1"
                     /translation="MTAPFFPRILGAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKITPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="JN334_002472"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="JN334_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAY7438580.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="JN334_002473"
     CDS             complement(10257..11237)
                     /locus_tag="JN334_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAY7438581.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="JN334_002474"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="JN334_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HAY7438582.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="JN334_002475"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="JN334_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HAY7438583.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /locus_tag="JN334_002476"
     CDS             12540..12830
                     /locus_tag="JN334_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HAY7438584.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="JN334_002477"
     CDS             12838..13431
                     /locus_tag="JN334_002477"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HAY7438585.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="JN334_002478"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="JN334_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAY7438586.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14874..15837
                     /locus_tag="JN334_002479"
                     /pseudo
     CDS             14874..15837
                     /locus_tag="JN334_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15882..16385
                     /locus_tag="JN334_002480"
     CDS             15882..16385
                     /locus_tag="JN334_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAY7438587.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4058 a         4220 c         4124 g         3986 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcccttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atcgccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccaccttc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgct
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgtgct
    12061 cattgtgatt tttacacccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact
    14641 gaataaatat cgacgacatt tattatatat atttagtgaa tatttactaa tcattcattg
    14701 attataaata tgtacaacct ggcgcacaaa tttaatattt aatttctgac tttatttcaa
    14761 gttgtgataa acatagacaa gttgtcagac aggttaacca atttgtcgca caaatcactg
    14821 ataagcacaa ttagatatca accaatttcc atccttcaac agggagtttt tttatgaaaa
    14881 tgaggtcatt tacccgctca ttagtctgcg catccttgct ggctctggtt agcactggcg
    14941 taaacgccgc agaaaaagtg gcgttaaagt tagcacacaa cctggaaaga agtcatgttg
    15001 tacatcagag ttttgaagag ctggcgaaag aggtaaaaca gctgtcaaaa ggtaatatgg
    15061 tgatccgtat ttatcccagc agccagatgg gtaatgcgcg agaaactatg gaattgttgc
    15121 aaaatggcgc actggatatg accaaaggtt ccgccagtga tctggagtct tttgataata
    15181 tttatgctat ctataatttg ccgttcttat taagttgttt ttggtgaagt tggtaaagaa
    15241 attatggact caacaaaaga caaaggcttt tttgctttat cagcttatgt agcgggtact
    15301 cgcagtttct atgcgaaaaa accgattaca aagccagaag atcttaaagg actgaaaatt
    15361 cgtgttcagc caagtccgac caccatcaaa atgattgaac tgatgggtgg ttcacccact
    15421 ccaatttcat ttggcgaagt ttatactgcg atgcagcaag gtgtagtaga tggcgcagaa
    15481 aataacgtgc cttcctggat gcagactcga catattgaaa ttgccaaagt tttttctgaa
    15541 gatgaacacg cctcaattcc tgactttttg gttatctcca ccaaaacatg gaataaatta
    15601 accccagaac agcaacaaat cctcgaaaca gcggcgaaga aatccgaagc ttatcaacaa
    15661 aaattgtggg aaaaaattga cgctgacaca cgtgctcagg cgaaagctat ggggggtgaa
    15721 attgtaaaag tggataaagc gccattccgt aaagccgtgc aacctctgtt tgacgacttc
    15781 aaaaaagatc caaaacaagc tgctttgtta gagaaattcg acaacgctgc tcaataatat
    15841 tccgggccag cctctggccc tgattttcca ggatgcttac aatgatattt aaccgcctga
    15901 aacttgcggt agaccgcgtc attgccacgt tttctgtcgc cgtaatgctt gctctggttg
    15961 tatgcgtagt ctggcaagta tttagccgct acgtacttaa ccagccaagt acattaaccg
    16021 atgagctggc acgctttttg atgatctggg taggattatt aggtgctgca tataccgtcg
    16081 gcgcgcagcg acatctctct attgatttat ttgctctcgc cctgaataag cgcaaacaat
    16141 tacttcttag tatagtcatt aacgttctca ttttaggttt tgccggttcg gtcattgtga
    16201 ctggtggcct gaaattaatt gataaaacac tggcaacatc acaggtatct gccgctatgc
    16261 aaataccgat gggatacgta tatatcattc tgccattaag tggagtggta atgatgtttt
    16321 atgccttgtg ttttattaat caaagcattc aacaactgaa acaacctgcg caggaggcca
    16381 gttaatgg
//
LOCUS       DADLTY010000090        16236 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.090, whole genome shotgun sequence.
ACCESSION   DADLTY010000090 DADLTY010000000
VERSION     DADLTY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.090"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..1100
                     /locus_tag="JN334_002481"
     CDS             <1..1100
                     /locus_tag="JN334_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HAY7438588.1"
                     /translation="KVVGLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIK
                     GVGEGPIEAIIEARNKGGYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAA
                     LMNSLGDALKAADQHAKAEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERE
                     TLGLYLTGHPINQYLKEIERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNR
                     IGICTLDDRSGRLEVMLFTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVM
                     DIDEAREKYARGLAISLTDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLR
                     FGATWRVSPSDRLLNDLRGLIGSEQVELEFD"
     gene            1113..2072
                     /gene="accA"
                     /locus_tag="JN334_002482"
     CDS             1113..2072
                     /gene="accA"
                     /locus_tag="JN334_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAY7438589.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            2171..4312
                     /gene="ldcC"
                     /locus_tag="JN334_002483"
     CDS             2171..4312
                     /gene="ldcC"
                     /locus_tag="JN334_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HAY7438590.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            4369..4758
                     /locus_tag="JN334_002484"
     CDS             4369..4758
                     /locus_tag="JN334_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY7438591.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            4823..6121
                     /gene="tilS"
                     /locus_tag="JN334_002485"
     CDS             4823..6121
                     /gene="tilS"
                     /locus_tag="JN334_002485"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAY7438592.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(6170..6430)
                     /gene="rof"
                     /locus_tag="JN334_002486"
     CDS             complement(6170..6430)
                     /gene="rof"
                     /locus_tag="JN334_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HAY7438593.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(6417..6617)
                     /locus_tag="JN334_002487"
     CDS             complement(6417..6617)
                     /locus_tag="JN334_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HAY7438594.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            6783..7328
                     /locus_tag="JN334_002488"
     CDS             6783..7328
                     /locus_tag="JN334_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HAY7438595.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            7325..7747
                     /gene="arfB"
                     /locus_tag="JN334_002489"
     CDS             7325..7747
                     /gene="arfB"
                     /locus_tag="JN334_002489"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY7438596.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            7761..8471
                     /gene="nlpE"
                     /locus_tag="JN334_002490"
                     /gene_synonym="cutF"
     CDS             7761..8471
                     /gene="nlpE"
                     /locus_tag="JN334_002490"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HAY7438597.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(8626..9450)
                     /locus_tag="JN334_002491"
     CDS             complement(8626..9450)
                     /locus_tag="JN334_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HAY7438598.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(9503..11221)
                     /gene="proS"
                     /locus_tag="JN334_002492"
     CDS             complement(9503..11221)
                     /gene="proS"
                     /locus_tag="JN334_002492"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAY7438599.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(11332..12039)
                     /gene="tsaA"
                     /locus_tag="JN334_002493"
     CDS             complement(11332..12039)
                     /gene="tsaA"
                     /locus_tag="JN334_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAY7438600.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(12036..12440)
                     /gene="rcsF"
                     /locus_tag="JN334_002494"
     CDS             complement(12036..12440)
                     /gene="rcsF"
                     /locus_tag="JN334_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HAY7438601.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(12558..13373)
                     /gene="metQ"
                     /locus_tag="JN334_002495"
     CDS             complement(12558..13373)
                     /gene="metQ"
                     /locus_tag="JN334_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HAY7438602.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(13413..14066)
                     /gene="metI"
                     /locus_tag="JN334_002496"
     CDS             complement(13413..14066)
                     /gene="metI"
                     /locus_tag="JN334_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HAY7438603.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(14059..15090)
                     /gene="metN"
                     /locus_tag="JN334_002497"
     CDS             complement(14059..15090)
                     /gene="metN"
                     /locus_tag="JN334_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HAY7438604.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            15278..15853
                     /gene="gmhB"
                     /locus_tag="JN334_002498"
     CDS             15278..15853
                     /gene="gmhB"
                     /locus_tag="JN334_002498"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAY7438605.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3970 a         4065 c         4335 g         3866 t
ORIGIN      
        1 agaaggtggt gggcctggtg gatgagtgct ggcggatggg gctgaaaatc ctgccaccag
       61 atataaactc cggtctttac catttccacg tcaacgacga tggcgaaatc gtgtatggta
      121 ttggcgccat caaaggggta ggtgaaggtc cgattgaggc catcatcgaa gcccgtaata
      181 aaggcggcta cttccgcgaa ctgtttgatc tctgcgcccg taccgacacc aaaaagttaa
      241 accggcgggt gctggaaaaa ctgatcatgt ccggggcgtt tgaccgtctt gggccacacc
      301 gcgcggcgct gatgaactcg ctgggtgatg cgttaaaagc ggcagatcaa cacgcgaaag
      361 cggaagctat cggtcaggcc gatatgttcg gcgtgctggc cgaagagccg gaacaaattg
      421 aacaatccta cgccagctgc caaccgtggc cggagcaggt ggtattagat ggggaacgtg
      481 aaacgttagg cctgtacctg accggacacc ctatcaacca gtatttaaaa gagattgagc
      541 gttatgtcgg aggcgtaagg ctgaaagaca tgcacccgac agaacgtggt aaagtcatca
      601 cggctgcggg gctcgttgtt gccgcgcggg ttatggtcac caagcgcggc aatcgtatcg
      661 gtatctgcac gctggatgac cgttccgggc ggctggaagt gatgttgttt actgacgccc
      721 tggataaata ccagcaattg ctggaaaaag accgcatact tatcgtcagc ggacaggtca
      781 gctttgatga cttcagcggt gggcttaaaa tgaccgctcg cgaagtgatg gatattgacg
      841 aagcccggga aaaatatgct cgcgggcttg ctatctcgct gacggacagg caaattgatg
      901 accagctttt aaaccgactc cgtcagtctc tggaacccca ccgctctggg acaattccag
      961 tacatctcta ctatcagagg gcggatgcac gcgcgcggtt gcgttttggc gcgacgtggc
     1021 gtgtctctcc gagcgatcgt ttattaaacg atctccgtgg cctcattggt tcggagcagg
     1081 tggaactgga gtttgactaa tacaggaata ctatgagtct gaatttcctt gattttgaac
     1141 agccgattgc agagctggaa gcgaaaatcg attctctgac tgcggttagc cgtcaggatg
     1201 agaaactgga tattaacatc gatgaagaag tgcatcgtct gcgtgaaaaa agcgtagaac
     1261 tgacgcgtaa aatcttcgcc gatctcggtg catggcagat tgcgcaactg gcacgccatc
     1321 cgcagcgtcc ttataccctg gattacgttc gcctggcatt tgatgaattt gacgaactgg
     1381 ctggcgaccg tgcgtatgca gacgataaag ctatcgtcgg tggtatcgcc cgtctcgatg
     1441 gtcgtccggt gatgatcatt ggtcatcaaa aaggtcgtga aaccaaagaa aaaattcgcc
     1501 gtaactttgg tatgccagcg ccagaaggtt accgcaaagc actgcgtctg atgcaaatgg
     1561 ctgaacgctt taagatgcct atcatcacct ttatcgacac cccgggggct tatcctggcg
     1621 tgggcgcaga agagcgtggt cagtctgaag ccattgcacg caacctgcgt gaaatgtctc
     1681 gccttggcgt accggtagtt tgtacggtta tcggtgaagg tggttctggc ggtgcgctgg
     1741 cgattggcgt gggcgataaa gtgaatatgc tgcaatacag cacctattcc gttatctcgc
     1801 cggaaggttg tgcgtccatt ctgtggaaga gcgccgacaa agcgccgctg gcggctgaag
     1861 cgatgggtat catcgctccg cgtctgaaag agctgaaact gatcgactcc atcatcccgg
     1921 aaccgctggg cggcgctcac cgtaacccgg aagcgatggc ggcatcgttg aaagcgcaac
     1981 tgctggcgga tctggccgat ctcgatgtgt taagcactga agatttaaaa aatcgtcgtt
     2041 atcagcgcct gatgagctac ggttacgcgt aattcgcaaa agttctgaaa aagggtcact
     2101 tcggtggccc ttttttatcg ccacggtttg agcaggctat gattaaggaa ggattttcca
     2161 ggaggaacac atgaacatca ttgccattat gggaccgcat ggcgtctttt ataaagatga
     2221 gcccatcaaa gaactggagt cggcgctggt ggcgcaaggc tttcagatta tctggccaca
     2281 aaacagcgtt gatttgctga aatttatcga acataaccct cgaatttgcg gcgtgatttt
     2341 tgactgggat gagtacagtc tcgatttatg cagcgatatc aatcaactta atgaatatct
     2401 cccgctttat gccttcatca acacccactc gacgatggat gtcagcgtgc aggatatgcg
     2461 gatggcgctc tggttttttg aatatgcgct ggggcaggcg gaagatatcg ccattcgtat
     2521 gcgtcagtac accaacgaat atcttgataa cattacgccg ccgttcacga aagccttgtt
     2581 tacctacgtc aaagagcgga agtacacctt ttgtacgccg gggcatatgg gcggcaccgc
     2641 atatcaaaaa agcccggttg gctgtctgtt ttatgatttt ttcggcggga atacccttaa
     2701 ggctgatgtc tctatttcgg tcaccgagct tggttcgttg cttgaccaca ccgggccaca
     2761 cctggaagcg gaagagtaca tcgcgcggac ttttggcgcg gaacagagtt atatcgttac
     2821 caacggaata tcgacgtcga acaaaattgt gggtatgtac gccgcgccat ccggcagtac
     2881 gctgttgatc gaccgcaact gccataaatc actggcgcat ctgttgatga tgaacgatgt
     2941 agtgccagtc tggctgaaac cgacgcgtaa tgcgttgggg attctggggg ggatcccgcg
     3001 ccgtgaattt actcgcgaca gcatcgaaga gaaagtcgct gccactacgc aagcacaatg
     3061 gccggttcat gcggtgatca ccaactccac ctatgatggt ttgctctaca acaccgactg
     3121 gatcaaacag acgctggatg tcccgtcgat ccacttcgat tccgcctggg tgccgtatac
     3181 tcattttcat ccaatctacc agggtaaaag tggtatgagc ggcgagcgtg ttgcgggaaa
     3241 agttttcttc gaaacgcaat cgacccacaa aatgctggcg gcgttatcgc aggcttcact
     3301 gatccacatt aaaggcgagt atgacgaaga ggcgtttaac gaagccttta tgatgcatac
     3361 caccacctcg cccagttatc ccattgttgc ttcggttaag acggcggcgg cgatgctgcg
     3421 tggtaatccg ggcaaacggc tgattaaccg ttcggtggag cgggcgctgc attttcgcaa
     3481 agaggtccag cggctgcggg aagagtctga cggttggttt ttcgatatct ggcaaccgcc
     3541 gcaggtggat gaagccgaat gcaggcccgt tgcgcctggc gaacaatggc acggctttaa
     3601 cgatgcggat gccgatcata tgtttctcga tccggttaaa gtcactattt tgacaccggg
     3661 gatggacgaa cagggcaata tgagtgagga ggggatcccg gcggcgctgg tagcaaaatt
     3721 cctcgacgaa cgtgggatcg tagtagagaa aaccggccct tataacctgc tgtttctctt
     3781 tagtattggc atcgataaaa ccaaagcaat gggattattg cgtgggttga cggaatttaa
     3841 gcgctcttac gatctcaacc tgcggatcaa aaatatgctg cccgatctct atgctgaaga
     3901 tcccgatttc taccgcaata tgcgtattca ggatctggcg caagggatcc ataagctgat
     3961 tcgtaaacac gatctttccg gtttgatgtt gcgggcattc gatactttgc cggaaatgat
     4021 catgacgcca catcaggcat ggcagagaca gattaaaggt gaagtagaaa ccattgcgct
     4081 ggaacaactg gtcggtagag tatcggcaaa tatgatcctg ccttatccac cgggcgtacc
     4141 gctgttgatg ccaggagaaa tgctgaccaa agagagccgc acggtactcg attttctact
     4201 gatgctttgt tccgtagggc aacattaccc cggttttgaa acggatattc acggcgcgaa
     4261 acaggacgaa gacggcgttt accgcgtacg agtcctaaaa atggcgggat aacttgccag
     4321 agcggcttcc gggcgagtaa cgtgctgtta acaaataaag gagacgttat gctgggttta
     4381 aaacaggttc accatattgc gattattgcg acggattatg cggtgagcaa agctttctac
     4441 tgcgatattc ttggtttcac gctgcaaagc gaagtctatc gcgaagcgcg cgactcgtgg
     4501 aaaggggatt tggcgcttaa tgggcaatat gtgattgagc ttttctcttt tccgttcccg
     4561 ccggagcgcc ccagccgacc ggaagcttgc ggcctgcgtc atctggcttt tagcgttgat
     4621 gacatcgatg cggcagtggc gcaccttgaa agccataacg tgaagtgtga agccatccgt
     4681 gtcgatccat acacgcaaaa acgcttcacc ttctttaacg atccggacgg gctgccgttg
     4741 gaactgtatg agcagtaagg cttgtcatcg ccgcatttgc ccggtaacgt gccgggcatt
     4801 gctactgtaa aatcgcacca tcatgacact cacgctcaat agacaacttc tcacctcacg
     4861 ccagattctg gtggccttta gcggcgggct tgactccacc gttctgctgc atcagttggt
     4921 gcagtggcgg acggaaaatc cgggcgtcac tctgcgcgct attcatgtgc atcacggttt
     4981 aagtgccaat gccgatgcct gggttacgca ttgcgaaaac gtctgccaac agtggcaggt
     5041 gccgctggtg gtcgaacgcg tacaacttgc gcaagagggg ctggggattg aggcccaggc
     5101 gcggcaggca cgttatcagg catttgcccg taccctattg cccggcgaag tgctggtcac
     5161 cgcgcaacat ctcgacgatc aatgtgaaac ctttctgctg gcgctaaaac gcggtagcgg
     5221 ccctgccggg ctttcggcta tggcggaagt ttcggagttt gctggaacac ggcttatccg
     5281 cccgttgctt gcccgcacgc ggggggaact ggagcagtgg gcattggcgc atggtttacg
     5341 ctggattgaa gacgaaagta atcaggacga ctcatacgat cgtaactttc tgcgcctgcg
     5401 cgtagtgccg ttattgcagc agcgttggcc gcattttgcg gaagcaacgg cccgcagcgc
     5461 cacactttgt gctgaacaag agagtctgtt ggatgaactg ctggcggatg atttagcaca
     5521 ctgccaaacg tcgcagggga cgctgcagat tgcgccaatg ctggcgatga gtgatgcccg
     5581 tcgcgcggcg attattcgcc gctggctggc agggcagaat gcaccgatgc cttcccgcga
     5641 cgcgctggtg cgcatctggc aggaagtggc gctggcgcgg gaagatgctt ccccctgttt
     5701 acgtttgggc gcctttgaaa tccgccgcta tcagtcgcaa ctgtggtgga ttaaatccgt
     5761 caccgggcaa agcgaaacca ttgtgctgtg gcagacgtgg ctacaaccgc tggaattacc
     5821 ggcggggctg ggaaccgtac agcttactgc gggaggcgat attcgccctc cgcgtgcaga
     5881 cgaagcggtc agcgtgcgtt tcaaagcgcc aggattgctg catattgtcg ggcgcaacgg
     5941 cggacgtaag ctgaagaaaa tctggcaaga gttaggcgtg ccgccgtggc tacgtgacac
     6001 cacgccactg ctgttttatg gagaaacgct gattgcggcg gcaggggtat ttgtgacgca
     6061 agaaggtgtg gctgaaggtg agaatggcgt aagttttgtc tggcagaaaa cgcttagtta
     6121 agtgaaagcc ggataagacg catcaaacgt cgcatccggc gaaagtcaat caggattcgc
     6181 ttaccaccac cgtaccgatt tcagggtggc taaagctggt aattttatcc agacgcagct
     6241 cgcgggtttc gccagcggcc tcgacgacca gatactccac atttttgcgg gagactaaat
     6301 cactggcttt tgcctgcaat ttttcgccat ctttcagctc aagtgtcagc attaaatgat
     6361 gctggcaggc gagctcaaga ttatcgtaat catcacaatt aattggttga tacgtatcat
     6421 tcattgacat aatcgctcac cagtaagttt gccgcagcgt atgctgcttt ttccctgaca
     6481 gcctcagaaa gggcgtcgtc ggcagccatt tcattcagca ctttcaaaac gcagcccagc
     6541 gcgtccggaa cgtatcctaa gtctccactg gcgatttccg cgtaccgctt gcgtattaac
     6601 tcacaatatt tttccacatg ccctcctgtc agcactctga cttaaccgtg gatgcaagtc
     6661 taagcctacg aagataaact ctgtttcgca aggtgactat accacactca tttctgcaat
     6721 atcagcgccg caactgcacg tattccgtta caatggcctc ctgattcgaa aggagttttc
     6781 ttatggcgct taaagcgaca atttataaag cgacggttaa tgtggccgat ctcgaccgca
     6841 accagtttct cgatgcctct ctgacgctgg cgcgccaccc ttcagaaacc caggagcgta
     6901 tgatgctgcg cttgctggcg tggctgaaat atgctgatga acgtctgcaa tttacccgtg
     6961 gtttgtgtgc cgatgatgag ccggaagcgt ggctgcgtaa cgatcacctg ggcattgatt
     7021 tgtggattga gctggggctg ccggatgagc gacgaattaa gaaagcctgc acccaggccg
     7081 cagaagtggc gctgtttacc tataatagtc gggcggcgca aatctggtgg cagcaaaatc
     7141 agagcaaatg tgtgcagttt gccaatcttt ccgtctggta tctggacgat gaacaactgg
     7201 cgaaagtaag cgcctttgcc gatcgtacca tgacgctgca ggcaacgatt caggatggtg
     7261 ttatctggtt atcggatgat aagaataatc tggaagttaa cttaaccgcc tggcaacaac
     7321 cttcatgatt gtgatttccc gacatgttgc tattcccgat ggtgagcttg agatcaccgc
     7381 cattcgtgcg cagggcgctg gcgggcagca tgttaataag acctcaacgg ctattcatct
     7441 gcgttttgac attcgggcgt ccagcctgcc agagtattac aaagagcgtc tgctcgccgc
     7501 cagccatcat ttgatcagca gtgatggcgt gattgtcatt aaggcacagg aataccgcag
     7561 tcaggaactg aaccgcgaag ccgctctggc ccggctggtg gcagtgatta aagatttaac
     7621 aaaagaacaa aaagcccgac gacccacgcg gcccacccgt gcatcgaaag agcgcaggct
     7681 ggcatcgaaa gcacaaaaat caagcgtgaa ggcgatgcgc ggcaaagtac gcagcggtcg
     7741 ggaataaaaa gaaggaatgg atggtgaaaa aagcgatagt gacagcgatg gctgtaatca
     7801 gcctctttac tctgatggga tgtaataatc gggccgaagt cgatacgctt tctccggcgc
     7861 aggctgccga actgaaaccg atgccgcaaa gttggcgcgg cgtgctaccg tgtgccgatt
     7921 gcgaaggaat cgaaatctct ctgttcctcg aaaaagacgg aacatgggtg atgaatgagc
     7981 gttatctcgg tgctcgtgaa gaaccttcct ccttcgcttc ctacggtaca tgggcgcgaa
     8041 ccgctgacaa gctggtatta accgacagca aaggtgaaaa gtcatattat cgggcgaaag
     8101 gcgatgcgct ggagatgctc gatcgtgaag gcaatccaat tgaatcgcag ttcaactata
     8161 cgctggaacc ggcacaatcc agtttaccta tgacgccgat gaccctgcga ggcatgtatt
     8221 tttatatggc tgatgcggcg accttcactg attgcgcgac cggaaaacgt ttcatggtag
     8281 cgaataacgc agagctggag cgtagctacc tggctgcgcg cggtcacagt gaaaaaccgg
     8341 tgttactgtc agtagaaggt cactttacgc ttgaggctaa tccggatact ggtgcgccaa
     8401 ccaaagtatt agcgcccgat acggcgggta aattttaccc gaataaggat tgcagtagtt
     8461 tggggcagta aacctgctta aggcagaaac aaacgcaaaa ttgcctgatg cgctgcgctt
     8521 atcaggccta cagcatataa tgcaacattt tgaattagca cgattttgta ggccggataa
     8581 ggcgtttacg ccgcatccgg cattttgtgc acgaggctgg ctttatcact gagtcagccc
     8641 aacaaagcga cccgccttga tataaatccc aggcttcagg tgcccaacat actgtaactg
     8701 cgtttctggc ttaaacgcag agacatcacc aatacgcata tgcatcagat cggcagggct
     8761 aatccagccc gattgcgcca gtgtaagtgg atgcccggat ttggcaaatg catcagtgac
     8821 aaattccgaa caaaaccacg actttttgtc tccttcgcac acactgctta actgcgcttt
     8881 cgccaggtcg ctgacgcact gttggcgaaa atcctcggag aacggattca gtgagcacat
     8941 ctggcgagtc accataaagg gaataaattc gacaatgccg cgatagttat aaccgctatc
     9001 tttgatttta ttggcaaacg cggtgatatc tgtggcttgt tgcggggtaa gatccgggac
     9061 tcgtaagacg aaaagcttat cactatgctt catcgctttt ttaagggaaa caatctggac
     9121 gccagcgcct gtcgcttctg caacgttatt atcacccaga aagattgcaa cgtgactcac
     9181 agaggaagtg ctgaagacgc ggattccaaa tgaggttacc ccaaggcttg aggagaacag
     9241 caaatcgccg ggtttgagat caggtgccgt tatttctttt attgattgtt cggtgaaaga
     9301 gctttgatgc tggaatttga cagcccatgt ttttgcctcg gcatctactg ctgtggctga
     9361 tgggtctggc tggctgatat caacggtaca agccgaaagt aaaagaaagc tggggagaag
     9421 caggcggcag tacgcctttg gtttatccat tttatacaat ccatgtaaaa aagggccctg
     9481 aaattcagga ccctttctgg catcagcctt taatctgttt caccagatat tcgacgatgt
     9541 caccagtctt aattaactgt ttctcgccgt tgcgacgata tttatattcg atatcgtcgt
     9601 tgtcgaggtt acggtcgccc agcacaatag tgtgcggaat accgatcagt tccatatcag
     9661 caaacatcac gcccggacgc tctttgcggt catccagcag cacttcgata ccttgtgcac
     9721 gcagttcgct gtacagtttc tcagcaagct cttgtacgcg gaaggatttg tgcatgttca
     9781 tcggcagaat cgccacctgg aacggcgcga tagcgtcagg ccatacgatg ccgcgttcgt
     9841 cgtagttctg ctcaatcgcc gcagctacca cacgcgttac cccgataccg tagcaaccca
     9901 tcgtcaggat ttggttacgg ccatcttcac cctgtacgga ggctttcagt gcttcggagt
     9961 acttggtacc cagctggaag atgtgaccaa cttcgatacc acgtttgatc agcagcgtac
    10021 cctggccatc cgggcttgga tcgccagcca ccacgttacg gatatctgca acttccgggg
    10081 tagcgacatc gcgatcccag ttgataccga agtagtgttt accatcgatg ttagcaccag
    10141 cagcgaaatc actcatcgcc gcaacggtac ggtcaatcac caccggaatc ggcatgttta
    10201 ccggacccag tgaacccgga ccggctttaa ccacagcacg aatttcttct tcggtcgcga
    10261 aagtcagcgg gcttgcaacc tgcggcagtt tttctgcttt aacttcgttc agctcgtgat
    10321 caccgcgcac cagcagcgca accagcggga agctactgcc ttcaaccgct ttaaccagca
    10381 gagtcttaac cgttttctca atcggcagat tgaactgttc aaccagttcc gcgatggttt
    10441 tcgcgttcgg cgtatcaacc agcgtcattt cctgggtagc agcagcgcgc ggttctttcg
    10501 gcgcgatagc ttctgccagt tcaatgttcg ctgcatagtc agaggtgtcg gagaagacca
    10561 catcgtcttc accgctctgc gccagcacct ggaattcgtg agaggcgctg ccgccgatag
    10621 aaccggtgtc ggcctgtacg gcgcggaaat ccagccccat gcggctgaag attttgctgt
    10681 aggccgcata cattgcatcg taggtttcct gcagggattc ctgagaagta tggaaagagt
    10741 aagcatcttt catcaggaat tcgcgggaac gcatgacgcc gaaacgcgga cgcacttcgt
    10801 cgcggaactt ggtctggatc tgatagaagt tcagcggcag ctgtttgtaa gagctaagct
    10861 cgttacgaat caggtcagtg ataacttctt catgagttgg gccgagtacg aacggacgct
    10921 cgccacggtc aacaaaacgc agcagttccg gaccgtactg ttcccaacga ccactctctt
    10981 gccacaaatc ggctggctga accaccggca tcgacacctc gatcgcaccg gcgttgttca
    11041 tctcttcacg cacgatgttt tcgacttttt tcagaacgcg cacgccggtc ggcagccagg
    11101 tatataaccc ggaggccagc ttgcggatca tcccggcgcg cagcatcagc tgatggctga
    11161 tcacctcggc gtcggcaggt gtctccttga gagtggagag caggtattgg ctagtacgca
    11221 tgttgttacg gttccagttg gaaggtagaa caggctcaag gcgagcctgg gacaaaaaaa
    11281 gtgatttagt ttaccagtgc aaaagaaatg tcaaaagaga agggcgtgaa tttaacgcgg
    11341 ttccagcgca aagacttcaa aacctgcgtc ggtgacgcgc cagcgaacgt taaaatcatg
    11401 tagccagacg gcataggttt tgcccgtttc ctcaccttta cgataggccg ggcgcgggtc
    11461 ctgcgccagt acttcgcgga taaacagcgt taactgcgga taacgcttct ccagcgtcaa
    11521 aagctgcttt tcgacctctg cggtaaaact caccgccatc tctgcagctg gcgcactttg
    11581 cgcatagctg gcactggcat cgggaagcga ttcggcaaag gggagatacg gtttgatatc
    11641 cactaccggc gtaccatcga ccagatccag actgccgagc ttcagaatca cgctgccttt
    11701 atggcaaaca acctctttca gctctaccag cgacatgcca attgggttag ggcggaaagt
    11761 agagcgtgtt gcgaaaaccc ccattctggc gttaccgccg aggcgcggtg gacgcacagt
    11821 cggacgccag ccgccttcca tcgtttgatg aaagacgaaa aggatccata aatggctgaa
    11881 tgcttccagg ccgcgaacgg cgtcggcctg gttgtaggaa gctatgagat gcagttcgcc
    11941 gttggcgctt tttaccagac ctggctggcg cggaacggcg aatttttctt tatagggcga
    12001 gcgaataacg cctatttgct cgaactggaa actgctcatt tcgccgtaat gttaagcgca
    12061 gagccgatac atacagcctg acgatagcag cccggcgtac cgctggtgac ttcgcagcta
    12121 tgcagtaata cagcattggc tttcatttta gaggcattga tttgcatctg cttacgtgcg
    12181 gttggaatgc tcggcggaga gtcctgatta gaggcctggc aagagtcgcc actgacttca
    12241 ccgagatcgc ggaacggttt gccgactaat tcttctgcat tggtataaat tcggaccggc
    12301 gtggcgcgcg gcgctttcgg ttttgcaggc tccgctttcg gctggggtgc agtgctttga
    12361 acgggttcga caggggatct gcttaacatg gaacagccgc ttagcatgag tgctactaaa
    12421 cagatcggta aagcacgcat agtatttcct caatgtatga tcaaaacgtc aatattgaat
    12481 caggagcttg taaaaatgac aagacgggca agcgcccgtc ctgaatgata ttacaaattg
    12541 tggaaacagc ctaaaaatta ccagccttta acagcgccgc cgttaaacac tttgtttgct
    12601 gcttcgtaaa cttcatcaga ctgataagcc tggacgaatt tcttcacgtt ctcggcgtct
    12661 ttgttatctt cacgcgttac gatcaggttt acgtatgggg agtctttatc ttcgacaaag
    12721 ataccgtctt tcgccggagt caggccaatc tggctggcat aggtggtatt gataactgcc
    12781 agagcgattt gcgcgtcgtc cagagaacgc ggcagttgcg gtgcttccag ttcaacaatt
    12841 ttcagatttt ttgggttctc aacaacatca agaacggtcg gcagcaggcc aacgccatct
    12901 ttcagtttga tcaagcccac tttttgcagc agcagcagtg aacgaccaag attagttgga
    12961 tcgtttggca cggcaacctg cgaaccatcc tgcagttcat ccagtgattt gattttcttg
    13021 gagtaaccag caatcggata aacaaaagtg ttgcctactg cgaccagttt gtagccacga
    13081 tctttcagtt gctgatcaag gtacggttta tgctggaagg cgttggcgtc gatatcgcct
    13141 ttgctcaatg cttcgtttgg cagaacatag tcgttgaagg ttaccagctc aacgtccagg
    13201 ccatatttgt ctttcgcaac tttctgcgcg acttctgcta cctgctgttc ggcaccaaca
    13261 atcacgccga ctttaatgtg gtttggatct ttttcatcct gaccgcagcc taccagtgcc
    13321 agtgatccaa tcagggctcc cactgccgca aaggttttga atttgaacgc cataccttat
    13381 tccttcttca attatttatg ttgtgttgaa cgttacttgc gagtgacagc ccggacgatg
    13441 cggtcgcctg caaactgaat taaataaacc agaatgacca gcaataccag taccgtattc
    13501 atcaccgtcg cgttgtagcc gatgtagcca tactgatagc caatctgacc taaaccaccg
    13561 gcaccgactg caccacccat cgcggaataa ccgaccaggg taatcagggt gatagttgcc
    13621 gcattcacca gacccggcaa cgcttccggt aacagcactt tacagacgat ctgcattggc
    13681 gtggcaccca ttgcgcggga agcttcaatt aacccggttg ggatctccag cagagcgttc
    13741 tcgaccatac gggcaataaa cggtgctgca ccaacggtta acggaacaat tgctgcctgc
    13801 aaaccaatcg atgtaccgac aataacgcgg gtaaacggaa tcatccatac cagcaagata
    13861 atgaacggga tggaacggaa aatgttcaca atcgcagaaa cggtacgata cagcttcgcg
    13921 ttagcaataa tttgccccgg acgcgtgaca taaagcagaa cgccaaccgg cagaccaatc
    13981 acaaacccaa aaaatccgga tacgaaggtc attgccagcg tttcccatac gccacgaacc
    14041 agcagccaca tcatcggctc agacataacc cagtacctct acttttacat ggtgttcctg
    14101 cagccaggca atggcggctt gcgtatcttg ttgtgtgccg tgcatttcag tcagcatgat
    14161 gccgaattta acgccaccgg cgtagtccat ctgcgcgcta ataatgttgt tgttgacgtt
    14221 gaaacgacgc gcggtttcag aaagcagtgg ggcatcgacc gattgaccgg taaactccag
    14281 acgcagcatc ggcacgcagt cagtaaatgg ctccgtttgc agacgttcct ggtaatcttc
    14341 cgggatatcc agatgcaggg tcgactgaat aaacttctgc gccagcggcg ttttcggatg
    14401 cgagaacact tcacttaccg tgtcctgctc gatcagttcg ccattgctga tgaccgccac
    14461 gcaatcacaa atacgcttca caacgtccat ttcgtgggtg atcaacagaa tcgtcaaacc
    14521 cagacggcgg ttaatgtctt tcagcagttc gagaatagaa cgtgtcgttg ccgggtccag
    14581 cgcgctggtg gcttcatcac acagcaatac tttgggattg ctggctaatg cacgggcaat
    14641 cgccacacgt tgtttctgcc caccggaaag attcgacggg tagctatcat gcttatcgcc
    14701 aagaccaacc aatgacagca attccgtcac gcgacgtttg atctcgtctt tcggtgtgtt
    14761 gtccagctcc agcggcagag ccacgttgcc aaaaacagta cgcgaagaga gcaggttaaa
    14821 atgctggaaa atcataccaa tctggcggcg agctttggtc aactcggatt ctgacagcgt
    14881 ggtcaattcc tggccatcga ccagcacgct accctcggtt gggcgctcca gcaagtttac
    14941 acaacgtata agcgtactct tacccgcgcc tgaggcaccg ataacgccat aaatttgtcc
    15001 agctggcaca tgcaggctga cgttgttcaa cgcctggatg gtgcgggtgc cctggtggaa
    15061 cactttggtg atattcgaaa gttttatcat tgattattta ttatcgtcat taagttagtc
    15121 gtagcatctc gaatgcctga aacgggcaac gccgtcaatg aaatggatgt taaggcatcc
    15181 agacgtctaa atcaatcagg tttatgcgaa gagcactttc ttgcaggtcg aaacatgcga
    15241 tactagcgtc ccatgcctta ttaaggagct ataaaaggtg gcgaagagcg tacccgcaat
    15301 ttttcttgac cgtgatggca ccattaatgt cgatcacggc tatgtccatg agatcgacaa
    15361 ctttgaattt atcgacggtg ttattgacgc catgcgcgag ctaaaaaaaa tgggctttgc
    15421 gctggtggta gtaaccaacc agtctggcat tgctcgcggt aaatttaccg aagcacagtt
    15481 tgaaacgctg accgagtgga tggactggtc gctggcggac cgagatgtcg atctggatgg
    15541 tatctattat tgcccgcatc atccgcaggg gagtgttgaa gagtttcgcc aggtctgcga
    15601 ttgccgcaaa ccacatccgg ggatgctttt gtcagcacgc gattatttgc atattgatat
    15661 ggccgcttct tatatggtgg gcgataaatt agaagatatg caggcagcgg ttgcggcgaa
    15721 cgtgggaaca aaagtgctgg tgcgtacggg taaacctatt acacctgaag cagaaaacgc
    15781 ggcagattgg gtgttaaata gcctggcaga cctgccgcaa gcgataaaaa agcagcaaaa
    15841 accggcgcaa tgattaaaag atgaacggtt gaaataaaaa tgcatttttc cgcttgtctt
    15901 cctgagccga ctccctataa tgcgcctcca tcgacacggc ggatgtgaat cacttcacac
    15961 aaacagccgg ttcggttgaa gagaaaaaat cctgaaattc agggttgact ctgaaatagg
    16021 aaagtgtgat atacgccacc tcgcgacagt gagctgaaag ctgcgtcgca actgctcttt
    16081 aacaatttat cagacaatct gtgtgggcac tcgaagatac ggattcttaa cgtcgcaaga
    16141 cgataaatga ataccaagtc tcaagagtga acacgtaatt cattacgaag tttaattctt
    16201 tgagcatcaa acttttaaat tgaagagttt gatcat
//
LOCUS       DADLTY010000091        15930 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.091, whole genome shotgun sequence.
ACCESSION   DADLTY010000091 DADLTY010000000
VERSION     DADLTY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.091"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="JN334_002499"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="JN334_002499"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAY7438606.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="JN334_002500"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="JN334_002500"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAY7438607.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="JN334_002501"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="JN334_002501"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAY7438608.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="JN334_002502"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="JN334_002502"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAY7438609.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4817
                     /gene="citX"
                     /locus_tag="JN334_002503"
                     /pseudo
     CDS             4267..4817
                     /gene="citX"
                     /locus_tag="JN334_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4792..5670
                     /gene="citG"
                     /locus_tag="JN334_002504"
     CDS             4792..5670
                     /gene="citG"
                     /locus_tag="JN334_002504"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAY7438610.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5721..7183
                     /gene="citT"
                     /locus_tag="JN334_002505"
                     /pseudo
     CDS             5721..7183
                     /gene="citT"
                     /locus_tag="JN334_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7297..8103
                     /gene="rna"
                     /locus_tag="JN334_002506"
     CDS             7297..8103
                     /gene="rna"
                     /locus_tag="JN334_002506"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HAY7438611.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8332..8742
                     /gene="rnk"
                     /locus_tag="JN334_002507"
     CDS             8332..8742
                     /gene="rnk"
                     /locus_tag="JN334_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HAY7438612.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8973..10211)
                     /locus_tag="JN334_002508"
     CDS             complement(8973..10211)
                     /locus_tag="JN334_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HAY7438613.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10432..10860
                     /gene="uspG"
                     /locus_tag="JN334_002509"
     CDS             10432..10860
                     /gene="uspG"
                     /locus_tag="JN334_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HAY7438614.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10981..12546)
                     /gene="ahpF"
                     /locus_tag="JN334_002510"
     CDS             complement(10981..12546)
                     /gene="ahpF"
                     /locus_tag="JN334_002510"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAY7438615.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12791..13354)
                     /gene="ahpC"
                     /locus_tag="JN334_002511"
     CDS             complement(12791..13354)
                     /gene="ahpC"
                     /locus_tag="JN334_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HAY7438616.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13726..14472
                     /gene="dsbG"
                     /locus_tag="JN334_002512"
     CDS             13726..14472
                     /gene="dsbG"
                     /locus_tag="JN334_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAY7438617.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14682..15584
                     /locus_tag="JN334_002513"
     CDS             14682..15584
                     /locus_tag="JN334_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7438618.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15731..15930
                     /locus_tag="JN334_002514"
                     /pseudo
     CDS             15731..15930
                     /locus_tag="JN334_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3954 a         4118 c         3854 g         4004 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ccacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgcgcc tcgccgctgc ctgttgtgcg agcaaagcgc agccgtctgc gcgcgtggaa
     4741 aaacccatca actgaccgat ttactcaacc gcatggaggc actgctgaac gatgtcgatg
     4801 cctgcaacgt caactaaaac cacaaagctt gcgacgtcat taatcgatga gtacgccctg
     4861 ctgggctggc gcgccatgct gactgaagtc aatctgtcac cgaaaccagg cctcgtggat
     4921 cgcattaact gcggtgcgca caaagatatg gcgctggaag atttccaccg cagcgcgctg
     4981 gcgattcagg gctggttacc ccgtttcatt gaatttggtg cctgtagtgc agaaatggca
     5041 ccggaagcgg tactccacgg attacgccca attggtatgg cttgcgaagg tgatatgttc
     5101 cgcgccactg cgggcgtaaa cacgcataaa ggcagcattt tttctttagg gctgctatgt
     5161 gcggcaattg gccgtttgct tcagctcaac cagccggtta cgccaacaac cgtttgttct
     5221 acggcggcaa gtttctgccg tggcctgacc gatcgcgaac tgcgtaccaa taattcacga
     5281 ctgacggcag gtcaacggtt gtaccagcag cttggcctta ccggcgcacg cggtgaagcc
     5341 gaagcaggtt atccactggt gatcaaccac gccttgccgc attacctcac tctgctggat
     5401 caggggttag atcctgaact ggcattgctc gataccttgc tcctactgat ggctaccaac
     5461 ggcgatacca acgttgcatc gcgcggtggc gaggggggcc tgcgctggct acagcgcgag
     5521 gcgcaaacat tattgcaaaa agggggcatt cgaaccccca ccgatctcga ttatctccgg
     5581 cagttcgaca gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc cgacctgctg
     5641 atccttacct gttttttagc acagatttaa ttatttaagc acttgataaa tttggaaata
     5701 ttaattttcg gagaacccgt atgtctttag caaaagataa tatatggaaa ctattggccc
     5761 cactggtggt gatgggtgtc atgtttctta tccctgtccc cgacggtatg ccgccgcagg
     5821 catggcatta cttcgctgtg tttgtggcaa tgattgtcgg catgatcctc gagccaattc
     5881 cggcaacagc gatcagtttt attgcggtta ctatttgcgt tattggcagt aattacctgc
     5941 tctttgatgc caaagaatta gctgacccag cgtttaatgc gcaaaaacag gcgctgaaat
     6001 ggggcctggc tggtttttcc agcaccacgg tatggctggt atttggcgca tttatttttg
     6061 cattagggta tgaagtttcc gggttaggtc gtcgcattgc ccttttcctg gtgaaattca
     6121 tggacaaacg cacgctgacg ttgggttatg cgattgtcat tatcgacatt ctgctggcac
     6181 cgtttacacc gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc attaaaaacc
     6241 tgccgccgtt gtttaaatca ttcccgaacg atccgtccgc gcgtcgtatt ggcggctatt
     6301 tgatgtggat gatggtcatt agtaccagtc tgagttcgtc catgtttgtc accggtgcgg
     6361 accaaacgtg ctgggtctgg agttcgtcag caaaattgcc ggtatccaga ttagctggtt
     6421 gcagtggttc ctctgcttcc tgccggttgg ggttatcttg cttatcattg cgccgtggct
     6481 ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa cctgggcggg
     6541 tgatgaacta aaaaccatgg gtgcgctgac acgcagagag tggacgctga ttggccttgt
     6601 attgctcagc ttaggtttgt gagtatttgg cagtgaagtc attaatgcta ctgcggttgg
     6661 tctgctggca gtttcgctaa tgctggctct gcacgttgtg ccgtggaaag acattacccg
     6721 ctataacagc gcatggaaca cgctggtcaa cctggcaact ctggttgtga tggctaacgg
     6781 cctgactcgt tctggtttta ttgactggtt cgccggtacc atgagtacgc acctggaagg
     6841 attctcacca aacgcaacgg tgattgtact ggttctggtg ttctactttg cacactacct
     6901 gtttgccagc ctgtctgcgc acaccgcaac catgctgccg gttattctgg ccgtcggtaa
     6961 aggtattccg ggcgtaccaa tggaacaact gtgtatcctg ctggtgctgt ctatcggtat
     7021 catgggctgt ctgacgccgt atgcaaccgg tcctggggtg attatttacg gctgtggcta
     7081 tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc ggggtgattt acatctctat
     7141 gttgctgttg gttggctggc cgattctcgc catgtggaac taatacgttc gccaaacgct
     7201 gaataaatca tcacgcccgc caggtaagcc cacctggcgg gcttttttat gatttaatag
     7261 atagtattca gtgtatgtat gagttccaca cccattatga aagcattctg ccgtaacgcc
     7321 gcgttgctcg cggtttctct gcttccctta tcttctgcca acgccgtagc gttgcaggca
     7381 aaacagtatg gcgattttga tcgctatgtc ctggccctct cctggcaaac cggattttgc
     7441 cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat gtcgcctgca aaccgaaacg
     7501 accaacaaaa ctgattttct gaccgtacat ggtctgtggc caggattgcc taaatcggtt
     7561 gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg gttgcgctac tcgcccaatc
     7621 ccgaatctac cagaagcacg cgccagccga atgtgttcat cgccggaaac cgggttatca
     7681 ctggaaacgg ccgctaaact aagtgaagtc atgccaggag ctggcggacg ttcctgcctg
     7741 gaacgctacg aatatgccaa acacggtgcc tgctttggtt ttgatccgga cgcatacttc
     7801 ggtacgatgg tacgcctgaa tcaagaaatt aaagagagcg aagccggaaa attccttgcg
     7861 gataattacg gtaaaacagt gagccgccgt gactttgacg ccgcctttgc caaaagctgg
     7921 ggaaaagaga acgtgaaagc agttaagcta acgtgccagg gtaaccctgc gtatttgact
     7981 gaaattcaga tctcgatcaa agctgacgcc atcaacgctc cgctttctgc aaactcattc
     8041 ttgccacaac ctcacccagg taactgtggc aaaacctttg tgattgataa agcgggttat
     8101 taatcctctg atttacaaaa cacgctaatt cgtcttccat aaccatgccg attaagaatt
     8161 atacataatg cgctgcattg aggaattccc tgaatgtgac gcaaatcact tcaaagagtg
     8221 agcaacagtc agtatcatca tgtgagttaa accctcgccg cctgacggtg agggttttct
     8281 tttgggtatg tttatctgcg acactcgcag taccgacgac atggagtaaa aatgtccaga
     8341 ccaactatca tcattaacga cctggatgcc gaacgcatcg atattctgct ggagcaaccc
     8401 gcctatgctg gtttgccaat cgccgacgcg ttaaacgcag agttggatcg cgcccaaatg
     8461 tgttcgccag aagagatgcc acacgacgtg gtgacaatga acagccgggt taaattccgc
     8521 aatcttagcg atggcgaagt gcgtgtgcgc acgctggtgt atccggcaaa aatgaccgat
     8581 agcaatactc agctttccgt tatggctccg gtaggtgccg cactgctggg gctgcgcgtt
     8641 ggcgattcca ttcactggga acttccgggc ggcgttgcaa cccaccttga agtgctggaa
     8701 ctcgaatacc agccagaagc tgctggcgac tacctgcttt aatcccatct gcacaagcga
     8761 taactcgctt tgcccagagg atgcactcgc atcctcttta cgcaaaatca gcaatttttc
     8821 ctggttctca ctgttcactt ttgctgcaaa attattccgt ttcgtttgtg ggaataacca
     8881 tggcagaagc attttacatc ctgataggat ttctgattat ggcggcgatt atcgtcatgg
     8941 ctgtccttta cctcgaaaac cactcctgac gatcatattg ttccccccgg catcgcattc
     9001 accagacctg aaaccgcctt ctgcgccgcc tctgcgcttt gtgcaccggg taccagaatc
     9061 accttacggc actcctcttc acgtttttcg aaaatctcat atccccgggc ggcctcttca
     9121 aacggcatat agtgggtaac aatttcttct ggtttcagta atcctttctc aattaacggt
     9181 aataattctc ccagccatgc gtgaacgtgg gtctgtccca ttttaaacgt caacccttta
     9241 tcaaaggcgt cgccaaacag gaaaccgtga ataaatccag cgtagacgcc cggtacgcta
     9301 acaatgccgc cacgcctgac cgccgcaata cactggcgca acgctttacc gctgctgccc
     9361 tccagtttca ggttagtcag caccgtttcc gtggtgctgc ctttcgcttc aaaaccgacg
     9421 gcgtctatta ctgcatccac gccccggtga cctgccgttt gttcaataat tgactgtgcc
     9481 ggatcgctat cttcatcgaa attaatcggg atcgcgccgt agcggtcagc ggcgaaacgc
     9541 aagcggtagg gatgatgatc aacaacaaaa atctgttccg caccgagcaa ccgtgcacag
     9601 gcgattgtca acaatcccac aggaccagca ccatagactg caacgcttga accttgttgg
     9661 atctgcacat tttttgctgc ctgccatgcc gttggcagaa tatcagaaag gaaaagggct
     9721 ttatcatctg aaagcaaagg cggtacttta aacggcccca cattcccttt cgggacgcgg
     9781 acatattccg cctgcccgcc aggaacgcca ccatacaggt gactataacc aaacaatgcc
     9841 gcgggagcag gtatctgttt tttattgagc gcagcgcctt tacccgcatt ggtattttcg
     9901 caggcggcat attgctgtaa tcgacagaaa aaacagtcgc cacaagcaat gacgaacgga
     9961 attaccaccc ggtcgccttt ttgcaaattt tttacgtcct tcccggtttc aacgacttcc
    10021 cccataaatt catgaccaaa aatatcgcca tgtttaacct ggggtatttt gcctcgataa
    10081 agatggaggt cagagccaca gatcgccgtt accgtaatac gcagaataat atcatctgcc
    10141 tgttcaatgc ccggatcggg aacattttct acctgaacgt gatgtgggcc gtgataagtc
    10201 aatgctttca tgcaacctcc agagacagtt caaaaaagag tcagtcttta agggtagcta
    10261 tcacagcggg aaaacgggcg gtaccgcagg ttttgggaga attcctagtg ttcagtgaga
    10321 atatattaat cacagaaacc gttcgtaagg aaaaagaacc agacaaatat cagggataaa
    10381 tattataatc atggagaatg atttgttcat gattaacagg gagaaagggt tatgtataag
    10441 gcaatcatta tgccagttga tgtatttgaa atggaattga gcgacaaagc tgttcgccac
    10501 gctgaattcc tcgcccagga tgacggagtt attcatctac ttcacgtact acccgggtca
    10561 gccagcctga gcctgcaccg ttttgccgct gatgtgcgtc gttttgaaga gcatctgcaa
    10621 catgaagcag aagaacgtct gcaaacgatg gtcagccact tcaccatcga tccttcccgc
    10681 attaaacaac atgtccgttt tggtagcgtg cgggatgaag tcaatgagtt ggcagaagaa
    10741 ctgggggctg atgttgtagt tatcggttct cgcaacccat cgatttcgac ccatctgtta
    10801 ggttctaacg cctcgagcgt gatccgccac gccaatctgc ctgtactggt cgttcgctaa
    10861 cagataaaag tatttatgcc gccctgcctg gaacatcccg gcagggcttt ttaatgccat
    10921 aaaaaagccg ccaggtttga ccccggcggc taagcaattg caggtgaatc ttacttcttc
    10981 ttatgcagtt ttggtgcgaa tcaggtagtc aaaagcactc agagaggctt tggcaccttc
    11041 gccagtggcg atgatgatct gcttgtacgg aaccgtcgta cagtcacccg ctgcgaacac
    11101 gcctttgacg ttggtttcac atttcgcatc aatgataatc tcgcccatgc ggttacgttc
    11161 gactgcgcct tccagccagt tggtgttcgg cagcagacca atctggacaa aaataccggc
    11221 cagttcgatg ttgtgaatat tgccgctgac acgatctcga tattccagac caacgacttt
    11281 gctgccgtcg cctttcactt ccgtggtttg cgcattcaga ataatgtcga cgtttttcag
    11341 gctgcgcagt ttgtcctgca gaacctggtc ggctttcatt tctggcgcaa attccagcag
    11401 cgttacgtgc tcaacgatac ccgccaggtc gattgccgct tccacgccgg agttaccgcc
    11461 gccgataacc gctacgcgtt tgcctttaaa cagcgggccg tcgcagtgcg ggcagtaggt
    11521 cacgccttta gtgcgatact gatcttcgcc cggaacgttc atgttgcgcc attttgcacc
    11581 cgtcgccacg ataatgctgc gtgctttcag taccgcgcca gaagctgttt caatctggtg
    11641 tagaccacct tcaaccgctg ccgggatcag tttgctggcg ctctggctgt cgatcacatc
    11701 aacgtcgtat tcatcaacgt gaactttcag tgcgcctgcc aacttctgcc cttcagtctt
    11761 cggtacagaa atgtagtttt cgatatcaac ggtatcgagg atctgaccac caaaacgttc
    11821 gcccatcaga ccggtacgga tgcctttacg tgcggagtaa attgctgccg ctgcacccgc
    11881 cgggccggaa ccaacgatta atacgtcata agcatcacgc ttgttcagct cttctgccgc
    11941 acgattttcc gcgccagtat caattttggc aacgatttca gtcaacgtca tgcggccctg
    12001 accaaactct ttcccgttta cgaacactgc cggaacgccc atcacgttgc gatcggtgat
    12061 ttcgttctgg aaggtgccgc cgtcaattgc agtgtgcttg atgcgcgggt tcaatacgct
    12121 catcaggttc agcgcctgca ccacgtccgg gcagttatgg caagagagcg agtaataggt
    12181 ttcgaattca aaatcaccgt caatatggcg aatctgctcc agcagagact gcgcttcttt
    12241 cgacggatga ccaccggtcc acagcaacgc cagtaccagc gaggtgaact cgtggcccag
    12301 cggggagcct gcaaaacgtg gtccctggtt ggaacctggg ttggtgatca ggaaagacgg
    12361 cttacgcact ggcaagctgt tatcttcttt aaaggtgact ttgtctgaca gttctgcgat
    12421 ttcagccaac agttccttga tttctgccga tttagcgctg tcatccagcg tggcaattaa
    12481 ctcaacaggc ttggtcaatt tctcaaggta agccttgagt tgagttttca tatttgtgtc
    12541 gagcatgttt atctcctggg ctttaaacat catcatgcaa gcggcctctg cggctacctg
    12601 gatgcagctt gcatcatggt gcggggaaga aaatcgggtg ccgcagcacc cgaagaatta
    12661 ggtgaatttc cgaagtattt cgcgtcagag caaggcgact agcctgtgaa tccccgggag
    12721 cttacacaag taagtgaccg gggtgagcgg gcgacgccaa cgccgctatg gcgtgaaaga
    12781 cgaaggaaat ttagatttta ccaaccaggt ccagagacgg agccagagtt gcttcacctt
    12841 ctttccattt agccgggcaa acttcacctg ggtgagaagc tacgtactga gctgctttga
    12901 ttttacgcag cagatcagac gcgtcacggc caatgccttc agcggtaact tcgattgcct
    12961 ggatgatacc ctgcgggtca acaacgaagg tcgcacggtc agccagacct tcatcttcac
    13021 gcatgttgtc gaagttacgg gtcagggcgc cagtcgggtc gccgatcatc gcatatttga
    13081 ttttagcgat ggtttcagag ctgctgtgcc atgctttgtg ggtgaagtga gtatcggtag
    13141 atactgcgta tacgtctacg cccagtttct gcagttcttc gtagtggtca gcaacgtcac
    13201 ccagttcggt cgggcatacg aaagtaaagt cagccgggta gaagaagaag acgctccagc
    13261 ggccttcggt atctttttcg gtgatttcga tgaattcgcc gtttttgaat gcctggtttt
    13321 taaaaggttt aattttggtg ttaatcaagg acatctatac ttcctccgtg ttttcgatga
    13381 gatgtaaggt aaccaatttt tgccgattcg gctaatgcgt ttccattatc aaatcgataa
    13441 gttttacctt acaaccttcg taagacaact ttgtgaactt tgcaccgcca gcggcgataa
    13501 aaagtaaaat ggcccccttt ccaggtgacc gtgtaatctg aactaccctt tggtatcttc
    13561 agcgcctgcc tgcaggctga ggtgttgccc ttacaaatgc aacaacgaca tggattacaa
    13621 caccctcata aacaaagggc aatcacctga tctaagctct tacctatgac agtgataggt
    13681 tatgcctttt actcgacttt tgcactgact gaaaagaaca aattaatgtt aaaaaagata
    13741 cttttactgg ctctgcttcc tgcaatcgcc ttcgcagagg aacttccttc cccagtaaaa
    13801 gcgattgaaa aacagggcat tacaatcatc aaaacgttcg atgcccccgg aggaatgaaa
    13861 ggttatctcg gaaagtatca ggatatgggc gtcaccatct acctgactcc cgatggtaag
    13921 cacgctatct ctggttacat gtacaacgag aaaggtgaaa acctgagtaa cacacttatc
    13981 gaaaaagaaa tttacgcacc agccggacgc gaaatgtggc aacggatgga acaatcccac
    14041 tggctcctcg acggtaaaaa agatgcgccg gtcattgtct acgtcttctc cgatccgttc
    14101 tgcccatatt gtaaacagtt ctggcagcag gcgcgcccgt gggtagattc tggcaaagtg
    14161 caattaagaa cattgttggt tggggttatc aagccagaaa gcccggcgac agcagcggca
    14221 attcttgcct ccaaagatcc cgcaaaaacc tggcaacaat atgaagcctc tggttgcaag
    14281 cttaagctaa acgtgcctgc aaacgtaagt acagagcaaa tgaaagtgtt aagtgacaat
    14341 gagaaactga tggacgatct gggggcaaat gtcacgccgg ctatctatta catgagtaag
    14401 gaaaatacgc tgcaacaggc cgtggggttg cccgatcaga aaacgcttaa tatcattatg
    14461 ggaaataaat aaaaggtaat tcatggatgg ttgaactata tccctgcttt tcaaccatcc
    14521 taaaaaagca tgaatttctc gctctaccct aagtggctgt gctattttag aaaaacttaa
    14581 tcctgagtta agcaaaaaaa cataatcaat aaaaatacat acgcaaaaaa cattgattaa
    14641 gtgaatatac catggaagaa aaatataaac cggagtagtg tatggccaat ctctacgact
    14701 tgaaaaagtt cgacttaaat cttcttgtca tatttgagtg tatttaccag catttaagca
    14761 ttagcaaagc tgctgaatca ctatatatca ccccatcagc cgtgagccag tcgttacaac
    14821 gcttacgtgc gcaatttaat gaccctttat tcattcgctc aggaaaaggt atcgcgccga
    14881 cgacaacagg gttgaatctg catcaccatc ttgagaaaaa cctcaaaggc cttgaacaaa
    14941 caattaatat agtcaataag tccgaactaa agaaaaactt tattatctac ggccctcagc
    15001 tcatttcttg ctctaataat agtatgctga tccgctgcct acggcaagat gcctctgtcg
    15061 agattgagtg ccatgatatc ctcatgtcag cagagaatgc tgaagaactg ttggttcacc
    15121 gcaaagccga tctggtgatc acccaaatgc cagtgatcag ccgttccgtc atttgcatgc
    15181 cattgcatac aattcgcaat actctaattt gtagtaataa gcatccgcga attactgaca
    15241 atagcactta tgaacaaatt atggcagaag agttcaccca attaatagcc aaatcagcag
    15301 gagttgatga tattcaaatg gaaatagacg agaagtttat gaatcgcaaa atttcatttc
    15361 gtggttcctc tttgctgaca ataattaata gcattgcagt tacagattta ctgggaatag
    15421 tcccctatga actttataac taccaccgtg actttcttaa tttaaaagag ataaaactag
    15481 aacatccgtt accttcaata aagctttaca tttcctataa taaatcttct ttaaataatt
    15541 tggtgttctc ccgatttatt gaccgtctaa atgagagttt ttgatatata actatagaca
    15601 gctactataa ctttcatcta tttattcaca gcgcgaatga aaaataaaac actctgcttt
    15661 acagtgttta ctttataatt aacagtctta tctcactgtt caccctgtaa cgttcagaga
    15721 gcgtcttcag atgtctattt ataaaattcc tcttccgctc aatattctgg aagctgcacg
    15781 agagcgtatt acctagacac tcaacaccct accccgcgtt tgtgtctcat tttctggcgg
    15841 caaagactct ggtttaatgc tgcatctgac agccgaactt gcccgacaaa tgggcaaaaa
    15901 aatctgcgtt ttgtttatca actgggaggc
//
LOCUS       DADLTY010000092        15930 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.092, whole genome shotgun sequence.
ACCESSION   DADLTY010000092 DADLTY010000000
VERSION     DADLTY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.092"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(1..687)
                     /locus_tag="JN334_002515"
                     /pseudo
     CDS             complement(1..687)
                     /locus_tag="JN334_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(1004..1828)
                     /gene="iclR"
                     /locus_tag="JN334_002516"
     CDS             complement(1004..1828)
                     /gene="iclR"
                     /locus_tag="JN334_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HAY7438619.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            2028..5711
                     /gene="metH"
                     /locus_tag="JN334_002517"
     CDS             2028..5711
                     /gene="metH"
                     /locus_tag="JN334_002517"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAY7438620.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            5931..7562
                     /locus_tag="JN334_002518"
     CDS             5931..7562
                     /locus_tag="JN334_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAY7438621.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7653..8342)
                     /gene="pepE"
                     /locus_tag="JN334_002519"
     CDS             complement(7653..8342)
                     /gene="pepE"
                     /locus_tag="JN334_002519"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HAY7438622.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8724..9953)
                     /locus_tag="JN334_002520"
     CDS             complement(8724..9953)
                     /locus_tag="JN334_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7438623.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            complement(10005..10793)
                     /locus_tag="JN334_002521"
     CDS             complement(10005..10793)
                     /locus_tag="JN334_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAY7438624.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            complement(10804..11601)
                     /locus_tag="JN334_002522"
     CDS             complement(10804..11601)
                     /locus_tag="JN334_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY7438625.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            complement(11667..12160)
                     /locus_tag="JN334_002523"
                     /pseudo
     CDS             complement(11667..12160)
                     /locus_tag="JN334_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(12160..12567)
                     /locus_tag="JN334_002524"
     CDS             complement(12160..12567)
                     /locus_tag="JN334_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HAY7438626.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            complement(12577..13383)
                     /locus_tag="JN334_002525"
     CDS             complement(12577..13383)
                     /locus_tag="JN334_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7438627.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(13453..14400)
                     /locus_tag="JN334_002526"
     CDS             complement(13453..14400)
                     /locus_tag="JN334_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY7438628.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            14748..15620
                     /gene="rluF"
                     /locus_tag="JN334_002527"
     CDS             14748..15620
                     /gene="rluF"
                     /locus_tag="JN334_002527"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HAY7438629.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(15753..15926)
                     /locus_tag="JN334_002528"
     CDS             complement(15753..15926)
                     /locus_tag="JN334_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HAY7438630.1"
                     /translation="MTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEAS
                     FSWSANTSTRGRR"
BASE COUNT         3834 a         4071 c         4142 g         3883 t
ORIGIN      
        1 ttgattccat cttgtcaaaa tatgctttca taactagccc gaaatcaccg caggcagcaa
       61 cgcaacgccc ctcaggacgg ctggaaatca acgccaaagt ctggtttttg cattttttgt
      121 tgaagtgagt gagaaatttt tcagctgaag agtaatcatc ttgatggggt ttgtaatcaa
      181 cattctgata aatatcatta atatattgtg cagcaatgtg tcggtatact atacggtttt
      241 ggggcgcccc gggaaataac attgaccatt aagtttaatt cttgggaatt ctttttcttt
      301 tctatctgat ttttggttta attcttgttt atcaagagtg tctgagcgtt gaggtaaaat
      361 ttccattagg tattcttcaa atattgtagc aatggtttga gtgagtgtcg tgttttcgtt
      421 tttattaatc atcgtaattt ctttcttatc agcttgtaag acggacgtgg aaaatttaat
      481 gacaaaatcc ttgttgctaa agatatgacg aacattttgt ctacagttct ctgagaagct
      541 ttttaataga ggcgtcgcca gctccttgcc agaaaattta tcctcgagtt ctttataaaa
      601 caattcactc agggtttggt gttcatttgt ctgggctgta ttattaatat ttgcagagaa
      661 agaactacga ggaatacgag taatcattta aatatctcat tgtttattga tgtcttgaat
      721 tttaactatc agaacagtga aaaaatttaa tatgattaca actaaaaaaa tatcataaat
      781 cgctcaatct cataatgcag ccgtaaaagt ttcggtggaa tgagatcttg cgattttgtt
      841 aataattaag tggtttgatg tttaaaaata atgctgagtt aataaaagtg tgtgaagtgt
      901 atgacattaa gttattttgg cgtcaatgcg attaacagac acccttattc tattgccact
      961 caggtatgat gggcagaata ttgcctctgc ccgccagaaa aagtcagcgc attccaccgt
     1021 acgccagcgt cacttccttc gccgctttaa tcaccatcgc gccaaactcg gtcacgcgat
     1081 catcggtaat acgtgaaatc ggtccggaaa tagaaattgc ggcaaacggt tcgcggtgct
     1141 catcgaaaat acacgctgca aggcaacgta gccccagcgc atgttcctca tcgtcaaatg
     1201 aataaccccg tttgcgcgtt tgggcgagat cttcttttaa atgcacagga gacaccagag
     1261 ttgcgtgggt ataggcatgt aaccctttgc ggtgcagcag cttcgtcacc tgttcttcgc
     1321 tcagttgggc taaaaaggct ttacccgcac cggaagcgtg catcggcaat ttaccgccga
     1381 taggcgcgga cattcgcatc aggtgggtac actgtacctg gtcgataata atcgcttcgt
     1441 gatcgctttg atcaagcacc gccatattga ccgtttcgcc agactcttcc attagattgc
     1501 gcaggatagg gtgaacaatt gctaacaaat tacggctctg gagaaagctg ctgccgacca
     1561 taaaggcatg tgcgccgatt gcccagtgtc ccagttcgcc gacctgacgc acgaaaccct
     1621 gctgttgcat cgtggttagc aggcggtggg tcgtggaatt gggtaacccg gcttgttgcg
     1681 ccagctctgt gagtgccacg ctgccattgg attcggcaat ccactccagt aatttcaggc
     1741 cacgcgttaa agactgaacc tgtccagtcg ctggtgcggt ggcaacggcg ggttttctgc
     1801 cgcgtttcgc gggaatgggt gcgaccatga cagcctcctt tttctgtatc gtggaaatta
     1861 ttttcatttt tattgttagc taatgcaata gttgctgaac tgatccgatg agttaatgtt
     1921 gaacaaatct catgttgcgt ggtggtcgct tttaccacag atgcgtttat tccagtatgg
     1981 tttgttgaat ttttattaaa tctgggttaa gcgtgtcggg agcaagtgtg agcagcaaag
     2041 tggaacaact gcgtgcgcag ttaaatgaac gtattctggt gttggacggc ggtatgggca
     2101 ccatgatcca gagctatcga ctgaacgaag ccgattttcg tggtgaacgc tttgccgact
     2161 ggccatgcga cctcaaaggc aacaacgacc tgctggtact cagtaaaccg gaagtgatcg
     2221 ccgctatcca caacgcctac tttgaagcgg gcgcggatat catcgaaacc aacaccttca
     2281 actccacgac cattgcgatg gcggattacc agatggaatc cctgtcggcg gaaatcaact
     2341 ttgcggcggc gaaactggcg cgcgcctgtg ctgacgagtg gaccgcgcgc acgccggaga
     2401 aaccgcgcta cgttgccggt gttcttggcc caaccaaccg cacggcgtct atttctccgg
     2461 acgtcaacga tccggcattc cgcaacatca cttttgacca gctggcggcg gcctatcgcg
     2521 aatccaccaa agcgctggtg gaaggtggtg cggatctgat cctgattgaa accgttttcg
     2581 acacccttaa cgccaaagcg gcggtctttg cggtgaaaac ggagtttgaa gcgctgggcg
     2641 ttgagctgcc gattatgatc tccggcacca tcaccgacgc ctccgggcgc acgctctccg
     2701 ggcagaccac cgaagcattt tacaactcat tgcgccacgc cgaagcgctg acctttggcc
     2761 tgaactgtgc gctggggccc gatgaactgc gccagtacgt gcaggaacta tcacggattg
     2821 cggaatgcta cgtcaccgcc cacccgaacg ccgggctgcc taacgccttt ggtgaatacg
     2881 atctcgacgc cgacacgatg gcaaaacaga tacgtgaatg ggcgcaagcg ggctttctca
     2941 atatcgtcgg tggttgctgc gggaccacgc cgcaacatat tgcagcgatg agtcgtgcag
     3001 tagaaggatt agcgccgcgc aaactgccgg aaattcccgt agcctgccgt ttgtctggcc
     3061 tggagccgct gaacattggc gacgatagcc tgtttgtgaa cgtgggtgaa cgcaccaacg
     3121 tcaccggctc tgctaagttc aagcgcctga tcaaagaaga gaaatacagc gaggcgctgg
     3181 atgtggcgcg tcagcaggtg gaaaacggcg cgcagattat cgatatcaac atggatgaag
     3241 ggatgctcga tgccgaagcg gcgatggtgc gttttctcaa tctgattgcc ggtgaaccgg
     3301 atatcgctcg cgtgccgatt atgatcgact cctcaaaatg ggatgtcatt gaaaaaggtc
     3361 tgaagtgtat ccagggcaaa ggcattgtta actctatctc gatgaaagag ggcgtcgacg
     3421 cctttatcca tcacgcgaaa ttgttgcgtc gctacggtgc ggcagtggtg gtgatggcct
     3481 ttgacgaaca gggacaggcc gatactcgcg cacggaaaat cgagatttgc cgtcgggcgt
     3541 acaaaatcct caccgaagag gttggcttcc cgccagaaga tatcatcttc gacccgaaca
     3601 tcttcgcggt cgccaccggt attgaagagc acaacaacta cgcgcaggac tttatcggcg
     3661 cgtgtgaaga catcaaacgc gaactgccgc acgcgctgat ttccggcggc gtatctaacg
     3721 tttctttctc attccgtggc aacgatccgg tgcgcgaagc cattcacgca gtgttcctct
     3781 actacgctat tcgcaatggc atggatatgg ggatcgtcaa cgccgggcaa ctggcgattt
     3841 acgacgacct acccgctgaa ctgcgcgacg cggtggaaga tgtgattctt aatcgtcgtg
     3901 acgatggcac tgagcgttta ctggagcttg ccgagaaata tcgcggcagt aaagcggacg
     3961 acactgctaa cgcccagcaa gcggagtggc gctcgtggga cgtgaataaa cgtctggaat
     4021 actcgctggt caaaggcata accgagttta tcgagcagga tactgaagaa gcccgccagc
     4081 aggctacgcg cccgattgaa gtgattgaag gcccgttgat ggacggcatg aacgtggtcg
     4141 gcgatctgtt tggcgaaggc aaaatgttcc tgccacaggt ggtcaaatcg gcgcgcgtca
     4201 tgaaacaggc agtggcgtat ctcgaaccgt ttattgaagc cagcaaagag cagggtaaaa
     4261 ccaacggcaa gatggtgatc gccaccgtga aaggcgatgt ccacgacatc ggtaaaaata
     4321 tcgttggtgt ggtgctgcaa tgtaacaact acgaaattgt cgatctcggc gttatggtgc
     4381 ctgcggaaaa aattctccgt atcgccaaag aagtgaatgc tgatttgatt ggcctttcgg
     4441 ggcttatcac gccgtcgctg gacgagatgg ttaacgtggc gaaagagatg gagcgtcagg
     4501 gcttcgccat tccgttactg attggcggcg cgaccacctc aaaagcgcac acggcggtga
     4561 aaatcgagca gaactacagc ggccctacgg tgtatgtgca gaatgcctcg cgcaccgttg
     4621 gtgtggtagc ggcgctgctt tccgatactc agcgtgatga ttttgtcgct cgtacctgca
     4681 aggagtacga aaccgtgcgt attcagcacg ggcgcaagaa accacgcaca ccaccggtca
     4741 cgctggaagc ggcgcgcgat aacgatttcg catttgactg gcaggcttac acgccgccgg
     4801 tggcgcaccg tctcggcgtg caggaagtgg aagccagcat cgaaacgctg cgtaattaca
     4861 tcgactggac accgttcttt atgacctggt cgctggccgg gaagtatccg cgcattctgg
     4921 aagatgaagt ggtgggcgtt gaggcgcagc ggctgtttaa agacgccaac gacatgctgg
     4981 ataaattaag cgccgagaaa acgctgaatc cgcgtggcgt ggtgggcctg ttcccggcaa
     5041 accgtgtggg cgatgacatt gaaatctacc gtgacgaaac gcgcacccat gtgatcaacg
     5101 tcagccacca tctgcgtcaa cagaccgaaa aaacgggatt cgctaactac tgtctcgctg
     5161 acttcgttgc gccgaagctt tctggtaaag cagattacat cggcgcattt gccgtgactg
     5221 gcgggctgga agaggacgca ctggctgatg cctttgaagc gcagcacgat gattacaaca
     5281 aaatcatggt gaaagcgctt gccgaccgtt tagccgaagc ctttgcggag tatctccatg
     5341 agcgtgtgcg taaagtctac tggggctatg cgccgaacga gaacctcagc aacgaagaac
     5401 tgatccgcga aaactaccag ggcatccgtc cggcaccggg ctatccggcc tgcccggaac
     5461 atacggaaaa agccaccatc tgggagctgc tggaagtgga aaaacatact ggcatgaaac
     5521 tcacagaatc tttcgcgatg tggcctggtg catcggtttc gggttggtat ttcagtcatc
     5581 cggacagcaa gtactacgcg gtggcgcaaa ttcagcgcga tcaggttgaa gaccatgccc
     5641 gccgtaaagg tatgagcgtc tccgatgttg agcgctggct ggcaccgaat ctggggtatg
     5701 acgcggactg attcacaaat ctgtcacttt tccttacaac aaacagggcg ctcaatgagt
     5761 gccctgtctc tttattaata tgaaacactt atactggaaa caggctggaa taatcttagc
     5821 cggaaaaacg aatgtcaaaa ggcacagtaa agatgccagt cagcgtgttt ccggtccttt
     5881 ggtcattcaa ttgcttcagg aacatataac gataaggaga atctgaactc gtgttaacgc
     5941 tgcttcacct gctttctgcc gtcgccctgc tggtctgggg gactcatatt gttcgaaccg
     6001 gcgtaatgcg cgtcttcggc gcgcgtttgc gtactgtcct tagccggagc gtcgaaaaaa
     6061 agccgctcgc cttttgcgcg gggatcggcg ttaccgcact ggtacagagc agtaatgcca
     6121 ccaccatgct ggtgacctcg ttcgtcgctc aggatctggt agccctcgca ccggctctgg
     6181 tcattgtgct gggggcagat gtcgggacgg cgctaatggc gcgtattctc accttcgact
     6241 tatcctggct gtcaccgtta cttattttta tcggcgtgat ttttttcctc ggacgcaaac
     6301 agtcacgcgc cgggcaactg ggccgcgtcg gtattggtct tgggctgatt ttgctggcgc
     6361 tggagttgat tgtgcaggcc gtaacgccga tcacccaggc aaatggcgtt caggtgatct
     6421 ttgcctcgct gaccggcgat attctgctgg atgcgctgat tggcgcgatg ttcgccatta
     6481 tcagctactc cagccttgct gctgtactgc tgaccgcgac tctgaccgct gcaggcatta
     6541 tctccttccc cgtggcgctc tgtctggtga ttggagctaa cctcggttcc ggcctgctgg
     6601 cgatgctcaa caacagtgcc gccaatgccg cagcccgccg tgtcgcgctg ggtagtctgc
     6661 tgtttaagct ggtgggtagc ctgattatcc tgccgtttgt ccatttgctg gcagagacaa
     6721 tggggaagct gtcattgcca aaagcggaac tggtgatcta tttccacgtc ttctacaacc
     6781 ttgtacgttg cctggtcatg ctgccatttg ttgacccgat ggcacggttt tgcaaaacga
     6841 ttattcgcga tgaaccggaa ctggataccc agctacggcc taaacatctg gatgtcagcg
     6901 cgctggacac gcccacgctt gctctggcga acgccgcgcg cgaaaccctg cgcattggcg
     6961 acgccatgga acagatgatg gaagggctga ataaagtgat gcacggcgag ccacggcagg
     7021 agaaagagct gcgtaagctg gcagatgata tcaacgttct ctacaccgcc attaagctgt
     7081 atctggcgcg gatgccaaaa gaagagttgg cggaggaaga gtcgcgccgc tgggcggaga
     7141 tcatcgaaat gtcgctcaac cttgaacagg cctccgatat cgtcgagcgc atgggcagcg
     7201 aaattgccga taaatcgctg gcagcacggc gggcattttc gcttgatggg ttgaaggaac
     7261 tggatgcgct ctatgagcaa ttgctcagta atttaaagct ggcaatgtcg gtgttcttct
     7321 ctggcgatgt caccagcgct cgtcgtttgc gtcgcagcaa acatcgtttt cgcattctta
     7381 atcgccgcta ttcccacgcc cacgtcgatc gcctgcatca gcaaaacgtg cagagcattg
     7441 aaaccagttc gctacattta ggcttactgg gagatatgca gcgcctgaac tcgctgtttt
     7501 gttcggtggc ttacagtgtg ctggaacagc cggatgaaga tgaaggacgg gacgagtatt
     7561 aatattgtga atcctgtagg cctgataaga cgcgtcaagc gtcgcatcag gcactatgaa
     7621 caagatgccg gatcatgcat ccggcaattc acttaaaaac ggtgaccagc ttccagcgga
     7681 accgcttctt caccagcctt aaacacataa gtggtgttcg ggccacccag cgtagcgtga
     7741 cctttactca ctgtgatcca gttaccttcc ggcagaccaa taatcgtcag ttctggcgcg
     7801 acgaccagca gttcgcgaat acgctgctca cgggtttcac ctttatggcc ttccggcagc
     7861 gcgttggtga agtgcgggtt gatttgcaac gggaacagat ttagcgcatc gaaaccttgc
     7921 ggatcgacaa tcggcatatc gttggtggta cgaatggttg ggcaagcaag gttagcgcct
     7981 gcgctccagc caatatacag cgcgccacgt ttaaccacgt cagtaattgg agccagcagc
     8041 ccgcgctcgc ggcactgttt cagcaactgg aaagtattac cgccgccgac aatcacaatt
     8101 tccgcatttt caatcgcggc aacgggatcg acaacgctat gaataccggt gacagaaaca
     8161 cccagcggag cgagaaccgc agccgttttc tctgtgtaat catcccaggt ctgcgttacg
     8221 ccagcgaaag ggataaacac cgctgagcgg cgaccctgca actgttcagc aattagcggc
     8281 agtgcatgtt ccagccaggc tttacccggc agcgtcgagt tactcaataa aagcagttcc
     8341 attacttctc cggttagtaa gaaatcacgg aaggcatgct accactgcga atttaaccac
     8401 ttactgacat ggctcacgat gtcacgtatt gcaccgggcg ctgagactga tgacaaacac
     8461 agcattgcct gatgcgctac gcttacctgg cctaccagat aactgcaata tattggattt
     8521 tcaaaattat gtagaccgaa taaggcttca ttccgcatcc ggcataaaca atgtgtactt
     8581 tgccaccaat ttgagcgccc cttaaggcgc tgatattttt tatccttctc gtgcaaattg
     8641 tgctgcttca gcaatcagcc gcacatccgg tcgcgaagtt gctggggcga ccagccagcg
     8701 acatgcagag ttaagtagcg cgattaatca tgggaaattg cctctgcatg ggcgagcagg
     8761 tattgctcca cctcaccgga ccagatcccc tgatgacgcg ccagaatcgc cgccagtgct
     8821 tgatcctgaa tctgcgtgag tgacgtcagc ggcaggtatt tcccggtata caccagcttt
     8881 ttgccgccgc cgacggcagg caattcaagc gtggtttcgc ccgcggcatt cagcccaaga
     8941 atatgtgtta ccacttttgc ggcctgcact tttttctctt cgatcagttt gaccgccgcg
     9001 cgcatgtcgt cggtattgcc gcctgacgtg cccacgtagt gggtaaatgc ataatgcaca
     9061 tcgtagaaat taattggcgc gctgaaatgt ttatcctgcg gtccggcgaa gaaattcagg
     9121 caaccatctg tcgccagcaa ggaagaggcg agagtcacca gtccttcatt aggcacaaag
     9181 acgaaaatat catcgaagcc gtgaccgcca ctcagcgcca ttaacgtatc aaatgctgca
     9241 tcggtggcat tgagataatg aatcagtgtt tgcggttctg atggatagtg cttgcgcgca
     9301 taactcaatt tgtcgttgtc ggtatcggta atgacgagca gcgacgggtt aactggtcca
     9361 tgcagcgcat aatcaatcgc caacagtccc attggtccgg tgccgccgag gatcagcgtg
     9421 tgcccttgcg ggcgaatccc catcgtgtgg ttataactac cttcctgaag atgatagttg
     9481 gcgttgaacg cgccaatcac gcaggaaagc ggttcaacca gtgagccttc aaaataggtt
     9541 tcgccgtcgt atgccagcag gcaatattgt tccatgacct cgttgggaat aaccacatgc
     9601 gtcgcttcgc cgtctaccca cgggaaggag tagccggggc aatccgggcg gtcggggagt
     9661 tgcaggttgg cctgaatcac ataacgctga cccggctgga atttgtgctg ccacttttta
     9721 cccacggcca gaatatcgcc gcaaaattcg tggccgatga tgattgggtt ggtcgtcaca
     9781 tcgtcgggta cttttttatg gttttcaccc agattggcct ctttccagga agagaggcac
     9841 aggctgtcag tgaccaccgt tgcgaggatt tcatcctcct gcatttcagg aaggtcaaag
     9901 gtttccaggc gtaaatcacg tttaccataa agacgcagag ctgttgtttt cataaaccac
     9961 ctcatgttga gcgatggcct cggcagtcgt gccggggcga agaatcagga caggaagccc
    10021 agagcattgc cgataatccc taagccaaac agggcgaaaa tcagccatac cgggttaatt
    10081 tttttgttga gcagacgaac cattagtagc gtcagaccga gcgccagcaa accagggcaa
    10141 agttggtcga gaatgttctg cacggtcatg gtgacggtgg agccatcggc ggcatgcgtt
    10201 tgtgaaacca ccaacggtac gttgattgac gtccatttgg tcaccagcac gcccatcaca
    10261 aacagtccga gaatcgacgc gccttcggtg agtttttgca gcacattccc gcccatatcg
    10321 ctgacgatat tcaccccttt gcgaaagccg agctgtaggc cataccactt catcgccaga
    10381 cgcaccgcgt tgaaaataaa gaagaacagc agcgggccga gaatgttgcc cgaaagtgcc
    10441 agagatgcgc cgagcgcggc ggtaatcggg cgcagcgttc cccagaccag tggatcgcca
    10501 actcctgcca atggtcccat cagaccgact ttgatgccgt tgatggtacc gtcatcaatt
    10561 tccgcgccga tgaccgggcc acatacggct ggcgtggtat tgaagaacac caggtgtagc
    10621 ctgagcgccg caacctgatc ctcttttaat gggtaaagtc gcttgatggc ggggatcatg
    10681 tcgtagcaaa agcccagccc gtgaatacgt tcaaagttaa aggacgcctg ttgcaggttg
    10741 gagcgcagaa acatgctcac cagatcgctg cgtgtaattt ttctctgttc catgatggct
    10801 ccgttaatcg tcaagttggt caagggcggt ggaggcggtg gcctggagtt gaggttcagc
    10861 tttacaccac tgtggattta gctggatgta gaccagggcc atgatcacgc cgacgccacc
    10921 gaacgccagc aggctgagat cgaggtagcc acctgcgagg aaaccgagga aaaagaaagg
    10981 catcaaatat ttcacgccca tcatacgtag caccatggcg taaccgacca ccacgataaa
    11041 accgccagca atctgtagtc ctcgggtcac aaattcggga atggcgttta acatattgct
    11101 gaccatatcg gcgctgacga acagtgagac aatcagtgcc ggaatggcga cgcgcagcgc
    11161 ctgcacgcca agcgcggaga catgcagaat atcgagagtg cgaaaccgcg cctcttctgc
    11221 tgctttatcc gccgcgtgct ggaacactac ggtgatggta cgggcaaaca ccgtcagcac
    11281 ctggcctgcc gcagccacag gcaacgcgat ggcgattccg gtggcgatgc tttgctgacc
    11341 aacgataacc agaatggcgg agattatgct ggcgagtgca gaatccggag attgcgccgc
    11401 gccgacgttc atccagcaga gagctatcaa ttccagcgta ccgccgagta ttattccggt
    11461 ttttaaatca ccgagaatta aaccaatcac cgtacaggca attaacgggc gatgagtctg
    11521 aaattcatcc agcacgctgc ccattccggc aatacaggaa aaaagaaata tagcaatgat
    11581 ttgtagggta cttatttcca tgatttgtca ccttttaagt gtcgtcatcc ctgcctgtta
    11641 aagcatatgt acacaggcgc tattatttaa tttgcgatta actgttcgtt tattttgtcg
    11701 ataatattga ttgagggatc tgacgctacg acgcgtaaat ccagtatgac accaagatta
    11761 tttaactcat gaaatgcgtt aatatcatca tcatctaatg aaaccgcttt ggttaactgt
    11821 ttttaccggg ccgccaggcc atgccgccaa tattcagggt gcctattttt accccctggc
    11881 gaaccatcgc taaggcatcc tgtgggcgag tgaaaagata aaaaacggtt tcgtcctgat
    11941 attgcgggtt gtgataaacc gcgacggctt tttcaatatt gaccacgtta accttcattc
    12001 ccggtggcgc agcctgacgc aataatgtcc ggcgcacttc atcgttataa acttcgtcat
    12061 tgcagataat aatgcgctgg gcgttagcca ctttcgacca gacggtagtg acctggccgt
    12121 ggatcagacg gtcatcaatg cgggcgagcg taatgttcat cagaaatcct cctctgtcgt
    12181 tgccagttgt ttccagacaa cgcaggtttg ctttgcgatt tgcgtcaggt gttcacacag
    12241 ttcatcaaca ttcatgctgt cctggttatc aaccagttcg agcgccagcg gcagagaaag
    12301 gccgctgatt acccgcaaac gaggattgcg catcgcaagc attgctgccg cattccacgg
    12361 gctgccgcac tgtaaatcca cggcaatcag ccactcatct tgattgtgaa tgcttactaa
    12421 cttttccagc tttaccgcaa tgtctccggc gttttcaccg ggcacaaatg caacggcttc
    12481 aacattggcg ttgccgtaaa ccatctgaac cgattccagc atggcgcaag ctaatttgcc
    12541 gtgggcacaa aaaatggcgt tgaccataaa gcctccttag ccgcgcgttt tgccgcccgc
    12601 aatgttagtg gttactccgg tgatgtagct ggcgcgttca gacaacagat aacaaacaaa
    12661 atcagctact tctgctaatc ttccggcgcg cccaatagga atggcgtttt tggtatagcc
    12721 ttcacgcagc tgctcgacgg tgatattgcg cgtccacgcc agcgcctctt catattccgg
    12781 cgtgcgtagc ccggtttttt ccagaatccc gggcgcgata ccgaccacac ggataccgtg
    12841 tttacccagc tctttcgacc aggagcgcgt gaagctattg agcgcggctt tggtagcggc
    12901 gtaacagctt tgtccttctg agccttccag cccactttct gaggaaacat tcacaatcac
    12961 gccatcatgt tgtttgacca tctgtcgcgc caccgcctgc gacatcagaa aaacaccttt
    13021 ctggttgata ttgaccattt tttcgaatgc agcttcgttg agttcatact gcccggcagg
    13081 cgctttctca tcgaccagca gacgcgggaa attgaccccg gcgttattga ccagaccgtc
    13141 gatgcgacca aaacgctgga taatttccgc taccgtatga tttacctctt ttgtgctaga
    13201 aatatcggtc ggccaaaact gatagccttt atgaccttca tattgaccat cgccaccgtg
    13261 aatatcgacc atctgtacat tcgcgccttg tgctaataat tcctctacaa tcgccagacc
    13321 aataccggat gcgccgccgg tgacaataat gattttatcc tgcaaattta accacgtttg
    13381 cataacagac tcctgatttt agataatagg gacccgccgt aatttttatt acggtaaaca
    13441 tcgggaagat ttttatttca gtaatagttc agctgtgctg ctattcgtta ccagacaatt
    13501 aatatatttt ccacgcagtg caccaataat tccgctgtat ttttcttcac tcatggcaat
    13561 gccaatggaa tatcgcgcct gctttaattt attcatttcg atagagagcg ttttttcgct
    13621 catattcgtt tcaaccattg cgccgtgaat atcaaaaaag cgcgagcaaa tatcgccagc
    13681 aacctggcgg gcattcaggt cgtcactctc ttcaccacca taaaacgcat gccagttagc
    13741 gccgtcgcga atggccggtg agccaattcc caccagggcg atatccagat tatcccagta
    13801 ggcagagatg gttttaaagt gctgagactg catgatccca ttacgaatta atgggttatc
    13861 cagcagagcc ggaaaatccg cgagatgcga ttcccctttc agcttcgctg ccgcgctgta
    13921 ggttaatgtg tttacatgat agcggctttc gagtttaccg gacggtccgc caataattgg
    13981 tacgcagatt aactgccgcg attgccccgc ctgcggcaag ttttcaacca gtgcgctcac
    14041 cgcgcgaccc caggaaaaac cgacaatatc gccaggctcc agcaagcgat ccagcagttg
    14101 cgcgccgtgt aaccccatca tcgccagttg agtctcttca tcctcatcat ttcccgacac
    14161 caccacaacg tctttcaggc caaacttttg cttcagttgc tgctccagcc agagattttc
    14221 gttgtagtca tagttgatgg cgatggtgac cattccctga tcgcggcctc gtttaagcaa
    14281 gcggctgatg gtggtgcggt aaatacccag ttcgcgcgcg atttgcgcct gcgtcatatc
    14341 ctgttcgtaa tagagttggg caatcttcac aatcaaacgg atatcgtcac tgttttccat
    14401 tgccgcctcc tggttctgca catttgtgca aatacgcttt ctcaccgctt gatctgatat
    14461 aaccccatca tgagggcctt tcaaagcgat ttttcactct ttgcatgggg agaaatagcg
    14521 ctttctcttg cacatttgtt tctctgcgta gtagtcatca gcacaggtgt gcagcgattc
    14581 gtaatatgtt gattatcctt attttgccag gcaaatttgc atcgtacttc cggagacact
    14641 tttgtgataa aaggcgtggc agatcacatt taatttcgac gttgccgcat aaccctgttt
    14701 ttccccgcta agaggtacgg tatattccgc gttttacagg agaatttatg ctgcccgact
    14761 catcagtccg tttaaataaa tacatcagcg aaagcggaat ttgctcacgc cgcgaagcgg
    14821 atcgctatat cgagcaaggc aatgtgttcc ttaatggcaa gcgagccacc attggcgatc
    14881 aggtgaaacc cggcgacatt gtgaaagtaa acggtcagtt gattgagcct cgggaagccg
    14941 aagatttggt acttatcgcc ctgaacaagc ccgtcggtat tgtcagcacc accgaagatg
    15001 gcgagcgcga taacattgtc gatttcgtta accacagcaa acgcgtgttc ccgattggcc
    15061 gcctggataa agactcccag gggctgattt tcctcaccaa tcacggcgat ctggtgaata
    15121 agatcctccg tgcgggtaac gatcatgaga aagagtatct ggtgacggtc gataaaccga
    15181 ttaccgatga atttattcgt ggtatggggg cgggggtgcc gatcctcggg acggtgacga
    15241 aaaagtgcaa agtcaaaaaa gaagcgccgt ttgtcttccg cattacccta gtgcaggggc
    15301 tgaaccgtca gatccggcgc atgtgcgagc atttcggcta tgaagtgaaa aagctggaac
    15361 gtacgcgcat tatgaacgtc agcttaagcg gcattccgct gggggagtgg cgcgatttaa
    15421 ccgacgatga gttaatcgac ctctttaagc tcatcgaaaa ttcctcttcc gaggcaaaac
    15481 ctaaagcgaa ggccaaaccg aaaacagcgg gcatcaaacg tccagtcgtt aagatggaaa
    15541 aaacggcgga aaaaggcggt cgcccggcgt ccaacggtaa gcgttttacc tcgccggggc
    15601 gtaaaaagaa gggacgctga ttaacgcctc agactgatga aaacgtccaa aaagagctcg
    15661 gacagtcccc tcgccccttc ggggagaggg ttagggtgag gggaagattg tgtccacaca
    15721 gacgagtgaa aatctcaggc gttgattgtt cattagcgcc tgccgcgcgt cgacgtattt
    15781 gctgaccagc taaatgacgc ttcaggatcc ggtttataag cctgcttttt cttcaattgg
    15841 cgggcttttt tcgcttcagc ctcacgctcg accattaatt tatcgatgta ctctttctta
    15901 atgctgttgg tttcggagtt ggtcagtacg
//
LOCUS       DADLTY010000093        15833 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.093, whole genome shotgun sequence.
ACCESSION   DADLTY010000093 DADLTY010000000
VERSION     DADLTY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.093"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..881)
                     /gene="adiC"
                     /locus_tag="JN334_002529"
     CDS             complement(<1..881)
                     /gene="adiC"
                     /locus_tag="JN334_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HAY7438631.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWT"
     gene            complement(1018..1779)
                     /locus_tag="JN334_002530"
     CDS             complement(1018..1779)
                     /locus_tag="JN334_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7438632.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(2104..4371)
                     /gene="adiA"
                     /locus_tag="JN334_002531"
     CDS             complement(2104..4371)
                     /gene="adiA"
                     /locus_tag="JN334_002531"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAY7438633.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(4570..5478)
                     /gene="melR"
                     /locus_tag="JN334_002532"
     CDS             complement(4570..5478)
                     /gene="melR"
                     /locus_tag="JN334_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HAY7438634.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            5761..7116
                     /gene="melA"
                     /locus_tag="JN334_002533"
     CDS             5761..7116
                     /gene="melA"
                     /locus_tag="JN334_002533"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAY7438635.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            7231..8640
                     /gene="melB"
                     /locus_tag="JN334_002534"
     CDS             7231..8640
                     /gene="melB"
                     /locus_tag="JN334_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAY7438636.1"
                     /translation="MTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLF
                     LVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEGTTQI
                     VFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTL
                     PFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHLTLKA
                     IVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANL
                     VTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGT
                     ALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGY
                     VPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKV
                     PPEPVHADIPVGAVSDVKA"
     gene            complement(8779..9408)
                     /locus_tag="JN334_002535"
     CDS             complement(8779..9408)
                     /locus_tag="JN334_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HAY7438637.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(9531..11177)
                     /locus_tag="JN334_002536"
     CDS             complement(9531..11177)
                     /locus_tag="JN334_002536"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAY7438638.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(11255..12595)
                     /locus_tag="JN334_002537"
     CDS             complement(11255..12595)
                     /locus_tag="JN334_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY7438639.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(13166..13885)
                     /gene="dcuR"
                     /locus_tag="JN334_002538"
     CDS             complement(13166..13885)
                     /gene="dcuR"
                     /locus_tag="JN334_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HAY7438640.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(13882..15513)
                     /gene="dcuS"
                     /locus_tag="JN334_002539"
     CDS             complement(13882..15513)
                     /gene="dcuS"
                     /locus_tag="JN334_002539"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HAY7438641.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            15694..>15833
                     /gene="yjdI"
                     /locus_tag="JN334_002540"
     CDS             15694..>15833
                     /gene="yjdI"
                     /locus_tag="JN334_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAY7438642.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
BASE COUNT         3869 a         3818 c         4051 g         4095 t
ORIGIN      
        1 gtccagccgc ccagtgaacc taagcaaccc gcagctgcgc agaaggaaac aatggcccca
       61 gcggtgtcac ccaacgccat ccgtgcggca tcaccgaatg gcgaggcaga aacgcgcagt
      121 gcggcattag ggatcatccc cataatcgcg gtggtggaga gcacatagca aacggcggca
      181 atcaataccc caccaatggt ggcgataggg acattgcgtt tcgggttttt caccacacct
      241 gcggcaacgg aggcactttc cacaccgatg aacgaccaca gcgtaacgtt aagggtactt
      301 tgaatcgcac cgaaggtgcc caggccgctg acgttccatg ccgccatata ggtttcacca
      361 cggaaccaga accagccaaa tacggcaatc ccgacgatgg ggatcagcgc cagcacagtg
      421 gcaacggcct gcacgcgggt gatcattttc ggaccgacaa tgttcagcag gacgaagatc
      481 cacagcacca cgacgcaggt gatggttaat accaatggat ctttcagaat cgggaagaag
      541 taacttaaat atcctacgcc aatgaccacc atggcgatat tgccgatcca gcaggccagc
      601 cagtagagga cgttggtttg ataaccgaga aacgggccaa agcagcggcg ggcgtaagcg
      661 taagaaccac caggacttgg gtcgaggaac gacattttgg cgtataccat cgagagcccc
      721 agcgcaccga taatcgtcac caaccatcca taaatggcaa tcccgccagt ggaggccagg
      781 tttgcaggta acagaaaaac acctgacccc ataatattcc ccgacaccat cagggtgacg
      841 gggattaagc ccactttgtg agcatcagca tccgaagaca taattaaact cctgcgaagg
      901 cgagcttcgt gacaataaat tacgtcatat catacgcctg cattcgatag ttgaattatt
      961 atcgaccggg ttatcgctga tgccgttatt aatatagctc ttcgttaagg ttaaaaatca
     1021 ggcgacagtg cgttctctgt gctgactaac ataatgcagc ggcgtcatac cgtagaagtc
     1081 tttaaagaca gaaataaagt acgacgtact gttgtagccg caggactgtg atacctgaga
     1141 gatattttta ccgtccatca ttaattcatt tacggcataa cgcatgcggc aggtggtgat
     1201 tatttggcta taactggtgt tttcgctttt caactttttc tttaacagac ttgggctaag
     1261 acataaacaa ctggctacca tactaagatt ccagtctttg tgaatatcgc tttcaataat
     1321 ttgataaacg ctgtcactta cacaattacg taacatatac atcattaatg aaaggaattt
     1381 tttactgtcg agaaaacggg atagcaccgt gaataataat gcgcgtgttc gttccttttc
     1441 tgactcagtt tctgcgggca tcatgctgtg ttgcgcggca acgcgaaaga cgtctggcgt
     1501 caggcatggc agggtgagta tgggcgtagc gctacgttgc catactggta tttgcgagag
     1561 atctttattc agaaaccgga ggtaatcttt gatgatgtcg tgactaatat gagcaaccaa
     1621 agtgttgtta agagaggaga cgtcgataat attattttca caatttaata acgccatatg
     1681 gttagcttta aggctaatag gttctttccc attcaccctt atccagacat ctttttcagt
     1741 caataaaaca atacaaggtt ggtcgctgca aatcctcata catgtactcc tgagtgcgaa
     1801 taaaagacca gttctcgcta aagcaaagcg ataccgggaa atggttatat aattgcatga
     1861 tgaatataaa catgcataaa aataacgagt tttactaata ttacaaattt ttatagtaat
     1921 ttctcatgta catatttttt tacaggataa ataacaggca aaaaaaagcg tggggtgggg
     1981 ccgcgcaaaa agtatgacat ttttgtttgc agcaattttt atgaaaacgt tgccatactc
     2041 aaaaggccta aggccaggcg gcgtaatgtt atttaaacaa ttacgccttc agcggaatag
     2101 tggttacgct ttcacgcaca taacgtggta aataccgtca ataatttcag tcccttcagt
     2161 ttcgtgttca aatctaggga aatggtggtc ccaggattgc agcgagcgta aataacttac
     2221 ttgcggactg tttttatcgc cgaagttttc accagacagc agcatcggga ttcctggcgg
     2281 atacgggata actgagtttg ccgcgatgcg tcctggcaga ttttcaatgg ataccagttc
     2341 gacattgttg tcgacaatcg cgttgtacgc ttcacgcggg gtgatttccg ccaccggcag
     2401 gccggaatag gcttcgttca accgtgcgcc agggttgttt tctttcagcc aggcaaacat
     2461 ggtgtcaccc agatcgtgaa tccccatgtt cgcgtaagtg tcaggatatt gttcaacaag
     2521 ttccggcatc acctgcgcca gcggtgtgtt ggcgtcatag tggtgtttga aggagcaaag
     2581 ggtgttaacc agagtccccc atttcccacg ggttacgccc atagagaaca ggaacataat
     2641 ttggaagtca gtggtgcggg taggcacaat gccgtggcga ccaagccagg cagtgaccag
     2701 cgccgccgga acaccggttt cttccagttc accatcttca cccattcccg gagcaaggat
     2761 gctgacttta attgggtcga gcatactcca gttatccgga atatctttga agccgtgcca
     2821 gctttcgccc ggatgcatta cccagcagtc ctgaacggtg gtcagcagtt tggttggtgc
     2881 gtcagcaaag tcataggttt tgccggtttg tgggtcggtg acgacttctt tgttccacgg
     2941 tttgaagaac cagctaccgt cagcggtgaa ctctttatat agccgcgcca tcgcctgacg
     3001 gaaatcaacc gcttcgtcaa tcacttcctg tgtcagtgac aggccgctgt tgccgtccat
     3061 catcgacacc gccacgtcgt tggatgcgca gatggcatac agcggggagg tggtggcatg
     3121 catcatgtag gcctggttga agcgggagaa gttaatcgcc ccacgacctt cacgtacatg
     3181 aatataagaa gcctgtgaca gcgcattcag cagtttgtgg atggagtggg tggcgaaaac
     3241 ggtaggaccg ttgtgatcgc caggttcgcc gcgcatggca tagtgatcgg catagatcgg
     3301 gttgaaacgt gcatagccgt accaggcttc gtcaaagtgc agacgatcgg aggttttttc
     3361 cagcagatcc tgcgcttctt tagcgttata acacacgccg tcataggtgc agttggttac
     3421 cacgcagtaa gacggttttt gcccggcttt gtctttggtc agcgggcttt cactgatttt
     3481 cttttgcaag gtttcaggtt gcatttcctg cggatagatt ggcccgataa tgccgtagcg
     3541 gttgcggctt ggcaccatat agaccggttt cgcgcctgtc agcatcaaac cttgttcgat
     3601 ggatttatgg cagttacggt caacgaccac gacatcgtta tcggtcatgc aagcctgcat
     3661 gatggtgcgg ttagagccgg aagtaccgac gactaccgac caggagcgat cggcaccaaa
     3721 tacgcgtgcg gcatattttt cgctttcgcc aaatgcgcca gtatggtcaa gcaaagaacc
     3781 gagggaagtt cgttcgatgc ccatgtcggt acggaacaga ttttcaccat agtagtcatg
     3841 gtagaaacgt ccggcgggtg ttttggtaaa accaacgccg ccctggtggc ctggcgctgc
     3901 ccaggaatat tcatggatgt cactatattt catcagcgcg ctgaacagtg gcggcaacag
     3961 ctgctggcgg tagcgggtca tcgcggcaac ggcgcgtccg gcgataaagt cggcggtatc
     4021 ttccagaatc caggcgaatt catcgacaag ctccagcaga tcccggtcca ttgcggcgag
     4081 ggctttttcc cgatcgccca acaggaagac cggcacgttt tgttggcgct catgaagctt
     4141 accgatcaat tgtctgacgt tttgatgttc gtccggatgc tccatttgat agctgaacat
     4201 caggcagtca atggcttcgt ttgaagagag aatggcaaaa ccatcatcaa aggaggtgga
     4261 tttaatcacg gtaacatttt gctggcttaa agcatctgcc agacgctcaa cggcgttacc
     4321 gacccaggtg tcttgatgga gaaactcgct ttcaacaatt aatactttca tcattgctta
     4381 cccggttatg aaggaatctt cgttgcgggc aagtatcttc cggccttttc gggctgtaaa
     4441 gcgcgtgaaa aacaaacatt tttgaaatat ttagttttat aaacaatacg ttatgtgatt
     4501 attttaaatt attttcactg cttattatcg acggctaaac tatttttttg gctgatactg
     4561 atatcgtctt tagccgggaa acgtctggcg gcgctgttgg ctaagtttgc ggtattgttg
     4621 cggcgacatg ccgacatatt tgccgaacgt gctgtaaaaa cgactacttg aacgaaagcc
     4681 tgccgtcagg gcaatatcga gaatactttt atcggtatcg ctcagtaacg cgcgaacgtg
     4741 gttgatgcgc atcgcggtaa tgtactgttt catcgtcaat tgcatgaccc gctggaatat
     4801 ccccattgca tagttggcgt taagtttgac gtgctcagcc acatcgttga tggtcagcgc
     4861 ctgatcatag ttttcggcaa taaagcccag catctggcta acataaaatt gcgcatggcg
     4921 cgagacgctg tttttgtgtg tgcgcgaggt tttattgacc agaatcggtt cccagctaga
     4981 gaggctaaat cgcttgagca tcaggccaat ttcatcaatg gcgagctggc gaatttgctc
     5041 attcggactg tttaattcct gctgccagcg gcgtacttca aacgggctaa gttgctgtgt
     5101 ggcaagtgat ttgatcacca tgccgtgagt gacgtggtta atcaggtctt tatccagcgg
     5161 ccaggagaga aacagatgca tcggcagatt aaaaatcgcc atgctctgac aggttccggt
     5221 atctgttagt tggtgcggtg tacaggccca gaacagcgtg atatgaccct gattgatatt
     5281 cactttttca ttgttgatca ggtattccac atcgccatcg aaaggcacat tcacttcgac
     5341 ctgaccatgc cagtggctgg tgggcatgat atgcggtgcg cgaaactcaa tctccatccg
     5401 ctggtattcc gaatacagcg acagcgggct gcgggtctgt ttttcgtcgc tgctgcacat
     5461 aaacgtatct gtattcatgg atggctctct ttcctagaat atcagaatta tggcaggagt
     5521 gagggaggat gactgcgagt gggagcacgg ttttcaccct cttcccagag gggcgagggg
     5581 actgtccgag catcatgagg ccgaaaactc tacttttcag gtaatttatt cccataaact
     5641 cagatttact gctgcttcac gcaggatctg agtttatggg aatgctcaac ctggaagccg
     5701 gaggttttct gcagattcgc ctgccatgat gaagttattc aagcaagcca ggagatctgc
     5761 atgatgtctg cacccaaaat tacatttatc ggcgctggtt cgacgatttt cgttaaaaat
     5821 attcttggtg atgtgttcca tcgcgaggcg ctgaaaacgg cgcatattgc cctgatggac
     5881 attgacccca cccgcctgga agagtcgcat attgtggtgc gtaagctgat ggattcagca
     5941 ggggccagcg gcaaaatcac ctgccacacc caacagaaag aagccttaga ggatgccgat
     6001 tttgtcgtgg tggcatttca gattggcggt tatgaacctt gcacggtgac tgatttcgag
     6061 gtctgtaagc ggcatggtct ggaacaaacc attgccgata cgttggggcc gggcggtatt
     6121 atgcgcgcgc tacgtaccat tccgcatctg tggcaaattt gcgagaacat gacggaagtc
     6181 tgccccgatg ccaccatgct caactatgtt aacccaatgg cgatgaatac ctgggcgatg
     6241 tatgcccgct atccgtatat caaacaggtc gggctgtgcc attcggtgca gggaacggcg
     6301 gaagagctgg cgcgtgacct caatatcgac ccggctacgc tgcgttaccg ttgcgcaggt
     6361 atcaaccata tggcgtttta cctggagctg gagcgcaaaa ccgccgacgg cagttacgtg
     6421 aatctctacc cggaactgct ggcggcttat gaagcagggc aggcaccgaa gccaaatatt
     6481 catggtaata ctcgctgcca gaatattgtg cgctacgaaa tgttcaaaaa gctgggctac
     6541 ttcgtcacgg aatcatcaga acattttgcc gaatatacgc cgtggtttat taagctaggt
     6601 cgtgaggatt tgattgagcg ttataaagta ccgctggatg agtacccgaa acgctgcgtc
     6661 gagcagctgg cgaactggca taaagagctg gaggagtata aaaacgcctc ccggattgat
     6721 attaaaccgt cacgggaata tgccagcaca atcatgaacg ctatctggac tggcgagccg
     6781 agtgtgattt acggcaacgt ccgtaacgat ggtttgattg ataacctgcc acaaggatgt
     6841 tgcgtggaag tagcctgtct ggttgatgct aatggcattc agccgaccaa agtcggtacg
     6901 ctaccttcgc atctggccgc cctgatgcaa accaacatca acgtacagac gctgctgacc
     6961 gaagctattc ttacggaaaa tcgcgaccgt gtttaccacg ccgcgatgat ggacccgcat
     7021 actgccgccg tgctgggcat tgacgaaata tatgctcttg ttgacgacct gattgccgcc
     7081 cacggcgact ggctgccaag ctggttgcac cgttaaaacg cgactaaacg ctactgcgcc
     7141 gggggattta ttccggcgca cacctctgac gataccaata acagaaggcg ggcgttggta
     7201 acagcgaccc gataccctat gagcatttca atgactacaa aactcagtta tggatttgga
     7261 gcgttcggga aggattttgc gatcggcatt gtgtatatgt acctcatgta ttactacacc
     7321 gatgtcgtcg ggctgtctgt gggtttggtc ggtactttgt ttctggtggc gaggatctgg
     7381 gatgctatta acgatccgat tatgggatgg attgtaaatg ctacgcgatc gcgatggggt
     7441 aagttcaaac cctggatcct gatcggtacg ttggcaaact ctgtaatctt atttctcctc
     7501 tttagtgcgc atctgtttga aggtactact cagattgtct ttgtttgcgt gacctacatc
     7561 ctctggggca tgacttacac cattatggat attcccttct ggtcgctggt tccaaccatc
     7621 acgctcgata aacgtgaacg cgaacaactg gttccttatc cgcgtttttt tgccagtctg
     7681 gcaggctttg ttacggcagg tgtgacgcta ccatttgtta attatgtcgg cggtggcgat
     7741 cggggatttg gctttcagat gttcactctg gtattgatcg ccttttttat tgtttcaacc
     7801 atcatcactc tgcgcaatgt gcatgaagtc ttttcgtcag acaatcaatc gtctgctgaa
     7861 ggaagccatc tgacacttaa agccatcgtt gcgcttattt ataaaaacga tcagctttca
     7921 tgcctcttgg gtatggctct tgcttataat gtagccagca acattattac cggatttgct
     7981 atctattatt tctcatatgt tatcggtgat gcggatttgt tcccctatta tctgtcgtat
     8041 gcgggagctg ctaacctggt gacgttagta ttcttcccac gcttagttaa atcattatcc
     8101 cgacgcattt tatgggccgg agcatctatt cttccggtgt taagctgtgg tgttctcctg
     8161 ttaatggcat taatgagcta tcacaacgtc gtcctcattg tgattgcggg tattttgctg
     8221 aatgtgggaa cggcgctttt ctgggtatta caggtcatca tggtggcaga taccgttgat
     8281 tacggtgaat ataaactgca cgtacgctgt gaaagcatcg cttactccgt gcagactatg
     8341 gtggtgaagg gcggttcagc ctttgcggct tttttcattg cggttgtgtt agggatgatt
     8401 ggctatgtac cgaatgttga acagtctacg caagccctat taggtatgca gtttattatg
     8461 attgctctac cgactctgtt tttcatggta acgctgattc tctacttccg tttctatcgc
     8521 ctcaatggcg acacgctgcg caggatccag attcatctgc tggataaata tcgcaaagta
     8581 ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag tgagtgatgt gaaagcctga
     8641 cgtggacaat agctaacgaa aatggcctga tgcgatatgt ttatcaggcc acgtcttaat
     8701 gtaatgcttt ggatttgcaa attttgtaga ccggataagg aattcacgcc gcatccggca
     8761 tcaacaaagt gcacgttgtt atccagttat caagccatag cgccgtagct ggcggcaatg
     8821 aaagcgagcg agcaatatca ctgtcgttaa tgcgccaagc agcgcgcaga acatatcgga
     8881 ttgcgtgtcc cactggtcgc cctgggtacc gagaaaatca tccgctccct ggcccatcgc
     8941 cagtgccgcc caccactcga ttaattcata catcgcgctt atcgccagcg ccacgcaaca
     9001 gaccagaaac gccaccattt tacgtccgcg cacgtacatc ccgcgaacga gaatttctcg
     9061 tgccaccagt gcaggcacca gcccctggaa aaagtgcccc agcttgtcat acggattacg
     9121 actcaacccc agccattcct gcacctcaaa accaacgggg actttcgcgt aggtgtactg
     9181 cccgcctacc atcaggatga tggcgtgaaa gaagatgagc gtatagagca gcagcgttaa
     9241 cgggtaacgt ctggcggtgg caagtagtag cggcacgacg ataatcaccg gtgtcacttc
     9301 cagcagccag gtgagtttgt catgggccga aatgccggta taaatcagga ttaacgttag
     9361 cgccagtgcg ccagtgttaa gaattaacgg cttgagtgtg cgggtcatgg tgagttcgca
     9421 agtcagggaa aatactgact attcaccagc aagcatgaaa ttacaatcgc aaaagaggtg
     9481 gcccaggggg atcacctggc agcatgctgc caggcgctgg gccgaagagg ttacttagtg
     9541 cagttcgcgc actgtttgtt gacgatttgc tggaagaagt cgttaccttt gtcatcgacc
     9601 aggataaacg ccgggaaatc ttctacttcg attttccaga tagcttccat acccagctcc
     9661 ggataagcga cgcattccag atgcttgatg ctctgctgcg ccagtaccgc cgccggaccg
     9721 ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg cgtcggtaac ctgctggctg
     9781 cggttgcctt tcgccagcat gatcatgctg ccgccgtggg attgcagcag atccacatag
     9841 gagtccatac gacctgcggt ggttgggcca agtgaacctg atggataacc ggcaggggtt
     9901 ttcgccggac ccgcgtagta gatcgggtga tctttgatgt actgcggaag ttctttaccg
     9961 gcgtcaatca gctctttcag cttggcgtgt gcaatatctc gacccacaat aatggtgcca
    10021 gtgagcgaca aacgagtgga aaccggatat tgcgaaagct gggcgaggat ctctttcatc
    10081 gggcggttaa ggtcgacctt caccgcttcg ccttcaccgg cctgacgcag ttcttgtgga
    10141 atgtactgac ctagattgtg ttccagtttt tcgatccaga taccttcgcg gttgattttc
    10201 gctttaatgt tacggtcagc ggagcaggag acgcccatgc ctaccgggca ggatgcgccg
    10261 tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt atttaccgcc aaactgcgcg
    10321 ccaagaccga gtttctgggc ctcttccagc agttcctgtt ccagctggac atcgcggaac
    10381 gcctgaccat gttcgttccc ttccgtcggc agttcatcgt aatagtgagc gcttgctaat
    10441 ttgacggttt tcaggttgtt ttccgcagac gtaccgccaa tcacaaacgc gatatggtac
    10501 ggcgggcagg ctgcagtacc gagggtacgc attttctcga cgaggaagtt tttcagtttg
    10561 ccgggagtca gcagggcttt ggtttcctgg tagagatacg ttttgttggc agagccgccg
    10621 cctttcgcaa cgcaaaggaa tttgtactca tcgccatcta ccgcgtacag gtcgatttgc
    10681 gcaggcaggt tagtgccggt gttgacctct ttgtacatgt ccagcgccgc gttttgcgag
    10741 tagcgcaggt tatcttcgat ataggtgtta tagacgcctt tcgacagcgc ttcttcatca
    10801 ccgccaccgg tccacacgcg ctggcctttt ttaccgacga tgatcgcggt gccggtatcc
    10861 tggcaggtcg gcagcacgcc tttggcggcg atttcggagt ttcttaagaa ttgcagtgcc
    10921 acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa tagccgcaac ctgtttctgg
    10981 tgtgccggac gtagcatgaa agaagcgtcg tgaaaggctt gctgcgccag cagggtcagg
    11041 gcttctggtt ccactttcag gatggtttcg ccgtcgaagt cggcaacgct aacgtaatcg
    11101 gaagtgagta gatagtattc ggtattgtct ttccccatcg ggaaaggtgc ctggtagata
    11161 aagggtttgt ttgacatagc ttccagcctg taactctgta tttgttattc gaaaatggcg
    11221 tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa gaacccgtac atcgcggcga
    11281 agatccagcc gaagacgcac gatacgctca caccaatcaa ccccggcaga ataaaactgt
    11341 ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg gtcaaactga atcgctgcca
    11401 gatcgctcgg ataagtcggc aggatgtaat aaccgtagca agccggtgct gaagccacga
    11461 tgtatgccgg atcaacgccg atcgccagcg cgaccggaac aatcgccgcc agcgccgcag
    11521 cctgagagtt tacaaacttg gaaaccagca gcagaacaat ggcatacgcc cacggatact
    11581 ctttcaccat ttcacccagt acgccctgaa tttcagacat atgcgcaccg aacatggttt
    11641 ctgccatcca tgcgataccg tacaccgcca cgatggcgat cataccggaa cggaagactt
    11701 cgttttttga gatagacgcg ggattggttt tggtcaggat aataatcagc gccccggtca
    11761 gcagcataaa catctgaata accagtacca tcgacagcgg tttgccgccg aaggatggac
    11821 gcaggtccga atcagcacca agaagggcga ctacagcgat tgccccgagg aaaatccaca
    11881 ttgccagcca gttgcttttc ggcagttttt tatccagcag cgtcgcggta tcaccgtaaa
    11941 catactcacg gttttccggt acggagatga atttctggaa ctcttcgtct ttatccagat
    12001 ctttaccgcg gaaccagctg aagataccga tcgccaggat accgattaac gtcgatggaa
    12061 tggtgattgc cagcagatcg aggaactcaa gatggcgacc atcaaaggtg acattaccca
    12121 gcatcgcaac cagagacacg accgcaaccg acaccggact ggcgataatc cccatctgtg
    12181 caccgataga acttgccgcc atcggacgtt ccggacggat gttgttctta atggcgacgt
    12241 cgtagatgat cggcagaatg gtgtaaacca catgacccgt accgcaaaga atggtcagtg
    12301 tacaggtcac aaacggcgcg acaattgaga catatttcgg gttgcggcgc agcagcttct
    12361 cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg caaggtcgcc gatgccgcca
    12421 ccaccgcaat gataaccagc atgacatcaa ctggtggttt acctggctga aggtggaaga
    12481 cgaagaccag aatgaccaga ccgataccgc ctaataaacc cagcgcgata ccaccctttc
    12541 tggcaccata aaacagacat attagtatta tgataagttg gatagtaaat aacatgtgtg
    12601 aaccctcgcg ataatcctat ttaaattttt gctgaataga tcacagtcac gttctgtttt
    12661 gtatgaactg ttttcagagc ctgactaaat atccgtttgg tcgttaccgg ctttagcaaa
    12721 tacctcacag tgaatattgg ctgattaaag cagcggcaaa tttatgcact gtgataagcg
    12781 gctttttcag agagaaaaac tctctggcga tgggtttata gtttgtactg cgcagatgtt
    12841 aatgttttgt ttgctgttat cttattgata tttatcggtc tattttttgt tctcagggca
    12901 gatatcatta ggtttataga ttaatctgat ctacccattt gtgggtaaaa atacacataa
    12961 tgcgggtgac ataatagtta attaactttt gttatcgttt tgaaattaaa aacaccgttc
    13021 atctgaagag atattaattt ttaacgatga tggagggata attatatttg atctggcaca
    13081 agttttactg attaacgatg taacttgtgc caggggtatt ttgcattacg gtaataatta
    13141 ttacttacag ataaccagcg atccgttatt ggcaatattg tttcagcagt gagtagtgtt
    13201 ctgcctgaat acggtaacgg taaaccggac gccccgtgac gccataatgg atactggtga
    13261 acaagatgtg gcagttgacc agccagatga ggtattttcg gcaggaaaca cgcgagatgt
    13321 taacctcgtt agccagctcg tcggttgaaa actcatagtc ctgatgcgcg tcaatccact
    13381 ggcacagcgt gcgtaacgtc tgcggcgtta agccttttgg caagcgacga ggatcctgtt
    13441 cgttggagct gctgccgtgg attagctgat caagctcggc ctggtcataa tactgatgtt
    13501 tttccagcgc cattttcttt tgccgccagc cggtgagcgc ctcttcaaag cgggaagcct
    13561 ggaagggttt gatcaggtaa tccacgacac cgtaatgcag cgaatcttta atggttgccg
    13621 catcggctgc ggaggagatg acaatcacat cacttttgca acgcgcgtta tgcaggacag
    13681 gcagtaaatc gagcccgttc tctttttgca tatagatatc gagcaatatc aggtcgatag
    13741 gcgcatcgct attgaagata atctctttgg ctttctccag cgtcgaggct gttccacagc
    13801 attgaaagcc tgggatttgt gctacgtatc ggcgattcag ctccgcgacc attgcgtcgt
    13861 catcgataat taatacattg atcatctgtt cgacctctcc ccgtcccagg gtatctggac
    13921 aaaaaattgt gtgaaaatcc ccggttccga ttccacggcg atgctgccgc cgagattttc
    13981 tacctgttgt ttgacaagtg ctaaaccgac gcctcgctcg cttccttttg tcgagacacc
    14041 tttgtcaaaa atgtgatcga ttttgtcggg tgcgatcccc ggtccatcat cgttaacttc
    14101 gcagtgcagc cagccgtgac ggtagtgcaa tgttacgcta atttcgccac cgggttccgg
    14161 ccctaatgct tccagcgcgt tttctatcag atttcccaat gtggtaatca gcgtcgcgac
    14221 ctggtcctca ctgccgctgt ctggcagctg gctttcactg tttaaaatca gcgtatggcc
    14281 taaatcggtc gcgcggttaa tcttgctgat taaaaaacca gcgataaccg gagatttgat
    14341 cttacccagc agagagccaa tctcttcctg atagttattg gctgttttga gaatgtaatc
    14401 ttccaactgc ttataactct tcagatgcaa taatccgaga atcacatgca atttattcat
    14461 aaattcgtgg gatcgttcac gaagtgcgtc agcatagttg accagaccgt cgagtcgctg
    14521 catcagttta cgaacttcag ttttgtccct gaaggttgaa atggcaccga tgataacgcc
    14581 attactgcgc accggaacgg tgttgatcag taatagccgg tctttaatcg taatctcttc
    14641 gtcgcggcgc ggcgtaccgt cgcgtaacac ttccgagaca tctaccacct gtgaccatga
    14701 gtggctgagc gtcgacagtt tctcatcgtc ctgcgactta cggtaattca gcaattcttg
    14761 tgcggcatcg ttgatcagcg tgacctcgcc gcgatcgtcc acggcaacga cgccttcttt
    14821 gatagactgc aacatggcct ggcgttgctc aaacagcgtg gagatttcgt agggttccag
    14881 gccgaaaagg atttttttca gtaccttaac cagaatgcag gtgccaatca gtccgaccag
    14941 catgccaaat aataccgacc agataatgct ccagcgactg tcattgatct gttgggtcac
    15001 gcggcttaac tcaaggccga tcgccaccac gccaatttgt ttatgatttt catcgtagat
    15061 gggggtaaat acgcgtaaag cctgcgccag aaaaccgcga ttgatagcga cattttcttc
    15121 gccattcagc gcattaagga tgtcatcacc tttaaatggc tgaccaatac gctgggcttc
    15181 aggatgtgag tagcgaagac tttgcatatc ggtaacgaca ataaacagca gatcgttgcg
    15241 tttgcgcacg gcttccgcga tggcctggat gccactctcc tgcggttttt tctgcaagcc
    15301 ctgacggatt tccggcgagt cggcgagggt acgcgccact gccagtgcct tgttggccag
    15361 cccgtctcgc gtcatatcac tgatttgcga gaagtaaatc agatgcacca ccaatagcac
    15421 cgagaacagt accgcgctga ccattaagat cacagtggta ctcaatttca tcggacgttt
    15481 gcgtaatatg tggtagggca atgaatgtct catcagcttc cttgtgtgac aaatttctta
    15541 agcattatct ctgatgaggc gggtaattca aagggagtaa aaatgattgg ctatataggg
    15601 gaagagactc tggcaacgga aactgccagt gctgtatgaa gattccgggg ctatgcttat
    15661 agcgataatc atactgatga gagagggaag gtcatggatc aggcgctact ggacgggggt
    15721 tatcgctgtt ataccggcga aaagatcgat gtctatttca acactgcgat atgtcagcat
    15781 tctggcaatt gcgtacgtgg caacggcaag ttatttaatc tcaaacgaaa gtc
//
LOCUS       DADLTY010000094        15793 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.094, whole genome shotgun sequence.
ACCESSION   DADLTY010000094 DADLTY010000000
VERSION     DADLTY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.094"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(41..772)
                     /gene="rlmB"
                     /locus_tag="JN334_002541"
     CDS             complement(41..772)
                     /gene="rlmB"
                     /locus_tag="JN334_002541"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAY7438643.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(952..3393)
                     /gene="rnr"
                     /locus_tag="JN334_002542"
     CDS             complement(952..3393)
                     /gene="rnr"
                     /locus_tag="JN334_002542"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAY7438644.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="JN334_002543"
     CDS             complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="JN334_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HAY7438645.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4062..5360)
                     /gene="purA"
                     /locus_tag="JN334_002544"
     CDS             complement(4062..5360)
                     /gene="purA"
                     /locus_tag="JN334_002544"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAY7438646.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5464..5661)
                     /locus_tag="JN334_002545"
     CDS             complement(5464..5661)
                     /locus_tag="JN334_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HAY7438647.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="JN334_002546"
     CDS             complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="JN334_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAY7438648.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="JN334_002547"
     CDS             complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="JN334_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAY7438649.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="JN334_002548"
     CDS             complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="JN334_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAY7438650.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="JN334_002549"
     CDS             complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="JN334_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAY7438651.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="JN334_002550"
     CDS             complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="JN334_002550"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAY7438652.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="JN334_002551"
     CDS             complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="JN334_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAY7438653.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="JN334_002552"
     CDS             complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="JN334_002552"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HAY7438654.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="JN334_002553"
     CDS             complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="JN334_002553"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAY7438655.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14434..>15793)
                     /gene="nnr"
                     /locus_tag="JN334_002554"
     CDS             complement(14434..>15793)
                     /gene="nnr"
                     /locus_tag="JN334_002554"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY7438656.1"
                     /translation="LVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLPEEAAL
                     AREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSHPAPIV
                     AVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLGLDSWL
                     AGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEAALRAG
                     AGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQEWGKKA
                     LQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIESDRLHC
                     AKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGALLGQK
                     LSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHDESSNS
                     AP"
BASE COUNT         3533 a         4565 c         3955 g         3740 t
ORIGIN      
        1 aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc tgacgaaccg
       61 cttcaaataa gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca
      121 ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt
      181 ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg
      241 cctcgcctgc cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg
      301 caaggttagt cacccgaatc agcggaacgc tttctgccgc gccgcaggct acttttttcg
      361 ccgtagcgtt gagctgtgcg gagcgatctt tcggcacaat caccgcatga acgcctgcgg
      421 cgtccgcact acgcaggcac gcgccgaggt tgtgcggatc ggttacgccg tcgaggatca
      481 gcaggaacgg ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgac
      541 gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga
      601 gatattggcg gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg
      661 gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg
      721 cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc attaatgtac
      781 tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat ctctgcaata
      841 tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc cggcatgaac
      901 aaagcgcatt ttgtcagcaa tctaaccctc ttcttttaaa gagggtattg atcactctgc
      961 cacttttttc ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc
     1021 cgatggcttt ttcgcttttc tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc
     1081 accgcggaag gcgctgtctg gctcaaagtt taccttttta ccgacctgac aacgcttgcc
     1141 gccttttttg cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg
     1201 cggtgcgcgt tcactggaga tcagactaaa gtcgattttg cgctcgtcca tattaaccgc
     1261 ttcgacgcga acttccacgc gatcgcccag gcgataagtc tggccgctgg attcccccat
     1321 caggcgttgc cctacctggt caaagcgata gtagtcattg tccagcgaag agacatggac
     1381 cagaccatca atgaacaagt cgtccagacg gacgaagaag ccaaagccag tgacgctgga
     1441 aattacgcct ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc
     1501 agcgacatcg cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag
     1561 ttgcaacatc tcttccatcg aataatggta gccgccggtt tcagtggtgt tgccctgatg
     1621 cccctgctct ttcgccagca gatatttaat ggcgcggtgc agcgtcaggt caggataacg
     1681 acgaatcggc gaagtaaagt gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt
     1741 ttctggatcg taaatcgcct gtttcatcga gcgtagcagc atggtttgca gcatttctgc
     1801 atcaggacgg tcggcaaccg actccagcag ttccgcgtag tcacgcggtt ccggcttgtt
     1861 accacccggc agctccagcc ccagctccgc cagcactgaa cggaaagagg taatcgcttc
     1921 ggtgctcggc ttgtcgtgaa tacggaacag tgccggttct ttcgctttct caacgaaacg
     1981 cgccgccgag atattcgcca gaatcatgca ctcttcaatt aatttgtgcg cgtcgttacg
     2041 ctgggtctgt tcgatacgtt caatacggcg ttcagcgttg aaaatgaact tcgcttcttc
     2101 gctctcaaat gagatcccac cgcgttcttc acgggcttta tccagcactt tatagaggtt
     2161 atgcaactct tcgagatgct taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc
     2221 ctgcagaata tgccagactt tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc
     2281 gtagaatttg tagcccgtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca
     2341 caggcggtct acctgcgggt tgagcgaaca caggccgtta gagagcactt ccggcagcat
     2401 cgggataacc tgcgaaggga agtacaccga cgtgccacgg ttacgcgctt ctctgtccag
     2461 cggcgttggc ggacgcacat agtagctgac gtcggcaatc gcgacccata aacgccagcc
     2521 gccgccgcgt tttttctcgc agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc
     2581 aatggtgacc agcggtaaat cgcgcaaatc gacacggccc gcttttgctt cttccggcac
     2641 ttcttctttc agccccgcaa cctgttgctc aacagcctgc ggccagatat acggaatttc
     2701 atgggtacgc agagcgatat caaccgccat gccggtgccc atattgtcgc ccagcacttc
     2761 gacgatttta cccaccgctt tggtgcggcg agtcggacgc tgagtcagtt cgactacgac
     2821 cacaaagccc atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag
     2881 acggctatcg tcaggaacca caaagccgac gcccgcttcg gtaaagtagc gaccaacaat
     2941 ctggctggtt tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc
     3001 agcacccagc ggctgcgcca gcacctgatc gccatgaatg caggttttca tctgctcgct
     3061 ggagagatac aaatcatctt tacgcccttc aacccgcaga aagccgtagc catcacggtg
     3121 accaataacg gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacgacg
     3181 agtgaagacc agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag
     3241 ctgctcttcg ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc
     3301 acgtttggtt aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc
     3361 agcttcgcgt tcctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat
     3421 gaagattttc gtcactccac cagcaataat ttataaagcg gttgattctc ttcaaccaaa
     3481 tcggcaagcg tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc
     3541 tgtttcaacc gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat
     3601 aagggctcca gctcgcgcac cacatcacca atacgtatcg cactcgccgg tttacccagg
     3661 cgaatgccgc cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg
     3721 attattttga ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg
     3781 gtcatccgcc cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg
     3841 aaactcgtta actgcacatc aacctcaaaa gggaaatcgg gaaaaaatac atttatattg
     3901 atgataaacc agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc
     3961 gaggatttaa cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac
     4021 tgttagcccg gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacgggtcg
     4081 cgcagaatca tggtttcagt acgatccgga ccggtagaga tgatatcgat cggcacaccg
     4141 gtcagctctt caatacgctt gatgtagttc agcgccgcct gcggcaggcc gctacgatct
     4201 ttcacgccga aagtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct
     4261 ttccagtcgt cagctgccag cggagtggta gtcacttcgc gaccatccgg catacggtaa
     4321 gccacgcaga gtttaacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag
     4381 ccagacaggg agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta
     4441 cgacgacgac gacccgtagt tgcaccgaat tcgttaccct gcttgcagag gaactcgcca
     4501 gtttcatcaa acagttcggc cgggaaagga cctgcaccta cacgagttga gtaagctttg
     4561 aggataccca gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca
     4621 ccagcagtgg tgttggaaga agttacgtac ggataagtac cgtggtcgat atccagcagc
     4681 gtaccctgcg caccttcgaa catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg
     4741 tcagaaacgt caaccaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga
     4801 actttctggt aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat
     4861 tccatcactt ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc
     4921 agaccgcgac gtgccacttt atcttcataa gcaggcccga taccacgacc ggtggtgccg
     4981 atcgctttcg cgccacgcgc tttctcacgc gcgttatcca gagcaacgtg ataatcaagg
     5041 atcagcggac atgcttcaga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc
     5101 agttctttca tctctttcat cagcgcggcc ggagacagca caacaccgtt accgatgatg
     5161 ctggttacat tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg
     5221 ttgattacga gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc
     5281 cgttcagtca gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt
     5341 acgacgacgt tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat
     5401 cgctttttca gagttacagc actttttgca ctcaaaaatg acctcgatca gtctgttttt
     5461 tgttcagcca atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc
     5521 accgccaaaa cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt
     5581 ccatgccttc gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc
     5641 cagccagatt gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct
     5701 gaggatgcgg tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt
     5761 cggcgtcttc atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt
     5821 gccagagaag ctgttctcat aagcacgcag gctacggatg aatgcgtaga agtccggatc
     5881 tttactgaat gcatcagcaa acagtttggc tgcttcggca tcgccttcac cacgcataat
     5941 gcggccctga cgctcagctt ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg
     6001 cagtttttcc gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc
     6061 ggcgcgcata cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttgat
     6121 acgcacatcg acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac
     6181 tttgcccttc gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatctgctgc
     6241 cggggtagta acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc
     6301 gagggtcaga cgaccacggg aatcggtgac gatatctttc acgtccaggc gaccaatttc
     6361 agaacgcaga cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc
     6421 accacccgtt gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagagtc
     6481 gacgatcagg tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg
     6541 aatacgtgcg tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagaccagg
     6601 ctcataaacc aaaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat
     6661 accgcgctca ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat
     6721 gataatcgcg ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc
     6781 tgcgcgttgg cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt
     6841 gctccactgg ttgtggagga agaggctggc ggcagacgca gcagattgct ggcaccgtta
     6901 tcgctcttcg ccgcaggggc gttaccacct ttcagcatct ggtctaacgg cagaaccatc
     6961 aggttgccac ctttatcgtt aaccagcact ttgcgggtgt tacccaacac tttttccatc
     7021 gtctcgatat acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt
     7081 ttagcaaagc gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc
     7141 gcctcttcga ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggaatac
     7201 gcttctgctt cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac
     7261 gccgctttta cttcttccgg cggacgagca gcctggaagt tgacgtccag cagcgtgata
     7321 cccatgtcat acggacgaat cgtctcttcc agttcgcgct gagtatcgct acgaatcacg
     7381 gtacgacctt ccgtcagaat gcggtccatg gtgtatttac cgataactcc acgcagggcg
     7441 ctgtcggtag cctgacgcag gctgtcatcc gggctggtca cgctatacag atatttttcc
     7501 ggattggtga cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc
     7561 agcatcacac cagaagcggc cagttcacgc acggcttcca cgttcaccgg tttgacttcg
     7621 tcgataaacg tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag
     7681 cgtgttacca cgccgcgttc ggcttcttta atggtataga aaccactggc cgcccagata
     7741 atgacaatcg ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct
     7801 tgcgatgaac tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt
     7861 ttgctcagtt tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg
     7921 tttccatttc cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt
     7981 ccgttattac cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc
     8041 cctgttgtct ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat
     8101 caggtaatcg atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat
     8161 acgaacttgc agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag
     8221 ctgataaaaa cgactcctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc
     8281 cacctcgccg gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt
     8341 ctgtgcggaa agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc
     8401 gaaatcttcc agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc
     8461 gatctcttca agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc
     8521 atcaatgacg tgcagcagta atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc
     8581 cgccaccaga tcgtgcggca ggtggcgaat aaaccctacg gtatctgcaa gtacggtttc
     8641 accgacatct gcaacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc
     8701 tgccgcgtag acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt
     8761 atatcccacc agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc
     8821 acgctgcttt tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa
     8881 acgacggtcg gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg
     8941 tctttcaagg tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag
     9001 ctcaacctgc aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag
     9061 gccggtgcgg tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg
     9121 gctcagggca tggtcaaaaa gaacgaccga agcacccatc gctttgacag cttccgcaat
     9181 ttcaactgct ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt
     9241 aatcacctgc aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc
     9301 ttccatatct ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc
     9361 ataacggtca aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg
     9421 gctccccgtg taaaaaaaca gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc
     9481 ggaagtattc tgcgcgctgc taccatgatg gtagttactg ctggtaccgc caccggcgtt
     9541 gttactgtga tgagaaaccg ggcgagatgg gacaacagta gaaatcgcgt gcttgtaaac
     9601 catctggctg accgtgtttt tcaacaggat cacgaactga tcaaaagact cgatttgccc
     9661 ttgcagctta ataccattca ccaaataaat agaaactgga acacgttccc gacgcagtgc
     9721 gttcaggaac ggatcttgta aagattgccc cttagccatt ctctcttttc cttatatgct
     9781 tatttgtact ttgaaccttt cgattctgaa aaaattgcgc acgatacgtc tcaattgtac
     9841 acattcagcc tgcgatagca ccaacaacct gtaatacttc gtcacgcgcc tgttctggtt
     9901 tttcactgtc aagccagtga accccttccc aaccacgcag ccaggttatc tgccgcttcg
     9961 ccaactgtct cgtggcgcaa acacctcgat aaaccatttc atcgtatgag atttcgcctt
    10021 caaggtaaga ccacatctgg cgataaccca cgcaacgaat ggaaggcaag tccgtatgca
    10081 aatctcctcg ggcaaaaagc gcccggactt ctgcttcaaa acctgaagcc aacatctgat
    10141 gaaaacgctg ctcaatgcgt tgatggagca gttcacggct cgccggggcg atggcgaact
    10201 gatgcacctg atacggtaga gcgtctcctg acgtttgcgt cagttccgtt aaagttttac
    10261 ccgaaatgaa aaaaacttcc agtgcccggg aaagcctttg tggatcattt ggatgaatcc
    10321 ttgctgccgc aaccggatct acctcctgaa gttgacgatg caatgactcc caaccttgct
    10381 ctgccgcctg ttgctcaatt ctggcccgca cttccgggtc tgccgacggt agcggcgaca
    10441 acccttccag caatgccttg aaatacaaca tcgtaccgcc cactaacagt gggatccgcc
    10501 ccgccgcggt gatatcggcc atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt
    10561 aagcctgcga cggatcgcga atatccagca atcggtgcgg cgcggcgagt aactcttcag
    10621 cgttcggctt cgccgtcccg atatccatcc ctttgtaaat aaggacagaa tcaacgctta
    10681 tcaactctac tggtaaaatt ttacgcagct caatggctaa cgccgtttta ccggaggccg
    10741 tcggccccat caaaaaaatc gccttaggca ggctcgcctt actgatatca ctcatctttc
    10801 agggctttta tcgccggatg taaatcaaca gattgtaaca gaccacccgg cggcgttttc
    10861 acaagttgcg gacataaccg ttccacgtcc gccagcaggg ttatggcctg tgccattgac
    10921 cactgcgcat gttcgctcat cagatttcgt gcaatccact gcgcaatatt gccaggttcg
    10981 aatacggact gcttcgccag gtagcctatc agttcaggaa tcaagatttg taaattttgt
    11041 tggcgtaagg gtaaaggcac ggccctgatg gtcacatgct gtgcatctga ctggaaatca
    11101 atacccaatt ccgctagggc agactgcgct ttttctaatg ccgatttttc ttcgccagaa
    11161 acttttagcc gcaacggaat cagcagcggc tgggcgcaaa cgggcgcttc acccggcgtc
    11221 aattgtgcct gacgcagcca acgttctgcc actggcaagg ataaaagtga aatgttgcca
    11281 tcgcgctcca gcaacgcaca gtcggaatgg acgatagtca gtacccgacc aaaactctga
    11341 ctgttcgccg caagtgcagg ttcctgcggt tccggcgctt ttggtttttg catcggcgcg
    11401 ggcgtttgca aaagctggcg atacacttca ccttgctgtt tctggtagcc tggctgcgca
    11461 ttcggccagg gggcagccgg acgactgcct gaggcgggcg caggagagta gcgcggagct
    11521 accggctcac gagctgccgg ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc
    11581 gggatggcac gcggtgcggg ttgtggttca tcgtccagcg gtagcggcgt ttccagttgc
    11641 tgttgtagca cgctcagcac gccctgatag ataaaatcat gcaccagacg cgactgatgg
    11701 aaacgcactt cgtgtttggc ggggtgtacg ttgacgtcca cctgatgcgg gtcgatctcc
    11761 agatacaaca caaatgccgg ttgctgatcg gcccccagtt tgtcttcgca ggcctggcgg
    11821 atcgcatgat tgatcaggcg atcgcgcatc atgcgaccat tcacgtagca atactgaatt
    11881 tccgccagtg cgggcgtagt gtgatttgga tcggccaccc agccgcgcag ggtgagatcg
    11941 ccgtgttgcc attcaatcgc cagcgcttgt tcgagaaaag cggtgccgca aatcgcgcct
    12001 aagcgccgtt ctttttgccc gccttccggc actgcgcggt actgacgcac aattttaccg
    12061 ttatgcgaca ggttgatcgt gacgtcgaaa cgcgccagcg caatgcggcg gatgatttca
    12121 tcaatatggc taaattcggt tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg
    12181 tagaacagat ccagcacctc cagcgtcgtt cccaccggat gcgctgccgg tttaaccgtc
    12241 acgtccatgt cgcgtccttc ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt
    12301 gaagtgagcg tcaggcggga aaccgaactg atactcgcca gcgcctcacc gcgaaagccc
    12361 aggctgataa tggcttcgag atcgtccaga gaggcgattt tactggtggc atgacgagcc
    12421 agcgccagcg ccagctcatc ttttttgata ccgcagccgt tatcacgaat gcggataagt
    12481 ttcgccccac cgcgttcgat atcaatatcg atacgcgtcg cacctgcatc gaggctgttt
    12541 tccactagtt ctttgactac cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg
    12601 ttcgccagtt gtggcggtaa gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg
    12661 cgatctggcg tcgtcaccgt actggcagtt tgtgccgttg caccctgcgg cgcagattgc
    12721 atcggatgcg caaggaagta attgcgcagg cctttgtaaa tggcttctgc cagctgttgt
    12781 tggtaatcgt cgctcgccag caaacgttct tcgctgttgt tgctgataaa accggtttcg
    12841 accagtactg atgggatatc cggcgagcgc agaacgccaa ggctggcgtg ttctggtcga
    12901 cgtttatgta tttcgccaat gcgttgcaac tgactgatca tactggtcgc tacatcatac
    12961 cctacccgct gggaatgacc gaactgtaaa tccagcaccg cctggcttaa ataggggtca
    13021 gactgactgt tcgccagcac atcacccgcc ccacccagca gctccgacag tttctcgtgc
    13081 tgctccagcc agctggccat ttcactgttg gcgcgacggt tagagagcac ccatacggaa
    13141 gcgccagtcg cactacggtt cggtgcggca tcagcgtgaa tcgacacgag gaaattggcg
    13201 ttttgcttat gtgccacatc actgcgcccc atcaccgaga taaagtaatc cccgtcacgg
    13261 gttaaaacgc ctttaaacat cggatcgtca ttgagcaaag tacgcaattt acgcgcgatg
    13321 gcgatggtga catttttctc ccgcgtacca ccggggccga tagcgccagg gtcctgaccg
    13381 ccgtgtccgg catcaatagc gatgataatt ttatcgccag tgttagccgt cgcgcgcgct
    13441 gccggacgcg ttaccgtatt actgctgata acacccgtag tgcggttact ttccgtttta
    13501 aacggattgc gcgccggttc gctgacgcgc ggtgcgacaa ccgcaggcgt ttcaacgcgt
    13561 ttcgcaacca cgggcggcgg aggaggcggt ggcggcacat cggcgttaat cgtaaagacg
    13621 acagtgtaat tgctgccatt ctgccgcttc accgcttcgg ttttaccgtt ttcggtaaga
    13681 tcgaccacca gccgtagcgt ttgtgcatct ttaggcgttc cagagcgaat cgccttcacc
    13741 agattattgc cgctgaacaa caacggcagt ccctgaatca tgcccgtttg tttgatatcg
    13801 agcgccacgg tgcgtttgct ttgatggcta aacgcataat caggatcgcc aataaaactc
    13861 aacgttatcc gcgcctgttg attaccgtta gaaacctgaa tatcagagag cgtcgcggca
    13921 cccaccggcg tgcacagcag cagcagcgtc gctaccaacc aatttctgat gcgatacatc
    13981 atcccgccac ctttcaaagg ttaaccggct aaacgcgcca gcaacaattc acccgcagag
    14041 gaaaccgcac tcacgcgcgc ctcacggcct tgtgcctgat aatcaatgtg tatttcgaca
    14101 tccgggtcag gaagaacacc tgtaccttgt tgtggccact ccaccaggca gatggcatcg
    14161 ttggcaaaat aatcgcggat ccccataaac tccagctcct cgggatcggc aaggcggtac
    14221 aaatcaaagt gatagaccat taagttgtcg agcgtatagg gttcgaccag cgtataagtg
    14281 gggcttttga cattaccctg atgacccaga gcctgtaaaa agccccggct aaaggtggtt
    14341 ttacctgcgc ctaaatcgcc atacagatag attacggttg cgccatcgca ggctttcgct
    14401 acccgctcgc ccaggtctaa tgttgcctgc tcatcaggga gcggaattac tcgattcatc
    14461 atggttttta tcagtcactt ccgggttaac aatacgctgt agcgtggaaa agagatcggt
    14521 tgccagcatc ccgcgcgttc caaaacgcgc cgccagtacg tcagctgccg caccgtgcgc
    14581 gacacagcct gcacaggctg catcatacgg cgacagtttt tgcccaagca atgcgccaat
    14641 aataccagag agcacatcgc ccatgccgcc gctcgccatg cctgcatttc cggcatcaat
    14701 aatgcctaaa gcgtcaggat gggcggcgac cacggttccg gcacctttca gcaccgctac
    14761 gccgccataa cgttgtacca gacgtttggc gcaatgtaag cggtcacttt caatttcagc
    14821 gacggaacag cctaacaacc gtgcggcctc gctaggatgc ggcgtgatca cgcgattgtg
    14881 acgcttatcg ggattgattg ccagcaggtt caatgcatcg gcatcccaca acatcggttt
    14941 gcgaaaattc tcaacttttt gcagggcttt tttcccccac tcttgctggc ccagaccggg
    15001 accaatcacc accacatcgg cccattccag gctttcggta agagagtcca tagtcagttc
    15061 atgcaccatc aattctggtc gtgcagtcag cagcggcgca atgttctcac tgcgggtcag
    15121 tactcggact aaaccagcac cagcacgtag cgccgcttcc cccgtcatac gaatagcccc
    15181 cgccgtgccg tgatcgccac cgataatcac cagccgcccg tgatcgcctt tatgcgaagt
    15241 cgggcgacgc ggtttcagcc agtgagaaag ttgttctgcc gaaaaccgct gaattttcgt
    15301 ctcctgacct gccagccagc tatccagccc cagtgagtca aaatgcagtt gtcctgtaac
    15361 atcccgcgct tttccagtga gcaagcctag tttcagcgca ataaaagtga tggtgtgatc
    15421 ggcgttgatc actgcgcctg gcgtagcgcc ggtttcagcc agcaggccgg aagggatatc
    15481 aaccgccaca atcggcgcag gatgggaatt agcgtggtcg attaactggc taatggattc
    15541 gcggggcgct tgctgcaagc cggtaccgag cagcgcatca acaatcagat ctaccgattc
    15601 gggccagaca atattcgaag catggatctc gccacccgcg tttaaccatg cttcgcgtgc
    15661 cagcgcggcc tcttccggca acggtttgtc gctctcctgg gccaacaacg tgacctcaat
    15721 gccgaccgct ttggccagtc gcgcgaccac gtagccatcg ccgccgttat taccatgacc
    15781 gcacagcacc agc
//
LOCUS       DADLTY010000095        15575 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.095, whole genome shotgun sequence.
ACCESSION   DADLTY010000095 DADLTY010000000
VERSION     DADLTY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.095"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(35..1525)
                     /gene="glpK"
                     /locus_tag="JN334_002555"
     CDS             complement(35..1525)
                     /gene="glpK"
                     /locus_tag="JN334_002555"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAY7438657.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            complement(1565..2410)
                     /gene="glpF"
                     /locus_tag="JN334_002556"
                     /pseudo
     CDS             complement(1565..2410)
                     /gene="glpF"
                     /locus_tag="JN334_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            2836..3081
                     /gene="zapB"
                     /locus_tag="JN334_002557"
     CDS             2836..3081
                     /gene="zapB"
                     /locus_tag="JN334_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HAY7438658.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            complement(3166..3651)
                     /gene="rraA"
                     /locus_tag="JN334_002558"
     CDS             complement(3166..3651)
                     /gene="rraA"
                     /locus_tag="JN334_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HAY7438659.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(3744..4670)
                     /gene="menA"
                     /locus_tag="JN334_002559"
     CDS             complement(3744..4670)
                     /gene="menA"
                     /locus_tag="JN334_002559"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAY7438660.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            complement(4737..6068)
                     /gene="hslU"
                     /locus_tag="JN334_002560"
     CDS             complement(4737..6068)
                     /gene="hslU"
                     /locus_tag="JN334_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HAY7438661.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            complement(6078..6608)
                     /gene="hslV"
                     /locus_tag="JN334_002561"
     CDS             complement(6078..6608)
                     /gene="hslV"
                     /locus_tag="JN334_002561"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAY7438662.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            complement(6701..7660)
                     /gene="ftsN"
                     /locus_tag="JN334_002562"
     CDS             complement(6701..7660)
                     /gene="ftsN"
                     /locus_tag="JN334_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HAY7438663.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            complement(7752..8777)
                     /gene="cytR"
                     /locus_tag="JN334_002563"
     CDS             complement(7752..8777)
                     /gene="cytR"
                     /locus_tag="JN334_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HAY7438664.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            complement(8933..11131)
                     /gene="priA"
                     /locus_tag="JN334_002564"
     CDS             complement(8933..11131)
                     /gene="priA"
                     /locus_tag="JN334_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAY7438665.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            11334..11546
                     /gene="rpmE"
                     /locus_tag="JN334_002565"
     CDS             11334..11546
                     /gene="rpmE"
                     /locus_tag="JN334_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAY7438666.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            complement(11607..12215)
                     /locus_tag="JN334_002566"
     CDS             complement(11607..12215)
                     /locus_tag="JN334_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HAY7438667.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(12399..12716)
                     /gene="metJ"
                     /locus_tag="JN334_002567"
     CDS             complement(12399..12716)
                     /gene="metJ"
                     /locus_tag="JN334_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HAY7438668.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            12993..14153
                     /gene="metB"
                     /locus_tag="JN334_002568"
     CDS             12993..14153
                     /gene="metB"
                     /locus_tag="JN334_002568"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAY7438669.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            14156..>15575
                     /locus_tag="JN334_002569"
     CDS             14156..>15575
                     /locus_tag="JN334_002569"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAY7438670.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRW"
BASE COUNT         3566 a         4172 c         4110 g         3727 t
ORIGIN      
        1 atcagcatca ggcaatcgat tcaggtgcct gacattactc gtcgtgttct tcccacgcca
       61 tcgcgcgttt gaccgctttt ttccagcctg cgtaacggta attacgctca gtggtttcga
      121 tgcctggacg gaactcgcgc tcaatcaccg ctttctcttg cagctcgtcg aggttctgcc
      181 agaagccaac cgccaggcct gcgagatagg ccgcacccaa tgcggtgact tcgcgcactt
      241 ccgggcgctc aacgcgggtg ccgagaatat cggactggaa ctgcatcagg aaattgtttg
      301 ctactgcgcc accatccacg cgcagggcgt gcagacggat accagagtcg gcctgcatcg
      361 cttccagcac gtcacgcgtc tgataagcaa tagactccag cgtcgcgcgt ataatgtggt
      421 tagcgttcac cccacgagtc agaccgaaaa tcgccccgcg cgcatacggg tcccagtacg
      481 gcgcacccag cccggtaaat gccggaacca catacacacc attggtgttt tgcactttgg
      541 tggcgaaata ttcggaatcg taggcgtcgt taatcaactt catctcatcg cgcagccact
      601 gaatggatgc gcctgccata aacaccgcac cttccaacgc atagttcact tcgccagtcg
      661 ggccgcaggc gatggtggtc agcaggccgt tttctgattt caccgctttc tcgccagtgt
      721 tcatcagcat aaagcagcca gtgccatagg tgttcttcgc catcccttct ttcacgcaca
      781 actgaccaaa cagcgcggcc tgctggtcac cggcgatccc ggagattgga atacgcgtgc
      841 cgcctttgcc gccaatgtta gtctgaccgt atacttcgga agaacgacgc acttctggca
      901 gcatctcgcg cggaatatcc agcacttcca gcattttgtc gtcccagtcc agggtatgga
      961 tgttgaacaa catggtacga gaggcgttgg tgtaatcggt cacatggaca cggccctgag
     1021 tcattttcca gataagccac gtatcaaccg taccaaacag caattcacca cgacgtgcac
     1081 gctcgtgaga gccttccaca tggtcgagga tccacttcac tttggtgcca gaaaagtacg
     1141 ggtcaatcac cagaccggta ttgctgcgga tataatcttc taaaccgtca cgttttaaat
     1201 gctcgcagat ttctgcggta cgacggcact gccagacaat ggcgttatag ataggcttgc
     1261 cggtttcttt ttcccagaca atagtggttt cacgctggtt cgtaataccg atagctgcaa
     1321 tttgatcgga actgatatcg gctttcgcca gcacttctac cagcgtggag ctttgggtgg
     1381 cccagatttc cattgggtcg tgttctaccc aacctggttt tgggtagatt tgctcaaatt
     1441 cgcgctgcga cacgctaatg atattggcat cgtgatccat tacgaccgcg cgggagctgg
     1501 tggtgccctg gtcgagcgca acgatatatt tttttcagtc atgtttaatt gtcccgtagt
     1561 catattacag cgaagctttt tgttctgaag gagttgtggt ttccttttct tccacaacac
     1621 agatatcgca aggcaaatgg cgaccaatca gtttgcggta ggcaaatgca cctacaatcg
     1681 cgccaacgat agggctgaaa agcggcacca ggaagtaagg aatgtctctg ccgccggtaa
     1741 aggcgacatt gccccagccc gccagctagg caaagacttt cggaccgaag tcacgcgctg
     1801 ggttcatggc aaaacctgtc aatgggccca tagatgcgcc aatgaccgca atcagtagac
     1861 caatcagcaa gggagccaaa gggccgcgtg gtacaccgtt gccatcgtcc gttaacgcca
     1921 ggatcagccc catcagaata gcggtaatca ccatctcaac tgcgaaagcc tgcacaaaat
     1981 tgatatgagg attagggtaa gtagagaaag tgccagccag atcaacactt tcaacgctgc
     2041 cgcgaacaat gtgatgagtc tgctcgaagt cgaaaaataa attgtagtaa agcccgtaaa
     2101 ctaaagccgc agcgcagaaa gcgccggcaa cttgtgaaac gataaaagga ataactttgc
     2161 gcttgtcgaa acaggcaaac agccacaatg caatggtaac agcgggatta agatgcgcgc
     2221 cggaaacccc tgcggtcagg tcgatggcca ttgccacccc cagtccccaa atgacactga
     2281 tttcccactg accaaaagac gcaccagcga cttttagtgc tgcaacgcaa cccacaccga
     2341 aaaaaatcaa caacccggta ccgaggaatt cagcaatgca ctggcctttc aaggttgatg
     2401 tttgactcat aatcggatcc tgaagagtta atgtttgttg tatgcgtgaa agtcacggac
     2461 ctccacgatg cttgtaggca tgctgttaac ttatcgttaa cgagcaaaaa cgagaaatat
     2521 cgaacttaaa atgtgtgtgc ttcgtcataa aatgagcgtt atcgcgccat ttattccatc
     2581 tgattgtaca tgatgcatgt ttgttagatc attcgcgcaa caaatttatt aacattttcg
     2641 attgatgtgg attatgtcac taggagaagg atgaaaagtg tggcaaaccg taatctgcaa
     2701 ggcgtgccgc tggacagcga tggcgcggct tcatacaatc ggagctaact aaagtgcgct
     2761 cgtatttatt aaggcgtcac cggtaatcgg gacgaggatt ttttatccat caacgccttg
     2821 caattcagga gaggtatgac aatgtcatta gaagtgtttg agaaactgga agcaaaagta
     2881 cagcaggcga ttgataccat cactctgttg cagatggaaa tcgaagagct gaaagaaaaa
     2941 aacaactcac tgtcgcagga agttcaaaat gcccagcatc agcgcgaaga gctggagcgt
     3001 gagaacaacc atctgaaaga acagcagaac ggctggcagg aacgtctgca ggccctgctg
     3061 ggtcgcatgg aagaggtctg attacgcttc atctttcgcg taatttcttt actgtgtagg
     3121 ccaacaggta acgcagaaaa aaggcacctt gcggtgcctt tcttatcatt caatatccag
     3181 cggatcttct gaaagaataa tcccggtatt gtcggcataa agatggtcgc cggagaagaa
     3241 ggtgacaccg ccaaaattga cgcggacatc gctttcgcca atgccttcgc cagcggcacc
     3301 aaccggaatt gccgccatcg cctggatgcc gatatccaac tcttccaggt catctacctg
     3361 acgcaccgcg ccgtaaatga ccagaccttc ccattcattt tgtactgcca gacgcgccag
     3421 ttcagcatcg accagtgcgc gacgaacaga accaccgcca tcgacgacaa gaacacgacc
     3481 acggccattc tgttcgagca gatcgtacag caacccgttg tcctcgaaac attttaccgt
     3541 gattatttgt ccgccaaacg acgcccgtcc gccaaagttg gagaacagcg gttccacgac
     3601 gttaacatct tcttgataga tgtcacaaag ctcggaagta tcgtatttca taggcttaac
     3661 attcagttgc tgcgagaatt ttcagtatat cgcgctatgt gggctgttgg caaaatcatc
     3721 aattgttaat tgatatttgt cagttatgct gcccactggc ttaggaatat ccctaaaaca
     3781 aacagcaggt tagtcagtaa cgctcccttg acagtacgtt ccagcattgg tcgcatcgcc
     3841 accgggtcca tttcccgcat cacataacgg gcttgcttca ccagtaatgg tgccgccagc
     3901 aggaacagcc agccccacag gctatgcagc gaaaagagat taaacagcgc cagacacacc
     3961 agcgagccca tcagcaggca ggcatgataa cgacgcgcgt tcacttcacc taagcgcacc
     4021 accagcgtgt ttttgccatt ttcgcggtcg ctattgatat cacgcaggtt attaatattc
     4081 agtactgccg ttgccagcag gccgcatgcg gtcgccggaa ggatcagtgc cggaatcaat
     4141 gtatgagcct gtaaatacca gctccccatg acactcaacc agccaaagaa aaccagtacg
     4201 gaaatatcac ccagaccgat ataaccataa ggacgattgc ccacggtgta ggtgatagcg
     4261 gcaatgatcg acaacccgcc aagaatcagg aaaccgacaa aatcggccag cgtatggcat
     4321 gccactgcaa ccagtgccag cccggagaga cagatgagca cgacggtaat aatgagcgcc
     4381 cgtttcatct tttgctgggt aatgacccct ttttgcatgc cgcgtagcgg cccaatgcgg
     4441 tcaggtttat cgctgccttt taccgcatcg ccgtaatcat tggcgaggtt agaaaggatc
     4501 tgtaatagcc cggcggtaat tagtgccagc agggcgacca gcggatcgaa gtgaccttgc
     4561 caccatgcca gcgccgtccc gacgataatt gcagcaaagg cgagggggag ggttttaggt
     4621 cgtaaacttt ccagccacgc ctgagttcgg ctaatttgtt gttcagtcat aatacgcgcc
     4681 aataaaaatg gggcctttca gccccatcaa acaatgatga aaatgattga acgcgattat
     4741 aggataaaac ggctcagatc ttcatctgcc accaacgcat ccagatgttt gctcacataa
     4801 tctgcgtcaa tagtgatatt ttgaccgctt aaatcgctgg cgtcgtagga aatctcttcc
     4861 attaaacgct ccagaacagt gtgtaaacga cgagcaccga tgttttcggt agattcgttc
     4921 acctgccatg ccgcttccgc gatgcgttta ataccggagt cggtaaactc gatatttacg
     4981 ccttcagtcg ccatcagtgc tttgtactgc acggtgatag aggcattcgg ctcggtcaga
     5041 atacgctcga agtcgctggt ggtcagtgcc tgcagttcaa cgcggattgg cagccgacct
     5101 tgcagttccg ggatcaggtc agacggtttc gcaatctgga acgcgccaga agcgataaac
     5161 agaatgtggt cagttttgac catcccgtgt ttggtggaaa cggtgcaacc ttctaccagc
     5221 ggcagcaggt cacgctgaac gccttcacga gaaacatccg gaccggaaga ctcgccgcgc
     5281 ttacagattt tgtcgatttc gtcgataaac acgatcccgt gctgctcaac agcgtcgata
     5341 gcgtcttgct tcagctcttc cgggttcacc agtttcgccg cttcttcttc aatcagcagc
     5401 ttcatggcgt ctttgatttt cagcttacgc gctttttgct tctggccgcc caggttctgg
     5461 aacatggact gcagctggct ggtcatctct tccatgcccg gaggagccat aatttcaacg
     5521 cccatcggtg ctgcggcaag atcgatctcg atttctttgt catcaagctg gccttcacgc
     5581 agttttttgc ggaatgcctg acgagcagcg gaaggttcct gctgctgttc agtctgtccc
     5641 cagttgtttt tagcaggtgg gatcagcacg tcgagaatac gttcttctgc cagttcttca
     5701 gcgcgataac ggtttttctc gatagcctgg acgcgtacca ttttcacggc ggcatcggtc
     5761 agatcgcgaa taatagaatc aacttcctta ccgacgtagc ccacttcggt aaatttggtc
     5821 gcttcaactt tgatgaacgg cgcattcgcc agcttagcca gacgacgggc gatttcagtt
     5881 ttaccgacac cggtcgggcc gatcatcagg atatttttcg gggtcacttc atggcgcagc
     5941 tcttcgttga gctgcatgcg acgccagcgg ttacgcagag caatcgccac agaacgcttg
     6001 gcgttgtcct ggccgatgat gtgcttatcc agttcgctga cgatttcgcg tggggtcatt
     6061 tcagacatgg gagatcctta cgctttgtag cttaattctt cgatggtgtg gaaatggttg
     6121 gtatagatgc aaatgtcgcc tgcaatatcc aacgcctttt cagcaatttc acgggcgcta
     6181 agttcagtgt tttctaacag cgcgcgcgcc gcagcctggg cgtaagggcc gccggagccg
     6241 atagcaataa gatcgttttc tggctgcacc acgtcaccgt taccggtgat gataagcgat
     6301 gcagtttcat ccgcgactgc cagcagtgct tcaagtttgc gcagcatgcg atcggtacgc
     6361 cagtcttttg ccagctcaac ggcggcttta accagatggc cctgatgcat ttccagttta
     6421 cgttcaaaca gttcgaacag cgtaaaagca tccgcagtac cgcccgcaaa gcccgcgatg
     6481 actttgtcgt tgtacagacg gcggaccttt ttcacgttgc ctttcattac ggtattgccc
     6541 aacgtggcct gaccatcacc agcgatgacc acatggccgt tacggcgtac gcttactata
     6601 gttgtcacga gctgacccct tggttacgaa tacagagtac aaaccccgta caaaagtacg
     6661 gggcataatg caattataga tgggggggat tttgagggtt tcaacccccg gcggcgagcc
     6721 gaatgcagtt tgtatgaccc gccatcttca accgattgag ggtgctgtct gcgttctctt
     6781 tgcctttcac cgggccaatg accacacgat tccagccatt gttggtggtg attttcgagt
     6841 caaatccttc gaacgccagc tgagcacgta ccgtctctgc ctgttccgcg cctctgaacg
     6901 aaccgcactg caccatccag cggcgttcgt cttttttctc cgccgtcggt tttggcgcgt
     6961 cagcggcacg agcaaccggc gcagcttgct gcggcttcga ctgcgcggtc gtgtgcgcag
     7021 gggtttgcag cagatcctgg tacggctgct gagtagaagc tggtttagac tggcgcggtt
     7081 gcgcctgtac cggcgcggct tgcgacgtac gcgtctgctg ctgccagctt tgttcagtcg
     7141 tacgcgactg ctgagccagg cgttgctgct ccgctaattg ctgcgcctga cgctggcgtt
     7201 gtagcgtttg ctgacgctgt tccggcgtct gttcattcca cggaacctca accagctgcg
     7261 ttggctgctg gcgcatatca gcctgcattt gttcaagaag ctgacgttgt tctggtgtca
     7321 gttgctccgg cgttttcact tcaccaccgg cagaaggttc tgtgggcgca cgcacacccg
     7381 gctggcgact ttccagctct ttaatgtagc gccagcgttc ttctggtttt ggtggtagtc
     7441 cgtttccggt cactttctgg ctttgcagcg tctcggactc ttctttcttg tgatgcgtaa
     7501 tgaagtacag accaccgata aaggtcacaa gaacggcggc agcaatagcg accatagcgg
     7561 gagaaaccgc aggcagattt cgttgctttt tccgtgaggt gctctttttt cgccgcgaag
     7621 gtgccggttg gctgcggcgt acataatctc gttgtgccac tatcgtttcg ctgtatttat
     7681 tcgttcgtca gcccgccatg ttacttaagc ggcgggcctt tgaccagtca cggcagtctt
     7741 aaaggtttac tttaaggtaa cgcgcgtgtt gatccccgga tgataagttc gcagtccatt
     7801 aaacgagagc cactgccaac gtgttgcccc tgcatttgat caagcaataa cagcatagct
     7861 tcccgaccga tttcgtaacg cggctgcgcg atggttgtca gcggcggatc acaaaattgc
     7921 gtcaggtcga tgttatcaaa accgattatg gaaaggtctt ccgggacttt cagcccctgg
     7981 cgttttgcct gagaaagtgc gccgagcgcc atcacatcgc tatggcagaa gactgcagta
     8041 ggcggttgtg gaagatcaag cagctgttgc attgctttgc ttccggcttc gaaggtgaag
     8101 tcgccacggg cgatgtattg tggatcaacc ataattccgc agcgacgcag cgcctgaaca
     8161 tagccttgca ggcggtagtg acacagtggc atctcttcgg gaccggctat acagccaatc
     8221 cgtttatgcc cttgctcata taaataattt actgcatcaa atgcggcggt cagattgtcg
     8281 atatgaacgg taggcagctc cagttccggt gcaaattcgt tcgccatcac catcggcggc
     8341 agattacgct gttcctcaat gctggcatca aacggcagcc ttgaacccag cagcaacatg
     8401 ccatcaattt gcttggtgat gatcaaatcg ataaaggttt tttcctgctg attttgatgc
     8461 gcacagtcgc caatcagcac cagatatccg tgatttgccg ccgtaacttc gataccgcga
     8521 ataatttcgc taaagaaggg atcgcagata tccgggacaa tcaccagaat ggtgcgggat
     8581 tcattacgct tgacgttgcg ccccataggc tgcggtaaat aacccacttc ccgggccgct
     8641 ttttcaaccc gattacgggt ggcctgggag actttatcgg gattcattaa tgctcgggag
     8701 acagtcgctg tagagacttt tgccttgagg gcaacgtctt tcatggtcgc ggcagtttcc
     8761 tgcttcttcg ctttcacact cactcctcgc ctggcacgtc aggcgtacta catccatgtt
     8821 tacttcacat cggcaacatt tttagcagat agcgcgtgaa aacggttaca gaattttcat
     8881 gaaaagtgtg atgaatattg aatttttcga tccgcctcgc atcgtaagcg gtttaaccct
     8941 caatcggatc aacatccagt acccatttca ccttgcggga atccggtatt gtattgatga
     9001 gcgccagcgt accactaatg atgtgttgca agcgcacgcg ggaagggtgc tgcaacaata
     9061 tctgccagcg ccagcgaccg ccacgtttag gtgccagtgc cggaaccgga ccgagaaccc
     9121 acagtttgtc gtctgccagc gggctggaga ggatcagatt acgcagttgt tgcaggaaca
     9181 gcggcgcatg ctgattgtta tgatcttccg cacgcacaat cacatggctg gtccatggcg
     9241 gcagttgcat catccgtcgc tcagccagcg cctgttcggc aaaggcatcg tagcctttat
     9301 agagcaacgt ttgcaacaga ggatgttccg gatggtgcgt ttgcagcacc acttcgcctt
     9361 gtttacccgc acgcccggca cgaccagcga cctgggtgta aagctgagcg aaacgctctg
     9421 ccgagcgaaa atcggcagaa aacagcgcgc cgtccacatc cagtaacgcc accagcgtca
     9481 catccggaaa gtggtgacct ttcgccagca tttgtgtgcc gatcaaaatc ctcgcaccgc
     9541 cgcgatgtac ttccgccagt tgctgttcca gcgccccttt gcggctggtg gtatcgcggt
     9601 cgatacgaga aatgggcaca tcagggaaca gcggcgcaag cgtctgttca agctgttcgg
     9661 tgcccagccc cacgggtacc aggtgcgtgg aaccgcagga agggcactgg cgcggcaccg
     9721 gacgctgact gtcacagtgg tgacagcgca aatgctgttg cgcctgatgc agcgtgtagt
     9781 agtgatcgca acgtgggcat tcggcaatcc agccgcagtc gtggcacagt agcgcaggcg
     9841 caaagccacg gcggttaaga aagaggataa cctgattgtt ggcctgtaaa tgctggcgca
     9901 tacgggtgat taacgccgga gccagacctg cctgcacctt ctgacctttt aaatccagca
     9961 cgtgttgaat tgccggacgc gcattccccg cccggcgcgt caggcgcagc aggcggtatt
    10021 ttttctgctg gacgttgcac aacgtttcca gcgcgggcgt tgcggagcca agaataatcg
    10081 ggatttgctc gctatgcgca cgatacaccg ccaggtcgcg ggcatgatag cgccagcctt
    10141 cctgctgctt gtaggaactg tcgtgctctt cgtcaatgac aatcacgccg agatttttaa
    10201 acggcgtaaa cagcgcggag cgggtgccga tcacaatcgc cgcctcgcca tttttcgctt
    10261 tcagccacgc cgaaagacgc tcgctgtcgt tcagaccgga atgcagaact tccacgggcg
    10321 cattaaaccg ttcacgaaaa cgggcgatag tttgcggtgt caggccgatt tccggcacca
    10381 tcaccagcgc ctgtttgccc tgagcgagca cgttttccag tacactgaga taaacctccg
    10441 ttttaccgga accggtaacg cccgccagca gccaggcaga aaaagtatct gccgcgctat
    10501 gaattgcgcc aacggcggtg gcctgttcgg tattcaatcg caaccgctca ccagaaacgg
    10561 catagttcgt tcgccagtcg ctaaactctg gtgtttcact tgctaaatca cacagacctt
    10621 ttttgcgcag cgcctgcaac gcggcatcat taaattcgag cgtggcaacc tggtcgcgcc
    10681 agattttgcc ttgccgtaac gctgccagcg cctgttgttg cttgggtgag cgtttcaggc
    10741 tgttcagatc caccgcctga ccttgttcag tggcaaacca gtaccacatc ggcgcgttcg
    10801 ccgcaggccg cccctggcgt agtaaaatcg gcaaggcatg aaacagcaca tcgccaatcg
    10861 gatgatgata gtaatctgcc gcccatagca gcaatcgcca gacggagtga gtaaacaccg
    10921 gctcaccatc cagcacttcg actaccgctt ttagctcagt gagcggcagt tcgctggcat
    10981 cgctaactga taccacaatc ccgatgcgct cctgctgttt gccaaacggc acgcgcacgc
    11041 gacacccagc tttaaccgtc atgccttctg gcagcagata gtcaaaggta cgaggaagcg
    11101 gaacgggcaa ggcaacgtgg gcaacgggca tagcatcatc ctgacttgaa attcggtggg
    11161 ttagtataca cattgccgta gaagagtgcg gatcagtttg catacgctgg ttaatttctg
    11221 tatgatttcg cgccttcgta cgaaatgatc gtattgaagc tatactttta acatcgcgtg
    11281 gtgtctggcg ttagggctgg aagagcgacg cggccttaaa ccgaggtttt cccatgaaaa
    11341 aagatattca cccgaaatac gaagaaatta ctgctagctg ctcttgcggt aacgtaataa
    11401 aaatccgctc caccgttggt catgacctga acctcgacgt gtgcagcaag tgccacccgt
    11461 tcttcactgg caaacagcgt gatgttgcta ccggtggccg tgttgaccgc ttcaacaagc
    11521 gtttcaacat cccgggcagc aaataagacc ggatttccga aaaaagcgcc gcacggcgct
    11581 ttttttgtgc ctgaattttg gtcgtatcac cacgaaaaca gcggccactc tttggcaacc
    11641 gtggtgagtt gtggcgcgtc gaaaacggct tgtggtgaaa ctaccgtttc ctcaagcgga
    11701 atatttttac cgtagcgttc tttgagcttc gcctggacca gcgggttgct gagatcaaac
    11761 tctttcaact tctgctgttc gcccacgcgc attcccagcg cgttctggta aaccttccac
    11821 accacttccg aacagtactg gcgatcgtcg ctccatgaga agctgaaatc atacggttta
    11881 ccgagataac gttttgccgt ttgcgtcagt ttttgctgtt gttcaacact cagtccgcct
    11941 tcaacgcggc gaacaacgta gttgcccttt tcaccatggg caatccactg cttgagcggg
    12001 gtgtatttca ccgggccgac cgcttcaaaa acgtagggct ttttgttacg catcaccagc
    12061 ataccggtgt ggctgtaatc ggaatgggtt gccagttgga tcgctttact ttgcgatgag
    12121 cgagagattt gaaagatgat gtcgccagtt tgcggctgcc aggcaaaggc aggtacagaa
    12181 accagcaggc tgaggatcag cagcctgttt ttcatagtta aacgtccatg tataaaaagc
    12241 ggtgggtcgc agacaacgtg ctcgttgttt atgccggatg cggcgtgaac gccttatccg
    12301 gcctacaagt tcgtgcaaat tcaataaatt gcaatatgac gtaggcctga taagcgtagt
    12361 gcatcaggcg attccactcc gcgccgctct tttccgcttt agtattccca cgtctccggg
    12421 ttaatcccca tctcacgcat gatctctttt gccgcttccg ggatttcgtc gctgcgctct
    12481 ttacgcagat cggcatcatc cggcaaaggt tgcccggtaa aggcatgcag aaacgcttcg
    12541 cacagcagct cgctgttggt agcgtgacgc aggttgttca cctgacgacg cgtgcgttca
    12601 tcggtgagga tttttaacac cttaagagga atggaaaccg taatcttttt gacttgttca
    12661 ctcttcttgc cgtgctcagc gtatgggctg atatattcgc cgctccattc agccatgaga
    12721 tacttaatcc tcttcgtcaa taaattgaga ccagacccca caatttatgt gggtactgac
    12781 cgtaaacccg catagtttac cgtacaggcg ttaccgtgac atcgtgtaat gcacctgtcg
    12841 gcgtgataat gcatataatt ttaacggcta tttgggattt gctcaatcta tacgcaaaga
    12901 agtttagatg tccagatgta ttgacgtcca ttaacacaat gtttactctg gtgcctgaca
    12961 tttcaccgac aaagcccagg aaacttcatc acatgacgcg taaacaggcc accatcgcag
    13021 tgcgtagcgg gttaaatgac gacgaacagt atggttgcgt tgtccctccc atccatcttt
    13081 ccagcaccta taactttacc ggatttaatg aaccgcgcgc gcatgattac tcgcgtcgcg
    13141 gcaacccaac gcgcgatgtg gttcagcgtg cgctggcaga actggaaggc ggtgctggcg
    13201 cagtacttac taataccggc atgtccgcga ttcacctggt aacgaccgtc tttttgaaac
    13261 ctggcgatct gctggttgcg ccgcacgact gctacggcgg tagctatcgc ctgttcgaca
    13321 gtctggcgaa acgcggttgc tatcgcgtgt tgtttgttga tcaaggcgat gaacaggcat
    13381 tacgggcagc gctggcagaa aaacccaaac tggtactggt agaaagccca agtaatccat
    13441 tgttacgcgt cgtggatatt gcgaaaatct gccatctggc aagggaagtc ggggcggtga
    13501 gcgtggtgga taacaccttc ttaagcccgg cattacaaaa tccgctggca ttgggtgccg
    13561 atctggtgtt gcattcatgt acgaaatatc tgaacggtca ctctgacgtg gtggctggcg
    13621 tggtgattgc taaagacccg gacgttgtca ctgaacttgc ctggtgggca aacaatattg
    13681 gcgtgacggg cggcgcgttt gacagctatc tgctgctacg cgggttgcga acgctggtgc
    13741 cgcgtatgga gctggcgcag cgcaacgcgc aggcgattgt gaaatatctg caaacccagc
    13801 cgttggtgaa aaaactgtat cacccgtcgc tgccggaaaa tcaggggcat gaaattgccg
    13861 cgcgtcagca aaaaggcttt ggcgcaatgt tgagttttga actggatggc gatgagcaga
    13921 cgctgtgtcg tttcctgggc gggctgtcgt tgtttacgct ggcggaatca ttagggggag
    13981 tggaaagttt aatctctcac gccgcaacca tgacacatgc aggcatggca ccagaagcgc
    14041 gtgctgccgc cgggatctcc gagacgctgc tgcgtatctc caccggtatt gaagatggcg
    14101 aagatttaat tgccgacctg gaaaatggct tccgggctgc aaacaagggg taaaaatgag
    14161 tgtgattgcg caggcagggg cgaaaggtcg tcagctgcat aaatttggtg gcagtagtct
    14221 ggctgatgtg aagtgttatt tgcgtgtcgc gggcattatg gcggagttct ctcagcctga
    14281 cgatatgatg gtggtttccg ccgccggtag caccactaac cagttgatta actggttgaa
    14341 actaagccag accgatcgtc tctctgcgca tcaggttcaa caaacgctgc gtcgctatca
    14401 gtgcgatctg attagcggtc tgctacccgc tgaagaagcc gatagcctca ttagcgcttt
    14461 tgtcagcgat cttgagcgcc tggcggcgtt gctcgacagt ggtattaacg atgcggtgta
    14521 tgcggaagtg gtgggccacg gtgaagtgtg gtcggcgcgc ctgatgtctg cggtacttaa
    14581 tcaacaaggg ctgccagcgg cctggcttga tgcccgtgag tttttacgcg ctgaacgcgc
    14641 tgcgcaaccg caggttgatg aagggctttc ttacccgttg ctgcaacagc tactggtaca
    14701 acatccgagc aaacgtctgg tggtgaccgg gtttatcagc cgtaacaacg ccggtgaaac
    14761 ggtgttgctg gggcgtaacg gttccgacta ttccgcgaca caaatcggtg cgctggcggg
    14821 tgtttctcgt gtaaccatct ggagcgacgt cgccggggta tacagtgccg acccgcgtaa
    14881 agtgaaagat gcctgcctgc tgccgttgct gcgtctggat gaggccagcg aactggcgcg
    14941 tctggcggct cccgttcttc acgcccgtac tttacagcca gtttctggca gcgaaatcga
    15001 cctgcagctg cgctgtagct acacgccgga tcaaggttcc acgcgcattg aacgcgtgct
    15061 ggcctccggt actggtgcgc gtattgtcac cagccacgat gatgtctgtt tgattgagtt
    15121 tcaggtgccc gccagtcagg atttcaaact ggcgcataaa gagatcgacc agatcctgaa
    15181 acgtgcccag gtgcgtccac tggcggttgg cgtgcataac gatcgccagt tgttgcaatt
    15241 ttgctacacc tcagaagtgg ccgacagtgc gctgaaaatc ctcgacgaag cgggattacc
    15301 tggagaactg cgcctgcgtc aggggctggc gctggtggcg atggtcgggg cgggtgtcac
    15361 ccgtaacccg ctgcattgcc accgcttctg gcagcaactg aaaggccagc cggtcgagtt
    15421 tacgtggcag tccgatgacg gcatcagtct ggtggcagta ctgcgcaccg gcccgaccga
    15481 aagcctgatt caggggctgc atcagtccgt cttccgcgca gaaaaacgca tcggcctggt
    15541 attgttcggc aaaggcaata tcggctctcg ctggc
//
LOCUS       DADLTY010000096        15534 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.096, whole genome shotgun sequence.
ACCESSION   DADLTY010000096 DADLTY010000000
VERSION     DADLTY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.096"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            209..403
                     /gene="bfd"
                     /locus_tag="JN334_002570"
     CDS             209..403
                     /gene="bfd"
                     /locus_tag="JN334_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HAY7438671.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            476..952
                     /gene="bfr"
                     /locus_tag="JN334_002571"
     CDS             476..952
                     /gene="bfr"
                     /locus_tag="JN334_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAY7438672.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(949..1416)
                     /locus_tag="JN334_002572"
                     /pseudo
     CDS             complement(949..1416)
                     /locus_tag="JN334_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1795..2106
                     /gene="rpsJ"
                     /locus_tag="JN334_002573"
     CDS             1795..2106
                     /gene="rpsJ"
                     /locus_tag="JN334_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAY7438673.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2139..2768
                     /gene="rplC"
                     /locus_tag="JN334_002574"
     CDS             2139..2768
                     /gene="rplC"
                     /locus_tag="JN334_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAY7438674.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2779..3384
                     /gene="rplD"
                     /locus_tag="JN334_002575"
     CDS             2779..3384
                     /gene="rplD"
                     /locus_tag="JN334_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAY7438675.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3381..3683
                     /gene="rplW"
                     /locus_tag="JN334_002576"
     CDS             3381..3683
                     /gene="rplW"
                     /locus_tag="JN334_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAY7438676.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3701..4522
                     /gene="rplB"
                     /locus_tag="JN334_002577"
     CDS             3701..4522
                     /gene="rplB"
                     /locus_tag="JN334_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAY7438677.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4539..4817
                     /gene="rpsS"
                     /locus_tag="JN334_002578"
     CDS             4539..4817
                     /gene="rpsS"
                     /locus_tag="JN334_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAY7438678.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4832..5164
                     /gene="rplV"
                     /locus_tag="JN334_002579"
     CDS             4832..5164
                     /gene="rplV"
                     /locus_tag="JN334_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAY7438679.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5182..5883
                     /gene="rpsC"
                     /locus_tag="JN334_002580"
     CDS             5182..5883
                     /gene="rpsC"
                     /locus_tag="JN334_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAY7438680.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5896..6306
                     /gene="rplP"
                     /locus_tag="JN334_002581"
     CDS             5896..6306
                     /gene="rplP"
                     /locus_tag="JN334_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAY7438681.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6306..6497
                     /gene="rpmC"
                     /locus_tag="JN334_002582"
     CDS             6306..6497
                     /gene="rpmC"
                     /locus_tag="JN334_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAY7438682.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6497..6751
                     /gene="rpsQ"
                     /locus_tag="JN334_002583"
     CDS             6497..6751
                     /gene="rpsQ"
                     /locus_tag="JN334_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAY7438683.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6916..7287
                     /gene="rplN"
                     /locus_tag="JN334_002584"
     CDS             6916..7287
                     /gene="rplN"
                     /locus_tag="JN334_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAY7438684.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7298..7612
                     /gene="rplX"
                     /locus_tag="JN334_002585"
     CDS             7298..7612
                     /gene="rplX"
                     /locus_tag="JN334_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAY7438685.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7627..8166
                     /gene="rplE"
                     /locus_tag="JN334_002586"
     CDS             7627..8166
                     /gene="rplE"
                     /locus_tag="JN334_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAY7438686.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8181..8486
                     /gene="rpsN"
                     /locus_tag="JN334_002587"
     CDS             8181..8486
                     /gene="rpsN"
                     /locus_tag="JN334_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAY7438687.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8520..8912
                     /gene="rpsH"
                     /locus_tag="JN334_002588"
     CDS             8520..8912
                     /gene="rpsH"
                     /locus_tag="JN334_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAY7438688.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8925..9458
                     /gene="rplF"
                     /locus_tag="JN334_002589"
     CDS             8925..9458
                     /gene="rplF"
                     /locus_tag="JN334_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAY7438689.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9468..9821
                     /gene="rplR"
                     /locus_tag="JN334_002590"
     CDS             9468..9821
                     /gene="rplR"
                     /locus_tag="JN334_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAY7438690.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9836..10339
                     /gene="rpsE"
                     /locus_tag="JN334_002591"
     CDS             9836..10339
                     /gene="rpsE"
                     /locus_tag="JN334_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAY7438691.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10343..10522
                     /gene="rpmD"
                     /locus_tag="JN334_002592"
     CDS             10343..10522
                     /gene="rpmD"
                     /locus_tag="JN334_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAY7438692.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10526..10960
                     /gene="rplO"
                     /locus_tag="JN334_002593"
     CDS             10526..10960
                     /gene="rplO"
                     /locus_tag="JN334_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAY7438693.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10968..12299
                     /gene="secY"
                     /locus_tag="JN334_002594"
     CDS             10968..12299
                     /gene="secY"
                     /locus_tag="JN334_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAY7438694.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12331..12447
                     /gene="rpmJ"
                     /locus_tag="JN334_002595"
     CDS             12331..12447
                     /gene="rpmJ"
                     /locus_tag="JN334_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAY7438695.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12594..12950
                     /gene="rpsM"
                     /locus_tag="JN334_002596"
     CDS             12594..12950
                     /gene="rpsM"
                     /locus_tag="JN334_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAY7438696.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12967..13356
                     /gene="rpsK"
                     /locus_tag="JN334_002597"
     CDS             12967..13356
                     /gene="rpsK"
                     /locus_tag="JN334_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAY7438697.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13390..14010
                     /gene="rpsD"
                     /locus_tag="JN334_002598"
     CDS             13390..14010
                     /gene="rpsD"
                     /locus_tag="JN334_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAY7438698.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14036..15025
                     /gene="rpoA"
                     /locus_tag="JN334_002599"
     CDS             14036..15025
                     /gene="rpoA"
                     /locus_tag="JN334_002599"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAY7438699.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15066..15449
                     /gene="rplQ"
                     /locus_tag="JN334_002600"
     CDS             15066..15449
                     /gene="rplQ"
                     /locus_tag="JN334_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAY7438700.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4007 a         3644 c         4133 g         3750 t
ORIGIN      
        1 gcgttgttgc taaagttctg ggctaattac acgttaatta gttttgaatt gaaaagggcg
       61 cttcggcgcc ctttttgctg cccgtaattt gagctcgaaa tatttagtcg taattttgct
      121 cgctgaacta tggcgcaaat aaaactgtgc tattgtaatc ataaccattc tcatttacac
      181 tttgtgcaga atttgaacgg gagcaatcat gtacgtttgt ttgtgtaatg gtgtaagtga
      241 caaaaaaatt cgccaggcag tccgtcagtt tcatccgcaa tctttccaac aattgcgtaa
      301 gtttattcct gtgggaaatc aatgcggtaa gtgcattcgc gccgcgcgcg aagtgatgca
      361 ggacgagttg atgcagatgc cagaatttaa agagattgcc tgaagcccac gcttattttt
      421 tgacatcctg ctagccccat ctacgctcta atagtggaag cggagggact ataaaatgaa
      481 aggtgatgtt aaaatcataa attatctcaa taaactattg ggaaatgagc ttgtcgcaat
      541 caatcagtat tttctccatg cccggatgtt taaaaactgg ggcctgatgc gtctcaatga
      601 cgtcgaatac catgaatcca tcgatgagat gaaacacgct gataaatata tcgagcgtat
      661 tttgttttta gaaggaatcc ccaacttaca ggatttaggc aaactgggga ttggtgaaga
      721 tgtcgaggag atgctgcaat ctgacctgcg attagaactg gaaggcgcga aggatctgcg
      781 tgaggctatc gcatatgccg acagcgttca tgactatgtt agccgcgata tgatgatcga
      841 gatccttgct gatgaagaag gtcatatcga ctggctcgaa actgaactgg atctcatcgg
      901 taaaatcggc ctacaaaatt acctgcaatc acaaattaaa gttaaagatt aacggcctat
      961 cagcaaacta tcccagccta taacgaaacc cgcagccgcc agaaatggcc caaaaggcag
     1021 cgggtttttt aatgattctt ttcctctcat gagcagacca ataactacgg ctccgcaagc
     1081 gaatgaggca gcaaggaaaa ccagtcgtgg taagaaggcc catgaatgct aggcgccaag
     1141 ggcggcgaga aacttcacat caccatagcc taatccttct ttatgtcgca gtatgcgata
     1201 cccccagtaa atgacagcga atgtgccgta accaacaatt gccccccata acgcatcggc
     1261 taaaccatca ggatgacaaa cttgatagaa caaaagacct gaccagagta acgggcacgt
     1321 aaatctgtca ggtaacaagc catgctttgc atcccagaaa aaaagtaggg ctgataaaca
     1381 agcgtacaaa attagaaagg gaagggtagt gtccataaaa catcctctgt aattggagta
     1441 tcaccatcga ataactccgg atgctcagca acagagagag cgctgatttg ttaacctgat
     1501 tccggtgaaa aacgctatct tcacaacctt acaaatttcg tggaatgcag ctcgcaatct
     1561 gcgcgttgcg attaatttct acccgatgct tgcgtcaggc tcagatttgc gtataatgcg
     1621 cgggcttgtc gtagttgaca gcaggttcaa tctgaacctc agttagccga atttggctac
     1681 ctaacaatgc tcccaatcgg ggagctacgt aagaacggtt acactctccc atcaatcgta
     1741 atgggtctga ggagtaatca ttttcgttta taaaataatt ggagctctgg tctcatgcag
     1801 aaccaaagaa tccgtatccg cctgaaagcg tttgatcatc gtctgatcga tcaagcaacc
     1861 gcggaaatcg tcgagactgc caagcgcact ggtgcgcagg tccgtggtcc gatcccgctg
     1921 ccgacacgca aagagcgctt cactgttctg atctccccgc acgtcaacaa agacgcgcgc
     1981 gatcagtacg aaatccgtac tcacttgcgt ctggttgaca tcgttgagcc aaccgagaaa
     2041 accgttgatg ctctgatgcg tctggatctg gctgccggtg tagacgtgca gatcagcctg
     2101 ggttaatcag gtcattgagc gattgagagg ttgaaacaat gattggttta gtcggtaaaa
     2161 aagtgggtat gacccgtatc ttcacagaag acggcgtttc tatcccagta accgtaatcg
     2221 aagttgaagc aaaccgcgtt actcaggtta aagacctggc taacgatggc taccgtgcta
     2281 ttcaggtgac caccggtgct aaaaaagcta accgtgtgac caagcctgaa gctggccact
     2341 tcgctaaagc tggcgtagaa gctggccgtg gtctgtggga attccgcctg gctgaaggcg
     2401 aagagttcac tgtaggtcag agcattagcg ttgaactgtt tgctgacgtt aaaaaagttg
     2461 acgtaactgg cacctctaaa ggtaaaggtt tcgcaggtac cgttaagcgc tggaacttcc
     2521 gtacccagga cgctactcac ggtaactcct tgtctcaccg cgttccgggt tctatcggtc
     2581 agaaccagac tccgggcaaa gtgttcaaag gcaagaaaat ggcaggtcag atgggtaacg
     2641 aacgtgtaac cgttcagagc cttgacgtag tacgcgttga cgctgagcgc aacctgctgc
     2701 tggttaaagg tgctgtcccg ggtgcaaccg gtagcgacct gatcgttaaa ccagctgtga
     2761 aggcgtaagg agatagcaat ggaattagta ttgaaagacg cgcagagcgc gctgactgtt
     2821 tccgaaacta ccttcggtcg tgatttcaac gaagcgctgg ttcaccaggt tgttgttgct
     2881 tatgcagctg gtgctcgtca gggtactcgt gctcagaaga ctcgtgctga agtaactggt
     2941 tccggtaaaa aaccgtggcg ccagaaaggc accggccgtg cgcgttctgg ttctatcaag
     3001 agcccgatct ggcgttctgg tggcgtgacc tttgctgctc gtccgcagga ccacagtcaa
     3061 aaagttaaca agaagatgta ccgcggcgcg ctgaaaagca tcctgtccga actggtacgt
     3121 caggatcgtc tgatcgttgt cgagaagttc tctgtagaag cgccgaaaac taagctgctg
     3181 gcacagaaac tgaaagacat ggctctggaa gatgtgctga tcatcaccgg tgagctggac
     3241 gaaaacctgt tcctggctgc gcgcaacctg cagaaggttg acgtacgcga tgcaactggt
     3301 atcgacccgg ttagcctgat cgccttcgac aaagtcgtaa tgactgctga tgctgttaag
     3361 caagttgagg agatgctggc atgattcgtg aagaacgtct gctgaaggtg ctgcgtgcac
     3421 cgcacgtttc tgaaaaagcg tctactgcga tggaaaaatc caacaccatc gtactcaaag
     3481 ttgctaaaga cgcgaccaaa gcagaaatca aagctgctgt gcagaaactg tttgaagtcg
     3541 aagtcgaagt cgttaacacc ctggtagtta aagggaaagt taaacgtcac ggacagcgta
     3601 tcggtcgtcg tagcgactgg aaaaaagctt acgtcaccct gaaagaaggc cagaatctgg
     3661 acttcgttgg cggcgctgag taagtcggag gagtaataca atggcagttg ttaaatgtaa
     3721 accgacatct ccgggtcgtc gccacgtagt taaagtggtt aaccctgagc tgcacaaggg
     3781 caaacctttt gctccgttgc tggaaaaaaa cagcaaatcc ggtggtcgta acaacaatgg
     3841 ccgtatcacc actcgtcata tcggtggtgg ccacaagcag gcttaccgta ttgttgactt
     3901 caaacgcaac aaagacggta tcccggcagt tgttgaacgt cttgagtacg atccgaaccg
     3961 ttccgcgaac atcgcgctgg ttctgtacaa agacggtgaa cgccgttaca tcctggcccc
     4021 taaaggcctg aaagctggcg accagattca gtctggcgtt gatgctgcaa tcaaaccagg
     4081 taacaccctg ccgatgcgca acatcccggt tggttctact gttcataacg tagaaatgaa
     4141 accaggtaaa ggcggtcagc tggcacgttc cgctggtact tacgttcaga tcgttgctcg
     4201 tgatggtgct tatgtcaccc tgcgtctgcg ttctggtgaa atgcgtaaag tagaagcaga
     4261 ctgccgtgca actctgggcg aagttggcaa tgctgagcat atgctgcgcg ttctgggtaa
     4321 agcaggtgct gcacgctggc gtggtgttcg tccgaccgtt cgcggtaccg cgatgaaccc
     4381 ggtagaccac ccacatggtg gtggtgaagg tcgtaacttt ggtaagcacc cggtaactcc
     4441 gtggggcgtt cagaccaaag gtaagaagac ccgcagcaac aagcgtactg ataaattcat
     4501 cgtacgtcgc cgtagcaaat aattttagag gataagccat gccacgttct ctcaagaaag
     4561 gtccttttat tgacctgcac ttgctgaaga aggtagagaa agcggtggaa agcggagaca
     4621 agaagcccct gcgcacttgg tcccgtcgtt caacgatctt tcctaacatg atcggtttga
     4681 ccatcgctgt ccataatggt cgtcagcacg ttccggtatt tgtaaccgac gaaatggtcg
     4741 gtcacaaact gggtgaattc gcaccgactc gtacttatcg cggccacgct gctgataaaa
     4801 aagcgaagaa gaaataaggt aggaggaaga gatggaaact attgctaaac atcgccatgc
     4861 tcgttcttct gctcagaagg ttcgccttgt tgctgacctg attcgcggta agaaagtgtc
     4921 gcaggctctg gatattttga cctacaccaa caagaaagcg gctgtacagg tcaagaaagt
     4981 tctggaatct gccattgcta acgctgaaca caacgatggc gctgacattg acgatctgaa
     5041 agttacgaaa attttcgtag acgaaggccc gagcatgaag cgcattatgc cgcgtgcaaa
     5101 aggtcgtgca gatcgcatcc tgaagcgcac cagccacatc actgtggttg tgtccgatcg
     5161 ctgagactct ggagactagc aatgggtcag aaagtacatc ctaatggtat tcgcttgggt
     5221 attgtaaaac catggaactc tacttggttt gcgaacacca aagaattcgc tgacaacctg
     5281 gacagcgatt ttaaagtacg tcagtacctg actaaggaac tggctaaagc gtccgtatct
     5341 cgtatcgtta tcgagcgtcc ggctaagagc atccgtgtaa ccattcacac tgctcgcccg
     5401 ggtatcgtta tcggtaaaaa aggtgaagac gtagaaaaac tgcgtaaggt cgtagcggac
     5461 atcgctggcg ttcctgcaca gatcaacatc gccgaagttc gtaagcctga actggacgca
     5521 aaactggttg ctgacagcat cacttctcag ctggaacgtc gcgttatgtt ccgtcgtgct
     5581 atgaagcgtg ctgtacagaa cgcaatgcgt ctgggcgcta aaggtattaa agttgaagtt
     5641 agcggccgtc tgggcggcgc ggaaatcgca cgtaccgaat ggtaccgcga aggtcgcgta
     5701 ccgctgcaca ctctgcgtgc tgacatcgac tacaacacct ctgaagcgca caccacttac
     5761 ggtgtaatcg gcgttaaagt gtggatcttc aaaggcgaga tcctgggtgg tatggctgct
     5821 gttgaacaac cggaaaaacc ggctgctcag cctaaaaagc agcagcgtaa aggccgtaaa
     5881 taaggagcgt cgctgatgtt acaaccaaag cgtacaaaat tccgtaaaat gcacaaaggc
     5941 cgtaaccgcg gtctggcgca gggtacggat gttagcttcg gcagcttcgg tctgaaagct
     6001 gttggccgtg gtcgtctgac tgcccgtcag atcgaagcag cacgtcgtgc tatgacccgt
     6061 gcagttaagc gtcaaggtaa gatctggatc cgtgtgttcc cggacaaacc gatcactgaa
     6121 aagccgctgg cagtgcgtat gggtaaaggt aaaggtaacg tggagtattg ggttgccttg
     6181 attcagccgg gtaaagtcct gtatgaaatg gacggtgttc cggaagagct ggcccgtgaa
     6241 gcattcaagc tggcagcagc gaaactgccg attaaaacca cctttgtaac taagacggtg
     6301 atgtaatgaa agcaaaagag ctgcgtgaga agagcattga agagctgaac accgagctgc
     6361 tgaacctgct gcgtgagcag ttcaacctgc gtatgcaggc tgcaagtggc cagctgcaac
     6421 agtctcacct gttgaagcaa gtgcgtcgcg atgtcgcacg cgttaagact ttactgaacg
     6481 agaaggcggg tgcgtaatga ccgataaaat ccgtactctg caaggtcgcg ttgttagcga
     6541 caaaatggag aaatccattg ttgttgctat cgaacgtttt gtgaaacacc cgatctacgg
     6601 aaaattcatc aagcgtacga ccaaactgca cgtacatgac gagaacaacg aatgcggtat
     6661 cggtgacgtg gttgaaatcc gcgaatgccg tccgctgtcc aagactaaat cctggacgct
     6721 ggttcgcgtt gtagagaaag cggttctgta atacagtaca ctctctcgat acgaataaac
     6781 ggctcagaaa tgagccgttt attttttcta cccatatcct tgaagcggtg ttataatgcc
     6841 gcgccctcga tatggggatt tttaacgacc tgattttcgg gtctcagtag tagttgacat
     6901 tagcggagca ctaaaatgat ccaagaacag actatgctga acgtcgccga caactccggt
     6961 gcacgtcgcg taatgtgtat caaggttctg ggtggctcgc accgtcgcta cgcaggcgta
     7021 ggcgacatca tcaagatcac catcaaagaa gcaattccgc gtggtaaggt caaaaaaggt
     7081 gatgtgctga aggcggtagt ggtgcgcacc aagaagggtg ttcgtcgccc ggacggttct
     7141 gtcattcgct tcgatggtaa tgcttgtgtt cttctgaaca acaacagcga gcagcctatc
     7201 ggtacgcgta tttttgggcc ggtaactcgt gagcttcgta gtgagaagtt catgaaaatt
     7261 atctctctgg caccagaagt actctaagga gcgaatcatg gcagcgaaaa tccgtcgtga
     7321 tgacgaagtt atcgtgttaa ccggtaaaga taaaggtaaa cgcggtaaag ttaagaatgt
     7381 cctgtcttcc ggcaaggtca ttattgaagg tatcaacctg gttaagaaac atcagaagcc
     7441 ggttccggcc ctgaaccaac cgggtggcat cgttgaaaaa gaagccgcta ttcaggtttc
     7501 caacgtagca atcttcaatg cggcaaccgg caaggctgac cgtgtaggct ttagattcga
     7561 agacggcaaa aaagtccgtt tcttcaagtc taacagcgaa actatcaagt aatttggagt
     7621 agtacgatgg cgaaactgca tgattactac aaagacgaag tagttaaaaa actcatgact
     7681 gagtttaact acaattctgt catgcaagtc cctcgggtcg agaagatcac cctgaacatg
     7741 ggtgttggtg aagcgatcgc tgacaaaaaa ctgctggata acgcagcagc agacctggca
     7801 gcaatctccg gtcaaaaacc gctgatcacc aaagcacgca aatctgttgc aggcttcaaa
     7861 atccgtcagg gctatccgat cggctgtaaa gtaactctgc gtggcgaacg catgtgggag
     7921 ttctttgagc gcctgatcac tattgctgta cctcgtatcc gtgacttccg tggcctgtcc
     7981 gctaagtctt tcgacggtcg tggtaactac agcatgggtg tccgtgagca gatcatcttc
     8041 ccagaaatcg actacgataa agtcgaccgc gttcgtggtt tggatattac cattaccact
     8101 actgcgaaat ctgacgaaga aggccgcgct ctgctggctg cctttgactt cccgttccgc
     8161 aagtaaggta gggttactaa atggctaagc aatcaatgaa agcacgcgaa gtaaaacgcg
     8221 tagctttagc tgataaatac ttcgcgaaac gcgctgaact gaaagcgatc atctctgatg
     8281 tgaacgcttc cgacgaagat cgttggaacg ctgttctcaa gctgcagact ctgccgcgtg
     8341 attccagccc gtctcgtcag cgtaaccgct gccgtcaaac aggtcgtccg catggtttcc
     8401 tgcggaagtt cgggttgagc cgtattaagg tccgtgaagc cgctatgcgc ggtgaaatcc
     8461 cgggtctgaa aaaggctagc tggtaattgt caccaattga atcacgggag taaagacaga
     8521 tgagcatgca agatccgatc gcggatatgc tgacccgtat ccgtaacggt caggccgcga
     8581 acaaagctgc ggtcaccatg ccttcctcca agctgaaagt ggcaatcgcc aacgtgctga
     8641 aggaagaagg ttttattgaa gattttaaag ttgaaggcga caccaagcct gaactggaac
     8701 ttactctgaa gtatttccag ggcaaagctg ttgtagaaag cattcagcgt gtcagccgcc
     8761 caggtctgcg catctataaa cgtaaagatg agctgccgaa agttatggcg ggtctgggta
     8821 tcgcagttgt ttctacctct aaaggtgtta tgactgatcg tgcagcgcgc caggctggtc
     8881 ttggtggcga aattatctgc tacgtagcct aatcggagga aaaaatgtct cgtgttgcta
     8941 aagcaccggt cgttgttcct gccggcgttg acgtaaaaat caacggtcag gttattacga
     9001 tcaaaggtaa aaacggcgag ctgactcgta ctctcaacga tgctgttgaa gttaaacatg
     9061 cagataatac cctgaccttc ggtccgcgtg atggttacgc agacggttgg gcacaggctg
     9121 gtaccgcgcg tgccctgctg aactcaatgg ttatcggtgt taccgaaggc ttcactaaga
     9181 agctgcagct ggttggtgta ggttaccgtg cagcggttaa aggcaatgtg attaacctgt
     9241 ctctgggttt ctctcatcct gttgaccatc agctgcctgc gggtatcact gctgaatgtc
     9301 cgactcagac tgaaatcgtg ctgaaaggcg ctgataagca ggtgatcggc caggttgcag
     9361 cggatctgcg cgcctaccgt cgtcctgagc cttacaaagg caagggggtt cgttacgccg
     9421 acgaagtcgt gcgtaccaaa gaggctaaga agaagtaagg taacactatg gataagaaat
     9481 ctgctcgtat ccgtcgtgcg acccgcgcac gccgcaagct ccaggagctg ggcgcaactc
     9541 gcctggtggt acatcgtacc ccgcgtcaca tttacgcaca ggtaattgca ccgaacggtt
     9601 ctgaagttct ggtagctgct tctactgtag aaaaagctat cgctgaacaa ctgaagtaca
     9661 ccggtaacaa agacgcggct gcagctgtgg gtaaagctgt cgctgaacgc gctctggaaa
     9721 aaggcatcaa agatgtatcc tttgaccgtt ccgggttcca atatcatggt cgtgtccagg
     9781 cactggcaga tgctgcccgt gaagctggcc ttcagttcta aggtagaggt gtaagatggc
     9841 tcacatcgaa aaacaagctg gcgaactgca ggaaaagctg atcgcggtaa accgcgtatc
     9901 taaaaccgtt aaaggtggtc gtattttctc cttcacagct ctgactgtag ttggcgatgg
     9961 taacggtcgc gttggttttg gttacggtaa agcgcgtgaa gttccagcag cgatccagaa
    10021 agcgatggaa aaagcccgtc gcaatatgat taacgtcgcg ctgaataacg gcactctaca
    10081 acaccctgtt aaaggtgttc acacgggttc tcgcgtattc atgcagccgg cttccgaagg
    10141 taccggtatc atcgccggtg gtgcaatgcg cgccgttctg gaagtcgctg gggttcataa
    10201 cgttctggct aaagcatatg gttccaccaa cccgatcaac gtggttcgtg caactattga
    10261 tggcctggaa aatatgaatt ctccagaaat ggtcgctgcc aagcgtggta aatccgttga
    10321 agaaattctg gggaaataaa ccatggcaaa gactattaaa attactcaaa cccgcagtgc
    10381 aatcggtcgt ctgccgaaac acaaggcaac gctgcttggc ttgggtctgc gtcgtattgg
    10441 tcacaccgta gagcgcgagg atactcctgc tattcgcggt atgatcaacg cggtttcctt
    10501 catggttaaa gttgaggagt aagagatgcg tttaaatact ctgtctccgg ccgaaggctc
    10561 caaaaaggcg ggtaaacgcc tgggtcgtgg tatcggttct ggcctcggta aaaccggtgg
    10621 tcgtggtcac aaaggtcaga agtctcgttc tggcggtggc gtacgtcgcg gtttcgaggg
    10681 tggtcagatg cctctgtacc gtcgtctgcc gaaattcggc ttcacttctc gtaaagcagc
    10741 gattacagcc gaagttcgtc tatctgacct ggctaaagta gaaggcggtg ttgtagacct
    10801 gaacacgctg aaagcggcta acattatcgg tatccagatc gagttcgcga aagtgatcct
    10861 ggctggcgaa gtaacgactc cggtaactgt tcgtggcctg cgtgttacta aaggcgctcg
    10921 tgctgctatc gaagctgctg gcggtaaaat cgaggaataa gtagcagatg gctaaacaac
    10981 cgggattaga ttttcaaagt gccaaaggtg gcttaggcga gctgaaacgc agactgctgt
    11041 ttgttatcgg tgcgctgatt gtgttccgta ttggctcttt tattccgatc cctggtattg
    11101 atgccgctgt acttgccaaa ctgcttgagc aacagcgagg caccatcatt gagatgttta
    11161 acatgttctc tggtggtgct ctcagccgtg cttctatctt tgctctgggg atcatgccgt
    11221 atatttcggc gtcgatcatt atccagctgc tgacggtggt tcacccaacg ttggcagaaa
    11281 ttaagaaaga aggggagtct ggtcgtcgta agatcagcca gtacacccgt tacggtactc
    11341 tggtgctggc aatattccag tcgatcggta ttgctaccgg tctgccgaat atgcctggta
    11401 tgcaaggcct ggtgattaac ccgggctttg cattctactt caccgctgtt gtaagtctgg
    11461 tcacaggaac gatgttcctg atgtggttgg gcgaacagat cactgaacga ggtatcggca
    11521 acggtatttc aatcattatc ttcgccggta ttgtcgcggg actcccgcca gccattgccc
    11581 atactatcga gcaagcgcgt caaggcgacc tgcacttcct cgtgttgctg ttggttgcag
    11641 tattagtatt tgcagtgacg ttctttgttg tgtttgttga gcgtggtcaa cgccgcattg
    11701 tggtaaacta cgcgaaacgt cagcaaggtc gtcgtgtcta tgctgcacag agcacacatt
    11761 taccgctgaa agtgaatatg gcgggggtaa tcccggcaat cttcgcttcc agtattattc
    11821 tgttcccggc gaccatcgcg tcatggttcg ggggcggtac tggttggaac tggctgacaa
    11881 caatttcgct gtatttgcag cctgggcaac cgctttatgt gttactctat gcgtctgcaa
    11941 tcatcttctt ctgtttcttc tacacggcgt tggttttcaa cccgcgtgaa acagcagata
    12001 acctgaagaa gtccggtgca tttgtaccag gaattcgtcc gggagagcaa acggcgaagt
    12061 atatcgataa agtaatgacc cgcctgacct tggttggtgc gctgtatatt acctttatct
    12121 gcctgatccc ggagttcatg cgtgatgcaa tgaaagtacc gttctacttc ggtgggacct
    12181 cactgcttat cgttgttgtc gtgattatgg actttatggc tcaagtgcaa actctgatga
    12241 tgtccagtca gtatgagtct gcattgaaga aggcgaacct gaaaggctac ggccgataat
    12301 tggtcgcccg agaagttacg gagagtaaaa atgaaagttc gtgcttccgt caagaaatta
    12361 tgccgtaact gcaaaatcgt taagcgtgat ggtgtcatcc gtgtgatttg cagtgccgag
    12421 ccgaagcata aacagcgcca aggctgattt tttcgcatat ttttcttgca aagttgggtt
    12481 gagctggcta gattagccag ccaatctttt gtatgtctgt acgtttccat ttgagtatcc
    12541 tgaaaacggg cttttcagca tggaacgtac atattaaata gtaggagtgc atagtggccc
    12601 gtatagcagg cattaacatt cctgatcata agcatgccgt aatcgcatta acttcgattt
    12661 atggcgtcgg caagacccgt tctaaagcca tcctggctgc agcgggtatc gctgaagatg
    12721 ttaagatcag tgagctgtct gaaggacaaa tcgacacgct gcgtgacgaa gttgccaaat
    12781 ttgtcgttga aggtgatctg cgccgtgaaa tcagcatgag catcaagcgc ctgatggatc
    12841 ttggttgcta tcgcggtttg cgtcatcgtc gtggtctccc ggttcgcggt cagcgtacca
    12901 agaccaacgc acgtacccgt aagggtccgc gcaaaccgat caagaaataa tcggggtgat
    12961 tgaataatgg caaaggcacc aattcgtgca cgtaaacgtg taagaaaaca agtctctgac
    13021 ggcgtggctc atatccatgc ttctttcaac aacaccatcg tgactatcac tgatcgtcag
    13081 ggtaacgcgt tgggttgggc aacagccggt ggttccggtt tccgtggttc tcgcaaatcc
    13141 actccgtttg cagctcaggt tgcagcagag cgttgcgctg acgccgtgaa agaatacggc
    13201 atcaagaatc tggaagttat ggttaaaggt ccgggtccag gccgcgaatc tactattcgt
    13261 gctctgaacg ccgcaggttt ccgcatcact aacattactg atgtgactcc gatccctcat
    13321 aacggttgtc gtccgccgaa aaaacgtcgc gtataacgcc tcgttttcca ggtttgttgg
    13381 agaaagaaaa tggcaagata tttgggtcct aagctcaagc tgagccgtcg tgagggcacc
    13441 gacttattcc ttaagtctgg cgttcgcgcg atcgatacca agtgtaaaat tgaacaagct
    13501 cctggccagc acggtgcgcg taaaccgcgt ctgtctgact atggtgtgca gttgcgtgaa
    13561 aagcaaaaag ttcgccgtat ctatggtgtg ctggagcgtc agttccgtaa ctactacaaa
    13621 gaagcagcac gtctgaaagg caacaccggt gaaaacctgt tggctctgct ggaaggtcgt
    13681 ctggacaacg ttgtataccg tatgggcttc ggtgccactc gtgcagaagc acgtcagctg
    13741 gttagccata aagcaattat ggtaaacggt cgtgttgtta acatcgcttc ttatcaggtt
    13801 agtccgaatg acgttgtaag cattcgtgag aaagcgaaga agcagtctcg cgtgaaagcc
    13861 gctctggagc tggctgagca gcgtgaaaag ccaacttggc tggaagttga tgctggcaag
    13921 atggaaggta cgtttaagcg taagccggag cgttctgatc tgtctgcgga cattaacgaa
    13981 cacctgatcg tcgagcttta ctccaagtaa agcttagtac caaagagagg acacaatgca
    14041 gggttctgtg acagagtttc taaaaccgcg cctggttgat atcgagcaag tgagttcgac
    14101 gcacgccaag gtgacccttg agcctttaga gcgtggcttt ggccatactc tgggtaacgc
    14161 actgcgccgt attctgctct catcgatgcc gggttgcgcg gtgaccgagg ttgagattga
    14221 tggtgtacta catgagtaca gcaccaaaga aggcgttcag gaagatatcc tggaaatcct
    14281 gctcaacctg aaagggctgg cggtgagagt tcagggcaaa gatgaagtta ttcttacctt
    14341 gaataaatct ggcattggcc ctgtgactgc agccgatatc acccacgacg gtgatgttga
    14401 aatcgtcaag ccgcagcacg tgatctgcca cctgaccgat gagaacgcgt ctattagcat
    14461 gcgtatcaaa gttcagcgcg gtcgtggtta tgtgccggct tctacccgaa ttcattcgga
    14521 agaagatgag cgcccaatcg gccgtctgct ggtcgacgca tgctacagcc ctgtggagcg
    14581 tattgcctac aatgttgaag cagcgcgtgt agaacagcgt accgacctgg acaagctggt
    14641 catcgaaatg gaaaccaacg gcacaatcga tcctgaagag gcgattcgtc gtgcggcaac
    14701 cattctggct gaacaactgg aagctttcgt tgacttacgt gatgtacgtc agcctgaagt
    14761 gaaagaagag aaaccagagt tcgatccgat cctgctgcgc cctgttgacg atctggaatt
    14821 gactgtccgc tctgctaact gccttaaagc agaagctatc cactatatcg gtgatctggt
    14881 acagcgtacc gaggttgagc tccttaaaac gcctaacctt ggtaaaaaat ctcttactga
    14941 gattaaagac gtgctggctt cccgtggact gtctctgggc atgcgcttgg aaaactggcc
    15001 accggcaagc atcgctgacg agtaaccgga tcacaggtta aggttttact gagaaggata
    15061 aggtcatgcg ccatcgtaag agtggtcgtc aactgaaccg caacagcagc catcgccagg
    15121 ctatgttccg caatatggca ggttcactgg ttcgtcatga aatcatcaag acgactctgc
    15181 ctaaagcgaa agagctgcgc cgcgtagttg agccgctgat tactcttgcc aagactgata
    15241 gcgttgctaa tcgtcgtctg gcattcgccc gtactcgtga taacgagatc gtggcaaaac
    15301 tgtttaacga actgggcccg cgtttcgcga gccgtgccgg tggttacact cgtattctga
    15361 agtgtggctt ccgtgcaggc gacaacgcgc cgatggctta catcgagctg gttgatcgtt
    15421 cagagaaagc agaagctgct gcagagtaat ctgaagcaat gtaaaaaaac ccgccccggc
    15481 gggttttttt atacccgtag tatccccact tatctacaat agctgtactc tttt
//
LOCUS       DADLTY010000097        15483 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.097, whole genome shotgun sequence.
ACCESSION   DADLTY010000097 DADLTY010000000
VERSION     DADLTY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.097"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..573)
                     /gene="ppiA"
                     /locus_tag="JN334_002601"
                     /gene_synonym="rotA"
     CDS             complement(<1..573)
                     /gene="ppiA"
                     /locus_tag="JN334_002601"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HAY7438701.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKV"
     gene            844..2025
                     /gene="tsgA"
                     /locus_tag="JN334_002602"
     CDS             844..2025
                     /gene="tsgA"
                     /locus_tag="JN334_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HAY7438702.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            2287..4830
                     /locus_tag="JN334_002603"
     CDS             2287..4830
                     /locus_tag="JN334_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HAY7438703.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            4827..5153
                     /gene="nirD"
                     /locus_tag="JN334_002604"
     CDS             4827..5153
                     /gene="nirD"
                     /locus_tag="JN334_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HAY7438704.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            5279..6085
                     /gene="nirC"
                     /locus_tag="JN334_002605"
     CDS             5279..6085
                     /gene="nirC"
                     /locus_tag="JN334_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAY7438705.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            6104..7477
                     /gene="cobA"
                     /locus_tag="JN334_002606"
     CDS             6104..7477
                     /gene="cobA"
                     /locus_tag="JN334_002606"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY7438706.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            7724..7891
                     /locus_tag="JN334_002607"
     CDS             7724..7891
                     /locus_tag="JN334_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HAY7438707.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            8186..9523
                     /gene="frlA"
                     /locus_tag="JN334_002608"
                     /pseudo
     CDS             8186..9523
                     /gene="frlA"
                     /locus_tag="JN334_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            9544..10566
                     /gene="frlB"
                     /locus_tag="JN334_002609"
     CDS             9544..10566
                     /gene="frlB"
                     /locus_tag="JN334_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HAY7438708.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            10616..11446
                     /gene="frlC"
                     /locus_tag="JN334_002610"
     CDS             10616..11446
                     /gene="frlC"
                     /locus_tag="JN334_002610"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HAY7438709.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            11443..12228
                     /gene="frlD"
                     /locus_tag="JN334_002611"
     CDS             11443..12228
                     /gene="frlD"
                     /locus_tag="JN334_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAY7438710.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            12328..13059
                     /gene="phoB"
                     /locus_tag="JN334_002612"
     CDS             12328..13059
                     /gene="phoB"
                     /locus_tag="JN334_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HAY7438711.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(13219..14292)
                     /locus_tag="JN334_002613"
     CDS             complement(13219..14292)
                     /locus_tag="JN334_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY7438712.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLATLRAAG
                     VPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRATLYS
                     GGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANAQSKV
                     LIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLEHCAI
                     RINPNRSGEETVLRILRESIASI"
     gene            complement(14304..>15483)
                     /locus_tag="JN334_002614"
     CDS             complement(14304..>15483)
                     /locus_tag="JN334_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438713.1"
                     /translation="YMNRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDI
                     LGVLAINSLMAFGLGAIWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALF
                     PLVAIFYQFGWKQSLVAAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDL
                     RHRDENDIDASGLSVFEERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEK
                     AYSAGVTPEQSQTLINQAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLS
                     PNPMVAAVLGAVVISAEVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSI
                     FAAIKMAGYTGFSIAVAIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA
                     "
BASE COUNT         3656 a         4108 c         4044 g         3675 t
ORIGIN      
        1 gactttagcg gaaaggataa ctaccggttt tgacggcaca ttctggtacg gaccaacatc
       61 atgagtcggc acctgggaaa tcttatcggc aacgtccatg cctttcacca ctttaccaaa
      121 taccgcgtaa ccgaaatcac gctgaccatg gtcaaggaag gcgttatcgg caacgttgat
      181 aaagaactgg ctggtggcgc tgtctttgtc agcggtacgc gccatcgcga tggtgccacg
      241 cgtgttgcgc aggccgttat cggcttcatt tttgattggc gggtttggtt ttttctgctg
      301 catctgctcg gtgaaaccgc cgccctgaat cataaagcca ggaatgacgc ggtgaaaggt
      361 agtgttgtta taaaaaccgc tgttcacata atcgacaaag ttttgcacag acactggcgc
      421 tttttgttta tccagctcca gttcgatgtt accagctgag gttgtcaaca atacgtgcgg
      481 gtcccctttc gctgccattg ctgcgggaga aagagcagaa agagcagaaa gagcgaaaac
      541 agcagccatc gccgccaggg tcgatttgaa catgagattt ccttaacaaa cagagcagaa
      601 aaaaagcaag tgaagagatt ctaaatagcc ttcaagaaca aggccatccc tttacctaat
      661 tttacgtatc tgaaatatat gtaacctgac gatcactttt tattccgtga tcaaaatcac
      721 ctcttaaaat gcaatttagc aaccgattgc aataaaacat ttaaacagat cataaaatca
      781 cctaaaatcg cccgctcgcg gcatatcccg ctatgccatt ttatttttga acgcaggcca
      841 atcatgacta acagcaatcg catcaagctc acatggatta gctttctctc ctacgcactg
      901 actggtgcgt tggttattgt caccgggatg gtgatgggaa atatcgccga ttatttcaat
      961 ctgcctgttt ccagtatgag taacaccttc accttcctca acgccggcat tttaatctct
     1021 atcttcctca acgcctggct gatggaaatc gtcccgttga aaacgcagtt acgttttggc
     1081 tttctcctga tggtgctggc ggttgccggt ttgatgttca gccacagcct ggcgctgttc
     1141 tcgacggcga tgttcattct cggggtggtc agcggcatta ctatgtcgat tggtacattc
     1201 ctgataacac aaatgtatga agggcgtcag cgcggttccc gcctgttatt tactgactcc
     1261 ttcttcagta tggctgggat gattttccca atgatcgccg cgtttctact ggcgcgcagc
     1321 attgagtggt actgggttta tgcctgcatc gggctggtgt atgtcgctat ttttattctg
     1381 accttcggct gtgagttccc ggcactgggc aaacgtgcac caaaaacgga tgctccggta
     1441 gagaaagaaa agtgggggat cggcgtactg tttctctctg ttgcggcgct gtgctacatc
     1501 ctcggtcagt taggttttat ctcctgggtg cctgagtatg ccaaaggcct gggcatgagc
     1561 ctgaacgacg cgggcacgct ggtgagtaac ttctggatgt catacatggt cggcatgtgg
     1621 gcgttcagct ttattcttcg cttctttgat ttgcaacgca ttctgaccgt actggctggt
     1681 ctggctgcga ttctgatgta cgtctttaac accggaacac cagtacatat ggcgtggtca
     1741 attctcgctc tgggcttctt ctccagcgcg atctatacca ccatcatcac cctgggttca
     1801 cagcagacca aagtaccgtc gccaaaactg gttaactttg tcctgacctg cggaaccatc
     1861 ggtactatgt tgacctttgt ggttaccggc acgattgttg aacatagcgg tccgcaggcg
     1921 gcactgctta cagcaaacgg tctgtacgct gtcgtctttg tgatgtgctt cctgttaggt
     1981 ttcgtcagcc gtcaccgtca gcataacacc ctgacctctc attaattgct aatgccggac
     2041 ggcactatcg tcgtccggcc ttttcctctc ttcccccgct acgtgcatct atttctataa
     2101 acccgttcat tttgtccatt ttttgcacaa acatgaaatg tcagacaatt ccgtgactta
     2161 agaaaattta tacaaatcag caatataccc cttaaggagt atataaaggt gaatttgatt
     2221 tacatcaata agcggggttg ctgaatcgtt aaggtaggcg gtaatagaaa agaaatcgag
     2281 gcaaaaatga gcaaagtcag actcgcaatt atcggtaacg gtatggtcgg ccatcgcttt
     2341 atcgaagatc ttcttgataa atctgatgcg gccaactttg atattaccgt tttctgtgaa
     2401 gaaccgcgca tcgcttatga ccgcgtacac ctctcatctt acttctctca ccacaccgcc
     2461 gaagagctgt cgctggtgcg cgaaggcttc tacgagaaac acggcatcaa agttctggtc
     2521 ggcgaacgcg ctatcaccat caaccgtcag gagaaggtga ttcactccag cgccggacgt
     2581 accgtttttt atgacaagct gatcatggca accggttcct acccgtggat cccaccaatc
     2641 aaaggttctg atactcagga ctgctttgtc tatcgcacca ttgaagacct caacgccatt
     2701 gaatcctgcg cccgtcgcag caaacgcggt gccgttgttg gtggcggcct gttaggtctg
     2761 gaagccgctg gcgcgctgaa aaacttaggt attgaaaccc acgttatcga atttgcccct
     2821 atgctgatgg cggaacagct tgatcagatg ggtggcgagc agctgcgtcg caaaatcgaa
     2881 agtatgggcg tgcgcgttca caccagcaaa aacacccttg agattgtgca ggaaggtgtc
     2941 gaagcgcgta aaaccatgcg ttttgccgac ggcagcgaac tggaagtcga ctttatcgtc
     3001 ttctctaccg gtatccgtcc gcgcgataag ctggcaaccc agtgtggtct ggacgttgct
     3061 ccgcgtgggg ggattgtcat taatgattcc tgccagactt ccgatccgga tatctacgcc
     3121 atcggtgaat gcgcaagctg gaacaaccgt gtatttggtc tggtagcacc aggctacaaa
     3181 atggcgcagg tcgccgttga ccatattctg ggtagcgaaa acgcctttga aggtgctgac
     3241 cttagcgcca agctgaaact gctgggcgta gacgtaggtg gtattggtga tgcgcacggt
     3301 cgcacgcctg gcgcacgtag ctatgtttac ctcgacgaaa gtaaagagat ctacaaacgc
     3361 ctgattgtca gcgaagacaa caaaaccctg ctcggtgcgg tactggtggg cgataccagc
     3421 gactacggca acctgctgca actggtgctg aacgctatcg agctgccgga aaacccggat
     3481 tctctgattc tgccggcgca ctcgggtagc ggtaagccgt ctatcggtgt tgataaactg
     3541 ccggacagcg cgcaaatctg ctcctgcttc gacgtcacta aaggtgatct gattgctgcc
     3601 atcaacaaag gctgccacac agttgcggcg ctgaaagctg aaaccaaagc gggtactggc
     3661 tgcggtggct gtatcccgct ggtcactcag gtactgaacg cggaactggc gaaacagggc
     3721 atcgaagtta acaacaacct gtgcgaacac tttgcttatt cgcgtcagga actgttccat
     3781 ttgatccgcg ttgaaggcat taaaaccttc gaagaactgc tggcgaaaca cggcaaaggc
     3841 tacggttgtg aagtttgtaa accaaccgtc ggttcgctgc tggcctcctg ctggaacgaa
     3901 tacattctga agccggaaca tactccgctg caggattcta acgacaactt cctcgctaac
     3961 atccagaaag acggcaccta ctcggtgatc ccgcgttctc cgggcggtga aatcaccccg
     4021 gaagggctga tggcagtggg tcgtatcgcg cgtgaattta atctctacac caaaatcacc
     4081 ggctcccagc gcctggcgat gtttggcgca cagaaagacg atctgccgga aatctggcgt
     4141 cagctgattg aagctggctt cgaaaccggt catgcctatg cgaaagcact gcgtatggcg
     4201 aaaacctgcg tgggtagcac ctggtgccgc tacggtgttg gcgatagcgt cggcctcggc
     4261 gtggaactgg aaaaccgcta caaaggcatc cgtacgccgc acaaaatgaa gttcggtgtc
     4321 tccggctgta cccgtgaatg ttcagaagct cagggtaaag acgtgggtat tatcgccact
     4381 gaaaaaggct ggaacctgta tgtttgcggt aacggcggca tgaaaccgcg tcatgcggat
     4441 ctgctggcag cggatatcga tcgcgaaacg ctgatcaaat atctcgaccg cttcatgatg
     4501 ttctacatcc gtaccgccga caaactgacg cgtaccgcgc cgtggttaga aaacctcgaa
     4561 ggcggcatcg attacctgaa agcggtgatc attgacgaca aactggggct gaacgctcat
     4621 ctggaagaag agatggcgcg cctgcgtgaa gcggtagtgt gtgagtggac tgaaacggtc
     4681 aatacaccgt ccgcgcagac tcgcttcaaa cacttcatca acagcgacaa gcgtgacccg
     4741 aacgtgcaga tggtgccaga gcgcgaacag caccgtccgg caacgccgta tgaacgtatc
     4801 ccggtaactc tggtggagga caacgcatga gccagtggaa agacatctgc aaaatcgatg
     4861 acatcctgcc tgaaaccggc gtctgcgcac tgttaggtga caagcaggta gcgattttcc
     4921 gcccgtatca cagcgaccag gtgtttgcga tcagcaacat tgatccgttc ttcgagtcca
     4981 gcgtgctatc acgcgggctg attgcggaac accagggcga gctgtgggtc gccagcccgc
     5041 tgaaaaaaca gcgttttcgc ttaagtgatg gcttgtgcat ggaagacgaa cagttttccg
     5101 tcaaacatta cgaagcacga gtgaaagacg gcgtggtgca gctgcgcggt taatgtttta
     5161 acgggaggcg caatgcctcc cctttttgca tggtcctgta atgaccttcg gtatattgca
     5221 ggacattttt taaacttttt gttttatttt ttgtttttat tttttaaagg ataatcaaat
     5281 gtttacagac actattaata agtgtgcggc taatgctgcg cgcattgcac gcctgtcggc
     5341 aaataacccg ctcggctttt gggtcagctc cgccatggcg ggcgcatatg tgggtcttgg
     5401 gatcatcctg attttcacgc tcggtaattt gctcgacccg tccgtacgtc cgctggtgat
     5461 gggcgcgacc tttggtatcg ccttaacgct ggtgattatc gccggttctg aactgttcac
     5521 cggacacacc atgttcctca cctttggggt aaaagcgggc agcatcagcc acgggcaaat
     5581 gtgggcaatc ctgccgcaaa cctggctggg taacctggtc ggttccgtct tcgttgccat
     5641 gctctatagc tggggcggcg gtagcctgct gccggtagat accagcatcg ttcactccgt
     5701 cgcgctggct aaaaccactg caccggcaat ggtactgttc ttcaaaggtg cattgtgtaa
     5761 ctggctggtt tgcctggcaa tctggatggc gctgcgcact gaaggggcgg cgaaatttat
     5821 cgctatctgg tggtgtctgc tggcatttat cgcgtccggc tacgagcact ctatcgctaa
     5881 catgacgctg ttcgcgctct cctggttcgg caaccacagc gaagcctaca cgctggcggg
     5941 tattggtcat aacctgctgt gggtgacgct gggtaatact ttatcaggtg ccgtattcat
     6001 gggattgggt tattggtatg ctacgccgaa agcgaatcgt ccggttgcgg acaaatttaa
     6061 tcaaactgaa acagctgccg gttaattact aaggggtttt tacgtggatc atttgcctat
     6121 attttgccaa ttacgcgatc gcgactgtct gattgtcggc ggtggtgatg tcgcggaacg
     6181 caaagcaagg ttgctgttag acgcaggcgc tcgcttaacg gtgaatgcat tagcgtttat
     6241 tccacagttc accgcatggg cagatgcagg catgttaacc ctcgtcgaag ggccatttga
     6301 tgaaagcctt ctcgacacct gctggctggc gattgcagcg acggatgatg acacgcttaa
     6361 ccagcgcgtc agcgaagccg ctgaagctcg tcgcatcttc tgtaacgtag ttgatgcgcc
     6421 gaaagccgcc agctttatta tgccgtcgat tattgaccgc tcaccgctaa tggtagcggt
     6481 ctcctctggc ggcacctctc cggttctggc gcgcctgttg cgcgaaaaac tcgaatcact
     6541 gctgccgttg catctgggcc aggtggcgaa atatgccggg caattacgcg ggcgagtgaa
     6601 acaacagttc gccacgatga gtgagcgtcg ccgtttctgg gagaaattgt tcgttaacga
     6661 ccgcctggcg cagtcgctgg caaacaacga tcagaaagcc attactgaaa cgaccgaaca
     6721 gttaatcaac gaaccgctcg accatcgcgg tgaagtggtg ctggttggtg caggtccggg
     6781 cgatgccggg ctgctgacgc tgaaaggact gcaacaaatt cagcaggcag atgtggtggt
     6841 ctacgaccgt ctggtttctg acgatattat gaatctggta cgccgcgatg ctgatcgcgt
     6901 tttcgtcggc aaacgcgcgg gataccactg tgtaccgcag gaagagatta accagatcct
     6961 gctgcgggaa gcgcaaaaag gcaaacgcgt ggtgcggctg aaaggtggcg atccgtttat
     7021 ttttggccgt ggtggcgaag agctggaaac actgtgcaac gcgggtattc cgttctcggt
     7081 ggttccgggt attaccgcag cttctggttg ctctgcctat tcgggtattc cactcacgca
     7141 tcgcgattat gcccagagcg tacgcttaat taccggacac ttaaaaaccg gtggcgagct
     7201 ggactgggaa aacctggcgg cagaaaaaca gacgctggtg ttctatatgg ggttgaatca
     7261 ggccgcgact attcagcaaa agctgattga acacggtatg cctggcgaaa tgccggtggc
     7321 aattgtcgaa aacggaacgg cagtcacgca gcgcgtgatt gacggtacgc tcacgcaact
     7381 cggtgaactt gctcagcaaa tgaacagtcc atcgctaatt attattggtc gggttgttgg
     7441 cctgcgcgat aaactgaact ggttctccaa tcattaattt aacccgggcc agagaattct
     7501 ggccttctta atgactcttt ttatatattc agcaaataaa acataacccc ttaataaata
     7561 ttatctgcta atgggttata ttcaccgtta cagcgattta caaaattaag tcattcgctc
     7621 actgacgcca attaaatatt cctgtatcgt tcttaacgag cagtctgctt atattcatat
     7681 aatcaatgaa tattaattaa tatataatac atagggaatg taaatgaaca aatttattaa
     7741 agttgcactg gtaggtgcag tactggctac gttaactgca tgtactggtc atattgaaaa
     7801 ccgtgataag aactgctctt acgactacct gctgcacccg gcaatttcta tttctaaaat
     7861 cattggcggt tgcggtccta ctgcacagta agtctctgac agaaatcggc taacaccggt
     7921 ttaattgccc gacgccctct ggattttccg agggcgtatt tgtttctatg tccttcttgc
     7981 ccccgcccgt aaaaatattt tcactttaaa ttcaatttta taactacatc cattagccac
     8041 agcgcgatcc tgtgcgaaat tttgtgatct tcctccacat cacacaacat aatatgttgt
     8101 tatattcatc atgcattgtc atgttacctt ttaaatggct gcaaactctc ccctacaacg
     8161 tattggacaa gaaaaaggta tcgctatggg aagccaggaa ctccaacgca agctcggatt
     8221 ttgggccgtt cttgcaatcg ccgtcgggac aaccgtcggc tccggtattt ttgtatctgt
     8281 gggtgaagtg gcaaaagcag cgggcacgcc gtggcttacg gtgctcgcgt ttgtcattgg
     8341 cgggttaatt gtgatcccgc aaatgtgcgt ctatgcggaa ctatccaccg cttatccgga
     8401 aaatggcgca gattatgttt atctgaaaaa tgccggaagc cgaccgctgg ctttcctctc
     8461 cggctgggcc agcttctggg ccaacgatgc gccgtcattg tcgattatgg cgctggcgat
     8521 tgtcagcaat cttggctttt taacgcctat cgatccgttg ctcggtaaat ttatcgccgc
     8581 cggattaatt atcgccttta tgttgctaca cctgcgctcc gttgaaggcg gcgcagcgtt
     8641 tcagacgcta attaccatcg ccaaaattat cccgttcact atcgtcattg gccttgggat
     8701 cttctggttt aaagcggaga attttgccgc ccttaccacc actgcgattg gcgcaacggg
     8761 cagctttatg gcgctgctgg cggggatctc tgccaccagt tggtcgtata ccggcatggc
     8821 ctctatctgt tatatgaccg gcgaaattaa aaaccccgga aaaaccatgc cacgagcgct
     8881 gattggttcc tgtctgctgg ttctggtgct ctacaccctg ctggcgctgg tgatttccgg
     8941 cctgatgccc ttcgacaaac tcgccaattc tgaaacgccg atttccgacg ccctgaccta
     9001 gatccccgca ctcggcagca ccgctgggct ctttgttgcc atcacggcga tgatcgtcat
     9061 tcttggttcg ctttccagct gcgtgatgta ccagccgcgg ctggaatacg cgatggcgaa
     9121 agacaacctg ttctttaaat gcttcggcca tgtgcatccg aaatacaaca cgccggatgt
     9181 ctccatcatc ctgcaagggg cgctggggat cttcttcatc ttcgtttccg atctcaccag
     9241 cctgctgggt tatttcaccc tggtgatgtg tttcaaaaat accctcacct tcggctccat
     9301 catctggtgt cgtaaacgcg acgattacaa accgctgtgg cgtactccgg ctttcgggct
     9361 gatgaccacc ctcgccattg cgtcaagcct cattctggtc gcctcaacct ttgtctgggc
     9421 accgattccc ggccttatct gcgccgccat cgttattgct actggtctgc ctgcttacgc
     9481 cttctgggcg aagcgtagcc gccagctcaa cgctttgtcg taatgttatc tgaagaaaat
     9541 aaaatgttgg atattgataa aagcaccgtg gactttctgg tcaccgaaaa tatggttcag
     9601 gaagtggaaa aagttctcag ccatgacgtt ccgctggtgc acgccatcgt ggaagagatg
     9661 gtgaagcgcg acattgatcg tatttatttc gttgcctgcg gctcgccact caacgcggcg
     9721 caaacggcga aacatctggc ggatcgcttt tccgatcttc aggtctacgc catttccggc
     9781 tgggagttct gcgataacac cccgtatcgc ctcgacgatc gttgcgcggt gattggcgtt
     9841 tctgactacg gtaaaacgga agaggtaatc aaagcgctgg agctgggccg ggcctgcgga
     9901 gcactcactg cggcgttcac caaacgcgcg gacagcccga ttacctcggc ggcggaattt
     9961 agcattgatt atcaggccga ctgtatctgg gaaattcacc tgctgctctg ctacagcgtg
    10021 gtgctggaga tgatcacccg cctcgcgccg aacgcggaaa tcggcaagat caaaaacgat
    10081 ctcaagcagt tgccgaatgc gctcggtcat ctggtacgca cctgggaaga aaaaggccgc
    10141 cagcttggtg aactggccag ccagtggccg atgatttata ccgttgctgc gggtccgctg
    10201 cgtccgctgg gttacaaaga aggcattgta acgctgatgg aatttacctg gacgcacggc
    10261 tgcgtgattg agagcggaga gttccgccat ggcccgctgg agattgtcga accgggcgtt
    10321 ccgttcctgt tcctgctcgg caatgatgaa agtcgccaca ccaccgaacg cgccattaac
    10381 tttgttaaac agcgtactga caacgtgatc gtcatcgatt acgccgaaat ttcgcaaggg
    10441 ctgcacccgt ggctggcacc gttcctgatg ttcgtgccaa tggagtggct ctgctactac
    10501 ctgtctattt acaaagatca caacccggat gaacgccgct attacggtgg tctggtggaa
    10561 tattaatccc tctcccggcc cggcaacggg ccggatttac gcaaggagtt acccgatgaa
    10621 aacaggtatg tttacctgcg gccaccagcg gctgcctatt gaacacgcat ttcgtgatgc
    10681 aagcgagctg ggttacgacg gcatcgaaat ttggggcggt cgcccgcacg cgttcgcgcc
    10741 ggacttaaaa gcgggcggca tcaaacaaat caaggcgctg gcgcagacgt atcagatgcc
    10801 gattatcggt tacacgccag aaaccaacgg ctatccgtat aacatgatgc tgggcgatga
    10861 acatatgcgt cgtgaaagcc tggacatgat caagctggcg atagatatgg caaaagagat
    10921 gaacgcgggt tatacgctga tttctgcggc ccacgcgggc tatctcacgc cgcctaacgt
    10981 tatctggggg cggctggcag agaacttaag cgaactgtgt gagtacgcgg aaaacatcgg
    11041 catggatctg atcctcgaac cgttaacgcc gtatgaatcg aacgtcgtgt gtaatgctaa
    11101 tgatgtgctt catgcgctgg cgctggtgcc ttcgccgcgc ctgttcagca tggtcgacat
    11161 ttgcgcgccg tatgtccagg cggaaccggt gatgagttat ttcgacaaac tgggcgataa
    11221 attacgtcat ctgcatattg tcgacagcga cggggccagc gacacgcatt acattcctgg
    11281 agaaggcaaa atgccgctgc gggaactgat gcgcgatatt attgatcggg gctatgaggg
    11341 ttactctacg gtggagctgg tgacgatgta tatgaacgag cccagactct atgcccgcca
    11401 ggcgctggaa cgctttcgcg cgctgctgcc ggaggatgag agatgaaaac cctggcgaca
    11461 atcggcgata actgcgtcga tatctacccg caactgaata aagcgttttc tggcggtaat
    11521 gcggtcaatg tggcggtgta ctgcactcgc tacggcatac agccgggatg cattacctgg
    11581 gtgggtgacg atgactacgg cacaaagctg aagcaggatc tcgcccgcat gggcgtcgat
    11641 atcagccatg tccatacgaa acacggcgtt accgcacaaa ctcaggtgga actgcacgac
    11701 aatgatcgcg tttttggcga ctacaccgaa ggcgtgatgg ccgactttgc cctgagtgaa
    11761 gaggattacg cctggctggc gcagtatgac attgtgcacg cggcaatctg gggacatgcg
    11821 gaagacgcat tcccacagct gcacgctgcg ggcaaactta ccgctttcga cttctccgac
    11881 aagtgggaca gcccgctctg gcagacactg gtgccgcatc tcgattttgc ctttgcctcc
    11941 gcaccgcaag aagacgaagc gctgcgtctg aagatgaaag cgattgttgc ccgtggcgca
    12001 ggaacagtga ttgtcacgct gggtgaaaac ggcagcattg cctgggatgg cgcgcagttc
    12061 tggcgtcagg ctcctgaacc ggtgacggtt atcgacacca tgggtgccgg agattcgttc
    12121 attgccggat tcctttgcgg ctggtctgcg gggatgacat taccgcaggc gatggcgcag
    12181 ggaacggcgt gcgcggcgaa aaccattcag taccacggtg cctggtaggt ataacgttgg
    12241 cgtgagcatc ttcacgccaa cgtgctgtta cttgccggaa aacgacccta taatccgagt
    12301 aattcattct ttatttcagg gtcgattatg tcagctacgg accgctactc tcatcaactc
    12361 ctctacgcta ccgtccgcca gcggctgctg gatgatatcg cgcagggggt ttaccaggcc
    12421 gggcaacaga tccctaccga aaacgagctt tgtacacaat ataacgtcag ccgcattacc
    12481 attcgcaaag ccatcagcga cttagtggca gacggcgtac tgatccgctg gcagggaaaa
    12541 ggcacctttg tacaaagcca gaaagttgaa aacgccctgc ttactgtcag tggttttacc
    12601 gattttggcg tctcacaagg caagtcgacg aaagagaaag tgatcgaaca ggaacgggtc
    12661 agcgccgcgc cgttttgcga aaagctgaac atccccggaa acagcgaagt gttccatctc
    12721 tgccgggtga tgtatctcga taaagagccg ctgtttattg atagttcatg gatcccgctg
    12781 tcgcgttatc ctgactttga tgagatttac gtcgaaggaa gctccaccta tcagttattt
    12841 caggagcgtt ttgacacgcg agtggtcagc gacaaaaaga ccatcgatat ctttgccgcc
    12901 acccgcccgc aggcaaaatg gctgaaatgc gaactgggcg aaccgttgtt tcgcatcagc
    12961 aaaatcgcct ttgatcagaa tgacaaaccg gtgcacgtct ccgaactctt ctgccgcgcc
    13021 aatcgcatca ccttaacgat tgataataaa agacattaac cgtaggccgg ataagatgcg
    13081 ccagcatcgc atccggcgat gctggcgcac tgaattttac atcccgcacg ttcccctcac
    13141 cctaaccctc tccccaaagg ggcgagggga ccgttcagtg cggaatttag cttgattgcc
    13201 gaacggtgct atccagcatt aaatactcgc aatactctca cgcaaaatcc gcagcaccgt
    13261 ctcttcaccg ctgcgattcg ggttaatgcg aatcgcacaa tgttcgagtt gtggattcgc
    13321 ctggcgaaac gttcctgaaa gtcgatagaa gagcggcggg atttcatatt tcgactctgc
    13381 accgaccggg taaggcaacg cgccacgctt ttgcgcttct tccagcaccc tggcggcaat
    13441 cagctgatga aactcgacaa tcaacacctt cgactgcgca ttggcaatga cggcgctttt
    13501 cacttccgcc accgcgccgc cgttaagcaa agccagcaac cgttcggata ctcccgcctg
    13561 caccgcatgc atcactggcg cgaacaccag accacgcaag acttccagcg cctgtgagcc
    13621 ctggatctgg ctaccgccgg agtaaagcgt ggcgcgaatc cgactaataa catcggcatc
    13681 gccaaccact gcaccaacgc cctccggccc aaacagcttg aagcaggaga atgtcgagac
    13741 attcgcgccg cattcacagc cgattcgcgc taccttcatc accgcatagt tatcatcggt
    13801 taacgctgga acacctgccg cgcgcaacgt tgccagcaca tagctgtcct gcggctgctg
    13861 gcgcgtatgc tgcaccagcg cggcatcagg ttgttgctcg tcgacgacct gcttcagtgc
    13921 cgacaggtca ttgaaatcag cagtaataag cgtcagcccc atctgctcaa taataacccg
    13981 tgtcgtcggg taaacaggcg cgtcatgcac cagaagacgc tgccccggtt tgagcaaagc
    14041 cgccagcccg gcgcgaatcg cgccagtccc cgcgccctgc accagcgccg cggcctgtgc
    14101 gtgaaatgca tcagccagca cctgctccac ccgttgggta atacgcggtt gattcagact
    14161 tggagttaac cctaaatcac cgccagcaag aaactcgctg cctggaaaat ggcggcaaat
    14221 agtgtccacc agcgcaaact gcttttgctg cgcttcagca agagtcaggc tttgcaaagg
    14281 aaacgtcttc ataggtgcct tccttaagcc ggaacgaaca ggccaagcca gtaaagaaca
    14341 ttcagcagaa taccggtgat cattactgcc acgaccggtg ccgccatttt ctgtaccgga
    14401 cggcccaggg attcgttgag gaagtaaatg gcaaccgcga tagagaatcc ggtataaccc
    14461 gccatcttaa ttgctgcgaa aatcgaaccg accagcagcg ccacttccat cagcatattc
    14521 atggcgttac ggatgttatc cgacgcatta cgcaccgacg ggtagcgtcc cagccatttg
    14581 ccgatcgaac gaagcagcaa gacttccgcc gaaataacca ctgcgcctaa taccgctgca
    14641 accatcggat tcggcgagag atagcccacc gcataaacaa aggtaaagcc cgcaactgca
    14701 tacacacccg ttgctaaggc ggtggtggca atcaacggca caaaccccag tccgcgcata
    14761 aattctgcca gagccgcctg attaatcagc gtttgcgatt gttccggcgt cacgcctgcg
    14821 gaatacgctt tctccagtgt gaagatcgaa acttcgctac cagcaaaaat cttcatactg
    14881 gcaacggcgg caatcaatgc tcccacgatg gcgatataag gtaagttttt gataatccgc
    14941 gacgtgcgct cttcaaacac cgaaagcccg ctggcatcga tgtcattttc atcacgatga
    15001 cgcaggtcgt gagttatcgc gatccccagc agcatcacca tgccaataaa gatttcgatg
    15061 gattcagggt taagatgtgg gaaatagcgc acgactacca cacgggtcat cagaacaacc
    15121 acggcggcga ccagactttg cttccagcca aactggtaga aaatcgccac cagcgggaac
    15181 agtgcaaaag cagaaaccac cggcgagctt aattccccga ggctacctaa tacatccacc
    15241 ggcagcgcgg tcagcagctg gttaactggc aacaggcaag taaggatcaa cacgccccag
    15301 atagcgccaa gaccaaacgc catcaggctg tttatcgcca gtacgccgag gatatcggta
    15361 ggaagaaaga gtaaccatgc gttgagcagc ccggttttca gggtaaaaga gatccccacc
    15421 gaggccacaa aaccaatgct cagaccaaaa gcgatactcc ccgcctcgcg acggttcata
    15481 tag
//
LOCUS       DADLTY010000098        15343 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.098, whole genome shotgun sequence.
ACCESSION   DADLTY010000098 DADLTY010000000
VERSION     DADLTY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.098"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="JN334_002615"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="JN334_002615"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAY7438714.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="JN334_002616"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="JN334_002616"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAY7438715.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="JN334_002617"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="JN334_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HAY7438716.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="JN334_002618"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="JN334_002618"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HAY7438717.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="JN334_002619"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="JN334_002619"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HAY7438718.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="JN334_002620"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="JN334_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HAY7438719.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="JN334_002621"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="JN334_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HAY7438720.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="JN334_002622"
     CDS             complement(8064..9206)
                     /locus_tag="JN334_002622"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HAY7438721.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..10228)
                     /locus_tag="JN334_002623"
     CDS             complement(9215..10228)
                     /locus_tag="JN334_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HAY7438722.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="JN334_002624"
     CDS             complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="JN334_002624"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HAY7438723.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="JN334_002625"
                     /gene_synonym="rfaI"
     CDS             complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="JN334_002625"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HAY7438724.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="JN334_002626"
     CDS             complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="JN334_002626"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HAY7438725.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12826..13950)
                     /locus_tag="JN334_002627"
     CDS             complement(12826..13950)
                     /locus_tag="JN334_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAY7438726.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="JN334_002628"
     CDS             complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="JN334_002628"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HAY7438727.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4381 a         3445 c         3144 g         4373 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataaaaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat
     9481 ttttttcttc aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt
     9541 tagcatgatg tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta
     9601 taattgactc atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt
     9661 cccgtaaatt aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac
     9721 acaaacgctc agcgctttta ctttgcatag catctacatc aattaccact gccccatatt
     9781 catctttaaa ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat
     9841 caagatataa taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat
     9901 aaatagatac tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat
     9961 gatataattt aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact
    10021 ctttgtctaa gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa
    10081 gaattgaagc aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga
    10141 cattaaacgt ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt
    10201 taaattgagt aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc
    10261 tcttcaatcc tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata
    10321 taagatatgt atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat
    10381 caatgcgatc ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc
    10441 ttaaaccttt ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat
    10501 gctgatgcaa ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca
    10561 aatactcatt taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga
    10621 aatttaatgt tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac
    10681 cacgtacccg atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt
    10741 ttaagaaacg ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac
    10801 gagcagtgtc aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt
    10861 taaccctgca agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta
    10921 tgaaaacttt caacccattt atcgttttat tatatatcat aatgaattat tttaacctta
    10981 aatctttaga agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa
    11041 tgcttttgtt taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta
    11101 agtaaatctt ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc
    11161 ggataatcag cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg
    11221 tcatcattga ctggattatc aactttgtct tttaattcat agttaatact atatcgggta
    11281 ttatatttct ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga
    11341 tcaaggtggg tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac
    11401 gaaatgttat ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat
    11461 ccagaagcta actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct
    11521 ctttcagcta caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta
    11581 atactaccct tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag
    11641 aaataatctg caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt
    11701 aatgacttca gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt
    11761 gctaaatcag aaaaatataa tttgtcttta tcactgatat aatctgtgaa aacatgaaat
    11821 tcacaagaga tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa
    11881 agaaaatttc tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga
    11941 taatgaaaat tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt
    12001 ctgtatccaa aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt
    12061 tccctgattt ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga
    12121 atgtctttaa gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc
    12181 aggccaatat tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct
    12241 ggaacgcgcg tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct
    12301 tccttaccgg aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta
    12361 atgcccgcag catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt
    12421 acgcgaacat ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt
    12481 ggtgtcagat cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg
    12541 ccgaatgcca ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc
    12601 cattcgcggt ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct
    12661 ttcagggtcg tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc
    12721 agagtacggc gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca
    12781 aaaggatctt tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc
    12841 acccgttatg atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata
    12901 atgtcgcgca ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa
    12961 aatctcattc aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc
    13021 gacaatataa tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat
    13081 cgcttccagc agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc
    13141 cgccgccatt aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac
    13201 gccgcgtttt tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag
    13261 caatgtgttg tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc
    13321 acctttacgc gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc
    13381 attcttctta cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg
    13441 ataaatcccc ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa
    13501 atcggcgatt tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt
    13561 tgcccgctca aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt
    13621 ttcctgcgct actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg
    13681 cattttgttg aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca
    13741 ggcaaaatac tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcac
    13801 ttcaaataca tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc
    13861 tcgggcggcg actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa
    13921 atatttatat aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt
    13981 aatttatcga cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt
    14041 cggtcacgct gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg
    14101 ttatttgacc agggacgcca gaaaatatga tctgttgcgc caaacagcga tatcagcggc
    14161 gtattaactg cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca
    14221 tgatcgatta acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt
    14281 ggcgtctggc atccctgcgc aatttcattg acgcaggcca gatcgtcttt atctgggccg
    14341 gacgtcagaa cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg
    14401 gcgttgtccc agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt
    14461 ccaacaccag cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc
    14521 atggttgtct gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt
    14581 tccaccacat ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca
    14641 gactgccgat gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt
    14701 atagcaacca tccattgatc ggtaagattg acgataaggt catacttatt ggcacgtaat
    14761 accttgatga gatgaaaaaa gttggcaatt ttttctgagg cttttgcttt tttatttttt
    14821 atgccgtaga gcgcgttaat ctctggattt tcagacagga tcgggatggt gtcctgataa
    14881 agcagcacat cgatttttgc gtcagggtaa ttttttttca gagaactaat gacgggagta
    14941 gttaataaca tatccccatg aaaacgcatc ttaatgagca aaattcttcg aaatggctta
    15001 tccacaagta actctttttg ttgagattat ccgattaagg tgaagcagaa aaagcacgct
    15061 accgccccag gctcaacagc tacctgaata ctgataccgc actgatattg acgcgctagt
    15121 gtatcacttc tttcgcggca atccgacccg aatgattttt aagcacattg ttgattttca
    15181 ataaagaaat caatcaaata aaatcattag caaccatatt ctttcactgt tgctctgcga
    15241 ttccttcctg cacgtacagc agaaatcaat gaatcgcata aaaatacaaa aaaagtatag
    15301 atgttcaaca agaattggcg ggtaaattgc tatttaagtc aaa
//
LOCUS       DADLTY010000099        15269 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.099, whole genome shotgun sequence.
ACCESSION   DADLTY010000099 DADLTY010000000
VERSION     DADLTY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15269
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.099"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..73)
                     /locus_tag="JN334_002629"
     CDS             complement(<1..73)
                     /locus_tag="JN334_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HAY7438728.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
     gene            complement(171..1280)
                     /gene="anmK"
                     /locus_tag="JN334_002630"
     CDS             complement(171..1280)
                     /gene="anmK"
                     /locus_tag="JN334_002630"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAY7438729.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1554..2021
                     /gene="slyB"
                     /locus_tag="JN334_002631"
     CDS             1554..2021
                     /gene="slyB"
                     /locus_tag="JN334_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HAY7438730.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="JN334_002632"
     CDS             complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="JN334_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HAY7438731.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2703..2939
                     /locus_tag="JN334_002633"
     CDS             2703..2939
                     /locus_tag="JN334_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAY7438732.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2942..3799
                     /locus_tag="JN334_002634"
     CDS             2942..3799
                     /locus_tag="JN334_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7438733.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3799..5811
                     /locus_tag="JN334_002635"
                     /pseudo
     CDS             3799..5811
                     /locus_tag="JN334_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="JN334_002636"
     CDS             complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="JN334_002636"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HAY7438734.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6414..7310)
                     /locus_tag="JN334_002637"
     CDS             complement(6414..7310)
                     /locus_tag="JN334_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAY7438735.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7359..7598)
                     /locus_tag="JN334_002638"
     CDS             complement(7359..7598)
                     /locus_tag="JN334_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HAY7438736.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7701..8300
                     /locus_tag="JN334_002639"
     CDS             7701..8300
                     /locus_tag="JN334_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7438737.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8337..9434
                     /gene="nemA"
                     /locus_tag="JN334_002640"
     CDS             8337..9434
                     /gene="nemA"
                     /locus_tag="JN334_002640"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HAY7438738.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9515..9922
                     /gene="gloA"
                     /locus_tag="JN334_002641"
     CDS             9515..9922
                     /gene="gloA"
                     /locus_tag="JN334_002641"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAY7438739.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10025..10672
                     /gene="rnt"
                     /locus_tag="JN334_002642"
     CDS             10025..10672
                     /gene="rnt"
                     /locus_tag="JN334_002642"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAY7438740.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10765..15223
                     /locus_tag="JN334_002643"
                     /pseudo
     CDS             10765..15223
                     /locus_tag="JN334_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
BASE COUNT         3507 a         4030 c         4016 g         3716 t
ORIGIN      
        1 ccagttgatt aaaggcgcta caaccggaga gaaggaccgg caaaatgata attaacagtt
       61 ttttcatagt catatcccga agactttcct ggtccggagg gcaaataccc tccttaatct
      121 tccaagtgta acggcagaca cgataagaaa aaattcagtt aattctgata tcacgagttg
      181 gcggggaaaa tagcccccag taccgtctcc tggcttgcgc cagtgacgga aggcagattt
      241 cctggtaatc ccgccagcgt ccgccaggca agccaggcga aagccaatgc ttccatgtca
      301 tcgccactaa tgccaacggc atcggtggtg gtgacttctg tgcctagcag taataccgcc
      361 agacgcgcca tgagtagcgg attacgacta cctccaccac ataccatcaa tcgttcgcag
      421 ccaccgctca acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc cagtgtggcc
      481 tgaacatcac ggggatcaac acccggaaaa tggcgtaaat ggcgctccag ccagccgtag
      541 ttaaagtatt cgcgtccggt gcttttcggt gcaggttgcg agaaatacgg gtcgctgagc
      601 atattttgca gcagtgggag aataacttta cccgcccgtg cccactcggc atctttatcg
      661 taaggtttac ctgcctgacg ccagatccag gcatccatca gcatgttacc aggaccggta
      721 tcgtagccgc caaccagctg cccaggaatg agcagtgaca gattggcgat gccgccaata
      781 ttgagcacca ttcgtcgctc ggttgggtga gccagcaagg catgatggaa cgcaggtacc
      841 agcggtgcgc cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc gacaaccgta
      901 attccggtgc gtgccacaat ttgattgtta tcgccaatct gtaaagtgtg tggtgccacg
      961 cccgtcggtt catgccagac ggtttgaccg tgacagccga tcgcaactat atctctcgcc
     1021 tgcaggtttt gttccttaag caaggcattg accgcatcag caaaaagtcg cccgagttga
     1081 gtatcaagct gtccaaactg cgaaagtgta agctgctggc cctggcaaat atccagtaca
     1141 gcctgtttca gagacaccgg gatcggccag ctcaagcttg ccagctgtgc gacccggtgt
     1201 tcatcaattg tcgccaacac aacatcaaca ccatcaaggc tggtgcctga cataacgcca
     1261 ataaagcggc ccgatttcat agttcatcct ttttcaatct gacgtttgcg caccactcaa
     1321 acataaactt ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg tagtaaaaca
     1381 ttatcttcaa atcaataatc atcatgaatg ttttgtttat aattggttga tcctactttc
     1441 attatgattt gctcatattt ggtagaacat gtaaccatgg attcacatat gccatatact
     1501 ttgaccatga gggatgcttg cgtggcgttt catggtgaac aggagatttt tcaatgatta
     1561 aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt aataacgaca
     1621 ccctgtcagg ggatgtttat accgcttctg aagcgaaaca agtacagaat gtcagctatg
     1681 gcaccatcgt taacgtacgt ccggtacaga ttcagggcgg tgatgattcc aacgttatcg
     1741 gtgcaattgg cggtgctgtt cttggtggtt tcttggggaa tactgttggt ggcggaaccg
     1801 ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt cagggcgtac
     1861 agagtgcaat gaacaaaacg cagggtgtcg agctggaaat tcgtaaagac gatggtaata
     1921 ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa cgtgtcgtac
     1981 tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta aataagtttg cgtgtggtca
     2041 ggttactgac cacacgcccc cttcatttca ccctttggcc tgtaactcaa tgatattatg
     2101 ctcaagtttt gcgatgagcg taatcagttg ctccagttcc tctgcggaga tgccatgtaa
     2161 tatttccgcg cgggttttgt taataacagc ttccatttcg ctgatcagtg gctctgcctt
     2221 ttccgtcagt ttaatacgtt tagcccgacg atcgctggca caagtttgac gcgaaattaa
     2281 ccctttttct tccagttggt ccagagtacg gaccagtgat ggctgctcga tgccaatcgc
     2341 ttttgccagt tgaatttgcg actggtctgg aggtaactga tggatattgt gtaacgtaac
     2401 ccaatgggtt tgtgttaact ccagcggttt caggcgatgg tctatcagag cacgccatat
     2461 gcgcaccaac cgtgccagat cagaacctag tggcgattcc aatttcatct ccttataatt
     2521 agcttgctaa gatattatgc ggcttttaga atagtgtgca gcaattgtat tgctaaaaca
     2581 aatgtattgc tgcatttggt taccgtcaga catatttttc agaaattgcg cgtaaatttt
     2641 tcgcacttaa agaatattta ttaatctaac gcaatatatt cggtcgtaaa ggaatctact
     2701 ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca agatctggtg ttcggtgcgt
     2761 ccgtctattt tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc tggcttgtcg
     2821 tacaccgcct gcttcgtggc tggatctacg ccggcgatat ctggcatccc ttgttaatgg
     2881 atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt gcgtggtaac
     2941 tatgtcaatt aaaacaatta agtatttctc aacaatcatt gtagcgatag ttgcggttct
     3001 tgccggatgg tggctgtgga attattacat gcaatctccg tggacacgcg acgggaaaat
     3061 acgcgcagaa caggtttcta tcaccccaca ggtgtcggga cgtattgttg agctgaatat
     3121 aaaagacaac caactggtta acgcggggga acttctgctc acgatcgata aaacgccctt
     3181 tcagatcgcc gagctgaacg cacaggctca gctggcgaaa gcgcaatctg acctcgcaaa
     3241 agccaataac gaagcgaatc gtcgtcgtca tctctcacaa aattttattt ttgccgaaga
     3301 actcgatacc gctaacctca atgttaaagc gatgcaggcc agtgttaatg ccgcacaggc
     3361 gacgctgaaa cagacacaat ggcaactggc gcaaacggaa attcgcgccc cggtgagtgg
     3421 atgggtgact aacctcacta cccgcatcgg cgattacgct gacaccggga aaccgctgtt
     3481 tgcccttgtc gacagccact cgttttacgt cattggttat tttgaagaaa ccaagttgcg
     3541 ccatatccgc gaaggtgcac cagcacaaat taccctctat agtgataaca aaacgttaca
     3601 gggtcacgtt tccagtatcg gtcgggcgat ttatgatcag agcgttgaaa gtgactcaag
     3661 ccttattccg gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac tcgttcccgt
     3721 ccgctttgcg ctggataaag ttccaggcga tgtcacgctg gtgtcgggca ccacctgtag
     3781 cattgccgta ggtcaataat gaacgcatcg tcatggtcct tgcgcaattt gccctggttc
     3841 agggccacgc tggcgcaatg gcgttatgcg ttacgcaata ccattgccat gtgtctggcg
     3901 ctgacggttg cctattattt gaatctggat gaaccctatt gggcgatgac ctcggctgca
     3961 gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg catggctggc
     4021 agtttactgg gagccattgc ggcactgctt cttgccggac atacgctcaa tgagccgtgg
     4081 ttttttctat tgagcatgtc ggcgtggctt ggcttttgta cctaggcctg tgcgcacttc
     4141 acgaataacg tcgcgtatgc atttcaactg gcgggctaca cggctgccat catcgccttt
     4201 ccgatggtta atattactga ggccagccag ctgtgggata tcgctcaggc gcgcgtttgc
     4261 gaggtgatag tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc
     4321 gatgctactg cccttttaac tgcattgaaa aacatgcacg cccgattact ggaacatgcc
     4381 agtttactct ggcagcctga aacaaacgat gccattcgtg cagcacatga gggggtgatt
     4441 gggcagatac tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt
     4501 cgccagcaaa acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc
     4561 atctccagcc tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa
     4621 attctcgaac aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca
     4681 cgtattatcg ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg
     4741 cagcgactac gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg
     4801 caaagcggtg tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc
     4861 gacaacgccg aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc
     4921 gcatggagta ttgcatcgca atgggatgcc ggtgccaatg cattaacgct ggcagcaatt
     4981 agctgcgtac tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc
     5041 acgctggtgt tactttcgct attcagcttt gtggtcaaat ttggcctgat ggtccagatt
     5101 agcgatctgt ggcaattttt actgtttctc tttccactgc tggcgacaat gcagcttctt
     5161 aaattgcaga tgcccaaatt tgccgcactg tgggggcaac tgattgtttt tatgggttcg
     5221 tttatcgctg tcactaatcc cccggtgtat gattttgctg attttcttaa ctataatctg
     5281 gcaaaaatcg ttggcgttgc gctggcttgg ctagcgttcg ccattctgcg tccaggatcg
     5341 gatgctcgta aaagccgccg ccatattcgc gcgctgcgcc gggattttgt cgatcagcta
     5401 agccgccatc caacactgag tgaaagcgaa tttgaatcgc tcacttatca tcacgtcagt
     5461 cagttgagta acagccagga tgcgctggct cgccgttggt tattacgctg gggtgtagtg
     5521 ctgctgaact gttctcatgt tgtctggcaa ttgcgcgact gggaatcgcg ttccgatccg
     5581 ttatcgcgag tacgggataa ctgtatttca ctgttgcggg gagtgatgag tgagcgtggc
     5641 gttcagcaaa aatcactggc ggccacactt gaagaattac agcggatttg cgacagcctt
     5701 gcccgtcatc atcaacctgc cgcccgtgag ctggcggcaa ttgtctggcg gctgtactgc
     5761 tcgctttcgc aacttgagca agcaccaccg caaggtacgc tggcctcgta attacttaat
     5821 tacaccacag gcatagcgtt caccgccacc gcccagcggt ttaggttgat cggacatatt
     5881 atcgccgcca acgtggacca tcagcgcctt gtctttgatt tcatccagtg atttcagacg
     5941 aggcgcgatg acggcatcgg tagctttgcc gtcattattg acgaccagtg caggcagatc
     6001 gcctaaatgc ccggtacctt ctggcccttc atgtttaccg gtattttgtg gatcaagatg
     6061 cccgcctgcg gattccgcgg cgctggcttt gccatctttg gtggctggct ggcagcttcc
     6121 tttggcatga atatggaagc catgttcacc ggggggtaat gctttcagat cgggtgaaaa
     6181 ctccagacct ttatcggttt cagtaatggt gacgctacca attgactgcc caaccccttg
     6241 cgacgtgacg aggttcatct cgactttttc actggcagct tgtgcgccgg tcgcaacaac
     6301 cagcgccaga atggccagac taaaacgttt cataggacct ccgttcattg tgcattcctg
     6361 aataagtgta aaccagtgac acatttttga acggcggcta ttcctaaaag tgcttacggt
     6421 acgtcgtacc ccagtgcagc tttacggata cgaaaccatt gttgacgggt cattttcagt
     6481 gtttctgctt cgacagctgc ccgtacgcgc tcaattttac ctgagccgat aattggcagc
     6541 ggctgcgacg gtaaacgtaa tacccaggcg taaaccacct gttcaatcga gcccgcgttt
     6601 aactcctccg ccaccacagc cagttcatca cgcagcggct ggaaataatc atcattaaac
     6661 agacgaccac caccaaggca ggaccacgcc atcggacgaa cacgcagttg ttgtagttgg
     6721 tcgagcgtgc catccagcag taacggctga tgcaccgggg atatttccac ctgattagtg
     6781 gcaagggtaa acggcagacg tgattgcaac agggcaaatt gcgcaggcgt aaagttcgat
     6841 acgccaaaat gacgcacttt gccgctctga tgcagatgtt tgaacgcgtc cgccacttca
     6901 tcggcatcca ttaacgggtc tggtcggtgg attaacagca gatccagatg atcggtcgcg
     6961 agattaatta gcgactgttc ggcgctctta atgatgtgat tgcggtcagt gatgtaatga
     7021 ccaatgacgt tttcttcacg cgcggtcgtc gcgataccgc atttactgac gatttccatc
     7081 cgttcacgca ggtgaggtgc cagtttcagt gcctcgccaa acgccgcttc gcactgatag
     7141 ccaccataaa tatcagcatg gtccacggtg gtcacgccga gatccagatg ctcttcaata
     7201 aaactgacca gctggcgggc ggacatattc cagtccatca atcgccagta gcccatcaca
     7261 aaacgggaaa actccgggcc ttccggcgca atagtaatac gctgaaccat aatcgcttcc
     7321 tcttatcaga tataagagga gtatacgcaa gattaggttc aaaagagtga tggttgctcc
     7381 ggttcgtctg atgacgctgg cttatttgcg cgtaatttgc gcattaatcg ctgccgacaa
     7441 aggcgcagca cctcttgttt ttcgccatcg ctcattttat tccagttaaa acgctcatcc
     7501 cgactacgaa aacagccgcg acaaaacccg cgttcgtcag actggcaaat tccccggcac
     7561 gggctctgga cgggaaagaa ctctaattgc tccgccacct cgccctcctc agataagatt
     7621 attaccatta ttgaagctgt taatgtccaa agtagcaact ttgcttgcac tagaccaact
     7681 ggtctactac actccaacgc atgaacaaac acaccgaaca tgatactcgc gaacatctct
     7741 tggcgacggg cgagcaactt tgcctgcaac gtggattcac cgggatgggg ctaagcgaat
     7801 tactaaaaac cgctgaagtg ccgaaagggt ccttctatca ctactttcgc tctaaagaag
     7861 cgtttggcgt tgccatgctt gagcgtcatt acgccgcata tcaccagcga ctggctgagt
     7921 tgctgcaatc cggcgaaggt aactaccgcg accgcatact ggcttattac cagcaaacac
     7981 tgaaccagtt ttgccaacat ggaaccatca gtggttgcct gacagtaaaa ctctctgccg
     8041 aagtgtgcga tctgtcagaa gatatgcgca gcgcgatgga taaaggtgct cgcggcgtga
     8101 tcgccctgct ctcgcaggct ctggaaaatg gccgtgagaa ccattgttta accttttgtg
     8161 gcgaaccgct acaacaggca caagtgcttt acgcactatg gcttggcgcg aatctgcagg
     8221 ccaaaatttc gcgcagtttc gagccactgg aaaacgcgct ggcccatgta aaaaacatta
     8281 ttgcgacgcc tgccgtttag caggcatttt ttatcaccag acgaccggga gcctttatgt
     8341 catctgaaaa actgtattcc ccactgaaag tgggcgcgat cacggcggca aaccgtattt
     8401 ttatggcacc gctgacgcgt ctgcgcagta ttgaaccggg tgacattcct accccgttga
     8461 tggcggaata ctatcgccaa cgtgccagtg ccggtttgat tattagtgaa gccacgcaaa
     8521 tttctgccca ggcaaaagga tatgcaggtg cgcctggcat ccatagtccg gagcaaattg
     8581 ccgcatggaa aaaaatcacc gctggcgttc atgctgaaaa tggtcatatg gccttgcagc
     8641 tgtggcacac cggacgcatt tctcacgcca gcctgcaacc tggcggtcag gcaccggtag
     8701 cgccttcagc acttagcgcg ggaacacgta cttctctgcg cgatgaaaat ggtcaggcga
     8761 tccgcgttga aacatccatg ccgcgtgcgc ttgaactgga agagattccg ggtatcgtca
     8821 atgatttccg tcaggccatt gctaacgcgc gtgaagccgc ttttgatctg gtagagctcc
     8881 actctgctca cggttatttg ctgcatcagt tcctttcccc ttcttcaaac catcgtaccg
     8941 atcagtacgg cggcagcgtg gaaaatcgcg cacgtttggt actggaagtg gtcgatgccg
     9001 ggattgaaga atggggtgcc gatcgcattg gcattcgcgt ttcgccaatc ggtactttcc
     9061 agaacacaga taacggcccg aatgaagaag ccgatgcact gtatctgatt gaacaactgg
     9121 gtaaacgcgg cattgcttat ctgcatatgt cagaaccaga ttgggcgggg ggtgaaccgt
     9181 atactgatgc gttccgcgaa aaagtacgcg cccgtttcca cggtccgatt atcggcgcag
     9241 gtgcatacac agtagaaaaa gctgaaacgc tgatcggcaa agggttaatt gatgcggtgg
     9301 catttggtcg tgactggatt gcgaacccgg atctggtcgc ccgcttgcag ctcaaagctg
     9361 agcttaaccc acagcgtgcc gaaagtttct acggtggcgg cgcggaaggc tataccgatt
     9421 acccgacgtt gtaatccaac attgcgagcg gcgtaaagcc gccgctatac taaaacaaca
     9481 ttttgaatct gttagccatt ttgaggataa aaagatgcgt cttcttcata ccatgctgcg
     9541 cgttggcgat ttgcaacgct ccatcgattt ttataccaaa gtgctgggca tgaaactgct
     9601 gcgtaccagc gaaaacccgg aatacaaata ctcactggcg tttgttggct acggcccgga
     9661 aaccgaagaa gcggtgattg aactgaccta caactggggc gtggataaat acgaactcgg
     9721 cactgcttat ggtcacatcg cgcttagcgt agataacgcc gctgaagcgt gcgaaaaaat
     9781 ccgtcaaaac gggggtaacg tgacccgtga agcgggtccg gtaaaaggcg gtactacggt
     9841 tatcgcgttt gtggaagatc cggacggtta caaaattgag ttaatcgaag agaaagacgc
     9901 cggtcgcggt ctaggcaact aatctcctgc cgggcgtgaa ctcatcgcgc ccgcatcttt
     9961 actgcatcga caagtaatat ttgtcataat gcgcgctgca atttatccgt attaagagaa
    10021 tcagatgtcc gataacgctc aacttaccgg tctgtgcgac cgttttcgtg gtttttatcc
    10081 tgttgtgatc gatgttgaaa cagccggatt taacgccaaa accgatgcgc tgcttgagat
    10141 tgccgccatc accctgaaaa tggatgaaca aggctggctg atgccggaca ccacattaca
    10201 tttccacgtc gaaccatttg tcggcgcaaa tttgcaacca gaagccctcg ccttcaacgg
    10261 cattgacccg aacgatcccg atcgcggcgc ggtcagcgaa tacgaggcgc tgcacgaaat
    10321 ttttaaagtt gtacgtaaag gtattaaagc gagcggctgt aaccgcgcca ttatggtggc
    10381 gcacaacgcc aattttgatc acagctttat gatggccgcc gcagaacgcg cctcgctgaa
    10441 acgtaacccg ttccaccctt tcgccacttt tgacactgct gcactggccg ggctggcact
    10501 cggacaaacc gtattgtcaa aggcttgcca gaccgctggc atggacttcg acagcaccca
    10561 ggcgcactcc gcgctgtacg acaccgaacg cactgctgtg ctgttttgtg aaatcgtcaa
    10621 ccgctggaaa cgtctgggag gctggccgct acctgccgcc gaagaggtgt aatcgagtcg
    10681 atgcctgatg acatgcaatg attcaggcat ctatagtgag gctattccac gcatcaagca
    10741 tgatattcac ggggaatagc gttaatggca gataatccag acccttcatc gctcctgccg
    10801 gacgtgtttt caccggcgac ccgcgactgg tttcttcgcg cctttaaaca gccgaccgct
    10861 gtccagtcgc aaacctggca tgtggcggcg cgaggcgaac atgcgctggt gattgcaccg
    10921 actggctccg ggaaaacgct ggcagcattt ctctacgccc tcgatcggct cttccgcgaa
    10981 ggcggcgaag atacccgcga ggcgcataag cgtaaaacct cacgcatcct ctatatttca
    11041 ccgataaaag ccctgggcac cgacgttcag cgcaacttgc agatcccgtt gaagggtatt
    11101 gccgatgaac ggcggcggcg cggcgaaacg gtaattattg atgaagtcca cgcggtagcg
    11161 ggcagtaaac gtggtgcgca tctggcgtta agtctggagc ggctcgatgc gctgctccac
    11221 acctcagcac agcgaattgg cctttctgcc actgtgcgct cagccagcga tgtggcagca
    11281 tttcttggtg gcgatcgccc ggttacggta gtcaacccgc ccacaatgcg ccatccgcag
    11341 atacgaattg tcgtaccggt cgccaatatg gatgatgtct catcggtcgc cagcggcacc
    11401 ggcgaagaca gccatgccgg ccgggaaggc tccatctggc catatattga aacgggtatc
    11461 cttgatgaag tgttgcgcca tcgctcgacc attgtcttta ctaattcacg cgggctggcg
    11521 gaaaaactga cggcacgatt aaatgagctt tacgccgcac gcttacagcg ttccccgtct
    11581 atcgccgttg atgcggccca tttcgagtcg acctccggcg caacctctaa ccgtgtacaa
    11641 agtagcgacg tttttattgc ccgctcacac cacggctccg tctctaaaga acaacgagca
    11701 atcaccgaac aggcgctgaa atcgggtgaa ttacgctgcg tggtcgcaac ctccagtctt
    11761 gaactgggga ttgatatggg cgcggtggat ctggtgattc aggtggcaac gccgctttct
    11821 gttgccagtg ggttacaacg cattggtcgc gccggacatc aggttggcgg tgtatctaaa
    11881 gggctgtttt tcccccgtac ccggcgtgat ttagtcgatt ccgcagtcat tgtagagtgt
    11941 atgttcgcag gcaggctgga aaacctgaca ccaccgcata atcctctcga cgtccttgcg
    12001 cagcaaaccg ttgccgccgc ggcgatggat gcattacagg tagacgaatg gtactcccgc
    12061 gtacgccgta ccgcaccgtg gaaagatctg ccaagacgtg tttttgacgc cacgctggat
    12121 atgctttccg ggcgctatcc ctctggcgat ttttctgctt ttcgccccaa actggtctgg
    12181 aacagggaga ccgggatatt gaccgcccga cctggcgctc aattgttggc ggttaccagc
    12241 ggcggcacca ttccggatcg tggcatgtat agcgtgttat tacccgaagg tgaagaaaag
    12301 gccggttcgc ggcgggtggg tgaactggat gaggagatgg tatatgagtc gcgggtgaac
    12361 gacattatca ctctcggcgc tacctcatgg cggatccagc aaatcacccg cgatcaggtg
    12421 attgtgactc ctgctccggg tcgttctgcc cagctcccct tctggcgtgg tgaaggtaac
    12481 ggacgtccgg ctgaattagg cgagatgatc ggcgattttc ttcatttact ggcggatggc
    12541 gcgttctttt ccgggactat tcccccgtgg ctggcagaag aaaatacgaa cgccaatatt
    12601 caggggttga ttgacgagca acgcaacgcg acgggcatcg ttccggggag tcgccatctg
    12661 gtcctcgaac ggtgccgtga tgaaattggc gactggcgta ttattttgca ctctccctat
    12721 ggaagacggg tgcatgaacc ctgggcgctg gcgattgccg ggcgaataca tgccctatgg
    12781 ggcgctgacg cgtctgtggt cgccagtgat gacagcattg ttgcgcgcat tcctgacact
    12841 gatggtaaat tgcccgacgc cgcgattttt ttgttttaac cagaaaagtt gctgcaaatt
    12901 gtccgcgagg cggtaggcag ctcggcactt ttcgccgccc gttttcgcga atgcgccgcg
    12961 cgggcattat taatgccggg ccgcacgccg ggccatcgca ccccgctttg gcaacaacgg
    13021 ctgcgcgcca gtcagttgct ggaaatcgct cagggatatc cggattttcc ggtcattctc
    13081 gaaaccctac gcgaatgtct gcaagatgtt tatgatcttc ccgcactgga acgtttgatg
    13141 cgtcgcctga acggtggcga aattcaaata tccgatgtaa cgaccactac gccctcgcct
    13201 ttcgccacaa gtttattgtt cggctatgtc gcggaattta tgtaccagag cgacgccccg
    13261 ctggcagagc gccgggcatc cgtactgtcg ctggacagcg agttactgcg caatctactc
    13321 ggacaggtcg atccggggga attactcgac ccgcaggtca ttcgccaggt ggaagaagag
    13381 ttgcaacgac tggctcctgg tagaagagcg aaaggtgaag aaggattgtt cgacctgctg
    13441 cgcgaactgg ggccaatgac cgttgaagac ctggcgcaac ggcatacagg cagcagtgaa
    13501 gaggttgcgt cgtatctgga aaatcttctt gcagtaaaac gaatcttccc agcgatgatt
    13561 agcggacagg agcgtcttgc ctgtatggat gatgccgcca ggctgcgtga tgccctcggc
    13621 gtacggctac cagagtcatt gccagagatt tatttacata gagtcagtta cccgcttcgc
    13681 gacctctttc tgcgctatct ccgggctcat gctctggtca cggctgaaca actggctcat
    13741 gagtttagtc tcggtattgc cattgtcgaa gagcagcttc agcaactgcg tgaacagggt
    13801 ctggtgatga atctgcaaca agacatctgg gtgagcgatg aagtatttcg tcgtctgcgt
    13861 ttgcgctcgc tgcaagccgc cagagaagcg acgcgtcccg ttgcagccac gacctatgcg
    13921 cgattgctgc tggaacgtca gggcgtatta cccgccaccg atggtagccc ggcgctcttt
    13981 gcctcaacat ctccaggcgt ttatgagggc gtagatggag tgatgcgggt gatcgaacag
    14041 cttgccggag tcggtttacc cgcctcactc tgggaaagcc agatcctgcc tgcccgcgta
    14101 cgcgactatt cgccagaaat gctcgatgaa ttactggcaa ccggagcggt tatctggtcg
    14161 ggacaaaaaa aagctgggtg aagatgacgg cctggtggca ctgcatctac agaaatatgc
    14221 cgcagaatcg ttcactcccg ccgaagcgga tcaggcgaat cgttcggcgc tgcagcaagc
    14281 gataatcgct gttctggctg acggaggagc ctggtttgca caacaaatca gccaacggat
    14341 acgcgataaa atcgtcgaat cggttgattc ctcggcactg caagaggcgt tatgggcgct
    14401 ggtctggcaa ggcgtcatta ccagcgatat ttgggcaccg ttacgcgccc tcacccgcag
    14461 cagttccaac gcacgcacct caactcgccg cagtcaccgg gctcgtcgtg ggcgtcctgt
    14521 ctatgcacaa cccgtctcgc cgcgggtatc ttacaacaca ccaaatctgg ctggacgctg
    14581 gtcgttattg caggtggagc cactaaacga taccgaaagg atgctggcgc tggcggaaaa
    14641 tatgctcgac cgctacggca tcatcagtcg tcaggcggtg atagccgaaa atatccctgg
    14701 cgggtttcca tcgatgcaaa cactttgtcg aagtatggaa gactccgggc gaattatgcg
    14761 aggtcgtttt gtagaaggtc tgggtggcgc gcaattcgct gaacgtctga ctattgaccg
    14821 attgcgcgat ctggcgacac aagccacgca aacacgccac tatacaccag tggcgctctc
    14881 cgccaacgat ccggctaatg tgtggggaaa tcttctgccc tgggctgcac atccggcaac
    14941 gctggtacca acgcgtcggg cgggtgcgct ggtggtcgtt tctggcggca aattgttact
    15001 ctatctggcg caaggcggca aaaaaatgct ggtctggcag gaaaaggagg aattactcgc
    15061 cccagagatt ttccaggcgc tgaccaccgc actgcgtcgc gaaccaaggc tgcgctttac
    15121 gctaacagaa gtgaatgatc ttccggtccg gcaaacgccg atgtttacgc tgctgcgtga
    15181 ggcgggattt tcaagttcgc cacaagggct ggattgggga tagttgacat cctccacgcc
    15241 ctgaaagacg tggttccctg ctacgttca
//
LOCUS       DADLTY010000100        15156 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.100, whole genome shotgun sequence.
ACCESSION   DADLTY010000100 DADLTY010000000
VERSION     DADLTY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.100"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            142..885
                     /locus_tag="JN334_002644"
     CDS             142..885
                     /locus_tag="JN334_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7438741.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(942..2375)
                     /locus_tag="JN334_002645"
     CDS             complement(942..2375)
                     /locus_tag="JN334_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY7438742.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            2420..2730
                     /locus_tag="JN334_002646"
                     /pseudo
     CDS             2420..2730
                     /locus_tag="JN334_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            2894..3553
                     /locus_tag="JN334_002647"
     CDS             2894..3553
                     /locus_tag="JN334_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAY7438743.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(3749..4729)
                     /gene="ygfZ"
                     /locus_tag="JN334_002648"
     CDS             complement(3749..4729)
                     /gene="ygfZ"
                     /locus_tag="JN334_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HAY7438744.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            4972..5238
                     /gene="sdhE"
                     /locus_tag="JN334_002649"
     CDS             4972..5238
                     /gene="sdhE"
                     /locus_tag="JN334_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HAY7438745.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            5219..5626
                     /locus_tag="JN334_002650"
     CDS             5219..5626
                     /locus_tag="JN334_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438746.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(5666..6187)
                     /gene="fldB"
                     /locus_tag="JN334_002651"
     CDS             complement(5666..6187)
                     /gene="fldB"
                     /locus_tag="JN334_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HAY7438747.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            6299..7195
                     /gene="xerD"
                     /locus_tag="JN334_002652"
     CDS             6299..7195
                     /gene="xerD"
                     /locus_tag="JN334_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAY7438748.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            7220..7930
                     /gene="dsbC"
                     /locus_tag="JN334_002653"
     CDS             7220..7930
                     /gene="dsbC"
                     /locus_tag="JN334_002653"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HAY7438749.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            7936..9669
                     /gene="recJ"
                     /locus_tag="JN334_002654"
     CDS             7936..9669
                     /gene="recJ"
                     /locus_tag="JN334_002654"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAY7438750.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALMEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            9760..10858
                     /gene="prfB"
                     /locus_tag="JN334_002655"
     CDS             join(9760..9834,9836..10858)
                     /gene="prfB"
                     /locus_tag="JN334_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAY7438751.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            10868..12385
                     /gene="lysS"
                     /locus_tag="JN334_002656"
     CDS             10868..12385
                     /gene="lysS"
                     /locus_tag="JN334_002656"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAY7438752.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(12428..12976)
                     /gene="idi"
                     /locus_tag="JN334_002657"
     CDS             complement(12428..12976)
                     /gene="idi"
                     /locus_tag="JN334_002657"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAY7438753.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            13031..13102
                     /locus_tag="JN334_002658"
     CDS             13031..13102
                     /locus_tag="JN334_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438754.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(13099..13224)
                     /locus_tag="JN334_002659"
     CDS             complement(13099..13224)
                     /locus_tag="JN334_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438755.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(13226..14674)
                     /locus_tag="JN334_002660"
     CDS             complement(13226..14674)
                     /locus_tag="JN334_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAY7438756.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         3802 a         3752 c         3992 g         3610 t
ORIGIN      
        1 gcactggcgc gaatatttac cctcaccccg accctctccc tgaaagggcg agggggaaaa
       61 gcgtgccaac attgaagatt gagccagttt gttagcaatc tcaaagatac gtcaacgaat
      121 taatttttct cggaaaaaca aatggctata gcacttgtga ccggtggcag tcgcggcatc
      181 gggcgggcaa ctgcattact gttggcgcaa gaagggtata cggtggcggt taattatcag
      241 caaaacctcc acgcggcgca ggaagtgatg aacttaataa tgcaagccgg tggcaaagca
      301 ttcgtgctcc aggcggatat cagcgacgaa aaccaggtcg ttgcgatgtt tacagcaatc
      361 gatcagcacg atgaaccgct agcagcgctg gtcaataacg ccgggatctt gtttacccag
      421 tgcaccgttg aaaaccttac cgcagagcga atcaaccgag tactttccac caacgtgacg
      481 ggatattttc tctgctgccg cgaggcggta aaacgcatgg cgcttaaaaa tggtggcagt
      541 ggcggcgcta tcgtcaatgt ctcttcggtg gcctcacggt tgggttcgcc aggggaatat
      601 gttgattacg cggcatcgaa aggggcgatt gattcgttaa ccaccggact atcgctggaa
      661 gtcgccgcgc aggggatccg cgttaactgc gtgcggccag ggtttattta taccgaaatg
      721 cacgccagcg gcggcgagcc tggacgcgtc gatcgcgtta agtcgaacat ccccatgcag
      781 cgtggtggac aggcagaaga ggtcgcgcag gccattgtct ggctactaag tgataaagcc
      841 tcttacgtca cgggaagttt tatcgatttg gcgggcggga aataaaacag ggaagttgtc
      901 tgaccggatg caacaagtat tgcatccggt acttcatcga cttaaagctt ctcgccgttg
      961 ctggcaatca cctctttgta ccagttaaag ctcttcttac gtgaacgcga catatcgcca
     1021 gtaccgtcgt catgtttatt cacatagata aagccgtagc gtttgctgta ctgcccggta
     1081 gtgaatgaca cgcagtcgat gcagccccac ggtgtgtagc ccatcagatc cacaccatcg
     1141 taagtcactg cttttttcat ctcttcaata tgggcgcgca ggtagtcaat gcggtagtcg
     1201 tcgttgatgc tgccatcttc ttccactttg tcgtaagcgc caaaaccgtt ttcgacaata
     1261 aacagcggcc tctgataacg ctcatacagt tcgcaaaggg catagcgcag acctactgga
     1321 tcaatctgcc agccccagtc agacgcttta acatacgggt tcggtacgct gccttcaaaa
     1381 ccagagatcg catcgccggt gccgccttcg gccttcactg cgttggtcat gtaatagctg
     1441 aaaccaagat aatcgcaggt gccttcgcgc agtacatcga gatcgccgtc ttccattttg
     1501 atattaaatc cgcgacgttc ccactcgttc aatacatagg acgggtaata gccgcatagc
     1561 tgcacatcgg taaagacgta gcgttcgcgc atcgactcct gagcgaacat cacatcgtcc
     1621 gggttacatg agtagggata gagcggcacc atcgccagca tacagccgac tttcatctcc
     1681 gggttaatgc gacgcgcggc tttcactgcc agggcgctgg cgacaaactg gtgatgcagc
     1741 acctggtaca tcgtctcttc cgggttgtca tgctcggtat acaccacgcc ggagcagcag
     1801 taaccaaaca gcggcgcacg ccagttacgc tggttgttta tttcgttgaa ggtcatccag
     1861 tatttgactt tatgcttgta gcgttcgaat accacttccg cgaaacggac aaagaaatca
     1921 acgaatttac ggttggtcca gctaccgtat tgctgcacca gatgcagcgg catttcaaag
     1981 tgggagaggg ttatcaccgg ttcgatgttg tatttcagca gttcatcgaa cagagagtcg
     2041 taaaacttca gcccttcttc gtttggctga gcttcatcac cttttggaaa aatacgcgtc
     2101 caggcgatgg aagtccgaaa acatttgaag cccatttcgg caaatagctt gatgtcttcc
     2161 ttgtagtgac cataaaaatc aacggcttca tggtttggat agtattttcc tggcaagact
     2221 tctttggtga tttcgcgcgg cacgccgtgt gcgccacagg tcagaacgtc acaaatgctc
     2281 ggtcctttgc cgcctttgtt ccagccgcct tcgacctgat gagcggcaac ggcgccaccc
     2341 cataagaaat ctttcggtaa ggtgagtttt ttcactatca taggctccag atcagtcaat
     2401 tcttacagga aatagtacca tgcagccaaa cgacatcact tttttcaacg tttccaggat
     2461 gacattctgg ctgggcgtaa aaccatcacc atccgcgacg agtctgaatc gcacttcaaa
     2521 acgggtgatg tgcttcgtgt cggacgtttt gaagatgacg gttatttttg cacgattgaa
     2581 gtcaccgcaa cctcaaccgt aacgctggat acactgacgg aaaaacatgc agagcaggaa
     2641 aatatgaccc tgcctgaact gaaaaaggtc attgctgaca tctatcccgg tcagacacaa
     2701 ttttatatga ttgaatttaa atgtctttaa attgaactca cgcctgttag ttgaaatttc
     2761 gcatttaatt tcatgatttt taggagatta gtctcaaatc agaatttatt ttagctaaca
     2821 ggtgttcact ggaactattc tcagttacgc tggagaggta tacatgtgcg tgtacgtttc
     2881 tctggagtaa gttatggttc agaagcccct cattaagcag ggatattcgc tggcagagga
     2941 aattgccaac agcgtcagtc acggcattgg gctggtgttt ggtatcgttg ggctggtgtt
     3001 gctactggtt caggcggtgg atctcaatgc cagcgccaca gcgataacca gttacagcct
     3061 ctatggcggc agtatgatcc tgctgttcct cgcttcgacg ctctatcacg ccattcctca
     3121 tcaacgggca aaaatgtggc tgaagaaatt tgaccattgc gctatttacc tgttgattgc
     3181 cggaacctac acgccgtttt tgctggtggg gctggattct ccgttagcgc gcgggttgat
     3241 gattgttatc tggagcctag cattgctggg gattctgttc aaactgacca tcgcgcaccg
     3301 attcaaaatt ttatctctgg tgacttatct ggcgatgggc tggctgtcgc tggtggtaat
     3361 ttatgaaatg gcagttaagc tcgcggcggg cagcgttacc ttactggcgg taggcggcgt
     3421 ggtttattcg ctcggggtga ttttctacgt ctgcaaacgc attccataca accatgccat
     3481 ctggcacggc ttcgtgctcg gcggtagtgt gtgccacttt ctggcgatct atttgtatat
     3541 tgggcaggcg taatgggtat gcctttgtag gcctgataag acgcgtcagc gtcgcatcag
     3601 gcatcggagc gcaaccgccg gatgcggcgt gaacgcctta tccgatctac gggcctcgtc
     3661 cctgttgtga taaaggcatc aaatcccaga catgataaga cgcgtcagcg tcgcatcagg
     3721 cgataaatgc ctgatgcgat acagagattt actcttcgag cgaatacggc agcggctcga
     3781 tatgcagtgt attcgcatcg tcgcgtacgc ggaagatgct gtccggttcc atatcgttat
     3841 tcatgacgac ctgtaccacg acctgaccat cttccagttt taccgcagcc agcaccgtac
     3901 cggtacgacg ccagttctcg cccattttca gctctaagtc ttcaccagct tccggcagtc
     3961 ggctggcact acctgccagc aaccagagcg cgcgtttgtt ggcaccacgg aattttgctc
     4021 gcgccaccat ctcttgtccg gtataacagc ctttcttaaa gctgataccg cccagcgcct
     4081 ggagattggt cgcctgtggg ataaactgcc cgctgttggc ggcatcaatc accgggaaac
     4141 cagcttcaat gtttaatgcc agccactgtt ggctattgtt cagttccgct tcaccgcgca
     4201 gtttatcggt cagcatatta gcagtagctt catcggttac gatcaggaaa cgttctgccg
     4261 ggtgttcaaa ccatagcaaa gtggtcgcgc cttctttgac tacctgtttt tctttcgaag
     4321 gcagttcgct aaagagattt gccagcgcgg cgcgcgcctg aaaaccggca acaccaagca
     4381 gcacacgctc gtcgtctggc gcgatggtca ctttagagaa taccgcatat tttttcagtt
     4441 cagtcagctg cggttcacgc acgctgcgcc gttcaatcca tgcaaagcca tcgccgtcgc
     4501 ggaacagacg taaattgctc cacattttac ctttggcgtc gcaatgggcg gcgagcaggt
     4561 gctgatcttc tgccatctgg ctgacatctg ctgtcacctg accctgcata tatttttcgc
     4621 tgtccgcgcc agtaatggtg gcaagcgccc agtcatcaag cgtcatcagc gtcagcggta
     4681 aacgggcaga agccgtaggc tgacgtggag gaaaaggtgt aaaagccata atagagtcct
     4741 gagttgctta acggcgtatt aatgactaat ggtaaaagag ctatgaggca atgcaagtgc
     4801 tttcactacg cgatctgtgc cttctgcgag gcgactgcca aaaaaatgaa gcccttgaat
     4861 tgttgtgaat taatttcgcg atcgcgatgg tgtttactga tattcccgta aaagaagtcg
     4921 aaaaacacgt tacaatagcc gggtactgga ttttcgtgag acaggaagaa gatggacatt
     4981 aacaacaaag cccgaattca ttgggcatgc cgccgtggta tgcgcgaact cgatatttca
     5041 atcatgccgt ttttcgaaca tgagtacgac agcttaagcg atgacgaaaa acgcatcttt
     5101 attcgtctgc tggaatgtga cgatccggac ctgtttaact ggctgatgaa tcacggtaaa
     5161 ccggccgatg cagaactgga aataatggtc cgactcatcc agacacggaa ccgggaacgt
     5221 ggtcctgtgg caatctgatt tgcgcgtctc ctggcgcgca cagtggcttt ccttgctgat
     5281 tcatgggctg gttgccgctg ttattttact catgccctgg ccgctcagtt acaccccgtt
     5341 atggatggtg ttactttcgc tggtggtatt tgattgcgtt cgcagccagc ggtgtattaa
     5401 tgctcgccag ggggaaattc gcttgttgat ggacgggcgt ttgcgttggc aagggcagga
     5461 gtggagcatc gtcaaagcac cgtggatgat taagagcggc atgatgctgc gtttacgttc
     5521 tgatggcggt aaacggcaac atttatggct ggcagccgac agcatggacg aagccgaatg
     5581 gcgggattta cggcggattt tgttgcaaca agagacgcaa agataaagat gacggcaaag
     5641 catatgcgct aagccgtcag tgagatcagg cgtaatgctc tgccatttcg ttgaggattt
     5701 gctcgcacca gctctgaata cgctcgtcgc taaggtcata ctggttagtt tcatccagcg
     5761 ccagacccac gaacagttgc ccgtcagcaa tcaccggttt cgggctggta aattcatatc
     5821 cttccgttgg ccagtagccg acgaacttca cgcctttggt cgagagtttg tcatgcagca
     5881 taccgagcgc atcgaggaac cactcgccgt atcccagttg atcgccaagc ccatacagcg
     5941 caataatttt accttcaagg ttcaggtcgt cgagctgatc ccagacggct tcccagtctt
     6001 cctggatttc accaaaatcc caggtcggga tacccagaat gagcacatcg tactgctcca
     6061 ttaatttcgg ggagtcgtcc ttgaggttat gtaaggtcac cagttctggg ccgataatat
     6121 cgcggatttt ttctgccgcc atttcggtgt aacaggtgct ggaaccgtaa aaaagaccca
     6181 tattcataac gtaagcttct cgattctgct gtggcttttg cagctagtgt atcagaatcg
     6241 ctttagtcac ggcacaataa ggcataatgg atcaaatgag tgaccataag gggcgcaagt
     6301 gaaacaggag ctggcacgca tcgagcagtt tcttgatgct ctgtggctgg aaaaaaatct
     6361 ggctgaaaat acgttgaacg cttaccgtcg cgatctgtca atgatggtgg agtggttgca
     6421 tcaccgcggg ttgacgctgg cgacggcgca aagtgacgat ttgcaggcat tactggcaga
     6481 acggctggag ggcgggtata aagccaccag ctcagcgcgt ttgctgagtg cagtgcgccg
     6541 attgttccag tatctttatc gcgaaaagtt tcgtgaagac gatcccagtg cgcatctcgc
     6601 ttcaccgaaa ttgccccagc gtttgccaaa agatttaagc gaagcgcagg tcgaacgttt
     6661 attacaggcg ccattaattg atcagccact ggagctacgc gataaagcca tgcttgaagt
     6721 gttgtatgct accgggctgc gtgtctctga actggtcggg ctgacaatga gtgatatcag
     6781 cctgcgtcag ggcgtagtgc gggtcattgg taaaggcaac aaagagcgcc tggtgccgtt
     6841 aggggaagag gcggtttact ggctggaaac ctatctggaa catgggcgtc cgtggctgct
     6901 gaatggtgtg tcaattgacg tgttgtttcc cagccagcgt gcgcagcaga tgacgcgaca
     6961 gaccttctgg caccgcatta aacattatgc cgtgctggcg ggtatcgaca gcgaaaagct
     7021 gtcaccgcat gtgttgcgtc acgcttttgc cactcattta ttaaatcatg gtgcggattt
     7081 acgcgtggtg cagatgctac tgggccacag cgatctttcc accacgcaaa tttatacgca
     7141 tgtcgctacc gagcgtctgc ggcaacttca tcaacagcat cacccgcggg cgtgatgtct
     7201 gaaaagaacg ggaagattta tgaagaaagg ttttatgttg tttactttgt tagcggcgtt
     7261 ttcaggcttt gctcaggctg atgacgcggc aattcaacaa acgttagcca aaatgggcat
     7321 caaaagcagc gatattcagc ccgcgcccgt agctggcatg aagacagttc tgactaacag
     7381 cggtgtgttg tacatcaccg atgatggtaa acatatcatt caggggccaa tgtatgacgt
     7441 tagtggcacg gctccggtca atgtcaccaa taagatgctg ttaaagcagt tgaatgcgct
     7501 tgaaaaagag atgatcgttt ataaagcgcc gcaggaaaaa cacgtcatca ccgtgtttac
     7561 tgatattacc tgtggttact gccacaaact gcatgagcaa atggcagact ataacgcgct
     7621 ggggatcacc gtgcgttatc ttgctttccc gcgccagggg ctggacagcg atgcagagaa
     7681 agaaatgaaa gctatctggt gtgcgaaaga taaaaacaaa gcgtttgatg atgtgatggc
     7741 aggtaaaagc gtcgcaccag ccagctgcga cgtggatatt gccgaccatt acgcacttgg
     7801 cgtccagctt ggcgttagcg gtactccggc agttgtgctg agcaatggca cacttgttcc
     7861 gggttaccag ccgccgaaag acatgaaaga attcctcgac gaacaccaaa aaatgaccag
     7921 cggtaaataa ttcgcgtgaa acaacagata caacttcgtc gccgtgaagt cgatgaaacg
     7981 gcagacttgc ccgctgaatt gcctcccttg ctgcgccgtt tatacgccag ccggggagta
     8041 cgcagtgcgc aagaactgga acgcagtgtt aaaggtatgt tgccctggca gcaactaagc
     8101 ggtgtcgaaa aggccgttga gatcctttac aacgcttttc gcgaaggaac gcggattatt
     8161 gtggtcggtg atttcgacgc cgacggcgcg accagcacgg ctctaagcgt gctggcgatg
     8221 cgctcgcttg gttgcagcaa tatcgactac ctggtaccaa accgtttcga agacggttac
     8281 ggcttaagcc cggaagtggt cgatcaggcc catgcccgtg gcgcgcagtt aattgtcacg
     8341 gtggataacg gtatttcctc ccatgcggga gttgagcacg ctcgctcgtt gggcatcccg
     8401 gttattgtta ccgatcacca tttgccgggc gacacattac ccgcagcgga agcgatcatt
     8461 aaccctaact tgcgcgactg taatttcccg tcgaaatcac tggcaggcgt gggtgtggcg
     8521 ttttatctga tgctggcgct gcgcaccttt ttgcgcgatc agggctggtt tgatgagcgc
     8581 ggcatcgcaa ttcctaacct ggcagaactg ctggatctgg tagcactggg gacagtagcg
     8641 gacgtcgtgc cgctggacgc taataatcgc attctgacct ggcaggggat gagtcgcatc
     8701 cgtgccggaa agtgccgtcc agggattaaa gcgctgatgg aagtggcaaa tcgcgatgca
     8761 caaaaactag ccgccagcga tttaggtttt gcgctgggac cgcgtctcaa tgctgccggg
     8821 cgactggatg atatgtccgt tggtgtggcg ctgttgctgt gcgacaacat cggcgaagcg
     8881 cgcgtgctgg caaatgaact cgatgcgcta aaccagacgc gaaaagagat cgaacaagga
     8941 atgcaggttg aagccctgac cctgtgcgag aaactggagc gcagccgtga cacgctaccc
     9001 ggcgggctgg caatgtatca ccccgaatgg catcagggcg ttgtcggtat tctggcttcg
     9061 cgtatcaaag agcgttttca ccgtccggtt atcgcctttg cgccagcagg tgacggtacg
     9121 ctgaaaggtt ccggtcgctc cattcagggg ctgcatatgc gtgatgcgct ggagcgatta
     9181 gacacactct accccggcat gatgctcaag tttggcggtc atgcgatggc ggcgggtttg
     9241 tcgctggaag aggataaatt cgaactcttt caacaacggt ttggcgaact ggttactgag
     9301 tggctggccc cttcgctatt gcaaggcgaa gtggtgtcag acggtccgtt aagcccggcc
     9361 gaaatgacca tggaagtggc gcagctgctg cgcgatgctg gcccgtgggg gcagatgttc
     9421 ccggagccgc tgtttgacgg tcatttccgt ctgctgcaac agcggctggt gggcgaacgt
     9481 catttgaagg tgatggtcga accggtcggc ggcggtccac tgctggatgg tattgctttt
     9541 aatgtcgata ccgctctctg gccggataac ggcgtgcgcg aagtgcaact ggcttataag
     9601 ctcgatatca acgagtttcg cggcaaccgc agcctgcaaa ttatcatcga caatatctgg
     9661 ccaatttagc gtcatcttct ctataaaaaa gagcgtggat tgggtacaat cccgctctta
     9721 tcaccgcatt ttgactagct caataaaaga aatcagacca tgtttgaaat taatccggta
     9781 aataatcgca ttcaggacct cacggaacgc tccgacgttc ttagggggta tctttgacta
     9841 cgacgccaag aaagagcgtc tggaagaagt aaacgccgag ctggaacagc cggatgtctg
     9901 gaacgaaccc gaacgcgcac aggcgctggg taaagagcgt tcctccctcg aagccgttgt
     9961 cgacaccctc gaccaaatga aacaggggct ggaagatgtt tctggtctgc tggaactggc
    10021 tgtagaagct gacgacgaag aaacctttaa cgaagccgtt gctgaactcg acgccctgga
    10081 agaaaaactg gcgcagcttg agttccgccg tatgttctct ggcgaatatg acagcgccga
    10141 ctgttacctc gatattcagg cggggtctgg cggtacggaa gcacaggact gggcgagcat
    10201 gcttgagcgt atgtatctgc gctgggcaga atcgcgtggt ttcaaaactg aaatcatcga
    10261 agagtcggaa ggtgaagtgg cgggtattaa atccgtgacg atcaaaatct ccggcgatta
    10321 cgcttacggc tggctgcgta cagaaactgg cgttcaccgc ctggtgcgta aaagcccgtt
    10381 tgactccggc ggtcgtcgcc acacgtcgtt cagctctgcg tttgtttatc cggaagttga
    10441 tgatgatatt gatatcgaaa tcaacccggc ggatctgcgc attgacgttt atcgcgcgtc
    10501 cggcgcgggc ggtcagcacg ttaaccgtac cgaatctgcg gtgcgtatta cccacatccc
    10561 gaccgggatc gtgacccagt gccagaacga ccgttcccag cacaagaaca aagatcaggc
    10621 catgaagcag atgaaagcga agctttatga actggagatg cagaagaaaa atgccgagaa
    10681 acaggcgatg gaagataaca aatctgacat cggctggggc agccagattc gttcttatgt
    10741 ccttgatgac tcccgcatta aagatctgcg caccggggta gaaacccgca acacgcaggc
    10801 cgtgctggac ggcagcctgg atcaatttat cgaagcaagt ttgaaagcag ggttatgagg
    10861 aaccaacatg tctgaacaac acgcacaggg cgctgacgcg gtagtcgatc ttaacaatga
    10921 actgaaaacg cgtcgtgaga agctggcgaa cctgcgtgag cagggaattg ccttcccgaa
    10981 cgatttccgt cgcgatcata cctctgacca attgcacgca gaattcgacg gtaaagagaa
    11041 cgaagaactg gaagcgctga acatcgaagt cgccgttgct ggccgcatga tgacccgccg
    11101 tattatgggt aaagcgtctt tcgttaccct gcaggatgtg ggtggccgca ttcagctgta
    11161 cgttgcccgt gacgatctgc cggaaggcgt ttacaacgag cagttcaaaa aatgggacct
    11221 cggcgacatc ctcggcgcga aaggtaaact gttcaaaacc aaaaccggcg aactgtctat
    11281 ccactgtacc gagctgcgtc tgctgaccaa agcactgcgt ccgctgccgg ataaattcca
    11341 cggcttgcag gatcaggaag cgcgctatcg tcagcgttat ctggatctca tctctaacga
    11401 tgaatcccgt aacaccttta aagtacgctc gcagatcctc tctggtattc gccagttcat
    11461 ggtgaaccgc ggctttatgg aagttgaaac gccgatgatg caggtgatcc caggcggtgc
    11521 cgctgcgcgt ccgtttatca cccaccataa cgcgctggat ctcgacatgt acctgcgtat
    11581 cgcgccggaa ctgtacctca agcgtctggt ggtcggtggc ttcgagcgtg tattcgaaat
    11641 caaccgtaac ttccgtaacg aaggtatttc cgtacgtcat aacccagagt tcaccatgat
    11701 ggaactctac atggcttacg cagattacaa agatctgatc gagctgaccg aatcgctgtt
    11761 ccgtactctg gcacaggata ttctcggtaa gacggaagtg acctacggcg acgtgacgct
    11821 ggacttcggt aaaccgttcg aaaaactgac catgcgtgaa gcgatcaaga aatatcgccc
    11881 ggaaaccgac atggcggatc tggataactt cgactctgcg aaagcgattg ctgaatctat
    11941 cggtatccac gttgagaaga gctggggtct gggccgtatc gttaccgaga tcttcgaaga
    12001 agtggcagaa gcacatctga ttcagccgac cttcattact gaatatccgg cagaagtttc
    12061 tccgctggcg cgtcgtaacg acattaaccc ggaaatcaca gaccgctttg agttcttcat
    12121 cggtggtcgt gaaatcggta acggctttag cgagctgaat gacgcggaag atcaggcgca
    12181 acgcttcctg gatcaggttg ccgcgaaaga cgcaggtgac gacgaagcga tgttctacga
    12241 tgaagattac gttaccgcac tggaacatgg cttaccgccg acagcaggtc tggggattgg
    12301 catcgaccgt atggtaatgc tgttcaccaa cagccatacc atccgcgacg ttattctgtt
    12361 cccggcgatg cgtccggtga aataagcatt acgttatgct cacaaccccg gcaaatgtcg
    12421 gggtttttta tttaagctgg gtaaatgcag ataatcgtat tctggcttcg cgatttgtcg
    12481 cctgcatcac catccacgga ctgaacgccc acggcgtggc atcaataccg cgtaatacat
    12541 cagctaaatc acaccattga taatccatca cttcatcatc gttgatctgt aacgcactgg
    12601 tggtgcgtgc ggcaaatacc ggacacactt cattttccac aatgcctctc ggatcggtgg
    12661 cgcggtagcg aaagtcagga tagatagatt caggaggcgt aatttccacg ccaagctcat
    12721 aacggcaacg gcggatcacc gcgtcttcgc tgctttctcc cagttgtggg tgcccacaaa
    12781 ccgagttagt ccacacgcca ggccacgctt ttttgctcag tgcgcggcgg gtaactaata
    12841 attgcccctt ggcattaaac agccaactgg agaacgcgag atgtaagagg gtgtctgccg
    12901 tgtgtgcggc atacttttcc agcgtacccg tgggaactcc ctgtgcattc aataaaatga
    12961 cgtgttccgt ttgcataatt tctcacatgt aattctgatc actcatcatt atagataatt
    13021 gaagagtgaa atgattaacc aagagagcgt ttatcgacaa ccgcccgttt tgagcggatg
    13081 ccgacaggta aaaaccattt aaaaaagtag catcatatat ttgaagcgac tttcaatggc
    13141 cattgcaaca cagtcagaaa tgacaggaat agagagagta aaaagcaaca acagactgaa
    13201 tatagcgcgc attaaaaaat tcatattatt ctccatgctc atttttcttc agcttgcgca
    13261 tcagaagcca catacgtact gtcctgactt ctaaatcttt atcagagacg gattccttca
    13321 ccaggtcagc atgatgctga tagccattaa agaagacgtt cagcacaacg gcgctaaggg
    13381 ttgcgagcat aatgccgcta tgtagcagcg gttgcagtac ggccggtaac ttagaaaaga
    13441 aatcgtgaga gagcgtcgga gtcatgccaa cgccgagact gatcgccaca atatagaggt
    13501 tgtaacggtt ggtggtgtag ttacagcgcg acagaattcg aatccctgtc gccagtacca
    13561 tgccgaacat cactagacca gcgccgccca gcacaaattg cggaatggag gctaccagca
    13621 ccgccatttt tggcaccatg ccaaacagga ttaatataat tcccgaagaa atacacaccc
    13681 agcggctatg aacgcgcatc acgctaacca ggccaacgtt ttgagaaaat gacgtgtggg
    13741 ggaagctgtt aaacgtgccg cctatcattg tccctacgcc atcgacacgc agcccgcaaa
    13801 taatatcgtg tgaagagagt ttacgaccga ctatttcacc cagtgccagg aacatcccca
    13861 tcgactcgat aaacacgatg attaacacgg cagtcatggt cagaatggaa acgggatcga
    13921 aaatcggcat accaaacgac atcggggtga caatcgcaaa ccacgaggca tcatgtagcc
    13981 cggataaatt gacttcattc atcatccacg aaagtaaaaa gccaaataca atccccagta
    14041 atacggcgac gttggacata aatcctttcg catagcgagt aatgagcaag ataaaaatta
    14101 agacggcaaa ggagatacct aaataaacgg gattaccata ttgcggattc cctttacctc
    14161 cagcggccca gtcaataccc acctgaatga tgctaagccc aatagaagta ataaccacac
    14221 cggtaaccag tggcgggaat aaaggcatca agcgaccaat aagtggcgct aataatgtgg
    14281 tgataaaacc cgcggcgata gtggcaccaa atatccccag caggccgata tccgggttca
    14341 tcccaatggc tatcattggt gttacagcag caaaggtcac cgacataatc accggcaggc
    14401 ggatccccat aaagcggccg ataccgatac attgcaataa tgtgacgatc ccgcagcaaa
    14461 agagatccga gctaatgagc atcgcaatag catcttttga gaggcccagt cggtcaccaa
    14521 tcataagagg aacagcgact gcacctgcgt acatgacaag gacgtgttgc agaccgagta
    14581 tgatcagctt tcctggtgat aatatgcggt caacctcatc agttgggcgg tcttgcccag
    14641 aagatgaggg aagttgggaa tctatggcgc tcatcagtgt ttctcctttc gcccgccaga
    14701 ataaaaagca accgtttacg ttaatgaaac tgtctaaaca catgctcgtg gagtgccagt
    14761 ttttgcgagg aaggggagtt agagacagga attgcggagg caatcacata atcgtgctta
    14821 aaccacgaaa atgacaagag aaactgacaa tggaattatc tgtaattttc tcatattgat
    14881 atttcattaa tcattctggc atgtcgcact ctcgcattta atcgttttta tctggatagc
    14941 gctcttttga tcggcaatgt tgaaataatg aaagcggcat agattgaggg gcaataattt
    15001 gaactcagtc gcaaaattat aattaaaacg gtcatgctac ctaatatatg cccgtttatt
    15061 gcatcgcgaa atttgagaag acataactca ttaaatgaaa gggatgcaaa tgaatacgtt
    15121 tatcgctgct gaagctgcgg aatgtatagg ctgtca
//
LOCUS       DADLTY010000101        15096 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.101, whole genome shotgun sequence.
ACCESSION   DADLTY010000101 DADLTY010000000
VERSION     DADLTY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.101"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="JN334_002661"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="JN334_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HAY7438757.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="JN334_002662"
     CDS             complement(1151..1237)
                     /locus_tag="JN334_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HAY7438758.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="JN334_002663"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="JN334_002663"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAY7438759.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVGVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="JN334_002664"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="JN334_002664"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HAY7438760.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="JN334_002665"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="JN334_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HAY7438761.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="JN334_002666"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="JN334_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HAY7438762.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="JN334_002667"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="JN334_002667"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAY7438763.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="JN334_002668"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="JN334_002668"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAY7438764.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="JN334_002669"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="JN334_002669"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAY7438765.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="JN334_002670"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="JN334_002670"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAY7438766.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="JN334_002671"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="JN334_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HAY7438767.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="JN334_002672"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="JN334_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HAY7438768.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /locus_tag="JN334_002673"
     CDS             11797..13452
                     /locus_tag="JN334_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HAY7438769.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="JN334_002674"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="JN334_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HAY7438770.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..>15096
                     /locus_tag="JN334_002675"
     CDS             14575..>15096
                     /locus_tag="JN334_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438771.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAA"
BASE COUNT         3875 a         4031 c         3729 g         3461 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggattagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccgtc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca actcccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccattc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aggcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccctgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaacgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 gcccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgttcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caacgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggt ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cgctctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc tcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacaga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccaactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcc
//
LOCUS       DADLTY010000102        15006 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.102, whole genome shotgun sequence.
ACCESSION   DADLTY010000102 DADLTY010000000
VERSION     DADLTY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.102"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..251
                     /locus_tag="JN334_002676"
     CDS             <1..251
                     /locus_tag="JN334_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAY7438772.1"
                     /translation="IVTALSGIQRTSMNSLASRNQYQKLWRHGWQQTQLRAISPPANW
                     QVNRMQTSQAGCVSISVTLVSPGGREGEMTRLHCPNCQ"
     gene            264..3632
                     /gene="recC"
                     /locus_tag="JN334_002677"
     CDS             264..3632
                     /gene="recC"
                     /locus_tag="JN334_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HAY7438773.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRLWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            3808..6696
                     /gene="ptrA"
                     /locus_tag="JN334_002678"
     CDS             3808..6696
                     /gene="ptrA"
                     /locus_tag="JN334_002678"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HAY7438774.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            6689..10231
                     /gene="recB"
                     /locus_tag="JN334_002679"
     CDS             6689..10231
                     /gene="recB"
                     /locus_tag="JN334_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAY7438775.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            10231..12057
                     /gene="recD"
                     /locus_tag="JN334_002680"
     CDS             10231..12057
                     /gene="recD"
                     /locus_tag="JN334_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HAY7438776.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(12119..13450)
                     /gene="argA"
                     /locus_tag="JN334_002681"
     CDS             complement(12119..13450)
                     /gene="argA"
                     /locus_tag="JN334_002681"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HAY7438777.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            13682..14935
                     /locus_tag="JN334_002682"
     CDS             13682..14935
                     /locus_tag="JN334_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAY7438778.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
BASE COUNT         3550 a         3786 c         4184 g         3486 t
ORIGIN      
        1 tgattgtcac tgcgttatcg ggtatccagc gaacatcaat gaacagtctc gccagcagaa
       61 accagtacca aaagctctgg cggcatggtt ggcagcaaac gcaactgcgc gcgatttcgc
      121 cacctgccaa ctggcaggtc aaccgaatgc agacatcgca ggcgggatgt gtcagcatca
      181 gcgttacgct agtttcaccc gggggcagag aaggcgagat gacccgcctg cattgcccga
      241 attgtcagta gtcaggagcc gctatgttaa gggtctacca ttccaatcgt ctggacgtgc
      301 tggaagcgtt gatggagttt attgtcgaac gcgaacggct ggacgatcct ttcgaaccag
      361 agatgattct ggtgcaaagt accggtatgg cgcagtggct gcaaatgacc ctgtcgcaaa
      421 agtttggtat tgcggcaaac attgattttc cgctgccagc gagctttatc tgggatatgt
      481 tcgtccgggt gttaccggaa atccccaaag agagcgcctt taacaaacag agcatgagct
      541 ggaaactgat gactctgctg ccgcaattgc tggagcgcga agactttacc ctgttgcggc
      601 attatctgac tgacgatagc gacaagcgaa aactgttcca gctttcctca aaagcggcgg
      661 acctgtttga ccagtatctg gtctatcgtc cggactggct ggcacagtgg gaaacaggac
      721 atttggttga agggctggga gaagcacagg cctggcaagc cccgttgtgg aaggcgctgg
      781 tggaatatac ccatgaactc ggacaaccgc gctggcaccg cgcgaatctc tatcagcgct
      841 ttatcgaaac gctggagtcc gcgacgacct gcccgccggg gttaccttcg cgcgtcttta
      901 tatgcggtat ttccgcgtta ccgcctgttt atctccaggc gctccaggcg ctgggtaaac
      961 atattgaaat ccatctcctg tttaccaacc cctgccgtta ttactggggc gatattaaag
     1021 atcctgctta tctggcgaaa ctgctgactc gtcagcgtcg acacagtttt gaagatcgcg
     1081 aattgccgct atttcgcgat agcgaaaatg ccggacagct ctttaacagc gatggtgaac
     1141 aggatgtcgg caactcgctg ctggcctcat gggggaagct tgggcgcgac tacatttatc
     1201 tcctttctga cctggagagc agccaggagc tggacgcttt tgtcgatgtg acgccagata
     1261 acctgctgca taacattcag tctgacattc tggaactgga aaaccgcgcc gttgcgggtg
     1321 tgaacatcga agagttttcc cgtagcgata acaaacgccc gcttgatcca ctggatagca
     1381 gtatcacatt ccacgtttgc catagcccgc agcgtgaagt tgaagtttta cacgatcgct
     1441 tgctggcgat gctggaggaa gccccgacac ttactccgcg cgacatcatc gtgatggtgg
     1501 ctgatatcga cagctacagt ccgtttattc aggcagtgtt tggtagcgca cctgcggatc
     1561 gttacctgcc ttacgccatt tccgaccgtc gtgcgcggca gtcacatccg gtactggaag
     1621 cgtttatcag cctgttatca ctgcctgaca gtcgctttgt gtcagaggat gtgctggcgc
     1681 tgctggatgt gccggtgctg gcggcgcggt ttgacatcac cgaagaaggg ctgcgttatt
     1741 tacgcctgtg ggtcaacgaa tccggcattc gttgggggat agatgacgac aacgttcgcg
     1801 agctggaact ccccgccacc ggacaacaca cctggcgatt tggcctgacg cgtatgttgc
     1861 tgggctacgc gatggagagc gcgcagggcg agtggcaatc ggttctacct tatgatgaat
     1921 cgagcggctt aattgcagaa ctggtggggc atctggcttc actgctaatg cagctaaaca
     1981 tctggcgtcg cgggctggca caggagcgtc cgctagaaga gtggttgccg gtttgtcgcg
     2041 atatgctcaa cgccttcttc ctgccggatg cggaaaccga agcggcgatg acgctgatcg
     2101 aacaacaatg gcaggcgatt atcgccgaag gtttaggtgc gcagtatggc gacgcggtgc
     2161 cgctgtcact attgcgtgat gaactggcac agcgtctgga tcaagaacgt atcagccagc
     2221 gttttctcgc cggaccggtt aacatttgta ctctgatgcc aatgcgttca attccgttca
     2281 aagtggtttg cctgctggga atgaacgacg gcgtttatcc acgtcagctt gcgccattgg
     2341 gctttgacct gatgagccag aaaccgaagc gtggcgaccg tagccgtcgc gatgacgacc
     2401 gctacctgtt cctggaagcg ttaatttccg cgcagcaaaa actctatatc agctatatcg
     2461 ggcgttccat tcaggataac agtgaacgtt tcccgtcggt actggtgcag gaactgatcg
     2521 actacatcgg gcaaagtcat tatctaccgg gcgatgaagc gctcaactgt gatgaaagcg
     2581 aggcaagggt aaaagcgcat cttacttgcc tccatacccg gatgccgttt gatccgcaaa
     2641 actaccagcc aggcgaacga caaagctatg ctcgtgaatg gctacctgcg gccagccagg
     2701 ctggtaaagc acattctgaa tttgttcagc cgctgccgtt taccttaccg gaaaccgtgc
     2761 cgctggaaac gctacaacga ttctgggcac atccggtgcg ggcgttcttc cagatgcgtt
     2821 tgcaggtgaa cttccatact gaagacagcg aaatcccaga caccgagcca tttattctgg
     2881 aaggacttag ccgttatcaa atcaatcagc agttattgaa tgcactggtt gagcaggatg
     2941 atgccgaacg cttgttccgc cgcttccggg cggcagggga tttaccgtat ggcgcttttg
     3001 gtgaaatttt ctgggaaaca cagtgccagg agatgcagca gcttgccgac agagtcattg
     3061 cctgtcgcca gccggggcag agtatggaaa ttgatctcgc ctgcaacggt gtgcagataa
     3121 ctggctggtt gccgcaggtg cagccggatg gcctgttgcg ctggcgtccc tctttattaa
     3181 gtgtggcgca gggaatgcaa ctttggctgg aacaccttgt ctactgtgcc agcggtggta
     3241 atggcgaaag tcgccttttt ctacgcaaag acggtgagtg gcgttttccg ccgcttgcag
     3301 ccgaacaggc tttgcattac ctctcacaac tgattgaggg gtatcgtgaa ggaatgtccg
     3361 cgccattgct ggtgttacct gaaagtggcg gcgcgtggct aaaaacctgt tatgacgcgc
     3421 aaaacgatgc catgctggat gacgattcca cgttgcaaaa agcccgtacg aaattccttc
     3481 aggcttacga aggcaacatg atggtgcgtg gcgaaggtga tgatatctgg tatcaacggc
     3541 tctggcggca attaacacca gagacaatgg aggccatcgt tgaacagtcg caacgtttcc
     3601 tgttaccgct gtttcgcttt aatcagtcat gagtgttgta taaaaattgc gcaatctatc
     3661 tgcttacttt atgatgcgca ccagtcacgg actgatggtt atataaacat aggctgactc
     3721 gtgcagcaca agattaaatt ctggcagatg atttgcgtta acctgttgaa tctgtccaga
     3781 aaataaagtt gattatgagg tccgtgaatg ccccgcagca cctggttcaa agcattattg
     3841 ttgtttgttg ccctctgggc accattaagt caggcagaaa cgggatggca gccgattcag
     3901 gaaaccatcc gtaaaagtga taaagataac cgccagtatc aggctatacg tctggataac
     3961 ggtatggtgg tcttgctggt ttctgatccg caggcagtta aatcgctctc ggcgctggta
     4021 gtgcccgttg ggtcgctgga agatcccgag gcgtaccagg ggctggcaca ttaccttgaa
     4081 catatgagtc tgatggggtc gaaaaagtac ccgcaggctg acagtctggc cgaatatctc
     4141 aaaatgcacg gcggtagtca caatgccagc actgcgccgt atcgcacggc tttctatctg
     4201 gaagttgaga acgacgcctt gcccggtgcg gtagaccgcc tggccgatgc tattgcagaa
     4261 cccttgctcg acaagaaata cgccgaacgt gagcgtaatg cagtgaacgc tgaattaacc
     4321 atggcgcgta cgcgtgacgg gatgcgcatg gcacaggtca gcgcagaaac cattaacccg
     4381 gcacaccccg gttcaaagtt ttctggtggt aacctcgaaa ctttaagcga caaacctggt
     4441 aatccggtgc agcaggcgct gaaagatttc cacgagaagt actattccgc caatttgatg
     4501 aaggcggtta tttacagtaa taaaccgctg ccggagttgg caaaaatggc ggcggacacc
     4561 tttggtcgcg tgccgaacaa agagagcaaa aaaccggaaa tcaccgtgcc ggtagtcacc
     4621 gacgcgcaaa agggcattat cattcattac gtccctgcgc tgccgcgtaa agtgttgcgc
     4681 gttgagtttc gcatcgataa caactcagcg aagttccgta gtaaaaccga tgaattgatt
     4741 acctatctga ttggcaatcg cagcccaggt acactttctg actggctgca aaagcaggga
     4801 ttagttgagg gcattagcgc caactccgat cctatcgtca acggcaacag cggcgtatta
     4861 gcgatctctg cgtctttaac tgataaaggc ctggctaatc gcgatcaggt tgtggcggca
     4921 atttttagct atctcaatct gttacgtgaa aaaggcattg ataaacaata cttcgatgaa
     4981 ctggcgaatg tgctggatat cgacttccgt tatccgtcga tcacccgtga tatggattac
     5041 gtcgaatggc tggcagatac catgattcgc gttcctgttg agcatacgct ggatgcagtc
     5101 aatattgccg atcggtacga tgctaaagca gtaaaggaac gtctggcgat gatgacgccg
     5161 cagaatgcgc gtatctggta tatcagcccg aaagagccgc acaacaaaac ggcttacttt
     5221 gtcgatgcgc cgtatcaggt cgataaaatc agcgcacaaa ctttcgccga ctggcagaaa
     5281 aaagccgccg acattgcgct ctctttgcca gagcttaacc cttatattcc tgatgatttc
     5341 tcgctgatta agtcagagaa gaaatacgac catccagagc tgattgttga tgagtcgaat
     5401 ctgcgcgtgg tgtatgcgcc aagccgttat tttgccagcg agcccaaagc tgatgtcagc
     5461 ctgattttgc gtaatccgaa agccatggac agcgcccgca atcaggtgat gtttgcgctc
     5521 aatgattatc tcgcagggct ggcgcttgat cagttaagca accaggcgtc ggttggtggc
     5581 ataagttttt ccaccaacgc taacaacggc cttatgatta atgctaatgg ttacacccag
     5641 cgtctgccgc agctgttcca ggcattgctc gaggggtact ttagctatac cgctacggaa
     5701 gagcagcttg agcaggcgaa gtcctggtat aaccagatga tggattccgc agaaaagggt
     5761 aaagcgtttg agcaggcgat tatgcccgcg cagatgctct cgcaagtgcc gtacttctcg
     5821 cgagatgaac ggcgtaaaat tttgccctcc attacgttga aagaggtgct ggcctatcgc
     5881 gacgccttaa aatcaggggc tcgaccagag tttatggtta tcggcaacat gaccgaggcc
     5941 caggcaacaa cgctggcacg cgatgtgcaa aaacagttgg gcgctgatgg ttcagagtgg
     6001 tgtcgaaaca aagatgtagt ggtcgataaa aaacaatccg tcatctttga aaaagccggt
     6061 aacagcaccg actccgcact ggcagcggta tttgtaccga ctggctacga tgaatacacc
     6121 agctcagcct atagctctct gttggggcag atcgtacagc cgtggttcta caatcagttg
     6181 cgtaccgaag aacaattggg ctatgccgtg tttgcgtttc caatgagcgt ggggcgtcag
     6241 tggggcatgg gcttcctttt gcaaagcaat gataaacagc cttcattctt gtgggagcgt
     6301 tacaaggcgt ttttcccaac cgcagaggca aaattgcgag cgatgaagcc agatgagttt
     6361 gcgcaaatcc agcaggcggt aattacccag atgctgcagg caccgcaaac gctcggcgaa
     6421 gaagcatcga agttaagtaa agatttcgat cgcggcaata tgcgcttcga ttcgcgtgat
     6481 aaaatcgtgg cccagataaa actgctgacg ccgcaaaaac ttgctgattt cttccatcag
     6541 gcggtggtcg agccgcaagg catggctatt ctgtcgcaga tttccggcag ccagaacggg
     6601 aaagccgaat atgtgcatcc tgaaggctgg aaagtgtggg agaacgtcag cgcgttgcag
     6661 caaacaatgc ccctgatgag tgaaaagaat gagtgatgtc gccgagacac tagatccttt
     6721 gcgcttgccc ttacagggcg agcgcctgat tgaagcctct gccggcacag gcaaaacctt
     6781 tacgattgcg gcgctctatt tgcgcctgtt acttggacta ggcggttccg ccgcctttcc
     6841 ccgcccgctg atcgttgaag aactgctggt ggtcaccttt accgaggctg ccacggcaga
     6901 attgcgcggt cgtatccgta gcaatatcca cgagttgcgc atcgcctgtc tgcgtgaaac
     6961 caccgacaat ccactgtacg aacgcctgct ggaagagatc gacgataaag cgcaagccgc
     7021 gcagtggttg ttgttagccg aacggcagat ggatgaagcg gcagtcttta ctattcacgg
     7081 cttttgccag cgcatgctca acctgaatgc ctttgaatcc ggcatgctgt ttgagcagca
     7141 gctgattgaa gatgagtctc tgctacgcta ccaggcctgc gccgatttct ggcgtcgcca
     7201 ctgctacccg ctgccgcgtg aaatagccca ggtcgtcttt gaaacctgga aagggccgca
     7261 ggcgttgctg cgcgatatta atcgttatct gcaaggcgaa ccgccggtta tcaaagcacc
     7321 gccgcccgat gatgaaacgc tggcttcccg ccacgcgcaa attgtggcgc gtattgatac
     7381 ggtaaaacag cagtggcgcg acgcagtggg tgaactggat gcgctgatcg aatcttctgg
     7441 tattgatcga cgcaagttta accgtagcaa tcaggctaaa tggatcgaaa agatcagcgc
     7501 ctgggcagaa gaagagacaa acagttatca gttgccggag tcgctggaaa aattctccca
     7561 gcgtttctta gaagatcgca cgaaggccgg gggggaaacc ccgcgacatc cactgtttga
     7621 ggcgatcgat caactgcttg cagaaccatt gtcgatccgc gatctgttga tcacccgcgc
     7681 attggctgag atccgcgaaa cagtagcgcg tgaaaaacgc cgccgtggcg aattgggttt
     7741 tgatgacatg ttaagtcggc tcgattccgc gctgcgtagc gaaagcggcg aggtgttggc
     7801 agcggcgatc cgtacgcgat tcccggtggc aatgatcgat gaatttcagg ataccgaccc
     7861 ccagcagtac cgaatttttc gccgtatctg gcaccatcag ccggaaaccg cattgttgct
     7921 gattggcgac ccgaagcagg ccatatatgc attccggggt gcggatatct tcacttatat
     7981 gaaggcgcgt agtgaagttc atgcccacta cactttagac accaactggc gctccgcacc
     8041 tggaatggtg aacagtgtga acaagctttt cagccagact gatgacacgt tcatgtttcg
     8101 cgaaataccg tttattccag tgaaatcagc cgggaaaaat caggcgttac gttttgtatt
     8161 taaaggtgaa acgcagcctg cgatgaaaat gtggctgatg gaaggcgaaa gctgcggcgt
     8221 tggcgactat caaagtacca tggcgcaggt atgtgctgcg caaatccgcg actggctaca
     8281 agccggacag cggggcgaag cgttgctgat gaacggcgac gacgcgcgtc cggtgcgtgc
     8341 ttcggacatc agcgtgctgg tgcgcagccg ccaggaggcc gcccaggtgc gcgatgcctt
     8401 aacgttgctg gaaatccctt ccgtttacct ttcgaaccgc gacagtgttt ttgaaactct
     8461 ggaagcgcag gaaatgcttt ggttgttgca ggcggtgatg acgcccgaac gtgagaacac
     8521 cctgcgcagt gcgctggcaa cgtcaatgat ggggctgaat gcgctggata ttgaaacgct
     8581 gaataatgac gaacatgcgt gggatgcggt agtcgaagag ttcgatggtt atcggcaaat
     8641 ctggcgcaaa cgtggcgtta tgccgatgct gcgggcgctg atgtcggcgc gtaacattgc
     8701 agaaaacttg ctggcaacgg caggcggtga gcggcgtctt accgatatct tgcatatcag
     8761 cgaactgctt caagaagccg gaacgcagct ggaaagtgaa catgcgctgg tacgctggtt
     8821 atcgcaacat atcctcgagc cagacagtaa tgcctccagc caacaaatgc gcctcgaaag
     8881 tgataaacat ctggtgcaga ttgtcacgat ccataaatcg aaagggctgg aatatccgct
     8941 ggtctggttg ccatttatta ccaatttccg cgtccaggat caggcgtttt atcacgatcg
     9001 ccactcgttt gaggcagttt tggatcttaa tgctgcgcca gaaagcgtcg acctcgcgga
     9061 ggtcgaacgt ctggcggaag atctgcgttt gctttacgtg gcgctgacgc gttcggtttg
     9121 gcattgcagt ctcggcgttg caccgctggt gcgccgtcgt ggcgataaaa aaggtgacac
     9181 cgacgtccac caaagtgcgc tcgggcgttt gctgcaaaaa ggggaaccgc aagatgcggc
     9241 agggcttcgc acctgtattg aagcgttatg cgatgatgat attgcctggc aaacggcaca
     9301 aactggtgat aaccagccct ggcaggttaa tgatgcttta actgcagaac tgaatgcgag
     9361 gacactacaa cgattgcccg gcgataactg gcgtgtcacc agctactccg gcttgcagca
     9421 gcgtggtcac ggtatcgctc aggatctgat gccacggctg gatgttgatg ccgcaggcgt
     9481 ggtcagcgtc gttgaagaac cgacgttaac accgcatcag ttcccgcgcg gtgcgtcacc
     9541 ggggacattc ttgcacagtt tgtttgaaga cctcgatttt acccagccgg ttgacccgaa
     9601 ctgggtacag gaaaaactgg aactcggcgg ctttgaatcg cagtgggaac cggtgttgac
     9661 cgagtggatc acggctgtcc tccaggcacc tctcaatgaa acgggtgtta gcctgagtca
     9721 gctttccgac cgcgataaac aggtggagat ggagttttac ctgccgatta gcgaaccgct
     9781 catcgccagc cagcttgatg cactaattcg ccagtttgac ccgctatccg ctggctgccc
     9841 gccgctggag ttcatgcagg tacgcggcat gttaaaaggc tttatcgacc tggtattccg
     9901 ccacgaaggg cgttattacc tgctcgacta taaatccaac tggctgggtg aagacagttc
     9961 ggcttacacc caacaggcta tggcagcagc gatgcaggca caccgctatg atctgcaata
    10021 tcagctttat accctggcgc tgcatcgtta tctgcgccat cgcattgctg attacgacta
    10081 tgatcttcac tttggcggcg ttatctatct gttcctgcgt ggcgttgata aagaacatcc
    10141 gcaacaaggg atttacacaa cccgacccaa cgccgggttg attgccctga tggatgagat
    10201 gtttgccggt atgaccctgg aggaggcgta atgaaattgc aaaagcaatt actggaagct
    10261 gtggagcaca aacagctacg cccgctggat gtgcaatttg ccctgaccgt ggcgggagat
    10321 gaacatcctg ccgtcaccct cgcggcggca ctgttaagtc atgatgccgg agagggacac
    10381 gtctgtttgc cgctttcacg actggaaaat aacgaggcgt cgaatccgct gttggcgacc
    10441 tgtgtcagtg aaatcggtga gctacaaaat tgggaagaat gcttgctggc ttctcaagcg
    10501 gtcagcaggg gagatgaacc cacgccgatg atcctctgtg gcgatcgtct ttatttgaat
    10561 cgcatgtggt gtaacgagcg gacggtagcg cgcttcttca atgaggtgaa tcataccatt
    10621 gaggttgatg aagctctact ggcgcaaacc ctggacaaac tttttccagt aagcgatgaa
    10681 attaactggc aaaaagttgc agcggcagtg gcgctgacgc ggcggatctc ggtgatttcc
    10741 ggcggcccag gcaccggtaa aacgaccacc gtggcgaagt tgctggcagc gttaattcaa
    10801 atggccgacg gcgaacgctg ccgtatccgt ctggctgcac caacgggtaa agctgccgcg
    10861 cgcttaaccg aatctctcgg caaggctttg cgacagttac cgctgaccga tgaacaaaag
    10921 aaacgcattc cggaagatgc cagcactttg caccgattgc tgggcgcgca gccgggtagc
    10981 cagcgtttac gtcatcatgc cggtaacccg ctgcatcttg atgtgctggt ggtagatgaa
    11041 gcgtcaatga tcgatctgcc tatgatgtcg agactgatcg acgccttgcc agatcatgcg
    11101 cgagtgatct ttctcggcga tcgtgatcaa ctggcctcgg ttgaggctgg ggctgtgctg
    11161 ggcgatatct gcgcttatgc caacgcgggc tttaccgccg agcgtgccag gcagctaagc
    11221 cgcctgacgg ggactcacgt tccggcagga accggcacag aagcggcatc tttgcgcgac
    11281 agtctctgcc tgctgcaaaa aagctatcgt ttcggcagcg attctggcat tggtcagtta
    11341 gctgcggcga tcaaccgtgg tgataaaacg gcagtgaaaa ccgtttttca gcaggatttt
    11401 actgatatcg aaaaacgact tttacagagc ggcgaagatt atattgcgat gcttgaggaa
    11461 gctcttgcgg gttacggacg ttatctggat ctgctgcaag cgcgtgccga gccggattta
    11521 atcattcagg cgttcaatga gtaccagctt ttgtgcgccc tgcgggaagg gccgtttggc
    11581 gtggctggac tgaatgagcg aattgaacag tttatgcaac agaagcgcaa aattcatcgt
    11641 catccgcact ctcgttggta cgaaggccga ccggtgatga ttgcccgtaa tgacagcgcg
    11701 cttgggttgt ttaatggcga tatcggtatt gcgctggatc gcgggcaggg gacgcgcgtc
    11761 tggtttgcga tgccggacgg caatattaag tctgtgcaac cgagtcgctt gccagagcac
    11821 gaaactacgt gggcgatgac ggtacataaa tcgcagggat cggagttcga ccatgcggcg
    11881 ttgattttac cgagccaacg cacgccggta gtaacgcgag agctggtata caccgcggtg
    11941 acccgcgcgc gtcgtcgtct gtcgctgtat gccgatgagc gtatattaag tgcggcaatc
    12001 gccactcgta ctgagcggcg cagtggtctg gcggcgttgt ttagttcgcg ggaataaacg
    12061 taattgccgg atgcgacgca cgagtgttac gcctgccgca tccggtgatt ttcatcgctt
    12121 accctaaatc cgccatcaac actttggatt tacgctggta gttgtacaac tgctttttgc
    12181 tctcgggcag taaatcaata tccaccgggg taaatccacg ttcctggaac cagtgaatac
    12241 tgcgcgtggt cagcacaaac aatttgctta agccgctctg cttcgcctga gcggcaatgc
    12301 gttccagcag aacctcgccc cgtgatgaac tgcggtaatc cgggtgaact gccacacagg
    12361 ccatttcccc aatcttctct tccgggaacg gatagagcgc ggcgcaggca atagtcgtgt
    12421 tatcgcgctg aataatggtg aatttgtcga tttccatctc cagctgctcg cgagaacggc
    12481 gcaccagaat accttgctgc tccagcgggc gaatcaactc cagaataccg ccaatatcgt
    12541 tgattgttgc gcgacgaatc tgctcggcgc tttccatcac aatctgcgta ccgataccgt
    12601 cgcgtgagaa caactcttgc aacagcgcgc catcttcctg ataactgatt aaatgacagc
    12661 ggcgcacgcc gctgcggcag gctttcactg cgccacgcaa aaagcgcacc gtaccggagt
    12721 tgtaatcgcc tttctcttcc tgggcttcta cccgtgcttg cgcttcgtta gggaaaagtt
    12781 cggagacaat atcaccgtcg tcattagtaa cgccctggga agagcaaaaa ccaatcatct
    12841 tttcagcttt cagtttgatg gctagttgag tggcaatctc ttccgaggtc agattaaagc
    12901 tctcgccagt gactgaaaca gcgaccggcc ccattagcac tattgcaccg ctttccagtt
    12961 gacgatggag cgcgtcttca tcaatccgcc ggatacgccc gctatggcag taatccacgc
    13021 cgtcatcgac gcccagcggc tgggcaataa taaaattgcc actaacgacg ttgatatgcg
    13081 cgccctgcag cggcgtgtta ttgagactca ttgacaggcg agcagtaata tccagttgca
    13141 atgttcccgc agcctgcttc accagttcca gtgttttggc gtcggtcaca cgtatattct
    13201 tgtgatacag cggttcgtgg tgatgcgcag ccagatttgc gtcgatctgc ggacgtgcgc
    13261 catagaccac caccagacgg atgccgaggc tgtgcaacaa cccgatatca ttaacgatac
    13321 tggagaaatt ctcatgctca atggcttcac cgccgagcat gatgacaaac gtttttcccc
    13381 ggtgggtatt gatataggga accgaatggc ggaatccctc gaccagctcg gttttacgtt
    13441 cctttaccac ggcacacccc tttgcatgat tatttgaaat tattgtattt ttattctgtt
    13501 ttttcgcagg gtgcaagtgt aaattttatg cgagagcgac ttttttatca gtaatgccgt
    13561 gaatataaaa agaatgttta ccgtttatta gatgacagat tatgcgtctt tcgctaaagt
    13621 ttccggtcaa attagtcgtt tacttgttac acagcttaga tttcttttgc tcgggagagg
    13681 catgtcagga tccaacactg caatcagccg tcgtcgttta ctgcaaggcg cgggcgctat
    13741 gtggctattg agcgtaagtc aggtcagcct ggctgcggtt agccaggtcg tggcggtgcg
    13801 cgtctggcct gcgtccagct acacccgcgt gacggtagaa tcaaatcgtc agctgaaata
    13861 taagcagttc gcgttgagta accctgaacg cgtggtggtg gatatcgaag atgtaaacct
    13921 gaactcggta ctcaagggga tggcggcaca aattcgtgca gacgacccgt tcatcaagtc
    13981 ggcgcgcgtc gggcaatttg acccgcaaac cgtacgcatg gtttttgaat taaagcaaaa
    14041 cgtaaaaccg cagctgtttg cccttgcgcc ggtcgccggg tttaaagagc gtctggtgat
    14101 ggacctctat ccggccaatg cacaggatat gcaggacccg ctgctggcgc tgctggagga
    14161 ttacaacaaa ggcgacctcg aaaagcaggt gccgccagcg caaagtggtc cacaaccggg
    14221 taaagcaggg cgcgatcgtc cgattgtcat tatgcttgac cctggccacg gtggcgaaga
    14281 ctccggtgcg gtggggaaat acaaaacgcg cgaaaaagat gtggtattgc aaatagctcg
    14341 ccgtttgcgc tctctgatcg aggaagaggg caatatgaag gtgtacatga cgcgcaatga
    14401 agacatcttc attccgttgc aagtgcgcgt agcaaaagcc cagaaacagc gtgctgacct
    14461 gtttgtctct atccatgccg acgcctttac cagtcgtcag ccgagcggtt cctcggtgtt
    14521 tgcgctctca accaaaggcg caaccagtac tgcggcaaaa tatctggcac aaacccagaa
    14581 cgcctcggac ttgattggtg gcgtgagcaa aagcggtgac cgctatgtcg accacaccat
    14641 gttcgatatg gtgcagtcgc tgactattgc tgacagcctt aagtttggta aagcggtact
    14701 gaataagctc ggaaaaatca acaagctgca taaaaatcaa gttgaacagg ccgggtttgc
    14761 cgtactaaag gcaccagata ttccctccat tctggtcgaa acggcgttta tcagtaacgt
    14821 tgaggaagag cgtaaactga aaacggcgac tttccagcag gaagttgcgg agtctattct
    14881 tgcggggatt aaagcgtatt ttgccgatgg ggcaacgctg gcgagaaggg gatgataaaa
    14941 aggcgctgaa tggcgctttt tttatgggag atcagaaaca aaaaaacacc cgttagggtg
    15001 ttcaat
//
LOCUS       DADLTY010000103        14928 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.103, whole genome shotgun sequence.
ACCESSION   DADLTY010000103 DADLTY010000000
VERSION     DADLTY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.103"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            297..509
                     /gene="cspH"
                     /locus_tag="JN334_002683"
     CDS             297..509
                     /gene="cspH"
                     /locus_tag="JN334_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HAY7438779.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            951..1256
                     /locus_tag="JN334_002684"
     CDS             951..1256
                     /locus_tag="JN334_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438780.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1363..2007
                     /locus_tag="JN334_002685"
     CDS             1363..2007
                     /locus_tag="JN334_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY7438781.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            2004..2750
                     /locus_tag="JN334_002686"
     CDS             2004..2750
                     /locus_tag="JN334_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY7438782.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2750..4846
                     /locus_tag="JN334_002687"
     CDS             2750..4846
                     /locus_tag="JN334_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAY7438783.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            4892..6031
                     /locus_tag="JN334_002688"
     CDS             4892..6031
                     /locus_tag="JN334_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY7438784.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            6019..6465
                     /gene="etp"
                     /locus_tag="JN334_002689"
                     /gene_synonym="yccY"
     CDS             6019..6465
                     /gene="etp"
                     /locus_tag="JN334_002689"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HAY7438785.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            6485..8651
                     /gene="etk"
                     /locus_tag="JN334_002690"
                     /pseudo
     CDS             6485..8651
                     /gene="etk"
                     /locus_tag="JN334_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(8766..10064)
                     /locus_tag="JN334_002691"
     CDS             complement(8766..10064)
                     /locus_tag="JN334_002691"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HAY7438786.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(10144..10236)
                     /gene="cbdX"
                     /locus_tag="JN334_002692"
     CDS             complement(10144..10236)
                     /gene="cbdX"
                     /locus_tag="JN334_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HAY7438787.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10248..11384)
                     /gene="appB"
                     /locus_tag="JN334_002693"
     CDS             complement(10248..11384)
                     /gene="appB"
                     /locus_tag="JN334_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY7438788.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            complement(11396..12940)
                     /gene="appC"
                     /locus_tag="JN334_002694"
     CDS             complement(11396..12940)
                     /gene="appC"
                     /locus_tag="JN334_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HAY7438789.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(13074..13931)
                     /locus_tag="JN334_002695"
     CDS             complement(13074..13931)
                     /locus_tag="JN334_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HAY7438790.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13928..14326)
                     /gene="hyaE"
                     /locus_tag="JN334_002696"
     CDS             complement(13928..14326)
                     /gene="hyaE"
                     /locus_tag="JN334_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HAY7438791.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(14323..14910)
                     /locus_tag="JN334_002697"
     CDS             complement(14323..14910)
                     /locus_tag="JN334_002697"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HAY7438792.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
BASE COUNT         3778 a         3904 c         3760 g         3486 t
ORIGIN      
        1 ttattagaca tattttactt ccttcatttt gggcctttcg gcaaacaggg ctttaataca
       61 gaaaccactt agtgttcagg agagagactc aaaagaaggg gataatgcct gataatgaga
      121 actgctttag taaactactt tgtatgctgt ctgtctttca aaccgacgca gctattaagg
      181 catgataatt gagattaagc aaattttatt ttagccgtcc ggcggtctta aatatcgctg
      241 gctttatttt attaaccccg tatagtgcag cgcagtattt tcagtaagga atttgtttgt
      301 cccgtaaaat gacaggaatt gtcaaaacct ttgatcgcaa aagcggcaaa ggattcatta
      361 tcccctccga cggtcgtaaa gaagtccagg tccatatttc cgcattcact ccccgcgacg
      421 cagaagtgct tataccagga ttacgcgtgg aattttgccg cgtaaatggc ctgcgaggac
      481 caacagcggc aaatgtttat ctctcttaat atgacgctgc ttgtttaaag ctgactggtt
      541 ttattggaat agttcaatag tcaacaccat acattataac atcagttact tttccactaa
      601 gaaactaatc catttaagcg aatatctctg cacctaatag ttataaatgc catttaacag
      661 ccagttatag taccacttga aactatatta ccaacaataa ttaatagctt tcgtaacaat
      721 tttcttatct ttaattcgta tttttctaag atttatctta tgctcacaag tcgtatttcc
      781 agagggaaat cgttaacaat cttatatttc cctctgcgcg cctttcccac ccgcagtata
      841 tagtgctagc ggtatgagga aagggagatt tcacaaggat gttatactca aagtagacaa
      901 tcctgatctc aagcgtacgt attgtccatg cgatgaataa aggaaaagtt atgaaacaca
      961 agctttctgc aatccttatg gccttcatgt tgacgacgcc agctgcgttt gctgcccctg
     1021 aagcagcaaa cgggaccgag gcaacgacgg gtactactgg caccacaacg accactacag
     1081 gtgcaaccac gactgcggct accactagtg gcgtggctgc tggcgctgtc ggcacggcga
     1141 cagttgtcgg tgtggcaacg gctgttggtg tggcgacgtt ggcagtagtt gcagcaaatg
     1201 acagtggcga tggcggttct cataacacct caaccaccac gtccactacg cgctaattct
     1261 gcttaaacgc gtcgaaattc aagttaaata atacccggtt gcgatattcg taccgggctt
     1321 tagagatttg cattttttta attaaccttt caaggattaa aggtgcgccc tcttatttta
     1381 tcgattttcg cactatttct tgcgggatgt acgcacagcc agcaaagtat ggtcgataca
     1441 tttcgcgcca gccttttcga taatcaggat atcactgtag cggctcagca gatccaggcg
     1501 ttgccttatt ccacgatgta tttacgcctt aatgaagggc aacgaatctt tgtggtactg
     1561 ggatatatag aacaagaaca aagcaaatgg ttatcccagg ataacgccat gctggttacc
     1621 cacaatggac gtcttttaaa gaccgtcaaa cttaataata atctgctgga agtgacaaat
     1681 cccgggcggg cccctctgcg taacgcgctg gcaataaaag atggcagccg ctggacgcgc
     1741 gatattctct ggagtgaaga caaccatttt cgctctgcga ccctaagttc tactttttcc
     1801 tttgctggat tagagacgct gaatattgcg ggtcacaatg tgctgtgtaa tgtctggcag
     1861 gaagaggtga cttccacgct gccagaaaaa cagtggcaaa acacattctg ggtcgattcg
     1921 gctactggcc aggttcgtca aagtcggcaa atgttaggcg caggggttat tcccgtagaa
     1981 atgacgtttc ttaaacccgc accatgaata aattacagtc gtatttcatt gccagcgtac
     2041 tttacgtaat gacaccccat gcctttgcgc aaggaacggt gactatttat ctgcctagcg
     2101 aacaacagac actttccgtt ggccccgtgg aaaatgttgt ccagttggtg acacaaccgc
     2161 aactgcgcga tcgcctctgg tggcctggtg ccttgctgac cgactctgcg gcgaaagcta
     2221 aagcgcttaa agactaccaa catgtcatgg cacagcttgc ttcctgggaa gcagaagctg
     2281 acgacgatgt ggcggcaacg attaaatccg tgcgccagca gttactcaat ctcaatatca
     2341 ctggtcgctt accggtcaaa ctggaccctg attttgtacg agtggatgag aacagcaatc
     2401 ccccgttggt cggggactat acgctctata ccgtacagcg tccggtgact attactctgc
     2461 tgggtgcggt ttctggggct gggcaattgc cctggcaggc aggccgtagc gttaccgatt
     2521 atctgcaaga tcacccccgc cttgcaggcg cagacaagaa taacgttatg gtgattacgc
     2581 ctgagggtga gactgtcgtc gcccctgttg ccctgtggaa taaaagacat gtggaaccac
     2641 cgcctggcag ccagctttgg ttgggctttt ctgcgcatgt actgcccgaa aaatatgctg
     2701 atttaaatga ccagattgtg tctgttttaa cgcaacgtgt tcctgactga tgaagaagaa
     2761 ttcttatctt ttaagctgcc tggccattgc cgtctccagt gcctgtcatg ctgaagtatt
     2821 aacctacccg gatccgctgg gttcgtcgca atcagacttt ggcggcacag gattgttgca
     2881 gatgccaaat gcgcgcatcg caccggaagg tgaattcagc gtcaactacc gggataacga
     2941 tcaataccgg ttctactcca cctccgtggc gctgttccca tggctggaag gcaccattcg
     3001 ttatacggat gtgcgcacac gcaaatatag ccagtgggaa gatttcagcg gcgatcagtc
     3061 atacaaagac aaatcattcg attttaaact tcgcctgtgg gaagaaggtt actggctacc
     3121 gcaagtggcg tttggtaaac gtgatattgc tggtacgggt ttgtttgacg gtgaatatct
     3181 ggtggccagc aagcaagcgg ggccatttga tttcaccctc gggatggcat ggggctacgc
     3241 cggtaatgcg ggcaatatta ccaacccgtt ttgccgggtg agcgataaat attgtcatcg
     3301 cgcagagtct cacgatgcgg gcgatatcag ctttagcgat atctttcgtg gcccggcttc
     3361 catctttggc ggcattgagt atcaaacgcc gtggaatccc ctgcgtctga aactcgaata
     3421 cgacggcaac aattaccaga atgatttcgc tggcaaactg cctcaggcaa gccatttcaa
     3481 cgtcggtgca gtttatcgcg ctgccagctg ggcagacctc aacctcagtt atgaacgcgg
     3541 aaacacgttg atgtttggtt tcactttacg gaccaatttc aacgatctgc gccctgccct
     3601 acgcgatacg ccaaaaccgg cctggcagcc tgcgccagag tctgaaggtt tgcagtacac
     3661 cacggtagca aaccaactta ccgccctgaa gtataacgcg ggctttgacg cgccagaaat
     3721 tcagctacgc gataagacgc tgtatatgtc tggtcagcaa tacaaatacc gtgactctcg
     3781 cgaagcggtc gatcgtgcca accggattct ggtgaataac ctgccgcaag gcgttgagaa
     3841 gattagcgtg acgcaaaagc gcgagcatat ggcgatggtg actaccgaaa ccgacgtagc
     3901 cagcctgcgc aaacagctgg ccggcacagc gcctggtaaa tcagagcaac tgcaacaaca
     3961 acgtgttgaa gcagaagatc tttctgcctt tggtcggtgc taccgtattc gtgaagatcg
     4021 ctttagctac tctttcaacc caacactttc acagtcgctg ggcggcccgg aagatttcta
     4081 tatgttccag ctggggctga tgtccagcgc ccgctactgg tttaccgacc acctgctgct
     4141 tgatgacggt attttcacca atatttacaa caactacgac aagtttaagt cttcactgtt
     4201 acccgcggac tctaccctgc cccgcgtgcg cacgcatatc cgtgattacg ttcgcaatga
     4261 cgtttatctc aacaacttgc aagcgaatta ctttgccgac ttaggcaatg gtttctatgg
     4321 ccaggtgtat ggcggttatc tggaaacgat gtatgccggt gtcggttccg agcttcttta
     4381 tcgcccgcta gatgccagct gggcgctggg tgtggacgtt aactacgtga agcagcgtga
     4441 ctgggacaac atgatgcgct tcaccgatta ttccacgccc acaggtttcg tgacggctta
     4501 ctggaacccg ccgacgctca atggcgtact gatgaaactt agcgttgggc aatatctggc
     4561 aaaagataaa ggcgcaacga tcgacgtcgc caaactcttt gacagcggcg tggcggtagg
     4621 ggtatgggcg gcaatcagta acgtatctaa agatgactac ggcgaaggcg gctttagtaa
     4681 aggtttttat atctcgattc cgttcgactt gatgaccatt ggacctaacc gcaaccgcgc
     4741 ggtggtttcg tggacaccat tgacgcgtga tggtggacaa atgctgtcac gcaaatacca
     4801 gctcgatcca atgacggcag agcgagaagt accggttgga caataacctg acgtaattca
     4861 taacattaca ttgactgata aatataaacc aatgaaaaag aacattttta agttttcggt
     4921 actgacattg gctgttctgt ctttgacagc atgtaccctt gttcctggtc agaatttatc
     4981 gaccagtaac aaggatgtca ttgaactacc tgacaatcag tatgacctgg acaaaatggt
     5041 caatatctat cccgtcacgc caggattgat tgatcagctg cgcgccaaac cgattatgtc
     5101 gcaggctaac ccggagctgg agcagcaaat cgcaaactat gaatatcgta tcgggattgg
     5161 tgatgtgttg atggttacgg tctgggatca cccggaacta accacccccg cagggcaata
     5221 ccgtagcgcc agtgataccg gcaactgggt taacgccgat ggtgcgattt tctaccccta
     5281 tattggtcgc ctgaaagtgg ccgggaaaac acttacccag gtacgcaacg agattacagc
     5341 gcgtctcgat tcagtcattg aaagcccgca agtcgatgtc agcgtcgcgg cgttccgatc
     5401 gcaaaaagcg tacgtcaccg gcgaagtcag caaatcgggt cagcagccga ttaccaatat
     5461 tcctctgacc atcatggatg cgattaacgc cgcgggcggt ctgaccgcag atgcagactg
     5521 gcgcaacgtg gtgctcaccc agaatggcgt aaagaccaaa gtgaacctct atgcgctgat
     5581 gcagcgtggc gacttgcgcc agaacaaact gctgcacccg ggcgatattt tatttattcc
     5641 gcgcaacgat gatctgaaag tcttcgtgat gggtgaagtg ggtaaacaga gcacgctgaa
     5701 aatggaccgc agcggtatga ccctggcaga agcgctgggt aacgctgagg gtatgaatca
     5761 ggatgtcgct gatgcgacgg ggatttttgt tattcgtgca acgcaaaaca agcagaatgg
     5821 caaaatcgcc aatatttatc agctgaatgc gaaggacgca tcggcaatga tactcggaac
     5881 ggaattccag cttgaacctt acgacattgt ttatgtcact accgcgccgc tggctcgttg
     5941 gaaccgtgtg atttctctgc tggtaccgac gatttccggg gtgcatgacc tgacagagac
     6001 ttcacggtgg attcaaacat ggcccaacta aaatttaact caatcctagt ggtttgtacc
     6061 ggcaacattt gccgttcgcc cattggcgag cgcttgctgc gtaaacgtct gccgggcgta
     6121 aaagtcaaat cggcaggtgt tcatggtctg gtaaaacacc ctgcagatgc gacagcggca
     6181 gatgttgccg ctaatcatgg cgtatctctt gaaggacatg ccggacgtaa gctcactgct
     6241 gagatggcac gaaattacga tctgattctg gcaatggagt cggaacatat tgctcaggtt
     6301 accgcaattg ctcccgaggt ccgcgggaag acaatgctgt ttgggcaatg gctggaacag
     6361 aaagagatcc cggatcccta tcgtaaaagt caggacgcat ttgaacatgt ctacagtatg
     6421 ttggagcgcg ccagtcagga atgggcgaag cgcctcagcc ggtaatttga gtttataaaa
     6481 tacgatgaca actaaaaata tgaatacgcc accaggcagc actcaggaaa atgagatcga
     6541 tctgcttcgt ctggtcggcg agttatggga tcaccgtaag tttattatca gcgtgaccgc
     6601 gttattcacg ctgatcgctg ttaagcacac caatttatca ggcagatact ctggtccagg
     6661 ttgagcaaaa acagggcaac gccattctca gcggcctgag cgatatgatc cctaactcat
     6721 cgcccgagtc tgcaccggag atccaactgc tgcaatcgcg catgattctc ggtaaaacca
     6781 ttgctgaact gaatctgcgc gacatagttg agcagaagta ttttccgatt gtgggtcgcg
     6841 gctgggcgag attaaccaaa gaaaaactag gtgagctggc gatcagctgg atgcatattc
     6901 cacaactgaa tggtcaggat cagcaactga cactcacggt tggggaaaac ggccactata
     6961 cactggaagg tgaagagttc accgtcaatg gtatggtcgg ccagcgtctg gaaaaagatg
     7021 gcgttgcgct gactatcgcg gacattaagg ccaaaccagg aacacagttt gtcctgagcc
     7081 agcgtaccga actggaagcg attaacgcat tgcaggaaac ctttaccgtt agcgaacgca
     7141 gtaaagaaag cgggatgctg gaacttacca tgactggtga tgatccccag ttgattactc
     7201 gtattctgaa cagcatcgct aacaactatt tgcaacagaa tatcgctcgc caggcggcgc
     7261 aggattcaca aagccttgaa ttcttacagc gccagttacc tgaagtgcgc agcgagctgg
     7321 accaggcgga agaaaaactc aacgtttatc gccagcagcg cgattcggtt gaccttaacc
     7381 tggaagccaa agccgttctt gagcagattg tgaacgttga taatcaactc aatgagctga
     7441 ccttccgcga ggcagagatc tcccagctgt ataagaaaga tcacccaact tatcgtgcgc
     7501 tgctggaaaa acgccagacg ctggagcaag aacgcaaacg cctgaataag cgggtatcgg
     7561 caatgccttc cacccaacag gaagtgttgc gtttaagtcg tgacgtagaa gcgggccgtg
     7621 cggtatatct gcaattactt aaccgccagc aggagttgag tatttcgaaa tccagtgcca
     7681 ttggtaacgt gcggattatc gacccggcag tcactcagcc gcagccagtg aaaccgaaaa
     7741 aagcgttgaa tgtggtgctt ggttttattc ttggcctgtt tatttcggtg agtgccgtgc
     7801 tggcgcgtgc gatgttgcgt cgtggtgtag aagccccgga acaactggaa gagcacggca
     7861 tcagcgttta ttccactatc ccaatgtccg agtggctgga taaacgcacc cgtctgcgta
     7921 agaaaaattt attttctaat cagcagcgcc atcgtactaa aaatatcccc ttcctggcgg
     7981 tggataaccc ggcggattct gctgtggaag ccgtacgtgc gctacgaacc agtctgcatt
     8041 tcgctatgat ggagactgag aataacattc tgatgatcac cggtgcgacg ccagacagtg
     8101 gtaaaacgtt tgtcagttca actctggcag cggtgatcgc ccagtccgat caaaaagtgt
     8161 tatttattga tgccgactta cgccgtggtt attcgcataa cctgtttacc gtgagtaatg
     8221 aacatggctt gtcggaatat ctggcaggta aagatgagct caacaaagtg atccagcatt
     8281 ttggcaaagg agactttgat gtgattactc gcggtcaggt gccacctaac ccgtctgaac
     8341 tgctgatgcg cgatcggatg cgtcaattac tggaatgggc gaacgaccat tacgatctgg
     8401 tgattgtcga tacgccgccg atgctggcgg tgagcaatgc tgcggtcgtg ggacgctctg
     8461 tcggcaccag cctgctggtt gcgcgttttg gcttgaacac cgccaaagag gtgagcttgt
     8521 caatgcagcg tctggaacag gctggcgtta atattaaagg cgcaatcctc aatggtgtga
     8581 ttaaacgcgc cagcaccgct tacagttacg gctataacta ttacggttat agttactccg
     8641 agaaagagta aaactgaaag tgaccggatg cggcgtgaac gccttttccg gtctacgcat
     8701 tagacagttc ttcgtttgtc atcagcctca gagcattcag gtaactgaat gctctttttt
     8761 atgcattaca aactgcacgc cggtatgcgt gcttcattca cgatttgcgt aaaacctgcc
     8821 aacgaacaca tgccctgcgc atttcgctct tcacatcctg ccagggtcag tttcacctct
     8881 ccgggcggcg tatttaatga cagcggcgtt ttatcacgca tctgctgtaa agtctggaag
     8941 accagcgaaa cctgaatcca ctggctgtta tcgcttagcc gacgccagcg ttcaaacacc
     9001 agttcaccac ctggcggcgt gttatccggc tgacctggaa gcgtccagtt gagctccagt
     9061 gcgccgccga gatttgccag attagtatcg tgtccggcaa taaacagcac tgaagtgggt
     9121 aatgtcacac catacgcctg tttttgcggt ggatggggcg tcaacgctgc catgatcaaa
     9181 tccaataacg gggtggcgcg actgcgggca acctccggcg tgcgttgtag taaataaaat
     9241 tgcgcgttat gcaaacttag caaggtgttc cactggtgtg aatcggtgat ccttccccac
     9301 cccggctccg gcattccttg tgcctgttgc aggagaaata tctccgtcag cattgatgcg
     9361 aggcttaccg caccggttaa tgagacattg tcggcgctca ccttgagttc cgatggtaat
     9421 gcctgcgtta atgaacagct ttcgtcctgt ttctcacggt taaggcacaa gtttgattgc
     9481 gggaaattaa gcacccgttc cagttcgcga aacaccgttt gccgatgccc ggtaaagtca
     9541 gcaattgacc ctcctgccct gcagaggatc gcgtcagtca cgttcgcgtt atccagttgg
     9601 caaacaccag tttttagagg attaaataac ggatcgggac tggacgtatc tgcctgggta
     9661 tgtacggtta ttgcacagtc aggtgccagc ccggcggcga aggcttcgcc tgttttacgg
     9721 gtacgctcgt cgacatcagc aataatcgcg acctgagcag actgcgggca gccctttttc
     9781 gccagcaatc cgtcggctac cagacgctgg cgttggtaat gtccgagata ggcgattagc
     9841 tcaccaccgc gcggtgtcag ccaacccagt tttaccggcc aggttggcca tgcgtctggg
     9901 gtgacatcct gcatcagttg cgtggccttg gttggagcac gcacaccatg acgactgaca
     9961 atcaccatac tttccagctt cagctccggc tcactctgag cgaatgcaga ttgcggggtt
    10021 aacggaatca gaagagataa aaatgggatt aagatcgctt tcatcgatat gtttccgctt
    10081 ttcatatcta acattattga ttgcctgatg cgatgctaat gcgccgcatc aggcctacgt
    10141 tcgttaactt ttcagacgcg ggtccagcca taccaacaca agggtggaga gcgaacacat
    10201 caacaaaatg ccgacgaacc aaagtaaata ccacatgttc ctgctcctta gtacaactcg
    10261 tttttgttac ggcggagagt ttctgttgtc atgcgtcccc acattttgta gtagctccag
    10321 agagtgtaga gcaacacaat gggcaaaaat atcagcacga ttaccaacat aatgctcagc
    10381 gtcagctgac tggaagtgct gtcccacaac gtcaggctgg agattggact cacacttgac
    10441 ggcatgacaa aggggaacag cgtgatgcct gccgtgaaaa tcacgccaaa ttgcatcaat
    10501 gatgccatca aaaatcccca acccgggcga ccacgataaa tcgccatcac cgtcagcaat
    10561 gggcagaaga accccagcag cgggaagatc cacaaaacgg gcgattcgac gaaattattc
    10621 atccaggcac caggtagcac tgccaccagt ttcattaacg gatgggaagg accgttagca
    10681 tcctgggcga gcagtacaaa gccatcaata ccgacccaca gccagtaacc tgccagcaaa
    10741 aagcacagca tcaccaacag tgcagcgcgt ttagtcgcca gctgtgaacg cagatgaatc
    10801 acaccaacag ttttcagttg taaccagacg ccaccttgca aaatcaccat cccaaggctg
    10861 agcaaaccac acaataaagg gaatggcgtc agcagttgcc agaagctgcc gagatactcc
    10921 acgcgtaatt gcggtgtgaa ggcaaacggc acgccgagca acaagttgcc gaaggcgata
    10981 ccgaagacta ccggcggcac cagactgccg atgaccagac cggcatccca cattttacgc
    11041 cagcgtgcat cggcaatttt tccgcgataa tcaaaggcca gcggacggaa gaacagcgag
    11101 cacagcacca ggatcatcgc cacataaaag ccggaaaacg ccgctgcata cactctgggc
    11161 caggctgcaa ataatgcccc acctgcgagg atcaaccaga cctgattgcc ttcccagtgc
    11221 gcaccaacgc tattaatcac tatccggcgt tcatcatcat tacgcgccac cagcggcagc
    11281 aaacagccga tccccatgtc aaatccgtcg gagatcataa agaccaccag gatcacgcca
    11341 atcagcagcc accagatgaa gcgcaatgtt tcataatcaa acatgatttt ctcctttacc
    11401 cctgttgctg cgtcggttgt tcactctgca tcgcgctcgg ccccagacgg gcatatttct
    11461 gcatcaggta gacttcggcg attaagaaca gggtgtaaag ccctacgatc atgatcagtg
    11521 agaaagccag ttgtcctgtg gttaaagcgg agtgcgcgga gtatgtcggt aagatgtcct
    11581 gtatcgccca cggctgacga ccaaactcgg tcataaacca cccggcttca atcgcaatcc
    11641 acggcaacgg caaactccag agcgccattt tcagcaccca gcgatgctgg tcgattttgc
    11701 cacgcagcgt ctggacaagc gcaatcagca tcaccagtag cagcagggaa ccacagccca
    11761 ccatgatgcg gaaactccag aataccggcg caacctgagg tatcgcgcca cgcatcgccg
    11821 cctggtactg tgcggctgtg acatgattca tatccggcgc atagcgggag agcaacatgc
    11881 cgtaaccaag gtcgccttcc agctcccgga atgcctgcaa cacatgcggc tcacgattgc
    11941 cttgagatat ttcctgcatt aacagccagg ccatacgtcc gcgttgcaag cgtgggtagg
    12001 tttcagccat caaattcttc agacccggca cgggtttatc taatgagtga gtggcgagga
    12061 tccctagcag cgcgggaatt ttgatggcaa aggcgttacg ctcttgatcc tgttccggcc
    12121 aggcaaccac atggaacggt gcaggagcag gttccgtttg ccactcccct tccatcgccg
    12181 ccagttttac cggttgtact tgcgcgactt catacgcaga actgtctccg agttgcaggg
    12241 taccgataat cgccagagta ccgaagacgg aaccgatggc aaacgagcgt aatgcgacat
    12301 cgcgctcccg tccgcgcagt aaataccagg cgctgatcgc cataataaac atggccccgg
    12361 tcacgtagcc cgccatgacg gtgtgcacaa atttcacctg gctgaccggg ttaaagacca
    12421 gctcgctgaa gctggtcatc tccatacgca gggtgtcgat atcaaaatgc gcaccggtcg
    12481 ggtattgcat ccaaccgttg gcattcaata tccacaatgc agagagattt gaaccgaacg
    12541 ccaccagcca cgtcaccagc aggtgctggt atttattcag ccgttgccag ccgaagaaga
    12601 acagcccgac aaaggtggat tcgaggaaga aggccattaa tgcttccatc gccagtggtg
    12661 cgccaaaaat atcgcccaca tagttggaat agaatgacca gttagtacca aactgaaact
    12721 ccatggtcag gccggtagcc acgccaagag caaaattgat accgaagagc ttaccccaga
    12781 agcgcgtcat atcgcggtag attgttttgc cggtaaccac gtaaatggtt tccatgatag
    12841 ccagcaaaaa aatcagcccc agggtaaggg gtacaaataa aaagtgatac agcgcggtca
    12901 gagcaaactg ccagcgcgat aaatcaatga catcccacat gcgcactcct gtaggcgaag
    12961 agagaaaaat aacccgataa tgttagatct ccctctttat cctgaaactc tacgaccaga
    13021 ggtattcgcc gattgcgata ttcataagac tgaaagcgat acttaccgtc tttttacgtc
    13081 ggtgcagctt ccgccagcca ctgacatacc tcgctaagcc gctgcgcaga gtcgaccaaa
    13141 tcttctggcg ctgccagcac cacttccggt attgggcaga tttcataact ttccagtaac
    13201 gggcctttta aggtgtccgt acagcgtaaa tgccagacat ggcgtaaccc cgtggcgttg
    13261 atatagctct cgccatagcc aatggtacga atctgaatat ttcccggccc acagagacgc
    13321 gagagaaaaa gccgatcagc ctcgctgatg ggtaattgcg tcagattaat gctgtgcggc
    13381 tgcgcgtcgg ggttacgtac atgtgccagt aactcatgcg ccaacggtag gccattcatc
    13441 aggccatcaa tgggcggcgg taacagcgaa tctgtcggca agagattttg cgttgccgcc
    13501 tgccacaacg ccagcggcgc gcagccagcc tccagtttgt cctccagcaa cttttcgccg
    13561 cgacgtctgc gcacccgcca taatccgcag aagatcgcct cctgtatttc acttttactg
    13621 tcgtcagcct gctgaatgcg cactgacact tccccttccc cgagaagcgt attgataaag
    13681 tgacgatcgt cggcgttgag atttaacaga tcgactgcca gcacctcgcc caacggcggc
    13741 tggcgttcgc ttagttgatg ttgtaactcg tttaacagcg caatcacctg cgggctgtga
    13801 gcacattgct ccaaggccgc catactcggt tcatcattca cctgacaggt gatcggcagt
    13861 ggattcatgc tgaaactgtc atcgttcggt tgcgttcctg gccccagcag atggaaaaaa
    13921 gtttcgctca tgaggcacgc tcctgctgcg gttcgacaag cccgcgcatc aggtttatca
    13981 gttccgccca cgggtgaata ccattcagca cgccgcgata gtttccgccg gtaaacacta
    14041 aagtggcagg aaagcgaaag gcgccaaagc gatcgccgat ggcttcgctc tgctcaaggt
    14101 cagcaatcgc cacctgccat gtatagtcgg gaaactcgtg cagtaattcg ccaatcatta
    14161 ccggattatc gctgacctct ggcgtgcgtt tcgggtcact gcttaataac accacgccgt
    14221 ctggcgcttg cgtaagccag tcgtcaagac gggattcact gaccggcgtc cagccgcgcg
    14281 ccagcattcg ttgccacaac gcatcaaatg gcgtgtcgtt gctcatccct gctcctcctg
    14341 tgtcatccgg tactggcgca agcgaacgcc ttcgtaattt tccatcgaca gacagtcata
    14401 attgagacaa cgcgattcat tagccggttg cggcacactt ccccacgcag ccaactgcgc
    14461 cagcgccgat tgttccgcag cgggcagttg ctcccgtgcc agttcgctca ggctgccgcc
    14521 gtagtcgtcg agcatcgcgg gttgcagccc gacgagggca atatgtgctg gcagatgtcc
    14581 gcggatatct gccagcgcca gcacttcgga gaaactgttc tgatgcaggc tcattttctt
    14641 cgcgctgaga taggccggaa tgcgttctcc ggcataggtt cgcagcgttc caggttccag
    14701 cccgtagtca atggcatcga gaatcaacag atggctggcg ctttcgacat accccagcaa
    14761 gttcagtccc tgagtaccgc catcgacaat ctccacatac tcgggccagt ggtaatgggc
    14821 atacagccgt tccgccaccc gcacgccgaa gccttcatcg gcccacagca ggttgcccag
    14881 ccccatgacc accacgtgtt gctcgctcat gaacgctcct tgttactt
//
LOCUS       DADLTY010000104        14782 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.104, whole genome shotgun sequence.
ACCESSION   DADLTY010000104 DADLTY010000000
VERSION     DADLTY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.104"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            340..1698
                     /locus_tag="JN334_002698"
     CDS             340..1698
                     /locus_tag="JN334_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HAY7438793.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(1937..3322)
                     /locus_tag="JN334_002699"
     CDS             complement(1937..3322)
                     /locus_tag="JN334_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7438794.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(3372..3719)
                     /gene="tnpB"
                     /locus_tag="JN334_002700"
     CDS             complement(3372..3719)
                     /gene="tnpB"
                     /locus_tag="JN334_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7438795.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(3716..4096)
                     /locus_tag="JN334_002701"
     CDS             complement(3716..4096)
                     /locus_tag="JN334_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAY7438796.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(4451..4885)
                     /locus_tag="JN334_002702"
     CDS             complement(4451..4885)
                     /locus_tag="JN334_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438797.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(4873..5274)
                     /locus_tag="JN334_002703"
     CDS             complement(4873..5274)
                     /locus_tag="JN334_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438798.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(5534..6103)
                     /locus_tag="JN334_002704"
     CDS             complement(5534..6103)
                     /locus_tag="JN334_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAY7438799.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(6848..7024)
                     /locus_tag="JN334_002705"
     CDS             complement(6848..7024)
                     /locus_tag="JN334_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HAY7438800.1"
                     /translation="MAARNTSGDAGKVNSHTRYKLTLAELEAFNSAVDNRLAELTMNK
                     LYDRAPASVWKYVT"
     gene            complement(7325..7591)
                     /locus_tag="JN334_002706"
     CDS             complement(7325..7591)
                     /locus_tag="JN334_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438801.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            7732..7938
                     /locus_tag="JN334_002707"
     CDS             7732..7938
                     /locus_tag="JN334_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY7438802.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            8033..8635
                     /locus_tag="JN334_002708"
     CDS             8033..8635
                     /locus_tag="JN334_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438803.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            9835..10752
                     /locus_tag="JN334_002709"
     CDS             9835..10752
                     /locus_tag="JN334_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438804.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            10837..11709
                     /locus_tag="JN334_002710"
     CDS             10837..11709
                     /locus_tag="JN334_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HAY7438805.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            12081..>14782
                     /gene="ag43"
                     /locus_tag="JN334_002711"
     CDS             12081..>14782
                     /gene="ag43"
                     /locus_tag="JN334_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HAY7438806.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTIVQAGETVNGGTLTNHDNQIVLGTANGMTISSGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQTVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     HYTWQGLTLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSTK
                     HRVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNG"
BASE COUNT         3515 a         3893 c         3897 g         3477 t
ORIGIN      
        1 gcctttccag caatcgtcga ttgttatacc agtccaccca cgtgagtgtg gtcagctcat
       61 catactgaca cgtgcattac ccctaccgcg ataatgcaac cgtacaatat agggcgtttc
      121 ctaaacgcag ccctcatcac tccaatccaa caagacacag catttttgat cacgaagaaa
      181 gacaaataat ttccgtaaat gtttccgttg ttacaaataa caacagtgtt ttacatttct
      241 ttggcctcag gcccgcgcat gttgccattc gatgtcattt tcctgactcg tttcttcttt
      301 atggtgtagt gtgatcttca ctttatagat gctaacaata tgaaaagaaa aattattccc
      361 gtgctaatcg gttgtacgct ctctttctct gccctggcgg cacaacctac ggctgagcgc
      421 tatgtcgtca gttttccaga aggcacgcac gtgaactacg ccggcgcatt tgccagtgca
      481 ttcccgaacg gccttccggt tgggataggt tctggcctgc tgttcaccgg taaacagggc
      541 gatgccctga cattcgcaac cattaccgat cgcggtccga acgcggattc accgaaagag
      601 gggaaaaacg aaactaaaat tttcgttacg ccagatttcg cgccgctgct gatgacgatt
      661 cgtgtacaaa acggcaaagc ggaggccatc gatccccgtc ctctgcatga cgataaaggc
      721 gcgatcaacg gtctgccgct ggcaagcgac gtgattggct ccaccaatga agtggccttc
      781 agcgacacgt tacatagact gaagggggat aatcgcgggc tggatacgga agggatcact
      841 ccggatggta aaggaggtta ctggctgtgc gatgagtatg gcccgttcct gattaacatc
      901 gacagtaaag ggaaaatcct ggcaatccac ggtccgcagg cggcggaagg ggaaaaagcg
      961 attgcgggcg gtttgccaaa tatcctcaaa tggcgtcagg cgaaccgcgg ctttgaaggc
     1021 ctcacccgta tgccggacgg gcgtattatt gtcgccgtgc aaagtacgct ggatatcgac
     1081 gctaagagca agaaaaaggc gctgtttacg cgtctggtga gcttcgatcc ggcaagcgga
     1141 aaaaccgcga tgtacggtta tcctatcgac agcgcggcct acagcaaaaa cagcgacgct
     1201 aaaatcggag acatcgtggc gctcgataac cagcacatcc tgctgatcga acagggaaga
     1261 gataaaaaca acagaatgcg taacctgatt tacgaggtcg atctcaataa ggcgagcgac
     1321 ctgtcaggct tcgacaagcc gggtgagtat ccggagtttg atgatgagaa aaccctgtca
     1381 cagcgcggca ttacccttgc gcagaaaacg caggttgttg atctgcgctc cttgggctgg
     1441 cagcaggaga aggctgaagg gttggcgctg atcgacagca aaacgctggc ggtggcgaac
     1501 gataacgact ttggcgtgaa ggtggcgatg caacatccgg tcgagggcaa gacattcaag
     1561 gattatcggg tgaacgcgga aggaaagctg acgcttgacg acaagcaggt tgaaacgacc
     1621 ctcagagtga agccactgga gaaaccggag tctgacagtg aactgtggat tgtgacgctg
     1681 ccggaagcgt tgaaataagt ttataaggta cggacagcag tatcgcttag tttttcaaat
     1741 tcatgtatgt gaacaatatt ggggttccgg cgtagacccc caatgaaagc gtcaccacgt
     1801 gaaaacatgc ctgcacggtt tacacgtcat cccatggtgg cagccggtta actttcctgc
     1861 gccaccagtg gaaaaaggcc ataagcagtc cgaacaaaag taagcgtaca gcgagggccg
     1921 tattgacggg gatgtgttat tcagctggca gtgctatgcg ccacggaagc agttcgctga
     1981 cccggttgac cggccagtct gctatgacgc caagcacatg gcgaaggtag ctttctggat
     2041 ccacgtcatt cagtttgcac gtcccgatca ggctgtacag tagcgctccc cgctcaccac
     2101 catggccaga gccgaagaac agggtcttca tcttttcacg caaccagctt tccagggatt
     2161 tcaacagcgg tttcgttttt cgctgacgtt cagcaagccg ctgctctgcc ggcattcccc
     2221 ttatatccgc ctctatggcg tacaactgac cgatctgctc cagggcttct tccgtcagtg
     2281 ctgacgggat gcggacgtgc acatcgtgga tctttcggcg ggcatgagcc cagcaggcag
     2341 cttccgttat cccaccattg cgatacagct cgttgaaccc ggcgtacgca tccgcttgca
     2401 gcacaccgct gaagcaagca agatgagtct gcggatggat gctttttctg tccgggctgt
     2461 aagcgaacca cactgcaggt gccaacgctg acccggcatt gcggtcatca cgaacatacg
     2521 cccacaaccg cccggtcttc gtcttcttat tacccggcag cagtacctgg accggggtat
     2581 catcggcatg gagtttgccg tcagtcatga catagccatg aagcgcctct tccagcggag
     2641 acagcagccg gcagcatgca tccacccagc ccgacagcag tgaacggctc agctccacac
     2701 cttgccggcc gtatatttct gactggcaat acagcggggt gtgctctgca tacttcgagg
     2761 tcagcacgcg ggccagcagc cccggtccgg cgataccccg ctcgatgggc cgcgaaggtg
     2821 caggtgcctg cacgatggta tcgcactgag tacaggcatg tttttcccgt accgtccgga
     2881 taacccggaa ggcgctacgc atcaactcca gctgttcggc ggtatcctcg cccagatagc
     2941 tcagtgaacc gccgcagttc gggcagcacg gcgccgcagg caacagtcgc ttttcgtcac
     3001 ggggtagtga ttcagggaac ggcttacggg tgcgggtctg acgcaacgga cgctgtactg
     3061 ccgggtcata caccctacca gtcagcgtat cgctctcttt ctgaagccgg ttcagatcgg
     3121 cttccatttg tgcgatacgg cgggagactt tttcggaacg actgccgaag ttcatccggc
     3181 ggagtttatc cagctgcgcc tgcagatggt ctatttcgcg ctcccggttg ctcagctttt
     3241 cctgcagggc gtggatcagc gcttcctgtt cggccaggcg ctgtttcagc aggaagatgt
     3301 cgtcagaaga gatgtcgttc ataagcccgt attttaccgg gcttattctg tgacaaccag
     3361 gataaagaga tttacagcat ggtcagggag gtcagcagcc gcttgggctg tcgccagtcg
     3421 ataccttcca gcagcatcgc cagctgcgcc tgcgtaagga acactttgcc atcacgggct
     3481 gacggccagg cgaagcgccc acgctccagc cgtttggtca ggaggcacag tccgtcgccg
     3541 gtggaccaca gcagtttaac ctgactgccg ctgcggcccc ggaaaatgaa aatatggccg
     3601 gacatgggat cgtctttcag cgccgtttgt actttcgcag ccaggccgtt gaagctattt
     3661 ctcatatcgg tgataccggc aaccagccaa attttggtcc cggaaggtaa cgggatcatc
     3721 gcttcagttc ctgtatcagc agagtcagga gcttttcgct gacattgcca ttgaagcgga
     3781 gcgtcccgtg ccggaacgtt acctcacagc tgatactgag ggtttccggg tcctctgcga
     3841 gcgattctgg ctgttcggca gctgcatcga gagtcacagg aagtagctgg gggctctctg
     3901 aagaaggtaa tagcagcttt ccctcgcgcc attgttgtcg ccatttgaac aacagattgg
     3961 cgttaatgcc attttcaaga gcaagttttg agatggatat cccgggttca caggaggcag
     4021 caacgagcag ctgtttaaat tcgggaggat aattagggca gccttttcgc ctgccgggag
     4081 tcacattttt ctgcatatct gacactttgg ttcccactac ttatttggtg gacaccactt
     4141 tgtctaattc gtcagattct gaccagacgg ttcaggctgt acgcttacca ctattttatc
     4201 cctgttgaat ttggttaagc cggtgcagac gcgccgggaa acgcacacct gaaccggcac
     4261 aataaaatac aatacaaccc cctgttactg gagatacgaa tggaatgacg aggcagcctg
     4321 tcagtcacag ggggctgttg gcataaaatc aacggaaagg ccaccaacac cggaattaag
     4381 ctccacagtc cccgcactgg caatccattc ccgggaaagg catcaccgca aactgctccg
     4441 gaggcaacca ttacagcctc tgtaacggat tatcagccct ggcatcttcc gacggcacct
     4501 cctgccgccc ccacgccctg ctgtataccc cggtaccgca gcgtgggcag cacttttccc
     4561 cctcataatc ttcgtcacac atcacacaaa accactttct caccttcacc gtataatccg
     4621 gaaccgtcat gccctgcaga tgcaaacgac gggcctcttt ctctgccggg tcaagccagg
     4681 tacatcgcag caggattttt tcagcgacag tcatctggtc atgttcaaac cacggtggtt
     4741 gtccgtcatt gccccgctga agtgttaacg ggcatccaca ggacacacag cacaaaacgt
     4801 cctgtcggtt gtgcttcgca gcttctttcg ccgtaacaaa atggcctttg ctgtcattcg
     4861 ccatataaca gttcagaatt cgcatatttc ctcctgagtt gtccggggaa tactgaatcc
     4921 tccggtctgg cagtgtgtgc agtactgctc cccataataa tcgtggtgac attgtctgca
     4981 gtgccagctg gctttacgca ccacgggtaa ggcatccggt acgaattgct gcagacgttt
     5041 aatcaactgt atttctctgc gctccggcct gacataaggg cactgttgac cgtgctctgt
     5101 cagcctgtcg tcagtgtgtt caaaccaggg aagttcagtt tcgtattgcg gatggtatct
     5161 gagtgcactg ccacacaagt ggcaggtgta gttagcataa ggtgcggcct gtgcagtacg
     5221 ggcgcccgtc agacgtccgt tgccatcaag agcgataaag gattttgcgt acatagtcat
     5281 atacctcagc cgccagacga cacgcaggca acagccgtcc cggtacgggc agcgtggtca
     5341 gggtgtgaat ggcggttcag ttaaaatctg gactggagtt tgggtaaagc aggctggttg
     5401 ctgttacagc actgtggttg tcacacaatg gaacactgac gtaaaaaaca gtcagtgggc
     5461 gagtggatac atcgttgccg ccagacaaca acccatactt ttggtttgcc ggatattggc
     5521 gcgccagtcg tgctcaactc cggctacagc caaaattgcg ctcaccggtg cagtgagctt
     5581 tggtatgctc cttcgccaga tagtcagcac gttccagcac ttgctgaaag ccggtgtcat
     5641 cattacgcgc cagccacacc gccggccagg ccggaaaatg caccagcgtg gcatggcacc
     5701 cgacatccac tcccagggca ctgcaccacg cctgtttaat catcccggcc agagactccg
     5761 gagcgcggta atcaccggca cgacaccagg tatcccggtt gaccagcagc atcaggtgat
     5821 agtgtttttt acctttgcac tccccaaact cccgggccca gacgtaatgc agggttgtgg
     5881 gatgcacgcg tttaccttcc cgatgcttac gtttctggta agcgtcgatt cgggctttca
     5941 gggcattgat gaagcgggat atcacagctg cgtccgttgc tgccggtaca tccgggagac
     6001 gcaggtcaac ccgcaatgcc gtcaggcggg gatgaacatt cagtgcgtgc cgcaccgtct
     6061 cacgaatacg ttgctgccag aaggggttgt atttgtaggt catggttaaa tctccgtatg
     6121 gttcatacgg aatagccacg tcgtaaaaaa tacgcagagt ccctgaagtg acctccggca
     6181 ggacactgtc tcaggcactc tgtgataacc cgaacggtcg tctccggact gacggttgaa
     6241 ttttcagcgt cgttttttaa tgtaaaaaac cggttacggg taaggctgtg aggagggagt
     6301 gatggtgata cgcaaaaaga agtgcaggga ctgtggaaac gcgattactc ataatacagt
     6361 gtgttgcccg tattgtggtt ctgtcgaccc cttcggctac taccggaata cagaccgtat
     6421 cgttaccata ctgctggcgc tgattattgt ggtcctgctg actactgtca gcgtcagtgt
     6481 gtgtatactg tgtagctggt gagataacgg ttcatgtatc ccctgagctg acagtgtacc
     6541 gtgtatatta agcgtaaccg acaggttctc cttcatacca tattaaattt tccagggatt
     6601 gccgctgcct tatttcgtcc catacaggaa aactcatctg ggtttctgac aaatacgcga
     6661 aactcacagc ccctcaatat caaccacacg ttgtaagccg gtaatacctg aaaatcagcc
     6721 tattaccgcc cacgcctccg gcaccattcc cttttgattt cgaattcttt gttgtcgcta
     6781 taccgcttaa tgttcccggt cctccggtgc agataacacc ggagtcatcg tagcgctgct
     6841 tcaatattca ggtgacgtat ttccagacgg aagccggcgc gcgatcgtaa agtttgttca
     6901 ttgtcagttc tgccagccgg ttgtcaacgg cagagttaaa ggcttccagc tccgcaaggg
     6961 taagtttata acgcgtgtgg gaattcactt ttcccgcgtc tcctgacgta ttacgggctg
     7021 ccacttacct ggtgtcccgc aatacagcct tagtgttccg ttatcgggga tccggacgca
     7081 ccgcaacaac cggggaagta tttctggttt agagcagtgt caagagggat ttctcagccc
     7141 ctcactcacc ggctcagtct cagggcagga gcattaatac gctcagttca tgtcttatac
     7201 aggaatgggc acagggggag ctggcgtgtc actgctgatt ttacgcatca gcgttcatgt
     7261 atctgcactc tgatacatga acgattcaca gcgctctgtt accggagaga tttactctct
     7321 tagcttactc tggcaaatcc tttacgttac tctctgatga cttcttatat ttataatacc
     7381 aatcagacag ccttatccca tcggtgatat cagtctcctc ctcgccagga acgggctgac
     7441 tcactgtaac tacctccgaa aacaactctg caattatcag aacgcctgct tctctccctg
     7501 tcctcacgaa aactatcccc tctttatcgc gcgtgcgtgc ggaagcatct tttcgcaaca
     7561 accacccggt ataccggtac ggctcagcca ccgcaacctc cccgtttatc tcctggcggt
     7621 cacattcccg attatttttt acgtttctcc ccggttgtta tgccggtgaa ggtggcgcgc
     7681 tgttttcatg accacaccgg ctggtttaac aacatccgga ggaacattct catggctacc
     7741 ccagtttcac tgatggatga ccagatggtc gacatggcat ttatcactca actgaccggc
     7801 ctgacagata agtggtttta caggctcatc agggatgggg cctttccggc tcccatcaag
     7861 ctgggccgca gctcccgctg gctgaaaagt gaagtggaag cctggctaca ggcgcgtatt
     7921 gcacagcccc gcccgtaatt tctgaccgtt atccgttcac ccgcagcaga cgtaatcccc
     7981 ggcctgccgg cggcattctg ctgcctgtta tatccccgtg aggaatatga aaatgaaaca
     8041 acagtatcag acccgctacg aatggctcca cgaaagctac cagaaatggc tgaccggctt
     8101 cacccggcac gccgtatcct ggggcgtgtg tcatccgaat atctactact tccataatct
     8161 gacgcccggt tgggtgtcat tcaatggtga aaaaccggag attgccatcg taccccagag
     8221 cctgcaccgg ctgatttatg gccctgacaa acgggccacg ccgcccctgg atgatgatct
     8281 gatagtgaat ttatgcacca gtgaacatct gctggttcat catccgatgc tggaaggcat
     8341 tctcctgtct gaatgtgaac gtctgaggca gcgttctctg gcaaataaac tgatcagcct
     8401 cttccgtcag tttggtggca cggagttgcg cctcaaactg gtctggcttt gctggcttga
     8461 tttaatgacc ggaaacagcc ttgaagactg gaaagagaac ctgaaacgga aatcagaaaa
     8521 agagctggag gaatggatca tcaaccgtca gaggcagagc gcagcactga cggatctgat
     8581 ggatcagtac gtgctcctgg cataccgcac aacggttgac gatagccgca actgatgccg
     8641 gttgcttccg gaaggtgtga caaaatacgg aaatgatgat gctcccggcc cccatgctca
     8701 cctgcagagc gtgttcgtcg tcatcatact gagccatgac gggcgtctta agggcatcct
     8761 gtcattgctg agcaggcaac accaccagag gagccacgga cagggatgtt ctttacagcc
     8821 cgatacacgc caggttctca gcagactgaa tgtgcaggtg cggttattca ccagaaatgg
     8881 catgctgcgg tacataccca tcctggtgac aatatgtacg tggcgacagc gatcccggca
     8941 ctgtggaaga gactcactca gcctgcagga tattctttcc ggcatccggg aatgtccgcc
     9001 tgaatgcaaa caggatgata tttgaatccg taatactgtt ctttcatcat aaataattta
     9061 tgcagataca gttaaccgtt acagtagccg gtttgtttta ccgggaaata atatccgtca
     9121 gactgatacc ggaacatatc tgaaacacgt tatgatgttt tgttatcaga aatgttgtat
     9181 attcttatcc gtggcgacag tttaatgccc tctggtatca cctgataatg tatatcctgc
     9241 gatgattcgt tatatttacg ggacacgcct cttttcattg tgctcctttt cagacttcat
     9301 tttccgtaag tgttccccgg cataaaggag gtacctttct ggcgtttata actctcttta
     9361 tcggtgacgc cggtaataca gggaatacca caccgacaaa cgaggtatta acagacgata
     9421 tttcagtgtc cataattttt ctgtccggtt aaataacgtc agtctgctgc cctctgactg
     9481 taatcatcaa agccggaatg cataccgggt tacaacgtaa aaaaagtgat gctggccccc
     9541 ggtgcagctg gcgggcgaaa catgtttacc tttcaatacc ccctgtctgc agtctccact
     9601 cttccatcct cttaagctac gtcggccaca tgagtgcact tccatgataa caggtattcc
     9661 tgtcagcata acgaagccgg ttaccttccc ttcctcctgc atttcctgtc agaatgacta
     9721 tacagcgcgc agattgcctt tttattgacc atcttaatat accgttataa acttcttttt
     9781 atcatattat tatcacctta ttatgtttaa tttttaagca gtcggagaaa gaggatggtt
     9841 cagaaaattt tgtcggataa ggttatgaat gaaagaacaa atgcgtatta ctcttattat
     9901 cttggagagc gtaatatctc cgtactgccc cttaatgttt atgatccccc ggaacgtttt
     9961 atcgcttaca taaaaaaaaa cagagaaaat ctgaacatca cactttcaga ttttgaactt
    10021 gaacaaataa tttctggtat gcgtctgaag gccctggcct tcctggtccc cctggaaaaa
    10081 atatcctgga tcgcgggcag tgaacgcgca tgcttatttt cctggtatct gttgatgcag
    10141 tttatccaga ataacagggc aaaaataagc gcagacttgc ttcaaaagaa taaactgtat
    10201 cttaaagaag agtatctgga agggaatgcc ttcccgtcgg actcatcgac acagttcaga
    10261 caaatactca gagttctcga tattctttct gataagaatt tgcgggatga gtggataatc
    10321 cagaccaaag acagatggat gcgggcattt aaatcaaaga gtcctttcag ttacctcctc
    10381 ccggagaacg aacatgaatg catatggaca tggaattatt tgaagggaaa aaatattgca
    10441 ctggaaaaac tggccagttt tcctggttct gctgatattt accacgcaat tcacctttca
    10501 tttgacatat gggttacctg tccactcacc tcacctgacg atataaaaaa tttcagaaac
    10561 agtttcaata aggccaaggc gcagcgcaag tacaaaaaaa tgcaggaaga taaagtcaat
    10621 gtacagtttt ttcttgacgc agaaaccagg gcgcagctca aagaattatc gagggtcaga
    10681 agactcagta ccggcgaaat gctgcatgac ctgattgttg aagagtataa aaggtaccga
    10741 cattccagat aatttcagac atatattatc tccgtgagtc ccccgccacc tttccggtgt
    10801 gcgggggttt tgtctttttt caccgggaat acatgtatga atccgtctga tgccattgag
    10861 gcaattgaaa aaccgctctc ctccctgcct tactcacttt cccgtcacat cctggaacat
    10921 ctgcgcaaac tcaccagtca cgaacccgtg attggcatta tgggtaaaag cggggccggt
    10981 aaatcctcac tctgtaatgc actgtttcag ggggaggtca ccccggtcag tgatgttcac
    11041 gccggcaccc gggaagtgca gcgtttccgt ctgagtggcc atggtcacag catggttatc
    11101 actgacctgc ccggggtggg cgagagccgg gacagagatg cagagtatga agccctgtac
    11161 cgtgacattc tgcctgaact tgacctggta ctgtggctga ttaaagccga tgaccgtgcc
    11221 ctgtctgtgg atgagtattt ctggcgacac atcctgcacc ggggacatca gcaggtgctg
    11281 tttgtggtga cgcaggccga caaaacggag ccctgccatg aatgggatat ggccggtatt
    11341 cagccttctc ccgcacaggc acagaatatt cgcgaaaaaa cggaggcggt attccgtctg
    11401 ttccggcccg tacatccggt tgttgccgta tcggcccgca ccggctggga actggatacg
    11461 ctggtcagtg cgctaatgac agcacttccc gaccatgccg ccagtcccct gatgacccgc
    11521 ctgcaggacg agctgcgcac ggaatctgtc cggggtcagg cccgtgaaca gtttaccggt
    11581 gcggtggacc ggatatttga cacggcggag agcgtctgtg ttgcctctgt tgcccgtacg
    11641 gttctgcgtg ccgtccgtga cacggtggtc tctgttgccc gcgctgtatg gaactggatt
    11701 ttcttctgaa cagtctgcca gggaaatgcc tgataacatt acgttaacca actgaaaaca
    11761 tgacacaaac ccctcatact cctcctgttt ctcttgcgtg accgccttgt cgttaataga
    11821 ataaaactat cgataaaaca gatatcgatc gttgctatcg atcgtttata gtggttaatg
    11881 gtctgcgaaa aggcagctga atctcttcat catgcagaac ggaattgcac acaacggact
    11941 gatacttctc tggcggatga gagaggggaa gcatttatgt ccgggaaaaa ccactttatc
    12001 tgaccatgct gttttgtacc tgccggtatc cacttttgtg ggtaccggct tttttattca
    12061 ccctctgtaa ggaaaagctg atgaaacgac atctgaacac cagctacagg ctggtatgga
    12121 atcacattac gggcaccctg gtggtggctt ccgaactggc ccgctcacgg ggaaaacgcg
    12181 ccggtgtggc ggttgtgctg tctcttgctg ctgtcacatc agtcccggca ctggctgctg
    12241 acacgattgt acaggcggga gaaaccgtga acggcggaac actgacaaat catgacaacc
    12301 agattgttct cggtacggcc aacggaatga ccatcagtag cggtctggag tatgggccgg
    12361 ataacgaggc caataccggc gggcaatgga tacaaaatgg cggtatcgcc aacaacacta
    12421 ctgtcaccgg tggtggtctt cagagagtga atgccggagg aagcgtttca gacacggtta
    12481 tcagtgccgg aggcggacag agccttcagg ggcaggcagt gaataccact ctgaacggcg
    12541 gtgagcagtg ggtacatgaa ggcgggattg caacgggtac cgtcattaat gagaagggct
    12601 ggcagaccgt caaatccggc gcaatggcaa ccgacacggt tgtgaatacc ggcgcggaag
    12661 ggggaccaga tgcagaaaat ggtgataccg ggcagtttgt tcgcggaaat gccgtacgta
    12721 ccactatcaa tgaaaatggt cgtcagattg tggctgctga aggaacagca aataccactg
    12781 tggtttatgc cggcggcgac cagacggtac acgggcatgc gctggatacc acactgaatg
    12841 gcggttacca gtatgtgcac aacggcggta cggcgtctgg cactgttgta aacagtgacg
    12901 gctggcagat tatcaaggaa ggtggtctgg cggatttcac caccgttaac cagaaaggca
    12961 aactgcaggt gaacgccggt ggtaccgcca cacatgtcac cctgaaacag ggtggagcac
    13021 tggttaccag tactgcggca acggttcttg gcagcaaccg tctgggcaat ttcacggtgg
    13081 aaaacggtaa ggctgacgga gttgttctgg agtccggcgg tcgcctggat gtactggaag
    13141 gccattcagc gcagaaaaca cgggtggatg acggcggaac cctggcagtg tctgccggtg
    13201 gtaaggcaac aggtgtcacc atgacatccg gtggtgccct gattgcagac agtggtgcca
    13261 ctgttgaggg gaccaatgcc agcggtaagt tcagtattga tggcacatcc ggtcaggcca
    13321 gcggactgct gctggaaaat ggcggcagct ttacggttaa tgccggagga ctggccagca
    13381 acaccactgt cggacatcgt ggaacactga cgctggctgc cgggggaagt ctgagtggca
    13441 gaacacagct cagtaaaggt gccagcatgg tactgaatgg cgatgtggtc agtaccggcg
    13501 atattgttaa cgcaggggag attcgctttg ataatcagac gacaccggat gccgcgctga
    13561 gccgtgctgt tgcaaaaggc gactccccgg taacgttcca taaactgacc accagtaacc
    13621 tcaccggtca gggtggcacc atcaatatgc gtgttcgcct tgatggcagc aatacctctg
    13681 accagctggt gattaatggt ggtcaggcaa ccggcaaaac ctggcttgcg tttacaaatg
    13741 tcggaaacag taacctcggg gtggcaacct ccggacaggg tatccgggtt gtggatgcac
    13801 agaatggcgc caccacagaa gaaggtgcgt ttgccctgag tcgcccgctt caggccggcg
    13861 cctttaacta caccctgaac cgtgacagcg atgaagactg gtacctgcgc agtgaaaatg
    13921 cttatcgtgc tgaagtcccc ctgtatacat ccatgctgac acaggcaatg gactatgacc
    13981 ggattctggc aggctcccgc agccatcaga ccggtgtaaa cggtgaaaat aacagcgtcc
    14041 gtctcagcat tcagggcggt catctcggtc acgataacaa cggcggtatt gcccgtggag
    14101 ccacgccgga aagcagcggc agctatggct tcgtccgtct ggagggtgac ctgctgagaa
    14161 cagaggttgc cggtatgtct ctgacgacag gggtgtatgg tgccgcaggc cattcttccg
    14221 ttgatgttaa ggatgatgac ggttcccgcg ccggcacggt ccgggatgat gccggcagcc
    14281 tgggcggata cctgaatctg acacacacgt cctccggcct gtgggctgac attgtggcac
    14341 agggaacccg ccacagcatg aaagcgtcat cggacaataa cgacttccgt gcccggggct
    14401 ggggctggct gggctcactg gaaaccggtc tgcccttcag tatcactgac aacctgatgc
    14461 tggagccaca actgcactat acctggcagg gacttaccct ggatgacggc caggataacg
    14521 ccggttatgt gaagttcggg catggcagtg cacaacatgt gcgtgccggt ttccgtctgg
    14581 gcagccacaa cgatatgagc tttggtgaag gcacctcatc ccgtgacact ctgcgcgaca
    14641 gtacaaaaca ccgtgtgagt gaactgccgg tgaactggtg ggtacagcct tctgttatcc
    14701 gcactttcag ctcccggggt gacatgagca tggggacagc cgccgccggc agtaacatga
    14761 cgttctcacc gtcccggaat gg
//
LOCUS       DADLTY010000105        14479 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.105, whole genome shotgun sequence.
ACCESSION   DADLTY010000105 DADLTY010000000
VERSION     DADLTY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.105"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(113..379)
                     /locus_tag="JN334_002712"
     CDS             complement(113..379)
                     /locus_tag="JN334_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HAY7438807.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="JN334_002713"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="JN334_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAY7438808.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4544..5673
                     /gene="ompN"
                     /locus_tag="JN334_002714"
                     /pseudo
     CDS             4544..5673
                     /gene="ompN"
                     /locus_tag="JN334_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5814..6248
                     /gene="uspF"
                     /locus_tag="JN334_002715"
     CDS             5814..6248
                     /gene="uspF"
                     /locus_tag="JN334_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HAY7438809.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6424..7359
                     /gene="ttcA"
                     /locus_tag="JN334_002716"
     CDS             6424..7359
                     /gene="ttcA"
                     /locus_tag="JN334_002716"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAY7438810.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="JN334_002717"
     CDS             complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="JN334_002717"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HAY7438811.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="JN334_002718"
     CDS             complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="JN334_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HAY7438812.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10571..11803
                     /gene="dgcM"
                     /locus_tag="JN334_002719"
                     /pseudo
     CDS             10571..11803
                     /gene="dgcM"
                     /locus_tag="JN334_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="JN334_002720"
                     /pseudo
     CDS             complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="JN334_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12641..14095)
                     /locus_tag="JN334_002721"
     CDS             complement(12641..14095)
                     /locus_tag="JN334_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY7438813.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14092..>14479)
                     /locus_tag="JN334_002722"
     CDS             complement(14092..>14479)
                     /locus_tag="JN334_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7438814.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3860 a         3651 c         3418 g         3550 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaacttctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat
     4381 acattcacca gacttattat catttcagag gaattatttg attaaggttt tacttaaggc
     4441 gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga
     4501 aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact
     4561 ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa
     4621 taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga
     4681 taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca
     4741 aattaacgat caacttactg gctacggtca atgggaatac aatattcagg caaacaacac
     4801 tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga
     4861 ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac
     4921 cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg
     4981 tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct
     5041 gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac
     5101 caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata
     5161 tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga
     5221 ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa
     5281 atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc
     5341 gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac
     5401 cagtttgatt tttgtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
     5461 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
     5521 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
     5581 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
     5641 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     5701 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     5761 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     5821 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
     5881 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     5941 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     6001 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     6061 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
     6121 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     6181 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     6241 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     6301 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
     6361 gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     6421 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     6481 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     6541 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     6601 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     6661 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     6721 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     6781 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     6841 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     6901 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     6961 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     7021 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     7081 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     7141 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     7201 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     7261 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     7321 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caggatagcg cccgatgcgc
     7381 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatctggca
     7441 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     7501 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     7561 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     7621 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     7681 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     7741 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     7801 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     7861 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     7921 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     7981 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgttgctc cagatcgccg
     8041 tgtaacgaca atgcactttg ctctacttca ttcagcgcgt cgcagacagc ctggcaatct
     8101 tttttggtat tgcaaaacac cacacaagag gatggctgat gcaagcttaa taaccgttgc
     8161 aacagaggaa ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa
     8221 gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg
     8281 gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa
     8341 cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc
     8401 atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa
     8461 cgacccggcg ttgccacgat aatatgcggc gcatgttgca gcgaatcacg ctgcataccg
     8521 aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga
     8581 cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg
     8641 gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg
     8701 ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg
     8761 gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg
     8821 ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc
     8881 aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt
     8941 cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa
     9001 caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat
     9061 ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa
     9121 aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa
     9181 aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag
     9241 ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt
     9301 ttgtatacag acagccggaa aaattgcttt tgttacaacc atttactacg gtgcaaccat
     9361 aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc
     9421 cacccgccgc cagggatccc accaaggttg acgccaaata acccggtcag aaaggtactg
     9481 ggtaaaaaga ccattgccat caacgacatt gtataggtac gacgagctaa attttcctgc
     9541 atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg
     9601 tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc
     9661 atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag
     9721 cgacgcatca caattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc
     9781 tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattca
     9841 ctggaatgat cggtcaacgc atcgcacaca tccaccagcc atcccccgca atcggtcgga
     9901 cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg
     9961 gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt
    10021 tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gaaacggctg
    10081 actcggggcc gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt
    10141 gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga
    10201 tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc
    10261 atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccactgtg
    10321 cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc
    10381 atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa
    10441 aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt
    10501 atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa
    10561 cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg
    10621 gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat
    10681 gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga
    10741 actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt
    10801 tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga
    10861 aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc
    10921 cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac
    10981 taactctgca ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg tcgatgctaa
    11041 cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga aacataccta
    11101 ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc
    11161 cggcggtcat aaccctttga attttgttca taaactggcg gatggatcta ctcgtcatgt
    11221 gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca
    11281 tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc
    11341 gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag gccaaatgca
    11401 gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca
    11461 cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgccc
    11521 cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt
    11581 cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgcgt
    11641 aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc
    11701 gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta
    11761 tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg
    11821 agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt
    11881 acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc
    11941 ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg
    12001 tgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac catccgccag
    12061 ggcttgttga atgaaactga acaccatttt gcggcactct tccaccggct ggcgcaaaag
    12121 attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgcta
    12181 caacccttcc tgccgaaact ccagcggctg acttagcggg atgatgtcga gaaatccggt
    12241 agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt
    12301 tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca tggcgtcaag
    12361 aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg caccttcaca cgtcgtgatt
    12421 cagacgggtt aattgttcat acttactgca tcgttccctt cacagattga aacaatactc
    12481 tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt atactagcct gttagcagta
    12541 tcgaacagtt tagcccgagt ttaattcagc acaggctccg atcaggatga tttattcata
    12601 gcaggaacca acaaaaatgc gataaataac agaccactga ttagcagaaa ggtaccgaaa
    12661 atgccatagt ttgcgcctcc ctcagcatca attgactcaa taatttggtt gaagccagta
    12721 tttccgatgt ctggtcactt ctactgcgat taaaaaataa agcacttctg gaaatgaagt
    12781 cattcaccgt atggggaaca gaaaccattt tttcacccat tctcgaaaaa tgaaaattag
    12841 ttcgattatt gagaatagag ccacacaggg caataccaat cgcgccccca agattacgtg
    12901 ttaaattaaa tacaccagag gctaacttca gccgttcttt tggaattccc cccagcgtta
    12961 atgttacgat gggggccatc gcaaactgct gcgaaatccc tctaattgcc tgtggaaaca
    13021 gtaattcctg ccatccccat tcgtgggtta taggggtaaa aagatacatt gaaaacacaa
    13081 atccgcccag ccccgccatc aacagccatc gcagatttat ttttttactt aaccagaagt
    13141 aaaagggaac agaaaataac tggaaaatac ctgttgtgca aacggcaaaa cctatctctt
    13201 cggcatttaa tccgcgaatc tgccccaaaa acacaggtat taaatataca gtagagaata
    13261 tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt gaaattttta tccttaaaag
    13321 catgaagatc cattatcgga ttggatattg taagtgtacg cgcagcaaat aaaataaaac
    13381 tcaccaatgc cagaactgat gtcagcaaaa tagtattatc atccagccat ccccagcgcg
    13441 ctccttcttc aagggtatat tccagacatc ccagagtcat ggccaacagg ataatactcg
    13501 gataatcagc aactttcagt aaagagagat caggtttatc aaaattaacc agaaaaggaa
    13561 tactcaaaac taaatatatg ccaggtaata tattgatata aaatagccat cgccagtcga
    13621 ggttatcagt gagccagccc cccagagttg gccctagcgt cggtgacagt gaggccagcg
    13681 cactgactac cgcagcagcc aaccccaact ctttaccctg gtaataaata aacgccatcg
    13741 tgaaaactaa agggatcatg gatgcgccag cagctccctg taaagcacga aacaaaatca
    13801 tgatctgaat attccatgca agtccacacg cgatactcat taaagtaaat attcccgcgg
    13861 atagagtaaa aagccaacga gtggaaaata cccgtgacag ccacccagag agcggaataa
    13921 caataatctc agcaattaaa tagctggtct gaagccaggc catttcgtct ttacctgcgg
    13981 acagaccacc acctatttca tttagggatg acgatacaat ttgaatatca agctgagcaa
    14041 taaataatcc catacacata ctggcgaaag caatatgttt acgcatagac atcatctctc
    14101 atcaacactg acggtgacgg acagtcccgg gcgaagaagt tgctttaatt cctttgcgtc
    14161 gtcaaatgca atccgtaccg ggacacgctg aacaattttg gtgaaattgc cagtggcatt
    14221 ttctatcgga atcaagctga aactggctcc tgtcgctggg gaaaggcttt caatgtgacc
    14281 gtgaaacact tcccctttga gaatatcagc acggatctca gccttcattc ctggcttcat
    14341 tcccgctatt tggttttctt tataatttgc atcaacccac aactcactga ctggtactag
    14401 tgaaactaac gatgtgccgc cttccaccca actgcctgta tgggcgctgc ggtttgcgac
    14461 aatgccatca atcggcgca
//
LOCUS       DADLTY010000106        14369 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.106, whole genome shotgun sequence.
ACCESSION   DADLTY010000106 DADLTY010000000
VERSION     DADLTY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.106"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..265)
                     /locus_tag="JN334_002723"
     CDS             complement(<1..265)
                     /locus_tag="JN334_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY7438815.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNV"
     gene            complement(362..3463)
                     /locus_tag="JN334_002724"
     CDS             complement(362..3463)
                     /locus_tag="JN334_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HAY7438816.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            3761..4954
                     /locus_tag="JN334_002725"
     CDS             3761..4954
                     /locus_tag="JN334_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY7438817.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(5152..6405)
                     /locus_tag="JN334_002726"
     CDS             complement(5152..6405)
                     /locus_tag="JN334_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAY7438818.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6732..7922
                     /gene="hmpA"
                     /locus_tag="JN334_002727"
     CDS             6732..7922
                     /gene="hmpA"
                     /locus_tag="JN334_002727"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HAY7438819.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(7967..8305)
                     /gene="glnB"
                     /locus_tag="JN334_002728"
     CDS             complement(7967..8305)
                     /gene="glnB"
                     /locus_tag="JN334_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HAY7438820.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(8366..9700)
                     /gene="glrR"
                     /locus_tag="JN334_002729"
     CDS             complement(8366..9700)
                     /gene="glrR"
                     /locus_tag="JN334_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HAY7438821.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9690..10403)
                     /gene="qseG"
                     /locus_tag="JN334_002730"
     CDS             complement(9690..10403)
                     /gene="qseG"
                     /locus_tag="JN334_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HAY7438822.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10568..11995)
                     /gene="qseE"
                     /locus_tag="JN334_002731"
     CDS             complement(10568..11995)
                     /gene="qseE"
                     /locus_tag="JN334_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HAY7438823.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12553..>14369)
                     /gene="purL"
                     /locus_tag="JN334_002732"
                     /gene_synonym="purI"
     CDS             complement(12553..>14369)
                     /gene="purL"
                     /locus_tag="JN334_002732"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAY7438824.1"
                     /translation="GEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIK
                     LSANWMAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEERE
                     MTSPLSLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLG
                     DKPADVRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADI
                     ASLGDDRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVIT
                     ANGQTVFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDI
                     NEDVAAPYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLE
                     DFHALVACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMM
                     SNLRELIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEG
                     RVEVRDAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIM
                     MPHPERVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
BASE COUNT         3418 a         3893 c         3658 g         3400 t
ORIGIN      
        1 cgacattacg cgccacgagg gtatcaacaa aggtactttc tttcgaaata tccccctggg
       61 cgttagtttc aattaactgt acctgtactg aagaatcttt tgccgcatcc tgaacgccct
      121 gacgcactcc agcgtaatat ccctgggtat caaggtatat tgcaccaatg gtcatttctt
      181 tatccgcagc cctggcaaat agtgcgctac ctaatagcgt agccattaat aatagattac
      241 gggttgttct cattttttta ggcatagtgc ctcctgtagg gtttttatta acaacggctt
      301 attctaatta ttttgtgatg agcggcagcg cgtgccgctc aaatattaca gttaataggg
      361 tttagtgaac gtagttaaaa ataaaaggca tcacagtggt gaataatgcc gctttcggcc
      421 aggccggatt aatcgccgtc aattcagcgc gagcagactc atattgcgca acctccccga
      481 gtcccgcgga cgccagcatc gccaccatca ggcatttttc tttatgctgc tgttgtaaat
      541 cgccatacag cgataacagg tcaggttgtg aaacagcaaa gaaatccgcc tcaatactgg
      601 ttttcgccat ctcttgcgcc cactgtttca tttcactaaa cagttgctgt gcggtttgct
      661 gttcacccag cagtcgcagc gccattcctt gccagaagag ataatcaacc ggctgatcgt
      721 tgtaataact gtggatgtta atggtgcgat cgccggtcgc cgccagacgt aaacaacgca
      781 tcgcttcagt ttcatcgccc tgcgcgttgg cgcatatcgc ctgccagaac cagatgtcgt
      841 tatcagtttg ccccggtaaa cggccttcgc ttaaattctc cggataatac agcgcggcat
      901 gaagcagttc gctggcctgc tgcggctctc tggcatcaag atgctgccag gcgcgtaata
      961 actggttgag gataaactga ctggtgacct tcccttcccc gccttcccac gggtggaatt
     1021 tacgcgtggc gagaatgtcc gccgctttgt ctgcctgacc cgtgagatgc cacaaattga
     1081 gcagttctgc ggtcatgtcg tcgcgtttca gcgcaatttc cagattattt tccagacgcg
     1141 ccagtcgttt ctccggtgtg gctccactta acttatccag caaatcccgt tcgaaaagca
     1201 gacgtgcatc ctgcggcgca agctgataag cattatcaag ataacgcgcg gccagctcat
     1261 aatcgtgttt cttattccac gcatggatcg ctaacccgcg ccagccgtcg gcaaactccg
     1321 gcgacatctc tacgcaacgt tgccataagg caatggcttt gttgtagcta cgtttgttgt
     1381 agtagaagca ggccagtaaa tggcgagcaa accaacactc ttcaatactc tccagcgccg
     1441 ccacttcttc cagcgtattc gggaaacgga caaactgcgg gaagacatca atggcttttg
     1501 cgaccagttc gccacgttcg gctttcggca gcaagctggc ttgcaggtaa agcggcagcg
     1561 tgcgctggca gtccagtgcg ttcagcatct ctgccgccag ggtgggcatt ccccagttaa
     1621 tcagttgccc ggcggtcatc agagcgttaa cgtcgcgtcc ctgacacaga ccgcgccact
     1681 gcgcgagggc tgattcgcta cgaccatcga accagttcag ccaccacaga gtggcgttca
     1741 gcggataatc acgcagcagt ttctcgcgct gcaaacgcgc gttgtcctga cgaccactta
     1801 acaccagcag cagattatgc aagcaaagca cttcctgatt ggttgggcag gcacgaagac
     1861 tttgttggca aaaatccaga cccgcgtcga agttatcatt acgcgccgcc agtcgtgcca
     1921 gaccataata gccgcctgct ttactattgc cgctccagac cgcacgccag aaatcctctt
     1981 cggcttgttg atattgtccc tgacgttcgt aagcactggc gcgaatcaaa cttgcttgtc
     2041 cacactgcgg ttttttgttc agcgcatgtg cacgtttcag cgcctgactg gcatacgcca
     2101 ccgcttgcgg gaaatctgcg cggttatatt ccagcatcgc cagcgccagg ttacagcgat
     2161 aatccagcgg gtccagcgcc acgccgcgca ggtagtaatc gaacggtgaa cggctggcgt
     2221 gatgatattg ctccagatgc tgaccgataa accaggcttc atctgtactg gtaatgtctt
     2281 gtgctgccag tggcgctttg gcgacgtccg gcaacggcaa ctcctgcggc tgatgttcct
     2341 gataactcag tacaatattg ccgtcggcat cggagagttc aatggtcagt ctgtcaggat
     2401 tgataccgtg caacacgccc tggatggcgg tcgctggtgt cagggccacg gcatcatcga
     2461 gcaacgcgtt gcatttgccg atttcgcgga tcgccagacg gtatccgttc aacggagaga
     2521 tggcatacag cccccactca atcccccgct cactacgctg gagttttatc accgcatcgc
     2581 gggaggcatt ttgcaccatg cccagagaat gataaggcag gaaatactgc tcgaaacgct
     2641 tctcttcgta agcatcaagc caggtaaaat caggctggtt atcggcaaaa ataccggtca
     2701 tcagttcgat atacgggccg ttattgtcgg tcagactctt atcccacgcc tggccaaatt
     2761 cactgtgtcc ccaactccac tgttttttac ctggcgcaat atggtggttg gcaacgtgca
     2821 gcaaaccgcc atcttcatcg tgacaccacg cgccgacaaa atcgtactgc gatttttcgg
     2881 ccatatatga ggttggaaca ggcacatttt tatagcgaga aatgtccact ccggcggagt
     2941 agtccacttt gtagtaagtg ccggtggcga tggggaaagc ggagacggcc cgtttgccgt
     3001 gatcaaacac cgcagtcaca tccggtggga aaacgctctg atgcccttca ccccctttca
     3061 ctgccgggtt gacccaccac aagaaatgac gcggcgtggc gttgccgtta tagacgcggc
     3121 tggcgatttc cagtgccgcc cggtcagggc gcagggtgaa acctgtcatc acctgtaaac
     3181 catgcatcgg ctccgtttcg ccgacccaca ccgtctgtgc accgtcttca tgggcttcga
     3241 gggtgaaatc aacgggcata aaggtggtcg ggcgatggtg ttgcggccag ttaaactcaa
     3301 tcccgccgga gatccacggt cccagcatcc ccaccagcgc aggtttaatg acttcattgt
     3361 gatagacaaa atcgcgctgt ttcactttat cccatgcgcg atgcacccga ccgcccagtt
     3421 ccggcaggat catcactttg atgtagtcgt tttccagcca caccgcctgc taggatttca
     3481 gggttttctg ctcgctcagc gtatcggtca cgccgtaggg ataaaccgcg ccggacgatc
     3541 cctgataaac gcgattttcc aggaacatgg gatgtatatc ctgcggcccg gtttcatagg
     3601 tcgggatctc aacgcgctct tgccacactt ttactggagt catggtgccc tcaatattaa
     3661 caagacatac tgaattaaaa gatttgtggc agtgtattga acaatctggc aatgttttcg
     3721 cggaataatc acgcaattaa ctaaacaagg tttagtgaag atgagagcct gcattaacaa
     3781 tcaacagatt cgccaccata acaaatgcgt gattctggaa ctgctgtacc ggcaaaagcg
     3841 cgccaataaa tcaacgctgg cccggctggc gcaaatttcg attccggcag tcagtaatat
     3901 tttgcaggaa ctggaaagcg aaaaacgggt ggtgaatatt gacgatgaaa gccagacgcg
     3961 cgggcatagt agcggtacat ggctgattgc gccggaaggt gactggacgc tgtgcctgaa
     4021 cgtgacgccc accagtattg agtgtcaggt tgctaatgct tgtttaagtc cgaaaggcga
     4081 atttgagtat ttacagattg atgtaccgac gccgcaggcg ctgctgtccg aaatcgaaaa
     4141 atgctggcat cgccaccgta aattgtggcc ggaccgcacc atcaatctgg cgctggcaat
     4201 ccacgggcag gttgatccgg tgaccggcgt atcgcaaacc atgccgcaag cgccgtggac
     4261 aacgccagtt gaggtgaagt atctgctgga agagaagctg ggcattcggg tgctggtcga
     4321 taatgactgc gtgatgctgg cgctggcgga gaaatggcaa aataattcgc aggaacggga
     4381 tttctgcgtg atcaacgttg attacggcat tggctcgtcg ttcgtgatta acgagcaaat
     4441 ttatcgcggc agtttgtatg gtagcggaca gattggtcac accatcgtta atccggatgg
     4501 agtcgtctgc gactgtggac gttacggcag cctggaaacc gtcgcctcgt taagtgcatt
     4561 aaaaaaacag gcgcgggtat ggctaaaatc acaaccggtt aatactcagc ttgagcctga
     4621 aaaactgaca accgcgcagt taatcgctgc ctggcagagt ggagaaccgt ggatcaccag
     4681 ctgggttgat cgctctgcca atgccattgg tttgagtctg tataacttcc tcaacatcct
     4741 caatattaat cagatttggt tgtacggtcg cagttgtgcc tttggtgaga actggcttaa
     4801 tactattatt cgccagacag gatttaaccc gttcgaccgc gacgaaggac cgagcgtgaa
     4861 agcgacgcaa attggcttcg ggcaattaag ccgcgcacaa caggtgctgg gaattggcta
     4921 tttgtatgtt gaggcgcagt tacgacagat ttgatggcgc gataacgtag aaaggcttcc
     4981 cgaaggaagc cttgatgatc ataaacgaaa aattgcctga tgcgctacgc ttatcaggcc
     5041 tacacggaga ttgcaatata ttgaatttgc aagattttgt aggcctgaga aggtgttcat
     5101 gccgcatcca acatgaacaa cgagcacatt gtcagcaaat caccgtttcg cttatgcgta
     5161 aaccgggtaa cgtgcgcaga tgtcgagaac tttacctttg atgcgctcga taacggcttc
     5221 atcattgatg ctgtccagca cgtcacacat ccagccagcc agttctttcg cttcggcttc
     5281 tttaaagccg cgacgggtaa tcgccggagt acctacacga ataccggagg tcacaaacgg
     5341 gctcttcgga tcgttcggta cgctgttttt gttgacggtg atgttagcac ggcccagagc
     5401 ggcgtctgct tctttaccgg tcaggttttt atcaaccaga tcaaccagga acaggtggtt
     5461 atcagtgccg ccggaaacca ctttgtagcc gcgctcgagg aacacttcta ccatcgcttt
     5521 agcgtttttc gcgacctgct gctggtaagt tttgaactca ggctccatcg cttctttcag
     5581 agcaaccgct ttaccggcga ttacgtgcat caacggaccg ccctgaccac cagggaaaac
     5641 ggcagagttc agttttttgt acagctcttc gctaccacct ttcgccaaga tcaggccgcc
     5701 gcgcggaccc gccagggttt tgtgagtggt ggtagtaaca acgtgagcat gaggaaccgg
     5761 gttcgggtag acgccagcag caaccaggcc cgcaacgtgc gccatatcaa cgaacagata
     5821 agcaccgatg ctgtcagcga tttcacgcat tttcgcccag tcaaccacgc cggaatatgc
     5881 agagaaacca ccgataatca ttttcggctt gtgttctttg gcttgttttt ccagatcggc
     5941 gtaatcgata tgaccggtag catcgatacc gtaaggaacg atgttgtaca gtttaccgga
     6001 gaagttaacc ggagaaccgt gagtcaggtg accgccatgc gccaggttca tacccagaac
     6061 ggtatcacct ggttccagca gcgcggtgta gaccgcaaag ttagcctggg agccggagtg
     6121 cggctggacg ttagcgtagt cagcgccgaa cagttctttc gcacgatcga tcgccagttg
     6181 ttcaacgata tcaacatact cgcaaccgcc gtagtagcgt ttgcccggat aaccttcagc
     6241 atatttgttg gtcagctgag aaccctgcgc ctgcattacg cgcgggctgg tgtagttttc
     6301 ggaggcgatc agttcgatgt gctcttcctg acgtactttt tcctgctcca tagcctgcca
     6361 cagttcggca tcataatcgg caatgttcat ttcacgcttt aacatccgca tctcctgact
     6421 cagctaacaa taaaattttt ggcctttata ggcggtcctg ttggacaacg gcgaacagta
     6481 taaccgaatc attgtgcgat aacaggtctt gacaaaggaa tttacgcaaa cgattacctt
     6541 caggctacgc aaggctttgg agaataaaga gcttgcaacc ggaaacggat ttcttttcag
     6601 gtttgtgatg caaatttttc acttcatcac attctttctg aaaaacacca aagaaccatt
     6661 tacattgcag ggctattttt tataagatgc atttgagata catcaattaa gatgcaaaaa
     6721 aggaagacca tatgcttgac gctcaaacca tcgctacagt aaaagccacc atccctttac
     6781 tggtggaaac ggggccaaag ttaaccgccc atttctacga ccgtatgttt actcataacc
     6841 cagaactcaa agaaattttt aacatgagta accagcgtaa tggcgatcaa cgtgaagccc
     6901 tgtttaacgc tattgccgcc tacgccagta atattgaaaa cctgcctgcg ctgctgccag
     6961 cggtagaaaa aatcgcgcag aagcacacca gcttccagat caaaccggaa cagtacaaca
     7021 tcgtcggtga acacctgttg gcaacgctgg acgaaatgtt cagcccgggg caggaagtgc
     7081 tggacgcgtg gggtaaagcc tatggtgtac tggctaatgt atttatcaat cgcgaggcgg
     7141 aaatctataa cgaaaacgcc agcaaagccg gtggttggga aggtactcgc gatttccgca
     7201 ttgtggctaa aacaccgcgc agcgcgctta tcaccagctt cgaactggag ccggtcgacg
     7261 gtggcgcagt ggcagaatac cgtccggggc aatatctcgg cgtctggctg aagccggaag
     7321 gtttcccgca tcaggaaatt cgtcagtact ctttgactcg caaaccggat ggcaaaggct
     7381 atcgtatcgc ggtgaaacgc gaagagggtg ggcaggtatc caactggttg cacaatcacg
     7441 ccaatgttgg cgatgtcgtg aaactggtcg ctccggcagg tgatttcttt atggctgtcg
     7501 cagatgacac accagtgacg ttaatctcta ccggtgttgg tcaaacgcca atgctggcaa
     7561 tgctcgacac gctggcaaaa gcaggccaca cagcacaagt gaactggttc catgcggcag
     7621 aaaatggcga agttcacgcc tttgctgatg aagttaagga actggggcag tcactgccgc
     7681 gctttaccgc gcacacctgg tatcgtcagc cgagcgaagc cgatcgcgct aaaggtcagt
     7741 ttgatagcga aggtctgatg gatttgagca aactggaagg tgcgttcagc gatccgacaa
     7801 tgcagttcta tctctgcggc ccggttggct tcatgcagtt taccgcgaaa cagttagtgg
     7861 atctgggcgt gaagcaggaa aacattcatt acgaatgctt tggcccgcat aaggtgctgt
     7921 aatttgatgt tgccggatgg aaacatccgg caacccttga cgcggtttaa attgccgcgt
     7981 cgtcctcttc accggtacgg atgcgaatga cccgagccac gtcaaagacg aagattttac
     8041 cgtcaccgat tttgccggtt tgcgccgtgc gaataatggt atcgacacag gtatcgacaa
     8101 tgtcgtccgg tacgacaatc tcaattttca ctttcggcag aaaatccacc atatactccg
     8161 cgccgcggta cagctcggta tggcctttct ggcgaccaaa gcctttcact tcggtcaccg
     8221 tcatgccggt aataccgact tcggccagtg cttcgcggac atcgtccagc ttgaagggtt
     8281 ttataatcgc atcaatcttt ttcatgctat tccttgaaaa ggttgcctgt cttttgatct
     8341 gctaaacgta acacataacg ccaattcatt ccttgaaatc gtttgcatcc agctcgtgtc
     8401 gggaaagcag tttataaaat tctgtccggt tgcgccccgc cattctcgcc gcgtgggtga
     8461 cgttgccttt ggtgatttgc agcagcttac gcaagtagtt gagttcaaaa tgattacgtg
     8521 cctcaacaaa ggttggcagc gccgtatttt caccctccag cgcctgctcc accagcgcat
     8581 cactaatcac cggagatgag gtcagcgcca cgcactgttc aatcacgttg accaactggc
     8641 gcacattacc cggccagctc gcggtcatca ggcgtttcat cgcatcggta gagaacgcgc
     8701 ggacaaacgg tttatgtcgc tctgccgcct ggcgcaacag gtgatttgcc agtagcggaa
     8761 tgtcttctgt gcgctccgcc agtgccggaa ttttcaggct gacaacgttg aggcggtaat
     8821 agagatcttc gcggaattcc ccgcgcgcca tcgcttttga cagatcacgg tgagtggcag
     8881 aaataatccg cacattgata tcaatatcgc ggttactgcc cagcgggcgc actttacgct
     8941 cctgcaacac gcgcagcagt ttgacctgta acggcgcagg catatcgcca atttcatcga
     9001 gaaatagcgt tccgccttcc gccgcctgga ataaaccttc gcgattgctg acagcgccag
     9061 taaacgcgcc acgcgcatga ccaaacagct ccgactccag caattgctcg ggcaatgcgc
     9121 cacagttaat agcaataaat ggtttgctgt tgcgcgggct ggcgttgtgg atagcctggg
     9181 cgaaaatctc tttcccggtg ccgctctgac cgttaatcaa aacgctgacg tctgattgcg
     9241 ccaccagccg cgcctgttcc agcaaacgca gcatcagcgg gctgcgggtg acaattgcct
     9301 cgcgccagcg ttcatcggtg gctggcgcgg attgctccag cgcatcgtca attgcctgat
     9361 atagcgcatc tttatcgaca ggcttggtga ggaaactaaa aacgccctgc tgtgttgcag
     9421 caacggcatc ggggatagaa ccatgcgcag taagaataat tactggcatt cccggctgca
     9481 ctttctggat ttcagcaaat agctgcatac cgtccatttc atccatccgc aggtcgctga
     9541 taactaaatc tactttttcg cgattcagta cccgtaatcc ttcagcgcca ctttccgccg
     9601 tgaccacact gtagccttcg ctggtcaggc gcaggccaag cagtttcagc aatcccggat
     9661 cgtcatcgac caataataaa tgcgcaggtt tatggctcat caggagtgac ctcatgggtg
     9721 gatggcgcgg gcttttcact ctcgtgcggc gtatccggcg agaaatttcc agccggtttg
     9781 cgggtcgaga gctggcgttc aatatcggtc aggttttcca gcttgcgggt ggtgagttcc
     9841 agttgctgct gtagaacgtg atgttgctgg cgcaatgtat ccagctcgct gtcgctggac
     9901 tgctggagtt tactgtaacg ttggcgctct tccgccagtt gcagttgcag cgcctgaccg
     9961 tcgcgccaga gttgatacag tgggcgaacc tgtgccggga tctcggtact taacgcttca
    10021 atacgcgcga ccagctggcg gcgctcatac ggcgtaattt tggcgtcggc gagcaaaatc
    10081 ccttgtttaa aggtattttg ccagctgccg tcgtcatatt ggcgagcttg ctgacgcgac
    10141 tgcgcaggca ttaaacgatc agcacaatcc atcgcccgca gccagtaaag cggattggtt
    10201 tcggttgatt taccttgcag cgcccagatg tcgctacatt cagtagaaag atagtcagcc
    10261 agttgataaa ccggaatttt ttcttctgct ggcgtatcaa tggctggctt attgtgattc
    10321 tgcacgcaac ccagcaatgc cagacatggc agccctgcca gccacagccg tcggggcaat
    10381 aatcgttgaa aaatgtgtcg catattcacc agacttaaag cctatcccag tgggcgtaat
    10441 tgttgcagac agtctggaca tggacagcgc ggagaaaccg gggcgtacat atcgtacgtg
    10501 aagatttcga gcactacccg gggccgaact gacaaataaa atagcctgat aggataggct
    10561 gttagcatta tttcgtgttt ttcgacgacg gtaattcaat gcggaaacaa acgtcttgcc
    10621 cgctctcgtc gaccagatac agttcccctt gcatacggcg aatacaatcc ctggcaatgc
    10681 ttaatcccag accgctgccc ttcaccgccc cttttcgctg gtggcttccc tgaaaaaagg
    10741 gttcgaagat catggcgcgt tcctcttgcg gaatgggcgt gcctgtattg atgacatcaa
    10801 tataaacccg cgcaccatgt aaactgctgc gaaggcaaat gttaccggat tcagccccgt
    10861 agtgcaccgc attggagtaa agattatcca gtacgctcat cagcagcatt ggctccgcca
    10921 ggcaagctgt tgctttgaga tcgacgtcgg tatgcatcat tttagcccgt gcgggcaggc
    10981 tatgagcaga aaccactgtc tccaccagcg gtgctaattc aacattctcc agttccaccg
    11041 cactgtccgc ctgtttacgg ttgtaatcaa gcagttgttc gatcagtttt tgcaaattgc
    11101 agctgctgct atcaagaatg ctcaccacct ctttttgctc tggcgtaagc ggcccgacaa
    11161 cctgatcagc cagtaattca gtgccctcgc gcatactcgc cagtggcgtt tttaattcat
    11221 gagataaatg tcttaaaaat tgatggcgtt gggattccag ccatgacagg cgctcactta
    11281 accaaagaat acgttgccca accgagcgta actcgctcgg tccacagaac gaaacgctat
    11341 tgcccagaga acgcccttcc cccagacggt tgatcatgcg ctcgatattt ttcaccggcc
    11401 cgataatcat ccgcgtgaaa agtagcacca tcaccagact caccagaaat agcaccagcg
    11461 attgccaacc aaaatattgc ccacgttcgg cgatttcacg ctgaagttgc tgcccacgag
    11521 agaacaccac tgtgcgcgtg gcctgcacca tttcggtatt ggcactggca aaggcttcaa
    11581 gacgcgcggc ggcggcagca tcgggaccac tgttgttaca ctgaagttgg gccagattgt
    11641 tcaaatcctg acgcaatgcc tggtaaagtt tatcgtctgg cagtacgcct gcgtgggcat
    11701 cgagcatttc gctgtaacgc ttgcgctggc tttgataaac cttcgcaagc gtagggtcgt
    11761 ccagcacgca atactgacgg tagctacgct ccatctccag cgccgcgttg gtcatcgctt
    11821 cactacgccg ggcatcaata agcgtagtgc ggttaaccag cgccgcctga tcgctaagcg
    11881 cattcaggct ttgccaggct tgccatgcca gcaccaacag gggtagtaga attagcaaaa
    11941 atgccagcat taccagttgt cgtaatgagc ggggaaaaac gggccagcgt ttcaaggtgt
    12001 tactctcgtc agacgcgaat agcctgatgc taaccgaggg gaagttcaga tacaacaaag
    12061 ccgggaatta cccggctttg ttatggaata aggcggtgcc taactcgacg tttcgcccga
    12121 tggttgatat agctacgctg atatcagaag ttggacggca ggcaccttgt tgtgcgtcat
    12181 tcgaatttta tgtagcacgt cccgaagggg ctgacataag tcggtgaatg agccactgct
    12241 tactattatg cagtaactgt gccaataaag aaaatagttt ggtaacgtac tgattatacg
    12301 ttgctttgag ggatttatgg cttcttcgct atttgaatgt aaatcagcca ctgtgtcgct
    12361 aaaaagagac aacttaagat aaacttatta gattacaata aaatcaatca tttaagtgtc
    12421 gccagaaagc gacacggtaa accacccatt gtcgtgattt acagacacaa aaaagctccc
    12481 ggagttggga gcttatgata gtggttggtg cttaatgccg catccgggct gcaaaaccaa
    12541 tgaactgatg acttacccca actgcttacg cgcattgcgg aaaatgcgca tccatgggcc
    12601 atcctcgccc cagttttccg gatgccagga gttgctgaca gtacggaaaa cacgttccgg
    12661 gtgcggcatc ataatggtga ctcgaccact ttcagtcgta accgccgtaa taccgttcgg
    12721 tgaaccgttc gggttagccg ggtaggtttc agtgactttg ccgaagttat cgacatagcg
    12781 cagtgccacc agccctttgc tttccagtgc cgccagatgc gccgcatcac gcacttccac
    12841 gcgcccttca ccatgagaga cagcaatcgg catttgcgag cccaccatcc cctgcaacag
    12901 cagagacggg ctttgggtta cttcaaccag gctgaaacgc gcttcaaagc gatcggaggt
    12961 gttacgcaca aaacgtggcc acaactcact ccccgggatc agttcacgca ggttagacat
    13021 catctggcaa ccgttacata cccccagggc cagcgtttgc ggacgatgga agaaggttgc
    13081 aaactcatcg cgtacacggt cattgaacag gatagacttc gcccaacctt caccggcacc
    13141 cagcacatca ccgtaggaga aacctccgca cgcgaccagg gcgtgaaaat cctccagtcc
    13201 cgtacgtcca gtcagcaggt cgctcatatg cacgtcgata gcatcaaagc ccccacgttg
    13261 gaaagctgcc gccatttcaa catgcgagtt cacgccctgc tcacgcagta cggcaacttt
    13321 cggacgtgcg ccagtggcaa tatacggtgc tgccacatct tcgttgatat cgaacgacag
    13381 ttttacattc aggcccggat cggcgtcgtt agatttcgcc tgatgctcct gatcggcaca
    13441 ctccgggttg tcacgcaggc gctgcatctg ccaggtagtt tctgcccacc agacacgcaa
    13501 cgtagtgcgg ctttcgctga atacagtctg cccgttggcg gtaatcacaa aacggtcacc
    13561 ggaaaccgcc tgcccaacat aatggacgca atccgcaagc ccatgctgtg ccagtacgga
    13621 ctcgaccgct tcacggtcag cggcacgaac ctgaatcacc gcacccagtt cttcgttaaa
    13681 caacgccgcc aggcgatcgt cacccagaga ggcgatatcc gcgtcaatgc cacaatgacc
    13741 tgcaaaggcc atttccgcca gcgttaccag caggcctcca tcagagcggt cgtgatacgc
    13801 cagcagctta cgctgtgcaa ccagcgcctg aatcgcgtca tagaagcctt tcagttggac
    13861 aacatcgcgt acatctgccg gtttgtcgcc aagctgacga taaacctgcg ccagcgccgt
    13921 tgcgcccagc gcgttattgc ctttgcccaa atcaatcagc agcagtgcgt tatcttcggt
    13981 acaaagctgc ggcgtgatgg tgtgacgtac atcttccacg cgggcaaatg cagaaatcac
    14041 cagcgacagc ggcgacgtca tttcgcgctc ttcgttacct tcctgccagc gggttttcat
    14101 cgacatagag tctttaccca ccgggatcgt caggcccagc gccggacaaa gttcttcacc
    14161 cacggcttta acggcttcat acaggcccgc atcttcacca gggtggcctg ccgccgccat
    14221 ccagttggcg gaaagtttaa tgcgtttgat atcgccaatc tgtgttgcgg caatattggt
    14281 taacgcttca ccgaccgcca gacgggcaga ggcggcgaaa tccagcagcg caaccggcgc
    14341 acgctcgcca atcgccatcg cttcaccgt
//
LOCUS       DADLTY010000107        13964 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.107, whole genome shotgun sequence.
ACCESSION   DADLTY010000107 DADLTY010000000
VERSION     DADLTY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.107"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="JN334_002733"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="JN334_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7438825.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="JN334_002734"
     CDS             2219..2767
                     /locus_tag="JN334_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HAY7438826.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="JN334_002735"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="JN334_002735"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HAY7438827.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /locus_tag="JN334_002736"
     CDS             complement(3664..4854)
                     /locus_tag="JN334_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HAY7438828.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JN334_002737"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JN334_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HAY7438829.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="JN334_002738"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="JN334_002738"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAY7438830.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="JN334_002739"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="JN334_002739"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAY7438831.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="JN334_002740"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="JN334_002740"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAY7438832.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="JN334_002741"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="JN334_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY7438833.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="JN334_002742"
     CDS             complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="JN334_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HAY7438834.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3551 a         3563 c         3526 g         3324 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagagta cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcctggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatggt ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgct
//
LOCUS       DADLTY010000108        13941 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.108, whole genome shotgun sequence.
ACCESSION   DADLTY010000108 DADLTY010000000
VERSION     DADLTY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.108"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="JN334_002743"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="JN334_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HAY7438835.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="JN334_002744"
     CDS             complement(2138..2584)
                     /locus_tag="JN334_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAY7438836.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="JN334_002745"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="JN334_002745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HAY7438837.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="JN334_002746"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="JN334_002746"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAY7438838.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="JN334_002747"
     CDS             complement(5347..6009)
                     /locus_tag="JN334_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HAY7438839.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="JN334_002748"
     CDS             6182..6595
                     /locus_tag="JN334_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAY7438840.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="JN334_002749"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="JN334_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAY7438841.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="JN334_002750"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="JN334_002750"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HAY7438842.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="JN334_002751"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="JN334_002751"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAY7438843.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="JN334_002752"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="JN334_002752"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HAY7438844.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="JN334_002753"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="JN334_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HAY7438845.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="JN334_002754"
     tRNA            complement(9861..9948)
                     /locus_tag="JN334_002754"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="JN334_002755"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="JN334_002755"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HAY7438846.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="JN334_002756"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="JN334_002756"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HAY7438847.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..>13941
                     /gene="hyaC"
                     /locus_tag="JN334_002757"
     CDS             13301..>13941
                     /gene="hyaC"
                     /locus_tag="JN334_002757"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HAY7438848.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTV"
BASE COUNT         3503 a         3547 c         3688 g         3203 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga t
//
LOCUS       DADLTY010000109        13816 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.109, whole genome shotgun sequence.
ACCESSION   DADLTY010000109 DADLTY010000000
VERSION     DADLTY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.109"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..771)
                     /gene="osmF"
                     /locus_tag="JN334_002758"
     CDS             complement(<1..771)
                     /gene="osmF"
                     /locus_tag="JN334_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HAY7438849.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEY"
     gene            complement(1023..3320)
                     /gene="bglX"
                     /locus_tag="JN334_002759"
     CDS             complement(1023..3320)
                     /gene="bglX"
                     /locus_tag="JN334_002759"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HAY7438850.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            3516..5231
                     /gene="dld"
                     /locus_tag="JN334_002760"
     CDS             3516..5231
                     /gene="dld"
                     /locus_tag="JN334_002760"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY7438851.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILIQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(5269..6201)
                     /gene="pbpG"
                     /locus_tag="JN334_002761"
     CDS             complement(5269..6201)
                     /gene="pbpG"
                     /locus_tag="JN334_002761"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAY7438852.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(6375..6962)
                     /locus_tag="JN334_002762"
     CDS             complement(6375..6962)
                     /locus_tag="JN334_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HAY7438853.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            7132..7710
                     /locus_tag="JN334_002763"
     CDS             7132..7710
                     /locus_tag="JN334_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7438854.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(7827..8588)
                     /locus_tag="JN334_002764"
                     /pseudo
     CDS             complement(7827..8588)
                     /locus_tag="JN334_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(8641..10167)
                     /gene="mdtQ"
                     /locus_tag="JN334_002765"
                     /pseudo
     CDS             complement(8641..10167)
                     /gene="mdtQ"
                     /locus_tag="JN334_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            10301..10384
                     /gene="yohP"
                     /locus_tag="JN334_002766"
     CDS             10301..10384
                     /gene="yohP"
                     /locus_tag="JN334_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HAY7438855.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(10755..11702)
                     /gene="dusC"
                     /locus_tag="JN334_002767"
     CDS             complement(10755..11702)
                     /gene="dusC"
                     /locus_tag="JN334_002767"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HAY7438856.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            11941..12339
                     /locus_tag="JN334_002768"
     CDS             11941..12339
                     /locus_tag="JN334_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAY7438857.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            12336..13031
                     /locus_tag="JN334_002769"
     CDS             12336..13031
                     /locus_tag="JN334_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HAY7438858.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            13161..>13816
                     /gene="cdd"
                     /locus_tag="JN334_002770"
     CDS             13161..>13816
                     /gene="cdd"
                     /locus_tag="JN334_002770"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAY7438859.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     "
BASE COUNT         3183 a         3729 c         3501 g         3403 t
ORIGIN      
        1 atactccttc aacaccgatt cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc
       61 ttgcggatcg cttaaggttt gcagccccag cgccgcgacc ggaccgtcag tgccgtaagc
      121 cattgcggca ttaacgccag aggtttgctg ggcagcggct ttaatcgtca ccgccgtgtc
      181 gccaccagcc agtgacagca actgatcctg accgagctta aagccatagg ctttttcaaa
      241 cgtgggtaac gcatcggcgc gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc
      301 gccctcttgc agataacgac tcaggtcggc aagcgaagtg agtttgtttt tctctgccac
      361 atcctgacgc acggcgatag tccaggtgtt atttgcaggc gcgggcgtca gccagattaa
      421 cttgttgtgc tccgcatcga gctttttgac tttctcgtag ccctgcccgg catttttcca
      481 cgctgcatcg ttttcatctt taaagaagaa agcgccattg ccggtatatt ccggatagat
      541 atccagttca tcggaagtaa tcgccccgcg ccccacagga gtcgttccga gttgcacttt
      601 attcaccgtc ggtacgccgt ggctttcgag tacctgcaag atgatgttgc cgagcagcgc
      661 gccttcggta tcgatttttg aaccgacttt aacgggggaa gccgcctgta gcggcaggct
      721 cacggctgcc aacataacca gtgaacctgc ccagagcttt aagagtggca tgatgctttc
      781 ctcattcttt tactgttgtt ttcagcgaat taagagaaaa gcatagttga taatgacaga
      841 gttagcctga gcggaaggat tcagttgcag aatcagataa atacttaaga ggcatattcg
      901 gtccggcgtt tcctgccgga tgcggcgcga gcgccttatc cggcctacaa agggcgaaaa
      961 ttcagcaagt taagatatga acatcaggca aagggcgcaa acgtcgcgcc cttactaaag
     1021 cattacagca actcaaactc gcctttctta acgcgtgcgg aatcagtgcc gataaataca
     1081 ttgaacttgc caggctcggc gtcatatttc atctgttgat tccagaactt cagcgcctca
     1141 atatcgatcg ggaagctgac agtctgagtt tcgcccggtt tcagggtgat tttctcaaag
     1201 cctttcagct gtttcacagg gcgactcatg gaagccgtca catcctgcaa gtacatctgc
     1261 actaccgtgg ccccctcgcg cttaccggtg ttcgtcacct gcacgctggc cgtcactttg
     1321 ccgtcacgct tcatggtcgg cgcagaaagt ttcacatcag agacggtgaa agtggtatag
     1381 ctcagaccat agccgaacgg ataaagcgct ccgttagctt catcaaaata acgcgaagta
     1441 tatttgttcg gcttgtcggc attatacgga cgaccggtgt tcagatggct gtagtacacc
     1501 gggatctgcc cgacagaacg cgggaaggaa attggcagct tgccggacgg gttgtaatcg
     1561 ccaaacaata catcggcaat tgcattaccg ccttcagtcc ccgcaaacca ggtttccaga
     1621 atcgcatcag cctgctgatc ttctttcacc agcgccagcg gacgcccgtt catcagcacc
     1681 agcaccagcg gtttaccggt ggctttcagc gccgcaatca agtcacgttg gccttgcgga
     1741 atagtgatat cggtccggct ggaggcttcg tgcgccatcc cctgtgcttc accgactaca
     1801 gccaccacca catcagattg tttcgccgtc tgcaccgctt catcaatcat ctcttgcggc
     1861 gaacgcggat cgactttgac cgcttcttca tactgattca ggaaatcgat aatgcctttg
     1921 tcactggtaa cgttcgcccc tttggcatac agcactttac cgttttcacc gacggaattt
     1981 ttaatcccgg tcagtacggt cacggattga tcggcaacac cggctgcgga ccagctgccc
     2041 atcacgtcac gtttactgtc cgccagtggc ccaaccaccg caatggtggc cgattttttc
     2101 agcggtaacg tttcgagacg gtttttcagc aacaccaggc tttcgcgcgc cacttcacgc
     2161 gcttctttac ggtgcaggcg gctttcggca ttggtatcca ccgggtcaga ctctttcggc
     2221 cccaaatggc tgtatgggtc gttaaacaac cccatatcat atttaacgtt cagtacatgg
     2281 cgggcagcat cgtccagctc tgccatggtc actttgccgg atttaatcaa cccaggcaga
     2341 tacttcgagt agtactcgtc gctcatactc atgttgattc cggatttcag cgccacgcgc
     2401 accgcatctt ccgggtctgc cgccgtgcca tgtttaatca gctctttgat tgcaccgtga
     2461 tcggaaacgg tgatgccttt aaagccccac tggtcgcgca gaacatcttt cagcagccag
     2521 gaatcggagg tggctggcgt gccgttcagc gagttcagcg ccaccatcac cgcgccgctg
     2581 cctgcgtcca gccccgcttt gtacggcggc atataatcat taaacaggcg ctgcggactc
     2641 atatcgacgg tgttgtactc tttaccgcct tctaccgcgc cgtatgcggc aaagtgtttg
     2701 acgctggtca tcaccgagta acgatctgcc gggcttttac cctgcatcgc ttccaccatg
     2761 gttttaccca ttattgaggt gagatacgta tcttcgccaa aaccttcgga agcacgtccc
     2821 cagcgcggat cgcgcgagac atcgaccatc ggtgcccagg tcatattcag gccatcatct
     2881 gccgcttcat aagcagagac acgcccgacc gttttcaccg catcgaggtt aaaagacgag
     2941 gccagaccga ggctaatcgg gaacaccgtg cgctgaccgt gcagcacgtc gtaagcaaag
     3001 aaaagaggaa ttttcaggcg gcttaattcc atcacctgat cctgcatggc gcggatatcc
     3061 tgacgggtta cggtgttgaa aatcgcccca acctgaccgt ctttgatcat ctcgcggatc
     3121 gcctctttcg gattatccgg gcctacgctg attaagcgca gctgaccaat tttctcatca
     3181 accgtcattt tcttgagcag atcggtgacg aacgcatccc gcgcttcagg cgttaatgga
     3241 tggttaccga ataaatcatc cgccagtgct ggctgcagga ccagactcac cgcgattcct
     3301 actgaacata gccatttcat atggattttt cttcctttgt tgcccgacgt ggcagcgaaa
     3361 atggtgcaaa aaccgtagtt tgccataagc atgatggaga gagaaaaaga atgctcagtt
     3421 tattgtctga attttcaaaa tattcactcg ctgaattgtc atacaaggcg ctattctagt
     3481 ttgtgatatt ttttcgccac cacaaggagt ggaaaatgtc ttccatgaca acaactgata
     3541 ataaagcctt tttgaatgaa cttgcccgtc tggtcggtca ttcacacctg ctcaccgatc
     3601 ccgcaaaaac ggcccgctat cgcaagggct tccgttctgg tcagggcgac gcgctggctg
     3661 tcgttttccc tggctcacta ctggaattgt ggcgggtgct gaaagcctgc gtcaccgccg
     3721 acaaaattat tctgatacag gctgccaata caggcctgac cgaaggatcg acgccaaacg
     3781 gtaacgacta tgatcgcgat atcgtgatca tcagcaccct gcgtctcgac aagctgcacg
     3841 ttctcggcaa gggcgaacag gtgctggcct atccgggcac cacgctctat tcgctggaaa
     3901 aagccctcaa accgctggga cgcgaaccgc attcggtgat tggatcatcg tgtataggcg
     3961 catcggtcat cggcggtatt tgtaacaact ccggcggctc gctggtgcaa cgtggcccgg
     4021 cgtataccga aatgtcgtta ttcgcgcgta tagatgaaga tggcaaactg acgctggtga
     4081 accatctggg gattgatctg ggcgaaacgc cggagcagat ccttagcaag ctggatgacg
     4141 atcgcatcaa agatgacgat gtgcgtcacg atggtcgtca cgcccacgat tatgactatg
     4201 tccaccgcgt tcgtgatatt gaagccgaca cgcccgcacg ttataacgcc gatccggatc
     4261 ggttatttga atcttctggt tgcgccggga agctggccgt ctttgcggta cgtcttgata
     4321 ccttcgaagc ggaaaaaaat cagcaggtgt tttatatcgg caccaaccag ccggaagtgc
     4381 tgaccgaaat ccgccgtcat attctggcta acttcgaaaa tctgccggtt gccggggaat
     4441 atatgcaccg ggatatctac gatattgcgg aaaaatacgg caaagacacc ttcctgatga
     4501 ttgataagtt aggcaccgac aagatgccgt tcttctttaa tctcaaggga cgcaccgatg
     4561 cgatgctgga gaaagtgaaa ttcttccgtc cgcattttac tgaccgtgca atgcaaaaat
     4621 tcggtcacct gttccccagc catttaccgc cgcgcatgaa aaactggcgc gataaatacg
     4681 agcatcatct gctgttaaaa atggcaggcg atggcgtcgg tgaagccaaa tcgtggctgg
     4741 tggattattt caaacaggcc gagggcgatt tctttgtctg tacgccggag gaaggtagca
     4801 aagcgttttt acaccgtttc gccgctgcgg gcgcagcaat tcgttatcag gcggtgcatt
     4861 ccgatgaagt cgaagacatt ctggcactgg atatcgctct gcggcgtaac gacaccgagt
     4921 ggtatgagca tttaccgccg gagatcgaca gccagctggt gcacaagctc tattacggcc
     4981 attttatgtg ctatgtcttc catcaggatt acatagtgaa aaaaggcgtg gatgtgcatg
     5041 cgttaaaaga acagatgctg gaactgctac agcagcgcgg cgcgcagtac cctgccgagc
     5101 ataacgtcgg tcatttgtat aaagcaccgg agacgttgca gaagttctat cgcgagaatg
     5161 atccgaccaa tagcatgaat ccggggatcg gtaaaaccag taaacggaaa aactggcagg
     5221 aagtggagta aaaattacgg atggcagagt atcgccatcc gaattcactt aatcgttctg
     5281 tgccgtctgc cccgccgccg ccatttgggc ggctttttgt tttttatagc tcaacgctgc
     5341 tgccggcaca ggcatcactt taccggtttc aatccaggta cgcaggcggc tggcatcggc
     5401 aaaatgggta tatttgccaa acgcgtccat cactaccagc gccaccggtt tattattgat
     5461 aaccgtacgc atcaccagac aatggcccgc cgcattggta aagccggttt tggttaactg
     5521 aatattccag ttatcgcgat acaccagatg attagtattg cggaacggca gcgtatacgt
     5581 cgggttagag aaggttgcca tatcttcccg ggtagtactt aactgcccga tcaacggata
     5641 ttgtttgctg gcaatgagca gtttggttaa gtcacgggca gttgaaacgt tatgcaccga
     5701 caatccggta ggttcaacaa agcgcgtgtt gttcattccg agcgatttcg ctttcgcatt
     5761 cattgcctta ataaaggctt tgtaaccacc gggataatgg tgcgcaaggc ttgccgccgc
     5821 gcggttttct gaagacatca gcgccagcaa cagcatatct ttacggctga tttcgctatt
     5881 cagtcgtacg cgcgaataga cccctttcat ctccggcgtc tggctgatat ccacttttag
     5941 tttttcatcc agcggcagtc gtgcatccag cacaaccatc gcggtcatta atttgctgat
     6001 agacgcaatc ggacgcacca gatccgggtg gttcgaatag atcactttgt tggtattcag
     6061 atcaacaatc atcgcgctac cggaggcaat ttccggttgt gaagcggtgg tagcggctgc
     6121 cgttttcgca acggcctgcg gtgcaaaagg cacagccagc atcagggcca agctaaataa
     6181 agaaactcga aatttcggca tgatgagcat tcagatagtg gttcacgcgc acgggttgcg
     6241 caccgccgga gtaaggattt actgaggcta gcgacgccat cataacgagc aaaaagtgcg
     6301 atcgtcaaag gagaatcgtg agtaaatgct gcattgctga catttacgcc agcaatgcaa
     6361 cgtcaaagaa ctttctagaa caaacgataa ccgtagcccc atagtataac ggttagggtg
     6421 agcagcactt ccagtaccag cacgccaatc gccagcgtcg aactggagaa gctaaggcct
     6481 tcctctttgt tgatattcag gaagctcgga ataccaaggt aaagcaggta gccggtgtaa
     6541 aacagcgcca ccgtgccgac cagcgcacac aaccagacca gtggataaag cgccaccaga
     6601 ccgcttaaaa acagcggagt tgcaacgtag cccgcgaaga ccatgcagtg cgcaagagac
     6661 ggacgctgcg ggtaattgcg cgccatccac cagatgaccc gccccatcac cgcaacccca
     6721 gccagcataa cgccgtaaaa caagacagcc agcgccagtc cggtaaacca ggataacttc
     6781 aggatagtgc catcgccaaa attccagcca atctgtgtag tgccaatgaa ggcgcaaatc
     6841 accggaatcg ccgccatcag caaaacgtgg tgggtgtaat ggtgagaaat cgtttcgttt
     6901 tcgcgattaa tcacctgcat ttcacgatcg ggatgggaaa acagtcccca gacatggctc
     6961 ataccgcccc cttgttgtga gttcatgaac ctgacagttc aagtataagc cagcttgtga
     7021 ttattttttg ttcatccatg taatttgcac cgtctgatgt atgtcctttt tcagcgtgaa
     7081 tgattcacag ggtgtatgct tacaggcaac caaagggaga caaactggcc tatggatctc
     7141 aatacactca tctcacaata tggttatgcc gcgctggtta tcggtagcct ggcggaaggt
     7201 gaaaccgtga ctttgctggg aggcgttgcg gcacatcagg ggctattaaa gttcccactg
     7261 gtggtacttt ctgtggcgct tggtggcatg attggcgacc aggtgctcta tctgtgcggg
     7321 cggcggtttg gcggcaagct gttacgccgt ttctcgaaac atcaggataa aattgagcgg
     7381 gcacagaaac ttatccaacg ccatccgtat ctgtttgtca ttggtacgcg ctttatgtat
     7441 ggctttcggg tgattggccc gacgctgatt ggtgccagcc agctgccgcc gaaaatcttt
     7501 ctgccgctga atattctcgg cgcatttgcc tgggcgttga tttttaccac tattggttac
     7561 gctggtggtc aggtgattgc gccgtggttg cacaatctcg accagcattt gaagcactgg
     7621 gtctggttga ttctggtcgt ggttctggtg gtgggcgtgc gctggtggct gaaacggcgc
     7681 gggaagaaaa agccggataa tcaggcgtaa agcagttgcc acagataagc attcactcca
     7741 catccggcac cgggtgttcc atgcctgatg cgacgctggt gtcttatctg gcctaaccca
     7801 ttgaaatatc cacgcacctc actgcgctat tctgggttga actgcggatt cgccaacata
     7861 aagccgccat ccactatcaa cgactgccct gtcgtgtaat ttgcgccctc cgaacaaagc
     7921 cacaccacca ggctggcaat ctcatgcgtt gcgccaaaac gccgtagagg aatcgaaggc
     7981 tccgcgtcgg gcttcacgtc gctatcatcc atgctgttca ttggcgtggc tatcgcccca
     8041 ggcgcgactg cgttcaccaa aatcttatgc ctgaccaact ccagcgccat cgttttggtt
     8101 aacccgccga gcgcatgttt agcggctgtg taggcgctgg catccggcag cggcgtatgt
     8161 tcatgtaccg acgtaatgtt gatgatgcga ccgccctgcc cttatttcac catctgacga
     8221 gccgcaattt gcgagcataa aaatgcacca tcaacatcaa cggtaaaaat cttgcgccac
     8281 tcatcaaaag ccatatcaag aaacggcgct ttggtcattg cacccgcatt attcaccagc
     8341 acatcaatac gcccaaatcg ctggatgagt gtctccagcg cctgcgcacc ttctggcaga
     8401 ttgccgagat ccagttgcac gatctccgca cgtacgccgt gggtaaccac ctcacgcgcg
     8461 gtatcttttg ccccttcttc atctgagtgc caggtaatgc caatatcaaa cccctgctgc
     8521 gccagtaata acgcgcactc tttgccgatc cccgaatcgg aagcggtaat tatcgcgacc
     8581 tatgccatcg agttctccac ttaacgctga ataaacgtta agtatagaag gcgcatatca
     8641 tcagcgtttg taacccccgc ccagcgcacc ggtgagtcga atggaggcat ccagccactg
     8701 cccttgcaat aacaggccat tggcccgctc acgcagcgcc gggattctgg cttcgctgac
     8761 gcgggaacca gcaatgatgc ccgcgttaaa gcgggcctgc gcaagaccta ccacacgcaa
     8821 ggcatcgcgc tcaatttgcg cctgatgctg gtttttctcc gccagtgtct gaacctgact
     8881 ggctgcccgc gccacgtcat tcactgcttc aaccaccgct ttgttgtagc tggcgataga
     8941 caagttgctt tcggcttttg cgatatcgag attggcgtta agacgaccac tatcgaaaat
     9001 aggtagcgtc aggcctgccg taacgcccat ttgctgcgcg gaatgacgga acagatcgct
     9061 taagtgcaac gcatcctgtt gcaggaaggc catcaggttg atgtcaggat aaaatgccgc
     9121 ttttgccgca tcaatagtgc ttaacgatga ctcaacgtac cagtgcgccg cctgcaaatc
     9181 tgcccgccgg gccagtaagg agtaccccag ttcatcagga agctggcttg ccactttcgg
     9241 caacgcgacc ggtttaagct tcaatgactt agtctggtga tttgtgagtg catttaaccg
     9301 tgcctcaata attttcattt tccccgcgac atcgttgagc tgctgccggg ttttgctggc
     9361 attaatatcg gtttccacac cgtcaactga agaagtaatc ccgttctgat atagctggcg
     9421 atcggtcgcg ataatggtgt tctgctcttt ttctatttgc tgcaagaccg tgtttaacgc
     9481 cgcctgggtt tgccactccc agtacaggcg ggctacgctg ccagccagca attggcgggt
     9541 ttgctcgcgt tccgccgccc gtgctttaac cgtacccagg cgggcagtaa cctccgcccg
     9601 attctttccc cagatatcga gatgccagcc cgccgttaag ccaaaagttc cgttggtgta
     9661 ccacggaccg gtcgtacctg cggccggatc gttcagagca aacggcccca ttagcccttc
     9721 tgccgacatt ttttgccgct ccatatccgc cgaaaagtcg atctgcggac catcctgagt
     9781 ggcaactgcc ttcgcctggg cttcagctag ctaaatgcgc tgttcagcca cctgcatatc
     9841 cggtgcgttc tgtagtgcat tgttaattaa tgaagtgagc tgattatcgt gatactccag
     9901 ccaccattgg ctgtctggcc aaccattttt cagcgccgtg ggtaatgcgg tgtcaacttg
     9961 tgcagcgggc gtttgctggc ttaacgcctg gcgggtttca tgcataggcg cacacccggc
    10021 cagcatcagt aacagcggaa aacaggcgat ggctggataa aaggaatcac gattcatggg
    10081 ggaataatca ggtaagaaac ggtgcgcgga gattaccgtg tgttgcgata tattttttag
    10141 tttcgcgtgg caatacatca gtagcaataa aacgacatac ccagaaaaat atagactaag
    10201 taaatgatat cttccgattt atcttaatcg tttatggata acggcaaagg gcttcgtttt
    10261 ttcctatact tattcagcac tcacaaataa aggaacgcca atgaaaatta tactctgggc
    10321 tgtattgatt attttcctga ttgggctact ggtggtgact ggcgtattta agatgatatt
    10381 ttaaaattaa ttaatgtcat cagatccgaa aataacgaga atatttcagt ctctcatcct
    10441 gttgcgctcc tgtcatgtgc attgcttcat ataatcactg gcgcaaggag cgcgcagggg
    10501 gcgaccaatc gccgccgccc cctgcccccc cgggctctgg cgaacaaaat cgccgctgcg
    10561 cggtgccctc ggcttatccc ttacggctac cgggtcgggc gcgaggtaac atccctgtaa
    10621 aacgcgccct cagcccacat ccatgtgggc tgccccggcc ttcagggaac gcctcggcga
    10681 ttttgacgcc accaaacaac cgtgcggcct attgataaag agctaacaca ttgtcaaaaa
    10741 catcactatg gtttttagag tttctcgata tcaattgcct gaatagccct tgcaatatca
    10801 ggggagttat tcaacacccg aacatgctga aataattccg ttgcttcatc gtattcttta
    10861 cgcaaataac tcaaccactg tttaatccgc gcaacgtgat ataacccggt atcgccctgc
    10921 ttttccagac gggtatattt ttgcagcaaa gcaaccacct ccggccacgg cattcgcggt
    10981 tcgttatatt ttaccacccg gctcaggttg ggaatattga gcgccccgcg accaatcatc
    11041 actgcgtcgc agccgctgat cgccatgcat tgttgcgcgc tctgccagtc ccagatttca
    11101 ccgttggcaa tcaccggaat attcagccgc tggcgaatct cgccaatcgc ctgccagtca
    11161 atatgctccg cgcggtaacc ctgctctttc gtccgcccat gcaccaccag ctccgtagcg
    11221 ccagcctgtt gaaccgcatc ggcgatttca aatttcttct caccgctgtc ccagcccaga
    11281 cgcactttca cgctgacagg caaatgcgcc ggtacagctt cacgcatcgc ttttgccccc
    11341 tggtagatga gttcaggatc tttgagtaac gtcgccccgc cgccgctacc gttaaccgtt
    11401 ttcgacgggc agccgcaatt gagatccacg ccccagaaac ctaactccac cgcacgggcg
    11461 gcgttctctg ccagccattg tgggaactga cctaacaact gcacgcgcac cagcgtacca
    11521 gatggtgtcc ggctggcgtt ttgtagctca gggcaaatgc gatgaaagac ttttaccggc
    11581 agcagttgat ccaccacgcg gacaaactcg gtgatgcaca gatcgtagtc gttaacttcg
    11641 gtcagcaatt cacgcaccag agagtcaagc actccctcca tcggtgccag taacacacgc
    11701 atatcatcac ccgcaaaaaa atgaggcgct atgttagcgc ctatggtcag cggattaaag
    11761 gtctgcaaag agggattgct gcaaactgca atccctctct atgcttgatg tatgcctgag
    11821 ttcgctgctc attgcgtaat accgggtagc gaaaaaataa gcgctgattt cttaatgtga
    11881 tcggtagcac gttttacgaa ttaattgtat gatgaatcca tctcatctgg ggtgttgatt
    11941 atgagtaaga cactgaacat tatctggcaa tatttacgcg ctttcgtcct gatttatgcc
    12001 tgcctgtatg caggcatttt cattgcttcc ctgctaccgg taaccattcc gggcagcatc
    12061 atcgggatgc tgatcctgtt tgtcctgctg gccttgcaaa ttcttccggc gaaatgggtc
    12121 aatccggggt gctacgtact gattcgctat atggcgctat tgtttgtgcc gattggcgta
    12181 ggcgtcatgc aatattttga tttgctccgc gcgcagtttg gcccggtagt ggtttcctgt
    12241 gctgtcagta cgctggtggt ttttctggtg gtgagctgga gttcacaact ggtacacggt
    12301 gaacgtaaag tcgtaggtca gaaaggatca gaagaatgat ggcgaatatc tggtggtcat
    12361 taccgctgac gttgattgtc ttttttgccg cccgcaaact ggcggcacgg tataagtttc
    12421 ccctgcttaa cccgttgctg gtagcaatgg tggtgatcat tcctttttta atgctgaccg
    12481 gcatctctta cgacagttac tttaaaggta gcgaagtgct aaacgatctg ctgcaaccgg
    12541 cggtcgtcgc gctggcctat cctttatatg agcagctaca ccagatccgc gcgcgctgga
    12601 aatcgatcat caccatctgt tttatcggca gcgtggttgc gatggtgacg gggacttccg
    12661 tggcattgtt gatgggcgct tcaccggaaa tcgccgcgtc aatcctgccg aaatcagtca
    12721 ccacgcctat tgcaatggcg gttggcggca gtattggcgg tattccggca atcagcgcag
    12781 tttgcgtgat tttcgtcggc atcctcggcg cggtatttgg ccatacattg cttaatgcga
    12841 tgcgtattcg taccaaagct gcgcgcggtc tggcaatggg gactgcctcg cacgccctcg
    12901 gtacggcgcg ctgcgccgag ctggattatc aggaaggtgc atttagttcg ctagcgctgg
    12961 tgttatgcgg gataattact tcgctgatcg caccgttcct tttcccgatt attctggcag
    13021 taatgggcta aaatttgcga tacgtcgcgc atttttgatg taagtttcac gtgttgcata
    13081 attaatgaga ttcagatcac atataaagcc ataacgggtt cgtaaactgt tatcccatta
    13141 catgattatg aggcaacgcc atgcatccac gttttcaaac cgcttttgcc caacttgcgg
    13201 ataacttgca atctgcactg gaacctattc tggcagacaa gtacttcccc gctttgttga
    13261 ccggggagca agtctcatcg ctgaagagcg caacgggact ggacgaagac gcgctggcat
    13321 tcgcactact tccgctggcg gcggcctgtg cgcgtacgcc attgtcgaat tttaatgttg
    13381 gcgcaattgc gcgcggtgtg agcggaacct ggtattttgg tgccaatatg gaatttattg
    13441 gtgcaacaat gcagcaaacc gttcatgccg aacaaagcgc gatcagccac gcttggttga
    13501 gtggtgaaaa agcgcttgca gccatcaccg ttaactatac gccttgtggt cactgccgtc
    13561 agtttatgaa tgaactgaac agcggtctgg atctgcgtat tcatttgccg ggccgcgagg
    13621 cacacgcgct gcgtgactat ctgccggatg cctttgggcc gaaagatctg gagattaaaa
    13681 cgctgctgat ggacgagcag gatcacggct atgcgctgac tggtgatgcg ctttctcagg
    13741 cggcgattgc agcggcaaac cgttcgcaca tgccttacag taagtcacca agcggtgtcg
    13801 cgctggaatg taaaga
//
LOCUS       DADLTY010000110        13394 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.110, whole genome shotgun sequence.
ACCESSION   DADLTY010000110 DADLTY010000000
VERSION     DADLTY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.110"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..1274)
                     /locus_tag="JN334_002771"
     CDS             complement(<1..1274)
                     /locus_tag="JN334_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7438860.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEA"
     gene            complement(1299..2336)
                     /gene="ypdE"
                     /locus_tag="JN334_002772"
     CDS             complement(1299..2336)
                     /gene="ypdE"
                     /locus_tag="JN334_002772"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY7438861.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(2336..3419)
                     /gene="ypdF"
                     /locus_tag="JN334_002773"
                     /pseudo
     CDS             complement(2336..3419)
                     /gene="ypdF"
                     /locus_tag="JN334_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(3434..4681)
                     /locus_tag="JN334_002774"
     CDS             complement(3434..4681)
                     /locus_tag="JN334_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAY7438862.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(4703..5029)
                     /locus_tag="JN334_002775"
     CDS             complement(4703..5029)
                     /locus_tag="JN334_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HAY7438863.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(5248..6213)
                     /gene="glk"
                     /locus_tag="JN334_002776"
     CDS             complement(5248..6213)
                     /gene="glk"
                     /locus_tag="JN334_002776"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAY7438864.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            6417..7673
                     /locus_tag="JN334_002777"
     CDS             6417..7673
                     /locus_tag="JN334_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HAY7438865.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            7788..8114
                     /locus_tag="JN334_002778"
     CDS             7788..8114
                     /locus_tag="JN334_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY7438866.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(8255..9493)
                     /gene="mntH"
                     /locus_tag="JN334_002779"
     CDS             complement(8255..9493)
                     /gene="mntH"
                     /locus_tag="JN334_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HAY7438867.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            9829..11031
                     /gene="nupC"
                     /locus_tag="JN334_002780"
     CDS             9829..11031
                     /gene="nupC"
                     /locus_tag="JN334_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HAY7438868.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(11081..13193)
                     /locus_tag="JN334_002781"
                     /pseudo
     CDS             complement(11081..13193)
                     /locus_tag="JN334_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
BASE COUNT         3325 a         3646 c         3313 g         3110 t
ORIGIN      
        1 gcttcaaatt gctcctgctc gtccggcgcg ctgtcgcgat ccatatagag catttcggta
       61 cggaacagcc cgacaccttc cgcgccgttg gcaaacgcgc ctggcgcttc cagagcggtg
      121 ccgatattcg ccgcaatatc aatacgcttg ttatcacggg aataggccag ctgcgcggca
      181 gcctgcgcct gttgtttttg gcgtttatcc gccagcgtct gcgcgacctg ataataaccg
      241 ctcaccgcgt cattcgggtt aatcgccagc acgccgcact gggcgtcaag cactgcaggt
      301 tgcccggcat aacgggcaat cgcatccagc ggcaagccac tcagtaccgg gatcgccgag
      361 gcacgggcca gaatcagtgt atgcgaggtg cgcccggttt tctccagaat catacccgca
      421 agatttttca aatcaaggct caaaaactgg cttggggtta aatcttcagc caccagaatg
      481 gtcggttttt ccagcaccag attgttgcgc ggcttcagtt ccggccaggt gatatgcagc
      541 aactgttcgc tgatatcgcg aatgtcgctg acccgctcgc gcagataatc gctggcagaa
      601 gcagatagtt tggcgcaaac ctgctccata ttgctgatga tcgccgcccc cagtccctga
      661 tgctgttctg tcatcaggcg acggatattg cctgcaaatt catcatcctg aatcagcgac
      721 aaatgagcgc tgaggatagt tttgctttcg ccgtcacgct cacgcaattg ctgattcagt
      781 tgctcggcaa gcgttgccag gctgtgctcc agccgggtgg aatcttgcgc actggcgggg
      841 attgcccgat aactgtcgag gctgtcgctt tgtaacaggg tcagcgtacc cacgccgacg
      901 ccgcttgcca gcacattgcc gtacagtaaa tccgggttaa ggcggcttaa tgaacgcggc
      961 agcggatgcg ccgtcagttc tgcctgcgtc ggctgaacgc tgtcgctgtc gataaagcgc
     1021 accaggatgt actcttccag cacgcgccgc gcctgctctt catcgctgcc gctaatgttc
     1081 aggctgcaac tgtcattaaa taaggtgcca gttccaatca acgccagcga gcttttagca
     1141 tcagcctttg cgttctggcg atggttgata aaggtgattt cgctttgcca ctggctgcac
     1201 tgttctttaa gttcccacgc cggacgagca tgtagaccgt taggcaaagg acagagaaat
     1261 tgaatcgtta acataaccgc tccgggttaa tcaggagatc atctgaaatc cgtcagttga
     1321 acaaccgtct cacgcgtaag acgttgaata agggcagata aaagttgctc catctgcaaa
     1381 atgtcgcggc aatcggcaat cgatgccgcg caatgtccgt ggcgggtggc aggccccatt
     1441 accaatgtgg gtacgccagt accggttaag tgcaccgctc caccgtccgt gccgccgtta
     1501 ctgaacatat ccgcctgcaa cggaacgcca atttctgccg ccacggtttc aatccacgcg
     1561 gtaagttttg gcggcgcaat cagcgattta tcgcttaaca ccagcatcgg cccgttacca
     1621 atctgacgat ggttagccgc gccataatca aagtttttcg cccagcaggc ggtatcaagc
     1681 acaatggcga cgtccggcga caccgcgcgg gtagcagttt gcccgccgcg taatcccacc
     1741 tcttcgctgg aacttgccac cagccacact tccgcaggca gttcggcgtc gtgcagctca
     1801 cgtagtaacg tcaccagcag atagcaactg aggcgatcat caaaggcttt gcccatcacc
     1861 cgctggtgag ggaggacctg aaaagtggta tcgaaggtga cgcgatcgcc tggacgaatt
     1921 cccgcctgca tcacttcgtc ataggtgcgc gcgccaatgt ccacgcgcat ggcgctaacg
     1981 tcattcccct gccggtcgcc gtcaagcagg cctggaattt tgcactcttc acgggtggtg
     2041 atgcgcaccg gctgcagctg gcgggcagcc atgcgcacgt tgccaaccgg cagcacgtca
     2101 atagcgcctt cgcgggagat gctgcgcacc ataaatccca cttcgtccat atgggcgcag
     2161 atcatcacct tcggacctgt cgattcattg aggcggatca gcaccgatcc cagaccatca
     2221 aatcgcactt ctttttgcag gcgagccgct tcttccagca ggatctgccg cacttcctgt
     2281 tccgaggagg cgatcgcatc tgcctcgctc aacgctttta atagcgataa atccattatg
     2341 cctctcccgt gagcaacact gttttcggca tggcgtagag cacttctgcg ccttgcgggg
     2401 taaccagcac aacatcttcg atgcgcacgc ccccttgccc tagcaaataa atccccggct
     2461 ccacggtcag caacatgcct ggctgtagcg tcgtggtgtc ccgcggtgaa aaacgcggat
     2521 cttcatggac ttcaatgccg atagcgtgac cagtgttatg accgaaatag tcgccaaatc
     2581 cagcctcggt aatgacccgg cgcgcggcgt cgtcaatctg ctggcagcgc acgccggggc
     2641 gaattgcaga gattgccgcg agctgtgcct gcaggacaat ctgatagacg tcaaacagcg
     2701 ggtgagattc ggcgctcacc ccttcgccat tcaccagcaa ggtgcgcgtc atatcagcag
     2761 tagccctgat acagcgcacc aaaatcgaga gtgacaaact cgcccgctgc aacaatcttg
     2821 tcgctggctt tgccgtgcgg cagcgccccg cgccagccgc tggcgacaat ggtgtcaaaa
     2881 gaggcttttt ctgcgccctg ctggcgcata aaccactcca gttcagcggc tatctcgcgc
     2941 tcgctcatcc ccgcctgaat aaagcggcga atatgctccg ctccgcgatc ggcaatccca
     3001 caggcgaggc ggattatctc cacctcctct ggcgttttga tttgccgcag cacatccggc
     3061 gtggcgctaa ccagtttcgc attgagttca gacttccagc ggtgcgcggt ttcccaactc
     3121 acctgctggc cttcaaaacc gagcgtttgc aactgctcat cggcaatgat ttgattgacg
     3181 atagtggtaa gcgtgttcgt tgcgtcaagc aaatgcagct ggtagccttg cgcacgggct
     3241 tccacctcaa cgaaatagcg cgaatctacc agaatgtgcg cactttcacg gctaataacc
     3301 acatagcctg atccggtgga gatccccaga tgcggctgtt tgttctgccg tgaggagaga
     3361 agcaccgcat ccagttgttg caccttaagc cagttgcgca gcgaagcgag taatgtcatc
     3421 tggattttcc ttcttacagg ctatcgatta acaatttgcc tttgcggaac atcatcagac
     3481 gcaggaacac caccatcagc gcagtaatga ttgcccccag cgcgatcccc gccatataaa
     3541 cgcccaagtt agtgaccagc ggccatgccc agatagcgga ttccgggaac cattgcactg
     3601 cgcccagcca gacagctgcg gttgagccga caatcgcgcc gaccatatat gacggaatag
     3661 cggtgatggg gctttccagc gcaaacggaa tcgcaccctc actaatcccc ataaaggcca
     3721 ggaacatcgc ggtcttaccc tgcggataaa gttgagcgtt gaacaggcgt ttgccggtta
     3781 aacgacggtc aataatggtc gccagaccca gaccaatcgg cggaataacg atagcgatag
     3841 aacgagcggt gaccggtaat acgtggtcag tggtaaagct gaaggcaacg aaacctgcgg
     3901 ctttgttgat cgggccacca aggtcgattg ctgtcgccgc ggcgatcccc attgcgtaca
     3961 tcagcgcgcc cttctcacct gcggcagtca gcacagtacg gataccgccg ttgatccagc
     4021 caccaaaggg agtgatgacg tagtacatcg ccagcatgac aaaaatggcg gaaagaatcg
     4081 gtagcaaaaa tgtggttttg aacgctaaca ggaaatccgg taactggatt ttctggttca
     4141 tccatttcac aaggtaaccg gcaacgatag aaataatcag cgcaccaatg aaggtggacg
     4201 gcaccggcga agaggtcgcc cattgcatag tgctgggatc gaagttcagc agttgcgtcg
     4261 gctgggtaga catcaacccg ccgataaaac cagccgggaa cgccagtttg ccaccgatag
     4321 agttcgccac aaatgcagca aacatcggaa tggcaaaacc aaacaacacg ccgccgaagg
     4381 actgcgacag ccaggcaaat ttaagtaagg agaggtcgaa accggagaat ttcccgctat
     4441 taagagcatc catgatgccg atctcagcag gaattttaag ccagctataa gcaatcagct
     4501 ggctgaaagc gaggatcaca ccgcccataa ttaaggtcgg caccatgcgt gaaatccctg
     4561 acatcacatg ctgcgggagt tcaccccaga agctgctttt agaggcctgc ggattcttaa
     4621 ctgccgctgc cgcaccggat gcgccaggca caacggttgc actgcgtttt ttaatggcca
     4681 taacaattat ccctgtcgaa tattactgtt gttcagaggc aatcatctct tcgatttctt
     4741 tgatgatgcc cgcagcgttt ttaattgcgt cctgcaaagt gatttcataa acgtcgcgtg
     4801 attcgaaacg ttcgttatct tccggggtaa ctgccacgga atggatgatg atggtcgctt
     4861 cggcgatatc ctgcgccgtc aggcgattct ggatgccgtc cgcgccctgg gtttcaattt
     4921 tcacttcata accggcttct accgccgctt cttccagcac ctgagcggcc ataaaagtgt
     4981 gagccaggcc cattgggcag gcacataagg caatcagttt cttactcatc gttaattcct
     5041 cgttcagtga tagttgacta gtgaaattat gaacgtgcaa aacaaatcgc cgttaccaga
     5101 caaataatgc atttactgga gaattaatcc cgctgtacgg aagtgtgacg caggtcggcc
     5161 ttgtgagggg acacaaagaa aaattgaatt aactgtatga tttaaaagat tatcgggaga
     5221 gttacctccc gatataacag gaaggattta cagaatgtga cctaaggtct ggcgtaaatg
     5281 tgcaccggaa ccgagaagac ccggatagtc atggacgatg agatacaccg gaatatcatg
     5341 gacatattct ttaaagcgcc ctttatcttc aaatgcggca cggaaaccgg aggctttgaa
     5401 gaactcaagg aagcgcggca cgataccgcc cgcaataaac acgccgccaa atgtcccgag
     5461 attgagcgcc agattgccgc caaaacggcc cataatgacg caaaacagcg acaatgcgcg
     5521 gcggcaatcg gtgcagctgt cagccagcgc gcgttcggta atatcttttg gcttgagatt
     5581 ttctggcagg cggttgtcag ctttcacaat tgcgcgatac aaattcacca gcccagggcc
     5641 agaaagcacg cgctccgccg aaacatgacc aatttccgca cgcaatattt cgaggataat
     5701 ggcctcttct tcactattcg gcgcaaaatc aacgtgaccg ccttcgcccg gcaagcttac
     5761 ccaacgctta tcgacatgga ccagatgcgc aaccccaagc cccgttccgg caccgtaaac
     5821 cgcaataggc ttaccttcga ccggttctgc gccaccaaac tgaatcagat gctctttttt
     5881 cagcatcggg atcgccatcg atacagcggt aaaatcgtta ataatttcca gatggctaaa
     5941 accgagattc tttttcattt cggcaattga gaacgcccag gtatggttgg tcattgccac
     6001 ccagtcaccg gtaattgggc aagcgatggc aatacagccg tctttcacct cgaccttatg
     6061 ttcttcaaga taaacgcgaa tgaccgcttc gaggctgggg taatcaagcc ctgaataggt
     6121 cttagcctgc gagatttcac cactggcaat atcacacaga gcaagacgtg cgttggtgcc
     6181 gcccacatca ccgactaatg catactttgt cattcttcaa ctgctccgct aaagtcaaaa
     6241 taattctttc tcacactgta aatacctggg ggcataacaa caacgccgga aaggcaggct
     6301 ccctgtaaat atcgatctgg gtcacacaat tactttatcg tttcagcacc aattgcagcg
     6361 atgccttttt gcaagctggg caaactaagt atctgacccc gcataaggaa tagaacatgc
     6421 tccatccgcg agccagaacc atgttgttat tatcgctccc cgccgtggca attgggattg
     6481 cgtccagtct tattctgatt gtggtgatga aaatcgcctc ggtattacag aatttgctct
     6541 ggcaacgact gccgggaact ctggggatag cccaggattc acccctctgg atcatcggtg
     6601 tattaacgct aacgggtatt gcggtggggt tggttatccg tttcagccag ggtcatgccg
     6661 gaccagaccc cgcctgtgaa ccgctgatcg gcgcaccggt tccgccctct gcgctaccta
     6721 gacttatcgt agcattaatt ctcggtcttg ctggcggcgt cagcctgggg ccggaacatc
     6781 cgatcatgac cgtcaatatc gcccttgccg tcgccattgg cgctcgtctg ttaccgcgcg
     6841 tcaaccgaat ggagtggact attttagcct ctgccggaac catcggcgcg ctgtttggta
     6901 cacctgttgc ggcggcgttg atattttcgc aaaccttaaa tggcagtagt gaagttccgc
     6961 tatgggatcg tctttttgcg ccgttaatgg cggcagcagc tggtgcactt actaccggat
     7021 tatttttcca tccccatttt tcactgccca ttgcgcatta cggacagatg gaaatgaccg
     7081 atattctcag cggtgcaatt gtcgcggcga ttgccatcgc agcagggatg gtcgccgtat
     7141 ggtgcttacc acggttgcac gcgatgatga atcaaatgaa aaatccggtg ctcgtgctgg
     7201 gtattggcgg atttattctc ggaattctgg gggttattgg tggaccagtt tcgctgttta
     7261 aagggctgga tgagatgcag cagatggtgg caaatcaggc tttcagcacc agcgattact
     7321 ttttgctggc ggtaattaaa cttgccgccc tggtcgttgc tgccgccagt ggctttcgcg
     7381 gtgggcgaat cttcccggca gtgtttgtcg gcgtggcatt agggttgatg ctgcatgagc
     7441 acgttcccgc cgttccagcg gcaataaccg tttcctgcgc tattctcggc atcgtgctgg
     7501 tggtaacacg cgatggctgg ttaagtcttt ttatggcggc agtcgttgta cccaatacca
     7561 cattgctacc gctgctctgt atcgtcatgc ttccggcatg gctgttatta gcaggtaagc
     7621 cgatgatgat ggtcaatcgt ccgaagcaac agccacccca cgataatgtt tagcaaaaaa
     7681 tgctctcctt tatgatttaa gagttatggc gatgatacgg ggctttacct ccccgtaata
     7741 ttgcgttaac aggccgctga cggcttcgat cgtgaaggag aataacaatg ttcaggtcac
     7801 tgtttctggc ggccgccctg atggcattta ccccgcttgc agcaaatgca ggtgaaatca
     7861 ccctactgcc atcgatcaaa ttacaaattg gcgatcgcga tcattacggt aattactggg
     7921 acggtggtca ctggcgagat cgtgattact ggcatcgcaa ttatgaatgg cgcaaaaacc
     7981 gctggtggcg tcatgataat ggctatcatc gtggctggga taagcgtaaa gcatatgagc
     8041 gtggctatcg tgaaggctgg cgcgatcgtg acgatcatcg cggaaaaggc cgcggacatg
     8101 ggcaccgcca ttaaacgcgt cctccaatgg agcacaatgc ctgatgcgac gctagcgcgt
     8161 cttatcaggc ctacaaaccg gctctttaac gcgtagggcg gataaggcgt ttacgccgca
     8221 tccgccaata gtgccagatg cgacgctcat tcaactacaa tcccagcgcc gtccccacca
     8281 acaaccagat attcagcgcc acgaccagca ctacaatcac ccagcctgtc tgttttacgc
     8341 gtttgctgtt caccagatcg cccatcaact tgctgtcact ggtgaaaatc agcagtggaa
     8401 ccagcgccag ggcgatacca aaacttaaca gcacctgact cataaccaga atccgtgtcg
     8461 gatctaatcc catcagaatg acaataaatg acggcaacat ggtgactgta cgacgcaccc
     8521 acagcgggat atggaagcga atgaatccct gcatcaccac ctgccccgcc agtgtcccca
     8581 ccaccgttga ggacagtccg gcagcaacca gacttaaccc aaagaccgtt gccgcagcat
     8641 ggcttaacag cggttgcagc gtcagataag cctcatcaag atcggcaaca ccagtatgac
     8701 cagaaaagtg gaacgccgcc gcagctgtag ccatcatcac cagattgaca aaaccggcaa
     8761 tcgtcatggc gatagccaca tcccatttgg tggcggaata acgttgttga cgcgaaccgc
     8821 catgtaaatg ctgagtgagc gaagagtgca aataaatcac atgcggcata atcgtcgccc
     8881 ctaacacgcc tgctgccagg aagaccgcct ccgaagtagg taaactcggg atcaccattc
     8941 ctttacccag ctgcgccagg ttaggctggg agaaaatcaa ctcgacaatg taagccgcgg
     9001 caacaaacaa cagtaacccg ccaatcactt tctccagcgg tttttgcccg cgacgttgca
     9061 gcattaaaat caggaaagtc gcgatccccg tcagcaccgc gccctgcaac aacgaaacac
     9121 caagaatgag tttaaaaccg atcgccgcac caataaattc cgccaggtcg gttgccatcg
     9181 caataatttc tgcctgaacc caatagaacc acactacggg acgcggatag tgatcacgaa
     9241 tctgctccgc cagattttta ccggtggcaa tccctagttt ggcagagagg atctgaatca
     9301 gcatcgccat caggttggcc caaacgacaa cccacagtag ctgatagccg aagctagcac
     9361 ccgcctgaat attggtcgca aagttaccgg gatcgatata accaatcgcc gcaatgaacg
     9421 caggtcccat taatgcgagc ctcatcttac gcgccgcccg tccgctgcta ctctcaacgc
     9481 gatagttcgt catcttgtgc ctctaaaaca tagcctttgc tatgtttcat gctatgctaa
     9541 acgagaatga ttatcaaatt catttaaatg gattgtggtg atttctctga tagaccaaga
     9601 ttatgattac gaaaagattg atgacttgaa tgttatggtt atgtttaatg ttagcacatt
     9661 tacataactt tcagcttcca tacacaacat agcagaaatg tatgacagat cactattttt
     9721 gaagcctgtc acaggacgtc attatagtgt gtgtcagatc tcgttttcct taaccatgtt
     9781 acatagaatg tgcacggaaa tttaacctgc ctcatatttg gagcaaatat ggaccgcgtc
     9841 cttcattttg tactggcact tgccgttgtt gtgattctcg cactgctggt aagcagcgac
     9901 cgcaaaaaaa ttcgtatccg ttatgttatt caactgcttg ttatcgaagt gttactggcg
     9961 tggttcttcc tgaactccga cgttggttta ggcttcgtga aaggcttctc cgaaatgttc
    10021 gaaaaactgc tcggatttgc caacgaaggg actaacttcg tctttggtag catgaatgat
    10081 caaggcctgg cattcttctt cctgaaagtg ctgtgcccaa tcgtctttat ctctgcactg
    10141 atcggtattc tccagcacat tcgcgtgttg ccggtgatta tccgcgcaat tggtttcctg
    10201 ctctccaaag tcaacggtat gggcaaactg gaatccttta acgccgtcag ctccctgatt
    10261 ctgggtcagt cagaaaactt tattgcctat aaagatatcc tcggcaaaat ctcccgtaat
    10321 cgtatgtaca ccatggctgc cacggcaatg tccaccgtgt cgatgtccat cgttggtgca
    10381 tacatgacca tgctggaacc gaaatacgtc gttgctgcgc tggtactgaa catgttcagc
    10441 acctttatcg tgctgtcgct gatcaatcct taccgtgttg atgccagtga agaaaacatc
    10501 cagatgtcca acctgcacga aggtcagagc ttcttcgaaa tgctgggtga atacattctg
    10561 gcaggtttca aagttgccat tatcgttgcc gcgatgctga tcggctttat cgccctgatc
    10621 gccgcgctga acgcactgtt tgccaccgtt actggctggt ttggctacag catctccttc
    10681 cagggcatcc tgggctacat cttctatccg attgcatggg tgatgggtgt tccttccagt
    10741 gaagcactgc aagtgggcag tatcatggcg accaaactgg tttccaacga gttcgttgcg
    10801 atgatggatc tgcagaaaat tgcttccacg ctctctccgc gtgctgaagg catcatctct
    10861 gtgttcctag tttccttcgc taacttctct tcaatcggga ttatcgcagg tgcagttaaa
    10921 ggcctgaatg aagagcaagg taacgtggtt tctcgcttcg gtctgaaact ggtttacggc
    10981 tctaccctgg tgagtgtgct gtctgcgtca atcgcagcac tggtgctgta aaaccataca
    11041 taaaaaagcc ggggatcatt ccccggcttt tttacgcctg ttaatcagct aatggctgcg
    11101 ggcgaccaat caaataccct tgcagatatt gcaccccgag cttatgcaat agcgcctgct
    11161 gctgttgcgt ctcgacaaac tccgcgacca cactcaatga cttcgctttc gccagatcgg
    11221 taattgatct cacaatcatc gcatccagcg tattcgtgac aatatctttc acaaagacgc
    11281 catcaatttt gatgatatca gcctgcaaac gctttaaccg ttcgtagttg gcatatccgg
    11341 tgccaaagtc atcaatcgca atccggaaac caaacttatg cagctgctcg atgttgtaca
    11401 tgctgctttc tgcgttagaa aacgcctgct cctcggtgat ctcaagaatg accgcctgcg
    11461 gggagatgtg ataacgctta aacagacgaa taatccgccc ggcaatattc ttttgcagca
    11521 gcgtgagcgg cattaaattg actgaaaagc gcggtccttt tttgtcgcaa gggtgtgttg
    11581 ccagccactt caacaaggat tccagcactt gcaaatcaaa acgcgcgcta aggttaaact
    11641 gagcaataag gggcagaaac ttatccgggg tcataatgcc gccgtcatat ttcagtcgcg
    11701 cgaggatctc atcataacct tcaccctctt tgttgcgaat tggctgggcg tagagcagca
    11761 aatcaccctg atctaacgcc gtgcgaatgg tattcagcaa tagcacctgt ttagtggtct
    11821 gcccggaaac catctcctct ctgctatcca gcgccagcac atgatgatgt gcgcaggatt
    11881 gctccgccag ccagcttaac tgccccaaca agggttgcag ggtttcctga tttccatcaa
    11941 aacgccccca ggcagcacca tagcccatat ccagcccggt attgttccag tgaatttgcc
    12001 gactattcag gatgttaacc atatgctgga gtcgcccttc cgtttccggc ccgctcagca
    12061 ctaacagcag ttcactaccc ggcaattgat acaacttttc gttttcctgc atcaacggca
    12121 gcagcgtacg gcaaattgag cggatacagt gaacgcgcat cattaagccg taatgacgac
    12181 tcataaactc aagattatca atgcgcaggc agcaaaaact cttgcccgcc tcttgctccg
    12241 gcgcttgttc caacgcacga aagttgggta ggagcgttaa cggatctgtt agcgcctgca
    12301 aatgccagcg gcgattaagc cattcactgc gatgataaat gcgcaccata tagagcaggc
    12361 aaacgctaaa ggaaatcagc accgcgagaa taaatgccag cgaatattcg gtttccaccc
    12421 cttgcaaaaa gttctgattg taattcagaa ggcaaagcgt tgaaacagcc caggtgagat
    12481 ttaaaaacgg atagcgaaga ttaccgaccc cgagggtaaa gatgatgaag aaaacgggta
    12541 ccaggtaacc ggcaataaag tcgttttcat aaggtgtgca caacagaagt agcagcacgc
    12601 ttaacgctgc cagccagctt aaggtaaatg cgagtgagat agtagaaaag catgttgtaa
    12661 atcagcactg cggtgaaaag gcttagtaaa tcaacgaccg tgaaaatggc atccgcatcg
    12721 ccgaaaaagg tggatatctt tagcgggaaa tcaaagaagc tccccacaag atacatgctg
    12781 catttgatgc cgatcggcgt caccaaacca agccagaaca gacgttgcca gacatatcgg
    12841 ctggtcaatc cataacgcca acgcgtcccc agctgccagc gtaatattgc gcaggcacac
    12901 aacacgacaa acgtctggct gccgagcatg accgcagtct gtaaaaaaga gagatgaaag
    12961 ttccacagat tggtgcaaaa catcccgcat aaaacgggaa cgacaccgcg ccagccaaag
    13021 ataaacaaga ttgacagcat tacgcacagt ggcatccacg ccagaaagat ataactggaa
    13081 tggataatgg caagtggcga aataaaacgg gatagctgaa taagtaccac ggtgagcgta
    13141 aacgctagtg tgaatatctt gatatttttt atcaggttat gctccacaaa catgagtaca
    13201 ctactctcac tgatcggggt tttcaggcac acaaataata agtttattcg catcgcgaag
    13261 caaacagggg ccagcctgtt gccgattttt catccaatga tgctttcacc aggcggcgct
    13321 aacccggacg tagactgtat tacaaaagcg gcaaaaagca gagacaaaaa acccccgctt
    13381 tgcagcgagg gttg
//
LOCUS       DADLTY010000111        13249 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.111, whole genome shotgun sequence.
ACCESSION   DADLTY010000111 DADLTY010000000
VERSION     DADLTY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13249
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.111"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(16..999)
                     /gene="kdgT"
                     /locus_tag="JN334_002782"
     CDS             complement(16..999)
                     /gene="kdgT"
                     /locus_tag="JN334_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HAY7438869.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="JN334_002783"
     CDS             complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="JN334_002783"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HAY7438870.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2164..3198
                     /gene="rhaT"
                     /locus_tag="JN334_002784"
                     /pseudo
     CDS             2164..3198
                     /gene="rhaT"
                     /locus_tag="JN334_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3195..4133)
                     /gene="rhaR"
                     /locus_tag="JN334_002785"
     CDS             complement(3195..4133)
                     /gene="rhaR"
                     /locus_tag="JN334_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HAY7438871.1"
                     /translation="MAFCNNANLLNVFVRHIANNQLRSLAEVATVAHQLKLLKDDFFA
                     SDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADD
                     KHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVI
                     GQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAAS
                     LKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISD
                     ISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD"
     gene            complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="JN334_002786"
     CDS             complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="JN334_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HAY7438872.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5241..6710
                     /gene="rhaB"
                     /locus_tag="JN334_002787"
     CDS             5241..6710
                     /gene="rhaB"
                     /locus_tag="JN334_002787"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAY7438873.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6707..7966
                     /gene="rhaA"
                     /locus_tag="JN334_002788"
     CDS             6707..7966
                     /gene="rhaA"
                     /locus_tag="JN334_002788"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAY7438874.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8208..9032
                     /gene="rhaD"
                     /locus_tag="JN334_002789"
                     /pseudo
     CDS             8208..9032
                     /gene="rhaD"
                     /locus_tag="JN334_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9042..9356
                     /gene="rhaM"
                     /locus_tag="JN334_002790"
     CDS             9042..9356
                     /gene="rhaM"
                     /locus_tag="JN334_002790"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAY7438875.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9657..10103
                     /locus_tag="JN334_002791"
     CDS             9657..10103
                     /locus_tag="JN334_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAY7438876.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10114..11565
                     /locus_tag="JN334_002792"
     CDS             10114..11565
                     /locus_tag="JN334_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HAY7438877.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11555..12625
                     /locus_tag="JN334_002793"
     CDS             11555..12625
                     /locus_tag="JN334_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY7438878.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12625..>13249
                     /locus_tag="JN334_002794"
     CDS             12625..>13249
                     /locus_tag="JN334_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAY7438879.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3159 a         3459 c         3490 g         3141 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc
      601 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
      661 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
      721 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
      781 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
      841 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
      901 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
      961 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     1021 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
     1081 acattcattt aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt
     1141 tataggacca ttgtttttat tttggaattg atcacaaaaa aaccaccgcc gttggcgatg
     1201 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt
     1261 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     1321 tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     1381 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct
     1441 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     1501 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     1561 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     1621 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
     1681 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     1741 gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt
     1801 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     1861 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     1921 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     1981 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     2041 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     2101 gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag
     2161 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     2221 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     2281 gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     2341 gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     2401 ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg
     2461 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     2521 attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc
     2581 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     2641 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     2701 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     2761 gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc
     2821 atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     2881 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     2941 ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc
     3001 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     3061 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt
     3121 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc
     3181 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     3241 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     3301 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     3361 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     3421 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     3481 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     3541 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     3601 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
     3661 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     3721 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     3781 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     3841 atataaaaga gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca
     3901 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct
     3961 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     4021 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     4081 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
     4141 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     4201 actgtcgctg aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag
     4261 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     4321 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     4381 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     4441 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     4501 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     4561 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     4621 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     4681 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     4741 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     4801 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     4861 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga
     4921 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     4981 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa accacgtaaa
     5041 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
     5101 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
     5161 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     5221 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     5281 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     5341 aaatccatcg ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg
     5401 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     5461 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
     5521 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg
     5581 cacaacagca actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct
     5641 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     5701 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
     5761 gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg
     5821 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     5881 gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
     5941 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
     6001 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     6061 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     6121 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
     6181 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
     6241 ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc
     6301 gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca
     6361 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
     6421 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
     6481 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
     6541 tcggcatcga gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     6601 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     6661 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     6721 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
     6781 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     6841 ttccggtttt gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta
     6901 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
     6961 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     7021 ctcgcgcgac cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
     7081 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
     7141 tacgctctcg catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
     7201 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
     7261 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
     7321 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
     7381 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
     7441 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc
     7501 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct
     7561 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     7621 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
     7681 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat
     7741 gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc
     7801 ggcgggcgat tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgcggca
     7861 ggcggtctgg gaaatgtatt gccaacatca cgatacgcca gcaggtagcg aatggctgga
     7921 gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatgc
     7981 ggcacgagcg ccttatccgg cctacgggtc gaatgtccat gcgggctgtc accctcaccc
     8041 cggccctctc cctgaaaggg cgagggagaa aaacatagcc tcgtgcggca acggagctga
     8101 acaccgctcc ggacagtccc ctcgccccta cggggagagg gttagggtga ggggaacaag
     8161 ccgcaccatg tctaaacaag gcaacctaat ttacaggaac acagaacatg caaaacatta
     8221 cccagtcctg gtttgtccag ggaatgatca aagccaccac cgatgcctag ctaaaaggct
     8281 gggacgagcg caacggcggc aacctgacgc tgcgcctgga tgacgccgat atcgcgcttt
     8341 atcacgacaa cttccaccca caaccgcgct atattccgct cagccaaccc atgcctttac
     8401 tggcaaatac gccgtttatt gtcaccggct ccggcaaatt cttccgtaac gtccagctcg
     8461 atcctgcagc taacttaggc gtcgtaaaag tcgacagcga tggcgcgggc taccatattc
     8521 tctgggggtt aaccaacgaa gccgttccaa cttctgaact tccggctcac tttctctccc
     8581 actgcgagcg cattaaagcc accaacggta aagatcgggt gatcatgcac tgccacgcca
     8641 ccaacctgat cgccctcacc tatgtactgg aaaacgacac cgcggtcttt actcgccaac
     8701 tgtgggaagg cagcaccgaa tgtctggtag tattcccgga tggcgttggc attctgccgt
     8761 ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc acaagagatg caaaaacatt
     8821 cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg accgacgctg gatgaagcct
     8881 ttggtttaat cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt tattcgatgg
     8941 gcggcatgaa acagaccatc agccgtgaag agttgatagc gctcggcaag cgcttcggcg
     9001 ttacaccact agccagtgcg ctggcgctgt aaggagcaaa catgatccgc aaagcctttg
     9061 tcatgcaggt aaaccccgac gcccacgaag agtatcagcg tcggcataat cccatctggc
     9121 cggaactgga agcagtgttg aaatctcacg gtgtgcataa ctacgccatt tacctcgaca
     9181 aagcgcgtaa tctgctgttt gccatggtag agattgaatc tgaagaacgg tggaatgcgg
     9241 tagccagtac tgatgtttgc caacgttggt ggaaatatat gaccgatgtt atgcccgcta
     9301 acccagataa cagcccggtg agtagcgagc tgcaagaagt gttttacctg ccgtaattgc
     9361 ccaatgtgcc ggagatgcct ccggcacaat attttaaatt tcgatttgaa aaattaaaca
     9421 tattacatct cccggtaagt tatatttccc tgatacattg tgagtaaatc acaaaaataa
     9481 tgaataaccc attaatgatt catgtggttt atttaaataa ccattatgtg cattactccg
     9541 caaatctgac ctttcactct gtctcaaatt gtcattgttt accgacatat cgcctctcat
     9601 cacttaatcg ctccaccatt acaattgatt aaaatatatt ttatggagtc cgattaatgg
     9661 cagctcttac tgcaagctgt attgacctga atattcaggg caatggcgct tattccgttc
     9721 tgaagcagtt ggcgacaata gcgttacaaa acggttttat caccgactca caccagttcc
     9781 tgcaaaccct gctgctgcgc gaaaaaatgc actctactgg atttggttcc ggtgtcgcag
     9841 tgccgcacgg taaaagcgcc tgcgttaaac aaccgttcgt attattcgcc cgcaaagcgc
     9901 aggctattga ctggaaagcc agcgatggcg aagacgtcaa ttgctggatc tgcctcggcg
     9961 tgccgcaaag cggcgaagag gatcaggtca aaatcatcgg cacactgtgt cgcaaaatta
    10021 ttcaccagga atttattcat caactgcaac agggcgatac cgaccaggtg ctggccttgt
    10081 taaatcaaac cctcagctca taaggaagtg gcgatggagt catccttacg tattgtcgcg
    10141 atcaccaact gccccgccgg gatcgctcac acctacatgg tggcggaagc cctggaacag
    10201 aaagcccgtt ctctcggcca taccataaaa gtggaaactc aagggtccag tggcgttgaa
    10261 aaccgcttat ccagcgaaga gattgccgct gccgattacg tcattcttgc taccgggcgt
    10321 ggcctgagcg gtgatgatcg cgcgcggttt gccgggaaga aagtttatga gattgccatc
    10381 tccctggcgt tgaaaaatat cgaccagatt ttcagcgaat taccgacaaa ctcgcagctt
    10441 tttgccgcag atagcggcgt gaagcttggc aaacaggaag tgcaaagcgg cagcgtgatg
    10501 agccacctga tggcgggcgt ctctgcggcg ctgccgtttg tcatcggcgg tggtatcctg
    10561 gtggcgcttg ccaacatgct ggtgcagttc ggtttgccgt ataccgatat gtcgaaaggc
    10621 gcgccgtcgt ttacctgggt agttgaatcc atcggttacc ttggcttcac ctttatgatc
    10681 cccatcatgg gcgcttatat tgcctcgtcg attgccgata aaccggcttt cgctccggcg
    10741 tttctggttt gctatctggc gaacgacaaa gcgctgctcg gcacccagtc gggtgcggga
    10801 ttcctcggcg cggtggtgct ggggctggcg attggctatt tcgtcttctg gttccgcaaa
    10861 gtgcgccttg gcaaagcatt acaaccgctg ctcggttcaa tgctgatccc gttcgttact
    10921 ctgctggttt tcggcgtgct cacttactac gttatcgggc cggtgatgtc cgacctcatg
    10981 ggcgggctgc tccacttcct gaataccatt cctccatcaa tgaagtttgc ggcagcgttt
    11041 ctggtcggtg cgatgctggc gttcgatatg ggcggcccca tcaataaaac cgcctggttc
    11101 ttctgcttct cactgctgga aaaacacatt tatgactggt acgccatcgt cggcgttgtc
    11161 gcgttgatgc cgcccgttgc ggcgggtctt gcgacgttca ttgcgcccaa actgtttacc
    11221 cgacaggaaa aagaggctgc cagtagtgcc attgtggtcg gtgccacagt cgcaaccgaa
    11281 ccggctatac cttacgctct ggcagctccg ttgccgatga ttaccgccaa tacgctggcg
    11341 ggcggcatta ccggcgtgct ggtgatcgct ttcggaatca aacgtctggc acctggtttg
    11401 ggtatctttg acccgctgat cggcctgatg tcgccggtag gttcgtttta tctggtgctg
    11461 gcgattggcc tcgcgctgaa catttcattc attatcgtcc tgaaaggatt gtggttacgc
    11521 cgcaaagcga aagccgcgca acaggagctt gtccatgaac attgagttac tgcaacagtt
    11581 gtgcgaagcc agcgccgtta gcggcgatga acaggaagtt cgcgacatcc tgataaacac
    11641 gctggaacct tgcgttaatg aaatcacctt tgatggtctg ggcagctttg ttgcccgtaa
    11701 gggaaataaa ggtccaaaag ttgccgttgt cgggcatatg gatgaagtcg gctttatggt
    11761 cacccacatc gacgagagcg gttttctgcg tttcaccacc attggtggct ggtggaatca
    11821 gtcgatgctc aaccatcggg taaccatacg cacacacaag ggagtgaaaa tccctggtgt
    11881 gattggtttc gtcgcgcctc atgcgttaac ggaaaagcaa aggcaacagc cgctgtcatt
    11941 tgatgagatg ttcattgata ttggcgcaaa cagtcgcgat gaggtggaaa aacgcggtgt
    12001 ggcgatgggc gattttatta gcccggaagc caattttgcc tgctggggcg aagataaagt
    12061 ggtcggcaag gcgctggata accgcatcgg ctgcgcaatg atggctgaac tattgcagac
    12121 ggtgaataat cccgaaatta cgctgtatgg cgtcggcagc gtggaagaag aagttgggct
    12181 acgcggggca caaacctcgg cggaacacat taaaccggat gtagtgatcg tgctggatac
    12241 cgctgtcgca ggcgatgttc cgggcattga taacattaaa tacccgctga aactgggcca
    12301 ggggccgggg ctgatgctgt ttgacaagcg ctacttcccc aaccagaaac tggtagcagc
    12361 gttaaaaaac tgtgccgcac ataacgattt accgctgcaa ttttccacca tgaaaaccgg
    12421 agcgacggat ggcgggcgct acaacgtgat gggcggcggg cgtccggttg ttgcgctgtg
    12481 cctgccaacc cgctatctgc acgctaacag cggcatgatt tcaaaagccg attacgatgc
    12541 tctgctcacg ttgatacggg gttttctgac gaccttaacc gcggagaaag tcaacgcgtt
    12601 tagccagttc cgtcaggtgg attaatgcta aacgaacgcc agttaaagat tgtcgatctg
    12661 ctggagcaac agccgcgcac gcctggtgag ctggcgcaac agactggcgt ttcaggcagg
    12721 accatcctgc gtgatattga ctatctcaac tttaccctta acggcaaagc ccgcattttc
    12781 gccagtggca gtgcgggcta tcagctggaa atcttcgagc gccgcagctt ttttcagttg
    12841 ctgcaaaagc acgataacga cgatcggctg ctggcgctgt tattactgaa tactttcact
    12901 ccccgtgcgc aactcgcctc ggcgcttaat ttgccagaaa cgtgggtagc agagcgtctg
    12961 ccccagttaa aacagcgtta tgaacgcact tgttgcctgg ccagccgccc tggtttgggc
    13021 catttcattg atgagacaga agagaaactc gttatcttgc tggcgaactt gctgcgcaaa
    13081 gatccgtttt taattccgct ggcgggcata acacgagaca accttcagca tttatccacg
    13141 gcctgcgaca accaacaccg ctggccgctc atacagggtg attatctctc cagcctgatt
    13201 ctggcgattt acgccctgcg taatcaactg accgatgagt ggccgcaat
//
LOCUS       DADLTY010000112        13242 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.112, whole genome shotgun sequence.
ACCESSION   DADLTY010000112 DADLTY010000000
VERSION     DADLTY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.112"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..155
                     /locus_tag="JN334_002795"
     CDS             <1..155
                     /locus_tag="JN334_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7438880.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /locus_tag="JN334_002796"
     CDS             384..974
                     /locus_tag="JN334_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7438881.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="JN334_002797"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="JN334_002797"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAY7438882.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="JN334_002798"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="JN334_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="JN334_002799"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="JN334_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HAY7438883.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="JN334_002800"
     CDS             complement(5626..7134)
                     /locus_tag="JN334_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY7438884.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDHSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="JN334_002801"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="JN334_002801"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAY7438885.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /locus_tag="JN334_002802"
     CDS             8609..10255
                     /locus_tag="JN334_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAY7438886.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="JN334_002803"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="JN334_002803"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAY7438887.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="JN334_002804"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="JN334_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HAY7438888.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..>13242)
                     /locus_tag="JN334_002805"
     CDS             complement(12803..>13242)
                     /locus_tag="JN334_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAY7438889.1"
                     /translation="HLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNAL
                     RYCHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLG
                     LAIVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
BASE COUNT         3307 a         3133 c         3451 g         3351 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaatgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cg
//
LOCUS       DADLTY010000113        13102 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.113, whole genome shotgun sequence.
ACCESSION   DADLTY010000113 DADLTY010000000
VERSION     DADLTY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.113"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..1289)
                     /locus_tag="JN334_002806"
     CDS             complement(<1..1289)
                     /locus_tag="JN334_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HAY7438890.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLL"
     gene            complement(1294..1944)
                     /gene="hyfE"
                     /locus_tag="JN334_002807"
     CDS             complement(1294..1944)
                     /gene="hyfE"
                     /locus_tag="JN334_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HAY7438891.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(1956..3395)
                     /locus_tag="JN334_002808"
     CDS             complement(1956..3395)
                     /locus_tag="JN334_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
                     /protein_id="HAY7438892.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIILIVMSLISSVIAATWLQ"
     gene            complement(3412..4359)
                     /gene="hyfC"
                     /locus_tag="JN334_002809"
     CDS             complement(3412..4359)
                     /gene="hyfC"
                     /locus_tag="JN334_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HAY7438893.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(4370..6387)
                     /gene="hyfB"
                     /locus_tag="JN334_002810"
                     /pseudo
     CDS             complement(4370..6387)
                     /gene="hyfB"
                     /locus_tag="JN334_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(6387..7004)
                     /gene="hyfA"
                     /locus_tag="JN334_002811"
     CDS             complement(6387..7004)
                     /gene="hyfA"
                     /locus_tag="JN334_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HAY7438894.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            complement(7257..7727)
                     /gene="bcp"
                     /locus_tag="JN334_002812"
     CDS             complement(7257..7727)
                     /gene="bcp"
                     /locus_tag="JN334_002812"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAY7438895.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(7727..8299)
                     /locus_tag="JN334_002813"
     CDS             complement(7727..8299)
                     /locus_tag="JN334_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HAY7438896.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            8445..9323
                     /gene="dapA"
                     /locus_tag="JN334_002814"
     CDS             8445..9323
                     /gene="dapA"
                     /locus_tag="JN334_002814"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAY7438897.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            9340..10374
                     /gene="bamC"
                     /locus_tag="JN334_002815"
     CDS             9340..10374
                     /gene="bamC"
                     /locus_tag="JN334_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HAY7438898.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            10587..11300
                     /gene="purC"
                     /locus_tag="JN334_002816"
     CDS             10587..11300
                     /gene="purC"
                     /locus_tag="JN334_002816"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAY7438899.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            11468..12331
                     /locus_tag="JN334_002817"
     CDS             11468..12331
                     /locus_tag="JN334_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HAY7438900.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            12346..>13102
                     /locus_tag="JN334_002818"
     CDS             12346..>13102
                     /locus_tag="JN334_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY7438901.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLAS"
BASE COUNT         3406 a         3453 c         3529 g         2714 t
ORIGIN      
        1 agcaataaca gcaggacgat aatcagcagg tgattacgtg ccagtcctgc ggtaacggtc
       61 ataaattcgc taagaaaaat gttgaagggc ggcatccctg ccagcgccag cgccagcgca
      121 ccgccgccaa acagcacggc ggtaaatggc atgattttga gcatcccaca gacgacgttg
      181 agatcgcgcg tgccgtactt gagcagtaca ttgccggaac cgcagaacag cagcgttttt
      241 gccagactgt ggtttaaggt gtgcagcagc gcggcaaaaa ttcccagcgg cccgccaatg
      301 cctagcgcca ccgcgaccag ccccatgttc tccacgctgg agtacgccag cagacgctta
      361 atgtcccgct gtaccagaat gaaaaatgcc gccacggcaa ccgacaacat gccgaagatg
      421 agcaacaacc ggttggggaa atcgctgccg atggcctggc aaataatgat gtaatagcga
      481 atcagcacca acagcgcgca gttcagcaat acggcggaga gcagggcgct gaccggactc
      541 ggcgcttcac tgtgagcatc cggcagccag gcgtgcatgg gaaatagccc ggttttggta
      601 ccaaagccaa ttagcacaaa cacaaaggcc aacagcatta atgttgggtc aagcaaggac
      661 gattgcttaa gaacctcgct ccagaatatc gccatttctg cctgcggcat aacgctggcg
      721 gcgttggcgt ataccagcac ggtaccgaac agaccaaaag cgacaccaac agtacaaata
      781 atgatgtact tccatgctga ttccagcgat gaacgctgac cgtaaatgcc taccagaaac
      841 gccgagctta aggtggtggc ttcgatcgcc gcccacatca caatcaggtt attgctggta
      901 acaaccagca gcatggtgaa caaaaacaga tggaagaaac cgtagtaatc gcacagtgta
      961 acgggtgaaa gctcgccgtg tgccacttca tgacgcatgt agccaatcga gtaaacaccg
     1021 gtgagaaagc caatcacacc aagaatggcg aggaacaaac cgcccagacc atcaatatga
     1081 agccacagtc ccgcagcgaa tatttctcct gcatcagcgg cagtttggac cacccagagt
     1141 gctagaatca gcagcagtgt gatccctaag ctatgtaata cagtcaccgt acaagtcgca
     1201 gagagccccc gtttccggca ggcaaaacag agcagcgaaa aaagcagcgg cgtgagcagg
     1261 agtaaagcga acatcacaga ataactcatc tcattatccc ttcagcgcgg tcaagttgtt
     1321 cacgtccagc gtgccgtggg tacgccatat ttttcttgcc agtaacacca tcacaatgac
     1381 ggcgaagatg gcgtcggtag cgatacctat ttccaccagt tccggtgctc gccaggcaag
     1441 aagcgccagc accagatggg agccgttttc catcaggcag taaccaaaaa tttgccgcag
     1501 gatattgcgt tggctgacaa tgcacagcag gccaagcaga aaatgaccta acgctaccgc
     1561 cagcgccggt ttcagcccgg tagccatcgg tagcttcacg ggctgaacga caaatgcgca
     1621 aagcaggaca attaacgccg cgagcagtgc catcattgcc ggaccgaata acgctttttc
     1681 cgggatgttc tggggaatat ttcgtgcagc gtaagtcatg attaacggta ccagcagcac
     1741 tttggtgata aaggcgctgg cggaccagat cagcagttgc tctgcggcga acaggcacga
     1801 gagagtggcg aaaatagaca ccagcaccag cgactggcag gcgtaaaatc cgcatgacag
     1861 gcgatagctt ttgacgctaa tcacaaacag cgatgtcagc atcatcagtc ccgccagatt
     1921 atttacgatc atagaaccgg tcattcatca tctccttact gcaaccaggt cgcggcgatt
     1981 acgctggaaa tcagcgacat cacaatcagt ataatcaaca ccaggcgcat tgaccctggc
     2041 agcggtgcgg catcggcgac ggcctcgctc ggtttgcccg gcacgacgcg accaaaccag
     2101 cgaataaacc aggcgaaact ggcgaccgat tcaatcatca gcagaatcat ggcgggcagc
     2161 aggatccagt actccactga caacgcaaaa ccggcggcaa acagcgggaa tttactgaag
     2221 aagccgttga acggcggcac accggtaatt gccagcgctg ccacgcagaa acccacgcct
     2281 ggcaacggca gggtgtgcaa tacgccacgc agacgcggca acaagcgcgt gccgcagctg
     2341 taactcagcg cacctgctac aaggaaaaac aggcttttag cgaacgcgtg gttgacgatg
     2401 taggcgatgc taccctccag cgccagccgc gagccgaaga tggagagcga caagccgaag
     2461 aacatccagc caagttgagt gatggtcgac caggccagca accgcttcat atcctgctgt
     2521 ggcaaataca tcagaaagcc ataaagaatg gtgaccagtg tcatgaccat gccaacgccg
     2581 ccaatcacat gcgggatatt gccgccgtcg ataatggcgc gggcaaaaat gtaaacgccc
     2641 actttcacca tcgatgcggc gtggagatag gcgctgatcg gtgttggcgc ttccattgcg
     2701 tccggtagcc acgcttgcat cggtagctgg gccgatttcc cccacgcggc aaacaggatg
     2761 ccgccataaa ccagataacg tgcgtcgcca tgtaactcgc tcatcgcgct aagcacaaac
     2821 gttccggtct gcaaaaacag cgtggcggcg gcaagataca accccaacga accgatatgg
     2881 gtgataagca acgcttttag tgctgaacgc tgcgctttat cgctctggta ataactgatc
     2941 aacgcccagg agcagccgcc cgtaatttca aaaaacaaca actgaccgag cagcgtcgag
     3001 gagagtacca gtcccgccat cgcgccgata aacaccagca aaaatgcgta ataacgattc
     3061 gtgccgttat gcgggtgttc gcgattttta tccgtcagat aacccgtgga gtagatcgtg
     3121 accagcaaac cgagaaacac caccacaaac agaatcagcg tactcacgcg atcaatgacc
     3181 aggccaaata atgccacatc ccccacgttg accaacgtca acgtgacggc aactttatcg
     3241 gcctgataaa acgcggagat cagcgacaac atgcacagcg tggtcagcgc ggcgaacaaa
     3301 accccccatt cggcggcccg acgttgtggc gaaaacgaaa cgaccagtgc gccgataaaa
     3361 ggcagcatta acgtcgtcag agcaagattt tccatattcc gtcagtgctc cttacagacc
     3421 ggttaaccaa aagacccatg caagcgcagc aaggctgaag ccaagccagg tcacatggtg
     3481 aatgagtaaa aaacgcccgc gtgccagcgt gttttctgcg attgaggcca gtacaaaaat
     3541 cagcaaaacc ttgagcagcg taacgacaag tgaagtcagc aggcaggcga gagaaagttc
     3601 ttgcgcgcgc ccaaagggca gaaacagggc cacaaacagt gctgccatca cgacctgttt
     3661 cagccccagc ccccatttcg ctagcgccag cccggcaccg gaatattcgg tcagcgggcc
     3721 ttcctgtaat tcctgttctg cttcagcaac atcaaaggga atttttccca tctcaatgaa
     3781 gcaggcaaaa ccacaggcca gtaacgccag tacggtggtt agcggcgagt tccagcccat
     3841 agccagcgta ttgctgatca tctcgatatg cgtggaacct gctatcagcg ccaataccag
     3901 cagtgagaga ataagcattg gttcgaccag aatgccgagc gtcaactcgc gactggcacc
     3961 gactcccgca aacggacttc cggtatccag cccggaaaga gcaaagaaaa aacgaaacag
     4021 ggcaagaaga tagataaggg tgatcagatc gccgccgccc gcaaaagggg aaacggtaat
     4081 aaatagtggt aaggccatcg ccagcaccag catgctgctg attaataccc acggcatcag
     4141 gcggaacatc agacctgaag atgtcggcgc aacttcctgg cgtttaaaca gtttgtggat
     4201 atcgcgataa tcctgccaga tccccggccc gcggcgggag tgcatacgcg cgcgtatctg
     4261 ccgggaaata cccgtaaaaa gtggggttag catcagcaga atcacggcct gtgctaacgc
     4321 aaaaatgacc agatatccgt cgcaaagagt ttgtctcatg gtgatttcct tagacggcaa
     4381 tagcgattag cagcacaacc agtgcggcga ccacatacag gcaatagaga cgaaagtcgc
     4441 cgctttgcag atgctgtatt tctttgacca gccgttgggt ggcgctcacg atggggcgga
     4501 tcacccgctc atcccagaag ggttctgtgc tctgagccct ggcggtgacg tgcgcaagac
     4561 ctttatttag cctcagcgta gggtcgagct gttgtcgtag acgaaataac gcagagaaga
     4621 ccacacgcag cggctgcatc acgccattgc ctgacggagc catcgcgttt tcccagccgt
     4681 agccgcatgc ccaggcatct cctgtgcgac gaaacgctgc gcggcgcgaa cgacaccaca
     4741 gccagtaaag gcctggcagc aaaggcatcg ccagtagtaa cagcaacagt aatgaaggag
     4801 tgacccgtgt atgaaacgtg ccgggtacaa gtgctattcc gctggcgaca gtgacgggag
     4861 gggtattggt aaacgcatgg gcaatatgca ttatcttcgg tgccagccaa ctggcactaa
     4921 gcgcaattaa tacgcagagt gccgcgagca gtagcatggc gacgatcatc gtatttggca
     4981 cttcctgtgc ctcttcagcg tgtgtactgc gcggcgcacc acagaaagta ataccgtaca
     5041 ttttgacgaa gcacattact gccagcccac cagtgactgc cagcattacc atagcaatag
     5101 gacccgcaag ttgtagcgct acggcttcca cacgacttag tgagaacagc gactgccagg
     5161 tgtaccattc gctgataaaa ccattcagcg gaggaatggc tgatatcgcg aggcaaccaa
     5221 tcaggcatgc tgcggctgtc cacggcatcc gtttcgccag tgcccccatt ttttccatgt
     5281 cgtgggtatg caaacgcgaa ataatcgcgt ccgcgccgag aaatagcagc cctttgaaca
     5341 gcgcatggtt gagcagatga aacagtgcgc cgagcagtcc aaccacggtg agcagcgggt
     5401 cgtgcagtga cagaccgtcc atcgccacac cgactgccag caaaataatg ccgacgtttt
     5461 cgacggtact ccaggccagc agccgtttga tatcctgttc cgccagcgca tatagcacgc
     5521 ccaggagcgc ggagattgcg ccgatcgcca tcaccagaat gccccaccac agaggcaaac
     5581 ccgtttgcgc cagcagatcc atcgctactt tcaggatgcc gaaaataccg attttgacca
     5641 ttacgccaga catcaacgcc gaagcgtgcg atggtgcggc agggtgagcg cgcggcaacc
     5701 agctgtgcaa cggcatcatc ccggctttcg cgccaaaacc gaaaaaggcc agcagaaaca
     5761 ccgccgacgc cagccccgga gaaagtgaaa gcgtgcgaaa ctggcaaaat cgaggctgcc
     5821 gctttcgcgc cccatcagca agaaggcgat cataatcagc accgatccgg cgtgggcgat
     5881 aaaaaagtag agcatgccag cgttgatcga cgttttatcc tgcctggcaa tgaccagaaa
     5941 ccaggaagac agcgacatca tttcaaacag cacgatgaac caaaaagcgt tgtccatcac
     6001 cagcagggca accatcgatg cgatgaaaag attcataaag aagcccatcg ccgccgcgcc
     6061 tttgccctcg tattcgcgca tataagtcaa tgaatagagc gaacaaacca ccaccagcaa
     6121 ggagataact agcaccataa atgccgagag gctatccatt cgcagggtta aatcggcaaa
     6181 gtcaaaaggg gtggcaaaat gggcgactaa agttacacca gcatgtaatt gcgtaattcc
     6241 gctgatgatg ccaatcacgc cgcccaccag cgatgtgatg ccggaaagct taatagccag
     6301 tctgtccaga ccgagtaaaa acagcgaagc cagactggca aacagataga ggatcagcga
     6361 ccaggttaat aattgcaggg catccattag tgttgctcct gagtgaggga aaggggagac
     6421 tccccgccat tggcaaacca gcttgcggca aggcgctgtt tttctttcat ctgacgttgc
     6481 aggctatcgt cggtgatcag ccgtaacgcc tgattcgggc aagcgcgaac gcaggccgga
     6541 ccttctggca agaaatcaca caggtcgcat ttcaccgcga cggtctggac acccggttcc
     6601 cagcgcagca acgcatgttg gggcggcacg ttttcttcgc cgtctttgag tgagccttca
     6661 gcctgaaaaa catattgcgc atgggcattc accggacggc ttcctgaagc gctgattgcg
     6721 ccaaatgggc agaccacggc gcaaagcttg cagccaatac agaggctttc gttgagttgg
     6781 atcgcatcat ccctctgaga gatggcattg accgggcaga cctgcaggca aggggcttcc
     6841 tcacagtgat gacacacgac aggggcagtg attgttgacg tcttcgccag ggccaggcgc
     6901 gggtgttgct gtaaaccttg cgttttatgc acgtccgaac aggcagcgag acaggtatta
     6961 catcctgtac accacagtgg ttcggccacc acaaagcggt tcatgtcggc tccttgaaaa
     7021 tgatatgaaa cattgcattt catattgtat aaaggatgcc attctgatta actcaatcta
     7081 ttcaataagt tatgtattaa ctggattctg aacggtacaa aaatgatggt cattaacgtt
     7141 cagcaaaaaa tggattcgac acccgtgtcg caggggaaag cgagaatgtg atcttgatca
     7201 cgagaagtga ggtatgctgg ccgcaagcgc agccagcacg gaatggagca aagtaatcag
     7261 gcgtgttctt tcagccagtt cagcacaacg tcgtggtgat tgctggtttt gaaatcgtca
     7321 aagacatgtt cgattttgcc atcagcgtca atcaggaagc tgatgcgatg aataccatcg
     7381 taggttttgc ccatgaagga cttttcaccc cagacgccga attgttcgca cacctggtgg
     7441 tcctcatcag acaggagcgt aaagttaagc agctcttttt ccgcaaaacg ggagagtttt
     7501 tcgggtttat cggtgctgat acccagcaca tcaacgcccg cttttttcaa ctcatccatg
     7561 ttatcgcgta agccgcaggc ctgtacggta cagccggggg tcatggcttt cgggtagaaa
     7621 taaaccagaa cacgctgtcc ctggaagtcg gtcaaattaa cttgttctcc gtcttgatcc
     7681 ggcaagctaa atttcggtgc gatatcaccg gctttcagtg gattcattac ttaactccat
     7741 cctgttcatc atgttgggaa taattgacga cgttaatact gccttgtgca ttgagttctg
     7801 tacatagggc tttgaacgct tgctcaatat ttgctgcgtc cgcagatgcg gggctgtggg
     7861 cggttatctg aatatgcaac tgcgcagccc gttcattttc agcaggttgt gtgcgcgaca
     7921 ccagctccgc aatgttcata tgatgcgcgt cgaaaagtgc tgtgaagcgt tcaattaaat
     7981 gcggggagtc tgcaacatcg acctgaaccc agacagatgc tggcattggc ggacgcggac
     8041 gcgccgtcgt gcgcttcatc acgattaaaa gatccagttc ggcacctttc aacggtaacg
     8101 ttgattcaat cagagtgatg gcattccatg aaccggaaag cagcatgata aacgtgaact
     8161 cttctcccag catcgccagg cgactgtctt caatattaca gccgcaacta ctgacatgac
     8221 gggtgatggt gttcacaatt ccagggcgat cggcacccaa cgcagtgatc accagataat
     8281 gttgcgatga cagtgtcaaa ctggttattc ctttaagggg tgagttgttc ttaaggaaag
     8341 cataaaaaaa tcatgcatac aacaatcaga acggttctgt ctgcttgctt ttaatgccat
     8401 accaaacgta ccattgagac acttgtttgc acagaggatg gcccatgttc acgggaagta
     8461 ttgtcgcgat tgttactccg atggatgaaa aaggtaatgt ctgtcgggct agcttgaaaa
     8521 aactgattga ttatcatgtc gccagcggta cttcggcgat cgtttctgtt ggcaccactg
     8581 gcgagtccgc taccttaaat catgacgaac atgctgatgt ggtgatgatg acgctggatc
     8641 tggctgatgg gcgcattccg gtaattgccg ggaccggcgc taacgctact gcggaagcca
     8701 ttagcctgac gcagcgcttc aatgacagtg gtatcgtcgg ctgcctgacg gtaacccctt
     8761 actacaatcg tccgtcgcaa gaaggtttgt atcagcattt caaagccatc gctgagcata
     8821 ctgacctgcc gcaaattctg tataatgtgc cgtcccgtac tggctgcgat ctgctcccgg
     8881 aaacggtggg ccgtctggcg aaagtaaaaa atattatcgg aatcaaagag gcaacaggga
     8941 acttaacgcg tgtaaaccag atcaaagagc tggtttcaga tgattttgtt ctgctgagcg
     9001 gcgatgatgc gagcgcgctg gacttcatgc aattgggcgg tcatggagtt atttccgtta
     9061 cggctaacgt cgcagcgcgt gatatggccc agatgtgcaa actggcagca gaagggcatt
     9121 ttgccgaggc acgcgttatt aatcagcgtc tgatgccatt acacaacaaa ctatttgtcg
     9181 aacccaatcc aatcccggtg aaatgggcat gtaaggaact gggtcttgtg gcgaccgata
     9241 cgctgcgcct gccaatgaca ccaatcaccg acagtggtcg tgagacggtc agagcggcgc
     9301 ttaagcatgc cggtttgctg taaagtttag ggagatttga tggcttactc tgttcaaaag
     9361 tcgcgcctgg caaaggttgc gggtgtttcg cttgttttat tactcgctgc ctgtagttct
     9421 gactcacgct ataagcgtca ggtcagtggt gatgaagcct acctggaagc ggcaccgctt
     9481 gcggagcttc atgccccggc tggaatgatt ttgccggtga cctccggtga ttatgcaatc
     9541 ccggtgacca acggtagtgg tgctgtcggt aaggcgctgg acattcgtcc accagcccag
     9601 ccgttggcac tggtttctgg cgcgcgtacc cagttcacgg gcgataccgc ttcattgctg
     9661 gtggaaaatg gtcgtggcaa tactctgtgg ccgcaggtgg ttagcgtgct gcaggcgaaa
     9721 aactacacca tcacccaacg tgatgatgct ggtcagacac tgaccaccga ttgggtacaa
     9781 tggaaccgtc tggacgaaga cgagcagtat cgtggtcgtt atcaaatctc tgttaagccg
     9841 cagggttatc agcaggcggt tacggttaaa ctgctgaacc tggaacaggc gggcaaaccg
     9901 gttgcagacg cggcttccat gcagcgttac agcacggaga tgatgaacgt tatttccgcc
     9961 ggtctggata aatctgccac tgacgccgcg aacgctgcgc aaaatcgtgc ctccaccact
    10021 atggacgtac aaagtgcagc tgatgacacc ggtttaccaa tgctggtcgt acgcgggccg
    10081 ttcaatgtgg tttggcaacg tctgccagcg gcgctggaaa aagtgggcat gaaagtgacc
    10141 gacagcaccc gttcgcaggg caacatggcc gtaacttata agccgctgtc tgacagcgac
    10201 tggcaggaac tgggcgcgag cgatccaggc ctggcatccg gtgactataa actgcaggtt
    10261 ggcgatttag ataaccgcag cagcctacag ttcatcgatc cgaaaggtca tactctgact
    10321 cagagtcaga acgacgcgct ggtagctgtc ttccaggctg cgtttagcaa gtaaaaatac
    10381 agggctggaa tcatccggcc cttttttctg atatgatacg caaacgtgtg cgtcggcagg
    10441 aaaacgcgat tttagcggta attcgcacga aatttgtttg tcggacgtag ttcggataag
    10501 gcgttcacgc cgcatccgac aaaacatccg gcacaccaga cagcaaaaga ttttaaaacg
    10561 ttaattcaca cccaggagtg ataaagatgc aaaagcaagc tgagttgtat cgtggtaaag
    10621 cgaaaaccgt atacagcacg gaaaacccgg acctgttggt gctcgaattc cgcaatgata
    10681 cgtcagcagg ggatggcgcg cgcattgagc agtttgatcg caaaggtatg gtgaacaaca
    10741 agttcaacta cttcattatg agcaaactgg ctgaagcggg tatcccgact caaatggagc
    10801 gtctgctctc cgataccgaa tgtctggtga aaaagctgga tatggtgccg gttgagtgtg
    10861 tcgtgcgtaa ccgtgctgct ggctctctag tgaaacgtct tggaatcgaa gaaggtattg
    10921 agctgaaccc gccgctgttc gatctgttcc tgaaaaacga tgccatgcac gatccgatgg
    10981 tcaacgaatc ttactgcgaa acctttggct gggtgagcaa agagaacctg gcgcgtatga
    11041 aagagctgac ctacaaagcg aacgacgtgc tgaaaaaact gttcgatgat gctggtctga
    11101 ttctggtcga cttcaagctg gagtttggtc tgtacaaagg tgaagtggta ctgggcgatg
    11161 agttctcccc ggacggcagc cgcctgtggg acaaagaaac actggagaaa atggacaaag
    11221 accgtttccg ccagagcctc ggtggcctga tcgaagccta tgaagccgtc gcccgccgcc
    11281 tgggtgtaca gctggactga tttttctgtt catcatcttg ccgtgcagct ggcacggcaa
    11341 gacaaccgct ctcggaatat atgcattgaa cgctcattcc tccgccattt catcccgctt
    11401 tcctgtggta atcttcctct gaaccaccta cgatcatttc tataatgaat atattgttga
    11461 ggtatctatg cgttggcaag ggcgacgtga aagtgacaat gttgaagaca ggcgcaacag
    11521 ctctggcggt ccgtcaatgg gcggtcccgg tttccgtctg ccgagcggta aaggcgggct
    11581 gattttactg atagtcgtgc tggttgcagg ctactatggc gttgatttaa ccgggttgat
    11641 gaccgggcag ccggtttctc aacaacaatc aacgcggtca attagcccta atgaagacga
    11701 agccgcaaaa ttcacctcgg tgattcaggc aaccacggaa gacacctggg gacaacagtt
    11761 cgaaaagatg ggtaagacct atcagcaacc gaagctggtc atgtaccgtg gaatgacgcg
    11821 caccggctgc ggggcgggcc agtccattat ggggccgttc tattgcccgg cggatggcac
    11881 agtttatatc gatctctcct tctatgatga catgaaagac aaacttggcg cggatggcga
    11941 ttttgcccag gggtacgtta tcgcccatga agtcggtcat catgtgcaga aactgttagg
    12001 catcgagccg aaagttcgtc aactgcaaca aaacgcgacg caggcggaag taaaccgctt
    12061 atctgtgcgt atggaactcc aggccgactg ttttgccggt gtctgggggc atagtatgca
    12121 gcagcaaggc gttctggaaa ctggcgatct ggaagaggcg ctgaatgcgg cgcaggccat
    12181 cggcgatgac cgtttacaac agcaaagtca ggggcgagta gtaccagaca gtttcacaca
    12241 tggcacttct cagcaacgct acagctggtt taaacgtggt ttcgacagcg gcgatccggc
    12301 acaatgcaat acttttggta aaagcattta atttccgggg cagggatggc tgaactgact
    12361 gcgctttaca cattaacagc gcaaatgaaa cgtgaaggga tctgccgctt gctggtgttg
    12421 agcggggaag agggttggtg ttttgatcat gctcttaagt tgcgtgatgc cttacctggc
    12481 gactggctgt ggatttcgcc gcagctagat gctgaaaacc actgttcacc ctcggcgcta
    12541 caaactttac ttgggcgcga gttccggcat gcggtattcg acgcccgcca cggctttgat
    12601 gccgctgcct ttgcagcact tagcggaacg ttgaaagcgg gaagctggct ggttttgtta
    12661 ctccctgtat gggaagagtg ggaaaaccaa cctgatgccg actcgctgcg ctggagtgat
    12721 tgccctgacc ctattgcgac gccgcatttt gtccagcatc tcaaacgcgt acttacggcg
    12781 gataacgacg ctatcctctg gcggcaaaac cagccgttct cgttggcgca ttttactccc
    12841 cgtactgact ggcaccccgc gaccggcgca ccacaaccag aacaacagca actcttacag
    12901 cagctactga ccatgccgcc gggcgtggca gcggtaacgg ctgcgcgtgg gcgcggtaag
    12961 tcggcgttgg cagggcaact catttctcgt attgcgggca gtgcgattgt caccgcgccc
    13021 gcaaaagcgg caacggatgt actggcacaa tttgcgggcg agaagtttcg ctttattgca
    13081 ccggatgcct tgttagccag cg
//
LOCUS       DADLTY010000114        13046 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.114, whole genome shotgun sequence.
ACCESSION   DADLTY010000114 DADLTY010000000
VERSION     DADLTY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.114"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            158..1168
                     /gene="glpX"
                     /locus_tag="JN334_002819"
     CDS             158..1168
                     /gene="glpX"
                     /locus_tag="JN334_002819"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY7438902.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            1265..2011
                     /gene="fpr"
                     /locus_tag="JN334_002820"
     CDS             1265..2011
                     /gene="fpr"
                     /locus_tag="JN334_002820"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HAY7438903.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(2016..2444)
                     /gene="uspD"
                     /locus_tag="JN334_002821"
     CDS             complement(2016..2444)
                     /gene="uspD"
                     /locus_tag="JN334_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HAY7438904.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(2471..2770)
                     /locus_tag="JN334_002822"
     CDS             complement(2471..2770)
                     /locus_tag="JN334_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HAY7438905.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(2982..3422)
                     /locus_tag="JN334_002823"
                     /pseudo
     CDS             complement(2982..3422)
                     /locus_tag="JN334_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            3523..4122
                     /locus_tag="JN334_002824"
     CDS             3523..4122
                     /locus_tag="JN334_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HAY7438906.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            4230..4997
                     /gene="tpiA"
                     /locus_tag="JN334_002825"
     CDS             4230..4997
                     /gene="tpiA"
                     /locus_tag="JN334_002825"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAY7438907.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(5052..5807)
                     /gene="cdh"
                     /locus_tag="JN334_002826"
     CDS             complement(5052..5807)
                     /gene="cdh"
                     /locus_tag="JN334_002826"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HAY7438908.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(5914..6903)
                     /locus_tag="JN334_002827"
     CDS             complement(5914..6903)
                     /locus_tag="JN334_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7438909.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(7223..8185)
                     /gene="pfkA"
                     /locus_tag="JN334_002828"
     CDS             complement(7223..8185)
                     /gene="pfkA"
                     /locus_tag="JN334_002828"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAY7438910.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(8366..9268)
                     /gene="fieF"
                     /locus_tag="JN334_002829"
     CDS             complement(8366..9268)
                     /gene="fieF"
                     /locus_tag="JN334_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HAY7438911.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(9416..9916)
                     /gene="cpxP"
                     /locus_tag="JN334_002830"
     CDS             complement(9416..9916)
                     /gene="cpxP"
                     /locus_tag="JN334_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HAY7438912.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            10066..10764
                     /gene="cpxR"
                     /locus_tag="JN334_002831"
     CDS             10066..10764
                     /gene="cpxR"
                     /locus_tag="JN334_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HAY7438913.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            10761..12134
                     /gene="cpxA"
                     /locus_tag="JN334_002832"
     CDS             10761..12134
                     /gene="cpxA"
                     /locus_tag="JN334_002832"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HAY7438914.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(12240..12914)
                     /gene="yiiM"
                     /locus_tag="JN334_002833"
     CDS             complement(12240..12914)
                     /gene="yiiM"
                     /locus_tag="JN334_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HAY7438915.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT         3296 a         3420 c         3223 g         3107 t
ORIGIN      
        1 agccaatgcc attttgtgta ggcgggataa ggcgttcacg ccgcatccgg catcacgcag
       61 actctgaaac cgtgcggggg caaccccgca cacatcaata atcccaccct tcccctgtgc
      121 tacacttcgc gccattcctt actgcttaga gtttgctatg agacgagaac ttgccatcga
      181 attttcccgc gtcaccgaat ctgcggcgct ggctggctac aaatggttag gacgcggcga
      241 taaaaacacc gcggacggcg cggcggtaaa cgccatgcgt attatgctca accaggtcaa
      301 cattgacggc accatcgtca ttggtgaagg tgaaatcgac gaagcaccga tgctctacat
      361 tggtgaaaaa gtcggtactg gtcgcggcga cgcggtagat attgctgttg atccgattga
      421 aggcacgcgc atgacggcga tgggccaggc taacgcgctg gcggtgctgg cagtaggcga
      481 taaaggctgc ttcctcaatg cgccggatat gtatatggag aagctgattg tcgggccggg
      541 agccaaaggc accattgatc tgaacctgcc gctggcggat aacctgcgca atgtagcggc
      601 ggcgctcggc aaaccgttga gcgaactgac ggtaacgatt ctggctaaac cacgccacga
      661 tgccgttatc gctgaaatgc agcaactcgg cgtacgcgta tttgctattc cggacggcga
      721 cgttgcggcc tcaattctca cctgtatgcc agacagcgaa gttgacgtgc tgtacggtat
      781 tggtggcgcg ccggaaggcg tggtttctgc ggcggtgatc cgcgcattag atggcgacat
      841 gaacggtcgt ctgctggcgc gtcatgacgt caaaggcgac aacgaagaga atcgtcgcat
      901 tggcgagcag gagctggcac gctgcaaagc gatgggcatc gaagccggta aagtattgcg
      961 tctgggcgat atggcgcgca gcgataacgt catcttctct gccaccggta ttaccaaagg
     1021 cgatctgctg gaaggtatta gccgcaaagg caatatcgcg actaccgaaa cgctgctgat
     1081 ccgcggcaag tcacgcacca ttcgccgcat tcagtccatc cactatctgg atcgcaaaga
     1141 cccggaaatg caggtgcaca ttctctgagt gatttgatcg attgagcctt ccagtccttc
     1201 gggactggaa tttttttgtt cggagaacga agataaggca agtcaatcaa aacaggagaa
     1261 aaacatggct gattgggtaa caggcaaagt cactaaagtg cagaactgga ccgacgccct
     1321 gtttagtctc accgttcacg cccccgtgct tccgtttacc gccgggcaat ttaccaagct
     1381 tggccttgaa atcgacggcg aacgcgtcca gcgcgcctac tcctatgtta actcgcccga
     1441 taatcccgat ctggagtttt acctggtcac cgtccccgat ggcaaattaa gcccacgatt
     1501 ggcggcactg aaaccgggcg atgaagtgca ggtggttagc gaagcagctg gcttctttgt
     1561 tctggatgaa gtaccggact gcgaaacgct atggatgctg gcaaccggta cagcgattgg
     1621 cccttattta tcgattctgc aactaggcaa agatttagat cgcttcaaaa atctggtcct
     1681 ggttcacgcc gcacgttatg ccgccgactt aagctatttg ccactgatgc aagaactgga
     1741 aaaacgctac gaaggcaaac tgcgcattca gacggtggtc agtcgggaaa cggcagcggg
     1801 gtcgctcacc ggacggatac cggcattaat tgaaagtggg gaactggaaa gcgcgattgg
     1861 cctgccgatg aataaagaaa ccagccatgt gatgctgtgc ggcaatccac agatggtgcg
     1921 cgatacacaa cagttgctga aagagacccg gcagatgacg aaacatttac gtcgccgacc
     1981 gggccatatg acagcggagc attactggta agcggttact tatcgataaa cggcacgatg
     2041 agcaaatccg cactcatctt attgatcatc ccgcgatatg ccggcatcaa acggttgata
     2101 aatgagtgat gatgaccaca gacaaggagg tcgcactgct ctttttgcat aatttccagc
     2161 agtgtttccg gcatttctcc gcgttcaata cgcagttttg tcttcggcca ttgaatattt
     2221 ttcgtcagtt tatacagctt gttatccgac ttattcttca acaattgaag aatatcttct
     2281 gttgcaggga agtagatacc cggatacaac tcgcttaagc catcatcaat atgaattaac
     2341 gtcaggtgag cgtcattatg tctggcgagc tccagggctt tattcaccag taaggcatct
     2401 tcttcattcc cggaaattgc cacgccaatg tgtttataag ccatgtttaa ctccttctaa
     2461 agcaactcca tcaagctaac gaacgcagtg ataactcaaa aataatcatc tcggcctggc
     2521 atgagaaagt gaaggccgca tcaagctcaa cttgcccctc cagatcctta atcttatagg
     2581 ccacgctggc cccttcatct gcagaggatg cagcggcggc aagctctttc agtttggtca
     2641 taaaagactc tgcttcttcg cgggtggcaa aaaagcgcga aaacttactg gtacagttgt
     2701 cgttatcaat caccgtaccg atatcaatcg cacatccttt agtactgcat ttatctacga
     2761 catctttcat agggcacctc tgtatattcg cctctctgtc gattacgact taagaataat
     2821 gcgccgttaa gggaagaaca aacaagtaat gatcttatgg attaccattt tttttgatat
     2881 tgcttaaggt gaagagttca gaaggatttt ccgggtattg tcatttgcat cgtggaaata
     2941 acaatcccgg aaaaaaatag cagtaagaga tatattaaag gttaattact tgatttattg
     3001 tcggctttat acttcacatc ctgagtatct ttaccatatt tattttcgcc ttgtgtgcca
     3061 acaaacgcac caagatcgat aagcatcatc accagaatca acgtcgggac aaaacgcccc
     3121 accgcccatt gccagacacc cggtaaaatc gctcagttac ccgccagcag catccacgcc
     3181 acaatcatca gaaatgccca tgcgccggaa cgcccgcgat catgcaagcg cttaacagtt
     3241 actgccgctg ttggccagag caagcacaca aggcaaaacg ccgcggtctg aatatcgagt
     3301 agattcttac ccgccagtga gaaaagcacc agcatgcctg cgaaccacag gcctatccaa
     3361 atccagaaat cacggcgtcc aatacgccct ttaaatgaga ataaccattg ctgtatggtc
     3421 atgtaagttc cttgatggtt gtcttttcca ggattctacc cttttgacaa gggcgacaga
     3481 tatcgtttta atcggagcca gtcataacaa aaggtactgt caatgaagcc agggtgtacg
     3541 ctgttttttc tcttatgttc tgcattaacc gttacaacaa cggcgcatgc acaaacacca
     3601 gatacggcaa cgaccgcgcc ttatctgctg gctggagccc ctactttcga tctctccatc
     3661 agccagtttc gagaagactt taacagccag aatcccagcc tgccactgaa cgaatttcgt
     3721 gccatcgaca gcagtcccga caaagccaat ctcactcgtg ctgcaagtaa aattaacgag
     3781 aacttgtatg cttctacagc gctggagcgc ggtaccttaa aaatcaaaag cattcaaatg
     3841 acctggctac ccatccaggg gccagagcaa aaagcagcga aagcgaaagc tcaggaatac
     3901 atggcagcag tgatccgcac actcacccca ttaatgacca aaacacaaag ccagaaaaaa
     3961 ctgcagtcgc tactaacggc ggggaaaaac aaacgttatt acaccgagac agaaggtgca
     4021 ctgcgttatg ttgtcgcgga caacggcgaa aaggggctga ccttcgctgt tgaaccgatt
     4081 aagctggcgc tatctgaatc gcttgaaggt ttgaataaat gacaaaaagc aaagcctttg
     4141 tgccgatgaa tctctatact gtttcacaga cctgctgccc tgcggggcgg ccatcttcct
     4201 ttattcgctt ataagcgtgg agaattaaaa tgcgacatcc tttagtgatg ggtaactgga
     4261 aactgaacgg cagccgccac atggttcacg agctggtttc taacctgcgt aaagagctgg
     4321 caggtgttgc tggctgtgcg gttgcaatcg caccaccgga aatgtacatc gatatggcga
     4381 agcgcgaagc tgaaggcagc cacatcatgc tgggtgcgca aaacgtggac ctgaacctgt
     4441 ccggcgcatt caccggtgaa acttctgccg ctatgctgaa agacatcggc gctcagtaca
     4501 tcatcatcgg ccactctgaa cgtcgtactt accacaaaga gtctgacgaa ctgattgcga
     4561 aaaaattcgc ggtgctgaaa gagcagggtc tgactccggt tctgtgcatc ggtgaaaccg
     4621 aagctgaaaa cgaagcggga aaaactgaag aagtttgcgc acgtcagatc gacgcggtac
     4681 tgaaaactca gggtgctgcg gcattcgaag gtgcggttat cgcttacgaa cctgtatggg
     4741 caatcggtac tggcaaatct gcaactccgg ctcaggcaca ggctgttcac aaattcatcc
     4801 gtgaccacat cgctaaagtt gacgctaaca tcgctgaaca agtgatcatt cagtacggcg
     4861 gctctgtaaa cgcgtctaac gctgcagaac tgtttgctca gccagacatc gacggcgcgc
     4921 tggttggcgg tgcttctctg aaagctgacg ctttcgcagt gatcgttaaa gctgcagaag
     4981 cggctaaaca ggcttaagtc tgacaggtgc cggatttcat atccggcact tactttcctt
     5041 aactcctcgc cttaacgcaa aatctcacac tcatgatcct ggatttcctc ggctgaagca
     5101 cggttcagcg tcagtagatt tcgttgtgtc gccagcaaca caaacgaatt atcactctgc
     5161 cgtaccatcg ccagcccgta gcttcccata tgttcccgcg cctcaggcac ttcttctgca
     5221 agcatcataa atgggctgcg ttgtaccagt tcgctttccg ttacccgacg cgccaggtat
     5281 tcatgcccgc gcaaaccacc tggcagtggc aaccagcggc tgctgatgtt cgccagattt
     5341 ttatccagtt gttcgcgcac atcaggacga atacaagaga tatgaatatg aaaatggttt
     5401 tgcgtatgcc cggtgcggga gttgatcgcc aaagaaaccg cgcgatcggg aaccggctgg
     5461 ccgtattttt tgctcataaa atcacgcgcc tgccaggcca accaaaagaa gttcggcgtt
     5521 gaaggatcgg tcaacaaagg actttcagta ccgttaatac gatacgttgg catcaacaga
     5581 tattgcagtg gaccattaag atcttttaaa accacgtatc cggcattggg tttgacttcc
     5641 gcacatggcg aaggattttg attttgctgc tgattgggca aacattcctc aaggacaatc
     5701 ttacgtaatg tatccgactc ttcaccggtt aatttccagt aaccaatacc ggcagccaca
     5761 acggcgataa ctatcatcac caaaaaaaga agacccgctt ttttcatctt tttttccctg
     5821 tacctcaaag agatgcaagg gtaacgcaaa atcgtgacaa ataaaaaacc cggtcgcgag
     5881 aaaccaccgg gtttgataat tatcctaggg aaatcagcgt ttgctgatct gatcgaacgt
     5941 accgccgtta gcaaaatgct ctttttgcgc tttcgtccag ccgccgaact cttcatcaat
     6001 ggtgaataac ttcagctttg gaaacgcatt ttcgtacttt ttcgctactt cagcgtcgcg
     6061 cggacggtag tagtttttcg cggcaatttc ctgaccttct ggcgagtaga gatatttcag
     6121 gtaggcttcc gccacctctt tggtgccttt tttctcgacc actttatcga ccaccgacac
     6181 cgttggctct gcgaggatag actcactcgg cgtgacgatt tcgaatttat ctttccccag
     6241 ttcattcgct gccagcagag cttcgttttc ccaggcgatc agtacatcgc caatcccgcg
     6301 ctcgacaaag gtgttagtgg agccgcgcgc gccagaatcc agaacttcga cgtttttata
     6361 cagtgcccga acaaaatcct gtgcttttgc ctgatcgttg ttgttgtgat gcagcgcgta
     6421 gccccaggca gccaggtagt tccagcgtgc gccaccagag cttttcggat taggcgtgat
     6481 caccgaaaca cccggtttaa tcagatcgtt ccagtcatgg atctgcttcg gattgccctt
     6541 acgcaccagg aaaacaatgg tggaagtgta cggtgcggag ttatccggca gacgtttgat
     6601 ccactcttta tcaatccgcc cgcgttccgc aatcgcgtcc acgtcatagg ccagagccag
     6661 cgtgacaaca tcagcttcaa taccgttgat taccgacgtc gcttgtttac ctgaaccacc
     6721 gtgtgactga cgaatcacca cgttatcacc agtttgctgt ttccagtggg cgctgaatgc
     6781 cttgttgtac tgttcgtaca attcgcgcgt tggatcatat gaaacgttaa gaagctgaat
     6841 atcctttgcc ataacgctgg ttgccgccag caaaaatgtt aaccctacgc cccacttgtt
     6901 catcgcccga ctctcttatg ttgtgttgtg atgagcaaag cgtgccagaa ggttaaccaa
     6961 acattaaaga ataaaaaaag attggctata acttgcgggt atatgttgag ggattaaaaa
     7021 ggcggaagaa atccgcctca tattgctgac aaagtgcgct ttgtccatgc cggatgcggc
     7081 gcgaacgcct tatccggcct acaaaagttt gcaaattcaa taaattgcag aattcatgta
     7141 ggcctgataa gcgaagcgca tcaggcattt ttgcttctgt catcggtttc aggctaaagg
     7201 aatctgcctt tttccgaaat cattaataca gttttttcgc gcagtccagc cagtcacctt
     7261 tgaacggacg cttcatgttt tcgatagcgt cgatgatgtc gtggtgaacc agctgttcgt
     7321 tctggatacc tacacaacga ccgccgtaac ctgccagcag cagatcgata gcgtaagcgc
     7381 ccatacggga agccagaata cggtcgtaag gcaccggaga accaccgcgc tggatgtggc
     7441 ccagcacagt tgcgcgggtt tcacgaccgg tttctttctc gatgaaatgc gccagttcgt
     7501 caacatcaca catatgttcg gtaatcgcca cgatcgcgtg ttttttacct ttcgcgatac
     7561 ccgctttgat ttcgtttacc aggtcttcac ggctgaattc aacttccgga actacaacga
     7621 attcacagcc accggcaatg gccgcagcca gggtcagatc gccacaataa cggcccatca
     7681 cttccaccac ggaaatacgc tggtgagaag aagaggtgtc acgcagacgg tcgatcgctt
     7741 ctacaacggt gctcagcgca gtgaagaaac cgatagtgta gtcagtgcct ttgatgtcgt
     7801 tgtcgatagt gcccggcaga ccgatgcacg ggaagcccat ttcggtcaga cgcattgcac
     7861 ccatgtagga accgtcaccg ccgataacca ccagcgcgtc gataccacgt tttttcaggt
     7921 tttcgatagc cacggcgcgg atgttctcgt cgcggaattc cgggaaacgc gcagaaccga
     7981 ggaacgtacc gccacggttg atcatgtcag acacgctgta acggtctagc tgtaccatac
     8041 ggtcttcata cagacccaga tagccgtcat aaatacccat tacttccaga ccttctgtca
     8101 gcgcagaacg aacaaccccg cgaattgcgg cgttcatgcc tggcgcatca ccgccgcttg
     8161 tcaacacacc tattttctta atcatgacta cctctgaact ttggaatgca aaatgaaatc
     8221 tgttgccgga agtcttcttg cacatcgaag tgatccaacg aatgtgcaaa tagtataaca
     8281 atcacttcct gctgaattga ttcaggtcag gccaaatggc ggtattttat acacaaaatg
     8341 cgggtctggc tctcttttat actgattatg aaagcataga ccgtttaccc tccctgggta
     8401 cgacggaaca ggggtcctga tggataatca catccgatcc cggaaaacgc cgtaaaatag
     8461 cctgctctac ctgatccgcc accatatgtg cctgaaccaa aggcagagag tcttccattt
     8521 ccaaatgaat ctgaataaag cgggtcggcc ctgactgccg cgtgcgaaga tcgtgagcgc
     8581 cgctaacacc cggccaggaa gtcacgatat caataatttc ttgccgttct tcatcaggca
     8641 atgcgcgatc cagtaatgac tgtaccgcct catatcccat gcgtaacgcg ctatataaaa
     8701 tatagatgcc gattcccaat gcaaacagag catcggcgcg atgccagccg taccaagaca
     8761 accccagcgc cagcagaatt gcgccgttca tcataacatc agactggtaa tgtagcatat
     8821 cagcccgcac cgcctggctt tgcgtccggc gcaccaccca ccgctgaaac gagacaagga
     8881 taatcgtaca aattagcgcc acaattgtca cgataacccc gacgcctgga tctgtcatcg
     8941 gtgttggaga taccagatgt tgaatacccg tcaaaaacag gaatagtgcc gaaccggaga
     9001 taaacatact ttgcgccagc gccgcgaggg actcagcttt accgtgacca aacgagtgat
     9061 tatcgtcggc aggttgcagg gaatatcgca ccaccagtaa attcgtcaac gacgcgccga
     9121 tatccaccag cgaatccacc agcgcggcga gaatactcac cgacccggta taccaccatg
     9181 caaaaatttt aatcagcaat agcaacgaag ccatcgccgt cgcagcaatc gccgcccgac
     9241 tgaccagccg tccataagat tgattcataa atactcccgc tatcaactga cgctagtata
     9301 acggaagcaa atcatctgca atgcattaag cagcaggcaa attgaggata aaaaaaaccc
     9361 cacatcatgt gggggaagac agggatggtg tctatggcaa ggaaaacagg gtttactact
     9421 gggaacgtga gttgctacta ctcaatagct tcaacgatga acttttttgc cattgcgtca
     9481 cgtcacgcaa ctgctccatt cgttgttgat gtttctcgtt taaaaccgct tgctgctccg
     9541 gcgttaacag gcgatacatt tggttgcgga cttttgccat ctcaacctga cgagcaattt
     9601 gctcattcgc cattttttct gcctgtgcgc gcacagcgtt ttcatcaaaa ttttctgcgg
     9661 tgacaaggcg atgcattgtc tccagttcgc taacattaac aggaggctgt tcgtgccggg
     9721 cctgttgcat aagatctcgc atctgctgac gctgatgttc ggttaaactt atgccgtcga
     9781 acatatggct ctgcgtactg cgctgcgtaa gttcttcacc cggatgccag ttatcgcctg
     9841 aaccgacttc agcagcgtgg cttaatgaac tgactgccag cgttgaggcc atgacggcag
     9901 cggtaactat gcgcatcatt tgctcccaaa atctttctgt cgcgattcaa cgatagagag
     9961 tttacgattc aggctgcaaa catgcgtcag ggggtgtaaa acaacgtaaa gtcatggatt
    10021 agcgacgtct gatgacgtaa tttctgcctc ggaggtattt aaacaatgaa taaaatcctg
    10081 ttagttgatg atgaccgaga gctgacttcc ctattaaagg agctgctcga gatggaaggc
    10141 ttcaacgtga ttgttgccca cgatggggaa caggcgcttg atcttctgga cgacagcatt
    10201 gatttacttt tgcttgacgt aatgatgccg aagaaaaatg gtatcgacac attaaaagca
    10261 cttcgccaga cacaccagac gcctgtcatt atgttgacgg cgcgcggcag tgaacttgat
    10321 cgcgttctcg gccttgagct gggcgcagat gactatctcc cgaaaccgtt taatgatcgt
    10381 gagctggtgg cacgtattcg cgcaatcctg cgccgttcgc actggagcga gcaacagcaa
    10441 aacaacgaca acggttcacc gacactggaa gttgatgcct tagtgctgaa tccaggccgt
    10501 caggaagcca gcttcgacgg gcaaacgctg gagttaaccg gtactgagtt taccctgctc
    10561 tatttgctgg cacagcatct gggtcaggtg gtttcccgtg aacatttaag ccaggaagtg
    10621 ttgggcaaac gcctgacgcc tttcgaccgc gctattgata tgcacatttc caacctgcgt
    10681 cgtaaactgc cggatcgtaa agatggtcac ccgtggttta aaaccttgcg tggtcgcggc
    10741 tatctgatgg tttctgcttc atgataggca gcttaaccgc gcgcatcttc gccatcttct
    10801 ggctgacgct ggtgctggtg ttgatgttgg ttttgatgtt acccaagctc gattcacgcc
    10861 agatgaccga gcttctggat agcgaacagc gtcaggggct gatgattgag cagcatgtcg
    10921 aagcggagct ggcgaacgat ccgcccaacg atttaatgtg gtggcggcgt ctgttccggg
    10981 cgattgataa gtgggcaccg ccaggacagc gtttgttatt ggtgaccacc gaaggccgcg
    11041 tgatcggcgc tgaacgcagc gaaatgcaga tcattcgtaa ctttattggt caggccgata
    11101 acgccgatca tccgcagaag aaaaagtatg gccgcgtgga actggtcggt ccgttctccg
    11161 tgcgtgatgg cgaagataat taccaacttt atctgattcg tccggccagt agttctcaat
    11221 ccgatttcat taacttactg tttgaccgcc cgctattact gctgattgtc accatgttgg
    11281 tcagtacgcc gctgctgttg tggttggcct ggagtctggc aaaaccggcg cgtaagctga
    11341 aaaacgctgc cgatgaagtt gcccagggaa acttacgcca gcacccggaa ctggaagcgg
    11401 ggccacagga attccttgcc gcaggtgcca gttttaacca gatggtcacc gcgctggagc
    11461 gcatgatgac ctctcagcag cgtctgcttt ctgatatctc tcacgagctg cgcaccccgc
    11521 tgacgcgtct gcaactgggt acggcgttac tgcgccgtcg tagcggtgaa agcaaggaac
    11581 tcgagcgtat tgaaaccgaa gcgcaacgtc tggacagcat gatcaacgat ctgttggtga
    11641 tgtcacgtaa tcagcaaaaa aacgcgctgg ttagcgaaac catcaaagcc aaccagttgt
    11701 ggagtgaagt gctggataac gcggcgttcg aagccgagca aatgggcaag tcgttgacag
    11761 ttaacttccc gcctgggccg tggccgctgt acggcaatcc gaacgccctg gaaagtgcgc
    11821 tggaaaacat tgttcgtaat gctctgcgtt attcccatac gaagattgaa gtgggctttg
    11881 cggtagataa agacggtatc accattacgg tggacgacga tggtcctggc gttagcccgg
    11941 aagatcgcga acagattttc cgtccgttct atcggaccga tgaagcacgc gatcgtgaat
    12001 ctggcggtac aggattgggg ctggcgattg ttgaaaccgc cattcagcag catcgtggct
    12061 gggtgaaggc agaagacagc ccgctgggcg gtttacggct ggtgatttgg ttgccgctgt
    12121 ataagcggag ttaaactccg catttgtagg caggataagg cgtttacgcc gcatccggca
    12181 tttgagcagg atgcctgatg cgacgctgat agcgtcttat caggcctaca ctccgggggt
    12241 tattttcccc acaaccggcg ggaaaaatct tcgatcttgc cgctcagtcg gcgcttttgc
    12301 atcgtccgcg tccagctttt cgataacccg gcggcggaga gtaaacggtg atactgatcg
    12361 tcatcaaacg gcatatgcca tgcgatggcg gcagcctcct gcacggtcac atcgctgaca
    12421 cgggaaacca actccagcgg cgcgtccgca gacactttcc ctggcgctat cacgctatac
    12481 aaccacccca ccttacctgt gttttgcatc aactgcgcta tatcgctgat atcaaaatgg
    12541 taattgagtt tatagcaagg cgagcgcggc tggctgacct gaattaatgc ctctccccag
    12601 cggaaaatat cgcccatatg aacattactt tccgtgagac cgtcggttga gagattttcg
    12661 ccaaacgcag gcgcaacaaa caactccgcc tgttccggaa attcccgcac ccagtagaga
    12721 tagtgctcac gaggataatg acacagcgct ctgtctggcc cgccgtgaac ctttttctcc
    12781 gcctgctcgt caccttccag ccccagctcc gtcagcatca actcaccatc aacctggatt
    12841 ttggcaattg cgctgggttt gccttcggga taagcctgaa tcttgcctgt gtatacatca
    12901 accggatatc gcatctgttc cctctccacg agaaatttcc ccattagacc atatttattt
    12961 tatgtcagcc gtgctgtttt cgcggtaagt atccaggcag gaactcaata gcaaaccgga
    13021 cgaacgttag ttcatccggc tgtggg
//
LOCUS       DADLTY010000115        12906 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.115, whole genome shotgun sequence.
ACCESSION   DADLTY010000115 DADLTY010000000
VERSION     DADLTY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.115"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(128..1579)
                     /locus_tag="JN334_002834"
     CDS             complement(128..1579)
                     /locus_tag="JN334_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAY7438916.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="JN334_002835"
     CDS             complement(1806..3224)
                     /locus_tag="JN334_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY7438917.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="JN334_002836"
     CDS             complement(3378..3722)
                     /locus_tag="JN334_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HAY7438918.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="JN334_002837"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="JN334_002837"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HAY7438919.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5647)
                     /locus_tag="JN334_002838"
     CDS             complement(4712..5647)
                     /locus_tag="JN334_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY7438920.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="JN334_002839"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="JN334_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY7438921.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="JN334_002840"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="JN334_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HAY7438922.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="JN334_002841"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="JN334_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HAY7438923.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="JN334_002842"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="JN334_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HAY7438924.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="JN334_002843"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="JN334_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HAY7438925.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="JN334_002844"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="JN334_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HAY7438926.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="JN334_002845"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="JN334_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HAY7438927.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11365..12483
                     /gene="ydeE"
                     /locus_tag="JN334_002846"
     CDS             11365..12483
                     /gene="ydeE"
                     /locus_tag="JN334_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HAY7438928.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGLRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="JN334_002847"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="JN334_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HAY7438929.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3400 a         3299 c         2948 g         3259 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc ctccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaat
//
LOCUS       DADLTY010000116        12660 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.116, whole genome shotgun sequence.
ACCESSION   DADLTY010000116 DADLTY010000000
VERSION     DADLTY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.116"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            126..1823
                     /locus_tag="JN334_002848"
     CDS             126..1823
                     /locus_tag="JN334_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAY7438930.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1957..2967
                     /gene="adhP"
                     /locus_tag="JN334_002849"
     CDS             1957..2967
                     /gene="adhP"
                     /locus_tag="JN334_002849"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HAY7438931.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3113..3397
                     /locus_tag="JN334_002850"
     CDS             3113..3397
                     /locus_tag="JN334_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY7438932.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3625..4278)
                     /gene="fdnI"
                     /locus_tag="JN334_002851"
     CDS             complement(3625..4278)
                     /gene="fdnI"
                     /locus_tag="JN334_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HAY7438933.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4271..5155)
                     /gene="fdxH"
                     /locus_tag="JN334_002852"
     CDS             complement(4271..5155)
                     /gene="fdxH"
                     /locus_tag="JN334_002852"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY7438934.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5168..8215)
                     /gene="fdnG"
                     /locus_tag="JN334_002853"
     CDS             complement(5168..8215)
                     /gene="fdnG"
                     /locus_tag="JN334_002853"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7628..7630),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY7438935.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8447..9328
                     /gene="yddG"
                     /locus_tag="JN334_002854"
     CDS             8447..9328
                     /gene="yddG"
                     /locus_tag="JN334_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HAY7438936.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9583..10683
                     /gene="ompC"
                     /locus_tag="JN334_002855"
     CDS             9583..10683
                     /gene="ompC"
                     /locus_tag="JN334_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY7438937.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10847..11332
                     /locus_tag="JN334_002856"
     CDS             10847..11332
                     /locus_tag="JN334_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY7438938.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11442..12431
                     /locus_tag="JN334_002857"
     CDS             11442..12431
                     /locus_tag="JN334_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY7438939.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3340 a         3127 c         2941 g         3252 t
ORIGIN      
        1 gattaaacac cattgcctgc gcaatggtgt ttttatctgc tttatacttg gggacgacgc
       61 cctggcggta aagcaaagac gataaaagcg tgccagggat ggatattcaa aaaagagtga
      121 gtgacatgga accaaaaaca aaaaaacagc gttcgcttta tatcccttac gctggccctg
      181 tactgctgga atttccgttg ttgaataaag gcagtgcctt cagcatggaa gaacgccgta
      241 acttcaacct gctggggtta ctgccggaag tggtcgaaac catcgaagaa caagcggaac
      301 gagcatggat ccagtatcag ggattcaaaa ccgaaatcga caaacacatc tacctgcgta
      361 acatccagga caccaacgaa accctcttct accgtctggt aaacaatcat cttgatgaga
      421 tgatgcctgt tatttatacc ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct
      481 accgccgttc acgcggcgtg tttatctctt accagaaccg ccacaatatg gacgatattc
      541 tgcaaaacgt gccgaaccat aatattaaag tgattgtggt gactgacggt gaacgtattc
      601 tggggcttgg tgaccagggc atcggcggga tgggcattcc gatcggtaaa ctgtcgctct
      661 ataccgcctg tggcggcatc agcccggcgt atacccttcc ggtggtgctg gatgtcggaa
      721 cgaacaacca acagctgctt aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca
      781 ctgacgatga atactatgaa ttcgttgatg aatttatcca ggctgtgaaa caacgctggc
      841 cggacgtgct gttgcagttt gaagactttg ctcaaaaaaa tgcgatgccg ttacttaacc
      901 gctatcgcaa tgaaatttgt tcttttaacg atgacattca gggcactgcg gcggtaacag
      961 tcggcacact gatcgcagcc agccgcgcgg caggtggtca gttaagcgag aaaaaaatcg
     1021 tcttccttgg cgcaggttca gcgggatgcg gcattgccga aatgatcatc gcccagaccc
     1081 agcgcgaagg attaagcgag gaagcagcgc ggcagaaagt ctttatggtc gatcgctttg
     1141 gcctgctgac ggacaagatg ccgaacctgc tgcctttcca gaccaaactg gtgcagaagc
     1201 gcgaaaacct cagtgactgg gataccgaca gcgatgtgtt gtcactgctg gatgtggtgc
     1261 gcaatgtaaa accagatatt ctgattggcg tctcaggaca gaccgggctg tttacggaag
     1321 agatcatccg tgagatgcat aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc
     1381 cgacgtctcg cgtggaagcc acaccgcagg acattatcgc ctggaccgaa ggtaacgcgc
     1441 tggtcgccac tggcagcccg tttaatcctg tggtatggaa agataaaatc taccctatcg
     1501 cccagtgtaa caacgccttt attttcccgg gcatcggcct gggtgttatt gcttccggcg
     1561 cgtcacgtat caccgatgag atgctgatgt cggcaagtga aacgcttgct cagtattcgc
     1621 cgctggtcct gaacggcgaa ggtctggtac taccggaact gaaagatatt cagaaagtct
     1681 cccgcgcaat tgcgtttgcg gttggcaaaa tggcgcagca gcaaggcgtg gcggtcaaaa
     1741 cgtctgctga agctttgcaa caagccattg acgataattt ctggcaagcc gaataccgcg
     1801 actaccgccg tacctccatc taagcctgca cccggtagtg aaggctaccg ggctatttcc
     1861 ctctcccttt ttcagatctc atccatactg ggtagtggcg aataaatctc atttgcctca
     1921 cctgctatgc agaacatcat ccgaaaagga ggaactatga agactgcagt tgttacgaag
     1981 gatcatcatg ttgacgttac ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg
     2041 ctgaaaatgg agtgttgtgg tgtatgtcat accgatcttc atgttaagaa tggcgatttt
     2101 ggtgacaaaa ccggcgtaat tctgggccat gaaggcatcg gtgtggtggc agaagttggc
     2161 ccaggtgtca cctcattaaa accaggcgat cgtgccagcg tggcgtggtt ttacgaagga
     2221 tgcggtcatt gcgaatactg taacagtggt aacgaaacgc tctgccgttc agttaaaaat
     2281 gccggataca gcgttgatgg cgggatggag gaagagtgca tcgtggtcgc cgattacgcg
     2341 gtaaaagtgc cagatggtct ggactcggcg gcgcccagca gcattacctg tgcgggggtc
     2401 accacctaca aagccgttaa actgtcaaaa attcgtcccg gtcagtggat tgctatctac
     2461 ggtcttggcg gtctgggtaa cctcgccctg caatacgcaa agaatgtctt taacgccaaa
     2521 gtgatcgcca ttgatgtcaa tgatgagcag ttaaaactgg caaccgaaat ggatgcagat
     2581 ttagcgatta actcacgcac cgaagacgcc gccaaaattg tgcaggagaa aaccggtggc
     2641 gctcacgctg cggtggtaac agcagtagct aaagctgcgt ttaactcggc agttgatgct
     2701 gtccgtgcag gcggtcgtgt tgtggctgtc ggtctgccgc cggagtctat gagcctggat
     2761 atcccacgtc ttgtgctgga tggcattgaa gtggtcggtt cgctggtcgg cacgcgccag
     2821 gatctaaccg aagccttcca gtttgccgcc gaaggcaaag tggtgccgaa agtcgccctg
     2881 cgtccgttag cggacatcaa caccatcttt accgagatgg aagaaggcaa aatccgtggc
     2941 cgtatggtga ttgatttccg tcactaagag gtctttgctg cgactcccat gttcgggtcg
     3001 cagcatcgca cactctccaa catgaaatgg ctaaatggat tattgttgcg taggaaggaa
     3061 caataatgat agtgttccca gagaagtaac gctactgaga gtaaagacac taatgaaaat
     3121 ggccaatcat ccccgcccgg gggacattat tcaggaatca ctggacgaac ttaatgtcag
     3181 cctgcgcgag tttgccagag caatggaaat tgcgccctca acggcaagcc gattgctgac
     3241 cggaaaagca gctttgacgc cagaaatggc aattaaactt tccgtggtga tcggcagttc
     3301 gccgcaaatg tggctgaatc tgcaaaacgc ctggagtctg gcagaggcag aaaaaacggt
     3361 ggttgtgtcg cgattacgcc gtctggttac gcaataaaaa atggcaccct ataaagcgcc
     3421 atttgaaact gatgacaaac gcaacattgc ctgatgcgct acgcttatca ggcctacacg
     3481 ttcacttcaa tctattgtat ttgaagggtt ttcatgccgg ataaggcatt cacgacgcat
     3541 ccgggatgaa caaagcccac gttgtctaca atatgaaaat ggcgcctttt acagcgccat
     3601 tttcagctta aagttcaaaa gagattatat cccttcttca ctctcttttt tcgcttctgc
     3661 cttctcgatt tcacgatacc agcgcggatg gtgtttcttc gcccagcgac ggctcacctt
     3721 cccttcgatc atccctttaa tcgacccttt cacccagaat gccatataca tatggatcag
     3781 gatggcgtgg atcaggatga tacccgcagc cgcgtggatc agcaggctgt agcgaacaac
     3841 ctgcatcggg aagtactgcg caaagtacgg acgccagata atcaccccgg tcaccagcag
     3901 cacgaaaatc atgctcatga tcgaccagaa catcatcttt tgcccggcgt tgtacttacc
     3961 aacatccgcc actttgtgct cattgccttt taatacttcg acaatgttca acagccacgg
     4021 aatatctttc ttatccggga tgttgtgatg cacaaaacgc acaaacataa acatcagtgc
     4081 gacgaaaatc gcaatgccga agaaggggtg caaaatgcgt cccatctgcg gcgtaccgaa
     4141 ggtttgcgtc agccattgca gcgtcgggaa gaagaacgaa atcccggaca gcgccaccag
     4201 gaagaagcaa atcaccacgg tccagtgaca ggcgcgatca ataaatttgg tgcgcacaat
     4261 cattttcgac ttactcatga tgatcctcct cgtcatcgtc cacttcctta ttcgggccaa
     4321 taccgatgta gtggaaaatc aacccggcaa aagtggcaat aaagccagcc gctgccagcg
     4381 gtttcaacgc gcctttccac aggcttaccg aggtgtcgat cttcggatct ttcggcagac
     4441 cgtgatacag ctccggctga tcggcgtgat gcagcacgta cataacgtgc gtaccaccga
     4501 ccccttccgg gttgtagacg ccagcatgtt cgtaaccacg cgctttcagt ttcgccacgc
     4561 gctgttccgc cagctccagc atctccttct tggtgccgaa gtggatagcc ccggtcggac
     4621 aggttttcac acaagccggt tcctggccga cgctgacgcg atcgacgcag agcgtgcatt
     4681 tatatacccg gttatcctct ttgttgaggc gcggaatatt aaacggacac ccggcaatgc
     4741 agtaaccaca gccgatgcag ttttccgact ggaaatcgac aatcccgtta gcgtactgaa
     4801 tgattgcacc agcagacggg cacgccttca ggcagcccgg atcttcacag tgcatacagc
     4861 cgtctttacg gatcagccac tccagcttgc cattctgttc ggtttcgcta aagcgcatca
     4921 ccgtccagga cttggcgctc agatcggcgg ggttatcgta aaccccgacg cagtgcccca
     4981 cttcatcacg gatgtcgttc cactccgaac acgccacctg acaggcttta cagccgatac
     5041 aggtggaaac gtcgataagt ttcgcgactt ctgctttgta atcacgcacc tgagaaggcg
     5101 gcgtgatgga gttagttgcg gaccttttga taatatcctg cgtttccata gccatctgtt
     5161 cgccccctta cgccttctcg atgttgacta agaacgcttt atattccggc gtttgcgagt
     5221 ttgcatcacc gacattcggc gtcagagtgt tagcgatata acctttacgc gcgacaccct
     5281 caaagcccca gtggattgga atacccaccg tttcaacctg ctgaccgttt acattcagcg
     5341 gtttcagacg gcgcgttacc actgccaccg cgcggataaa gcctcgcttg ctggagacag
     5401 tgacacgatc gccattatta atgcctttcg ccgccgcctg cgtttcgctg atttccacaa
     5461 actgttccgg ctgagcaatt gcgttgagca acgcgtgctt ggtccaggtg tggaagtgct
     5521 cggtcagacg ataggtcgta cccacatacg ggaactgctc ttttttaccc atccgcagcg
     5581 cgtcttgttc atacagacga acaaccgggt tagacaccac gttcggatgc agcgggttag
     5641 tgcccagcgg cgtttcaatc ggctcgtagt gttccgggaa cggaccttcc gccattttgt
     5701 tgatggcaaa caggcgtccc atcccttccg gctgcataat aaacggcccg gttggcgtac
     5761 ccggtgcggc attgccgaag tccggaatat cgttacccgt ccacttgctg ccgttccact
     5821 ggatcagcat ccgtttcgga tcccacggtt taccgttgat atccgccgaa gcacggttgt
     5881 agagtacgcg acggttgagc ggccacgccc aggcccatcc cagcgtattc cccagaccgg
     5941 acgggtcgga gttatcgcgg tttgccatct ggttaccctg ctctgtccag ctaccggtgt
     6001 agatccagca agaagatgcg gttgtaccgt catcacgcag atgcgcaaag ctactcagca
     6061 actgaccttt cttcgcaatc agcacgccat tagcatcata gagatcttcc aacgcatagc
     6121 cgttgttctc tttagctact tcgtcagatt gcggttcgtg cggctgcttg tagttccagc
     6181 tcatcttcat cagcggttct acgcctttac caccttcggc ctggtacaac tcgcgcagat
     6241 gatggtagat acccgccaga atttcgccgt cgttacgcgc ttcgcccggc gcgtcctgac
     6301 ctttccagtg ccactgcaac cagcgaccgg agttagcgat agaaccatcc tcttcagcaa
     6361 agcaggtcga aggcagacgg aatacttcag tctgaataga cgccggatcg acatcgttcg
     6421 actcgccgtg gttctgccag aaggtagagg tttcagtcac cagcggatcg ataaccacca
     6481 tgtacttcag tttgctcagg cagctcacca ctttgttttt atccgggaag gacgcaaccg
     6541 ggttaaagcc ctggcagaaa taaccggtaa ctttgccttc atccatcatg ttgaaatact
     6601 tgatgacgtc gtaggtctgg tcccacttcg gcagccagtc atagccccag ttgttctctt
     6661 tctgcgcggc atcgccatag aaagatttca tcaggctaac gaagaacttc ggatagttgc
     6721 tccagtagtt cacctgatca gccagcgtcg ctttcggcgt gttcgcttcc agatacgact
     6781 gcaaatcaac ctgtttttct gacggcagcg tcagataacc tggcaggctg gtagagagca
     6841 gacctaagtc agtcagcccc tgaatgttgg agtgaccacg caatgcgttc acgccgccac
     6901 cggccatacc catgttaccg agcagcaact ggatcatcgc catagtacgg atgttctgcg
     6961 cacccacggt gtgctgcgtc cagcccagcg cgtacaggaa ggtggttgtg cgatccggtg
     7021 cgctggtgga ggccagcact tcacacactt tcaggaagtc ggcttttggc gtaccacaga
     7081 tgttttcaac aacgtccggc gtgtagcggg aaacgtgcgc tttcagcagg ttccacacac
     7141 agcgcggatg agtcagtgtt tcatcgcgtt tcgcatagcc gttttcatcg aactgatagt
     7201 tccaggacga tttgtcgtac tggcgttttt cagcgtcata accgctgaac aggccatctt
     7261 cgaaagcaaa atcatcacgc accagtaggc tggcgttggt gtaatgttta acgtattcgg
     7321 cgttgatttt gttgttttcg atcaggtagc gcaaaatgcc agacaggaac gtaatgtccg
     7381 taccggaacg aataggtgcg taaatatccg ccacagaagc ggtacgcgta aaacggggat
     7441 cgacaacgat caaggttgca tcgttgttgt ttttcgcttc catcgcccag cggaaaccga
     7501 cgggatgcgc ttcagcagcg ttaccgccca ttaccattac gacgttagcg tttttgatat
     7561 ccacccagtg gttggtcata gcaccgcgac caaatgttgg agcaagactt gctaccgttg
     7621 gtccgtgtca gacgcgcgcc tggttgtcta ccgccagcat cccgagggag cgggcaaatt
     7681 tctgggtcag catcccggtt tcgttgctgg caccggaggc acacagcata ccggtagaaa
     7741 gccaacggtt taccgttacg ccctgctcgt tcttttcaat aaagttagcg tcacggtcag
     7801 ctttcatcag cttcgcaata cgggagaatg cttcttccca gctaatgcgc tgccatttgt
     7861 cagaacctgg cgcacgatat ttcgggtagc gcagacggtt ttcactgttg acgtaatcca
     7921 gcaaaccggc ccctttcggg cacagcgcac cacggcttac cggatgatcc gggtcacctt
     7981 caatgtgata aatcgcttct ctggcgtttt ttgcgccatc acccaggcta tacatcaata
     8041 gcccgcaacc tacggaacag tatgtgcagg tgttacggat ctctttagcg cgtaataatt
     8101 tgtagtttcg cgcctgagcc agtgcttgct tcggggcaaa gcccaatgcc gctaccgttg
     8161 ttccagccat accgcccgcg cagattttaa aaaattgtct gcgactgacg tccattgctt
     8221 tcctcttttt tcaggggtat tactgcgcga ggaaactaac agtaaaattc tgaatctttt
     8281 tgcgcgaaat caaaaagaac acgtgtttgc cacaaaataa tccgcctgcg caaattgacc
     8341 tacctcaata gcggtagaaa aacgcaccac tgcctgacag gccagttaaa aaaatgctat
     8401 aaaattcagc ttaattttta acggcaagag agacaaaaca gcgagcatga cacgacaaaa
     8461 agcaacgctc atagggctaa tagcgatcgt cctgtggagc acgatggtag gattgattcg
     8521 cggtgtcagc gaggggctcg gcccggtcgg cggcgcagct gctatctatt cattaagcgg
     8581 gctgctgtta atcttcacgg ttggatttcc gcgtattcgg cagattccga aaggctattt
     8641 actcgccggg agtctgttat tcgtcagcta tgaaatctat ctggcgcttt ccttagggta
     8701 tgcggcgacc cgtcatcagg cgattgaagt gggtatggtg aactatctgt ggcccagcct
     8761 gacaattctc tttgccattc tgtttaatgg tcagaaaacc aactggctga ttgtaccagg
     8821 attattatta gccctggtcg gcgtctgctg ggtgttaggc ggtgagaatg ggttacatta
     8881 tgatgaaatc atcaataata tcaccaccag cccattgagt tatttcctgg cgtttattgg
     8941 tgcgtttatc tgggcagcct attgcacagt aacgaataaa tacgcacgcg gatttaacgg
     9001 aattaccgtt tttgtcctgc taacgggagc atgtctgtgg atttactatt ttctcacgcc
     9061 acaaccagaa atggtattta gcacgcccgt catgattaaa ctcatctctg cggcatttac
     9121 cttaggattt gcttatactg catggaatgt cggtatattg catggcaatg tcaccataat
     9181 ggcggtaggc tcgtatttta cgcctgtact ttcctcagcg cttgcagccg tgctgctcag
     9241 cgccccgctg tcgttctcgt tctggcaagg cgcactaatg gtctgcggcg gttccctgct
     9301 ctgctggctg gcgacacgtc gtggttaaaa taattgccgg gtctacggcc cggcagttaa
     9361 tcagataaat tccgacaata ttaaaaaata aatttcaaat accagtaatt caccttatat
     9421 ctcatttcga cttcgatcac atatcgataa ataatattat tctatttttc tcattgtttt
     9481 taatcaccat tctattttaa aaacagtatg tcatttataa atgacgccgt ttaaaattcg
     9541 tttagaaaat acgtttaacg ataattaaaa ggattctgtt aaatgaaatt aaaaatagtt
     9601 gcggtggttg taactggttt gttagctgcg aacgtagcac acgctgccga agtctataac
     9661 aaggatggta ataaactcga cctttatggc aaggttaccg ctctacgtta ttttactgat
     9721 gataagcgtg acgatggtga taaaacttat gcccgtctcg gctttaaagg agaaacgcaa
     9781 atcaatgatc aaatgattgg ttttggtcac tgggaatatg attttaaagg ctataacgat
     9841 gaagccaacg gctcgcgcgg caacaagacc cgtctggcct atgctggttt aaaaattagt
     9901 gaatttggct ctctggacta tggtcgtaac tacggtgtcg gctacgacat tggttcatgg
     9961 accgatatgt tgccagaatt tggtggcgat acctggagtc agaaagatgt cttcatgaca
    10021 taccgtacca ccggtctagc aacctatcgc aactacgatt tctttggctt aattgaaggg
    10081 ctgaactttg ccgcgcaata tcaaggcaaa aataaacgta ctgacaacag tcatctttat
    10141 ggtgctgacg acacgcgtgc caacggtgac ggtttcggta tctcctcaac ttatgtttat
    10201 gatggctttg gtatcggtgc ggtatatacc aaatccgatc ggacaaatgc gcaggaaaga
    10261 gccgcagcta atcctctcaa tgcctccggt aagaatgcag aactatgggc cacaggtata
    10321 aagtatgatg ccaacaacat ctactttgca gctaattacg atgaaacatt aaacatgacc
    10381 acctatgggg atggttatat ctctaacaaa gcacaaagtt ttgaagtggt ggcgcaatat
    10441 caattcgact tcggcttgcg cccatcactc gcttacctga aatcgaaagg cagagatctg
    10501 ggccgctacg ccgaccagga catgattgag tatatcgacg ttggtgcgac gtatttcttc
    10561 aacaaaaata tgtcgaccta tgttgattat aaaatcaacc tgattgatga aagcgacttt
    10621 acccgtgccg tagatattcg caccgataac atcgtcgcta cgggcattac ctatcagttc
    10681 taacgtcctc tcctgaccag cccttctctg ggctggtttt tgatttaagt gctgctgtat
    10741 gactccttaa gtaaaaatga gcaattcttg atatttctgg tcattcacct caattaatat
    10801 tattccatat cctccgggga catcttacta taatatttat tgatgagtga ttatgaccaa
    10861 tattaataca gcttgtgtaa aaaataatgc cagttatcaa cttaataacg cattacctaa
    10921 caaagaaacc atctccagca atttttgtga acgactagaa caatggggta ataaatcggt
    10981 taataatggt gaagaaagag caattgccgt ggagcgaatt aaagaagctt acaattcgaa
    11041 catggcatct cttgatttgt catatcttga tttgagtgaa ttaccgccta tcccctcgac
    11101 agttaatacg ttaaatctgg aaaacaattg tctaacttgt cttgacttag ctgacaatgc
    11161 cagtctcgtt aatatcaacc tcagcgttaa taaaattaac acgataacct ttccaaaaga
    11221 accaaaactg gaaaacattt atattaatca taacaatctg gaaagtttag atttaaaaaa
    11281 tcagcattca ttggttaatc tggaagcgca aaataatagc ctaaaaaaat gatatttctg
    11341 atagttataa actgaaattt cttaatcttg attataacaa gctagcatcg ctggatgtct
    11401 cccggcaaga atctctgatt gagttaagtg cccatcacaa tatgatcact gaccttatat
    11461 tacacaatca tcccaggatg aaaacaatca ctttaaacga caaccatatt gcacatttaa
    11521 acgccaaaaa cactacaaaa ctggaatatt taaacttaag caataacaat ttactgccaa
    11581 ccaatgacat tgatcaacta atatcatcaa aacatctttg gcatgtatta gttaacggca
    11641 tcaacaatga tccacttgca caaatgcagt actggactgc agtaagaaat ataattgatg
    11701 acactaatga agtgaccatt gatttatcag gacttaattt aaccactcaa ccaccagggc
    11761 tgcaaaactt cacctctatc aatcttgata ataaccaact cacacatttt gatgcaacca
    11821 actacgatag actcgtaaag ctaagtctga atagtaatgc tcttgagtca ataaattttc
    11881 ctcaaggcag aaatgtaagt attacacata tatctatgaa taataatgct ctcagaaata
    11941 ttgatataga taggctttca tcagttactt attttagtgc ggcacataat caactagagt
    12001 ttgtgcaatt agaatcttgc gaatggctgc aatacctgaa tctcagccat aatcaattaa
    12061 ctgatattgt tgcaggtaat aaaaatgaac tcttactgct ggatctatcc cataataaac
    12121 taacaagttt acacaatgtc ttatttccca acttgaatac gttacttatt aacaacaatt
    12181 tgctttctga aattaaaata ttctatagca acttccgcaa tgttcagaca ttaaacgctg
    12241 ctaacaacca gttgaaatat ataaatcttg atttcctgac ttatcttcca tctatcaaaa
    12301 gtttaagact ggacaataat aaaataaccc acattgatac taataataca tccgatattg
    12361 gaactttatt ccccataata aaacagagca aaaacttaaa ttttttaaat gtttctggga
    12421 agaacaattg acctactatg cagctcatgt tatttaattt gttttcccca gcacttaagc
    12481 ttaatactgg cctggcaatt ctttcgcctg gtgcatttga agttcactct gacggaatag
    12541 atgcggataa cgaattgttt cactatacta ttaaaaaagc atatacccca tataatatac
    12601 acacttacaa gacagaggaa gttgtaaacc agaggaatat aaaagttaaa aacatgacct
//
LOCUS       DADLTY010000117        12651 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.117, whole genome shotgun sequence.
ACCESSION   DADLTY010000117 DADLTY010000000
VERSION     DADLTY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12651
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.117"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            27..1037
                     /locus_tag="JN334_002858"
     CDS             27..1037
                     /locus_tag="JN334_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAY7438940.1"
                     /translation="MVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWFDEEPP
                     YSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYTD
                     EQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIGGMDFG
                     WDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPHDGNQHE
                     KGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFNTCEPFF
                     EEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPAPIRPIAR
                     RT"
     gene            1038..3203
                     /locus_tag="JN334_002859"
     CDS             1038..3203
                     /locus_tag="JN334_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAY7438941.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3217..4128
                     /locus_tag="JN334_002860"
     CDS             3217..4128
                     /locus_tag="JN334_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HAY7438942.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4128..5423
                     /locus_tag="JN334_002861"
     CDS             4128..5423
                     /locus_tag="JN334_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HAY7438943.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5468..5704
                     /locus_tag="JN334_002862"
     CDS             5468..5704
                     /locus_tag="JN334_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438944.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5682..6182
                     /locus_tag="JN334_002863"
     CDS             5682..6182
                     /locus_tag="JN334_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HAY7438945.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6182..7600
                     /locus_tag="JN334_002864"
     CDS             6182..7600
                     /locus_tag="JN334_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438946.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7600..8448
                     /locus_tag="JN334_002865"
     CDS             7600..8448
                     /locus_tag="JN334_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438947.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8448..8903
                     /locus_tag="JN334_002866"
     CDS             8448..8903
                     /locus_tag="JN334_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HAY7438948.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8906..9598
                     /locus_tag="JN334_002867"
     CDS             8906..9598
                     /locus_tag="JN334_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HAY7438949.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9608..10939
                     /locus_tag="JN334_002868"
     CDS             9608..10939
                     /locus_tag="JN334_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7438950.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10940..>12651
                     /locus_tag="JN334_002869"
     CDS             10940..>12651
                     /locus_tag="JN334_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HAY7438951.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIE"
BASE COUNT         3405 a         3130 c         3335 g         2781 t
ORIGIN      
        1 tctttccgaa ccttgttgat caccttctgg ttaagcatca cacggctgat ggcgttgaag
       61 atggcatttc aatctgctac ttcaagccat actcgcaagg ccgtgctcgc tggcagggtg
      121 acacaatcca cggcgtgtgg tttgacgaag agccaccata cagcatttat ggcgaaggtc
      181 ttacccgtac caacaaatac gggcaattct caattctgac gtttaccccg ctgatgggga
      241 tgtctgacgt tgttaccaag ttcctgaaga accccagcaa gtcgcagaaa gtggtcaaca
      301 tgaccatcta tgacgctgag cactataccg acgagcagaa agagcaaatc atcgcatcat
      361 atcccgagca tgagagagag gcgcgtgctc gcggtattcc tacgatgggt agtggtcgaa
      421 tcttccagat accggaagag acgattaagt gtcagccgtt cgagtgtcct gatcacttct
      481 acgtaattgg cgggatggat ttcggatggg atcacccaca ggcgcaagtt cagctttggt
      541 gggataagga ggcagacaca atctacgttt cacgcgtgtg gaaggcgaaa gaaaaaacag
      601 ccgttcaggc gtggggagcc gttaaatcat gggcgcataa agtgccaacc gcatggcctc
      661 atgatggaaa ccagcacgag aagggcggcg gtgagcagct caaagggcag tatgcagacg
      721 ctggatttat gatgttgcag gagcatgcga catggcctga tggcggtaac gctgttgagc
      781 caggcatcac tgaattgcgc gacatgatgc tcgatggtcg cttcaaagta ttcaatacct
      841 gtgagccatt ctttgaggag ttccgcctct atcaccgtga tgaaaacggg aaaatcgtca
      901 agcttaacga cgacgtgcta tccgccgttc gctatgcata catgatgcgc cgcttcgcaa
      961 aaatgatgcg cgacatcaaa aaaccaaaag agaaaaagat accagcccca atcaggccca
     1021 tcgcacggag aacttaaatg gccgacgaaa acagactcaa ttccattctg tgtaagtttg
     1081 acgcagactg gatggcgagc gatgaagcca gaaccgaggc gacaaatgac ctgtatttta
     1141 gccgagtgtc gcaatgggat gactggctat caaactacac taccctgcaa tatcgcggac
     1201 aattcgatgt tgttcgcccg gaggtcagga aactggtcgc agagatgcgc cggaaccctg
     1261 tcgacgttct cttccgaccc aaagacggcg ctaatcctga tgcagccgat gtattgatgg
     1321 ggatgtatcg tactgatatg cgccataaca cggcaaagat tgccgttaac gttggcgttc
     1381 gtgagcagat agagtcaggc gttggtgcat ggcgtctggt cacgcagtac gaagacaacg
     1441 atccaacaag caacaatcag gtaatccgac gcctgccaat ccatgaagcc tgctcacacg
     1501 tcatatggga cgccaacagc aagcagatgg ataagagcga cgctaagcac tgcacggtga
     1561 ttaacgcctt gtcgcgcaat ggctggaaag agttcgcaga ggattacggt attgacccgg
     1621 ataccctgcc atctttccag aatcctaacg atacatggct attcccgtgg gtgtcgaatg
     1681 atgtcgtcta cgtcgctgag tattacgagg tagaagagga gaaggagaag gtcttcatct
     1741 accgcgaccc gctgacaggt gagccggtca gctattacca gcaggatatc aaagacgtca
     1801 tcgacgacct ggctaatcgt ggattcatta aggtagcaga gcgcaaggtg aagcgtcggc
     1861 gtgtgtataa gtcgatcatc acctgtacgc agatactgaa agaccgcgag aagatagccg
     1921 gagagcatat tccaatcgtt ccagtgtatg gcgaatggtc attcgctggt gacaaggagt
     1981 gctacgaggg tgtggtaagg ctgacgaaag acggtcaacg ccttcgtaac atgattatgt
     2041 cgttcaacgc cgatattgtt gctcggtcac cgaagaagaa accgaccttc ttccctgagc
     2101 aaatcgaagg ctacgaatac atgtacggcg gaaatgatga ctatccgtac tatctgcaga
     2161 acaggactga tgaaaacggt aacgacctgc cgattggtcc aatctcctac atggaaaacc
     2221 ctgaagtgcc gcaagccaac gcttacatgc ttgaggctgc caccaacgca gtgaaagaag
     2281 tggctagtct tggcgtggat gcgcaggcgg caaatggtca ggtcgctttt gataccgtca
     2341 atcaactgaa catgcgggca gaccttgaga catacgtgtt tcaggataac ctggctaccg
     2401 caatgcgacg tgatggcgag atttatgcct caatggtcaa cgatatttat gacgttcctc
     2461 gtcatgtaac gctgacactt gaagatggaa gcgagaaaga cgttcaactc tatgcgcaag
     2521 ttgtcgatta ccagtccggc aatgtggtca cactcaacga cattcgcggt cgatatgagt
     2581 gctatacaga cgttgggcca tccttccaga gtatgaagga acagaaccgc gcagagattc
     2641 aggagttgct caccaaggtt ccgcaaggta ctccagagtt ccagatgctg atgctgcaat
     2701 acttcacgct gcttgacggt aaaggcgtcg agatgatgcg agagtacgcg aacaagcaac
     2761 tggtgatgat ggggctgaag aaaccagaaa cacctgaaga gatggagatg gtgcagcagg
     2821 cacaacaaca gccgcagcag ccatcagcag agcaaattca ggcgcagggt atccttctgc
     2881 aaggtcaggc tgaattgctc aaggcagaga accaacaggc gcagattcag gttgaagctg
     2941 ccaaggttga agcccaaaac caactcaacg ccgcgaagat tgcagaaata ttcaacaata
     3001 tggacctcga caagcaggca gaactgcgtg agtacctcag gctcgtaggt caattccagc
     3061 aacagcgcag caaagatgct cgtgctaacg ctgagctgct tcttaaagat gcagaccaga
     3121 ctcattcaca acgcatggat ttcgcgaatc ttatgcgtca agttcaaatc ccctccggcg
     3181 gagtagccga gacacctcaa taagagagag ttaatcatgg accaaaccac cgacattcag
     3241 gcttctgaag aattaaccct gcccggcaat catgcagcgg catctgctga tggcttagtt
     3301 gtcgataatg ccaacgacag cgcaggtcag gaagaaggct ttgagattgt ccggaaagac
     3361 gatgagaaac caaaacaaga cccggcaact aatgctgaat ttgcccgtcg ccgcatcgaa
     3421 cgcaaacgcc agcgtgagct tgagcagcag atggaagcgg ttaagcgtgg agagttgccg
     3481 gagcacctgc gggtgaaccc tgagttacca aaacaaccag accctaacga ttatctttcc
     3541 gaagatgcac tggctaagta cgactatgac cagagccgcg cactggctgc cttccagcag
     3601 gcaaacagtg aatggcagat caaggctatg gacgcacgaa gccaggctgt cgccgagcag
     3661 ggtcgcaaaa ctcaggagtt cacccagcaa tcagcgcaat acgtcgaggc ggcccgtaag
     3721 cactacgagg cagcggaaaa gctcaatatc cctgactatc aggagaaaga ggatgcattc
     3781 atgcaactgg tgccgccagc agtcggtgcc gacatcatgc gcctcttccc ggagaaatcc
     3841 gccgctctca tgtatcacct tggtgctaat cctgagaaaa cacgccagtt gctggcgatg
     3901 gacgggcaat ccgcgctgat tgaactcact cgactgtcag aacgtttaac tctcaagcct
     3961 cgagccaagc ctgtttcaga agccccgtta cctgatgaac ccattcaggg acatgctgtt
     4021 gctgcaaata tctctgcgat tgaaaagcag atggaggcgg cagcaaacaa aggggatgta
     4081 gagacgtacc gcaagctcaa ggcgcaactg aataaaggaa ttcgataatg gcattaaatg
     4141 aaggtcaact ggtcacgtat gctctggatg aaatcatcgg aaccgtccag aacctgacgc
     4201 caatggcgtc aaaagtgaca aaataccccc ctccggcaga atccatgcag cgttcaagca
     4261 acaccgtgtg gatgcctgtt gagcaggaag cgccaaccca gactggctgg gatttaactg
     4321 gcaacgcaac cgggattctg gaactctccg tgaagtgcaa catgggcgat ccggataacg
     4381 atttcttcga gcttcgtgca gatgacctgc gtgatgagcg ttcttaccgt cgccgcatcc
     4441 aggcatccgc caaaaaactg gcgaataaca ttgagtcagc gattgccaaa caggcaactg
     4501 aaatgggctc gcttgttgtt cacgataccc gcgcaattgg tccatcgact ggcctgcctg
     4561 gctgggattt tgtgtctgat gcagagcgac tgatgttctc ccgtgagcta aaccgcgaca
     4621 tgggcatcag ttacttcctg aaccctgacg attaccgcaa agcaggccgc aacctggtag
     4681 atggtgacat cttcgggcgc gttcctgaag aagcatatcg aaacggtact attcagcgtc
     4741 agattgctgg ctttgatgaa attcttcgct caccgaaact tccggcagtt accaagtcaa
     4801 ccgcgactgg tgtaactgtt tctggtgcgc agaagtttaa gccgcaggca tacacccttg
     4861 ataccgatgg taacaaagag aacgtcgaca accgtgttgc aacggtgacc gtatcctcca
     4921 ccaccggatt taagcgcggc gacaaaatca gcttcattgg tgtgaagttc ctgtctcaga
     4981 tggcgaagaa tgtgctgact gatgacgcga ctttctcaat cacccgtgtg atcgatggta
     5041 ctcacatcga aatcacgccg aagccgattg cgctggatga cgcgtcactg acaaaagaag
     5101 agaaggctta cgctaacgta aacacctctc ttgctgatac cactccggta aacgttctga
     5161 acgtggcaac aaccaccgct aacgtgttct gggctgatga ctcaatccgc ctgctgtctc
     5221 agccgatccc ggtaacccat gaactgtttg ctggcatgaa aacgtcttcc ttcagcattc
     5281 caggtattgg tgttaacggc atcttcgcaa cgcagggtga tatcaacact ctgtctggta
     5341 agtgccgtat tgctgtgtgg tattcagcat gtgctgtacg accagaggca attggtgttg
     5401 gtctgcctaa ccagaccgcg tgataaccag agggagcttc ggctcccgtt tttatctgga
     5461 gacaagcatg acacacatga tctttcgtca tggcgacatg aagaagtgga aaggcgttgg
     5521 ctacgacttt gaaatcgtga aagccgaaga gcttcaggaa tatctggatg ctggctggtt
     5581 ttcacatcct gatgaccttt tgaaggatgt tgcagagcca gagccagagc cagaagaaaa
     5641 acagcgtaaa aagcctggtc gaaaacctaa ggcggcagca gatgaacctg acaacgaagg
     5701 gtgatttagt tcttgcggca ttacgtaagc tcggtgttgc atcaaatgcc acgttaaccg
     5761 atgtcgaacc gcagtctatg gaagacggcg tcaacgacct tgaaatgatg atggctgaat
     5821 ggcttggcgg tgatgcgtca cctggtatca acgttggcta catttttgct gatgcagatg
     5881 ttgctccgga tccgggcgat gagcacggtt tatcaaataa cgctatcaat gccgtcattt
     5941 tcaaccttgc ctgccgaatt gctccagatt atgcgctgga agcgtctgca aaacttataa
     6001 ccactgccag atacgggaaa gagcgactcg tcaaactgtc tgcaatggac agggcaaaag
     6061 cagctaaatg taagtccggt tatccaaacc gtatgcctgt tggtagtggt aaccagttgg
     6121 cgaagtggaa tggttggaat tacttccacc gaaaggaacc ttgcgataac gggagcgaat
     6181 aatgcctatt cagcaacttc cgcttatgaa aggtgtcggc aaagacttcc gaaacgccga
     6241 ctatatcgac tatctgccag tgaatatgct ggctacaccc aaagaaatcc tgaacagcag
     6301 cggatatctt cgctcattcc cgggcattgc caaacgttct gacgtgaacg gtgtatctcg
     6361 cggcgtcgag tacaacatgg cgcagaatgc tgtttatcgc gtgtgtggcg gcaagctgta
     6421 caaaggagaa agtgaagtcg gtgatgttgc cggaagtggt cgtgtatcaa tggcgcatgg
     6481 tcggacatca caggcggtag gcgttaatgg tcaactggtc gagtatcgct atgatggcac
     6541 ggtcaaaacc gtctcaaact ggcctacaga cagtggattc actcagtatg agttaggttc
     6601 agttcgcgac attacgcgct tacgtgggcg ttatgcgtgg tcaaaagacg gaactgattc
     6661 atggtttatc actgaccttg aagacgaatc tcatcctgac cgttacagcg cacaatatcg
     6721 tgccgagtct cagcctgacg gcatcatcgg tatcgggaca tggcgagact tcatcgtctg
     6781 ctttggttca tcgactattg aatatttctc cctgactggc gcaaccaccg ttggtgccgc
     6841 tttgtatgtt gcacaaccat cgctgatggt gcagaaaggc attgccggaa cttactgcaa
     6901 aacgccattc gctgattcgt atgcgtttat cagcaatccg gcaacgggtg cgccgtctgt
     6961 gtatatcatc gactccggtc aggtgtcacc aattgccagc gcgagcattg agaaaatcct
     7021 ccgctcctac actgctgatg aactggctga tggtgtgatg gaatcgctgc gatttgatgc
     7081 tcatgagttg ctgattatcc accttccgcg tcacgtcctc gtgtacgacg catcttcaat
     7141 cgccaatggc ccacaatggt gtgtgctgaa aacaggtctg tatgacgatg tgtaccgcgc
     7201 tatcgacttc gtttacgaag gcaatcagat aacgtgcggc gataagctgg aatctgttac
     7261 cgggaaactg caattcgata tcagcagcca gtacgacaag caacaggaac acctgctgtt
     7321 tactccactc ttcaaagcgg ataacgccag agtgttcgac cttgaggttg aatcgtcaac
     7381 tggcgttgcg cagtacgccg accgcctttt tctctctgca accactgact gcatcaatta
     7441 cgggcgtgag cagatgattg agcagaatga accgttcgtt tacgacaagc gtgttttgtg
     7501 gaagcgagta gggcgcatca ggaaaaatgt cggcttcaaa ttgcgcgtta tcacgaagtc
     7561 acctgtcact ctgtctggcg ctcagataag gattgagtaa tggctgattc gaatctcaat
     7621 gtgccggtaa tcatccaagc tacgcggctc gatacatcag tccttccacg caatatcttc
     7681 tcgcagtcgt atctgcttta cgttatcgca cagggcactg atgttggtaa cgtggctaac
     7741 aaagccaacg aggccggaca gggcgcttat gatgcacagg tcaggaacga tgagcaggat
     7801 gtgattctcg ctgaccatga gcagcgaatt tctgctgcgg aagcaacgct tgttaatcat
     7861 gaggagcgaa tcagacaggc agaatcaact cttcaggacc atgaaacacg aatagctcag
     7921 aatgaaagcg atatttcgtc gcttgacaca agagttcagt cgctggagtc gcaggtttca
     7981 gaccatgaaa cgcgcatcga tgctctggag tatgcaacca cacgcaagaa gtcagaggtt
     8041 gtttactctg gtgtatctgt aaccatcccg acagcgccga ccaaccttgt tagcctgctg
     8101 aaaacgctca caccgtcatc cggctcgttg gcaccattct tcgacaccgt taacaacaag
     8161 atggttgtgt tcaacgagaa caaaaccctg ctcttcaagt tgtcgattgt cggcacgtgg
     8221 cccagcggaa ccgccaacag gtcaatgcag ctaacctttt ccggctctgt tcctgacaca
     8281 ctggtcagca gtcgtaatgc ggcgacaaca accgacaaca tcctgttagc tacgttcttc
     8341 agcgtggata aagacggctt tcttgccaca aatggcagta cgttaaccat tcagtcgaat
     8401 ggtgcggcgt ttactgccac aaccatcaag ataatcgcgg agcagtgatg attacattca
     8461 aaccaacgcg aaacatcgac ctgatagaag cagtcggaaa tcaccctgac attatcgccg
     8521 ggagcaacaa cggtgatgga tacgactaca aacctgaatg ccgttacttt gaggtgaacg
     8581 tgcacggtca gtttggcggc attgtttact atcaggagat tcagccgctg acattagatt
     8641 gccacgccat gtacctgcca gagattcgcg gcttcagcaa ggaaattggg ctggcgttct
     8701 ggcgatacat tctgactaac accaccgttc agtgcgtaac atcgttcgct gcacgcaaat
     8761 tccgacacgg tcagatgtac tgcgcaatga ttggccttaa gcgtgtagga accatcaaga
     8821 aatacttcaa aggcgtggat gacgtgactt tttacagcgc cacacgcgaa gaactaatcg
     8881 acttcctgaa tcacgggaga tagccatgtt atatgcattt aagctgggca gaaaactgcg
     8941 cggcgaggaa ccttattgcc ctgaaaaagg cgggaaaggt ggcagttctg ataaaagcgc
     9001 aaagtatgca gcagaagccc agaagtatgc tgcagacctg caaaatcagc agtggcagac
     9061 gatcatgaaa aaccttgctc cgttcacgcc tcttgcggag cagtatgtta accagcttca
     9121 gaacctttcc agtttagaag gtcaggggca ggcacttaat cagtattaca actctaagca
     9181 gtataaagac cttgcagggc aggcgcgtta ccagagtctt gctgctgcgg aggcgacggg
     9241 tggacttggt tcgacagcca caagcaatca actggctacg atcgcgccga cactcggtca
     9301 gtcttggtta tcaaatcaga tgagcaatta caacaatctg gcaaacgttg ggcttggtgc
     9361 gctgcaaggt caggcaaacg ccgggcagac gtacgccaac aacatgagca gcattgcaca
     9421 gcaaagcgca gctcttgccg ctgctaatgc caataaacca tcaagtcttc agactgcaat
     9481 tagcggtggc acgtctggtg cgattgccgg tgcaggtctt gccagccttt tgggaacatc
     9541 aacgccttgg ggcgctggta ttggtgctgg tatcggattg cttggctcgt tgttttaagg
     9601 gggaatcatg gctacttggc aaggaacaaa cggcggattg ttggctggta tcggcggcgt
     9661 caactcaaac gctccgagcg taaatgacat cggcaatacg cttcagctta tcaggcagaa
     9721 caatgatatt gagcgttcag gcgctaacaa tgttgggctg actgctttgc aaggtctttc
     9781 aggtattgcg ggtgtttttc agcaggaaaa gcaggctcag cggcagaaag aatttcagca
     9841 ggcgtacgct aatgcttatg cgtctggtga tcgcggtgct ttgcgtcagt tggctactca
     9901 atatccagac cagattgaat ccgttcgtaa aggcatggga ttcattgatg aagagcagcg
     9961 taattctatc ggcaccttag cggctggcgc acgccttgcg tcatcgtctc cagaagcaat
    10021 gcaatcatgg ctgcaaaaca acgccggtga gttagctcgt gttggcgtta atcctcatga
    10081 cgtcgctcag atgtaccaac agaacccgcg gcagttcggc gaatttgtcg atcacctggg
    10141 gatgaacagt ctcgggcccg aaaaatactt tgacctacag gataaaatgc agggtcgcca
    10201 ggttaccatg cgcggtcagg atctggattc gcaaaccgcc gctcggaatc aggcaatcac
    10261 aatgcgcgga caagatatcc aggcgaattt aggtcagcag cgcattaatc tggacgcaga
    10321 aacaaaccgc attaacaacg aaaataagcg ccttgaccgg atgctatcag cagaaactaa
    10381 cgacctgaag cgccaggaaa tacagagccg catagcagcc aacaaccagc agttgcagca
    10441 gaagcagcaa gcgctaaatg atggctacaa agacggcatc aacaccctca caaccagcat
    10501 gttcactctg aacgatatcg ttagttctcc ttcacttaag agcattacag gcttacgtgg
    10561 agtaatcccc aacgttccag gctcacaggc tgcagacact caggcacgac ttgatacctt
    10621 taaatcacaa gcatacctga cagcggttca ggccatgcga ggcatgggcg cactttctga
    10681 tgccgagggc aaaaagctcg accaggctgt tggttcgctg cagaactcgc agagcgagga
    10741 gtcctttcgt cgcaacgctg gcgtcatcct gaacacgctc aaccagaagc gtaatgaggc
    10801 ggttggtaag tacgttcagc aaaacggtat caagcgagtg gaagcgcctc aggcttctat
    10861 agattacctg aagcagcacc ccgagctgtc aatcgacttc attaatcgct acggatatct
    10921 tccatctttg gggcagtaaa tggctaatta ccgtgatttg ttagagcagg ctggcgcacg
    10981 ttacggtgtg ccagaagggt tgatgactgc actgggtgcc aaggagtctt cttacaaccc
    11041 tgccgcagta agctccgccg gggctgtagg attgactcag gtcatgcctg ggacatggcg
    11101 tgatatgggt tataccgatg agcaaatgca aaaccccgaa tatcaggctg acgctggcgc
    11161 gcgctatctg gcaaagatgt accagcagtt tggtaactgg cgtgactctc ttcaggctta
    11221 tcacgacggt cccggcaacg ttatgaaggc aaagcgtggt gaatatacgc caggacctga
    11281 aggccgcggt tacgttgatg atcgctttgc tcaatgggct ggtgacccgg tgacagactc
    11341 aacagtcgaa cagcgcgcca cctctgcaaa ggtacatcct cagcaagacc ctaacaaccc
    11401 gtttgcacaa ctggaagtac agtcatccga acaagtatcg gcatcaggtg tgcagtcaga
    11461 cccaaataat ccatttgctc agattgagca gcaggcagcc agtcagcagc cacctcaacc
    11521 cgtaagttct gtcgcaccga aacctgttca gcaacaaaca gttaatcagg ccaataatgc
    11581 accaacacgg gaagaaccat cattgatgca acaagctggc gattggctca ctggtggtca
    11641 aagtgcaggg caaattgcag aacaggctgg tcgtggtctg gtaaacatac catttgacgt
    11701 attgcagggt ggcgcaagtc tgattaatgc aatcagtcag gggcttgggg ggccaaaagt
    11761 attggatgat gtttatcgtc cagtagacag accgacagac ccctacgcgc aagctggaga
    11821 gtcaataggc ggttatcttg ttcctggagc aggtgtagct ggaaacatgg tcattggttc
    11881 tctcgctgac gcggcgaatc aacggggtga ttttgccgaa aatgccgcta ttaatgccgg
    11941 acttagcatt gctacgcatg gcctgataaa tggcgttacc cgtggtgttc gtggtgcatc
    12001 aaatataatt agtggcaata aaacatctgc acagagagcg accactgcgc caacagaaac
    12061 atcaccattc tccggtgatg ccgctgcagc aacaaatcct gcggttcatg ccgcagaggc
    12121 aagagtagca caaggtgtac caatgacgcc tgcgacgaag aacccagagg aagtcgttcg
    12181 cacagtagca gcacaaaaaa ggccaaatct cgcttcatcg cttgatgaac tagatatcaa
    12241 tcctcaggct gaagttctgg agtctgctga aaggcttaat gttgattcat tactcccttc
    12301 acacttttcc gggaacgagc aatacaaggc agttgagcaa gcaatcaagt cccgtgcggg
    12361 ttctgctcta caggtgcagg aaaatgaagc aatcaggcag ctagcacagg gcgcgggtga
    12421 gataattgat cgcgtttccg gtgcaaaaga tgctcttggt atgagcgaca agtttattga
    12481 tacggtcaat ggaagaatgt ctgcgctgat gaaacgaagc gaccagcttt atcgcaatgt
    12541 tgaaaaggcg atgcctgcag gtgcaaaaat tgatgcgcca tcaacaaggt caatgctcaa
    12601 acaggtggca gaagatcttg gcgggatgaa aaaccttgac cctattgaaa a
//
LOCUS       DADLTY010000118        12634 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.118, whole genome shotgun sequence.
ACCESSION   DADLTY010000118 DADLTY010000000
VERSION     DADLTY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.118"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            758..841
                     /locus_tag="JN334_002870"
     CDS             758..841
                     /locus_tag="JN334_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HAY7438952.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="JN334_002871"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="JN334_002871"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAY7438953.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /locus_tag="JN334_002872"
     CDS             complement(2319..3161)
                     /locus_tag="JN334_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAY7438954.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="JN334_002873"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="JN334_002873"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAY7438955.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="JN334_002874"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="JN334_002874"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HAY7438956.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="JN334_002875"
                     /pseudo
     CDS             complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="JN334_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="JN334_002876"
     CDS             complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="JN334_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HAY7438957.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8830..9165
                     /gene="uspB"
                     /locus_tag="JN334_002877"
     CDS             8830..9165
                     /gene="uspB"
                     /locus_tag="JN334_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HAY7438958.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="JN334_002878"
     CDS             complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="JN334_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HAY7438959.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10967..12166
                     /locus_tag="JN334_002879"
     CDS             10967..12166
                     /locus_tag="JN334_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY7438960.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3109 a         3170 c         3356 g         2999 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca
     6421 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
     6481 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
     6541 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg
     6601 tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt
     6661 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
     6721 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
     6781 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
     6841 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
     6901 tatccagctt gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca
     6961 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
     7021 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
     7081 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
     7141 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
     7201 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
     7261 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
     7321 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
     7381 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
     7441 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
     7501 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
     7561 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
     7621 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
     7681 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
     7741 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
     7801 tattacaaca gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa
     7861 ttttgtattg gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta
     7921 cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag
     7981 gaacacgtcg tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc
     8041 aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg
     8101 gtggtgacca caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg
     8161 gcctaggtcg ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag
     8221 ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac
     8281 atcaataagc ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt
     8341 cgcattgtag gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga
     8401 gagatcgact gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg
     8461 tcgatgactg gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac
     8521 gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg
     8581 acccggatga tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga
     8641 atattatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc
     8701 tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac
     8761 tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg
     8821 ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt
     8881 aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat
     8941 ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac
     9001 aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat
     9061 gagtttattc gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt
     9121 ggtctggtgg tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa
     9181 taacgtataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca
     9241 ggaactgcaa ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa
     9301 acacccaggc catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca
     9361 ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat
     9421 aactcgccag gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg
     9481 tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca
     9541 tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga
     9601 tggtgctaag catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac
     9661 caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct
     9721 ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt
     9781 tcaggcggtt gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg
     9841 ttgcgccagc ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg
     9901 caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt
     9961 cgtagccagt ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca
    10021 acataaccag accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga
    10081 tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct
    10141 tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc
    10201 gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca
    10261 gagaaccgaa aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg
    10321 tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg
    10381 atgcaggtaa accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag
    10441 agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca
    10501 tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca
    10561 ccgccgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca
    10621 cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg
    10681 caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac
    10741 gttacgccat tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca
    10801 aagtgaaata tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt
    10861 atcttatata attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg
    10921 aggaatcttt acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga
    10981 tgccattatt ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggt
    11041 aggacgccgg gttctgctga tcgataatgg taaaatacta gggcgcaaaa tccttatgtc
    11101 tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca
    11161 gaatccgcat ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct
    11221 ggtcaataaa cacggtatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga
    11281 ctccgcgcag cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtaacctt
    11341 cagattgcgt agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct
    11401 gaacggcatg actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc
    11461 ggggctgggc gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct
    11521 gccgacccgc gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca
    11581 ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg
    11641 tgagaactta ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttcaag
    11701 ctactggcaa ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac
    11761 cttcctgaat gagcagcgta acgcacatcc gaatcaaagc ctgaaaaaca cactggcggt
    11821 tcatctaccg aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc
    11881 gctaaaacag ctcaacgtgt gtgaccaaca ggcactgatt agcacattga ccgactggcg
    11941 cgtacaaccc aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga
    12001 caccaacgaa ctttcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat
    12061 cggcgaagtg atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc
    12121 gagtgcatgg gcttgtgcgc aggatttggc gtcaattgtg aaataaccat gacgttttcc
    12181 cgtcagccgt ttgtatatgt cgttattaag tcattttggt acggttctgc atccgacatt
    12241 ctggtgttaa ctcgctgttt tgttagcgaa ttaacgctat tgccgttgtg gttattttat
    12301 agtgtattta ttctcagaga atatatatta ctgaaataaa aaacacaaag aataaccaaa
    12361 ggaaattatt tgaaacgtaa aaggtatata tttttaatct gtaaaattta aaatcacctc
    12421 ctctggattg aatcaacttg ctctatttaa gacgattgtt tttacatttt caccacctgt
    12481 atcaggtggt gtttttttat tggcggcaat gcctttatct tatttttatt acgttgtaaa
    12541 tatggctatt cctaaatgaa atttgatttg ctcagtttat ttgtgatgtc aggggagtgc
    12601 ttgccatgag agtcgtgata ttattattca tcat
//
LOCUS       DADLTY010000119        12588 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.119, whole genome shotgun sequence.
ACCESSION   DADLTY010000119 DADLTY010000000
VERSION     DADLTY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.119"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(28..285)
                     /locus_tag="JN334_002880"
     CDS             complement(28..285)
                     /locus_tag="JN334_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438961.1"
                     /translation="MKNYPTYGFYYPIANFIALQINGSKTGFAASITGFTQTISAGTI
                     SFLSVTVSDNGVGPGTTLGLGSLITSLLSIAVIAIAGNNKK"
     gene            741..1040
                     /locus_tag="JN334_002881"
     CDS             741..1040
                     /locus_tag="JN334_002881"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7438962.1"
                     /translation="MTKPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYES
                     QLYAWRSKQQQQMSSSERESELAAENVRLKRQLAEQAEELSILQKAATYFAKRLK"
     gene            1037..1892
                     /locus_tag="JN334_002882"
                     /pseudo
     CDS             1037..1892
                     /locus_tag="JN334_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1889..2350)
                     /locus_tag="JN334_002883"
     CDS             complement(1889..2350)
                     /locus_tag="JN334_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438963.1"
                     /translation="MDENALRFASYWRNSLADAESGKGCFERKDAQNFTHWHGIAAGR
                     LDEAIVNKFFKGEKDDVETVNVILRPKVYFRLLQHGKDRSAGTPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTDPTHVMWTRP"
     gene            complement(2865..3908)
                     /locus_tag="JN334_002884"
     CDS             complement(2865..3908)
                     /locus_tag="JN334_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAY7438964.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(4320..5531)
                     /locus_tag="JN334_002885"
     CDS             complement(4320..5531)
                     /locus_tag="JN334_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7438965.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            complement(5803..5897)
                     /locus_tag="JN334_002886"
     tRNA            complement(5803..5897)
                     /locus_tag="JN334_002886"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5861..5863),aa:Sec,seq:tca)
     gene            6190..7573
                     /locus_tag="JN334_002887"
                     /pseudo
     CDS             6190..7573
                     /locus_tag="JN334_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            7583..9901
                     /gene="yicI"
                     /locus_tag="JN334_002888"
                     /pseudo
     CDS             7583..9901
                     /gene="yicI"
                     /locus_tag="JN334_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
     gene            complement(9954..11663)
                     /locus_tag="JN334_002889"
     CDS             complement(9954..11663)
                     /locus_tag="JN334_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY7438966.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(11784..>12588)
                     /gene="xanP"
                     /locus_tag="JN334_002890"
     CDS             complement(11784..>12588)
                     /gene="xanP"
                     /locus_tag="JN334_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAY7438967.1"
                     /translation="KNLLLAGVVLALIILLNRQRNPYLRVASLVIAMAAGYALAWFMG
                     MLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLMLVFMITSLETIGDITATSDVSEQ
                     PVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCFGQNNGVIQLTGVASRYVGFVVA
                     LMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIAASGVRIVSREPLNRRAILIIAL
                     SLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGITAIVLNLIFPPEKQ"
BASE COUNT         3201 a         2966 c         3123 g         3298 t
ORIGIN      
        1 gtagatacat acgcagtcag agtacactta ctttttatta tttcctgcaa tggcgattac
       61 ggctattgac agtaatgaag ttattagtga accaagacct aaagtagttc cgggaccaac
      121 accgttgtcg cttacggtta ctgacaggaa actgattgta ccagcgctta ttgtttgagt
      181 aaaacctgta atagatgcgg caaatcctgt ttttgaacca tttatttgta atgcgataaa
      241 attggctatt gggtaataaa aaccataggt tggataattt ttcaatcttt gataattgat
      301 ttccgattat gaaaaataaa actgagaagg agaaaataat tgagtaacta aaagtactca
      361 ggccataagt atgcatgaaa attgatggtg atattgatat gtagcaaaat agagaactga
      421 gtattaatga gacaagtata gtgccgcaga taaactcttt ggtcacaaag gatgcactgt
      481 tttgacatat tactttctca tttgcatgag ttatctttgg tgataataac attattataa
      541 tactgaacag catcataaca agaaatataa cattccagtt ataatattca ataatcaatg
      601 cgcgaggtgc tactaatggt attactgcgc ttacagatga tatatcagaa agcgcccgtt
      661 ttagttttaa tggagggtga tcttacccac caatagtgga cacaggacta agtgagtaaa
      721 ctctcaacca gaggtgactc atgacaaaac cagtatcaat cagcaagaag ccccgtaaac
      781 aacatacgcc tgaatttcgt aacgaagccc tgaaactcgc tgaacgcatc ggtgtggccg
      841 ccgcagcccg tgaactcagc ctgtatgaat ctcagcttta tgcctggcgc agtaaacagc
      901 agcaacaaat gagttcgtca gagcgcgaaa gcgaactggc cgctgaaaat gtccgcctta
      961 aacgacaact ggcggagcag gctgaggaac tgtccatcct ccaaaaggcc gcgacatact
     1021 tcgcgaagcg cctgaaatga agtatgtctt tatcgaaaat catcgggcag agttcagcat
     1081 caaagcgatg tgtcgtgtac ttcgggttgc ccgcagcggc tggtatgtct ggctcaggcg
     1141 tcgtcaccag atgagcctgc gccaacagtt tcggctcacc tgcgatgccg ctgtccataa
     1201 ggcattcttt gaggcaaagc agcgatacgg tgctccccac cttgctgacg aactgccgga
     1261 gttcaatatt aaaaccattg ccgccagcct gcgtcgtcag gggctgcggg cgaaagccag
     1321 ccggaagttc agcccggtca gctaccgtgc acatggcctg cccgtattgg agaatctgct
     1381 ggagcaggac ttcagcgcca gtggcccgaa accagaagtg ggcgggtgac atcacgtact
     1441 tgcgtaccga tgagggctgg ttgtatctcg cagtagtcat cgacctgtgg tcacgcgccg
     1501 tcattggctg gtcgatgtca ccgcgaatga cagcacaact ggcctgtgat gcactgcaaa
     1561 tggcgttgtg gcggagaaga cgcccggaaa gcgtcattgt tcatacggac cgtggtggtc
     1621 aatactgttc aggggattat caggcaatgc tgaagcgaca caacctgcgt ggcagtatga
     1681 gtgcgaaagg ctactgttat gacaatgcct gtgtggaaag cttctttcat tcgctgaagg
     1741 tggaatgtat ccacggggaa cgctttagca gccgggaaat aatgcgggca acggtgttta
     1801 attatatcga gtgtgattac aatcgctggc gtcgtcacag tgcctgtggc ggtctcagcc
     1861 cggaacaatt tgaaaaccat aatctcgctt agggccgtgt ccacattacg tgggtaggat
     1921 cagtatgggt cgtcttgtat ttgtcatact gcccaatctc accaatcgaa aatgctcctt
     1981 ttggcaaagg ttcaagcagg tctctgggaa tggaggtcgc tggcgtcgga tataaaaaac
     2041 cctcacggct tagcaaggct ggcgtcacta tcggtgtaac aatatcaggc gtgcctgcgg
     2101 aacggtcctt accatgctgc agtaaccgga aataaacttt tgggcgtaag atgacattga
     2161 cagtttcgac atcgtctttt tctcccttaa aaaatttatt gacaatcgct tcgtcaagac
     2221 gtcccgccgc tatcccatgc cagtgagtga aattttgggc gtctttccgt tcaaaacagc
     2281 cctttcctga ctcagcatct gcaagcgagt tgcgccagta tgaggcaaac cttaaagcat
     2341 tttcatccat taccagtcct gtgactcaat gaaatccatt gatttatcga atataaaacc
     2401 accgtatgga caactattgt ccatattaaa aaagttggat gcaatgtttt gtttataatt
     2461 cgatatcaaa tgtactggtt gtacgaggtt cagaacacaa gcacattcaa cagtacgtca
     2521 ggactattga tttcataaca gcgaatacaa ttttttactg tttataatgg ctgcctgtgt
     2581 tggacgggga taccagttca accgaaaacg ccagaggagg ggattacccg ctggcagggt
     2641 aaatctgtgg tttttgatgc cagctataac gtgtttgacg ataaagtatt acgaaagaac
     2701 tttaatgagt tcatggagta attcgttaaa gtaatttaca acgctatcct tttgcaaagg
     2761 aaactttcag aaaataaccc cagggtaatc acctggggaa ggaacacagt gttattattg
     2821 gataataaat tattacttgt tttaaagtta cactaatgca atatctactg atactggatg
     2881 cttgaatgtt tcatccctgc tgcggaaaag ggcaatatcc agggccagat atattcaccg
     2941 ttcatatttc tgcatttctg tacaatttcc tcagaaagtg tcttgtcaat tcttttccac
     3001 aggtgctcct gctgtcttaa aatactttcg catatttcga aatcatcttc ctgaatcagc
     3061 atttcggcgc atttaatgat ttctgatcgt tcatgcagca cggaggaatg gttatagagc
     3121 cagtttacaa acagcaatgc ttccggatat tgtccccata atggtgagta tgttgcatag
     3181 taatcttctg ccgtatccag agagccggag aagtcatgcc acacagcaag tggaaatgga
     3241 tgcagatttc cgcacagcat ttctgcgatg taagtgtttg tggcaaagcc atggcgaaaa
     3301 gctgtggcgg ccttaacatg ttccccgttc agaaaatgca ggagaccgag ttcataccag
     3361 taaggtgaaa attcatcagc atgctctttc agtacactga aggctcgctc atgatcgccg
     3421 gtgcgtaaca gctctgaacc caacaaccag cgagccccct gattatcttc aggattgtat
     3481 gccagtattt tatcagtgag cataaccgca tcctgatggc gctgtaagtg cacatttgcg
     3541 agaattgctg cataaagcgc gcgcagataa ggccggtttt cgggatgcat ccagattatt
     3601 tcaccacaga aactttccgg gatgatacga ttacctgccg ccagtccttt aagagcggcg
     3661 ttcagtgcct tacgcggggc attctgttcc agaaatgcat atgccagatg agcgtgtata
     3721 tcaataaagt ccggctcccg ttgagcaagg cgtcggagtt tgttgatgta actcgtctcg
     3781 ctgatttcat cagcgtcgta tgcatcaagc acttcttcaa actcccccag caatgggcca
     3841 aaccaggtat ccggaaatac gaaataaccc ttgttatcgt gcacttcaaa gcataatcta
     3901 tcgttcattt tctgagcctg taaaacatgt ggtgatatag tctgagagca taaaagttga
     3961 gattctgtgt atcaagtcct gtcttggcac cgaatgaata ttgcttacga agccccttca
     4021 tgctttcctt gagaacaata attttatctt tatgtgaatc tcatttttta taatgatgtc
     4081 agatgattga aatgctaaat tatatctgaa tcaactattc ctttgctaaa ggtatcgaaa
     4141 taactgcaag gctgtatttt gtaattgcca atttaaaaat gtcaatttgt ttttaattat
     4201 gtttttgtaa taagtttgaa gacgcaaaaa aataatataa taacaaaaat cctccagcct
     4261 tgtaggccag aggataaaat acttaattga ggaaaacatc agggaaaagt atttctgact
     4321 taactttccc ggctggagag tgaatcaatg taatcagcgt accattgcat catttcacgg
     4381 cggccatcga gatacagggc atgattatag gtacccctga tggaattttt atccacatga
     4441 gctaactgca tttcaatcca ggcggaatta aagccttgtt catgcagaat tgtactcatg
     4501 gtgtgtctga aaccgtgccc tgtgagtctt cccttatatc caagtaattc aattacttgg
     4561 ttaatgcttt ccttactaat tggtttgcgg ggatcatttc taccgataaa gactagggga
     4621 tgatgttttg agattggttc aagctgctta aatagcataa tgacctgctc agagagcggc
     4681 acaatatgag gtcgcttcat cttcattact tctgccggga tctcccacaa ttttgtctca
     4741 aaatcgatat cttcccagcg tgcaaaacgc agttcttgtg ttcgtacgcc ggttaacata
     4801 atgatctgag tagctgtctt agtgattata cttccggtat aacccgccag atcgttgagg
     4861 aagtggggaa gttcattagc agtaagaaag ggaaaatgta cttttttcgg tgtagctaaa
     4921 gcactggcaa gatcgggggc tgggttatag tctgcccggc cagtaacaat tgcatagcgg
     4981 aacacctcac cacagcgctg gcggaccttg cgcattttct ccagagctcc acgtttttcc
     5041 attttacgca gtgcttccag taacttcatt ggtttgattt cggcaatagg gcgcttgcca
     5101 atgtagggaa aaatgtcttt ttcaaaggta tcaatgattt catcacgata gcgtaatgac
     5161 caccgatcag cttttaactg gtgccattcg cgagcaatag cctcgaacgt atttttcaga
     5221 ctcatttgct gcgcgatttt ttcttccttt tttgcctccc ctggatcgcc tccagcagcg
     5281 aggagtttcc tggcttcact gcgtttctcg cgagcatcgg ctagcgttac atcggggtat
     5341 acgccaatag aaagcatctt ttctttacca gcaaagcggt acttcatgcg ccagtattta
     5401 gaaccattag tgttaatcag tagatacata ccgccgccat cagcaagctt atagggtttg
     5461 tctttgggtt ttgcggtttt cactttgata tcgttaatgc catgacatcc tccggctttt
     5521 gatgaattca tgttcctgag ggggatttct gccagtaact aacctgatac ccccaataca
     5581 ccccttgtat aacaaagata ttgttaaaag ccgttgttat gtatcacatt tttcctatta
     5641 tgggggtgct tgtatttcaa taggaaataa caccccctaa agtaccccca aaagaaactt
     5701 gatacggggt gaagtaggtt gacctcagga tacaaataaa tactgatata caagggatta
     5761 gaagagattt attgacattg ggagagttca gaagaggtaa actggcggaa gatcacagga
     5821 gtcgaacctg cccgggaccg ctggcggccc caactggatt tgaagtccag ccgcctcacc
     5881 ggagacgacg atcttccgcg cctcgattgc tacatggagg cggggcgcat tatagctact
     5941 tccttgagtt tctacatccc ccagatcagt tttttccgcc taataaacgc agcgtattat
     6001 cacgaaagca ttataattta tataaaaccc atattttaca atgatatatg agcggaatta
     6061 acgctaccac gatcacataa aacaagaatc ataaattaat aaccagatat cggaatattc
     6121 gctctcacag ggatggttta caaaatgcgt tatcggtctc agcaccccta tagcatcaag
     6181 gaaaagcaga tgaggagtga agtgttgtcc gttaaagaga aaattggtta tggcatggga
     6241 gacgccgcca gccacattat tttcgataac gtaatgttat atatgatgtt cttttatacc
     6301 gatatttttg gcattcctgc cggatttgtc ggaaccatgt ttttggtcgc tcgtgcactg
     6361 gatgcgattt ccgatccttg catggggttg ttggccgatc gaacgcgctc tcgctggggt
     6421 aaatttcgtc cgtgggtact gtttggcgca ctgccattcg ggatcgtctg tgtactggcc
     6481 tatagcacgc cagatctcag tatgaacggc aaaatgatct atgcagcaat tacttacacc
     6541 ctaacttacc ttactttata ccgtcgtcaa tatcccttac tgcgcattgg gtggtgtaat
     6601 caccaatgac ccgactcagc gtatctcgct gcaatcctgg cgttttgtgc tggcgaccgc
     6661 gggaggcatg ctttctactg ttctgatgat gccactggtt aatttaattg gcggtgataa
     6721 taaaccactc ggtttccagg gcggtatcgt ggtcctttcc gtggtggcat tcatgatgct
     6781 ggcattttgt ttcttcacca ctaaagaacg cgttgaagca ccacctacaa caacgtctat
     6841 gcgggaagat ttacgtgata tctggcaaaa cgaccagtgg cggattgtcg gtttactaac
     6901 cattttcaat atcctggcgg tgtgcgtacg cggtggggcg atgatgtatt acgtcacatg
     6961 gattttgggc acgccggaag tgtttgtcgc ttttctcacc acttattgcg tgggtaacct
     7021 gattggttcc gcactggcaa aacctctgac cgactggaaa tgtaaagtca ctatcttctg
     7081 gtggacgaac gccctgctgg cagtgattag cctcgcgatg ttctttgttc ccatgcaggc
     7141 cagcatcact atgtttgtct tcatcttcgt gattggtgtg ttgcatcaac tggtgacacc
     7201 tatccagtgg gtaatgatgt tcgataccgt cgactacggc gagtggtgca atggtaaacg
     7261 cctgaccggg atcagttttg ctggcacgct gtttgtgctc aaactggggt tggccttcgg
     7321 cggcgctctt atcggctgga tgctggctta tggcggatat gatgcggcag aaaaagcgca
     7381 gaacagcgcc acgattagca tcattattgc gctattcacg attgttccgg cgatctgtta
     7441 tttgctgagc gcgattatcg ctaaacgcta ctactcactc acgacgcaca atctgaaaac
     7501 cgttatggaa cagctggctc agggtaaacg ccgttgccag caacaattca cctctcaaga
     7561 agtgcagaac taaggaacgg caatgaaaat tagcgatgga aactggttga ttcaacctgg
     7621 cctcaatttg attcacccgc tttaggtatt cgaggttgaa cagcaggata atgaaatggt
     7681 ggtctatgct gccccccgtg atgtgcgtga acgtacctgg cagcttgata cgcctttatt
     7741 tacgctgcgc tttttctccc cacaggaagg tattgtcggt gtgcggattg agcattttca
     7801 gggggcgctg aataacggtc ctcattatcc gctcaatatt ttgcaggacg tgaaggtcac
     7861 aatcgaaaac acagaacgtt atgctgagtt taaaagtggc aacttaagcg cgcgtgtcag
     7921 caaaggtgag ttctggtcac tggattttct gcgcaacggc gaacgtatta ccggtagtca
     7981 ggtgaaaaat aatggctacg tgcaggacac gaataatcaa cgcaattata tgtttgagcg
     8041 gcttgatctt ggcgttggcg aaacagttta cggtctggga gagcgcttta ctgccctggt
     8101 gcgcaatggc cagacggtag agacctggaa ccgggacggc ggcacaagta ctgaacaggc
     8161 gtataaaaat atcccgttct acatgactaa ccgtggttat ggggtactgg tcaatcatcc
     8221 ccagtgtgtc tcttttgaag tgggatcgga gaaagtctcc aaagtgcagt tcagcgttga
     8281 gagtgaatat ctcgaatact ttgttatcga cggcccgacg ccgaaagcgg tacttgatcg
     8341 ttatacccgc tttactggtc gtccggcgct gccgcccccg tggtccttcg gcctgtggct
     8401 aaccacttca tttaccacca actacgacga agcgacggta aacagcttta tcgatggtat
     8461 ggcggaacgc aatctgccgc tgcatgtttt ccactttgac tgtttctgga tgaaagcctt
     8521 ccagtggtgc gattttgagt gggacccgct gactttccct gacccggaag ggatgatccg
     8581 ccgcctgaaa gcgaaagggc tgaaaatctg cgtctggatt aacccctata tcggtcaaaa
     8641 atcccccgtc tttaaagagt tacaagagaa aggctattta ctcaaacgcc cggacggttc
     8701 gctatggcag tgggataaat ggcagccagg tctggcgatt tatgacttta ccaatccgga
     8761 tgcctgcaaa tggtacgccg acaaactgaa aggtctggtc gcgatgggcg ttgattgctt
     8821 taagaccgac tttggcgaac gtatcccaat tgatgttcag tggtttgacg gttccgattc
     8881 gcagaaaatg cataaccatt atgcgtacat ctacaacgaa ctggtgtgga acgtgctcaa
     8941 ggacaccgtt ggtgaggaag aagctgtctt gtttgcccgc tcggcctccg tcggtgcgca
     9001 gaaattcccg gtacactggg gtggcgattg ttacgctaac tacgaatcaa tggcggaaag
     9061 cctgcgcggt ggtttgtcta ttggcctttc aggttttggc ttctggagcc acgatatcgg
     9121 cggctttgaa aataccgctc cggcgcacgt ttacaaacgc tggtgcgcgt ttggtttgct
     9181 ctccagccat agccgtttac acggtagcaa atcttatcgt gtgccgtggg cctacgatga
     9241 tgagtcctgt gatgtggtgc gcttcttcac gcaactgaaa tgccgcatga tgccgtatct
     9301 gtatcgtgaa gctgcgcgtg cgaacgcgcg gggtacgccg atgatgcggg ccatgatgat
     9361 ggagttcccg gacgatccgg cttgtgatta ccttgaccgt caatacatgt taggcgacaa
     9421 cgtgatggtt gcgccggtgt tcactgaagc gggcgatgtg cagttctacc tgccggaagg
     9481 tcgctggaca cacctgtggc acaacgatga actcgacggt agtcgctggc ataaacagca
     9541 gcacggcttc ctgagtctgc ccgtttatgt gcgtgataac actctactgg cgctgggcaa
     9601 caacgatcaa cgtcccgatt acgtgtggca cgaaggcacg gcattccacc tctttaatct
     9661 gcaagacggg catgaagccg tctgtgaagt gcctgctgct gacggatcgg tgatctttac
     9721 tttaaaagca gcacgtactg gcaacacgat tactgtgact ggtgcgggcg aggcgaagaa
     9781 ctggacgctg tgcttgcgca atgttgtgaa agtaaatggt ctgcaaggcg gttcgcaggc
     9841 tgaaagtgag caggggctgg tggtgacgcc tcaagggaat gcgctgacaa ttacgttgta
     9901 atctattgac ggaatgccgg gtacgtctga gtcgtacccg gcgagattga tgtttaccgc
     9961 accggctccg tcgatgtaac ttcaccgctg gaaacgacgc ccgcattcat ggtttgagtc
    10021 acttgctgct ttgcggcgtt cacggcatgc aattgcccgc taaccgtagg tttcagcggg
    10081 gcattggcct gaatatctcc actggcggta agctgaatat tgccgtcgcc agtcaacggt
    10141 aattcaggcc atccccattg ttgcaaaata ttcaccggca caccgcgtcc attcaggctg
    10201 atatgggtct gacgttgtgg cgtttgtgaa acacttgcgg tggcttccag aatgcctttt
    10261 tcagtaaatg cactcagttc gctgatattc accgtgctgc tgttggcggt cagcgccagc
    10321 gacgggcgac gaacatcaac acgattgaat gtcgcggcgg cggcattcag attcgccgag
    10381 ccactccaga cgccccattt atgatcggta accagcgtca ggttggcacc gtaaccatcg
    10441 agcgcggtga gctgccacgg gaagtcaggg tcgatatcaa tgatcagatt gcggctggcg
    10501 ctaaatctct tcagttgcag gctgtttaac caaccgggtg tcgtttccat ccacaactgt
    10561 tgccagtttt tcggcaaggt atattccagc ccggcaattg ccgcatcatc aaggatcaac
    10621 gttttaccgt cacgcagcca gttccctgac gttctgacca tacccccttc ccagcggctg
    10681 gtgaactggc gcagcgctac gccctgcggg gaaaattccg cgtttataat cgggtcaaat
    10741 aaatgcagcg aaccataaat gaactcgctg gcgttcatcg acagtttgcc ttcctgtgtc
    10801 tgccagtcat ctttactgaa ggtcatgttg cgcaagctga gatcgaggtc ggtcaccgcc
    10861 cagtccggac cttgcagacg agcatcgatg acttccaagc gaccaatctg caacgacggg
    10921 acagagcgta atggcgcaaa gaagtcggtt agcgattttt cgctttgtag acggatatcc
    10981 gccatgcgca gattttccac ttgccagctg ccgtcggcgt tacgctgcgc gtttccggtt
    11041 aatgtcccgc gggcgatgtc ggcacccagg ttagtcagcg taacgcgatc gttatcaata
    11101 ctgccttcaa tcagtacatt ggtggcagga acatcattga gcgaaagcga tccggcacta
    11161 aactgaatct gcgccttcgt acccagcact ttaccggctt ctggtgacca cggaaccacg
    11221 ccgccattta cccgctgcgc gctcagtttc cattcgctat tcgggctatt aaacgccata
    11281 tcacgcagtt gcagacgatc ggctttgaac ggtagcggcg cggtctggtc ggtgagattc
    11341 agcgtcccgt tttccagcag gatggtatcg acatggcgtg gttcggtcag ttggcgactg
    11401 cttagcgcaa tgtcgacact ttttgccacc aggggcgcgg gctgaccatc acgaccaaac
    11461 gtgacattct ccagcacgat atgagatggc gcggaaaaac ggtgatccat cgccccgaag
    11521 gccagatgat agtcgctatt ctcggaaacc catgcgctga tatgttctgc tccccagcgg
    11581 gtttgcagaa gaaaatagag gccagcgatc accactaaca gagcgatgag aatgtagaga
    11641 agcagcttcc caataaattt catggtcttc catcccgtga aatgcacata agggagttat
    11701 gcacgattta cgcgcaatcc tcaaggcggg aatggtgaaa gaggtgagac tgcggcggta
    11761 attatcttac cgccgccagt gaattactgt ttttctggtg ggaaaatcag attcagcacg
    11821 atggcagtaa taccgcccgc ggcgatcccg gaggagagca ggtttttcag ccattcaggg
    11881 gcaaactgca aaatcagcgg ctgctgagac acgcccagac caaccgccag cgacagcgcg
    11941 ataatcagaa tcgcccgacg gttcagcggc tcacgagaaa cgatacgcac accggaggcg
    12001 gcgatggtgc caaacattac aagcgttgcg ccgcccagaa ccggttctgg aatgtgttgt
    12061 acaaaaccgc tcactgccgg gaacagaccc agcacgatca acatcagcgc gacgacaaaa
    12121 ccgacatagc ggctggcaac accagtcaac tggatcactc cgttgttttg cccgaagcag
    12181 gagttcggga aggtgttaaa caccgccgaa acaaacgagt tcaggccgtt tgccagcacg
    12241 ccgcctttca ggcgtttcat gtacagcgga ccggacactg gctgttcgga aacgtcagag
    12301 gtcgccgtga tatcgccaat ggtttccagc gaagtgatca taaagaccag catcagcggc
    12361 agcagcagac tccattcaat gccaagaccg taatagagcg gcgttggcac cataatcagt
    12421 tcttgcgtca tcggttcgtt gctttctggc aacatgccca taaaccacgc cagcgcatat
    12481 ccggccgcca tcgcaattac cagtgaggcc acgcgtaagt aagggttacg ttgacggtta
    12541 agcaggataa ttaaggctaa aaccacgcct gccagcagca gatttttc
//
LOCUS       DADLTY010000120        12550 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.120, whole genome shotgun sequence.
ACCESSION   DADLTY010000120 DADLTY010000000
VERSION     DADLTY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.120"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(2..94)
                     /locus_tag="JN334_002891"
     tRNA            complement(2..94)
                     /locus_tag="JN334_002891"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="JN334_002892"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="JN334_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAY7438968.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JN334_002893"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JN334_002893"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAY7438969.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JN334_002894"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JN334_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAY7438970.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JN334_002895"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JN334_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAY7438971.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JN334_002896"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JN334_002896"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HAY7438972.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="JN334_002897"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="JN334_002897"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAY7438973.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="JN334_002898"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="JN334_002898"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="JN334_002899"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="JN334_002899"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAY7438974.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="JN334_002900"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="JN334_002900"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAY7438975.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="JN334_002901"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="JN334_002901"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAY7438976.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="JN334_002902"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="JN334_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HAY7438977.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="JN334_002903"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="JN334_002903"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HAY7438978.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="JN334_002904"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="JN334_002904"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HAY7438979.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
BASE COUNT         2913 a         3407 c         3158 g         3072 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt
//
LOCUS       DADLTY010000121        12531 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.121, whole genome shotgun sequence.
ACCESSION   DADLTY010000121 DADLTY010000000
VERSION     DADLTY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.121"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..955)
                     /locus_tag="JN334_002905"
     CDS             complement(<1..955)
                     /locus_tag="JN334_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY7438980.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQDDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAG"
     gene            1201..1827
                     /locus_tag="JN334_002906"
     CDS             1201..1827
                     /locus_tag="JN334_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY7438981.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1824..1979)
                     /locus_tag="JN334_002907"
     CDS             complement(1824..1979)
                     /locus_tag="JN334_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY7438982.1"
                     /translation="MSVNGDKVTEDGRILTDRGQRIRDVRTGPDGYLYVLTDESSGEL
                     LKVSPRN"
     gene            complement(2000..2938)
                     /locus_tag="JN334_002908"
     CDS             complement(2000..2938)
                     /locus_tag="JN334_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005067270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY7438983.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGS"
     gene            complement(3049..3432)
                     /gene="bssR"
                     /locus_tag="JN334_002909"
     CDS             complement(3049..3432)
                     /gene="bssR"
                     /locus_tag="JN334_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HAY7438984.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEVWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3645..4970
                     /gene="rimO"
                     /locus_tag="JN334_002910"
     CDS             3645..4970
                     /gene="rimO"
                     /locus_tag="JN334_002910"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAY7438985.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5017..6345)
                     /locus_tag="JN334_002911"
     CDS             complement(5017..6345)
                     /locus_tag="JN334_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7438986.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFGGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6353..8695)
                     /locus_tag="JN334_002912"
                     /pseudo
     CDS             complement(6353..8695)
                     /locus_tag="JN334_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8873..9784)
                     /gene="gsiD"
                     /locus_tag="JN334_002913"
     CDS             complement(8873..9784)
                     /gene="gsiD"
                     /locus_tag="JN334_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HAY7438987.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LDIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9787..10707)
                     /gene="gsiC"
                     /locus_tag="JN334_002914"
     CDS             complement(9787..10707)
                     /gene="gsiC"
                     /locus_tag="JN334_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HAY7438988.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10725..12263)
                     /gene="gsiB"
                     /locus_tag="JN334_002915"
     CDS             complement(10725..12263)
                     /gene="gsiB"
                     /locus_tag="JN334_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HAY7438989.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12283..>12531)
                     /locus_tag="JN334_002916"
     CDS             complement(12283..>12531)
                     /locus_tag="JN334_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438990.1"
                     /translation="IVEIGSRCAVFENPQHPYTRKLLAAVPVAEPSRQRPQRVLLSDD
                     LPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
BASE COUNT         3322 a         3261 c         2825 g         3123 t
ORIGIN      
        1 agcccgcttc gcctgccccg agattcactt cgatcttatc accaaaccag acgcgctgag
       61 tcacaaaggt ggcatcaggt ttaattggcg tcacagtttc aaagaagcgg aaaccccagg
      121 tcaataattt ctcagactca ttaaaacgga tacggtcagt tttcgccccc agcactacgg
      181 agattaaacg catatcatcc tgggtagccg aagcaaccag attatatccc gcacctgccg
      241 tggttcctgt cttcatgcca tcaacattca gattgctgct ccacagcaga cggttacggt
      301 taggctgacg aattttgttg aaggtgaact ctttctcttt atgaatggcg tactcttccg
      361 gcacatcgtg gatcaatgct ttacccagca atgccatatc gcgcgcggta ctgaactgcc
      421 ccggcgcatc caggccgtgc accgtctgga aggtagtgtt ggtcagcccc agttttttgg
      481 cataaccatt catcagacca ataaaggact cctggctccc ggcaacgtaa tctgccagcg
      541 caatacaggc gtcattaccg gactggataa tcacaccttt gttcaagtct gccaccgaaa
      601 cctgatcgcc cggtttgagg aacattaccg atgaaccacg cagtgccgga tttcccgtcg
      661 cccaggcatc tttaccgacc gtcaccatat cggtgagttt aatcttatcg gccttaagcg
      721 cctgcccaac cacatagctg gtcatgattt tagtcaggct cgcaggatcc agtttctcat
      781 ccgcattgcc ttctgccagc actttaccgc tggcgtaatc cattaaaatc catgcacgcg
      841 catccacgct cggcgcttca acggtttgtt cggccgcgaa tgccgttggg gcaaaaagga
      901 ataaaaatgc agaacccgct gcaagaccac gaaggagaga ggagtattgc gtcataaatg
      961 ccacccgagt atccattcaa aaaaatacgt tatatcgccg tatgataagt tcggtgatta
     1021 ataacgcaca cacaaactta aagaaaccgt aagttggtaa agtttttaaa gtttatgcaa
     1081 tatcctgccg cttcgctgct tctggtgtca tttttttgcc agctgttgct taaaagttaa
     1141 ctttacttca ccatcggagt cctgttcagt ctgtggctgc ctgtcggaaa aggggtgagt
     1201 atgattacgc tgtggggtcg gaataattca actaacgtaa aaaaagtatt gctgacgctc
     1261 gaagaactgg aactacctta tgagcaaatt ctcgcgggcc gtgagtttgg gataaatcac
     1321 gatgctgatt ttctggcgat gaaccctaac gggctggtgc cgttgttgcg tgacgacgaa
     1381 agtgatctca ttctttggga atcaaacgcc attgtccgct atctggcggc acagtacggg
     1441 caaaaacgcc tgtggatcga ctcaccggca cgtcgtgcgg aagcggaaaa atggatggac
     1501 tgggcaaacc agacgctcag taatgctcat cgcgggatcc tgatgggatt agtcagaaca
     1561 ccaccggaag agcgcgatca ggccgccatt gatgccagtt gcaaagagtg cgacgccctg
     1621 tttgccctgc tcgatgcgga actggcaaaa gtaaaatggt tctctggcga cgagtttggt
     1681 gtgggcgata tcgctatcgc accgttcatc tacaatttgt ttaacgttgg cctgacctgg
     1741 acaccgcgtc caaatctgca acgctggtat cagcaactca ctgaacgccc cgcagtgcgc
     1801 aaagtggtga tgattcccgt tagctaattg cgtgggctaa ctttaagtaa ttccccactg
     1861 gactcgtcgg tgagaacgta taaataaccg tcgggtccag tgcgaacatc acgaattcgc
     1921 tgccctctgt ccgttaaaat acggccatct tctgtcactt tgtcgccgtt gacgctcatc
     1981 acaatgacat ctttatcttt cagctcccaa taaataattt ttgctgccac tgggggaatt
     2041 tatcgctgtt atagaaggcc atgccgctca cagcgggcga atctttccag taaaaaacag
     2101 gttgctcggt cccggcgacg atctcccctt tcgcttccgg tatcttaaag cctgaatagt
     2161 tgattcccca ggttgccagc ggccagccgt agtttttgcc tttttgcggg atattaattt
     2221 catcaccacc gcgcgggcca tgttcattca gccacagtgc attactccac ggattcatcg
     2281 ccattccttg cggattacga atgccataag accagatctc ggcgcgcgca ccggattcct
     2341 ttataaaagg attatcatcc gggatttcgc cctggtcggt cagacgcacc agtttgccct
     2401 gtaatttatc cagatcctgc gccgtcgggc gctgattgtt ttcgcccaga gcaataaaaa
     2461 gataaccttt accgtcgaat accagccgcc cgccaaaatg gttgccggta gacagttttg
     2521 gcatctggcg gaagacggtg cggaagtcgg tcacttttga gagatcatca cttaagcggc
     2581 cataacccac agcagttccg gctttgccat catcgccaac ttcggaataa cttaaccaga
     2641 tgcggcgaga ctgagcaaaa tcaggcgcta aaaccacgtc cagcaggccg ccctgcccgt
     2701 gcgcccaaac gtccggaact ccggaaagcg gcgcagataa tccttttcct gcttgccagt
     2761 gacgcaactc gccgccgcgc agagtgatta acataccgtg attatcgggt aaaaaggcca
     2821 gtgcccaggg atggtcgagt ttgtcttgca gtacttcgac atttaccgtt gcaggagccg
     2881 cccagagagc ggaagaaaga caaataaggg gcacaaggaa aaaggattgt cgatgcataa
     2941 cgcctccgga ttgactctgg cttaaagcgt agtcagtgga ggagataaca aattcatttt
     3001 tacaaaaact taaacatgaa gggggagacg ctttctcccc cttagttttc aggccttctc
     3061 aagcatggcg tgcttctgca ggctctggat actcagcgtt aagctcatca gacaattttc
     3121 aagcttatcg gcgttgacgg taataacagt cgggcaatca tggtgcccac tcatcaaaca
     3181 tactgcggct gtcgctaatg cttcttcagc atgatgaaga gcactccaca cttcctgatc
     3241 cagatgaaga ttcaaccgca gcgatttatc gtgcagttcg cgattcagtt taaaaaagtt
     3301 atctcgtaga tgattgcttt cgctgacgga catgtatcct tttgccttcc tcagttgcac
     3361 gtatgcagcg aggtcaacgc gtgcgtcgat caaccggttc agcagatcga ttcgctgtct
     3421 gtcaacgaac atacttcgat cctcctcttt ccagccttgt tgcaattaaa tagtatggct
     3481 attttttgag caaaatgctg tccaggatca attcttcacg ccgaattaac aaaatttaca
     3541 tttccttcgc ctccgccagc gaatgagatt gcgatacaaa ttttatccag aactctgtag
     3601 aatccctgcc ctgacctttc ctgtaactga atatttttta agctatgagc aaagtaactc
     3661 cccagccgaa aatcggcttt gtttcccttg gctgtccgaa aaaccttgtc gattcagagc
     3721 gtattctcac cgaactccgc actgaaggtt atgacgtggt accgagctat gacgatgcga
     3781 acatggtgat cgtcaacacc tgcggcttta ttgacagcgc ggtacaagaa tcactggaag
     3841 ccattggtga agcgttgaat gaaaacggca aggtaattgt gaccggttgt ctgggggcaa
     3901 aagaagatca gatccgcgaa gtccacccga aagtgctgga aatcaccggg cctcatagct
     3961 atgagcaggt tctggagcac gttcatcact acgtgccaaa accgaaacac aacccattcc
     4021 tgagcctggt gccagaacaa ggtgtgaaac tgacgccgcg tcattatgcc tatctgaaaa
     4081 tttcggaagg ctgtaatcac cgctgcacct tctgcattat tccgtctatg cgcggcgacc
     4141 tgatgagccg tccgattggc gaagtgttaa gtgaagcgaa acgtctggta gatgcgggcg
     4201 ttaaagagat tctggtgatc tcgcaggata cttccgccta tggcgttgat gttaaacatc
     4261 gtactggctt ccacaacggc gagccggtaa aaaccagcat ggtcagcctg tgcgaacagt
     4321 tatcgaaact ggggatctgg acacgtctgc actacgttta cccttatccg catgtggacg
     4381 acgtcatccc actgatggca gaaggcaaaa tcctgccgta tctggacatt ccgttgcagc
     4441 acgccagccc gcgcattctc aaactgatga agcgtccggg ttctgtagat cgccaactgg
     4501 cgcgcatcaa acagtggcgc gaaatctgcc cggaactgac cctacgctca acctttattg
     4561 tcggcttccc tggcgagacg gaagaagatt tccagatgct actcgacttc ctgaaagaag
     4621 cacgtctgga tcgcgttggc tgctttaaat acagcccggt tgaaggtgca gacgccaatg
     4681 ccctgcctga ccaggttccg gaagaagtga aagaagaacg ctggaaccgt ttcatgcagc
     4741 tgcagcagca gatttccgcc gagcgcctgc aagagaaagt gggccgtgaa attctggtga
     4801 ttatcgacga agtggacgaa gaaggcgcga ttggtcgcag catggcagat gcaccggaaa
     4861 tcgacggcgc ggtttatctc aatggtgaaa ccaacgttaa gccgggtgat atcctgcgtg
     4921 tgaaagtcga gcacgccgat gagtacgatt tgtggggtag ccgggtttaa ttaattcagg
     4981 ccgggcaaaa tcatttgtcc ggcctgcgaa taatgattac tgccagctaa tctgtgccct
     5041 gccgtttttc ttactcttat acagccgctc atccgccagc attttcaagc gttcgacggt
     5101 agtacattca tttgaatgcg ctacaccgat actggtgctt aaattaatgc ttcccacctg
     5161 gaaatgaacg atttctttta gtatcgatag caactcttcc agcgtgccgc cagtataaat
     5221 caccgcaaat tcgtcacctc cgaagcggta gactttacct ttatctaccg ggatctgtga
     5281 tttaaggatt ttcaccactt tcatcaacac ttcatcgccc agcaaatgac cgaaggtgtc
     5341 gttaatgttt ttgaatttat cgacgtcaaa cagacacaaa taaccgcctt tttccggcag
     5401 cgcttttaaa tcttcatcaa aggattttct gcccattgct ttggtcaggt cgtccaggaa
     5461 cgccgagtta tacatactgt agagtttttt gacatcggca gccgaaaaga aaagcccgcc
     5521 atccagtacg cgttcaaaat ggttattaat gatggcttct ttctccacaa taccacgatt
     5581 aatcactctg cgtaccagat agaggcaact ggcacccaat atcactgcca ccaatgcggc
     5641 aaagaaaata atcaatgtac aaacccgcaa ataaacatct tttttatacg caaatacgta
     5701 caatccggtc ggcaaatagg cgaagtgctc catcgcttcc gaaacggtga agatggcgtt
     5761 tttagcatta tattcatcac cggtcaccgg tttaccgttc ttaaatagag agaacatcag
     5821 gttctcgctg tccatcacca gcgattttga cagtaaagaa aaacgctcca gaccaagcaa
     5881 aaatatcaga ctacgattgt gagttaccgg atagaggtaa tagacgaagg ttttaccgcc
     5941 aaaagagata aaccctgtat ttttgacatg tcgatacttc acaacctgca taatatctgc
     6001 acaataattt ttctcgaact cctttacctg aatacattcg tcactggaaa tcagataagg
     6061 accgacatcg tacaaataag agggggaaac cgagtcggcg taagctgtgc gcaaaccaac
     6121 caacagctgt tcctccgtgg gatttagcgt gttgataaga tcaattgcca cgctacgccg
     6181 atattcagca atatttgcca ccgcaaaagc agtatcaata acggactgtt tcaggctata
     6241 cacccgttcc tttaccattt gagtgtagat cccgcaggca actgctgaaa tcatgataaa
     6301 aatcagcaga ctgactgtaa gtacgaactt attgattctg gacatacgta aactactctt
     6361 ttactaattt tccactttta tcccaagcgg agaatggcat gggtttctgg tacagatacc
     6421 cctgagcctg aacacatcct aatttaatta attcgcgcgc aacgtcttcg ctctctacgc
     6481 cttccgcaat caccgggatc cggttatagc gtgccagatc gaaaataatg cgcacaattt
     6541 ttcccgcagt accgaaaata tcgccggtaa attgcgagtc cacctttatg ctgtctggat
     6601 taatttcata aaaacgtttc aggtttgaca aaccggtgcc aaagtcatca atcgatatcc
     6661 gtaccccttg tttacgcagt tgttcaatat tgagataaaa atgttcatta attcgctcga
     6721 tcacggtttc agtgatctca acacagaggc tgttcgcctt caggccattt tctttcatga
     6781 tggtggtaaa acgctcatga aagtgcggtt cgtttaactg ccagggcgaa acattaatat
     6841 gcagtaagaa atcatccttc agcgacgcac gttgactaaa atgtctgaat taacccattg
     6901 ccgttttaat aatctgataa cccagctcat tgataaaccc gatatcttca gcaatggtaa
     6961 tgaacttcag cggtgaaatg atcccccgct ccgcagaaac ccaacggcac aacgcctccg
     7021 cgctcagaat ggcgcgggtg ttaatatcaa cgatcggttg atagacgagg aatagttcgc
     7081 caatttccat tgcatggcga atattacgcg caatccagat attctcttct atttcgttga
     7141 gtttcgcgtc atcacagaaa ataaactcgg agaacatatg gtcacggata ctggaaatcg
     7201 ccagtttcag tttttctgcg taactccagg cgttttcccc ctgcaaaggt tcacaaatgg
     7261 cagacttatg gatgcgcaga ttgcggtagc caaagctgct gtcaaaatcg tttaccatcg
     7321 ctattttacg ggtaaatgtc tgtacatttt cccgacacag aagtaaatag agatcggcat
     7381 tgaatgtcac gatatcgcag cattgcgcat atttctcggc aattcgctga cacaatttcc
     7441 tgacgatttt attgcgttca tcaacaccaa acaccgcctc cacggcttcc agaccacaca
     7501 gtttgatgat ccccacagcc agatactgat tattgttgga ctcaataatc gccatatttt
     7561 taataaaacc gctttcctga tcgtaagaga tcagtgagag caatttattt gaccgttcaa
     7621 tttcggcatc tttcatttcc gcgatcgaat ttttgatgct gataatctct ttcgcatcgc
     7681 cttcgcgaat tttcagagcc tcgatctgat ccgttgagtc gggcaaaaac gccagcgtat
     7741 taaccagctc ggtcatttca gaaaattgcc gacggaacac acgagtggtc atgaaatata
     7801 ccaccagcaa cacgataata atgaacgcaa acggtagata aaaaagttta tctgatgcgt
     7861 tcaataaatt accgccagga taataaaaat agatttcgaa caatgcatta tttggcactg
     7921 ctgatgtctt tgcaatatac tccgtatcga cagaatcaat aatattataa ttaccttcgc
     7981 tattataaat aatcagtttt tcacgagcga gttgattctt ggcaatgatc tcatgacctt
     8041 gtttcaaaaa tagaacttca tcattagcgc tgatttgatc aagatactcg ttcagatacc
     8101 gccagtttaa atcgacatga attttatcta ccgctaacaa atcgtgatct ttgccgttga
     8161 tacgtagcgt aaaagagata cattttttac ccggacgatc aaagcaattg tatgccggat
     8221 tccagctgtt gttagagtca tctgaagcca ccgaccacgg tcgtttcgat gggttaaaat
     8281 cggcaaaaat aacccgccct gactcgtcga caagcggtcc atttacttgt tcctgatgat
     8341 aaacaacgat tttatcattc gccctccgcg aaaagagata atatttatta tcatcaagta
     8401 ccagtcccac cgagtctaaa aatggcatca tttccatttt cagaatcacg gcgcgttgaa
     8461 tctcctctgt attttttaat aaatacagtg agtcctttaa tgtcagggcc gaacgttcaa
     8521 tatctttaaa gaaatagcca acgtttttct gaataagttc aaagcggcta tcggcacgtt
     8581 ttaccgcgcg ttccgatacc agttgatagt taatcatgaa aaaaagacca ataaatgaca
     8641 gtgctgccaa taaaaataaa attatcagcc ttatcttttc gtataaactc agcatgttaa
     8701 tacatttaac aaaataacta tctgaacgct gtgccgctgc aaaaatagtc ccttgctgcg
     8761 gctttttaac caaattaccc gattctacaa aatcagcaga aagttacaac acttgcttag
     8821 ggcaataatt tcctgtgagg tgattaccct ttcaagcaat attcaaacgt aactatcctt
     8881 taattttcgg atccagcgca tcgcgtaaac catcgcccaa caaattgaac gccagtacgg
     8941 tcagaaaaat atccagggcc ggaaaaacag cgacatgcgg cgcgataacc atatccgctc
     9001 gtgcctcatt gagcattgct ccccactctg gtgtcggcgg ctgcgcaccg aggccgagaa
     9061 atgacaggct ggcggcagag ataatcgagg tgccaatgcg catggtgaaa aacaccacga
     9121 tagaagagac ggtcccaggc aggatatgac gcaataaaat ggtcatatcg ctggcaccaa
     9181 tactgcgtgc tgactcaata aaggtttgct gtttcaacac cagcgtgttg ccgcgcacca
     9241 ggcgggcaaa cgcggggatg gaaaaaatgg cgactgcgat aatcacgtta gcaatgccgc
     9301 ttcccaacac cgcaacaaca gcgatcgcca gtaaaatacc cgggaaggca aacagcacat
     9361 cgcaaatgcg catgatcagc cgatcccacc agccttcata atatccagcc aacaagccca
     9421 gcaacgtccc tatcgccaca ccgataaaca cggcaaacac gcccgccgcc agcgagattt
     9481 gcgcaccaac caggacacgg ctgaaaatgt cgcgccccag tgaatcgacg ccaaaccagt
     9541 gctgcaaaga aggtccgtta ttcagattgt cataatcaaa ataattttcg gcgtcatagg
     9601 gagcgatcca gcgtgcaaaa atggccacca caatcaataa aataacgaat aatgcggcgg
     9661 tcatcgccat atgctgacgg cgaaatcgtc gccagaattc atgccacggt gtacgtacct
     9721 ggtcaggttt gaccagtggc atggcgttta acaccgcctg acgtcgccag ttaaatagtc
     9781 gcatccttac ttgtacctga tagccgggtt aatggcggcg taaagcacat ccaccactaa
     9841 gttgataaga ataaattcca gcgagaaaag cagaatttcc gcctgaatca ccgggtaatc
     9901 gcgcatttct acagagtcaa cgagtaagcg tccaagtccg ggccagttga aaactttctc
     9961 caccacgata gaaccaccga gcaaaaagcc aaactgtaag cccatcatgg tcactaccgg
    10021 gatcatcgcg ttgcgtagcc cgtgtttgag gacaacccag gtttcgctca cccctttcgc
    10081 cctcgcggta cgcatataat cttcgcttaa aacgtcgaca aatgacgcac gggtaaagcg
    10141 cgccatcacg gcggccactg ccgcgccgag cgtcagggag ggtaaaatgt agtgctgcca
    10201 actgtctgct cccacagtag gcagccagcc cagttcaacg gagaatacct gaattaaaaa
    10261 catccccagc gcaaatgccg gaaacgagat ccccgacacc gcaatggtca tactcaatcg
    10321 atccggccaa cgattacgcc agacggcggc gataattccc gccgccatac caaatatcac
    10381 cgcccagacc atactggtta tggtcagcca tagcgttggc ataaagcggc tggcaatctc
    10441 atcggcaacc ggacgacgcg acaccatcga caggccaaaa tccccctgca cagcattgct
    10501 gatatagtgc cagaactggt gatacagcgg ctgatccaac cccagctgct gacgcaccag
    10561 ttctataacc tgcgcatcag cttcgggccc ggcaatcaat cgcgccggat cgccgggcag
    10621 catgtggaca aataaaaaca ccagcaccga gacgataaac agcgtcggaa tcaaccccag
    10681 taatcgtttg ataacgtaat aaagcattcc actccctgcg ttgcttattg caaatccgcg
    10741 tcttcaaagc tgaagccggt atctggcatg atccaaaaac cggtcaggtt tttactgtgt
    10801 gccgacacca gtttttctac caccagcggg atccacggcg attcttgcca gatgatatcc
    10861 tgcgccgcct tatataagcg ggtcttttcc gccggatcat tcgttttcag tgcctgagcc
    10921 aggaagtcat ccacctgttt attgctgtaa aacgcggtat taaacagcgt cggtggccag
    10981 ttttgcgagg caaacagcgg cgatagtgcc cagtcggctt cgccggttga agccgaccag
    11041 ccagtgtaga acatccgcac gccactctct ttttgccctt taccttcaac ttctgccgcc
    11101 cgctgtccgg catccatcgc ggtcacctgg gctttaatcc cgacctgcgc taactgctgc
    11161 tgggtaaatt gcagcacttt ctgcgcggtg ctgtggttat gtgacgacca cagcgtggta
    11221 ctgaaaccgt tgggatatcc cgcctctttc agtaattcgc gcgctttcac tggatcgtaa
    11281 ggccacggtt tataactttg cgcgtaggcg atacttggcg gtaccacacc agtagctgga
    11341 gttgcatagc ccgcaaaggc aactttcacc agcgccggac ggttaatggc gtaattcagc
    11401 gcctcacgga ccttcgggtt atcgaacggc ttttgcgtca cgttcatact gatataacgc
    11461 tgcataattg acggactggc catcaactcg atatttttgt ttttctccag cagtgtggcc
    11521 tgctcgtagg gaatggggaa agcaaactgc gcttcaccgg tttgcagcat tgccgcgcgg
    11581 gtgttgttat ccgccaccgg acgccaggtt atgctgtcca gtttgggcaa tcctggctgc
    11641 cagtaacccg cgaatttttt caccttcaca aaatcggtct gattccaggt atccagttca
    11701 tacggtccgg ttcccaccgg atgaaaacca atctccttgc catatttttc cagcgctgtc
    11761 ggtgaaatca tcgcggtcgc cggatgggca agaatattaa taaacgctga gaacggctgt
    11821 ttgagggtaa tctttaccgt tgtcggatcg atcgcctccg ttttggcaat attcttatac
    11881 aggttatagc gtttaagatg attcgccgga tcgctggccc ggtccagatt cgcttttacc
    11941 gccactgcgt tgaaatcggt gccatcctgg aatttaattc cttcccgcaa tttcacggtg
    12001 taagtaatgc cgtcatcgga aacggtataa ctctccgcca gcacgttttt cagtttcatc
    12061 tctttatcca gaccgaacag cccctggtaa aacgatttcg ctacggcctg agataacgtg
    12121 tcattcgcgt catacggatc gagcgtggtg aaattcgatc ctaccgccac caccacatct
    12181 ttggcagcga atgcacaaga tgccatcaac gctgtcgcaa tgcccagcgc cactaaccca
    12241 ctacggtgta cagctcttgc cattttattc tccgcctgaa tgttatctac gcatgaatgc
    12301 gtattctgat tgtggttgtg cgacgtaatg ccccggcccg acgcattgca acgagacggc
    12361 tgccacctct tcgccacgca gatgaatatt gctgggaaga tcgtccgaca gcagtacacg
    12421 ctgcggtcgt tgtcgggacg gttcagcgac cggaactgcc gccagtaatt tacgcgtata
    12481 aggatgctgc gggttttcga agaccgcgca ccgtgaacca atttcaacaa t
//
LOCUS       DADLTY010000122        12515 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.122, whole genome shotgun sequence.
ACCESSION   DADLTY010000122 DADLTY010000000
VERSION     DADLTY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.122"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..401
                     /locus_tag="JN334_002917"
     CDS             <1..401
                     /locus_tag="JN334_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7438991.1"
                     /translation="FARVNKAGTPVAGLIIVGILMTIFQLSSISPNATKEFGLVSSVS
                     VIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTTIAFLYCIWAVVGSGAKEVMWSFV
                     TLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            505..2148
                     /gene="eptA"
                     /locus_tag="JN334_002918"
     CDS             505..2148
                     /gene="eptA"
                     /locus_tag="JN334_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HAY7438992.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            2145..2813
                     /gene="basR"
                     /locus_tag="JN334_002919"
     CDS             2145..2813
                     /gene="basR"
                     /locus_tag="JN334_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HAY7438993.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            2814..3914
                     /gene="pmrB"
                     /locus_tag="JN334_002920"
     CDS             2814..3914
                     /gene="pmrB"
                     /locus_tag="JN334_002920"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HAY7438994.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(3890..3979)
                     /gene="pmrR"
                     /locus_tag="JN334_002921"
     CDS             complement(3890..3979)
                     /gene="pmrR"
                     /locus_tag="JN334_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HAY7438995.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(4091..5593)
                     /gene="proP"
                     /locus_tag="JN334_002922"
     CDS             complement(4091..5593)
                     /gene="proP"
                     /locus_tag="JN334_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HAY7438996.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(5857..6735)
                     /locus_tag="JN334_002923"
     CDS             complement(5857..6735)
                     /locus_tag="JN334_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7438997.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(6732..8960)
                     /gene="crfC"
                     /locus_tag="JN334_002924"
     CDS             complement(6732..8960)
                     /gene="crfC"
                     /locus_tag="JN334_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HAY7438998.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            9363..9698
                     /locus_tag="JN334_002925"
     CDS             9363..9698
                     /locus_tag="JN334_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HAY7438999.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            9856..10299
                     /gene="yjdN"
                     /locus_tag="JN334_002926"
     CDS             9856..10299
                     /gene="yjdN"
                     /locus_tag="JN334_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HAY7439000.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            10432..11220
                     /gene="phnC"
                     /locus_tag="JN334_002927"
     CDS             10432..11220
                     /gene="phnC"
                     /locus_tag="JN334_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAY7439001.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            11245..12261
                     /gene="phnD"
                     /locus_tag="JN334_002928"
     CDS             11245..12261
                     /gene="phnD"
                     /locus_tag="JN334_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7439002.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            12367..>12515
                     /locus_tag="JN334_002929"
     CDS             12367..>12515
                     /locus_tag="JN334_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HAY7439003.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         3190 a         3350 c         3098 g         2877 t
ORIGIN      
        1 tttttgcccg tgtaaataaa gcgggtacgc cagtggcggg gctgattatc gtcggtattt
       61 tgatgaccat cttccagctc agcagcattt caccaaacgc gaccaaagag ttcggtctgg
      121 tttcttccgt ctcggtcatc tttacactgg tgccatatct ttacacctgt gcggcgttac
      181 tgctgctcgg ccacggtcac tttggtaaag cacgcccggc atatctggct gttactacca
      241 ttgccttcct ctactgcatt tgggccgtgg tggggtccgg ggcgaaagag gttatgtggt
      301 catttgtcac cctgatggtc atcaccgcca tgtatgcgct gaattacaac cggctacata
      361 aaaacccgta tcccttagat gcaccaataa gcaaagatta attcccctta atccagcaaa
      421 cataaaagcc aaccttaaga acttaaggtt ggcttaattt tgctttgcga gcatatgcgc
      481 actttgttcg atggaaacac cgtgatgttg aagcgcctac taaaaagacc ctctttgaat
      541 ttactcgcct ggctattgtt ggccgctttt tatatctcta tctgcctgaa tattgccttt
      601 tttaaacagg tgttgcaggc gctgccgctg gattcgctgc ataacgtact ggttttcttg
      661 tcgatgccgg ttgtcgcttt cagcgtgatt aatattgtcc tgacactaag ctctttctta
      721 tggcttaatc gaccactggc ctgcctgttt attctgattg gcgcggctgc acaatatttc
      781 ataatgactt acggcatcgt catcgaccgc tcgatgattg ccaatattat tgataccact
      841 ccagcagaaa gttatgcgct gatgacaccg caaatgttat taacgctggg attcagcggc
      901 gtgcttgctg agctgattgc ctgctggcta aaaatcaaac ctgccacctc gcgtctgcgc
      961 agtgttcttt tccgtggagc caatattctg gtttctgtac tgctgatttt gctggtcgcc
     1021 gcactgtttt ataaagacta cgcctcgttg ttccgcaata acaaagagct ggtgaaatcc
     1081 ttaagcccct ctaacagcat tgttgccagc tggtcatggt actcccatca gcgactggca
     1141 aatctgccgc tggtgcgaat tggtgaagac gcgcaccgca acccgttaat gcagaacgaa
     1201 aaacgtaaaa atttgaccat cctgattgtc ggcgaaacct cgcgggcgga gaacttctcc
     1261 ctcaacggct acccgcgtga aactaacccg cggctggcga aagataacgt ggtctatttc
     1321 cctaataccg catcttgcgg cacggcaacg gcagtttcag taccgtgcat gttctcggat
     1381 atgccgcgtg agcactacaa agaagagctg gcacagcacc aggaaggcgt gctggatatc
     1441 attcagcgag cgggcatcaa cgtgctgtgg aatgacaacg atggcggctg taaaggtgcc
     1501 tgcgaccgcg tgcctcacca gaacgtcacc gcgctgaatc tgcctgatca gtgcatcaac
     1561 ggcgaatgct atgacgaagt gctgttccac gggctggaag agtacatcaa taacctgcag
     1621 ggtgatggcg tgattgtctt acacaccatc ggcagccacg gtccgaccta ttacaaccgc
     1681 tatccgccgc agttcaggaa atttacccca acctgcgaca ccaatgagat ccagacctgt
     1741 accaaagagc aactggtgaa cacttacgac aacacgctgg tttacgtcga ctatattgtt
     1801 gataaagcga ttaatctgct taaagaacat caggataaat ttaccaccag cctggtttat
     1861 ctttctgacc acggtgaatc gttaggtgaa aatggcatct atctgcacgg tctgccttat
     1921 gccatcgccc cggatagcca aaaacaggtg ccaatgctgc tgtggctgtc ggaggattat
     1981 caaaagcggt atcaggttga ccagaactgc ctgcaaaaac aggcgcaaac gcaacactat
     2041 tcacaagaca atttattctc cacgctattg ggattaactg gcgttgagac gaagtattac
     2101 caggccgcgg atgatattct gcaaacttgc aggagagtga gtaaatgaaa attctgattg
     2161 ttgaagacga tacgctgtta ttgcagggac tgattctggc ggcgcaaacc gaaggctacg
     2221 cgtgcgatgg cgtgacaacc gcgcggatgg cggaacaaag ccttgaggac ggtcattaca
     2281 gcctggtggt actggattta gggttacccg acgaagatgg actgcatttt ctcgcccgta
     2341 tccggcagaa aaaatacacc ctgccggtac tgatcctcac cgctcgcgat acgctgaccg
     2401 acaaaatcgc cgggctggat gtcggtgccg atgactatct ggtgaagcct tttgcgctgg
     2461 aagagttaca cgcccgtatc cgcgccctgc tacgacgcca taataatcag ggcgaaagtg
     2521 agctgattgt tggcaatctg acgctgaata tgggtcgccg tcaggtatgg atgggcggtg
     2581 aagagttgat tctgacgccc aaagaatatg ctctgctgtc acggttaatg ctcaaagcag
     2641 gcagtccggt acatcgggaa attctctaca acgacatcta taactgggac aatgaaccct
     2701 cgaccaacac cctggaagtg catatccaca atctgcgcga caaagtgggc aaagcccgta
     2761 tccgcaccgt gcgcggcttt ggctatatgc tggtcgcgaa tgaggaaaac taattgaatc
     2821 tgatgcgttt tctgcgccga cctatatcgc tgcgccaacg gctgatattg accatcgggg
     2881 ccattttgtt ggtgtttgag ctgatcagcg tcttctggct atggcatgaa agtaccgagc
     2941 agattcagct gtttgagcag gcgctacgcg acaatcgcaa caacgatcgc cacatcatgc
     3001 gtgagatccg cgaggcggtc gccagcctga ttgtccccgg cgtctttatg gtcagcctga
     3061 cgctatttat ctgctatcag gcggtacgcc gcatcacccg cccgctggcg gagctgcaaa
     3121 aagagctgga agcgcgcacc gccgacaact taacgcccat tgccattcac agcgccaccc
     3181 tcgaaatcga agcggtggtt tcggcgttga acgatctggt cagtcgcctg accagcacgc
     3241 tggataacga aaggttgttt accgctgacg tcgcgcacga actgcgaacg ccactggcgg
     3301 gggtgcgttt gcatctggaa ctgctggcga aaacgcatca cattgatgta gcaccgttag
     3361 tggcacggct tgatcagatg atggagagcg tctcccagct gctgcaactg gcgcgtgccg
     3421 gacagtcatt ttcttccggt aattatcaac atgtaaaact gctggaagat gtcattctcc
     3481 cctcgtatga cgaactcagt accatgctcg accagcgaca gcaaaccctg ctattgccag
     3541 agagcgccgc cgacatcacc gttcagggtg atgcaaccct gctgcggatg ttattgcgaa
     3601 acctggtaga aaacgcccat cgttacagcc cgcaaggcag caacattatg attaagctgc
     3661 aagaagatgg cggagcggtc atggcagttg aagatgaagg accaggtatt gatgagagta
     3721 aatgcgggga gttgagtaaa gcgtttgtac gtatggacag ccgttatggc gggattggtc
     3781 tggggttaag tattgtcagc cgcattacac agttgcatca cgggcagttt ttcctgcaaa
     3841 accggcaaga gacttccggc acgcgggcct gggtacggct gaagaaagat cagaacgtgg
     3901 caaaccagat ataaagaaag ctgctgacca ccagcacgct gaacacggta gttaaacttt
     3961 cataaacacg gtttttcatt gcactctcct ccgttaacct ggaggagagt atgcgcgtca
     4021 gagattaagt gaaccttaag agttcaattg gcctgataag acagcgtcac atcaggccat
     4081 ccgtttcagc ttattcatca attcgcggat gttgctgcac caggcgggta cgtttcgcct
     4141 gcaaatcggc aatctcgtgg tcaatattat cgattttctg ctcgatatta tcgtaatgct
     4201 cgacgagaat ttccttcgct tcctgtatat ctgacgccgc cggtgtcgca cctttcaacg
     4261 gacgatttgc cgtctctttc atggttacgc cggtgattaa accaatcacc gccactacca
     4321 tcaggtaata ggcaggcatc atcagattct gcgagctttc gaccagccag gccgccagtg
     4381 ttggcgtcag accggcaacc agcaccgaaa tattaaatgc cgccgccagc gcgctgtaac
     4441 ggatatgcgt cgggaacatc gctggcaagg tagaagccat aacgcccata aagcagttaa
     4501 ggatcaccgc cagcatcagt aacccggcaa aaatcaggcc gatgacgtta ctgttaatca
     4561 gaataaacgc cgggatcgcc aacacaaaca gtgcaacact accaagtagc acaaacggac
     4621 gacggccaaa acggtcactc agcaagccca tcaccggctg gacaaacagc ataccgatca
     4681 taatggcgat aataatcagc accccgtggt cttcggagta atgcaggtta tgcgacaaat
     4741 aactcggcat ataggtcagc aacatgtagt aagtcacgtt ggtggcaatt accagaccaa
     4801 tacatgtcaa caggctgcgc cagtatttag tggcaatctc tttaaacgag actttcgggc
     4861 catcctgcaa accttcgcgg tcgccctgtt ccagtttatc aacatgctgc tggaacgccg
     4921 gagtttcttc cagcgcatgg cgcaggtaaa gcccgataat ccctaacggc agagcaataa
     4981 agaacggaat acgccagccc cagtcgagga agttcgcttc gccgacaatg gtcgaaatta
     5041 acaccaccac gcccgcaccc agcacaaacc cggcaataga accgaagtcc agccagctgc
     5101 ccataaagcc acgtttacgg tcaggggagt attccgcaac aaatatcgac gccccggtat
     5161 attcaccacc gaccgagaaa ccttgtgcca tcttacagat caacagcaga atcggtgccc
     5221 aaataccaat cgtgtcgtag gacggtatta agccaataca gaacgtactg atcgacataa
     5281 tcacaatagt gatagcgagg atcttctggc gaccatattt atcgcccaac ataccaaaga
     5341 agagtccgcc aagcggtcga atcagaaagg gaacggagaa agtggcaagt gcagcaacca
     5401 tctgcacgct ggggtcagcc cccgggaaaa aaactttacc taatgcgtaa gcaacaaaac
     5461 cataaacacc aaaatcaaac cattccattg cattacccag tgatgctgcg gtaatggctt
     5521 tacgcagttt accgtcatca ataatggtga catcacgaag ggtaatcggt tttacttttt
     5581 tccttttcag catagctttc ctcgcagagt tggcccattt ccgcaaacgc cagaaaaatc
     5641 agcgcgacgg ccgcaaaagg ggagcgggaa ttgcaggatg caatctctgt aacaagcgta
     5701 gcgggtttgc ttacaccctc cggttaagga atgtacaatc aaaccagttg acgcctgaat
     5761 tgggcagtta atgacctttt taccctacca gtgtttaaat ttgtgatcaa cttcacacat
     5821 ggatttcgtt taggactcat tgatgtaact gattatttac gccaaattta cagagaagat
     5881 ttcctcgacc attgccccca ccaaacgctg cgctgaacag agtcgtggca tccccagcgc
     5941 gacagtttgc cagcgttggg tcactgacca gcaaacgggt tgtttatctg ccgtggtcca
     6001 ctttcccagc caacttaaaa aatcagcatg atccccgccc gtcggaacgg tcgaagaggc
     6061 aaaccactga tggtaatagt gacgcgtagt cggtgccgca ttgatactct ctgccagatg
     6121 ttgtgtcgct ttctcgcgca aatcacccag caacaacacc accgcttgcg ggcttcccag
     6181 acggcaggca tcgccaaatg caccccagcg caactcctcc agaacgataa aacagcgccc
     6241 aggcaacgac caggcgttat acgccccagc acgccagcgg gtaaaaattt gctcgctctg
     6301 atcacctgcc tgcaccagta ggcctcgttg cgttaacgcc ttttccttcc agcgacaacg
     6361 ggctgaaaac cactcataca aggcggcaat ttgctggggg agctccggga aagcagagtg
     6421 ctggcggtga tccatttttt gcagcagcgc cagatgctgc gcgatctgcg tcagcgccag
     6481 atgaccggga tccagcatcg acgccagttt ttccaccatt gtcagtcgca ggctaagcgg
     6541 cgcatttaac atgccgccgt tcgtccatgt ctgaagctgc acacgcgaaa gaagttcctg
     6601 catcagtcgt tcacgaaact gctgctgatg ggcttgagga tggcgggcat catcgccctg
     6661 cgccagatcc actaaaaagc gcggatgaac cgactccagc acgcgaccgg ggccgtccag
     6721 taacgtcttg gtcaatatcg ttctgccgtg aaaagtgttt gaatatcatc gcgtaacagc
     6781 tgagcgtcgg tgtaaatcca gctgtgagtc atcacagtct gctgcaattg ctgctgcatc
     6841 agcctgacca ccgattcatt ttgttgacgc agagccagac tttcgcgtaa acgcgtctgt
     6901 aattctgtca aacatgaagc gaactcagcg aaaaaagtat tcatgcctgc cgtaacagat
     6961 tcatcgacct gctctgccag aactttagcc atttgttggc aatagagatc gacttctgcg
     7021 cttaatgctc gttgcaacac actgtaatca accgtttctg tcggggattt ctcatttccc
     7081 cgtccccagt cgggctgatt caaccagcgc gaaaaagtct cacgcacaac gcctaaacgc
     7141 gtgctctgct cgtccgttgc ttcctggcgc gaaatgactt cactgaacag ctggcgagtg
     7201 ttgaagtggg gaactacgcc gtgaaaaaca ggaaaatgaa acccaggacg aaaccctgac
     7261 tcgctcaatt ccattttgac ttgttgctca atggggcgaa taacatcggt taacacccgg
     7321 caaagggtgg attccatctc ggcaaaacgc agcgtaaagt cgcgactgat ggtgttctgc
     7381 gccgtctgta acagtgtctc acagcgggta cgaatctcgc ttaacggctc cgaatcatcc
     7441 tgaaacaagg cggctaactg cgcattcacc gcatcttgct gttgacgcag aaagtggttg
     7501 gcggaggtca gggccagctc gatttcatgt ttaatctcgc cgctcacctg cgcctgattg
     7561 agttgcaata gctgcaaact ttcttcgacc tgatggatat tttgccgcaa ttgttcacaa
     7621 gcgacgttta acccgtgcgc acgaaaatcc aggtattccc gcgcctgctg cgcgtaattc
     7681 aacagtttat gcgcagcaga tcgcagagca tacaatgagg cgttagcgta agcggcatga
     7741 agcaacgcct gaattggctg ggcgaacagc gaatcttccc acaactgatc ggcagcatga
     7801 cgaatatgtt cgaggtccgc cagatcggca tgacgccagc gcctgccgag cgcggcatgg
     7861 gcaaaatctt ccacccagcg ttgttgctct ggcgctggta acttaccgtt gttggctaac
     7921 tcatagcgcg cccgattcgc caggtagccc cacatcgacg acaccgggaa tatctgctgt
     7981 ggcgtaatac agcctttcat cagcgtcccg gaaatcagtg cccgcacctg gtcggcgtcg
     8041 tcactgttac gatcctgttg atcgaactta ttgaccagca catacagcgg caccgattgc
     8101 cccaccgcca aaatcgcctc acggacctct tcatcggaga tcgatttcag ttgcgtataa
     8161 tccagcaccg ccagtaccgc cgaggcgcgt gccagctgct ggttaagcat tttttgcaga
     8221 tgcggttgcc cggcttcatt tggcccgggg gtatccagta acgtcaactg accgggataa
     8281 ctttccagcc ccgccagatg gacaaactcc acttcaatca cgggaatatg ctcaatggcg
     8341 gcgtaagcag aaaaaggaaa atcgacgtcc agcgccttcg ccagtcgcac taaatcattc
     8401 aaacttttca gacaatgaaa aataggctgg gcacccagat aatatttttc gaaagcgacg
     8461 ccattttcga tccgctgcat aagcgcacgc atatctttat ctatttccag cacatcggtc
     8521 agatgcttaa tatcgcaatc acgcaggcgc tgttgtaatt gttgaattaa acaatcgatt
     8581 ggcgcgacat gtgaaaaatg cagtaccggt tccttttgcc cgggcgtatg gcgaataagc
     8641 gtcggcagcg cagtcattgg gcgattacga ttaggcagaa cctccgtacc aacaatggca
     8701 ttaatggtgg ttgatttccc tgctttcatg gtaccgacaa ttgccagcac catttccagt
     8761 cgggaaattt tacacaactc attattcagc atcgcgtgac gttcggcgat attaggctga
     8821 ctccagggta aagccagttg tggcgcgtcg tctccgggta cagagagagg cattttttcc
     8881 agtaactgca actgttggcg agaaagctgt aacaggcgtt cagcctcctg acttaactca
     8941 tacagggtct gtgtgtacat agaaaattct tccttaaagc aaattttgtt acttttattt
     9001 agccagattg tttttgagtt ctgttttcgg cttttataat tactgcaaga aataatttta
     9061 tatttagtgt gttgtttttt tatcagaata aataacgtct tctgatgcgt ttaaaacgtc
     9121 agaaagataa aaatatcatg tgaataaaaa aaagaacaag tagagcatta acattatctt
     9181 aaataataaa tagaggcaaa aagattattt tctttttgcg tttcctttca aatgaaaacg
     9241 atcgtcgtct aaaatcagca gtacccccga caaactcagg gattttgtgt ataattgcgg
     9301 cctttttcgg caatctgccg ttttttggcg cttttgccct gctgactttt gaggaaatcc
     9361 acatgtcatt accacactgc ccaaaatgca actccgaata cacttacgaa gataacggca
     9421 tgtacatctg cccggaatgt gcctacgaat ggaacgacgc agaacctgca caggaaagcg
     9481 acgagctgat cgttaaagat gctaacggca atctgctggc tgacggcgac agcgttacca
     9541 tcattaaaga tctgaaggtg aaaggtagct cttcgatgct gaaaattggc accaaagtga
     9601 aaaacatccg cctggttgaa ggcgaccata acatcgattg caaaatcgac ggttttggtc
     9661 cgatgaaact gaaatctgag tttgtgaaaa agaactgatt gtattgtgat cggtaagccg
     9721 gataaggcgc tcgcgccgca tccggcaacg atgccgactg cctgatgcga cgcttgcgcg
     9781 tcttatcagg cagcctctca tctgtataaa tttcgaacta cacttaactg gcttctctta
     9841 actgaggtca ccatcatgcc gttaagtccc tacctctctt ttgccggtaa ctgttccgac
     9901 gcgattgcct attatcaacg tacgttgggc gcggaactgc tctataaaat cagcttcggc
     9961 gaaatgccga aatcagcgca ggacagcgcc gagaactgcc cttccggaat gcaatttccc
    10021 gataccgcca tcgctcatgc caacgtgcgc attgccggaa gcgacatcat gatgagcgat
    10081 gctataccgt caggaaaagc cagctactcc ggctttacgc tggtgctcga ttcgcaacag
    10141 gtcgaagaag gaaaacgctg gtttgataac cttgccgcta acggaaaaat cgaaatggcc
    10201 tggcaggaaa ctttctgggc gcatggcttt ggcaaagtca ccgataaatt tggcgtaccg
    10261 tggatgatta atgtcgtcaa acaacaacca acgcaataac ccgccgggag gcccgccctc
    10321 ccgcactgtc atcgaattcc cgttaactct tcatctgtta gtcactttta attaaccaaa
    10381 tcgtcacaat aatccgccac gatggagcca cttttttagg gaggctgcat catgcaaacg
    10441 attatccgtg tcgagaagct cgccaaaacc ttcaatcagc atcaagcgct gcatgcggtt
    10501 gatctgaaca ttcatcacag tgaaatggtg gctctgcttg ggccgtcggg ttccggcaaa
    10561 tccacccttt tacgtcattt aagcggtttg attaccggcg ataaatccgc cggtagccat
    10621 atcgagctac tgggccgcac tgtccagcgc gaaggccgcc tggcccgcga tatccgcaaa
    10681 agccgcgcca acaccggcta catctttcaa caattcaacc tggtgaaccg cctgagcgta
    10741 ctggagaacg tgctgattgg cgcgctcggc agcacgccgt tctggcgcac ctgttttagc
    10801 tggttcaccg gcgagcagaa acagcgcgcg ttacaggcgc tgacccgcgt tggcatggtg
    10861 cattttgccc atcagcgcgt ttccaccctc tccggcggcc agcagcaacg tgtggcgatt
    10921 gcccgtgcgc tgatgcagca ggcgaaagtg attctggccg atgaacccat cgcctcgctg
    10981 gacccggaat cagcgcgcat cgtgatggac accctgcgcg acatcaacca gaacgacggc
    11041 atcaccgtgg tcgtcacgct gcatcaggtg gattacgccc tgcgctactg cgaacgcatc
    11101 gtcgccctgc gtcaggggca cgtcttctac gacggcagca gccaactgtt tgataacgaa
    11161 cgttttgacc atctctaccg cagcattaac cgcatcgaag agaacgcgaa agctgcctga
    11221 tatccccatc attgaggaaa acgaatgaac gctaagataa ttgcctcgct ggccttcacc
    11281 agcatgttca gcctcagcac cctgttaacc ccggcacacg ccgaagagca ggaaaaggcg
    11341 ctgaatttcg gcattatttc aacggaatca cagcaaaacc tgaaaccgca atggacgccg
    11401 tttttacagg atatggagaa gaaactgggc gtgaaggtca acgccttctt tgccccggac
    11461 tacgcgggca ttatccaggg gatgcgcttc aataaagtgg atattgcctg gtacggcaac
    11521 ctgtcggcga tggaagcggt ggatcgcgcc aatggccagg tcttcgccca gacggtcgcg
    11581 gcggatggat cgccgggtta ctggagcgtg ttgatcgtca acaaagacag cccgatcaac
    11641 aacctgaacg atctgctggc gaagcggaaa gatctcacct tcggcaatgg cgatcctaac
    11701 tccacctctg gcttcctcgt ccccggttac tacgtcttcg ctaaaaacaa tatctccgcc
    11761 agcgacttca agcgcaccgt caacgccggg catgaaacca acgcgctggc cgtcgccaac
    11821 aagcaagtgg atgttgccac caacaacacc gaaaacctcg acaagctgaa aacctccgcg
    11881 ccggagaagc ttaaagagct gaaggtgatc tggaaatcgc cgctgatcct aggcgatccg
    11941 atcgtctggc gcaagaatct gtccgaaact accaaagaca agatctacga cttctttatg
    12001 aactacggaa aaacgccgga agagaaagcg gtgctggaac gcctgggctg ggcgccgttc
    12061 cgcgcttcca gcgacctgca actggtgccg attcgccagc tcgcactgtt taaagagata
    12121 caaagcgtga aaggcaataa agggctgaat gagcaggaca agctggcgaa aaccaccgag
    12181 attcaggcgc agctggatga cctggaccgc ctgaacaacg cgttaagcgc gatgagttcg
    12241 gtgagtaaag cggtgcagta aatcgtaggt cggataagac gccccggcgt cgcatccgac
    12301 aatgtgtagg cgttgatgcc ggatgcggtg caagcacctt atccggccta cagaccggag
    12361 ccaaacatgc aaaccatcac catcgcccca cccaagcgta gctggttctc gcttctgagc
    12421 tgggccgttg ttctcgacgt gctggtcgtc tcgtggcagg gcgcggaaat ggccccgctt
    12481 acgctgatta aagacggcgg caacatggca acctt
//
LOCUS       DADLTY010000123        12501 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.123, whole genome shotgun sequence.
ACCESSION   DADLTY010000123 DADLTY010000000
VERSION     DADLTY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.123"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..726)
                     /locus_tag="JN334_002930"
     CDS             complement(<1..726)
                     /locus_tag="JN334_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="with CusA, CusC and CusF is part of a cation efflux
                     system that mediates resistance to copper and silver;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAY7439004.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFDQAEPHAEKMPTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPTNVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRHLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKEEGRL"
     gene            complement(742..1074)
                     /gene="cusF"
                     /locus_tag="JN334_002931"
     CDS             complement(742..1074)
                     /gene="cusF"
                     /locus_tag="JN334_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HAY7439005.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1098..2480)
                     /gene="cusC"
                     /locus_tag="JN334_002932"
     CDS             complement(1098..2480)
                     /gene="cusC"
                     /locus_tag="JN334_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HAY7439006.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            2637..3320
                     /gene="cusR"
                     /locus_tag="JN334_002933"
     CDS             2637..3320
                     /gene="cusR"
                     /locus_tag="JN334_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HAY7439007.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3310..4752
                     /gene="cusS"
                     /locus_tag="JN334_002934"
     CDS             3310..4752
                     /gene="cusS"
                     /locus_tag="JN334_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HAY7439008.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            4902..7144
                     /gene="nrfB"
                     /locus_tag="JN334_002935"
                     /pseudo
     CDS             4902..7144
                     /gene="nrfB"
                     /locus_tag="JN334_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            7131..9275
                     /locus_tag="JN334_002936"
                     /pseudo
     CDS             7131..9275
                     /locus_tag="JN334_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741327.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage receptor"
     gene            9392..10099
                     /locus_tag="JN334_002937"
     CDS             9392..10099
                     /locus_tag="JN334_002937"
                     /inference="COORDINATES: protein motif:HMM:NF024679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage N4 adsorption protein A"
                     /protein_id="HAY7439009.1"
                     /translation="MSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVYS
                     RVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRAS
                     ASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQTI
                     EPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAIN
                     QRNGERNNAFLTIGVHW"
     gene            10100..10990
                     /locus_tag="JN334_002938"
     CDS             10100..10990
                     /locus_tag="JN334_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HAY7439010.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11173..11934
                     /locus_tag="JN334_002939"
     CDS             11173..11934
                     /locus_tag="JN334_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7439011.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(11980..12056)
                     /locus_tag="JN334_002940"
     tRNA            complement(11980..12056)
                     /locus_tag="JN334_002940"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12020..12022),aa:Arg,seq:tct)
     gene            12299..>12501
                     /locus_tag="JN334_002941"
     CDS             12299..>12501
                     /locus_tag="JN334_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAY7439012.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3030 a         3267 c         3186 g         3018 t
ORIGIN      
        1 caacctacct tcctctttcg cgaccacgtt atctttggcg atattcattc ccgcgcgcag
       61 atcaaacgcg gtgatcacgc catcaatggg cgctttgagc gtaaagcgag tctggatttt
      121 ttgcgtggcg atcaggtggc gaatatccgc ctccggcatt cccgccagtc gcagccgctc
      181 aagaatgcct tcagtctggg tcgccgtacc gccggtttcg cgcaacagta aatactcact
      241 ctgcgcttcc acccagtcgg gaatggtcag gtcgagaagc ggtgtgccct tttgtacttt
      301 atcgcccacg gtaagcggat acaccttgtc gataaacccg gcagcgcgag cctgcacaat
      361 ggcatactga tactcgttgt aactgacatt cgtcgggaaa ctctgggcaa aagtcagcgg
      421 cccgcgcgtg acggtagccg ttttcacccc cagattctgc gtctgagtcg ggtcaatgcg
      481 cacaccagac gcagaactct cttcatcggc atatttcggc accagatcca tatccataaa
      541 cggcgattta cctggtttat cgaaccgcgt attgggatac atcgggtcgt accagaataa
      601 gattttacgt tctgcggtcg gcattttttc tgcgtgcggt tccgcctggt caaaccaggt
      661 aaaacctgcc gctgaaataa taccgcccgc gatcatgctg ccgataataa gcgcgatttt
      721 tttcatctca ttaaacctgg gttactggct gactttaata tcctgtaata aagaaaggtt
      781 gccctgctgg acaaaattaa acgccacttt gtcgccggtt ttaatttcac tcattttcgt
      841 ctgcggggtg atggtaaagc gcatggtcat ctccggccag ttgacggcag caatcggatc
      901 gtgatggatg gtgatttttt tgctttccag atcaacacct tttaccacac cggtggcact
      961 aatgacctgt ggttgtgctt cgctcatggt ttcatgatga tgttcgttag cctgggcatt
     1021 aaagccaata acggtaaaca gactgaacat tgcgacttgc agtgcttttt tcatttattc
     1081 tctcctggag ttaaaaatta ttgctgccaa ccgcccccta aggcggtata caaataaatt
     1141 tcgttaacct gacgggcata attcaggtca agtaaagttt gtcgggttgc aaataaagaa
     1201 cgctcggcat ccagcacttc cagataactt accgcgccgt gctgatataa tgcccgcgcc
     1261 cgttgcaggg taatttgcag cgacgccaga taacgctgct gggcgctgat ttgatcgtcc
     1321 aggctttgac gtaatgccag cgcatctgcc acctctttaa aggcgttctg gattttctgt
     1381 tcataattca ccaccgactg ctgctggcga atttcggcga tatccagatt ggcctggttg
     1441 cgtccggcat taaaaatggg gatctcaatt ttaggaataa aattccacat cccgctgcta
     1501 gcgttaaata atgacgacag atcgctgctg gcggttgata ttccgctggt caggctgata
     1561 gacgggaaaa atgccgcacg cgcagcacca atattagcat tagccgccat taacgcgtgt
     1621 tcagcttcca taatatccgg gcgctgcaat aagatttgcg acgacaagcc cgccggtaat
     1681 ttaacgcttt gcaggctgcc gctgtttact gtctgcgctt gcggtagctt gccgtagctt
     1741 cccaataaca gttgcaatgc attattcgcc tgcgccagtt ccccctgacg tttagcgatg
     1801 tcactgcggg tactttctat caccccgcga gcctgttcca gcgccagaac attgctgcta
     1861 ccggtcaaca gttgtttttc gacaaacgca tatgactgct gataattacg cagcgtttct
     1921 tcggctattt gcaattgcgc atacgccagc tgctgattga aatagctttg cgcgacatta
     1981 gaaaccagca aaatatgcac cgcgcgctgg gcttcctcag tggctaaata attttggcgc
     2041 tcggcttcgc tcatgttctt taagcgaccg aaaaaatcga gatcaaagct ggcgttaagg
     2101 ccagtcgaga actcccgcgt cgtggctgaa tcgcctttaa gattgccgct ccagctgccg
     2161 ctgccctcgc cattgagctg tgggtagcgg tcggcatcgg tcagacgata ttgcgcccgt
     2221 gcctcctgca ctttcagcgt cgccatgcgc aaatcccggt tattcaccag cgcctcgcta
     2281 atcagcgtct tcacctgatt atcaacaaaa aaggtgcgcc agcccacgtt ctggtagtta
     2341 tccgctgcgt taaccaggcc gttctggctg agtgagaact gctgcggtac gggcattacc
     2401 ggacgctgat aatccggtgc cagtgaacaa ccagttagcg caagggccac acaaaatggc
     2461 agaagtttac aaggagacat aggctcataa tttctggtga ttttatgtcg ccaactttac
     2521 tcgccaggct ctgattttcc ggtgacagga aaatgacaaa attgtcattt tgccaataag
     2581 cgattgccat ctgatcccgc tactctagaa ttgcccgggc aacatgcgga ggaaatatga
     2641 aactgttgat tgtcgaagat gaaaagaaaa ccggagaata cttgaccaaa gggttaaccg
     2701 aagccggttt tgtggtcgat ttggccgaca acgggctgaa tggctaccat ctggcgatga
     2761 ccggtgatta tgacctgata atcctcgata ttatgctgcc agacgtgaac ggctgggata
     2821 tcgtgcgcat gttgcgctcc gccaataaag ggatgccgat tctgttgctt accgcgcttg
     2881 gcaccattga acatcgcgtc aaggggctgg agttgggggc agatgactac ctggtgaaac
     2941 cgttcgcttt tgctgaactg ctggcgcggg tgcgcactct cctgcggcgc ggggcggcgg
     3001 tgattatcga aagtcagttt caggttgccg atctgatggt cgatctcgtc agccgcaaag
     3061 tcacccgcag cggcacgcgc atcactctga ccagtaaaga atttactctg ctggagtttt
     3121 ttctccgcca tcagggcgaa gtgctgcccc gctcgcttat cgcctcgcag gtatgggaca
     3181 tgaatttcga cagcgacact aacgccattg atgtggcggt gaagctgcta cgcggcaaaa
     3241 tcgacaacga ctttgagccg aagctgattc aaaccgtgcg cggcgtgggt tacatgcttg
     3301 aggtgccgga tggtcagtaa gccatttcag cgcccgtttt cgctggcaac ccgcctgacc
     3361 ttttttatca gccaggccac catcgcggcg tttttcgcct ttgcatggat cataatccac
     3421 tcagtaaaag tgcattttgc cgagcaggac attaatgatt taaaagagat tagcgccacc
     3481 cttgaacggg tactaaatca ccctgacgaa acgcaagccc gacgcttaat gacgctggaa
     3541 gatatcgtca gtggttattc caacgtgttg atttccctgg cagatagcca cggcaaaacg
     3601 gtgtatcact cccccggtgc gcctgatatc cgcgagtttg cacgtgatgc catacccgat
     3661 aaagacgcgc ggggtggcga ggtgtatctc ctttccggcc cgacgattat gatgccaggc
     3721 cacggtcacg ggcatatgga acacagcaac tggcggatga ttaacttgcc ggttggcccg
     3781 ttggtggacg gcaaaccgat ttatacgctc tacatcgcgc tgtcgatcga ttttcatctt
     3841 cattacataa atgatttgat gaataaactt attatgaccg catcggtaat cagcatcctg
     3901 atcgtcttta tcgtgctgtt ggcggtacat aaaggtcacg cgccgatccg cagcgtcagc
     3961 cgtcaaatcc agaatatcac ctcgaaagat ctcgacgttc gccttgaccc gcagaccgtg
     4021 ccgattgagc tggaacagct ggtactgtcg ttcaaccata tgatcgagcg tattgaggat
     4081 gtctttactc gccagtccaa tttctcagcg gatatcgccc acgaaattcg cacaccaatt
     4141 actaatctca ttacgcaaac ggaaatcgcc ctcagccagt cgcgcagcca gaaggagctg
     4201 gaagatgtgc tctactctaa tctcgaagag ctgacgcgaa tggcgaaaat ggtcagcgat
     4261 atgctgtttc tcgctcaggc cgataacaac cagctaatcc ccgaaaagaa aatgctcaac
     4321 ctggcggatg aagtcggcaa agtgttcgat tttttcgagg cgctagcgga agatcgcggc
     4381 gtggagttgc ggtttgttgg cgacaaacgt caggttgcgg gcgatccgtt gatgctgcgt
     4441 cgggcgctaa gcaacctgct ttctaatgcc cttcgttata cgccaaccag agagacaatt
     4501 gtagtacgct gtcagacggt cgatcaccag gtgcaagttt ccgtcgaaaa ccccggtacg
     4561 cccattgcgc ccgagcactt accgcgattg tttgaccgtt tctatcgcgt tgacccctcc
     4621 cgccagcgaa aaggtgaagg tagcggtatt gggctggcga tagtgaaatc gattgttgtc
     4681 gcgcataaag gcacggttgc ggtaacgtca gatgcgcggg ggacaaggtt tgttatcaca
     4741 ttacccgctt aattattcag caaacggcaa cttttataac cagtgtaaaa ataacgtgcc
     4801 gcaatagtca tacatattaa tggtaaaaag ctgtcacaat tcataaaaaa ccttaatata
     4861 cgccacccta aacataacca gcgttaatgt aaggtttttg tgtggactgg cttcttgatg
     4921 tttttgctac ctggctctac ggcttaaaag taatcgcgat aacgttagcg gtcatcatgt
     4981 tcatcagcgg gctggacgat ttttttattg atgtcgtcta ctgggtacgc cgcattaaac
     5041 gcaagttgag tgtttatcgc cgctacccgc gaatgagtta ccgcgaactg tataaaccag
     5101 atgaaaaacc gttagcgatt atggttccgg cgtggaatga aacgggcgtc atcggcaata
     5161 tggccgagct ggcggcgacc acgctcgact acgaaaacta tcatatcttt gttggcacct
     5221 accccaacga ccccgatact caacgtgatg ttgacgaagt gtgcgctcgc tttccgaacg
     5281 tgcataaggt agtctgcgcg cgtcctagtc ccaccagcaa agccgactgc ctgaacaacg
     5341 tgctggacgc catcacccag tttgaacgta gcgccaattt cgcttttgct ggttttattc
     5401 tgcatgacgc cgaagatgtg atttcaccga tggaattgcg tctgttcaac tatctggtcg
     5461 agcgtaaaga tctgattcag atcccagtgt atccgtttga acgcgaatgg acgcacttca
     5521 ccagcatgac ttacattgat gagttttcag aactgcatgg caaagatgtt ccggtgcgtg
     5581 aagccctcgc cggacaagtg cccagcgcag gcgtcggcac ctgtttcagc cgccgcgccg
     5641 tgaccgcact gttagctgac ggtaacggta ttgctttcga cgtgcagagt cttactgaag
     5701 attacgacat tggcttccgc ctgaaagaaa aaggtatgac ggaaattttt gtccgttttc
     5761 cggtggtgga cgaagccaaa gaacgcgagc agcgtaaatt tttacagcac gcacggacgt
     5821 caaacatgat ctgcgtgcgc gaatatttcc ccgatacctt ttcgactgcg gttcgacaaa
     5881 aatctcgctg gatcatcggc attgttttcc agggctttaa aacccataaa tggacctcca
     5941 gcctgacgct gaactacttt ctctggcgcg accgcaaagg ggcaatcagt aactttgtca
     6001 gcttcctcgc gatgctggtg atgatccagc ttttgctgtt gctggcgtat gaaagtttgt
     6061 ggcccgatgc ctggcatttc ctttctattt ttagcggcag cgcatggtta atgaccctgc
     6121 tgtggctaaa ctttggcttg atggttaacc gcatcgtgca gcgagtgatt ttcgtcactg
     6181 gctactacgg cctcacgcag ggcctgcttt ccgtcctgcg tcttttctgg ggcaacctga
     6241 ttaactttat ggctaactgg cgcgtgctga aacaggttct tcagcacggc gatccgcgtc
     6301 gcgtcgcgtc gcgtgggata aaacaacgca tgacttcccc agcgtcactg gcgatacccg
     6361 ctcgttgcgc ccgttaggtc aaattctgct ggaaaatcag gtcatcactg aagaacaact
     6421 cgatacagca ctgcgtaatc gcgtcgaagg tctacgcctg ggcggttcaa tgctgatgca
     6481 ggggctgatt agcgccgagc agctggcaca ggcgctggca gagcaaaacg gcgtggcgtg
     6541 ggaatccatc gatgcctggc agatcccgtc ctcgctgatt gccgaaatgc cagcctccgt
     6601 agcgctgcat tatgcagtac tgccgctgcg tctggaaaat gatgagttaa ttgtcggcag
     6661 tgaagatggt attgacccgg tttcgctggc ggccctgacg cgtaaagtcg gacgcaaagt
     6721 gcgttacgtc attgttctgc ggggacaaat tgtcacaggg ttacgccact ggtatgcacg
     6781 ccgacgcggt cacgatccgc gggcaatgtt gtacaatgcg gttcagcatc agtggctcac
     6841 ggaacagcag accggtgaaa tctggcggca atatgtgccg catcagttcc tgttcgccga
     6901 aatactgacc acgctcggtc atattaatcg ttcagcaatt aacgtgttgt tattgcgcca
     6961 tgaacgcagt tctctgccgc tcggcaagtt tttggtcacc gaaggcgtta tcagccagga
     7021 aacgttggat cgcgtcctga caattcaacg cgaattacaa gtttcgatgc aatcactatt
     7081 actcaaagca ggtttaaaca cagaacaggt tgcgcaactg gagtccgaaa atgaaggaga
     7141 ataaccttaa tcgcgtcatc ggatggtctg gtttactgct gacgtcttta ttgagtacca
     7201 gcgcactcgc agacaatatc ggcaccagcg cagaagagct ggggctgagc gattatcgcc
     7261 attttgttat ttatccccgt ctcgataagg cgctgaaggc acagaaaaat aacgacgaag
     7321 caaccgccat ccgcgaattt gaatatatac accaacaggt gccggataat attccgctga
     7381 ctttatacct tgcggaagcc tatcgtcatt ttggtcatga tgaccgggcg cggctgttgc
     7441 ttgaggatca actgaaacgt cacccaggag atgcccgact tgagcgcagt ctggcggcta
     7501 ttccagttga agtgaaaagc gttacgactg ttgaagaact gcttgcccag caaaaagcgg
     7561 gcgacgctgc gccgaccctg cgttgtcgca gtgaagtcgg gcagaatgcc ctgcggctgg
     7621 cacaattacc tgtcgccaga gcgcaactga acgatgcgac gtttgctgca tcgccggaag
     7681 gaaaaacgct gcgaaccgat ctgctgcaac gggcaatcta cctgaaacaa tgatcccagg
     7741 cagatacgct atacaatgaa gcacgccagc agaacacatt aagcgcggca gaacgccgtc
     7801 agtggtttga cgtgcttctt gccgggcagc tggacgatcg gatcctggca ctgcaatcac
     7861 aggggatctt caccgatcct cagtcatata ttacttacgc gaccgcgctg gcttatcgtg
     7921 gcgaaaaagc acgcctccag cattatctca ttgaaaataa gccgctgttt accacgggcg
     7981 cacaagagaa aagttggctc tatctgttat ctaaatacag cgccaacccc gttcaggcat
     8041 tggcgaatta tacggtgcag tttgccgata accgccagta tgttgttggc gcgacgctac
     8101 cggtgctgtt aaaagaaggt cagtacgacg cagcgcaaaa actgctcgcc accctccccg
     8161 ccaatgaaat gcttgaggag cgttatgctg tcagcgtggc gacccgtaac aaggctgaat
     8221 ctctgcgtct ggcacgattg ctgtatcagc aagaaccgac aaatcttccc cgcctggatc
     8281 aactgacctg gcaactgatg cagaacgagc agtcacgcga agctgccgat ttattgctgc
     8341 aacgctatcc tttccagggc gatgcgcgtg tcagccagac tttaatggcg cgactggcgt
     8401 ctctgctgga aagtcatcct tacctagcaa cgccggcgaa ggtggcgatt ttatcgaaac
     8461 ccttaccgct ggcggagcaa cgtcagtggc aaagtcagtt gccgggtatt gcagataatt
     8521 gcccggcaat agttcgcttg ctgggcgata tgtcgccttc ctacgatgct gccgcctgga
     8581 accgtctggc aaagtgttat cgggacacgc tacccggtgt ggcgttgtat gcatggcttc
     8641 aggccgaaca acgacaaccg agcgcctggc aacatcgtgc ggtagcctat caggcgtatc
     8701 aggttgagga ctacgccacc gcactggcgg cctggcagaa aatcagtctt cacgacatga
     8761 gcaatgatga tctgctcgcc gccgccaaca ccgctcaggc agcgggaaat ggcgcagctc
     8821 gcgatcgctg gcttcaacag gcagaacaac gtgagctggg aaacaatgct caatactggt
     8881 ggctgcatgc gcgacgttat atcccagctc agccagaact cgcactgaac gatctcacgc
     8941 gctcaatcaa tattgcccct tctgccaact cttacgttgc gcgggcgaca atttatcgcc
     9001 aacgtcataa tgttccggcc gcggtgagtg atttgcgcgc cgcgctggaa ctggaaccaa
     9061 ataatagcaa cacccaggca gcgcttggtt acgccttgtg ggatagcggt gatatcgcac
     9121 agtcgcggga aatgctcgaa caggcgcata aagggcttcc ggacgatccg gcactgatcc
     9181 gacaactggc ctacgtgaac cagcgtctgg atgacatgcc tgcgacgcag cactacgccc
     9241 ggctcgtgat tgatgacatt gataatcagg cataacccca ctgaccccag agcaaaatca
     9301 gcaacgcttc aatttccgcc gtttgcatga ggaggtcggt cgccgctgga cgttcagttt
     9361 cgattcttcc atcggcttgc gttccggcgc aatgagtacc gctaacaata atgtcggcgg
     9421 tgcagcgcct gggaaaagct atcgtagcta cggacaactg gaagccgagt accgcatcgg
     9481 acgcaatatg ctgctggaag gcgacctgct ctcagtttat agccgcgtct ttgccgatac
     9541 cggagaaaac ggggtgatga tgccggtgaa aaatccgatg tccggcaccg gtctgcgctg
     9601 gaagccgctg cgcgatcaga tctttttcct cgccgtcgaa cagcagttgc cgctgaacgg
     9661 gcaaaatggc gcatccgata ccatgctgcg cgccagcgcc tcattcttta atggcggcaa
     9721 atacagcgac gaatggcacc cgaacggttc aggctggttt gcccaaaacc tgtacctcga
     9781 tgcggcgcaa tatatccgcc aggatattca ggcgtggacg gcagattatc gcgtcagctg
     9841 gcatcagaag gtagctaacg gacagactat tgagccttac gctcacgttc aggacaacgg
     9901 ctatcgtgat aaaggcactc agggcgcgca gcttggcggt gtcggggtcc gctggaatat
     9961 ttggaccggc gagacgcact acgacgcctg gccgcacaaa gtcagtctcg gcgtcgagta
    10021 tcaacatacc tttaaggcga ttaatcaacg taacggagag cgcaacaacg cgtttctcac
    10081 cattggagtg cactggtaaa tgcgtaagtt cattttcgta ttgctgacac tgcttttggt
    10141 cagccctttt tcctttgcga tgaaaggtat tatctggcaa ccacaaaacc gagatagtca
    10201 ggttaccgat acccagtggc aggggctgat gagtcagtta cgttttcaag gcttcgatac
    10261 cctggttttg caatggaccc gttacggcga tgcatttacc cagccagaac agcgcacgtt
    10321 attgtttaag cgtgccgcag ctgcgcaaca gactggcctg aagctgattg tcgggctgaa
    10381 cgccgatccg gaatttttta tgcaccagaa acagtcgtct gcagcgctgg aaagctatct
    10441 taatcgcctg ctggctgccg atctccagca agccagatta tggagcgccg cgcctggcat
    10501 aacgccggat ggctggtaca tcagcgcgga aattgacgac ctgaactggc gcagcgaagc
    10561 cgcccgtcaa cctttgctaa catggttaaa caacgcgcag cggctgatta gcgatgtttc
    10621 agcaaaaccg gtttatatca gtagtttttt cgccggaaac atgtcgcccg atggctatcg
    10681 ccaactgctg gaacaagtta aagcaaccgg agttaatgtc tgggtacagg atggcagcgg
    10741 cgtggataaa cttaccgctg aacagcgtga acgttatcta caggccagcg ccgattgcca
    10801 aagctccgcc ccagcaagcg gcattgttta tgaactgttt gtcgccggca aaggcaaaac
    10861 ctttacagcg aaaccgaaac cggacgcaga aattgcctcg ctgttagcga aacgttcctc
    10921 ttgcggtaaa gacatgctct atttctctct gcgctatttg cccgtcgcgc acggcattct
    10981 cgagtattaa atcccctcca ggtaagtcgg gtacgacctg gcttacctct ttcgctctcg
    11041 tcagaattcc atcaagatta attgctaaaa agcatgctaa agaaagaaga tcgttatcgg
    11101 cgaacgggcc gatcactcat gcaggaagca tagatccttt gagcggagtt tctgatatct
    11161 gaggagtgcg aaatgcaatt gagcagcagt gaaccttgcg tggtgatcct gaccgaaaaa
    11221 gaggtagagg taagcgtcaa taaccatgct acgtttaccc ttccgaaaaa ctacctggcc
    11281 gccttcgcgt gcaacaataa cgtcattgaa ctctcaacgt taaatcacgt attaatcacc
    11341 cacatcaacc gtaacatcat caacgattat ctgttgtttt taaataagaa attaacctgt
    11401 gtaaagccct ggtcgcggct agcaaccccg gttatcgctt gtcatagccg tacaccggaa
    11461 gtgttccggc tagccaccaa ccacagcaag cagcaatcca gcaaaccctg cgaagcggag
    11521 ttgacgcgcg cattactttt taccgtattg tctaactttc ttgagcaatc gcggtttatt
    11581 gccctactga tgtatatctt acgcagcagc gtccgcgaca gcgtctgccg cattattcaa
    11641 agcgatattc agcattactg gaatctgcga attgtcgcca gttcgctatg tttaagcccc
    11701 agcctgctca aaaagaaatt aaaaaacgaa aataccagct atagccagat tgtcacagag
    11761 tgtcgtatgc gttacgccgt acagatgtta ttgatggata acaaaaatat cactcaggtg
    11821 gcgcaattat gtggctatag cagcacgtcg tactttatct ctgtttttaa ggcgttttac
    11881 agcctgacac cgttgaatta tctcgccaaa cagcgacaaa aagtgatgtg gtgaagggca
    11941 aagcggaaac ggataagacg ggcataaatg aagaagaaat ggcgcgccct gcaggattcg
    12001 aacctgcggc ccacgactta gaaggtcgtt gctctatcca actgagctaa gggcgcgttg
    12061 ataccgcaat gcggtgtaat cgcgtgaatt atacggtcaa cccttgctga gtcaatggct
    12121 tttgatctgg ttgctgaaca agtgaacgac cgcgtctgat tttctgattt atttcgctat
    12181 agcggcaaac aaacgcacac cgctgcgcgt ctgaatcaag aaaacccgta ttttcatgta
    12241 tcaaagtaca atttaccgcc ctaacggaaa attgtccgct cctatgagac tggtaactat
    12301 gaaaccaacg tcggtgatca ttatggatac tcatcctatc atcagaatgt ctattgaagt
    12361 tctgttgcaa aaaaacagtg aattgcagat tgtcctgaaa acggatgatt accgcataac
    12421 catcgattat ctccgaaccc gtcctgttga tttaatcatt atggatatag acttgcccgg
    12481 aacagacggt tttaccttcc t
//
LOCUS       DADLTY010000124        12427 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.124, whole genome shotgun sequence.
ACCESSION   DADLTY010000124 DADLTY010000000
VERSION     DADLTY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.124"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JN334_002942"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JN334_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAY7439013.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JN334_002943"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JN334_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HAY7439014.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JN334_002944"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JN334_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HAY7439015.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JN334_002945"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JN334_002945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HAY7439016.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="JN334_002946"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="JN334_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HAY7439017.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="JN334_002947"
     CDS             complement(8217..9110)
                     /locus_tag="JN334_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HAY7439018.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JN334_002948"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JN334_002948"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAY7439019.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /locus_tag="JN334_002949"
     CDS             complement(10007..10189)
                     /locus_tag="JN334_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HAY7439020.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="JN334_002950"
     CDS             complement(10241..11473)
                     /locus_tag="JN334_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAY7439021.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..>12427)
                     /gene="lpxK"
                     /locus_tag="JN334_002951"
     CDS             complement(11510..>12427)
                     /gene="lpxK"
                     /locus_tag="JN334_002951"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAY7439022.1"
                     /translation="GLVSGAIRLCYKLKLKRAWRAPVPVVVVGNLTAGGNGKTPVVVW
                     LVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTTTAQAGDEPVLIYQRTDAPVAVSP
                     VRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVEIVVIDGVRRFGNGWWLPAGPMRE
                     RAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNLRTGTRCDVAQLEHVVAMAGIGHP
                     PRFFATLKMCGVQPEKCVPLADHQSLNHADVSALVSAGQTLVMTEKDAVKCRAFAEEN
                     WWYLPVDAQLSGDEPAKLLTQLTSLASGN"
BASE COUNT         2869 a         3538 c         3083 g         2937 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcaccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggcc
//
LOCUS       DADLTY010000125        12219 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.125, whole genome shotgun sequence.
ACCESSION   DADLTY010000125 DADLTY010000000
VERSION     DADLTY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.125"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..492)
                     /locus_tag="JN334_002952"
     CDS             complement(<1..492)
                     /locus_tag="JN334_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439023.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(523..1215)
                     /gene="fimC"
                     /locus_tag="JN334_002953"
     CDS             complement(523..1215)
                     /gene="fimC"
                     /locus_tag="JN334_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY7439024.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="JN334_002954"
     CDS             complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="JN334_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein subunit FimA"
                     /protein_id="HAY7439025.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2458..3324
                     /gene="folD"
                     /locus_tag="JN334_002955"
     CDS             2458..3324
                     /gene="folD"
                     /locus_tag="JN334_002955"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAY7439026.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3326..3538
                     /gene="ybcJ"
                     /locus_tag="JN334_002956"
     CDS             3326..3538
                     /gene="ybcJ"
                     /locus_tag="JN334_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HAY7439027.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3646..4155
                     /locus_tag="JN334_002957"
     CDS             3646..4155
                     /locus_tag="JN334_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7439028.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="JN334_002958"
     CDS             complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="JN334_002958"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAY7439029.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5750..6244
                     /gene="ppiB"
                     /locus_tag="JN334_002959"
     CDS             5750..6244
                     /gene="ppiB"
                     /locus_tag="JN334_002959"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HAY7439030.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6247..6969
                     /gene="lpxH"
                     /locus_tag="JN334_002960"
     CDS             6247..6969
                     /gene="lpxH"
                     /locus_tag="JN334_002960"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAY7439031.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7087..7596
                     /gene="purE"
                     /locus_tag="JN334_002961"
     CDS             7087..7596
                     /gene="purE"
                     /locus_tag="JN334_002961"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAY7439032.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7593..8660
                     /gene="purK"
                     /locus_tag="JN334_002962"
     CDS             7593..8660
                     /gene="purK"
                     /locus_tag="JN334_002962"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAY7439033.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8797..9690)
                     /locus_tag="JN334_002963"
     CDS             complement(8797..9690)
                     /locus_tag="JN334_002963"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAY7439034.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9687..10334)
                     /locus_tag="JN334_002964"
                     /pseudo
     CDS             complement(9687..10334)
                     /locus_tag="JN334_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10337..11569
                     /gene="allC"
                     /locus_tag="JN334_002965"
     CDS             10337..11569
                     /gene="allC"
                     /locus_tag="JN334_002965"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HAY7439035.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11580..>12219
                     /gene="allE"
                     /locus_tag="JN334_002966"
     CDS             11580..>12219
                     /gene="allE"
                     /locus_tag="JN334_002966"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HAY7439036.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2966 a         3157 c         3117 g         2979 t
ORIGIN      
        1 agcgctactc aacaacgcta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc
       61 aaagttaatt gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg
      121 ctgggctaac tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt
      181 aaaacagggc attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac
      241 gatatcctgt gaaccaatga actcatcatt acgccagaga tccacccgat acacgcccgc
      301 aggttgatga ttacctctct caaagcgcga taaatcagca acggatgtgc cattctctaa
      361 taaaaatgcc gggttgaaat aactttccgc atagcttaaa aaggagggta aaatggcaag
      421 agatgaataa caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt
      481 gggtattttc ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc
      541 tctgaccggg gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac
      601 tcctgagggt aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt
      661 ttggctgccc acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt
      721 gatatagtta ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg
      781 taattgaatt ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt
      841 cctgccgttc aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag
      901 agactcacga tctgccgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct
      961 tttgggttca ctaacaaata gtggcggtgt aatgatgaat gacttttctt ttacaccgct
     1021 gctgttttca atccacgaat tgaccagaaa gagctcattg gtatggctat ttttaatcca
     1081 taccgcagtc tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc
     1141 tccggcagca tgggcgctgg ccgaaatgat taaataaaat atcatgagca tcaataactt
     1201 tattttagtc atcatagtta atacgccttg tgtattcctg acggcaaaaa taaggagtat
     1261 aaagaaatga ttgctttggc ttcttacaac acatcattcc cttttcattt aataaagcgg
     1321 catgatacaa agtgaacgtg gtttcgattt acattgccac cataccgctt cagacaaccg
     1381 gtacattact ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc
     1441 gtatctcata ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt
     1501 gcctttataa cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt tctggttggt
     1561 tgagaagcta ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc
     1621 gacacccgtt gctgttgttg tatttgtact ggatacaatg gccaataaat tatcattgat
     1681 tgcatcagcc tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc
     1741 gttcaactga atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc
     1801 ggtacgatat tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc
     1861 agcattcacc agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact
     1921 acccgtagcg gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata
     1981 tattccttca atctcattta tcgactccac atccgtatat aaccgattac tttatttaag
     2041 acactgatag tagtaaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca
     2101 ctctattcta aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcac
     2161 cacaaatata gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg
     2221 tatttcttaa attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg
     2281 ccatcatgta cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac
     2341 cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct
     2401 tctgacaaaa tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg
     2461 gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa
     2521 aaagttcagg ctcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg
     2581 ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc
     2641 gggttcgtct cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag
     2701 cttatcgatg cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta
     2761 ccggcgggta ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac
     2821 ggtttccatc cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc
     2881 accccgcgcg gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac
     2941 gccgtggtga ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc
     3001 gcaggttgca ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa
     3061 aatgccgatc tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc
     3121 aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg
     3181 ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc
     3241 ggcgttggcc cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa
     3301 tatcatgatc cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt
     3361 tgagctgtgc gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat
     3421 cgcgattgcc gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa
     3481 aatcgtcgcc ggtcagacag tgagttttgc aggacacagc gtacaggttg ttgcctgatc
     3541 cctttcccgg cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc
     3601 gttcatccat gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt
     3661 acgcacgccg ccgttcccct ttgcattggt ttagggttgg gttcgaaagg tctgttattt
     3721 gccggaatca tcctggcgat gctgcccgat gccgacgtat tgtcgtttaa atttggcgtt
     3781 gcttacggca atgtttttgg tcatcgcggt tttacccatt cgctggtgtt tgcgtttgtt
     3841 gtcccgctct tatgtgtgct gattggacgt cgatggttca gggctgggct gattcgctgc
     3901 tggctgtttt taaccgtctc gttgctatcg cacagcttac tggattcggt aaccactggc
     3961 ggtaagggcg ttggctggct gtggccgtgg tcagatgaac gctttttcgc tccctggcag
     4021 gtgattaaag tcgcgccgtt tgcattgtct cgttacacca cagcgtacgg gcatcaggtg
     4081 attatttccg aattgatgtg ggtgtggttg ccggggatgc tactgatggg aatgttgtgg
     4141 tggcgcagac gataaccgga tgcgaaaact cgcatccggc aattgcgcaa ttacttacga
     4201 cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt aagacggtca
     4261 cgtgccgcat ctgccgccgc ccagtctttc gctttacggg catccagacg ctgttgaatt
     4321 aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct
     4381 tccggttctt gctccagcag gcccaataca gcggaaagtt tacgcaggtg agatgccatt
     4441 gcattcgccg ctgccatatc ttctgctttc agacggttca cttcacgcgc catatcaaac
     4501 agcacggaat aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct
     4561 tcaaacgctt cgccaccggc aggcgcaacg gttttatctg tgccgcgcag cgcagtgtag
     4621 aggcgctcca gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt cagctggctg
     4681 cgatagtggc ccgacatcag gaagtaacgc acggtttccg cgtcgtagta tttcagcaca
     4741 tcgcgcacgg taaagaagtt acccagcgat ttggacatct tctcgcggtc aaccatcacc
     4801 attccggagt gcatccagta gttcacatac tgaccatcat gggcacaggt ggactgcgcg
     4861 atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat atcaaagtgg
     4921 ttacccagct gcttgcagtt catggccgaa cattcaatgt gccagccagg acgacccgcg
     4981 ccccacggag acggccagct cggttcgccc tctttcgaca tcttccacag aacgaagtcc
     5041 atccggttgc gtttgtcatc aaccacgtca acgcgcgcgc cagcctgcag ctggtcgaga
     5101 tcctgacgcg acagcacgcc ataagttgga tcggtcggga cgtcgaacat cacgtcgctg
     5161 ttgtccgcca cataagcgtg acctttggcg atcagttgtt cagtgagttc aataatttct
     5221 gcgatatggt gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa agcatcaaaa
     5281 tctttgtgca tttcggcgat catgcgatcc accagcgcca caaagctttc gccattttca
     5341 ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata cttcagttta
     5401 tagccgagga aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga
     5461 ccgatatgac agagatcgta aacggtgatt ccacacacgt acatgccgac ttccccggcg
     5521 tgaataggct taaattcctc tttttggcgt gtcagagtat tgaagatttt tagcatcgaa
     5581 gattccgttt agacatgtgt gggtaattga gttgcgtata atacccatat ttcccgcgcg
     5641 aatcagcata cattgcgtga tgatccgatc gtgcggttat gctataacac caccctatat
     5701 atgacccgaa ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa tggttacttt
     5761 ccacaccaat cacggcgata ttgtcatcaa aacttttgac gataaagcac ctgaaacagt
     5821 taaaaacttc ctggactact gccgcgaagg tttttacaac aacaccattt tccaccgtgt
     5881 tatcaacggc tttatgattc agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac
     5941 caaagaaccg atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg gtacgctggc
     6001 aatggcacgt actcaggctc cgcactctgc aactgcacag ttcttcatca acgtggttga
     6061 taacgacttc ctgaacttct ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc
     6121 tgaagtggtt gacggcatgg acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtaa
     6181 cggtatgcac caggacgtgc caaaagaaga cgttatcatt gaaagcgtga ccgttagcga
     6241 gtaatcgtgg cgacactctt tattgcagat cttcatctct gcgtggaaga accggcgatc
     6301 accgccggtt ttctgcgttt tttagcgggg gaagcccgca aggccgacgc gctgtatatt
     6361 cttggcgatc tgtttgaagc atggattggc gacgacgatc ccaacccact ccatcgccag
     6421 atggcggcag cgatcaaagc ggtgtccgat tccggcgttc cctgttattt cattcatggc
     6481 aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac attattgccg
     6541 gaagaaaagg tgctcgaact ttatggtcgc cgggtgttga ttatgcatgg cgacacgctg
     6601 tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa
     6661 atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgcgcg
     6721 aacagcaaag aagccaacag cagtaaatcg ctggcgatca tggacgttaa ccaaaacgct
     6781 gtggtcagtg cgatggaaaa acatcaggtg caatggctaa tccacgggca tacccatcgc
     6841 ccggcggtgc atgaacttat cgccaaccag caacctgctt ttcgcgtggt actgggtgcc
     6901 tggcatacgg aaggttcgat ggtgaaagtc acggctgatg acgttgagct gattcatttt
     6961 ccgttttaaa aaacccacaa ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc
     7021 tctttccgtg ctattctctg tgccctctaa agccgagagt tgtgcaccac aggagtttta
     7081 agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc
     7141 gactgggcta ccatgcagtt cgccgtcgaa atcttcgaaa tcctgaatgt cccgcaccac
     7201 gttgaagtgg tttctgctca ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc
     7261 gaagagaacg gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca cctgccaggc
     7321 atgattgccg ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg
     7381 agcggtgtcg atagcctcta ctccatcgta caaatgccgc gcggcattcc ggtgggtacg
     7441 ctggcgattg gtaaagctgg cgcggcaaac gcggcattac tggcagcaca aattctcgcg
     7501 actcacgata aagagctaca ccagcgtctg aatggctggc gcaaagccca gaccgacgaa
     7561 gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc ctcggtaacg
     7621 ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct gtctggccgg
     7681 tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt accgctgaga
     7741 tcgaacgctg gccggaaacc gcattaaccc gcgagctggc gcgtcatccg gcctttgtga
     7801 accgcgatgt gttcccgatt attgctgacc gtctgactca gaaacagctt ttcgataagc
     7861 tccacctgcc gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt
     7921 ttgatcgttt aggtgaactg gcgattgtta agcgtcgcac tggtggctat gacggtcgcg
     7981 gtcaatggag tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt tacggtgaat
     8041 gtattgtcga gcagggcatt aacttctctg gtgaagtatc gctggttggc gcgcgcggct
     8101 ttgatggcag caccgtgttt tatccgctga cgcataacct gcatcaggac ggtattttgc
     8161 gcaccagcgt cgcttttccg caggccaacg cacagcagca ggcgcaagcc gaagagatgc
     8221 tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat ggcgatggag tgttttgtca
     8281 ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt gcataacagc ggtcactgga
     8341 cacaaaacgg tgccagcatc agccagtttg agctgcatct gcgggcgatt accgatctgc
     8401 cgttaccgca accagtagtg aataatccgt cggtgatgat caatctgatt ggtagcgatg
     8461 tgaattatga ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc
     8521 gtccggggcg taaagtgggg catctgaatt tgaccgacag cgacacatcg cgtctgactg
     8581 cgacgctgga agccttaatc ccgctgctgc cgccagagta tgccagcggc gtgatttggg
     8641 cgcagagtaa gttcagttaa gcaggtgctc atttgattgc cggatgcggc gtaaacgcct
     8701 tatccggctt accgctccgg tacccattgt aggcctgata agacgcgtca agcgtcgcat
     8761 caggcatggt gcaccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc
     8821 ttcgcccgcc agcgtctctt caattcgcga taacgcccca atccacgcgg gtttactacg
     8881 gcttctgaca taaccactca ccgccgttac cttcggcccc atcgaaccat cggctttggc
     8941 aaatggcgct aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca
     9001 gttttcatat accgcatcag catcggtgag gatcaccagt ccatcggcat taatctgctc
     9061 ggcgagcaac gcagcggcga gatctttatc aatcaccgct tcactccctg ccccatcttc
     9121 cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa
     9181 ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa
     9241 atatttacca tcacgtttca tctgccagcc gtaagccgct tccagtgcct cttgttcttc
     9301 tggctgataa accggaccaa taaatttttc tggctgcaaa aacgccggat catcaggcga
     9361 aacctcaatg cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct
     9421 ctgcgccagc atatagccaa tcatcccctg gctttccgcc accagtacat ccagcggata
     9481 cggttctacc tctttccacg ccagattctg taacgccagc agccccacct gcggcccgtt
     9541 gccgtgaaca atcgccagcc gataagaacg ggccaggcgt gccagcgcgg gtacagcact
     9601 ggcgatattg cgatattgat tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt
     9661 gcccccaaga gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc
     9721 cagaacccca gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc
     9781 gcaacagcgg tacgtttcgg gcaactcaga gcatggacaa agtgcaggag tggcgaggcg
     9841 aaatatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga
     9901 agattgtcgg aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc gccataatac
     9961 caggccgcca gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa tccggggcct
    10021 ttacccagcc agtgccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga
    10081 cgaaactggc gtaattcagc gggcagcgga tcgcttgccg ccatcgccag tggcccgaaa
    10141 agtccggttt cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg atgcgaaggc
    10201 ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga
    10261 atcccttgag ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg
    10321 aattgcgtgc ggcgaaatta cacattcccg tcaagctata gaagaaacgc tgccctggct
    10381 ttcctctttt ggcgctgacc caacgggtgg gatgacccgt ttactttatt cgccggaatg
    10441 gctggaaacc cagcagcaat ttaaaaaaag aatggcagca agtgggctgg aaacacgttt
    10501 cgatgaagtg gggaatttat acggtcgcct gagtggcacc gaatatccac aggaagtggt
    10561 tctgagcggt tcgcatatcg ataccgtggt taacggcggt aacctcgacg ggcaattcgg
    10621 cgcgctggcg gcgtggctgg caattgactg gttgaaaacg caatacggcg caccgttacg
    10681 tacggtcgaa gtggtggcga tggcagaaga agaaggcagc cgcttcccgt atgtcttctg
    10741 gggcagtaaa aatatctttg ggctggcgaa tcctgacgac gtgcggaata tctgtgatgc
    10801 caaaggaaat agttttgccg atgcgatgaa ggcttgcgga tttactctgc cgaacgcccc
    10861 actaactccg cgtcaggcta taaaagcctt tgttgaactg catattgaac agggctgtgt
    10921 gctggaaagt aatgggcaat caattggcgt ggtgaatgca attgtcgggc agcgtcgcta
    10981 tacggtgacg ctgaacggcg aatcaaacca tgcaggcacc acgccgatgg gttatcgtcg
    11041 tgatacggtt tacgctttca gtcgcatttg ccatcagtcg atcgaaaaag cgaaaaagat
    11101 gggcgatccg ctggttctga cctttggcaa agtagagccg cgcccgaata cggtaaatgt
    11161 ggtgccgggt aaaaccacgt tcaccattga ttgtcgtcat accgacgctg ccgtgctgcg
    11221 cgatttcacc caacagttag aaaacgacat gcgggcgatt tgcgatgaaa tggacattgg
    11281 tattgatatc gatttatgga tggacgaaga acccgtgccg atgaataagg agctggtcgc
    11341 caccctgaca gaattgtgtg aaagagaaaa actgaattac cgggtgatgc acagtggtgc
    11401 cgggcacgac gcgcaaattt tcgcgcctcg cgtaccaacc tgcatgattt ttatccccag
    11461 catcaacggg atcagccata acccggcgga acgcaccaat attactgacc ttgccgaagg
    11521 ggtcaaaacg ttggcactca tgctttatca acttgcctgg caggaataag gagtcataaa
    11581 tgggatattt aaataacgtc accggttacc gcgaagattt actggctaac cgtgcgattg
    11641 ttaaacacgg taatttcgca ctgttaaccc cggatggtct gataaaaaat attattccgg
    11701 actttgaaaa ttgtgacgcg acaatcctct ccacgccaaa gctgggtgcc tcttttgttg
    11761 attatctggt cacactgcat caaaacggtg gcaaccaaca gggcttcggt ggcgaaggca
    11821 ttgaaacgtt cctgtatgtg atctctggaa atatcactgc caaagccgaa ggcaaaacat
    11881 ttgccttaag cgaaggtggc tatctttatt gcccaccagg ctccttaatg acgtttgtta
    11941 acgcccaggc cgaagacagc caaatctttt tatataagcg ccgctatgtt ccggtagaag
    12001 gccatgcacc gtggctgttt tctggcaatg ccagcgaact ggaacgcatt cactatgaag
    12061 gtatggatga tgtgatcctg ctggattttc tgcccaaaga gttaggtttt gatatgaaca
    12121 tgcatatcct ctcttttgca ccaggtgcca gccacggtta tatcgaaaca cacgttcagg
    12181 aacatggtgc ctatattctt tccggtcagg gggtttata
//
LOCUS       DADLTY010000126        12151 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.126, whole genome shotgun sequence.
ACCESSION   DADLTY010000126 DADLTY010000000
VERSION     DADLTY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.126"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            126..995
                     /locus_tag="JN334_002967"
     CDS             126..995
                     /locus_tag="JN334_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439037.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="JN334_002968"
     CDS             complement(1198..1551)
                     /locus_tag="JN334_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAY7439038.1"
                     /translation="MHVKYLVGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="JN334_002969"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="JN334_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAY7439039.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="JN334_002970"
     CDS             complement(3379..3672)
                     /locus_tag="JN334_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAY7439040.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="JN334_002971"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="JN334_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAY7439041.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="JN334_002972"
     CDS             complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="JN334_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAY7439042.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="JN334_002973"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="JN334_002973"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAY7439043.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /locus_tag="JN334_002974"
     CDS             7587..8888
                     /locus_tag="JN334_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY7439044.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="JN334_002975"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="JN334_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAY7439045.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /locus_tag="JN334_002976"
     CDS             9318..11015
                     /locus_tag="JN334_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAY7439046.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="JN334_002977"
     CDS             11052..11627
                     /locus_tag="JN334_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7439047.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="JN334_002978"
     tRNA            11734..11809
                     /locus_tag="JN334_002978"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2931 a         3139 c         2979 g         3102 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tactaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agatatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctag cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggccgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 agatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa c
//
LOCUS       DADLTY010000127        12135 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.127, whole genome shotgun sequence.
ACCESSION   DADLTY010000127 DADLTY010000000
VERSION     DADLTY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12135
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.127"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(139..3654)
                     /locus_tag="JN334_002979"
     CDS             complement(139..3654)
                     /locus_tag="JN334_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HAY7439048.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="JN334_002980"
     CDS             complement(4107..5369)
                     /locus_tag="JN334_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY7439049.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="JN334_002981"
     tRNA            complement(5567..5642)
                     /locus_tag="JN334_002981"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="JN334_002982"
     CDS             complement(5748..6455)
                     /locus_tag="JN334_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAY7439050.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6853..8988
                     /locus_tag="JN334_002983"
     CDS             6853..8988
                     /locus_tag="JN334_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY7439051.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /locus_tag="JN334_002984"
     CDS             complement(9037..10293)
                     /locus_tag="JN334_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HAY7439052.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="JN334_002985"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="JN334_002985"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAY7439053.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="JN334_002986"
     CDS             complement(11639..11914)
                     /locus_tag="JN334_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAY7439054.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..>12135)
                     /locus_tag="JN334_002987"
     CDS             complement(11942..>12135)
                     /locus_tag="JN334_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY7439055.1"
                     /translation="TAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSV
                     GLAAPVERLLQQLRTGAPV"
BASE COUNT         3018 a         2995 c         2931 g         3191 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtca
//
LOCUS       DADLTY010000128        12111 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.128, whole genome shotgun sequence.
ACCESSION   DADLTY010000128 DADLTY010000000
VERSION     DADLTY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.128"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..369
                     /locus_tag="JN334_002988"
     CDS             <1..369
                     /locus_tag="JN334_002988"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439056.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(457..1101)
                     /gene="cheZ"
                     /locus_tag="JN334_002989"
     CDS             complement(457..1101)
                     /gene="cheZ"
                     /locus_tag="JN334_002989"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HAY7439057.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1112..1501)
                     /gene="cheY"
                     /locus_tag="JN334_002990"
     CDS             complement(1112..1501)
                     /gene="cheY"
                     /locus_tag="JN334_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheY"
                     /protein_id="HAY7439058.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1516..2565)
                     /locus_tag="JN334_002991"
     CDS             complement(1516..2565)
                     /locus_tag="JN334_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HAY7439059.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2568..3428)
                     /gene="cheR"
                     /locus_tag="JN334_002992"
     CDS             complement(2568..3428)
                     /gene="cheR"
                     /locus_tag="JN334_002992"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HAY7439060.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3447..5048)
                     /gene="tap"
                     /locus_tag="JN334_002993"
     CDS             complement(3447..5048)
                     /gene="tap"
                     /locus_tag="JN334_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HAY7439061.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5094..6755)
                     /gene="tar"
                     /locus_tag="JN334_002994"
     CDS             complement(5094..6755)
                     /gene="tar"
                     /locus_tag="JN334_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HAY7439062.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6900..7403)
                     /gene="cheW"
                     /locus_tag="JN334_002995"
     CDS             complement(6900..7403)
                     /gene="cheW"
                     /locus_tag="JN334_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HAY7439063.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7424..9388)
                     /gene="cheA"
                     /locus_tag="JN334_002996"
     CDS             complement(7424..9388)
                     /gene="cheA"
                     /locus_tag="JN334_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HAY7439064.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9393..10319)
                     /gene="motB"
                     /locus_tag="JN334_002997"
     CDS             complement(9393..10319)
                     /gene="motB"
                     /locus_tag="JN334_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HAY7439065.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10316..11203)
                     /gene="motA"
                     /locus_tag="JN334_002998"
     CDS             complement(10316..11203)
                     /gene="motA"
                     /locus_tag="JN334_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HAY7439066.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11330..11908)
                     /gene="flhC"
                     /locus_tag="JN334_002999"
     CDS             complement(11330..11908)
                     /gene="flhC"
                     /locus_tag="JN334_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HAY7439067.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11911..>12111)
                     /locus_tag="JN334_003000"
     CDS             complement(11911..>12111)
                     /locus_tag="JN334_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HAY7439068.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2782 a         3387 c         2934 g         3008 t
ORIGIN      
        1 actacgctaa tgagttggga tgccgtgctc tatgatctac gtagtgaaaa aattatcagt
       61 aaagataatt acttgaccga cttaaaccgc agacatcttg aacttgttct tcatactaac
      121 cccaatccaa gcggttcggt aacgaagatt ttacgcacta tttttactaa gcaggtaaag
      181 agtttagggc cggaactctg tcatttcttg caccatgaac tgacgaaaaa acgttatgaa
      241 gtatggcatc actacgatgt actgcactac cagtcatcgt cattttctcg ttcacaattt
      301 aatgcattga taaatttact ggcgcaacaa tcacgcttcg ctgatctaaa attatcaaac
      361 tacaaataac catcctccac gtactccctg aacgataccc gccaccctgc tatcggcttg
      421 gcatcatcgc gcaggccgaa gttcaggcaa tacaaatcaa aatccaagac tatccaacaa
      481 atcgtccacc tgatcctgac tggctaccac gccggctttg ctggtatcga cctgaggtcc
      541 attaagcaaa ctctggtttt cacgttttgg acgcgactcc tgttccggga tgttttccaa
      601 cagcaccatc agcaactggc gttcgatctc ctgaatgaca tccatcatcc gcttaataac
      661 ctgcccggtg agatcctgaa aatcctgcgc catcatgatt tccagcagtt gcgcgttagt
      721 aaagctggta tgcgcgggta catctgccag aaattgtcgt gtatctgtta ccagttcacg
      781 ggcgtcggca aggtcaatcg gatcggcaaa ccagtcatcc caacgttggg ttaacgcttt
      841 tgctgatttc tccatttgat cctgatgcgg ttgtgacgcc tcaacactgt tcagcgcccg
      901 ctccgcagcc tgggcggtca tctgcacaac atagtacaaa cgatcgcgcg catcggggat
      961 ggcttccgcc gcttcggcaa tggcctgaac cagccccagt tcccgcaaac tgtcgcgcag
     1021 catatgcgtc aggctgccga tgcgcgcaat gatatcgcca gctgaatgct cgtcagcagg
     1081 tttgattgat ggttgcatca tagtcgcatc ctcacatgcc cagtttctca aagattttgt
     1141 tgagtttttc ctccagcgtc gcggcggtaa atggcttcac cacatagcca ctggcccccg
     1201 cttgcgccgc agcaatgatg ttctctttct tcgcttctgc agtcaccatt aacactggca
     1261 atgccgacat cgcgccatcc gcacgaattg ttttcagcaa ttccaggcca tccatattgg
     1321 gcatgttcca gtcggagata acaaatccat aaccgcctgc ctgcaactta ttgagagcgt
     1381 cgacaccatc ttccgcttcc tcaacattat tgaatcccag ctctttcagc aggttacgca
     1441 ctatgcgtcg catggtggaa aagtcatcca caaccaaaaa tttaagttct ttatccgcca
     1501 tttcacactc ctgatttaaa tacgtatcgc ctgtccggca ctaattgttg ccaacatttg
     1561 ctggcttacc tggctaagat cgaccacttc gcagacacca cccatattga tggcctcgcg
     1621 cggcatgccg aacaccacgc aacttgcttc gttttgcgca agggtccatg cccccgcctg
     1681 acgcatcgcc aacattcccg ccgcgccgtc gttgcccata ccggtcagga tcaccccaac
     1741 cgcattacgc cccgcctgtt tggcgacaga atggaacaac acatctaccg aaggccgatg
     1801 acggttaacc gccgggccgt cgtgaatttt gatttgataa tttgcgccac tacgcgccag
     1861 ttccatatgc cgatcgcccg gcgcaatata ggcatgcccc ggcaagacac gttctccgtc
     1921 ttcggcttct ttaaccccga tctggcaaag cttattaagt ctgtcggcaa aagagcgggt
     1981 gaaaccgggc ggcatatgtt gggtaattaa caatgccggg ctggaaagcg gcaacggttg
     2041 cagtacgtga cgaattgcct cagttccacc cgttgaagca ccaatcgcaa tcagtttttc
     2101 agaactcaac aacggccctg ccttcagcgt tgtcggtgct gacaatggct tatgtgctgc
     2161 aaggctcgcc tttgctgccg tacgcacctt ttcagcaatc atttcgctat acgccagcat
     2221 tccttcgcga atacccagtt gcggtttggt gacaaaatct attgccccca gctccagcgc
     2281 gcgcagcgtg acttctgatc ctttgccggt caaggaagaa accatcacaa cgggcattgg
     2341 acgcaaacgc attaattttt cgaggaaatc cagtccgtcc atccgcggca tttcaacatc
     2401 cagcgtcagc acatcgggat tgaatttctt aatcaagtca cgcgcaacca gcggatcagg
     2461 cgcggtcgcc accatttcca tgtcgctatg gctgttgatg atttctgtca tgatctggcg
     2521 catcagtgcc gaatcatcga cagataacac cctgattttg ctcatcgtta atccttactt
     2581 agcgcataca ccgtctgacc acgcagcgtg aagcggcgct cgaggtggct aaagttttca
     2641 gagtgacccg caaacagcaa tccgtcgggt ttaaggagcg gaacaaagcg gcgcaaaatc
     2701 tcctgctggg tagtttgatc gaagtagatc atgacgttac gacagaagat cgcatcaaac
     2761 ggccccggca cggtgtactg tttcgccagt aaattcagcg gggcaaaatc aacatagttc
     2821 gccagctcct gacgcacgcg taccagccct tcatgcggtc ccgtccctcg catgaaatac
     2881 cgttgcagtt gctgcggcgt caggtttttc aactcttcat ggcgatagat accgcttctg
     2941 gctttttcca gcacttcggt gtcgatatca ctggcaaaca ctttccagcg tccgggcacg
     3001 gtgcccaatg tgtcagccag agtcatcgca atgctgtacg gctcttcgcc ggtcgaagcc
     3061 gccgcgctcc atacgcgatc ctcgccagaa cggcgacgtg cgtgatccgc gagcagaggg
     3121 aaatgatgag cctcacggaa aaatgccgtc agattcgtgg tcagcgaatt gataaacgcc
     3181 tgccactcac cgctgtgctg attagattcc agcaagttca gataatgacc gaaatccgtc
     3241 agtcccagag aacgcaaacg acgaaccagt cggttgtaaa ccatgtcgcg tttatggtca
     3301 gccagaacga tcccggctcg ttgatagatc aattgactta tccgccgaaa atgcgcgtcg
     3361 gaaagcgcca ggcgctcggt catctgtaac aataaagacg tttgcccaca gggcagagat
     3421 gaagtcatag cgccttctca atcacttcag gataccactg gcgcaatttg taactgcgcc
     3481 gactcatgtc gtgctacttc atgttcttca agggtaaata ccgccacacg cgacgaaaga
     3541 tggtcggcct gattcgccag ttgttccgtt gccaccgccg cttcttctac cagcgaggag
     3601 ttctgctgag tcacctgatc catctggctg acagcctgtg caacctgctc aatcccccgc
     3661 cgttgttctt ccgacgccga agcaatttct cccataatgt cgttcacgcg ggtcaccgaa
     3721 ctgacaatat cggtcatggt cgcggcggcg ttattcacca gtttcgaacc ctgctggaca
     3781 cgattgactg actcttcgat cagccctttg atctcttttg ctgcctgcgc gctacggctg
     3841 gcaagattgc gcacttcacc tgccactacc gcaaaaccac gcccctgctc tccggcgcga
     3901 gccgcttcca ctgccgcatt cagggccaga atattggtct ggaaagcaat tccgtcgata
     3961 acgctgataa tgtcgccaat tttttgcgag ctggtggcga tctcctgcat ggtgtgagtc
     4021 atggtactga cctgaacacc gcccgcctgc gccgttgtcg cggcattttt tgccagttcc
     4081 gacgcctgtc gtgcgttatc ggcgttttgc cctaccgtgg cggttaattg ctccatactg
     4141 gcggccgttt gtgccagcga tgccgcctgc tgttcggtac gacttgagag atcgttattg
     4201 cctgcgacaa tctccgcgat cccaatgtgc atctcatggc ttcccttacg cacatcactc
     4261 accgtcccac gcaaagcctg ctgcatggtc ttcaggctgg caaaaatggc ggtgatctca
     4321 ttacgaccat ataccgcaat cggacgcgcc agattacccg cagcaatgct gtcaaaatgg
     4381 ctaccgataa tggccagtgg ttgaacaatc atcttgcgcg tccaccacag cgcactgctg
     4441 atgtagatcg ccgcaacaat aatcatgctg ataaacacca gcgccgaaat ctgatagtta
     4501 cgctggcttt gcgcactggc ggcttccagc acatggttga tctccagctg ccaggcctca
     4561 aagttaacgt caaacgcatt ctgtgatccc tgcaccggcg cagtgaggaa atccgaaagt
     4621 tgattacttt caagccaggt cgcctgatgt tcgagatcgt tgtgccagcg ggcaaaactt
     4681 ttctccgtct ctttttgcaa tgccctgacg tgctcgttgc ccgcagtcat cgccataaaa
     4741 cttttaaaca gcgtggtgga ttgcgtcaga ctggcgcgcg ccgtcgtcat caacgtttta
     4801 atgtcatccg ccggatagct aagcgctgtc agagtgcctg ctttgttcag cgcggtactg
     4861 gcctgtaaca ttaccgcccg agtttgcgct aatgccgcac gttgctgatt gctctgctcg
     4921 ccgtgattca gtcgttgcaa atcgtcgcga aatgcccaaa aagacatgcc gttactgcca
     4981 atctgcaaga tcccgcagag aatcaaaatt aaaaacagcg tggtcgaaat tcgaatacga
     5041 ttaaacatca acgctcccca tcaggcggca atgaccgcgt tagtaaaaac tcgtcaaaat
     5101 gtttcccagt ttggatcttg ttcagtaatt cgcagtcgtg gctgtgccgg tggttgctca
     5161 ctggcaggac gggatggtgt ttgcggttta ttggtgagtg ggctggctgc cagacggaac
     5221 gcggaaaccg cttgcgttaa acgactcgcc tgttcttcca gcgcagctgc ggcggcagct
     5281 gattcctgca ccagcgatgc gttctgttgc gtgacgcgat ccatttccga aaccgccaat
     5341 gcgacttgat cgatgccacg gctctgttca tccgatgccg atgcaatctc gcccataatg
     5401 tcagtcacgc gagtgacagc attgacgata ttgttcattg tttccccggc gctttcgacc
     5461 agcaccgaac cggtatcaat gcgcgagacg gagtcttcaa tgagggcttt gatctctttt
     5521 gccgccaggg cgctgcgact ggcaagatta cgcacttcac ccgccaccac ggcaaaacca
     5581 cggccctgtt cacccgcacg cgcggcttca accgcggcat tcagcgcgag gatattagtc
     5641 tggaaggcaa taccgtcgat aacgctgata atgtcggcaa ttttcttcga actatcggcg
     5701 atctcatgca tcgttttcac tacgccatcc accactttgc cgccgtgctg ggcggtgtcg
     5761 gaggcacttt gcgccagttg cgaggcctgg cgggcgttat cggcgttttg cttcactgtc
     5821 gcggtgagct gctccatgct ggcggcagtt tcttccagcg cggatgcctg ctgttcagta
     5881 cgggaggaaa gatcggtgtt gcccgccgca atttcacggg taccggcata gatggcatct
     5941 gaaccttcgc ggacatgagt gacggtgtca gtcaaagagc gttgcatatg tgaaacgctc
     6001 tgcgccaggt cacccatttc actgcgcccg tcaatggtca gggtattcgc caggttacca
     6061 ccggcgattt cgcaaatgtg agcaataatt tttgccagcg gagtaagcaa catacggcga
     6121 atgccgtacc acgccaccag cagaatcaat accaccacca gcgcgataac cgccagttgc
     6181 cactgggcaa atcggtaatc atctgcgttg tcagtgacga tatcgcgata cagtttttca
     6241 ctgctgaggg cgtactgagc aaacgcttcg cccattgcat tttgcattcc ctgggttggc
     6301 tgagcgaaat aagctccagt attgccataa tcaagataat caatcagttc agttaacgct
     6361 gtgtaatagt ttttatattt ttcatcaaaa ttacggctgg tagcgaccat ttcaggtaac
     6421 ggtgccatgc ttttgaattt tttataatgc gtcgcggcct gggccaatgt tttcctggcg
     6481 ctatcgagca attcaacttt ggcgttactt tgttgattgg aggaatccat catcatccgc
     6541 accgctgaac gactcaggtt aatgcgcgtt tgcagcatta aatcccaggt tgacgtcagc
     6601 tcgccctgct gttcccgtaa ttgattggaa accacaaagc tcttctggct atggtgaagg
     6661 gaagaaaaaa acaaactgcc ggaaataagc tgtaacagtg cgaatacccc cagcaccatt
     6721 accaacagcg tgactacgcg gatacggtta atcataaggc accttcctga aaacaagttg
     6781 atctcgttat cggcaagaag gggggaaact ttattgctga tgccacccgc cgcgaaattg
     6841 aaataaaaaa cccgatgcgc agatcatcgg gttcatttca attgaggaaa tcgggagaat
     6901 tacgccactt ctgacgccgc gctatctaac agcgccatct cttcgctgtt cagcagtttt
     6961 tcgatgttca ccagaatcaa catacggtcg cccagtgcgc ccagtccagt gagatattct
     7021 gttgaaagcg tcacggcaaa ctctggtgcc ggacgaattt gctccgccgt caatgaaagc
     7081 acgtctgaaa cgccgtcaac cacgatgccg accacccgct gtccgagatt caggacgata
     7141 actaccgtgt tgtcgttata gtccacatcc acctggctga acttaattcg taagtcaaca
     7201 atcggcacaa taacgccgcg cagattcgtg acgcctttga taaacgctgg cgtgttcgca
     7261 atccgtgtta cctgatcgta gccacggatc tcctgcactt tcaggatatc aataccgtac
     7321 tcttcatcac caagggtaaa taccagaaat tcctggcctg acggctcgct ggccagcttt
     7381 gttacattcg tcataccggt catattgtta cctttttact cattcaggcg gcggtgttcg
     7441 ccatacgttg ttcgcggttt atcgcctgca aggcggagac atcaacaatc agtgccacgc
     7501 tgccgtcgcc aagaatggtc gcagcagaaa tgccggggac tttgcgatag ttactttcaa
     7561 ggtttttaac cacaacctgg tgttgaccaa ttaattgatc caccagcaag gcgtagcggc
     7621 gaccgccact ttgtaagatc accacaattc cctgggtggc ttcggttttc gcgcccgcga
     7681 cgttgaacac tttccacagt tcgacgatgg gcagatattc accccgcact tccagcaccc
     7741 gctcgccgcc ggccagtgga tggagatcgg cttcacgggg ttgcagtgat tccataacag
     7801 cattcagcgg cagaatgaaa acttcatccg caacgcgtac ggacatgccg tcgaggatgg
     7861 ccagcgtcag cggcagtaaa atgcggatcg tagtgccagt accctgcttc gactggattt
     7921 cgacatgacc gcccatctcc tggatattac gtttaacgac gtccatgccg acgccgcgcc
     7981 cggagacgtc ggtgacctgc tctgccgtgg agaagcctgg tgcaaatatc agcattgcaa
     8041 cttcgtcgtc gctcatgttt tcgctgacag tcaaaccttg cgaggccgct tttgccagaa
     8101 ttcgctcacg gtttagcccc gccccatcgt cggtcacttc aatgcaaatg ttgccgccct
     8161 gatgttcggc agacagaatt aaatttccga cgctgttttt acctgcggcg agccgttttt
     8221 ctggcagttc aataccgtga tcgaggctat tgcgtaccag gtgggtcagc gggtcgataa
     8281 tgcgttctat caggctcttg tcgagctcgg tggaactgcc caccagcgtc agttctacct
     8341 gcttgccgag ttttcccgcc agatcacgca ccagccgggg atagcgacta aagacatatt
     8401 ccatcggcat catgcgaatc gacatcactg attcctgcaa atcacgggcg ttacgttgta
     8461 actgccccat gctggtaatc aaatcaccat gattaaccgg gtccagttcg ctggaacgct
     8521 gggcaagcat ggactgggtg ataaccagct cgccgacgag gttaattaat tgatcaacct
     8581 tttctaccgc tacacggatg ctggtggatt cactgctgcg cgtcgttttt tcccgctcca
     8641 cgcggccggt tggcgcttgt tcggctgcca gtttaagcac aggtggggta gctatttttg
     8701 gcgaaacttc tactgtttca aaggtaatct gatcggcttc aatcacaaaa cagagtaccg
     8761 ctgtgatgtc atcttcggcg atatcgcccg gtaatattgc cgagagagaa tccgcccctt
     8821 tcaccacgtc atttaacgtt gtcagatgcc ccagctcttc ttccagcagg tcgacttcgc
     8881 cggccttcag gcgcgaaagg ataattcgtc gcggcaactg actgcgactt tgctcatctt
     8941 gcggttcact tttggcaacc acacttaatc gggtcactgc ggatggcgtt tcgcctttcg
     9001 cttctaatgc cagttgacgc aaggcctggc agatataatc gtagctggcg gcatccggct
     9061 cttgcgactg tttataagcg tcgagctgtt cttgcatgat gtccttcgtt tccaaaaaca
     9121 gattgataat gtcggtgttg agttgcatct cacctcgtct ggcttcatcg agcaggtttt
     9181 ccatcagatg cgtggtttcc tgcaaaacgc tgaagccaaa agttcctgcc cctcctttga
     9241 tcgagtgggc agcccgaaag atggcattca attgttcggc atctggcgct tccggctgca
     9301 aaaccagcag atgctgctcc atatcagcca acagttcgtc cgcttcatca aaaaatgtct
     9361 gataaaaatc gcttatatcg atgctcacgc tgtcacctcg gttcggctga tggcattgtg
     9421 ggaacactga cctgtggtgc aacctcaggt ttttctaggg cgcttactgg ctcattctgg
     9481 ctttcggcgt tttcatgcaa aatggcctgt tcggcttgtt tgttcagtac caacaggctg
     9541 atgcgacggt tgacggcatc atcaggtccg cgatcgctta agcgcatcgt tgccgccatg
     9601 ccgacgacac gtaacacttt gccgctatcc aaccctccga ccatcagttc gcggcgggat
     9661 gcattggccc gatcggcaga aagctcccag ttgctgtaac ctttctcacc gctggcgtag
     9721 ggaaaatcat cggtatgacc tgaaaggcta atacggttgg gaataccgtt cagcacaggc
     9781 gcgatggcgc gcagaatgtc gcgcatatag ggttcgacat cggcactgcc ggttctaaac
     9841 atcgggcgat tctggctatc gatgatctga atacgtagac cttcctggac cagatcgatt
     9901 ttgagatgga gacgtaacgc ccgcagtttc ggatcggact ctatcaactg gtcgagatca
     9961 ccccgcaatt tccgcaatcg actttgctcc atgcgttttt tcagctcttc gatgttcggc
    10021 tgcttattca cttccccctg gctttgggtg taatcatcac caccgccggg aattgggctt
    10081 tcactattgg aaatgcgatc gccgcccgta accgctgtcg ccagtggagt ccggaagtac
    10141 tccgcaatct gaatcaactc ttttgggctg gagatggaga tcagccacat caccagaaaa
    10201 aaggccatca tcgcagtcat aaagtcggca taagcaatct tccacgatcc atgtgctgcc
    10261 ccgtggcttt tggctttgcg tcgtttgacg acaataatcg gatgcgcttg attcttcatg
    10321 cttcctcggt tgtcgtctgt tgttgcggat ttttcaccgc acgcacatgc tcttccagtt
    10381 caataaacga cggacgttcg ctggaataga gcgttttgcg accaaactca acggcgatag
    10441 gcggtgcgta accgttcaga ttagaaagca gagtgacttt gacgcactgc atcattttgc
    10501 tggtttcggc gcttttctga cgtaaaacac tcgctagtgg ggaaataaat ccgtaagcca
    10561 ataaaatacc gaggaaagtc cccaccatcg catgtgcgat aagcgccccc agctcggcgg
    10621 caggacgatc ggctgaacct aacgcgtgaa cgccccccat tacagccgcg acaataccaa
    10681 acgccggaag tgagtccccg accagcgcca gactgttcgc cgggacttct gcctcgcttt
    10741 cgtgcgtctc aatctcttca tccatcaggg cttcgatttc gaaggtgttc atgtgaccgc
    10801 tgataatcag gcgcagataa tcgacgataa aatcaagcat gacgctatcc gcgaggatgc
    10861 gtgggtagct ggcgaagatc tcgctctcac ggggattttc aatatcacgt tccagcgaaa
    10921 acatccccat ctgccgcgat ttcgccatca accgataaag cagagccagc agatccatat
    10981 acattgcttt ggtgtatttg gagcgacgaa acagcaacgg cagcgctttc agcgtgcctt
    11041 taatcgcttt gccattattg ccgacgataa acgacccaat ccctgcaccg gcaataatca
    11101 ccagttcagc gggttgatag agtgctccaa ggcttccacc ggtcatcaaa taaccgccga
    11161 aaactgtacc gagaacaacc aggtaaccta ataagataag cacgacatca tccttccact
    11221 gttgaccatg acaggatgtt cagtcgtcag gcgttaacgc gcgattgggg aaaaaaaaag
    11281 cagcggtacg tcgttaccgc tgctggaatg ttgcgccaca ccgtatcagt taaacagcct
    11341 gtactctctg ttcatccagc agttgtggga taatatcggc aggattctgg gaaagtttac
    11401 gtctttttac tgcccgggat ggcggttgac ataagctgca ggcaaagctg ccaacaggct
    11461 ggtgagcgtg ggtaataaaa ttgccgccgc agcagttgca gctggaaagt tgcagtaatc
    11521 cactttcaac aaaccgcacc aatgtccagg cacgggttaa tgccagcagt ggtccttctt
    11581 ctgcttgtgg gcactgttca aggtataaac ggtaggcttt gatcaccgca tcgacgccat
    11641 tacacaaacc ggttttcagt aaaaactgcc atgcattaca gaacatcgaa gcatgaacgt
    11701 tttgttccca ggtcataaac cagtcggttg agaatggcag catgcctttc ggcggtgggc
    11761 ttccgcgcag ttctttataa agttttatca ggcgtccgcg acttaactgt gtttcgcttt
    11821 ccagcatctg caaacgagcg cccagggtga tcaattccat tgccagctga atatcccgcg
    11881 cttcctgaac aatgcttttt tcactcatga tcaggccctt ttcttgcgca gcgcttcttc
    11941 aggctgatta acatcattca gcaagcgtgt tgagagcatg atgccggtat gaatttgctg
    12001 gagatcgtca acgcgggaat cttgcgtcaa ctgagtaatc gtctggtggc tgtcaaaacg
    12061 gaagtgacaa accagctgat tggtttctgc cagcttaacc atttgcggaa g
//
LOCUS       DADLTY010000129        12101 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.129, whole genome shotgun sequence.
ACCESSION   DADLTY010000129 DADLTY010000000
VERSION     DADLTY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.129"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            112..1167
                     /gene="phoE"
                     /locus_tag="JN334_003001"
     CDS             112..1167
                     /gene="phoE"
                     /locus_tag="JN334_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY7439069.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1206..1607)
                     /locus_tag="JN334_003002"
     CDS             complement(1206..1607)
                     /locus_tag="JN334_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY7439070.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="JN334_003003"
     CDS             complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="JN334_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY7439071.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="JN334_003004"
     CDS             complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="JN334_003004"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY7439072.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3721..5178
                     /gene="pepD"
                     /locus_tag="JN334_003005"
     CDS             3721..5178
                     /gene="pepD"
                     /locus_tag="JN334_003005"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY7439073.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5235..5795)
                     /locus_tag="JN334_003006"
                     /pseudo
     CDS             complement(5235..5795)
                     /locus_tag="JN334_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570718.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5911..6954)
                     /locus_tag="JN334_003007"
     CDS             complement(5911..6954)
                     /locus_tag="JN334_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAY7439074.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            complement(7200..7652)
                     /locus_tag="JN334_003008"
     CDS             complement(7200..7652)
                     /locus_tag="JN334_003008"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7439075.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="JN334_003009"
     CDS             complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="JN334_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HAY7439076.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="JN334_003010"
     CDS             complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="JN334_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HAY7439077.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="JN334_003011"
     CDS             complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="JN334_003011"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAY7439078.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="JN334_003012"
     CDS             complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="JN334_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAY7439079.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            10264..12003
                     /gene="flhA"
                     /locus_tag="JN334_003013"
     CDS             10264..12003
                     /gene="flhA"
                     /locus_tag="JN334_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HAY7439080.1"
                     /translation="MLSRSDLLTLLTINFIVVTKEAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLSGGVC
                     IGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDVRH
                     QLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEKSL
                     ETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVIS
                     DGNGVLLPEIRIRENFRLKPSQYAIFINGIEADEADIPADKLMALPSSETYGEIDGVL
                     GNDPAYGIPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQ
                     LHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSTVTKD
                     HILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQAGKVMLDSVP
                     VDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLPRYARLFAPGLHVLSYNEV
                     PDELELKIMGALM"
BASE COUNT         2991 a         3140 c         3029 g         2941 t
ORIGIN      
        1 aatatcatta atctgtaata tatctttaac aatctcaggt taaaaacttt cctgttttca
       61 acggggctct cccgctgaat attcgcgcgt taattaaaat caggaatgaa aatgaaaaag
      121 agcactctgg cattagtggt gatgggcatt gtggcatctg catctgtaca ggctgcagaa
      181 atatataata aagacggtaa taaactggat gtctatggca aagttaaagc catgcattat
      241 atgagtgata acgccagtaa agatggcgac cagagttata tccgttttgg ttttaaaggt
      301 gaaactcaaa ttaacgatca actgactggt tatggtcggt gggaagcgga gtttgccgga
      361 aataaagcgg agagtgatac tgcacagcaa aaaacgcgtc tcgcttttgc cgggttgaaa
      421 tataaagatt tgggttcttt cgattatggt cgtaacctgg gcgcgttgta tgacgtggaa
      481 gcctggaccg atatgttccc ggaatttggt ggcgattcct cggcgcagac cgacaacttt
      541 atgaccaaac gcgccagcgg tctggcgacg tatcggaaca ccgacttctt cggcgttatc
      601 gatggcctga acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga cgttaaaaag
      661 caaaacggcg atggcttcgg cacgtcattg acatatgact ttggcggcag cgatttcgcc
      721 attagtgggg cctataccaa ctcagatcgc accaacgagc agaacctgca aagccgtggc
      781 acaggcaagc gtgcagaagc atgggcaaca ggtctgaaat acgatgccaa taatatttat
      841 ctggcaactt tctattctga aacacgcaaa atgacgccaa taactggcgg ctttgccaat
      901 aagacacaga actttgaagc ggtcgctcaa taccagtttg actttggtct gcgtccatcg
      961 ctgggttatg tcttatcgaa agggaaagat attgagggca tcggtgatga agatctggtc
     1021 aattatatcg acgtcggtgc tacgtattat ttcaacaaaa atatgtcagc gtttgttgat
     1081 tataaaatca accaactgga tagcgataat aagctgaata ttaataatga tgatactgtc
     1141 gcggttggga tgacgtatca gttttaatga atattgccgg atgtgatgca tccggcacat
     1201 ttcactcacg ccgttaactt caccggttca tcacgaaaat cattcgccgg ttccagcttc
     1261 agattcagcg tcgtcagcag ttcacgcagc ttctcgtgaa actcacgcag ggtgtgctcc
     1321 agtcgttcaa ccacttcagt atcttttacc ggaacactct tccagtcgcc tgctttatcg
     1381 aacagaccaa actggtagct gtaggtaaaa cgggattcct gcgcttccag ctccatccac
     1441 cagccccaga attcacgcac ttccggtgcc ggtttcacgt tgacgcatac agccagacaa
     1501 tcgaaaaaga atcgattatc ttcgcactta ccttcacgaa tatacgggcc tagtgcggta
     1561 aattttttga tcaatctgct cttcgggtgt ccactcggta acgtcattgc tatctcctgt
     1621 tgtgatgcaa ctgttttacc aaattggcaa aatttagcaa atttttaaca caagcgtttt
     1681 tcgatccagc cggtgatttc ctgaagacct ttgtcaaaat tccgatacac cgggttaaat
     1741 gggatctcta ataatttacc gtcagcagat gatgaggtga ttaagcgtga gtcctcttcc
     1801 gggctgaacg gatcgttctt ccagtagcct gataacattg gcgttgggca gcgacgcccc
     1861 agcaatcctt gcacttttaa tgaatagcga ttcagctcca cgcgtaacgc ttcgtcggaa
     1921 gcatcatgca tccccaaacg actcgccaga acgtcaagat acatttccgg cacctgttgc
     1981 tggcacttaa aatcactcaa cagggtatga actaccggac caagacaggc aaccgctttc
     2041 agacgcggcg attcaaggta tgccagacgc acggcaacgt tagcgccgaa acggaaacca
     2101 aaggccgcga cgcgagtgtg atccacccac ggtacgttag gcagcgcctt taagacgtgc
     2161 tgatgcaaca ggctggagtc ctgggtgagc ttccattttg aagaaaagcc caccgacggc
     2221 atatcaatag tcagcatcgc aatgccgcgc ggcgcaaaat aacgttcata caggctgtaa
     2281 tagtccgtct gcatcgcatc cagaccacca cacattaata ctgtcgggaa cgggccatcg
     2341 cctttcggca tatgcaaaaa gccggtgatg ggcgcaccgc cgggtacggt aaactccatc
     2401 tgccgcatcg tgcccggcag acgctgggcg gcctcttcat aggcgcggtt tgataaagcc
     2461 tgcgcttgct cggccaggtc atctcctttc agatgaggat aggcggcaat gttgtacaac
     2521 gtagccgcat gcagccagtg tcgaccactg agttgcggat cttcttccgt acaggctttt
     2581 tgttgccaca ccatcgcctg ggtggcccac tcataaatcc agttgccacc acggtagcca
     2641 atcacggtat cgtataaatc atcgtcggta cgttcagcat cgctcatcac aatacgtgcc
     2701 tggacgtcga ggatttcacg tgggtcaata ccgcgccaga tccacatcag acggttaatc
     2761 atgcgatacc agtgagggat ggttttacca tcaagagccg actgcacagg cggttgtgcg
     2821 ccgtgattaa agcggcggac tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac
     2881 agggtttcgc tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact tactggaaac
     2941 tattgtaacc tgcctgaagt taaaaagaac aacgcccggc aatgccaggc gttgaaaaga
     3001 ttagcgaccg gagattggcg ggacgaatac gacgcccata tcccacggct gttcgatcca
     3061 ggtatcttgc gggatatcaa caacatagtc atcaaccagc ggacgaccag ccggttttgc
     3121 gaagatggtg acaaagtgcg cttttggata catttcacga atcgcaacag cagtaccacc
     3181 ggtatccacc aggtcatcaa taacgatgaa gccttcgcca tcgccttctg cgcgtttcag
     3241 cactttaagc tcgcgctggt tgtcgtgatc gtagctggaa atacaaacgg tatcgacatg
     3301 acgaataccc agttcacgcg ccagtaacgc ccccggtacc agaccgccac ggcttacggc
     3361 aataatgcct ttccattgtt cagaaggcat cagtcggctt gcgagtttac gtgcatggat
     3421 ctgcaacatg tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt
     3481 atctacggct taaaaagtgt tcgaggggaa aataggttgc gcgagattat agagatctgg
     3541 cgcactaaaa accagtattt cacatgagtc cgcgtctttt tacgcactgc ctctccctga
     3601 cgcgggataa agtggtattc tcaatacata tctcgcaagc ctgtcttgtg ttgacaacat
     3661 tttctgctaa ccctgtgacc tgcaatactg ttttgcgggt gatcgacaag gagacttaac
     3721 gtgtctgaac tgtctcaatt atctccacag ccgctgtggg atatttttgc caaaatctgt
     3781 tctattcctc acccgtccta tcatgaagag caactcgctg aatacattgt tggttgggca
     3841 aaagagaaag gtttccatgt cgaacgcgat caggtaggta atatcctgat tcgtcaacct
     3901 gctaccgcag gtatggaaaa tcgtaaaccg gtcgtcttac aggcccacct cgatatggtg
     3961 ccgcagaaaa ataacgacac cgtgcatgac ttcacgaaag atcctatcca gccttatatt
     4021 gatggcgaat gggttaaagc gcgcggcacc acgctgggtg cggataacgg cattggtatg
     4081 gcctctgcac tggcggttct ggctgacgaa aacgtggttc acggcccgct ggaagtgctg
     4141 ctgaccatga ccgaagaagc cggtatggac ggtgcgttcg gcttacagag caactggttg
     4201 caggctgata ttctgattaa caccgactcc gaagaagaag gtgaaatcta catgggttgt
     4261 gcggggggta tcgacttcac ctccaacctg catttagatc gtgaagcggt tccagctggt
     4321 tttgaaacct tcaagttaac cttaaaaggt ctgaaaggcg gtcactccgg cggtgaaatc
     4381 cacgttggcc tgggtaatgc caacaaactg ctggtgcgct tcctggcggg tcatgcagaa
     4441 gaactggacc tgcgccttat cgatttcaac ggcggcacac tgcgtaacgc catcccgcgt
     4501 gaagcctttg cgaccattgc tgtcgcagct gataaagtcg acgcactgaa atctctggtg
     4561 aatacctatc aggagatcct gaaaaacgag ctggcagaga aagagaaaaa tctggccttg
     4621 ttgctggact ctgtagcgaa cgataaagct gccctgattg cgaaatctcg cgataccttt
     4681 attcgtctgc tgaacgccac cccgaacggt gtgatccgca attccgacgt ggcaaaaggt
     4741 gtggtcgaaa cctccctgaa cgtcggtgtg gtgaccatga ctgacaataa cgtagaaatt
     4801 cactgcctga tccgttcact aatcgacagc ggtaaagact acgtggtgag catgctggat
     4861 tcgctgggta aactggctgg cgcgaaaacc gaagcgaaag gcgcatatcc tggctggcag
     4921 ccggacgcta attctccggt gatgcatctg gtacgtgaaa cctatcagcg cctgttcaac
     4981 aagacgccga acatccagat tatccacgcg ggcctggaat gtggtctgtt caaaaaaccg
     5041 tatccggaaa tggacatggt ttctatcggg ccaactatca ccggtccaca ctctccggat
     5101 gagcaagttc acatcgaaag cgtaggtcat tactggacac tgctgactga actgctgaaa
     5161 gaaattccgg cgaagtaatt atttgatttg ctgccggatg gcgtttaatc gccttccggc
     5221 agtttcatcc ttcattatcc ttcgataaaa gccatccctg taaacgttcg tcgcgggttg
     5281 ccacgttcaa tctggtgatg gaacattcgc cgctgtgatt tcagcaccgc gctattttcc
     5341 tattgctgct gatccagctt ccaggcaatc agcaatcgcg ccagccgttt gttagcatgc
     5401 tggctgcgct ctgactgaac cttcacgcta ataccggatg ccaaatgcgt ggcgcgtacc
     5461 gccgagtcgg ttttattgac atgttgaccg cccggccccg acgaacgcag cgtctcatac
     5521 cggatcgcat cggattgctc ctgctcatca gcggtaaaac gcccaatgcc cagaaaccag
     5581 tttttacgcc cgtgatgtgg gcgatacggg ctcgggcaaa tccactgaat agtgccacac
     5641 cagctttcgc ttaacgccca tgcgttgtcg ccatccagag aaatcagcgc cgaacgcagc
     5701 gtgtcagagt agcggcctgt ttctgtttcc agcaccgtta ccgcgacgtc ttgtcgggca
     5761 gcttctttaa ttagcctgtc cagcgccttt ttcactgcga gacaacattc ttccggcccc
     5821 tgcgctgagg agagttatag caagatcatc cttttttccc tccactgttt ttgagcgtta
     5881 gcaccggacg cagccgcgcg acaggaataa ttaatccggc ctgccctgac attgcaccac
     5941 actttcagcc gatttatagg cttgtggcgc ttcttcgaag atgagttgtt tatcgcgaca
     6001 aattacccga ctgccaagtt cagtacgcga gagttgcgtc gctgtgtatt tcgctgccag
     6061 acgcccttta cactcggtgc gcccccattt acgcccagcc ccatgcgcca gcgaatgcaa
     6121 tgtttcctca ctcctgacgg gctgaaccag cctggagtaa tcaccgcgtg aaccgggaat
     6181 aaccaccaga ccacagtcat caggcgtggc acctttacga tgtaagaccc cttgttgatc
     6241 accgattcga cacgcgctaa caaagttatg cgccacatcc agaaccggac ttccggtggc
     6301 cttaacctgt tgcattatgc gcaaagcgat caggtggcga ttaaaacgcg caaacgccag
     6361 cgcgtcgtca ctgccttcag gcaaaccatg atgcgaaaac gaggcaatat gccgctgtaa
     6421 aatagactgc cctagccccc tcgagccgct atgaaccagc agttgcagat gctgcgcatc
     6481 cagacctgcc agtgcaaaca gttcagcgtt gatgatttga tcaacttgtt gcagttcagc
     6541 gaaatgattg ccaccaccga tagagccaag cgagctgcgc cacggatgct gtgcaaatgc
     6601 tgacggcagg ttttcctcca gccagctttc ttcagcaacg tcatccaaag ctgataatcg
     6661 cttttcaaac ttatcggcgt tgtatttgcg agcgagaata tctgtttgcc atagcgccat
     6721 gccacagccg atatcattgc cgaccagtgc tgggtaaaaa cggcctgcgg agaagaacgc
     6781 tgcgccaatc gggtagccgc gtccggggtg taaatctggc atccccacta cgcgctgcat
     6841 gttgggtaaa tttgccgtgg tgtgtaattg ttggatcgct aaactctcga tccacaggtc
     6901 atcagatgcg atggtaaata ccgcatcgga taagggacga atataattgc ccatatacca
     6961 ttccgtaaat agactcgtca tacttcaagt tgcatgtgct gcgacttgaa ttattgagag
     7021 taacaattaa aaaatcagga aatggcagac gaatccaggg caagaaaaaa agaattaatc
     7081 ctggaaaggt ctgcaaagag tcattgataa aggatttgtc atgatttacc tccttgcagt
     7141 ttgtggaata agaaaacgcg cacatactag gcgggcgtta ttttcattgc aagctggatt
     7201 taatgttgcg gtttataacg catataaaaa ttaataaacc acgctccccg gcattcaacg
     7261 cgctgctgct taactgtctg aaaaccataa cgttcaaaaa agggttttgc ggttatgctt
     7321 gcgtccaccg taagttcgga ttcagactta ataaaaggtt ttaacaaagc gctggcaacc
     7381 ccacggcggg tgtattcagg gtcaacaaat aacatatcga tatagtgttc aacgcaggta
     7441 ataaagccga ccagttttgc attaataact gcaacccgca cttgtgattt cgcgagtttc
     7501 tccttccagc gagattcgtc aatctgcgcc caggcggcaa tttgttgtag tgaataatgc
     7561 tgactgacgg tcatcgtaac cgctctaatg aaaatagcgc atagttgctg aaaatcgcca
     7621 ggctgataat ctcttatttg tatgttattc ataatataaa ttcaaaaacg catgcgaaac
     7681 gcttctgcca tttttccaat tttatgcatt tggttgttat ctcgagccag tttatgaggt
     7741 tcaggcttca gaatggcaat gagcaaccat gcttgctcat caaacgcacc ctgacaatat
     7801 accaaatgcg cttcgtcatt cgttctgctg aattggcgca actgtggcgg aaatggatta
     7861 ttcacatttg ccagatgaat atgagcaact cgctcaaatt tgattaatgg ccaggtaaac
     7921 gagtcgtcgt agagtgcatc gcgaccaaat atatctggca aaacaccgtc acgcttatag
     7981 gaaataaaat ccgccgttaa cgcatcaagt tcctctgctg taagttgcag gcgaataagt
     8041 tttgttttga atacccgcat ccttattcct taaagtcatt gaaatcatca tccgtcatat
     8101 cattaagata ttgtttagcg cggcgtgtaa tttcctctaa tcttgcagca cttggacgaa
     8161 agcgcgcctt tatgggcttt ttctcctctt gttcagcaag catgtccatt aacgcttcga
     8221 atgcgctggc acttaagaga tatcctgcgg ggcgattatt agaaagaacc gcaactggtt
     8281 gatcaataaa gtatttagct gggtttttac gtaactcagt gatattgacc gatttttcag
     8341 cgagaattcg atgcatacag tgataccctc ataataatgc acaccagaat atacataata
     8401 gtatacatca taatcccagc accagttgtc tttccatttg cgggtcaagc aacgtcacat
     8461 gcagccccac cagacgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     8521 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgggtg gtctgctgaa
     8581 aatcgtcgaa ctttaatttc accccctggc gagcaatcag taaatcaggt tttacctttg
     8641 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     8701 gatgaatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgtaac cgttcgctgt
     8761 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     8821 agcgtttaag cagaatcacc agatcacact tttgtacatc accgcaggtc cgcagcccca
     8881 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gattttttcc agtggtaagg
     8941 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctgaccgttg ggtttattca
     9001 tgtcggaggc gattttggcg agaaacttta ctggtgccac gcccgcagat gccgtcagtt
     9061 gcagctcgtt gaagatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     9121 aatggacgct atcggtgaca tcgagataag cctcatccag tgacaacggt tcaatgcgcg
     9181 aggtgtagcg cgagaagatt tcacggatat gatttgaggc ttctttgtag gcgtcaaagc
     9241 gccccggaag caaggtgaga tgtgggcata atttgagcgc catcccagta ggcatagcgc
     9301 tacgtacgcc aaatttacgc gcgggataat tggcggtgct gatcaccccc cgacgttcgc
     9361 ggctgccgcc aatagcaata gggatatcgc gcagagcggg attgtcgcgc atctccaccg
     9421 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     9481 tggtaaagta tacagtgatt tcaggttttg agaaatgcgt aaagattcag catttagcgc
     9541 attcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     9601 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     9661 atctcaatac gccgattgcc cgcgctttgc ggattcttcg cgtccagcag catctggtcc
     9721 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     9781 cgagctgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtag
     9841 gccatcgcat cggtatgccc ggtaataata attttgttat agagcgagtc gaacactggc
     9901 gcgagctcca ccagcaaagt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     9961 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
    10021 tccagattcg cttccatatg cgcatcacgg gcaatggtgt tgatactggt tgccaactcg
    10081 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattcac atcacgcgct
    10141 tttttctccg tctcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
    10201 attttattta acggcgacag cccaccaccg ttaaaaatcg actgcccgtg cagcgcggcg
    10261 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat cgtcgtcacc
    10321 aaagaggccg agcgtatttc cgaggtttct gcccgcttta ccctggacgc gatgcccggc
    10381 aaacagatgg cgattgacgc cgatcttaac gccggattga tcaaccaggc gcaggcgcaa
    10441 acccggcgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
    10501 aagtttgtac gcggagacgc catcgccggg atgatgattc tggcgatcaa cctgagcggc
    10561 ggcgtctgta tcggcatctt caaatacaac ctgagcgccg acgccgcctt ccagcaatac
    10621 gtgctgatga ccatcggcga cggcctggtg gcgcagatcc cttccctgct gctctccacc
    10681 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgc
    10741 caccagttgc tggcaagccc gtcggtgctc tacaccgcca ccgggatcat gttcgtgctg
    10801 gcggtggtgc cggggatgcc gcatctgccg tttttgctgt tcagcgccct gcttggcttt
    10861 accggctggc ggatgagcaa acgcccgcag gcggcggagg cggaagagaa aagcctcgaa
    10921 acgctgaccc gcaccatcac tgaaaccagc gaacaacagg tcagttggga aactattccg
    10981 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcg
    11041 cagggcaacc cgctcaccca gcggattcgc ggcgttcggc aggtgatttc cgacggtaac
    11101 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gcctcaagcc cagccagtac
    11161 gctattttca tcaacggcat tgaggctgat gaagcggata ttccggcgga taaactgatg
    11221 gcgctgccct ccagcgaaac ctacggcgag attgacggcg tgctggggaa cgacccggcg
    11281 tacgggatac cggtcacctg gatccagcct gcgcagaaag cgaaggctct gaatatgggg
    11341 tatcaggtga tcgacagcgc cagtgtgatt gccacccatg tgaacaagat tgtgcgcagc
    11401 tatattcctg atttgtttaa ctatgatgac atcacgcagt tgcataaccg tttgtcgtcg
    11461 atggcaccgc gcctggcgga ggatttaagc gcggcgctca attacagcca attgctgaaa
    11521 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
    11581 gtgctggtcg ccagtagcac ggtgactaaa gatcatattc tgctggcggc cgatgtgcgc
    11641 ctggcgctgc ggcgcagcat tactcatccg ttcgttcgca agcaggagct gacggtgtat
    11701 acgctgaata atgagctgga aaatctgctg accaacgtag tgaatcaggc gcaacaggcc
    11761 ggcaaagtga tgctcgacag cgtgccggtt gacccgaata tgctcaacca gttccagagc
    11821 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagatccggt gctgctggtg
    11881 ccgccgcagc tacgcccttt gctgccgcgt tatgcaaggt tgtttgcgcc ggggctgcat
    11941 gtgctgtcgt ataacgaagt gccggatgag ctggagttga agattatggg ggcgttgatg
    12001 taagaatccg gcatcggtgc acaattgccg gatgcggcgt aaacgcctta tccggcctac
    12061 cgttcaggca caagttttta ggcatgataa gacgcgccag c
//
LOCUS       DADLTY010000130        12086 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.130, whole genome shotgun sequence.
ACCESSION   DADLTY010000130 DADLTY010000000
VERSION     DADLTY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.130"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(313..930)
                     /gene="tdk"
                     /locus_tag="JN334_003014"
     CDS             complement(313..930)
                     /gene="tdk"
                     /locus_tag="JN334_003014"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAY7439081.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1535..1948
                     /gene="hns"
                     /locus_tag="JN334_003015"
     CDS             1535..1948
                     /gene="hns"
                     /locus_tag="JN334_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HAY7439082.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2092..3000)
                     /gene="galU"
                     /locus_tag="JN334_003016"
     CDS             complement(2092..3000)
                     /gene="galU"
                     /locus_tag="JN334_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAY7439083.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3202..4215)
                     /gene="rssB"
                     /locus_tag="JN334_003017"
     CDS             complement(3202..4215)
                     /gene="rssB"
                     /locus_tag="JN334_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HAY7439084.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4307..5212)
                     /gene="rssA"
                     /locus_tag="JN334_003018"
     CDS             complement(4307..5212)
                     /gene="rssA"
                     /locus_tag="JN334_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAY7439085.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5325..5783
                     /locus_tag="JN334_003019"
     CDS             5325..5783
                     /locus_tag="JN334_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HAY7439086.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5833..6675
                     /gene="purU"
                     /locus_tag="JN334_003020"
     CDS             5833..6675
                     /gene="purU"
                     /locus_tag="JN334_003020"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HAY7439087.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6835..6919
                     /locus_tag="JN334_003021"
     tRNA            6835..6919
                     /locus_tag="JN334_003021"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6869..6871,aa:Tyr,seq:gta)
     gene            6966..7097
                     /locus_tag="JN334_003022"
     ncRNA           6966..7097
                     /ncRNA_class="other"
                     /locus_tag="JN334_003022"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7281..7958)
                     /gene="narI"
                     /locus_tag="JN334_003023"
     CDS             complement(7281..7958)
                     /gene="narI"
                     /locus_tag="JN334_003023"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAY7439088.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7958..8668)
                     /gene="narJ"
                     /locus_tag="JN334_003024"
     CDS             complement(7958..8668)
                     /gene="narJ"
                     /locus_tag="JN334_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAY7439089.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8665..10203)
                     /gene="narH"
                     /locus_tag="JN334_003025"
     CDS             complement(8665..10203)
                     /gene="narH"
                     /locus_tag="JN334_003025"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY7439090.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10200..>12086)
                     /locus_tag="JN334_003026"
     CDS             complement(10200..>12086)
                     /locus_tag="JN334_003026"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY7439091.1"
                     /translation="RYETVTAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLG
                     TNPLTIAGEAEKAGMNPVDYTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSS
                     GKGHEFMLKYLLGTEHGIQGKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSST
                     CLYSDIILPTATWYEKDDMNTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEV
                     CVGYLGKETDIVTLPIQHDSAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPA
                     TYERFTSIGPLMEKIGNGGKGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAA
                     EMILTLAPETNGQVAVKAWAALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSP
                     TWSGLEDEHVSYNAGYTNVHELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTR
                     SVKEVIGQKSNGNPEKALNFLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKD
                     LGIADNDWIEVFNSNGALTARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIH
                     NSVTRITPKPTHMIGGYAHLAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQ
                     ESVK"
BASE COUNT         3029 a         3183 c         2834 g         3040 t
ORIGIN      
        1 ccgaaaaaga gccagtcagt tgcttaactg actggcatta ccccttctgg ggccgttttt
       61 ttgtttaccc aaagcaactt ttccataaac cgacagcatt agccttcatc atatttgcga
      121 cgatgtataa cgcctaaaca cagggatatt gtactttaca ggtcacaagt caacgtcggt
      181 gcttaagagc cctgtgaggc gtatagcggc gttaaaaaac tgctgagaag ggtatatagc
      241 ccggaagaag tgcgtaaaac gaactgacag gataaaagtg ccctgctcac cctgtcagta
      301 aagaaattct tattaatcgt ggcgatgcct ttcctgaata gccgttaatg agccgacttg
      361 taacgcctct ttatagtgtt tacggcatac agaaacgtat cgttcattac caccaattac
      421 cacctgctca ccttcgttat aaggtctgcc tgcttgatca agacgcagca ccatgcttgc
      481 tttacggcca caaaaacaga tggtttttaa ttcaaccagt ttgtcggacc atgccagtaa
      541 gtattggctg ccaataaata attcacctcg aaaatcggta cgtaaaccat aacaaagtac
      601 gggtatatcg agttgatcga caacctccga taattcatat acttgttgtc tggttaaaaa
      661 ctggcattca tcaaccagta cgcaatgaat tgcctgctgt tcatgttccg cacgaatctc
      721 atcaaataat gatgaatttt ggttaaataa ttttgcaggc gatgacaaac ctatacgcga
      781 actgactttc ccggcaccaa agcgatcatc aatttctgcc gtatatacga cagtgcgcat
      841 gccgcgttcc tggtaattgt atgaagattg caacaatgct gtagacttac ccgcattcat
      901 tgcggaatag tagaaatata gctgtgccat cagccacagg ccctcaatga tatgaaataa
      961 agttggctgg agtttatcat aattcgtcag tttttcagct caacagtatg cacagaagtg
     1021 ggtatatgcg ttctccctta cgaagccttg cataatcctt ctgagctatc attacaactg
     1081 cctcgcttgt tataagcggg taaatgactg ctggtaacta ttcacaatct ttaacctgtt
     1141 gcgcaagtaa tagccctctg ttgacctcca ggagatagtg caatactaag tccatgctct
     1201 tattgcgact gttctacttt tcatcattcg cttaataggg aattctcgta aacacaacta
     1261 atacagaaga ctgaaaggtc gtcagcctac gataatctcc ccataaaatg tgacatgaat
     1321 caggaagttt taacctcacg tgctgcgaaa tcatcggtgt aaatagggct atatgccgcg
     1381 tcttttctgg ctaattttat gaaaagatat ttattggcgg cacaaaataa agaacaattt
     1441 tgaattcctt acattcctgg ctattgcaca actgaattta aggctctatt attacctcaa
     1501 caaaccaccc caatataagt ttgagattac tacaatgagc gaagcactta aaattctgaa
     1561 caacatccgt actcttcgtg cgcaggcaag agaatgtaca cttgaaacgc tggaagaaat
     1621 gctggaaaaa ttagaagttg ttgttaacga acgtcgcgaa gaagaaagcg cggctgctgc
     1681 tgaagttgaa gagcgcactc gtaagctgca gcaatatcgc gaaatgctga tcgctgacgg
     1741 tattgacccg aacgaactgc tgaatagcct tgctgccgtt aaatctggca ccaaagctaa
     1801 acgtgctcag cgtccggcaa aatatagcta cgttgacgaa aacggcgaaa ctaaaacctg
     1861 gactggccaa ggccgtactc cagctgtaat caaaaaagca atggatgagc aaggtaaatc
     1921 cctcgacgat ttcctgatca agcaataatc ttttgtagat tgcacttgct taaaatcccg
     1981 ccagcggcgg gattttttat tgtccggttt aagacaattt aataagaata agctataaaa
     2041 aaacggcgtc gattgctcaa cgccgtttcg tggataacac cgatacggat gttacttctt
     2101 aatgcccatc tcttcttcaa gccaggcttt aaattccgtg ccaagggtgt tatgacgaat
     2161 accgtattca acgaaggcct gcatgtaacc taatttatta ccgcagtcat ggctcttccc
     2221 tttcatatga taggcttcca ccgtttcttt ttcgatcagc atatcaattg cgtcggtgag
     2281 ctgaatttca tcaccagctc ccggaggggt ttttgccagc aacggccaaa tatccgcgct
     2341 aagtacgtaa cgacccacaa tagcgagatt agacggcgca acatccgctt tcggtttttc
     2401 taccacacca accatcggta cgctttcacc cggcgctaat tcaacgcctt tgcaatccac
     2461 aacgccatat gcggtcacat cagcaaccgg ttcaaccatg atctggctat gacccgtttc
     2521 atcaaagcgg cggatcatct ctgccaggtt atcctgtgac aaatcggatt catattcatc
     2581 cagaataaca tcaggcaaaa taacagctac cggttcatca cccactaccg ggtgagcaca
     2641 caataccgcg tgtcccaggc ctttcgccag accctgacga acttgcataa tagtcacgtg
     2701 cggtggacaa atagactgca cttcatcaag cagttgacgt tttacacgtt tttccagcat
     2761 tgcttccagt tcaaaactgg tatcaaagtg gttttcaata gagtttttag atgagtgtgt
     2821 aaccagcaca atttcagtaa tgccagccgc aatacattca ttcacgacgt attgaattaa
     2881 tggcttatcg acaagtggca gcatctcttt cgggatggct ttcgtcgccg gcaacatcct
     2941 ggttcctaat cccgcaacgg ggataacggc ttttttgact ttcgtattaa tggcagccat
     3001 ttaaattctc ctggactgtt cgtgttttga acgtgttcat atttctgtat cgcatcccag
     3061 tatatcagca ccccgaaggg gctaagttct gaaaaggagg cgagatagca taattaagac
     3121 aattacttat acgaatggcg acgatagtac caccagccga cattagcagg taatgcaaat
     3181 ttagcccgcg ttatcgtttg ctcattctgc agacaacatc aagcgcagtc gaccaccggt
     3241 tccccatatt tggcattgcc aggcatcgca tcgctggctc aattgattca aataagcgtt
     3301 acctaaagtg cctaacggaa caccattact gatttgcacc tggtgttcgc cggtatttaa
     3361 cgtcgcattc agacccgcag aaaccagaat gagatttttc agttcgcgat gataatagcc
     3421 aactaatagc ggaaactgcc ccggcagatt ggcctgacga agtaaatggt ttacctgctt
     3481 caataacgcg cccaactctg gcaaccgttg attttggtgt gcaagctgtt cctgtaataa
     3541 tccgttaaac aatgcgcgta ataacaaggc agcaagtacg ccattatgtc cagctcgggt
     3601 gacatcaagg caataaaatg ccagatcgtt ttccgaaagt gcggcaatat caagcaccag
     3661 gccgggtttg tccgcggcaa ccaattgacg gtaattaacc cggcaatggg aaatcacctg
     3721 ctgaaccggc ggttgtagtt cctgtaataa tttcgccgct gcggcagggt tatcaaccat
     3781 tgcatcccag tcgcgaaaaa gcctttcctc ttcctcaacg cgcgaattaa acatgctggg
     3841 atagagacag gcaaaaacca tctcgcgcaa gcgattcaga tctttaactg gtttcagcaa
     3901 aacatcttca acgcccagac gtaacgcttt ggcaatatct gccatatttt cagtggcaga
     3961 tatcaccaga actggggtct ggtcgcctct gttacgtata tgctccagca gtttaagccc
     4021 gttcattcgt ggcatcgcga tatcacatat catcaggtct ggagtgaaac ctcccagcaa
     4081 ctcaagggca tccaccccat cagccgccag taccgttgtc gctcccaatg aggaaaacca
     4141 tgaatccaga agcgagcgaa atacctgctc atcttcaaca atgagaatct gttttccgac
     4201 caatggctgc gtcatgttct ctcccctgac tggctttact caatagtggc atgctattgc
     4261 cacctgcgcc tgtcagaatt aacttaagtg tagataaaaa ttctggtcaa atgttggtgc
     4321 gtaccaacgg caaaagttcg tccattttcc tttccactgc cagctgtccg gccgcaatgg
     4381 cagcgtgcgc gcgatggaaa tcaagcgtag atatttgcgg gcaaacaggt tgaatcagaa
     4441 tatcgggcgg atcacctgcc atgcggttcc ttttaaggcg gttctccaac acctggatag
     4501 acgtggtcat aatctctgtt gccgttggcg ctgtcacagc ccgacgtgtc gttatgctgc
     4561 ctaacctttc tttcagacgc gcatgccacg gtagagaatc accattctcg ctattttctt
     4621 cactgacatt aaaggagagc agatcttgtt gcatcaaatg agcatcgtgc tgcaggtcga
     4681 ccgctatcac aatatcagcc cccaatgcac gcgtaagaga aataggaatt gggttaacta
     4741 ctgccccatc aaccagccag tagccgttat gtgcaacggg tgccatgagt cctggaatac
     4801 tgcatgatgc gcgaatagca agatggagat cgccttcagt aaaccataat tcacgccccg
     4861 tacttaaatt ggtggcaaca gccgcaaagc gacgggaaca attttcgatc tctgtttccg
     4921 gcattatttc gcgatattga ttgaagacac gctcgccgcg cagtaaccca ccgcgctgcc
     4981 aggagaggtc catcaggcgt aaaacgtccc aataactgaa agaggttacc caatcttcca
     5041 gcgcagataa tcgatcgcat gcataggccg cgcccaccag cgaaccaatt gaacatcctg
     5101 caacgatatc aatttcaata cccacttttt ttagcgcatt aataacgcca atatgcgacc
     5161 aacctctcgc cgcgccagat cccagcgcca gccctatttt tatctttctc attattcccg
     5221 caagattccc ctggaatgca atcgtagcca catcgctact gctcggttaa catagtgcta
     5281 ctcaggcggt taatacgccg ttattgtttc cagggagatt atttgtgtct cagctttgtc
     5341 cctgtggtag tgctgtcgag tatagcctat gttgccaccc ttatgtgtct ggtgaaaagg
     5401 ttgcacctga tccagaacat ctcatgcgtt cgcgttactg cgcttttgtg atgcaagacg
     5461 ctgattattt aataaagacc tggcatccct cttgtggagc agcggcgtta cgtgccgaat
     5521 tgatgaccgg atttgcgcat actgaatggt tgggattgac cgtatttgaa cattgttggc
     5581 aggacgccga caatatcgga tttgtcagtt ttgttgctcg atttaccgaa ggtggcaaaa
     5641 caggtgcaat aattgaacgt tcgcgatttt tgaaagaaaa cggtcaatgg tactatattg
     5701 acggtacacg tccgcagttt ggtcgcaacg atccctgccc ttgtggttca ggtaaaaaat
     5761 ttaaaaagtg ctgcggccaa taatggttga cggtacggtt tagcaaacac tctcaacaag
     5821 gtttttccag caatgcattc actccaacgt aaagttctgc gtactatttg tccggaccaa
     5881 aaaggtctga tcgcacgtat taccaatatt tgctacaagc acgagttaaa tatcgtacag
     5941 aacaatgaat ttgttgatca ccgtaccggg cgctttttta tgcgcacgga actggaaggg
     6001 atttttaatg attccaccct gctggcggat ctcgatagcg cattgccaga aggctccgtg
     6061 cgtgagctga atcctgccgg tcgtcgccgg atagtgattc tggtcactaa agaagcgcat
     6121 tgccttggcg atttgttgat gaaagccaat tacggcggtc tggatgtcga aatcgcggca
     6181 gttattggta accacgatac tttacgttct ctggttgagc gttttgatat tccgtttgag
     6241 ctggtaagcc atgaagggtt aagccgcaac gagcacgatc aaaagatggc ggacgccatt
     6301 gatgcttatc aacctgacta tgtggtgctg gcgaagtata tgcgggtatt aacgccggaa
     6361 tttgtggcac gcttcccgaa taaaatcatc aatattcacc attcattcct gcctgcgttt
     6421 atcggcgcac gtccttatca ccaggcctat gaacgtggtg tgaagattat tggcgcaacc
     6481 gctcactatg tgaatgacaa tctggacgaa ggcccaatca tcatgcagga cgttattcat
     6541 gtcgatcata cctacacagc tgaagatatg atgcgcgcag gtcgtgacgt cgagaaaaac
     6601 gtcttaagtc gtgcgctata caaagtactg gcgcaacgcg tctttgttta cggtaatcgg
     6661 acgattattc tttaatcgcc agcaaaaata actggttacc tttaatccgt tacggatgaa
     6721 aattacgcaa ccagttcatt tttctcaacg taacacttta cagcggcgcg tcatttgata
     6781 tgatgcgccc cgcttcccga taagggagca ggctagtaaa aagcattacc ccgtggtggg
     6841 gttcccgagc ggccaaaggg agcagactgt aaatctgccg tcatcgactt cgaaggttcg
     6901 aatccttccc ccaccaccat cactttcaaa agccccggaa ttctcaaacg aatctgcaat
     6961 caaatattct gcccaagcgg ggaaggatga gaagcttcga ccaaggttcg acttgagcgc
     7021 cagcgagaga gcgttgccgc aggcaacgac ccgaagggcg aagcgcgcag cgctgagtaa
     7081 tccttccccc accaccatcg cttccttgaa taatcaaaac ctgttactcc gagctaattt
     7141 tgcccgatgg caccacgttt accgggccta caatatcctc ccctgtaggt cggataagcg
     7201 aagcgcatcc gacaactcac cccgcaccgt gcccataaaa aaaccccgcc gaagcagggt
     7261 ctatgtgaac taaaattcgt ttagtgacga gcgcgcacca gctggtactt acgtgtcaga
     7321 tactccaccg gtacgctcca gatgtgtacc agacgcgaga acgggaacag caggaataac
     7381 gtcatcccca gcaccaggtg cagacggaag ataaacgcca caccatcgag gtgttgagaa
     7441 gcgccaccgt ggaaggtcac caccgactgc gcccagccaa ccagtttcat catctcgcta
     7501 ccgtccatat gctgagcgga gaacggaatg gtcagcaggc ccagcgcgca ctggataacg
     7561 agcagcgaca ggatcaggat atccgctccg gtagtggttg cacgcacgcg tgggctgaac
     7621 atacgacgtt tcagcaaaag cacgccgcca atcagacaca gcacgccgct ggcaccacca
     7681 gcaaacatcg ccattttctg tttcacttca atcggcagcc aggcttcata catccagtgc
     7741 ggcgtcaaca taccgaagaa gtgaccgaca aaaatcccca gaataccgat atggaacagg
     7801 ttcgacgcca ggttcatccc tttgcgatcc agcatctggc tggacgccgc gcgccaggtg
     7861 tactgcccgt agtcataacg cagccagcta ccaatcagga agaccgcccc ggctatgtac
     7921 gggtagatat caaagaagaa catattcagg aattgcatta gtgctgtcct ccggtggtga
     7981 tattcagata ttgcggcgcg acggcaccgg caaagcgacg ctgatgagca gtgattgctg
     8041 aatcaccgca gcctttgtca gcaaagaatt taacctgctc ttcttcccag acagcatcca
     8101 gcgcctgcgg cgtatcatcg cgcgcttcgt cggcaatttt ttccgccact ttgtcgctgt
     8161 cgatagcggt attggccaat ttcagcagca gatcaaacat cacggcataa cggctttcac
     8221 gctgttgcag acgcgcgctc agcaatgcca gaatcggcgc gatatctttc aaaccttcca
     8281 cggcttcgct ttgcggcagc tgcgacaggt actccagata cagcggcaga tggtccggta
     8341 attcgcggct gtttaactgc aagccgtgct gctcgtactg cgccagcagg tccaccatcg
     8401 cctggccgcg gtcacgggat tcaccgtgaa catgttcaaa tagcaacagt gacgtggcac
     8461 ggccacggtc gaacagttcg ctgtactgga cctgggcatc gagcggatcc atcgccgtta
     8521 aatcgcgcag gaaaatgccc agcgcatggg catcttcttt cgacagattt ttcgacgcag
     8581 caatcgcctc aaacatctct tgttgatgct gccataaggc agcatccgga tattcaagga
     8641 gacgcgatac aatcacgagt tcgatcatgg atgcggctcc gttttgctgg tcacatcgat
     8701 ggcatcgata cgacggctgt tgaacagatt gaatttggta tctgagccgt ggcagccatc
     8761 accaaaggta aagccgcagc catttttctc cgggaaggct tcccgtgcca gttcacgatg
     8821 actactcggc accacaaagc gatcttcgta gttagcaatc gccagataac ggtacatctc
     8881 ctgtgcctgg gcttcggtca gaccgacctc ttccagcgca cgggtatcaa ctttaccgtc
     8941 aacagtttca gcacgtttgt aatgacgcat cgccagcata cgtttcagcg cacgcagtac
     9001 cggtttggta tcaccggcgg tcagcagatt cgccagatac tgtaccggaa tacgcaagct
     9061 ttcgacgtct ggcagaattc cgttgctgcc cagctcgccc gcgtctgctg cagactgaat
     9121 cggagacaga ggcggcacgt accagaccat cggcagtgtg cgatattctg gatgcagcgg
     9181 cagcgccagt ttccattcca tcgccatttt ataaaccggc gactgctgtg cagcttcaat
     9241 aacgctcagc ggaataccgt ctttaatcgc ctgctcgatg actttcggat cgttcggatc
     9301 gaggaacacg tccagctgac gctggtagag atctttctcg ttctcggtgc tggctgcacg
     9361 ttcaatcgcg tcggcatcgt acagcagcac gccaagataa cggatacgac cgacacaggt
     9421 ttctgagcac acggtcggct gacccgcttc aatacgcgga tagcagaaga tgcacttctc
     9481 agacttaccg ctcttccagt tgaagtagat ttttttgtac gggcatccgg tgatgcacat
     9541 acgccagcca cggcatttat cctggtcgat caggacgatg ccatcttctt cacgcttgta
     9601 aatcgcaccg ctcgggcagg tcgccacaca tgccgggttc aggcagtgtt cgcacaggcg
     9661 cggcaaatac atcataaagg tgttttcgaa ctggctatac atcgccttct ggatgttgtc
     9721 gaagttcttg tctttcgcca gtttatcaaa ctcaccgccc aggtcatctt cccagttcgg
     9781 ccctttttcg attttcgcca tccgttcccc ggtaatcagc gaacgcggac gggcaatcgg
     9841 ctgcgatttg ctgccttccg gcgcggtatg caggttctga taatcaaaat cgaacggctc
     9901 gtaataatcg tcgatccccg gcagatgcgg gttagcaaag attttaccca gcagcatggc
     9961 tcggttaccc atgcgcggtt gcagtttgcc attgatttta cggatccagc cgcctttgta
    10021 tttttcctgg ttttcccagt cagtcgggaa gccctggccc ggcttggttt ccacgttgtt
    10081 gaaccacgcg tattccacgc cttcacggct ggtccagacg tttttacagg taactgaaca
    10141 ggtgtggcag ccgatgcact tatcgagatt cagcaccatg ccgacttgtg aacgaatttt
    10201 cattttacgc tctcctgtac ctggtcattg ccttcgccat ctaaccagtc aatgttcttc
    10261 atcttacgca ctacaacaaa ctcatcgcgg ttagaaccta cggtgccgta gtagttaaag
    10321 ccgtatgcca gatgggcata gccgccgatc atatgcgtcg gtttcggcgt gatacgggtg
    10381 accgagttat ggataccacc acgctgttgg gtaatttccg aaccaggcag gttaacgata
    10441 cgttcctgcg cgtggtacat catggtcatc cctgccggaa cacgctggct gacaaccgca
    10501 cgggcagtca gtgcaccgtt gctgttgaag acttcaatcc agtcgttatc ggcgataccc
    10561 agatctttgg catcggcttc actcaaccag accaccggac caccgcgacc taaagtcagc
    10621 atcagcaggt tgtcgctgta ggtggagtgg ataccccact tctggtgcgg cgtcaggaag
    10681 ttgagcgctt tttccgggtt gccgttggat ttctggccta tcacttcttt caccgaacgg
    10741 gtgtcgatcg gcggacgata aaccagcagg ctttcaccga aatcacgcat ccactggtga
    10801 tcctgataca gttgctgacg accagagagc gtacgccatg ggatcagctc gtgaacgttg
    10861 gtgtaaccgg cgttgtaaga aacgtgttca tcttccagac cagaccaggt cgggctggag
    10921 ataattttgc gcggctgtgc ctgaatatcg cggaagcgga tcttctcgtc ttctttattc
    10981 agcgccagat gcgtatggtc acgaccggta aattcgctca gggcagccca ggctttcacg
    11041 gctacctgac cgttggtttc cggtgccagc gtcaggatca tctctgccgc atcaattgcg
    11101 gtgttcagca tcggctggcc ttttgccgga ccttccgctt tggtgtagtt gagcttacgc
    11161 agcagatcca tctcgctctg ggtgttccag gcaatccctt taccgccgtt accgattttc
    11221 tccatcagcg ggccgataga ggtaaagcgt tcgtaagttg ccggataatc gcgctctacg
    11281 accataatgt gcggtgcagt tttgcctggg atcaggtcgc attcgccttt tttccagtct
    11341 ttcacatcca gcggctgcgc cagttcagcg gcagagtcgt gctggatagg cagcgtgacg
    11401 atgtcggttt ctttacccag atagccgacg cacacttcgg agaatttctt cgcgatggct
    11461 ttgtagattt cccagtcgct tttcgcttcc caggccggat cgaccgccgc agacagcggg
    11521 tgaataaacg gatgcatatc cgaagtattc atgtcgtctt tttcgtacca ggtcgccgtc
    11581 ggcaaaatga tgtcggaata gagacaggtg ctcgacagac ggaagtccag cgtaaccacc
    11641 agatccagct tgccttccag accattgtcc tgccagtcca cttcttccgg cttcacgccg
    11701 ccctgttgcc ccagatcttt accctggata ccgtgctccg tccccagcag gtacttgagc
    11761 ataaactcat gacctttacc ggaagaaccg agcaggttag aacgccagat gaacaggttg
    11821 cgcgggtggt ttttaccgtt ttctggttgt tctgccgcaa aacggatgga accctctttc
    11881 agggatttca ccgtatagtc caccggattc atcccggctt tttccgcttc gccagcgata
    11941 gtcagcgggt tagtgcctaa ctgcggtgca gacggcagcc agcccatgcg ttccgcacgg
    12001 acgttaaagt cgatcaagtg tccggtatag cgggatttgt ccgccatcgg tgacagcaac
    12061 tcttccgccg tgacggtttc ataacg
//
LOCUS       DADLTY010000131        12078 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.131, whole genome shotgun sequence.
ACCESSION   DADLTY010000131 DADLTY010000000
VERSION     DADLTY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.131"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..164)
                     /locus_tag="JN334_003027"
     CDS             complement(<1..164)
                     /locus_tag="JN334_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7439092.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWP"
     gene            377..1840
                     /gene="bepA"
                     /locus_tag="JN334_003028"
     CDS             377..1840
                     /gene="bepA"
                     /locus_tag="JN334_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HAY7439093.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            1861..2220
                     /gene="arsC"
                     /locus_tag="JN334_003029"
     CDS             1861..2220
                     /gene="arsC"
                     /locus_tag="JN334_003029"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAY7439094.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2353..3099)
                     /gene="hda"
                     /locus_tag="JN334_003030"
     CDS             complement(2353..3099)
                     /gene="hda"
                     /locus_tag="JN334_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HAY7439095.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(3149..4438)
                     /gene="uraA"
                     /locus_tag="JN334_003031"
     CDS             complement(3149..4438)
                     /gene="uraA"
                     /locus_tag="JN334_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAY7439096.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(4524..5150)
                     /gene="upp"
                     /locus_tag="JN334_003032"
     CDS             complement(4524..5150)
                     /gene="upp"
                     /locus_tag="JN334_003032"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAY7439097.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            5474..6511
                     /gene="purM"
                     /locus_tag="JN334_003033"
     CDS             5474..6511
                     /gene="purM"
                     /locus_tag="JN334_003033"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAY7439098.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            6511..7149
                     /gene="purN"
                     /locus_tag="JN334_003034"
     CDS             6511..7149
                     /gene="purN"
                     /locus_tag="JN334_003034"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAY7439099.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            7321..9387
                     /gene="ppk1"
                     /locus_tag="JN334_003035"
     CDS             7321..9387
                     /gene="ppk1"
                     /locus_tag="JN334_003035"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAY7439100.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            9392..10933
                     /gene="ppx"
                     /locus_tag="JN334_003036"
     CDS             9392..10933
                     /gene="ppx"
                     /locus_tag="JN334_003036"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HAY7439101.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(10972..>12078)
                     /gene="pdeF"
                     /locus_tag="JN334_003037"
     CDS             complement(10972..>12078)
                     /gene="pdeF"
                     /locus_tag="JN334_003037"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY7439102.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         3104 a         3094 c         3051 g         2829 t
ORIGIN      
        1 ggccattcca gcaaataagc cagcacaata gccaccagca acggagcaag caggccacta
       61 aagaaaaaga taatgccaaa tccggcaact aaaataacca gcaaggcaat cgcttccggg
      121 tcgctaaaac ggcggcgata ccattgcatc aacatttcga gcataaagac cttccctgaa
      181 cctcaagagc gggattgcga tccgcaattg tatcgaaatg tcacaaaaaa gacttcgctt
      241 tttatgacgg attcaggaaa ctgaaaagtc atttgagtgg gctaatcttc gccgttacac
      301 tcaaaggcgg cgaggtggga acgatatttc acagtatcgg tcaaatgact atcttcagaa
      361 atacaggata gaggttatgt tcaggcagtt gaaaaaaaac ctggttgcaa ccctcattgc
      421 tgctatgacc attggtcagg tagccccggc gtttgccgac agcgcagaca ccttgccgga
      481 tatgggaacc tccgcaggaa gcacgctttc cattggtcag gaaatgcaga tgggcgacta
      541 ttatgtccgc cagctacgcg gcagcgcgcc gttaattaat gacccgctgt taacgcaata
      601 tattaattcg ctggggatgc gtctggtttc gcatgccaat tcggttaaga caccgtttca
      661 tttttttctg atcaacaacg acgaaattaa cgcctttgct ttctttggcg gcaacgtggt
      721 gctgcactct gccctgttcc gttattccga taacgaaagt caactggctt cagttatggc
      781 gcacgaaatc tcccacgtca cccaacgtca cctggcgcga gcgatggaag atcaacaacg
      841 cagcgcgccg ctgacctggg tcggcgcgtt aggttctatt ttactggcga tggccagtcc
      901 gcaggcgggg atggcggcgc tgaccggtac actggcggga acgcgccagg ggatgatcag
      961 tttcacccag caaaatgaac aggaagcgga ccgcattggt attcaggtgc tgcaacgctc
     1021 gggattcgat ccacaggcga tgccgacctt cctggaaaaa ttgctcgatc aggcgcgtta
     1081 ctcctcgcgc ccgccggaaa ttttattgac tcacccgttg ccggaaagtc gtctggcaga
     1141 tgcccgcaac cgtgctaatc agatgcgccc gatggtggtg cagtcgtcgg aagatttcta
     1201 tctggcgaaa gcgcgcacac tggggatgta taattccgga cgtaaccagc tcaccagtga
     1261 tttgctggat gaatgggcga aaggaaacgt tcgtcagcaa cgagcggcgc aatatggtcg
     1321 tgctttacag gcgatggaag ccaataaata cgacgaggcg cgtaaaacgc tgcaaccgtt
     1381 actggcggca gaacctggca acgcatggta tctcgatctg gctaccgata tcgatcttgg
     1441 gcaaaacaaa gccaatgagg caatcaatcg cctgaaaaat gcccgcgatt tacgcaccaa
     1501 tccggtgttg cagctcaacc tggcgaacgc ttatctgcaa ggcggtcaac cacaagaagc
     1561 ggccaatatt ctgaatcgct acacctttaa taataaagat gacagcaacg gctgggattt
     1621 gctggcacag gcggaagccg cgctaaataa ccgcgatcag gagctggctg cgcgagcaga
     1681 aggttatgcg ctcgccggac gactcgatca ggccatttcg ctgttgagta gcgccagttc
     1741 gcaggtgaaa ttaggcagcc tgcaacaagc gcgttacgat gcgcgcatcg accagttgcg
     1801 ccagctgcag aaacgcttta agccttatac caagatgtaa ttcgcattaa aggaagaaca
     1861 atgaccaaac aggtaaaaat ctaccataac ccacgctgtt caaagagccg ggaaacgctg
     1921 aacctgctga aagaaaacgg cgtggagcca gaagtggtgc tctaccttga gacacccgcc
     1981 gatgcggcaa cgctgcgcga tttgctgaaa atgctgggga tgaatagcgc ccgtgaattg
     2041 atgcgccaga aagaggatct ttataaagaa ctaaacctgg ctgacagttc gcttagcgaa
     2101 gaggcgctga ttcaggcaat ggtggataat ccgaagctga tggagcgccc gattatggtc
     2161 gcgaatggca aagcgcggat tggtcgtcca ccggagcagg tactggaaat cgtgggttaa
     2221 gatgtgctcc atcgctagtt gaagcacatt gccggatgcg acgcttgcca cgtcttatcc
     2281 ggcctacgaa tactatggat taacttcgcg taggtcaggc gttcgcgccg catccgacaa
     2341 taaacacctt atctacaact tcagaatttc tttcacaaac ggaatggtca gcttacgttg
     2401 cgcggtaatc gacgcacgat ccaactgatc caacgtcata aatagcgtgc gcatttctct
     2461 gtcgagccgc ttcagcaaga aacgccccac atcttccggc agttcaaaac cacgcaaacg
     2521 cgcgcgtaac tgtagcgcct gcaacttatc ttcatcagaa agtggctgca atttgtagat
     2581 ctgcccccag tcgagacgcg acgcgagatc cggtaatccc agattcaact gccgcggtgg
     2641 acgatcgccg gtgatcaaca accgtgtttt gcccgattcc agaattcgat tgtagagatc
     2701 gaaaatcgcc atctcccaca actcatcgcc tgcaatacac tcaatgttgt cgatacagac
     2761 cagcgacaaa tgctccatac cgtcgagcac ttccggaaca aaccaggtgc gtttatccag
     2821 cgggacatag cccaccgcat cgccacgctg cgacaattcc gcgcaagccg cgtgcagcag
     2881 atggctgcgc cccgcgcctt cgcgtgccca gagatagatg taaccgctat gttcctgacg
     2941 cagcacgttt tgcagcgcgg ccagtaaaga ggagttatcc cccggccaga aacttgcaaa
     3001 ggtttcgtcg tcaggaagat aaagtggcaa agagagctgt gccggtgtgt tcagagatac
     3061 ctcaaccagg atttcacaaa atcgcgagaa gtttaccaca gaatcccatg atgtttgaac
     3121 cgggcagcaa cactgcccgg tcggtacatt atttgtctgt tatatccgcg tcttctgcgt
     3181 ccagcaccac ttcttcaggg cgcagcacac tgatcagttt gaaaatcaaa cttaagccga
     3241 taccgacgat ggtcgccagc gccatacctt ttaactccgc cgcgccgatg tttaccttcg
     3301 cgccactgac gccgatgatc aaaatcacgg aagtcaggat caggttctgt gctttgttgt
     3361 aatccacttt cgattcgatc aaaacacgaa taccggaagc accaatgaca ccataaagca
     3421 gcagcgaaac gccgcccatc accggcaatg ggatcatctg gatagcggca gccagtttac
     3481 cgacgcagga aagcaggata gcgaaaatcg ccgccccgcc gataacccag gtactgtaaa
     3541 cacgggtgat cgccatcacg ccaatgtttt ctccgtaagt ggtatttggc gtagagccaa
     3601 agaagccgga gatcacggtc gacaggccgt tagcaaacat cgaacggtgc agacctggat
     3661 cgcgcagcag atcttttttg acgatattag ccgttactac caggtgccct acgtgttcgg
     3721 caataaccac taacgccgct ggcagaatag tcagaatggc aaaccactcg aagcgcggcg
     3781 tatagagggt tggcagtgca aaccagtgag cattaataat cggcgtggta tcgacaattc
     3841 ccattgcgaa agagagcgcg taccccacca gcacgccaat taaaatcggg ataattgcca
     3901 ggaaaccacg aaacagcacg gaacctaaaa ccgtgaccgc cagggtggta atagagatga
     3961 tgatggtttt ggagtttggc gtttgccctt cagccgggag taaacccgcc ataccggcag
     4021 ctacgcccgc cagctccaga ccgatgacgg caacgattgc gcccattgcc gcaggtggaa
     4081 acagcacgtc caaccagccg gtccccgctt tcttcacgat aaaagaaacc aggcagaaca
     4141 gcacgccgca cataataaag ccgcccagcg cgacttcata ccctaacggc aacagtaaca
     4201 ataccggtga aataaaggca aagctggaac caagataagc cggaattttc cctttacaga
     4261 tgaagagata cagcagcgtt ccaataccgt taaataacag tacagtcgcc gggttaatat
     4321 gaaataagac gggcaccagg acggttgcac caaacatggc gaacaaatgt tgcaaactaa
     4381 gcgggattgt ctgtaaaagt ggcggtcttt cactcacccc gatagcacgg cgcgtcatag
     4441 tattatcctc tgtattatgt gttataggcg ctttactcaa aaaaaagccg actcttaaag
     4501 tcggctttaa ttatttttat tctttatttc gtaccaaaga ttttgtcacc ggcatcgccg
     4561 aggcccggaa taatgtatcc gtgctcgttc agtccctggt caatcgatgc agtatacagt
     4621 tcgacgtccg ggtgcgcttt ttccagcgca gcgatacctt ctggcgcagc gaccagcacc
     4681 agaactttga tgctgctgca gcccgctttt ttcagcaggt cgatggtcgc gataacggaa
     4741 ccaccggttg ccagcattgg gtcaacgatc agcgccatac gctcatcgat gttagaaacc
     4801 agtttctgga agtacggtac cggctccagc gtttcttcat tacggtacat accgacaacg
     4861 ctgatgcgcg cgctcggaac gttttccagc acaccgtcca tcataccaag acccgcacgc
     4921 agaattggca caacggtaat tttcttacct ttgatctggt cgatttctac cgggccgttc
     4981 cagccttcga tagttacttt ttccgtttcg aggtcggcgg tcgcttcgta agtcagcagg
     5041 ctacccactt cggaagcgag ttcgcgaaag cgcttggtgc tgatatcttg ctcacgcatc
     5101 agtcccagct tgtgtttgac gagtgggtgt ttgacttcca cgatcttcat actctttctc
     5161 ctttgagggg cagccacaaa aaaatcgacg gattatacct cctttcttca gggcggcaat
     5221 attattttcg ttgactttag tcaaaatgat aacagtttga gataaagtta ttttatattc
     5281 aaatggttat gaaagaagat tattccatcc gaaaactaac ctttgccctg gcacaagtct
     5341 tctttcgccg cgcgcccggg gaaaagacgt gcaaaaaggt tgtgtaaaac agtctcgcaa
     5401 acgtttgctt tccctgttag aattgcgccg aattttattt ttctaccgca agtaacgcgt
     5461 ggggacccaa gcagtgaccg ataaaacctc tcttagctac aaagatgccg gtgttgatat
     5521 tgacgcgggt aatgctctgg ttggaagaat caaaggcgta gtgaagaaaa cgcgtcgtcc
     5581 ggaagtgatg ggcggtctgg gcggcttcgg tgcgctgtgt gcattgccgc aaaaatatcg
     5641 tgaacccgtg ctggtttccg gcactgacgg cgtaggtacc aagctgcgtc tggcaatgga
     5701 cttaaaacgt cacgacacca ttggtattga tctggtcgcc atgtgcgtta atgacctggt
     5761 ggtgcaaggt gcggaaccgc tgtttttcct cgactattac gcaaccggaa aactggatgt
     5821 tgataccgct tcagcggtga tcagcggcat cgcggaaggt tgtctgcaat cgggctgttc
     5881 tctggtgggt ggcgaaacgg cagaaatgcc ggggatgtat cacggtgaag attacgatgt
     5941 cgcgggtttc tgcgtgggcg tggtagaaaa atcagaaatc atcgacggct ctaaagtcag
     6001 cgacggcgat gtgctgattg cactcggttc cagcggtccg cactcgaacg gctattcgct
     6061 ggtgcgcaaa attcttgaag tcagcggttg tgatccgcaa accaccgaac ttgatggtaa
     6121 gccattagcc gatcatctgc tggcaccgac ccgcatttac gtgaagtcag tgctggagtt
     6181 gattgaaaag gtcgatgtgc atgccattgc gcacctgacc ggcggcggct tctgggaaaa
     6241 cattccgcgc gtattgccag ataataccca ggcagtgatt gatgaatctt cctggcagtg
     6301 gccggaagtg ttcaactggc tgcaaacggc aggtaacgtt gagcgccatg aaatgtatcg
     6361 caccttcaac tgcggcgtcg ggatgattat cgccctgcct gctccggaag tggacaaagc
     6421 cctcgccctg ctaaatgcca acggtgaaaa cgcgtggaaa atcggtatca tcaaagcctc
     6481 tgattccgaa caacgcgtgg ttatcgaata atgaatattg tggtgcttat ttccggcaac
     6541 ggaagtaatt tacaggcaat tattgacgcc tgtaaaacca acaaaattaa aggcaccgta
     6601 cgggcagttt tcagcaataa ggccgacgcg ttcggccttg aacgcgcccg ccaggcgggt
     6661 attgcaacgc atacgctcat cgccagcgcg tttgacagtc gtgaagccta tgaccgggag
     6721 ttgattcatg aaatcgacat gtacgcaccc gatgtggtcg tgctggctgg ttttatgcgc
     6781 attctcagcc cggcgtttgt ctcccactat gccgggcgtt tgctgaacat tcacccttct
     6841 ctgctgccga aatatcccgg attacacacc catcgtcagg cgctggaaaa tggcgatgaa
     6901 gagcacggta catcggtgca tttcgtcacc gatgaactgg acggtggccc ggttatttta
     6961 caggcgaaag tcccggtatt tgctggtgat acggaagatg acatcaccgc ccgcgtgcaa
     7021 acccaggaac acgctattta tccactggtg attagctggt ttgccgatgg tcgtctgaaa
     7081 atgcacgaaa acgccgcgtg gctggatggt caacgtctgc cgccgcaggg ctacgctgcc
     7141 gacgagtaat gcccccgtag ttaaagcgcc agctctgccg ctggcgtttt tcaattcacc
     7201 tgtaaatcgc aagctccagc agtttttttc ccccttttct ggcatagttg gacatctgcc
     7261 aatattgctc gccataatat ccaggcagtg tcccgtgaat aaaacggagt aaaagtggta
     7321 atgggtcagg aaaagctata catcgaaaaa gagctcagtt ggttatcgtt caatgaacgc
     7381 gtgcttcagg aagcggcgga caaatctaac ccgctgattg aaaggatgcg tttcctgggg
     7441 atctattcca ataaccttga tgagttctat aaagtccgct tcgctgaact gaagcgacgc
     7501 atcattatta gcgaagaaca aggctccaac gctcattccc gccatttact gggcaaaatt
     7561 cagtcccggg tgctgaaagc cgatcaggaa ttcgacggcc tctacaacga gctattgctg
     7621 gagatggcgc gcaaccagat cttcctgatt aatgaacgcc agctctccgt caatcaacaa
     7681 aactggctgc gtcattattt taagcagtat ctgcgtcagc acattacgcc gattttaatc
     7741 aatcctgaca ctgacttagt gcagttcctg aaagatgatt acacctatct ggcggtggaa
     7801 attatccgta gcgatagcat ccgttacgcg ctgctggaga tcccatcaga taaagtgccg
     7861 cgctttgtga atttaccgcc agaagcgccg cgtcgacgca agccgatgat tcttctggat
     7921 aacattctgc gttactgcct tgatgatatt ttcaaaggct tctttgatta tgacgcgctg
     7981 aatgcctatt caatgaagat gacccgcgat gccgaatacg atttagtgca tgagatggaa
     8041 gccagcctga tggagttgat gtcttccagt ctcaagcagc gtttaactgc tgagccggtg
     8101 cgttttgttt atcagcgcga tatgcccaat gcgctggttg aagtgttacg cgaaaaactg
     8161 actatttccc gctacgactc catcgtcccc ggcggtcgtt atcataattt taaagacttt
     8221 attaatttcc ccaatgtcgg caaagccaat ctggtgaaca aaccactgcc gcgtttacgc
     8281 catatttggt ttgataaagc ccagttccgc aatggttttg atgccattcg cgaacgcgat
     8341 gtgttgctct attatcctta tcacaccttt gagcatgtgc tggaactgct gcgtcaggct
     8401 tcgttcgacc cgagcgtact ggcgattaaa attaacattt accgcgtggc gaaagattca
     8461 cgcatcatcg actcgatgat ccacgccgca cataacggta agaaagtcac tgtggtggtt
     8521 gagttacagg cgcgtttcga cgaagaagcc aacattcact gggcgaagcg cctgaccgaa
     8581 gcaggcgtgc acgttatctt ctctgcgccg gggctgaaaa ttcacgccaa actgttcctg
     8641 atttcacgta aagaaaacgg tgaagtggtc cgttacgcac acatcgggac cgggaacttt
     8701 aacgaaaaaa ccgcgcgtct ttatactgac tattcgttgc tgaccgcaga tgcgcgtatc
     8761 accaacgaag tacggcgggt atttaacttt attgaaaacc cataccgccc ggtgacattt
     8821 gattatttaa tggtgtcacc gcaaaactct cgccgtctgt tatatgaaat ggtagaccgc
     8881 gaaatcgcca acgcgcagca agggctgccc agtggtatca ccctgaagct aaataacctt
     8941 gtcgataaag gcctggttga tcgtctgtat gcggcctcca gctccggcgt accggttaat
     9001 ctgctggttc gcggaatgtg ttcgctgatc cccaatctgg aaggcattag cgacaacatt
     9061 cgtgccatca gtattgttga ccgttacctt gaacatgacc gggtttatat ttttgaaaat
     9121 ggcggcgata aaaaggtcta cctttcttcc gccgactgga tgacgcgcaa tattgattat
     9181 cgtattgaag tggcgacacc gctgctcgat ccgcgcctga agcagcgggt gctggacatc
     9241 atcgacatat tgttcagcga tacggtcaaa gcacgttata tcgataaaga actcagtaat
     9301 cgctacgttc cccgcggcaa tcgccgcaaa gtacgggcgc agttggcgat ttacgactac
     9361 atcaaatcac tcgaacaacc tgaataaccc tatgccaata cacgataaat cccctcgtcc
     9421 gcaggagttt gctgcggtcg atcttggttc aaacagtttt cacatggtca tagcccgtgt
     9481 ggtagatggt gccatgcaga ttattggccg cctgaaacag cgggtgcatc tggcggacgg
     9541 cctggggcca gataatatgt tgagtgaaga ggcaatgacg cgcggtttga actgtctgtc
     9601 gctgtttgcc gaacggctac aagggttttc tcctgccagc gtctgtatag tcggcaccca
     9661 tacgctgcgt caggcgctga acgccactga ctttctgaaa cgcgcggaaa aggtcattcc
     9721 ctacccgatt gaaattattt ccggtaatga agaagcccgt ctgattttta tgggcgtgga
     9781 acatacccaa ccggaaaaag gtcgcaaact ggttattgat attggcggcg gatctacgga
     9841 actggtgatt ggtgaaaatt tcgaacctat tctcgttgaa agccgccgga tgggttgtgt
     9901 cagctttgcc cagctttatt ttcctggcgg ggtcatcaat aaagagaatt ttcagcgcgc
     9961 tcgcatggcg gcagcacaaa aactggaaac tttaacctgg caattccgta ttcagggctg
    10021 gaacgttgca atgggcgctt ccggtaccat aaaagccgcc catgaagtgt taatggaaat
    10081 gggcgagaaa gacgggataa ttaccccgga acgtctggaa aaactggtaa aagaaatttt
    10141 acgtcaccgt aatttcgcat cgctgagttt accgggtctt tccgaagagc ggaaaacagt
    10201 cttcgttcct ggactggcga ttttatgtgg tgtgtttgat gctttagcca tccgtgaact
    10261 gcgcctttct gacggggcgt tacgcgaagg cgtactgtat gaaatggaag gacgtttccg
    10321 tcatcaggat gtgcgtagtc gcaccgccag cagcctcgcc aaccagtatc acatagacag
    10381 cgaacaggcc cgacgagtgc tggataccac tatgcaaatg tacgaacagt ggcgggaaca
    10441 gcaaccgaag ctggcgcatc cgcaactgga ggcgctactg cgatgggccg ccatgctgca
    10501 tgaggtcggg ttgaatatca accacagcgg tttgcatcgc cactccgctt atattctgca
    10561 aaacagtgac ttgccgggtt ttaatcagga acagcagctg atgatggcga cactggtgcg
    10621 ctatcaccgt aaagcgatta agctcgacga tctgccgcgc tttaccttgt ttaagaagaa
    10681 acagttcctg ccactgatac agctattgcg ccttggcgta ttactaaaca atcaacgtca
    10741 ggcaaccacc acaccgccaa cattgacact gattactgat gacagtcact ggacactgcg
    10801 tttcccgcat gactggttta gtcagaatgc gctggtactg cttgatctgg aaaaggagca
    10861 agaatactgg gaaggcgtgg ctggctggcg gttgaaaatt gaagaagaaa gtaccccaga
    10921 aatcgccgct taatgctttg cgggccgacg agaaatgtcg gcccgcatta ttcaggcact
    10981 ttcgcgaatg ggttcgattt cattcagcgt atcaattaac ggttgcggct taccaataag
    11041 ataaccctgc atataatcga tccccaaaga gagcaccgcc tcgcggatct cttcgttttc
    11101 aacgtactct gccactaccc gcattttctt cattcgcgcc agatggcaaa tcgacgccac
    11161 aatctgataa tccagactat ttgacacaat attgcggata aagctgccgt caattttaag
    11221 cagatcggca ttcacatttt taagccgtgc atagctggcg tagccggtgc cgaaatcatc
    11281 aatcgcaatc tggcagccta attcctgaag atgctgcaag gtgatttgcg cctgcttaac
    11341 attggtcaga gcattacttt cggtgacttc aaaaataagt tgccacgctt caatctgata
    11401 tttagccagc aactgactga cttcaacagg aaaacgagcc tgacataccg aggttggaga
    11461 cagattaata gcaaaacggt gagcgggcat cttcgctctg ttttcagcca taaattgcag
    11521 cgtatgctca atgacccaca tgtcgatact cgacgataaa ccaaattcgt gcgcgaccgg
    11581 caagaagcta ccggggccga tcagttcatc attctcacct ttcatgcgca gaagaatttc
    11641 atggtaaaca tcaccacgca taccggtaat cggctgggcc atcaggaaaa aatggttgtg
    11701 ttccagcgcc tgctgtagtc gattcatcat cgcgacttta tctttcaatt cgcgttgcaa
    11761 atacattgcc ccgcaacgct gcatattttc cggggcgttg gtcacgatgg aaagttcggc
    11821 gaccgtattt agctctccca gcagcaggta gatatgattc actggcgagc gcacatagca
    11881 gtaactgacg ccaatctgcg gttgcatcgg catcccatcc caaaagaaac gaaattgctt
    11941 gagatgacta tccagtgcgg taatgcgctc ctggtgcgat tctgtattca gtcgcagcgc
    12001 gagatcgtta cccgaaagct gataaacatc ttcacccggt tccagcaagg gtgacagcca
    12061 gtgagaaagt ttttgctt
//
LOCUS       DADLTY010000132        12062 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.132, whole genome shotgun sequence.
ACCESSION   DADLTY010000132 DADLTY010000000
VERSION     DADLTY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.132"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="JN334_003038"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JN334_003038"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="JN334_003039"
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="JN334_003039"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HAY7439103.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="JN334_003040"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="JN334_003040"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAY7439104.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="JN334_003041"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="JN334_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..4695)
                     /locus_tag="JN334_003042"
     CDS             complement(3361..4695)
                     /locus_tag="JN334_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7439105.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5070..6641
                     /gene="garD"
                     /locus_tag="JN334_003043"
     CDS             5070..6641
                     /gene="garD"
                     /locus_tag="JN334_003043"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HAY7439106.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRIELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="JN334_003044"
     CDS             complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="JN334_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HAY7439107.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7766..9046
                     /gene="kbaZ"
                     /locus_tag="JN334_003045"
     CDS             7766..9046
                     /gene="kbaZ"
                     /locus_tag="JN334_003045"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HAY7439108.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9069..9542
                     /gene="agaV"
                     /locus_tag="JN334_003046"
     CDS             9069..9542
                     /gene="agaV"
                     /locus_tag="JN334_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HAY7439109.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9553..10332
                     /locus_tag="JN334_003047"
     CDS             9553..10332
                     /locus_tag="JN334_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HAY7439110.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10322..11131
                     /locus_tag="JN334_003048"
     CDS             10322..11131
                     /locus_tag="JN334_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAY7439111.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11218..11652
                     /locus_tag="JN334_003049"
     CDS             11218..11652
                     /locus_tag="JN334_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY7439112.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            11649..>12062
                     /locus_tag="JN334_003050"
     CDS             11649..>12062
                     /locus_tag="JN334_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY7439113.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         2878 a         3251 c         3078 g         2855 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccaccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacacc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaagg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccaag ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatcccta aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg tcctcatttt
     5041 aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5101 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5161 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5221 aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg
     5281 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5341 ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5401 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5461 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact
     5521 atgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg
     5581 tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggca gcagttgtac
     5641 ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa gtaatggtga
     5701 ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg gatgatgtgc
     5761 aagctattcc agtagaaagc gccagcattg tcagtttgca ggatgaaaag catgtcggtt
     5821 ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc
     5881 aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcat tgcggtggca
     5941 gcgatgcgtt ttctggcgta acggcaaacc cggcggttgg ctatgcgtct gatctactgg
     6001 tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga agtgcgtgac gcgatccatc
     6061 tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt
     6121 ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca
     6181 acaagaaagg cggtctggca aacgtggtag agaaagcgct cggctccatt gctaaatcgg
     6241 gcaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa
     6301 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     6361 tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg
     6421 tcattaaaat ggcaccccgc atcgagctgg cgaaccgctg gtttgattta atggatatta
     6481 atgcgggcac catcgctacc ggcgaagaaa ctattgaaga ggagggctgg aagttgttcc
     6541 actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa tggggactgc
     6601 ataaccagct ggcggtgttt aacccagcac cggtgacctg ataattcaat tcgtattatg
     6661 cccgatagca cacgtttatc gggcatattg agtcacagcg aagacgcgtg atccccgacc
     6721 agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca
     6781 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg
     6841 aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggctt
     6901 aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat cgcatctaca
     6961 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa
     7021 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     7081 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtacttg
     7141 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     7201 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt gttcagtgcg
     7261 cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgcca
     7321 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt gcggatcgtc
     7381 acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc
     7441 agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa
     7501 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct
     7561 attaacgcct ttctattaag caaatgcaag cccaccttgc ccattggcgc aagctactct
     7621 cgtttcagtg actttcatta cgtttctttt gtgaatcaga tcagaaaact attatctttc
     7681 gttttatttt tatctcacca tgacgcagta tcaattgaaa caaaacgaaa gatcaatatc
     7741 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     7801 agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     7861 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     7921 ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     7981 tcagctcgcc gactcgttga atttcccgca ggatgcgctg attctgggtg gtgaccatct
     8041 ggggccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg ccgatgattt
     8101 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     8161 tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     8221 gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg agtatgtcat
     8281 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     8341 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg
     8401 tttgaatgcc atctggccac gcattattgc cctggtggtt caacccggcg tcgaattcga
     8461 tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc agatggtcga
     8521 aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatctct
     8581 gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc tgaccttcgc
     8641 cctgcgtgaa gccctttttt ctctggcggc gattgaagaa gaactggtac cagcgaaagc
     8701 ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca
     8761 aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttaca gctactcaga
     8821 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg
     8881 taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc cgctgcagta
     8941 cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta taaaccatat
     9001 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
     9061 aacatgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg
     9121 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg
     9181 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
     9241 tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga
     9301 aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc
     9361 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca
     9421 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
     9481 tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt
     9541 gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt
     9601 atcgctggcc tagatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc
     9661 ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg cgatacgctg
     9721 gaactagtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc
     9781 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc
     9841 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
     9901 atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
     9961 gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    10021 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    10081 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    10141 ctgaaaatca tgatgaaaaa cgtctacata ccctacttca tcctgggctt tgttgccgca
    10201 acctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    10261 gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    10321 gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac
    10381 tggcgtcaat gaaaacgtgt atgaagatcg gagcattgcc gcggagctga cgaaaaaaga
    10441 tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    10501 gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    10561 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    10621 ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    10681 tattcagagc accaaaattg ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt
    10741 ctggctaacg ctactgccga tttgtggcgg gataggcgca agcctcgcgc tacaaggctc
    10801 tattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt
    10861 tggtctggcg cattatgctt accgcatggg cgtggcggcg attcccctta ttaaagcgaa
    10921 taccaaaaaa gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt
    10981 ggcaacctat gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg cagtagttaa
    11041 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct
    11101 gaccatgttc tggctggtac gccgcggcta gagtccgctg cgcctgattg cggttaccgt
    11161 ggttctaggg atcgtcggta aattctgcca tttcctttaa atacaatgag gtattagatg
    11221 ttaagtatta ttttgacagg gcatggcggg tttgccagtg gcatggaaaa agcaatgaag
    11281 caaatcctcg gcgatcaatc gcagtttatt gccatcgatt ttcccgaaac ctcaagcgcc
    11341 gcgctgctta catcgcagct tgaagaggcc atcgcccaac tggattgtga agacggcatt
    11401 gtttttctga cggatttgct gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg
    11461 caaaaaccgg gctgtgaagt gattaccggc accaatttgc aactgctact ggagatggtg
    11521 ctggagcgcg aagggttaag cggtgaagaa ttccgtgtac aggcgctgga gtgtggacac
    11581 cgagggctga ccagtctggt ggacgagtta ggccgctgcc atgaagaatg tccggtcgag
    11641 gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct gactgaagag ggatggttcg
    11701 atgaccatca gttgcgtgtt gctgacggtg tcatcgcagc aatcgaaccg attccagtgg
    11761 gcgtgactga acgcgatgcg gaacggctct gccccgctta catcgacacc catgtacacg
    11821 gtggtgcggg cgttgatgta atggatgacg cgccggatgt actcgacaag ctggcaatgc
    11881 acaaggcacg cgaaggtgtc ggcagttggt taccgaccac cgtaaccgcg ccgcttaata
    11941 ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca acgcggcgga cctggtgcgc
    12001 aagtgctggg gagttatctc gaaggaccgt acttcacgcc gcagaataaa ggcgcgcatc
    12061 cg
//
LOCUS       DADLTY010000133        11936 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.133, whole genome shotgun sequence.
ACCESSION   DADLTY010000133 DADLTY010000000
VERSION     DADLTY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.133"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="JN334_003051"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="JN334_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HAY7439114.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="JN334_003052"
     CDS             complement(2005..2928)
                     /locus_tag="JN334_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7439115.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3145..4488
                     /locus_tag="JN334_003053"
     CDS             3145..4488
                     /locus_tag="JN334_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY7439116.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4713..6368
                     /locus_tag="JN334_003054"
     CDS             4713..6368
                     /locus_tag="JN334_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HAY7439117.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="JN334_003055"
     CDS             6508..6732
                     /locus_tag="JN334_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HAY7439118.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="JN334_003056"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="JN334_003056"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HAY7439119.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8306)
                     /locus_tag="JN334_003057"
     CDS             complement(7326..8306)
                     /locus_tag="JN334_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439120.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8430..9422
                     /gene="tehA"
                     /locus_tag="JN334_003058"
     CDS             8430..9422
                     /gene="tehA"
                     /locus_tag="JN334_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HAY7439121.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9419..10012
                     /gene="tehB"
                     /locus_tag="JN334_003059"
     CDS             9419..10012
                     /gene="tehB"
                     /locus_tag="JN334_003059"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HAY7439122.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10315..10971
                     /locus_tag="JN334_003060"
     CDS             10315..10971
                     /locus_tag="JN334_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HAY7439123.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="JN334_003061"
                     /pseudo
     CDS             complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="JN334_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11503..11936
                     /locus_tag="JN334_003062"
                     /pseudo
     CDS             11503..11936
                     /locus_tag="JN334_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         3092 a         2790 c         2997 g         3057 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cttcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agtagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagacc
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgcaaaat
     3001 attattaaca attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg cggttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcaga
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cacgtaaact aatgagtcat gaatggtgac actgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata
     8221 atctgccgta ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt
     8281 tcttcactta ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta
     8341 gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac
     8401 tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg
     8461 caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca
     8521 gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca
     8581 tctggggatt attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc
     8641 tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga
     8701 tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt
     8761 ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat
     8821 ctcaccctga agaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta
     8881 tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg
     8941 caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg
     9001 gagaattacc cacggcactg cggacatcac tcggcattca gctcgctcct gcgctggtgg
     9061 cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt
     9121 tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc
     9181 agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg
     9241 gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt
     9301 ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc
     9361 agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat
     9421 gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga
     9481 agtactggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa
     9541 tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa
     9601 tgccatgagt atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca
     9661 cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc
     9721 gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca
     9781 acgttgcact aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta
     9841 tccatgtacc gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga
     9901 aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa
     9961 cggtaatcgt attaaactgc gtttcgccac gatgctggca cgtaaaaaat gacccggtaa
    10021 gcacaaaacg cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag
    10081 catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt
    10141 aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt
    10201 cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag
    10261 cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt
    10321 accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc
    10381 tctaaaatca cccagccaga taaatataac aattactctg atttaaaaga aacaacctca
    10441 gcaacaggta aacctgtttt acgttgggta gacccgagtt ttgatcaaag caaatatgac
    10501 agcatcgtct ggaacccaat cacttattat ccggtaccaa aaccttctac ccaggtaggg
    10561 cagaaagttc tggataaaat tttgaactat accaacaccg aaatgaaaga agcgatagcg
    10621 cagcgtaaac cagtggttac caccgctggg ccgcgtagtc tgattttccg tggggccatt
    10681 accggtgtag ataccagcaa agaagggctg caattctatg aagtggttcc tgttgcatta
    10741 gtggttgcgg ggacgcaaat ggctacaggc caccgtacca tggatactcg cctctatttt
    10801 gaaggtgagc tgattgatgc agcgactaat aaaccggtta tcaaagtcgt tcgtcagggc
    10861 gaaggtaaag acctgaataa cgaaagtacg ccaatggctt tcgaaaatat taaacaagtt
    10921 attgatgaca tggcgaccga tgccaccatg tttgacgtta acaaaaagta gttccagacg
    10981 cgccatcgtt agatggcgct ttttatccgg tgcgccgtaa aaccccatcc ttcagggcgg
    11041 ggatataagg cgcggttttc cacctgacca ggtgtttgct gttgttcaat gtattggcgg
    11101 atgacggata ttggcgcacc tccgcagcta ctggcaaaat agccaggact ccacaaaacg
    11161 cctttgtagt aatacctgac tgcaatatct ggtcgatctc gtcgcagtaa cctacccgat
    11221 acgcctttga ggctgtttac cagactggat atcgccagtt tgggaggtta gttaattaac
    11281 aaatggacgt gatctggttc gccatccatt tcaaccagtt cagcttcaaa ataagcacat
    11341 acatttgaaa agtaagtgcg tagtttttct gtcgcgtcat ggtcaaaaat ctggcgtcgg
    11401 tatctggtga caaagactag gtgaacatgc atcaggaaaa cacaatgcct tccacgccgg
    11461 atatcggttt cttttttcac agaccaaagt atcatttgac ctgtgaaacg attacaggca
    11521 tttaaattcc agttaagacc aggtggtcaa caggagtgtg aaatgaggcg cttcgcaggc
    11581 gcatgtcgtt tcgttttcaa tcgtgctctg gcacgtcaga atgagaatca tgaggccggt
    11641 aataaataca tcccttacgg gaaaatggct tcctggctgg ttgagtggaa aaatgccact
    11701 gaaacgcaat ggcttaaaga ttctccctca cagccattgc aacagtcact gaaagacctt
    11761 gagcgggctt acaaaaactt cttccggaag cgggctgctt ttccccgatt caaaaagcgg
    11821 tgacagaatg atgcattccg ctacccgcag ggcgttaagc tcgatcagga aaacagccgt
    11881 atttttctgc cgaaactggg ctggatgcgc taccggaata gccgtcaggt cacggg
//
LOCUS       DADLTY010000134        11924 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.134, whole genome shotgun sequence.
ACCESSION   DADLTY010000134 DADLTY010000000
VERSION     DADLTY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.134"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..2341)
                     /locus_tag="JN334_003063"
     CDS             complement(<1..2341)
                     /locus_tag="JN334_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HAY7439124.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2275..2898)
                     /locus_tag="JN334_003064"
     CDS             complement(2275..2898)
                     /locus_tag="JN334_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HAY7439125.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2895..4583)
                     /locus_tag="JN334_003065"
                     /pseudo
     CDS             complement(2895..4583)
                     /locus_tag="JN334_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="JN334_003066"
     CDS             complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="JN334_003066"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HAY7439126.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7506..8228
                     /locus_tag="JN334_003067"
     CDS             7506..8228
                     /locus_tag="JN334_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HAY7439127.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8356..10287)
                     /gene="yfaL"
                     /locus_tag="JN334_003068"
     CDS             complement(8356..10287)
                     /gene="yfaL"
                     /locus_tag="JN334_003068"
                     /inference="COORDINATES: protein motif:HMM:NF007285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HAY7439128.1"
                     /translation="MEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMAD
                     SSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGDG
                     ATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDG
                     VTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTL
                     LFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQN
                     NAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDPT
                     PDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHYT
                     AAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADNQ
                     NHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLEA
                     GYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTAV
                     GVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAWQ
                     KGSDDFAQTAGFLSMTVKW"
     gene            complement(10300..11895)
                     /locus_tag="JN334_003069"
     CDS             complement(10300..11895)
                     /locus_tag="JN334_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HAY7439129.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2757 a         3307 c         2945 g         2915 t
ORIGIN      
        1 attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc acccggatat
       61 caagctgagg aacggcaact ttgatcccgg cgttctgaaa actgtactga ccattacggg
      121 tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactggt acccgggctt
      181 catacagggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc gaaagcagcg
      241 actgctgttc cacgcgatcg cgttccagcg tcaataacgc ttcatcaatt ggctccggga
      301 aagtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatcc gccacgatat
      361 ctaccgtacc agtatgcgcc gtgctgccct taccgctgac ggcatgactt aacccagcga
      421 gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta gcgaaattga
      481 cggtaaagga tttgccgcct gacggtagct ctccgctatg gctcgtgcgg tcttcgagac
      541 gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca
      601 caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc
      661 cgcgttcaat gaggatctct ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg
      721 agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaac gccacatgac
      781 cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac ggggaaacgt ccggcataac
      841 gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc acgcgggcat
      901 tttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc gcttcgccag
      961 ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg atgtagtttg
     1021 ccacgcgaaa actgctgcta tagacctgat tgcggtaagc aagacgtaac tcataacctc
     1081 cggcaacggc attttctggc aggcggaaac ttccctgccc gccattgcgc gcatccagcg
     1141 tgacatcgac ggtttgcaac agactgccgt tagcgtccag caccgaaagc ttcgccgggg
     1201 cgctgacgat gggggatgaa tgcaacggat cgcggaactc gcggcccatc actttaacat
     1261 cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa aatatacaag cgggtgttgt
     1321 agatttcgct ttcgtagaag aagttctcgg agacaaaaac gccgccttca gcatccttac
     1381 ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaagtaccg ctgtcatcgg
     1441 tcaccccgcg ggtcatcacg ccaagacctt cagtccacaa gatctctgag ccgggcttcg
     1501 cttcaccctg ttttttaccc gcggtccata ccagaagctc gttacctgac actttgctaa
     1561 gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct accatcgcct
     1621 cgacgaggta cagccccggc tcttgttggc cgagaggaat ataaatatta cccggctgcg
     1681 gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc tcgaccggtt
     1741 tagcctgcca tagtggataa cggaactgtt ccaccaaggg atattttttc agcggggaaa
     1801 actggttgtt ctgtacataa cgggaaggtt taataatggc attgccgaga tgtaattcgg
     1861 gtaatgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc atcacgcggc
     1921 gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagc ccgtcgccca
     1981 gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg
     2041 ggtcaggaat acgatatagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc
     2101 gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc
     2161 tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc atagttactg gaaggaagcg
     2221 aatcatcagc tttagcaagc cctgttccca caagagacag ggacaagcac gctgctatcg
     2281 cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca
     2341 tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt
     2401 tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca ttaagtgctg
     2461 ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta tgtcttggcc
     2521 aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggggc cgtttttccc
     2581 gctcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg tcatctctgg
     2641 cggcaaatac tggctagata aaccgttact ttttagccat ttactgtcgt gaactttcag
     2701 cgtctcgttc gccgcaaatc gcaccaggcc cgcacaatcc tgctgatacc agcgtggact
     2761 tggcccctgg cggagctgtt cttgtgcaat gcgtacaaac caggcgcgaa acagcctgga
     2821 ttgttcgaca ttcagcattt cgctatgggc aacaacacaa cacagccagc aaatcagcgc
     2881 cagcagcccg tgtctcatag cggctgccag gttatgggta gccactgcca ggcgacaccg
     2941 ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc gtccagcttc
     3001 ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt cggcagactg
     3061 gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata gagcggaacg
     3121 atgccatcag tgggtattac atccaccatt gccgggcggg ttttattcag tgtttgcagg
     3181 gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg catcgccagc
     3241 gacacacgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg cgctttcgga
     3301 tactgaccgt atcgggaact cacttcgcga caccagattt gcgcttcgcc ctgctgagtc
     3361 tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat attttgcgta
     3421 aacagtttcc ctggcaattg cgcctgttcg gcagtgccat caaactgacc gacaaacagc
     3481 ggggtttgca attttgagtc ttcataccag cacagccccg cggcaccgtc taacgcccca
     3541 ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat accgtgtgaa
     3601 tacggcaccg ccacacagaa gctggctccg gcaggcatac tgttccagac cggcgtaaaa
     3661 tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaaact gtgccagccg
     3721 tcgttaccca tttcgaagcg tacgccagca aaagaaggca ttaaccgctg gtagccaaaa
     3781 cccagcaacc tggcgctgac cacgatgcgc aggcgtactg gcgttttttc ggctgtacgc
     3841 tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc acttgcgatc
     3901 gccgtggctt cggtatcctg ctgatcatct ttaaacaaca tatccgtact ggaaaacacc
     3961 agcatcttgt cctgatatgt cgcgaacatc agggcgttat tgccgttata gcgcaactga
     4021 taaacaggaa tagtttcact atttatcttg atgctactga tttccgtttt gcttaactag
     4081 ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt tgcttaaacc actgcgctgg
     4141 atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc cgcctgcgcg
     4201 ggctgatcta agagtgacga aaagagctta tctttcagcg tgagatcgtg ttcatagaca
     4261 atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata atagaaaacg
     4321 aaatcttcgc tcagaacatc gtggagaaac ggaatggtga ggagatcttt gggaagctgg
     4381 ctcagagagt cgctgtctag aaagaggtcc ggctcattga gatcgatttg cagattgttg
     4441 tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg caacgcccag
     4501 acgccagcgg aaagcagtgc tattgcgcca aaacctacaa gaccatagaa ccgccagcct
     4561 ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc attttttacg
     4621 ggcacagcca aactttaccg cgccctaata cgacaaaagc ccagactttg cagcctggac
     4681 ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa attattcttc
     4741 ttctggctcg tcgtcaacgt ccacttccgg agcaatttca tcatcccctt ccgcggcact
     4801 gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgct gcagacccac
     4861 tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac ggcccacgat
     4921 gctgatttct gaaacgcgag tacgtaccag cgtaccggca tcggtgatca tcatgatctg
     4981 gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg taaccttgat
     5041 ggagataacc cctttcgtcg cacgcgactt ggttgggtat tccgccactg cagtacgttt
     5101 accgtaaccg ttttgcgttg cggtgaggat tgcgccatcg ccacgaggca cgatcagaga
     5161 gacgacttta tcgccttcac ctaagcgaat accgcgcaca ccagtggtat tgcagcccat
     5221 cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga acagcattac
     5281 ttcatcttcg ccactggtca gatcaacgcc gatcagctca tcgccgtcaa ccagtttgat
     5341 cgccacttta ccggcggtac gcagacggtt gaattcggtg aggacggttt tcttcacagt
     5401 accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca ccggcaggat
     5461 cgcggtgata cgttcgtcct gttccagcgg tagcaggttg acgatcggac gaccgcgcgc
     5521 gccacgagtg gcttccggca actggtaaac tttcatcgaa tagacgcgac cacggctgga
     5581 gaagcacaga atatggtcgt gcgtgttcgc caccagcagt cggtcgataa agtcttcttc
     5641 tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcgt attcagaaag
     5701 cggctgatat ttaacgtagc cctggtgaga gagcgtcacg accacatctt cctgggtgat
     5761 cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt tcagtacgac gtttgtcacc
     5821 gaactgttca cgaaccagct ccagctcttc gcggatcact tccatcagac gatcggcgct
     5881 accaagaata cgcaacagtt ccgcgatctg atccagcagc tctttgtatt cgtcgagcag
     5941 tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg cctgagcttg
     6001 ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tctggctcca gccattccgg
     6061 acgcgcagca tcgtcgccag cacgttccaa catggcggca acgttgccca gctgccacgg
     6121 attagcaacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat
     6181 gatcgggtca atgttcgcca gcgccacggc taatgcttca aggatatgag cacgatcgcg
     6241 agctttacgc agttcgaaaa tagtacgacg ggtcaccact tcacggcggt gacgaacaaa
     6301 cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca atgccaccat
     6361 gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga gcacaacttc
     6421 accgaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt cagactcgtc
     6481 acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa tcttctcgat
     6541 caggcgcgct ttgtttacct gatacggaat ttcgtggacg ataatggttt cacgtccggt
     6601 tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc gaccggtacg
     6661 gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga agtccggccc
     6721 cgggatgtgt tccatcagcc cttcaatgct gatgtcttca tcatcgatat acgccagaca
     6781 accgttgatg acttccgtca ggttgtgcgg cgggatgttg gttgccatac ctacggcgat
     6841 accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt cgggaatttt
     6901 ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat cggccatcag
     6961 ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg cagagtcgcc
     7021 gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg agaatggctg
     7081 cgccatacgg acgatcgtgt cataaaccgc cgagtcacca tggggatggt atttaccgat
     7141 tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat tgcctagtac
     7201 gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga catctggcag
     7261 cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttta gctcttcctc
     7321 aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat ccctctactg
     7381 tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg taaacctata
     7441 cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac ggagtagaag
     7501 tgccaatgaa tgccgaaaaa tcgccggaaa accataacgt agaccacgaa gagatcgcta
     7561 aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa ccgttgcacc
     7621 gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta tttggcaaaa
     7681 aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg cgcgaaggcg
     7741 cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag ctgcacgcgc
     7801 tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac
     7861 atgccgggca gtatgatgtg gtgacctgca tggagatgct ggagcacgta cccgatccgc
     7921 agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg cggcgatgtc tttttctcga
     7981 cgcttaaccg caacggcaag tcatggctga tggcggtggt tggcgcggaa tatattttgc
     8041 gcatggtgcc caaaggtacg catgatgtga agaagtttat taaaccggca gagctgcttg
     8101 gctgggtgga tcagaccagt ttaaaagagc gacatataac cgggctacat tacaacccga
     8161 tcactaattc ctttaagctc ggccccggcg tggatgtgaa ctatatgctg cacacgcaga
     8221 ataagtgagg ttgatgtttg gccgcgccaa tgcctgatgc gacgcttgca gcgtcttatc
     8281 aggcctacaa atgctctccg taggccagat aaggcgttta cgccgcatcc ggcaattgtg
     8341 atggtcagac agcgattacc atttcaccgt catcgacaaa aaccctgccg tctgggcaaa
     8401 atcatcactc cctttctgcc acgccacgct gccgcgcagg gacactcgct gactgatatt
     8461 gcccgtgact cccactttta tttcaccccg tagcttcacc gcatcgtcac tgatagtgct
     8521 gccatcttct tcaatttccg tcgcatgggg atcgtgatag taattcagat ctaatgttgg
     8581 tgtgacacca accgcggtac gccattcgct gtgtaaaccc agccgcgtct gaatatcatc
     8641 cccctgcgat tgtgacacgc gcgcatggtt agcggcggta aaatcatcct gctgcacgcc
     8701 ctgataaatc acctgcgcct gcggttcaat caccacacca cgccccggta accactgata
     8761 cccagcttcc agcgaggcga taatccccga cgaatggtaa cgatccacgc catcttcatg
     8821 ttcggaaaca tcattgttaa accacgcgta ttgcagccag ctatccagcc aggccccctg
     8881 cttctgatta ccgtgctgat accagcttga ggtcagccca acggcataac cgtggttctg
     8941 gttatcggcg cgagttccgg tcatattcga gcggctgtcg ccctgattat cactgtagcc
     9001 accaaccgcc ccaagcatcc actcgccatc cgtgccccag cgcccgctaa acaagccgcc
     9061 gctaagctgc accgtagaag tgtcttcctg ctgagccagt tgccctgccg ccgtgtaatg
     9121 ataacgtccg ccgataacac gtaaattcag cgtctgacca tcgccaccag cgtgatcgcg
     9181 tcgctccatc ataaacgcct gatttgccgc ccgcaggtta ttgagataac cgccaacttt
     9241 ggcattcagc accggctggt aagcaggcgt aggggccggg tcgggggttg ggtcaggatc
     9301 cggcgtggga tcaggatcgg gagtcgggtc tggctcaggt ggcgatggcg gcgttacttc
     9361 ttgcgatcgc agataccagt cgttgttatc ttccactagc gtgtagtcat acgctcccat
     9421 gttgacgtag ccactgccag ccagactgaa ctgtgcattg ttttgaaact gcgtgggatc
     9481 agctgcgaaa tcaaccactt taatgcctgt cgatgtcggc tcaccaatcc ctgtaatgga
     9541 gttaaccacc acagttgtgt tgccagcagt attaccgttc atcaccaatt gatcgcttac
     9601 cgagtcatcg ccgtttaatt cgctgtcgaa tagtagcgta ccgccgccgg tataatcacc
     9661 gtttaccgtc agcgtatcgc cagcgacgcc attttgcagg ctaaccgcac cggacgtatt
     9721 ggtcagatca ccattaaccg tcacgtcagc aagataggtc aggctgtcag tttcaaggtt
     9781 ggtattaagc tcgcctgtca acgtcacgcc atcggtagca tttaccagcg tcccatcgcc
     9841 gttaaatagt gaggcattaa gggcgacgga agtatcctgc ccggtcagac gcaaaaccgt
     9901 accatcgctg atgtcgatag tgccgctgga agtagcatca attgactgaa tatcctgatc
     9961 cgcgccagta acgaacgttg ccccatcacc aacccacagt gacgaagcat aaggaaagat
    10021 atccgcaaca tcgcctttca gggtgccttc ttcgacacgt accgccgact gcgttgtacc
    10081 gctggcggtc agctccagcg tacccgcccc cgttttagta aatgtgctac cctcatcctg
    10141 atgctgcccg ctgctgtcag ccatcagtgc gccccactgc gtgtctaccc ccgcatcaac
    10201 tgccacttca ccgtcggcgc gcatttcaat atcacgccca tgctcagtcg tcgtagagcg
    10261 agtatcgaca ttggcagtaa tctccatcac gctttcttgt tacttatcgg taaagataac
    10321 ctggcggtta tagtgggtat ctccaagctg cgagttgtcg ctcaccatca gcgtaccgga
    10381 ggcgatttgt gttgtgccca aataactatt attgtttgcc agacttacct gcccgtcgct
    10441 cgcatcgacc accacgtaga cgccatcttt cccgtcgccg ccgatatgca aattgtcgcc
    10501 acctgcaaga tctaccacat cctgactgcc gataaccgtt ccgctgctgc cactgacttc
    10561 aaggccatca ttgtatgatg tgccgctctg ccaagtggag aaatcagaga gatcgagcac
    10621 accgccgttt aacacgatcg actgcggatc gtcctggaga gcggtaagat ctgccgcgtc
    10681 gccttccagc gaaagcaccg caccatcatc aacgactata tcgccggtta gcgccatcga
    10741 ctgcgcccct gccagcacgt agctgccgtt ttgcgcaatg gtgagctgac cagcaccttc
    10801 gatgatgcca gcaaaactgc cctgattaac agtaacgttg ccaccagcat cgatatttaa
    10861 agtgccattc tgaaagcccg tcaatgcgtg cacaaaagtt tgttgggtcg agccaacgtt
    10921 tagctcagcc tgattctgat actgatcaat actccctatc gtcagaccgt agcagtcttg
    10981 cggatcgtcc tggcaatgcg tatcgccgac attcatcagg gagttgctgc gacccagggt
    11041 aacttcaccg ttttcaatct gcatctcacc agtaaagtca ttgttatctg cattaagtac
    11101 cagatcgccg gaacctgttt tggtgattaa cccggtacca gcaatagagt caacagctcc
    11161 gtcattctct gtattgccga taaccagcgt ttttccgtcg gcaatatcaa aggtaacttc
    11221 acttaagccg agatacataa agccacccgc cgcagtggag ggaccatctc catagcctgc
    11281 tgcgctattg ttctcatcga ctaacacgcc gccgttctgg ctgtagctgt catcaacaga
    11341 aatatcaata agatagggag ccgtcgcgct attggtatat atcgccccgc cataaccttc
    11401 ggctgtgtta tttgtaaagg cggtgttatt tattatcgta taacctgaag gatgcttgct
    11461 gtcgctatta ttatcggtaa catcgattgc cccgccatcg ccatcactgt aacttgttga
    11521 tgtatatgcc tggttattgt taaaaacatc atcacttaaa tagacgtcat tattaatggt
    11581 ataaattgcg ccacctttgc catcattagc gatattattg atggcgttag tgacacgtaa
    11641 atcgacggca ccagtatcat tagtaccaga agaatagatt gcgccaccat agccgcctgc
    11701 gacgttaccg gaaaaaataa catcagtaag attcagcgtt gagttttctt tagcaaatat
    11761 ggccccgccg ttattatatt ctcctgtaac ggtgttattg gcaaacagag tcattccagt
    11821 ttcattttct ggtaatagtg aaaattccgc tccttgttgc aaaaatacgg ccccaccgct
    11881 ggcattattg gtcatatccg aaaaaaccag ccattgccca tcgg
//
LOCUS       DADLTY010000135        11682 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.135, whole genome shotgun sequence.
ACCESSION   DADLTY010000135 DADLTY010000000
VERSION     DADLTY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.135"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..346
                     /locus_tag="JN334_003070"
     CDS             <1..346
                     /locus_tag="JN334_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HAY7439130.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
     gene            complement(463..738)
                     /gene="yjfN"
                     /locus_tag="JN334_003071"
     CDS             complement(463..738)
                     /gene="yjfN"
                     /locus_tag="JN334_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HAY7439131.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="JN334_003072"
     CDS             complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="JN334_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HAY7439132.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1398..2147
                     /gene="yjfP"
                     /locus_tag="JN334_003073"
     CDS             1398..2147
                     /gene="yjfP"
                     /locus_tag="JN334_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7439133.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="JN334_003074"
     CDS             complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="JN334_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HAY7439134.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="JN334_003075"
     CDS             complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="JN334_003075"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAY7439135.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4426..5823
                     /gene="ulaA"
                     /locus_tag="JN334_003076"
     CDS             4426..5823
                     /gene="ulaA"
                     /locus_tag="JN334_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAY7439136.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5839..6144
                     /gene="ulaB"
                     /locus_tag="JN334_003077"
     CDS             5839..6144
                     /gene="ulaB"
                     /locus_tag="JN334_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAY7439137.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6154..6618
                     /gene="ulaC"
                     /locus_tag="JN334_003078"
     CDS             6154..6618
                     /gene="ulaC"
                     /locus_tag="JN334_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HAY7439138.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6632..7282
                     /gene="ulaD"
                     /locus_tag="JN334_003079"
     CDS             6632..7282
                     /gene="ulaD"
                     /locus_tag="JN334_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAY7439139.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7292..8146
                     /locus_tag="JN334_003080"
     CDS             7292..8146
                     /locus_tag="JN334_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HAY7439140.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8146..8832
                     /locus_tag="JN334_003081"
     CDS             8146..8832
                     /locus_tag="JN334_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HAY7439141.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8961..9236)
                     /locus_tag="JN334_003082"
     CDS             complement(8961..9236)
                     /locus_tag="JN334_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7439142.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9563..9958
                     /gene="rpsF"
                     /locus_tag="JN334_003083"
     CDS             9563..9958
                     /gene="rpsF"
                     /locus_tag="JN334_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAY7439143.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9965..10279
                     /gene="priB"
                     /locus_tag="JN334_003084"
     CDS             9965..10279
                     /gene="priB"
                     /locus_tag="JN334_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HAY7439144.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10284..10511
                     /gene="rpsR"
                     /locus_tag="JN334_003085"
     CDS             10284..10511
                     /gene="rpsR"
                     /locus_tag="JN334_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAY7439145.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10553..11002
                     /gene="rplI"
                     /locus_tag="JN334_003086"
     CDS             10553..11002
                     /gene="rplI"
                     /locus_tag="JN334_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAY7439146.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11073..11630)
                     /locus_tag="JN334_003087"
     CDS             complement(11073..11630)
                     /locus_tag="JN334_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY7439147.1"
                     /translation="MNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDVE
                     SFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCCSSELPTIDLYIDQLH
                     TVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRSD
                     RMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         2767 a         2872 c         3087 g         2956 t
ORIGIN      
        1 cggcaatatt atgtgcctgg atgtgttgcg cgttctcaat aagcaagcgg gcgtatacga
       61 cttattgtcg gaagcatttg tggaagtgaa agggcaggat cgctattttg atcgcgcggt
      121 tcgtcgttta cagcagcagc tgcgtaagcc agctgaagaa ctggggcgag agattactca
      181 tcagctattc ctgctgggct gcggtgcgca aatgttgaaa tatgcttctc cgccaatggc
      241 gcaggcgtgg tgtcaggtga tgttagatac gcgcggcggc gtacggttgt cagagcagat
      301 ccagaatgat ttattgctgc gggcgacggg gggagtgtgt gtgtaagcgt atacgactga
      361 tgcgacgctg gtttcgatta actaaatgaa atatgtgaaa attgtaggcc ggacaaggcg
      421 ctcgcgccgc atccggcatt gttcatttgc tgtagatgac actcatgcat acagtatcgc
      481 ctgtacgcgc cagttatcag gctgctggtc ttcgtacatg gtaataatgc ggtaccatga
      541 cgcaccttgt tcatcggcct ttaacgccac gatacgttcc acgtcctgcg gatcccctga
      601 aatattgcta actgagatct gacctatttc attcaggccc gtcacgcagt cagcactggc
      661 gaattctgca gactgtgccg tggcgctcgc aggataagtc actaattgca gcgagggtga
      721 ggcaagtaat tgtttcatcg tcagctccat ttttcgtttc cccacatgtt gcagggcatt
      781 ccatcgcggt aataaacgcc caagcttcca gccggtaatt tattgtgcac aggtaaacgc
      841 atagggacgc aaagcaatgt aaagctgact atgtaagttg tctgatttat ttacggtaca
      901 aaatggcttg tgaataccac tgtcctgtca cgatggtttc gtccaccatg acgacaacgt
      961 aataatcagc ttttgcagcg acagcttttg ctctgatttc tgctaatgcg tcatccggag
     1021 aaccccgaac catcgtgctt acgctaccta ttcgctgtaa cccttgcgtc tggtcgcggc
     1081 gaatctcttg cggatggtcc gttactggcg gtgctggctg tggcgtacct tgcagtgcgc
     1141 tacaggcact taacatcaac accaataata aactggcaaa ccggtaaata acgctattac
     1201 gtttcctgct aaccatagtg tagtgcctta ttaatttaac tttgggggaa tgttcccgaa
     1261 ttgttacctg gtacgcgatt ttcgaatgaa aacgtgacga aagcgtaacc cacatctcaa
     1321 cattatgaac taataccgcc agcagaaact tgtgctaagg atttcttcat gtcataccag
     1381 acaaaaagga gagacagatg attgaaatag aatcacgcga gctggcagat attcccgttc
     1441 ttcatgctta tcctgtcggg caaaaagata ccccgttacc gtgcgtaatt ttttatcacg
     1501 gctttacttc atccagcctg gtgtatagct attttgccgt tgcgctggcg caggctggtt
     1561 tgcgggtgat catgccggat gcgcccgatc acggtagccg ttttagtggc gacgaagcgc
     1621 ggcggttaaa tcaattctgg caaatcttgc tacaaagtat gcaggaattc actactttac
     1681 gtgcggcaat agccgaagaa aactggctgc ttgatgaccg tctggcagtc ggtggcgcgt
     1741 cgatgggcgc gatgacggca ctgggaatta ccgctcgcca ccccacggtg aaatgtaccg
     1801 ccagcatgat gggatcgggc tattttacat cgctcgcccg ttcactgttt ccaccgctga
     1861 tacctgaaac ggcagcacag cagaatgaat tcaataaaat tgtcgcgccg ctggcagagt
     1921 gggaagcgac aaaccacctg gaacaacttg gggacagacc tctactgctg tggcatggcc
     1981 tcgacgatga cgttgtgcct gccgacgaat cactacgttt gcagcaggcc ttaagcgaga
     2041 cgggacggga taaactgcta acctgttcat ggcagccagg cgtgcgtcac cgcattacgc
     2101 ctgaggcgtt agatgctgcc gtgacatttt tccgccagca tctttaaaca cgcagaatgc
     2161 tgaccccttg cgcttccagt tgttgcagga tttccgggtt agcatttttg ccggtgatca
     2221 gcatatcgat ttgatcggca cggctaaaaa gcattcccgc gcgttcgcca atcttactgc
     2281 tatcaaccag taccaccagt ttccctacca cgctcagcat cttctgctct gccattgctg
     2341 tcagcatatc ggttttatac aacccttctg cggtcagccc ttttccgctg gtaaacatcc
     2401 agtgcccggc atagagactg ttttcgctgc cctgcgggct taaagtgatg gactgacttt
     2461 tgttgtactg tccacccata atgatcacgc tgtcatgttc ttgatcgatc aggtaatttg
     2521 ccagcggtag ataattagtg atgatttgca ctggcttgcc acacatttcc cgcccaagca
     2581 gaaacgcggt ggagccgcag ttgatgacta cgctttcgcc cggattaacc agctgcgagg
     2641 ccgctttagc aatacgtact ttttcatcgt gattctgcgc ctgatgcaga ttcatcggcg
     2701 tccagcgcgg gcgctgttgg gtaatagctt ctgcgccatt gcgcaccttt ttcagtttgc
     2761 cgctttcgcc aagtttattg atatcgcgtc gcgcagtggc aggcgaaatt cccagacgct
     2821 caacgacttt ctcaacggtc acaaagccca attgtgcgag catttccagg aggatttgat
     2881 gtctttgtgc ttcagtcatg agctattccg ataaaatttg atttttttag atgatatttg
     2941 aaatagccag gaaatactac gccggatagc gcgaacttct ccacgctatc cggtataaac
     3001 gacagattac aggaatgact tgaacggcag atccggttca atagtgaagc aatcatcgaa
     3061 accgcgcgga tagtggtact cgaagttgtc tttatccagc ggccaggtaa atttgccgcc
     3121 aacctgccag ataaacggct tgaagccata cttcagacga tcttttttga tctcccacag
     3181 cacgcggatc tcttgcggat cggcctggaa gtttgaccag atgtcgtggt ggaacgggat
     3241 aaccactttc gcattcaggg cttcacccat acgcagcata tcggcgctgg tcattttgtc
     3301 ggtaataccg cgcgggttct caccatacga tcctaacgcc acatcgatct gatgttcgtt
     3361 accgtgcttc gcataatagt tagagtagtg ggaatcgcca ctgtgatata gggaaccgcc
     3421 aggggttttg aacaggtagt ttactgcgcg atcgtccatg ccatctggca gtacgccagc
     3481 cgctttttga tcggcaggca gggtgatcag tgcagtacgg tcgaaagcgt caagcgcatg
     3541 aatttcaatg tctttcactt ttacaacatc gcccggtttg accacgatgc aacgctcttt
     3601 cggtacgccc cagccaatcc acaaatcaac gcaggttttc ggtccaatga atggtacgtc
     3661 atcagcacag ttctgcatca cggcagcagc gacgttaacg tcgatatgat cgttgtgatc
     3721 gtgagtcgcc agtaccgcgt cgatctggcg aatcgcaaac ggatcaagaa caaacggggt
     3781 ggtgcgtagg tttggctgca gttttttcac gccagccatg cgctgcatct ggtgaccctg
     3841 tttcattaac gggttaccgt gactttgttt gccagtgccg caccagaaat caacgcaaac
     3901 gttggtgcca ccttctgatt tcaaccagat cccggtgcag cctagccacc acatcgcaaa
     3961 tgtgccaggg gcgacctgct cttgttcaat ttcttcattc agccagctac cccactccgg
     4021 gaaagtgctc aggatccagg attctcgggt gatacttttc actttactca tcgccattta
     4081 ccttcatgat agttcaattc gaatcaacat gtgattggtt ttgattaatc ctgacactat
     4141 tccttcagga aggtaatgac cattttttga ctttttgcca gggaagttgt tgttgatttt
     4201 gagtggggaa agatttaatg gaatgtgtag ttaaattaac taaatgaatt taaataaata
     4261 attgtttcgt tgtgtgaatc ccactctatc catgtggaat tatttgcggg tcgcgtcaca
     4321 tttaatcata aataatcttg ttgtgattac ttttgaaaat tagagtgagt gcacaacatt
     4381 ccgggtgtgc ggaatacccg gttacctctt cttcaggaga tcgttatgga gatcctctac
     4441 aacatcttta ccgtgttttt taaccaggtc atgaccaatg ccccgttgtt gctgggtatt
     4501 gtgacctgtc tgggctacat cctactgcgc aaaagtgtca gcgttattat taaaggcacg
     4561 attaaaacca taattggttt catgttgttg caggcagggt ccggcatcct caccagcacc
     4621 ttcaaaccgg tggtggcgaa aatgtccgaa gtctacggca ttaacggcgc aatttccgat
     4681 acttacgcgt cgatgatggc aaccatcgac cgcatgggcg atgcctatag ctgggtaggt
     4741 tacgcggtgt tgttagcgct ggcgctgaac atctgttacg tgctgctgcg tcgcattacc
     4801 ggtattcgta ccatcatgct gactggtcat atcatgttcc agcaggcggg gctgattgcc
     4861 gttacgttgt ttattttcgg ctactccatg tggacaacca ttatctgcac ggcgattctg
     4921 gtttcgctct actggggcat tacctccaac atgatgtaca agccgactca ggaagtgacg
     4981 gacggctgcg gtttctccat cggtcaccag caacagtttg catcattaat tgcctataaa
     5041 gttgcgccgt tcctcggcaa aaaagaggaa agcgttgaag acctcaaact gccaggctgg
     5101 ctgaatattt tccacgacaa catcgtctcc acggcgattg tcatgaccat cttctttggt
     5161 gccattctgc tctccttcgg tatcgacacc gtgcaggcga tggcaggcaa agtgcactgg
     5221 acggtctata tcctgcaaac cggtttttcc ttcgcggtgg cgatcttcat catcactcag
     5281 ggtgtgcgca tgtttgtggc ggaactctct gaagcgttta acggtatctc ccagcgcctg
     5341 atccctggtg cggttctggc gattgactgt gcggctattt atagcttcgc tccgaacgcc
     5401 gtggtctggg gctttatgtg gggcaccatc ggtcagctga ttgcggttgg catcctggtc
     5461 gcctgcggtt cctcgatcct gattatccca ggctttatcc cgatgttctt ctccaacgcc
     5521 accatcggcg tgttcgctaa ccacttcggc ggctggcgtg cggcgctgaa gatttgtctg
     5581 gtgatgggga tgatcgaaat ctttggttgc gtctgggtgg tgaaacttac cggtatgagt
     5641 gcctggatgg ggatggcgga ctggtcgatt ctggcaccgc cgatgatgca gggcttcttc
     5701 tccatcggta tcgcctttat ggccgtcatc attgtaattg cactggctta tatgttcttc
     5761 gctggccgcg cactgcgcgc agaagaagat gcagaaaaac aattggcaga acagtctgct
     5821 taataaggag ttttgattat gaccgtacgt attctggctg tgtgtggcaa cggacaaggc
     5881 agttccatga tcatgaagat gaaagtggac cagtttttaa cccaatcaaa cattgaccac
     5941 acggtaaata gctgcgcggt tggcgaatac aaaagcgagt tgagcggcgc ggatatcatc
     6001 atcgcttcta cgcacattgc gggcgaaatc accgtgaccg gcaacaaata cgtggttggc
     6061 gtgcgcaaca tgctctctcc tgccgacttt ggcccgaaac tgctggaagt gatcaaagcg
     6121 catttcccgc aggatgtgaa gtaaggacgc gccatgaaat tacatgattc gctggcggag
     6181 aataaatcca tccgcctgca ggctgaagct gagacatggc aggacgcagt aaaaatcggc
     6241 gttgacctgc tggtggcggc ggatgttgtc gagccgcgtt actaccaggc gattctggat
     6301 gcggttgaac agcacggtcc gtactttgtg ctcgcaccag gcctggcaat gccgcacggt
     6361 cgcccggaag agggcgttaa gaaaaccggt tttgcgctgg tgacgctgaa aaagccgctg
     6421 gagttcaacc acgaagataa cgacccggtg gatatcctca tcaccatggc ggcggtcgat
     6481 gccaacacgc accaggaagt gggcatcatg cagatcgtca acctgtttga agatgaagaa
     6541 aatttcgacc gtttacgtgc ctgccgtacc gagcaggaag tactggatct cattgaccgc
     6601 accaacgcgg cagcttaaga aggaattgaa gatgtcatta ccgatgttgc aagtcgcgct
     6661 ggacaaccag actatggata gcgcctacga aaccactcgc ctgattgccg aagaagtcga
     6721 cattatcgaa gtgggtacca ttctgtgcgt gggcgaaggc gtgcgtgcgg ttcgcgatct
     6781 gaaagcgctc tacccgcaca aaatcgtgct ggcagacgcc aaaattgccg atgcgggcaa
     6841 aattctttct cgtatgtgct tcgaagccaa cgctgactgg gtgaccgtga tttgttgtgc
     6901 ggatatcaac accgccaaag gtgcgctgga cgtagcaaaa gagtttaacg gcgacgtgca
     6961 gatcgaactg accggttact ggacctggga acaggcgcaa cagtggcgcg acgcaggtat
     7021 tcagcaggtg gtttatcacc gcagccgtga cgcgcaggcc gctggcgtgg cgtggggtga
     7081 agccgatatc actgcgatca aacgtctttc cgatatgggc ttcaaagtca ccgtcaccgg
     7141 tggcctggcg ctggaagatc tgccgctgtt caaaggcatt ccaattcatg tgtttatcgc
     7201 cggtcgcagc atccgtgatg ccgcttctcc ggttgaagcc gcgcgtcagt tcaaacgttc
     7261 catcgccgaa ctgtggggct aaggagcgga tatgttgtcc aaacaaatcc cgcttggcat
     7321 ctatgaaaaa gcgctccccg ccggggagtg ctggctggaa cgcctgcgac tggcaaaaac
     7381 gttgggcttc gattttgtcg agatgtcggt agatgaaact aacgagcgcc tgtcgcgcct
     7441 cgactggagc cgcgaacagc gtctggcgct ggtgaatgct attgttgaaa ccggcgtgcg
     7501 tgtgccgtcc atgtgccttt ctgcccatcg tcgtttcccg ttgggcagcg aagatgacgc
     7561 ggtgcgggcg caggggctgg agattatgcg taaagccatc cagttcgccc aggacgttgg
     7621 cattcgcgtg atccagctgg cgggctatga cgtttactat caggaagcca ataacgaaac
     7681 gcgtcgtcgt ttccgtgacg gcctgaaaga gagcgttgag atggcgagcc gcgcgcaggt
     7741 gacgctggcg atggagatca tggattatcc gttgatgaac tccatcagca aggcgctggg
     7801 ttacgcgcac tatctcaaca acccgtggtt ccagctctac ccggatatcg gcaacctgtc
     7861 ggcgtgggac aacgatgtgc agatggagtt gcaggccgga atcgggcata tcgtcgcggt
     7921 gcatgtgaaa gacaccaaac cgggcgtctt caaaaacgtg ccatttggcg aaggtgtggt
     7981 ggatttcgaa cgttgcttcg aaacgctcaa acagagtggc tattgcgggc cgtacctgat
     8041 tgagatgtgg agcgaaacgg cggaagaccc ggcggcagaa gtggtgaaag cgtgtgattg
     8101 ggtgaaagcg cgcatggcca aagcaggcat ggtggaggcg gcataatgca aaagctaaaa
     8161 cagcaggtgt ttgaagccaa catggatctg ccgcgctatg ggctggtgac ctttacttgg
     8221 ggcaacgtca gcgctatcga ccgcgaacgc gggctggtgg tgatcaagcc cagcggcgtt
     8281 gcctacgaaa ccatgaaagc agacgatatg gtggtggtcg atatgagcgg caaggtggtg
     8341 gaaggggagt atcgcccatc ttccgacact gcgacgcatc tcgaactcta ccgtcgttac
     8401 ccgtcgcttg gcggcattgt ccatacccac tccactcatg ccaccgcatg ggcgcaggcg
     8461 gggctggcga tcccggcatt aggcaccacg cacgctgact acttctttgg tgatatcccg
     8521 tgtacgcgtg gattaagcga agaagaggtg cagggtgagt atgaactgaa cactggcaaa
     8581 gtgattatcg aaacgctggg caacgccgag ccgctgcata cgccgggaat tgtggtgtat
     8641 cagcacggac cgttcgcctg ggggaaagat gcccacgacg cggtgcataa cgcggtagtg
     8701 atggaagaag tggcgaaaat ggcgtggatt gcgcgcagta ttaatccaca actcaatcat
     8761 atcgacagct acctgatgaa taaacacttc atgcgtaagc acggtcctaa cgcttattac
     8821 gggcagaagt aaaacacgcg ctgcggaaat ttccttcctc gggagataac tggtctaatt
     8881 ccgcagccgt ttttcaaaaa aagccccctg cgaagggggc aaagcaaact atggcaatgt
     8941 ttcgttggtt atacctggtg ctagcgataa atatccgcgc tggcgtgcat attgccgtta
     9001 ctccccggtt cccgcatcag aattacgtgg tagtacgttg cgccttgcgc atctgtttct
     9061 tcatttaatg cctgacgtgc ttcgctttca gtggcgaaat tatgattgat ataaatcacg
     9121 cctaagcttt gcacatcgtc catatttctg gcctggtggt tattaatttc aatggctgcc
     9181 catgtatttg cacttagcaa aagcacagcc agaagggcta aaactcgact gaacatagat
     9241 acctcctcga cggctgactt tgtgtgctct cctctgtgat gatcttctga tttaatttta
     9301 atcaatgata aagaagttga tggtgaccat ttctgatgca gttgttcaaa aaaacactgt
     9361 gacggaatgt gatgaacttc aaatcagcgg tttagaggtt atttgcgaaa ggggagattt
     9421 atttctgctc cgcccttgag tttagcgggg tatgccagta ctataacgcg tcatttttca
     9481 gccgaccttt aacacgttcc ttgcctcccc gggattcggc tgacccagac aggaggctga
     9541 ataatccgta aggagcaatt cgatgcgtca ttacgaaatc gtttttatgg tccatcctga
     9601 ccagagcgaa caggttccgg gcatgatcga gcgctacact gctgccatca ctggtgcaga
     9661 aggcaagatc caccgtctgg aagactgggg ccgccgtcag ctggcttacc cgatcaacaa
     9721 actgcacaaa gcacactacg ttctgatgaa cgttgaagct ccgcaggaag tgatcgatga
     9781 gctggaaact accttccgct tcaacgatgc cgttatccgc agcatggtta tgcgtaccaa
     9841 gcacgctgtt accgaagcat ctccgatggt taaagcgaaa gacgagcgcc gtgagcgtcg
     9901 cgatgatttc gcaaacgaaa ccgctgatga tgctgaagct ggggattctg aagagtaatt
     9961 tctgatgacc aaccgtctgg tgttgtccgg caccgtgtgc aggactcccc ttcgaaaggt
    10021 cagtccatca ggaattcctc actgccagtt cgtgcttgag catcgttctg tgcaggagga
    10081 agccggcttt caccggcagg catggtgtca aatgcccgtt attgttagcg gacacgaaaa
    10141 ccaggccatt actcacagta taacggtcgg cagtcgcata accgttcagg ggttcatttc
    10201 atgccacaag gcaaagaacg gactgagcaa aatggttttg catgccgagc agattgaatt
    10261 gatagattct ggagactagc catatggcac gttatttccg tcgtcgcaag ttctgccgtt
    10321 tcaccgcgga aggcgttcaa gagatcgact ataaagatat cgctacgctg aaaaactaca
    10381 tcaccgaaag cggtaagatt gtcccaagcc gtatcaccgg tacccgtgca aaataccagc
    10441 gtcagctggc tcgcgctatc aaacgcgctc gctacctgtc cctgctgccg tacactgatc
    10501 gccatcagta atcggtcacg gtccattaat acgactttga gaggataagg taatgcaagt
    10561 tattctgctt gataaagtag caaacctggg tagcctgggt gatcaggtaa acgttaaagc
    10621 gggctatgct cgtaacttcc tggtaccgca gggtaaagct gttccagcta ccaagaaaaa
    10681 cattgaattc ttcgaagcac gtcgcgctga actggaagct aaactggctg aagttctggc
    10741 agctgctaat gctcgcgctg agaaaatcaa tgcactggaa actgttacca tcgcgtctaa
    10801 agctggcgac gaaggtaaac tgttcggttc catcggtact cgcgacatcg ctgacgctgt
    10861 aactgcagct ggcgttgaag tggctaagag cgaagttcgt ctgccgaacg gcgttctgcg
    10921 taccactggc gaacacgaag tgagcttcca ggttcacagc gaagtattcg cgaaagtgat
    10981 cgtaaacgta gtagctgaat aattcgttat tcaacgagac gtaaaaacgc cgaccattgg
    11041 tcggcgtttt gctttctatt tttcgtcagg cattaatttc gcaagtagat ccattcacca
    11101 ttctcttgtg tccataagtc aggatggttt gctggcgtta gatgcatacg atcactcctt
    11161 atgtctcggt cctgcggcac tcgctcgaaa cctaaacccg agtagattgc ggctgaattt
    11221 gataaggaac gtagaccaat tttcaaatca tggggtaaat cagaatacgc gtttagcaat
    11281 gcagcaatga tgaatccgag ggcattagaa acgcctgtaa cagtgtgaag ttggtcgata
    11341 taaagatcga ttgttggtag ttcagatgag cagcaattag gcatttgggc aggaagggtt
    11401 acaacgtatc ctatcgtttg gccggtatag tctggagtac tataaccagg tcccaaaaca
    11461 acctcgtgta ttgtgtattt actgttgggt tcgaaggatt cgacatcttg caataaatat
    11521 ttcagttctg cggttaacct cgcacccgcg taagctttat tgtatttctg ggcgtgaata
    11581 ttatctaatg tagcttcatg gttccatcca aaagaattat caccattcat tgttactgaa
    11641 tcgccacggg tttaacagac acctcagagt catttaagat gg
//
LOCUS       DADLTY010000136        11655 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.136, whole genome shotgun sequence.
ACCESSION   DADLTY010000136 DADLTY010000000
VERSION     DADLTY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.136"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            597..794
                     /locus_tag="JN334_003088"
     CDS             597..794
                     /locus_tag="JN334_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439148.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="JN334_003089"
     CDS             complement(744..1937)
                     /locus_tag="JN334_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7439149.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="JN334_003090"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="JN334_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HAY7439150.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="JN334_003091"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="JN334_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HAY7439151.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="JN334_003092"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="JN334_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HAY7439152.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="JN334_003093"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="JN334_003093"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HAY7439153.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="JN334_003094"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="JN334_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HAY7439154.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            10955..>11655
                     /locus_tag="JN334_003095"
     CDS             10955..>11655
                     /locus_tag="JN334_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7439155.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQW"
BASE COUNT         2956 a         2897 c         2992 g         2810 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10981 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    11041 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    11101 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    11161 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    11221 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    11281 cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    11341 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    11401 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggcaga
    11461 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    11521 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11581 atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga
    11641 ggtcacccaa tggga
//
LOCUS       DADLTY010000137        11577 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.137, whole genome shotgun sequence.
ACCESSION   DADLTY010000137 DADLTY010000000
VERSION     DADLTY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.137"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..1618
                     /gene="ftsK"
                     /locus_tag="JN334_003096"
     CDS             <1..1618
                     /gene="ftsK"
                     /locus_tag="JN334_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY7439156.1"
                     /translation="QQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHK
                     PTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRF
                     ELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREV
                     LDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSML
                     YKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLM
                     SALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFAD
                     LMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSK
                     IDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQ
                     YVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAA
                     RIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            1773..2384
                     /gene="lolA"
                     /locus_tag="JN334_003097"
     CDS             1773..2384
                     /gene="lolA"
                     /locus_tag="JN334_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAY7439157.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            2395..3738
                     /locus_tag="JN334_003098"
     CDS             2395..3738
                     /locus_tag="JN334_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAY7439158.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            3829..5121
                     /gene="serS"
                     /locus_tag="JN334_003099"
     CDS             3829..5121
                     /gene="serS"
                     /locus_tag="JN334_003099"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAY7439159.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            5361..7804
                     /gene="dmsA"
                     /locus_tag="JN334_003100"
                     /pseudo
     CDS             5361..7804
                     /gene="dmsA"
                     /locus_tag="JN334_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            7815..8432
                     /gene="dmsB"
                     /locus_tag="JN334_003101"
     CDS             7815..8432
                     /gene="dmsB"
                     /locus_tag="JN334_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY7439160.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            8434..9225
                     /locus_tag="JN334_003102"
     CDS             8434..9225
                     /locus_tag="JN334_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY7439161.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(9261..9887)
                     /locus_tag="JN334_003103"
     CDS             complement(9261..9887)
                     /locus_tag="JN334_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7439162.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            10202..11350
                     /locus_tag="JN334_003104"
     CDS             10202..11350
                     /locus_tag="JN334_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7439163.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         2763 a         2800 c         3125 g         2889 t
ORIGIN      
        1 tcaacagccg caacaaccgg ttgcaccgca gccgcagtat cagcagccac aacagccagt
       61 tgcgccacag ccgcaggata ccctgcttca tccgctgttg atgcgtaatg gcgacagccg
      121 tccgttgcac aaaccgacga cgccactgcc ttctctggat ttgctgacac cgccgccgag
      181 cgaagtggag ccggtagata cctttgcgct tgaacaaatg gcgcgtctgg tggaagcgcg
      241 tctggctgat ttccgtatta aagccgatgt cgttaattac tctccggggc cagttatcac
      301 tcgctttgaa cttaacctgg ctcctggcgt aaaagcggcg cgcatttcta acttgtcacg
      361 ggaccttgcc cgttcacttt cgacggtggc ggtgcgtgtc gttgaagtta ttcctggcaa
      421 accctatgta ggtctggagt taccaaataa aaaacgacaa accgtttatc tgcgcgaagt
      481 tttggataac gccaaattcc gcgataatcc gtcgccatta accgtggtgc tgggtaaaga
      541 tatcgccggt gagccggtgg ttgccgatct ggcgaaaatg ccgcacttgt tggttgcggg
      601 gactaccggt tccggtaaat ctgtcggtgt gaacgcgatg atcctgagca tgctttataa
      661 agcacagcca gaagatgtgc gtttcatcat gatcgacccg aaaatgctgg agctttcggt
      721 ttatgaaggc attccgcacc tgttaacgga agtcgttact gatatgaaag atgccgccaa
      781 cgcgctgcgc tggtgtgtta acgagatgga gcgtcggtat aaactgatgt ctgcgctggg
      841 tgtgcgtaat ctggcgggtt ataacgaaaa aattgctgaa gccgatcgca tgatgcgtcc
      901 gattccagac ccgtactgga agccgggtga cagtatggat gcccagcatc cggtgctgaa
      961 aaaagaacca tacattgtcg tgttggttga cgaatttgcc gacctgatga tgacggtagg
     1021 taaaaaagtg gaagagctga tagctcgtct ggcgcaaaaa gcccgtgccg cgggtatcca
     1081 cctcgtactg gcaactcagc gtccatcggt tgatgttatt actggtctga ttaaagcgaa
     1141 tattccgacc cgtatcgcct ttaccgtatc cagtaagatt gactcacgta ccattcttga
     1201 tcaggctggc gcggaatcac tgctgggtat gggggatatg ctctactctg ggccgaactc
     1261 cacgttgccg gtacgtgtcc atggtgcttt tgttcgcgat caggaagttc atgccgtggt
     1321 gcaggactgg aaagcgcgtg gtcgcccaca gtatgttgat ggcatcacct ccgacagcga
     1381 aagcgaaggt ggtgcgggtg gtttcgatgg cgctgaagaa ctggatccgt tgttcgatca
     1441 ggcggtgcag tttgtcactg aaaaacgcaa agcgtcaatt tctggcgtac agcgtcagtt
     1501 ccgcattggt tataaccgtg cagcgcgtat tatcgaacag atggaagcgc aggggattgt
     1561 cagcgaacag gggcacaacg gtaatcgtga agtgctggcc ccaccgccgt ttgactaatt
     1621 aatgcattgc cggataaggc gcggtagcgt cgcatccggc actctatcaa ctgaaaattc
     1681 agtattttct tctttcctca agctgattat tagcctggaa tagagagtag agggaactcc
     1741 cgatcgggag tgacgtaatt tgaggaataa tgatgaaaaa aattgctatc acctgtgcat
     1801 tactctcaag cttagtagca agcagcgttt gggctgatgc cgcaagcgat ctgaaaagcc
     1861 gcttggataa agtcagcagc ttccacgcca gcttcacaca aaaagtgact gacggtagcg
     1921 gcgcggcggt gcaggaaggt cagggcgatc tgtgggtgaa acgtccaaac ttattcaact
     1981 ggcatatgac acaacctgat gaaagcattc tggtttctga cggtaaaaca ctgtggttct
     2041 ataacccgtt cgttgagcaa gctacggcaa cctggctgaa agatgccacc ggtaatacgc
     2101 cgtttatgct gattgcccgc aaccagtcca gcgactggca gcagtacaat atcaaacaga
     2161 atggcgatga ctttgtcctg acgccgaaag ccagcaatgg caatctgaag cagttcacca
     2221 ttaacgtggg acgtgatggc acaatccatc agtttagcgc ggtggagcag gacgatcagc
     2281 gcagcagtta tcagctgaaa tcccagcaaa atggggctgt ggatgcagcg aaatttacct
     2341 tcaccccgcc gcaaggcgtc acggtagatg atcaacgtaa gtagaggcac ctgagtgagc
     2401 aatctgtcgc tcgatttttc ggataatact tttcaacctc tggccgcgcg tatgcggcca
     2461 gaaaatttag cacagtatat cggtcagcaa catttgctgg ctgcggggaa gccgttgccg
     2521 cgcgctatcg aagccgggca tttgcattct atgatcctct gggggccacc gggtaccggc
     2581 aaaacaaccc tcgctgaagt gattgcccgc tatgcgaacg ctgatgtgga acgtatttct
     2641 gccgtcacct ctggcgtgaa agagattcgc gaggcgatcg agcgcgcccg gcaaaaccgc
     2701 aatgcaggtc gccgcactat tctttttgtt gacgaagttc accgtttcaa caaaagccag
     2761 caggatgcat ttctgccaca tattgaagac ggcaccatca cttttattgg cgcaaccact
     2821 gaaaacccgt cgtttgagct taattcggca ctgctttccc gtgcccgtgt ttatctgttg
     2881 aaatccctga gtacagagga tattgagcaa gtactaactc aggcgatgga agacaaaacc
     2941 cgtggctatg gtggtcagga tattgttctg ccagatgata cacgacgtgc cattgcagaa
     3001 ctggtgaatg gcgacgcgcg ccgggcgtta aatacgctgg aaatgatggc ggatatggcc
     3061 gaagtctatg atagcggtaa gcgggtcctg aagcctgaat tactgaccga aatcgccggt
     3121 gaacgtagcg cccgctttga taacaaaggc gatcgctttt acgatctaat ttccgcactg
     3181 cataagtcgg tacgtggtag cgcacccgat gcggcgctgt actggtatgc gcgaattatt
     3241 accgctggtg gcgatccgtt atatgtcgcg cgtcgctgtc tggcgattgc gtctgaagac
     3301 gtcggtaatg ccgatccacg ggcgatgcag gtggcaattg cggcctggga ttgctttact
     3361 cgcgttggcc cggcggaagg tgaacgcgcc attgctcagg cgattgttta cctggcctgc
     3421 gcgccaaaaa gcaacgctgt ctacactgcg tttaaagccg cgctggccga tgctcgcgaa
     3481 cgcccggatt atgacgtgcc ggttcatttg cgtaatgcgc cgacgaaatt aatgaaggaa
     3541 atgggctacg ggcaggaata tcgttatgct catgatgaag caaacgctta tgctgccggt
     3601 gaggtttact tcccgccgga aatagcacaa acacgctatt atttcccgac aaacaggggc
     3661 cttgaaggca agattggtga aaagctcgcc tggctggctg aacaggatca aaatagcccc
     3721 ataaaacgct accgttaatg ttatcgttgc ggtaatgttg ttactgtatc cctgtggtcg
     3781 caggctgtgg ccacatctcc catttaattc gataagcaca ggataagcat gctcgatccc
     3841 aatctgctgc gtaatgagcc agacgcagtc gctgaaaaac tggcacgccg gggctttaag
     3901 ctggatgtag ataagctggg cgctcttgaa gagcgtcgta aagtattgca ggtcaaaacg
     3961 gaaaacctgc aagcggagcg taactcccga tcgaaatcca ttggccaggc gaaagcgcgc
     4021 ggggaagata tcgagccttt acgtctggaa gtgaacaaac tgggcgaaga gctggatgca
     4081 gcaaaagccg agctggatgc tttacaggct gaaattcgcg atatcgcgct gaccatccct
     4141 aacctgcctg cagatgaagt gccggtaggt aaagacgaaa atgacaacgt tgaagtcagc
     4201 cgctggggta ccccgcgtga gtttgacttt gaagttcgtg accacgtgac gctgggtgaa
     4261 atgcactctg gcctcgactt tgcagctgca gttaagctga ctggttcccg ctttgtggta
     4321 atgaaagggc agattgctcg catgcaccgc gcactgtcgc agtttatgct ggatctgcat
     4381 accgaacagc atggctacag tgagaactat gttccgtacc tggttaacca ggacacgctg
     4441 tacggtacgg gtcagctgcc gaaatttgct ggcgatctgt tccatactcg tccgctggaa
     4501 gaagaagcag acaccagtaa ctatgcgctg atcccaacgg cagaagttcc gctgactaac
     4561 ctggtgcgcg gtgaaatcat cgatgaagat gatctgccaa ttaagatgac cgcccacacc
     4621 ccatgcttcc gttctgaagc cggttcatat ggtcgtgaca cccgtggtct gatccgtatg
     4681 caccagttcg acaaagttga aatggtgcag atcgtgcgcc cagaagactc aatggcggcg
     4741 ctggaagaga tgactggtca tgcagaaaaa gtcctgcagt tgctgggcct gccgtaccgt
     4801 aaaatcatcc tttgcactgg cgacatgggc tttggcgctt gcaaaactta cgacctggaa
     4861 gtatggatcc cggcacagaa cacctaccgt gagatctctt cctgctccaa cgtttgggat
     4921 ttccaggcac gtcgtatgca ggcacgttgc cgcagcaagt cggacaagaa aacccgtctg
     4981 gttcataccc tgaacggttc tggtctggct gttggtcgta cgctggttgc agtaatggaa
     5041 aactatcagc aggctgatgg tcgtattgaa gtaccagaag ttctgcgtcc gtatatgaac
     5101 ggactggaat atattggcta atacccaatt tttctgaatc taaaaagcgc ctttagggcg
     5161 ctttttttgt ctccctttga taccgaacaa taattactcc tcacttacac gtaatactac
     5221 tttcgagtga aaatctacct atctctttga ttttcaaatt attctatgta tacaagccta
     5281 tatagcgagc tgctatagaa ataattacac aatacggttt gttactggaa tcaatcgtga
     5341 tcaagcttga gtgagccatt atgaaaacga aaatccctga tgcggtattg gctgctgagg
     5401 agagtcgccg tggtttggta aaaacgacag cgatcggcgg cctggcaatg gccagcagcg
     5461 cattaacatt accttttagt cggattgcgc acgctgtcga tagcgccatt ccaacaaaat
     5521 cagacgaaaa ggttatctgg agcgcctgta cagttaactg tggtagtcgc tgcccgctac
     5581 gtatgcacgt cgtggacggt gaaatcaaat atgtcgaaac gacaataccg gcgatgacaa
     5641 ttacgacggc ctacactagg ttcgcgcctg cctgcgtggg cgttccatgc gtcaccgtgt
     5701 ctacaatccg gaccgcctga aatatccgat gaaacgagtc ggggcgcgcg gtgaaggcaa
     5761 attcgagcgc attagctggg aagaagccta cgacatcatc gcgaccaata tgcagcgcct
     5821 gatcaaagag tacggcaacg agtctatcta tctgaactat ggcaccggta cgctgggcgg
     5881 caccatgacc cgctcctggc cgccgggaaa taccctggtc gcgcgcctga tgaactgctg
     5941 cggcggttat cttaaccatt acggtgacta ctcttccgcg caaattgcgg aaggtttgaa
     6001 ccatacctac ggcggctggg cagatggtaa cagcccgtcg gatatcgaaa acagtaaatt
     6061 ggtagtgctg tttggtaata acccaggcga aacgcgaatg agtggcggtg gggtgactta
     6121 ctatcttgaa caggcacgcc agaaatctaa tgctcgcatg atcatcatcg atccgcgcta
     6181 taccgacacc ggtgccgggc gcgaagatga gtggatccct attcgtccag gaacagatgc
     6241 cgcactggtt aacggtctgg cgtacgtcat gatcactaaa aacctggtgg atcaggcatt
     6301 cctcgataaa tattgcgttg gctatgacga gaaaaccctg cccgccagtg cgccgaaaaa
     6361 tggtcattat aaagcttata ttctgggtga agggccagat ggcgtggcta aaacaccgga
     6421 atgggcctcg caaatcaccg gtgttccggc agacaaaatc atcaaactgg ctcgtgaaat
     6481 cggcagtacc aaaccggcgt ttatcagcca gggatggggc ccgcagcgtc acgctaacgg
     6541 tgaaatcgca acccgtgcta tctcgatgct ggcgattctg accggtaacg ttggtattaa
     6601 tggagggaac agcggcgcgc gtgaaggttc atacagctta ccgtttgtcc gtatgccgac
     6661 cttggaaaac ccgatccaga ccagcatttc gatgtttatg tggaccgatg ccattgaacg
     6721 tggcccggaa atgacggcgc tgcgtgatgg tgtgcgcggg aaagataagc tggatgtgcc
     6781 gatcaaaatg atctggaact atgccggtaa ctgcctgatt aaccagcatt ctgaaatcaa
     6841 ccgtacccat gaaatcctcc aggatgataa gaagtgcgag ctgattgtgg ttatcgactg
     6901 ccacatgacc tcatcggcga aatatgctga catcctgctg cctgactgca ccgcttccga
     6961 acagatggac tttgcactgg atgcatcctg cgggaatatg tcttacgtga ttttcaacga
     7021 tcaggtgatt aaaccgcgct ttgaatgtaa gaccatctat gaaatgacca gcgaactggc
     7081 aaaacgtctt ggcgttgagc aacagtttac tgaaggccgt acccaggaag agtggatgcg
     7141 gcatctgtat gcccagtcgc gggaagcgat tcctgaactg ccaacgtttg aagagttccg
     7201 caagcagggg atctttaaaa agcgcgaccc acaagggcat cacgttgctt ataaagcctt
     7261 ccgtgaagat ccgcaggcaa acccactgac tacgccatcg ggcaaaattg agatttattc
     7321 gcaggcgctg gctgacattg ccgctacctg ggaattgcca gaaggcgatg tgatcgatcc
     7381 actgccgatc tacacgccgg gctttgaaag ttatcaggat ccgctgaaca aacagtatcc
     7441 gctgcagctt acaggtttcc actataaatc tcgcgttcac tcaacttacg gcaacgttga
     7501 tgtgctgaaa gcggcttgcc gtcaggaaat gtggatcaac ccgcttgatg cccaaaaacg
     7561 cggtatccac aacggcgata aagtcaggat ctttaacgat cgtggtgagg ttcatattga
     7621 ggcgaaagtg acgccacgaa tgatgccggg cgtggtcgca ctgggtgaag gcgcctggta
     7681 tgacccggat gcaaaacgtg tcgataaggg cggttgtatc aacgtactga ccactcaacg
     7741 tccgtctcct ctcgctaagg ggaatccgtc acatacaaac cttgttcagg ttgaaaaggt
     7801 gtaaggagta accgatgaca acccagtatg gattttttat tgattccagc cgttgcaccg
     7861 gttgcaaaac ctgcgagctg gcttgtaaag actacaaaga tttgacgcca gaagtcagtt
     7921 tccgccgcat ttatgaatat gctggcggcg actggcagga agataacggt gtctggcacc
     7981 agaacgtgtt tgcctactat ctgtcgattt cgtgcaacca ctgcgaagat ccggcttgta
     8041 ctaaagtctg cccgagcggt gcgatgcata aacgcgaaga tggttttgtt gtggtcgatg
     8101 aagatgtatg cattggctgc cgttactgtc atatggcttg cccgtatggc gcaccgcaat
     8161 ataacgaaac gaaaggccat atgaccaaat gcgatggttg ttatgaccgt gttgcggagg
     8221 gtaaaaagcc gatctgtgtt gaatcttgcc cgctgcgggc gctggatttc ggccctatcg
     8281 acgagctgcg taaaaaacat ggcgatctgg cggccgttgc gccgttgccg cgagctcact
     8341 ttaccaaacc gaatattgtg atcaaaccca atgccaatag ccgcccgacc ggggatacca
     8401 ctggctatct ggcaaacccg aaggaggtgt gagatgggaa gtggatggca tgaatggccg
     8461 ctgatgatct tcacggtctt cgggcaatgt gtggcgggtg gttttatcgt tctggctttg
     8521 gcgctgctca aaggcgacct gcgagcagaa gcccagcagc gtgttatcgc ctgcatgttt
     8581 ggcttatggg tactgatggg cattggcttt atcgcctcta tgctccatct tggttcaccg
     8641 atgcgcgcct ttaactcgct caaccgggta ggggcttcag cactcagtaa cgaaatcgcc
     8701 agcggttcga tcttttttgc tgtaggcggc atcggctggt tgctggcaat gctgaaaaag
     8761 ctgtcaccgg cattgcgtac gctatggctg gtggtgacga tggttcttgg cgtcgtcttt
     8821 gtctggatga tggtgcgtgt gtataacagc attgataccg ttccgacctg gtacagcatc
     8881 tggacgccga tgggctgggc gatgcgtctg ctgccagcca tttctgtact ggcactggtg
     8941 gtgagtggcg tgatgtcagt gatgcagggc acagagctgg caactattca tagttctgtg
     9001 cagcaggccg cagcgctggt gccggactat ggtgcgctga tgtcctggcg gatcgtgctt
     9061 ttggccgttg ccctgtgctt gtggattgca ccacagctaa aaggttatca gcctgcggtt
     9121 ccgctactat ccgtctcatt cattctgctt ctggcagggg aattaatcgg tcgcggcgta
     9181 ttctatggtt tgcatatgac cgtggggatg gccgtcgcaa gctaatcagt aacaaccggg
     9241 gtttcggccc cggttctctt ttatttctgc ttcgttaacg tgtcataact ggtcatcaaa
     9301 ttacgataat caggaatatg gttagagaac aacgtcgcca gtccttcaat atcattacgc
     9361 cagtcacggt gcagctcgca agccacgccg aaccatgtca tcaattgcgc accagcttgc
     9421 gacatgcgat cccacgccga atgccgggta atttcattaa aggtccctga tgcatcagtc
     9481 acgacaaaga catcaaaccc ttcttcaatg gctgaaagcg ccgggaatgc cacgcaaact
     9541 tcggttacca caccggcaat aattaactgt tttttacctg tcgctttgac tgcttttaca
     9601 aaatcttcgt tatcccaggc gttaatattg cccgggcgag caatgtaagg tgcatcgggg
     9661 aattgtgctt tcagttctag aactaatggg ccgttaggac cggtttcaaa actggtggta
     9721 agaatagttg gtaagttgaa atacttagcc aggtcaccca gcgccagcac gttattttta
     9781 aacttatcgg gttcgatatc ccgtacaagg gaaagtaaac cagcctggtg atcaacaagc
     9841 aaaacggcag catcattttt atcaagacga acatacggtt tggtcatcct cttctccttt
     9901 cgagagtcag tggtacgtct gaaatcggtc agacgcgatg cattgctctg aaagcataga
     9961 cgggaaatat gagtttgctg tgaccatgaa atttttcgac tgaacgaagc gtatgaaatt
    10021 tgtgttagat caataaaaac aatcagatga gattaatcat ctcttcagca ttagcgttat
    10081 gaatgagtaa tataaatcga taataccgcc gctgcgatat gcgcgtattt tcacccattg
    10141 acaatgtttt tggcggtggc atgatgcgca tgaaatttga acttcctcac ggttttaatt
    10201 catgtccacg tatacccggc ctgtcatgct tttgctgtct ggcctgcttt tgttgactct
    10261 ggcgatcgcg gtgttaaata cactcgtgcc gctttggctc gcccaggaac acatgtccac
    10321 atggcaggta ggcgttgtca gctcatccta ttttaccggc aaccttgtcg gtacattgct
    10381 gacagggtat gtcattaaac gcattggctt taaccgcagc tattatctgt cctccttcat
    10441 ttttgctgct ggctgtgccg gccttggcct gatgattggt ttctggagct ggttggcgtg
    10501 gcgttttgtc gcgggcgtcg gctgtgccat gatttgggtg gttgttgaga gcgcgctgat
    10561 gtgcagtggg acgtcacgta accgtgggcg tttgcttgct gcgtatatga tggtttatta
    10621 cgtgggaacg tttttaggcc agttactggt cagcaaagtc tcaaccgagc tgatgagcgt
    10681 attgccgtgg gttacaggtt tgacattggc agggatctta ccgctgttgt ttacgcgtgt
    10741 cctgaatcag caggctgaaa accatgattc gacgtcaatt acggcaatgc taaaactccg
    10801 tcaggcgcgg cttggcgtga atggctgcat tatctcagga atcgttctgg gatctctata
    10861 tggcctgatg cctctgtacc tcaatcacaa aggggtgagc aatgccagta ttggtttctg
    10921 gatggcggta ctggtcagtg cgggtatcct cggacaatgg ccgattggac gtctggcgga
    10981 taagtttggt cgactgctgg ttttgcgtgt tcaggtcttt gtcgtcattc tcggcagtat
    11041 tgcgatgctt agccaggcgg cgatggcccc agcgttattc atcctcggtg ccgctggctt
    11101 tacgctatat ccggtggcga tggcctgggc ttgtgagaaa gttgaacatc atcaactggt
    11161 ggcgatgaac caagccttac tgttgagcta taccgtggga agtctgcttg gcccgtcatt
    11221 tagcgctatg ctaatgcaga atttctccga taatttattg tttatcatga tcgccagcgt
    11281 atcgtttatc tatttgctga tgctgcttcg caacgccggt catacgccga aacccgttgc
    11341 tcacgtgtaa atgaattcaa gcaaagtgtg aacttactgt ttcacactct gcttttttgt
    11401 ttcttctatc tgacttgctt ttttccaaat tttattcgtt taaaaataaa atgtgcagca
    11461 ggttataatt ttgcatttcg ctatttccgc acttcttatt tgccgcgcat aatccctcgt
    11521 tttacagatg cccctttaat tttggcgaag gatttgtcta tggctgggaa tgttcag
//
LOCUS       DADLTY010000138        11197 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.138, whole genome shotgun sequence.
ACCESSION   DADLTY010000138 DADLTY010000000
VERSION     DADLTY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.138"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="JN334_003105"
     CDS             complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="JN334_003105"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HAY7439164.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(721..1749)
                     /locus_tag="JN334_003106"
     CDS             complement(721..1749)
                     /locus_tag="JN334_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HAY7439165.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1924..3516
                     /locus_tag="JN334_003107"
     CDS             1924..3516
                     /locus_tag="JN334_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAY7439166.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3526..4698
                     /gene="malY"
                     /locus_tag="JN334_003108"
     CDS             3526..4698
                     /gene="malY"
                     /locus_tag="JN334_003108"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HAY7439167.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4802..5803
                     /gene="add"
                     /locus_tag="JN334_003109"
     CDS             4802..5803
                     /gene="add"
                     /locus_tag="JN334_003109"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAY7439168.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5839..6884)
                     /locus_tag="JN334_003110"
                     /pseudo
     CDS             complement(5839..6884)
                     /locus_tag="JN334_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            7127..7252
                     /gene="blr"
                     /locus_tag="JN334_003111"
     CDS             7127..7252
                     /gene="blr"
                     /locus_tag="JN334_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HAY7439169.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7525..7740
                     /gene="ydgT"
                     /locus_tag="JN334_003112"
     CDS             7525..7740
                     /gene="ydgT"
                     /locus_tag="JN334_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HAY7439170.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7826..8266
                     /locus_tag="JN334_003113"
     CDS             7826..8266
                     /locus_tag="JN334_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HAY7439171.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8343..8924
                     /gene="rsxA"
                     /locus_tag="JN334_003114"
     CDS             8343..8924
                     /gene="rsxA"
                     /locus_tag="JN334_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAY7439172.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8924..9502
                     /gene="rsxB"
                     /locus_tag="JN334_003115"
     CDS             8924..9502
                     /gene="rsxB"
                     /locus_tag="JN334_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HAY7439173.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9495..>11197
                     /gene="rsxC"
                     /locus_tag="JN334_003116"
     CDS             9495..>11197
                     /gene="rsxC"
                     /locus_tag="JN334_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY7439174.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KA"
BASE COUNT         2603 a         2882 c         2818 g         2894 t
ORIGIN      
        1 tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa tgctattcac
       61 ccgaatattt ttttcaccca ggtcaaatgc catatttctg accagatgac tggccgcagc
      121 tttagatgat gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat
      181 ggtcagaata acgccacctc catttttttc catttctggc gcaacaagtt gtgacagatg
      241 gaaaaaagaa aacacattca gttcataagc acggcgaaaa tctgccattg gcatatcaaa
      301 cggtttagga ccaccgccac cggcgttgtt aaccaggata tcaactttac ccagattact
      361 gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacgccaggc
      421 aaatgcctga ccatccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt
      481 aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc
      541 aatacctgca cccgcacctg tgatgatggc gcattttccg tcgagtctca ggttgtcaga
      601 attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc ctgtgtgcca
      661 cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat aaagagagga
      721 ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg tctcttcatg
      781 ggtgattttt tgcatcatgc gatccgcaag tgtgatacca agttcccgcg ctggcgtgct
      841 ggcccaggta acaggtatat catcaagtgt ggttggtgtc gcatcggtaa atgccgccag
      901 cgaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc gccctgcttt
      961 cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca cggcactgat
     1021 ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct ggctggaagt
     1081 gcactccaac acccaatcgc tgtgaaacgg caggccaaat tttagtagag ttgcacaata
     1141 gccccccacc cgttctgcac gggttaatga ggaactttgc cctcccagcc aggcgatccg
     1201 ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca tgttgtccgg
     1261 gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca ccgggatagc
     1321 tttttcttct gccatccgcc gcaggtcatc gctgcttcct gcagccccgg caattaccac
     1381 accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct cgccgtcttt
     1441 accgccgtga agcaaaaaaa ccatccgtcc ctgtgcttcc agagcttccg tcaatccggc
     1501 cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa tgacgccgct
     1561 ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct cttcaatggc
     1621 ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccattaa gcaccagcga
     1681 aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta tttttttggc
     1741 ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt ttacctttta
     1801 tttatcttat acccgctatt atcgttgcgt aatgtgattt atgactcact aaaatttgat
     1861 aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac gaggagtcgt
     1921 tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca gcagttaggc
     1981 aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct cggcattggt
     2041 agttctctta gcagccatga tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg
     2101 caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt cctgcctgtc
     2161 atgttctgta tcgccatccc gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc
     2221 gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa
     2281 ggcattctgc caaccacaga tgccgccgtt ctgaaagcca ataacatcca gagcattctt
     2341 gggatccagt cgatcgatac cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg
     2401 atgctgcatg agcgtttcca taatatccgc ctgccggatg cgctggcatt cttcggcggt
     2461 acgcgcttcg taccaattat ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca
     2521 ttagtctggc cgattttcgc catgggtatt agcggcttgg gccatatgat aaacagcgcg
     2581 ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc gtttggtctg
     2641 catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca ggaggtctgc
     2701 ggtcaaaccg tcagcggcgc actgaccatc ttccaggcgc aattgagttg cccgaccact
     2761 cacggttttt ctgaaagcgt cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc
     2821 ggcggtctac caggtgcagc gttagctatg tatcactgcg cgcgcccgga aaatcgccat
     2881 aaaattaaag gtctgctgat ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa
     2941 ccgctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta
     3001 accggcctcg gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa taccgacggc
     3061 aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg
     3121 gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg tttcgctatc
     3181 acccgcttca atctgaaaac cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa
     3241 gccgttgccg gtgcgccggg taaatcaggt tacaacgttc ctgcaatcct cgaagcatta
     3301 ggcggtgccg acaatattgt cagcctcgat aactgcatta cccgtctgcg tttgtctgtg
     3361 gaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat tggcgtagta
     3421 caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc agtaaaagat
     3481 gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt cgatttttca
     3541 aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc tgaccgtttc
     3601 ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac tgccccctgc
     3661 attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag ccgctggaaa
     3721 aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta caccgcaatt
     3781 gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga actgattcgt
     3841 cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga cgcattttac
     3901 aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa gcaggctgac
     3961 ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga atgtaaaatt
     4021 atgctcctgt gtaacccaca gaatcctacc gggaaagtgt ggacgtgcga tgagctggag
     4081 atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga aatccatatg
     4141 gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg gggagactgg
     4201 gcgttgctga catcgggatc gaaaagtttc aatattcccg cgctgaccgg tgcttacggg
     4261 attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg
     4321 ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca gcaaggtgca
     4381 ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat ctcagataaa
     4441 atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta tctggcctgg
     4501 cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa
     4561 gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt
     4621 ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc
     4681 atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc aatcgtggat
     4741 ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa agagtgcgac
     4801 catgattgat accaccctgc cattaactga tatccatcgc caccttgatg gcaacattcg
     4861 tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg cacaatccct
     4921 tgaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg tgagctttct
     4981 gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc gccgcgtggc
     5041 atttgaaaac attgaagatg cagcccgtaa cggcctgcgc tatgtcgagc tgcgtttttc
     5101 accaggctac atggcaatgg cacataagct gcctgtagcg ggtgttgtcg aagcggtgat
     5161 cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta tcggcattat
     5221 gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt tagcccaccg
     5281 tgaccagatt accgcacttg atttagctgg tgatgaactt ggattcccgg gaagtctgtt
     5341 cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc atgcaggcga
     5401 agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg agcgtattgg
     5461 acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg agcaacaaat
     5521 tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag agctggctgc
     5581 acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca ctgacgatcc
     5641 cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag ctgctgggtt
     5701 atcccgtgag caaatccgcc aggcacagat taatggtctg gaaatggctt tcctcagcgc
     5761 agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat ggatggtgca
     5821 aattgcacca tccatttttc acgcaaggca caacgtcgcg cgatgtttgg cggattcgat
     5881 gcccagctca atcaactcca ttacctggat tgcctggctt gccggaaccg gattttcacc
     5941 atcgccattt aacgcatcac gaatcgccgc atagtaagcc ggataattcc caggcaccgt
     6001 caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc catcacgcat
     6061 atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt cttcctgtgg
     6121 atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc gtgctgactc
     6181 agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg acaagatggc
     6241 gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta ccgtcatgct
     6301 gaccggtaaa ccaaatagcg taatggcctg atcaagaaga tgcggtgcta aatcgtacca
     6361 gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg gacggaagcg
     6421 gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga gcaaacctaa
     6481 ccttttagcg tcaagaaatc gctatcccaa cggcggttat ggaatacaga cagcacacgc
     6541 cccaggcttt ttgccagcgc atccagctct cgcgcttgtg acagtgtcac ggtaaagggt
     6601 ttatcaacga ccacatgttt acccgcctca agcgccgctt tggctaacgg gaaatgggta
     6661 tcgttgggtg taggaatgac aatcaggtct atgttgggat cgttaaacag atgcttcggc
     6721 tcagagacaa ccgtaaccgt tggccagtcg gcttttactt ttgtttcatc actgctggag
     6781 attaccgcca gttccagccc gggcgtgccc gcaatcaggg gcgcatggaa ggttttgctc
     6841 gcataaccat acccaatcaa cccaacacgg atgttgtcgc tcatgtcatt gcctctcatt
     6901 gaagtatgat ggctatttga cactatcctt tacccacgct caacagttta ataacctgcc
     6961 agcaataagg gatgttgttt aacttaagtc aaaaaaatag cgaattttcc aacgacaaaa
     7021 gctaaatatc gcaaaaacct cagtaaaaat cttgctggag ctattattgc taagtaacat
     7081 ttaccccctg aagttaatgg atcaatcaag agagatgtgg gctgtaatga atcgtcttat
     7141 tgaattaaca ggttggatcg ttcttgtcgt ttcagtcatt cttcttggcg tggcgagtca
     7201 cattgacaac tatcagccac ctgaacagag tgcttcggta caacacaagt aagctctgca
     7261 cttgtggagc gacatgctgc ccgtccgggt gcatgttttc acttgtcgga tattaaacca
     7321 ggaatttatt atcttgttcg atgttgttgg tgattgtcag ggatagtaaa gcgcgttact
     7381 cttttcagag cagtttttca tccctgaata tttgccatca gcgcattctt attattgtaa
     7441 taacacaagc gttcactttt ggtgaaattt tatcatcact aataatacct acccctttgg
     7501 tattaattat tttatggacc ttttatgact gttcaggact acttattaaa atttcgcaaa
     7561 atcagttcac tcgaaagtct ggaaaaactc tacgaccatc ttaattacac cctgacggac
     7621 gatcaggaac tgatcaatat gtatcgtgct gccgatcacc gtcgcgcaga gctggtttct
     7681 ggcgggcgtt tgtttgacct cggccaggta ccgaagtccg tctggcacta tgtccaataa
     7741 agaaagtagc gatcggttat aaaagcctta taataacgcc cctaaaatta gatttgcctg
     7801 tttgcgtcgc tattctggag aacctatgac caccacaacg ccacaacgta ttggaggctg
     7861 gttgcttggc ccgttggcct ggcttttagt cgctctgttg agtacgacgc tggcgttgtt
     7921 gctgtatacc gctgcgttat cttctccgca aacatttcaa acgctcggcg gacaagcact
     7981 gacgacacaa atcttatggg gcgtttcttt tattaccgct atcgccatgt ggtattacac
     8041 actgtggttg accatcgctt tctttaaacg tcggcgttgt gttcctaaac actatattat
     8101 ctggctgctt atttccgtat tgctggcagt aaaagcattc gctttttcac ccgtggaagt
     8161 tggcatagcc gttcgccagt tgctgtttac tttgttggca accgcattga tcgtccccta
     8221 tttcaaacgc tcgtcgcggg taaaagcgac gtttgtgaat ccgtaataac cttacagtta
     8281 acctgttgtc gcctgctctg gattaacgga taataggcgg cttttttatt tcaggccgaa
     8341 aaatgactga ctacctgtta ctgtttgtcg gaactgtact ggtcaataac tttgtactgg
     8401 tcaagtttct cggtctctgt ccgtttatgg gggtttccaa aaaactggaa accgcgatgg
     8461 gcatggggct ggcaacaacg tttgtgatga cgctgacgtc tatttgcgcc tggcttatcg
     8521 atacgtggat tttgatccca cttaatctga tttacctgcg caccctggca tttattctgg
     8581 tgattgctgt ggtcgtgcag ttcaccgaga tggtggtgcg caaaaccagc ccggtgcttt
     8641 accgtctgct ggggattttt ttgccgctta tcaccaccaa ctgtgcagtg ctcggcgtgg
     8701 cgttgctgaa tatcaatctc gggcacaatt tcttgcagtc ggcgctgtac ggtttttccg
     8761 ccgctgtcgg tttctcgctg gtgatggtgc tcttcgccgc catccgcgaa cgccttgctg
     8821 tggctgatgt cccggcacct tttcgcggta atgccattgc gttaattacc gcaggtctta
     8881 tgtctctggc ctttatgggc tttagtggtt tggtgaagtt gtaatgaatg ctatctggat
     8941 tgccgttgcc gccgtgagcc tgctgggcct ggcgtttggc gccattctgg gttatgcctc
     9001 ccgccgtttt gcggtggaag acgatccggt cgttgagaaa attgacgaaa tcttaccgca
     9061 gagccagtgt ggtcagtgcg gttatcccgg ctgtcgcccc tacgcggaag ccatcagctg
     9121 taacggtgaa aaaatcaacc gttgcgcccc aggtggcgaa gctgtgatgc taaaaattgc
     9181 cgagttgctt aatgtcgagc cgcagccgct ggatggcgaa gcgcaagagc taacgcctgc
     9241 gcggatggtg gcggttattg atgaaaataa ctgtattggc tgcactaaat gtattcaggc
     9301 ttgtccggta gacgccatcg ttggcgctac ccgtgtcatg catacggtaa tgagtgatct
     9361 ctgtacgggc tgcaatttat gtgttgatcc gtgcccgacg cactgcatct cgttgcaacc
     9421 ggtcgcagaa acacctgact cctggaaatg ggatctgaac accattcccg tgcgtatcat
     9481 tcccgtggaa caccatgctt aagttattct ctgcattcag aaaaaataaa atctgggatt
     9541 tcaacggcgg catccatcca ccggagatga aaacccagac caacggtacc cccctgcgcc
     9601 aggtactact ggcgcagcat tttgttattc cactgaaaca gcatattggc gctgaaggtg
     9661 agttgtgcgt tagcgtcggc gataaagtat tgcgcggcca gccgcttacc cgtggtcgcg
     9721 gcaaaatgct gcctgttcac gcgcccacct cgggtaccgt tacggctatt gcgccccact
     9781 ctacggctca tccttcagct ttagctgaat taagcgtgat tattgatgcc gatggtgaag
     9841 actgctggat cccgcgcgac ggctgggtcg attatcgctc tcgcagtcgc gaagagttaa
     9901 tcgagcgcat acatcagttt ggtgttgccg ggctgggcgg tgccggattc ccgacaggcg
     9961 ttaaattgca gggtggtgga gataagattg aaacgttgat tatcaacgct gctgagtgcg
    10021 agccgtacat taccgccgat gaccgtttga tgcaggattg cgcggctcag gtcgtagagg
    10081 gtattcgcat tcttgcgcat attctgcagc cacgcgaaat tcttatcggc attgaagata
    10141 acaaaccgca ggcgatttcc atgctgcgcg cggtgctggc ggactctaac gatatttctc
    10201 tgcgggtgat tccaaccaaa tatccttctg gcggtgctaa acaattaacc tacattctga
    10261 ccgggaagca ggttccacat ggcgggcgtt catccgatat cggcgtatta atgcaaaacg
    10321 tcggcactgc ttatgcagtg aaacgtgccg ttattgatgg cgagccgatt accgagcgtg
    10381 ttgtaaccct gactggcgaa gcaatcgctc gcccgggcaa cgtctgggca cggctgggga
    10441 cgccagtgcg tcatttattg aatgatgccg gattctgccc ctctgccgat caaatggtga
    10501 ttatgggtgg cccgctaatg ggctttacct tgccatggct ggatgtcccg gtcgtaaaga
    10561 ttaccaactg tctgttggct ccctctgcca atgaacttgg cgaaccacag gaagaacaaa
    10621 gctgcatccg gtgtagcgcc tgtgctgacg cctgccctgt ggatcttttg ccgcaacagt
    10681 tgtactggtt cagcaaaggt cagcaacacg ataaagctac cacgcataac attgccgact
    10741 gcattgaatg tggggcttgc gcgtgggttt gcccgagcaa tattcccttg gtgcaatatt
    10801 tccgtcagga aaaagctgaa attgcggcta ttcgtcagga agaaaagcgc gccgcagaag
    10861 ccaaagcgcg tttcgaagcg cgccaggctc gtctggagcg cgaaaaagcg actcgccttg
    10921 aacgacataa gagcgcagcc gttcaacctg cagccaaaga taaagatgcg attgctgccg
    10981 ctctggcgcg ggtgaaagag aaacaggccc aggctacaca gcctattgtg attaaagcgg
    11041 gcgaacgccc ggataacagt gcaattattg cagaacggga agcccgtaaa gcgcaagcca
    11101 gagcgaaaca ggcagaactg gagcaaacta acgacgcagc aaccgttgct gaaccacgta
    11161 aaactgccgt tgaagcagct atcgcccgct ccaaagc
//
LOCUS       DADLTY010000139        11110 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.139, whole genome shotgun sequence.
ACCESSION   DADLTY010000139 DADLTY010000000
VERSION     DADLTY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.139"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="JN334_003117"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="JN334_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HAY7439175.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="JN334_003118"
     tRNA            complement(621..695)
                     /locus_tag="JN334_003118"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="JN334_003119"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="JN334_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="JN334_003120"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="JN334_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HAY7439176.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2932..3990)
                     /locus_tag="JN334_003121"
     CDS             complement(2932..3990)
                     /locus_tag="JN334_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439177.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="JN334_003122"
     CDS             complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="JN334_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HAY7439178.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6068..6352
                     /locus_tag="JN334_003123"
     CDS             6068..6352
                     /locus_tag="JN334_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HAY7439179.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6532..7842
                     /gene="fadI"
                     /locus_tag="JN334_003124"
     CDS             6532..7842
                     /gene="fadI"
                     /locus_tag="JN334_003124"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HAY7439180.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7842..9986
                     /gene="fadJ"
                     /locus_tag="JN334_003125"
     CDS             7842..9986
                     /gene="fadJ"
                     /locus_tag="JN334_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HAY7439181.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10189..10674
                     /gene="sixA"
                     /locus_tag="JN334_003126"
     CDS             10189..10674
                     /gene="sixA"
                     /locus_tag="JN334_003126"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAY7439182.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2775 a         2544 c         2749 g         3042 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     3121 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     3181 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     3241 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     3301 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     3361 ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt
     3421 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3481 gtccgttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3541 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3601 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3661 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3721 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3781 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3841 tagatgttta atacaggtgc ttattccttc cttaccatta aaaaggtttt tgaactcttg
     3901 tactaatcca ttaatttcat cattgttatt ttcattgatg gattctgggt aagctgcgct
     3961 ggtattgttg attgaaatag atggtagcat gattttattc catgttgatt gatttgttac
     4021 aaatatatat tacctaatga aatagactat caaaaaacgc tcatttattt agaacctatc
     4081 tttaatttga gtgtataagc gcacaatagc caggggggtt gagtatcctg gaaatgcaga
     4141 aaagtaaact ctttgagaag gacgagcaga acgcttgaaa atatgcattt cccttcaggt
     4201 ttgtctgtgg ataccactta ctgatttgaa agggcaagct gccatcaggc tgtttgcaaa
     4261 actcaggagg caagcaatga ggaaactact ttcggtgaag tgggggagtt aaagtcacct
     4321 gcatagcagg tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta
     4381 ccgaacagcc aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg
     4441 ctctgaccgt gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa
     4501 gtcgtacctg cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctga
     4561 gccggaactg ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag
     4621 taataagtgg tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac
     4681 agcgtgtcgc cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa
     4741 gccaggctat agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac
     4801 atttcaggca ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga
     4861 attggcaaac cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag
     4921 tcaattttca cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac
     4981 aggattccgg cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta
     5041 ttactgtcga taccgttggc ggtagctgcc aatgcttgcc cttgaggagt ttgcccggca
     5101 ggagattgca taatctgacc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt
     5161 ttcgcgcgag cgtagatggc gttgaaacca agaccaaagc tccatgcatt atttaagcga
     5221 tacgcaccgc ttaagttcag gttcatggtt tcaaggtcag ttgtaccccc gacagagccg
     5281 cctgcataag tatcattaaa ctctgttgcc agaccatagt tagaggtaat agaagcgccc
     5341 caaccaaatt ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc
     5401 gcgatgttat cggctttcag gctacgacca gacggagacg ttccgctgat attcacatcc
     5461 gggtcaatat aaaccgcacc cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg
     5521 gggttacggc taacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg
     5581 cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag
     5641 ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc
     5701 ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag
     5761 tgtaggggct gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag
     5821 ctagaatgtt gcaagtatgt ttccatcttt tttcaaatat gatctaagaa acgcgatcca
     5881 gctggcaaaa tttggaggtt gcgcactcgc ggattcgggt gaattttgtt ttagatcatt
     5941 tttaagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt
     6001 tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa
     6061 atcaacgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg
     6121 caccattatg gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc
     6181 agaagcagaa caaacgctgg cggcgctgac tgaaaaatcc cgtagcgtgg aatccgaacc
     6241 gtgcaaaatc tccccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt
     6301 cacttttgcc tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt aattcctctc
     6361 gggtacgtac gcccctgtca gttgctggca ggggcgtttt cttttctttc ttctgtcttc
     6421 actgtgtcat agctcccact ttcctttttc tccgcttggc taaatgtaaa aaaatggtta
     6481 agactatgat caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag
     6541 gttttaccac tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg
     6601 ccttttgccc gccaggcgac ggcttttcat ggcattccca cggttgattt agggaagatg
     6661 gtggtaggcg aactgctggc acgcagcgag atccccgctg aagtgattga acagctggtc
     6721 tttggtcagg tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt
     6781 accggaatga atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc taccagtttc
     6841 caagcggttg caaacgtcgc agaaagcctg atggcgggaa ctatccgagc ggggattgcc
     6901 ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgtacgtg
     6961 ctggttgatg tcaacaaagc tcgaaccatg agccagcgcc tgaaactctt ctctcgcctg
     7021 cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag aatattctac tggcttgcgg
     7081 atgggcgaca ccgccgaaca aatggcgaaa acctacggca tcacccgaga acagcaagat
     7141 gcattagcgc accgttcgca tcagcgtgcc gctcaggcat ggtcagacgg aaaactcaaa
     7201 gaagaggtga tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat
     7261 attcgcggta attcctcgct tgccgattac gcaaagctgc gcccggcgtt tgatcgcaaa
     7321 cacggaacgg taacggcggc aaacagtacg ccgctgaccg atggcgcggc agcggtgatc
     7381 ctgatgactg aatcccgggc gaaagaatta gggctggtgc cgctggggta tctgcgcagc
     7441 tacgcattta ctgcgattga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca
     7501 ccgctggcgc tggaacgtgc cggtttgacg atgagcgaac tgacattgat cgatatgcac
     7561 gaagcctttg cagctcagac actggcgaat attcagttgc tgggtagtga acgttttgct
     7621 cgtgaagtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg
     7681 cttggcggtt cgattgctta cggacatccc ttcgcggcga ccggcgcgcg gatgattacc
     7741 cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagttac cgcctgtgct
     7801 gccggtgggc ttggcgcggc aatggttctg gaggcggaat aatggaaatg gcatcagcgt
     7861 ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta ccggatgaga
     7921 aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg cgccattatt aagcaactcc
     7981 gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc gaaaccggac aattttatcg
     8041 ccggagcaga catcaacatg attggcaact gcaaaacggc gcaagaagcg gaagcactgg
     8101 cgcggcaggg ccaacagttg atggcggaga ttcatgcttt gcccattccg gttatcgcgg
     8161 ctattcatgg cgcttgcctg ggcggtgggc tggagctggc gctggcgtgc cacggtcgcg
     8221 tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac
     8281 ccggttcagg cggcacccag cgtttaccgc gtctgatagg cgtcagtaca gcattagaga
     8341 tgattctcac cggaaaacaa cttcgggcga aacaggcatt aaagctgggg ctggtggatg
     8401 acgttgttcc gcactccatt ttgctggaag ccgctgttga gctggcgaag aaggatcgcc
     8461 catcttcccg ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc
     8521 tgctgttcaa aatggtcggc aagaaaacag aacacaaaac tcaaggcaat tatccggcga
     8581 cagaacgcat cctggaggtt gttgaaacgg gattagcgca gggcaccagc agcggctatg
     8641 acgctgaagc tcgcgccttt ggcgaactgg cgatgacgcc gcaatcgcaa gctttgcgta
     8701 gtatcttttt tgccagtacg gacgtgaaga aagatcccgg tagtgatgca ccgcctgcgc
     8761 cattaaacag cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca
     8821 ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc
     8881 atgcgctgaa gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt catctcaaag
     8941 ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat cgcggctttg
     9001 cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg
     9061 tggcggaagt ggagcaaaat tgcgccgctc ataccatttt tgcttcgaac acgtcatctt
     9121 taccgattgg tgatatcgcc gctcacgccg cgcgacctga gcaagttatt ggcctgcatt
     9181 tcttcagtcc ggtggaaaaa atgccgctgg tagagattat tcctcatgcg ggtacatcgg
     9241 cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca gggcaaaacg cccattgtcg
     9301 tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatt aatgaagcta
     9361 tccgcatgct gaccgaaggt gaacgggtag agcacattga tgccgcgcta gtgaaatttg
     9421 gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa
     9481 ttattcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt
     9541 cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg
     9601 gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcctgc catttacccg ctcattggcg
     9661 cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc
     9721 tgaatgaagc agtacgttgt gttgatgagc aggttatccg tagcgtgcgt gacggggata
     9781 ttggtgcggt atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg
     9841 attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt
     9901 cccgttttac cccttgcgag cgtttggtcg agatgggcgc gcgtggggaa agtttttgga
     9961 aaacaactgc aactgacctg caataagaag gtcaaagcta tatgaatccg cgctgaatgg
    10021 cggagtgttg gtcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa
    10081 cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt
    10141 ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt
    10201 atcatgcgtc acggcgacgc agccctcgat tccgccagtg attcggttcg tcctctgacc
    10261 actaatggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa
    10321 atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg
    10381 gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat
    10441 gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg
    10501 gtgatctctc acttaccgtt agtcgggtat ctggttgccg agttatgtcc gggcgaaacg
    10561 ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc ttgatgaaag cggaaacggt
    10621 acattcaact ggcagatgag cccgtgtaat ctgaagatgg caaaagctat ctgatgtgtt
    10681 ccaaattatg cccaatgagg tagattcatt gggcataatt gctcattagg cagcggatat
    10741 ctgtcaacct cactactatc gaagactctc cttatctata aataaaacac tttctacatt
    10801 cattcggaaa ttattcttct acgcgtatct tgattttgcc caaagttaca tgttgaaatg
    10861 aataatatct gtttgacact cattgtcatt tattaataca gataatagag aaaatagagt
    10921 tgaataatca ggcaggatgt aattgaaact tgattaaatt ctggctatag tgtttatata
    10981 ttatattgtc aaaagtggat aaatgccaat cgcattatat gttcattgtt tgttttttaa
    11041 gctgttgata ttcattattg atatactttc tattttttct aaatacttat tccgtatttt
    11101 ttaaggatgt
//
LOCUS       DADLTY010000140        11069 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.140, whole genome shotgun sequence.
ACCESSION   DADLTY010000140 DADLTY010000000
VERSION     DADLTY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.140"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(82..1164)
                     /gene="lptG"
                     /locus_tag="JN334_003127"
     CDS             complement(82..1164)
                     /gene="lptG"
                     /locus_tag="JN334_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY7439183.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(1164..2264)
                     /gene="lptF"
                     /locus_tag="JN334_003128"
     CDS             complement(1164..2264)
                     /gene="lptF"
                     /locus_tag="JN334_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY7439184.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            2531..4042
                     /gene="pepA"
                     /locus_tag="JN334_003129"
     CDS             2531..4042
                     /gene="pepA"
                     /locus_tag="JN334_003129"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAY7439185.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            4176..4619
                     /gene="holC"
                     /locus_tag="JN334_003130"
     CDS             4176..4619
                     /gene="holC"
                     /locus_tag="JN334_003130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAY7439186.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            4619..7474
                     /gene="valS"
                     /locus_tag="JN334_003131"
     CDS             4619..7474
                     /gene="valS"
                     /locus_tag="JN334_003131"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAY7439187.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(7528..8724)
                     /locus_tag="JN334_003132"
     CDS             complement(7528..8724)
                     /locus_tag="JN334_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HAY7439188.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            8917..9420
                     /locus_tag="JN334_003133"
     CDS             8917..9420
                     /locus_tag="JN334_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7439189.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(9466..9882)
                     /gene="rraB"
                     /locus_tag="JN334_003134"
     CDS             complement(9466..9882)
                     /gene="rraB"
                     /locus_tag="JN334_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HAY7439190.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            10044..11057
                     /gene="argF"
                     /locus_tag="JN334_003135"
     CDS             10044..11057
                     /gene="argF"
                     /locus_tag="JN334_003135"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAY7439191.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         2943 a         2829 c         2784 g         2513 t
ORIGIN      
        1 cccaaagggg cgaggggacc gttcgaactc gtatttgact ttgtcaacaa taataaaggg
       61 agctttcgct cccttgttag attacgattt tctcattaac agccacaggc tgattaagaa
      121 gaagctggca cttggcaaca gtgcgccgat gatcggcggg atgccgtaaa ccaacgtcag
      181 cgggccgaag atctggtcca gtacgtagaa gacaaaaccg aagctgatac ctgtgaccac
      241 acgcacgccc atcggtacgc tacgcagtgg gccaaagata aacgacagcg ccatcagcat
      301 catcaccgcc acggatagcg gctggaagat tttgctccac atgttgagct gataacgtcc
      361 ggcatcctga ccgctcgact tcagatactt cacgtagttg tgcaaaccac tgatggagag
      421 tgcatccggg tccagcgcca ccacgcccag tttgtctggc gtgaggtcgg ttttccaggt
      481 gccgctcacc gtctgcgaac cggtaatctg tttcggattg gtcagatcag attcatcaac
      541 ctgcgacaga cgccagactt tatgttccgg gtcaaacttc gcggtagcgg cataacgtac
      601 ggattgcaga cgacgattct cgttaaaggc ataaatactg atgccaccta acacctcgtc
      661 acctttaacc cgctcaatgt agacgaagtt gttgccatct ttcgcccata aaccttgctg
      721 ggtagagagc aacgagccgc cgtacatcgc ctgcgcacgg tagttacgcg ccatctgttc
      781 gccctgcggc gcgacccatt cgccaatcgc catcgtcagc aagaccagtg gaatggcggt
      841 tttcatcacc gacagcgcta cctgcatacg ggtaaaacca gaagcctgca tcaccaccag
      901 ttcgctgcgc tgcgccagca tcccaagacc aagcaacgcc ccaagaagag ccgccatcgg
      961 gaagaagatc tgcacatctt tcggcacgct cagcaaggta tacattcctg cgcctaacgc
     1021 gtcgtaactc ccctgcccgg cttttttcag ctgatcgaca aatttgataa tgcccgacag
     1081 cgacaccagc atgaacagcg tcatcatgat ggtggtgaaa atagttttac cgatatagcg
     1141 gtcaagtacg ccaaaaggtt gcatcacacc gctcctttac gcgaaaaact ggcgcgcagg
     1201 cggcggaccg gcacggtgtc ccaaaggttg agaacaatcg ccaaagccag ataaatcagg
     1261 ttaacggtcc acatccacag tgtcgggtcc agcttacctt taccgccgtt cgatttcagg
     1321 gaggtctgga tcaggaagaa aagtagatac agcagcatgg ctggcagcat cgacagtacg
     1381 cgtccctgac gcgggttaac cacgctcaac ggtacaacca taagtgccat cataaacacg
     1441 gtgaatacca acgtgatacg ccagttcagt tctgcgcgag cacgatcggt gtcagtgttc
     1501 cacaatgtgc gcatgtccat ctggtcggta tcgttcgggt cgagcgccac cgcctggtga
     1561 ccaatgatcg cctgataatc ctggaaatcc gtaatgcgga aatcacgtaa cagtgcagtg
     1621 ccttcgaagc gcgttccctg gttgagagtg acgacctggg agccgtcgcg cagctgggtt
     1681 aaatgtccgg aatcggccac caccacagaa ggacgtgcat taccttttgg tcgaatttgt
     1741 gcgaggaaca catctttgaa atcgctgccg tcaacgcttt cgatgaacag caccgagctg
     1801 ccattagtcg cttgctggaa ttgcccttgc gccagcgccg ccatgccagg gttcgctttc
     1861 gcttctgcta acacttcatc ctgatgacgc gatgaccacg gtcccgccca catcacgtta
     1921 accgccgcga cgattgccgt gaataccgca aggatcattg ccgctttcac cagaaccgct
     1981 ttgctcaagc cgcaggcatg cattaccgta atttcacttt cggtatacag tttgcccagc
     2041 gtcatcagca gcccgaggaa caggcttaat ggcaggataa gctgcgccat ttccggcacg
     2101 cccaacccga gaagggagag caccagattc gccggaatat cgccgtcaac cgctgcgccg
     2161 aggatcctca ctaacttttg acagaagaag atcaaaagca agatgaagag tatcgccagc
     2221 tggcttttga gcgtctcccg caccagatat cttatgatta tcactttaaa tacgcccgta
     2281 aaaactcgtc ttttgcagga ttttagcttg tttcatggct taaacgtcat ttattctctt
     2341 gagtcgtcga aatcgtcgct aagataatta tactcaacgg attcacctct cagattttgt
     2401 tctgacgtgc caatgccgta ataacgttaa gattaacacg aagtcatcgc aacagcggac
     2461 atgagttacg aaagcttgca attctatctg tagccaccgc cgttgtcttt aagattcagg
     2521 agcgtagtgc atggagttta gtgtaaaaag cggtagcccg gagaaacagc ggagtgcctg
     2581 catcgtcgtg ggcgtcttcg aaccacgtcg cctttctccg attgcagaac agctcgataa
     2641 aatcagcgat gggtacatca gcgccctgct acgccggggc gaactggaag gaaaaccggg
     2701 gcagacattg ttgctgcacc atgttccgaa tgtactttcc gagcgaattc tccttattgg
     2761 ttgcggcaaa gaacgtgagc tggatgagcg tcagtacaag caggttattc agaaaaccat
     2821 taatacgctg aatgatactg gctcgatgga agcggtctgc tttctgactg aactgcacgt
     2881 taaaggccgt aacaactact ggaaagtgcg tcaggctgtc gagacagcaa aagagacgct
     2941 ctacagtttc gatcagttga aaacgaataa gagcgaaccg cgtcgtccgc tgcgtaaaat
     3001 ggtgttcaac gtgccgaccc gccgtgaact gaccagcggt gagcgcgcga tccagcacgg
     3061 tcaggcgatt gccgccggga taaaagcagc gaaagatctc ggtaatatgc cgccgaatat
     3121 ctgtaacgcc gcttatctcg cctctcaggc gcgccagctg gctgacagct acagcaagaa
     3181 tgtcatcacc cgcgttatcg gcgaacagca gatgaaagag ctgggaatgc actcttatct
     3241 ggcggtcggt cagggttcac aaaacgaatc gctgatgtcg gtgattgagt acaaaggcaa
     3301 cgcgtcggaa gatgcacgcc caatcgtgct ggtgggtaaa ggtttaacct tcgactccgg
     3361 cggtatctcc atcaagcctt cagaaggcat ggatgagatg aagtacgata tgtgcggcgc
     3421 ggcggcagtt tacggcgtga tgcgtatggt cgcggagctg caactgccga ttaacgttat
     3481 tggcgtgctg gcaggctgtg aaaacatgcc tggcgggcgt gcctatcgtc cgggcgatgt
     3541 gttaaccacc atgtccggtc aaaccgttga agtgctgaac accgatgccg aaggccgcct
     3601 ggtactgtgc gacgtgttaa cttacgttga acgttttgag ccggaagcag tgattgacgt
     3661 ggcgacgctg accggtgcct gcgtgatcgc gctgggtcat catattactg gtctgatgac
     3721 gaaccataat ccgctggccc atgaactgat tgccgcgtct gaacaatccg gtgaccgcgc
     3781 atggcgctta ccgctgggtg acgagtatca ggaacagctg gagtccaatt ttgccgatat
     3841 ggcgaacatt ggcggtcgtc ctggtggggc gattaccgca ggttgcttcc tgtcacgctt
     3901 tacccgtaag tataactggg cgcacctgga tattgcagga accgcctggc gttctggtaa
     3961 agcaaaaggc gcaaccggtc gtccggtagc gttgctggca cagttcctgt taaaccgcgc
     4021 tgggtttaac ggcgaagagt aagtttgcgt tttaccctca ccctaaccct ctccctaagg
     4081 gagagggaac cgatcgagcg caatagaaat tcattgctca tctttcaggg tgaggaatgc
     4141 agcaatattt aaatccacca caagaagccc catatatgaa aaacgcaacg ttctaccttc
     4201 tggacaatga caccaccgtc gatggcttaa gcgccgttga gcaactggtg tgtgaaattg
     4261 ccgcagaacg ttggcgcagc ggtaagcgcg tactcatcgc ctgtgaagat gaaaagcagg
     4321 cttaccggct ggatgaagcc ctgtgggcgc gtccggcaga aagctttgtt ccgcataatt
     4381 tagcgggaga aggaccgcgc ggcagtgcgc cggtggagat cgcctggccg caaaaacgca
     4441 gcagcagccc gcgcgatata ttgattagtc tgcgaacaag ctttgcagat tttgccaccg
     4501 ctttcacaga agtggtagac ttcgttcctt atgaagattc tctgaaacaa ctggcgcgcg
     4561 aacgctataa agcctaccgc gtggctggtt tcaacctgaa tacggcaacc tggaaataat
     4621 ggaaaagaca tacaacccac aagatatcga acagccgctt tacgagcact gggaaaagca
     4681 gggctacttt aagcctaatg gcgatgaaag ccaggaaagt ttctgcatca tgatcccgcc
     4741 gccgaacgtc accggcagtt tgcatatggg tcacgccttc cagcaaacca tcatggatac
     4801 catgatccgc tatcagcgca tgcagggcaa aaacaccctg tggcaggtcg gtactgacca
     4861 cgccgggatc gctacccaga tggtcgttga gcgcaagatt gccgcagaag aaggtaaaac
     4921 ccgtcacgac tacggccgcg aagctttcat cgacaaaatc tgggaatgga aagcggaatc
     4981 tggcggcacc attacccgtc agatgcgccg tctcggcaac tccgtcgact gggagcgtga
     5041 acgcttcacc atggacgaag gcctgtccaa tgcggtgaaa gaagttttcg ttcgtctgta
     5101 taaagaagac ctgatttacc gtggcaagcg cctggtaaac tgggacccga aactgcgtac
     5161 cgctatttct gacctggaag tggaaaaccg cgaatcgaaa ggttcgatgt ggcacatccg
     5221 ctatccgctg gctgacggcg cgaaaaccgc agacggtaaa gattatctgg tggttgcgac
     5281 cactcgcccg gaaaccctgc tgggcgatac cggtgtagct gttaacccgg aagatccgcg
     5341 ttacaaagat ctgattggca aatatgtcat tctgccgctg gttaaccgtc gtattccgat
     5401 cgttggcgac gaacacgccg acatggaaaa aggcaccggc tgcgtgaaga tcacaccggc
     5461 gcacgacttt aacgactatg aagtcggtaa acgtcacgcc ctgccgatga tcaacatcct
     5521 gacctttgac ggtgatatcc gtgaaagcgc ccaggtgttc gataccaaag gtaacgaatc
     5581 tgacgtttat tccagcgaga tcccggcaga gttccagaaa ctcgagcgtt ttgctgcacg
     5641 taaagcagtc gttgctgcgg ttgatgcact cggcctgctg gaagaaatta aaccgcacga
     5701 cctgaccgtt ccttacggcg accgtggcgg cgtggttatc gaaccgatgc tgaccgacca
     5761 gtggtacgtg cgtgccgatg tactggcgaa accggcagtt gaagcggttg agaacggcga
     5821 cattcagttc gtaccgaagc agtacgaaaa catgtacttc tcctggatgc gcgatattca
     5881 ggactggtgt atctctcgtc agctgtggtg gggtcaccgt atcccggcat ggtatgacga
     5941 agcgggtaat gtttatgttg gccgcaacga agaagaagtg cgcaaggaaa ataacctcgg
     6001 tgccaacgtt gccctgcgtc aggacgaaga cgttctcgac acctggttct cttctgcact
     6061 gtggaccttc tctacccttg gctggccgga aaataccgac gccctgcgtc agttccaccc
     6121 aaccagcgtg atggtatctg gtttcgacat catcttcttc tggattgccc gcatgatcat
     6181 gatgaccatg cacttcatca aagatgaaaa tggcaaaccg caggtgccgt tccacactgt
     6241 ttacatgacc ggtctgattc gtgacgacga aggtcagaag atgtccaaat ccaagggtaa
     6301 cgttattgac ccgctggata tggttgacgg tatttcgctg ccagaactgc tggaaaaacg
     6361 caccggcaat atgatgcagc cgcagctggc ggacaaaatc cgtaagcgca ccgagaaaca
     6421 gttcccgaac ggcattgagc cgcacggcac cgatgccctg cgcttcaccc tggcggcgct
     6481 ggcgtctacc ggtcgtgaca tcaactggga tatgaagcgt ctggaaggct accgtaactt
     6541 ctgtaacaag ctgtggaacg ccagccgctt tgtgctgatg aacacagaag gtcaggattg
     6601 cggcttcaac ggcggcgaaa tgacgctatc gctggcggac cgctggattc tggcggagtt
     6661 caaccagact atcaaagcgt accgcgaagc gctggacagc ttccgcttcg atatcgccgc
     6721 aggcattttg tatgagttca cctggaacca gttctgcgac tggtatctgg agctgaccaa
     6781 gccggtaatg aacggtggaa cagaagccga actgcgcggt actcgccata cgctggtgac
     6841 tgtactggaa ggtctgctgc gcctcgcgca tccgatcatt ccgttcatca ccgaaaccat
     6901 ctggcagcgt gtgaaagtac tttgcggtat cactgccgac accatcatgc tgcagccgtt
     6961 cccgcagtat gatgcatctc aggttgatga agccgcactg gccgacaccg agtggctgaa
     7021 acaggcgatc gttgctgtac gtaacatccg tgcagaaatg aacatcgcgc cgggcaaacc
     7081 gctggagctg ctgctgcgtg gttgcagcgc ggatgcagaa cgtcgcgtaa atgaaaaccg
     7141 tggcttcctg caaacgctgg cgcgtctgga aagcatcacc gtgctgcctg ccgatgacaa
     7201 aggtccggtt tccgttacca agatcatcga cggcgcagag ctgctgatcc cgatggctgg
     7261 cctcatcaac aaagaagatg agctggcgcg cctggcgaaa gaagtggcga agatcgaagg
     7321 tgaaatcagc cgcatcgaga acaagctggc aaatgaaggc tttgtcgccc gcgcaccgga
     7381 agcggttatc gcgaaagagc gtgagaagct ggaaggttat gcagaagcga aagccaagct
     7441 gattgaacag caggctgtta tcgccgcact gtaatcaaca cagtgatgaa aacgaaggcc
     7501 ggagcatgct ccggcctttt ttatctctta cagaaatggt aaagaaggca taactccccg
     7561 agaaatactg gctaagaagc ctttatctgg ttgttcttct ttattgatta aaggaaggtc
     7621 atccaaatcg cccagcaaat acgtattttt tgcctgccag tcaatcatcc atattttcag
     7681 cagtgggtaa gccagaccac ccgtaatccc ggatatcacc accaacgcac acatgcgata
     7741 aagcataccg tggtacgtta aagttgagcg aaaacgaata cgcccatcat tcagtgacag
     7801 gttgctcata aaatgatttc gcaacgacac cgttaaataa ctggtgctaa cagcaatccc
     7861 aaaataataa attaactgcg cgattatcat tttacgttgc atttccataa attgctgtaa
     7921 attctcaata tcatcatttg catacccaga actatcatac tcatttaata tttgatcgaa
     7981 gataatataa ccagcaacag cgagaaaagg taataaagct aaaaatgcga ggatggcata
     8041 ttttatacaa tacgcagtat ctaatttcac cttgaaacga tgtataccga aactggtatt
     8101 gctccagaga aaactcatta ccaggctata taaagtacca ttaaaaatac caatggaaac
     8161 aataccaact attgccatca gaaccacaga tataataaca ctacttgaac tattggcatg
     8221 tagcatcttt gtcgagataa agaaaacagt ccccatccca atggccatta aaatcggcaa
     8281 gaaaaaggtt acccaccaga accctttcaa agagcaatta aaactaaatc ttacgccgtt
     8341 gagactggtc attaaggcct gataacgcag tccttttgct gccataaata taagcaaaac
     8401 caacagtaac aaagtcaaaa cacagccaac aagcggcata tctgctgaca ctgtcattaa
     8461 gattgcgaaa tagaaaaaaa caaaaacaag acaactaaca aaaacattcc caccggtaat
     8521 tccataagaa aatcgttgtc cgttaacttc catattagca taaagataac gcttacattt
     8581 cattaatgcc catggtaaat agatacctag cgttataatc gttaacaaca cattcaccac
     8641 acaaataaga aaatatctct cacctgtacc atgaaaaaca aaagaatggg acggaacatc
     8701 catttcatta ataacttgag ccataaaaat cccctgaatt aaaaaatcca ttgatataat
     8761 ttatagatac agacagtaaa atgttctgcc acgttacaat actcttatca gtaataggct
     8821 ggcaaaaact ttattgcttt ataagacgct tgcgcacgta agaagagagt ggtattaaac
     8881 aaatgtataa ttactttatt tttattgggt taaactatga ataacattgc gccgcaatca
     8941 cctgtaatgc gtcgcctgac gctgcaggat aatcctgcta tcgccagcgt catccgtcag
     9001 gtttacgcag agtatggtct taccgccgat aaaggctaca ccgttgccga tccgaatctc
     9061 gacgaactgt atcaagtgta cagccagcct ggccatgctt attgggtggt tgaatacaat
     9121 ggcgaagtgg tcggcggcgg cgggattgcg ccattaacag gtagtgaagc ggacatttgc
     9181 gaactgcaga aaatgtactt tcttcccgct attcgcggta aagggctagc gaaaaaactg
     9241 gccttaatgg cgatggagca ggcacgagag atgggcttca aacgctgcta tctggaaacg
     9301 accgcttttt taaaggaagc cattgcgctt tatgagcatt tgggctttga gcatatcgac
     9361 tatgcgcttg gctgcacggg ccatgtcgat tgtgaagtgc ggatgctacg cgaactctaa
     9421 aaaaatgccc gctagtatgt gctatcgggc attttaatca attaattagt ggcgaacccc
     9481 gtcatcgtct tcatcgacaa aatcttcatc gtcgccatct tcgccgttag gatcttcaaa
     9541 gtaagtgccc caaccgtcgt actcaacgtc aaatttctcc gccagcgtca tcagttgttc
     9601 aacctgggca tcgatcagat cggcattcaa cgcgcactcg ctgaggatgt cgcagcaaat
     9661 cacgatatca ccatcttcaa cttccagctc ttctggatcg gtcacttcgt aaccgagttt
     9721 aaacgcttca actgccgctt tttccagagt ttccagatcg tctgcggaaa gatggtgttc
     9781 gatggtgtac agcgcgtccg gatcgctgcc atcttccagt aattcttcaa taatcaaacg
     9841 tgtttcttca cgctgttctt ccagttgttc cgggtttgcc atggctcatt cctcttaatg
     9901 tcctgccgat actcttattg tcacacacca ctgacattgc ctccaccttt gtgacaaaga
     9961 tttatgcttt agacttgcaa atgaataata atacacataa attgagtttt aattcattga
    10021 ggcgttagcc acaggaggga tctatgtccg ggttttatca taagcatttc ctgaaattac
    10081 tcgatttcac gccagctgaa ctcaacagcc tgctgcagtt agccgcgaag ctgaaagctg
    10141 ataagaaaag cggtaaagaa gaagccaaac tcactggtaa aaacatcgcg ctcatcttcg
    10201 aaaaagactc gactcgtacc cgatgctctt tcgaagttgc cgcatatgac cagggggctc
    10261 gcgttactta tcttggccca agcggtagtc agattggtca taaagagtcg attaaagaca
    10321 ctgcccgcgt gcttggtcgc atgtatgacg gtattcagta tcgcggctat ggtcaggaga
    10381 ttgtcgaaac actggcggaa tacgctggcg tgccggtatg gaacggcctg accaatgagt
    10441 ttcaccccac gcagttgctg gcggatctcc tcaccatgca ggagcattta cccggtaaag
    10501 cattcaacga aatgacgctg gtctatgcag gtgacgcacg taacaacatg ggcaattcga
    10561 tgctcgaagc tgcggcgctt accggtctgg atttgcgtct ggtcgcgcca caggcatgct
    10621 ggccagaagc tgcgctggtt acggaatgcc gcgccctggc acaacaaaat ggcgggaata
    10681 ttacgctgac ggaagatgta gctaaaggag ttgaaggtgc tgactttatc tataccgatg
    10741 tatgggtgtc gatgggtgaa gcaaaagaaa aatgggctga acgcattgca ttactgcgtg
    10801 attatcaggt gaacagcaag atgatgcagt tgaccggtaa cccggaggtc aaattcctcc
    10861 attgcctgcc cgcgtttcat gacgaccaaa cgacgcttgg caaaaaaatg gcggaagagt
    10921 ttggcctaca tggcggaatg gaagtgactg atgaggtctt cgaatctgcc gccagcattg
    10981 tatttgatca ggcggaaaac cgcatgcata ccatcaaagc ggtgatggtc gcgacgctca
    11041 gtaaattaaa taattaatat ctctataaa
//
LOCUS       DADLTY010000141        10930 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.141, whole genome shotgun sequence.
ACCESSION   DADLTY010000141 DADLTY010000000
VERSION     DADLTY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.141"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..206
                     /locus_tag="JN334_003136"
     CDS             <1..206
                     /locus_tag="JN334_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439192.1"
                     /translation="FGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIA
                     IYLLAGCLISAMTALLMKDNQRS"
     gene            308..1762
                     /gene="amn"
                     /locus_tag="JN334_003137"
     CDS             308..1762
                     /gene="amn"
                     /locus_tag="JN334_003137"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY7439193.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2105..2821
                     /locus_tag="JN334_003138"
     CDS             2105..2821
                     /locus_tag="JN334_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7439194.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3277..3352)
                     /locus_tag="JN334_003139"
     tRNA            complement(3277..3352)
                     /locus_tag="JN334_003139"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3317..3319),aa:Asn,seq:gtt)
     gene            complement(3453..5080)
                     /gene="yeeO"
                     /locus_tag="JN334_003140"
                     /pseudo
     CDS             complement(3453..5080)
                     /gene="yeeO"
                     /locus_tag="JN334_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5085..5160
                     /locus_tag="JN334_003141"
     tRNA            5085..5160
                     /locus_tag="JN334_003141"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5118..5120,aa:Asn,seq:gtt)
     gene            complement(5198..6148)
                     /gene="cbl"
                     /locus_tag="JN334_003142"
     CDS             complement(5198..6148)
                     /gene="cbl"
                     /locus_tag="JN334_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HAY7439195.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6250..7167)
                     /gene="nac"
                     /locus_tag="JN334_003143"
     CDS             complement(6250..7167)
                     /gene="nac"
                     /locus_tag="JN334_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HAY7439196.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7495..7570
                     /locus_tag="JN334_003144"
     tRNA            7495..7570
                     /locus_tag="JN334_003144"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7528..7530,aa:Asn,seq:gtt)
     gene            complement(7626..8561)
                     /gene="ldtA"
                     /locus_tag="JN334_003145"
     CDS             complement(7626..8561)
                     /gene="ldtA"
                     /locus_tag="JN334_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7439197.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8623..9702)
                     /gene="cobT"
                     /locus_tag="JN334_003146"
     CDS             complement(8623..9702)
                     /gene="cobT"
                     /locus_tag="JN334_003146"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAY7439198.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9714..10457)
                     /gene="cobS"
                     /locus_tag="JN334_003147"
     CDS             complement(9714..10457)
                     /gene="cobS"
                     /locus_tag="JN334_003147"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAY7439199.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10454..>10930)
                     /gene="cobU"
                     /locus_tag="JN334_003148"
     CDS             complement(10454..>10930)
                     /gene="cobU"
                     /locus_tag="JN334_003148"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAY7439200.1"
                     /translation="SSQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDEL
                     IHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQR
                     CPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3037 a         2778 c         2367 g         2748 t
ORIGIN      
        1 tgtttggtgc cagttatcgc tacagtggtg ctggagtcgg ttatcaggtt gccagtgtgg
       61 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      121 ggcatagcat cgccatttat ttgctggctg gatgcctgat ttctgcaatg accgctttat
      181 tgatgaaaga caatcaacgc tcgtgatagc ctggcgaaga tcatccgatc ttcgccttat
      241 acttttgttt cacattcctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      301 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      361 cgcgctgtat gagcaatctg tagttgcttt acgcaacgcc attggcaaat atattacaat
      421 tggcgaatta cctgatgaaa acgcccgtaa acaaggtctt tttgtctatc catcactgac
      481 cgtaacctgg gacggtagca caacaaatcc ccccaaaacg cgcgcatttg gtcgttttac
      541 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatctgaa
      601 tgagcaacta acgttgcttt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      661 acatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      721 gagcgctgat ttaactcgct acttcccgac aacagaactg gcgcaaatcg gcgatgaaac
      781 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      841 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagtag aacattttca
      901 accgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
      961 cagccagatc ctcgatcctg atagtcccta cattgccctt tcttgtgctg gcgggaactg
     1021 gatcactgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1081 ccagatgcca gcatggcatt taattaccgc tgatggtcag ggtattactc tggtgaatat
     1141 tggcgtggga ccgtccaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1201 tatctggttg atgattggtc actgtggcgg attacgtgaa agccaggcca ttggagatta
     1261 tgtccttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1321 tatacctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcta ccaagttagt
     1381 cagcggcagg cctggtgagg aagtcaaaca gcggctgcgt acaggtactg tggtaaccac
     1441 agatgacagg aactgggagt tacgttactc agcttctgca cttcgtttta acttaagccg
     1501 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1561 gccatacggg acactactgt gtgtttcaga taaaccgtta catggtgaga ttaagcttcc
     1621 cggccaggct aaccgttttt atgaaggcgc tatttccgaa cacctgcaaa ttggcattcg
     1681 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcacgtaaat tacgaacctt
     1741 taatgagccg ccgttccgat aataagttat gaaccttctt atcagaaata gatgtgaagg
     1801 attaagtaag acatctggca aatagcctgc aataaacgcg ggctgcttgc ttccttgggc
     1861 agatgcgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca
     1921 aaacacaatt ataaaaaatc ccgccaacaa cattagtttt tataaaatta aatataagat
     1981 tatggcttta gaatattttt atttctaata gacgagattt ttcctgttat gatataatat
     2041 gccgaattaa cacatgttaa cgatttacca gtaatgtaaa taaattttcg aggagatcat
     2101 tccagtggga cgtaaatggg ccaatattgt tgctaaaaaa acggctaaag acggtgcaac
     2161 gtctaaaatt tatgcaaaat tcggtgtaga aatctatgct gctgccaaac aaggtgaacc
     2221 cgatccagaa ttaaacacat ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt
     2281 tccaaagcac gttattgata aagcaattga taaagccaaa ggcggcggag atgaaacgtt
     2341 cgtacaggga cgttatgaag gctttggtcc taatggctca atgattattg ctgagacatt
     2401 gacttcaaat gttaaccgta cgattgctaa cgttcgcaca attttcaata aaaaaggcgg
     2461 caatatcgga gcggcaggtt ctgtcagcta tatgtttgac aatacgggtg tgattgtatt
     2521 taaaggaaca gaccctgacc atatttttga aattttgctt gaagctgaag ttgatgttcg
     2581 tgatgtaact gaagaagaag gtaatattgt tatttatact gaacctgctg accttcataa
     2641 aggaatcgcg gcgctaaaag cagctggaat cactgagttc tcaacaacag aattagaaat
     2701 gattgctcaa tctgaagttg agctttcccc agaagattta gaaatctttg aagggcttgt
     2761 tgatgccctt gaagatgacg acgatgtaca aaaagtttat cataacgtcg caaatctcta
     2821 attatatttt aaagaaatct gtctttatgg cagatttctt taatctcata taattcttat
     2881 aaaaaatata atatttaact cgtcatattg cttataaaaa atccccgtcc ccagagaaat
     2941 aatatttatt aaaatggcag gtcttctttt tctgattacc gaagacaaag tggcaattac
     3001 gtatagtttc ccgataaaga cgcgacagct acgtcccgca taaggcattt ttctctttat
     3061 ctttgtacgg tacttcatgg aacagaattt ttgaccttgt gaatcgtgat atctgttggg
     3121 gaggggcaat ttgctcactg aagcgtgaga ctcgattaag cgcacgaaac acagaaatca
     3181 aaaaacccgg ccactttttt acaaagtggc cgggtaaaaa taatttttat tttttaactg
     3241 tcctgagtct catagagatg tctcaaaact aaaatttggc tcctctgact ggactcgaac
     3301 cagtgacata cggattaaca gtccgccgtt ctaccgactg aactacagag gaatcgtgag
     3361 aacgaggcga atattagcga tgcccaccca caatgtcaaa gcctgttttt taaatttgaa
     3421 atcgtttgct gaaataatct gcattttgtc gtttattccg acacaactgg ctttttttca
     3481 cacttttgcg gctcggatcg agggtatttc cacagccaac gtccagtaac cattcgccag
     3541 taaaacagca cggcccgcac agcccagtcg gcaaacattc ccatccagac accaaccaca
     3601 ccccagccaa gcatgattcc ctgcacataa ccgactacaa cccggcaacc ccacatgctc
     3661 aacatagaaa cccacatggc gtaacgggca tcacgagcac ccttaaatcc agcgggtagc
     3721 acccatgagg cggaccaaat aggcataaat aaagcattta gctaaatcag aatcacaacg
     3781 acgtgtttaa cctgtggatc ctgggtgtaa aacgatgcca taaccccggc aaagggagcc
     3841 gttagccagg cgatggccgt taatccaaga gtggacagcc agaacacatg ccgcaactga
     3901 atctctgctt gcgctatctg ccctaccccc aaccttcggc ctgtaatgat cgtagaagca
     3961 gaaccgagcg cacttccggg taagttgata agagccgcaa ttgagaacgc gataaaattt
     4021 ccggcaataa cgctggtccc catcccggca acgaacattt gggttaataa ccgaccactg
     4081 gtaaataaca ctgattcgac actcgctgga ataccaatcc ccatgacttc ccagataatg
     4141 ctaaaattca gcggtttaaa atagctcttt aacgaaatcc ttagcgcagg attaaaacca
     4201 atcgccagca cccacaaaat tgcaactgcg ccaatataac gagaaatggt taaacccaac
     4261 ccagccccga ccaggagaaa aggccgtaaa tcaatatgcc gctaataata atattaagaa
     4321 tattcaggct accgttaatc agtagcggta ttttcgtatt ccctgcacca cgaagtgccc
     4381 cgctaccaat aagagtgatg gcagcggctg gataactgag tacggtcagc tccagataag
     4441 tcaacgccag tgctttaact tctgtcgtgg catcacccgc gacgaaatca ataatttgtt
     4501 cgccaaaatg atgaattagc gttgccaaca gtacggcaaa caacgtcatg atcaccaatg
     4561 actgccgcgt cgccaccctc gctcgtcgtc gatcccgctt accgagacta aatgctacaa
     4621 cgacagtagt accaagatcg atagctgcaa aaaaagccat aatgaccata ttgaagctgt
     4681 ccgccaaccc cacgccggcc atcgcatctt ttcccagcca actgaccaga aaagtgctca
     4741 ggacccccat caacaggaca caggcgttct ccatgaagat aggaacagca agcggggtta
     4801 tctcgcgcta gaacaacact ttgtagctct tgcgtttaga gtgccagcga gtgccgtgaa
     4861 caacctggcg taaagcagag gagatattca aagccgacct taattgcaga aagtgaaacc
     4921 acatttcaaa tgatgaggga gaatcagcaa agctgcaaag attttcgcca acaaattgtc
     4981 tgcaaatgca acaaactgtt gatagaaacg gcaaacagtt gggaaattta aaaatcgggt
     5041 ttgacaaaat atttttcgcc gttaagatgt gcctcaacag cgattcctct gtagttcagt
     5101 cggtagaacg gcggactgtt aatccgtatg tcactggttc gagtccagtc agaggagcca
     5161 aatttctgaa aagcccgctt ttatagcggg ctttttgcta tatctgataa tcaatttcct
     5221 cttcactgtt ttccatcacc tggcgcttga tatcctcaac tgacagtcct gcattacaaa
     5281 gttccagaaa gcgccaaaca tagttacgct gaagttgtcc tcgcttcagt cccaaccaga
     5341 cagtattagc atcgaaaaga tgccgcgtat ccaggcggat taaattctct tcctcttgtt
     5401 cgccgctgga ttgttcggca actaatccga tcccaagccc aagagcaaca taggttttaa
     5461 tgacatcaga atcctgtgca cttaatacaa tatctgccgg caaacctttg cgggtaaatg
     5521 cgtcatcagt gcgtgagcgc cccgtaattc cctgtcggta agtaattaac ggccacttcg
     5581 ctattgattc cagcgtcaat ggtgtaattt gcgttaaggg atgatcgagt ggaacaagca
     5641 aactatggtg ccaacaaaac catgggaagg cgacgagctg cgggtcatta ctcaaacgct
     5701 cgctggcgat accaatatca gcttcgccat tttgcaacaa cgtcgcaatt tcctgtggcg
     5761 aaccctggat tagctcgagc cgaacctccg ggaaaagttc gcgaaaagct ttaatgacct
     5821 ctggcaagct ataacgtgcc tgagtatgcg tcgttgcaat agtgagaaca ccagacgtat
     5881 cgttggtaaa caggtctgca agccgacgaa cattactggc ttcattcaga atacgttctg
     5941 caatgaccag taatgctttg cccggttcag tcatccccag cagtcgctta cctcgtcgaa
     6001 caaatatttc gatgccaaat tcatcctcca gttcccgaat atgacggcta acgcctgact
     6061 gtgaggtaaa aagcatattc gcaacctctg tcaggttgta atcctgacgt gcagcctcgc
     6121 ggattatctt tagttgttgg aaattcacgg taaactccgg gcagttcaga tatcccgtta
     6181 ttgttaaagt ctaatgcccg gcataacaaa taataaaaac ccgcatctta ttccatcccg
     6241 atataacgct tagctcacca attgccactg ccttttttcc atcactggag aactaatcac
     6301 tgacattaac aactctttca ctgcctgtgc ctgtggtgac aagtttgctc tggcgggtaa
     6361 atttaaggac aaagagagac tcattgaagg agtggtaatg cgtgacatcc acccatttac
     6421 tgcgccacat aacgaacgcg cagccgattc aggtaatact gcaacgccca tgccgctggc
     6481 aatcgctgcg gtaagcgtgg caatagactc aatttcacca atgacttttg ccgtgagccg
     6541 ccgtagggaa aaagcctcat caacacgaag tctaacagcg ctgtaatcac tggggagaaa
     6601 aaggttcatt tgcgcaatag cattaacatc aacgttttgc cccgggcaat cttgagttcc
     6661 taccaggaaa agatcttctt tcagcaaagc ctgactggat accccagcca caggggaatg
     6721 ctcataaatc accgccatat cgagttggtg atttatcagt ttttcgttaa gcactgcacc
     6781 actattttca tgaagataga taacgacctc cgggaattca gcgcgaaccg cctgtaataa
     6841 gggcatggtg atggatgacg cagcggttcc tggcgcaaag ccaatcgaga cttgccccga
     6901 taatgactga ccaacgttat gcaccgccag ttgggcctgc tcacactgac gtaaaatggc
     6961 ccgtgcatgg gtatagagaa tttttccggc gtctgttggc gtaacgcccc gttttgtgcg
     7021 aatcaaaagc tgttgattta actcaccttc cagtatggca acctgttggc tgagcgctgg
     7081 ttgtgcgata tgcaatactt cagcagcctg ggtcaggcta ccaatatcta caatttttac
     7141 gaagtatttc aggcgtctga agttcatgtt gcctccggtt tttaagaatc ggcccaagtg
     7201 ccgccattac ttacaaccag tatgcaagat gcctgccagt tctattttgg cgttgatgcg
     7261 caagctaacc aactgtcaaa taagagatta tgatagattc gtcattggcc ctttttatca
     7321 gcagtcgcat tcacctgccc tataaaagga gggtatgcac cacgatggtt cattacccaa
     7381 taagattgaa agctcaccac tttgttgaaa ttggcagcaa acaaacaaaa aaaatgcatt
     7441 tcaccctttg acatcaccat gcactgccat taatatgcgc cccgttcaca cgattcctct
     7501 gtagttcagt cggtagaacg gcggactgtt aatccgtatg tcactggttc gagtccagtc
     7561 agaggagcca aattcaaaaa agcctgcttt cgagcaggct ttttactttc taattaccaa
     7621 cgctcttaaa acatctgtct tgaaccagaa ctaatttgca caggcattcc cgatcgatgt
     7681 tgcaacgcag catttgcgcg atttacatca acttcttgcc cgttgataaa cgcccgcaaa
     7741 gatggggtta ccggcaatgg cacttttcgg tcagactcat attctgcacg attgcgcgac
     7801 agcggctcat gaacttccag ccactttgaa ccatccggtt ccgttgtgta tttcactggc
     7861 tgatcaataa tttgtacacg agtgcctaca ggaacattat caaacagata tttgatatcg
     7921 tcattacgca gacgaataca gccctgactt acacggagcc caataccaaa attggcattg
     7981 gtaccgtgga tggcatacaa cctgccaata taaatcgcgt acagccccat ggggttatcg
     8041 ggccccgcag gaacaaatgc gggcaaactc tcccctcgtt tcgcatattc gcgccggatg
     8101 ttcggcgttg gcgtccaggt tggtgcttct tgtttacgtt caacggtagt cacccagtta
     8161 cgcggggttt ctcgcccagc ctggccgata ccaataggaa agacttccac tgtattactg
     8221 tctggcggat agtaataaag acgcatttca gcgacgttaa ccacaatccc tttacgaaca
     8281 gtggcgggca aaatcagttg ctgcggaatg gtgagttgtg agccaggctt cggcaaaaaa
     8341 acatcagcgc ccggattcgc ttccagcata ttacttaacc cttgcccgta ttgtgcggca
     8401 aaagtctcca gcggctgagt attgtgatca ggaacagtta cagtaagcga ctgccccact
     8461 aaacggctac cctctggagg taatggataa gttaccgcca ggctattttg gctggcaaaa
     8521 agcagagcaa atgagcaaag aatatttaca cgacgcatca tgtccctttc ctaagtcgcg
     8581 aatactatcc gataagtata gcttttatca gacttttcgt ttttagctgt tcaaatcaga
     8641 agtcgtattc cccggtagaa caatattact ggcagcaagt tcgcccatgt tgttgtatat
     8701 cgcacaagca gcttcgatga tggacatcgc cagagctgca ccactcccct cacctaaacg
     8761 catatccata ttgagataag gctccagccc caaatgcgag agcgctatac gcgcgccttt
     8821 ttctgccgac aagtgagaag gaatgagata cggtttgatt gcaggagaca tctggcaggc
     8881 tgcgagcgca gcagcataag aaagaaatcc atccagcaac acgggtaaac cgcaggaagc
     8941 agcacctaac atcactccag ctattccgac caaatcaaat ccacccactt ttgccaggac
     9001 atcaacacca tcctgaggat ttggttgatt caacgtaatc gcccgacgca caacatcaat
     9061 tttattagcc agtttatctg tcggcaggtt tgcgccaatc ccaaccactt cttcaggatc
     9121 ccggccagtg attgtgctga ctattgccgc tgccggtgtc gtgtttgcca tccccagttc
     9181 acctacacca aacagcgtga caccgttttt cgccagctcc cgcgtataac atatgacgtc
     9241 caaaagcaac ttttcagcct gacggcgact cattgccgga gctgaagcaa tattgccgct
     9301 acctcgtgcg acacgcatgt tgataagccc ggggataggc tcagcagtat caataccaac
     9361 atcaattacg tggacgttag cgcccgcttg tgctgccagc acacacacgc cggttgttcc
     9421 acgggtcata ttttcagcct gtatggctgt cacttctttt ggagaaatag cgaccccttc
     9481 ctcccagacg ccgtgatcgg cacacataac cagtaccgct tttttgccca catgcggtat
     9541 gccattcacc cccggcattc ctgccagttg tatggcaagc gcctccagct ttcctaggct
     9601 accaacaggt ttgaataacc cgtcaacatg ccgttgtgca cgcgacatag cggcagaatt
     9661 gatggctggg atcgtattca gtaaatcggc aattatttgc atgtcacgtt tctttatagc
     9721 agtgccagca ggaagaccag ttcaccaagc tctatggctg ctcccagcgt atcgcccgtt
     9781 tgcccgccca gcgtacgttt gagaagctga ccgaggatga aaatcgccac catcgtcacc
     9841 accatagcag ccacaccatg catacccagc aataatacgg cagcaaaaat agcggctaag
     9901 ccgagtgtga cgcaggtttg tcgcccatca attttgccaa tgaacacatt gccaagccct
     9961 tcttcccgcg cataacgatg acgatacatc aataatgcgg cagtcccgcg gctgaccgca
    10021 catgctgccg ccagcgatgc aaggatcggt tcgccacgca gagccaactc gcttaacacc
    10081 agaatctttg ccagtaccac aaaaatcaat gccagaccgc cgtgggtgcc taaacggcta
    10141 tcacgcatga tttccaacat gcgatcgcga ctacgtgcag aaaatacgcc atcacaggta
    10201 tcagcgaggc cgtccagatg gaattccccg gtcatcagcg ccagcacaag tacgctaaac
    10261 agtgccgcca gtggtgcgcc acaccatgcc tgcagcacca tgaagaccag cccgctaatc
    10321 gcgccaagca ataatccaat caaaggaaaa gtaataatac cgcgagaata atgttcgaaa
    10381 tccagtccct gggaccagcg acgcggtaca ggcagacgcg taataaatga gagcatcgcc
    10441 caaaataatt tgctcatttg atttttactc caatacccga aaccaccagc catacttcat
    10501 ttgccgcagc ggccaactgc tgatttaccc gcccggcaat atcacgaaaa tgtcgtgcca
    10561 gacgactctc cggcacaatc cccattccca cttcgttagt cactaataca acctttgcgg
    10621 ggcaacgttg gcaggcagca atcaacgact gaatctcagc attaatcgcc tgttccatcg
    10681 cctgataatc ccattcatca gggtctttat cgccgccata atcaaacaac agattagtca
    10741 ccattgtggt aacgcattcc agcaacacag cctcattcgg gttaatgtct gcatgaatta
    10801 attcatcaag atgttgccag cgctccactg tgcgccagtg ctccgggcgg ctttgccgat
    10861 gatgttctat ccgtgcagcc atctcatcat caaggatttg cgaggtagcg atatacagaa
    10921 cctgtgaaga
//
LOCUS       DADLTY010000142        10926 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.142, whole genome shotgun sequence.
ACCESSION   DADLTY010000142 DADLTY010000000
VERSION     DADLTY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.142"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            154..504
                     /locus_tag="JN334_003149"
     CDS             154..504
                     /locus_tag="JN334_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAY7439201.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            557..952
                     /locus_tag="JN334_003150"
     CDS             557..952
                     /locus_tag="JN334_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439202.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="JN334_003151"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="JN334_003151"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HAY7439203.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="JN334_003152"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="JN334_003152"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HAY7439204.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /locus_tag="JN334_003153"
     CDS             complement(2869..5298)
                     /locus_tag="JN334_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY7439205.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="JN334_003154"
     CDS             complement(5323..6423)
                     /locus_tag="JN334_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAY7439206.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="JN334_003155"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="JN334_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAY7439207.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="JN334_003156"
     CDS             complement(7571..8137)
                     /locus_tag="JN334_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY7439208.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="JN334_003157"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="JN334_003157"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAY7439209.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10263..10751
                     /locus_tag="JN334_003158"
     CDS             10263..10751
                     /locus_tag="JN334_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HAY7439210.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2669 a         2789 c         2605 g         2863 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattccc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgtgtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtga cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata
    10681 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    10741 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    10801 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    10861 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
    10921 aaccgc
//
LOCUS       DADLTY010000143        10556 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.143, whole genome shotgun sequence.
ACCESSION   DADLTY010000143 DADLTY010000000
VERSION     DADLTY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.143"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            38..778
                     /locus_tag="JN334_003159"
     CDS             38..778
                     /locus_tag="JN334_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7439211.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            837..1223
                     /locus_tag="JN334_003160"
     CDS             837..1223
                     /locus_tag="JN334_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HAY7439212.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1232..1561
                     /locus_tag="JN334_003161"
     CDS             1232..1561
                     /locus_tag="JN334_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HAY7439213.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1533..4574
                     /locus_tag="JN334_003162"
     CDS             1533..4574
                     /locus_tag="JN334_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7439214.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4574..4903
                     /locus_tag="JN334_003163"
     CDS             4574..4903
                     /locus_tag="JN334_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7439215.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            4903..5601
                     /locus_tag="JN334_003164"
     CDS             4903..5601
                     /locus_tag="JN334_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAY7439216.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5606..6349
                     /locus_tag="JN334_003165"
     CDS             5606..6349
                     /locus_tag="JN334_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7439217.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6286..6888
                     /locus_tag="JN334_003166"
     CDS             6286..6888
                     /locus_tag="JN334_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HAY7439218.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            6949..10428
                     /locus_tag="JN334_003167"
     CDS             6949..10428
                     /locus_tag="JN334_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAY7439219.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10496..>10556
                     /locus_tag="JN334_003168"
     CDS             10496..>10556
                     /locus_tag="JN334_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HAY7439220.1"
                     /translation="MRKLYAAILSAAICLAVSGA"
BASE COUNT         2487 a         2498 c         3388 g         2183 t
ORIGIN      
        1 acgtaccaga tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc
       61 aaagacgaaa ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg atgcgtttgc
      121 gaaccctttg tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg
      181 cgaaatgacc gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa
      241 aacgacaacc caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc
      301 gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt
      361 tcgtatcaaa tatcccaacg gcactgttga tgttttccgc ggctggctga gctcactggg
      421 taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg
      481 tccgtatctg gcagaggaag gcactgaaac cgtgggtgtt accggactga cggtggcacc
      541 ggcatctgtc agtgtcaaag cgggagcaac caccacactg acctttacag taaaacctga
      601 cggggccagt gacaaagcga tcagtgtgca ttcgtcagat cctcagactg cttcggtgac
      661 cctgagcggg cttgtggcca cggtgaaagg cgtgaagcag ggcagtgtca gcatcgtggg
      721 catgacctct gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca
      781 ggacgatact catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt
      841 ttctgaaaca ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg
      901 ggctgcagag aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgcac
      961 aggagggaga actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata
     1021 tcaatgcctg gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga
     1081 cgctttgcgc atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga
     1141 gggttctgtc gctgagcggt atggggacca ttgagaatgc cggggatgat gatcatgagg
     1201 cgctgacgcc ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga
     1261 gttccggcgg gcagactggc ggcggatgct gtcggatatg tcggccactg agcttggtga
     1321 gtggggcgat tatttccgga tgcagagctt cagtgatgtg tggatggatg cgcagtttgc
     1381 ctcgctgaag gcattgatcg tgagaatggt gtccggcagc agcgatgctg cggtggctga
     1441 tttcagcctt ttaccggaag agaacgggat accggagcga acggacgaag aactgatgca
     1501 tcttggggaa ggtatttccg gaggtgtgcg ttatggacca gatagccaac ctggtcattg
     1561 atttggggat tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa aaccttctga
     1621 atggtgcagc cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt atggagcgtc
     1681 agactcaggc ggcccggcag acaatgcagg cggcttcttc ggctgcaaca gcagcatcag
     1741 cccatgcgca gacggtggag aagaatgcac gggctcatga acgcatggcc cgtgaggtgg
     1801 aacaaacccg tctgcgcgtg gatgccctga atcagaaaat gcgcgaggaa caggcgcagg
     1861 ccagggcact ggcggaggcg caggataaag cggctgccgc cttttatcgc cagattgaca
     1921 gtgtgaaaca ggccggtgcg gggcttcagg aattacagcg tattcagcag cagatccgac
     1981 aggccagaaa cagtggcggg gttggtcagc aggattatct ggcgctgatt tcggagatca
     2041 cggcgaaaac ccgtgccctg acgcaggcag aggaacaggc cacccggcag aaagcggcgt
     2101 ttatccgcca gcttaaagag caggcaaccc gccagaatct gtcgtcttct gagttgcttc
     2161 gcgccagggc ggctcagctg ggggtaagca gtgctgcaga agtgtatatc cgcaaaatgg
     2221 agcgggcagg aaaagccaca cattcgctgg ggctgaaaag tgcggcagcc cggcgggagc
     2281 tgggggtgtt aatcagtcag atggcgcgag gcaattttgg tgcgctgagg ggatccggga
     2341 taacgctggc taaccgtgcc ggatggacag gcgcactgat gtcgccgaaa ggcatgatga
     2401 ctggcggcgt tattggcgga cttgtcgcgg cggtcctggg tctgggtaaa gcctggcatg
     2461 acggccggaa ggagggcgag aaatttaacc gtcagctggc gctgacggga cattatgccg
     2521 gtgtcactgt cgggcagttg tggaaactca gccgggccat atccgggaat ggtatcacgc
     2581 aacatgcggc agccggtgcg ctggcacagg tggtgggcag tggggcattt catggaaacg
     2641 atattggtat ggtggcgaaa gctgccgcac agatggagcg atcggttggc cagtcggtca
     2701 gcgataccat aaatcagttt aagcggctga aggatgatcc tgtaaatgcc gcgaaggcgc
     2761 tggacaatgc gctgcatttt ttgaatgcca ctcagcttga gcagatacgc gtccttgggg
     2821 agcaggggcg gtccagtgat gcggcccgga tcgccatgtc tgcgctggca gaggaaaccg
     2881 gtaaacgcac atctgatatt gataataatc tcaatgcgct gggtagtacg ctgcaaacct
     2941 tgtctgactg gtggaagcag ttctgggatg cggccatgaa tattggtcgt gaagactcgc
     3001 tggatgcgca gattgatgcg ttacaggaaa aaattcagcg cgcgaaaaaa tatccgtgga
     3061 caaacgcctc cacacaggtg gagtacgatc agcagcgtct taacgatctt caggagaaaa
     3121 aacgccggaa ggatttgcag gatgcaaaag cgcaggcaga acggaattac caggagcaac
     3181 agaaacgccg gaatgctgaa aatgccgcgc tgaaccggat gaatgaaacg gaagctgcac
     3241 gacatcagcg ggaaattgcg cgtattaatg ccatgcagta cgccgaccag gctgtcaggg
     3301 atgcggcgat acagcgtgaa aatgaacgtt acgaaaaagc cattaagaaa aatacacggg
     3361 caacccgtaa tgatgaggcc acccggttat tgctgcagta cagtcagcag caggcacagg
     3421 tggaagggca gattgctgct gccagacagt cagcaggcat tgccactgaa aggatgactg
     3481 aagcgcataa acagcttctg gccctgcagc agcgcatcag cgacctggac gggaaaaagc
     3541 tgacggcaga tgaaaagagt gtgctggccc gtaagaatga gctgattcag gcgctgacgc
     3601 tgctggatgt gaaacagcag gagctgcaga aacagacagc gcttaacgac ctgagaaaaa
     3661 aaacggttca gctgaccagc cagctggcag acaaagaacg tgcactgcgt gagcagcaca
     3721 atctggatat tgccactgca ggtatggggg ataagcagcg gcagcgctac caggcacagt
     3781 tgcgcatccg gcaggaatac cggcaacagt tgcaacagct tgagaatgac agtcgccaga
     3841 aaggcactta cgggacggag gactaccgga gggctgagga ggtgctgaag gggagcctga
     3901 agcgacaact gaatgaaaac aaacgctact ggcaggaact ggaagtggcg cagggcgact
     3961 ggaaaaacgg tgccatgcgg gcgtttcaga attttacggc ggatgcggat aatgcggcgg
     4021 gaacggcaga acagatgttt acagtggcat tcagcagtgc cggtaatgca ctggcgacat
     4081 tctgtaccac cggtaagctg aattttaaat ccttcacctc ttccctgttg tcagatatgg
     4141 ccagaattat ggcacagatg gccatgatgc aggcggtaaa gggcgtcggt tctttattcg
     4201 gcttcacgac taatgctgat ggcggtgttt accagtctgc tgatttgagt cgctacagtg
     4261 gcacggtggt taaccgtccg acgttttttg cttttgcaaa aggcgcgggt gtgatggggg
     4321 aagcgggacc tgaagccatt ctgccactgc gtcgcggtgc tgatggtaag ctgggggttg
     4381 tggcggatac tggtggttca ggcatggtga tgtttgcccc gcagtacaac attgagatca
     4441 ataacgacgg cacgaacggg cagataggtc cggctgccct gaaggtggtt tatgacctcg
     4501 ggaaaaaagc ggccgcggac tttatgcaac agcaggcccg tgatggtggc cggttaagtg
     4561 gagcataccg gtaatggaga cgtttcactg gaaagtgcgc ccggatatga atgtggtatc
     4621 agagccgaaa gtggtgacag tgaagctggg cgatggttat gaacagcgtc gtgcggcggg
     4681 actgaataac cagttgtcga cttacagcgt gacgatacgt gttcgtaaag gtgaacaccc
     4741 atctttaaaa gcctttctgg aacggcacgg tggcgtccgc gcatttcagt ggacgccacc
     4801 ttatgactgg aaactgatca gggtggtttg tcgtaaatgg tcggcaagcg tgggggcgtt
     4861 atgggtgact gtaacggccg attttgaaca ggtggtgaac tgatgcagga tatccggcag
     4921 gaaacactga atgaatgcac ccgtgcggag cagtcagccc gggtggagct ctgggaaatc
     4981 gatctgacag aggtcggtgg tgagcgttat tttttctgta atgagcagaa cgaaaaaggt
     5041 gagccggtca cctggcaggg gcggcagtat caggcatacc ccattcaggg gtcggggttt
     5101 gaactgaacg gcaggggctg tgctgcccgt ccgacactga cggtctctaa cctgcacggc
     5161 atggtcaccg ggatggcgga agatctgcag agtctggtcg gcggaacggt ggtccggcgt
     5221 aaggtttacg cccgttttct ggatgcggtg aacttcgtca acggaaacag cgacgccgat
     5281 ccggaacagg aggtgatcag ccgctggcgc atcgagcagt gcagcgaact gagcgcggtc
     5341 agtgcctctt ttgtgctggc cacaccaacg gagacggatg gcgcggtttt cccggggcgt
     5401 atcatgctgg cgaatacctg tatgtggact taccgttctg atgagtgtgg ttacacgggc
     5461 agggcagtgg ctgacgagtt cgacaaacca acaacggata tccggaagga caaatgcagc
     5521 aagtgtatgc gcgggtgtga gttgcgcaac aataccggta atttcggcgg tttcctttcc
     5581 atcaataaac tttctcagta aatccatgac acagacagaa tcagcgattc tggcgcacgc
     5641 ccggcgatgt gcgccagcgg agtcgtgtgg cttcgtggtg agaacgccgg agggagacag
     5701 gtatcttccc agcgagaata tctccggtga gccggaggaa cggttccgga tggctccgga
     5761 ggactggctg cgggcacaaa tgcagggtga gattgtggca ctggtccaca gtcatcccgg
     5821 tggtctgccc tggctgagtg aggctgaccg gcggctgcag gtgcagagtg atttgccgtg
     5881 gtggctggtc tgccggggga cgattcacaa gttccgctgt gtgccgcatc tcaccgggcg
     5941 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc
     6001 ggggattgag atgccggatt ttcatcgcgg ggatgactgg tggcgtcacg gtcagaatct
     6061 ctatctggat aatctggagg ccacagggct gtatcaggtg ccgttgtcat cagcacaacc
     6121 gggcgatgtg ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg
     6181 cggcgatggt gagctgctgc accatattcc tgaacaactg agtaaacgag agaggtatac
     6241 cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggaatggc acgcatctgc
     6301 ctttacgggg atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggggct
     6361 gaagccatcc gcgcactggc cacacagctc ccggcgtttc gtcagaaact gagtgacggc
     6421 tggtatcagg tgcgcattgc cgggcgtgat gcaggtgaaa ccgaattgtc tgcccgtctt
     6481 aatgagccgc tggcaaatgg tgcagtgatc cacatagtac cgcgtctggt gggagctaaa
     6541 agtggcggtg tgtttcaggc ggtgctgggg gcagctgtta tggcggttgc tatatggatg
     6601 ccgggggtag gaattatggc gagtaatctg ctgttttctc tcggtgccag tatgacgctt
     6661 ggcggtgttg cacagatgct ggcaccgaaa gccagaactc cccgtacaca gacaacggat
     6721 aacggcaaac agaacacctg tttctcctca ctggataaca tggttgccca gggcaatgtt
     6781 ctgcctgttc tgtacggtga aatgcgcgtg gggtcgcggg tggtatctca ggagatcagc
     6841 acggcagacg aaggggatgg tggtcaggtt gtggtgattg gtcgctgatg caaaatgttt
     6901 catgtgaaac cgcctgcggg cggttttgtc gtttatggag cgtgaggaat gggtaaaggc
     6961 agcagtaagg ggcatacccc gcgcgaagcg aaggacaacc tgaaatccac gcagttgctg
     7021 agtgtgattg atgccatcag cgaagggccg gttgacggtc cggtggatgg attaaaaagc
     7081 gtgctgctga acggtacgcc ggtcctggat agcgagggga agacaaactt ttccggtgtt
     7141 acggtggtgt tccgcgccgg cgagcaggag cagacaccgc cggaggggtt tgaatcttcc
     7201 ggctcagaga ctgtgctggg tacggaagtg aaatacgaca cgccgatcac ccggaccatc
     7261 acgtcggcaa acattgatcg tctgcgcctg accttcggtg tgcaggcact ggtggaaacc
     7321 acctcaaagg gggaccggaa tccgtcggaa gtccgcctgc tggttcagat acagcgtaac
     7381 ggtggctggg tgacggaaaa agacatcacc attaagggca aaaccacttc gcagtatctg
     7441 gcctcggtgg tggtgggtaa cctgccgccg cgcccgttta atatccggat gcgcaggatg
     7501 acaccggaca gcaccacaga ccagctgcag aacaaaacgc tctggtcgtc gtacaccgaa
     7561 atcatcgatg tgaaacagtg ctacccgaac acggcgctgg tcggcgtgca ggtggattca
     7621 gagcagttcg gtaaccagca ggtgagtcgc aattatcatc ttcgcgggcg cattctgcag
     7681 gtgccgtcga actataaccc gcagacgcgg caatacagcg gtatctggga cggaacgctt
     7741 aagccagcat acagcaacaa catggcctgg tgtctgtggg acatgctcac tcatccgcgc
     7801 tacggcatgg ggaaacgtct tggtgcggca gatgtggaca aatgggcgct gtatgtcatc
     7861 ggccagtact gcgaccagtc agtgccggac ggatttggcg gcacggagcc gcgcatcacc
     7921 tgtaacgctt acctgaccac acagcgtaag gcgtgggatg ttctcagtga tttctgctcg
     7981 gcgatgcgct gtatgccggt atggaacggg cagacgctga cgttcgtgca ggaccgaccg
     8041 tcggataaag tgtggaccta taaccgcagt aatgtggtga tgccggatga tggcgcgccg
     8101 ttccgctaca gcttcagcgc cctgaaggac cgccataatg tcgttgaggt gaactggatt
     8161 gatccggaca acggtcatga gacggcgaca gagcttgtgg aggacacgca ggccattgtc
     8221 cgttacggtc gtaacgtcac gaagatggat gcctttggct gtaccagccg gggacaggcg
     8281 caccgcgccg ggctgtggct gattaaaacg gaactgctgg aaacgcagac cgtggacttc
     8341 agcgtcggcg cagaagggct tcgccatgta ccgggtgatg tcattgaaat ctgcgatgat
     8401 gattatgccg gtatcagcac cggtggtcgc gtactggcgg tgaacagcca gacccggacg
     8461 ctgacgctcg accgtgaaat cacgctgccg tcctccggta ccacgctgat aagcctggtt
     8521 gacggaaatg gcaatccggt cagcgtggag gttcagtccg tcaccgacgg cgtgaaggtg
     8581 aaagtgagcc gggttcctga cggcgttgca gaatacagcg tgtgggggct gaagctgccg
     8641 acgctgcgcc agcgcctgtt ccgctgcgtg agtatccgtg agaacgacga cggcacgtat
     8701 gccatcaccg ccgtgcagca tgtgccggaa aaagaggcca tcgtggataa cggggcacac
     8761 tttgacggtg accggcgcgg cacggtgaat ggtgtcacgc cgccagcggt gcagcatctg
     8821 accgccgaag tcaccgcaga cagcggggaa tatcaggtgc tggcgcgatg ggacacgccg
     8881 aaggtggtga agggtgtgag cttcctgctt cgcctgaccg tggcagcgga cgacggcagt
     8941 gagcggctgg tcagcacagc caggacgacg gaaaccacat accgcttcag gcagctggcg
     9001 ctggggaatt acagtctgac agtccgggcg gtaaatgccc gggggcagca gggcgatccg
     9061 gcgtcggtat cgttccggat tgcggcaccg gcagcgcctg tcactattga actgataccg
     9121 gggtattttc agataacggt ggtcccgaaa ctggctgtat atgacccgac ggtgcagttt
     9181 gagttctggt tttcggaaaa gcggattgcg gatatcaggc aggttgaaac cagcgcccgc
     9241 tatcttggca cggcgctgta ctggatagcc gccagtatca atatcaggcc gggccatgat
     9301 tattattttt acgttcgcag tgtgaacacc gttggcaaat cggcatttgt ggaggccgtc
     9361 ggtcgggcga gcgatgatgc ggaaggttat ctgagttttt ataaagggtt gatcaataaa
     9421 acgcatctcg gcaaggagtt gtggacgcag attgataacg gtcagcttgc gccggacctg
     9481 actgaaatca ggacgtccat tacgaatgtc agcaatgaaa tcacgcaaac cgtcaataaa
     9541 aaactggaaa atcagagtgc tgcaatccag cagatacaga aagttcaggt tgatacaaat
     9601 aataacctga acagcatgtg ggctgtgaag ctgcaacaga tgaaggacgg acgcctttat
     9661 attgcgggta tcggtgccgg tattgagaat acgccagcag gaatgcagag tcaggtgctg
     9721 ctggcggcag acaggattgc gatgattaat cctgcgaatg gcaacacaaa gccgatgttt
     9781 gttggtcagg gcgatcagat attcatgaac gacgtgttcc tgaaacgcct gacggctccg
     9841 accattacca gcggcggtaa tcctccggca ttttccctga cacctggcgg acggctgacg
     9901 gcgaaaaatg ccgatatcag cggtaacgtg aacgcgaact ccgggacgct caacaacgtc
     9961 acgattaata agaactgtcg ggttctggga aaattgtccg cgaaccagat tgaaggcgat
    10021 ctcgttaaaa cagtgggcaa acctttccca cgggactccc gggcaccgga gaggtggcca
    10081 tcagggacca ttaccgtcag ggtttatgac gatcagccgt ttgatcggca aattgttatt
    10141 cccgcggtgg cgtttcgcgg tgctaaacat gagcggaaga ataacaatat ttattcgtca
    10201 tgccgcctga tagtgaagaa aaacggtgct gaaatttata accgaacgac cctggataat
    10261 acgctgatat atacgggtgt tattgatatg cctgccggtc acggtcacat gacgctggag
    10321 ttttctgtat cggcatggct ggtaaatggc tggtatccca cagcaagtat cagcgatttg
    10381 ctggttgttg tgatgaagaa atccacagca ggtatcagta tcagctgaat tttataaccc
    10441 agatacgggc accagaaatg gtgccttttt tattgcagaa aagcgagagg taattatgcg
    10501 taaactttat gctgccattt tgtccgcagc catctgtctg gctgtatccg gtgcgc
//
LOCUS       DADLTY010000144        10482 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.144, whole genome shotgun sequence.
ACCESSION   DADLTY010000144 DADLTY010000000
VERSION     DADLTY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10482
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.144"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..336)
                     /locus_tag="JN334_003169"
     CDS             complement(<1..336)
                     /locus_tag="JN334_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAY7439221.1"
                     /translation="MYTESNIKLSCNALWQKCVLATLYRAAATHPKIKLLQFKLIPCP
                     GMNKAIPSLIADFTGELHRLISEHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLM
                     VLLLNNDVWC"
     gene            complement(364..926)
                     /locus_tag="JN334_003170"
                     /pseudo
     CDS             complement(364..926)
                     /locus_tag="JN334_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1130..1336)
                     /locus_tag="JN334_003171"
     CDS             complement(1130..1336)
                     /locus_tag="JN334_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAY7439222.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1443..2006)
                     /locus_tag="JN334_003172"
     CDS             complement(1443..2006)
                     /locus_tag="JN334_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAY7439223.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3381..5216)
                     /locus_tag="JN334_003173"
     CDS             complement(3381..5216)
                     /locus_tag="JN334_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439224.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5317..5604
                     /locus_tag="JN334_003174"
     CDS             5317..5604
                     /locus_tag="JN334_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY7439225.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5576..7105
                     /locus_tag="JN334_003175"
     CDS             5576..7105
                     /locus_tag="JN334_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HAY7439226.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7275..8492)
                     /locus_tag="JN334_003176"
     CDS             complement(7275..8492)
                     /locus_tag="JN334_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7439227.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8859..9845)
                     /locus_tag="JN334_003177"
     CDS             complement(8859..9845)
                     /locus_tag="JN334_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439228.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9842..10166)
                     /locus_tag="JN334_003178"
                     /pseudo
     CDS             complement(9842..10166)
                     /locus_tag="JN334_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         3273 a         2398 c         1970 g         2841 t
ORIGIN      
        1 acaccagaca tcgttattaa gtagcagcac cataagataa ggagtggcat cgttagccac
       61 agcttcccta ctccagaggt aatataaagg ggtgggctcg actgatttat ctttacgtcg
      121 cttacactgc aaatgttcag aaattagtct atgcagctca ccagtaaaat cagcaatcag
      181 agaaggaatt gccttattca taccagggca aggtattaat ttaaactgta ataatttaat
      241 tttaggatgt gtagcagcag cccgatacag agttgcaagg acacactttt gccagagggc
      301 gttacaggaa agcttaatgt ttgattctgt atacataata aatcacctta tagttacaat
      361 aggtcaaaaa ccgctgtagc caaagttacg ctggcctgat gctttagtgt cgagcttcgt
      421 caaataatcc agacgtacca ataagcgctg gtactgctct gggaaattag gatcgttaat
      481 atccaggatg taacgtccat tagcagggaa atgaactaat ccctggtgat cttccagttc
      541 caaccctaag gcactgtacc aggctccggt aattaaccct ctcagggtgt cagagcgctc
      601 atagtttccc agatggtaat aggcgtcttt gttaaaaaaa ggcagacgtg ataatgacat
      661 ttaaaggact gcgtatattc tttcgcccag ataatacaaa ccgacgttgc gtaggtgcgt
      721 ttgccttgtt gttttttacg gtgttgatct gcattaattt tcgcattcag cgagttacgg
      781 aaacgagata tctctccggg gatcaggtta gggaaacagg taatgatatc cccgttgtca
      841 atcacaggag ggtaatggag atccccccgt attgctgtta ttctggaagt gtttttaata
      901 ttcgtattga tagttctcag agatatatca cactctagat attttaatta agtatcagtt
      961 cgatatcggc atattgtaca ctacctcagg tagatgatga gatcagatac agggatcaac
     1021 tgacggtgag cgcagtcccc acattttatc cagggcttac tgcatattcc caacagccat
     1081 tcgttagcca ggtagacgaa taccctgatt tgccgctggt tttgctttcc tatcgaaggg
     1141 cccatacatc atcacgccca cgaaacaacc gacgaaataa cgcaactttc tcatccgtgg
     1201 ataatacgaa aacgcactgc acagggctct gcggtttacg tcgccattca atcccatgag
     1261 cttcaagtaa tgagaccagc cgggtatttt ctgcccgcag tactgctagt tcatcgctgt
     1321 cagatttata aaccatatgc gttatcactt gtacctgtct cccggcggca ccacgactga
     1381 aattcgtgtg aaggcaaatc cacgcgataa cttacgatag ttcggacata agcccccata
     1441 attcagttat gtcgtggtcg accccggtta cttttttcgg gaagcctgct acggcccaga
     1501 ataataccgt gttctttctg atcctgttta gccagatagc taagggcgta acgtaagtcg
     1561 tctaccgcag atttatcact atagtgaatc acgtgatcga tacgcaccgg gtatttgtct
     1621 ttagcccggc acagatgaaa ataaccctcc ccttccgtga tccgcctcca gatatctccc
     1681 agttgcctgg atatctgata agagttcttg tgacgttgtc catccaggta gccgataaaa
     1741 tgaatatgaa gccctttgtc tggtgtatat tccattaccc agtaatatcc cgccaacatc
     1801 gtctgggttt cgctaagtaa acggtatatt tccatacaca tactgtgctt acaggaatgc
     1861 ccgaaactgg gcgtgtcttt cctgtaagca aaatcaattc tgaaaggtaa cagtttagaa
     1921 aaacgttgaa acataccatc catgtgttca ttcacgtctt tcagaatcat gaaatccatt
     1981 ttgtaattgg gattggcatt atacatttta tagtccttac tttataaaac tcacaggaag
     2041 agagaattac gtaaatactg agagtagaga aacgccgtac cacggcatta ataaacgaag
     2101 taaaccagca gcacagacaa cctgataaaa ctgttttgta ttaccgcatt actgtgatga
     2161 ggtaatactt cagaatacag tgagactctc ttaaactgag aaccagtacg aattacaatg
     2221 gaaaaacaca cctcatagaa ttgagtttca ggaagcacca ctaaattctt tacaggaagt
     2281 aatctgacat tcacaaaact cagtatcggc tagctacagg caacatcgct attaatattg
     2341 tcgaaccatt aagtaaagcc gagagagacc acgggtattt aatcattagc ataaaagaat
     2401 aatggtatac agaacatacc cacacaccat aagaagattg ttccatacag aaaagatcat
     2461 atttacctat acctataaac tgtaacggag tcataacacg ctctaatagt gttccgatac
     2521 gtaaccagtg ttaagtacgt acaggacaac attacacaca taattcacca tacagcttcc
     2581 cccatatgct tcactgcgta gtgtatggag atatatgcat tacacacata cagaatactt
     2641 agcataaaga ggagtgacat taccacgtaa agagataagt tatacaacaa acatacaaac
     2701 aatacggcac agacacacaa taccataact ccaactaata atactaatta accttaatta
     2761 tttatggtat atgaaataaa tgtgattctc ccggaactaa ctaacctaac cagacagatg
     2821 atcactgtat aaaaacttca gttaatactc atttgttttg tcgactcagt atataatgag
     2881 tgaccagtaa tcaggataat cacacttaca taacctgtaa aaaagaacaa gaagaataag
     2941 ccactaacat aatgcaaaat ggcatctgaa tcgatcgcag tttggcattc gaatcgatca
     3001 tgatttggcc caatccgtga tcgaatagag cggaatacgc aacataattc accagacaag
     3061 agcttacacc gaattacaga ttatgtatta atgtcaaaac tggcatattt tacagataca
     3121 atgaacatgc aacccatcaa tctaataggg gcggtatgta catctgccca tgaatgggat
     3181 ggcgctaaga atacaaaaca attagacata gatcacaaaa acacaccatt ctaattgaaa
     3241 aacaaaacaa acaaataaat catactatta tagaattttc tcttgccaga catagataat
     3301 acccctgcta attttgctaa tggttagcaa aattagcagg gtatataaag attattgacc
     3361 caaaacacac tcagatgaat tcacgacagc tcaggagggt tattcctgat gtcatcttca
     3421 caaataatgg ttaacgcatc atatatcatt ctgcgtctgt tcctgttttc ggccttatcg
     3481 ccatacaaca tcatcacatt tttaatttca tcgaccggtg atttcacata gtgaactaat
     3541 acaccaagct tctcaacaca ttgcagtaaa gtatatttct tactgcctgt gaaaagccct
     3601 atcatagttt ctttacgttc gcgactggat aatactttgt tttccataat gacttctgcc
     3661 agcgtcttac catcataaac atactcttta tctatcagaa tatttatctt atccagcacc
     3721 ttctctacgt catcaagcaa acctgtaata tatgggggcg caatatatgg tttcgtactg
     3781 atcttttcaa gcatacaatt atacatctta ataacacgca tcagggtaaa attgttcgca
     3841 tcatactgta tttcaccctc cttatataca ggatcaataa gtggtatttt ttcaataaat
     3901 gcatcctgat aggcttttac atggtcaagc caataaaaca acgagccata ctttatattt
     3961 tttctattta ctttaacctc cagtgctgtc cgaagcaacg ataatgcccc cctgacatat
     4021 cctgaaggta attcaccaaa caatcctttt ctttccatgt catcataatg ctgcagcaca
     4081 tcattaattt tatttttcct tatcagacaa atgtacaccg caagtgtttc gtagcagctg
     4141 gtcacatgaa cagcctcatt ctgcaactgc ccaaaatagc ttataatctt tttcagatat
     4201 gcgtcatcac aaccattctt atcattaata ttaaatacaa gatctttcag acgggaaata
     4261 tattcctgag atttatcatc aagtgaaaca ctataacgag acaatgttcc ttctgcactg
     4321 ttttgaaact tataaaacat ttccagtaat ttttttgcat aaggcgttgt atcaaagctg
     4381 gtaaagtcat aaagatattg cgcagcgtaa ccatacaaac catgaatcca gagtccttct
     4441 ctcaccaaca ctctgagata atatgcaagg ctttgaagta tcttcgtctt tcgttgtata
     4501 tcaagtaaga cacttaatga ataaaatgca ttatagaatt tatcaatatt cctgagcact
     4561 ttctccagtt catccttatc cagcttcttc tcccggtaat cctttttata ttcatttttg
     4621 atagccatga tatattgttg catcaggctc ttgcttttcc cgttatcctt ccgtatagtt
     4681 cctgtatatt tccccagagc atacagcttt ccattttcat gaaaatcttc cttgttgaat
     4741 cccagttcag ccagatattc ccgaagataa cgtttaacag cagcttcatc gctttctgta
     4801 tcagcgtgat caagacaagg acaatacgat ttctgtaaaa aattataaat atgataataa
     4861 aaagcagagt cattctctgg tctgtatgta ttatacatat ctacaacaag aaaaataacc
     4921 ccaaaagtgc gcagacaata gctgcgaagg aattcaggtt ttagtgtgga atgcactgga
     4981 taagaattca tcatgtaata aaaatgttca tatcgggcat atatgatccg ccgttgctcc
     5041 tgactgaaca ccccttcagc atcaataata cgactgatct cattataacg ttgatcccag
     5101 aatttttgat gcatttcaaa attggtactc ttccgcttgt gtgccggatt ctcgccttcc
     5161 ttaaacaacc ccaaaacagt acaaatatca tacaacatat ttgtaaatgt gcgcatatgc
     5221 tgtgctctcc ggttggtttg cattttatcg cagaggacat tctgagctta acgtatcaaa
     5281 ctgacacaac gacatgcaat gcatatggag aacaatatgt caaatcaaac cggacagctt
     5341 tacacgcctt cacgcatcct gcgtatggct gaacttaccc gactgctggg tatatcaaga
     5401 tctactattt acgtgaaaat aaatccggcc tcgaaatacc acgaccctac ctttcccaaa
     5461 ccaataaggc ttggtagtgc atccgtaggc tggcgggcat ctgccatcga tgagtggctg
     5521 atgttacata cggcaccagc ctggtctgaa cctgaaaaca aactaaagga acttaatgat
     5581 gacgacaaaa ctatcactcc ctgaacttga cacaactaaa ctgcctgaca ggtgccaggc
     5641 attacttgat gaaatgcatg aagaaacagg aatacgccgt gaaatacttc tgtctgttat
     5701 gctgacggtc atggctgcct cggtccagga tacgcatgaa gttgaacttt ccggagggca
     5761 acgtaccagc cttcagatat ttatgtgtct ttcatcagca tcaggcagtg gaaaaacctc
     5821 tgcctgcgca aaattaatcg ctcctgttca tgaaacagaa gaagaactac atcaggcata
     5881 tatcgacgat aaaaaaaact atgaccgaat gatggaaatg tggacaacag ataaaaaaat
     5941 cctggagcgg agatataaaa aggaaattga aagatccccg gaaaatgctg ctgctgcacg
     6001 agcagcactt gaaaagtgta tagcaaataa acccgaacct cccgtacaac aggttctcat
     6061 cgtgaatgac gcgacaccag aagggatagc tttgaaactc agccagtcac cctctcttct
     6121 gttgctgtct gatgaaggag gtactattct ggataagcgt ttcgaacgca aatcggcact
     6181 gtacaacaca ttatggagtg ggcaaccagt caacgttgaa cgggcatcca gacccgggtt
     6241 ccggctgaat gatgtccgtc tcactatact tatactgact caaccggtaa tatttaataa
     6301 gttttttact ctcactggcg atcagatccg gggcaatggc tttctggccc ggttgctatt
     6361 ttgcgaaccc ggagcaatta aaataatgac gacagaaccg catactgcca ctcctgttgt
     6421 gacgcaacaa tgtgcaagct gttttcgtaa gaaaagtttt ggcagtcaga tcagagactc
     6481 tctgagagca tccagagaaa gacgtgcaaa aagtaaagag cgcatctgca tgacattatc
     6541 aaatgcagca tcccgcgcac tggagcgctt tcacaaagaa aatatggata ctgtcagaca
     6601 gaatcctcat atgggtacat ttgaagacat tattgtcaga aaaagagagc aggttgtcag
     6661 gatagcagca cttctggaac tggagaaaga tccagactcg acagtaataa cgctggaaag
     6721 tactaatagc gccatttatc tcattgaatt ttatttcaag catcttatat ataaacttga
     6781 atcccttcgg gaaatatccc cggctgagaa acttgataaa tggcttcaga aaagaattat
     6841 cacaacagca ggttacatat tccagaaaag ttatatttta caatatgccc catatgctct
     6901 caggaaaaaa tgtgttctgg atgaagcact tgacatactg gcggaacaaa ggaaaataag
     6961 gattgatgat aacctggttg tttatatagg aaacacgatt acaccatccg aactggcaaa
     7021 aaaactaaat attcctgcat tcgatgccgg agttttcatt tgtgatcatc aaaacatact
     7081 gaagtatcac cgaaacagac tatagatact atcggacaaa aaataaatta tcacaagtgt
     7141 attattcgtt ataataatga ataatcgtta tctgagtgca atgccaggtt ttcattacga
     7201 actggcattg catttatttt tcaaaaaaga aagcagtaac catttggcta ctctgagtat
     7261 gccagtccat gaaactaagc gaattcctta gtgtttttag caagttgaga catcacataa
     7321 tccgcccatg cctgcatcat cggtacacgc tgctccagta gatcagtacg atgatatgcc
     7381 gcctcaacct tatttttcag cgtatgagcg agcgcccttt ccgccagatc ccgcgaatac
     7441 ccctgttcac tacaccagtc cctgaatgtt gagcgaaaac catgtgccgt ggcaactcgc
     7501 cccggaatgt cactgacggc tttcttttta cgcagaaaac ttgtcaacac catatcggaa
     7561 aggatctgct gctttctggg tgaagggaac accagttcat catgcaggcc acgtatattt
     7621 tccagaatgt aaatagcctg ccgggataaa ggaacacgat gctgtagcct agctttcatt
     7681 ctttctgcag gtatagtcca tacccgctta tgaaaatcaa tttcagccca gcgcattccc
     7741 ctggcttcgc ccgagcgagt tgctgtaagt atcaccatta ataacagtgc gcgggtaaca
     7801 ttataaggtt catcactata cacactggtc gccacaaaaa gcggtaactg cctccagggc
     7861 attgcgggtt ggtgttcatc acgtcctctt gtctgctgag gaagcaaatg gtcaaccaca
     7921 tcaacaggat ttgccacaca aaaaccgtgc gcccatcccc actgcataac aacatgaatg
     7981 cgctgtttaa cccggcttgc cgtttctgac aaggttaacc agactggacg cagtgtttct
     8041 gccacgtccg cagccgtaat cgaatccagc gtttttgctc ccagttgagg aaacgcgtaa
     8101 ttctcaagcg tcgataacca ctgccttaca tgctttggat tctcccatcc aggagacagt
     8161 tctgcatgta cacgcctggc tgcatcggca aatgttggga taacgacttt ctcagattca
     8221 gcctttttaa tctccagagg atcatcacct gcagcaagtt gctctcgcat tatccgggca
     8281 gtacgtgcag cttcagcaat actgacctct gggtaagttc ccaatccagc attacgtctt
     8341 ttttgtgtca ccggacttac ataacgaaaa acccatttcc cccgcccctt tactgaagaa
     8401 ggatgaaggg tcagtccggt aattccccca tggggcaatg gtttgtcatc aggtttgata
     8461 tgtcttgctt tcgtatccgt caatactgcc atacgctaat tcccgtcact ctggtatgcc
     8521 atccagtatg ccattagtcc ctcgcttcac taagaataca ttagataaca tcggacaaca
     8581 aaaagtgtaa tacattgttt ataaagaaat tatataacac ccctggatta agtcagattt
     8641 atttcaggcg gtccccctca ccgccatatt taaagaagag cgcgtacgaa agtacgggct
     8701 tttttttcgt atattgtaca caccgggtgc ccccgcccac gtctgttagg gcgaggggaa
     8761 attgtgcaca tcaactgtct ggttactcag tcaacaatca aaatgcgtaa gatagtacag
     8821 aacatatatg cgttatctgg tcacttcctg taacttcctc acttattaaa atgcccccaa
     8881 cggaaataca tgtcacatgc catcataaaa tcgttatgcc agaggaatcg acaatggaaa
     8941 aaatagtaaa ttaagcacgg tacatagcag agtaaaaaat aggcttcaaa ccaaatccgc
     9001 cattccacaa gcgttgccaa taccgacacc agcgtaatcc acatcatttc aataacccgc
     9061 aatatgaata accgaagcca aagcggcttg gcaaaaaagg tcgacaacgt attttcactg
     9121 gagaaaaaca gatcgatttc ctgcaaaaaa aacctgccca gaaaagcggg ccgcttggag
     9181 aagcgcagaa aaaacagatt aatcgccaac actacgccac aaagaatgag caatgcaatt
     9241 atcgatttaa acgttaacca ctgcccctct gtcacaaaag aatcactgtt acgcagcggg
     9301 ctgatagtaa gcaggttaac aaccgttgag atgcacaccg aaagcagcac atagcgagtc
     9361 agacttttat attttacttt tttcgctact gaaatagcga cctgcttgac ataactggtg
     9421 cggatcatcc gaaacagcag aaagatgacc aacggcccaa gtaacgaaat caggaacagc
     9481 gcatataacg agggtagaag ctggtatagc ccccaactca ttgcactcaa catgattaac
     9541 gacagaaatc ctgccaccag aggttgtgta agaaataatt ctattttgtg gttctgcttt
     9601 ttcttaaacc acgaaaaatc gatattcttc gtgacgcatt tataagccca gtacgccttg
     9661 agatcaaaaa taaaattata cagcaccagg gccagaatcc cactaactac accaatatcc
     9721 tctgatggaa aagtaaaccc tgcatttttc caataaaagc ctatcagaat aagtccaata
     9781 gcataaatgt atatcagatt tcgtttatcc tgaaagcgaa taaaagttaa ataatcagga
     9841 atcatttgaa tacctttgca tcactaaatg ctttacgaca atgcataact cgttgctgat
     9901 ccacgccagc aaaatcattt aacatattcc ccaaataggg cttattactt atttcaagga
     9961 aagctaccgg ggttggcata ttcagaatat gtatcgagag attcagtcca tttcaccgta
    10021 tgagttaagt gtaatgagag attttctttg ataattgacg gagcgacctc gctttttgct
    10081 gttacgttca tcagaacctg atgctctggc gaagcgatat ccagtccggc aagataatcg
    10141 cgcattgcct gaacgccgtc ttccatcaaa cgcgtgtgcc aggcaccgct cacaccgagt
    10201 ttaaccggtt cgtatccagc ggccatcagc agcgtggtaa attcattcaa cgcagcctgc
    10261 gttcccccaa taacctgctg gcgcggcgtg ttatcacagc taatatccag cgcaatgcct
    10321 gattccgtaa tcatcgtctg cagttgttcg cggttgatgc ctttaaccgc ctgatcttac
    10381 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg acttacatga
    10441 caaaaacagt atcaaccagt aaaaaacccc gtaaacagca tt
//
LOCUS       DADLTY010000145        10464 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.145, whole genome shotgun sequence.
ACCESSION   DADLTY010000145 DADLTY010000000
VERSION     DADLTY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.145"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            3..203
                     /gene="cspG"
                     /locus_tag="JN334_003179"
     CDS             3..203
                     /gene="cspG"
                     /locus_tag="JN334_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HAY7439229.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            214..402
                     /locus_tag="JN334_003180"
     CDS             214..402
                     /locus_tag="JN334_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAY7439230.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            377..607
                     /gene="ymcE"
                     /locus_tag="JN334_003181"
     CDS             377..607
                     /gene="ymcE"
                     /locus_tag="JN334_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HAY7439231.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            597..770
                     /locus_tag="JN334_003182"
     CDS             597..770
                     /locus_tag="JN334_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HAY7439232.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(819..1892)
                     /locus_tag="JN334_003183"
     CDS             complement(819..1892)
                     /locus_tag="JN334_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HAY7439233.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1994..4660)
                     /gene="torS"
                     /locus_tag="JN334_003184"
                     /pseudo
     CDS             complement(1994..4660)
                     /gene="torS"
                     /locus_tag="JN334_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309175.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4788..5816
                     /gene="torT"
                     /locus_tag="JN334_003185"
     CDS             4788..5816
                     /gene="torT"
                     /locus_tag="JN334_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HAY7439234.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5789..6481)
                     /gene="torR"
                     /locus_tag="JN334_003186"
     CDS             complement(5789..6481)
                     /gene="torR"
                     /locus_tag="JN334_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HAY7439235.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6568..7740
                     /gene="torC"
                     /locus_tag="JN334_003187"
     CDS             6568..7740
                     /gene="torC"
                     /locus_tag="JN334_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HAY7439236.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7740..10280
                     /gene="torA"
                     /locus_tag="JN334_003188"
                     /pseudo
     CDS             7740..10280
                     /gene="torA"
                     /locus_tag="JN334_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10277..>10464
                     /gene="torD"
                     /locus_tag="JN334_003189"
     CDS             10277..>10464
                     /gene="torD"
                     /locus_tag="JN334_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HAY7439237.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2524 a         2685 c         2788 g         2467 t
ORIGIN      
        1 aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg
       61 atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat gaattccgca
      121 cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg
      181 cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt
      241 ccatgttgtc atggttcaca gtaccgtaca tcggcattcg atgtgacgga gcgaaaccct
      301 ttgggcgcta agtgtatttt ttgtaaatcg actatgatca cctttgataa cgtcgcgctg
      361 caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcgtgg
      421 agcctttttt atatctgcct tatttttctt caacgctgta tgtatagtaa gcgataacct
      481 gttgattatt gaatctttcg ggaagatggc ttataacatt tcttacctga ccagggtacc
      541 gggaaccaac accttactgg cgtgttgctg tctttcaaga ccagacgagg ttaacagtga
      601 atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc gcgcaaaaaa
      661 tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc atcgcacgag
      721 aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga ttttgtgaac
      781 atcaccccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat cattggcgct
      841 tgtggaatta agcgtcggta caattcctcc ggcaccgggc tttgccatac tcccgcatac
      901 attgcgtaac caatcaccgc aaacataatc cccagaacca gtagcgtcat taaccagcca
      961 gacaacgcaa aggctttttt atttgctgca ggtttttgca gtgaaaaggt caatgttgag
     1021 gctaccggac atgactctac gcaagtcata cagccggtac attccactgt tcgtacctga
     1081 attaatttat ctaccgggat ccgtgatggg cacttttttg cgcatttgcc acaatcgata
     1141 caactttcgg cattgcgacg aatcttaaac ggcgacaata gcgaaaccac gcccatcagc
     1201 gcgccatatg ggcaaagata acgacaccag gcatggcgaa taaacaggct ggcaatcagc
     1261 aaaacggtca cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc gagcatttta
     1321 acgtccatca ccacgctata gggcgacaac ataaaatagt gaatcgcctg agcgggcatc
     1381 aataacgcga tatagagaaa taaactcaac agcaaatact tcacgccgcg cagaggaata
     1441 tccagccagc ggggaaggac acattgccga ccaaacagtt tgttaccgag atcgccgatt
     1501 aattcagaaa gcgtaccaac cgggcataac catgagcaaa aggccttttt gagtaataga
     1561 ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga cggtaactga
     1621 cctgttgcaa ggctatattt cagattcatc agaccggcaa tcggtagcca gccttcgata
     1681 cctcccggtc tggcgacaaa tgtcgtacta cctgccgttt cgtaatagcg cacccaatac
     1741 cagaacgtga tggcaatata aatattcatt gccaatagta agaattgcgt cgctttacgc
     1801 caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc
     1861 cgctgccagc gggttctttt tttctctgcc atgattttgc cagtccgtta agttgcatac
     1921 caaatgccac tattctagtt attcttaact ggctgatatt gattcaaatc gcgttcaggt
     1981 ctttcttatt caatcatgct tccagagcgg caacactgcg tgtaatttct tcgtgtggaa
     2041 ggggggcaga taatggctgc tgctccagtt gtgcgcatag ctgactggcg ctacgcattc
     2101 ccagactcga gcaactgctt tttagctgat gcgcggcacg ctttattttc tcgctgtcct
     2161 ggctggcgcg ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt ttaaataatg
     2221 ccagccattc gtggatcttc tccgtcccca ttaactgagc gtcttcattg agttgcgaga
     2281 catccagtgg tagatcgtta ttggcttgca gttggagata gtgcgccagt aactgaccga
     2341 gcacttcacg cggcaccggt ttagggataa tcccgcgaaa tagtgaactg gtacgctggc
     2401 gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac ggatattgct
     2461 gtgccagttg ccgggcaagg gtaatgccgt cgatatccgg cagatcaaaa tccaccagtg
     2521 cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca ttgccaatag
     2581 caacaacctg cgcaccactg gtgttcagca tctcaacggt aattcgctgg gttagcgggt
     2641 tatcttcaat taacagcaaa cgtaaaccgt caagacgcac cgcctgattg gctgtttttg
     2701 gcacgggtgc agtggcaaca cgtaacggca agcgtaaaca aaaacagctt ccaacctccg
     2761 gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc cagacggcta ctgatagtca
     2821 gccccagccc ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg aagatttctg
     2881 ccagtttcgc gggatcaata ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc
     2941 catcagtgcg actacgcagg ataatgtgcc cttcgtcagt aaaacgcagg gcattgctca
     3001 ataggttggt aatgacctga cgaatacgtc gtggatcgcc cattaacgcg ctcggtacat
     3061 catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt
     3121 gcagggtact ttccagcagc gggcgcggtt caaagggctc atcgctgact gaaacatttt
     3181 tgccacctgc ttcgatagcc gaataatcga gaatatcgtt gaggatggtc agcaacgatt
     3241 cgccagagtc ggtaatcgcc tgcaaatcat cacgctgggc gttaagtgcg gggttatctg
     3301 ccagcagttg agcagtgccg agaataccgt acagcggtgt gcggatctca tggctcatcg
     3361 ccgtcagaaa cgccgatttt gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt
     3421 gttctatcac cagttcctgc aattcagctg tacgcgcttt gacctgcgcc gccagctgtt
     3481 cgcggtggcg attcagtgca tgaacactgc tgcgaaacgc atccatcagc cgcccgatgg
     3541 tatccagctc ccgtacgcca gcggtttccg ggaaagggga gtcaatatca ccgtccagca
     3601 gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt gaatgagcga taaaccacgc
     3661 gccagaggat cataatcagt gcgcaaagtg aaaccatccc cagcaataac aggctgtatt
     3721 gcccgcgtgc actggctttt tccagatgtg ccagtccatg ctgattgtgc agctcaatgg
     3781 tgtcgaccag ctgactgact tcgctactaa actgcgcgaa ctgggcgata ttattttggg
     3841 cgagagtttg caggtgattg ctgatttcac tgtcctgctg gaacagcgcc aacagatcgc
     3901 tatattggct aacggtagtt aacgttgttg cgacctgcgc acgaactccc ggatcttcaa
     3961 tgcgtatttg ccgacgttgc agaattttca ccgcattatt gagctgcttt tccagcgttg
     4021 gtgcattttt ctggatctgc tccagcccca gattcatcac catttgctgc acccgcagag
     4081 cgctaaggcg cagttcattc atctggttaa catactcaag atcgatatca atcagccgat
     4141 cgagtgcact ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa atcccggcct
     4201 gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc ttgctccagg cgtgcgatct
     4261 catcggcggc agctatctgc tgactgagtt gccgttgttg ctggcgtagt tgcagacgcc
     4321 gccccaccag ttccccttgc tgacgtaatg aacgggagat ctcctgctcc tgttgttcaa
     4381 tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta atcttcaggc
     4441 tttatgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg gcactggtca
     4501 ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc gccatattca
     4561 tggtaggaat caatgcctga gtattgtctt tttccacctg gctgataaag cgcaggttgt
     4621 accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag cccatccaga
     4681 gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaag gttgagtcgc
     4741 aaagtggaat gcatctagca taaagcctta ttattgatga ggctatcatg cgcgtactgc
     4801 tatttttact tctttccctt ttcatgttgt cggcattttc ggctgataac ctgttgcgct
     4861 ggcatgatgc gcagcatttc acggtgcaag cctctatgcc gcttaaagcc aaacgcgtat
     4921 ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg ttgaactatg
     4981 gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag gcaggcggct
     5041 acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg
     5101 ccattttgct cggtagtagc acgacgtcat ttcccgacct gcaaaagcag gtagcaaatc
     5161 tgccggtgat cgaactggta aatgctcttg atgatcccca ggtgaaaagc cgcgttggtg
     5221 taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg gcgcacggta
     5281 aaccactgaa cgtgctgctg atgcccggac ccgataacgc cgggggcagt aaggagatgg
     5341 tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat attgcgcttg
     5401 gtgataacga tattgaaatc cagcgcaacc tgttgcagga gatgctggaa cgccacccag
     5461 aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt gaagggcgta
     5521 acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc
     5581 tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag ggggaactgg
     5641 cagttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat gtcagcccac
     5701 cgattttagt tctgacgccg aaaaatgccg accgagaaca tatccgccgc tcgctgtcac
     5761 cggggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag aaataacctt
     5821 caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga cgaattaaca
     5881 catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata cgcagcagac
     5941 gctcacggct gagaatttcg cccggattag tcacaaatgc caccaacatt tcatactctg
     6001 cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac acattcaggc
     6061 aataacccgc aaagcgatag cagtagtcct gagtgtgcgg ttgagcttgt cgcgcgaggt
     6121 cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc agcggtttgg
     6181 tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga tcgctgcgtc
     6241 cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt aacatcaggc
     6301 cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga ttctgcataa
     6361 tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt atacccctcc tgagtgaagt
     6421 aggattgtaa tcgcgcctgg gtaaccggct tatcttcaac aataacaatg tgatgtggca
     6481 tcagagggtt ttactcattc tgttcatatc tgttcatatt tgctcattaa gatcgcttca
     6541 ctaaaccata attctacagg ggttattatg cggaaactct ggaacgcgct acgccgaccc
     6601 agtgctcgtt ggtcggtact ggcgctggtc gcaattggga ttgtgattgg cattgcgctg
     6661 attgtattgc cacacgttgg gatcaaagtc accagcacaa ccgaattttg tgtcagttgc
     6721 cacagtatgc aaccggtgta tgaagaatat aaacagtcgg tgcatttcca gaacgcctcc
     6781 ggcgtgcgag ctgaatgcca tgactgtcat atcccgccgg atattccagg catggtgaag
     6841 cgcaaactgg aagcgagcaa cgacatttac cagaccttta ttgcccactc cattgataca
     6901 cctgaaaaat tcgaagccaa acgcgcggaa cttgccgagc gtgaatgggc gcgaatgaaa
     6961 gaaaacaact cggcaacctg tcgctcctgc cataactacg atgcgatgga tcatgccagg
     7021 cagcatcctg aagcggcacg tcagatgaag gtggcagcga aagataatca atcctgcatc
     7081 gactgtcata aaggtattgc ccaccagtta ccggatatga gtagcggctt ccgtaagcag
     7141 ttcgatgagc tgcgcgccag tgctaatgac agtggtgaca cgctgtactc tattgatatt
     7201 aagccaattt atgcggcgaa aggcgataaa gaagcctctg gttctctgct gcctgcttcg
     7261 gaagtgaaag tccttaaacg tgacggcgac tggctgcaaa ttgaaattac cggctggacg
     7321 gaaagcgccg gacgtcagcg tgtactcacc caattcccag gtaaacgcat ctttgttgcc
     7381 tcgattcgtg gtgatgtgca gcagcaggtg aaaacgctgg agaaaaccac cgttgccgac
     7441 accaataccg agtggagcaa gttgcaggcc accgcgtgga tgaagaaagg cgacatggtg
     7501 aacgatatca aaccgatctg ggcctatgcg gattcgttgt acaacggcac ctgtaacgag
     7561 tgccacggcg caccggaaat cgcccacttt gacgctaacg gttggatcgg cacgctcaac
     7621 ggcatgattg gctttaccag tctcgataaa cgtgaagaac gcactttgtt gaaatatctg
     7681 caaatgaatg cgtctgacac cgcaggtaag gctcacggcg ataagaagga agaaaaataa
     7741 tgaacaataa cgatctcttt caggcatcac gtcggcgttt tctggcacaa ctcggcggct
     7801 taaccgtcgc cgggatgctg gggccgtcat tgttaacgcc gcgccgtgca actgcggcgc
     7861 aagcggcgac tgaggctgtc atctcgaaag agggcattct taccgggtcg cactgggggg
     7921 ctatccgcgc gatggtgaag gatggtcgct ttgtggcggc aaaaccgttc gaactggata
     7981 aatatccgtc gaaaatgatc gccggattgc cggatcacgt acacaacgcg gcacgtattc
     8041 gttatccgat ggtacgcgtg gactggctgc gtaagcgcca tctgagcgac acctcccagc
     8101 gcggtgataa ccgttttgtg cgcgtgagct gggatgaagc cctcgacatg ttctatgaag
     8161 aactggaacg cgtacagaaa actcacgggc cgagtgcctt gctgaccgcc agtggttggc
     8221 aatcgacggg gatgttccat aacgcttcgg ggatgctggc gaaagctatt gccttgcatg
     8281 gtaatagcgt tggtacgggc ggagattact ctaccggtgc tgcgcaggtg atcctgccgc
     8341 gcgtagtcgg ttcaatggaa gtgtatgaac agcaaacctc ctggccgctg gtattgcaga
     8401 acagcaaaac cattgtgctg tggggctccg atttgctgaa aaaccagcaa gcgaactggt
     8461 ggtgcccgga tcacgatgtt tatgaatatt acgagcagct aaaagcgaaa gtcgccgccg
     8521 gtgaagttga ggtcatcagc atcgatccgg ttgtcacatc cacccatgag tatctggggc
     8581 gcgagcatgt gaagcacatt gcggttaacc cgcaaactga cgtgccgctg caactggcgc
     8641 tggcacatac gctgtacagt gaaaacctgt acgacaaaaa cttcctcgct aactactgtg
     8701 tgggttttga gcagttcctg ccgtatctgc tgggtgagaa agacggtcag ccgaaagatg
     8761 ccgcatgggc tgaaaaactg accggcattg atgccgaaac cattcgtggg ctggcgcggc
     8821 agatggcggc gaacagaacg caaattattg ctggctggtg cgtgcagcgt atgcagcacg
     8881 gtgaacagtg ggcgtggatg attgtggttc tggcggcgat gctggggcaa attggcctgc
     8941 caggtggtgg ttttggctgg cactacaacg gcgcaggcac gccggggcgt aaaggcgtta
     9001 ttctgagtgg tttctccggc tctacgtcga ttccgcctgt tcacgacaac agtgactaca
     9061 aaggctacag cagcactatt ccgattgccc gttttatcga tgcgatcctc gaaccgggga
     9121 aagtgatcaa ctggaacggt aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg
     9181 ccggaactaa cccattccat cgccatcagc agatcaaccg cattattgaa ggctggcgca
     9241 agctggaaac ggttatcgcc atagataacc agtggacctc aacctgccgc tttgccgata
     9301 tcgtgctgcc tgcgaccacg cagtttgagc gtaacgatct cgaccagtac ggcaaccact
     9361 ccaaccgtgg cattatcgcc atgaaacagg tggtgccgcc gtagttcgag gcgcgcaacg
     9421 actttgatat tttccgcgag ctgtgccgtc gctttaatcg tgaagaagcc tttaccgaag
     9481 ggctggacga aatgggctgg ctgaaacgca tctggcagga aggtgtacaa caaggcaaag
     9541 gacgcggcgt tcatctgcca gcgtttgatg acttctggaa taacaaagag tacgtcgagt
     9601 ttgaccatcc gcagatgttt gttcgccacc aggcattccg cgaagatccg gatctcgaac
     9661 cgctgggcac gccgagtggc ctgattgaga tctactcgaa aaccatcgcc gatatgaact
     9721 acgacgattg tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc
     9781 ccggctcgca gaagtatccg ttgcatctgc aatctgtgca tccggatttc cgacttcact
     9841 cgcagctatg tgagtcggaa acgctgcgtc agcaatatac ggtagcgggt aaagagccag
     9901 tgttcattaa cccgcaggat gccagcgcgc gcggtattcg taacggtgat gtggtacgcg
     9961 tctttaacgc tcgcggtcag gtgttggcag gagcagtagt ttctgaccgc tatgcacccg
    10021 gcgtggcgcg aattcacgaa ggggcatggt acgatccaga taaaggcggc gagcctggtg
    10081 cgctgtgcaa atacggtaac cccaacgtgc tgaccatcga catcgggact tcgcagctgg
    10141 cgcaggcgac cagtgcgcac actacgctgg tggaaattga gaagtacaat ggaacagtgg
    10201 agcaggtgac ggcgtttaac ggccccgtgg agatggtggc gcagtgcgaa tatgttcccg
    10261 cgtcgcaggt gaaatcatga ccacgctgac agcacaacag attgcctgtg tttacgcctg
    10321 gctggcgcag ttgttctccc gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc
    10381 gcagatggct gaatggtttt cgttgctgaa aagcgaaccg ccgctcactg cggcggtgaa
    10441 tgatctggaa aacagtattg ccac
//
LOCUS       DADLTY010000146        10372 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.146, whole genome shotgun sequence.
ACCESSION   DADLTY010000146 DADLTY010000000
VERSION     DADLTY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.146"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="JN334_003190"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="JN334_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAY7439238.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="JN334_003191"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="JN334_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HAY7439239.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="JN334_003192"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="JN334_003192"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAY7439240.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="JN334_003193"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="JN334_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAY7439241.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="JN334_003194"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="JN334_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAY7439242.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="JN334_003195"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="JN334_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAY7439243.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="JN334_003196"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="JN334_003196"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAY7439244.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="JN334_003197"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="JN334_003197"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAY7439245.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="JN334_003198"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="JN334_003198"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAY7439246.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="JN334_003199"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="JN334_003199"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAY7439247.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..>10372
                     /locus_tag="JN334_003200"
     CDS             10299..>10372
                     /locus_tag="JN334_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit B"
                     /protein_id="HAY7439248.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYV"
BASE COUNT         2574 a         2565 c         2629 g         2604 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gt
//
LOCUS       DADLTY010000147        10360 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.147, whole genome shotgun sequence.
ACCESSION   DADLTY010000147 DADLTY010000000
VERSION     DADLTY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.147"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(91..951)
                     /locus_tag="JN334_003201"
     CDS             complement(91..951)
                     /locus_tag="JN334_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAY7439249.1"
                     /translation="MSNLATVTHLRPSQRPVERRVAEVEDGYTRLANALYEELIGADL
                     TKNQSKVAHAICRKTYGYGKKMDRISDSQLAQITRLPRQKVNKAKNELIAMKVILREG
                     QQIGPNKNIEEWQIEGCHYSGDNVTALVTKSVTKTVTALSPKQGHTKETITKEKRNNK
                     NTMSESVRTECEKSHDRHEETDKAFEEIFWCAGMRKAGKKNAASAFRTQFREWRKTTR
                     GTASEFATMLAEDIACRNGKQFGFDRLLPSSYLNGQRWNDEKPETIQPQSKPSSAITV
                     SKTGYVFFDR"
     gene            complement(944..1090)
                     /locus_tag="JN334_003202"
     CDS             complement(944..1090)
                     /locus_tag="JN334_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 domain-containing protein"
                     /protein_id="HAY7439250.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            complement(1125..1406)
                     /locus_tag="JN334_003203"
     CDS             complement(1125..1406)
                     /locus_tag="JN334_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015995142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439251.1"
                     /translation="MGTIATKSKKAARIESALLNKLALMGQKTFARAMGVPEYQVSRW
                     KNGFFSQVSMMLAVLEYGIEDDEMAELTKRLADYLTKEKAPKNGEFFEA"
     gene            complement(1523..1738)
                     /locus_tag="JN334_003204"
     CDS             complement(1523..1738)
                     /locus_tag="JN334_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7439252.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            1842..2474
                     /locus_tag="JN334_003205"
     CDS             1842..2474
                     /locus_tag="JN334_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAY7439253.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLRVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            2471..2875
                     /locus_tag="JN334_003206"
     CDS             2471..2875
                     /locus_tag="JN334_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439254.1"
                     /translation="MIRIAALLSILLTTSANSECWIVTNLHGYGAMNGGRYGFTKDST
                     EDSVFHVTINGDKSSVYESVSGVYPEMKYTALSSNTMVGEYQSGGGITVETWSITTDK
                     KALYSKVMNIPGMQQLTSTKSFVGDVVGTCNQ"
     gene            3336..3662
                     /locus_tag="JN334_003207"
     CDS             3336..3662
                     /locus_tag="JN334_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7439255.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRIERKNQRTWYSKPRSEMGVTCVGRQKMKL
                     GSKPLI"
     gene            3674..4297
                     /locus_tag="JN334_003208"
     CDS             3674..4297
                     /locus_tag="JN334_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439256.1"
                     /translation="MSSIRLTTRMKEEIARNALIKSGVFTELEEVTKLKNQLALDARV
                     IAFGGKKKTEEVDRLSSKLAAISEELEKMGCSFYSCDVRSTSIYLTVSGRRVGWHSYG
                     KDGNGEDILLPTPEKDKCMFDAEHEITKRFDEICALQQKLEAKKKDIESNVWAALNSV
                     TTVKRLIEVWPESKELLPKEADKASTALPALRVEDLNKMIGLPSEVA"
     gene            4335..4631
                     /locus_tag="JN334_003209"
     CDS             4335..4631
                     /locus_tag="JN334_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439257.1"
                     /translation="MEFKGTKGKWEIMMDGDEIKIIQADSLENGAGWRSYIAICEEVQ
                     CIEDANLIAAAPDLLEALQLLLKQTKNRTKTTYPEWYGAVNKGLAAIRKALGEE"
     gene            5400..6005
                     /locus_tag="JN334_003210"
     CDS             5400..6005
                     /locus_tag="JN334_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HAY7439258.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAAMQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
     gene            6005..6388
                     /locus_tag="JN334_003211"
     CDS             6005..6388
                     /locus_tag="JN334_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439259.1"
                     /translation="MAHSITVRLNKPAREFQAGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVVFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSINLELLNAKIGFA
                     TSGNSQQQQSSNHQNHPEYDDSIPF"
     gene            6412..6705
                     /locus_tag="JN334_003212"
     CDS             6412..6705
                     /locus_tag="JN334_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HAY7439260.1"
                     /translation="MPAPLYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKKEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            6716..6880
                     /locus_tag="JN334_003213"
     CDS             6716..6880
                     /locus_tag="JN334_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HAY7439261.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMLRSGKK"
     gene            6877..7416
                     /locus_tag="JN334_003214"
     CDS             6877..7416
                     /locus_tag="JN334_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439262.1"
                     /translation="MKQMSLIEMDGFLKGKCIPRDLKVNETNAEYLVRKFGELESKLE
                     TALRECRSAGITIDNLEAKCEALAAENARLKSAVTQQIELRAEIKKAGRPPHADFWVQ
                     SICEAEEKVKRALEETQATDAFLAEVLAQGVEMYADNLDNGADDAERGGFDYAVKFLR
                     SEASSVRLFADQLRKGGNQ"
     gene            7413..7712
                     /locus_tag="JN334_003215"
     CDS             7413..7712
                     /locus_tag="JN334_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439263.1"
                     /translation="MSVYLIDKRRRGQQIPPVGIPNHTWFCVLDIDGMDSLVDTRHYC
                     DTATATPAKAKKMADLIENWTPPDGWCNGNDRDWHEKMKGYICDFLRKCNGFRVM"
     gene            7714..8433
                     /locus_tag="JN334_003216"
     CDS             7714..8433
                     /locus_tag="JN334_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY7439264.1"
                     /translation="MSKIDYQALREKAEKATCGVWSLEYGEGRFDGDDALIHREVVGY
                     LPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLALLGELETAKKRIAELEAE
                     PVSQAYNLPELIEGMEVFIDVSTCDADLGNRYFGTVTEASELDTAKNGYILLVQDAEP
                     NFDINGNSPVTPGGWISCSDRMPEDTKMLLAFSQGEIVAAYWNWVVNPIDYKKYRAFT
                     YLSGYILDDVTHWMPLPEPPL"
     gene            8530..8709
                     /locus_tag="JN334_003217"
     CDS             8530..8709
                     /locus_tag="JN334_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eag protein"
                     /protein_id="HAY7439265.1"
                     /translation="MSCPKCGSGNIAKEKTMRGWSDDYVCCDCGYNDSKDAFGERGKN
                     DFVKINKEREGNEKS"
     gene            8810..9154
                     /locus_tag="JN334_003218"
     CDS             8810..9154
                     /locus_tag="JN334_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439266.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVDLLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            9245..9592
                     /locus_tag="JN334_003219"
     CDS             9245..9592
                     /locus_tag="JN334_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7439267.1"
                     /translation="MEEEIFTREEAASYLKVDKGTITQWIRSGRLQAAKINPDKPKSP
                     YRICKSDCIAALKSVRHNSAVNAVDVQEVKACQSNYAVARGTAISSRQMDQELDALLK
                     HRTKGKRKNFTIV"
     gene            9469..>10360
                     /locus_tag="JN334_003220"
     CDS             9469..>10360
                     /locus_tag="JN334_003220"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7439268.1"
                     /translation="MSIKLRGGTWHCDFVAPDGSRVRRSLETSDKRQAQELHDRLKAE
                     AWRVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWMLHFREAILSDITTEK
                     IMEAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTKTRHLAILRAILNMA
                     VEWGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPVVMFAITTGLRRSNV
                     TDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQGLHKRWVFVHTKPA
                     YRSDGTKTAAVRKMRTDSNKA"
BASE COUNT         3091 a         2198 c         2475 g         2596 t
ORIGIN      
        1 atttttaaac cagaaatcga caaacgaaat acttaaccag ccgtggttat aaccagcgac
       61 cagtagcgat ttgatttttg atttcatggt tcacctgtcg aaaaacacgt agccagtttt
      121 cgatacggtg attgcggatg atggtttgga ttgtggttga atagtttctg gcttctcgtc
      181 gttccagcgc tgaccgttca ggtagctcga tggtaacaac ctgtcgaatc cgaactgctt
      241 accattcctg catgcgatgt cttctgccag catcgtggca aattcgcttg ccgtacccct
      301 ggtagtttta cgccattccc tgaactgtgt tctgaatgcc gaagctgcgt ttttcttccc
      361 ggctttccgc atgcctgcac accagaatat ttcctcgaat gccttgtcgg tttcttcgtg
      421 acggtcatgt gatttttcac actccgtccg aacactttcg gacatagtgt ttttattatt
      481 tcttttttct tttgtaatag tttcttttgt gtgtccctgt tttggtgaca gcgctgtcac
      541 cgttttggtg acactttttg tcaccaatgc agtgacatta tcaccagagt agtgacaccc
      601 ttctatttgc cattcctcga tgttcttgtt aggcccgatt tgctggcctt cgcgaaggat
      661 aaccttcatc gcgataagct cattcttggc cttgtttacc ttctgtcttg gcagcctggt
      721 aatttgagct aactgactat cagagatgcg atccatcttt ttaccgtagc cgtatgtttt
      781 acggcatatg gcgtgggcaa ccttgctctg atttttcgtt aaatctgcgc cgataagctc
      841 ttcatacagg gcatttgcaa gacgggtata accatcttca acttctgcca cacgacgctc
      901 cacaggccgt tgtgaaggcc ttaaatgtgt tacggttgca agattactca tgacctttct
      961 ccttctgcat cagcttcact ttttccaact cagcccggaa tcgaccaggc tgcttgaagc
     1021 tggacaggaa gcgatcacgt agtatgtgtt tgtgaatttt gtcctggtaa ggactgagtt
     1081 gttttgtcat aattactccc gtggattgat ccagtctttc tacattaggc ctcgaagaat
     1141 tcgccgttct tcggggcttt ttcttttgtc aggtaatcgg caagccgctt agtcaattca
     1201 gccatttcat cgtcttcgat tccgtattcc agaacagcaa gcatcatgct tacctgcgag
     1261 aagaaaccat tcttccatcg gcttacctga tattcaggaa cccccattgc tcgagcgaat
     1321 gtcttctgcc ccatcagtgc cagtttgttc agcaaggctg actcgatgcg agccgctttc
     1381 ttgcttttag ttgcaatagt acccatagat aatttcctta atgattagat agagttggct
     1441 tcgcaaagaa acgcaaaacc atagagattt gtttctggta atgccctttt tcagggctgg
     1501 gatgtgtaag agcgggaatg tcttaagcgg ctttaccgcg tttagttccg tactgtaacc
     1561 aaaccggatc acagttaagc gccatagcaa tctcaaacaa gaagcgcggt cgcttggtta
     1621 ctccagcttc aatcagttga attgattgct gtttaacacc ggctttggtt gccagttcgg
     1681 tttgcgtcat ttttaacgca attcgcctct tcttgaggcg ttcagaaaga gtttgcatat
     1741 cgcctccatc aacaaacttt cttgtatttt catacaatgt atcttgtttg tcaaatacag
     1801 tttttcttgt gaagattgga ggtaaataac agaggtggct tatgagtatt tcttccaggg
     1861 taaaaagcaa aagaattcag cttggactta accaggctga acttgctcaa aaggtgggga
     1921 ctacccagca gtctatagag cagctcgaaa acggtaaaac taagcgacca cgctttttac
     1981 cagaacttgc gtcagctctt ggcgtaagtg ttgactggct gctcaatggc acctctgatt
     2041 cgaatgttag atttgttggg cacgttgagc ccaaagggaa atatccattg attagcatgg
     2101 ttagagctgg ttcgtggtgt gaagcttgtg aaccctacga tatcaaggac attgatgaat
     2161 ggtatgacag tgacgttaac ttattaggcg atggattctg gctgagggtt gaaggtgatt
     2221 ccatgacctc acctgtaggt caaagcatcc ctgaaggtca tatggtgtta gtagatactg
     2281 gacgcgagcc agtgaatgga agccttgttg tagccaaact gactgacgcg aacgaagcaa
     2341 cattcaagaa actggttata gatggcggtc agaagtacct gaaaggcctg aatccttcat
     2401 ggcctatgac tcctatcaac gggaactgca agattatcgg tgttgtcgtg gaagcgaggg
     2461 taaaattcgt atgatcagga ttgcggcgct actctcaata ctcttaacta ccagcgccaa
     2521 ttctgaatgc tggattgtca caaacctgca cgggtacggg gcaatgaatg gcggtcgtta
     2581 cgggtttaca aaagacagca cggaagattc cgtttttcac gtaacaataa atggcgataa
     2641 atcatcagtt tatgaatcag tctctggcgt ctatccagag atgaaataca ctgctttgtc
     2701 atcgaacact atggtaggag aataccagtc tggaggagga ataaccgttg aaacttggtc
     2761 aatcactaca gacaaaaaag ctctttactc caaagtaatg aatatcccag gtatgcaaca
     2821 acttacatca accaaatcat ttgttggtga tgtagtcgga acctgcaacc agtaatcccc
     2881 acctcaatct cgataaccaa aaacaaacta tttttcattt aaaaacaatg gagtttgttt
     2941 ttcacgcccc tttttacaat atttcttgtt tacaacatac aatctttctt gtaattttaa
     3001 gccatcagca ggacgcactg accaccatga aggtgaggct cttaaaaatt aagccctgaa
     3061 gaagggcagc attcaaagca gaaggctttg gggtgtggtg aagccagcta gtcactggca
     3121 agtgcttacc tactgttgag cggtgaagcg ctcccaacgc tagcaatagc gtggacgaga
     3181 tggggagccg cgggcgataa ggccgccata acgcgcacgt tgtcgcatgg aaaaatcgct
     3241 ggggtgccgg ttatacccct ccgaatgaga ctcaacaagc tggagctaga ctaccagcca
     3301 ccacaccacc aaagctaact gacaggagaa tccagatgga tgcacaaaca cgccgccgcg
     3361 aacgtcgcgc agagaaacag gctcaatgga aagcagcaaa tccactgttg gttggggtaa
     3421 gcgcaaaacc agttaaccgc cctattctct cgctgaatcg caaaccgaaa tcacgagtag
     3481 aaagcgcact aaatccgata gaccttacag tgctggctga ataccacgaa cagattgaaa
     3541 gcaacctgca gcgtattgag cgcaagaatc agcgcacatg gtacagcaag ccacgcagtg
     3601 aaatgggggt gacttgtgtt ggtcgccaga aaatgaaatt aggcagcaaa ccacttattt
     3661 gagaggaatt aatatgtcat caatccgctt aactacgaga atgaaagagg aaatcgctcg
     3721 taacgcttta attaagtctg gggttttcac tgaacttgaa gaagtaacaa agttaaagaa
     3781 ccagcttgca cttgacgcca gagttattgc gtttggcggt aaaaagaaaa ctgaggaagt
     3841 ggatcggtta tcatccaagt tagcagctat aagtgaagaa cttgagaaga tgggatgttc
     3901 attttactca tgcgatgttc gttctacttc gatttatctg actgtatctg gaagaagggt
     3961 tggctggcat tcatacggaa aagacggcaa cggtgaagat atattgctcc ccaccccaga
     4021 aaaagataaa tgcatgtttg acgcagaaca cgaaataaca aaaaggtttg atgaaatctg
     4081 cgcattgcaa caaaaacttg aagccaagaa aaaggatatc gaatcaaatg tatgggctgc
     4141 tttgaactca gtcacaacag ttaagcgact tattgaagtt tggcctgaaa gcaaagaatt
     4201 gctaccaaaa gaagcagata aagcaagtac agcacttcct gctttacggg tagaagattt
     4261 gaataagatg attggacttc cttccgaggt cgcatagtcg gcctttattt ttggcataaa
     4321 caacagaggc taacatggaa tttaaaggta ctaaaggtaa gtgggaaata atgatggatg
     4381 gcgatgagat taaaatcatc caggcagact cacttgaaaa tggcgcaggc tggcgttcgt
     4441 atattgcaat ctgtgaggaa gttcaatgca ttgaagatgc caatctaata gcggcagcac
     4501 ctgaccttct cgaagcactt cagttattac ttaagcaaac caaaaataga acaaagacaa
     4561 catatccaga atggtatgga gctgttaata aaggtcttgc agcaatcaga aaagctcttg
     4621 gggaagaatg atgaataaga aatacattgt tgaagttata gagcgagaaa cgaaagaagt
     4681 aattaaacat ttcgaatttg ataattatag aaaagctgac cgcgtagaag aaggattgtt
     4741 gcgacaaagt aatctcgaaa aatttgatgt tgtcatgcga tgcgaataag cacctatagc
     4801 agatttgcga gtctgctatg tgagcaatgt cgctcgtaac taaacaggag ccgacttgtt
     4861 ctgattattg gaaatcttct ttgccctcca gtgtgagggc ttttttatat gcataccaat
     4921 aacgcttcac tcgaggcgtt ttcgttatgc aatcaaacag aaggagcatc ctatgcaaca
     4981 gttcgctatt gcaggggcgg catcggttcg ccctttcaac ccgattttat cggtacagca
     5041 ttcacgaaaa aacattctaa ccggagcaga ctttaaacaa ccaagaatga aaagtttgct
     5101 cgaaaagctt tgggatattt tgaaacaaca aggccgccca tgagttttac agataactgg
     5161 tcagacgaag aattcattcg tcagatgaaa gaattaatcg gtaacgaagg agatattcat
     5221 gtcacttgca accacagtga aggagagcaa gttacagaga cgcatgtaca cgcagaaagc
     5281 tctctggtat cgccataatg gcgaccgcga aggaatgcgg gtatgcctta atttgtcccg
     5341 agtcgaagta ttaaatcagc gttatttcct tgggccatgt ccattctgag aacaaacata
     5401 tgagcaaaga attttacgca agactggcag ccattcagga gaatctgaac gcgccaaaga
     5461 atcagtacaa ctcattcggc aaatataaat acagaagctg cgaagacatt cttgagggtg
     5521 ttaagccgtt actgaatggc ctgtttttat caatcagcga tgaagttgtg ttgattggtg
     5581 atcggtatta cgtgaaagcc acggcaacta ttactgatgg cgaaaacagt catacagcaa
     5641 ccgctcttgc acgagaggaa gaaagcaaga aaggaatgga ttctgcacaa gttacgggag
     5701 ccacaagctc ttatgcacgc aagtattgcc tcaatggttt gttcggcatt gatgatgcga
     5761 aagatgcaga tacagacgag cataaacatc agcagaacgc agcagcaaag caatcaaaac
     5821 catcacctac acctgaacag gttctaaaag cattcactga cgcagcaatg cagaaaaaca
     5881 ccgtagaaga gcttaaacag gcgttcgcca aagcgtggaa gatgctcgaa ggcacaccgg
     5941 agcagcacaa agcgcaggac gtttacaaca tcagacgaga cgaattagaa ggagcggctg
     6001 cttaatggca cattcgatta ctgtaagact aaacaagccc gcaagagagt ttcaggccgg
     6061 ggaaaatatc ggattcaaca tccgtgctgg cgttcagtat tacgatcgcc agacaaaaaa
     6121 gaaagaatgg acaaactaca gcgccgttgt atttgccaag ccgggagcgc aagcggatta
     6181 ctaccgtagt gttcttgttg aaggtggcat tgttgaaatt accggagaaa acatcagggt
     6241 tgatgtttat caggggcaaa atggtcaatc aatcaatctt gaattactga atgcaaagat
     6301 tggatttgca acttcaggaa acagccaaca gcagcaaagt agcaatcatc aaaatcatcc
     6361 tgaatacgac gattcaattc ccttctagat ttgcaaaata aggattccat tatgccagcg
     6421 cctctgtatg gtgcggatga cccgcgccgc tgttccggca attccgtatc ggaggtgctg
     6481 gataaattca gaaaaaacta cgatcggata atgtcgctac cgcaggaaac gaaaaaggaa
     6541 aaggaatttc gccactgtat atggcttgca gagaaagaag aacgcgagcg aatttaccag
     6601 acatcaatcc gaccattccg caaagccaca tatacccact tccctgaaat tgacccgcgc
     6661 ctgcgtaatt accgctcacg ctatggcgct atcagtaatg actgaggaat ttaccatgag
     6721 aggacttgca tacaatcccg gcattcttcc ggcagaaatg attattcgcc aacgcgtaaa
     6781 gccaatgcca tcgagagagg aattgcttaa gagaaattct tttccatcag ttaatcaaaa
     6841 caaatatctg aatgcgatgt tgcggagtgg gaagaaatga aacaaatgtc actaattgag
     6901 atggatggtt ttctgaaagg taaatgcatc ccccgagatt taaaggttaa cgaaacaaac
     6961 gctgaatatc tggtgcgtaa gttcggtgaa cttgaatcaa aactagaaac ggcgttgcgg
     7021 gagtgtcgtt ctgctggaat cacgattgat aaccttgaag ccaagtgcga ggcgctggcg
     7081 gcggagaatg cgaggctgaa atctgcggta actcagcaaa ttgagcttcg tgcggagata
     7141 aaaaaggcag gcaggccacc tcacgctgac ttctgggttc agtcaatttg cgaagctgag
     7201 gaaaaggtta agcgagcgct tgaagaaacc caagccaccg atgctttcct ggctgaagta
     7261 ctggcgcagg gggtagagat gtacgcagat aacctcgaca acggagcaga cgacgcagaa
     7321 cgaggtggtt ttgattatgc cgttaagttt ctacgcagtg aagcgtctag tgtacgtttg
     7381 ttcgccgacc agcttcgcaa aggaggcaac cagtgagtgt atatcttatt gataaacgtc
     7441 gacgtgggca acaaatacca cctgtaggaa ttccgaatca cacatggttt tgcgtacttg
     7501 atatcgatgg tatggattcg ttggttgaca ctcgtcatta ctgcgatacc gcaacagcta
     7561 ctccggcgaa agcaaagaaa atggctgatc tgatagaaaa ctggactcca cctgatggtt
     7621 ggtgcaatgg gaatgatcga gattggcatg aaaaaatgaa gggctacatc tgcgatttct
     7681 tacgtaaatg caacggattc agggtgatgt gacatgagca agattgacta tcaggcactg
     7741 cgtgaaaagg cagagaaagc aacgtgtggt gtgtggtcgc tcgaatatgg agagggccga
     7801 tttgatggtg atgatgcgct aattcatcgt gaagttgttg gatatcttcc catttgcaga
     7861 attgaaggag cgcatcctga aagcggtttc gatgaagatt tccaaatgga acagcaggcc
     7921 aatgctgaat tcatcgctgc agccaatccg gctaccgtct tggcgctgct gggcgagctg
     7981 gaaacagcaa aaaagcgcat agcagaactg gaagccgaac ctgtaagcca agcttacaac
     8041 ttgccagaat taatcgaagg catggaagtt ttcattgatg taagtacttg tgatgctgat
     8101 ttaggtaatc gctatttcgg caccgtcacc gaggcgtcgg aacttgatac tgccaagaat
     8161 ggttacatcc tcctagttca ggacgcagag ccaaacttcg atataaatgg caactctccg
     8221 gtaactccgg gtggttggat aagctgtagt gatcgaatgc cggaagacac caaaatgtta
     8281 ctggcattta gtcaaggtga aatcgtggcc gcatattgga actgggttgt aaatccaatt
     8341 gattacaaaa aatatagagc tttcacgtat ttatcaggat atatcttgga tgacgtaact
     8401 cactggatgc cgctaccaga gcctccactt tgaaagcgaa gcttatacat atcttttaca
     8461 tcagcaatct attgttaatc tccaatcaat gttacgttgt catctcactc atgctttgga
     8521 ggtagtgata tgtcttgtcc aaaatgcggt tctggaaata ttgcaaaaga aaaaacaatg
     8581 cgtggatggt ctgatgatta tgtgtgctgc gattgcggat acaacgactc taaagacgca
     8641 tttggagagc gtggtaaaaa cgattttgtc aaaattaata aagaacgcga aggcaacgaa
     8701 aaaagctaat ttatttattc atatatgaaa acaatgtaac caatattcga attgaagaac
     8761 tgaaagaaca ccatgccgcc tgatggcggt tttttattgg agacaagaaa tgtcagattt
     8821 ggctatgaag gttttgaaat ggcaatcgac tggcgatgtt ggcatcagta gcgcaactct
     8881 tgcctcaatc gcatgtggac tgaaaaagaa tatctatggt catcacttcg gcgctcccca
     8941 tgacgcagcc gatttcagac gatgcgttgc acttgttgag cagatcccag aaatcagaga
     9001 ttcattcgac aaggttgcaa agcgcgttcc ggcattcaaa ggcatcctca acgaatggga
     9061 ttccctcgtt gatctgttga agtctgaaat gaagatacac ggaaacaaag caccagagac
     9121 ttacagaaga attagcgagt tacgcaagga ctaaccacag cctcatactc gatgaggcct
     9181 gttcattgct caatgatatc cagacctacc gcaataatac caatccaata aatggagatt
     9241 ccaggtggaa gaagaaatct tcactcgtga agaggcagcg tcgtatctga aggtagacaa
     9301 aggcactatc acgcagtgga tacgaagtgg acgacttcag gccgcaaaga taaatccaga
     9361 taaacctaaa agcccatatc gcatttgcaa gtcagactgc attgcggcgc ttaagtctgt
     9421 gagacacaat agcgcggtga atgcggttga tgtgcaggag gttaaagcat gtcaatcaaa
     9481 ctacgcggtg gcacgtggca ctgcgatttc gtcgcgccag atggatcaag agttagacgc
     9541 tctcttgaaa catcggacaa aaggcaagcg caagaacttc acgatcgtct gaaagcagaa
     9601 gcgtggagag taaaaaatct cggggaatca ccgaaaaagc tattcaagga agcctgcata
     9661 cggtggctgc gtgagaaatc ggataagaag tccattgatg atgacaagag cattatatcg
     9721 ttctggatgt tgcacttcag agaagccatt ctctctgaca taacaacaga aaaaataatg
     9781 gaggcggtag acgggatgga aaaccgccgc catcgcctga actgggagat gagccgggac
     9841 aggtgtttgc gacttggcaa tccggtgccg gagtataaac caaagcttgc aagcaaagga
     9901 acgaagacga ggcatctggc aatacttcgc gccattctca atatggctgt tgaatgggga
     9961 tggcttgaca gggcacccaa aatatcaaca ccacgcgtta agaatggacg catcagatgg
    10021 cttacagagg aggaatcgaa gcgcctgttt gcagaaattg ctcctcactt cttccctgtg
    10081 gtcatgtttg caatcacgac aggccttcgc cgttccaacg ttacagacct tgagtggtca
    10141 caggtcgatc tggataagaa aatggcatgg atgcaccctg atgaaacaaa agctggcaat
    10201 gcgatcggag ttcctcttaa cgaaaccgca tgccagatat taagaaaaca gcaggggctc
    10261 cataagagat gggtatttgt ccacaccaaa cctgcctacc gaagcgacgg aacaaaaaca
    10321 gcagcggtaa ggaagatgag aaccgacagc aacaaggcat
//
LOCUS       DADLTY010000148        10285 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.148, whole genome shotgun sequence.
ACCESSION   DADLTY010000148 DADLTY010000000
VERSION     DADLTY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10285
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.148"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(33..3041)
                     /locus_tag="JN334_003221"
     CDS             complement(33..3041)
                     /locus_tag="JN334_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAY7439269.1"
                     /translation="MPVDFLTTEQELNYGRYVAEPNDVQLARYFHLDERDLAFINQRR
                     GRHNRLGIALQLTTARFLGTFLTDLTRVLPGVQQFVAVQLNIRRPEVLTRYAERVTTL
                     REHTALIKEYYGYHEFGDFPWSFRLKRLLYTRAWLSNERPGLMFDFATAWLLQNKVLL
                     PGATTLVRLVSEIRERANQRLWKKLAALPDSWQTARVTELLDIPEGQRTSPLEQLKKG
                     PVTVSGPAFTEALERYIRLRNLEFSRLNFTGLPAIQLRNLARYAGMASVKYIARMPQQ
                     RKLAVLTAFVKAQETAALDEAVDVLDMLILDITRAAKKTGQKKRLRTLKDLDRAALIL
                     ARACSLLLDEQADDAELRETIFNSIPKSRLAESVSKVNELARPQNNNFHDEMVEQYGR
                     VKRFLPAVLRDLHFQAAPAGEHTLSAIHYLTELNGSKKRILDNAPEHIITGPWKRLVY
                     DAEGRIQRAGYSLCLLERLQDALRRRDIWLENSDRWGNPREKLLQGEEWQAQRVPVCR
                     ALGHPTDGHKGVQQLAVQLDETWKTVASRFEGNAEVHICHDGKHPSLTISSLEKLEEP
                     TSLHRLNSRVRLLLPPVDLTELLLEIDARTGFTREFTHVSESGARAQDLHISLCAVLM
                     AEACNIGLEPLIKHNIPALTRHRLSWVKQNYLRAETLVSANARLVDFQSTLELAGRWG
                     GGEVASADGMRFVTPVKTVNSGPNRKYFGSGRGITWYNFVSDQYSGFHGIVVPGTLRD
                     SIFVLEGLLEQQTGLNPVEIMTDTAGTSDIIFGLFWLLGYQFSPRLADAGEAVFWRVD
                     KSANYGALDELARGCADLSKAEDQWDEMMRTAGSLKLGTIHASELIRSLLKSSRPSGL
                     AQAIMEVGRVNKTLYLLNYIDDEDYRRRILTQLNRGEGRHAVARAICYGQRGEIRKRY
                     REGQEDQLGALGLVTNAVVLWNTLYMQEALSWIRSNGEEIGVEDIARLSPLMHGHINM
                     LGHYTFTLPEDILKGELRELNFNLNNELTS"
     gene            3205..3762
                     /locus_tag="JN334_003222"
     CDS             3205..3762
                     /locus_tag="JN334_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7439270.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            3945..4805
                     /locus_tag="JN334_003223"
     CDS             3945..4805
                     /locus_tag="JN334_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HAY7439271.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            5015..5554
                     /locus_tag="JN334_003224"
     CDS             5015..5554
                     /locus_tag="JN334_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7439272.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFGVVCGKGRNPTLRLWPAIFSGEIDV
                     FPGDRRSRLISSMTSVAPA"
     gene            complement(5526..6362)
                     /locus_tag="JN334_003225"
     CDS             complement(5526..6362)
                     /locus_tag="JN334_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HAY7439273.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(6362..7165)
                     /locus_tag="JN334_003226"
                     /note="aph(3'')-Ib"
     CDS             complement(6362..7165)
                     /locus_tag="JN334_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAY7439274.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(7226..8041)
                     /gene="sul2"
                     /locus_tag="JN334_003227"
     CDS             complement(7226..8041)
                     /gene="sul2"
                     /locus_tag="JN334_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAY7439275.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            8065..8142
                     /locus_tag="JN334_003228"
                     /pseudo
     CDS             8065..8142
                     /locus_tag="JN334_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            8371..8547
                     /locus_tag="JN334_003229"
     CDS             8371..8547
                     /locus_tag="JN334_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HAY7439276.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            complement(8729..9733)
                     /locus_tag="JN334_003230"
     CDS             complement(8729..9733)
                     /locus_tag="JN334_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HAY7439277.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLAT
                     CPATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISR
                     LSTLLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCV
                     GTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLL
                     VQCARVFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA
                     "
     gene            complement(9812..>10285)
                     /locus_tag="JN334_003231"
     CDS             complement(9812..>10285)
                     /locus_tag="JN334_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021138877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7439278.1"
                     /translation="LMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRV
                     HAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAVVLWNTVYLERA
                     AHALRGNGHAVDDALLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT         2445 a         2753 c         2702 g         2385 t
ORIGIN      
        1 gggtttgagg gccaatggaa cgaaaacgta cgctaagaag ttaattcatt gtttaaattg
       61 aaatttagtt ctctcagttc ccccttcaaa atatcctccg gtagcgtaaa cgtataatgc
      121 cccagcatat tgatatgccc gtgcatcagt ggggacaacc gggcgatatc ttcaaccccg
      181 atttcttctc cattactgcg tatccagctc agggcttcct gcatataaag tgtgttccac
      241 agtaccactg cgttagtgac caggcccagt gcccccagct gatcttcctg accttcacga
      301 tagcgctttc tgatctcacc gcgctgcccg tagcagatcg ccctcgccac agcatggcgg
      361 ccttcccccc ggtttagctg cgtcaggatc cgccgacgat aatcctcatc atcaatataa
      421 ttaagaaggt acagcgtctt gttgacgcgc cccacttcca tgatcgcctg tgccagccct
      481 gatgggcgcg agcttttcag caaagagcga atgagttctg aagcatgaat ggtgcccagt
      541 ttcagcgaac cggcggttcg catcatctca tcccactgat cctcggcttt cgacagatcg
      601 gcacaaccac gtgccagttc gtccagtgca ccgtaatttg ccgatttatc cactcgccag
      661 aataccgctt caccggcatc ggcaagccgg ggggaaaact ggtatcccag cagccagaag
      721 aggccaaaaa taatgtcgct ggtaccggct gtgtctgtca tgatctcaac cggattcagc
      781 cctgtctgct gctccagaag gccttccagc acaaaaatgg aatctcgtaa tgtgccgggg
      841 acaacgatgc cgtggaatcc agagtactga tcagagacga agttgtacca ggtgatgcca
      901 cgtccggaac caaaatattt tctgttaggt cctgaattga cggttttcac cggcgtgaca
      961 aagcgcatgc catcagctga agccacttcg ccaccacccc agcggccagc aagctccagt
     1021 gtggactgaa aatcaaccag gcgggcattg gcgctgacca gcgtttctgc ccggaggtaa
     1081 ttctgtttta cccagctaag ccggtggcgc gtcagcgccg gtatattgtg ctttatcagc
     1141 ggttccagcc cgatattgca ggcctctgcc atcaggaccg cgcacaggct gatgtgcaga
     1201 tcctgtgccc gggccccgga ttcactgaca tgcgtaaact cacgtgtaaa tcccgttctg
     1261 gcatctattt caagcaacag ttccgtcaaa tctactggcg ggagtagcag ccttacccga
     1321 ctgttgagac gatgcaacga tgttggctcc tccagtttct ccaggctgct gatagtcagg
     1381 gaaggatgtt taccgtcatg gcaaatatga acctccgcat ttccttcaaa gcgggatgcg
     1441 acggttttcc aggtttcatc tagctggacc gccaactgtt gcacgccttt atgtccatca
     1501 gtgggatgtc ccagcgcccg gcaaacgggg acccgctgag cctgccattc ttccccctgc
     1561 aacagcttct cgcggggatt tccccagcga tcgctgtttt cgagccagat gtcccttcga
     1621 cgtagtgcat cctgaaggcg ctccagcaga caaagcgagt aaccggcacg ctgtatccgg
     1681 ccctccgcat cgtataccag gcgtttccag gggccggtaa taatatgttc aggcgcattg
     1741 tccaggatgc gctttttcga gccgttcagt tcggtcagat aatgaatggc ggacaacgta
     1801 tgttctccgg ctggcgctgc ctggaaatgc aggtcgcgca ataccgccgg aagaaagcgc
     1861 tttacccgcc cgtactgctc caccatttcg tcatggaaat tgttgttctg aggccgggcc
     1921 agctcattta ccttgctgac ggattctgcc agcctgcttt tcggtatgct gttgaatatg
     1981 gtctccctca gttcagcatc gtcagcctgt tcatccagca acaatgaaca tgcccgcgcc
     2041 aatattagcg cagcacgatc aagatcttta agcgtcctga gccgtttttt ctgccccgtt
     2101 ttcttcgccg cacgagtgat atccagtatc agcatatcga gcacatcaac ggcctcatcc
     2161 agcgccgcag tctcctgcgc tttaacgaat gctgtgagta cggccagttt tctctgctgt
     2221 ggcattcgcg cgatatattt taccgacgcc atgcctgcgt aacgggccaa attacgcagt
     2281 tgtatagcag gcagaccggt aaaattcagt cgggaaaatt ccaggtttcg cagccggata
     2341 taccgctcca gcgcttcggt aaatgccggg ccgctgacgg tgaccggtcc ctttttcagt
     2401 tgctccagcg gtgaagtacg ctgcccctca ggaatgtcca gaagctccgt cacgcgggcg
     2461 gtctgccagc tgtccggcaa cgcggccagt ttcttccaca gccgctgatt tgcccgttcg
     2521 cgtatttcac tgacgagacg tacaagtgtg gttgctccgg gcagcaggac cttattctga
     2581 agcagccacg cggtggcaaa atcaaacata aggccgggtc gctcattact gagccatgcg
     2641 cgggtgtaca gcaggcgctt caggcggaaa gaccagggga aatcaccaaa ttcatggtaa
     2701 ccgtaatatt ccttaatcag cgcagtatgc tctctgaggg tggtaacccg ttctgcgtag
     2761 cgggtaagga cttccggacg gcgaatatta agctgcaccg cgacaaattg ctgaacacct
     2821 ggcagaaccc gggttaagtc tgtcagaaag gtgcccagaa aacgggcggt ggtgagctga
     2881 agcgcaattc ccagccggtt atgccttccc cgccgctggt taatgaaggc aagatcccgc
     2941 tcatcaagat gaaaatagcg cgccagctgc acgtcattgg gttctgcaac atagcgacca
     3001 taattcagtt cctgctcagt ggtcagaaaa tcgacgggca taccggcctc cctgcctgat
     3061 ggtcatgaat taacaatttc gcaaccgtcc gaaatattat aaattatcgg acatcgtaaa
     3121 actgttacat taatatgtct attaaatcgt aaatttgtaa taatagacat gagttgtccg
     3181 atattcgatt taaggtacat ttttatgcga ctttttggtt acgctcgggt ctcaaccagt
     3241 cagcagtctc ttgatcttca ggtcagagca ctcaaagacg caggtgtgaa agcaaaccgt
     3301 atatttaccg ataaggcatc cggcagttca acagaccggg aagggctgga tttgctgagg
     3361 atgaaggtgg aggaaggtga tgtcattctg gttaagaagc tcgaccgtct tggccgcgac
     3421 actgccgata tgatccaact gataaaggaa tttgacgctc agggcgtggc agtccggttc
     3481 attgatgacg ggatcagtac cgacggtgat atggggcaaa tggtggtcac catcctgtcg
     3541 gctgtggcac aggctgaacg ccggaggatc ctagaacgca cgaatgaggg ccgacaggaa
     3601 gcaaagctga aaggaatcaa atttggccgc aggcgtaccg tggacaggaa cgtcgtgctg
     3661 acgcttcatc agaagggcac tggtgcaacg gaaattgctc atcagctcag tattgcccgc
     3721 tccacggttt ataaaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     3781 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     3841 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3901 aataaccctg gtaaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3961 ttcgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4021 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4081 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4141 tgatgagcac ttttaaagtt ctgctatgtg gtgcggtatt atcccgtgtt gacgccgggc
     4201 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4261 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4321 ccatgagtga taacactgct gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4381 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4441 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     4501 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4561 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4621 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4681 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4741 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4801 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4861 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4921 gtgagttttc gttccactga gcgtcagacc cctaaaagaa ctttccgcta agcgatagac
     4981 tgtatgtaaa cacagtattg caaggacgcg gaacatgcct catgtggcgg ccaggacggc
     5041 cagccgggat cgggatactg gtcgttacca gagccaccga cccgagcaaa cccttctcta
     5101 tcagatcgtt gacgagtatt acccggcatt cgctgcgctt atggcagagc agggaaagga
     5161 attgccgggc tatgtgcaac gggaatttga agaatttctc caatgcgggc ggctggagca
     5221 tggctttcta cgggttcgct gcgagtcttg ccacgccgag cacctggtcg ctttcagctg
     5281 taagcgtcgc ggtttctgcc cgagctgtgg ggcgcggcgg atggccgaaa gtgccgcctt
     5341 gctggttgat gaagtactgc ctgaacaacc catgcgtcag tgggtgttga gcttcccgtt
     5401 tcagctgcgt ttcctgtttg gggtcgtttg cgggaagggg cggaatccta cgctaaggct
     5461 ttggccagcg atattctccg gtgagattga tgtgttccca ggggatagga gaagtcgctt
     5521 gatatctagt atgacgtctg tcgcacctgc ttgatcgcgg ccgcgatagc tagatcgcgt
     5581 tgctcctctt ctccatccgc gttccaagct gcggaaaggc acccataagc gtacgcctgg
     5641 tcgagcaggc gacgcggatc gacgtccagc gcacgagaga atgcgtccgc catctgtgca
     5701 atgcgtctag gatcgagaca aaggtcgtct ctgtcagccg gatcgtagaa catattggcg
     5761 gcgccaaagc ccacttcacc gaccagaccg acgggatcta tcaccagcca gccgcgactg
     5821 gagaacatga tgttttcatg atgcagatcg ccatgtagcc cacgcagttc cgaggcattg
     5881 ctcatcattt gatcggctat aatcgccgcg tggacgtagt cagtttgaca acctgcgttt
     5941 tgatcatcgc gcgcccgctg aaacaaagct gcaaagcgat cccggatcgg gagaagggca
     6001 gaaggcaggg gttcctcaga tgcggcatac agcttcgcca ttagttccgc tgcaatttcg
     6061 gtcgcctggt agtcgccgtg ctcggcaacg atgtgagaga gcattcgctc cccggcatat
     6121 tcgagcaaca tcagattgtt ctcacgaccg agcaaccgga ctgctcccct cccattgcgc
     6181 cataccagat agtcggcccc gcgcagttca tcagcaatgt cttctatagg tttcaatccc
     6241 ttgacgattg caggagtccc gtctggcaat gaaactttcc aaacgaggct ggaaaaggtg
     6301 tccgcaatga gaacaggttg cgaaacgtgc caatgagcag gaaaaacagg cggcatgaac
     6361 atcaacccca agtcagaggg tccaatcgca gatagaaggc aaggcgttcg cggtcggggg
     6421 cttcgatccc caatacattg aataggacag cgaaggcgcg ctctgcttca tctggcgctg
     6481 cccagttctc ttcggcgtta gcaatcatga gtgccaaatc ggcatagcga tctgctgttc
     6541 cgagccgccc aaggtcgatc agacccgtgc attgaagagt tttagggtcc accatgaagt
     6601 tcggcatgca gggatcacca tggcaaacaa ccatatcggt gcgctcttgg tcgagccgca
     6661 ccggtagctc tcgttcgaca cgagccaaaa gatcgagctg cggcgtactc ttgtcctcgt
     6721 ccggtaagaa gtcgggattg acggcattgc gggacaccac atcaacggcg cgtccgaaca
     6781 ttcgcgacag cctgcgctca aacggacatt gatcaaccga taggctgtga acagcgccaa
     6841 gttgctgccc cattgacggc cacgctttga gcaaatccgc tccagacaga tcagccgccg
     6901 gtactcccgg aattgccgtt atcaccaagc atgcaccctc ctgttcctcc tgccagttga
     6961 tgacctcggg gcaagccaca cctcgacctt tgagccaaat gaggcggtca cgctctccag
     7021 cgagctcacc gcggcgggaa gcaggtgcga ttttcgcgaa ggcatgcccg tcaccacgtc
     7081 gaaaaacaaa atcaccagat tctccgcctc tgacaggcaa ccagtcagaa tgcgattcac
     7141 caaaaaaaat attagttcga ttcaatggag gttccttcag ttttctgatg aagcgcgaat
     7201 atagagaaat atcccgaatg tgcagttaac gaattcttgc ggtttctttc agcgccgcca
     7261 ataccgccag cccgtcgcgc aaggggcgcg gctcgtgtgt gcggatgaag tcagctccac
     7321 ctgcggcggc ggcaagctct gcagcgagtg tcgcggcccc gacatccccc ggaccacggc
     7381 ctgtgagcgc gcgcagaaag gatttgcgcg aaacagacag aagcaccggc aaatcgaagc
     7441 gcagccgcaa ttcatcgaac cgcgccagca ccgagagcga ggtttcggga gcagccccca
     7501 gaaaaaaccc catgccggga tcaaggacaa ggcggttgcg tttgataccg gcacccgtca
     7561 gcgccgcgat gcgcgcgtca aagaacgccg caatgtgatc catgatgtcg ccagcgggtg
     7621 cctcgcgccg atctgcctgc ccgtcttgca ccgaatgcat aacgacgagt ttggcagatg
     7681 atttcgccaa ttgcggatag aacgcagcgt ctggaaaacc gcgaatatca ttgagatagg
     7741 ccacaccacg cgacaaggca taggcttgcg tcgcgggttg ataactgtcg agcgagacgg
     7801 gaatgccatc tgccttgagc gcgtccagca ccggcgcgat acgcgcgatt tctgtgtcgg
     7861 acgaaacagg cgcggcgtcg ggattgctgg atgccggacc gaggtcgatc acatctgccc
     7921 cctcggccat cagcttacgc gcctgcgcaa tggctgcgtc tggcgccaga taccggcctc
     7981 catcggagaa actgtccgag gttatgttga cgatgccgaa aatgatgagc gatttattca
     8041 tgggggcttc tataataata ataatcgagc atgagtctca tacggatgct cgggtcgaaa
     8101 gggaatcccc aggcgagtaa cctgtttgcg gtgctccatt agctgcagga gcagaatagc
     8161 atacatctgg aagcaaagcc aggaaagcgg cctatggagc tgtgcggcag cgctcagtag
     8221 gcaatttttc aaaatattgt taagcctttt ctgagcatgg tatttttcat ggtattacca
     8281 attagcagga aaataagcca ttgaatataa aagataaaaa tgtcttgttt acaatagagt
     8341 gggtatcgga gacgaagaag gacaaggtac atgctgaccg ataccaagct gcgcaatctc
     8401 aagcccaggg acaaactcta caaagtgaat gaccgggaag gtctctatgt gggggtcgcc
     8461 tcagaaaacg gaatctatgg tcactcccgt ttttgcaaca ccgattttga cgacaagttg
     8521 gcttgcttga atctatccgg cgtctgaatg ggattttatt cccgcgcctc gatgagttcc
     8581 gcgcctgatg aacctccaga aaatatacgg cttcaatgag cctttccgtt ttacaggttc
     8641 ctcaacaggc cggtgggccg ttagtatcat caatatcagt attcgcaaaa ccagatgaat
     8701 gattgtttaa actggtgtat ttctgccttt atgcttcgta agtttgctgt cgcgccgtca
     8761 gtgcccaggc tattctggcc agcttgtttg ccagagcaca ggtgacgaca aagttgcttt
     8821 tccgacacaa caactccctg acccagtcgg ccaacttgcc agactggtgt tccagttttt
     8881 gtatgaatac cctggcacac tgaaccaaca aagttcggat ctttttgttg ccccgcttgc
     8941 taatccctaa caatgtcgtc cgacctcccg tgctgtactg tcggggtacc agccctgttg
     9001 ccgccgcaaa gtcacggctg ctggcgtact gcttcccgtc gccaatctca gttgaaatag
     9061 tactggcagt cagcgttcca acgcagggaa tactcagcaa gcgctgtcca acctcatctt
     9121 cgtccaactt tcgtttcaac tgagattcca gatctttaat ctgctcaaca agatagtgat
     9181 aatgctgttg taatttcagc agtaactggc tgagataaag aggcaaacta ctgtcctcaa
     9241 gaagggtact cagtcgacta ataacggcag cacctcgcgg aacgctgata ccaaattcca
     9301 gcagaaaagc atgcatctga ttagttgttt tcaccttatc ctgaaccagg gattcacgga
     9361 cacgatgcag agctcgcatt gcctgctgag attcggttct gggctgcacg aaacgcatag
     9421 atggacgtga tgctgcttca cagatagctt cagcatcaac gaagtcattt ttgttgcttt
     9481 taacgaatgg gcggacaaat tgcggtgata tcagctttgg aaaatgccct aactcttcca
     9541 gcttgcgtgc cataaagtga gaaccgccac aggcttccat cgcgatggtt gttgccgggc
     9601 atgtcgccag aaattcgatt agctttggtc gggtgaattt tttacggtaa acggccttcc
     9661 cacgatgatc ctgacaatga atatggaaag agttcttacc cagatcgata ccaataagcg
     9721 caatgttttc catgatggtt ctccgaatga aagcctgtcc tcagcatagt actgggaagg
     9781 agggagtgac catctcatta aataaagcac gctaagccgg ttgcagcggt cgtagcggcc
     9841 tgaacttgcc cgcgccgatc ttggcgctgc tgcgccagag gtaatcgccg gtgaggttga
     9901 tgtgctccca accgagcggc gacaggtact gcaacagcgc gtcatcaacg gcatggccgt
     9961 tgccacgcag cgcgtgcgca gcccgttcca gatagaccgt gttccacaac acgacggcag
    10021 ccgttaccag attgaggccg ctagcccggt agcgctgctg ctcgaaactg cggtcgcgga
    10081 tttcacccag gcggttgaaa aacactgccc tggccagcgc attgcgcgcc tcgcccttgt
    10141 tcaggccggc atgcacgcgg cggcgcagtt ccacgctttg cagccagtcc aggatgaaca
    10201 gcgtgcgctc gatgcggccc agctcgcgga gcgccacggc caggccgttc tggcgtgggt
    10261 agctgccgag ctttcggagc atcag
//
LOCUS       DADLTY010000149        10105 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.149, whole genome shotgun sequence.
ACCESSION   DADLTY010000149 DADLTY010000000
VERSION     DADLTY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.149"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            123..254
                     /locus_tag="JN334_003232"
     CDS             123..254
                     /locus_tag="JN334_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HAY7439279.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            514..1509
                     /gene="pgl"
                     /locus_tag="JN334_003233"
     CDS             514..1509
                     /gene="pgl"
                     /locus_tag="JN334_003233"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAY7439280.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1550..2503)
                     /locus_tag="JN334_003234"
                     /pseudo
     CDS             complement(1550..2503)
                     /locus_tag="JN334_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2687..3739
                     /locus_tag="JN334_003235"
     CDS             2687..3739
                     /locus_tag="JN334_003235"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HAY7439281.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3815..5248
                     /locus_tag="JN334_003236"
     CDS             3815..5248
                     /locus_tag="JN334_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAY7439282.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5431..7611
                     /locus_tag="JN334_003237"
     CDS             5431..7611
                     /locus_tag="JN334_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HAY7439283.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7752..9035)
                     /locus_tag="JN334_003238"
     CDS             complement(7752..9035)
                     /locus_tag="JN334_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HAY7439284.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9170..10051)
                     /locus_tag="JN334_003239"
     CDS             complement(9170..10051)
                     /locus_tag="JN334_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7439285.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2452 a         2359 c         2552 g         2742 t
ORIGIN      
        1 tttttttctt taaaaaaaca gtacattcta ttttataatg actgtgtatt cccatggaag
       61 gagatcaccc aattctcccc cccgactttt gacgcagtgt cttgttaaca gggggattga
      121 ccatgcttat tggctatgta cgcgtatcaa caaatgacca gaataccgat ctacaacgta
      181 atgcgtttat ttgtgccgga agtgagttga ttgttgcgcg aatgggaagt tggccagaag
      241 ttgttatggc atagtgttta cggtcgttga tacaggtaaa caaaagcggg gagagtcata
      301 ctccctgaat agtcgcgaaa atatctgtgg cattgtcaaa ttcgtgtcgg tgactaatat
      361 tttagtaata tgccattcga aatggagtgc taacttattg atttaattga tgtgcgatat
      421 tgtattttat cgtgttttgt tgtctgtttt ttatccagta agtgttgtta tatgtatgaa
      481 agataaggga tttatcattt ttgaggtgtg ttcatgaagc aaacagttta tatcgccagc
      541 cctgagagcc agcaaattca cgtctggaac ctgaatcatg aaggcgcact gacgctgaca
      601 caggttgtcg atgtgccggg gcaggtgcag ccgatggtgg tcagcccgga caaacgttat
      661 ctctatgttg gtgttcgccc tgagtttcgc gtcctggcgt atcgtattgc cccggacgat
      721 ggcgcactga cctttgccgc agagtctgtg ctgccgggta gtccgacgca tatttccacc
      781 gatcaccagg ggcagtttgt ctttgtaggt tcttacaatg cgggtaacgt gagcgtaacg
      841 cgtctggaag atggcctgcc agtgggcgtc gtcgatgtag tcgaggggct ggacggttgc
      901 cattccgcca atatctcacc ggacaaccgt acgctgtggg ttccggcatt aaagcaggat
      961 cgcatttgcc tgtttacggt cagcgatgat ggtcatctgg tggcgcagga ccctgcggaa
     1021 gtgactaccg ttgaaggggc cggcccgcgt catatggtat tccatccaaa cgaacaatat
     1081 gcgtattgcg tcaatgagtt aaacagctca gtggatgtct gggaactgaa agatccgcac
     1141 ggcaatatcg aatgtgtcca gacgctggat atgatgccgg aaaacttctc cgacacccgt
     1201 tgggcggctg atattcatat caccccggat ggtcgccatt tatacgcctg cgaccgtacc
     1261 gccagtctga ttaccgtttt cagcgtttcg gaagatggca gcgtgttgag taaagaaggc
     1321 ttccagccaa cggaaaccca gccgcgcggc ttcaatgttg attacagcgg caagtatctg
     1381 attgccgctg ggcaaaaatc tcaccacatc tcggtatacg aaattgttgg cgagcagggg
     1441 ctactgcatg aaaaaggccg ctatgcggtc gggcagggac caatgtgggt ggtggttaac
     1501 gcacactaac cgctgattta accggcgcag tctctcctgc gccggtgtat taacttatct
     1561 cctgtaacgc atgtctctgg cgttcgacga tattggtcca caaattgtct ttatcgtcag
     1621 tccacaaatt aatcagcaag gaaaaaaagc gttcagctgc cggagaaagc acggcatctt
     1681 tgcggcgaat aatccccaat gtccgacgta taacgggttc caccagtggg ataccaataa
     1741 tcgaagaata gggcgcatgg ggcattgcca gaccaggaag cgccgaaatc cccagtcctg
     1801 cctccactag tcctaatgac gtcgaaagat gacgcacttc gtaaaaccag tccagcttcc
     1861 agggcttgtc ggctagctgt tgttctatca acaatcggtt gccgctggag gaacgtacgc
     1921 caatcatttt gtaacccact aactcctgcc attctacaag ctactttttg gccaatggat
     1981 ggtcacgccg acaggccagc acgaatggct cgttgaccag tggggtaaaa tcaatggatg
     2041 aatttgtgac gttgttcatg tttatgccaa agtctgattc gttacacagc accgattcca
     2101 tgcaattatt cgtgccttgt tccagaatcc gcactttaat attgggatac agctcattaa
     2161 atttaccgat tgccagcggt aaaaaataaa atactgcggt cggaatacac gctaatgtca
     2221 ccataccacg atgataagca ttcatatcac gaatattaaa aagcgtttca tcgaatttct
     2281 ttattaattc ccgcgcctcc gggagcaacc tttttcctgc ttttgttaac gtaaccttgc
     2341 gagttgtacg ctcaaatagt tgaatatgta aatcttcctc catctttttt attcttcgcg
     2401 ttaatgcagg ttgcgtaata ttgagtaatt tagcggcatt gttaaatgaa ctggactctg
     2461 ccagtatgac aaatgccttc atacttgata attcatgctt cattattact ccggaaaatg
     2521 gaagcgacga ttttgggggg ctggtcgtta aaaattttaa ctgcatttag ccaacttaaa
     2581 ttaatgaaaa aatgttatta atcgttgagc taaagtcatt agagatgctt tgtccttaat
     2641 gtaaccatat cgcaataagt tatgttttta aattgagggc attattatga aaaaaatacc
     2701 ctgcgtgatg atgcgaggtg gaacctcgag gggcgcgttc ctgttagcgg aacatttacc
     2761 cgaagatcaa acgcagcgcg ataaaatatt gatggcaatt atgggttccg gtaacgatct
     2821 ggaaattgac ggtattggcg gcggtaatcc actgaccagt aaagtcgcca ttattagccg
     2881 ttccagcgat ccgcgtgctg atgtcgatta tctgtttgct caagttatcg tccatgagca
     2941 acgtgtcgat accacgccta actgcggcaa tatgctgtct ggcgtagggg cattcgccat
     3001 tgaaaatggt ttgattgcag cgacttcgcc agttacccgt gtacgtatcc gcaacgtcaa
     3061 tacgggtacg ttcatcgaag ctgatgtgca aacgccaaat ggtgttgtcg agtacgaggg
     3121 tagcgccaga attgacggcg taccgggtac tgccgcaccg gttgcgctca ctttcctgaa
     3181 tgccgctgga acgaaaactg gaaaagtttt cccgactgat aatcagattg attattttga
     3241 cgatgtcccg gtgacctgta tcgatatggc gatgccagtc gtcattattc cggctgaata
     3301 tctgggaaaa acaggttatg aattaccggc ggaactggat gccgacaaag cattattagc
     3361 ccgcattgaa tctatccggc tacaagcggg taaagcaatg ggcttaggtg atgtcagtaa
     3421 tatggttatc cctaaacctg tgcttatttc tccagcgcag aaaggcgggg caattaatgt
     3481 gcgttatttt atgccgcatt cttgccatcg cgcgctggcg ataaccggtg ctattgctat
     3541 ttccagtagt tgtgcattgg aaggcaccgt cacccgacaa atcgtccctt ctgtaggata
     3601 tggcaatatc aatattgaac accccagtgg tgcgctcgac gttcatttaa gtaatgaagg
     3661 tcaggatgcc acgacgttac gcgcatctgt tattcggaca accagaaaaa tattttccgg
     3721 tgaagtttat cttccctgaa aaaattcgtt gtcaggataa ggacaatcaa taaaggactt
     3781 ctgtatgagt catacagaaa gaacaggatt ttaaatgaat aagaaatcgt tatggaagct
     3841 aattctgata ttagcgatcc catgtattat tggttttatg ccagctccgg caggattaag
     3901 cgaactggcg tgggtgcttt ttggtattta cctggcggcc attgtgggtc tggttatcaa
     3961 gcctttcccg gaacctgtcg tactgttaat tgccgttgct gcctcaatgg tggtggtcgg
     4021 taacttatcc gacggtgcgt ttaagaccac cgccgtatta agcggttact cttcaggtac
     4081 tacctggctg gtgttctcgg cgtttacctt aagcgccgca tttgtgacca ccggtttagg
     4141 taaacttatt gcctatctgc tgattggtaa aatcggtaac accatgctgg gtctgggtta
     4201 cgttacggta ttcctcgatc tggtactggc tccggcaaca ccgtctaaca ccgcgcgtgc
     4261 gggcggcatt gtgttaccga tcatcaacag cgtggcggtg gctttggggt ccgaaccgga
     4321 aaaaagtccg cgtcgtgtcg gacattacct gatgatgtcc atttacatgg tcaccaaaac
     4381 caccagctat atgttcttta ccgcaatggc ggggaacatt ctggcgctga aaatgatcaa
     4441 cgacattctg cacctgcaaa ttagctgggg tggatgggcg ctggccgccg gattgccggg
     4501 catcattatg ctgctggtca ccccgctggt aatttacacc atgtatccac cagaaattaa
     4561 aaaggtggat aacaaaacca tcgctaaagc gggccttgcc gaactgggac cgatgaaaat
     4621 ccgcgaaaaa atgctgctcg gtgtctttgt gctggcgcta ctgggctgga ttttcagtaa
     4681 gtctctgggg gttgatgaat ccactgtggc aatcgttgtt atggcgacca tgctgctgct
     4741 gggtatcgtt acctgggaag acgtggttaa aaataaaggc ggctggaata ccttaatctg
     4801 gtacggcggt attatcggct taagctcctt attatcgaaa gttaaatttt tcgaatggtt
     4861 agctgaagtc tttaaaaata acctggcatt tgatggtcac ggtaacgttg ctttcttcgt
     4921 tattattttc ctcagcatca tcgtgcgtta tttcttcgct tccggtagtg cctatatcgt
     4981 tgctatgtta cctgtatttg ccatgctggc gaacgtctcc ggcgcaccgt taatgttaac
     5041 cgcgctggca ctgttgttct ccaactccta tggcggcatg gttactcact atggcggcgc
     5101 ggcaggtccg gtcatctttg gcgtgggtta caacgatatt aaatcctggt ggttggtcgg
     5161 tgcggtactg acgatattaa ccttcttggt gcatatcacc ctcggcgtgt ggtggtggaa
     5221 tatgctgatc ggctggaaca tgctgtaaat atactcgtca tacttcaagt tgcatgtgct
     5281 gcgtctgcgt tcgctcaccc cagtcactta cttatgtaag ctcctgggga ttcactcact
     5341 tgtcgccttc ctgcaactcg aattatttag agtatatcca tttattatct ttctgcgcac
     5401 ttcacggtgc gcagatatct ggagcatttg atgatcaagt tatctgaaaa aggcgtgttt
     5461 ctcgccagta ataacgaaat aattgccgaa gaacatttca ccggcgaaat taaaaaagaa
     5521 gaagcaaaaa aaggcactat tgcctggtct attctctctt ctcataatac gtccggaaat
     5581 atggataaac ttaaaattaa gtttgattca ttagcctctc acgatattac ctttgttggt
     5641 attgtacaga ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc
     5701 aactgccata actcactctg cgccgtcggc ggcaccatta acggtgatga ccatgttttt
     5761 ggtttatcgg cagctcagcg ttatggcggt atttttgtgc ctccgcatat tgcggtcatc
     5821 catcaatata tgcgtgagat gatggctggc gggggcaaaa tgatcctcgg gtcagacagc
     5881 cacacccgtt acggtgcatt agggacaatg gcagtcggtg agggtggcgg tgagttggta
     5941 aaacagctgc ttaatgacac ctgggatatc gactatccgg gcgtggttgc ggtgcatctg
     6001 accggaaaac ctgcgccgta tgtggggcca caggatgtgg cgctggctat cattggcgcg
     6061 gtgttcaaaa acggttacgt caaaaacaaa gtcatggagt tcgttggacc gggcgttagc
     6121 gcgctctcta ccgatttccg taacagcgtt gacgtgatga ccacggaaac cacctgttta
     6181 agctccgtct ggcaaactga tgaagaagtg cataactggc tggcgctgca cggtcgcggt
     6241 caggattact gccagcttaa ccctcaaccg atggcgtact acgatggctg catcagcgtt
     6301 gatttaagcg ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa
     6361 atcgacacac tgaaccagaa cctgaccgac attctgcgtg agattgaaat tgagtccgaa
     6421 cgcgtggcgc acggtaaagc caaactctcg ctgctggata aagtggaaaa tggtcgcctg
     6481 aaagtgcagc aggggattat cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg
     6541 gcggcgaatg cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct ggcggtttac
     6601 ccgtcatcac agccggtgtt tatggatctc gccaaaaaag gtgtggtagc agatttgatt
     6661 ggcgcaggcg caatcatcag aaccgcattc tgtggcccat gctttggcgc gggcgatacg
     6721 ccaatcaaca acggtttgag tattcgccac accacgcgta acttcccgaa ccgcgaaggc
     6781 tctaagccag ccaatgggca gatgtcagcg gtggcgttga tggacgctcg ttctatcgct
     6841 gcgactgcgg caaacggtgg ctatttaacc tctgccagcg aacttgattg ctgggacaac
     6901 gtgccggagt acgccttcga tgtaacgccg tataaaaacc gtgtttatca gggctttgtg
     6961 aaaggggcaa ctcagcaacc gctgatttac ggaccgaaca ttaaagactg gccggaattg
     7021 ggtgcgctga ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga cgaagtcacc
     7081 accaccgacg aactgattcc ttccggtgaa acctcttctt atcgttcaaa tccgattggt
     7141 ctggcgggga atgtcagcga gctgacagag gtgtttgcgc gcattaagca gattgctggt
     7201 caggagcata ttgatccgct gcaaactgaa attggcagca tggtctatgc ggtgaaacca
     7261 ggcgatggtt ctgcgcgtga acaggcggcg agctgccagc gtgtgattgg cggtctggcg
     7321 aatattgccg aggagtacgc gactaaacgc taccgttcta acgtcatcaa ctgggggatg
     7381 ttaccgctgc agatggcgga agtgccaacc tttgaagtgg gggattacat ttacatccct
     7441 ggcattaaag cggcgctgga taatccgggt acgacgttta aaggttatgt gatccatgaa
     7501 gatgcgccgg taacggaaat tacgctttac atggaaagcc tgacggctga agagcgcgag
     7561 attatcaagg cgggtagttt gattaacttc aataaaaacc gtcagatgta aaaagcgcca
     7621 tgtgaatgta ggtcgcattc ggcacttatt gtcggatgcg atgctggcgc atcttatccg
     7681 acctacaaat cgcatcgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcaaata
     7741 gttaatagct cttacttctt ctcctctgca accactttgc tgcccacgcc gcggttattg
     7801 tattcccaca tgcggttgta gttagtgtca ttcagattgc gctgtatttc gtcgttatca
     7861 tctacgctgc cggtattacc cgcaaacgga cgattagaga tcaccgcatc ggcccacggt
     7921 ttagccgtgt taaaaccttc gttgatggcg ctatcacgga tcaccacctg accgttggta
     7981 ttagcatcaa catccagcga gcggcccagt tgcgccacgc catcaccgga agcattgaaa
     8041 cggctgttta cggcgaggaa accgtaatag atgttagaca gcgtagccgg tgcaaacaca
     8101 tatgcttctt gctgggtacg tgagttcacc acgcggaatt cggtgttatc gaacaccact
     8161 gcgccgcgac cagaaacgat atccacatcc ccttcaatat agctgttggt caccagcgta
     8221 cgcggctgac ggttggtttc cagacggttc tgcacaccgc tgttggtgac aaagaaggtg
     8281 ttctgacgac cgagaatgtt aacgttgtta atctgtactt ggtcaccgtc agtacgcagt
     8341 gctaccgccg gatggttacc tgcatctacg ctatcgccca gcgtgttttc gatggtcaga
     8401 ttttgcagtt gcagtccatt gttttgtgac cagaagaccg cagaacacag aacaccgata
     8461 ctgtcgctgc gtttgctctg gcagctatcg tacatatacc acgctggttt acctggcata
     8521 tatttgccgc gcgggttgac gtcgtgacgc cagtcggcag ggctcatgcc cccatcaagg
     8581 gaaagcccaa tcttcacatc aatcggtttt tcacctgtac cgtacagagt gattccacct
     8641 ggagcagcag ggacatacac cgttccctga tactcaccag gcatcacggc aatatactgg
     8701 cgcttgttgg tgcgcttgat aattgccgca tctaccgccg cctgaatcgt ggtatgcgtt
     8761 acaccttgag tacccgccgg gccgacaaca aagtcaggtt gcgcaggcag ggttatcggg
     8821 gacggattcc acgctgcagc acctggtgtc agggatgcaa aatagtgttg cgcatcgaaa
     8881 ttcagcgctt cttttgccga cagaatcggg cgagaagagg taccaggcgc agtttgatca
     8941 gaaggacgtt gatcgggtgg tgttgagcta caggcggtca gcgtcacgcc aaaagccaat
     9001 gccagcgcca gacgggaaac tgaaaatgtg ttcacaggtt gctccgggct atgaaataga
     9061 aaaatgaatc cgttgaagcc tgctttttta tactaagttg gcattataaa aaagcattgc
     9121 ttatcaattt gttgcaacga acaggtcatt atcagtcaaa ataaaatcat tatttgattt
     9181 caattttgtc ccactccctg cctctgtcat cacgatactg tgatgccatg gtgtccgact
     9241 tgtgcccgag aagatgttga gcaaacttat cgcttatctg cttctcatag agtcttgcag
     9301 acaaactgcg caactcgtga aaggtaggtg gatccccttc gaaggaaaga cctgatgctt
     9361 ttcgtgcgcg cataaaatac cttgatactg tgccggatga aagcggttca cgacgagtag
     9421 atgcaattat ggtttctccg ccaagaatct ctttgcattt atcaagtgtt tccttcattg
     9481 atatcccgag agcatcaaca tgcaatgttg tagggatggc aatttttacg cctgttttgc
     9541 tttgctcgac ataaagatat ccatctacga tatcagacca cttcatttcg cataaatcac
     9601 caactcgttg cccggtaaca acagccagtt ccattgcaag tctgagccaa catggtgatg
     9661 attctgctgc ttgataaatt ttcaggtatt cgtcagccgt aagtcttgat ctccttacct
     9721 ctgattttgc tgcgcgagtg gcagcgaccg ggtttgttgt tatatggcct tcagctattg
     9781 cctctcggaa tgcatcgctc agtgttgatc tgattaactt ggctgatgcc gccttgccct
     9841 cgtctatgta tccattgagc attgccgcaa tttcttttgt ggtgatgtct tcaagtggag
     9901 catcaggcag ccccctcctt attgctttaa ttttgctcat gtaatttatg agtgtcttct
     9961 gcttgattcc tctgctggcg aggatttttt cgtagcgatc aagccatgaa tgtaacgtaa
    10021 cagaattatc actgttgatt ctcgctgtca gaggcttgtg tttgtgtcct gaaaataact
    10081 caatgttggc ctgtatagct tcagt
//
LOCUS       DADLTY010000150        10096 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.151, whole genome shotgun sequence.
ACCESSION   DADLTY010000150 DADLTY010000000
VERSION     DADLTY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.151"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(52..531)
                     /gene="xdhC"
                     /locus_tag="JN334_003240"
     CDS             complement(52..531)
                     /gene="xdhC"
                     /locus_tag="JN334_003240"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HAY7439286.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="JN334_003241"
     CDS             complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="JN334_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HAY7439287.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="JN334_003242"
     CDS             complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="JN334_003242"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HAY7439288.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4129..4884
                     /locus_tag="JN334_003243"
     CDS             4129..4884
                     /locus_tag="JN334_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAY7439289.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4963..5036
                     /locus_tag="JN334_003244"
     tRNA            4963..5036
                     /locus_tag="JN334_003244"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4995..4997,aa:Gly,seq:ccc)
     gene            5171..6781
                     /locus_tag="JN334_003245"
     CDS             5171..6781
                     /locus_tag="JN334_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7439290.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6900..7232
                     /locus_tag="JN334_003246"
     CDS             6900..7232
                     /locus_tag="JN334_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439291.1"
                     /translation="MASEDWQREHHCRTGIIFTAFAIEAMFIFYRKQVDPGYDKTQKE
                     CRKTMHKNTLKLCGINNYMGTKPYQIIKECLEVRDAIAHGDSYTSSFNFSADHLDNQD
                     DIAKVSWR"
     gene            8351..9097
                     /locus_tag="JN334_003247"
     CDS             8351..9097
                     /locus_tag="JN334_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAY7439292.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
     gene            9395..9886
                     /locus_tag="JN334_003248"
     CDS             9395..9886
                     /locus_tag="JN334_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439293.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
BASE COUNT         2584 a         2515 c         2562 g         2435 t
ORIGIN      
        1 ttaaacacat aacctcagtg aatccggcct gaattcaggc cggatatcct tttacttcgt
       61 tttctcgcaa tccagaactg tatttacaat catctgatac cccgtgcagc gacaaagatt
      121 tcccgccagt ccgcgacgaa tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag
      181 cattgccgtg gtagccataa tcaggccagg cgtacaaaac ccgcactgca ctgtgccgga
      241 tttcgcataa gcctgctgaa catgagaaag ttttccgcct ttcgcttcac cttccagcgt
      301 gcggatctct tttccttcag cccaggcggc aaggtataag caactgtcta ttgctgtgcc
      361 gtcgaccaac accgtacagg caccacattc acccacgcag cacccttgtt tgacacttag
      421 cagtccttgt tcgcggagta attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa
      481 aggcatcccg ttaatggtgc attcggtggt aattgtttcg ctgtgattca ttgcaatttt
      541 ccccccgccg cggcgacggc ttcgctaatc acttttttgg tcatcgtctg gatgagatgc
      601 agacgaaact ctttactggc ccgccatgaa gaacgcgggg cgacatcttg caggacagat
      661 tcgctgatag cttccagcgt ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca
      721 tgttggcagc gaatcggcgt tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa
      781 ttgccgttat ccagtcggca atgtgcggcg cagccaatcg ttgaaatatc cattgcgtcg
      841 cgcatggcat atttaaaatg cgcgctgccc gcgtgttctt tcggctgtgg cggaaaatga
      901 aaggcgacga ggatttcgtc atgctcaaga gacactttgc ccggcccggt gtgaaagcca
      961 ttaatcggga cgaaacgaac accgcgcggg gagtggatct ccagtttcgc gtcataaatt
     1021 agcgttggcg tggcagaatc tgcgctggtg gcaccgttgc aaatatttcc accgtaggta
     1081 gcgacgttac ggatctgcgg tccagcaatg aacgtggccg cagcacataa cgccgggaga
     1141 tgacgttgag ttataggatc ttctattagc tgggtaaatg tcgttgcaga gccgatacgt
     1201 agcgagccat cttccgccag cgtaattccc cgcagctccg ccagattatg aatatcaaca
     1261 atatggcgat aacggtcatt gtgatggtgg agctgaatca gtacgtcagt gccaccggcg
     1321 agcagtttgg cctgcgggtt gtcagccagc aggttgatgg catcggcaag ggttgctgcg
     1381 cgatggtaag aagcaaaatc aaacatgatg ttatcctcaa atcaatcctg ccagatggaa
     1441 ctcttcatat aaccgttttg gcgtcagcgg cagtgtattg attgcaacac cggtagccat
     1501 cttcaccgcg ttacgaatag cagcggcaac aggaattatg ggtggctcac ccagtgactt
     1561 atgtccgtat gcggattgcg gctcattgat ttcgacgaac gcgctttcca gttgtggcag
     1621 atccggcatg gtcggcattt tgtaatccag cagattgggg ttacggacca cgccgctttt
     1681 agcatcgatg atcatctctt caaatagcgc ccagccaatg cccattccca ttccgccgtg
     1741 tacctgacct tctgccagca gtggattaag aatatgccct gaatcatgaa cgttgaggat
     1801 gcggttgatg gtgactttgc acagcgcaat atcgaccgtc agatcaacaa aggtacagcc
     1861 aaacgccggt gggttagtgg tggttttgat ggagctttca gcagagagct gcccgccgcg
     1921 ttcagggtgg tagaaagcgt ccatcgccaa atcttttaac gacattaacg gctcttccgg
     1981 tcgttcaacc agcacgatat ggccttttat cagggtcaaa ttcatcgctg actgatgtag
     2041 catgactgcg gcgtgagcga tgattttctc ttttaataat agtgccgcac tgcgcagcgc
     2101 aggcgcggca acatagctct ggcgtgaggc aaatgcgccg ggatcgaacg gcgtaacgtc
     2161 ggtatcttgt gttgaaataa cgcgaacgtc gctgaccgga acccccacgg tttctgccac
     2221 catttgcgag aagacggtgt cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt
     2281 gatggttcca tcctgattca tcagaaggcg cgcgcctgct atttctacgc cgacaggcca
     2341 ggtgttagag gtgtagctaa aacaggcgac gccaacgccg cggcgcaaat tgccttgctg
     2401 gttctggcat tctgcacggc gtttttccca ttcaaagatt ttccggcctt tttcaagaca
     2461 ctccggcaac cctgcgctgc aaatacgttt gcccgtgagc ggattagcat ctccttcgcg
     2521 ggcggcgttg cgtaaacgaa tttcaacagg atcaatacct aacgctgtcg cggcgtcatc
     2581 aagcatagac tcaacggcaa atacgacttg tggcgcgcca taaccacgca tcgcaccagc
     2641 cgaggggagg ttggtatagc aggtctttga actgtaagcg taggcacaac gaggataaag
     2701 gtaagcgact ttattccccc ccgcagaagc gatggagtgc ccgtgagatg cataagcgcc
     2761 ggtgttagac agaacatcca gactataacc tttcaaggtt ccgtcgcggt tcacgcccat
     2821 ttgcccgtca atagtaaaag cgtggcgggt acgggttgcg aggaaacact cttcacggct
     2881 aagggaaact ttcaccggaa tgccgccaag cttgctggtc aggaatgccg ccattggctc
     2941 ttccagtaca tcctgtttat taccaaaacc gccaccgaca aacggtttga tgactcgtac
     3001 gcatgaccag ggaatatcca gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg
     3061 ggtgctggaa acgatggtaa ttcgcgagtc atcctccatc cacgccagcg atgttacgct
     3121 ttccatgtga caatgttgaa taacgggagt ctgatagtgc ccctgtacct ggtagtcggc
     3181 ggcatcgatt gtttgttgga cattgcccgt cgacatcgtg ctttgtttca gtaaattgcc
     3241 gccgttatgg attggtgcag cgtcttctgt cagcgccgct tctggcgtgg tgataacggg
     3301 taattcttgc cactcaatgc tgaccaattg cgccgctttt tctgccgtga gttcatcgcg
     3361 ggccacgacg atggcaacgg cgtcaccatg atgacgaaca tggcgagtta gcagtgcgcg
     3421 atcggcggta tcgcgcttgt tttcgtcaag tgtccaggca tgcccagctg tagcgaatgg
     3481 aatatcaggc acatcttccc aagtaaaaat cgccagtacg cccggtaaac ttctggcttg
     3541 ttcatcatta atacttacgg cataaccatg tgcgataggg ctacgtacat atttcgcgta
     3601 acacatgccc gccataacat aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat
     3661 ggcatcgacg cgcatgcatg attcacccgt agcggttgct tcccgcgctt ccatagaaat
     3721 cccctcgatt tgaacatttg gttcttatgg caaagattta gtctaagcag gcaagattta
     3781 cgccagaaac gacgataagc gttaggggag gaaggaagtg tgatgacgtt aaaaaaacac
     3841 agatatgata cgaaacagga ctctgaatag ggacagtgat acgtatcaat atgagtggcg
     3901 ataaaatcaa tgtgataaat caggattatt acgcatcagg atgggaaata gggtaaaaag
     3961 tcaggaactt cgatacctct acagcgaata ttttgctgat gatacttccc tttgccaaat
     4021 gaaattaacc tctttctcta ttaccacgtt tttccagaag caagagattg ggtatcaaga
     4081 ggctggctgc tatgatatgg cgtcttgttt tttaagcgag gaaagatttt gagtgcggga
     4141 cgcctgaata aaaaatctct gggtatcgtg atgtttttat cggttggact gctattggcg
     4201 ggctgttcgg gtagcaaatc atccgataca ggaacgtatt ccggctccgt ttacaccgtg
     4261 aaacgggggg atacgctata tcgtatttcg cgcaccacgg gaaccagcgt aaaagagctg
     4321 gcgcgactga acggcatttc ccccccttac accattgaag ttggtcagaa actaaaactg
     4381 ggtggggcga aaagtagcag tagtacacgt aaatcaaccg ccaaatcaac gactaaaacc
     4441 gcatcggtta caccgtcatc agcggtaccg aaatcttcct ggccgccagt agggcaacgt
     4501 tgttggttat ggccaacgac agggaaagtt atcatgccgt attcgacagc agatggcggc
     4561 aataaaggga ttgatatctc agctccacgg ggtacaccta tttacgccgc gggtgcagga
     4621 aaggtggtgt atgtgggcaa ccagctgcgt ggctacggta atctcatcat gattaaacac
     4681 agtgaagatt acattacggc ttacgcccat aatgacacga tgctggtaaa taatgggcaa
     4741 agcgtgaagg ctgggcaaaa aatcgccact atggggagca cggatgcggc atctgttcgc
     4801 ctgcatttcc agattcgtta ccgtgcaacg gcaattgatc cgctacgtta cttgccgccg
     4861 cagggcagca agccaaaatg ctgatggcga attaatcagc agtcagcagc gcggcacttg
     4921 ctaaggagag cgtaaggttt ataatgcctt acgcatctcg aagcgggcgt agttcaatgg
     4981 tagaacgaga gcttcccaag ctctatacga gggttcgatt cccttcgccc gctccatgac
     5041 acttcttaaa gtctactaac ttactgaatc ctaagggata cggcttaacg ccttccgata
     5101 gttttactca ctgtaaccaa cactgtgact aactttgttg aagtagtgag cagacctaag
     5161 gtctcagtac atgctcaagt caagaaccta cctttatcag cgtaatggtg tcttttacat
     5221 ccgtcttagg atgaagacca ctagtcgcct gaccgcttcg cttccttctc acaaccgtta
     5281 taaactagcg tcagtatctt tacggaccaa ggatagacgt accgctatgg ctcactcacg
     5341 gcacatcaag tcagcactta aagcaatcca tgcagacaac cctaacgcct cttatgagga
     5401 gctacgggag cacctgaaga ccattgtcga gtgggaactt agtgtcagcc gtgatgacct
     5461 gaacgacccg gagtcctacc agctttacgt tgaccagtac gatgacatta agtccaacct
     5521 tcgggaagct gtagcgaccg agcgtctgac tgtagaccag catcgctata ttaatgacgt
     5581 tatcggtgtg cttaaggcct gtcaggatag actcaatggc gatagctctg gcctattgtc
     5641 ttaccttgag cctgagactg gcagtctaag accgtccgtc tccctatctg tattggcgga
     5701 acctgaagtc cctgaaccta aagcactgac cctcgcgtct ctcattgagc agtacgagca
     5761 ggagaacgcc cagaactgga agccagcgac cctaagcgaa aaccgagcgt cacactctac
     5821 gctaatcgag atattcgact atctggatat tcaggatgtc ggcaaggcga cccgtgcaga
     5881 tatgctcaga gttcgtgaag tcctccagca gctaccgaag aaccgcaagc aacgctttaa
     5941 gtctatgccg ctgtctgact tgctgaaccg ggagtctaag actgactgtc tggacgtcgt
     6001 taccattaac aataaatatc ttatcaagat ggctgctgtg tttaagtggg cggtacgcaa
     6061 cgacttgata gctaagaacc tgaccgaagg tcttgagctg aaagtccctc agcgtaaagc
     6121 ctccgacgct cgtgatgcgt tcagtcctga gcaggtaggg caactactgg tcgcagctaa
     6181 agcgtactcg cagaagacct caggtaagcc ataccattat tacgtcaccg ctctggctgc
     6241 gattacaggt gcgagactta acgaggtggc ccaacttcag gttaaagatg tcaggaccac
     6301 tgaggctggg accgttttta tccatatcaa cgaagacgat agcagcctgc cgggtaagag
     6361 tatcaagaac gcccacagtg accgctgtgt cccgttggta gatggtgctt atggttttgt
     6421 attggctgac tttatgtcac tcgtggaaga ccgtagaaag actgagggtg ataatgctat
     6481 ggtgttcaat ggcctcaagc taatgaagaa cggctacggt gagcaggtta gcaagtggtt
     6541 taaccgaacg ttgctgccca aagtcctcgc tgaccgtagc ggcttagctt ttcactcgtt
     6601 tagacataca gttgcgaccc agttaaaaca acatggggtc gagttagcgt atgctcaggc
     6661 gattatgggt catagctcag ggtctataac ttacgaccgt tacgcgaaag aggtcgaggt
     6721 ggaaacgctg aaagaaaaat tagcggagtc attgtcagta aaaaaaatag acggaaagta
     6781 atcgcatagg gatagcggaa tgtgaggacg taatggtcaa agaattttta gttagcaaag
     6841 aaaataagcc ttcatatgaa tgtatgaata ctgctgaaac ttgtttgggg ctggctggaa
     6901 tggcttcgga agattggcaa cgtgagcatc attgccgcac aggtataata tttactgctt
     6961 ttgctattga ggcaatgttc atcttctata gaaaacaggt tgaccccggc tacgataaga
     7021 cgcagaaaga gtgcagaaag acaatgcaca agaatacgtt aaaattgtgc ggtataaaca
     7081 attatatggg gacaaaacct taccaaatta tcaaagagtg tcttgaggta agagacgcta
     7141 ttgctcacgg cgattcgtat acttcgagct ttaatttctc ggcggatcat ttggataacc
     7201 aagacgatat tgcaaaagtg tcctggcggt aaactctgaa caatttcggg gattaactgt
     7261 tgagcgcctt gcaaaataca ttgaaatggc aaagcatatt gaccatgaga tttctgataa
     7321 tggttacagg ccttctgaag gttaccttcc agcagccgag cgcagaagtc tgatgttagc
     7381 ttttggcgtc agtggtgtgt caatgtggcc cagtgcttga gcgataatac acctcactat
     7441 agaggaatgg acccttaact atcactatag gggagagacc ctaagtcccc accttaatag
     7501 cagtcacgac ccatacacac attcattaag tctcaggctt ccggtagcgg accgattgga
     7561 aaccatccgc agacgcctag tgttaaatca ccttggcctg agaccattcc ttttaacgta
     7621 agctatcttc ccttttgaca ggcggggagc agtagaccac atccgacttt aagttggctc
     7681 tgtgataatc gctgcgcgga taactgaaga accaagacac cattactgag gtctgccatc
     7741 catttgtgga caggcggaaa ctcaaagagg ttagatcagt ggtgtctgag gttggagctt
     7801 tgagtcttga ccagcaggct ggaggagacc atgagtttat cttgttatgg attgtgttat
     7861 cacaattcca ataagaagaa ctttaagtcc agcctccact cccgccaacc cgaagtaaaa
     7921 ttatggtcgc tatgatgtgg tctccatgct tcgggtctta ctggaagtac tgaacacttt
     7981 cgcgtcaagt tctccgaagt tcattcctgt ttgactaaac tatcactata gggaccatat
     8041 ctcgcctctg acctcctgag agacggcaag cgagaaaccg tggtccacgc tagcaagcta
     8101 gccaccagcc taaggcaaga cagcctctgg tcagcctgtg tgttctttgg tctcgtgact
     8161 taagtctatc accacacacg ctcggacatg ctgtcgaacc ttcatctcta tggtcatcgc
     8221 agaccactaa ccccaattac ctcaagtcat tctgtttgcg tcctgttgcc tgctggtctc
     8281 cctttacgtg ggggcttgag taggtcagtg gggtgcgact tcagggtgca aacaaaagga
     8341 gtacacgtaa atgtcccaac aagaaatcag catcattaac ctcgaccagc ttgtgtccat
     8401 gacctcagtt gaaattgctg agctgacagg aaaagaacat aaacacgttt tacgagatat
     8461 tcgtaatatg gtcgaagagc ttaacggggc caaaactgag cactgctcaa ctttgagcag
     8521 cgagcttaac gggtccaaat ttgggctcgt tggcgaggaa gtatacaagg acgcgaaagg
     8581 tgagagccga acaatgtatc gtctcgaccg taagcatact ttcattctgg tcgctggcta
     8641 ttccgttcac ctccgcgcca agtgttacga ccatattcag acgttagagc gtcgagtcct
     8701 tcaactggaa gaccagaaga agcgagcagc cattcagtcc gctaaccgcc gaggcgtgac
     8761 ttggggcgac tactgcaaaa cctatggtct gcccgctcag aagctaatga ccgcacttct
     8821 ccagcatcgt ggcctctttc gtaagaaccc aatcagtaac gagtggtctg taaatccgaa
     8881 gtattccgac tgctttcgta tcattaagcc gtcagaccag aagttctcag caggtgggta
     8941 taacttccgg ttcaacgcta aggggctgga ggtcttcggt aagcctgaga tggttgacaa
     9001 gatgcgaggc atccttattg cattcactgg caccgaccag cagaagcaag agcacctcct
     9061 gaagctggca caatccggta aggtggaggg aatctaagat gatgattgac ccgcgcacac
     9121 ctgaggggcg catgactctc cgttaccgtg gctaccggac tgaagtcctc ctcagagagc
     9181 ttggtctaga cccggaagat gagacccgcc agcaccaatc ccgagacgag ctaatagccc
     9241 aactggtcgc tatgaaactg ccgcttaacc gttagtagct cacctactca atacctaatt
     9301 taaacacctt ttgagatgac cgatgcgggg agacccgtag gccttactgt gcctgcgata
     9361 ccgatagtct caactctcaa gaggagaatt acctatgatg ttcaacaaca acaactggaa
     9421 gctgtcagtc accgacatca acctttatga aaacactgtg agtctggacg gtcagtccta
     9481 tccgttgtcg ctcgctatca agaccctcat tccgggctac ctgtcaggct taccgtccac
     9541 cagccgcgag gcgatggagt tactggaagc tctggctgaa gctggcgtga ccattggtaa
     9601 cttcttcagt aatgacctga tgaccgccta ccaacgccgc cagatgaaca aacgagctga
     9661 agctgagcgt atcgctaaag agctagcgtc tcagaaagag cgtacccgcg aaatgttcat
     9721 gaccgaagac gaatggcaaa aagagctaca gcgccgcgaa caggtcaaag ctgaacgccg
     9781 gacctatggt gaaaacctcc gcagcgctac tcattccgct ggtcgctcac gtgccgctat
     9841 cgtcgctgac cttgagtcag gcggtaactg gatggacagc ttgtaagcaa gcgtccctca
     9901 tctgagaaca cacggtccct gactgcctgt tagggacttc aagtatttga ctcaaggaga
     9961 ttaattataa tgaatatgac taaaaacgct ttccagtttg gcattgagcc agtacgcatc
    10021 accgataccg acaacattca ggtcaatgaa ggtctgccga ctaacgccga ccctcaggtc
    10081 tatgccctcc agttag
//
LOCUS       DADLTY010000151         9932 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.152, whole genome shotgun sequence.
ACCESSION   DADLTY010000151 DADLTY010000000
VERSION     DADLTY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.152"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            68..862
                     /locus_tag="JN334_003249"
     CDS             68..862
                     /locus_tag="JN334_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAY7439294.1"
                     /translation="MDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQLTRVISSN
                     SETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRTYLYDAEQ
                     PYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENRGDISNSG
                     AYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYAYAPNPLG
                     WIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            962..1165
                     /locus_tag="JN334_003250"
     CDS             962..1165
                     /locus_tag="JN334_003250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439295.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            1183..1503
                     /locus_tag="JN334_003251"
     CDS             1183..1503
                     /locus_tag="JN334_003251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439296.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(1569..1801)
                     /locus_tag="JN334_003252"
                     /pseudo
     CDS             complement(1569..1801)
                     /locus_tag="JN334_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
     gene            complement(1911..2681)
                     /gene="yafV"
                     /locus_tag="JN334_003253"
     CDS             complement(1911..2681)
                     /gene="yafV"
                     /locus_tag="JN334_003253"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HAY7439297.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            2835..3308
                     /gene="ivy"
                     /locus_tag="JN334_003254"
     CDS             2835..3308
                     /gene="ivy"
                     /locus_tag="JN334_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HAY7439298.1"
                     /translation="MGRISSGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSAIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(3351..5795)
                     /locus_tag="JN334_003255"
     CDS             complement(3351..5795)
                     /locus_tag="JN334_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY7439299.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            6035..6613
                     /gene="lpcA"
                     /locus_tag="JN334_003256"
     CDS             6035..6613
                     /gene="lpcA"
                     /locus_tag="JN334_003256"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HAY7439300.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            6819..7586
                     /locus_tag="JN334_003257"
     CDS             6819..7586
                     /locus_tag="JN334_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAY7439301.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(7557..8297)
                     /locus_tag="JN334_003258"
     CDS             complement(7557..8297)
                     /locus_tag="JN334_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HAY7439302.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            8589..9202
                     /locus_tag="JN334_003259"
                     /pseudo
     CDS             8589..9202
                     /locus_tag="JN334_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            9378..9875
                     /gene="rayT"
                     /locus_tag="JN334_003260"
     CDS             9378..9875
                     /gene="rayT"
                     /locus_tag="JN334_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HAY7439303.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
BASE COUNT         2479 a         2521 c         2541 g         2391 t
ORIGIN      
        1 tgaccatgac cattattatt acgacaaagc gggcaacctg ctgacggatg agtatcaggg
       61 agcggtgatg gataaccgcc tgccgggtta cgggcgtgac cgctaccgct ataacgaatg
      121 gggcgaactg acagagcgca gggaccagca gctggagtgg aacgcgcagg gccagttaac
      181 gcgggtcatc agcagcaaca gcgagacgcg gtatcagtac gatgcgctgg gcaggcgcat
      241 cagcaaggcg accagcaatc tccataccga ccggggagaa cgaagccgga caacgtttgt
      301 gtgggaaggg ttccggctgt tacaggagac aacgtggcag ggaaagcgga catacctgta
      361 cgatgcggaa cagccgtaca cgccggtggc tgccatcacg gggcgcggag agagccagaa
      421 aatctggtat taccacacgg acctgacggg cacggtacac gaggtaaccg cgccggacgg
      481 gacgctggtg tgggcgggct accaggcggg atttggtgaa aaccggggcg acatcagcaa
      541 cagcggggcg tactttgagc agccgcagcg gctgccgggg cagtactttg acgaagaaac
      601 agggctgcat tacaatctgt tcaggtatta tgcgccggag tgcgggaggt tcgtaagcca
      661 ggacccgata ggattaaacg gtgggttgaa cttatatgct tatgcgccta atccgttagg
      721 ttggatcgat ccgctaggac tgaattcttt aggaatagat tcaaataata tatttagagg
      781 agatggtaac tataaaggtg gcggaattgg acagccaaag agtggcacaa tatcagcaca
      841 ggacattatt gatcatctct aagaaaaagg tgatgggcgt ttaacatctt ttagtactaa
      901 aaaaacatct agttccaact ctcgtggtgc agattttttc tcaaaaaacc atgtcttcaa
      961 agtggcagtt tctgatctcg atgatttagt taaaaataat cagatcactg ttttaacaca
     1021 agaaaaagta aaagcaatat ttggtttaga atcaagcaag aaaattagaa aagaacttgg
     1081 taatattctt cagaatatgg ctaggaataa tgaggtgtta gttcatgggc aaattcttgg
     1141 aaataaagtt agccgatgta attagttagt ggagaggaag ttgtggacga aattagaata
     1201 agaattgctg gtaatacggc tgcgccttgt ttccatgtgc taaaaaataa gggatataaa
     1261 gtaacaatgg aaactcatgc attatctgac gattgtttag attgtgtata tgattggtat
     1321 gcggaaaaaa atggacaatt ttttagtgca acgagtccag aagagttact tggattagtt
     1381 gcaatgtggg aagttcgtgg taatgactgg agagctaatt tgaatgagat aaatacttat
     1441 caaaaagcca tagaagaatc cccgtttttt gataaaaacg gcaatgaaat agagtctgac
     1501 taaattttat tgatatagca acgataataa ccggaaattt cttccagatc tttccggctt
     1561 tttattattt aactttcgtt cagcacacta aacatcccgt ctcgcatccc cttaactggc
     1621 tgtttaacct gataaagttc cctgcgcagt tcctgtactt cgttgttatc ccaagcagga
     1681 tcaaacatcc ctccgagatc ttcacggtaa cgcccgtgcc gatctcaaat cccgcttttc
     1741 tcagccattt ggcgctgatt ttgatcaccg gcgtgtcgct gcggttgggc agataaccca
     1801 cggtacagcg acgctgtgtt ttggaaatgg tgacttctgg cgtaatattg tgtttaagtt
     1861 aggggctttc gtaatctagc ccagcccccg gccctaaaca caacccccac tcaccacaac
     1921 ctaaactcat ccgcatcccg ccatgccgga aacttttctc tatattcccg caatgccacc
     1981 atcgacagct ccgcatcaat acgcgttgcc ttatgcgcgt cggcggtagc gataatctcg
     2041 ccttgcggat taatcacccg gctgtcaccg cgataatggc agccgttgcc atcgctgccg
     2101 acgcgattgc atcccgccac atacgcctga ttctcaatcg cgcgggccgt cagcaatgcc
     2161 tgccagtgca gagagcgcgg agctggccag ttggcgacgt acagggcgag gtcataatcg
     2221 ttgagattgc gcgaccacac tggaaaacgt aagtcgtagc acaccagcgg caaaatacgc
     2281 cagccgcgcc attccacaat cactcgcgca ttgcccgctt tataatgcag atgctcatct
     2341 gccatgcgga acagatgacg cttatcataa aaatgtaccg tgccgcccgg ctcaaccagc
     2401 aaaaagcggt taaccgaacc agactccgtt tgtaatgcaa cactgcctgc aatcagcgca
     2461 ttgcactgct gcgccttagc tgtcatccag ttcactacgt catcttgtgc aagcgacgaa
     2521 gctgccgctt ccatggcaaa gccgctggta aacatctccg gtagaacgat cacatcgcgc
     2581 ccggtaatac cttccagttg acgatcaaaa tggcgcaggt tggcaggacc atccacccac
     2641 accaacggtt gctgcaaaag cgtaatcttc aaactaggca cggtgtacaa ctcctttatg
     2701 cgaagggttt tataacttta acaccttatc aggcagttgc cttagcgcag aataaattga
     2761 taacaaatgc tgatattgga aatatctgat ttgcaaatta tcgtgttatc gccaggcttt
     2821 aggaggttaa taacatgggc aggataagct cgggaggaat gatgtttaag gcaataacga
     2881 cagtcgccgc actggtcatc gccaccagtg caatggcgca ggatgattta accattagca
     2941 gccttgcaaa gggcgaaacc accaaagctg cgtttaatca gatggtgcaa gggcataagc
     3001 tacctgcatg ggtgatgaaa ggcggtactt atactcccgc acaaaccgta acgttgggag
     3061 atgagacgta tcaggtgatg agcgcgtgca aaccgcatga ctgtggctcg caacgtatcg
     3121 ctgtgatgtg gtccgagaaa tctaatcaga tgacggggct gttctcggct attgatgaga
     3181 aaacgtcgca agagaaactc acctggctga atgtgaacga tgcgctttcg attgatggta
     3241 aaacggtgct gttcgcggcg ttgaccggca gcctggaaaa ccatccggat ggctttaatt
     3301 ttaaataatt agcggataaa gaaacggagc ctttcggctc cgttattcaa ttacgcggct
     3361 tcaactttcc gcactttctc cggcaacttt accggcttcg tcgccagctc ttccggatca
     3421 aagtcatcaa cgttaatact gcgcagacgg ctttcttccg ctttcaccag aatagcggct
     3481 tcatctttat caatcagccc cttcgtcagc gcgttgtgcg ccagttcatc cagacgggta
     3541 aatggcagat ttttacccag ctctttacag atccgctgat gaatcgggtc ggcggcaatc
     3601 acatccacca acgcctcttc cagtaaacca accgggttat gctcgcttgg cgtcaggtac
     3661 tgaccgcgac caatacgtga acgagtggcg ttcggcactt gtaaaatctt cgccatttta
     3721 tgatccagct tgtcagacgg tgccagataa tgacgtccgg tcgggaagat caccacattc
     3781 agcagcccgg caaccacgcg gttcgggaag ttttgcagta aattgtccat tgcctgttct
     3841 gcctgataca gcgcatcctg tacgccccag tgcaccagcg gcagatcggc ttcattacgg
     3901 ccttcgtcgt cataacgctt cagaacggca gaggcgaggt aaagctggct taaaatatcc
     3961 cccagacgtg ccgagatacg ctcacgacgt ttcaggctgc cgcccagcag tgccatcgag
     4021 acatcagaaa gcagggcgag gttggcgctc aggcggttca ggtgctgata gtagcgttta
     4081 gtggcatcgc cggttggcgt gctgctggtt aaaccgcgcg tcaggcccag ccagaagctg
     4141 cgaactttgt tgctaccgac gtgaccgata tgtttgaaca acagtttatc gaacgcgttg
     4201 acgtcattgt tcttcgccgc ttccatctct tccagcacgt acggatggca acgaatcgct
     4261 ccttgtccga agatcatcat gctgcgggtc agaatgttag ccccttcaac ggtgatggca
     4321 atcggtgcgc cctggtaagc acgcgccagg aagttgcttt gcccgagcat aatgccttta
     4381 ccgccggtaa tatccatcgc atcaataatc gactgctgcc cgcggtgggt acagtgatac
     4441 ttaacgatag ccgacagcac ggcaggtttt tcgccgagca taatgccgta ggtaatcagc
     4501 gatgccgcag catccattac ataagcgttc ccggcaatac gcgccagcgg ctcttcaatc
     4561 ccttccatct taccgataga gattttgaac tgacggcgaa tgtgagaata cgcgcccgtt
     4621 gccagcgcta ccgatttcac gccgccggtt gagttggaag gcagggtgat gccgcggcct
     4681 accgagaggc actccaccag catccgccag ccttgcccgg ccattttcgg cccgccgatg
     4741 atgtaatcga tcggcacgaa gacatcttta ccgcgcgtcg gtccgttctg gaacggtacg
     4801 ttcagcggga agtggcgacg accaatttcc acgcccggcg tggtggttgg gatcagcgca
     4861 caggtaatgc ctaaatcttc tgcaccgccg agtaattttt ccgggtcgga gagtttaaac
     4921 gccagcccaa gcacggtcgc aatcggtgcc agcgtaatgt agcgtttgtt ccaggtcaga
     4981 cgcatcccca gcacctgctg gccctgccat tcgcccatgc agacaatccc ggtgtccgga
     5041 atcgcgcccg catcggaacc cgcttccggg ctggtcagtg caaagcaggg gatctcctga
     5101 ccacgcgcca gacgcggcag atagtgatct ttctgctcgt cagtgccgta atgttgcaac
     5161 agttcgcccg ggcctaatga gtttggcacg ccgacggtaa tcgccaggat cccgctcacg
     5221 ccagagagtt tttgcagcac acgagactgg gcataagctg agaactccag tccgccgtac
     5281 tcttttttga taatcatcgc gaagaagcga tgctctttaa ggtacgccca caactccggt
     5341 ggcagatccg ccagctcatg agtaatctgg aaatcattcg ccatccggca ggcttcttct
     5401 accgggccgt cgagaaacgc ttgctcttca gcggtcaggc gcggctgcgg atagttatgc
     5461 agctttttcc agtccggctt gccctggaac aagtcgccct cccaccaggt ggtgcccgca
     5521 tcaatcgctt ctttctcagt gcgcgacatc ggcggcatca ccttacggaa accgcgaaat
     5581 accggcgcgg aaatcatcga cttacgcata ggcgcaaagt taaatggcac gaggataatg
     5641 gtcagaggca ccagtaccca cgccgaccac agaccagcaa cgccgagggc ggctgtccag
     5701 gcgagcaaaa tcagactgct gataaataag ctcacgcggt gatagaacaa cgcgccgagc
     5761 aggacaaccg tagcgagaat actcaaaatc atcataacga aaagcccctt acttgtagga
     5821 ggtctgacca cttgtgatga tatggttgta gtggatgtaa aaacatttag caatgtgttt
     5881 acaatataat tacaacaaag ctcacattgt tgctgttttt atccgcactt caggtcaaaa
     5941 agtcctggtc atagcacctg cccgtacttc tcgcttttgg cggtatccgg tacactgcat
     6001 tttgtctatt acatttatgc tgaaggatat cctcatgtac caggatctta ttcgtaacga
     6061 actgaacgaa gcggcggaaa cgctggctaa ctttttaaaa gatgacgcca atattcacgc
     6121 cattcagcgc gcggcggtcc tgttagcaga cagctttaaa gccggtggca aagtgctttc
     6181 ctgcggcaac ggcggttccc attgcgacgc tatgcacttt gccgaagagt tgaccggtcg
     6241 ctaccgtgaa aaccgtccgg gctacccggc gattgctatt tctgacgtta gtcatatttc
     6301 ctgcgtcggt aatgatttcg gtttcaatga tattttctcc cgctacgttg aagcggtagg
     6361 tcgcgaaggc gatgtactgc tggggatctc cacctccggt aactctgcaa acgtgatcaa
     6421 agcgatcgca gcggcgcgtg agaaggggat gaaagtgatc accctgaccg gtaaagacgg
     6481 cggtaaaatg gctggcacgg cggatatcga aattcgcgtt ccgcactttg gttatgccga
     6541 ccgcattcag gagattcaca ttaaagtgat ccatatcctg atccagttaa ttgaaaaaga
     6601 gatggttaag taagtctggc gtaggccgga taaggcgttt acgccgcatc cggcacttgt
     6661 gcgctgatgc ctgatgcgat gctgacgcgt cttatcatgc ctacaaatct gtacccgaac
     6721 cgtaggccga ataattcgtt tacgccgcat ccgacctgaa aattcttaaa tcaatcttcg
     6781 ccggggtcca tgcgctcccg ctgttgtgga ggttacccat gtgcgaattg ctcgggatga
     6841 gcgccaacgt ccctaccgat atctgcttta gtttcaccgg acttgtacag cgtggtggtg
     6901 gaaccgggcc acataaagat ggctggggca ttacctttta cgaaggtaaa ggctgtcgca
     6961 catttaaaga tccacaaccc agctttaatt cccccatcgc caaacttgtc caggactacc
     7021 cgataaaatc ctgttcggtg gtggctcata ttcgccaggc taatcggggc gaggtggcgc
     7081 tggaaaatac tcacccattt acccgcgagt tatgggggcg taactggact tatgcccata
     7141 acggacaact gacgggctac aaatcactgg aaaccggcaa cttccgcccg gtcggtgaaa
     7201 ccgacagcga aaaagccttt tgctggctgc tgcataaatt aacgcagcgt tacccgcgca
     7261 cgccgggcaa catggcggca gtgtttaaat atatcgcctc actggcggat gaactgcggg
     7321 cgaagggcgt tttcaacatg ctgctttcgg acgggcgcta tgtaatggcg tattgctcga
     7381 ctaatttaca ctggatcacc cgccgcgcgc cgtttggcgt ggcaacgttg ctggatcagg
     7441 atgtggaaat cgacttcagc tcgcagacca caccgaatga tgtggtcacg gtgattgcga
     7501 cacagccgct gacgggcaat gaaacctggc aaaaaattat gccaggcgaa tggcgcttat
     7561 tttgcctcgg ggagcgtgta gtttgatgcc agttgtggct gcaccacttc gtggcttaac
     7621 ggcttgctga ccacgtaacg gccattggcc acagaaacgg tcggtggctt acgggtttgc
     7681 tcaaagtagt cgtagcccgg cttcagctgc tcccagaagt ccttaaagtt ggaatattta
     7741 tggcgcttca tattggcgtc ggtcatgcgg aatgggtaaa tactcacctg cacgctcggc
     7801 tgaccaaaca ccagcgcacc agtaacgaac tggaagatct catcaatacc ctgattggtc
     7861 attgcgtagc agccgatgga tacacaatcg ccgtggatca tcaggtattt cccttcataa
     7921 ccatgcgcac ggtcataagc attggggaat ccaatattaa tcgctttgta gtaacggctg
     7981 tctggtttta actgattacg ctggacgcta taaaaccctt ccgggctttt gaaatcgccc
     8041 tgacgctgtt ttggccctaa gccgccggaa tatttacaga ttttataact gtcgagcagt
     8101 tgatattgct cgcccatttt gacgtaaaga tcgagcgtac gttcttcctt gaagatctgg
     8161 atgtagacag gggatcccat caactgctgc ttatactctt tgctcactgg cgtggtcgag
     8221 ctactgctgc ccagcaaacc ggcaaacgaa acgcacggga tcaacaacat cgcaagaatt
     8281 aatgcgattt tacgcatact gcttattcct tgataaaacg gttacacacg ccaggacggc
     8341 aaaatggatc ccaaatcgga atagtctgga tttggaaggc tcacattatc accaaaagag
     8401 ttttacgcaa gcctgtcggc gctgggttta caaatttagc acaggggcag ttttcaatag
     8461 tccgaaaagc gcatgtatca tttatttcct aatattatga aatctcaata gtaatatgca
     8521 aataagcaat taataatccc gtttactcct ccggtctttt aaaattctca ccttcattca
     8581 gccggtttat gtctttcatg tcttcgttct tgctcggcag atttttgcat ccaggcgtgt
     8641 tttcgttatg cgttttgctc cctttactcg ccagcgccac cacaaccggg agcagataaa
     8701 acagcatcca gaatggtttc ctgcgccgct caaggcgagt gacagacgct ggcaggcgct
     8761 ggcggaaaac aaccactttt taagcagcga tcacctgcat aacatcaccg aagtggcgat
     8821 tcaccgcctg gagcagcagc ttggcaagcc ttacgtctgg ggcggcacgc ggcccgatca
     8881 aggcttcgat tgtagtgggt tagtttttta tgcctacaac aagatccttg aggcgaaact
     8941 cccgcgcacg gccaatgaga tgtaccacta ccgtcgggca acgattgtgg cgaataacga
     9001 cctgcgccgg ggagatttgc tatttttcca tatccacagc cgcgagattg ccgaccatat
     9061 gggggtgtat ctgggcgacg gtcagtttat tgagtcactg cgcaccgggg aaaccattcg
     9121 ggtaagccgg ttagccgaac ctttctggca ggaccatttt ttaggcgcgc gcaggatttt
     9181 gacggaagag atgattttgt aggccggata aggcgtttac gccgcatccg gcggttgtgc
     9241 acggatgcct gatgcgacgc tgacgcgtct tatcatgcct acaaatctgt gcgtttttgc
     9301 gagccgcttt cccgatataa cacagcgtca ttgtcggcct ttccttttcc tcttaccgtt
     9361 gagaaaaagg agtcgctatg tcggaatatc gtcgttatta catcaagggg ggaacatggt
     9421 ttttcacggt aaatttacgc aatcgtcgaa gccatcttct gaccacccag ttccagacgc
     9481 tccgtaacgc cattattaac gttaagcgag acagaccttt tgaaattaac gcctgggtcg
     9541 ttttgccgga gcatatgcat tgcatatgga cattacccga aagcgatgat gatttttcct
     9601 ctcgctggcg ggagattaaa aagcagttca cccatgcttg cggattgaaa aatatctggc
     9661 aaccgcgttt ttgggagcat gccattcgca atacaaaaga ttatcgccat catgttgatt
     9721 atatttatat aaatccagag aaacatggct gggtaaagca ggtgagtgat tggccattct
     9781 caacgttcca tcgcgatgtc gcgcgagggt tatatcccat cgattgggcg ggagacataa
     9841 cggatttaag tgccggggag cgtatcattt tataactgtg cgctggtgcc ggatgcgctt
     9901 acgcttatcc ggcctacaaa tcgtgcttta ac
//
LOCUS       DADLTY010000152         9930 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.153, whole genome shotgun sequence.
ACCESSION   DADLTY010000152 DADLTY010000000
VERSION     DADLTY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.153"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(2..298)
                     /locus_tag="JN334_003261"
     CDS             complement(2..298)
                     /locus_tag="JN334_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HAY7439304.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            513..1241
                     /gene="tonB"
                     /locus_tag="JN334_003262"
     CDS             513..1241
                     /gene="tonB"
                     /locus_tag="JN334_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HAY7439305.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="JN334_003263"
     CDS             complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="JN334_003263"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HAY7439306.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1784..2323)
                     /locus_tag="JN334_003264"
     CDS             complement(1784..2323)
                     /locus_tag="JN334_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAY7439307.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2353..3096)
                     /locus_tag="JN334_003265"
     CDS             complement(2353..3096)
                     /locus_tag="JN334_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HAY7439308.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3124..3213)
                     /locus_tag="JN334_003266"
                     /pseudo
     CDS             complement(3124..3213)
                     /locus_tag="JN334_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3453..4091
                     /gene="ompW"
                     /locus_tag="JN334_003267"
     CDS             3453..4091
                     /gene="ompW"
                     /locus_tag="JN334_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HAY7439309.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(4151..4657)
                     /locus_tag="JN334_003268"
     CDS             complement(4151..4657)
                     /locus_tag="JN334_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY7439310.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(4703..5203)
                     /locus_tag="JN334_003269"
     CDS             complement(4703..5203)
                     /locus_tag="JN334_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY7439311.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(5289..5468)
                     /locus_tag="JN334_003270"
     CDS             complement(5289..5468)
                     /locus_tag="JN334_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAY7439312.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(5848..6654)
                     /gene="trpA"
                     /locus_tag="JN334_003271"
     CDS             complement(5848..6654)
                     /gene="trpA"
                     /locus_tag="JN334_003271"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAY7439313.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(6654..7847)
                     /gene="trpB"
                     /locus_tag="JN334_003272"
     CDS             complement(6654..7847)
                     /gene="trpB"
                     /locus_tag="JN334_003272"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAY7439314.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(7859..9217)
                     /gene="trpCF"
                     /locus_tag="JN334_003273"
     CDS             complement(7859..9217)
                     /gene="trpCF"
                     /locus_tag="JN334_003273"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HAY7439315.1"
                     /translation="MQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLLI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(9221..>9930)
                     /gene="trpD"
                     /locus_tag="JN334_003274"
     CDS             complement(9221..>9930)
                     /gene="trpD"
                     /locus_tag="JN334_003274"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HAY7439316.1"
                     /translation="AAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNANKSRQAL
                     DELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGVYSPELV
                     LPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDFGLTPYH
                     QEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANAQTVLEV
                     LRSGSAYDRVTALAARG"
BASE COUNT         2532 a         2481 c         2415 g         2502 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tagtttttct actgaaatga ttatgacttc aataaccctt gatttacctc
      541 gccgcttccc ctggccgacg ttactttcgg tctgcattca tggtgctgtt gtggcggggc
      601 tgctctatac ctcggtacat caggttattg aactacctgc gcctgcgcaa ccaatttctg
      661 tcacgatggt tgcgcctgct gatctcgaac cgccacaagc cgttcagccg ccaccggagc
      721 cggtggtaga gccagaaccg gaacctgagc cgatccccga accgccaaaa gaagcaccgg
      781 tggtcattga aaagccgaag ccgaaaccta agccaaaacc gaagccggtg aaaaaggtac
      841 aggagcagcc aaaacgcgat gtcaaacccg tagagtcgcg tccggcatca ccgtttgaaa
      901 atacggcacc cgcgcgcccc acatcaagta cggcaacggc tgcaaccagc aagccggtta
      961 ccagtgtggc ttcaggacca cgcgcattaa gccgtaatca gccgcagtat ccggcacgag
     1021 cacaggcatt gcgcattgaa gggcaggtta aagttaaatt tgacgtcacg ccggatggtc
     1081 gcgtggataa cgtacaaatc ctctcagcca agcctgcgaa catgtttgag cgtgaggtga
     1141 aaaatgcgat gcgcagatgg cgttatgagc cgggtaagcc aggcagtggg attgtggtga
     1201 atatcctgtt taaaattaac ggcaccaccg aaattcagta agcagaaagt caaaagcctc
     1261 cgaccggagg cttttgacta ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca
     1321 accgctacat acttaaataa tgcttctgtc gctttatagc gttgcccgat tggttcagac
     1381 gctacttttt tcacccacac ttcaatatta atgctgaccg atgtcgtccc tttctggaca
     1441 cagcgtgcat agcagcacac cacatcgccg accgcaaccg gccgtaagaa agtcattcct
     1501 tcaacccgca cagtcactac gcgaccgtgg gcaatttctt ttgccagaat agcgccgcca
     1561 atatccattt gtgacattaa ccaaccacca aagatgtcac cattggcatt ggtatcggcg
     1621 ggcatggcta aagtacgtaa aacaagatcg ccctgaggga cgttatgtgt tgtagacatg
     1681 gtaaaaccga cttaaaagga aaatcacagg cgcgatgcta ctatgatttt cctgaggaga
     1741 acagaggaga tacagcgcca gccccgaagg actggcagtc tggttaggat ttatcttcct
     1801 gcggcatgtg gcggtagata tagataccgc ttaacaatgt gaagattaag gtaagggcgg
     1861 tcaggccaaa gactttaaag ttgacccaaa tattttgcgg cagccagaat gcgatgtaga
     1921 tgtttgccag accgcaaagg ataaagaaaa cagcccaggc cagatttagc ttcgaccata
     1981 ccggttgcgg cagcgtgagt tctttactca gcatccgctg aattagcggc tttttcatca
     2041 cccattggct gactaacagg gctcccgcaa acagagcata aatgactgta accttccatt
     2101 taataaactc atcattgtgg aagaacagcg tcaagccacc gaagacgacc accagaacaa
     2161 aagtgatcag ggccatcttc tcaaccttac gaaagcgaac ccagctatag ataagcacaa
     2221 tcgccgtggc gacgatcagc gctgcagtag ccgcatagat gtcataaatc ttgtaaaacg
     2281 cgaaaaagac aaccagcggt aaaaaatcaa gaaactgctt cattttacga ttccgtaatc
     2341 aaatgcttaa ggttattggc gaatcaacat atacaggcgg aacagataga tgagcaatat
     2401 ggcagaaatc aggttgctca aggtgttcgc cagtacggca ccaatttccg gggttaatgc
     2461 ggcaaaagaa gaggcaaaaa gcagcaacag tgtttttgcc agcaaccagc tcagtactgc
     2521 gggtgccacc agacgcatat tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa
     2581 aatgcccatt ttgtcctgta ccagcatcac cggtgccagg gccagtaaaa tggccatgat
     2641 aattcccgga acgaccacca gcatgatgcc aatctgtact aaaagggtag tcagaaaaat
     2701 cagaataaat aacttgggca atatcggcgc actggcacca atagctcgca gcgcactgac
     2761 tctctgaccc gcagacacca gctggataat taatattacg cctccggcga gaatggcgtt
     2821 accgattaat tctgaaaaag tggacgccgc tgaagcctgc agcaaaattt gttgctgttc
     2881 cggtgacata ttctgaacca ggtcgaacaa cccactactg ccgctaacgg gcacgccgtc
     2941 attgagctgc gcaagctgtg catcactggg tgagaaaaca tgccctaaca ccactgtgat
     3001 aaacgcacat agcaacgata ccaacagaat ggtcataaat tgattacgga agaaatttcc
     3061 ggtgtcacgg tatacggact gcgccgtgat agacatgcac tctccttgag ttttatgcag
     3121 gtgttaatta gcgggcaatt gtaccctggt taagcctcgg gtggcagcat caggtcttgt
     3181 atggaaaagc atatctttgt aaagcggagg taattgctgg ccttcaggcg tgggcaaagc
     3241 gcggcaagtg gcgatcgagt taatatttgc gtagagaaaa tatttaaaaa ttgatttaaa
     3301 tcacattaac caggattctc aatgcaactt ctaaattaat ccagatcaat aaatggtgaa
     3361 ttatcatatg taatgtgatc tatgtaggat catttgttac tccaatgtag gtatattcgt
     3421 cacgttttta taaccataac gacggagcgg atatgaaaaa gttaacagtg gcggctttgg
     3481 cagtaacaac tcttctttct ggcagtgcct ttgcgcatga agcaggcgaa ttttttatgc
     3541 gtgcaggttc tgcaaccgta cgtccaacag aaggtgctgg tggtacgtta ggaagtctgg
     3601 gtggattcag cgtgaccaat aacactcaac tgggcttgac gtttacttat atggcgaccg
     3661 acaacattgg tgtggaatta ctggcagcga cgccgttccg ccataaaatc ggcacccggg
     3721 cgaccggcga tattgcaacc gttcaccatc tgccgccaac actgatggcg cagtggtatt
     3781 ttggtgatgc cagcagcaaa ttccgtcctt acgttggggc cggtattaac tacaccacct
     3841 tctttgataa tggatttaac gatcatggca aagaggcggg gctttccgat ctcagtctga
     3901 aagattcctg gggagctgcc gggcaggtgg gggttgatta tctgattaac cgtgactggt
     3961 tggttaacat gtcagtgtgg tacatggata tcgataccac cgccaattat aagctaggcg
     4021 gtgcacagca acacgatagc gtacgcctcg atccgtgggt gtttatgttc tcagcaggat
     4081 atcgttttta attccgcaca aaaacgaccc cgtaatatac ggggtcaata aggacatggt
     4141 ataaagggta ttatttcttc gcttctacgc catcagtttc agaacgaatt aaaaatttct
     4201 cagttgtttg cggaatatgc tgaatcagcc agtcggccat ttgcttttcc tcatttaaaa
     4261 tcgcttcgat gatcggaatt gagactgtat caccggcatt ttttgctgct gctaatagtg
     4321 aggtgtaaca ggtgatttca aattgctcga agacatatcc gccaatagag cctttaacta
     4381 tttcatcaga agggaatata ccaccgattg actgcccaag cgcagccatt ttactcatgg
     4441 aatctttaat gactgaacgt gaaatgtcat tacgatcaag aatagtttcc agttgaacaa
     4501 tctggttttt ggtttcacta agatgttgtt caatacgagc gcgtagttca ggataattat
     4561 ctatacggct ggccatggat tcaagcatag attcggcttg cttttccatt gcgtgggcgt
     4621 cacgtaacca gtcatgataa tgttcaatac gattcatgaa aatgtcctct ttataaaatg
     4681 actaaaagtt aaaattcata tttcaggctt tattttcggc tttcttattt acgttattaa
     4741 gggccagatc agtcagtttg atgtcggtgg ccttttcttc ttccagggtt tctttcagaa
     4801 gcttcgctgc tttacggtaa cctaattgtt cagccagcgt cgctaatgtc ccgtaactgg
     4861 caatctcata atgctcgact ttctgtgctg cggcaatcag tgcggcatca cgcacttcgt
     4921 ttttctcggt actttcgatg acctcattag cttcttcaat aagaccttcc attgccacac
     4981 atttcatgcg cttaattttc agatttgatt ctgattccac aacttgatca attcgttcaa
     5041 tctgtccatg agtttcctcg aggtgcgcat gaaaagcctg acttaatttt tcatttgatg
     5101 ttgctcttgc gagttttgcc agtgcccggg ttaattgttt ttctgcgctg taggtatctg
     5161 aaagcagttg aataaataca tcttcaatgg tcttcatatt catatttttc tccagtgaaa
     5221 tcactgcgag ccatacggcc cgcagatgca attatcttta tcagctgata ttaataatta
     5281 acggtgaatc aggatttgcc tgatttatta ccaccgcttt gttgaccgcc ttttttaccc
     5341 gcttcagatg cgcgttgcgg atcattttta aaattaccgc cgctatgctg accgccttta
     5401 cggcctgcgt cggatgcctt ctcacggtct tcggcgaaat ttcctgaact accacgatgt
     5461 tcggccatgt tatttctccc gttgcgttgc attgtttcat taatatgagt gttgtgtgtc
     5521 gacactcatt aaaattagtc actaatgaga attagtcaaa ttaagcgcaa cgagaagata
     5581 gagggaaata tattttgagg aacattctgg atatattaac aattacctga ggaatatgtg
     5641 acttagagaa aattattgat tttactggtg ttatgttgcg ggattaattt gtttgattat
     5701 attactgttg ggcggaaaaa tgacgtaagt tgacgttcga ccggggtaag cgaaacggta
     5761 aaaagataaa tattaaatga atttaggatt tttccggctt cattaaagaa agttaaaatg
     5821 ccgccagcgg aactggcggc tgtgggatta actgcgcgtc gccgctttca tcggttgtac
     5881 aaaaactttc agtgccgcca gcattttctc tggctcatta atatgttgct cgatgatttt
     5941 aacaatggcc gaaccagaaa tcgcgcccgc agctcctgca tcaatcgctg cttttacctg
     6001 atccggggcg gaaataccaa acccctgcaa tggaggtgca gcgttgtact ctttcagctt
     6061 cgcaaccaga tgattgaggg gtaacgcggc gcggttttct gcgccggtca cgcccgctcg
     6121 tgacagcaaa tatgtgtaac cacgaccgta agaggctatc tggcgcagca ggtcgtcatc
     6181 ggcatttggc gggcagatga agataggtgc gacattatgg cgcaacgcgg cctggcggaa
     6241 gggcgcggac tcttcaactg gcacatcggc aaccagcacc gaatcgacgc cgactttctc
     6301 gcactgggca taaaactcat caatgccttt gttaaacacc agattggcat acatcaacag
     6361 gccaatggga atggtcgggt gtttctggcg aatcagtgcc agcatttcaa aacattgtgc
     6421 cggagtcaca cctgccgcaa aggcgcgcag agtggcgttt tgaatggtcg ggccatccgc
     6481 cagtgggtcg gagaagggga tacctaactc cagcgcgtca gcaccggctt caattagcgt
     6541 atcgataatt ttcaatgact gctcaatgcc cggatcaccg agcgtgacga aaggaacgaa
     6601 tgcgccttct ttgcgctcct tcaactgggc aaacagagat tcgtagcgtt ccatcagatt
     6661 tcccctcgtg ctttcaaaat atcgtgaacg gtgaagatgt ctttatcgcc gcgaccggaa
     6721 aggttaacca ccagtagctg ctctttatcc gggttttcgc gcatcatttt cagcgcatgg
     6781 gccagggcgt gggaggattc cagcgccggg atgatccctt cgtgcaggca cagcgttttg
     6841 aaggcttcaa gggcttcatc atcggtaata gacacgtaat cagcgcgtcc agtgctgtta
     6901 agatacgcgt gttgtggacc gacagacggg aaatccagtc cggcggagat ggagtaagat
     6961 tcttcaatct gcccgtcttc ggtttgcatc atcggcgctt tcataccgaa ataaatgccc
     7021 acgcgaccat gttttagcgg tgcgccgtgc tcgccagttt cgataccgtg accaccaggc
     7081 tccacaccaa tcaggccgac gttggtttca ttgatgaaat cagcaaacat gccgatggca
     7141 ttcgaaccgc cgccaacaca ggcgataacg gcatccggca ggcgaccttc tctttccaga
     7201 atctgcgctt tggtttcttc gccaatcatc cgctgaaact cacggacaat ggtcggataa
     7261 ggatgcgggc cagccgcggt gcccagcata tagtgcgcgg tttcataact gccggaccag
     7321 tcgcgtagcg cctcattaca ggcatctttc agcgtggcgg aaccgctatg taccgggatc
     7381 acttccgcac ccattaagcg catacgaaaa acgttaggcg actggcgttc aacgtctttg
     7441 gcacccatat aaatacggca tttcaggccg agcagggcgc tggcaagggc cgacgccacg
     7501 ccatgctgac cggccccggt ttcggcgatg atttcggttt tacccatccg cttcgccagc
     7561 aacgcctgcc ccagcacctg gttggtttta tgtgcgccgc cgtgcagcaa atcttcacgc
     7621 ttgagataca gcgtggtgtt cgtcccggct gtaatgttct ggcatttggt cagtgcggtt
     7681 ggacgcccgg catagttttt cagcaggtcg ttgaactggg cttgaaattc aggatctttt
     7741 tgcgcgctga caaaagcttc ttccagctgg cgcagagcag gcatcaggat ttgtggcacg
     7801 tacatgccgc caaactcacc aaaatagggg ttaagtaatg ttgtcattgt tcctttcctt
     7861 aatatgcgcg cagcgtctgg aaaaccgagg ccaaaagacg tgcgtctttg atgcccggtt
     7921 gcgactctac agcagaatta aaatcaagtc cggcgcagcc ggtttgtgcc gcttccacgc
     7981 agttatctgc gcctaagccc cccgccagca gaacgttgcc aagcgattga ccatttaata
     8041 gtgaccagtc aaaacgttgc ccgcttccac cctggccgtt gtctaaaaca tatttatcaa
     8101 cgtgctgaaa ctcgcgggcg ggcagggttt caccgacgct taatgctttc cagatggcaa
     8161 catgtgctgg cagagcttca cgcagcgtat cgatatacag ctgatcttca ttgccatgca
     8221 gttgcactgc cgccagcgat aacaccttag ctttgtccac cacatcggca atatcgtgat
     8281 tgcggaacac gccaacatac tgcaacggtg ccgcagccat cacttcctgc gcctgttcaa
     8341 cgttgacgca acgcggtgat gtcgcaacaa aaatcaaccc accgtaaatc gcgcccgcgt
     8401 cataagctgc tttagcatct tgcccacgcg tcaggccaca tactttattc tcacccagca
     8461 acacccggcg cacggcggcg tgcaaatcgt catgggccat caacgccgaa ccaatcagaa
     8521 aaccgttagc gaagtggctt aactcgcgca cctgagcgta agtattgatg ccggattcgc
     8581 tgattaccgt tacgttgtgc cccagtttcg gcgcaagctc gcgggtacgg ttgagatcaa
     8641 tcgacaaatc acgcagatcg cggttgttga tgccaacgac ctttgcccct aatgcaatgg
     8701 cgcgctccag ttcctcttca ttactgactt cggtcagcac ccccatctcc agactgtgag
     8761 cgacggctgc aagctggcga tattggtcgt catccagtac tgaaagcatt aataagcagg
     8821 catcggcctg gtaatagcgc gccagataga tctggtaagg gtcgataatg aagtctttac
     8881 ataaaatcgg ctgcggggcg atttggctga cgatgaggag gaaatcaaag ctcccctgaa
     8941 aatatttctc atcagtcagc accgaaattg ccgaagcgta atgtttataa atggcggcaa
     9001 tgcgtgccgg atcgaaatca tcacggatca cgccttttga cggcgacgct tttttacact
     9061 ccagaataaa cgccgtgcgt gcaccctgta gcgcatcata aaaatgtcgt gtgctcggct
     9121 gaacctcatt ctgaaaactg gccagcggtt gctgctgttt gcgggtttct acccaaatcg
     9181 ccttgtctgc gacgattttc gctaaaacgg tttgcatcat ttaccctcgt gccgccagcg
     9241 cggtaactct gtcgtaagcg gaaccactgc gcagtacctc aagaacggtt tgcgcattgg
     9301 cttgcagatc ttcatggcca tgcaggcgca ttaacatggc gacgttcgca gcgacggctg
     9361 cttcatgggc ggcgtcgcct ttaccttgta acaagcgtgt taaaatgtca cggttttctt
     9421 caggtgttcc gcctgccagt tgctcctggt ggtagggagt caggccaaaa tcttcagcgg
     9481 tcaattgata gctcttaatt ttgccgtcat gcagctcggc aacgattgtc ggcgcgtgta
     9541 atgaaacttc atccatcccg ccgctgtgca ccaccgccgc gcgttgatac cccagcacgc
     9601 gcaaggtttc ggcaatcggc agcaccagtt ccggactata aacaccaatt aacgccagcg
     9661 gcggatgcgc cgggttaatc aatggcccca gcacattgaa cagggtgcgg gttttcagtt
     9721 gctggcgaac cggcatcgcg tggcggaatc cggtgtgata tttcggcgca aagaggaaac
     9781 atacgcctaa ctcgtccagc gcctggcgcg atttattggc gttcatatca agattaatac
     9841 cgaatgccgc cagcagatcc gacgagccgg atttactgga gacgctgcgg ttaccgtgtt
     9901 tcgccacttt cagcccacag gccgcggcga
//
LOCUS       DADLTY010000153         9925 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.154, whole genome shotgun sequence.
ACCESSION   DADLTY010000153 DADLTY010000000
VERSION     DADLTY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.154"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..336
                     /locus_tag="JN334_003275"
     CDS             <1..336
                     /locus_tag="JN334_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439317.1"
                     /translation="SSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLNA
                     RNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYNI
                     DPLCECFKY"
     gene            494..1639
                     /locus_tag="JN334_003276"
     CDS             494..1639
                     /locus_tag="JN334_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439318.1"
                     /translation="MCERMKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSA
                     KDVGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPK
                     NNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREECKELPQSVEYVPLACEAH
                     SHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQS
                     DISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGC
                     KSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLF
                     DIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2318..2536
                     /locus_tag="JN334_003277"
     CDS             2318..2536
                     /locus_tag="JN334_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HAY7439319.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2754..2996
                     /locus_tag="JN334_003278"
     CDS             2754..2996
                     /locus_tag="JN334_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439320.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3326..4255)
                     /gene="fdhE"
                     /locus_tag="JN334_003279"
     CDS             complement(3326..4255)
                     /gene="fdhE"
                     /locus_tag="JN334_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HAY7439321.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4252..4887)
                     /gene="fdoI"
                     /locus_tag="JN334_003280"
     CDS             complement(4252..4887)
                     /gene="fdoI"
                     /locus_tag="JN334_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY7439322.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4884..5786)
                     /gene="fdxH"
                     /locus_tag="JN334_003281"
     CDS             complement(4884..5786)
                     /gene="fdxH"
                     /locus_tag="JN334_003281"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY7439323.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5799..8849)
                     /gene="fdnG"
                     /locus_tag="JN334_003282"
     CDS             complement(5799..8849)
                     /gene="fdnG"
                     /locus_tag="JN334_003282"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8262..8264),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY7439324.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9043..9876
                     /gene="fdhD"
                     /locus_tag="JN334_003283"
     CDS             9043..9876
                     /gene="fdhD"
                     /locus_tag="JN334_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAY7439325.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2490 a         2395 c         2542 g         2498 t
ORIGIN      
        1 tcgagtaata atgaatatga cctgtttaaa aatagagaaa cctcactgtg gaaacgcaaa
       61 gtaccagaaa gcgcagcgct ggcaatgacg ggaacagtat ataaattatt tggtaatcgc
      121 ttactcaacg cccgcaatca gttagataaa aatctcctcg aaatggctat gcaaatggtt
      181 tatggcaaag atatggtgac aaagtccggg gatatattga ttgatataca attaaataag
      241 gatggcagtc tccagagtaa gcactacaca cttgatgtag gtgaagttat aaacagttat
      301 aatattgacc ctctttgtga atgcttcaag tattaatcaa acttacttac atgataaaaa
      361 tcctggtgac gttattagcc tgtatggcac tttagataac attccccttg tagttaagga
      421 atgcacgggt aaaaatcatc aaattaatgt taaagccaaa ttgccacaag aacgaagtga
      481 aaaagttgag gatatgtgtg aaagaatgaa aggtggcatc tctatgttta accacaccac
      541 aaaaacagct gataatatag agcataattt gcagctaagt tttctcgtgg gttcacgtcc
      601 gtcgatcacc aataaatact cagcaaaaga tgtaggtcca gatatacttg cattgcccgt
      661 caacgtatat catgcggatt ttaaaattaa aatcaataat gcagtggaga aggcttcatc
      721 aattgtgtta tgtgctgtgc aaaaaaatct ccatgcagca aggaaggttc ttagcgggat
      781 aataggaaat gtgccaaaaa ataataaacc aaaaacaatt atttttgatt tgcgttttct
      841 tagtgcaatt aagcagaaag tttttttggg cagtgaaaaa ccgttgtttg taaagcatac
      901 cgcaatgatg agggaggaat gtaaggaatt accgcagtca gtggaatatg ttcctttagc
      961 ctgtgaagcg catagccatc gcagcgtcgc ccttgccaaa accgtggctg cgcccatcat
     1021 ggcgacaggt gtgggcttgg cattaatgta tgcaacatca tcaggaccgt attatgcgca
     1081 aagtctttct ggttcgccgg atatcgagat gataaaaaat cgcatggctg aattgtttaa
     1141 ccagtcggat atttctgtgc gcaacaaata tagcccggct tttgataaat ggaatgaact
     1201 ggtagataaa ttaaatcacg aaagaaatac agttccgttt gcgtgtttga ccaccatttt
     1261 agcgacggcg ataaatgaaa ctcttgaggg ggatacgaat gttgccgttg tgatggggtg
     1321 taaatctgcg aaagatcgca cgatatccat tgtccttggc aactcgatgt tgcaaacact
     1381 ccttgaaaaa cgcctcgccg atagtggaga aatagaaaat ctttttgatc aacagggata
     1441 ttttagttgc gattcattaa cacccgaaga attaatgatg ttaaaagacc tttttgatat
     1501 tcgagttctt catgtctcta acaaattcaa tgttggattg caaggtaata tcaacactga
     1561 tgttttacaa gattcgtttt ttaaaaatgt cgatttcatt catgaatata actcgtatac
     1621 cgtagggatg ggagtctgac gagtaaaact cattaggatt atcagaattc ggacaatgca
     1681 ttgcaaaaat gacatacaaa gagagatgtg tgcaggtact tagccgcgag gggcatggcg
     1741 aagccataca gaaagtacca gtattcagaa ctccagtgaa agcggcatag tggccgcctg
     1801 ctcaggagtt ttgctatgac ccgtaatcgt gctgaacgtc gccaccctat gatgcgatta
     1861 aaaaaactca tcgtcgcgct gatgattgac gtcaatgcta tcggatgatg ccgggggtgt
     1921 tccggaagga attccttgtt tcccgaactg ttaccaacaa aattataacg atgagcaagt
     1981 aaagaccggt tggtggcagt actgaccggt ctgtgatgaa agattaattt caccttaaat
     2041 cctggtgctg ttaggatgct gacgtttttc ggctacacca ttcagattat gtaaataaat
     2101 atccctgaat ataatatatt catagcaccc ataactcatc ctgggcgata actaaaccat
     2161 ttttgagtaa taaatgaaac aactgcaacg ctcttattat ttctggaaaa cgcctcgcaa
     2221 aaacaaaatg acatgcaaaa atccgcattt ttttaaatgc ttttccggcg taattttcgc
     2281 acatacatta atgagtaata tatgtaaggt ggtggtaatg gcgatgaata cggtttttct
     2341 tcatttatca gaagaggcaa ttaaacggct gaacaagctt cggggctggc gtaaggtttc
     2401 acgatctgca attttacgcg aagcggttga gcaatatctg gagcggcagc aatttccggt
     2461 gcgtaaagca aagggaggca ggcaaaaggg ggaggtagtt ggtgttgatg atcagtgtaa
     2521 ggagcataag gaatgaaaaa aatttttcat tcttgaatat aaaaaacaga tgcccttatt
     2581 ctggtattaa tacaaggctg ttttacttga acttataata actgcaactg ttacatcgta
     2641 tctggaaaac gcctcgcaaa aataaaaaat gatgcgtaaa tgagctttct ttaaagattc
     2701 ggcaagggta cttttctctc catagattca aaaatgatat tggcgagata tttatgaact
     2761 ctctggcagg tatagatatg ggcagaattt tactcgattt atcgaatgag gtgattaagc
     2821 aacttgatga tctcgaggtg cagcgtaatc ttcctcgtgc ggacctatta agggaagcgg
     2881 tagatcaata cctgataaat caatcgcaaa cagcaagaac cagtgttcct ggcatctggc
     2941 aagggtgtga ggaagatggt gtcgaatatc agcgtaagct gcgcgaggaa tggtaatggg
     3001 aaaaaatgat gtcaatcaga ttgctgacaa cgtgcgcgtt gttcatgccg gatgcggcgt
     3061 gaacgcctta tccggcctac aaagtcatgc aaattcaata tattgaagtt tattggtagg
     3121 cctgataagc gcagcgcatc aggcaatcat acgcttgtca tcagtgacat acccataacc
     3181 ttatcgatgt gttggaggga agaacgaggc aaacagacaa ttgcggcatg atcttgaatg
     3241 ggtaacgcgg aatacgagag atggtgtcgt cacaccctat gagctgtaat ccgggcgacc
     3301 tgcgccaccc ggaaaacgtc agagattact ctccttcacc cggaaacagg aacgggttaa
     3361 tggaactacg ggcgtagcct tcttgctcca ttcgcgcgtc cagtaccaga gaggcgaggt
     3421 catctgccac ggcttcaact ttcggttctt tttcctgata aagaatcttc aggtaagtgc
     3481 cgcagtcatc gcagctttcg gctttaatcg cggcctgttc gtcatccagc gaccagtaat
     3541 gcagtttgcc gctctgctcg cagttgctgc attttacgcg cactacgtgc cattcggttt
     3601 cacacaggtt gcagtgcagg taacgcagac cctgagtggt gccaatttgc accatgctgg
     3661 acaccggcat agagccacat accgggcaat attgacgttg ttcgccgtat tcagcgcggg
     3721 ctttgccggg gatcagattg gccatctgcg cccagtagag cgacagcgca gcccagataa
     3781 acggcgcttt atcgctgctg acggacgaga aatcagaggc aaacagcgcg ctggccatat
     3841 cttccagctc ctgagtcgat gccttctcca gattctcaat cactgccagc gccgggccgc
     3901 tcatttcagg tttcagctca gcaattagcg ccatcagcag cttttgccag tgcttatcac
     3961 gcggcagaac gtgaatatcc agcgggggct tgccctgtgc gctggcttct ttaatgcgcg
     4021 cggtcagatc catctccagc ggatggtcgt acagcaccac tttctgggcg tgggcgataa
     4081 gcgcagcaaa gcgcaggtaa tcacccagcg gattattttc tgccagctcg cgcagacgct
     4141 cggcgcggcg gttgtataaa ttcttgagcc gagggaacaa taacggcgga atcatatccg
     4201 ccgtacgttt ctcgctcgaa cccagctcat cttgcgggat tatgcgaata ctcattcagc
     4261 tttcttttcc gttgtcttgc ggacctcacg gtaccagcgc gggtgatgtt tcttcgccca
     4321 ggcgctggta acccatcctt ccaccatggc ggtaatcgtg cctttcaccc aaagggcggc
     4381 gtagatatgc accatgataa ccacaattaa cgccactgcg gcaaatgaat gcagcattaa
     4441 cgcgaatcgg atcaccggga ttgagaaagc aggcgcaaaa taaggacgcc agataatcac
     4501 gccgctcacc agcagcagaa ccaggaaaat aatcgccgcc cagaaaacgc atttctgacc
     4561 gaagttataa cgcccggtgt cacctacttc ctcgttgacg acgatcttac gaatattctt
     4621 cgcccaaaag atatcatccc gattgattag gttttgatgc cagtaacgga aaaacatgat
     4681 gatgaacgag gcaaacataa ccacgccgac aaacgggtgc agaattcgcg ccagctgcgg
     4741 tgtgcccatg atttgcatca accagttgaa ggacgggaac aaaaagccca gcccgctcac
     4801 cgccgccagg atgaagcaga aggcggtgat ccagtggttg atacgttccg gcgcggtgta
     4861 gcgcacgatg gtgtcacgtc gtttcatttg cgctcctcgt ctttctcttc gtgcagatta
     4921 ttctcttcct catccgcacg gttcggaccg acacccacgt agtggaagat actggccgca
     4981 aaggtagccg caaagccaac agccgcgagc ggtttccaga tgcctttcca gaatttcacg
     5041 gtttcgctga tttccgggtt ctccggcaag ccatgataca gatttggctt gtcagcatgg
     5101 tgcagcacgt acatgacgtg tgtaccaccg acgcctgccg gatcgtacag acccgcattg
     5161 tcgtaaccgc gggttttcag ctcagcaacg cgctcgctcg ccagcgtttt catcgactct
     5221 ttcgtgccga agtgaatcgc gcccgttggg caggtcttca cgcaggctgg ttcttgccca
     5281 accaccacgc ggtcaacgca cagcgtacat ttgtagacgc ggttgtcttc cgggttgagg
     5341 cgcggaatgt cgaacggaca gcccgcaatg caataaccgc agccaatgca ctgctcggac
     5401 tggaagtcga cgataccgtt ggcatactga ataattgcac cttccgccgg gcacgctttc
     5461 aggcagcctg gatcggaaca gtgcatacag ccgtctttgc ggatcagcca ttccagtttg
     5521 tcgttctgct ccacttccga gaagcgcatt accgtccacg atttggcgct taaatcattg
     5581 gggttgtcgt acaccccaat gttattgccg acggtatcgc gaatgtcgtt ccactctgaa
     5641 cacgccacct gacaggcttt acagccgata caggtggtaa cgtcgatgag tttcgccact
     5701 tcttcctgga agtcccgcgc ctgaggcgcg ggggtcagac cgttagtcgc ggaacgacga
     5761 atgatatctt gagattgata agccatatgt cgtctccgtt acaccttttc cacattcaca
     5821 aggaaggact taaactccgg cgtctgcgtg ttcgcatcac caacgaatgg cgttaaagta
     5881 ttggcaataa agcctttttt agctacgcct tcatagcccc agtgaatcgg aataccgatg
     5941 gtatcgatat ctttgccgtt cgctttcagc gtgcgaatac gtttggtcac caccgctttg
     6001 gctttgatat agccacggtt ggaggagact ttcacggtat cgccctgggc aatgccaagt
     6061 ttattcgcca gcgactcccc gatttccaca aactgctctg gctgcaaaat cgcgttcaac
     6121 agcgcgtgtt tggtccagta gtggaagtgc tcggtcagac gataggtggt tccgacatac
     6181 gggaacttat cggctttacc caatgcttcg gcgtcgtctt taaagatacg tgcagctggg
     6241 ttcgagataa cgtttggatg cagcgggtta gttcccagcg gcgtttcaaa cggctcgtag
     6301 tgttccggga acggaccttc cgccatctta tcgagggcaa acagacgccc catgccttcc
     6361 tgctgcatga taaacggccc gacgccgcta cccggaggcg ctgcgctgta gtccggaata
     6421 tcccagccgg tccacttagt gccgtcccat ttcagcaact gacgcttcgg atcccacggg
     6481 ttaccctgcg gatctgcgga ggcgcggtta tacagaatgc ggcggttaag cggccatgcc
     6541 catgcccagc ccagcgtgtt accgaggcca gacggatcgg cgttatcacg acgcgccatc
     6601 tggttgcctt ccggcgtcca gctaccggcg aaaatccagc agccacagga ggttgtaccg
     6661 tcatcgcgca attgggcgaa cgagctaagt tgttggcctt tcttgacgat aaccgcaccg
     6721 gttgccggat cggtaatatc ggccagcgcc ttaccgttgc tctccatcgc tacttcttcc
     6781 gacttcggct catgcggaat ggcgtagttc caggtcatgt tcagcacctg atccgggttc
     6841 gcgccaccct gttcggcata catcttgcgc aagcgcagga agataccgga gaggatctca
     6901 ccgtcagtca gcgcaatccc cggggcgtcc gcacctttcc agtgccactg caaccagcga
     6961 cctgagttaa cgattgaacc gttctcttct gcgaagcagg tcgatggcag acggaacact
     7021 tcggtctgga tcttcgacga gtcaacttcg ttcagctcac cgtggttctg ccagaagtta
     7081 gaggtttcag tgttcagtgg gtcgatggtg acgaggaact tcagtttcga cagacaaccg
     7141 atcactttgt ttttgttcgg gaatgaggca acagggttaa agccctggca gatatagcca
     7201 ttgaccttgc cctctttcat catctcgaag tattgcagga cgtcgtagcc tttatcccac
     7261 ttcggcaacc agtcaaagcc ccagctattt tccgccgtcg ctttatcacc aaagaaggcc
     7321 ttcatcatag agacgaagaa tttcgggtag ttgccccagt agtttacctg gccttccagc
     7381 agcggttttg gcgtgttggc ggtaaggtag gtttgcagat cggtctgctt ctcgcttggc
     7441 agcgtcatgt aacctggcag actctgcgac agtagcccca ggtccgtcag cccctgaata
     7501 ttggagtgac cgcgcagggc gttaacgccg ccgccggcca tccccatatt gccgagcagc
     7561 agctggatca tcgccatcgt acgaatgttt tgcgcaccaa cggagtgttg cgtccagccg
     7621 agggcataca ggaacgaggc agttttatcg tgagcactgg tttctgcgat gtattcgcag
     7681 actttcagga acgcgtcttt tggcgtacca cagatgtttt caaccacatc tggcgtgtag
     7741 cgggaaacgt gctgtttcag caagttccac acgcagcgcg gatgttgcag cgtggtatcg
     7801 cgtttggcga agccgttttc gtccagttca taagtccagg aggatttatc gtacttgcgt
     7861 ttttccgcgt cgtagccggt gaacaggcca tcttcaaagc cgtaatcctc acgcacgatc
     7921 aggctggcgt tggtataggc ttcggtgtat tcgtggttga atttttcatt gttcagcagg
     7981 tacagcaata cgcctgacag gaaagcaatg tcagtaccgg aacgaatagg ggcatagtag
     8041 tcagctaccg ccgccgtacg cgtaaagcga ggatcgatca caatcagctt cgcgccgttg
     8101 tgaattttgg cttccatcgc ccagcggaac ccgaccgggt gagcttcagc ggcgttaccg
     8161 cccatcacca cgacgaggtt ggcgttcttg atgtcgaccc agtggttggt catcgcaccg
     8221 cgaccaaatg ttggagcaag acttgctacc gttggtccgt gtcagacacg cgcctggttg
     8281 tcgaccgcga gcatacccag cgcgcgggag aatttttgcg ttaaatagcc ggtttcgtta
     8341 ctcgacgcgg aagcacacag catcccggtg gagagccagc ggttaacagt cacgccttcg
     8401 gcgttttgcg caatgtagtt agcatcgcgg tcttctttca tcagtttggc gatgcgatca
     8461 aacgcctctt cccaactgat ttgctgccat ttatcagaac ctggcgcacg gtattccgga
     8521 aacttcagac ggctttcgga gtggatgaaa tccaccaggc cagcgccttt cggacaaagt
     8581 gcaccgcggt tgaccgggtg atccggatcg ccttcgatat ggaagataga tgctttggcg
     8641 ttttttgctc cgtcaccgag gctgtacatc aacagcccac agcctacgga acaataggtg
     8701 caggtattac gggtttcgcg ggtgcgcagc agtttatact gccgggtttc cgcgagtgct
     8761 acgctgggtg caaaacccag tgccgctgcc gtggtgcctg ccataccgcc agcgcagatc
     8821 ttaaagaact gccttctgct gacctgcatg gattgctcct tgtttcgaca ttgtcacgtc
     8881 ccatttacat tcgcttgctg cgtgtgcagg gagtgggagt tatttttctt tgcggaaggg
     8941 gccgcaaagg tccagaattg gctcaatttc cctccatcta ggaagggttt gtaacagaat
     9001 accataatgt tggtgtgtat gttcttatct ggttaagaga aagtgaaaaa aacacagcga
     9061 aaagaaatcg aaaatgtgac aaatatcaca ggtgttcgtc aaattgagtt atggcggcgt
     9121 gatgatttac aacacccacg gttagatgag gtcgcggaag aagttcccgt tgcgctggtc
     9181 tacaacggca tttcgcatgt ggtgatgatg gcgtcgccca aagaccttga gtactttgcg
     9241 ctcggttttt cgctttccga ggggataata gaaagtccgc gcgatatctt cggcatggat
     9301 gtcgttcctt cctgtaatgg tcttgaagta caaattgagc tttccagccg ccgctttatg
     9361 gggttgaagg agcgccgccg ggcgctggcg ggacgtacgg gctgtggcgt atgcggcgtg
     9421 gagcaactta atgacatcgg aaaaccggtg cagccgctac cgttcaccca gacgtttgat
     9481 ctcaacaaac tggatgatgc attacgtcat ctcaacgatt ttcagccagt ggggcgactg
     9541 actggttgta ctcacgccgc tgcctggatg ttgccatctg gcgaactggt cggcgggcat
     9601 gaagacgtgg gtcgccatgt ggcgctggac aaactgttag gccgccggtc acaagaaggg
     9661 gaaagctggc agcaaggtgc ggtactggtt tccagtcgtg ccagttatga aatggtgcaa
     9721 aagtcggcga tgtgcggcgt agagattttg tttgcggtgt ctgccgcgac cacgcttgct
     9781 gtagaagtgg ctgagcgctg taatctgaca ctggtaggtt tttgtaaacc gggtcgggca
     9841 acggtttata cccatccgca gcgtttgagc aattaattaa aataagtcct gcgatatata
     9901 ttaaattgca ggacttattc atttc
//
LOCUS       DADLTY010000154         9905 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.155, whole genome shotgun sequence.
ACCESSION   DADLTY010000154 DADLTY010000000
VERSION     DADLTY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9905
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.155"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(160..900)
                     /gene="pflA"
                     /locus_tag="JN334_003284"
     CDS             complement(160..900)
                     /gene="pflA"
                     /locus_tag="JN334_003284"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAY7439326.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="JN334_003285"
     CDS             complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="JN334_003285"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY7439327.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3429..4286)
                     /gene="focA"
                     /locus_tag="JN334_003286"
     CDS             complement(3429..4286)
                     /gene="focA"
                     /locus_tag="JN334_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HAY7439328.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4692..6452)
                     /locus_tag="JN334_003287"
     CDS             complement(4692..6452)
                     /locus_tag="JN334_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HAY7439329.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6695..7208
                     /locus_tag="JN334_003288"
                     /pseudo
     CDS             6695..7208
                     /locus_tag="JN334_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313709.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7407..8495
                     /gene="serC"
                     /locus_tag="JN334_003289"
     CDS             7407..8495
                     /gene="serC"
                     /locus_tag="JN334_003289"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAY7439330.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8566..9849
                     /gene="aroA"
                     /locus_tag="JN334_003290"
     CDS             8566..9849
                     /gene="aroA"
                     /locus_tag="JN334_003290"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAY7439331.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2520 a         2368 c         2483 g         2534 t
ORIGIN      
        1 ttcgatgggg aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat
       61 atggtagatg cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa
      121 aaagcggcaa ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc
      181 gtactgctca agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc
      241 atcgagtttg tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg
      301 gagaagttcg attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc
      361 cgaatcgtca tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt
      421 tttgttcgcc agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt
      481 ttggtggatc tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag
      541 cagttcatca atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg
      601 aatgccttct tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc
      661 ttcaccgccg gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac
      721 cacttccttc atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc
      781 gcggttatga caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat
      841 acctgggccg tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat
      901 tgcggtgttt ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta
      961 cgtcgagtct gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt
     1021 gctaaaaaag gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca
     1081 gtcaaatcta attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct
     1141 ttagtcagcg agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat
     1201 ttttccgggt tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg
     1261 tgctgaccac cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg
     1321 ttggtcttac gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag
     1381 gagataccat ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca
     1441 gcacctttct ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca
     1501 cgacgaccgt ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc
     1561 agaacagact gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg
     1621 aaacgttcta ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc
     1681 gggtattcgc cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta
     1741 actttcgcat atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca
     1801 cacgccatgg tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg
     1861 tacttgtcgt gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag
     1921 tccatgaagt gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc
     1981 ggttcagact tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg
     2041 tacagcatgg ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg
     2101 atcatcgggc ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc
     2161 aggtcatcgt tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc
     2221 ttgaagttca gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc
     2281 cccatagtgt acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca
     2341 tcgaggccca taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca
     2401 tcgtattccg gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc
     2461 atttcctgcg cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca
     2521 tccaggaagg tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca
     2581 gccaggtagc cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag
     2641 atgtcgtagc cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca
     2701 gcgatttctt cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc
     2761 tgtagagagg tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca
     2821 acgcgacggt agtcaccgat gatacggcca cggccataag catctggcag accggtcaga
     2881 acaccggatt tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg
     2941 tgagttttac ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac
     3001 gctttgcagg aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc
     3061 ggagcttcag tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg
     3121 tgagaggtga tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt
     3181 tccagtttaa caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca
     3241 gccaggaagg actcgtcacc ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg
     3301 tttacttcat tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca
     3361 ttaagctcgg acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc
     3421 gacaaccatt aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc
     3481 aacaaaccac cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag
     3541 ttcatcacgg tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc
     3601 ggagatgcga agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg
     3661 tgctcaaaac cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc
     3721 atcaggctgc ggccagaata actcatccat actgccagac ataccatcag gtttgccagg
     3781 ataccaagac agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg
     3841 acgtttagtc cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt
     3901 acaaacagca gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac
     3961 tgacaccagg tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag
     4021 agatcggctc cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc
     4081 agttttgcca tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg
     4141 aatgcgattg agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt
     4201 ttcgttgctt tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa
     4261 agatcaaaag ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat
     4321 actaacaaag cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc
     4381 ccgtaactcg catggttttt atgcaaatac ggagtaacta tttgattatc caaataaaaa
     4441 taaattttaa aaattaacaa atgagttgaa ttttttccgc atcctccgct aaaacagtta
     4501 attaaaaggg agcatcaggc gaataaagta acaatatcga tcgtatttat taaatacaaa
     4561 ttaccgatat ttaaccttat aattacaatt attttattaa tgcaaatata tgtaaagcgg
     4621 ggcattaaaa aaacgccccg taatataact cagactaatc attaagccta cattgcgtag
     4681 gctatttgat tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc
     4741 ttcaacaacg actgatgtgc agcaaacgcg tgcagatcgc tatctaccgg ttgcaggccg
     4801 taaaacgccg cttcgccgct cattgccgca ctggcggctt ctacggcatc tgcgccgtac
     4861 atgcgaacaa acgcattcag atattgcagc ggctggcgat cttcttcctg tgccagtaat
     4921 aacaacgttt gcaggcagcg ataatagttg gcgcgttccg gactaaatac tgatgagtta
     4981 aactccatcg tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc
     5041 agcatggctt ttaattcacc gatacgcagg gtgtaccaac cgttatccga cccggtcgcc
     5101 agacccaaca gctcacgcac gcgggtaaag tcatcaaaac cttcttcatc cagttgctcg
     5161 atgaggttca ggtaatcttc tttttcccac tcgctgcctg gtagcgaaag aatcgtttca
     5221 cgtaaatggc tgcccatact gttattcgcg agccacagat cttcagccgg ataaatatcg
     5281 gacatgccag gcacgataat acggcaagca taaacgccca gatgctcgta atcggcaata
     5341 taaacttctt tatcttcttt gttgaagata gccatcagcg tagcaaactc ttcttccgtg
     5401 gtgccggaga aattccagtc cacaaacgga taatcggcat cctgcttgaa caggtcccag
     5461 gagattaaac cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct
     5521 tcatcatcga aggttggcgg agtaaacaca tccaaatctt tcaggccacg accttgcagc
     5581 agctcggtca cggtacgttc cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca
     5641 aagcaggtgc cgtttgcagg attaaacagt accacgcaaa tcaccggata ctggccgcca
     5701 agggaaccat catatgcgaa gatcgggaaa ccttctgttt ccagtgtttc gatcgcttca
     5761 accaccgctg ggtaacgcgc cagcacgtct gccgggattt ccggcaagct gatgctttca
     5821 gcaataatgc ggtttttcac gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct
     5881 tcgttgcggg tattgccagc ggacataccg ttagaaacgt acaggttacc aatgatattc
     5941 atcggaatat aaacggtctg attgtcggac tggcgcgtaa acggcagacc gcaaatacca
     6001 cgatcttcgt taccggattg taggtcaatc agcatactgc cggtcaattc attctccgga
     6061 tcgtaaaatg cgcgcagacg gtcatcgagc agcccttctg gcacatcgtc attttcgctc
     6121 agtgggaacc atttttcgtt gggataatgc acaaatggac cgttggcgat ggtttcgccc
     6181 agccagaaat cagcaaaaaa gtagttggtt gagagacgct cgaaatattc accgagtgca
     6241 gaagccagcg ccgctttctt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct
     6301 ttgtcgcgaa tatgtacaga ccagacgtta ggcacgggat tcagccagga ggcctcttca
     6361 atctgaaagc cgaggtctga aagtttttgc tggaagcgag cgatggaatc ttccagagcg
     6421 gcatctttgc cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg
     6481 cgcaataata cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt
     6541 gactatgctt ttagcggata aaccacgtta agagcataaa aatgaaggcg ttcgatctcc
     6601 accgtatggc atttgataaa gtgccttttg atttccttgg cgaagttgca ctacgtagtc
     6661 tttatacctt tgtactggtt ttttgttcct caaaatgacc ggaagacgcg gtgtgcgaca
     6721 gatgtcgctg tttgaagtgt taatcattct cacgctgggt tcagcggcgg gagatgtggc
     6781 gttttatgat gatgtgccga tggtgccggt acttatcgtc tttatcactc tggcgttgtt
     6841 ataccgcctg gtaatgtgat ggcgagctgg cctggtcgaa actcaataac tccaacatga
     6901 cggaatttga gttctttatg gagctacgat tgcgtggcgt ggagcagctg gggcaggtac
     6961 gtctggcgat tctcgaaacc aacgggcaaa tcagtgtcta tttctttgaa gatgacaagg
     7021 tgaaaccggg tttacttatt ttacccagtg attgtacgca gcgttacaaa gtggtgccgg
     7081 agtcggcgga ctatgcctgc attcgttgta gtgaaatcat tcatatgaac gcgggggaaa
     7141 aacaattatg tccgcgctgt gcaaatccag aatggacgaa ggcaagtcgg gcaaaacggg
     7201 tgacctgaca gtaaaaacat cggctttttg ctaataatcc gagagattct tttgtgtgat
     7261 gcaagccaca tttttgccct caacggtttt actcattgcg atgtgtgtca ctgaatgata
     7321 aaaccgatag ccacaggaat aatgtattac ctgtggtcgc aatcgattga ccgcgggtta
     7381 atagcaacgc aacgtggtga ggggaaatgg ctcaaatctt caattttagt tctggtccgg
     7441 caatgctacc ggcagaggtg cttaaacagg ctcaacagga actgcgcgac tggaacggtc
     7501 ttggtacgtc ggtgatggaa gtgagtcacc gtggcaaaga gttcattcag gttgcagagg
     7561 aagccgagaa ggattttcgc gatcttctta atgtcccctc caactacaag gtattattct
     7621 gccatggcgg tggtcgcggt cagtttgctg cggtaccgct gaatattctc ggtgataaaa
     7681 ccaccgcaga ttatgttgat gccggttact gggcggcaag tgccattaaa gaagcgaaaa
     7741 aatactgcac gcctaatgtc tttgacgcca aagtgactgt tgatggtctg cgcgcggtta
     7801 agccaatgcg tgaatggcaa ctctctgata atgcggctta tatgcattac tgcccgaatg
     7861 aaaccatcga cggtatcgcc atcgacgaga cgccagactt cggcaaagat gtggtggtcg
     7921 ccgccgactt ctcttcaacc attctttccc gtccgattga cgtcagccgt tatggtgtaa
     7981 tttacgctgg cgcgcagaaa aatatcggcc cggctggcct gacaatcgtc atcgttcgtg
     8041 aagatttgct gggcaaagcg aatatcgcgt gtccgtcgat tctggattat tccatcctca
     8101 acgataacgg ctccatgttt aacacgccgc cgacatttgc ctggtatcta tctggtctgg
     8161 tctttaaatg gctgaaagcg aacggcggtg tagctgaaat ggataaaatc aatcagcaaa
     8221 aagcagaact gctatatggg gtgattgata acagcgattt ctaccgcaat gacgtggcga
     8281 aagctaaccg ttcgcggatg aacgtgccgt tccagttggc ggacagtgcg cttgacaaat
     8341 tgttccttga agagtctttt gctgcaggcc ttcatgcact gaaaggacac cgtgtggtcg
     8401 gcggaatgcg cgcgtctatt tataatgcca tgccgctgga aggcgttaaa gcgctgacag
     8461 acttcatggt tgagttcgaa cgccgtcacg gttaatgccg aaattttgct taatccccac
     8521 agccagcctg tggggttttt atttctgttg tagagagttg agttcatgga atccctgacg
     8581 ttacaaccca tcgctcgtgt cgatggcact attaatctgc ccggttccaa gagcgtttct
     8641 aaccgcgctt tattgcttgc ggcattagca cacggcaaaa cagtattaac caatctgctg
     8701 gatagcgacg acgtgcgcca tatgctgaat gcattaacag cgttagggtt aagctatacg
     8761 ctttcagccg atcgtacgcg ttgcgaaatt atcggtaacg gcggtccatt acacgcagaa
     8821 ggtgccctgg agttgttcct cggtaacgcc ggaacggcaa tgcgtccgct ggcggcagct
     8881 ctttgtctgg atagcaatga tattgtgctg accggtgagc cgcgtatgaa agagcgcccg
     8941 attggtcatc tggtggatgc gctgcgcctg ggcggggcga agatcactta cctggaacaa
     9001 gaaaattatc cgccgttgcg tttacagggc ggctttactg gcggcaacgt tgacgttgat
     9061 ggctccgttt ccagccaatt cctcaccgca ctgttaatga ctgcgcctct tgcgccggaa
     9121 gatacggtga ttcgtattaa aggcgatctg gtttctaaac cttatatcga catcacactc
     9181 aatctgatga agacgtttgg tgttgaaatt gaaaatcagc actatcaaca atttgtcgta
     9241 aaaggcgggc agtcttatca gtctccgggt acttatttgg tcgaaggcga tgcatcttcg
     9301 gcttcttact tcctggcagc agcagcaatc aaaggcggca ctgtaaaagt gaccggtatt
     9361 ggacgtaaca gtatgcaggg tgatattcgc tttgctgatg tgctggaaaa aatgggcgcg
     9421 accatttgct ggggcgatga ttatatttcc tgcacgcgtg gtgaactgaa cgctattgat
     9481 atggatatga accatattcc tgatgcggcg atgaccattg ccacggcggc gttatttgca
     9541 aaaggcacca ccacgctgcg caatatctat aactggcgtg ttaaagagac cgatcgcctg
     9601 tttgcgatgg caacagaact gcgtaaagtc ggcgcggaag tggaagaggg gcacgattac
     9661 attcgtatca ctcctccgga aaaactgaac tttgccgaga tcgcgacata caatgatcac
     9721 cggatggcga tgtgtttctc gctggtggcg ttgtcagata caccagtgac gattcttgat
     9781 cccaaatgca cggccaaaac atttccggat tatttcgagc agctggcgcg gattagccag
     9841 gcagcctgaa tgaacaacgg gcaataaata gccaaatctt tctttatcaa aacgtcggca
     9901 cattg
//
LOCUS       DADLTY010000155         9773 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.156, whole genome shotgun sequence.
ACCESSION   DADLTY010000155 DADLTY010000000
VERSION     DADLTY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.156"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="JN334_003291"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="JN334_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAY7439332.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="JN334_003292"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="JN334_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HAY7439333.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="JN334_003293"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="JN334_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HAY7439334.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="JN334_003294"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="JN334_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HAY7439335.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="JN334_003295"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="JN334_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HAY7439336.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="JN334_003296"
     CDS             complement(6031..6360)
                     /locus_tag="JN334_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HAY7439337.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="JN334_003297"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="JN334_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAY7439338.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="JN334_003298"
     CDS             7998..8171
                     /locus_tag="JN334_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HAY7439339.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..9479)
                     /gene="kch"
                     /locus_tag="JN334_003299"
     CDS             complement(8226..9479)
                     /gene="kch"
                     /locus_tag="JN334_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HAY7439340.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2563 a         2286 c         2433 g         2491 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata
     9361 ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg
     9421 tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact
     9481 gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac
     9541 atgcagcgcg tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt
     9601 ttgagtgagc aactctgcgt caggaaaggg cttttttcct gaacatgctc acatcatata
     9661 aagaaataca gataaagtta ttatctgctt gtggtagtga atgcactgac cggctataag
     9721 gaaaggccaa acaagaacac ggttgcaaaa accgtgccct taaatattgg atc
//
LOCUS       DADLTY010000156         9761 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.157, whole genome shotgun sequence.
ACCESSION   DADLTY010000156 DADLTY010000000
VERSION     DADLTY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.157"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            383..1501
                     /locus_tag="JN334_003300"
     CDS             383..1501
                     /locus_tag="JN334_003300"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY7439341.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="JN334_003301"
     CDS             1567..1815
                     /locus_tag="JN334_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HAY7439342.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="JN334_003302"
     CDS             1880..2248
                     /locus_tag="JN334_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY7439343.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="JN334_003303"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="JN334_003303"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HAY7439344.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /locus_tag="JN334_003304"
     CDS             3103..4350
                     /locus_tag="JN334_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAY7439345.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="JN334_003305"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="JN334_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HAY7439346.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSQMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="JN334_003306"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="JN334_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HAY7439347.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="JN334_003307"
                     /pseudo
     CDS             complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="JN334_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2386 a         2705 c         2368 g         2302 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttattgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgttg gccttttggc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgcctata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctaatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc tgcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagccg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacggcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8461 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9541 acaccgggtc cataccctga attcgcgatt acgcgcaatc cccctgagaa ccgccatgaa
     9601 caagggtttc atgatttgtg attttagttt ggtacgcaat ttggtacaca acactatttt
     9661 cacttcagcg ggtagtcatc aaactcacca taacgagcat cgttgatgat gtatgtgatc
     9721 accacaaata tcacatctgg actagttccg tcttcatgcg c
//
LOCUS       DADLTY010000157         9637 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.158, whole genome shotgun sequence.
ACCESSION   DADLTY010000157 DADLTY010000000
VERSION     DADLTY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.158"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..781)
                     /gene="selB"
                     /locus_tag="JN334_003308"
     CDS             complement(<1..781)
                     /gene="selB"
                     /locus_tag="JN334_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAY7439348.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADV"
     gene            complement(778..2169)
                     /gene="selA"
                     /locus_tag="JN334_003309"
     CDS             complement(778..2169)
                     /gene="selA"
                     /locus_tag="JN334_003309"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAY7439349.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(2267..2875)
                     /locus_tag="JN334_003310"
     CDS             complement(2267..2875)
                     /locus_tag="JN334_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAY7439350.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(2946..4031)
                     /locus_tag="JN334_003311"
     CDS             complement(2946..4031)
                     /locus_tag="JN334_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7439351.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(4034..4396)
                     /locus_tag="JN334_003312"
     CDS             complement(4034..4396)
                     /locus_tag="JN334_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY7439352.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            4933..6846
                     /locus_tag="JN334_003313"
     CDS             4933..6846
                     /locus_tag="JN334_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAY7439353.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            6941..8089
                     /gene="mtlD"
                     /locus_tag="JN334_003314"
     CDS             6941..8089
                     /gene="mtlD"
                     /locus_tag="JN334_003314"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY7439354.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            8089..8676
                     /gene="mtlR"
                     /locus_tag="JN334_003315"
     CDS             8089..8676
                     /gene="mtlR"
                     /locus_tag="JN334_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HAY7439355.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(8688..8897)
                     /locus_tag="JN334_003316"
     CDS             complement(8688..8897)
                     /locus_tag="JN334_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439356.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            9181..9543
                     /locus_tag="JN334_003317"
     CDS             9181..9543
                     /locus_tag="JN334_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HAY7439357.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         2343 a         2581 c         2472 g         2241 t
ORIGIN      
        1 gcacatcggc aagcagccag tcgccacggt taatctgctc tttttccgca tcacccgcga
       61 tgtcaagcgc gatacgctgc ccggcattgg cggtttctgt tggctggttt tgcgcatgca
      121 gcgcacgtac acgcatcggt ttatttacac cagtcagcca gagtgaatcg cctaccttca
      181 cttccccgct taacgccgta ccggtgacga ccagcccggc accttttacg gtaaatgcgc
      241 ggtcaatcgc gaggcggaaa ctatgttggc tggcgtgctc gcgttccggc aactgaagca
      301 gatgctcgcg cagggcatca attccccgac cttcggttgc tgcggtgata aacagttttg
      361 cctcagcaaa accgtattcc cgcagaacct cctttacctg gcgttcaacc tcatcaacac
      421 gcgcttcgtc cacgcgatcg gctttggtca gcgccactgt cagcatcggg ttaccggtca
      481 gctgcaaaat cgccagatgc tcacgggtct gtgccatcac gccgtcatcg cacgccacca
      541 ccaacagcgc gtgatcgata ccaccaacgc ccgccagcat gttggaaaga aacttttcat
      601 gaccgggaac gtcgataaaa ccaggcacgc gaccatccgg ctgcggccag taggcatagc
      661 cgagatcgat ggtcatgccg cgcttttttt cttccggcag acggtcagca tttacgccag
      721 taatcgcctg caataaggtt gttttgccgt ggtcaacgtg tccggcagtc gcaataatca
      781 tttcaacaac atctccaaaa accgttgctc atcttcaagg cagcgtaaat ccagccacaa
      841 tcgtccgtca taaatacgac caatcaccgg cactggcaat tcacgccagc gggcggctaa
      901 tgactcaagg tggctaccgc gtccatcatg gggtgtaaac gttaatgccg cgctcggcag
      961 gcgatcaacc ggcagcgaac cactgccaat ctgcgaaaga catggcataa cctgtaccgc
     1021 aaactccgcg ccgtaatgtg cgacaagggg agcctgtaaa cgttgtgcct ggatttgaat
     1081 gacctctgcg ctgcgggtaa gcaggcgcag ggtcggtaat ttttcactca gagcttcagg
     1141 gtgtaaataa agacgcaacg tggcttccag cgccgcgagg gtcattttat ccgcgcgtaa
     1201 tgcacgcttc agcgggtggc tttgcaggcg ggcgatcatc tcttttttac caacgataat
     1261 cccggcctgc ggcccgccta acaacttgtc gccggagaag ctcaccagac tgacgcccgc
     1321 cgcaatcaac tcctgcggca ttggctcttt cggcaaaccg tactggctaa gatcgaccag
     1381 cgagccactg cctaaatcag tcaccactgg aacatccagc tctttgccga gcgccaccag
     1441 ttcagcttcg tctatcgctt tggtgaaccc ctgaatgctg tagttactgg tatgtacttt
     1501 catcaacagt gcggtatttt cattcaccgc ctgacgataa tcattcgcgt gcgtgcggtt
     1561 ggtggtccct acttcgtgta gggtgcagcc tgcctgacgc ataacatcgg gaatacgaaa
     1621 cgcgccgcca atctccacca gttcgccgcg agataccacc acctctttcc cgctggcagt
     1681 ggccgccaac atcaataaca ccgccgccgc attgttattg acgatacagg catcttccgc
     1741 ccccgtaata cggcacagca gctgcgccag cgcccgatcg cgatgtccgc gtccggcgtc
     1801 gtccagatcg tactcgaggg tcactggcga acgcatagcc tgcgcaacgg cttccaccgc
     1861 ggcttccgcc tgtaaagctc gcccaaggtt ggtatgcagc acggttcccg tcaggttgat
     1921 caccggacgc agcgcgctct gcgcctcttt cgtcaaccgg gcatcgactt cttgcgccca
     1981 gttttcacac cacgcaggca gcgtctggct gccacgaatc acttctcgcg cttcgtcgag
     2041 catctgacgc aacaattcca ccacgcgggt gtgaccataa gtatcacgca aagaaaggaa
     2101 ggagctatcg cgcaataagc gatcaatagc cggaagttga ctatagaggg aacgcgtttc
     2161 ggttgtcata ggaaacctgg ctgatcaagg ccctctcaca cggagaaggg cgtttaacat
     2221 aaccacggat tgtaacgtga gatgggtcag gaggacatat cgcgcatcaa gcctttggcg
     2281 gttcggtgcg agcaaaactt tcgcggctaa acaggttttc gaccagtttg actaagtgtg
     2341 ggcggtcgac acaccaacct ggcgcaacgc ggcggaaatt gagatatccg acagcacagg
     2401 caatggcgat agtcgccaga ttgaccgtat cggttttgag tgtgccatcg accagatatc
     2461 cttccagcac atccagactg cggttgattt tctcccgctg gcgtaacaat tcatcttcag
     2521 actgctgcgc cgccggacgc gcctgttcac gcaccgatac cagcccggca tccataatgc
     2581 catccgccag cgcctcaatt ttgcgcaccc gcaacgactc cagcggatcg cgcggcaaca
     2641 tcgccggagc gacattcatt aactcaatat attcagcgat gatcggcgaa tcaaaccagc
     2701 attcgccctc ttcggtcacc agcaccggca ctttccctaa cgggttaaat tgcgccacgc
     2761 cgttatccgc gttatagggc agttcattaa taaattcgaa agttatgccc ttttctaaca
     2821 acagaataaa aagtttgcgt acaaacgggc tggtgtagct accgacgagt ttcatgccga
     2881 gtcctttgtg cgaggaaaaa tatcagtatg gctcaggctc acgcaaaaca caggtgatat
     2941 aagtctcaat gatccaaata aagataatgc atccaactgg tcattcttag cagcactttc
     3001 cgcatcaccg aaacatggct gaaatggtcg gagtaaaccg ccacctgggc gtagatgccg
     3061 tcgggtaagg catcgatatc atcgttaggg tccagttcaa tggttcccag cacaccgtcc
     3121 gtgccgggaa cgaccgttaa tgattgcaat accccctgcg cctgataaga accgcctggc
     3181 acgacaggta aaatactagt caatttgccg tggaacacct gcccaggtag cgcgttaaac
     3241 accacttccg catcatcgcc aggtttcaga cgtaacagcg agttttgccg aaattgggcg
     3301 acaatttgcc gtttttgctc ggggatgaag accatcaccg gacgcagcgg caaggcagct
     3361 gcgtatgtac ctgggcggat cagtacctga gtgacgtagc cattgcttgg tgcgcgaatg
     3421 acagtctgct caagattata ttttgcttca gtaagttgcg ctcttaagct cacaatctgc
     3481 gattgctcgc cgttgaccat actgtcgagc tgactctgga tctgcgcctg ctccgccacc
     3541 gagcctttca ctaacgcatc ctgcgcgagg aaattttgcc gcgcatcgtc gatgtcacgt
     3601 tccgagaacg gattcaccgc cgcctggctg cctttcaggt aacgttgata atttttaaac
     3661 agacggtcgc gctccgctga aacctgggtg gtgttggcct gcgcttcagt gagctgcgca
     3721 cgcagcgtct ttatattatg cgtcgccgtc atcaggtcag cctgaagtct gtcaactcgc
     3781 gcctggtaac gaaccgggtc gagcttaaaa agcacctcgc ccttttgaat aagctgatta
     3841 ttcttgtcag tgacttcagt aacaattccc gtcacctgtg gcgtgatagg gatcgctatc
     3901 actgcctttt gcgcggtaaa agtgtaaggg tggttgtagt tcatcaacaa aatcaaacca
     3961 ctcaccagaa acacgcctcc cagcgccgcc gtcgccagcg ccacgtaagt taaaacaatc
     4021 aatagatcca taattagatc tccgggaacg tggaatagtc agtctttttc tcggcggcga
     4081 gttgatgctc cagccgggaa attcggtcag aaagtgcggc gatttccggt tcagtcgctt
     4141 tctcctgcga cgcaacatgt gactgcatac cccagccacg ctccggttga tagagcgtcg
     4201 cccagatcca cagaaacggc cagataacat gcagagtaaa caggctcacc cagcccgccg
     4261 tatgaatagc gtcggcatgg gggtgattgc gctttttggc aatcagataa gggatgtcat
     4321 gtatcgcgat gattccataa aaaatcacca gaaagacgaa gatcagcact cccaacgcga
     4381 aatagtttag aaacatatct gcctcggatt cacgtttatc agtgttgttt tttggctggc
     4441 agccagaagg gagtcaggct gatattttga caataatccg ggttcgcgat tctcgccata
     4501 acaccaaaga ataattttta gaggtgatga gttgcttagt tacataacga ttgtatgacg
     4561 aaggcataac atgctgtaga tcacatcagg tgaacgccgt aagaaaatat cttgtgattc
     4621 agatcacaaa gattcaacaa accatcaaaa caaaaatgtg acattactca catttaaatg
     4681 ccatttttag cgaaaatcac cgccttgttt cttttttata caggcgtttt gtgatgagcg
     4741 tcacgtcaat tacctctcta ccccctatat ttatgtgact gatatcacac aaaagcccgt
     4801 cgactggaca gttaaccgat tcagtgccag atttcgcagt atctacaagg tccggctacc
     4861 tctgccgcca cattaacaaa aaacctcggg cttccagcct gcgcgacagc aaacataaga
     4921 aggggtgttt ttatgtcatc cgatattaag atcaaagtgc aaagctttgg tcgtttcctc
     4981 agcaacatgg tgatgccaaa tatcggcgcg tttatcgcgt ggggtatcat caccgcgtta
     5041 tttattccaa cagggtggtt accgaacgag acgctggcga agctggtcgg gccgatgatc
     5101 acttatctcc taccgctgct gatcggttat accggtggta agctggtagg cggcaaacgt
     5161 ggtggcgtag tcggtgccat taccaccatg ggcgttatcg tcggcgcaga catgccgatg
     5221 ttcctcggtt ctatgattgc aggtccgctg ggcggctggt gcattaagca cttcgaccgc
     5281 tgggtagacg gtaagatcaa atccggtttt gagatgctgg tgaataactt ctccgcaggc
     5341 atcatcggga tgatcctcgc tattctggca ttcctcggca ttggcccgat tgttgaagcc
     5401 ctgtccaaaa tgctggctgc gggcgttaac ttcatggttg tccatgacat gctgccgctg
     5461 gcgtctatct ttgttgaacc ggcgaaaatc ctgttcctca acaacgccat taaccacggt
     5521 atcttctcgc cgctgggtat tcagcagtcc catgaactgg gtaaatcaat cttcttcctg
     5581 attgaagcta acccaggtcc aggtatgggc gtgctgctgg cgtacatgtt ctttggtcgt
     5641 ggtagcgcta aacagtctgc gggcggtgcg gcaatcatcc acttcctggg tggtatccac
     5701 gaaatctact tcccgtatgt gctgatgaat ccgcgtctga tcctcgcagt catcctcggc
     5761 ggtatgactg gcgtgttcac gctgactatc ctgggcggtg gtctggtttc tccggcatct
     5821 ccgggttcta tccttgctgt actggcgatg acaccaaaag gtgcttattt cgctaacatc
     5881 gcgggtgtgt gtgcggcgat ggctgtctcc ttcgttgtct ctgctatttt gctgaaaacc
     5941 agcaaagtga aagaagaaga tgatattgaa gcagcaactc gtcgtatgca ggacatgaaa
     6001 gctgagtcta aaggcacatc tccgctatct gcgggcgatg tgactaacga cctgagccac
     6061 gtacgtaaaa tcatcgttgc ctgtgacgcc ggtatgggtt caagtgcgat gggcgcaggc
     6121 gtgctgcgta agaaaattca ggatgcaggt ctgtcgcaga tttcggtcac taacagcgcg
     6181 atcaacaacc tgccgccaga tgtggacctc gtcatcactc accgtgacct gaccgaacgc
     6241 gctatgcgcc aggttccgca ggcacagcat atttcgctga ccaacttcct cgacagcggc
     6301 ctgtacacca gcctaaccga acgtctcgtt gctgcccagc gccatactga aaacgaagtg
     6361 aaagtgaaag acagtctgaa agacagcttt gacgattcca gtgctaacct gttcaaactg
     6421 ggcgcggaga acatcttcct cggtcgcaaa gcagcaacca aagaagaagc gattcgtttt
     6481 gctggcgagc agctggtgaa aggcggttac gttgagccgg aatacgttca ggcgatgctg
     6541 gatcgtgaaa aactgacccc gacttatctg ggtgagtcta tcgcggtgcc acacggtacg
     6601 gttgaagcga aagatcgcgt actgaaaacg ggcgtggtgt tctgccagta cccggaaggc
     6661 gtgcgcttcg gtgaagaaga agatgacatt gcccgtctgg tgattggtat tgcagcccgt
     6721 aacaacgagc acattcaggt tatcaccagc ctgaccaatg cactggatga tgagtccgtc
     6781 atcgagcgtc tggcacacac caccagcgtg gatgaagtgc tggaactgct ggcaggtcgt
     6841 aagtaatcca atcctaccct ctcctcacgg agagggtttg ggtgagggaa aagcctcacc
     6901 ccagccctct cgggtaaaaa cattgatgaa ggttaatact atgaaagcat tacattttgg
     6961 cgcaggtaat atcggtcgtg gctttatcgg taaactgctg gcagacgcgg gtatccaact
     7021 gacgtttgcc gatgtcaatc aggtggtact tgatgccctg aatgcccgtc atagctatca
     7081 ggttcatgtg gtcggtgaaa ccgagcaggt agataccgtt tccggcgtca atgctgtcag
     7141 cagcattggt gatgatgtcg ttgatctgat tgctcaggtc gatttagtca ctaccgccgt
     7201 tggcccggtt gtgctggaac gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa
     7261 agaacaaggt aatgaatccc cgctgaacat catcgcctgt gaaaacatgg tacgcggcac
     7321 cacgcagctg aaaggtcatg tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga
     7381 agagcacgtt ggctttgtcg attccgccgt tgaccgcatc gtaccgcctt cggcttcggc
     7441 aactaacgat ccgctggaag tgacggtaga aactttcagc gaatggattg tcgataaaac
     7501 gcagttcaaa ggcgcactgc cgaacatccc aggcatggag ttaaccgaca acctgatggc
     7561 atttgtcgaa cgtaaactct tcaccctgaa cacgggtcat gctataaccg cgtacctcgg
     7621 aaaactggcc ggtcatcaga ccattcgtga cgctattctc gacgagaaaa tccgcgcggt
     7681 ggtaaaaggt gcgatggaag aaagtggtgc ggtactgatc aagcgctacg gctttgacgc
     7741 agacaaacat gcggcgtaca tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa
     7801 agatgatgta gagcgcgtag gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct
     7861 gatcaagcca ctgctcggta cgctggaata cagccttccg cacaagaatc tgattcaggg
     7921 gattgctggt gcaatgcact tccgcagtga agatgatccg caggctcagg aactggcagc
     7981 actgatcgct gacaaaggtc cgcaggcggc gctggcacag atttccggtc ttgatgccaa
     8041 cagcgaggtt gtatccgagg cggtaaccgc ttataaagca atgcaataat ggtggaccag
     8101 gcgcaggaca ccctgcgccc gaataacaga ttgtcagata tgcaggcaac aatggaacaa
     8161 acccaggccg ttgaaaaccg tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc
     8221 tttctgatca ccgccgtcga gcttttgacc gaggcggtaa atcttctggt gcttcaggta
     8281 ttccgcaaag acgattacgc ggtgaagtat gctgtagaac cgttactcga cggcgacggt
     8341 ccgctgggcg atctttctgt gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc
     8401 caggaatacg aagatgcgga actgctgatg gcattgcgtg aagagctaaa tcacgacggc
     8461 aacgagtacg cctttaccga cgacgaaatc cttggaccct ttggtgaact gcactgcgtg
     8521 gcggcgttac caccgccgcc acagtttgaa ccagcagact ccagtttgta tgcaatgcaa
     8581 attcagcgct atcaacaggc tgtgcgatca acaatggtcc tttcactgac tgagctgatt
     8641 tccaaaatca gcttaaaaaa agcctttcaa aagtaagcaa cgtctgctta ctgcccctct
     8701 acctgcttcg gccgataaag ccgacgataa tactccagac gttgtagata taacggcacg
     8761 ctttcatcgg gtattccgga cggaatcgcg ttacggggag gaagtttttt cagatactcc
     8821 cggaacgcct ggcttgatgc catgaaatct acggctttat cgataagaag cggaacgttt
     8881 tcacctaatt tgcccatgat gatatctccg tattaccccg cgccgggaat gcgcggccgc
     8941 caattttagt ttaggttccc ggtaaagatg acattaagga aaacgtgctg aatcctcaat
     9001 aatcatccgc tgctgtttat tatctttttc tatagatttc ttaattaatc aacgaattgc
     9061 gtagtttttg agcatgttaa tgattacgtt gcagaactat tacaatgtca tcagttgtga
     9121 acaaagcacc tggtcgcgca tactaggggc tataaattta tctttatcag aagccatcac
     9181 atgaaagaag tcgaaaaaaa cgaaatcaag cgtctcagcg atcgcctgga cgccatccgc
     9241 caccagcagg ccgatctgtc gctggttgaa gccgcagaca aatatgccga gctggaaaaa
     9301 gagaaagcca cgctggaagc agaaattgct cgcctgcgtg aggttcatag ccaaaaactg
     9361 agtaaagaag cacagaagct gatgaagatg ccgttccagc gcgcaattac caaaaaagag
     9421 caggctgata tgggcaagct gaagaaaagt gttcgcggac tggtggttgt gcacccaatg
     9481 accgcgctgg ggcgcgaaat gggcctggaa gagatgactg gattttctaa gactacgttt
     9541 taagcccctc agacgactgg ttcgatcgta tcaacccgca tctggcttgc cttagctggt
     9601 caatgcgggg actctcctgt ctactcgcct aattacc
//
LOCUS       DADLTY010000158         9522 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.159, whole genome shotgun sequence.
ACCESSION   DADLTY010000158 DADLTY010000000
VERSION     DADLTY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.159"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..593)
                     /locus_tag="JN334_003318"
     CDS             complement(<1..593)
                     /locus_tag="JN334_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7439358.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQ"
     gene            complement(660..1385)
                     /gene="fimC"
                     /locus_tag="JN334_003319"
     CDS             complement(660..1385)
                     /gene="fimC"
                     /locus_tag="JN334_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY7439359.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1422..1961)
                     /locus_tag="JN334_003320"
     CDS             complement(1422..1961)
                     /locus_tag="JN334_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7439360.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2026..2574)
                     /locus_tag="JN334_003321"
     CDS             complement(2026..2574)
                     /locus_tag="JN334_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7439361.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3055..3651)
                     /gene="fimE"
                     /locus_tag="JN334_003322"
     CDS             complement(3055..3651)
                     /gene="fimE"
                     /locus_tag="JN334_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimE"
                     /protein_id="HAY7439362.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(4129..4731)
                     /gene="fimB"
                     /locus_tag="JN334_003323"
                     /pseudo
     CDS             complement(4129..4731)
                     /gene="fimB"
                     /locus_tag="JN334_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
     gene            6185..6901
                     /gene="nanC"
                     /locus_tag="JN334_003324"
     CDS             6185..6901
                     /gene="nanC"
                     /locus_tag="JN334_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HAY7439363.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6921..8027
                     /locus_tag="JN334_003325"
     CDS             6921..8027
                     /locus_tag="JN334_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HAY7439364.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            8092..9072
                     /locus_tag="JN334_003326"
     CDS             8092..9072
                     /locus_tag="JN334_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAY7439365.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2787 a         2077 c         1996 g         2662 t
ORIGIN      
        1 ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat accgggatcc
       61 cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg agggatcgtc
      121 aggttcagtc gctgctgacc aacatccaga tgcgcagtag cgtcctggac cattgtggtt
      181 aatggcacac aggcatcatc cgccagcaga ttcataccgg cgacagaagc cgtattcagc
      241 cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc
      301 gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat atcgacgcga
      361 tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc cacagcctgg
      421 ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg cagatgacaa aggtgcctgt
      481 gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac cagccaaacg atgcttacga
      541 atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga cattattcct
      601 ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt ccccctgcgt
      661 tattccatta cgcccgtcat tttgggggta agtgcgccat aatcatttat tgttcggtaa
      721 gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc
      781 aatgcatttt caagaacccg ggttccggca ttcaactctg ttaccgtcag gtaatagggt
      841 gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa atcttaattt ttctgcggcc
      901 tgatcgggtg gcaacgctaa tttagccggg cgatagtaca gtttaatgcg gctgataatt
      961 gcgagctgta gcatattctc agtcaatttt gatttatcca ttgacggaat cgctttaacg
     1021 ttcatccaga ataaactttc ccggtcctgt ggcaattggt tatttgttgc atcaagaata
     1081 cgtaaggtat tctctttttt tcccttcatc gcaaacagag gaggcgtcac gataaaacga
     1141 ccatccttta caccatcggc attttccacc catgattgaa ttaaataggt actattttca
     1201 tcattatttg tcacggcaag ttgcacttgt ttttgccctg ccggataaat tacgcgagtc
     1261 gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca tcattgccat gaacatcagg
     1321 atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattgac gtttttatta
     1381 ctcactgtcc tgttcctgtt atcacattat ctactgaaca attactgata ggttaaagag
     1441 aaccaggcct gggcattggc gatgccgcca gtaacccgac gcccggtagc acgatatttg
     1501 gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc tggaggacga
     1561 ttaatcggta cgaggtttcc ttcatcatca aacaacgcta caccaatatt ggaggctatc
     1621 cctggcccct ctcccacgga gagcacatcc ggatttttac catccgcgac accgtgaaac
     1681 gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat aacaaaaggc
     1741 accggtgcgc tatcttcccc tgccgcatga aaccggttac tgctgatttg ccccatattg
     1801 accgtcattt gtttatcacc ggcttcaatc cggcaagttt ccgcaataat gacgccctga
     1861 aattgcatat ttccgccggg cagcgtggta ttccatttat ttccggccag agcaaacatt
     1921 ggcagcaacg acgctaacag aaataatctc ttccttttca tcgttatttt tatcgcacaa
     1981 gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg gtaggttatt gatactgaac
     2041 cttgaaggtt gcatccgcat tagctgcacc cggggttgcc tcgccgattg cataataacg
     2101 cgcctggaac ggaatggtgt tggtaccgtt attcagggtt gtttgtgcac taaatgtcgc
     2161 accgtccagc gtcagagcat tgcctgttct gtctaggatc tgcacgccaa cgtttgttgc
     2221 actacctgca gcagaactct gcagagccag tacatcagta cgcgtagcat caattgccgt
     2281 acctaagaag gcaacagcgg cttttgtggc aacagtggta tcgcaatcat tcagctgaat
     2341 gttaaaacca acggcagagc tggttgctcc agcctgcttc aggctagcgg tacgaacctg
     2401 tcccaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg cgttaacaac
     2461 ttccccttta aagtgaatgg tcccaccatt taccgtcgta gtatcggcca gagccgctgc
     2521 ggaactgagg gacagagccg acagaacaac gattgccaga gttttaattt tcatgctgct
     2581 ttcctttaaa aaaactattt ctaaatcgac atgggcagtc gttctgtaca ctttgttttg
     2641 tcaacgagtt tgactgccaa cactgcacag ttttccccca aaagatgaaa catttggggc
     2701 cattttgact catagaggaa agcatcgcgc acaaactttt tcagtttatt tgttggctta
     2761 atattctatt attatcttta tttatagatt tttatattgc atgaggtggt ttttggagag
     2821 aagaatgagg aagatgcgtc gagccacaga aacgttagct ttacatatag cggaggtgat
     2881 gtgaaattaa tttacaagag aaataattta catatcaaat agttagatgc tttttgtcgt
     2941 tttttaatat ttttatgctt gagaaaaaat acgtaactta tttatgatat ggacagtttg
     3001 gccccaattg tcttgtgttt atttgttttt taactttatt atcaataagt taaatcaaac
     3061 ctcttctctt tttaattttt cgtttatgag attatttctt tcccataatc cggcaaaacg
     3121 agcagcatta ctggcggtat aacgcacagt atggcgaata tttcgatgcc cgagataatc
     3181 ctgaattaaa cgggtatctg caccacgctc cgccagttca taaccgcaag catgccttaa
     3241 catatgagga tgagtctgcg ttacggttcc agcttcaata ccggcatcgc gaataatgcg
     3301 ataggcctgc tggcgagaaa gccgactccc gcggcgagaa ataaatatag cgtcggtccg
     3361 gtcagcgcct ttccagttag cacgttcctg ggtccagcgt tccacggctt cacgctcatc
     3421 aaaacgtaac gggtgaacgg tagaaaatcc gttcttcagt cggcgaatat ttattcgacc
     3481 ttcattaagg tcaaggtcct gataatgcag atcaagcagt tcactaatac gcatcccatg
     3541 ccgatatgcc aacagaataa gacaataatc tctggctccc gttgccccgt aacaaaccgc
     3601 ctgcatcatg gcctgaactt ctttaccggt aagataacga cgtttactca caatagtagt
     3661 actcctaact gagatatatt tgaatgaata cctataggaa acctcaatcg gtcaaaatta
     3721 gcccaataga taaaaagata accacacacg aagttatgtg gctatcagtc aattacatta
     3781 aacatcatac aaaaataaaa tatcactgat gtgacaacat ctattttcat tgatttgatt
     3841 caatataaca atagattaca gaggattaac ttccagttac ttaaaaaata aagaaaaaag
     3901 tgtggaaaca ggagaattta agtgatgtgt aaacttatga atgattttgt ttatttattt
     3961 tgaagaacat aaatcattag attttgtatg ggtatggtta acttaatgtt tattatgata
     4021 attaagaatc acacttacaa taacatcaat taataaatgg ttaatcattt ttgatatatc
     4081 gtaagaataa tgtagttttt aacaccatcc ctggtatctc aactatctct ataaaacagc
     4141 atgacgctgt cgtcctctgg ctctatccca gatgccgtaa aaacgccctg cattgctggc
     4201 ggtataccag acagtatgac gaatattgcg atgcccaaga taatcctaga taagtcgcgt
     4261 atctattccc atattcgcca aagcaaaacc acacgaatag cgtaacatgt gcggatgaat
     4321 ctccagtgac aacccggcat taccaccaga agtcgagata atatggtaaa actgttgccg
     4381 agaaagcgga ttccccttac gtgataaaaa tacccactcg ctctcagcat gcggataaga
     4441 agtacggata ctcaaccagt tttttaaagc ctgaacttct ttgttcaata gcgggtgcgt
     4501 tgttgaaaag ccttttttta atcgatggat atatatacac tttgctttaa gatcaatatc
     4561 cgaaatcctc aatcgacaaa tttcactcgc ccggaaacca tgaataaaac aaagcaaaat
     4621 cagacaataa ttacgtgttg catgaggccc ggtatttgct gctttaagga gtgactcgat
     4681 ttcactatgg gtaaggaagt tccttttttt gttatcagcc ttattcctca tcgttttccc
     4741 ttataattac agacgcgcac tagctgtgct gggttagcaa taatccaaca tttttatcct
     4801 gattatgttt ataaaagcga acgtttgctt aataacttac gtaagtggac cagttcttct
     4861 gagcgaactt aaatggagta gcgatagtta cttataataa ctttgccata caaacgccat
     4921 gctcttgcat gctatgtacc tttatatatt tatcaatctg atacgaatct aataataacc
     4981 ctcatcatta atgcaactaa tcttatctat atatcatgtg atatgttctg taacaagtaa
     5041 aatcaacata aaaaattcag caaaatttaa tatttgttaa actagccgtc tcaaatatga
     5101 cattattagt taactgcatt tgccacaaaa tggctatttc atggaacgca gtaatttctt
     5161 agtggctatt atcatgctaa ttcttagatc cctagcgatt attcctgcct ggttcgtcag
     5221 cttaggccat gagattgtta cattcctttt tatcctggag atatggggga acgcaggcga
     5281 cgctttttca tctacatccg ttggtaaata tagctttggt cactacgact taagccttcc
     5341 tcaatcacag ctattcgccg ggcgatacgg caaacaaact aagtgaaaca tcatcctgaa
     5401 aacaccaaca tcaacaagcc tctccagatc gacatcagga gtgaccagtt acaagccaca
     5461 aaccaagaaa catatttcat gatgagaatt atgctcagcc tgatggcgag taaaccctta
     5521 ttttcaccac tctcactgag ttagcttgca aagctttctt tgtttagatt ctaaataaat
     5581 tatttataaa acgtatagtt aaaagaaaat ctttagtttt tgaagaccac cgcaagtgtt
     5641 cgtctggctt cacatggcat cttcttctta gaaaaagatc gacatatttt gtgacgcgaa
     5701 ttgcaaatct ggttttgttg tatggattgc gtgatttttg atctggtata acaggtataa
     5761 agatgcacca cgatagtcaa tgagactggg catctcgtaa tctatggcaa acatcacttc
     5821 agttctttca catcgggtga tgaaaatgca cttcagtctg aaaggaatat gaaaatgaga
     5881 tcaacagaca ctctatttta tgactctggg taaaatgggt tgagtaagtg atataactta
     5941 cgaatattca aatcaattaa aaatcagaag agattttata ctcaggtatt taatctggat
     6001 ctctgtttat ttaaataatg ttaaaagaga attttcacag gagaccttat acaaaaaata
     6061 taaaatacag ttacccgttg ccaaagacac tataagcctg gcaaaaaaat attacacaac
     6121 ataaatgcta attgtttatg cgggctttgt attgctttct gtatcctaca aatgagtgaa
     6181 atttatgaaa aaggctaaaa tactttctgg cgtattatta ctgtgctttt cgtccccatt
     6241 aatttctcag gctgcgacac tggacgtacg tggtggatat cgtagtggaa gccacgccta
     6301 tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa gcatggaaag
     6361 taatacctgg aataccattc atgataataa aaaggaaaat gccgcactca atgatgttca
     6421 ggttgaagtt aattacgcaa ttaaacttga tgatcaatgg acggtgcgcc cgggaatgtt
     6481 aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt cctgggacgc
     6541 gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt accgacaaca
     6601 agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat atgtcactta
     6661 ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca aacagaatga
     6721 ttatcgctat gcaaaccaca agaaatgggc gacggaaaat gcctttgttc tacaatacca
     6781 tatgacgccc gatattacgc catacataga atatgactac cttgaccgcc agggtgttta
     6841 caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat ttaaactgta
     6901 gtagacagga gacagtcaca atgaataaaa ctataatggc gcttgctatc atgatggctt
     6961 catttgccgc aaacgcgtct gtattaccgg aaactcctgt gccatttaaa agtggtaccg
     7021 gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtaca gcatggtaca
     7081 agctagatac acaggccaaa gataaaaaat ggactgcgtt agctgcattc cctggtggac
     7141 cgagagagca agcaacctct gcatttattg atggcaatct gtatgtgttt ggcggcattg
     7201 gcaaaaacag cgagggattg actcaggtat ttaatgacgt acacaaatac aaccccaaaa
     7261 ccaatagctg ggttaaattg atgtcgcacg cgccgatggg catggcgggt catgtgactt
     7321 ttgtacacaa cggcaaggct tatgttactg gcggtgttaa ccagaatatc ttcaatggct
     7381 attttgaaga tctcaacgag gctggaaaag attcaaccgc tatagataaa atcaacgccc
     7441 actattttga caaaaaagca gaagattatt tcttcaataa gtttctgttg tcttttgatc
     7501 cctcaacaca gcaatggagt tacgctggcg aatcgccctg gtacggaacg gctggtgcgg
     7561 cggttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa ccaggattgc
     7621 gaacggatgc cgtatttgaa cttgatttca ccggtaataa tttaaaatgg aataagcttg
     7681 atcccgtctc atcaccagat ggcgtcgctg gcggttttgc ggggataagc aatgattctc
     7741 ttatatttgc tggaggggcc ggattcaaag gttcacgaga aaattaccag aacggtaaga
     7801 actatgcgca tgaaggcctg aaaaaatcat atagcactga tattcatctt tggcataacg
     7861 ggaaatggga taaatcgggt gaattatcgc aaggtcgggc ctacggagta tcattgccct
     7921 ggaataatag tttattaatt attggcggtg aaactgcagg cggcaaagcg gtgacggatt
     7981 cagttttaat ttctgtgaag gataataaag tcacagtaca aaactaacgc ttcagggccc
     8041 cggtaaaggg gccctgctat caatttgtca tttgtattac gcgaaaatta tatgaacgca
     8101 ataatatcac ccgattatta ctatgttctt accgttgctg gtcagtctaa tgccatggcg
     8161 tatggcgaag gactgccatt accggacagg gaagatgcgc ctcattccag aattaaacaa
     8221 ttagcgagat ttgcgcatac gcatcccgga ggcccgccat gtcactttaa cgacattatt
     8281 ccactgactc actgcccaca cgatgttcag gatatgcagg gttatcacca tcctctggca
     8341 acgaatcatc aaacacagta cggcaccgtt ggccaggcac tgcatattgc acggaaatta
     8401 ctgcccttca ttcctgataa tgcagggatt cttatcgttc cgtgttgccg tggcggatcg
     8461 gcttttaccg cgggcagcga agggacatat tcagaacggc acggagccag ccatgatgct
     8521 tgtcgttggg gaacggatac tccgctgtac caggatttag tcagcagaac gcgagccgca
     8581 ctggcaaaaa atccgcagaa caaattcctc ggcgtatgct ggatgcaagg cgaatttgac
     8641 ttaatgacca gtgactacgc gtcacaccct caacacttta atcatatggt tgaagccttt
     8701 cgtagggatc taaaacaata ccattctcag cttaataata ttactgacgc gccgtggttt
     8761 tgcggcgata ccacctggta ctggaaagaa aatttcccgc attcgtatga agctatttat
     8821 ggcaattatc aaaataatgt tttagccaat attattttcg tcgacttcca gcaacaaggt
     8881 gaaagaggac tgacgaacgc gcctgatgaa gacccggacg atttaagcac gggatattac
     8941 ggttcagcgt accggtcacc ggagaactgg acgacggcac tgcgaagcag tcatttcagc
     9001 acggcagccc gtcgggggat tatttctgac agatttgtag aagcaatttt gcagttttgg
     9061 cgcgaaaggt gagcggggag tttattcatg ggggatgtcc ttgttcgttc tcagagtaga
     9121 caacaaaaaa caaatgagat caaggtaata tctcttcact tgcgggctcg aacagcaggc
     9181 aattgccaat ataataatct ggcacaaggg tgaaaaacgc gcaccgcata aaccgttgtt
     9241 attgcttaca gattagccgg atgccttaat agacatccgc gccttttcga ttatggctcg
     9301 gaaatctacg aacatctaca cagtttactg gaacgttttg ggccacagcg ttcgcagcac
     9361 cgtcctgata tgccgttctg gcgattataa gtcgacggat tctggcaaat agacgatgat
     9421 gatatgctta atgatgttca aagaaattag gccattatta tattattgct tgcattttct
     9481 ttcattccta aaagcacccg ccattaatcc caggcgcatt at
//
LOCUS       DADLTY010000159         9492 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.160, whole genome shotgun sequence.
ACCESSION   DADLTY010000159 DADLTY010000000
VERSION     DADLTY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.160"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..189)
                     /locus_tag="JN334_003327"
     CDS             complement(<1..189)
                     /locus_tag="JN334_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439366.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            709..1404
                     /gene="minC"
                     /locus_tag="JN334_003328"
     CDS             709..1404
                     /gene="minC"
                     /locus_tag="JN334_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAY7439367.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1428..2240
                     /gene="minD"
                     /locus_tag="JN334_003329"
     CDS             1428..2240
                     /gene="minD"
                     /locus_tag="JN334_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAY7439368.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2244..2510
                     /gene="minE"
                     /locus_tag="JN334_003330"
     CDS             2244..2510
                     /gene="minE"
                     /locus_tag="JN334_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAY7439369.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2882..3226)
                     /locus_tag="JN334_003331"
                     /pseudo
     CDS             complement(2882..3226)
                     /locus_tag="JN334_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3261..3380)
                     /locus_tag="JN334_003332"
     CDS             complement(3261..3380)
                     /locus_tag="JN334_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY7439370.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3341..3526)
                     /locus_tag="JN334_003333"
     CDS             complement(3341..3526)
                     /locus_tag="JN334_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439371.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3627..3800
                     /locus_tag="JN334_003334"
     CDS             3627..3800
                     /locus_tag="JN334_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439372.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3862..4146
                     /locus_tag="JN334_003335"
     CDS             3862..4146
                     /locus_tag="JN334_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HAY7439373.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVSAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            4150..4485
                     /locus_tag="JN334_003336"
     CDS             4150..4485
                     /locus_tag="JN334_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HAY7439374.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4542..6863)
                     /locus_tag="JN334_003337"
     CDS             complement(4542..6863)
                     /locus_tag="JN334_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7439375.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6980..7189)
                     /locus_tag="JN334_003338"
     CDS             complement(6980..7189)
                     /locus_tag="JN334_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439376.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7608..7844)
                     /locus_tag="JN334_003339"
     CDS             complement(7608..7844)
                     /locus_tag="JN334_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439377.1"
                     /translation="MFESFDRIAISSMNNSNNLDYFTLYIIFSIAFMLITLLVILIAK
                     PSTGLGEVLVTINLLNALVWLAINLVNRFKRKTR"
     gene            complement(7940..9463)
                     /locus_tag="JN334_003340"
     CDS             complement(7940..9463)
                     /locus_tag="JN334_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7439378.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2658 a         2205 c         2080 g         2549 t
ORIGIN      
        1 ggctttttcg ccgagatatt tccttgttaa ctctggggaa atgccatctg agttcacaca
       61 aatatacggg tcgcaaacaa cgccgcacgt cggagattga agtccgccag cgtcatgtcc
      121 agacgccatt gatgagaaca tcatccccac acctgacaat ccgatataaa taatacgcat
      181 catattcata gcacatcctg ataatccaac cgtacaacac ttatgctcat ttcgtcatca
      241 atccgtaatc aaaaccgctg acctggacga gttacgatga cctgacagaa gtatcagacg
      301 tcttctgcgg tttctatgat agagcaattc tgtggcggat attaagataa cgggtatctg
      361 ttctttaatg atgctgcaaa aaatggaaag ttaagaaagc ctggcgtggt gaaaaaatac
      421 acttgcctca atactggata tttaaacagg tctggagatg ctgtcacgat tagggtgcgg
      481 aaaaaggaag cgtaaataag aagaatcaaa agtacgacgg caatgggttg attgacaagg
      541 gtatttttta agctatgaat cagcgccatt tatcacagaa tagactttta ctctgaataa
      601 atgggagggt gacttgcctc aatataatcc agactataac atgccttata gtcttcggaa
      661 catcatcgcg cgctggcgat gattaatagc taattgagta aggccaggat gtcaaacact
      721 ccaatcgagc ttaaaggcag tagcttcact ttatctgtgg ttcatctgca tgaggcagaa
      781 cctaaggtta tccatcaggc gctgaaagac aaaatcgctc aggcccccac atttttaaaa
      841 catgcccccg ttgtactcaa cgtcagtgca ctggaagacc cggtaaactg gtcagcgatg
      901 cataaggcgg tttcggcaac cggtttgcgg gttattggcg taagtggctg caaagatgcg
      961 caacttaaag ccgaaattga aaaaatgggg ctgcctatcc tgacggaagg aaaagaaaaa
     1021 gcgccacgtc cagctcccgc accgcaggct ccagcgcaaa atacaatgcc ggtcacaaaa
     1081 acgcgtttaa tagatacccc ggtgcgttcc ggtcagcgta tttatgctcc acaatgtgat
     1141 ctgattgtta caagccacgt tagcgctggg gccgaattga ttgccgatgg gaacattcat
     1201 gtctatggca tgatgcgcgg tcgtgcgctg gcaggggcaa gtggtgaccg ggaaacgcaa
     1261 atattttgta cgaacctgat ggcggaattg gtgtccatcg caggtgaata ctggctgagt
     1321 gatcaaatcc cagcagaatt ttatggcaaa gcggcgcgac tgcagttagt cgaaaacgct
     1381 ttgaccgttc aaccgttaaa ttgatccctt tttaacaagg aatttctatg gcacgcatta
     1441 ttgttgttac ttcgggtaaa gggggtgttg gtaagacaac ctccagcgcg gccatcgcca
     1501 ctggtttggc ccagaaggga aagaaaactg tcgtgataga ttttgatatc ggcctgcgta
     1561 atctcgacct gattatgggt tgcgaacgcc gggtcgttta cgatttcgtc aacgtcattc
     1621 agggcgatgc aacgctaaat caggcgttaa ttaaagataa gcgtactgaa aatctctata
     1681 ttcttccggc atcgcaaaca cgcgataaag atgccctcac ccgtgaaggg gtcgccaaag
     1741 ttcttgatga tctgaaagcg atggattttg aatttatcgt ttgtgactcc ccggcaggga
     1801 ttgaaaccgg tgcgttaatg gcactctatt ttgcagacga agccattatt accaccaacc
     1861 cggaagtctc ctcagtacgc gactctgacc gtattttagg cattctggcg tcaaaatcac
     1921 gccgcgcaga aaatggcgaa gagcctatta aagagcacct gctgttaacg cgctataacc
     1981 caggccgcgt aagcagaggt gacatgctga gcatggaaga tgtgctggag atcctgcgca
     2041 tcaaactcgt cggcgtgatc ccagaggatc aatcagtatt gcgcgcctct aaccagggtg
     2101 aaccggtcat tctcgacatt aacgccgatg cgggtaaagc ctacgcagat accgtagaac
     2161 gtctgttggg agaagaacgt cctttccgct tcattgaaga agagaagaaa ggcttcctca
     2221 aacgcttgtt cggaggataa gttatggcat tactcgattt ctttctctcg cggaagaaga
     2281 acacagccaa cattgcaaaa gaacggctgc agattattgt tgctgaacgc cgtcgcagcg
     2341 atgcagaacc gcattatctg ccgcagttgc gtaaagatat tcttgaggtc atttgtaaat
     2401 acgtacaaat tgatcctgag atggtaaccg tacagcttga gcaaaaagat ggcgatattt
     2461 ctattcttga gctgaacgtg accttaccgg aagcagaaga gctgaaataa gcccgctgta
     2521 aaaacgcatt tatcttcaag gcagagtcat ttctgccttg agtttttcat ccctctcatc
     2581 cacgttgtgg taaagcggcg agtattcttg ctgatactcc tcattgctat ttccacctcc
     2641 ccctttacac cttaaggctg ttcccacgca cagaacaaaa tgtttatgcc tggttgagta
     2701 aataaccatg ttattagtgt gtatatactg ggagatgata accccgcaat ttcattattc
     2761 attgttctcc cataggatga ggtttcccgc cgtaactggc gggctttttt tgccccagaa
     2821 ttttctcttt caacatcctg taaacgaaaa ctgcgccgaa gcgcagttta atcgttgatg
     2881 gtcagaacga ccagttcaca ccagccaccg cgttccacgg ggactccaca ccggcaccat
     2941 ggctataccc caccccgaaa tgcccgctta acgtactgct gaatgaggct ttaatacctg
     3001 cctggtatat tccacgtctg cccgacaaat cattgacaaa attaccgtca ctattcactt
     3061 tcacccgatt atcatcgaca aattctttgc gcacagccgc cttcaggcag ggttcaattt
     3121 ccataccgtt ccccagacgc atgttgtaac tcagcgttgc acccagttca cgatatatac
     3181 tgcgggtatc gactgatttc gattccatgc cattggataa atgatagtag cgtctaagtg
     3241 acctaagctc tgctgcaaca tcagcccgca ggcaccagac caggggattc atcctgaaga
     3301 gacagcgcag gtgtattgtg ttcaccgctc accaaagaca tcatgatgaa atgatgatat
     3361 cccgcataag aaagaggcat tttttacacg cagtgcgctg aagtgtagtt ggataaaaaa
     3421 tcactctttt cagataatac tgtcgtacgc ttatctttag acaatgaggc cgtcggaaaa
     3481 ataaaatgac taatgtaatc gttgctgatg aatatcttct gctcacataa aaatcacaca
     3541 ataatattga taaatcagat tatcttacct ttacaacatt gcgttaacag tcaatgacac
     3601 ttcttgaata aaaatattgt caatcaatga gttattcctc atttaagatt attctcattc
     3661 acgtaaaaaa cataattcct atcatcacag ccacgctcat tttgaattat ctgaataata
     3721 gcgagcgttc tttagttaag cagatcttaa ttgaagacga aattattgtg tgtgcaactt
     3781 acttaatacc ggacatctaa gttgaagaaa aaaatacttg ccttcgggct tatctcagca
     3841 ttgttctgct ccacgccagc gatggcggat atgaaccgca ccacgaaagg tgcattgcta
     3901 ggcgcaggcg ttggtttact tactggcaat ggtgttaatg gcgtactgaa aggtgcagct
     3961 gttggcgctg gtgttagtgc agtaacagaa aaaggccgcg acggtaaaaa tgcgcgtaaa
     4021 ggtgcaaaag tgggtgccgc cgttggtgct gtcactggcg ttctgaccgg caatggcctc
     4081 gaaggggcga ttaaaggtgc cgtgattggc ggtacgggtg gtgcaattct gggtaaaatg
     4141 aaataaggaa tgatgatgaa aaaatttgct ttactggcgg gcctgtttgt tttcgcacca
     4201 atgacctggg cgcaggacta caatattaaa aacggtttgc cgtcagaaac ctatattact
     4261 tgcgcagaag ccaacgaaat ggcaaaaact gacagcgcac aggttgcaga aattgttgcg
     4321 gtaatgggta atgccagcgt tgccagccgt gatttaaaaa ttgagcaatc tccggaactg
     4381 agcgccaaag tggtagaaaa actgaatcag gtttgcgcta aagacccgca aatgctgttg
     4441 atcacggcaa ttgatgacac catgcgtgct atcggcaaaa aataatattt catatgtacc
     4501 gcgtcgatga atacgacgcg gttttttagc taacggcggg atcagaactt atatttcagg
     4561 cccaccatca ctgcggtgtc attgtagcca ttatcaccca gctgcacgcc cacattcccc
     4621 cagacgctgg ccgctggatt caggttgcca tttacgccaa gtttaatttc ccccaaatta
     4681 ctcaccccat cttgttcgac tttcacaccg ttcattgaga cagcaaaatc tttactgtta
     4741 tggatccagt tcatttcaac aaaaggttca aagtcgtcac cgtgcggacc gctgtttttc
     4801 tcctgagtac gaataaaggt gcggaagccg agtcgggtct ggatgttgtt atttgcgtca
     4861 ttctcaatac gtgagccatt ggcttccgtg tgcgcattat gatcgacgcc catccagata
     4921 gcctgtgcct gtggctggaa gatccacgta tatttcgccg cttccagacc aaaggattta
     4981 ttcagggcaa aaccgtaacc cgcttccagc gagacggttg cacctttcgc gctataggat
     5041 tcataggcta attcatcccc tttcacggag tttttaaacc agctatattg cgcccaggag
     5101 tcaatgtatg cgcctttttt actgatgtca tcggcaaacc aagtggcata cagccctgcg
     5161 ctatagcctc tgacactccc tttcgaacga taatccgaca cgctggaatg agttaagttg
     5221 tagtcgcggg cataaccagc catcactccc aaacgccaac tgtcactatc ggtaaaacca
     5281 ccggttaaca gatcgccccc taactgcgag acgtagcgat gcgaggtcgt tctcaactgt
     5341 ccgttgctgt cacgccaggc attatgcccg ccaatttgac gtagccaaag tcggcttgaa
     5401 cgctcctgtt cagttacagg atcgatgtag cgcgtttcac ccgcacggtc gttcagacgc
     5461 ataacaaaaa gagtgttagc ggctgccagg ttggcgacgt aacttccagc ttcggggcgc
     5521 aggacggggg taggcgcaac agtaggcgct gaacttcctc cggagtttgt ttcaccgccg
     5581 ctcgttgcct ggaagttagt cagataccag tttttattgg tcgcttcggt attacctcgg
     5641 gttaatgaat attcgtaggc accggcgaaa atcctcgaat ccttaataaa ttccccattt
     5701 gattccccct caacgctgat aatctctatc ccatttaacg tgtaagctcc gctgcctcct
     5761 gcattggtaa ctttaactcg ggttgttcca gaggtatttc cattaataac gactttatcc
     5821 gtagctgaat tatcatcatc caattcagtt ctgagatgca atacgccatt attaccaacg
     5881 taattttcag ttattgttaa tcttgttggt tcgacatccc gtccatctgc ccgggaaata
     5941 tagaccgtac tattatcaac cgtgaggttg ctcaccgttg agtcagcttt aatatcccag
     6001 gtactattat ctgaaagcga cagataggta tgagtgttat catctgttga aatattagct
     6061 gaacccgtta tttttgaatt agttgcatcg acggtcaaat gacctttaat attattgata
     6121 aaaacatctg cagccgatga tacctcacta tccgtaatgg tcacgttact cgtctcatct
     6181 ttatggtaga ggatttcatt tgctgaccgt agtgagctac cagataaaat atctaccgtt
     6241 gcttcctgac catttatgga agactccagc acaattccag acccctgatg cgcgactgca
     6301 gatgtatttt tcagcgtcac cgtgccgcca ttataaattt tagcaatcgc ggcgtcagga
     6361 cccgttgtat aaatttctcc ctggttaaat gaaactgtcc cctggcgggc agcgatggca
     6421 tgagcattgt ccccatgcgt ggtgatatca acatcggtta atgtaacgct ggcattggta
     6481 taatcaagcg tgtcaccagc cttgacgaca gtgccattag cgtacgcggc ataactttct
     6541 gttccgtggg ttgtgatctc cccttttgcc atatccagtt ttgctatggc attattttca
     6601 ttacttcctg tatcggcagt actttcttca tcaagagaag atacacacgc acacgcgctt
     6661 ccgggagatg cacacgcaag aacgccgact gcgtgattac ccgcagtaac aataaatgta
     6721 tcatctccgt cagaaaaaga gggggtaacc gaaccgtcag agttgactct tattatggtg
     6781 tcagtattca ggtttccact cgtttgactt agcaaaccaa ttgagtaatc ccccgttgtg
     6841 acaatcgtgt ttttaccctg caaaataaca gcaccgccaa attcacttac gattgcgcta
     6901 gctgttaatg ctgggcttag aggactattg tcagaaggat ataaaccgcc cgttgttaca
     6961 atgacgccat tgttaatggt taacttacta ccctctcctg caacggatac ccgtaacttc
     7021 cattacccgt tgttataact agcgcattat caatatcttt tacttttcca tcttttattg
     7081 ccacagcatg tttatattca tcgtgctgaa taaactgttc agcatatgca ttacccaccg
     7141 ccagagcaat aagtcccaaa ctaaacccgg ggagtttttt cagtttcata gattaactat
     7201 ccattcattg gattaacaaa cattcctggt caaataacct atgagctcag attaccaaaa
     7261 cggagggtaa agacaaaaac aaaagcacaa aaaatttaaa tagataaaca gcgacaatat
     7321 ataaatgata aataagtcgg cattatcatt tttcacctta ttttcatgac attgatcact
     7381 ttggggtgac tattttgttt aacaaataac tcttcgacat caaaattatt aatagttgcc
     7441 atggtaatta tttcggtgat atataaaaac aactgtgata acaccaaggt tgacaactat
     7501 ttgcgcaata attcctttcc ttcaatgatc caggcgttca ctgccagcaa cccattcagc
     7561 cgcatactga cagtgagaaa ctgaaagatt attgctgatc cctgtggcta acgagtcttt
     7621 ctcttaaatc gattaaccag attgatcgcc agccaaacaa gggcattaag caaatttatc
     7681 gtcacaagca cttctcccag cccggtactg ggttttgcaa taaggatgac caggagggtg
     7741 atcagcataa atgcaatgga aaagatgata tagagagtga aataatccag attattacta
     7801 ttattcatgc tactaatggc aatcctgtca aaactttcaa acaattatag caaacaaaat
     7861 aatgaacaat agtaacaaga ggcaacgagc taattagctg tctataatta caccgcacta
     7921 tggccttact gatcaatttt cactcaacca caaccttcac cttcggttta gaaaggataa
     7981 ttttgaccag gtcgatgtat gtaacaggtt tatagaaata ataaccctgc tgaaatgtga
     8041 tattattttg gttcagatag ccaagttgtt ctttcgtttc gacaccttca gcgacgatac
     8101 tgatcgaaag tttacgcgcc agatccaata cacaatccag aattcgggtt gattctgggt
     8161 ctgcgtttac gcggccaacg aaactgtgat caattttgat ataatcaata tgcaggtcat
     8221 gaagataaga aagccctgag tagccagtac cgaaatcatc cagcgcgatg acaaaaccat
     8281 tttcatgcag aatgttcaac cgctgaacca gactttcatc aacattcaat ggctcacgct
     8341 cggtgacttc cagaacaagg tttaaatcgc ggcgggtaaa actgtcacgg aaatttaaac
     8401 actcgtcgac aaacgtcggc gaaataatat gcgaagcgct aaaattaatt ccaatatgaa
     8461 aaccttccgg cagtttactc gcgatagcgt tcatctgtct ggcaacctga ttcatcaggc
     8521 tttgtgtaag cggaacgatt aatccggatt tttcagcaag tggaataaat gccgcgggtg
     8581 aaatatatcc accgtgaggt tgtttccagc gggctaacac ctcaactccc cgcaatgtcc
     8641 cttcccgacc atttaccaca ggttggtaaa aagggacgat ctccccatta tttattgccc
     8701 gtcgcaggat ctcttcaggc gttgcgcttt tattgaaata tctatcaagc aggaaggcag
     8761 cggcgctggc gattaacaga ataaatatta aaatcccaaa gcctttatcg ataagtctgt
     8821 agaagctaaa gagtggcggc gggttaaaga taatagtgaa atgatatttt gtggagtgaa
     8881 ccttctcggt atagggataa tgtccattaa gcgtcatcac atcagccgtt ggtccaataa
     8941 tgtcatccgc cacgcgtaat acatacctta cccctttcaa gggtacatta agcgccccgc
     9001 gaatatgttg atcacttatc gtaaccaaaa tgcgcgtatc tgcaaattgg ctctgataga
     9061 gcaggatagg taatctatta acggtgtcga ttgccggagc caacagtaaa ttactgtccg
     9121 ggacaacagg aatacgagac agcaggaccc gattcccagg cagggatgta caccagacta
     9181 ttccctgttt gagaatgatg attgtgcgca ggtgaggttt cagagctgct tcagtgccaa
     9241 gctgatattg ctcctcgagg tcgcattcct tcccggcaat gttaaccgct gtttgagtcg
     9301 cctgtgacgc ttcttcaagt atatgattga tattgttggc ggcatatctt gctcccgcca
     9361 ggtactctgc ttttgcggaa taccagagct ggatgttcaa aatagcgaca ccggtaataa
     9421 acaagcatat cgcaacgagg atgggtatga gtgtattgcg catttgcttg tcccggccaa
     9481 agggtttaat ac
//
LOCUS       DADLTY010000160         9407 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.161, whole genome shotgun sequence.
ACCESSION   DADLTY010000160 DADLTY010000000
VERSION     DADLTY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.161"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..890)
                     /locus_tag="JN334_003341"
     CDS             complement(<1..890)
                     /locus_tag="JN334_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7439379.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="JN334_003342"
     CDS             1131..1931
                     /locus_tag="JN334_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY7439380.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="JN334_003343"
     CDS             2009..2779
                     /locus_tag="JN334_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7439381.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="JN334_003344"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="JN334_003344"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HAY7439382.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="JN334_003345"
     CDS             complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="JN334_003345"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAY7439383.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5034..5756
                     /locus_tag="JN334_003346"
     CDS             5034..5756
                     /locus_tag="JN334_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7439384.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="JN334_003347"
     CDS             complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="JN334_003347"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAY7439385.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6285..7016
                     /gene="dnaQ"
                     /locus_tag="JN334_003348"
     CDS             6285..7016
                     /gene="dnaQ"
                     /locus_tag="JN334_003348"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAY7439386.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7149..7225
                     /locus_tag="JN334_003349"
     tRNA            7149..7225
                     /locus_tag="JN334_003349"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7183..7185,aa:Asp,seq:gtc)
     gene            complement(7356..8402)
                     /locus_tag="JN334_003350"
     CDS             complement(7356..8402)
                     /locus_tag="JN334_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HAY7439387.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="JN334_003351"
                     /pseudo
     CDS             complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="JN334_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8972..>9407)
                     /locus_tag="JN334_003352"
     CDS             complement(8972..>9407)
                     /locus_tag="JN334_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HAY7439388.1"
                     /translation="QPPMAEREMAWRLIRQLSFNYLPLADLDHRTGGQALRDLLNLFI
                     PAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVSCELTVDEEGFSGISPYLFG
                     LVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQRGSV"
BASE COUNT         2228 a         2414 c         2387 g         2378 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt
     4321 ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc
     4381 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
     4441 tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta
     4501 tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat
     4561 tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg
     4621 tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt
     4681 gtggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc
     4741 tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt
     4801 tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc
     4861 tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg
     4921 acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg
     4981 ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac
     5041 cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag
     5101 agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt
     5161 ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt
     5221 ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc
     5281 atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt
     5341 gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc
     5401 tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat
     5461 tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc
     5521 tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca
     5581 aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg
     5641 ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc
     5701 cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt
     5761 cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat
     5821 cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat
     5881 gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt
     5941 ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga
     6001 catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga
     6061 caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg
     6121 ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca
     6181 gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa
     6241 ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta
     6301 cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg
     6361 aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca
     6421 ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac
     6481 atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt
     6541 ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct
     6601 ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta
     6661 aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg
     6721 atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac
     6781 tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg
     6841 cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg
     6901 tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg
     6961 aagcccgtct cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac
     7021 atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat
     7081 gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa
     7141 cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg
     7201 ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc
     7261 tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa
     7321 aaatggccag acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc
     7381 agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct
     7441 gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg
     7501 atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg
     7561 gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc
     7621 tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc
     7681 tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag
     7741 aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc
     7801 tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt
     7861 tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc
     7921 tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata
     7981 tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca
     8041 gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac
     8101 gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc
     8161 tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga
     8221 cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg
     8281 ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg
     8341 tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc
     8401 atttaattat cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg
     8461 caccagatgg gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc
     8521 ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt
     8581 atccagaccg cccccagcgg actgggcgct gcgccatgcc tagtaaaact gcgtcagcca
     8641 gcggtgatga aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc
     8701 agttcggtgt agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga
     8761 ctgatgtgca ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc
     8821 agcgcgggcg tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc
     8881 ggaacggtac acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag
     8941 gggggggcct gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt cccgccctga
     9001 caggccaggt cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat
     9061 taatggaaac atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga
     9121 tgccggaaaa accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca
     9181 gtagtccgga accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc
     9241 gcacctggcg cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca
     9301 acgcctggcc gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact
     9361 ggcggatcaa ccgccatgcc atctcacgtt cagccatcgg gggctgc
//
LOCUS       DADLTY010000161         9249 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.162, whole genome shotgun sequence.
ACCESSION   DADLTY010000161 DADLTY010000000
VERSION     DADLTY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9249
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.162"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="JN334_003353"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="JN334_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HAY7439389.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="JN334_003354"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="JN334_003354"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAY7439390.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="JN334_003355"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="JN334_003355"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAY7439391.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2436..3296
                     /gene="pdxY"
                     /locus_tag="JN334_003356"
     CDS             2436..3296
                     /gene="pdxY"
                     /locus_tag="JN334_003356"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HAY7439392.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNNV
                     EEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="JN334_003357"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="JN334_003357"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAY7439393.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="JN334_003358"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="JN334_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HAY7439394.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6164..6799)
                     /gene="nth"
                     /locus_tag="JN334_003359"
     CDS             complement(6164..6799)
                     /gene="nth"
                     /locus_tag="JN334_003359"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAY7439395.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6799..7494)
                     /locus_tag="JN334_003360"
     CDS             complement(6799..7494)
                     /locus_tag="JN334_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAY7439396.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="JN334_003361"
     CDS             complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="JN334_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HAY7439397.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="JN334_003362"
     CDS             complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="JN334_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HAY7439398.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2381 a         2314 c         2345 g         2209 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat
     5581 taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga
     5641 ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc
     5701 tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa
     5761 tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttgg
     5821 cggcgatatg ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat
     5881 agccagtgta atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa
     5941 cctgattctt gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata
     6001 gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta
     6061 cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat
     6121 aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct
     6181 ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc
     6241 aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa
     6301 ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac
     6361 gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt
     6421 ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat
     6481 cttccggaac ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt
     6541 ctgctttgct gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccta
     6601 gttcaagcat cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa
     6661 cactgatatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac
     6721 tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca
     6781 gacgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc
     6841 agctgcttca gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat
     6901 cagtcccaag ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt
     6961 gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc
     7021 aaacaacgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt
     7081 tgcgcccgta ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc
     7141 gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc
     7201 taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac
     7261 cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag
     7321 cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag
     7381 agcgttagtg gcagtggatg tgaccgccaa tagcggacaa aggccgagca gttggaccag
     7441 tgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta
     7501 ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc
     7561 gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa
     7621 atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc
     7681 cgcaaaatgg gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt
     7741 ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag
     7801 cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg
     7861 tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt
     7921 taccagatag caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc
     7981 aaataacgtc ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt
     8041 gatggccgca gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt
     8101 tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta
     8161 gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg
     8221 atagccgccg aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag
     8281 ccgaccacga ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag
     8341 catagtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa
     8401 ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg
     8461 aatcgctttc tgccatagca accacacgcc accagccagc caggcgagat ttacccattg
     8521 ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga
     8581 atgacctgcg cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc
     8641 aatacctaag cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac
     8701 ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct
     8761 ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa
     8821 cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc
     8881 cagtacgacc atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt
     8941 cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac
     9001 gagagcttcg gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca
     9061 accaaaaaac cgcagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat
     9121 aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat
     9181 ttagtcctcg tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc
     9241 cgcggcaac
//
LOCUS       DADLTY010000162         9242 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.163, whole genome shotgun sequence.
ACCESSION   DADLTY010000162 DADLTY010000000
VERSION     DADLTY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.163"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="JN334_003363"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="JN334_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HAY7439399.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSLLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="JN334_003364"
     CDS             1828..3147
                     /locus_tag="JN334_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAY7439400.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3524..4669
                     /locus_tag="JN334_003365"
     CDS             3524..4669
                     /locus_tag="JN334_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7439401.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="JN334_003366"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="JN334_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="JN334_003367"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="JN334_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="JN334_003368"
     CDS             5815..6711
                     /locus_tag="JN334_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HAY7439402.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="JN334_003369"
     CDS             6708..7682
                     /locus_tag="JN334_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7439403.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="JN334_003370"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="JN334_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="JN334_003371"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="JN334_003371"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HAY7439404.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2259 a         2258 c         2317 g         2408 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cctcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatctca
     9241 ag
//
LOCUS       DADLTY010000163         9192 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.164, whole genome shotgun sequence.
ACCESSION   DADLTY010000163 DADLTY010000000
VERSION     DADLTY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.164"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..904)
                     /locus_tag="JN334_003372"
     CDS             complement(<1..904)
                     /locus_tag="JN334_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAY7439405.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNV"
     gene            964..1242
                     /locus_tag="JN334_003373"
     CDS             964..1242
                     /locus_tag="JN334_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439406.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="JN334_003374"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="JN334_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HAY7439407.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="JN334_003375"
     CDS             complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="JN334_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HAY7439408.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3396..3728
                     /gene="hdeA"
                     /locus_tag="JN334_003376"
     CDS             3396..3728
                     /gene="hdeA"
                     /locus_tag="JN334_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HAY7439409.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3844..4170
                     /gene="hdeB"
                     /locus_tag="JN334_003377"
     CDS             3844..4170
                     /gene="hdeB"
                     /locus_tag="JN334_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HAY7439410.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4234..4880
                     /locus_tag="JN334_003378"
                     /pseudo
     CDS             4234..4880
                     /locus_tag="JN334_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4922..5452)
                     /locus_tag="JN334_003379"
     CDS             complement(4922..5452)
                     /locus_tag="JN334_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7439411.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5608..6174)
                     /gene="slp"
                     /locus_tag="JN334_003380"
     CDS             complement(5608..6174)
                     /gene="slp"
                     /locus_tag="JN334_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HAY7439412.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6421..7644)
                     /locus_tag="JN334_003381"
     CDS             complement(6421..7644)
                     /locus_tag="JN334_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439413.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8225..>9192)
                     /locus_tag="JN334_003382"
     CDS             complement(8225..>9192)
                     /locus_tag="JN334_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY7439414.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2681 a         1827 c         1923 g         2761 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc
     3061 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
     3121 tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta
     3181 tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg
     3241 cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt
     3301 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
     3361 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
     3421 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
     3481 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
     3541 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
     3601 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
     3661 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
     3721 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
     3781 tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac
     3841 aaaatgaata tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg
     3901 tcactggtga acgcacaatc tgcgttggca gccaatgaat ctgctaaaga tatgacctgc
     3961 caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat
     4021 gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa
     4081 attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa
     4141 aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt
     4201 gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt
     4261 atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga
     4321 gcagggttac gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg
     4381 aaatatggtt ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt
     4441 gctgctcagg tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac
     4501 caaaatattg taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg
     4561 ttattggcag cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc
     4621 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
     4681 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
     4741 ctgatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
     4801 ttcatgccac aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga
     4861 aaggcgtttc aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa
     4921 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
     4981 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct
     5041 ccagaatatc ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
     5101 gcgaaagtga cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
     5161 aatcaatgat agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt
     5221 taatattaaa gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga
     5281 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat
     5341 gcaacattac gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa
     5401 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
     5461 cctggtgcgg acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt
     5521 ataataataa tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg
     5581 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
     5641 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
     5701 atcccacagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
     5761 catattcact tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg
     5821 aatggtaccg aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct
     5881 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc
     5941 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
     6001 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
     6061 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
     6121 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
     6181 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
     6241 tgaggtgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag
     6301 ttcaattccg ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt
     6361 gttttaaaat tacataaaat ctatttttat tcaatgatta taaagtgcta ataatcaata
     6421 ttatggtatt atattctgat aatgatttga taagcgaatt cttttgccag atggtatttc
     6481 tatttccacc ccataattta tatctttttc attaggcaga tctctcttta aataaaaggg
     6541 gtatgaaatc ttttcgggtt gaagtgtctc gtaatgaaat tctatgaatt catttttaga
     6601 cattgttgcc catttttgaa ttttattgta tagagagggt gttgtaaact cttgaagttg
     6661 tttataacat atttttttga actcttgctt atattttaat atgtcatcaa atgaaagatt
     6721 cactaccttt gcctctaaga aatcaattat tctcgaagca atttccgctt tcgcgcgtaa
     6781 agagttgtca ttaatagtta acaatgtaga aaacaaacca ttgatttgtt tgtcaagatg
     6841 ttgttcatat ttatgtagtt tggggatggg attttccttg tgtgaaaaaa tagtgttaaa
     6901 taaatttgcg tcagtgaaat ttatattaag tagatcttca aaataaaatt ttgataaggt
     6961 taggactagc ttggacatat cttgccctgt catgttgaaa tcaataaagt cgcatccagc
     7021 aagggcttcc atcctatttt catttttact gtgctcaata tacagcttcg ccatttttaa
     7081 gtacaagctt tgaatgtctc ttatatcagt tggtaagtca gctcgttgcc cagcaaaatt
     7141 tttattatcg gaagaaacgg tttggacact gaatctttcg ggtattcctt cttctacgag
     7201 aattttagtt atgattggat tgttgggttc tggattaacg acaacaatga tttgacatac
     7261 agaatcctta tccttttcgt atctaatgtt atgaatctga ttaagtattg attgtgtctg
     7321 ttgcgtaagt ggtgtcgaac ttatcgtttc aatgttatta ataagactta caagttgaac
     7381 cacaactttt gagtagtctg catttgtata tgtggtgttc gtgtggcatg cattaatttg
     7441 ttggagttgt tgttgcgcta aatcaaattg ttcagggttg tatccttctc cagagaatgt
     7501 ggaattaatg ggttgttgtt cttcccaatt atcacaaggc cacttcggtg tgtctggcgt
     7561 tttaacatta tcggtatttg ttgtgcagtt ataaatatcg ccgtgatatg tatttattat
     7621 acctggggta aagtcaattg acataacatt ccttattcat catctgtttt atgctaatgt
     7681 ttttaaattg gataattaga acaggaatgg attttcttct acacacaata aatataatgc
     7741 gttatatctt cctgatataa ttacctttaa attccctttc atttccaccc ggtaatattt
     7801 gtgtataaaa aaagggcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaaa
     7861 cgctcatttt aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg
     7921 ttttagatgt gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga
     7981 ctgaaatctg ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga
     8041 taaaatgtca gagaagcatg attacgtggt tattgtctgt tgatgacttg ttgaccgagc
     8101 cgtcatcatt ctcaatgcat tagttaaatc atttgtgatt tttaaggatt atcctgtcta
     8161 tgattttcta cttagaagga tatggcacta actatgcatc ttatagttta ctgctggcat
     8221 ctatttaaaa tagcaattga gtcaaatagc ccatcaagat agccatacct aatataaccg
     8281 tcaggaatgc agctatcatc ggcattctga acattgattt aagtaatatc acttccgtca
     8341 ggcttgcgcc ggcactgccg atgattaatg ccattaatgc tcccagaccc attccttttg
     8401 tcatcagaac agatgccaga gggataaccg cctcagcccg gatatacagc ggaataccga
     8461 caacggcgct taatgggatg gcgaagggat tatctgctcc tgcatgagcg gcaatccacg
     8521 ctgagggaat aaagccataa ataaaagatc ctattaatac tcctaacaaa agatatggca
     8581 gtacatcttt aaactgttgc aacgcatctt tggtggctag ttttatggga ttaatactta
     8641 ctacagcttt ggtactgcaa caggtaacta cggggttctc aatggcttta gccgctggac
     8701 tacaacaact cacttttatg ggcgtatatg tcgttcccgg cgaagttttt gctggagcac
     8761 aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaacccc agagaatcca
     8821 ggataatact ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac aaggtgactt
     8881 tccaaccaaa ggtcatccac attagtccga cgataatggg attaagtaat ggtgaaacaa
     8941 ataaaaaagt gagagtcgga ccaaacccgg ctttcgctga caacaatcca cgtaacatgg
     9001 gtattgtcga gcaactacag aacggggtaa cggctcccaa cagagcagcc aggagatagc
     9061 ctctcccttt tctggccccc atcatctgct ggattttatg gtctggcacc ttttgtctta
     9121 tcaggctgac accggcacta atcactataa acagcaacga aagctcgacg gcgagaaaca
     9181 caaacatctc tg
//
LOCUS       DADLTY010000164         9050 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.165, whole genome shotgun sequence.
ACCESSION   DADLTY010000164 DADLTY010000000
VERSION     DADLTY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.165"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            1..3051
                     /locus_tag="JN334_003383"
                     /pseudo
     CDS             1..3051
                     /locus_tag="JN334_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3061..6707
                     /locus_tag="JN334_003384"
                     /pseudo
     CDS             3061..6707
                     /locus_tag="JN334_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6768..7175
                     /locus_tag="JN334_003385"
     CDS             6768..7175
                     /locus_tag="JN334_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439415.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7717..8805
                     /locus_tag="JN334_003386"
     CDS             7717..8805
                     /locus_tag="JN334_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAY7439416.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8816..>9050
                     /locus_tag="JN334_003387"
     CDS             8816..>9050
                     /locus_tag="JN334_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439417.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFA"
BASE COUNT         2349 a         2339 c         2198 g         2164 t
ORIGIN      
        1 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
       61 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      121 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      181 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      241 atctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      301 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      361 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      421 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
      481 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
      541 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agcactggcg gaacttctag
      601 tgcaaaaaga agagaatagt tggcgcatta tgctaatgac gatgtttatc tcgcaaccag
      661 cactggcaga acaggtcatt ccctggcttt cgacacccgc agttgcagtg ctgaaatcat
      721 gccagcacca actgacacag ccctcaaacc atgccagcgc cgatctactg ccagccatag
      781 tagtctcccc accatggatt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag
      841 cgccattagg catcgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg
      901 cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt actaccaacc
      961 tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa gctcccgaag
     1021 cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa tttaaggtgt
     1081 ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg ccgctggagg
     1141 ctggagaaag cagtaaaagc atggaatgcc cttactctat ctccacatac cgataccgaa
     1201 tacgccatgt tacattttgg actcaaaggt ttacctggtt tagttaactc aattacacgc
     1261 tatccacagg aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc
     1321 gtcgcccgtg ccttcaacaa actcaaaacg ctacgcgaaa acgcccgcac ctggctgttg
     1381 aaatacccgg aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa
     1441 gcacaggata acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta
     1501 ctgcaagaaa tcgcccggcg ttataaccag tcggaagtaa cagacgcggt gaacgctctg
     1561 cttgcgctcg atcccttaga taatcacccg acaaaaatcc ccactcttcc gaccttttat
     1621 cagccatcga tctggacgcg cccggtatta aaagcaaacg cccaatcact gccagataac
     1681 gccctcctcc acctcggcga aatgctccgc ttccctcagg aagaggctct gtatccggga
     1741 ttattgcagg tgaaagacgc ctgtaccgct gactctctgg ctgaatttgc ctgggatctg
     1801 tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta
     1861 ggcgttctcg gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct
     1921 ggtgaatccc agcataaacg cgccaccgtt gggttggata ttctcgccgc tatcggtagt
     1981 gatatcgccc tgatgcagct taacggcatt gctcagaaac tgaaattcaa agcattacag
     2041 gagcgggcaa aagaaaagat tgccgacatt gccgagagcc gcgaactcac ggtagcggag
     2101 cttgaagatc ggttagcacc ggatctcggt ctggatgata acggttcgtt gctgctggat
     2161 tttggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat
     2221 gtttccggca gccgcctgaa agacctgcca aaaccaaaca aaagcgatta tgagtcacag
     2281 gcgaacgatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac cgtcgcggcc
     2341 cagcaggtag caaggcttga atccgccatg tgcctgcgtc gccgctggtc gccggaaaac
     2401 ttccaactct tcctggttgt gcatccgctg gttcgccact taacccgccg tctgatttgg
     2461 ggcgtttata gcgccgacaa ccagctactg gcctgtttcc gggtagcgga agataacagc
     2521 tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcatt
     2581 cctcacgttc tggaaatatc accgacggat gctgtcgcct ttggtcagct ttttgccgac
     2641 tacgaactgc taccaccgtt ccgtcagctt gaccgtaaca gttacgccct gacagaagcc
     2701 gagcgcaacg ccagcgaact gacccgctgg gcaggcagaa aatgcccgag cggccgggtc
     2761 atggggctgg caaataaagg ctggataaag ggcgaaccac aggatggagg ctggatgatc
     2821 aaacctctcg gtcgctggtc gttaatcatg gaaatcgacg aaggttttgc ggtgggtata
     2881 tcaccagccg aacttagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa
     2941 gcggaaagct atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat
     3001 gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc
     3061 atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa
     3121 ggcaaaacac cactcagtca tcgcaattgg ccgggcgaac cagtgcctgt tatcactgaa
     3181 agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaaacatt
     3241 gtctggcgtt atgaaaattt ttcaccggag tggcagtccg ccattacgca ggccatcaac
     3301 ttgatcggcg aacacaaacc ctcagtcccg gcccgcacaa tggcggcact agcctttatc
     3361 gcgcaaaatg acaaccaaca gttgctcgac gaaatcgtcc aacaagaggg gctggaatat
     3421 gcgactgagg tggtgattgc acgccagttt attgcacggt gttatgagag tgatcctctg
     3481 gtagtcacat tgcagtatca gaacgaggac tatggctatg gttatcgctc agaaacctat
     3541 aacgaattcg atctccgact gcgtaaacac ctctctctgg cagaggaaag ctgctggcag
     3601 cgttgcgccg acaaactcat tgccgcactg ccaggaataa ccaaagttcg ccgccctttt
     3661 attgcgctga tcctccccga aaaaccagaa atcgccaatg agttggtaag ccttgaatgc
     3721 ccgcggactc attttcattc taaggagtgg ttaaaagttg ttgctactga ccccaaagcg
     3781 gtgagaaaac tcgaacgcta ctggagccag gatatattta gcgatcgaga agccagctac
     3841 atgtcgcatg aaaaccactt cggctacgcg gcctgcgctg cccttttgcg cgaacaagga
     3901 ctggcagtcg ttccgcgcct cgcgatatat gcccataaag aagattgcgg cagtctgctg
     3961 gtacaaatta accatccgca agtcatccgc accttgctgt tagttgctga taaaaacaaa
     4021 cccagcctgc aacgtgtggc taaatacagt aaaaacttcc cccatgcgac actcgccgca
     4081 ctggcagaac tgctggcgtt aaaagaacca ccagcccgcc caggttatcc aatcatcgaa
     4141 gacaaaaagc tgcctgcaca gcaaaaagct cgagatgaat actggcgtac gctgttacaa
     4201 acgctgatgg catcgcagcc acaactggca gcagaagtga tgccgtggtt aagtactcaa
     4261 gcccgggcag tgctgaatag ttatttatcg gcaccgccca aaccagttat tgatagcacc
     4321 gataacagcc aaatgcctga aatcctcgtt tcaccaccat ggcgtgggag aaagaaaaag
     4381 acttccatac gtcacttttc gctgcctgat cttacgcttg caccgcaaac gtacaggtcg
     4441 ccgaataaac actggacgcc ggaacagcaa actgcaattg accattttag cgccatgtct
     4501 tttgacgaac gtcttgctac gagaggagcg gatatcttct tgcgggaatt aggttttgac
     4561 gttcatagcc tggaatttaa taagcgtctt cttaatggac agttggatgc ctttgatgct
     4621 cagcaactgc aagaatacac atcaatagga ctattgcgta cttatcacgc gcatcaggcc
     4681 aaatctaaac agtaccggga agacgccacc aatgcattac tggcacaaga tagcattgcg
     4741 ttgatgggag ggtggcgaac ttttcagcac ggattttact atggtaatga acagcgttgg
     4801 gggatttgga atctgtacct tatcgcgcag atgccgcgcg aaatggcggt atcgtgctgg
     4861 caacaaattg tcgaggctga ttttcattac accggggtgg aatatttgtt aagtgtgctg
     4921 ggtaccgatg ctttgcctgg attaaacacc gcctttgccc gacatccaaa agagatattt
     4981 ccgctgctga ttcatttcgg cgcaacagaa ctggccctgc ccattgcccg cgtctggcac
     5041 ctttttgcgg gccagcgtaa tctggctcgc cagtggattt tacaatggcc ggaacatacg
     5101 gctactgcac ttatccctct tgtcttcacc aaatctagcg ataaaagcga agctgcatta
     5161 cttgccctgc gtttacttta cgaacatggg catggcgaat ggctacaaac tgtagccaat
     5221 cgttggcagc gtacagatct atggcctgcc ctggaacaat tacttaaaca gagtccaata
     5281 gagatttacc cggcacgcat tccaaaagcc cctgatttct ggcatccgca aatgtggtcc
     5341 aggccgcgcc ttatcactaa taatcaacct gttaccgatg acgctctgga aattatcggc
     5401 gaaatgctgc gctttaccca ggggggacgt ttttatagcg ggctggaaca actgaaaacg
     5461 ttctgccagc cacaaacgct ggcagctttt gcctgggatc tcttcactgc gtggcaacaa
     5521 gctggtgccc ccgcaaaaga caactgggca tttctggcgt taagtctctt tggtgacgaa
     5581 agcacggcac gggatctaac gacacatatc ctcgcctggc cacaagaagg caaatctgcc
     5641 cgtgcggtca gtggcatgaa catccttacc ctgatgaata atgatatggc gctgatgcag
     5701 ctgcatcata tatcgcaacg ggcgaaatcc cgccccttac gtgataacgc ggcggaattt
     5761 cttcaggtag tcgcagaaaa tcgcgggcta agccaggaag agttagcgga cagattagtc
     5821 ccaacactgg gccttaatga tccgcaggcg ttgagttttg attttggtcc ccggcagttt
     5881 accgttcgct tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa cgttcgccag
     5941 aaaagtgttc cccggttgcg cgccgatgac gatcaactga aagcgcccga ggcactggcc
     6001 cgactaaaag ggttaaaaaa agatgctact caggtgagca aaaacctgct cccgcgtctt
     6061 gaagctgccc tacgtaccac ccgacgctgg tcgctggcag attttcattc tctgtttgtt
     6121 aatcatccct ttacccgtct ggttacccag cgattaatat ggggggttat ccggcaaatg
     6181 aaccgcgtcg tttactcaac gcctttcgtg tggccgcaga gggggagttc tgcaatgcgc
     6241 aagatgagcc aattgacctg cctgcggacg ctctgattgg cattgcccac ccgttagaaa
     6301 tgacagcaga aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc
     6361 cttttcgcca gttgtcgcgc cgcacggtgc tgctcacacc tgacgagtca accagtaaca
     6421 gcctgactcg ctgggaaggt aaatccgcta ccgttgggca acttatggga atgcgataca
     6481 aaggctggga gtcaggttat gaggacgcat ttgtctatga cctgggtgag taccggctgg
     6541 tccttaagtt ttcacccggt tttaaccact acaatgttga tagcaaagcg ctaatgagct
     6601 tccgttctct tcgagtgtac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt
     6661 ttgatttgag tgaggcgtta agcgcacctg acgtcatttt ccattaaaac aaagcgggca
     6721 gtgatgcgac tgcccgttat ctacacgact taccagcggg gaaagcgatg atcgtgcaaa
     6781 aagagctggt tgctatttac gattatgagg tccctgtacc tgaagatccg ttttccttca
     6841 gacttgagat ccataaatgc tctgaattat ttacaggttc cgtctaccga ctggaacggt
     6901 tccggctacg tccaacattt catcaacgtg atcgagagga tgttgacccg ctaataaatg
     6961 atgcgttgat ttatataaga gatgagttta ttgatgagca aaaattacga ggcgaatcac
     7021 ctgaacctgt aatagtaatt tttaattgta aactacagaa tatattcaac caacaaatag
     7081 aattatatac tctagataat tcaagtcgca gcacatgcaa cttgaagtat gacgagtata
     7141 gctttattaa aggggggaac ggtattcccc cttagatgcg tttcaatttg cccgttactt
     7201 caacaaatca atcaaccgta acagagccac taatagctcc agtacagcaa tgagcacggt
     7261 taacttttgt gtcagcgtgt ccatccgctc tccttattaa ggagcacaac accagcacct
     7321 tacccttaca ctgcctgtcg atactggttt acgtttgttg agtcgccatg ttgcactccg
     7381 gatcagagca ctacctgcgg caccacccgt ccggtagcca ggacttgaaa taggttcgat
     7441 ccggcgcaac ttcccaacag tcaggattat aaatctcatt atcttaattg caacactgtc
     7501 cagaggtggt tataattcgt tcgttggaac acgatgttgc acaggcagtg gtgttaggcc
     7561 tgaaaatagt aaataaaaga acctgcctca ccagcagttt tttttattta ctgtgatctg
     7621 ctttccagat atttttcgct caaacaacta atgcgccaaa catttattgc gcgtaaaata
     7681 tcgtttattt cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg
     7741 cagcgtccgc ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat
     7801 aacgcacctt gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt
     7861 ggcgatagcg cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt
     7921 atgttagtca ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca
     7981 aaatctctcc tgtctgaatt actggcaacc tctattagcg gcgatgccgg gttaaccatc
     8041 cagggcggcg catctactac cgaagatcaa atcaagtacg gctggaacta cgccctgctt
     8101 atcaatcatg gtccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc
     8161 gacggcaaaa tcgtccgttt tgaagagata acgcgtacac cgctggaagt gcaagactgt
     8221 ctgttaggta tgctttctga cagggtgatg acggtgccgg aactcactgg tgaagccagt
     8281 cagctctatg cgcgagaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc
     8341 gtcaatgaaa tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt
     8401 atggatttcg cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat
     8461 agcggtattc cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc
     8521 gatctgcgtg ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc
     8581 accgctgaag ccgtgaacgt ggcccacgcc gtaggcgtcc gcgcctggtt tttagcgaat
     8641 cgcgcaggtg agccagccga cctggtggac tgcattgccg gaaccatcgt caaagataac
     8701 gaagaagatc gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac acataaagaa
     8761 gctcactggc aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag
     8821 cgagccgtta attgtcggca tccggcatca tagtccggcc tgcgcccggc tggtgaaatc
     8881 gttaatcgaa agccagcggc cacgatacgt gttgattgaa ggccctgctg attttaatga
     8941 ccgggtagac gaactgtttt tagcccacca gcttccggta gctatttaca gttattgcca
     9001 gtatcaggac ggtgcagccc ccgggcgtgg tgcctggacg ccatttgctg
//
LOCUS       DADLTY010000165         9029 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.166, whole genome shotgun sequence.
ACCESSION   DADLTY010000165 DADLTY010000000
VERSION     DADLTY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.166"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="JN334_003388"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="JN334_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HAY7439418.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="JN334_003389"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="JN334_003389"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAY7439419.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="JN334_003390"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="JN334_003390"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAY7439420.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="JN334_003391"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="JN334_003391"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAY7439421.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="JN334_003392"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="JN334_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAY7439422.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /locus_tag="JN334_003393"
     CDS             5562..6035
                     /locus_tag="JN334_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HAY7439423.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="JN334_003394"
     CDS             6064..6606
                     /locus_tag="JN334_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAY7439424.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="JN334_003395"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="JN334_003395"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HAY7439425.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="JN334_003396"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="JN334_003396"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAY7439426.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="JN334_003397"
     CDS             8003..8260
                     /locus_tag="JN334_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HAY7439427.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="JN334_003398"
     CDS             complement(8236..8790)
                     /locus_tag="JN334_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAY7439428.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2258 a         2192 c         2253 g         2326 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagag
//
LOCUS       DADLTY010000166         8996 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.167, whole genome shotgun sequence.
ACCESSION   DADLTY010000166 DADLTY010000000
VERSION     DADLTY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.167"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..923
                     /locus_tag="JN334_003399"
     CDS             <1..923
                     /locus_tag="JN334_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7439429.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="JN334_003400"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="JN334_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HAY7439430.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="JN334_003401"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="JN334_003401"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HAY7439431.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="JN334_003402"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="JN334_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HAY7439432.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="JN334_003403"
     CDS             4446..4805
                     /locus_tag="JN334_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HAY7439433.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="JN334_003404"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="JN334_003404"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HAY7439434.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="JN334_003405"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="JN334_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HAY7439435.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="JN334_003406"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="JN334_003406"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY7439436.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2044 a         2171 c         2434 g         2347 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt atttta
//
LOCUS       DADLTY010000167         8993 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.168, whole genome shotgun sequence.
ACCESSION   DADLTY010000167 DADLTY010000000
VERSION     DADLTY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.168"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="JN334_003407"
     CDS             complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="JN334_003407"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HAY7439437.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="JN334_003408"
     CDS             complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="JN334_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HAY7439438.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4606..5763
                     /gene="mdoC"
                     /locus_tag="JN334_003409"
     CDS             4606..5763
                     /gene="mdoC"
                     /locus_tag="JN334_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HAY7439439.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5771..7252)
                     /locus_tag="JN334_003410"
     CDS             complement(5771..7252)
                     /locus_tag="JN334_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HAY7439440.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="JN334_003411"
     CDS             complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="JN334_003411"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HAY7439441.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7822..8133)
                     /locus_tag="JN334_003412"
     CDS             complement(7822..8133)
                     /locus_tag="JN334_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7439442.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="JN334_003413"
     CDS             complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="JN334_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HAY7439443.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8645..>8993)
                     /gene="csgA"
                     /locus_tag="JN334_003414"
     CDS             complement(8645..>8993)
                     /gene="csgA"
                     /locus_tag="JN334_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HAY7439444.1"
                     /translation="NNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGAD
                     VGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVT
                     QVGFGNNATAHQY"
BASE COUNT         2252 a         2328 c         2131 g         2282 t
ORIGIN      
        1 aacgccggta tttttattgg ctcgttgttt ttgccggatg tggcgtgaac gccttatccg
       61 acctacaaaa tcttgcaaaa tcaataaatt gcaggaacga tgtaggcctg ataagcgtag
      121 cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga
      181 ttgagcttta tcccttcgta ataactcacc catgaagaat atctttcagg ggaattccag
      241 acgcggaaat gcagacgggc catcgtcacc ggatcgctta gcagcaccag gcgacgatcg
      301 cgattcagct tctctggcgt ctcgttaagc gcctgttcaa cgtggcggtc acgggcgatt
      361 tccagcacct tactggcgcg gtgacgcgct gtcgccattg cggttgccag agcgttaaat
      421 gacgggttaa acactgcgtg cataaagcca tcgtcaaggg agcgttggcg attcatctca
      481 aggaaccgat cggtatcaac cagcacctgc ggcggtgaat actcttccgg gatcaggaac
      541 agtttccagc gtttggtgcg cagaccaacg gtggcacggc tggaaatcac cgaaacaaac
      601 ggtgacagga tcaacgagaa gacaatcggt gccagccaga acaggaaacg cagatccagc
      661 cacgccatcc caacagccca cactaacccc aacagcagct gtgagccgtg gcgtttgaac
      721 gcttcgcccc atgaagtgga gtcatcatca cgctgcggtg aattccacac cacttcccag
      781 ccaaggaacg cgctgacaac gaagaccgta tggaacagca tgcgtaccgg agccagcagc
      841 acggaaaaca gcacttccag cagcaacgat aatgtaacgc gccagaagcc gccgtattct
      901 ttcgttcctt tgcaccagat aagcaaaatg ctcaataact tcggcaggaa caacagcacc
      961 atggtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca caccgggaac
     1021 aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catgcactac ctgcaatgca
     1081 gtagagagcg cgaggaacat aaaccacagc ggagcggaga gataagacat cacgcccgtc
     1141 aggaacaccg cacggtgaac cgggtgcata cccttaacca ggaacagacg gaagttcatc
     1201 aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt aggcggcaat
     1261 tcttcataag aacccgggag atcgtaagca atccagaccc cccaacctgc acggcgcatc
     1321 aacgccgctt ctacgaagtc atgtgacagg attgaaccgg caaaggaacc ttcgcccggc
     1381 agcggagcca gtgcgcagtg ctcgataaat ggtttcacgc ggataatcgc gttatgtccc
     1441 cagtagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac
     1501 acgcgggtcg cgaactgctg acagcgcgca tacagcgtat ccataccgga cgctttcggc
     1561 gacgactgaa tgatcccggc gttcgggttg gcttccatca agcgcaccag gccgcataaa
     1621 caatcaccgg tcattaccga gtcagcatcc agcaccacca tgtagctgta ctggctgccc
     1681 cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcggcg acggcggcgg
     1741 cgatagaaaa tctgaccttc gccaccgact tcagcgataa gctccatcca ggctttttgc
     1801 tctgcgacgc agatatccgg gttataactg tcactcagaa tgtagacatc aaagtgtttg
     1861 gcattcccgg tggcttttac tgattcccac gttgcacgca agccagcaaa aacacggttc
     1921 acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt taatggttca
     1981 tcgccaactg ttgacgcaga tatactgtat ttatcgcgac caataagcag ttgcaggaag
     2041 cccatcaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc aaaaaggatc
     2101 aggataccgg tttgcagcat ataaggcaga agctgcataa aggaaaccca caaatcctga
     2161 ccaaccatat ccataggatt aatcagcgcc caaccctgat aaggaagaat ggtcttcata
     2221 taccaggtcg cgacaacagt ttgcgcgagc gtcaggatca acagaatgta acggcggatg
     2281 gtaccaacgg tacgccactt ttgctcgctc tcctgctctt ctttggtcaa acgagccaga
     2341 tagcgcggcg tgacatcgcg tccacgcagg cgatcccaga aacggcctac cgggttggta
     2401 cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt aagctgatcg
     2461 cgcccttcgt cgtctttaat taactgtcca tcagcaagtg aatctggcca ggcttgttcc
     2521 agacgcgcct ttaccgagcc ttgcggggaa tcatcctccc gcgcccaagt gcggtgttcg
     2581 gcatccagcg cctgatgaac ggcgcggata tcagtcttcg gcaatgctgc tttctcgctt
     2641 gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag gtaactggta
     2701 gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcacgca tttcagtgat
     2761 tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg taaccgggtt
     2821 atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt gcgctgtgac
     2881 cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca caaaggcgat
     2941 agtaccgtca ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt
     3001 ttgttgcacc catgcgttat ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat
     3061 ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg tccagtaagc
     3121 gacgatgtta tcgttggttt catcgttggt tggaatttcc accagctcaa cgctgccttt
     3181 gccccactcc cctttcggag tcacccatgc gcttggacgg agatcgtaac gatcatcgag
     3241 atcttcaaag cgggagaaat cacgacctcg ttgcaacaga ccaaagcctc gcgggttttc
     3301 catggagaag ctgctgaccg ctaaatgttt cgggttattc aacggacgcc agatccattc
     3361 gccattacca gcatggatag acagaccgtt agagtcgtgc aactccggac gatagttatt
     3421 tgcaggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg caacccccag
     3481 tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg tgtcacgccc
     3541 tggcataacc acgaatttgt aagcacctgt cgcacgcggc gagtcaagca atgcataaat
     3601 ggttaaacgt ttatcagtcg gttttggacg ctcgatccag aactctttga agcgtggaaa
     3661 ttcttcaccc gatggcaaag cggtatcaat tgctaggccg cgggcagaaa ggccataaac
     3721 ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga tttcatcgtt
     3781 tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac
     3841 cgtgtctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt atttgattcg
     3901 tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt acataccctg
     3961 atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt tgtcatgatt
     4021 aaactggatc tgctgatagt ccgcgtattt catgtcgcgg aaaacggagg gcaagttgct
     4081 tttaggcgcc tcatagcctt tcccggctaa ggattgagct tgctttgcga catcatcaat
     4141 actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac tcaaccaacg
     4201 cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc tttgtgtgct
     4261 tatatcgatc cgatctattt taatggataa ccagccattc cgacaactga atcccggtat
     4321 cgttcagcac gctggctcat ggattaagca cctgattgca accgttttca ctttccgtgc
     4381 ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaaatt tatttatcaa
     4441 ccgtcttaca gacgcttcca ttctggttag tggtagcgat gatgctatgc ataaaaacct
     4501 tgaagcctgc gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata
     4561 acccgacagg gataagacga aaaatccgag attagttaac catatatgaa tccagtaccc
     4621 gcgcaacgtg aatatttcct cgactccatc cgcgcctggc tgatgttgtt agggatccct
     4681 tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc cgaaccgtca
     4741 ttgtggctaa ccctttttaa tgacttcatc cactcgttcc gcatgcaggt atttttcgtt
     4801 atatccggat acttttccta catgcttttt ttacgctatc ccttgaaaaa atggtggaaa
     4861 gtacgtgtcg aacgtgtagg tatcccgatg ttaacagcca tccccctact gacattaccg
     4921 caatttatta tgctgcaata tgtcaaaggg aaagcggaaa gttggcctgg gctatcattg
     4981 tatgacaaat ataatacgtt ggcctgggaa ttaatatcac acctgtggtt tttactggtg
     5041 ttagtagtca tgacgacgct gtgtgtatgg atatttaagc gcatcagaaa taatttagaa
     5101 aattctgata aaacgaataa aaaattctcg atggtaaaac tatcggtgat ttttttatgc
     5161 ctcggcatcg gctatgcggt aataagaaga acgattttta ttgtgtatcc acccattctg
     5221 agtaatggca tgttcaattt tattgtcatg caaacgctat tttatttacc gttctttatc
     5281 ctcggcgcac tggctttcat tttccctcat cttaaagcct tgtttaccac gccgtctcgt
     5341 ggctgtaccc tcgctgcagc attggcgttt gtcgcttatt tactcaacca gcgctatggc
     5401 agtggcgatg cctggatgta cgaaaccgag tcggtgatca ccatggtcct cggcctgtgg
     5461 atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc agcgcgggtg
     5521 acttattttg ttaacgcatc gctgtttatc tatctggttc accacccgtt aacgctgttt
     5581 ttcggcgcat acattacacc gcacatcacc tccaactggc ttggttttct ctgtggcctg
     5641 atattcgtag tagggattgc gataattctg tatgaaattc atttacgcat cccgttactg
     5701 aagtttttgt tctctggtaa accggttgtt aagcgtgaga acgataaagc accagcccgt
     5761 taagccacat ttacaataac cattccacgg gcaatatcga cgccagtctg accataaccc
     5821 gcttccagaa actggtggcg ggttcttttt tgagaataac ctctttcttt gcatgacgat
     5881 caacccagtt gatccgtccc cacctgtcca gacggagctg ccaggccgca tcgtactggc
     5941 tctgaataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc acgaagccca
     6001 tttcagtatt gagcaatgtt gaacgcggat cgaaattgaa agagccgata aacaccgttt
     6061 taccatcgat gctaaaagtt ttagcatgca ggctggcacc ggaattaccg gttatgccgc
     6121 gatcgtgtaa cgtactgctt tgttcacgcg tcggcttgag ttcatataat tccacgccat
     6181 agcggagcaa ttttttgcgc cagcgcgcgt atccggcatg gacgacagca acatcgttag
     6241 cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc cgtaagagtt
     6301 gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg cgttcactgg
     6361 gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt gccttgccct
     6421 cccctttcgc cggatcatca cttaataaac gtgtcttcgc ccagataagc ggcaacgttc
     6481 catcaaccag atgatttata aatggactgg attccatttt gcgtaaataa cgatgcgtca
     6541 tggcatcgtt atgccaggag gcgggtaact cgatgcgatc cgccatttca ccttccggga
     6601 catccagcac ctgctgtaag ggtgaaaccg atttgcaata ccagtagcgg gcgaaatcat
     6661 cggcaacatc ctctaccacg gggcctattg ccatgacatc taaatccgaa aaaagtggct
     6721 cttcccctgc accaaaatag gcatcaccaa tatttcgtcc tcccactagg gtaaccacgc
     6781 catcgacagt gaaactttta ttgtgcatcc ggcgattgag acgggaaaag tcggtgatat
     6841 atccaagcgg acgtagcaag cgaaacgaga aaggattaaa aagccggact tcaatgcgtg
     6901 gatgactgtc aagcaagcgc aaaatgtcgt caagtccggg cgtattgttg tcgtccagca
     6961 acaaacggac gcgaacgcca cgctttgctg cggctaacag ggcggaaaac agtaaccgcc
     7021 ccgacatatc gtcctgccag atgtaatact gaacatccag cgtatgctct gccatttcgg
     7081 ccagacgata ccgggcggca aacgcatcca gacttttctc cagagggaaa agaccacact
     7141 gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccggggc aaatcattca
     7201 tctccttgtt gggtaaggag tctttcgtag aggtgggcgt tttcttcatc ataacagaca
     7261 aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt tttaactgcg
     7321 atttcagccg ctgccgcacg agggtaacta taaaccccag tactgattgc aggaaaagcc
     7381 actgacgtat agctgtttgc cgccaccagt cgtaggctat tcagataggc atcctgcaaa
     7441 agctggtctt cgttttgttc cccaccacgc cagactggcc cgacggtgtg tactacagct
     7501 ttagcgggaa gatcgcctgc aagcgtaata acggcatgcc ccgtagggca atcgccctgc
     7561 tgttgcctga cttttaaaca ggcatccagc agggccggac ccgctgcgcg atgaatggcc
     7621 ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat cacatcaacg
     7681 gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac gcctccttac
     7741 accttcttgt ttcttaagtg tacagcaggc tttttggtta aatcgcattt ttctgctcag
     7801 caacgtgagt tgttcagaaa cttagttata ttcgatgttc attacagcca gctttttctg
     7861 tttatcaaga tactgcactt ttactgaggc tatttgcgcg tttttgacat tgccgctcat
     7921 taacgctttg ctggagtagg ttttgcccgg aatagaacca ttctgtgggc agttcatttc
     7981 tacgggtgat gactgggtgc tgacgtcaca tgccggttcc acaatttggc cataaaaatg
     8041 gatcacgccc ccttgcgcgg tgcttcctgc aatggcaata aaaggaaaaa acaaactgcc
     8101 gagcataaga gagtctaaaa aaggtctgaa catttattct atttcctcaa tgattagtca
     8161 tccttgaagg ttgtgttatc catactttca ggttggctga ttcgcagcag accattctct
     8221 ccagattcat cttatgctcg atatttcaac aaattaagac ttttctgaag agggcgtcca
     8281 ttgttgtgat aaatgaagtg cctgtccatc agaaacagta acaactattt tcacccgatc
     8341 ttccggggaa atatttaaac tcaacttcgt caaagcaatg ggttgattag caggcaatga
     8401 gagggtcttt tcttgcttcg tctgactttg ccctgaactg ccttcgcgca gggacaatat
     8461 ttgtactctg cacagacaag attgagtaag agtaacttca ggaataatgg tgtacatatc
     8521 cccttgctgg gtcgtattaa aggttatctg gctggaaagt gccgcaagga gtaataacgc
     8581 attcatattc ttctcccgaa aaaaaacagg gcttgcgccc tgtttctgta atacaaatga
     8641 tgtattaata ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtc acgttgacgg
     8701 aggagttcga tgcagtctgg tcaactgcag caccgttgcc accaccgaac tgtttaacag
     8761 tcatttcaga atttttgccg ttccactgat caagagtggc gctgttaccg aagccacgtt
     8821 gggtcagatc gattgagctg tcatctgagc cctgaccaac atctgcacca ttaccaccgc
     8881 catgctgggt aatagtcaag tcagagttac gggcatcagt ttgcagagca agtgcagagt
     8941 taccgccacc gtactggtaa atgttcagct ctgaatttgg gccgctatta tta
//
LOCUS       DADLTY010000168         8979 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.169, whole genome shotgun sequence.
ACCESSION   DADLTY010000168 DADLTY010000000
VERSION     DADLTY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.169"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..373
                     /gene="atpF"
                     /locus_tag="JN334_003415"
     CDS             <1..373
                     /gene="atpF"
                     /locus_tag="JN334_003415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAY7439445.1"
                     /translation="EKRQKEIADGLASAERAHKDLDLAKASATDQLKKAKAEAQVIIE
                     QANKRRSQILDEAKAEAEQERTKIVAQAQAEIEAERKRAREELRKQVAILAVAGAEKI
                     IERSVDEAANSDIVDKLVAEL"
     gene            388..921
                     /gene="atpH"
                     /locus_tag="JN334_003416"
     CDS             388..921
                     /gene="atpH"
                     /locus_tag="JN334_003416"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAY7439446.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            934..2475
                     /gene="atpA"
                     /locus_tag="JN334_003417"
     CDS             934..2475
                     /gene="atpA"
                     /locus_tag="JN334_003417"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAY7439447.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            2526..3389
                     /gene="atpG"
                     /locus_tag="JN334_003418"
     CDS             2526..3389
                     /gene="atpG"
                     /locus_tag="JN334_003418"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAY7439448.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            3416..4798
                     /gene="atpD"
                     /locus_tag="JN334_003419"
     CDS             3416..4798
                     /gene="atpD"
                     /locus_tag="JN334_003419"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAY7439449.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            4819..5238
                     /gene="atpC"
                     /locus_tag="JN334_003420"
     CDS             4819..5238
                     /gene="atpC"
                     /locus_tag="JN334_003420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAY7439450.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            5589..6959
                     /gene="glmU"
                     /locus_tag="JN334_003421"
     CDS             5589..6959
                     /gene="glmU"
                     /locus_tag="JN334_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAY7439451.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            7121..8950
                     /gene="glmS"
                     /locus_tag="JN334_003422"
     CDS             7121..8950
                     /gene="glmS"
                     /locus_tag="JN334_003422"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAY7439452.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         2189 a         2284 c         2484 g         2022 t
ORIGIN      
        1 cgaaaaacgt caaaaagaaa ttgctgacgg ccttgcttcc gcagaacgag cacataagga
       61 ccttgacctt gcaaaggcca gcgcgaccga ccagctgaaa aaagcgaaag cggaagccca
      121 ggtaatcatc gagcaggcga acaaacgccg ctcgcagatt ctggacgaag cgaaagctga
      181 ggcagaacag gaacgtacta aaatcgtggc ccaggcgcag gcagaaattg aagccgagcg
      241 taaacgtgcc cgtgaagagc tgcgtaagca agttgctatc ctggctgttg ctggcgccga
      301 gaagatcatc gaacgttccg tggatgaagc tgctaacagc gacatcgtgg ataaacttgt
      361 cgctgaactg taaggaggga ggggctgatg tctgaattta ttacggtagc tcgcccctac
      421 gccaaagcag cttttgactt tgccgtcgaa caccaaagtg tagaacgctg gcaggacatg
      481 ctgacgtttg ccgccgaggt aaccaaaaac gaacaaatgg cagagcttct ctctggcgcg
      541 cttgcgccag aaacgctcgc cgagtcgttt atcgcagttt gtggtgagca actggacgaa
      601 aacggtcaga acctgattcg ggtaatggct gaaaatggtc gtcttaacgc gctcccggat
      661 gttctggagc agtttattca ccttcgtgcc gtgagtgagg ctaccgctga ggtagacgtc
      721 atttccgctg ccgcactgag tgaacaacag ctcgcgaaaa tttctgctgc gatggaaaaa
      781 cgtctgtcac gcaaagttaa gctgaattgc aaaatcgata agtctgtaat ggctggcgtt
      841 atcatccgat cgggtgatat ggtcattgat ggcagcgtac gcggtcgtct tgagcgcctt
      901 gcagacgtct tgcagtctta aggggactgg agcatgcaac tgaattccac cgaaatcagc
      961 gaactgatca agcagcgcat tgctcagttc aatgttgtga gtgaagctca caacgaaggt
     1021 actattgttt ctgtaagtga cggtgttatc cgcattcacg gcctggccga ttgtatgcag
     1081 ggtgaaatga tctccctgcc gggtaaccgt tacgctatcg cactgaacct cgagcgcgac
     1141 tctgtaggtg cggttgttat gggtccgtac gctgaccttg ccgaaggcat gaaagttaag
     1201 tgtactggcc gtatcctgga agttccggtt ggccgtggcc tgctgggccg tgtggttaac
     1261 actctgggtg caccaatcga cggtaaaggt ccgctggatc acgacggctt ctctgctgta
     1321 gaagcaatcg ctccgggcgt tatcgaacgt cagtccgtag atcagccggt acagaccggt
     1381 tataaagccg ttgactccat gatcccaatc ggtcgtggtc agcgtgaatt gatcatcggt
     1441 gaccgtcaga caggtaaaac cgcactggct atcgatgcca tcatcaacca gcgcgattcc
     1501 ggtatcaaat gtatctatgt cgctatcggc cagaaagcgt ccaccatttc taacgtggta
     1561 cgtaaactgg aagagcacgg cgcactggct aacaccatcg ttgtggtagc aaccgcgtct
     1621 gaatccgctg cactgcaata cctggcaccg tatgccggtt gcgcaatggg cgaatacttc
     1681 cgtgaccgcg gtgaagatgc gctgatcatt tacgatgacc tgtctaaaca ggctgttgct
     1741 taccgtcaga tctccctgct gctccgtcgt ccgccaggac gtgaagcatt cccgggcgac
     1801 gttttctacc tccactctcg tctgctggag cgtgctgcac gtgttaacgc cgaatacgtt
     1861 gaagccttca ccaaaggtga agtgaaaggg aaaaccggtt ctctgaccgc actgccgatt
     1921 atcgaaactc aggcgggtga cgtttctgcg ttcgttccga ccaacgtaat ctccattacc
     1981 gatggtcaga tcttcctgga aaccaacctg ttcaacgccg gtattcgtcc tgcggttaac
     2041 ccgggtattt ccgtatcccg tgttggtggt gcagcacaga ccaagatcat gaaaaaactg
     2101 tccggtggta tccgtaccgc tctggcacag tatcgtgaac tggcagcgtt ctctcagttt
     2161 gcatccgacc ttgacgatgc aacacgtaag cagcttgacc acggtcagaa agtgaccgaa
     2221 ctgctgaaac agaaacagta tgcgccgatg tccgttgcgc agcagtctct ggttctgttc
     2281 gcagcagaac gtggttacct ggcggatgtt gaactgtcga aaattggcag cttcgaagcc
     2341 gctctgctgg cttacgtcga ccgtgatcac gctccgttga tgcaagagat caaccagacc
     2401 ggtggctaca acgacgaaat cgaaggcaag ctgaaaggca tcctcgattc cttcaaagca
     2461 acccaatcct ggtaacgtct ggaggcttgc cttagggcag gccgcaaggc attgaggaga
     2521 agctcatggc cggcgcaaaa gagatacgta gtaagatcgc aagcgtccag aacacgcaaa
     2581 agatcactaa agcgatggag atggtcgccg cttccaaaat gcgtaaatcg caggatcgca
     2641 tggcggccag ccgtccttat gcagaaacca tgcgcaaagt gattggtcac cttgcacacg
     2701 gtaatctgga atataagcac ccttacctgg aagaccgcga cgttaaacgc gtgggctacc
     2761 tggtggtgtc gaccgaccgt ggtttgtgcg gtggtttgaa cattaacctg ttcaaaaaac
     2821 tgctggcgga aatgaagacc tggaccgaca aaggcgttca atgcgacctc gcaatgatcg
     2881 gctcgaaagg cgtgtcgttc ttcaactccg tgggcggcaa tgttgttgcc caggtcaccg
     2941 gcatggggga taacccttcc ctgtccgaac tgatcggtcc ggtaaaagtg atgttgcagg
     3001 cctacgacga aggccgtctg gacaagcttt acattgtcag caacaaattt attaacacca
     3061 tgtctcaggt tccgaccatc agccagctgc tgccgttacc ggcatcagat gatgatgatc
     3121 tgaaacataa atcctgggat tacctgtacg aacccgatcc gaaggcgttg ctggataccc
     3181 tgctgcgtcg ttatgtcgaa tctcaggttt atcagggcgt ggttgaaaac ctggccagcg
     3241 agcaggccgc ccgtatggtg gcgatgaaag ccgcgaccga caatggcggc agcctgatta
     3301 aagagctgca gttggtatac aacaaagctc gtcaggccag cattactcag gaactcaccg
     3361 agatcgtctc gggggccgcc gcggtttaaa caggttattt cgtagaggat ttaagatggc
     3421 tactggaaag attgtccagg taatcggcgc cgtagttgac gtcgaattcc ctcaggatgc
     3481 cgtaccgcgc gtgtacgatg ctcttgaggt gcaaaatggt aatgagcgtc tggtgctgga
     3541 agttcagcag cagctcggcg gcggtatcgt gcgtaccatc gcaatgggtt cctccgacgg
     3601 tctgcgtcgc ggtctggatg taaaagacct cgaacacccg atcgaagtcc cggtaggtaa
     3661 agcgactctg ggccgtatca tgaacgtact gggtgaaccg gtcgacatga aaggcgagat
     3721 cggtgaagaa gagcgttggg cgattcaccg cgcagcacct tcctacgaag agctgtcaaa
     3781 ctctcaggaa ctgctggaaa ccggtatcaa agttatcgac ctgatgtgtc cgttcgctaa
     3841 gggcggtaaa gttggtctgt tcggtggtgc gggtgtaggt aaaaccgtaa acatgatgga
     3901 gctcattcgt aacatcgcga tcgagcactc cggttactct gtgtttgcgg gcgtaggtga
     3961 acgtactcgt gagggtaacg acttctacca cgaaatgacc gactccaacg ttatcgacaa
     4021 agtatccctg gtgtatggcc agatgaacga gccgccggga aaccgtctgc gcgttgctct
     4081 gaccggtctg accatggctg agaaattccg tgacgaaggt cgtgacgttc tgctgttcgt
     4141 tgacaacatc tatcgttaca ccctggccgg tacggaagta tccgcactgc tgggccgtat
     4201 gccttcagcg gtaggttatc agccgaccct ggcggaagag atgggcgttc tgcaggaacg
     4261 tatcacctcc accaaaactg gttctatcac ctccgtacag gcggtatacg tacctgcgga
     4321 tgacttgact gacccgtctc cggcaaccac ctttgcgcac cttgacgcaa ccgtggtact
     4381 gagccgtcag atcgcgtctc tgggtatcta cccggccgtt gacccgctgg actccaccag
     4441 ccgtcagctg gacccgctgg tggttggtca ggaacactac gacaccgcgc gtggcgttca
     4501 gtccatcctg caacgttatc aggaactgaa agacatcatc gccatcctgg gtatggatga
     4561 actgtctgaa gaagacaaac tggtggtagc gcgtgctcgt aagatccagc gcttcctgtc
     4621 ccagccgttc ttcgtggcag aagtattcac cggttctccg ggtaaatacg tctccctgaa
     4681 agacaccatc cgtggcttta aaggcatcat ggaaggcgaa tacgatcacc tgccggagca
     4741 ggcgttctac atggtcggtt ccatcgaaga agctgtggaa aaagccaaaa aactttaacg
     4801 ccttaatcgg agggtgatat ggcaatgact taccacctgg acgtcgtcag cgcagagcaa
     4861 caaatgttct ctggtctggt cgagaaaatc caggtaacgg gtagcgaagg tgaactgggg
     4921 atctaccctg gccacgcacc gctgctcacc gccattaagc ctggtatgat tcgcatcgtg
     4981 aaacagcacg gtcacgaaga gtttatctat ctgtctggcg gcattcttga agtgcagcct
     5041 ggcaacgtga ccgttctggc cgacaccgca attcgcggcc aggatctcga cgaagcgcga
     5101 gccatggaag cgaaacgtaa ggctgaagag cacattagca gctctcacgg cgacgtagat
     5161 tacgctcagg cgtctgcgga actggccaaa gcgatcgcgc agctgcgcgt tatcgagttg
     5221 accaaaaaag cgatgtaaca ccggcttgaa aagcacaaaa gccagtctgg aaacaggctg
     5281 gcttttttta cgcgttgacc cgtcctgaag agcgttcaca tagatcctgc tgatataaaa
     5341 cacccctgtt ttcccgttta ttcattgatc gaaataagag caaaaacatc cacctgacgc
     5401 ttaaatgaag gtactgcctt aattttctgc agataaaagg cgtgacgatg gtcgaaaatg
     5461 gcactttcgt cagcggggat aatccgttat tgaacaattt atcctctgtc catttcacga
     5521 tgaaaaaaat gtagtttttt caaggtgaag cggtttaaat tcgttctcaa attacagtca
     5581 ggacgcgtat gttgaataat gctatgagcg tagtgatcct tgccgcaggc aaaggcacgc
     5641 gcatgtattc cgatcttccg aaagtgctgc atacccttgc cgggaaagcg atggttcagc
     5701 atgtcattga tgctgcgaat gaattaggcg cagcgcacgt tcacctggtg tacggtcacg
     5761 gcggcgatct gcttaaacag gcgctgaaag acgacaacct taactgggtg cttcaggcag
     5821 agcagctggg tacgggtcat gcaatgcagc aggccgcacc tttctttgcc gatgatgaag
     5881 acattttaat gctctacggc gacgtgccgc tgatctctgt cgaaacactc cagcgcctgc
     5941 gtgatgctaa accccagggt ggcattggtc tgctgacggt gaaactggat gatccgaccg
     6001 gttatggacg tatcacccgt gaaaatggca aagttaccgg cattgttgag cacaaagatg
     6061 ccaccgacga gcagcgtcag attcaggaga tcaacaccgg cattctgatt gccaacggcg
     6121 cagatatgaa acgctggctg gcgaagctga ccaacaataa tgctcagggc gaatactaca
     6181 tcaccgacat tattgcgctg gcgtatcagg aagggcgtga aatcgtcgcc gttcatccgc
     6241 aacgtttaag cgaagtagaa ggtgtgaata accgcctgca actctcccgt ctggagcgcg
     6301 tttaccagtc cgaacaggct gaaaaactgc tgttagcagg cgttatgctg cgcgatccgg
     6361 cgcgttttga tctgcgcggt acgcttactc acgggcgcga tgttgaaatt gatactaacg
     6421 ttatcatcga gggcaacgtg actctcggtc atcgcgtgaa aatcggcacc ggttgcgtga
     6481 ttaaaaacag cgtgattggc gatgattgcg aaatcagtcc gtataccgtt gtggaagacg
     6541 cgaatctggc agcggcctgt actattggcc cgtttgcccg tctgcgtcct ggtgctgagt
     6601 tgctggaagg tgcacacgtt ggtaacttcg ttgagatgaa aaaagcgcgt ctgggtaaag
     6661 gcacgaaagc cgggcatctg acttacctgg gcgatgcgga aattggcgat aacgttaaca
     6721 tcggcgcggg aaccattacc tgcaactacg atggtgcgaa taaatttaag accattatcg
     6781 gcgacgatgt gtttgtcggt tccgacactc agctggtggc cccggtaaca gtaggcaaag
     6841 gcgcgaccat tgctgcgggt acaactgtga cgcgtaatgt cggcgaaaat gcattagcta
     6901 tcagccgtgt gccgcagact cagaaagaag gctggcgtcg tccggtaaag aaaaagtaat
     6961 tctggccggg taatccggtc atctgggatg aggagataac ataatctccc tcccacaagc
     7021 agtaactata aaaataaccc cactctctac aaggctcggg gcgcccgaaa aaacgggcat
     7081 acaggttgac cgacaacgat ataaatcgga atcaaaaact atgtgtggaa ttgttggcgc
     7141 gatcgcgcaa cgtgatgtag cagaaatcct tcttgaaggt ttacgtcgtc tggaataccg
     7201 cggatatgac tctgccggtc tggccgttgt tgatgcggaa ggtcatatga ctcgcctgcg
     7261 tcgcctcggt aaagtccaga tgctggctca ggcagcggaa gaacatcctc tgcatggcgg
     7321 caccggtatt gctcacactc gctgggcgac ccacggtgaa ccttcagaag ctaacgcgca
     7381 tccgcatgtt tctgaacaca ttgtggtggt gcataatggc atcatcgaaa accatgaacc
     7441 gctgcgtgaa gagttaaaag cgcgtggcta taccttcgtt tctgaaaccg acaccgaagt
     7501 gattgcccat ctggtgaact gggagctgaa acaaggcggg actctgcgtg aggctgttct
     7561 gcgtgctatc ccgcagctgc gcggtgcgta cggtacagtg atcatggact cccgtcaccc
     7621 ggataccctg ctagcggcac gttctggtag tccgctggtg attggcctgg gtatgggcga
     7681 aaactttatc gcttctgacc agctggcgct gttgccggtg acccgtcgct ttatcttcct
     7741 tgaagagggc gatattgcgg aaatcactcg ccgttcggta aacatcttcg ataaaactgg
     7801 cgcggaagta aaacgtcagg atatcgaatc caatctgcaa tatgacgcgg gcgataaagg
     7861 tatttaccgt cactacatgc agaaagagat ctacgaacag ccgaacgcga taaaaaacac
     7921 ccttaccggg cgcatcagcc acggtcaggt tgatttaagc gagctgggac cgaacgccga
     7981 cgaactgctg tcgaaggttg agcatattca gatcctcgcc tgtggtactt cttataactc
     8041 cggtatggtt tcccgctact ggtttgaatc gctggcaggt attccgtgcg acgtcgaaat
     8101 cgcctctgaa ttccgctatc gtaaatctgc cgtgcgtcgt aacagcctga tgatcacctt
     8161 gtcacagtct ggcgaaaccg cggataccct ggcgggcctg cgtctgtcga aagagctggg
     8221 ttaccttggt tcgctggcaa tctgtaacgt tccgggttct tctctggtgc gcgaatccga
     8281 tctggcgctg atgaccaacg cgggtacgga aatcggcgtg gcgtccacta aagcattcac
     8341 cactcagtta actgtgctgt tgatgctggt ggcgaagctg tctcgcctga aaggtctgga
     8401 tgcctccatt gaacatgaca ttgtgcatgg tctgcaggcg ctgccgagcc gtattgagca
     8461 gatgctgtct caggataaac gcattgaagc tctggcagaa gatttctctg acaaacatca
     8521 cgcgctgttc ctgggccgtg gcgatcagta cccaatcgcg ctggaaggcg cattgaagct
     8581 gaaagagatc tcttacattc acgctgaagc ctacgctgca ggcgagctga aacacggccc
     8641 gctggcgctg attgacgcgg atatgccggt tatcgtcgtt gcaccgaaca acgaactgtt
     8701 ggaaaaactg aaatccaaca ttgaagaagt tcgcgcgcgt ggcggtcaat tgtatgtctt
     8761 cgccgatcag gatgcgggtt ttgtaagtag cgataacatg cacatcatcg agatgccgca
     8821 tgtggaagag gtgattgcac cgatcttcta caccgttccg ctgcagctgc tggcttacca
     8881 tgtcgcgctg atcaaaggca ccgacgttga ccagccgcgt aacctggcaa aatcggttac
     8941 ggttgagtaa tcactatcat agataaaaaa gggctgccg
//
LOCUS       DADLTY010000169         8838 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.170, whole genome shotgun sequence.
ACCESSION   DADLTY010000169 DADLTY010000000
VERSION     DADLTY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.170"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(158..1129)
                     /locus_tag="JN334_003423"
     CDS             complement(158..1129)
                     /locus_tag="JN334_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HAY7439453.1"
                     /translation="MKRAPVIPRHTTHSQSTEDTSSPAPAAPMVDSLIARVGAMARGN
                     AISLPVCGREVKFTLEVLRGDSVESASRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSDVIEIADGSRRRKAAILTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGLRYTSRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFAHPGELSA
                     RSGEALQKAFADKEELLKQQAETLHDQKKAGLIFEAEEVISLLTSVLKQSPASRVNLS
                     SRHQFAPGATALYKGDKMVLNLDRSRIPAECIEKIEAILKELEKPGV"
     gene            complement(1129..2295)
                     /gene="sopA"
                     /locus_tag="JN334_003424"
     CDS             complement(1129..2295)
                     /gene="sopA"
                     /locus_tag="JN334_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HAY7439454.1"
                     /translation="MGLMDTLNQCISAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMETRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DAFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDASYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPA
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNNNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            3036..4046
                     /locus_tag="JN334_003425"
     CDS             3036..4046
                     /locus_tag="JN334_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7439455.1"
                     /translation="MTSENNSLLLNLQEVDKTTGEVVKLDVNSTSTVQPVALMRLGLF
                     VPTLKSTGKSKANRKNVTDATEELVQLAIAKSEGYTDVKITGSRLDMDTDFKVWLGII
                     RSMSEYGVKSDTLELSFVEFVKMCGFDSRRSNKKMRDRISNSLFKLASVTLKFQSETK
                     GWTTHLVQSAYYDINEDIVEIKAEPKLFELYHMDRRVLLRLKAIDALQRKESAQALYT
                     YIESLPQNPAPISMKRMRDRLNLTSNVYTQNHTVRKAMEQLRDIGYLDYTEFKRGRAT
                     YFSVHYRNPKLISSPVKVPRKEEEEKAPEQNYDEVIKALKAAGIDPLKLAEALSAMKP
                     EN"
     gene            complement(4852..5181)
                     /locus_tag="JN334_003426"
                     /pseudo
     CDS             complement(4852..5181)
                     /locus_tag="JN334_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5299..6024
                     /locus_tag="JN334_003427"
     CDS             5299..6024
                     /locus_tag="JN334_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY7439456.1"
                     /translation="MAYSIGEFARLSGITATTLRAWQRRYGLLKPQRTEGGHRQYSDE
                     DVQQALKILDWVKKGVPIGQVKPLLERPTSRLTNNWLTLQQSMLQRLQDGKIESLRQM
                     IYDAGREYPRPELVANVLRPLRSQVSANVAAAMTLREILDGIIIAYTSFCLEGDKKAP
                     GDNILLSGWNLNDPCEVWLEALTRTGQGHRIDILPTPPATLAPEIFPDRHWLLVTRGK
                     LTAARKKQIEQWQQQVSLEVIIL"
     gene            6124..6534
                     /gene="uraH"
                     /locus_tag="JN334_003428"
     CDS             6124..6534
                     /gene="uraH"
                     /locus_tag="JN334_003428"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY7439457.1"
                     /translation="MKKTASLLMLTTLAFAPAAFSAPAGTLSVHILDQQTGMPPSDVT
                     VTLEKQQQDKWTPIASGKTDHDGRIKSLYPEDQDMQPGVYKVAFKTADYFHGKKLDSF
                     FPEIPVLFTVTRTNEKLHIPLLLSQYGYSTYKGS"
     gene            6637..7595
                     /locus_tag="JN334_003429"
                     /pseudo
     CDS             6637..7595
                     /locus_tag="JN334_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208729.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
     gene            7741..8829
                     /locus_tag="JN334_003430"
     CDS             7741..8829
                     /locus_tag="JN334_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7439458.1"
                     /translation="MITTLHIQNYRSIREMSLELEQLNIVFGPNGTGKSNIYKAIHLM
                     HSAAQGQFSQALANEGGILKVFWAGKTRSDQLRRMNLAVETETYEYELQVGFVEKLPY
                     PSQFQLDPVIKEESIWLSGQYRRPSSQLMKRKNQAVFLNNVHHEKVTHSGTLYENESV
                     FGQLGEPHLYPEVSQMRESLRNWRFYQEFSVSIGSAMRAPQVGFRSPVLASDGANLAA
                     AFQTIVEIGDELLLMRILDQAFPGCVFYSDNTGGRFRMMMQREGLSRPLEPAEFSDGT
                     LRFLCLAVALLSPRPPAFIALNEPENSLHPQMLPALASLIAEASRYSQIWLTSHSPEL
                     ANLIEKQRSFSLYQLSMVEGETKVERLG"
BASE COUNT         2235 a         2336 c         2253 g         2014 t
ORIGIN      
        1 cgacgcaggt gggaccgtgg tcccagggag ataacctgag cggcgataca ggtgggaccg
       61 tggtcccaga gagaatattc aggccagtta tgttttctgg cctgtgagaa aggaaatgaa
      121 gtaaagggag atcaccgtag actaaagcgt gtccgcatca gactcctggt ttctcaagct
      181 ccttaagaat ggcttctatt ttctctatac actccgcagg aatgcgggac ctatccaggt
      241 taagcaccat tttatcgccc ttatacaatg ctgttgcccc tggagcaaac tgatgacgtg
      301 agctcagatt aactcttgat gcgggagatt gtttcagtac ggatgttaaa agactaatga
      361 cctcttcagc ttcaaagatt agtcccgctt tcttctgatc gtggagagtc tcagcctgct
      421 gcttcagtaa ttcttcttta tctgcaaaag ccttctgaag ggcttcacct gaccgggcag
      481 acagttctcc aggatgtgca aacagggcaa caacggattt aggcaatttg gccgtattga
      541 tacagcgggt aatgatctta cgagagatgt tttccgcatc ggcaagcgca gaaatatttc
      601 ctgcaaactc attctgcagc cggcttgtat aacgcaggcc acgttcataa gcactcgtcg
      661 gccgataatc gttacccagt cgggacaatg cagccatctg ctcatcgtcc agttcaccaa
      721 ccagaacgcg ataatcactt tccgtcagga ttgcggcttt acggcgacgg ctgccgtcgg
      781 caatttctat gacatcagaa acccggcggc cgaaagctgg agtctgctga ccggtcagta
      841 aaaatgaagg aatgagatca tccagagcat cttcggtaag tagttcctga tcgcgctcat
      901 tgcctgacca tacgcgtgaa gcgctctcaa cagagtctcc ccggagcacc tcaagggtaa
      961 atttcacttc ccgtccacat accggaagag agattgcatt accgcgggcc atagcaccca
     1021 cgcgcgcaat taatgaatcc accatcggcg cggccggcgc cggtgatgaa gtatcttcag
     1081 tcgattgact atgtgtggta tgtcttggaa tgacaggagc acgcttcatt atctaatctc
     1141 ccagcgtggt ttaatcagac gatcaaaaat ctcattgcaa acaggctccc agatagagag
     1201 ggcattacgc caggcaccgg ttgaagaacg ttgatcaata gcctgttcaa aaacggttct
     1261 catacggatc tgacctttac cgacttcatc cgtttcgcgc acaacatttt taaggaccat
     1321 gcttccccag gcatcccgaa tttgttcttc catccacggg gactgtgagc cattattatt
     1381 actgtatttg gtaagcagaa tacggacatc tggctcgaag cctttaagat ccacgttctt
     1441 aagcaggtca cgaagcatat cgaaaaactg cagtgcggag gtgtagtcga acagctctgc
     1501 cggagtaggg acaatcagaa catcagcagc gcatacaaca tttatcgtac cgataccaag
     1561 gttgggtgca ctgtcgataa ctatcacgtc ataatcatga gcgacagttt caatggccag
     1621 acggagcatc aggtgcgggt cagcaggtaa tttgccttcg tcaaatttcc ccatcagctc
     1681 ggtttcaatg cggtgcagtg ccagacagga cgggatgata tcaaggccag gccagcaggt
     1741 gggctttata gcgtagctgg cgtcatcctt ttccccaaga taaaagggca ggagagtgtc
     1801 ctcagcatga atatgaagat cgggcaccca tccgtgatac atagaagcag ttccctgtgg
     1861 gtcattgcct tcaacaagca gaacacggag tcctttcaga gcaagatcct gagccaggtg
     1921 aacggatact gaggttttgt aaacaccacc tttgtgagct gcaacgccaa tcaccggcgg
     1981 aaacgcatct tcagcacggc gcagtcgtgt accaaataca tcccgcatgt gattgatttg
     2041 ttcgatggta taaccaacgc gttgctcaac tctcccacgg gtttccatat cagggtgcgg
     2101 cagccggcca gctttctcgg catccctgat agcctgagat gacacgccaa ctaaatctgc
     2161 cgcttcacca atcctccagc gccgggttat tttccgggct tccggactgt catcattaaa
     2221 ctgtgcaatg gcgattgctt tcgtcatttc atgaccagca cttatgcact ggttgagtgt
     2281 atccatgagt cccatactga acatccttta atcatcactt tgcattattt tattaaatct
     2341 tggaatttat tgcaaagcaa caataaaatc gcaaagcaca cgaaaaagtg caaagtgaac
     2401 cacattgggt aatgaaagaa cgcaggggat tacaaaagag tacataccgc agtcaatatt
     2461 aatcactgcc gctcgccgca gtaagcgatc tcacacagtg agaaagcggc gaggaagcgg
     2521 aaaatcacag aactcacaga ttgcacgtac gtcgggttgc taaagatgac tcgggatcag
     2581 ccagttataa tgaaattgac tgcggtatgt aatatattga ctatggtatg atgcttaccg
     2641 catctgtagg aaagacgact cagcataccg cagtcaatgt ttcattgttg tacctgtaga
     2701 tctcaatctt ccagagcgta ttcaggaaaa tcaggccagg ggtctggata tctggtgtgg
     2761 cgcttccggc cttgtatctg ggttattcat ggtctcgatc caataattga tcccaaggag
     2821 atcatagtga gatccaaaag agatccccgg atcgctccag catgcgctgt aactggcttt
     2881 aacgtctgtc tggatcgact gcggaatgta aaatgttgac tgttatatgc ataatctaat
     2941 actgtgatat gcaacggtat ttactgaggt atgtatatcc gttgactgcg atacgtatta
     3001 ctacatcctt ttgtggataa ccgttgagga tcaagatgac gagcgaaaat aatagcttac
     3061 ttctgaacct tcaggaagtt gataagacaa ccggtgaagt tgttaagctg gatgtcaaca
     3121 gcaccagtac cgtgcagcca gtagcgctca tgagactcgg tctcttcgtc ccaactctga
     3181 aatcaacagg gaagagcaag gcgaaccgga aaaacgttac agacgcgact gaggagcttg
     3241 tacagctggc cattgccaaa agcgaaggat acactgacgt taagatcacc ggttcgcgtc
     3301 ttgatatgga cacggatttt aaagtctggc tggggataat tcgctccatg tcggagtatg
     3361 gggtaaaaag tgacaccctg gaactgtcgt tcgtcgaatt cgttaagatg tgcggatttg
     3421 actcccgtcg ttcaaacaaa aaaatgcgcg atcgcatcag taattccctg tttaaactcg
     3481 cctcggttac gctgaagttc cagagcgaga ccaaaggatg gaccacccat ctggtgcagt
     3541 cagcctatta tgacatcaac gaggatatcg ttgagatcaa ggctgaacct aagctgtttg
     3601 agctttacca catggacaga agggtactgc tgcggctgaa agccattgac gccctgcagc
     3661 gcaaggaatc agcccaggcg ctttatacct acatcgaaag tcttccccag aatcctgcac
     3721 ctatttccat gaaacggatg cgtgaccggc tgaatctcac ctcaaatgtc tatacacaga
     3781 accacacggt gcgtaaggcg atggagcagc tgcgtgatat tggttatctg gattacaccg
     3841 agtttaaacg tggccgagcc acctatttca gcgtgcatta tcgcaacccc aaactcatca
     3901 gtagtcctgt gaaggttccg cgcaaagagg aagaagagaa ggcgccagag caaaattatg
     3961 acgaagtcat taaggcgctg aaggctgctg gcatcgatcc tcttaaactg gctgaagccc
     4021 tctcagcgat gaagccagaa aattgactgc gatatgcata tcatgactgc cgtatgccgc
     4081 taaggacatt ccattgactg tgatatgcat cacgcatacc gcagtcaata tttattctct
     4141 atcatgtctg cccggatcga acaatattga ctgtcatatg tgatttcggg tcatgatacg
     4201 cagatattga ctacggaatg cgggtttaat gggggtaatc cctggtctgt tcaccattcc
     4261 atgggggata acatgccaca gtcaattttt cagtaacgct ctatatccct tacgaaaatc
     4321 tcaatcgggt gctgagacgg tcatgttacc tgacggtatc cgttcccctg tgtactgcag
     4381 gtttagcccg gcaggatgag actgcagctg tctgtataac ccggcgccag cttcacactg
     4441 ttaaatgcga tcaggaaacg ggagccgaat ggcagaaagg tgttggctag ggaaggtgcg
     4501 aataagcagg ttatttcttc ccaagctgac tcgctgatta aaatttcgcg gatctgggcc
     4561 gatttttttc ccgcaaacac atcgaatcag cctatttagg ggtcagtttg gagatcggaa
     4621 atttttgtac gttaagcaca ttatttaacc aattcggata cgtaagtgat tgatttatcg
     4681 tcattggcta tcgcttttag gttgtcgctc ttgctgtatc gcatttaggt tatccgatta
     4741 agacaaaatc ccctgaaaac gctgtatagc gcgggagtac accctattgc tacaggtaag
     4801 cctgttcaaa aaacaggctt accgtacaat aattctctat atccaaactg accccaagaa
     4861 ggacaccttc agcgcggccg tggtcggttc gccggtaaag taggctttac cctcgctctc
     4921 attccacttg ttcttcacag ggtcgtagcc gcggttaagc acgctgatgc cgccgtcgct
     4981 gcgcttgccg taggtggcgg taacctgctc taggccgcgt tcaaagcggt tttccaggcg
     5041 agcgacttcg taccatttgc caagatagcg gctggcgtca aaaccggaaa tgggctgcac
     5101 gcccttcggt ggggtagggg atttacaggc taccagcgtc agggcaatgg caacaccggt
     5161 cacaacaggc catagcttca tgagaattcc tttcatttgc acggttggaa attaagtgta
     5221 gtgcaccgcg gcggcgcttc atcccatccg cgaaaattcg tagtatattg agagtattcc
     5281 tcaactctgg acacggacat ggcttactcc atcggcgaat tcgccagact cagcgggatc
     5341 accgccacca cgctgcgggc ctggcagcgg cgctacggct tactcaagcc tcagcgcacg
     5401 gagggcggcc accgccagta cagcgatgag gacgtccagc aggcgctaaa aatcctcgac
     5461 tgggtaaaaa aaggcgtccc gattggccag gtcaaaccgc tgctggagcg ccctacgtcg
     5521 cgcctgacca acaactggct cacgctgcag cagagcatgt tacagcgcct gcaggacggc
     5581 aaaatcgagt ccctgcgtca gatgatttac gacgcaggac gcgagtaccc aagaccagaa
     5641 ctggtcgcga acgtgctgcg tccgctgcgc agtcaggtgt cggcaaacgt cgccgccgcc
     5701 atgaccctgc gggagatcct cgacggcatc atcatcgcct acacctcgtt ttgcttggaa
     5761 ggcgataaaa aagcgccggg cgacaacatc ctgctcagcg gctggaacct gaatgacccg
     5821 tgcgaagtct ggctcgaagc cttaacccgc accgggcaag gccaccgtat cgatatttta
     5881 cccacgccgc ccgccacgct ggccccggag attttcccgg accgccactg gcttctggtc
     5941 acgcgcggta aactcaccgc cgcgcgcaaa aagcagatcg agcagtggca gcagcaggta
     6001 tcgcttgaag tgattatcct ctaatcattt tctcctgcgg gaatcttcac gaaaagttga
     6061 atattttccc gcgccacaaa tgctaacgtt ttcctgtgac cgacaacccc acaggaaaac
     6121 acaatgaaaa aaactgcctc tctgctgatg ctgaccaccc tggcctttgc ccctgccgcc
     6181 tttagcgccc ctgccggcac gctgagcgta cacattcttg accagcaaac cgggatgccg
     6241 ccatcggacg ttaccgtcac gctggaaaaa cagcagcagg acaaatggac cccaattgcc
     6301 agcggcaaaa ctgaccatga cggccgcatc aaatcgcttt atccggagga tcaggatatg
     6361 cagcccggcg tctataaagt ggcctttaaa accgcagatt acttccacgg gaagaagctg
     6421 gactccttct tcccggagat cccggtgctg ttcaccgtaa cccggaccaa cgaaaagctg
     6481 cacatccctc tcctgctgag ccaatacggt tattcaactt acaagggcag ttaagaaacg
     6541 ttgattttaa aggcaaataa aaaataattc cgctaatact tggacaaatt agttatgttg
     6601 tgtaagtttt aaagagaaga acgggaggac cacaccatga caacaaaacc tgtgctcggt
     6661 attagcggat gtttgaccgg gtctgccgtt cgcttcgacg gcggacacaa gcgtatgggc
     6721 tttgtcatgg atgaactggc ccagtgggtg agtttcaggc ccgtctgccc ggaaatggcg
     6781 attggcctgc cgacgccacg cccggccata cgcctgacgc tgacggacag cggcgaaacg
     6841 cagctccgct tcagcaagcc gccccatgat gatataacgc agaaaatggc cgactttacg
     6901 gcggactatc tgccaaaaat cggcgatctg tcgggattta tcgtctgcgc gaaatcgcca
     6961 agctgcggca tggaacgcgt gcggctatat gatgaaaacg gcaaccgggg ccgcaaggag
     7021 gtgtcgggct gttcaccgcc gccctgcttg aaacctaccc gtggctgccc gtggaggaag
     7081 acggtcggct gcacgacccg gtgctgcggg aaaactttat cgagcgggta tttgccctgc
     7141 acgagctgaa cacgctgcgc gctaaaggcc tgacgcgccg cgcgctgctg gatttccaca
     7201 gccgctataa attgcagctt ctggcgcacc atcaggcggg ctaccgcgag attggcccgt
     7261 tcgttgcgtc gctgcatgaa tgggaagatc tggacgcgtt cttcgtggcg taccgcgaaa
     7321 agctgatgac catcctgaaa aagcccgcct cgcggaaaaa ccataccaac gtgctaatgc
     7381 atatccaggg ctatttccgt aaccagctga acagccgcca gcgcgtcgag ctgcgcgatg
     7441 tgatcctgca ctatcgcgac gggctgctac ccatcctcgc gccgttgacg ctactgaaac
     7501 actacatggc cgaataccct gaccggtatc tgatgacgca aaactacttt gatccctatc
     7561 ctgatgattt gggtctgcgt ctggcagtaa cctgacgata aaaatgcgaa ggcccgtttg
     7621 acccacagag gcccaaagca gtaccccgtt tagacgacac cctcactgtc gtctcttttt
     7681 tgcattccac acaaaagact gtaattatgt acaggattgt aacgcaaaaa gggactgcct
     7741 gtgatcacca ccctgcacat tcaaaactac cgctcgattc gcgaaatgtc gctggagctg
     7801 gaacagctca acatcgtctt tggcccaaac ggcaccggaa aatccaatat ctacaaggcg
     7861 atccacctga tgcacagcgc cgcacagggg cagttttcgc aggcactggc gaacgagggt
     7921 gggattttaa aagtgttctg ggcaggcaaa acccgcagcg accagctgcg gcggatgaat
     7981 ttggccgttg aaaccgaaac ctacgaatac gagctgcagg taggctttgt cgaaaaactg
     8041 ccttacccct cacagtttca actcgatccg gtgatcaaag aagagtccat ctggctgagt
     8101 ggtcaatacc gccgcccgtc gtcacagctg atgaagcgta aaaatcaggc cgtatttctg
     8161 aacaacgtgc atcacgagaa agtcacccac agcggcacgc tgtatgagaa cgaatcggtg
     8221 ttcggccagc ttggcgagcc gcatctttac ccggaagtgt cgcagatgcg cgagtcgctg
     8281 cgtaactggc gcttttatca ggagttttct gtatcaatcg gctcggccat gcgcgcgcct
     8341 caggtcggtt tccgctcccc agtgctggcc agcgacggcg caaacctggc ggcggcgttt
     8401 cagaccatcg tggaaattgg cgacgagctg ctgctgatgc gcatcctcga ccaggccttc
     8461 cccggctgcg tgttttacag cgacaacacc ggcggacgct tccggatgat gatgcagcgt
     8521 gaagggctaa gcagaccgct ggagccggcg gaattctccg acggcactct gcgttttctg
     8581 tgcctggcgg tggcgctatt aagcccgcgt ccaccggcct ttatcgccct caacgaaccg
     8641 gaaaacagcc tgcatccgca gatgctgccc gcgctggcga gcttaatcgc cgaggccagc
     8701 cgctactcgc aaatttggct gaccagccac tcgccggaac tggcgaattt aattgagaaa
     8761 cagagatcgt tttcgctgta tcagctgtcg atggtggaag gagaaacgaa ggtggagagg
     8821 ctggggtagt ctcccaga
//
LOCUS       DADLTY010000170         8759 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.171, whole genome shotgun sequence.
ACCESSION   DADLTY010000170 DADLTY010000000
VERSION     DADLTY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.171"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            7..438
                     /locus_tag="JN334_003431"
     CDS             7..438
                     /locus_tag="JN334_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439459.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="JN334_003432"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="JN334_003432"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAY7439460.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="JN334_003433"
     CDS             complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="JN334_003433"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HAY7439461.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="JN334_003434"
     CDS             complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="JN334_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAY7439462.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4440..5147)
                     /locus_tag="JN334_003435"
     CDS             complement(4440..5147)
                     /locus_tag="JN334_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HAY7439463.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="JN334_003436"
     CDS             complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="JN334_003436"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAY7439464.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6005..6208)
                     /locus_tag="JN334_003437"
     CDS             complement(6005..6208)
                     /locus_tag="JN334_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7439465.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6397..7548
                     /locus_tag="JN334_003438"
     CDS             6397..7548
                     /locus_tag="JN334_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439466.1"
                     /translation="MHYRNVVCLLSCSLFLSSAWGCRLSAPEHNIYQKQGKGVVYLRP
                     YKETNLSLPDVNYKSLRRLPNLLIDPTTLDEWDKEPPLTDLTTDYLYEGAQAWYPHYS
                     WHSDGRNILYAGEVVQNPPGTPPVDVASFKAWGDFAADKHSLYFEGKRTDDNGGGNSL
                     DIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPESFRVLAQKSWDQRGKFST
                     TFNPCIAVPFGPWDTLARTRTKILLNGEQLDADPDTFSVVRWMPGSLLTWRDKNGLQR
                     KVLDKENLAWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQFQPISDAVAQ
                     HQDSLYTIIETESGNRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAEG"
     gene            7570..7875
                     /locus_tag="JN334_003439"
     CDS             7570..7875
                     /locus_tag="JN334_003439"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439467.1"
                     /translation="MFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFD
                     GAQLWRYRTADPKQVRLIWKEQHSGYVYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            7872..>8759
                     /locus_tag="JN334_003440"
     CDS             7872..>8759
                     /locus_tag="JN334_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HAY7439468.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEP"
BASE COUNT         2033 a         2387 c         2250 g         2089 t
ORIGIN      
        1 cagaaggtgg cgttaaatct cgcttcaggt gaagggaatt tctttatccg tcctggcggc
       61 gtgttttatg tcgcgggaga taaagtcggc atcgttcgtc tggatgcttt taaagccagt
      121 aaagatattc agtttgcggt gcagtcagga ccaatgttga tggaaaacgg tgtaattaat
      181 ccgcgtattc atcccaacgt cgcctcacgt aaaattcgta acggtgttgg gattaataaa
      241 catgggaatg ccgtgttttt gttgagccag caggcgacaa atttttatga ttttgcctgt
      301 tatgccaaag cgaaactgaa cgttgagcag ctgctttatc ttggtggcac gatttcacat
      361 atgtatatga aaggcggggc aatcccgtgg caacgctatc cttttgtcac catgatttcc
      421 gtcgagcgga aaggttagaa tgatagcttt gtgtcagata ctaaatatgc tgctgatgcc
      481 ggatgcggcg taaacgcctt atccggccta catgacgttg caatttattg aatctgaatg
      541 attttttagg ccaaataagg tgcgcagcac cgcatccggc aacgttacac cgactccagc
      601 cgggcgtaag ctgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg
      661 ctatgttcac cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgt
      721 acacgctggc cgagcttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga
      781 tggctgaccg ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc
      841 aactcgccaa taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt
      901 tccgcgtagg tcagcgtcag tttctgcatc gcgcgggtta cgccaacgta ggccagacgg
      961 cgttcttctt ccagacgccc gccttcatcc agcgacatct ggcttgggaa catgccctct
     1021 tccataccga cgataaacac ctgcgggaac tccagacctt tcgccgagtg catggtcatc
     1081 agttgtaccg catcctgcca ggtatccgcc tgcccttcgc ctgcttccag cgccgcatgg
     1141 gataggaacg cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc
     1201 gttgccgtca ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc
     1261 tgctcgtaca tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc
     1321 ggcatatcgg cagtttcctg cgctaaggcg tcgatcagtt ccataaaccg ctgtaaggcg
     1381 ctggcagcac gtccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt
     1441 gttaactggc gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc
     1501 cgcgttggcg tattcaccac acgctcaaag gccgcgtcgt cgttacggtt ggcaatcagg
     1561 cgcagatacg agagcgcatc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa
     1621 atacggtacg gcatactcgc ctgcaacagt gcttcttcca gcacacgcga ctgggcgtta
     1681 ctgcggtaga gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg
     1741 cggttaacca caaaacgcgc ttcatcgagt tcgttaaaag cgcaatagag ggaaataggc
     1801 tcaccgtccg cgccatcggt ccacagtttt ttacccagac gcccgttatt gttttcaatc
     1861 agggcgttag cggcgctcag aatattgctg gtagagcggt agttttgctc cagacgaata
     1921 atttcggcac cggggaaatc attaaggaaa cgctgaatat tctccacctg cgccccgcgc
     1981 cagccgtaga ttgactggtc gtcatcaccg acgatcatca ctttgccggt gtcgcccgcc
     2041 agcaggcgga tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggata
     2101 ttggtaaaac gttcgcggta gtgttgtagg atatgcggct tgttaagcca cagttcgtga
     2161 gcgcgcagca gcagctcggc gaagtccacc aggcccgcgc gatcgcacgc ttcctgatac
     2221 gcctgataca ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc
     2281 ggacgcaggc cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac
     2341 tgcttctcgt cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttcg
     2401 ctatcgagga tctggaaatc ctgcggcaga ttggcgtcca tatggtgcgc acgcagcagg
     2461 cggtgcgcca gcccgtggaa ggtgcctacc cacatgccgc cctggctggt gcccatcagt
     2521 tgtccgatac gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc
     2581 gagtatggcg agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc
     2641 gtcttaccac tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct
     2701 tcgcgttgtt tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg
     2761 taaaaatagg ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca
     2821 gatttgctga ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgtggca
     2881 gtaaacggct gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct
     2941 gcattccgct gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga
     3001 tctcgccgat cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt
     3061 ttgagcgccc gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca
     3121 gttccggctg ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt
     3181 ttaaggtgtc gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa
     3241 tcattgctgc gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg
     3301 cttgttcaat cgaacgaaaa cgccaacgtg tcacgtcgtg ataaatctcg ggttccgctt
     3361 cccgtaccgc ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat
     3421 gataattttg cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat
     3481 cgtaaagggt atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa
     3541 aacgcattat ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg
     3601 ttgaaaatca agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagtagctc
     3661 ctgcacacca cgaagatcgc cgctcgactc cagcatatgc gtagcgaacg agtgacgtaa
     3721 tttatgtggg tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt
     3781 ctgcacatta cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc
     3841 gctaccaaac aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc
     3901 aatcggcagg cggcgctctt tgctgccttt ccccatcacc cacacttcgc cggactccag
     3961 gtcgaggtgt ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat
     4021 cacttccagc attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tatccagcag
     4081 ccgattcata tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga
     4141 aactcctttc gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct
     4201 acgtagcgca gaaagccgta acgccagact tgctgctccc agccctttac ggcgactgcg
     4261 tacagcaaaa ttgcgtacca tcgctgcatc gcattgttgc cagctttgca ggccgttttc
     4321 gctggcaaaa ttgatgatcg cctcaagctg acgctggtag ttaagtaggg ttatcgggct
     4381 aagctgacgc tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt
     4441 catacgcgtt caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt
     4501 aactgcgttc cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacaaca
     4561 cccaaatcag catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc
     4621 ggtagcacca ccagcagctc cggtccgtta agcggcccaa gatagtgctg ttcctgcccc
     4681 aaacgctgaa tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc
     4741 gacggcgcac ctaagcgcca gcgatctgga aacaggcgca gactcgcacc agctaggccg
     4801 agatcgcgcg cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg
     4861 agactgcgct gcaggtagag taggcgataa aacagccctt cgttggcgat agcctgctcc
     4921 atcaacaacg ccatgttctc ttccagaaca tgaatgtgat tacgtgcgcg ggccatgtgc
     4981 cactcgacca acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg
     5041 cgcgcattgc ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca
     5101 tcaagctccg tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc
     5161 catcgtagac atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg
     5221 cgatatcaag acgaccgccg gggagttcca cgcgtacttc ttcgtccagc aaaccttgct
     5281 gaatccctac cgccaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc
     5341 cacgctcata aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat
     5401 tggcgcgctc cggaaaacgc tcgtgacttt ccagaacagg accaagcgtt tctaccgccg
     5461 cggtatcgac atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc
     5521 ataagattgt ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa
     5581 acggcacggc ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat
     5641 cggtgacggt cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg
     5701 tcagtccttt cagacgcaca aaacgggcaa agcagcgcgc accgttaccg cactgcgcca
     5761 cttcactgcc atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg
     5821 gctcaaccac cagcagctgg tcaaacccta cccccaggtg ccgatccgcc aggcgacgaa
     5881 tcagctccgg tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc
     5941 caaggccatg cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg
     6001 acttttagta attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca
     6061 ccgtggattg cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag
     6121 gcgggaaata gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata
     6181 cagtgagtgc cttaaacacg tttttcatta tggattgcct gtaaagttca tcgatttctg
     6241 ctttctatca tcgcaggaga aggcgtaaaa gcaagcgttt agcgactctt tatgaagtct
     6301 gaaaagtgag gaaatgctgc gagtataacg cttactcgtg aggatttatg ttctgctggc
     6361 agtttcgtga tcaacacctc gcgccaggat ggctttatgc actatcgcaa tgtggtttgt
     6421 ttgttgtctt gttcattgtt tttatcgtcg gcatggggat gtcggctttc cgcacctgaa
     6481 cataatattt accagaaaca gggaaaaggc gtggtgtacc tgcgccctta taaagaaaca
     6541 aatctttctc tacctgacgt gaattacaag agtttgcgtc ggttacccaa tttattaata
     6601 gacccgacaa cactagacga atgggataaa gaaccgcctc tgactgattt gacaaccgac
     6661 tacctctatg agggcgcaca ggcctggtat cctcattatt catggcatag cgatggtcgc
     6721 aatatcctct acgcgggtga agttgtgcaa aaccctcccg gcacacctcc cgtcgatgtc
     6781 gcctcgttca aggcatgggg tgattttgcc gcagataaac acagtctcta ttttgaaggc
     6841 aagcgcaccg atgacaatgg tggtggaaat agcttggata tcaaaacgct gcatcaggtg
     6901 gaattccggc ctccctggga tcctgactta ctggggctga tattacgtga cgctaatttc
     6961 ttgtatatca atgggcatcg cctcgctgac cccgagagtt tccgtgtgct ggcgcagaag
     7021 tcctgggatc agcgaggaaa gttttccacc acgtttaacc cctgcattgc cgtaccattt
     7081 ggcccctggg ataccctggc tcgtacacgg accaaaatcc tgctcaacgg cgaacagctt
     7141 gatgccgacc cggacacctt ttccgtcgtg cgctggatgc ccggctcact cttgacctgg
     7201 cgtgataaaa acgggctgca gcgtaaagtc ctcgacaagg aaaatctggc gtgggatgaa
     7261 gatttaacaa agcactgtct ggatttttct ctgctggaaa agaaagtgtt ctggcgtaaa
     7321 ggacctgctt gtaaacagga agaattaccc ggactcgatc cggagcagtt tcaacccatc
     7381 agtgatgctg tcgcccagca tcaggactcg ctttatacca tcatcgaaac agagtctggt
     7441 aaccgtaagc tggagatcgt gaaacttgat gatcccaatc ttattatcaa caaacgtttc
     7501 aacgccggga aacgccacgg ctatttactt acgcgtgccg aagggtagcc ataccattcc
     7561 ggtttacacg tgtttgaatc tgacggaccg ctgatcttac tggataaccg ttctccggat
     7621 gaacgcgaag cccatcttaa tgaccatccc tttttgcgca gatggtatgc ccgcgataac
     7681 cgctacattt acagctttga tggcgcgcag ctctggcgat accgcaccgc tgatccgaaa
     7741 caagttcgct taatctggaa ggaacaacat tcgggatatg tctatggcgt aaattacaaa
     7801 acgggatatc tggacggaaa aattactgat gacggcgaat ttattcctgc cccgcgcaat
     7861 gaggcgacaa aatgaagggt caaataatca cagttgcact catcctgttg ggacacacat
     7921 ttctgacgcc tgctgtacag gccatagggt tcgactatta caatgatcac ggtgttatgt
     7981 cttacggtaa aggttattgg ggggataaga aaatcattgc ccagtttccg gagatgagca
     8041 gggccggttt gcgccttgtg aaaaatatct ctgggaaaac gggttatata ccttccgatg
     8101 aaataagcat agaaaatgag gaataccact gggtgacaga cggccgcgtt attctctggc
     8161 gtggcaaaat cgttagcaat ccaccgggaa cactcactgt cgatattgcc agctttcagg
     8221 ctatgggccg cttcgcggtc gataaatata gcctctattt tgacggacag cgcaccgaaa
     8281 gtaatagcgg cgcgtcacgt gttgagctgg cgacactaaa agctatcgaa ggtaactcta
     8341 ccacgctggt ggatagtaaa aatctctact tgtccggtcg acgtcagggg agcagcagtg
     8401 atgttactgt attagaaaaa agatggtggg gtattaatcc acgtcttatg agtgtaaaca
     8461 gaaattcata ttccaatgat ctgcttatcc gcagtgggca gaatatttat ttaaatggcg
     8521 ttcaccttac ggcgaatgca gactcatttg agataattcg ctggatacct cattcactgc
     8581 tggtttttcg cgacaataag ggtctgcatc gttatccctt tggtcaatta tcaggcaaag
     8641 cgatacccgt agatgatgac gtctcttttg aagtagggga aagtcgcgtt cgctggcgta
     8701 aacagctcac gcccgaccgt cagtggagca agtggataga cctaccaggt attgaacct
//
LOCUS       DADLTY010000171         8692 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.172, whole genome shotgun sequence.
ACCESSION   DADLTY010000171 DADLTY010000000
VERSION     DADLTY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.172"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..304
                     /locus_tag="JN334_003441"
     CDS             <1..304
                     /locus_tag="JN334_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA packaging protein"
                     /protein_id="HAY7439469.1"
                     /translation="LIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALS
                     STLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            276..2186
                     /locus_tag="JN334_003442"
     CDS             276..2186
                     /locus_tag="JN334_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HAY7439470.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            2187..2390
                     /locus_tag="JN334_003443"
     CDS             2187..2390
                     /locus_tag="JN334_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7439471.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2387..3980
                     /locus_tag="JN334_003444"
                     /pseudo
     CDS             2387..3980
                     /locus_tag="JN334_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3970..5475
                     /locus_tag="JN334_003445"
     CDS             3970..5475
                     /locus_tag="JN334_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HAY7439472.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5512..5859
                     /locus_tag="JN334_003446"
     CDS             5512..5859
                     /locus_tag="JN334_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HAY7439473.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5917..6945
                     /locus_tag="JN334_003447"
     CDS             5917..6945
                     /locus_tag="JN334_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HAY7439474.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6997..7383
                     /locus_tag="JN334_003448"
     CDS             6997..7383
                     /locus_tag="JN334_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HAY7439475.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7395..7772
                     /locus_tag="JN334_003449"
     CDS             7395..7772
                     /locus_tag="JN334_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7439476.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7759..8343
                     /locus_tag="JN334_003450"
     CDS             7759..8343
                     /locus_tag="JN334_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7439477.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            8340..>8692
                     /locus_tag="JN334_003451"
     CDS             8340..>8692
                     /locus_tag="JN334_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7439478.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMA"
BASE COUNT         2018 a         2051 c         2675 g         1948 t
ORIGIN      
        1 actcattgcc agatgggaac tgaccgcaga acaggctgtc gcacagcagt taaaaaatca
       61 ggtgtcaaaa ggaaatctta tcgataccgg attttgtatt tttgccctta gtaagctggc
      121 gatggcttta tccagtacgc ttgactccat tcctttgtcc atgcaacgac agttcccgga
      181 tttaacgccg cgtcatattg atcatctgaa aatccttatt gctaagggag cgaatcagtg
      241 cgcgcgtgcg ggggataaat taccggattt actcgatgaa tatatcagaa caacaactga
      301 gtaatatgat ggtggcagtc actatcgcac tgcagccact ggtcagggta ttgccggtga
      361 cggctgtaga atgggccgat caaaattatt acctgcctaa agaatcgtca tacggtgagg
      421 gggaatggaa aacgctgcag tttcaggtcg ccatcatgaa ctgcatgggc aatgacgaga
      481 ttcgtacagt taacctgatt aaatctgccc gtgttggtta tacaaaaatg gtgcttggtg
      541 tgatcgggta ttttattgag cacaaatccc gcaacactct tctttttcag ccgacggact
      601 cagctgctga agattttatg aagtctcacg tggaagcaac aattcgtgat gttccttgcc
      661 tgaaaaaact ttctccctgg ctgggccgga aacaccggga taatacgctc acactgaagc
      721 gtttttcctc tggtgtgggg ttctggtgtt tgggtggtgc tgctgccaaa aactaccgtg
      781 aaaaatctgt cgatgtggtc tgctatgacg aactctcctc gtttgaaccg gatgtggaaa
      841 aagaaggttc gccaacactg cttggcgata aacgtattga aggttcggta tggccaaaat
      901 ccatacgcgg ttcaacgcca aaaattaaag gctcctgtca gattgaaaaa gctgcgaatg
      961 aatctgcgca tttcatgcgg ttttatgtcc cttgccctca ttgcggggag gagcagtatc
     1021 tgaagtttgg cgatgatgcg acgtcgtttg gcctgaaatg ggagaagggt aaaccggaaa
     1081 cggtgtatta cctgtgtgaa cataatggct gcgtgatccg tcagtctgaa cttgatcaga
     1141 ccggggggcg ctggatttgt gacaataccg ggatgtggac gtgtgacggt ctgacgtttt
     1201 tcagtgcttc gggtaatgaa attccgccgc cgcgctccat cactttccat atatggacgg
     1261 cgtacagtcc gttcaccacc agggtacaga tagtctgtga ctggctggat gcactgaaag
     1321 atcccaacgg cgtgaaaact tttgtgaaca ccacgctggg cgagacctgg gaagaggccg
     1381 tgggcgaaaa actcgatcac caggtactga tggataaggt tgtgccttac acggcaacgg
     1441 tgcctgtccg ggtggtttat ctgacggcgg gcattgactc gcagcgaaac cgttttgaga
     1501 tgtatgtctg ggggtgggct ccgggagagg aagcctttct ggtggataaa atcatcatca
     1561 tgggacgtcc tgatgaggaa gagacgctgt tacgtgtgga tgcggcgatc aataaaaaat
     1621 accgccatgc agacggtacc gaaatgacca tttcccgtgt ctgctgggat atcgggggga
     1681 tcgatggcga aatcgtgtat cagaggtcaa aaaaacacgg tattttccgg gtactgccgg
     1741 taaaaggtgc ctctgtctat ggcaagccgg tgatcaccat gccgaaaacc cgcaatcagc
     1801 ggggtgtgta tctgtgtgag gtggggacgg ataccgcaaa agaaattctc tatgcccgaa
     1861 tgaaggccga tcccacccct gtgaatgagg ccacatcgta tgccatccgc tttcctgata
     1921 atccggagat tttttcgcag acagaggcac aacaactggt ggcggaggaa ctggtggaaa
     1981 agtgggaaaa aggaaagatg cgtctgttgt gggataacaa aaagcggcgt aacgaagcgc
     2041 tggactgcct ggtgtatgcc tatgcggcat tacgtgtgtc cgtgcaacgc tggcaacttg
     2101 atctggctgt gcagttgcat cccgcaagga tgcaactgca caaccgaccc ttgaagaact
     2161 ggcagcgaag ctgtccggag gagtgaatgg ttacagtcgc tgaactgcag gcgctgcgtc
     2221 aggcgcgcct tgatttatta accggtaaac gggttgtgtc ggtacagaaa gacggtcgca
     2281 ggatcgaata tacggcagcg tccctgaatg agcttaaccg tgcgatcaac gatgcggagt
     2341 cggttctggg tacgacccga cgccgccgac cgctgggagt gaggttatga aacgaacgcc
     2401 tgtcctgatt gatgtgaacg gcgttccgct tcgtgagagt ctcagctaca acggaggcgg
     2461 cgcaggattt ggcgggcaaa tggcagagtg gttaccaccg gcgcagagtg ccgatgcggc
     2521 cctgttgcct gcattgcgtc tggggaatgc ccgggcagat gatctggtgc gcaataacgg
     2581 tatcgcggct aatgcggtgg ccctgcataa ggatcatatc gtcgggcaca tgtttcttat
     2641 cagctaccgt ctgaactggc gttggctggg gatgcgggag accacggcaa aaagttttgt
     2701 cggggaggtg gaagcagcct ggtcggaata cgcagaagga atgtttggcg agatcgacgt
     2761 ggaagggaaa cgcacgttta cggaatttat ccgtgaaggg gtgggcgtgc atgcctttaa
     2821 cggcgaaatc tttgtgcagc cggtctggga tacggaaacc acgcagttat tccgtacgcg
     2881 ttttaaagcc gtgagtccga aacgggtgga cacgccagga cacggtatgg ggaaccactt
     2941 tctgcgggcc ggggttgagg tcgatcggta tgggcgggcg attgcgtacc atatctgtga
     3001 ggatgatttt ccgttctctg gtgccggacg ctgggagcgg atcccgcgtg agcttcccac
     3061 cgggcgtccg gccatgctgc atatttttga gccggtggag gacgggcaga cccgtggggc
     3121 taatcagttt tacagcgtaa tggagcggat gaagatgctg gactccctgc aggccacgca
     3181 gcttcagtcg gccattgtga aggcgatgta tgcagccaca atcgaaagtg aacttgatac
     3241 cgaaaaggca tttgaatata tcgcgggggc accgcaaggt cagcaggata atccgcttat
     3301 taatattctg gagaagttca cccgctggta tgacacgaat aacgtgacgc tgggcggtgt
     3361 taaaattccg cacctttttt cctggcgatg cgctgaacct gcagacagcg caggattcag
     3421 acaatggatt ttcggcgctt gaacaggcgc tgctgcggta tattgcagcc ggtcttggcg
     3481 tttcctacga acagttgtcc cgtgattact cgaaggtcag ttactcaagt gcccgcgcct
     3541 ccgccaatga gtcgtggcgc tattttatgg ggcggcgaaa atttattgcg gcccggctgg
     3601 ccacgcagat gttttcctgc tggctggaag aggcgcttct tcgggggatt attcgtccgc
     3661 cacgggcgcg ttttgatttt taccaggcgc gttcagcctg gtcacgggca gagtggatta
     3721 gctccggaag aatggccatt gacgggctca aggaggtcca ggaatcggtg atgcgcattg
     3781 aggccggact gagcacgtat gagaaagaac tggcactgat gggcgaagat tatcaggaca
     3841 ttttccgcca gcaggtcagg gaatccgcag agcgcgaaaa agccggactc tcacgtccgg
     3901 tgtggatagc ccaggcgtat cagcagcaga tagcggagag tcgcaggccg gaagaggaga
     3961 caacatcacg tgagacgtaa tctttcacat attatcgctg cggcattcaa tgagccgctg
     4021 cttctggagc ccgcctatgc gcgggttttc ttttgcgcgc tcgggcgtga gatgggcgca
     4081 gcaagccttt cggtaccgca acagcaggta cagcttgacg ctcccgggat gctggctgaa
     4141 acggacgagt acatggccgg aggcaaacga cctgcccgtg tttaccgggt ggtgaacggt
     4201 attgctgtac tgccggtgac cggcacgctg gtacaccggc tgggtggcat gcggccattt
     4261 tccggaatga ctggctatga cggcattgtc gcctgtcttc agcaggcaat ggcggacact
     4321 caggtacggg gcgtactgct ggacattgac agttctggcg ggcaggccac cggcgcgttt
     4381 gactgcgctg acatgattta ccgtctccgg cagctgaagc ctgtctgggc actgtgcaat
     4441 gacacggcct gttcagccgc catgttactg gcatcggcct gctcccgacg gctggttacc
     4501 cagacatccc gtatcggctc cattggggtg atgatgggcc atgtcagcta tgccggtcat
     4561 ctggcacagg ccggtgtgga tatcacgctg atttacgccg gatcgcacaa ggtggatggc
     4621 aatcagtttg aatccctgcc agcaaaggtt cgccaggaca tgcagcagcg cattgatgcg
     4681 gcgcgccgga tgtttgctga aaaagtggcg atgtataccg gtttatctgt ggatgcggtt
     4741 atgggaacag aggccgccgt ttttgaaggt cagtccggta ttgaggccga actggcggat
     4801 gaattaatca atgcgtcgga cgccatcagc gtgatggctg cgacgctgaa cacacacgat
     4861 acaggaggca ctatgccgca attaactgca acggaggctg ccgcgcagga gaaccagcga
     4921 gtgatgggga tcctgacgtg tcaggaagcg aaaggacgtg aacaactggc cacgatgctg
     4981 gcagggcaac agggtatgag cgttgagcag gcccgggcga ttctggccgt ggcggcaccg
     5041 caacaatctg gtgtatctgc gcagagtgaa gccgatcgca ttatggcatg tgaagaggcg
     5101 aaaggacgtg aacaactggc ggcaacgctg gcggcgatgc cggagatgac ggtggaaaaa
     5161 gcccgtccga ttctggctgc ttcaccgcag gcgaatgccg gaccctcact tcgtgatcag
     5221 attatggccc tggatgaggc aaaaggggca gaggcgcagg ctgaaaaact ggcggcctgt
     5281 ccggggatga ccgtggagaa tgcccgggct gtactggcgg cagcctcagg cagggcagaa
     5341 ccggtttctg catccacaac cgccatgttt gaacatttca tggcgaacca ttcaccggca
     5401 gcggtacagg gtggcgtgcc acagacgtca gcagacggtg atgcggacgt gaaaatgctc
     5461 atggccatgc catgaagtca gtgctgacca tcaatatgag gtttttacaa tatggtaacg
     5521 aaaaccatca ctgaacagcg tgcggaagta cgtatttttg ccggtaatga tccggcccat
     5581 accgccacag gctgcagcgg gatttcctcg gcaacaccgg ctctgacgcc cctgatgctg
     5641 gatgaggata ccgggaaact ggtggtctgg gacggacaga aggcaggcca tgtggttggc
     5701 atactggtac tgccgcttga aggtacagag acggtgctga cgtattacaa gtcggggacc
     5761 tttgcgacgg aggccatcca ctggcctgag agagtggata cacacaaaaa ggcgaatgcc
     5821 tttgccggca gtgccctgag tcacgcagcg ctgccgtaac attttatcag gtcactgcgg
     5881 tagcctgact gatttctgaa tgaaaggaac tgatttatgg gattgtttac gacccgccag
     5941 ttactcggtt ataccgaaca aaaagttaaa tttcgtgcgc tgtttctgga gctgtttttc
     6001 cgccgtacgg tgaatttcca taccgaagag gtaatgctgg acaaaattac cggaaaaacg
     6061 ccggtggcgg cctatgtctc cccggttgtt gaaggaaagg tgttgcgtca ccgtggcggt
     6121 gaaactcgtg tactgcgccc gggctacgtt aagccgaaac acgaatttaa ttaccagcag
     6181 gcggtggagc gtcttcccgg tgaagatccg gcccagctga acggcccggc ttaccgtcgc
     6241 ctgcgtatca tcaccgataa cctcaaacag gaagagcacg ccattgtcca ggtggaagag
     6301 atgcaggcgg tgaatgccgt gctgtatggc aaatacacca tggaaggaga gcagtttgaa
     6361 actgttgagg tcgattttgg ccggtcggtg ggaaataaca ttacgcaggc aggagggacg
     6421 gagtggagcg ctcaggaccg cgataccttt gatccgacgc atgatctgga tgcgtactgt
     6481 gattttgctt ccggcaccat caatattgcc attatggatg gtaaggtctg gcgtctgctg
     6541 aatggcttta agctgttccg cgaaaaactg gatacccgtc gtggctcaaa ttcacagctg
     6601 gaaacggcag tgaaagacct gggggcggtg gtgtccttca aagggtatta tggtgatctg
     6661 gcaatcgtgg tggcaaaaac gtcctatgtg gcagaggacg gtaccgaaaa acgttatctg
     6721 ccggacggca cactggtact gggaaatacg gccgcagagg gtattcgttg ctacggtgcc
     6781 attcaggatg cgcaggcatt gtctgaaggc gtggtggcgt cttcccgtta tccgaaacac
     6841 tggctgaccg tgggtgatcc ggcgcgtgaa ttcaccatga cgcagtccgc gccgctgatg
     6901 gtgctgccgg atccggatga gtttgtggtg gtgcaggtga aataatccgt gagcgggagc
     6961 taaatgctcc cgtttttcat tcaatcagga gcgggtatgg ctacgaaaga agaaaacctg
     7021 aaacgtcttt gtgagctggc tgagaggctg gggcgtgaac cggatgtctc cggtagtgcg
     7081 gcagatattg cgcagcgagt ggcggaactg gaggaagagc ttggagatgc gggggaggtc
     7141 gcagagccgg atacgtactc accatcgaaa gatgatccga ccgcgcataa aggcgagtca
     7201 gttcctgaac agccaggcat tgttaccgga gacgaaacgg ggcaggttac ggtggtggca
     7261 ctggcgacac tgcatacaaa atgtaatggt tcagtgatat ttgtttcacc gggaacgtcg
     7321 tttcgtgttt ctgctgaggt ggctgccagc atggcggcag gtggtctggc aaaaaggcag
     7381 taacaggaga tgtcgtggac ggtttcgata atctgtttga tgctgcgctt gccggtgtca
     7441 atgaagtcat tctccgtgat atggggatca gcgccgtcat tacgtccgga gaacttgagg
     7501 ggacacacct gacaggggtt tttgatgatc ccgaaagtat ttcttttgtt gccgggggga
     7561 tccggcttga ggattcctca ccgtgcctgt ttgtgaaaac ggcagatata tcgcagttac
     7621 gccgtcagga tacgttaact atcggggatg attctttctt tgtggatcgt attactccgg
     7681 atgacggggg atgctgttat atccggttaa ggcggggcag tcctgcccca cagaataatg
     7741 ccagaatgag gcgatatgat gaagggactt gagaatgcca tccgcaatct gaacagcctc
     7801 gatacccgca tggtgccaca gaccagcgca tgggcggtaa accgtgtggc ggcaaagatt
     7861 gtttctgtcg ccacacggca ggttgcgcag aataccgttg ccggggataa ccaggtgaaa
     7921 ggtattcccc tgaaaacggt cagggagcgt gtcaggctgc ttaaagccag cccctcagga
     7981 aaaatgtatg cccgcatgcg tgttaaccgg ggtaacctgc ccgccattaa gctgggtacc
     8041 gcacaggtca gactggcccg ctcccggcat ggcagcaact cacgtcatcg tggcagcgta
     8101 ctgaaagtgg ggaaatacct tttccgggat gcgtttattc agcaactggc gaatggtcgc
     8161 tggcatgtga tgcggcgtat tgacggcaaa aatcgttacc ccattgatgt ggtgaaaatc
     8221 ccgatgtccg gaccgctgac acaggcattt gaagatgccc gcgaccgcat cattgctgcg
     8281 gaaatgccga aacagctggg gtatgccctg aaacaacaac tgaggttaca tctgagtaaa
     8341 tgaaccgaca cacacaaatc cgtcagagcg tgctggcacg ccttcgggaa cagtgtggag
     8401 acagcgccac gttttttgac gggcttccgg catttattga tgcgcaggaa ctgcccgccg
     8461 tggcggtgtg gctgagtgat gcgcagtaca ccggaaaaat gacggatgag gacgactggc
     8521 aggctgttct gcatattgcc gttttcgtcc gtgcacaggc gccggattct gagctggata
     8581 cgtggatgga gaacaccata tttccggcca tgaaggatat tccggtgctt tccggactca
     8641 tcgacaccat gatcccactc ggttttaact atcaacgtga taatgagatg gc
//
LOCUS       DADLTY010000172         8684 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.173, whole genome shotgun sequence.
ACCESSION   DADLTY010000172 DADLTY010000000
VERSION     DADLTY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.173"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(119..739)
                     /gene="entD"
                     /locus_tag="JN334_003452"
     CDS             complement(119..739)
                     /gene="entD"
                     /locus_tag="JN334_003452"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HAY7439479.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(914..3154)
                     /gene="fepA"
                     /locus_tag="JN334_003453"
     CDS             complement(914..3154)
                     /gene="fepA"
                     /locus_tag="JN334_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HAY7439480.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            3397..4599
                     /gene="fes"
                     /locus_tag="JN334_003454"
     CDS             3397..4599
                     /gene="fes"
                     /locus_tag="JN334_003454"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HAY7439481.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            4602..4820
                     /locus_tag="JN334_003455"
     CDS             4602..4820
                     /locus_tag="JN334_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HAY7439482.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            4817..8662
                     /gene="entF"
                     /locus_tag="JN334_003456"
     CDS             4817..8662
                     /gene="entF"
                     /locus_tag="JN334_003456"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HAY7439483.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         1874 a         2323 c         2395 g         2092 t
ORIGIN      
        1 ttgtgcgggg agtaatcccc gcatatccgg ttgtcaggtc agagtggtaa ggcacctgct
       61 ttacactttc gcccgcagtc agtgatggct gcgggcgaat cgtaccagat gttgtcaatt
      121 aatcgtgttg gcacagagtt atgactattt tttcttttat ctgccagtgc acagcaaaca
      181 tctcattctc acgatgaatg atgacctgct gtttattcca gctaattatc tgatagtcca
      241 gaaaacctgc atcagtttgg atctcacttg ccttaaatgc gctctctttg gcggaaaatg
      301 caagtgtcag cgccagagaa aaggctaaac cgcagtctgc gagtcgctcg tgttctgctg
      361 gtgtaataat gttgtctgtc aattcggtcg ctgtttgcgc agaaaaaatt tcttcgatat
      421 caacgccaat cggttgacgg gaaaccacgg ccaatgccgt agccccgcag tggctaatac
      481 tgccgtatac ctccgcaggc cacaccggtt ggcgtagctc gccgattgcg ggcacacatt
      541 tatagccata ttcccgcaag gcataaacag cagcgatccg tccggctaaa tgctctgttt
      601 tacgtttacg tccagcgtgt tgcagttgtg cgtagtgcgg cagccagagt aaatcctgct
      661 cacaaaaatt cgccggatcg aactcaacaa aatgcagcgt atgtccggca aaggggaggg
      721 aggtatgcgt agttttcata tcgaccatat tcgagactga tgacaaacgc aaaactgcct
      781 gatgcgctac gcttatcagg cctacgtgat cctgcaatat attgaattgg caagatcttt
      841 gtaggccgga taaggcgttc acgccgcatc cggcgtgaac gacgcgcact ttgtcaacaa
      901 tctgacgtta gcatcagaag tgagtattaa tgctcatata ccacgtacgt cccggctcgt
      961 tataggtata cgcgccggca ccggcgatat agttggctcc tgccagatcg cccgtggtct
     1021 gggcattacc tgcacgccac aaacgtttgt cgaacaggtt gtccacgccg ccggtcagac
     1081 tgacattctt cgtcacatcc caggtcgcgc tcagacccac aatgctgtaa ggactgattt
     1141 ctttggtttc cgggccaacc gctggctgac ctttatagtt gtacttcttc ggctgctgct
     1201 tgccgtacca ggtgaaggtc gtttgcatcg acaaatcttc ccgtgcctgc cagctcagcg
     1261 ttgagttcaa cgtgtactcc gggatgatcg acaaacggtc gcccgtggtt ttgttttcac
     1321 tcttcagcat ataagtgatg ttattggtcc acatcaccgt ttcgctaact ggtacgttta
     1381 acgatccttc cagaccttca accactgctt tcggcacgtt atcccactga tagagatcag
     1441 tgccgactgc gttttgccct acagccacat agcccgcttc aatcttattg cgataatcgt
     1501 tacggaacca ggtgacgccc gccagccagc cgtcgcgttt gaactccaga ccaatctctt
     1561 tgttgatgct ggtttctact ttcaggtcat cgttaccttg cagatagcag ccgcccgcgc
     1621 tggcatagca gccctgacct ttactgtaga gaatgtagtt cgggttagtc tggtataggc
     1681 tcggcgcttt ataggcgcgg gcaatgccta ttttcagcgt gaagtcatcg cctaaacctt
     1741 gcgatatgtt cagcgccggg ctccagttat tgccgacaat actgtgatga tcgaaacgca
     1801 gccccggcgt tacgatggtg ctgtcagtca gctccatgtt gttttcggca aacagcgaga
     1861 aaatttctgc ttttgaatac gggctacggt cggtggcact cacgccatca atagcgccac
     1921 cggtattcgt tccggtcagt gcttgggtgt tggaacttaa gtccttcatc cgttgctgat
     1981 tccactccgt acccagcgtc agcgtctggt taacgaggaa atcaatcggc aggttaactt
     2041 cgctgtgcag catcacgtca tcgagatcga tatcgacgaa atcctgtgtc gctttttcgt
     2101 taaatttccc ttcggtaccg cccgccagac cttccggaat acgcgagtta cgggtgtgtt
     2161 catactgcac ccagttgctg gtggtcacgc cgttatccca gccaccgttc caggtcagcg
     2221 cgtagttctg gcgatacagg cggttggttt catcgccata tttcgagcga gtgtaagcgt
     2281 cagagttggt gttctgggta tcgcccgcat acaggttacc ctggcggctg taacccgctt
     2341 ccagttccag cgattgcaat ggtgcgaagt cccagcgcac cacgccgtta atatctttat
     2401 tgatcacccc ttcgcgcccg gctggtaacg tcgtggcata cgttccggca cgcgcggact
     2461 gatggccctg gttgatatcc cacgcgtcag cctgagtttt gtcgaggttg ccatacaagc
     2521 ggaagctgaa ttcgtcgccc agcggaccgg tcaggctaaa gttagtgcgt ttggtggcac
     2581 cttcctcttt atgttctggc gcattgaaat atgcgtccca ggagccgtgc cactcgccgc
     2641 tgcctttttt ggtaatgatg ttaaccacgc cgcccgccgc gccgttgcca taacgcgcag
     2701 ctgccggacc acgcagaact tcaatacgtt caatcatttc aggtggcacc caggaggtat
     2761 caccacgggt atcgcgctcg ccacgccagc cctgacgcac cgagttacgg ctgcttaccg
     2821 gcttgccgtc aatcaaaatc agcgtgtttt ccggacccat accgcgaata tcaatctgtc
     2881 ggttattccc acgctgacca ctggtggagt taccggtcag gttaacgcct ggcatggtac
     2941 ggatgatctc cgacacatcg cgggcaaccg ggtttttgcg gatttcatct gcggtgatgg
     3001 tcgaaacgcc aggagcctgc aagttctgct cggcggcggt aacgacaatg gtatcgtcat
     3061 gtgaaacagg tgtatcggtc ggctcttgtg cctgcgctac cccataaatc cccagattga
     3121 ccaacaaggc cagggaatga atcttcttgt tcattgtttt attcctgcat ttttgccacg
     3181 aattgcaact gtcgggcatg gtcgtcatca acacgacgca tcccgttacc gcgaaaacct
     3241 ttgatcctga aagacacgca gtgcagttgg ttaattaatg tccgcgcttc ccacggcgcg
     3301 ccattacgct attgcaaatg caaatagtta ttaataacat tatcaatata tttctgcaat
     3361 caatgaaaaa ttgcacagta aacatggggt tatggtgtga cggcgttaaa agtaggaagt
     3421 gagagctggt ggcagtcgaa acatggcccg gaatggcagc gtctgaatga cgaaatgttt
     3481 gaggtcactt tctggtggcg tgatccccaa ggttctgaag aatactcgac gataaagcgc
     3541 gtatgggtct acatcactgg tgtgaccgat catcatcaga acagccagcc ccagtcgatg
     3601 cagcgaattg caggtactga tgtctggcag tggacgacac aactcaatgc caactggcgc
     3661 ggcagctact gctttattcc caccgaacgc gatgacattt tttctgcacc atcccccgat
     3721 cgcctcgaat tacgtgaagg ctggcgaaaa ctattacccc aggcaatagc cgatccgctg
     3781 aatccacaaa gctggaaagg cgggctaggg cacgctgttt ctgcactcga aatgccgcaa
     3841 gcgcctctgc aaccgggatg ggattgtccg caagcgccag aaataccagc caaagaaatt
     3901 atctggaaaa gtgaacggtt gaaaaattca cgtcgtgtat ggatttttac caccggcgat
     3961 gtaacagcag aagaacgccc gctggcagtt ttgctcgatg gcgaattttg ggcgcaaagt
     4021 atgcccgtct ggcctgtgct gacttcgctg acccatcgtc agcaacttcc tcccgccgtg
     4081 tatgtgttga tcgacgctat cgacaccacg caccgcgccc acgaactgcc gtgtaatgcg
     4141 gatttctggc tggcagtaca gcaagagtta ttacccctgg tgaaagttat tgcccctttt
     4201 agcgatcgcg ccgatcgcac cgtggtcgcc gggcagagtt ttggtgggct gtccgcgctg
     4261 tatgccggac tgcactggcc tgaacgcttt ggctgtgtat taagccagtc aggatcgtac
     4321 tggtggccgc atcggggcgg gcagcaagag ggcgtgttac ttgaaaagct aaaagctggt
     4381 gaagttagcg ccgaaggtct gcgcattgtg ctggaagcgg gtattcgcga gccgatgatc
     4441 atgcgggcca atcaggcgct gtatgcgcaa ttacacccta taaaagaatc cattttctgg
     4501 cgtcaggttg acggcggaca tgatgcgctt tgttggcgcg gtggcttgat gcaggggtta
     4561 atcgacctct ggcaaccact tttccatgac aggagttgaa tatggcattc agtaatccct
     4621 tcgatgatcc acagggagcg ttttacattt tgcgcaatgc gcaggggcaa ttcagtctgt
     4681 ggccgcaaca atgcgcctta ccggcaggct gggacattgt gtgtcagccg cagtcacaag
     4741 agtcctgcca gcagtggctt gaagcccact ggcgtactct gacaccggcg aatttcaccc
     4801 agttgcagga ggcacaatga gccagcattt acctttggtc gccgcacagc ccggcatctg
     4861 gatggcagaa aaactgtcgg atttaccctc cgcctggagc gtggcgcatt acgttgagtt
     4921 aaccggagag gttgatgcgc cattactggc ccgcgcggtg gttgccggat tagcgcaagc
     4981 agatactttg cggatgcgtt ttacggaaga taacggcgaa gtctggcaat gggtcgatga
     5041 tgcgctgata ttcgaactgc cagaaattat cgacctgcga accaatattg atccgcacgg
     5101 tactgcgcag gcattaatgc tggcggattt gcaacaagat ttgcgcgtcg atagcggtaa
     5161 accactggtc tttcatcaac tgatacaggt ggcagataac cgctggtact ggtatcagcg
     5221 ttatcaccat ttactggtcg atggcttcag ttttccggcc attacccgcc agatcgccaa
     5281 catttactgc gcattgctgc gtggcgaacc aacgcctgct tcgccgttta cgcctttcgc
     5341 tgatgtagtg gaagagtacc agcaataccg cgaaagcgaa gcctggcagc gtgatgcggc
     5401 attctgggtg gaacagcgtc gccaactgcc accgcccgcg tcactttctc cggcaccttt
     5461 agcggggcgc agcgcctcgg cagatatttt gcgtctgaaa atggaattta ccgacgggga
     5521 attccgccag ctggctacgc aactttcagg tgtgcagcgt accgatttag cccttgcgct
     5581 gacggctttg tggctggggc gattgtgcaa tcgcatggac tacgccgccg gatttatctt
     5641 tatgcgtcga ctgggctcgg cggcgctgac ggctaccgga cccgtgctca acgttttgcc
     5701 gctgggtatt catattgcgg cgcaagaaac gctgccggaa ctggcaaccc gactggcaac
     5761 ccgactggca gcacaactga aaaaaatgcg tcgtcatcaa cgttacgatg ccgaacaaat
     5821 tgtccgtgac agcgggcgag cggcaggtga tgaaccgctg tttggtccgg tactcaatat
     5881 taaggtattt gattaccaac tggatattcc tggtgttcag gcgcaaaccc ataccctggc
     5941 aaccggtccg gttaatgacc ttgaactggc cctgttcccg gatgaacacg gtgatttgag
     6001 tattgagatc ctcgccaata aacagcgtta cgatgagcca acgttaatcc agcatgctga
     6061 acgcctgaaa atgctgattg cccagttcgc cgcggatccg gcgctgttgt gcggcgatgt
     6121 cgatattatg ctgccaggtg agtatgcgca gctggcgcag atcaacgcca ctcaggttga
     6181 gattccagaa accacgctga gcgcgctggt ggcagaacaa gcggcaaaaa caccggatgc
     6241 tccggcgctg gcagatgcgc gttacctgtt cagctatcgg gaaatgcacg agcaggtggt
     6301 ggcgctggcg aatctgctgc gtgagcgcgg cgttaaacca ggggacagcg tggcggtggc
     6361 actaccgcgc tcggtctttt tgaccctggc actccatgca atagttgaag ctggcgcggc
     6421 ctggctaccg ctggataccg gctatccgga cgatcgcctg aaaatgatgc tggaagatgc
     6481 gcgtccgtcg ctgttaatta ccaccgacga tcaactgccg cgctttagcg atgttcccaa
     6541 tttaaccaac ctttgctata acgccccgct taccccccag ggcagtgcgc cgctgcaact
     6601 ttcacaaccg caccacacgg cttatatcat ctttacctct ggctccaccg gcaggccgaa
     6661 aggggtaatg gtcgggcaga cggctatcgt caaccgcctg ttgtggattc aaaatcatta
     6721 tccgctcaca ggcgaagatg tcgttgccca aaaaacgccg tgcagttttg atgtctcggt
     6781 gtgggagttt ttctggccgt ttattgccgg ggctaaactg gtgatggctg aaccggaagc
     6841 gcaccgcgac ccgctcgcta tgcagcaatt ctttgccgaa tatggcgtaa cgaccacgca
     6901 ctttgtgccg tcgatgctgg cagcatttgt tgcctcgctg actccgcaaa ccgctcgcca
     6961 gagttgcgtg acgttgaaac aggttttctg tagtggtgag gccttaccgg ctgatttatg
     7021 ccgcgaatgg caacagttaa ccggcgcgcc gttgcataac ctgtatggcc cgacggaagc
     7081 ggcggtagat gtcagctggt atccggcttt tggcgaggaa ctggcacagg tgcgcggcag
     7141 cagtgtgccg attggttatc cggtatggaa tacgggtctg cgtattcttg atgcgatgat
     7201 gcatccggtg ccgccgggtg tggcgggtga tctctatctc accggtattc aactggcgca
     7261 ggggtatctt ggacgacccg atctgaccgc cagccgcttt attgccgatc cttttgctcc
     7321 tggtgaacgg atgtatcgta ccggagacgt tgcccgctgg ctggataacg gcgcggtgga
     7381 gtacctcggg cgcagtgacg atcagctaaa aattcgcggg cagcgtatcg aactgggcga
     7441 aatcgatcgc gtgatgcagg cgctgccgga tgtcaaacaa gccgttaccc acgcctgtgt
     7501 gattaaccag gcggcagcca ctggtggtga tgcgcgtcag ttggtgggct atctggtatc
     7561 gcaatcgggc ctgccgttgg ataccagcgc attgcaggcg cagcttcgcg aaacattgcc
     7621 gccgcatatg gtgccagtcg ttctgctgca actgccgcag ttgccactta gcgccaacgg
     7681 caagctggat cgcaaagcct taccgttgcc tgaactgaag gcacaagcgc cagggcgtgc
     7741 gccgaaagcg ggcagtgaaa cgattatcgc cgcggcattc gcgtcgttgc tgggttgtga
     7801 cgtgcaggat gccgatgctg atttctttgc gctgggcggt cattcgctac tggcaatgaa
     7861 actggcagcg cagttaagtc gacagtttgc tcgtcaggtg acgccggggc aggtgatggt
     7921 cgcgtcaacc gtcgccaaac tggcaacgat tattgatggt gaagaggaca gctcccggcg
     7981 catgggattc gaaaccattc tgccgttgcg tgaaggtaat ggcccgacgc tgttttgttt
     8041 ccatccggcg tccggttttg cctggcagtt tagcgtgctc tcgcgttatc tcgatccaca
     8101 atggtcgatt attggcattc agtcgccgcg ccctcatgga cccatgcaga cagcgacaaa
     8161 cctggatgaa gtctgcgaag cgcatctggc aacgctactt gagcaacaac cgcacggcat
     8221 tgcggcgcgg ctgcgtgccc gtggcgaaca ggtggcattt cttggtttgc tggatacctg
     8281 gccgccagag acgcaaaact ggcaggaaaa agaggctaat ggtctggacc cggaagtgct
     8341 ggcggagatt aaccgcgaac gcgaggcctt cctggcggca cagcagggaa gtacttcaac
     8401 ggagttgttt accaccattg aaggcaacta cgctgatgct gtgcgcctgc tgacgactgc
     8461 tcatagcgta ccgtttgatg gtaaagcgac gctgtttgtt gctgaacgca cgcttcagga
     8521 aggtatgagc cccgaacgcg cctggtcgcc gtggattgcg gagctggata tctatcgtca
     8581 ggattgtgcg catgtggata ttatctctcc aggggcgttt gtaaaaattg ggccgattat
     8641 tcgcgcaacg ctaaacaggt aaattaatat tatctataaa ccca
//
LOCUS       DADLTY010000173         8630 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.174, whole genome shotgun sequence.
ACCESSION   DADLTY010000173 DADLTY010000000
VERSION     DADLTY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.174"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(770..1966)
                     /gene="hemY"
                     /locus_tag="JN334_003457"
     CDS             complement(770..1966)
                     /gene="hemY"
                     /locus_tag="JN334_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HAY7439484.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1969..3168)
                     /gene="hemX"
                     /locus_tag="JN334_003458"
     CDS             complement(1969..3168)
                     /gene="hemX"
                     /locus_tag="JN334_003458"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY7439485.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQGDTP
                     AAAPQGE"
     gene            complement(3190..3930)
                     /gene="hemD"
                     /locus_tag="JN334_003459"
     CDS             complement(3190..3930)
                     /gene="hemD"
                     /locus_tag="JN334_003459"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAY7439486.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3927..4868)
                     /gene="hemC"
                     /locus_tag="JN334_003460"
     CDS             complement(3927..4868)
                     /gene="hemC"
                     /locus_tag="JN334_003460"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAY7439487.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5255..7801
                     /gene="cyaA"
                     /locus_tag="JN334_003461"
     CDS             5255..7801
                     /gene="cyaA"
                     /locus_tag="JN334_003461"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HAY7439488.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7841..8161)
                     /gene="cyaY"
                     /locus_tag="JN334_003462"
     CDS             complement(7841..8161)
                     /gene="cyaY"
                     /locus_tag="JN334_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HAY7439489.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8209..>8630)
                     /locus_tag="JN334_003463"
     CDS             complement(8209..>8630)
                     /locus_tag="JN334_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HAY7439490.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1965 a         2232 c         2217 g         2216 t
ORIGIN      
        1 cagttgacgg ggtatctgct gcgctggcca tgagaggaag agcggcggcg acagcaacaa
       61 caagacgttt gggtgaaaac gaaaattcca tgcaaaatgc tccggtttca tgtcatcaaa
      121 atgatgacgt aattaagcat tgataattga gatccctctc cctgacagga tgattacata
      181 aataatagtg acaaaaataa attatttatt tatccagaaa atgaattgga aaatcaggag
      241 agcgttttca atcctacctc tggcgcagtt gatatgtaag gcaggtttat tatatcgcgt
      301 tgattattga tgctgttttt agttttaacg gcaattaata tatgtgttat taattgaatg
      361 aattttatca ttcataataa gtatgtgtag gatcaagctc aggttaaata ttcactcagg
      421 aagttattac tcaggaagca aagaggatta cagaattatc tcataacaag tgttaaggga
      481 tgttatttcc cgattctctg tggcataata aacgagtaga tgctcattcc atctcttatg
      541 ttcgccttag tgcctcataa actccggaat gacgcagagc cgtttacggt gcttatcgtc
      601 cactgacaga tgtcgcttat gcctcatcag acaccatgga cacaacgttg agtgaagcac
      661 ccacttgttg tcatacagac ctgttttaac gcctgctccg taataagagc aggcgttttt
      721 ttatgcatca gaaaggtccc ggaggtgctt tcctccgggt gagaaggaac tactgtggcg
      781 ggttattctg caacgttaac atcaaaccgt cgcgacgcat cgctgcagct tcttccggct
      841 tgtgcagtct gtccagcgcg tcggcaagcc atgcgtaatc gtaggcgtcc ggacgttgtt
      901 tcagcgccgc gcggaaggcg agcgatgctt cctgccattc accgtgcttc atcagtgact
      961 gacccaatgt gctccacaac agcgggcgat caccgacgtt tttgatttgc tggcgtagca
     1021 ctttttccag ctgttccggg ttattggttt tcagacgcgg gatcggcagc agtaggcgat
     1081 cgtcatactg gcgtttcagg ccgtcgatga taatttgctg ggcagtatca tgatcgtcac
     1141 attcaataag atgttccgcc attgccacct gcaacgctac ctgatgacgc gttttccggc
     1201 tttggttttt ccaccagtta cgcaaacctt cactaccgtt atcggcacgc gcctgatcca
     1261 tcaggccaat ccatgcctgt tgttccagca ttgcacgatg ttcttcatca ccaacatggg
     1321 ctttcgccat tgatgggatg atatccagca gagaactcca tgcccctgtg cggatatacg
     1381 cctgttccgc cagacgtaat acttcaggat ggcgtggcgt aacttccagc agcttatcca
     1441 cgccgtggcg tgcagcatgg ttttcattac gggccagttg cagacgtaca cgggtgattt
     1501 ctaccggaat ggtgtcattg ccggccagct ccgctgcgcg ttccagatgt tggttggcgc
     1561 gtgcttcatc accacgttgt tgcgcggctt cggcagccag tagatagttc accaccggtt
     1621 gttcagcgtg atcggcattt ttcgccatca gcttttcaac ttgctgataa tcaccttccg
     1681 ccagtttcag cagcgcctgt tcggtctgct tacgcgcacg gcgacgctta cgtccgacaa
     1741 accatccacg ggtgtgcgcg ccagtgcgga agatccgccg cagtagccac tcaatggcaa
     1801 acagcactac catcgccaga atcaatatga tcgccaggcc cgtgacgctg gtttcgatat
     1861 tgtagttgtc ggtctggatc agcacataac cctgatgacc ggcaatcatc gggccaacca
     1921 ctatccccgc aatcagcaaa acaaagagta ataacacttt tagcatggtt attctccttg
     1981 cggcgcggct gccggagtat caccttgcgg tgcaggtgca ggtgcaggtg caggtgcagg
     2041 tttagcttcc gttgtccccg ctgccggttg tgccagcagg ttacgcacgc gagtctgcat
     2101 cagtttttcc agcatcgcct ggctttgcag ggtttccgga agatccatcg agatattttg
     2161 ctggcttaac tggtccacct cgtcgaggaa cgctttggtg gtggcatcat cagtatcgta
     2221 gtaagcacgt acccaggtgg agacgttctc cagcgcctgg cgataagtct cttcctgatg
     2281 acgcggtaca gcttgtgctg cgaccagcag gcgagagcga atattttcgc gcagatagat
     2341 atcctgattt ggcgctaaca gcggtacggc ggtgtcatca cgacggcgaa tcgtaatgaa
     2401 gttgtccata aagttctgcc agcttttttg cagattgata cgccattcgc tgatggaact
     2461 ggaaagctct tcaccgtctg aatccatcgg cgaaccatcg ctgtcattat cggccagacg
     2521 caggttatct acctgatttg aaagctgatt aagcttaagg atgatgccgt cataatccac
     2581 ctgcgatact gcagaaaggc tggcgatatc atcggtaatt gcccgacgaa cggtaatcag
     2641 actcgggtca ttcatatccg ccaggctggc gtctgcactt ttcagcaacg ctgcagaggt
     2701 cgtgacgtcc tgatcgctcc acagcttccg tccggcgagt ttcaccagaa aatcggcctg
     2761 agccagcagc caggttttag catcgctgcc ggaaatggtg gcgatctttt gttggacttc
     2821 atccaactgt tttgccagcg tttcttgctg acgattcgcc tgcttaagct gcgcagcttg
     2881 ttgcttaata atgccttcca gctcggcttt ttggctctcc tgggcttttt gcaatgccgt
     2941 cagttggtta gccagggcat cgctggtggc ggtctgattg acggcctgtt gtttacccca
     3001 gccatacaaa ccgacacccg ccgccagagc aatagcgata gccaccgcgc tgagaatcaa
     3061 tgcagtattg ttcttactct ttttttctgt tgcgacaggt tgtgacgtgg tgtccacggc
     3121 ctccctggtc tcttcaacca cggcggaggt tttttcttgt tccgtcatta tggcttcctg
     3181 ttatgagagt tattgtaatg cccgtaaaag cgcatcgttg tcagcgttat cggcgacctt
     3241 aatgtcttgc cagcccagtt cccgggcgag ttttgccaaa cgctcactga cgaccaatag
     3301 tcgacagtgt agtaaccagt gctcacgata ccattgtggg atcagcgacc agagttgctg
     3361 caacatttca ccgctggtaa caacgaccat cgtcacctcg cgggattgcc agcgcatcgc
     3421 ttcttctgca ccatcgtaat ggattgcgca tcgttgataa cattcacaaa aagtgacctc
     3481 agcaccgcgc accgtcaggg tatccccaat tagctcacga ccgccattgc cacgtaatat
     3541 cagcgcacgt ttgcccgcaa tattttgtaa ttcaggtaat tgtagcaaga cttcgctgat
     3601 ttcccgatcc tgtgggtaga gaatcttctg tccgcttacg gtatgtagcg ccagtgcggt
     3661 ggtgcgtcca atggcgaaat aagtaggtag ttggggccat ttacgatctt gctgatgcag
     3721 ctgtgattgg gcaaaagcaa ccgcgtgttg cgagagggca aacaacagat cgctctcccc
     3781 cagcgctgcc agttgatcag caagttgcgg taattgtcga cccggagaaa actcaatcag
     3841 cggaaaatgc caggccacct gccccagtgt gcgcagacgg ctcactaact cttctccagc
     3901 gggagacggg cgggtgacca ggatactcat gccggggcct ctccgttata gacttcagcg
     3961 aggatctcgc gcgcgccgtt attcagtagc tcttctgcca gcgaaatccc catttgttcg
     4021 gcatcttgcg gcgcaccgcg gcgttcaccg cgaataatct gcgaaccgtc cggcgcgccg
     4081 accagcgcac atagccagat ttcgccatca ataagctcgg cgtagctacc aattggcacc
     4141 tgacatccgc cttcgagacg ggtattcatg gcgcgttctg cggtaacgcg cagtgcagtt
     4201 tcgtggtgat tcagcgcggc aagcagctcg cgagtgcgtg tatcatcaag gcggcattca
     4261 ataccgactg caccttgtcc taccgccgga agagaaatct cgggtggtaa cgcggcgcga
     4321 atgcgggact ccagacctaa acgttttagt ccggctacgg caagaatgat ggcatcgtat
     4381 tcgccgttat ctagtttgct caggcgagtg ccgacgttgc cgcgcaggga gcggataatc
     4441 agatccgggc ggcgttcagc cagttggcac tggcgacgta aactggacgt cccgacgata
     4501 ctgcctgctg gtaacgcatc cagactgtca tagttattgg acacaaaggc atcgcgagga
     4561 tcttcacgct cgcaaatagt gaccagcccc agaccttgcg ggaattcaac cggcacatct
     4621 ttcattgaat gtacggcgat atcagcgcga ttctccagga gcgcgacttc cagctctttt
     4681 acaaataagc cttttccgcc tactttcgcc agcggcgtat caagaatcac atcgccgcgc
     4741 gtcaccatcg gtaccagttc aacgaccagg cccggatggc tcgccatcaa cttgtctttg
     4801 acatagtgtg cctgccagag tgcaagtggg ctttggcgtg tggcaattct taaaacattg
     4861 tctaacatgc ttgttaccgt cattatcatc cgtggtccat cctaacatcc ttgccagagt
     4921 gatgtcagtg ttgtggtgaa acgtagacgc ctgcgcaaac cgtaaaatga ggtctggcag
     4981 tggatcctga caggcgtttc acgccgtcgt aataaggaat ttacagagaa taaacggtgc
     5041 tacacttgta tgtagcgcat ctttctttac ggtcaatcag caaggtgtta aattgatcac
     5101 gttttagacc attttttcgt cgtgaaacta aaaaaaccag gcgcgaaaag tggtaacggt
     5161 tacctttgac atacgaaata tcccgaatgc cgcgtgttac cgttgatgtt ggcggaatca
     5221 cagtcatgac gggtagcaaa tcaggcgata cgtcttgtac ctctatattg agactctgaa
     5281 acagagactg gatgccataa atcaattgcg tgtggatcgc gcgcttgctg ctatggggcc
     5341 tgcattccaa caggtctaca gtctactgcc gacattgttg cactatcacc atccgcgaat
     5401 gccgggttac cttgatggta acgttcccaa aggcatttgc ctttacacgc ctgatgaaac
     5461 tcaacgccac tacctgaacg agcttgaact gtatcgtgga atgtcagtac aggatccacc
     5521 gaaaggtgag cttccaatta ctggtgtata caccatgggc agcacctcgt ccgtagggca
     5581 aagttgttcc tctgacctgg atatctgggt ctgtcatcaa tcctggctcg atagcgaaga
     5641 gcgccagtta ctacaacgta aatgcagcct gctggaaagc tgggccgcct cgctgggtgt
     5701 ggaagtcagc ttcttcctga ttgatgaaaa ccgcttccgt cataatgaaa gcggcagcct
     5761 ggggggcgaa gattgtggct ccacccagca tatactgctg cttgacgaat tttatcgtac
     5821 tgccgtgcgt ctcgccggta agcgtattct gtggaatatg gtgccgtgcg acgaagaaga
     5881 gcattacgac gactatgtga tgacgcttta cgcgcagggc gtgctgacgc caaatgaatg
     5941 gctggatctc ggtggcttaa gctcgctttc tgctgaagag tactttggtg ccagcctttg
     6001 gcagctctac aagagtatcg attccccata caaagcggtg ctgaaaacac tgctgctgga
     6061 agcctattcc tgggaatacc cgaacccacg tctgctggcg aaagatatca aacagcgttt
     6121 gcacgacggc gagattgtat cgtttggcct cgatccatac tgcatgatgc tggagcgtgt
     6181 tactgaatac ctgacggcga ttgaagattt cacccgtctg gatttagtac gtcgctgctt
     6241 ctatttaaaa gtgtgcgaaa aactcagccg tgaacgcgcc tgtgtaggct ggcgtcgcgc
     6301 agtgttgagc cagttagtga gcgagtgggg ttgggacgaa gctcgtctgg caatgctcga
     6361 taaccgtgct aactggaaga ttgatcaggt gcgtgaggcg cacaacgagc tgctcgacgc
     6421 gatgatgcag agctaccgta atctgatccg ctttgcgcgt cgcaataacc ttagcgtctc
     6481 cgccagtccg caggatatcg gcgtactgac gcgtaaactg tatgccgcgt ttgaagcatt
     6541 gccaggtaaa gtgacgctgg taaacccgca gatttcaccc gatctctcgg aaccgaatct
     6601 gacctttatt tatgtgccgc cgggccgggc taaccgttca ggttggtatc tgtataaccg
     6661 cgcgccaaat attgagtcga tcatcagcca tcagccgctg gaatataacc gttacctgaa
     6721 taaactggtg gcgtgggcat ggtttaacgg cctgctgacc tcgcgcaccc gtttgtatat
     6781 taaaggtaac ggcattgtcg atttgcctaa gttgcaggag atggtcgccg acgtgtcgca
     6841 ccatttcccg ctgcgcttac ctgcaccgac accgaaggcg ctctacagcc cgtgtgagat
     6901 tcgccatctg gcgattatcg ttaacctgga atatgacccg acagctgcgt tccgcaatca
     6961 ggtggtgcat tttgatttcc gtaagctgga tgtcttcagc tttggcgaga atcaaaattg
     7021 cctggtaggt agcgttgacc tgctgtaccg caactcgtgg aacgaagtgc gtacgctgca
     7081 cttcaacggc gagcaatcga tgatcgaagc cctgaaaact attctcggca aaatgcatca
     7141 ggacgccgca ccgccagata gcgtggaagt cttctgttat agccagcatc tgcgcggctt
     7201 aatccgtact cgcgtgcagc aactggtttc tgagtgtatt gaactgcgcc tttccagcac
     7261 ccgccaggaa accgggcgtt tcaaggcgct gcgcgtttct ggtcaaacct ggggcttgtt
     7321 cttcgaacgc ctgaatgtat cggtacagaa gctggagaac gccatcgagt tttatggcgc
     7381 tatttcgcat aacaaactgc acggcttgtc agtgcaggtt gaaaccaatc acgtcaaatt
     7441 accggcggtg gtggacggct ttgccagcga agggatcatc cagttctttt tcgaagaaac
     7501 gcaagacgag aatggcttta atatctacat tctcgacgaa agcaaccggg ttgaggtgta
     7561 tcaccactgc gaaggcagca aagaggagct ggtacgtgac gtcagtcgct tctactcgtc
     7621 atcgcacgac cgcttcacct acggctcaag cttcatcaac ttcaacctgc cgcagttcta
     7681 tcagattgtg aaggttgatg gtcgcgaaca ggtgattccg ttccgaacaa aatcgattgg
     7741 caacctgccg cccgccaatc aggatcacga tacgccgcta ttacagcagt atttttcgtg
     7801 atgagcgtgc cggaaagcga ggcttatccg gcatgcaatc ttagcggaaa ctgactgttt
     7861 cacccgcctg ttgcgtcgcc gcctgttcca gcaaatccca gaaggtttcg ccgctgcgat
     7921 cgcaaatcca ttcatcgcct ttcaggtcaa aatggtagcc gccctgtttg gttgccagcc
     7981 acacctggtg cagcggctcc tggcggttaa taatgatttt gctgccgttt tcaaaggtaa
     8041 tggtcagtac gccgccgttg atttcgcagt cgatatcgct gtcgccatcc ctgtcgtcca
     8101 gacgctcttc aatggtcagc cacagttgat cagccaggcg atgaaattca ctgtcgttca
     8161 ttgttgtatc ctgtttttaa gtgatggcgg cagtatagcg gcatggggtc agggcttcaa
     8221 agtttgcacc tctgctgctg cgttccggca tgattcatcc gtcaccggaa taatgatgtc
     8281 tctgttttga acttcacgta cgttctcctc tctgacacga gttttcgccg ggcaggggga
     8341 gggcgtaatc cttacctctg tatcggtcca tgtataaaca ttgcgatcgt cataagcgaa
     8401 gcgatcgttt ttagttagcg aaccgttaac tggaataatt tgcaagctct ttgaccgtaa
     8461 ttggcggata aagtaagcat gctgtttgcc gctattaaat gagcgatcaa tgaccaggtc
     8521 tggcgtatcc agcgggatta tctcaagctg gtcttcacca aatgtcgata attttgttac
     8581 atacagccga tctttatatt gcgcaatatt gccagtaatc agatggaatt
//
LOCUS       DADLTY010000174         8532 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.175, whole genome shotgun sequence.
ACCESSION   DADLTY010000174 DADLTY010000000
VERSION     DADLTY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.175"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..740
                     /gene="yihT"
                     /locus_tag="JN334_003464"
     CDS             <1..740
                     /gene="yihT"
                     /locus_tag="JN334_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HAY7439491.1"
                     /translation="FKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAAD
                     EFIPGNGIPVDSVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELC
                     HSHGLVSIIEPVVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQEL
                     LCASQRLNDHINMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDN
                     ELMLRDVCAPKLQQLGDIVDEMMAKRR"
     gene            757..1998
                     /gene="yihS"
                     /locus_tag="JN334_003465"
     CDS             757..1998
                     /gene="yihS"
                     /locus_tag="JN334_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HAY7439492.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            2112..3038
                     /locus_tag="JN334_003466"
     CDS             2112..3038
                     /locus_tag="JN334_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HAY7439493.1"
                     /translation="MSLIKVPCMQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTF
                     QGCHLVIPHKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGL
                     LAWRDWQISELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTV
                     AAPYGVGIHPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKK
                     IAATKIDHTFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMS
                     CPPNAFNSGIDLLLLEPGKPHRLFFNIYGQRK"
     gene            3237..5273
                     /gene="yihQ"
                     /locus_tag="JN334_003467"
     CDS             3237..5273
                     /gene="yihQ"
                     /locus_tag="JN334_003467"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HAY7439494.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            5319..6693
                     /locus_tag="JN334_003468"
                     /pseudo
     CDS             5319..6693
                     /locus_tag="JN334_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            6736..8139
                     /locus_tag="JN334_003469"
     CDS             6736..8139
                     /locus_tag="JN334_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7439495.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVEFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            8207..>8532
                     /locus_tag="JN334_003470"
     CDS             8207..>8532
                     /locus_tag="JN334_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAY7439496.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYL"
BASE COUNT         2069 a         2107 c         2167 g         2189 t
ORIGIN      
        1 atttcaaagt taacgctgca aaggccctct cgccttatgc ctcggcgatt ctggtagatc
       61 aacaattctg ctaccgccag gtggttgagc aaaacgcgat tgccaaaagt tgcgccatga
      121 ttgtcgccgc cgatgagttc attcctggca acggtattcc ggtcgatagc gtggttattg
      181 accgcaaaat caatccgcta cagatcaaac aagacggtgg caaagcctta aaactgctgg
      241 tgctgtggcg tagtgatgaa gatgcgcagc aacgtctgga tatggtgaaa gagttcaacg
      301 aactgtgcca ctcacacggt ctggtaagca tcattgagcc agtcgtccgc ccaccgcgtc
      361 gtggcgataa attcgatcgc gaacaagcga tcatcgatgc cgccaaagag ctgggcgaca
      421 gtggcgctga cctctacaaa gttgaaatgc cgctttatgg caaaggtccg caacaagagc
      481 ttctctgtgc ttcacaacgt ctgaatgacc atatcaatat gccatgggtg atcctctctt
      541 ccagtgtcga cgaaaaactg ttcccgcgtg ccgtacgcgt ggcaatgacg gcgggcgcat
      601 cgggatttct ggcaggccgt gcagtctggg catcggtcgt cggtttacca gacaacgagc
      661 tgatgctccg tgacgtttgc gcaccgaaat tacaacaact tggcgatatc gtcgacgaaa
      721 tgatggctaa acgccgctaa gatgaggata aaaggaatga aatggtttaa caccctaagc
      781 cacaaccgtt ggctggaaca ggaaaccgac cgcatctttg attttggtaa aaattccgta
      841 gtgccgactg gttttggctg gttaggcaat aaagggcaaa tcaaagaaga gatgggcacc
      901 catctgtgga tcacagctcg tatgttgcac gtttattccg ttgctgcggc gatgggtcga
      961 cctggcgctt acgcgttggt tgatcacggt attaaagcca tgaacggcgc actgcgtgat
     1021 aaaaaatatg gcggctggta tgcctgcgtg aatgacgagg gcgtggtgga tgcctccaaa
     1081 cagggctatc aacacttctt tgctctgctg ggtgccgcca gcgccgtcac aacgggtcac
     1141 ccggaagcgc gcaagctgct cgattacacc attgagatta tcgagaaata tttctggagc
     1201 gaagaagagc agatgtgcct ggagtcctgg gacgaagcct tcagcaaaac cgaagagtac
     1261 cgcggcggca atgccaatat gcacgcggtg gaagctttct tgattgttta tgacgtcact
     1321 cacgacaaaa aatggctgga tcgcgcgatt cgcgtggctt ccgtgattat ccacgacgtc
     1381 gccagaaata atcattatcg cgttaacgaa cacttcgata cccagtggaa tccgctgccg
     1441 gattacaaca aagataaccc ggcgcatcgc ttccgcgcgt tcggaggtac accaggccac
     1501 tggatcgaat ggggccgttt aatgctgcac atccatgctg ccctggaagc ccgttgcgaa
     1561 caaccaccag catggctgct agaagatgcc aaaggtctgt ttaacgccac cgtgcgcgat
     1621 gcctgggcac ccgatggagc ggacgggatt gtttataccg ttgactggga aggaaaaccg
     1681 gtggtccgcg aacgtgtacg ttggcctatc gtcgaagcaa tgggtacggc ctacgcgctc
     1741 tacaccgtca ctggcgatcg ccagtatgaa acctggtatc aaacatggtg ggagtactgc
     1801 attaagtacc tgatggacta tgaaaatggt tcctggtggc aggagctgga tgcggacaat
     1861 aaggtcatca ccaaagtctg ggacggcaaa caggatattt atcacctgct gcattgcctg
     1921 gtgatcccgc gtatcccgtt agcgcccggc atggctccag cggttgcagc gggtctgctg
     1981 gatattaatg cgaaataact gatctgaaaa tagggtgcgg gttttcccct caccctaacc
     2041 ctctccccaa aggggcgagg ggaccgtccg gtgcagtttt tgggtttgac atcagcctca
     2101 cgggaggctg tatgtcgtta attaaggtgc cttgtatgca aatcaccaat atgcattgca
     2161 gtggacagac cgtttcactt gccgcggggg attatcacgc gaccattgtg accgtcggtg
     2221 ctggtctggc tgaattaacc tttcagggtt gtcatttagt catcccgcac aaacccgaag
     2281 agatgccgct ggcgcatctg ggtaaagtgc ttattccgtg gccgaaccgg atcgccaacg
     2341 gctgttaccg ttatcagggg caggaatatc agttacccat taatgaacat agttcaaaag
     2401 ccgctattca cgggctgctt gcctggcgag actggcaaat tagtgaatta accaccacca
     2461 gcgttaccct gacagctttt ctgccgccca gttacggcta tccgtttatg ctggcttcac
     2521 aagttgttta ttcgctcaac gcacacacgg gtctttccgt tgagatcgca agccagaata
     2581 tcggcactgt cgcagcgcct tatggcgtgg gtattcatcc ttatttaacc tgcaacttaa
     2641 catctgttga tgaatatctg ttccagcttc cggcaaacca ggtttatgcc gtcgatgaac
     2701 acgtaaatcc gacaacgctt caccacgttg atgagctgga tttaaatttc acgcaggcga
     2761 aaaaaatagc cgcgacaaaa atcgaccaca cctttaaaac cgctaatgat ttgtgggaaa
     2821 tgaccattac ccatccacag caggcgcttt cggtaagcct ttgttccgat caaccctggg
     2881 tgcaggttta tagcggtgaa aaattacagc gtcagggact ggcagttgaa cctatgagtt
     2941 gcccgcctaa cgcctttaat tcaggtatcg acttattatt actggaacca ggaaaaccgc
     3001 accgcctgtt ttttaatatt tacggacaga gaaaataact tgtattgatt agcagtcaaa
     3061 atcattgaag aacatttcac tcgctaaagg caacattatg tatcttcaat ttattaagtt
     3121 aaaaacagct cagcttctgt aaagttttta tatatcagtt ttatgagaat cattttacat
     3181 aagcctgtaa taacaggaag gcaacaatct cgttctcaac tctatggaga ttagttatgg
     3241 atacgccacg tccacagtta ttagattttc aatttcatca gaataacgac agttttaccc
     3301 tacattttca acaacgtctt attttaaccc ataacaaaga taatccttgt ttatggattg
     3361 gctcaggtat agcggatatc gatatgttcc gcggtaattt cagcattaaa gataaactac
     3421 aggagaaaat tgcgcttacc gacgccatcg tcagccagtc accggatggt tggttaattc
     3481 atttcagccg tggttctgac attagcgcca cgctgaatat ctctgccgac gatcagggcc
     3541 gtttattgct ggaactacaa aacgacaacc ttaaccacaa ccgtatctgg ctgcgccttg
     3601 ccgctcaacc agaggaccat atctacggct gcggcgaaca gttttcctac ttcgatctgc
     3661 gcggcaaacc gttcccgcta tggaccagtg aacaaggcgt tggtcgcaac aaacaaacct
     3721 atgtcacctg gcaggccgac tgcaaagaaa atgcgggcgg cgactattac tggactttct
     3781 tcccacagcc tacgtttgtc agcacgcaga agtattactg ccatgttgat aacagttgct
     3841 acatgaactt cgactttagc gccccggaat accatgaact ggcgctgtgg gaagacaaag
     3901 ctacgctacg ttttgaatgt gctgacacat acatctccct gctggaaaaa ttaactgccc
     3961 tgctgggacg ccagccagaa ctgcccgact ggatttatga cggggtaacg ctcggcattc
     4021 agggcgggac ggaagtgtgc cagaagaaac tggacaccat gcgtaacgcg ggcgtgaagg
     4081 ttaacggcat ctgggcgcag gactggtctg ggatccgtat aacctctttt ggcaagcgcg
     4141 tgatgtggaa ctggaagtgg aacagcgaaa actacccgca actggattca cgcattaagc
     4201 agtggtatca ggagggcgtg cagttcctgg cctatatcaa cccgtatgtt gccagcgata
     4261 aagatctctg cgaagaagcg gcacaacacg gctatctagc aaaagatgcc tctggcggtg
     4321 actatctggt ggagtttggc gagttttacg gcggcgttgt cgatctcact aatccagaag
     4381 cctacgcctg gttcaaggaa gtgatcaaaa agaacatgat tgaactcggc tgcggcggct
     4441 ggatggctga cttcggcgag tatctgccca ccgacacgta cctgcataac ggcgtcagtg
     4501 ccgaaattat gcataacgcc tggcctgcgc tgtgggcgaa gtgtaactac gaagcccttg
     4561 aagaaacggg caagctcggc gagatcctct tctttatgcg cgccggttct accggtagcc
     4621 agaaatactc caccatgatg tgggcgggcg accagaacgt ggactggagt ctcgacgatg
     4681 gcctggcgtc cgtagtcccg gcggcgctgt cgctggcaat gaccggacat ggtctgcatc
     4741 acagcgacat tggcggttac accaccctgt ttgagatgaa gcgcagcaaa gagctgctgc
     4801 tgcgctggtg cgatttcagc gccttcacgc cgatgatgcg cacccacgaa ggtaaccgtc
     4861 ctggcgacaa ctggcagttt gacggcgatg cggaaactat cgcccatttt gcccgtatga
     4921 ctaccgtttt caccaccctg aaaccttatc tgaaagaggc cgtcgcgctg aatgcgaagt
     4981 ccggtctgcc ggtcatgcgc ccgctgttcc tgcattacga agacgatgca catacctatt
     5041 ccctgaaata tcagtacctg ttaggccgcg acattctggt cgctccggta cacgaagaag
     5101 gtcgcagcga ctggacgctc tatctgccgg aggataactg ggtacatgcc tggacgggtg
     5161 aagcgttccg gggcggggaa gttaccgtta atgcgcccat cggcaagccg ccggtctttt
     5221 atcgcgccga tagcgaatgg gcggcactgt tcgcgtcgtt aaacagcatc taatcacatc
     5281 cgcccgtggg gaaactcatg ggcaaaggga gaacatcgat gagtcacatc acaacggaag
     5341 atccagcaac tttacgcctg ccctttaaag agaaactctc ttacggtatt ggcgacctgg
     5401 cctctaacat cctgctggat atcggtacgc tttatctttt gaagttttat accgacgttc
     5461 tggggctgcc aggcacctat ggcggcatta tctttttgat ttcaaaattc tttactgcgt
     5521 ttaccgatat gggtaccggc attatgttgg attcccgacg caagatcggt ccaaaaggta
     5581 agttccgtcc ttttattctg tatgcgtcat tcccggtcac cttactggcg atcgccaact
     5641 ttgtcggcac accgtttgat gtcaccggta aaacggtgat ggccactatt ctgtttatgc
     5701 tttacggact gtttttcagc atgatgaact gctcctacgg cgcaatggtc ccggcaatca
     5761 ccaaaaaccc caacgaacgc gcctcactgg cggcatggcg tcagggcggc gctacgctgg
     5821 gcctgctgct gtgcacggtg ggattcgtgc cagttatgaa tcttatcgaa ggtaatcaga
     5881 aacttggcta tatcttcgcc gccacgctgt tttcactgtt cggcctgctg tttatgtgga
     5941 tctgctactc aggcgtgaaa gagcgttatg tcgaaaccca accaaccaat ccggcgcaaa
     6001 agcctggcct gttgcaatct ttccgcgcaa ttgccggtaa ccgcccactg ttcattctgt
     6061 gtattgccaa cctctgcacc ttaggggcgt ttaacgtcaa gctcgccatt caggtctatt
     6121 acacccagta cgtacttaac gatcccatcc tgttgtcgta tatgggattt ttcagcatgg
     6181 gctgtatttt catcggcgtg ttcctgatgc ctggcgcagt cagacgtttt ggtaagaaga
     6241 aggtctatat cggcggcctg ctgatttggg tgctgggcga tctgctcaac tatttcttcg
     6301 gcggcggctc ggtcagcttt gtggcgttct cctgcctggc gttcttcggc tcagcgcctg
     6361 aactgggcgc tggtttccga caccgtcgag tacggcgagt ggcgcaccgg cgtgcgttcg
     6421 gaaggcacgg tctacaccgg cttcaccttc tttcgcaaag tgtctcaggc gctggctggt
     6481 ttcttccccg gctggatgct gacgcaaatt ggctatgtgc cgaacgtcgc acaggctgac
     6541 cacaccattg aagggttacg ccagttgatc ttcatctacc caagcgcact ggcggtagtc
     6601 accattgtgg cgatgggttg cttctacagc ctgaacgaga agatgtatgt ccgcattgtg
     6661 gaagagatag aagcccgtaa acgcacggcg taataataat aaacgacgcc ctgcggggcg
     6721 ttataaggag tgattatgtc tgaccataat ccactgacat taaaactgaa tctgcgggaa
     6781 aaaatcgcct atggtatggg cgacgtcggt tcgaatttaa tgctctgcat cggtactctg
     6841 tatctcctca aactttatac cgatgaatta gggatgcctg cttactatgg cgggattatc
     6901 tttctggtcg cgaagttttt taccgcgttt accgatatgc tcaccggatt tttactcgac
     6961 tcgcggaaaa atattgggcc taaaggcaaa tttcgtccct ttattttata tgcagcagtt
     7021 ccggcggcgt taattgcaac gctccagttt atcgccacca ccttttgttt gccggttaaa
     7081 acgacgattg ccaccacgct gtttatgatg tttggacttt catacagtct gatgaactgc
     7141 tcgtatggtg cgatgatccc ggcaattacc aaaaacccga atgagcgcgc gcagctcgcg
     7201 gcttaccgcc agggtggtgc aaccataggg ctattgattt gtaccgtgga gtttattccg
     7261 ttgcagtcgc ttttttctga ctcaaccgtc ggttatgcct gtgcggcact tatgttctcc
     7321 attggcggct ttatttttat gatgctgtgc tacagaggcg tcaaagagca ttatgtggac
     7381 acagcgccaa ccggacataa agccagtatt ctcaaatctt tttgcgcgat atttcgtaat
     7441 ccaccattgc tggttttatg cattgctaac ctgtgtactc tggcggcatt taatatcaaa
     7501 ctggcgattc aggtctatta cacccagtat gtgctgaatg atattaattt attgtcgtgg
     7561 atgggattct tcagtatggg atgcatcctc gtcggcgtat tactggtgcc agtaactgta
     7621 aaatgttttg gtaaaaaaca ggtttattta gctggcatgg tgctatgggc ggtgggtgat
     7681 atactgaatt atttctgggg aagtaactct ttcactttcg tcatgttctc ttgtgtcgcc
     7741 ttttttggca cggcctttgt aaacagcctg aactgggcac tggtaccaga taccgtagat
     7801 tacggtgaat ggaaaaccgg tattcgtgcc gaaggttctg tttataccgg ttataccttc
     7861 tttcgtaaaa tttctgccgc acttgctggc ttcttgccag gcattatgct gacgcaaatt
     7921 ggttatgttc ccaacatcgc gcaaagcgat gcgacattac agggtttgcg tcagctcatt
     7981 tttatctggc cttgtgcact ggcaattatt gctgcattga cgatgggatt cttttacaca
     8041 ctcaatgaaa aacggtttgc attgattatt gaggaaatca accaacgcaa aaataaagag
     8101 atggcgacag aaaaaaaaac ggcttccgta acgttataaa tgtcaatagc cgctatttcc
     8161 atcctggtgg gtggcggcct ccctacgttt aaaaaatgga cttattatga aaaagattaa
     8221 tgcaataatt ttattatctt ctctgacctc tgcctcggta tttgctggcg catacgtcga
     8281 aaacagggag gcatataatc ttgcctccga tcagggcgag gttatgctac gtgtgggtta
     8341 taacttcgat atgggcgcgg gtattatgtt aaccaatacc tacacctttc agcgagaaga
     8401 tgaactaaaa catggctata atgaaattga aggttggtat cctttattca aaccaaccga
     8461 taaattaacc atccagcccg gtggcttaat taatgataag agtatcagtt cgggtggtgc
     8521 agtgtatctg ga
//
LOCUS       DADLTY010000175         8481 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.176, whole genome shotgun sequence.
ACCESSION   DADLTY010000175 DADLTY010000000
VERSION     DADLTY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.176"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            44..397
                     /locus_tag="JN334_003471"
     CDS             44..397
                     /locus_tag="JN334_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HAY7439497.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDYKRP
                     VPELPEKYRPKKPRE"
     gene            complement(434..2512)
                     /gene="flhA"
                     /locus_tag="JN334_003472"
     CDS             complement(434..2512)
                     /gene="flhA"
                     /locus_tag="JN334_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HAY7439498.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="JN334_003473"
     CDS             complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="JN334_003473"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HAY7439499.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="JN334_003474"
     CDS             complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="JN334_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAY7439500.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="JN334_003475"
     CDS             complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="JN334_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HAY7439501.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="JN334_003476"
     CDS             complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="JN334_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAY7439502.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="JN334_003477"
     CDS             complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="JN334_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY7439503.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6416..6877
                     /gene="hycA"
                     /locus_tag="JN334_003478"
     CDS             6416..6877
                     /gene="hycA"
                     /locus_tag="JN334_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HAY7439504.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7002..7613
                     /locus_tag="JN334_003479"
     CDS             7002..7613
                     /locus_tag="JN334_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7439505.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7650..>8481
                     /locus_tag="JN334_003480"
     CDS             7650..>8481
                     /locus_tag="JN334_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708524.2"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HAY7439506.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     T"
BASE COUNT         1899 a         2305 c         2282 g         1995 t
ORIGIN      
        1 attaagatgt aatataaatg catcttaatg aggtggaggc agcatgtccg gcaagcgtat
       61 ctctcgtgaa aaactgacga ttaaaaaaat gatcgatctt tatcaagcga aatgcccgca
      121 ggcgtcagcg gagccggagc attacgaggc gttgtttgtt tacgcgcaaa agcggctgga
      181 taaatgtgtg ttcggcgaag agaaaccagc ctgtaagcag tgtccggtgc actgttacca
      241 gcctgcaaag cgtgaagaga tgaaacagat tatgcgttgg gccgggccga gaatgctgtg
      301 gcgtcatccg atcttaaccg tgcgtcatct catcgattac aagcgtccgg tgccagaact
      361 gccggaaaaa tatcgtccga aaaaacctcg tgaatagtat gccggacagg cagatctgtc
      421 cggcaatttg cagttaaatc aatgccgatt tatcaattcc cagccgcttc atccgtgaca
      481 gcagggtcgt gcgtttcagc cccagacgtt gcgcagcgcc tttaggcccg gcaaccacgc
      541 tgttggtttc tttcagcacg cgcacaatca actgatattc atcttcgccc tcctgggcga
      601 caaccgttgc ggcaggcggc gtttcaggct ccggtaaagc aatatctggc aatgacagct
      661 gcagcacgtt gccgcgtgtt agcaatactg cgcgctcaat gacgttttcc agttcgcgta
      721 cgttacccgg ccactccatg ttgctcaagg tgcgcagcgt ctcggcagga atgctgtcga
      781 tattgcgccc cagacgacgg gcaattttga aggtaaaggc tttcgccagc agcggaatat
      841 cttccggacg ctcgcgtagt ggcggcaggt gaatcgggaa tacgttcagg cggtaataga
      901 gatcgctacg gaactctcgg tcggcgacca tttttttcag atcgcggtta gtcgcggcaa
      961 ttaaacgtac gtccgtctga atgattttgt tgctgccgag acgttcaaac tcctgttcct
     1021 gcaatacacg cagcaacttc ggctgtaact ccagtggcat atcgcccact tcgtcgagga
     1081 acagagagct tttatccgcc agttcaaaac gaccaatgcg ctgggcgttg gcaccggtaa
     1141 aagccccacg ctcatgacca aacagatcgc tttccagcaa tccggcaggc atcgccgcgc
     1201 agttcatttt gaccatgcgg cgattattac gcccactgag attatggatc gcacgggcaa
     1261 tcagctcttt acccgagcca gtttcaccga ggatcagcac ggtactgtca ctttgcgcca
     1321 ccatttcaac ttgtttaagc acgctgtaca tggcttcgct gcggccaata atctcgccaa
     1381 attcactatc aacattgttg agctgctcgg tcagggcgag gttttcatca accagccgtt
     1441 ctttcagacg atggatttcc tgataggcga gggcgttatc gacagcgatt gccacacgtt
     1501 cggcaatctg gcgcagcaaa ttcagattgg tagtggtaaa cactttctct tcgcattgcg
     1561 ccagtttcag cacgcccagc atggtgtcgc cagacatcag cggtaacagg cacaaggttt
     1621 gaatctggtt gccccaggtg tcgaacaaca tgcgttcata gggggctaaa tcgtcccgct
     1681 cgtagagatt gatcagcagc atctctttac ttttgaacac gcgttcggtg agggttccgg
     1741 cttcatcgac ttcgctctgt tcgtgggcgg gatgttgttt atcaagatag tgagtggagt
     1801 agatgttgag tttgttttta cggtggctgc gtaagacaat gctgatatcg tcaatgtcga
     1861 aatagtaatg gatttctttg gcgacttcgc tgaccagttc gtccatatcc aggcgggaaa
     1921 gcaccgcgtt ggtaatggca accaggatgc ggaagttatc gcgctcccgg cataacaact
     1981 catagtcgac attgttgacg acgcggctct ggatttgttc ggtgacgaca gaaacgatct
     2041 gcgtaaatgt ttgcagacga ttgaactctt tttcgctcca gggacgctcg tcataacgaa
     2101 taaattcaca gccaccaaaa atatgccctc ccgccgccag tggcatcagg caatagtgac
     2161 caaatttggg gtatagccca cctgcggcca gctgcggcca ggtttcacaa aattcttcgt
     2221 aactgcaatg cagcgtatca gggcgcgaca aaatgctgcg taccggaccg tgtgccagaa
     2281 cagtttcgtc ttcatattta atgggggtgt ctttttcacg cgacgcgtaa taagacgcac
     2341 gttgagtctg cgcttgccac aacacaatag ccgcgttgtc ggcgagcgca gaacgcttta
     2401 ccagttgcga aagagcctca cacagcgagg ccagatcggg ctgctgcaat agtgtccgag
     2461 tgatgtcgaa caacccttgc tgtccgagat cactcatcgg tgtatatgac attttgttct
     2521 tccaggaaag caccgcaaag cggtgcaaaa ggtcattgac taactatagg agatttagaa
     2581 ttttattagc atatacgcgg aagcggttcg gcgtgtggta aatcgagggt tcgtttcacg
     2641 ccatacagac cggcaagacg aacaccttta cgttccacca cttcaccaat cagcgccgcg
     2701 tctttcccca gtggatggga atgtaacgct gccagcactt gctctgccgc gttgcgttca
     2761 acggcgatca ccagtttgcc ttcgttggca aagttcaggg cgtccagtcc cagcaattcg
     2821 caaacgccgc gcacggcagg tttaaccggc agcgctgatt cagaaatttc aataccacaa
     2881 ccgcaggctg ccgcgaactc atgaaccacc gcgtttacac caccacgggt ggcatcacgc
     2941 agcgctttca cgccgggaat gtcacgcagc gtctgaataa gcggcgtcag caccgcgcag
     3001 tcgctgacca gttcgccatc cagccccagc tgctcacgca ggttaaggat agtcgccccg
     3061 tggtcgccga gtgtaccgct aaccagcaat acatcgcctg cggttagcgt ctgtgcgccc
     3121 cagtgaatat tcgccggaat tgcgcccatg ccagcggtgt tgataaacag tttatctgcc
     3181 gcgccgcgct gcaccacttt agtatcgcca gtaacgatgg caatgcctgc cgtgcgggcg
     3241 gtttctgcca tgctggtcac tacggctttc agtgtctcca tcggcaatcc ttcttcgagg
     3301 ataaagccac aggagagata gcgcggaata gcgccactgc ccgcaacgtc attcgcggtg
     3361 ccgcaaatcg ccagcttgcc gatattaccg ccaggaaaga acagcgggtc aatgacgtaa
     3421 ctgtcggtgg agaacgccag acggtcgcct tccgctacca gctgcgccag atcaagacgt
     3481 gcctgatctt cctgctctgc cagccacggg ttggcaaagg cttccataaa caggctgttg
     3541 attaattgct gcatcgcctg gccgccgctg ccgtgggcga gttggatatt attcacgctt
     3601 cactctcctg ctgacgatac tgataccacg cggcgcacgc tccttcggag gagaccatca
     3661 gtgcaccaaa tgcggtttga ggattacagg tgttaccaaa cagcgggcat tgatgcggct
     3721 tacatttgcc cgttaatacc tcaccacaac gcgcgcgcgg gtcatcgcag acctgctgcg
     3781 gtgccgggcg gaaatgtgct tcggcatcga atcgttgata atccggcgtc aggtgcacgc
     3841 cagaagattc aatcacgcct aagccgcgcc attcgctgtc gccgttgaca cagaacacat
     3901 cggcaatcgc ctgttgcgcc agtaggttac cggcatccgg taccacccga cgatactgat
     3961 tctctacctt gctgtgggcc gctattttct gctccaccag catgaccacg ccttgcagta
     4021 gatcaagggg ttcgaaacca gcgaccacca gcggacgatg aaaatcgctg gcgataaaat
     4081 tataggcatc agtgccgata accatactaa cgtggcccgg cgcgaggaac gcgtcgatac
     4141 cgttatccgg ctgttccagc aaactgcgca gcgtcgggat aagcgtaatg tgctggcaga
     4201 agaagtaaaa attctgcaca tcacgcgctt tcgcctgttg cagagtgatg gcggtggtcg
     4261 gcatggtggt ttcaaaacct aagccgaaga acaccacttt gcgggttgga ttctcctgcg
     4321 ccagtttcaa cgcatccatc ggcgagtaaa cgatgcgcac atcggcaccg cgtgcttttg
     4381 cctgcaacag cgatccctgt ttccctggta cgcgcatggc gtcgccaaag gtacagaaga
     4441 tgacttccgg atggctggca atctccacgc aggtgtcgat tctacccatc ggcagtacgc
     4501 acaccgggca acccggaccg tggataaact caacgttttc aggcagtaac tggtcgaggc
     4561 cgaatttaaa aatggcgtgg gtatgaccgc cacacacttc cataatccgc agagggcgtt
     4621 cggcggtgta agagagatgt gaagcacgtt cgcgcagatg ctcaattaac tgcatcacct
     4681 gttccggcgc gcgatattca tcaacaaaac gcattatttt tcctcgccat acaacagcgc
     4741 gccgacatcc ggctcaacgt caaacatgtt ttgtaaggcg tcgagagtgt cgcgtgcttc
     4801 ggcttcatta attacgctca tggcaaagcc aacgtgaacc agtacccact ggcccacgcg
     4861 cggctgaccg ttttcatcgc agctgccgac taacgttaaa tcgacatcgc gctgaatgcc
     4921 gcagacgtcg actttcgcct ggttgccgtc aatggtgcgg atctggccgg gaacgcctat
     4981 gcacatcgct gtgtctccag ccagttcagc cactggtcca tcccttcgcc gctggtggcg
     5041 gaaataagga tgatttcaat ttctggattg acttcgcggg cgcaggcgat gcacttctca
     5101 acgtcaaagt tgagatacgg caacaggtca actttgttga gcagcatcag cgaggcggcg
     5161 gcaaacatat gcggatattt cagcggtttg tcttcacctt cggtaacgga aagcaccgct
     5221 actttgtgtt tttcaccgag atcgaagctg gccgggcata cgaggttgcc aacgttttcg
     5281 ataaacagaa taccgttatc gtccagtggc agacgcggtg cggcgtcggc aatcatctgt
     5341 gcgtcaagat ggcagccttt accggtgttc acctgaatcg ctggtgtacc ggtagcgcga
     5401 atgcgtgcgg catcgttcac ggtttgctgg tcgccttcaa taaccgcgca cggaacgctg
     5461 tctttcaggc gcattaaggt ttccgtcagc agggtggttt taccggaacc agggctggaa
     5521 accaggttga gcaccagttg cttgcgggca gcaaagcgcg cgcggttgcg ttcagccaga
     5581 cggttgtttt tgtccagcac gtcaatttcg acttccagca tccgacgctg gctcatgccc
     5641 ggtgtgtgag tgcccgcttc gccatgaccg taatgcaggt cgccttcttc agtctggctg
     5701 ggggtaaatt caggcgcttt aatgccggtg attttcatct gcgggcgtgc cgccggggca
     5761 aatggcgcgc tacgaaacgc ggaatgaggg ttatgttcat caccctcgat atacaggttg
     5821 ccttcaccgc aaccgcatgt tgtacacatc gctcactcct ggtctatttc tatccgccga
     5881 atctgtaaac cgtcgtctgc cacaatctgt agcatgtcac catgacactg tggacagcgg
     5941 cggacgcgct gggtcagtag cgtcacatac tgttggcatg tttcacacca gcattcggcc
     6001 tcttgttctt cgaggtgcag tttacaacct tccgccacgc tgccgcggca aaccagatca
     6061 aaacaaaagg caagagagct ggtttcgaca caagaaaatg cgccaatttt gagccagacc
     6121 ccagttacgc gttttgcgcc gtgttttgcg gcctgctgtt cgatcaattc cagtgcccgt
     6181 tggcagaggg ttatttcgtg catatcgcct cccattaact attgccagct acaagcaata
     6241 attgcgccag tgttgattat ccctgcggtg aataatgtcg atgatgtcga aatgacacgt
     6301 cgacacggcg acgaaattca tctttagctt aaaaatctct ttaataacaa taaattaaaa
     6361 agttggcaca aaaaatgctt aaagctggca tctctgttaa acgagtaacc tgacaatgac
     6421 tatttgggaa ataagcgaga aagccgatta catcgcacag cggcatcgtc gcctacagga
     6481 ccagtggcac atctactgca attcgctggt tcaggggatc acgttatcga aagcgcgcct
     6541 gcatcacgcc atgagctgcg cgccggacaa agaactctgt ttcgtccttt ttgaacattt
     6601 tcgtatttac gtcaccctgg cggatggctt taacagccac accatcgagt attacgtcga
     6661 aacaaaagat ggcgaagaca aacagcggat tgcgcaggcg caactgagca ttgacggcat
     6721 gattgatggc aaggtcaaca tccgcgatcg cgaacaggtt ctggaacact atctcgaaaa
     6781 aatcgctggc gtttacgaca gcttatacac cgccattgaa aacaatgtgc cggtgaattt
     6841 aagccaactg gtaaagggac aaagcccggc agcatgagct gaggctttgc ccgttttgca
     6901 ggcgttacgc ctgtttgggg atgggcgtgt cgatgagtgt cgaaaatgac atttcatcgg
     6961 catgttttcg tcaaaaatga caatcacctg aggaatgcct ggtgaatcgt tttgtaattg
     7021 ccgactccac gctctgtatc ggctgccaca cctgtgaggc cgcctgttca gagacgcatc
     7081 gccagcacgg cctgcaatca atgccgcgcc tgagagtgat gctcaatgaa aaagaatctg
     7141 cgccgcagct ctgtcaccac tgtgaagatg caccctgcgc ggtggtctgc ccggttaacg
     7201 ccatcacccg cgttgatggg gccgtgcagt tgaatgaaag tctgtgcgta agctgcaaac
     7261 tgtgcggcat cgcctgcccg tttggcgcaa ttgaattttc cggcagccgt ccgctggata
     7321 ttccggcaaa cgccaatacc ccgaaagcgc caccggcacc gcctgctccg gcgcgtgtca
     7381 gcacattgct tgactgggtg ccaggtattc gcgcgatcgc cgtcaaatgt gacctttgta
     7441 gctttgatga acaaggtccg gcctgtgtgc ggatgtgccc gactaaagcc ctgcatctgg
     7501 tggataacac cgatatcgcc cgcgtcagca aacgtaagcg tgagctgacc tttaacacgg
     7561 actttggcga tctcaccttg tttcagcagg ctcaaagtgg agaggctaaa tgagcgcaat
     7621 ttccctgatc aatagcggcg tggcatggtt tgtcgccgcc gctgttctgg catttctctt
     7681 ttcaaaaagc gctaagtggc tggatagccg gaattggcgg cgcggttggt agtctgtata
     7741 cggcagccgc gggcttcact gtactgactg gcgcggttgg cgtgagcggt gcgctgtcgc
     7801 tggtaagcta cgatgtgcaa atctccccgc ttaacgcgat ttggctgatt accctcggcc
     7861 tttgcggtct gtttgtcagc ctctataaca ttgactggca tcgccacgcg caggtgaagt
     7921 gcaacggctt gcagatcaat atgttgatgg ctgccgccgt ctgcgccgtc atcgccagca
     7981 acctcggcat gttcgtggta atggccgaag tcatggcctt gtgtgcggtg ttcctcacca
     8041 gcaacagcaa agagggcaaa ctgtggtttg cgctgaggcg tcttggcact ctgctgctgg
     8101 tgattgcttg ctggctgctg tggcagcgtt atggcacgct ggatctgcgc ctgctggata
     8161 tgcgtatgca acagctgccg ctcggttccg atatctggct gctcggtgtg attggctttg
     8221 gcctgctggc tgggattatt ccgctgcacg gctgggtgcc gcaggcacat gctaacgcct
     8281 ctgcgccagc tgccgcgttg ttttctacgg tagtcatgaa aattggcctg ctgggcatgt
     8341 taaccctgtc actgctgggc ggtaatgcac cgctgtggtg ggggatcgcg ctgctggtgc
     8401 tcggcatgat caccgcgttc gtcggtggtc tgtatgcgtt gatggaacac aacatccagc
     8461 gcctgctggc ttaccacacc c
//
LOCUS       DADLTY010000176         8330 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.177, whole genome shotgun sequence.
ACCESSION   DADLTY010000176 DADLTY010000000
VERSION     DADLTY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.177"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..157
                     /locus_tag="JN334_003481"
     CDS             <1..157
                     /locus_tag="JN334_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="traD domain protein"
                     /protein_id="HAY7439507.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(166..564)
                     /gene="vapC"
                     /locus_tag="JN334_003482"
     CDS             complement(166..564)
                     /gene="vapC"
                     /locus_tag="JN334_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HAY7439508.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(564..791)
                     /gene="vapB"
                     /locus_tag="JN334_003483"
     CDS             complement(564..791)
                     /gene="vapB"
                     /locus_tag="JN334_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HAY7439509.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            873..1244
                     /locus_tag="JN334_003484"
     CDS             873..1244
                     /locus_tag="JN334_003484"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HAY7439510.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1265..6126
                     /gene="traI"
                     /locus_tag="JN334_003485"
                     /pseudo
     CDS             1265..6126
                     /gene="traI"
                     /locus_tag="JN334_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6146..6892
                     /gene="traX"
                     /locus_tag="JN334_003486"
     CDS             6146..6892
                     /gene="traX"
                     /locus_tag="JN334_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAY7439511.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6947..7507
                     /locus_tag="JN334_003487"
     CDS             6947..7507
                     /locus_tag="JN334_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAY7439512.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7638..7850
                     /locus_tag="JN334_003488"
     CDS             7638..7850
                     /locus_tag="JN334_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HAY7439513.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8034..>8330
                     /locus_tag="JN334_003489"
     CDS             8034..>8330
                     /locus_tag="JN334_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAY7439514.1"
                     /translation="MLHYSGGLKYRWHLSDMENNMRKYIPLALFIFSWPVLSADIHGR
                     VVRVLDGDTIEVMDSLKAVRIRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1912 a         2086 c         2671 g         1661 t
ORIGIN      
        1 cagtgaatcc ggtgaagtgg tggatatggc cgcttatgag gcatggcagc aggaaaatca
       61 tccggacacc tggcagcaga tgcagcgtcg tgaagaggtg aacatcaatg tgcaccggga
      121 acgcggggag gatgttgagc cgggagatga tttctgatga acaggtcagc tccagtcttc
      181 agttctcagg ccgcccacac gttcaaattc ccgggtgtta ttagtcacaa taatcagccc
      241 ccgactgcgg gcatgacctg cgatcatttg atcaaatggc ccgacagggc gtccctgacg
      301 ggcaagttct gctcttatct ggccggtgtg tgtggctgca gcagcgtcgt aatccagaac
      361 atcaatgcgg gaaacaaatc cctcgatcac agcgagatta cgttcaggca tctggctttt
      421 ttctgcacca tatatcagct ccatcagggt gaccgaactg atacacattt tcccctggtt
      481 caggttaaaa cgctccctga cgctggcggg tttgttcttt atcgtaaaaa tgcagatgtt
      541 ggtatcgagc ataaacttca gcatcagaat gactcccttt cttgcatgcc gggctgttcc
      601 ctgttatcca taaaatcggt gctgacgctg tgtccgtcga accattcgtc ccatgtctct
      661 ccggctggcg taatgattct ggttcgtccg acagcaatca cttcaacgcg ttttacgttt
      721 tccggcaatg caaccgcttt tggcagtctg accgcctggc tgcggttgct gagaaatacg
      781 gtggtttcca ttatttgcct ccttatgtgg atatgtcttg tgtatatcta ttgtgggatt
      841 gataccggga tatgtcaaag ggatatacgt ttatgatgag tatcgcgcag gtcagatcgg
      901 ccggaagtgc cggtaattat tataccgaca aggataatta ctatgtgctg ggcagcatgg
      961 gagaacgctg ggccggccag ggggctgaac aactggggct gcagggcagt gtcgataagg
     1021 atgtctttac ccgccttctg gagggcaggc tgccggacgg ggcggattta agccgcatgc
     1081 aggatggcag taacaggcat cgtcccggct acgacctgac cttctccgct cccaaaagta
     1141 tctccatgat ggccatgctc gggggggaca aacgtcttat tgaagcacat aaccaggccg
     1201 tggattttgc tgtccgtcag gtggaggcca gtgcctccac ctgacggacg gacagtcaga
     1261 aacggtgctg accggtaatc tggtgatggc actgtttaac cacgacacca gtcgcgatca
     1321 ggaaccacag ttacacacgc atgcggtggt gactaatgtt acgcagtata acggcgaatg
     1381 gaagacactg agcagtgaca aagtggggaa aacggggttc attgagaatg tgtacgctaa
     1441 tcagattgcc tttggcaggc tctaccggga aaaactgaaa gagcaggttg aggcgctggg
     1501 ctatgaaact gaagtggtcg gtaagcacgg tatgtgggaa atgccgggtg taccggtgga
     1561 ggccttttcc ggacgctcac agactatccg ggaggccgtc ggggaagacg cctcgctgaa
     1621 atcccgggat gtggcggcgc tggatacgcg taaatccaaa cagcacgtcg atcctgaagt
     1681 cagaatggcc gagtggatgc agacgctgaa ggaaaccggg ttcgacatcc gggcatatcg
     1741 tgacgcagcg gaacaacggg catatacccg cacgcagaca cccggacccg cttcacagga
     1801 cgggccggat gtgcagcagg cggtgacaca ggccattgcc ggattaagtg aacgcaaagt
     1861 gcagtttatg tatacggacc tgctggccag gacggtcggc atactgccgc cggaaaatgg
     1921 tgtgattgaa cgggcacgcg ccggtatcga tgaggccatc agccgtgagc agcttatccc
     1981 cctcgatcgt gagaaggggc tgttcacgtt cgggattcat atgctcgatg agctgtcagt
     2041 ccgggcactc agtcgtgaca tcatgaaaca gaaccgggtg accgtacatc cggagaaaag
     2101 tgtcccccgg acggccggtt acagcgatgc cgtcagtgtt ctggcacagg accgtccgtc
     2161 gctggccatt gtgtccgggc agggcggtgc agccgggcag cgtgagcggg tggctgaact
     2221 ggtcatgatg gcccgggagc aggggcggga ggtgcagatt atcgctgctg accgtcgctc
     2281 gcagatgaac ctgaagcagg atgaacggct gtccggtgaa ctgataaccg gacgtcgtca
     2341 gttgctggaa ggaatggcct tcacgccggg cagtaccgtt atcgtcgacc agggcgaaaa
     2401 actctccctg aaagagacgt taaccctgct ggatggtgcc gcacgtcata acgtacaggt
     2461 cctgataacc gacagcgggc agcgaaccgg tacaggcagt gcactgatgg ccatgaagga
     2521 tgccggggtg aacacatatc gctggcaggg gggagaacag cgaccggcca ccatcatcag
     2581 tgaaccggac cgtaatgtcg gctggccgga gattttgcgg ccagcgtgaa agccggagaa
     2641 gagagcgtgg cacaggtcag cggagtacgg gaacaggcca tactgacaca ggccattcgc
     2701 agtgagctga aaacacaggg cgtgctcgga cacccggagg taaccatgac cgccctgtca
     2761 ccggtctggc tggacagccg gagccgttat ctgcgggata tgtaccgccc gggaatggtg
     2821 atggagcagt ggaacccgga gacacgcagt catgaccgct atgtgacaga acgtgtgacg
     2881 gcgcagagtc acagcctgac cctgcggaat gcgcagggcg aaacgcaggt ggtgcgtatt
     2941 tcttccctgg acagcagctg gtcgctgttc cggccggaaa aaatgccggt ggcagacggt
     3001 gagcgtctga gggtgacagg gaaaattccc ggactccgcg tctccggcgg tgaccgcctg
     3061 caggtggcat ccgtcagtga agatgcgatg acggttgttg tgccgggacg ggctgaaccg
     3121 gccaccctgc ctgtgagcga ttcaccgttc actgcactga agctggagaa cggctgggtg
     3181 gaaacgcccg ggcattccgt cagcgacagt gcgacggttt ttgcctccgt cacacagatg
     3241 gcaatggaca atgccaccct gaatggtctg gcccgcagtg gtcgtgatgt ccggctgtat
     3301 tcctcactgg atgaaacccg tactgcggaa aaacttgccc gccatccctc ctttacggtg
     3361 gtttctgagc agataaaggc gcgtgccggt gagacattgc tggaaaccgc tatcagtctg
     3421 cagaaagccg ggcttcacac gccggcacag caggccattc atctggccct tcctgtgctg
     3481 gaaagtaaaa acctggcctt cagcatggtg gacctgctga cagaggcgaa gtcgtttgct
     3541 gcagaaggaa ccggttttgc tgacctggga ggggaaatca atgcgcagat aaaacggggt
     3601 gatttactgt atgtggatgt ggcaaaaggc tatggcacag gcctgctggt ttcccgtgcg
     3661 tcgtatgagg cagaaaagag cattcttcgc catattctcg aaggtaagga ggcggtcacg
     3721 ccgctgatgg agagagtacc tggcgaactc atggagaaac tgacatcagg acagcgcgcc
     3781 gccacccgta tgatactgga aacgtctgac cgtttcacgg tggtgcaggg ctatgccggt
     3841 gtgggtaaga ccacacagtt ccgggcggtg atgtcagccg tgaacatgct gccggagagt
     3901 gaacgtcccc gtgtcgtggg gctggggccc acacaccgtg cggtcgggga gatgcgcagc
     3961 gccggcgtgg atgcacagac actggcgtcc tttctgcatg acacgcagct gcagcagcgc
     4021 agcggagaaa ctccggattt cagcaacacg ctgttccttc tcgatgagag ctctatggtg
     4081 ggcaataccg acatggcacg ggcatacgcc ctgattgcgg ccggtggcgg tcgggctgtg
     4141 gccagcggtg acacggacca gctgcaggcc atcgcgcccg gtcagccttt ccgtctccag
     4201 cagacgcgca gtgctgccga tgtggtcatc atgaaggaga ttgtgcgtca gacgccggaa
     4261 ctgcgggagg cggtatacag cctgattaac cgggatgtgg aaagggcgct gtccgggctt
     4321 gagcgtgtga aaccgtctca ggtgccacgt ctggagggcg catgggcacc ggagcactcc
     4381 gtgacggagt tcagtcacag ccaggaagcg aaactggcag aagcgcagca gaaggcgatg
     4441 ctgaaaggcg aggcttttcc ggatgtcccc atgacactgt atgaagccat tgtccgcgac
     4501 tataccggca ggacaccgga agcacgggag cagacgctga ttgtcacgca cctgaatgag
     4561 gaccggcgcg tactgaacag catgattcat gatgcacggg aaaaggccgg agaactgggg
     4621 aaggtgcagg tcatggtgcc tgtcctgaac acagcgaata tacgcgacgg ggagctgcgt
     4681 cgtctctcca cctgggagaa taacccggat gcccttgccc tggtggataa tgtgtatcac
     4741 cggattgccg gtatcagtaa ggatgacggg ctgataaccc tgcaggatgc ggaaggtaac
     4801 acgcggctga tttcgccccg ggaggcggtg gctgaaggcg tcacgctgta caccccggac
     4861 accatccggg tggggaccgg tgacaggata cgcttcacga agagtgaccg ggagcgcggt
     4921 tatgtggcca acagcgtctg gacggtgaca gcggtttccg gtgacagtgt cacactttct
     4981 gacggtcagc agacccgtgt gattcgcccc ggtcaggagc gggcagagca acatattgac
     5041 ctggcctatg ccatcaccgc ccacggtgca cagggggcga gtgaaacctt tgccatcgcg
     5101 cttgaaggca cggaaggtaa ccggaaactg atggccggct ttgagtcagc ctacgtggcc
     5161 ctgtcgcgta tgaagcagca tgtgcaggtg tacaccgata accgtcaggg ctggacggat
     5221 gccatcaaca atgccgtaca gaaaggaact gcccacgatg tgtttgagcc gaaaccggac
     5281 cgggaggtca tgaatgcaga gcggctgttc agtacggcgc gggagctgcg ggacgtggcg
     5341 gcaggccgtg ccgttctccg tcaggcgggg ctggccgggg gagacagtcc tgcacggttt
     5401 attgctccgg gacgtaaata tccgcaaccg tatgtggcac tgccggcgtt tgaccgtaac
     5461 ggcaagtccg ccggtatctg gctgaacccg ctgaccacgg atgacggaaa cgggctgcgg
     5521 ggattcagcg gtgaaggtcg ggtaaaaggc agcggggatg cgcagttcgt ggccctgcag
     5581 ggcagccgta acggagaaag cctgctggct gataatatgc aggatggcgt ccggattgcc
     5641 cgtgataatc ctgacagtgg tgtggtggta agaatcgccg gtgaaggccg tccgtggaat
     5701 cccggtgcca tcaccggtgg ccgcgtgtgg ggggatatcc cggacaacag cgtccagccg
     5761 ggagccggaa atggtgaacc ggtcacggca gaggtactgg cacagcggca ggctgaagag
     5821 gccatccgcc gtgaaacgga acgtcgcgca gatgaaattg tccgtaaaat ggcagagaac
     5881 aaacctgacc tgccggacgg caaaacagag caggctgtca gggaaattgc cgggcaggag
     5941 cgtgaccggg ctgccataac tgaacgggaa gccgcgctgc cggagagtgt actgcgtgaa
     6001 ccacaacggg tgcgggaagc ggtccgggaa gttgcccggg aaaatttgct gcaggagcgt
     6061 ctgcagcaga tggagcggga tatggttcgt gacctgcaga aagagaaaac cccgggcgga
     6121 gactgataca ggaagacaaa cactgatgac aacggataac acgaacacga cacgtaacga
     6181 ttcactggct gcacggaccg atacctggtt gcagtcattt ctggtctggt cacccggaca
     6241 gcgggatatc atcaaaacgg tggcactggt gctgatggta ctggaccaca tcaatctgat
     6301 attccagctg aagcaggaat ggatgtttct ggccgggcga ggagcctttc cgctttttgc
     6361 cctggtgtgg gggctgaatc tgtcccgtca tgcgcatatc cggcaaccgg ccattaaccg
     6421 gctgtgggga tggggaatta ttgcccagtt cgcatattat ctggcaggct ttccttggta
     6481 tgaggggaat atcctgtttg cctttgcagt ggcggcacag gtgctgacgt ggtgtgaaac
     6541 gcgcagtggg tggcgtacag cagcggccat actcctgatg gccctgtggg ggcctttgtc
     6601 cggcaccagt tacggtattg ccgggttgct gatgctggca gtcagcaacc ggctgtaccg
     6661 ggcggaagac agagcggaac gtctggcgct ggtcgcctgc ctgctggctg ttattccggc
     6721 gcttaacctt gccaccagtg atgcagcggc ggtggccggt cttgtgatga cggtgctgac
     6781 cgttggtctg gtgttgtgtg ccggaaaatc attaccccgt ttctggcccg gagacttttt
     6841 cccgacgttc tatgcctgtc atctggctgt gctgggcgtt ctggcgctgt gacgggtgtg
     6901 gtatctttgg ccgcaagagg atgattcgtc agaggcagaa cacagcatga cagagcagaa
     6961 gcgaccggta ctgacactga agcggaagac agagggaaca gcgcctgtcc gcagccggaa
     7021 aaccatcatc aatgtcacca cgccaccaaa gtggaaggtg aaaaagcaga agctggccga
     7081 gaaagcggcc cgtgaagcag agctggcggc aaaaaaagcg caggccagac aggcgctgtc
     7141 catttatctg aacctgccca cgctggatga ggctgtgaat accctgaagc cctggtggcc
     7201 gggattattt gacggtgaca cgccccggct tctggcctgc ggtatccggg acgtgttact
     7261 ggaagacgtg gcgcagcgga atatcccgct ctcgcataaa aaactgcgca gggcgctgaa
     7321 ggccatcacc cgttcagaaa gctatctgtg tgccatgaaa gccggtgcct gccggtatga
     7381 cacggaaggg tatgtgacgg agcatatttc tcaggaggag gaagcgtatg cgggagcgcg
     7441 tctggcgaaa attcgtcgcc agaaccggat aaaggcagaa cttcaggccg tgcttgatga
     7501 gaaataaaaa aagcctcccc cagagagagg aggcagggaa ataaggctta aaaggaaata
     7561 acttccgctt ccgaaaggaa gccagtccac tgaattattt atcacaataa tactgacaac
     7621 taaaggggga caagaaaatg aatggattca gaaacagttc acggaacggc caggtctggc
     7681 gttaccagcg tgccggcggg cgggccgtta ttctggaagt cagtggacgc tggatggaag
     7741 cggcagaagc atggcgacgg gctgcctgtg ttgcgcctag gacagactgg cagcagtttg
     7801 cccggaaaag ggctgaacac tgccaccggc gctgccgggg aagggtgtaa cgaaaaaagc
     7861 agccttcatt actctaggag tcagaaggct gaaaggagtt tatgagattt gcttcttaat
     7921 atccggtcgg gcagtatgac cggacagttc tgagtagcac agagtctttt ccgcattaag
     7981 tcgatttttt atattttgtg attagtttca caaaataagg tgttattcaa tgtgtgctgc
     8041 actattcagg ggggctgaaa tacaggtggc atttatctga tatggagaat aacatgagga
     8101 aatatattcc actggcatta tttatctttt catggccggt attgtctgca gatattcatg
     8161 gacgggttgt tcgtgtactg gacggtgata ccatcgaggt catggactca ctgaaggcgg
     8221 tgcggatcag gctggtaaat attgatgcac cggaaaagaa acaggattat ggccggtggt
     8281 caacagacat gatgaaatca ctggtcgcag gaaaaacggt taccgtcaca
//
LOCUS       DADLTY010000177         8288 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.178, whole genome shotgun sequence.
ACCESSION   DADLTY010000177 DADLTY010000000
VERSION     DADLTY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.178"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            122..2656
                     /gene="mrcB"
                     /locus_tag="JN334_003490"
     CDS             122..2656
                     /gene="mrcB"
                     /locus_tag="JN334_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HAY7439515.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2876..5119
                     /gene="fhuA"
                     /locus_tag="JN334_003491"
     CDS             2876..5119
                     /gene="fhuA"
                     /locus_tag="JN334_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HAY7439516.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            5170..5967
                     /gene="fhuC"
                     /locus_tag="JN334_003492"
     CDS             5170..5967
                     /gene="fhuC"
                     /locus_tag="JN334_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HAY7439517.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5967..6857
                     /gene="fhuD"
                     /locus_tag="JN334_003493"
     CDS             5967..6857
                     /gene="fhuD"
                     /locus_tag="JN334_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HAY7439518.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6854..>8288
                     /locus_tag="JN334_003494"
     CDS             6854..>8288
                     /locus_tag="JN334_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAY7439519.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHR"
BASE COUNT         1784 a         2086 c         2421 g         1997 t
ORIGIN      
        1 accctctccc tcaagggaga ggggaccgat cgagcgcaaa ttttgagaga tatcttcttc
       61 tgtcttgtaa cagaagaaca gaaaatcggg cttttgcgcc tgaatattgc ggagaaaaag
      121 catggccggg aatgaccgcg agccaattgg acgcaaaggg aaaccgacgc gtccggtcaa
      181 acaaaaggta agccgtcgtc gttacgaaga tgacgatgat tacgacgatt atgatgacta
      241 tgaggatgaa gaaccgatgc cgcgcaaagg taagggcaaa ggcaaagggc gtaagcctcg
      301 tggcaaacgc ggctggctat ggctactgct aaaactggct atcgtttttg ccgtgctgat
      361 cgccatttac ggcgtttatc tcgatcaaaa aattcgtagc cgtattgatg gcaaggtctg
      421 gcaactgccc gcggcagttt atggccgaat ggtcaacctt gaaccagata tgaccatcag
      481 caagaacgaa atggtgaagc tgctggaggc aacacagtat cgtcaggtgt cgaaaatgac
      541 ccgtcctggc gaatttaccg tgcaggccaa cagcattgag atgatccgcc gtccgtttga
      601 tttcccggac agtaaagaag ggcaggtgcg tgcgcgtctg acctttgatg gtgatcatct
      661 ggcgaccatc gtcaatatgg agaacaaccg tcagttcggt ttcttccgtc ttgatccgcg
      721 tctgatcacc atgatctcct cgccaaacgg tgagcagcgt ttgtttgtgc cgcgcagtgg
      781 tttccctgat ttgctggtgg atactttgct ggcgacagaa gaccgtcatt tttacgagca
      841 tgatggaatc agtctctact ccattgggcg tgcggtgctg gcaaacctga ccgcaggtcg
      901 cacggtgcag ggtgcgagta cgctgacgca acagctggtg aaaaacctgt tcctctccag
      961 cgagcgttct tactggcgta aagcgaacga agcttacatg gcgctgatca tggacgcgcg
     1021 ttacagcaaa gaccgtattc ttgagctgta tatgaacgag gtgtatctcg gtcagagcgg
     1081 cgacaacgaa atccgcggct tcccgctggc aagcttgtat tactttggtc gcccggtaga
     1141 agagctaagc ctcgaccagc aggcgctgtt agtcggtatg gtgaaagggg cgtccatcta
     1201 caacccgtgg cgtaacccga aactggcgct ggagcgacgt aatctggtgc tgcgtctgct
     1261 gcaacagcaa cagattattg atcaagaact ctatgacatg ttgagtgccc gtccgctggg
     1321 ggttcagccg cgtggtgggg tgatctctcc tcagccagcc tttatgcagc tggtgcgtca
     1381 ggagttgcag gcaaaactgg gcgataaggt aaaagatctc tccggcgtga agatcttcac
     1441 tacctttgac tcggtggccc aggacgcggc agaaaaagcc gccgtggaag gcattccggc
     1501 actgaagaaa cagcgtaagt tgagcgatct tgaaactgcg attgtggtcg tcgaccgctt
     1561 tagtggtgaa gttcgtgcga tggtcggagg ttctgagccg cagtttgcgg gctacaaccg
     1621 tgcgatgcag gcgcgtcgtt cgattggttc ccttgcaaaa ccagcgactt atctgacggc
     1681 tttaagccag ccgaaaatct atcgcctgaa cacgtggatt gccgatgcgc caatcgcgct
     1741 gcgtcagccg aatggccagg tctggtcacc gcagaacgat gaccgtcgtt atagcgaaag
     1801 cggcagagtg atgctggtgg atgcgttgac acgttcgatg aacgtgccga cggtaaatct
     1861 ggggatggcg ctggggctgc ctgcggttac ggagacctgg atcaaactgg gcgtaccgaa
     1921 agatcagttg catccggttc cggcaatgct gctgggggcg ttgaacttaa cgccaatcga
     1981 agtggcgcag gcattccaga ccatcgccag cggtggtaac cgtgcaccgc tttctgcact
     2041 gcgttcggta atcgcggaag atggtaaggt gctgtatcag agcttcccgc aggcagaacg
     2101 cgctgttccg gcgcaggcgg cgtatctgac actatggacc atgcagcagg tggtacaacg
     2161 tggtacgggt cgtcagcttg gggcgaaata cccgaacctg catctggcag ggaaaacagg
     2221 gactaccaac aataacgtag atacctggtt tgcaggcatt gacggcagca cggtgaccat
     2281 cacctgggta ggccgtgata acaaccagcc gaccaaactg tatggagcca gcggggcaat
     2341 gtcgatttat cagcgttatc tggctaacca gacgccaacg ccgctgaatc ttgttccgcc
     2401 agaagatatt gcagatatgg gcgtggacta cgacggcaac tttgtttgca gcggtggcat
     2461 gcgtgtcttg ccggtctgga ccagcgatcc gcaatcgctg tgccagcaga gcgagatgca
     2521 gcagcagccg tcaggcaatc cgtttgatca gtcttctcag ccgcagcaac agccgcaaca
     2581 gcaacctgct cagcaagagc agaaagacag cgacggtgta gccggttgga tcaaggatat
     2641 gtttggtagt aattaacatc taagcgtgaa ataccggatg gcgagttgcc atccggtaaa
     2701 ataacatccc atctaagata ttaacccttt cttttcatct ggttgtttat taacccttca
     2761 ggaacgctca gattgcgtac cgcttgcgaa cccgccagcg tttcgaatat tatcttatct
     2821 ttataataat cattctcgtt tacgttatta ttcactttac atcagagata taccaatggc
     2881 gcgttccaaa actgctcagc caaaacactc actgcgtaaa atcgcagttg tagtagccac
     2941 agcggttagc ggcatgtctg tttatgcaca ggcagcggtt gaaccgaaag aagacactat
     3001 caccgttacc gctgcacctg cgccgcaaga aagcgcatgg gggcctgctg caactattgc
     3061 ggcgcggcag tcagctaccg gcactaaaac cgatacgccg attcaaaaag tgccacagtc
     3121 tatttctgtt gtgaccgccg aagagatggc gctgcatcag ccgaagtcgg taaaagaagc
     3181 gcttagctac acgccgggtg tctctgttgg tacgcgtggc gcatccaaca cctatgacca
     3241 cctgatcatt cgcggttttg cggcagaagg ccaaagccaa aataactatc tgaatggcct
     3301 gaagttgcag ggcaacttct ataacgatgc ggtcattgac ccgtatatgc tggaacgcgc
     3361 tgaaattatg cgtggcccgg tttccgtgct ttacggtaaa agcagtcctg gcggtctgtt
     3421 gaatatggtc agcaagcgtc cgaccaccga accgctgaaa gaagttcagt ttaaagccgg
     3481 tactgacagc ctgttccaga ctggttttga ctttagcgat gcgttggatg atgacggtgt
     3541 ttactcttat cgcctgaccg gtcttgcgcg ttctgccaat gcccagcaga aagggtcaga
     3601 agagcagcgt tatgctattg caccggcgtt cacctggcgt ccggatgata aaaccaattt
     3661 caccttcctt tcttacttcc agaacgagcc ggaaaccggt tattacggct ggttgccgaa
     3721 agagggaacc gttgagccgc tgccgaacgg taagcgtctg ccgacagact ttaatgaagg
     3781 ggcgaagaac aacacctatt ctcgtaatga gaagatggtc ggctacagct tcgatcacga
     3841 atttaacgac acctttactg tgcgtcagaa cctgcgcttt gctgaaaaca aaacctcgca
     3901 aaacagcgtt tatggttacg gcgtctgctc cgatccggcg aatgcttaca gcaaacagtg
     3961 tgcggcatta gcgccagcgg ataaaggcca ttatctggca cgtaaatacg tcgttgatga
     4021 tgagaagctg caaaacttct ccgttgatac ccagttgcag agcaagtttg ccactggcga
     4081 tatcgaccac accctgctga ccggtgtcga ctttatgcgt atgcgtaatg acatcaacgc
     4141 ctggtttggt tacgacgact ctgtgccact gttcaatctg tacaatccgg tgaatcccga
     4201 tttcgacttc aatgccaaag atccggcaaa ctccggccct taccgcattc tgaataaaca
     4261 gaaacaaacg ggcgtttatg ttcaggatca ggcgcagtgg gataaagtgc tggtcaccct
     4321 aggcggtcgt tatgactggg cagatcaaga atctcttaac cgcgttgctg ggacgaccga
     4381 taaacgtgat gacaaacagt ttacctggcg tggtggtgtt aactacctgt ttgataatgg
     4441 tgtaacacct tacttcagct atagcgaatc gtttgaacct tcttcgcaag ttgggaagga
     4501 tggtaatatt ttcgcaccgt ctaaaggtaa gcagtatgaa gtcggcgtga aatatgtacc
     4561 ggaagatcgt ccgattgtag ttactggtgc cgtgtataat ctcactaaaa ccaacaacct
     4621 gatggcggac cctgagggtt ccttcttctc ggttgaaggt ggcgagatcc gcgcacgtgg
     4681 cgtagaaatc gaagcgaaag cggcgctgtc ggcgagtgtt aacgtagtcg gttcttatac
     4741 ttacaccgat gcggaataca ccaccgatac tacctataaa ggcaatacgc ctgcacaggt
     4801 gccaaaacac atggcttcgc tgtgggctga ctataccttc tttgacggtc cgctttcagg
     4861 tctgacgctg ggcaccggtg gtcgttatac tggctccagc tatggtgatc cggctaactc
     4921 ctttaaagtg ggaagttata cggtcgtgga tgcgttagtg cgttatgatc tggcgcgagt
     4981 cggcatggcg ggctccaacg tggcgctgca tgttaacaac ctgttcgatc gtgaatacgt
     5041 cgccagctgc tttaacactt atggctgctt ctggggcgca gaacgtcagg tcgttgcaac
     5101 cgcaaccttt cgtttctaat ttctcttttg gggcacggat ttccgtgccc atttcacaag
     5161 ttggctgtta tgcaggaata cacgaatcat tccgatacca cttttgcact gcgtaatatc
     5221 tcctttcgtg tgcccgggcg cacgcttttg catccgctgt cgttaacctt tcctgccggg
     5281 aaagtgaccg gtctgattgg tcacaacggt tctggtaaat ccactctgct caaaatgctt
     5341 ggtcgtcatc agcctccgtc agaaggggag attcttcttg atgcccagcc gctggaaagc
     5401 tggagcagca aagcgtttgc ccgcaaagtg gcttatttgc cgcagcagct tcctccggca
     5461 gaagggatga ccgtgcgtga actggtggcg attggtcgtt acccgtggca tggcgcgctg
     5521 gggcgctttg gtgcggcaga tcgcgaaaag gtcgaggaag ctatctcgct ggttggctta
     5581 aaaccgctgg cgcatcggct ggtcgatagt ctctctggcg gcgaacgtca gcgggcgtgg
     5641 atcgccatgc tggtggcgca ggatagccgt tgtctgttgc tcgacgaacc gacttcagcg
     5701 ctggatatcg cccaccaggt tgatgtgctg gcgctggtgc accgtttaag tcaggaacgt
     5761 ggcctgacgg tcattgccgt gttgcacgat atcaatatgg cggcacgcta ctgtgattat
     5821 ctggtcgccc tgcgcagtgg tgaaatgatt gctcagggaa cgcctgcgga aattatgcgc
     5881 ggcgaaaccc tcgaaatgat ttatggcatc ccgatgggta ttttgccgca tccggcgggt
     5941 gctgcacctg tgagttttgt ttattgatga gcggcttacc tcttatttcg cgccgtcgac
     6001 tgttaacggc gatggcgctt tctccgttgt tatggcagat gaataccgcc cacgcggcgg
     6061 ctattgaccc caatcgtatt gtggcgctgg agtggttgcc ggtggaatta ctgctggcgc
     6121 tcggcatcgt gccttacggc gtggcggata ccatcaacta tcgcctgtgg gtcagcgaac
     6181 caccattgcc ggactcagtg attgacgtcg gtttgcgtac agaacctaac cttgaactgc
     6241 tgaccgaaat gaaaccgtcg tttatggtct ggtcggcagg atatggccct tcatcagaaa
     6301 tgctggcacg tattgcgccg ggtcgcggat ttaacttcag tgacggcaaa catccgttgg
     6361 cgatggcgcg taaatcgctg acggaaatgg cagatttact taacctgcaa agcgcagcgg
     6421 aaacgcattt agcgcaatac gaagacttta tccgcagcat gaaaccccgc tttgtgaagc
     6481 gtggtgcacg cccgttactg ctgacgacgc ttatcgatcc gcgccatatg ctggtcttcg
     6541 gtccaaacag cttgttccag gaaattctcg atgagtacgg catcccaaat gcctggcaag
     6601 gggaaaccaa cttctggggc agtaccgccg tcagtatcga tcgtctggca gcgtataaag
     6661 acgttgatgt gctctgtttt gatcacgaca acagcaaaga catggatgcg ctaatggcaa
     6721 ccccgctgtg gcaggccatg ccgtttgtcc gtacagggcg ctttcagcgc gtacccgcag
     6781 tctggtttta tggtgcaacg ctttcggcaa tgcactttgt gcgtgttctg gataacgcca
     6841 tcggaggtaa agcgtgagta aacgaattgc gcttttcccg gcgttattgc tggcgctgtt
     6901 agtgattgtc gctacggcgc tcacctggat gaacttctca caggcgctgc cgcgcagtca
     6961 gtgggcgcag gccgcctggt cgccggatat tgacgtcatc gagcagatga tttttcacta
     7021 cagcttgttg ccgcgtctgg cgatttcgct gctggtgggc gcgggcctgg ggctggtggg
     7081 cgtgctgttt cagcaagtac tgcgtaaccc gctggcggaa ccgacgacgt tgggcgttgc
     7141 aacaggcgcg caactgggga ttaccgtcac cacgctctgg gcgatcccta gcgcgatggc
     7201 gagccagttt gctgcgctgg caggggcttg cgttgttggc ttaattgtct ttggcgtcgc
     7261 gtgggggaaa cggctgtcgc cggtaacgct gattttcgcg gggttggtag tgagccttta
     7321 ttgcggcgca atcaatcagt tactggttat cttccatcat gaccagttgc aaagcatgtt
     7381 tctgtggagc actggaacgc tgacgcaaac cgactggggc ggtgttgagc gtttatggcc
     7441 gcagttgctg ggcggtgtga tgctgacgtt gctgctactt cgtccgttaa ccctgatggg
     7501 gcttgatgat ggcgtggcgc gcaatctcgg gctggccttg tcgcttgcgc gtctggcggc
     7561 gctgtcgctg gcgattgtca tcagtgcgct gctggtgaac gctgtgggga ttatcggctt
     7621 tatcgggttg ttcgcgccgc tgctggcaaa aatgctgggg gcgcggcgtc tgctgccacg
     7681 actgatgctg gcgtcgttga ttggtgcgct gatcctctgg ctttccgatc aaatcatcct
     7741 ctggctgact cgcgtgtgga tggaagtgtc caccggttcg gtcactgcgt tgatcggtgc
     7801 gccgctgcta ctgtggctgc tgccgcgttt acgcagcatt agcgcgccgg atatgaaggt
     7861 taacgatcgt gtcgcgactg aacgccaaca tgtgctggcg tttgccctcg cgggcggcgt
     7921 gctgttgttg atggctgtgg tggtggcgct gtcgtttggt cgtgatgcgc acggctggac
     7981 gtgggcgagc ggggcgttgc tcgaggattt aatgccctgg cgctggccgc gaattatggc
     8041 ggcgctgttt gcgggcgtca tgctggcggt agcgggctgt attattcagc gactgaccgg
     8101 caacccgatg gcaagcccgg aagtgctggg gattagctcc ggcgcggcgt ttggtgtggt
     8161 gttaatgctg tttctggtac cgggtaatgc ctttggctgg ttgttacccg caggcagtct
     8221 cggggcggcg gtgacgctgt tgatcattat gatcgccgcc ggacgcggtg gattttcccc
     8281 acaccgca
//
LOCUS       DADLTY010000178         8283 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.179, whole genome shotgun sequence.
ACCESSION   DADLTY010000178 DADLTY010000000
VERSION     DADLTY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8283
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.179"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            4..435
                     /locus_tag="JN334_003495"
     CDS             4..435
                     /locus_tag="JN334_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439520.1"
                     /translation="MTMNLHTPTTAPKAAPATSVSPLEQSGSPKTKFPEIKTVKTAVV
                     KLISDIEGNLDQRLVYREAVEGLCQEAANRITQCMHSRSTGQTLRDLRELLSVTLDTI
                     IVVEFEGEQTRLNYGGRSAEDVIAMLKALHLEGRKAWLEVE"
     gene            complement(467..994)
                     /locus_tag="JN334_003496"
     CDS             complement(467..994)
                     /locus_tag="JN334_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HAY7439521.1"
                     /translation="MRKTTLLVLAAALCVPAFATAAPVLTAGFSPSDGRPALEIVLGA
                     INNARQSIDVAAYSFTSKPVATALAGANRRGVAVRVVADEKANSDRYTAVTYLINQGV
                     PVHLNGRYAIMHNKFMVIDGKNVQTGSFNYTASAVSRNAENVLLIEDAPQLAEAYQRE
                     FNRLWDEGIPLNALY"
     gene            complement(987..1733)
                     /locus_tag="JN334_003497"
     CDS             complement(987..1733)
                     /locus_tag="JN334_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HAY7439522.1"
                     /translation="MLISSYQERYARSTEKIRVLLNFLKEETYSDFQIIKLLFDFKSD
                     RPLYRLLEKVEKMGLIQKHVHESRASRISLWGITTDGLAVILTADDNIMPARFEPYRL
                     TGWSLDHHLDNQLVRLTLEKNGATGWINGDRSTFLSQYSVKHRPDGLISLPDGRLIAV
                     ETERRIKTKARYQSIMASHLLAISDKKWRYAFYVAPDEAKKRALKIIFDSIASVIVKN
                     QHVQLEAKHRDVFRFYTLDELKNLELDNYA"
     gene            complement(1748..3655)
                     /locus_tag="JN334_003498"
     CDS             complement(1748..3655)
                     /locus_tag="JN334_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAY7439523.1"
                     /translation="MIFDKLNHLLSPIANAVLAFIAFLQAHQLALALLSGVMLPFIFS
                     AKHGGQEKPTFLQRILILLSMICFVFGSIAPVAVWFLQRIYGREDIASPPLLTWTMAL
                     LFTVAGFILHVVLRRLISPEFDKVKCGMVKKTSMERDRRTDVRRVKEILPVTTEYDPM
                     DYIDLNKGIFIGLSREGEPQYIPLKEWQTQHADIIGTTGAGKGVVTGILLYQSILADE
                     GVFVLDPKNDEWAPHLYKKACADAGKPFVLIDLNKQEYQMNLIDGITADHLEELFVAG
                     FSLAEKGEAADFYRIDDRKAARTAAQFVKDNPGATVRDIYNGDYVQGIGETIKAFHGK
                     TEELAMLNSINAPGGFALKEIFDKGGCCYIIGSMRNSKIITAQRMILVRLFQLAELRD
                     RVAGNIRPIAIFKDEVKYHLSKPALEGLGAARDKGVHILMAHQSIADLRDCPADLNGD
                     AVVGAVVENCKFKLVYKLQDPDTAEWIARMSGTILVDDESRKVTTTAVLTEKVDNERM
                     LRQAESYYIDSNMLQNLPKFVSYIFSQNALAFPSLISPLKVKKAPLELFTLPPVAVAT
                     VAEVKPVLDLDFDKTAPAVPAPDIIPVSATTTKKPSATPIPTPPVQPDEEWLMASQPA
                     PNDEIHSLLDL"
     gene            complement(4364..4756)
                     /locus_tag="JN334_003499"
     CDS             complement(4364..4756)
                     /locus_tag="JN334_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HAY7439524.1"
                     /translation="MNRIQDELTSERALKKFLRESDFAFLEMLSARISAALDEKRAEH
                     IEQEKLRRQREEKRNELLALIESEGFSPTELVSTTPAKKSAKRKAKYQYFENGVHKKW
                     SGVGRVPVVIQQELDAGKPLESFLITTD"
     gene            complement(4753..6954)
                     /locus_tag="JN334_003500"
     CDS             complement(4753..6954)
                     /locus_tag="JN334_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAY7439525.1"
                     /translation="MRLWIAEKPAVASDIAKALGGNFTRHDGYAESATDIVSYCVGHV
                     LEMVPPETINPAYATWSLDTLPLKLYPVQLQPKASVAKQANTLVKLIRRPDIKMVVHC
                     GDPDDEGQLLVDEVLEFAGNTAPVKRALINDNTAPAVKKAINSLRDNSDFRGMYLKAL
                     MRSAGDAIFGFSMSRCYTLKARDKGYKGTISVGRVQTPVLGMIVRRWRDNQAHSEAFY
                     YQLAGQFISGTDVVCARWQTSEYAPVDDKKRLTDKAWGEGLARALAGKPASVLAAATD
                     RGKTTAAPLPFNLLRLQVYMNRRHKLTAQQTLDITQKLKDSKYITYNRSDCSYLSDEQ
                     FNDAPQVVAALKTLDVFNGLTTDTAQKSAAFDSKKVTAHTAIIPTTNMPDLSRLTDKE
                     KAVYLTIAQFYLAQFVAKKRYDESIAEIKCGDEMFKVSARKITDAGFTTFLNDAEEDD
                     EEDENSTSFEAISRLQTGATLTCREVVISEKKTKPLPLFTEATLLAALVRVADFVADP
                     RIKKLLKEKDKDKKDEHGGIGTPATRAAIIETLKTRDYITVEKGKFIPTAAGLALIDA
                     LPDSVTQPDMTAVWAEKQAAVETGEMTIERFIDGLYAEVSRLVESADIAISATEKHPV
                     KTALNRLAVKCPSCGSELVMTPKTCACTGCQFKIWLEVRGKKLTEKQAEKLIGKGKTD
                     VIKGFKSKKNDDTYSMIVTLKNAQTGELGFEYPPRDPLKGLNVSMPKELKL"
     gene            complement(6951..7259)
                     /locus_tag="JN334_003501"
     CDS             complement(6951..7259)
                     /locus_tag="JN334_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439526.1"
                     /translation="MATKTGFIAPPWPHVRGDSVSAASLISRIEKEAHRGCGLHYEIY
                     QYRALCELHDVLTSLSVRDAETFMTVAADHGFSLSDNDFQESRMMYFETMDEIRRDQE
                     "
     gene            complement(7235..7570)
                     /locus_tag="JN334_003502"
     CDS             complement(7235..7570)
                     /locus_tag="JN334_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439527.1"
                     /translation="MINQTVSINNFSGLLKDEELPAGDLQTVLLVLSEISQKQDHFQA
                     NTSELVQLAAQHSPTFQALPAERQSFFRSVLSMPVFFYTVPLSDDEAAEWEKLNDKDA
                     LQWQQKPDL"
     gene            complement(7597..7914)
                     /locus_tag="JN334_003503"
     CDS             complement(7597..7914)
                     /locus_tag="JN334_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439528.1"
                     /translation="MKHTVITAALLCALTSPAFAADGQNADPNDPCAMVLCLAGKLDG
                     SSPAECDPMYKNFMSIKKKKKGSFLPDHTAAARQKKLSACPSADSAIVGKIISKFGRM
                     NGW"
     gene            complement(8005..>8283)
                     /locus_tag="JN334_003504"
     CDS             complement(8005..>8283)
                     /locus_tag="JN334_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439529.1"
                     /translation="PVWEPNRLIVASPVTEGHWSQVLTRFEPGGIYPAAQWYAVAHAS
                     RAVVNAPDGQRFFAAKTWLREGGYKGVVEFRPRSGCLTCDTSEIVFYR"
BASE COUNT         1726 a         2301 c         2271 g         1985 t
ORIGIN      
        1 accatgacga tgaacctgca caccccgaca accgccccta aagctgcacc ggcgaccagc
       61 gtatccccgc tggagcagtc aggctcccca aaaacgaaat ttcctgaaat caaaaccgtc
      121 aaaacggcgg tggttaaact gatcagcgac atcgagggca acctcgatca gcgtctggta
      181 tatcgcgagg ccgtagaggg attgtgccag gaggcggcca accggataac ccagtgcatg
      241 cattcacgca gtacagggca gacgctacgt gatctccgcg agctgctgtc ggtgacgctg
      301 gacaccatta tcgtggtgga gtttgaaggg gagcagaccc gtctcaacta tggcggacgt
      361 tcagcagaag acgtgatcgc catgctgaaa gcgctgcatc ttgaaggacg caaagcctgg
      421 ctggaagtgg agtaaaaaaa aagccccgtc cgcagacggg gctgattcag tacagggcat
      481 tcagcgggat gccctcatcc cacaggcggt taaattctcg ctggtacgcc tccgccagct
      541 gcggcgcatc ctcgatcagc agcacgtttt ccgcgttgcg cgaaacggcg cttgccgtgt
      601 agttaaacga cccggtctgg acgtttttgc cgtcgatcac cataaacttg ttgtgcatga
      661 tggcgtaacg gccgttaagg tgcaccggca cgccctggtt tatcagatac gtcacggcgg
      721 tgtagcggtc gctgttcgcc ttttcgtcgg ccaccacgcg cacggccacg ccgcgccggt
      781 tagcaccggc gagcgccgtc gcgaccggct tactggtgaa gctgtaggcc gcgacgtcaa
      841 tgctctgcct ggcgttgttt atggcgccga gcacgatttc aagagcaggg cgcccgtcag
      901 acggcgaaaa acccgccgtc aggacgggcg cggcggtggc gaacgcgggc acgcagaggg
      961 cggcggccag caccagaagg gtggttttac gcatagttgt ccagctccag gtttttcagt
     1021 tcatcgaggg tgtaaaagcg gaaaacgtcg cggtgttttg cctccagttg cacatgctgg
     1081 tttttcacga tcacgctggc gatgctgtcg aaaataatct tcagggcgcg ctttttcgcc
     1141 tcgtccgggg cgacgtaaaa cgcgtaccgc cattttttat cgctgatagc cagcaggtgg
     1201 ctcgccatga tggactgata gcgcgccttt gttttgatac gtcgttcggt ttcaacggcg
     1261 atcagcctgc cgtccggcag gctgataagc ccgtcgggac ggtgtttgac gctgtactga
     1321 ctgaggaacg ttgaacggtc gccgttaatc caccctgtcg cgccgttctt ctccagcgta
     1381 aggcgcacta gctggttatc aaggtgatgg tcgagtgacc agccggtcag gcggtagggt
     1441 tcaaagcggg cgggcattat gttgtcgtcc gccgtgagga taacggccag cccgtcggtg
     1501 gtaatgcccc acagggatat tctcgacgcg cgggattcat gcacgtgctt ctgaatcagc
     1561 cccattttct ccaccttctc caacaaccgg tagagagggc ggtcagattt aaaatcaaaa
     1621 agcagcttta tgatttgaaa atcgctgtag gtttcctcct tgagaaaatt cagcagtacg
     1681 cgaatttttt cagtgctgcg tgcataacgt tcctgatacg acgaaataag catagaaact
     1741 ccttatttta taaatccagc aacgaatgta tttcgtcatt tggagcgggc tgtgatgcca
     1801 tcagccactc ctcgtcaggt tgcaccggcg gagtgggtat tggcgttgct gagggttttt
     1861 tggtggttgt tgcgctgacc ggaataatgt caggtgccgg aactgccgga gccgttttat
     1921 caaaatccag atccagcacc ggtttaactt ctgccaccgt agcgactgcg accggcggca
     1981 gggtaaacag ctccagtggg gcttttttaa ccttgagcgg tgagattaac gacgggaacg
     2041 cgagcgcgtt ctggctgaag atatagctga cgaacttcgg caggttctgg agcatattgc
     2101 tgtcgatgta atagctttcc gcctgccgaa gcatgcgctc gttatcgact ttttctgtca
     2161 gcaccgccgt ggtggtgact ttccggcttt catcatcgac taaaatcgtg cccgacatgc
     2221 gcgcaatcca ttcggcggtg tctggatcct gaagtttata taccagctta aacttgcagt
     2281 tttccacaac cgccccaacc acggcgtcgc cgtttaaatc cgccggacag tcgcgcaggt
     2341 cggcaattga ctggtgcgcc attaatatat ggacgccttt atcgcgtgcc gcgcctaacc
     2401 cttcgagcgc cggtttggac aggtgatatt tcacctcgtc cttaaagatg gcgatggggc
     2461 ggatattccc cgcgacgcgg tcgcgcaatt ccgcgagctg gaacaggcga actaaaatca
     2521 tgcgctgcgc ggtaatgatt ttagagttac gcatcgaacc gatgatatag cagcagccgc
     2581 ccttgtcgaa aatctccttc agcgcaaaac cgcctggcgc gttaatgctg ttgagcattg
     2641 ccagctcctc ggttttaccg tgaaaggctt taatcgtctc gccaatgccc tgaacataat
     2701 cgccgttata aatatcccgc acggttgcgc ccggattatc cttcacgaac tgcgccgccg
     2761 ttctcgccgc cttgcggtcg tcgatacggt aaaaatccgc cgcctcgcct ttttccgcca
     2821 gactgaaacc ggccacgaat aattcctcca gatggtcggc ggtgataccg tcgataaggt
     2881 tcatctggta ttcctgttta ttcaggtcga taagcacaaa gggttttccg gcgtccgcgc
     2941 acgctttttt atacaggtgc ggtgcccact cgtcgttttt ggggtcgaga acaaaaacgc
     3001 cctcgtcggc gaggatgctc tgataaagca gaataccggt tacgacgcct ttaccggcac
     3061 cagtggtgcc gataatgtcg gcgtgctggg tctgccattc ttttaacgga atatattgcg
     3121 gctcaccctc gcggctcagc ccgataaaaa tccccttatt cagatcgatg taatccatcg
     3181 gatcgtattc tgtggtgacg ggtaaaattt cttttacccg gcgaacgtcg gttctgcggt
     3241 cgcgctccat gctggttttt ttcaccatgc cgcatttgac cttatcaaat tcaggcgaga
     3301 tcaaacgccg taaaaccacg tgcagaataa aaccggcgac ggtaaacagt agcgccattg
     3361 tccaggtaag cagcggcgga ctggcgatat cttcccggcc ataaatgcgc tgtaaaaacc
     3421 agacggccac tggcgctatc gagccgaaaa caaaacagat catggaaagc agaattaaga
     3481 tcctttgcag aaacgtcggc ttttcctgac cgccgtgttt cgcagaaaat ataaaaggca
     3541 gcatgacgcc ggacagcagc gctaacgcca gttgatgcgc ctgcaaaaag gcgataaagg
     3601 ccaggacggc atttgctatc ggcgacagca aatgattcag tttgtcgaat atcacgatat
     3661 tctccttcac ttaaattcgg tcagcaccat cggtcagtca gaacggtcac aggttgtgat
     3721 cgttctgact gaccgattcc atgctgcaaa agaagtggcc gctaagcggc catgatcgca
     3781 ggaggtacac tcccgcgatc taacggcgtg cggcattacg cgcgggctgg acgcccttac
     3841 tgcatgtcgc atgggggtaa tgtcctggct gaacgcccgg ccaaaacccc caaggtcaaa
     3901 agaggccaca ccgtcgcgcg ctacgcgcga ccacccccta aaagacgcct gttttagccc
     3961 ttttcttcgc cttgttcgtt cctcacaaga cgaattaaag ggaaactagg ctttttaggg
     4021 ggtggtcgcg ccacgctacc ggcttttggc cggagactta ggcacgacgg tgtggggggc
     4081 aagcccccca aataaaccga cgcgtgggcg cgtctttttg ctcgctgggg cggcaaaatt
     4141 tattcggccc cccccggctt ttttcggctc acttggggtg agcgctcaaa aagtggaatc
     4201 ggcgacgcaa attagcaatt tcttcgaaat tgaatttact cgcctcctgc cacttcgcag
     4261 actcaggcgg cctgtcgatt agcaattttt tcaaaattga atcgccatgc tcgccagtct
     4321 tcgaagcccg aaaagccagg tcaaaaccga ccctgtgccg ttgttagtcg gtggtaataa
     4381 ggaaggattc aagaggtttt ccggcgtcaa gttcttgctg aattacaacc ggcacgcgtc
     4441 cgacgcctga ccatttttta tgcacgccgt tttcaaaata ctgatattta gctttgcgtt
     4501 ttgccgactt tttggcggga gttgtactaa ccagctctgt cggtgaaaag ccctccgatt
     4561 caatcagcgc cagcaattca ttgcgctttt cttcccgctg tcggcgtagc ttttcctgct
     4621 cgatatgctc tgcgcgtttt tcatcaagcg ctgctgaaat acgtgcactc agcatctcca
     4681 gaaaggcaaa atcactttca cgtaaaaact ttttcagcgc gcgctctgag gttaattcgt
     4741 cctggatacg gttcataatt tcagctcctt cggcatagat acgttcaatc ctttcagcgg
     4801 atcgcgtggt ggatattcaa aaccgagttc cccggtttgc gcgtttttca gcgtgacgat
     4861 catcgaatag gtgtcgtcgt ttttcttcga tttaaaacct ttaataacgt cggttttgcc
     4921 cttgccgatt aacttctcgg cctgtttttc cgtcagcttt ttgccgcgca cttccagcca
     4981 tattttgaac tgacagcccg tacaggcgca ggttttcggc gtcataacca gctcagaacc
     5041 gcacgacgga catttaaccg cgaggcggtt aagcgccgtt ttaacgggat gtttttccgt
     5101 cgcgctgatg gcaatatcgg cgctctcaac caggcgggaa acttccgcgt aaagaccatc
     5161 aataaagcgc tcgatggtca tttcgccggt ttcaaccgcc gcctgttttt cagcccatac
     5221 ggcggtcata tccggctgcg tcaccgagtc gggcagggca tcaattaacg ccagcccggc
     5281 ggcagttggg ataaatttgc ccttttccac ggtgatgtaa tcgcgggttt tcagcgtctc
     5341 aataatagcc gcgcgggtcg ccggtgtgcc gatgccgccg tgttcatctt ttttgtcttt
     5401 atccttctcc ttgagcaatt ttttaatgcg cggatcggcg acaaaatcgg ccacgcgaac
     5461 gagggcagcg agcagggtcg cctcggtaaa cagcggcagc ggtttagttt ttttctccga
     5521 aatcaccacc tcgcggcagg ttaatgtcgc cccggtttgc aggcggctga tggcctcaaa
     5581 cgaggtgctg ttttcgtcct cctcgtcgtc ttcttccgcg tcgttcagga acgtcgtaaa
     5641 acccgcatcg gtgattttac gcgccgagac tttaaacatc tcgtcaccgc atttaatctc
     5701 cgcgatcgac tcgtcatagc gctttttcgc cacgaactgc gcgagataaa actgcgcgat
     5761 ggtgagataa accgcctttt ccttgtcggt aaggcggctt aaatccggca tgttcgtcgt
     5821 cgggataatg gcggtgtggg cggtgacttt tttactgtca aacgccgcac ttttttgcgc
     5881 cgtgtcggtg gtaaggccgt taaaaacgtc gagcgttttt aaggccgcca ccacctgtgg
     5941 cgcgtcgttg aattgttcat cggacagata agaacagtca gagcggttgt aggtaatgta
     6001 tttactgtcc ttgagcttct gcgtgatatc gagcgtctgc tgcgcggtca gtttgtgcct
     6061 gcggttcata tacacctgga ggcgcagcag gttaaacggc agcggtgcgg ccgtggtttt
     6121 tccccggtcg gtggcggccg cgagcacgct ggccggtttt ccggccagag ccctcgcgag
     6181 tccttccccc caggccttat ccgtcagccg ctttttatcg tccaccggcg cgtattcgga
     6241 cgtctgccag cgggcgcaga caacgtccgt accgctgata aactgccctg cgagctggta
     6301 gtaaaacgcc tcgctgtgcg cctgattgtc gcgccaccgg cgcacaatca ttcccagcac
     6361 cggcgtctgg acgcggccaa ccgaaatcgt gcccttatag cccttatcgc gggctttgag
     6421 ggtgtaacag cggctcatac tgaagccaaa aatcgcgtct cctgcgctgc gcatcagggc
     6481 tttcaggtac atcccacgaa aatcgctgtt atcgcgcagg gagttgatgg cttttttcac
     6541 cgctggcgcg gtgttgtcgt taataagcgc ccgtttcacc ggcgcggtgt ttccggcgaa
     6601 ctccagcacc tcgtcaacca ggagctgccc ctcgtcgtcc gggtcgccgc agtggacaac
     6661 cattttgata tccgggcgcc gaatcagctt caccagggta ttagcctgct ttgccacgct
     6721 cgcttttggc tgaagctgta ccgggtacag cttcagcggc agggtgtcga gcgaccaggt
     6781 ggcgtaggcc gggttgatcg tctccggggg caccatttcc agcacgtggc cgacgcagta
     6841 gctcacgata tcggtcgccg attcggcata gccgtcgtgc cgggtgaagt tgccgcccag
     6901 cgctttcgcg atatcgctcg cgactgcggg tttttctgca atccacagtc tcattcttga
     6961 tctctcctga tttcatccat cgtttcgaag tacatcattc gactttcctg aaagtcgtta
     7021 tcagacagac taaagccatg atccgccgct actgtcatga acgtctcagc atcacggacg
     7081 ctgagactgg tcagaacatc gtgcaattcg cacagggcgc gatattggta gatttcgtaa
     7141 tgcaggccgc agccgcgatg cgcttctttc tcgatgcgag aaatcaggct cgcggcacta
     7201 acggaatcac cacgcacatg cggccacggc ggggctataa atccggtttt tgttgccatt
     7261 ggagtgcatc cttatcgttg agtttttccc attccgccgc ctcgtcgtcg ctcagcggaa
     7321 cggtgtaaaa gaagaccggc atactgagca cggagcggaa gaacgactga cgttctgctg
     7381 gtaatgcctg gaaggtcggc gagtgctgcg cggcgagctg gacgagttcg gacgtgttgg
     7441 cctgaaaatg atcctgtttc tggctgattt ccgacaaaac gagcagcacc gtctgcaaat
     7501 cacctgcagg aagctcctca tctttcagca gcccggaaaa gttattaata gaaacggttt
     7561 gattaatcac gtggtgtctc ctttacggct gccgttttac caaccgttca tacggccaaa
     7621 tttcgagatg atcttgccga caatggcgct gtcagcggac gggcaggcgc tcagtttttt
     7681 ctgccgcgct gcggcggtgt gatcgggtaa aaacgacccc ttttttttct ttttgatgct
     7741 catgaaattt ttgtacatcg ggtcacactc ggcgggactg ctgccgtcga gctttccggc
     7801 caggcacagc accatcgcgc acgggtcatt cgggtcggca ttctggccat cggcggcgaa
     7861 cgccggagaa gtaagggcgc acagcagcgc cgccgtgatt acggtgtgtt tcatgatgat
     7921 ttcctctgaa gggaagcagg gcgctaacgc ccgttggttg ttcaacgtcg gtgacggtct
     7981 gaaggactca gaccgtcacc gacgttatcg gtagaagacg atttctgagg tgtcgcaggt
     8041 caggcagccg gagcgggggc gaaactccac cacacccttg tagccgccct cgcggagcca
     8101 ggttttggcc gcaaagaagc gctggccgtc cggggcattt actaccgcgc gcgaggcgtg
     8161 cgcaaccgcg taccactgtg cggccgggta aatcccgccg ggttcaaaac gcgtaagcac
     8221 ctgcgaccag tgcccttccg tcaccggcga ggccacgata agacggttgg gttcccatac
     8281 cgg
//
LOCUS       DADLTY010000179         8237 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.180, whole genome shotgun sequence.
ACCESSION   DADLTY010000179 DADLTY010000000
VERSION     DADLTY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.180"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(18..1766)
                     /gene="msbA"
                     /locus_tag="JN334_003505"
     CDS             complement(18..1766)
                     /gene="msbA"
                     /locus_tag="JN334_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HAY7439530.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(1803..4062)
                     /locus_tag="JN334_003506"
                     /pseudo
     CDS             complement(1803..4062)
                     /locus_tag="JN334_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(4269..4553)
                     /gene="ihfB"
                     /locus_tag="JN334_003507"
     CDS             complement(4269..4553)
                     /gene="ihfB"
                     /locus_tag="JN334_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAY7439531.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(4713..6386)
                     /gene="rpsA"
                     /locus_tag="JN334_003508"
     CDS             complement(4713..6386)
                     /gene="rpsA"
                     /locus_tag="JN334_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAY7439532.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(6497..7180)
                     /gene="cmk"
                     /locus_tag="JN334_003509"
     CDS             complement(6497..7180)
                     /gene="cmk"
                     /locus_tag="JN334_003509"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAY7439533.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(7353..8117)
                     /locus_tag="JN334_003510"
     CDS             complement(7353..8117)
                     /locus_tag="JN334_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7439534.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         2095 a         2176 c         1936 g         2030 t
ORIGIN      
        1 agaccagatt ttttcgatca ttggccaaac tgcattttgt gaagttgcgc gtaaacgccg
       61 cggtgctcaa gcaaatcgtt atgcgtaccg cgttccacaa tgacaccatc ctcgacgacc
      121 acgatttcgt ctgccttttc aatggtagac aagcggtggg caatcaccag agaggtacgg
      181 tttttctgca actcatccag tgccgcctga atcgcacgtt cggattcggt atccagagcc
      241 gaggtagctt catccagaat cagaatcggg ctatcacgca gcaaggctcg agcgatagca
      301 atacgctgac gctgaccgcc agagagcagc acgccgtttt caccaatcac tgtatcgaga
      361 ccgttatcca tcttattgat gaagtccatg gcgtaggcca tccgcgccgc ttcttcaatt
      421 tgctcacggc tgtactgttc agtccgtgcg taagcaatgt tgttagcaac cgtatcgtta
      481 aacagatgga cattctgcga caccagagca acctggttac gtaacgacgc cagggtatac
      541 tcgcgcagat cgtgaccatc catcaggatt tcgccttcat caatatcgta aaaacgcgtg
      601 atcaggctgg cgatggttga tttacctgaa ccagagcgtc caaccagagc aaccgtcttc
      661 cctgccggaa ttttcaggtt gatgttacgc aatgcgggta cgtcacgtcc cggataagta
      721 aaggtgacat tgcggaattc cacgtcgcca gtcgcacgct cgatcacgcg cttaccttcg
      781 tctttctcct gctcactgtc cagaatggta aacagcgtct gacaagccgc cataccgcgc
      841 tggaactggg cgttaacgtt ggtcagcgat ttcagcggac gcatcagtgc aatcattgaa
      901 gagaaaacaa cggtaatcgt accggcagtc aggttatcca taacacttgg gaagctcgcc
      961 gcatacagaa caaacgccag cgccaaagag gcgatcagct gaatgatcgg atcagagatg
     1021 gaagaggctg aaaccatttt catcccctga agacgcattc ggttactgac tttatcgaag
     1081 cgtttcgttt ccacttcctg accaccgaaa atcaatactt ctttgtggcc cttcagcatt
     1141 tgttctgcac tggtggtcac ttgccccatg gtgttctgca tgtttttact gatgttgcga
     1201 aaacgcttcg atactacgcg aatcgcaatc gaaacaatcg gtgccagcac aatcaaaatg
     1261 atcgacagtt gccaactgta atagaacatc atgatgaaca ggccgatgat cgacgcacct
     1321 tcacgcacaa cagtaatcag tgcgccggaa gaagaagaag caacctgttc ggaatcgtag
     1381 gtgatacgtg acaacagcgt acccgttgac tgtttgtcaa agaatgaaac tggcattccc
     1441 atcatgtgac caaacaggcg gcgacgcatg gtcattacca cctttcctga tacccaggag
     1501 atacagtagc tggagacata gctggtgata ccacgtaaaa tcatcagtcc gatcaccacc
     1561 agcggcatcc acaccagcac ggagcgatct gttttaccaa agccatcatc aagaagtggc
     1621 ttaaggagcg ataacatgaa ggtatcgctg gctgcgttga ggattaacgc tacgcccgcc
     1681 acgatcagac ccgctttgaa aggcgcaatg gttggccaca gtcggcggaa tgtctgccac
     1741 gtagagagat ctttgtcgtt atgcattcaa aaaaccagca tttgttgaaa tagccgcata
     1801 ttctacccgt tatccactgg cacgccaaac cactgatgat accaacgagg taaaatttgc
     1861 tctctgaggc tgctaatccg ccagccttgt gctgaaaaat tgaccgtcac ttgcccctga
     1921 tgtggagtat caagccattg atatcctttc tgttgatagc gatgcttaac tttgttagag
     1981 ggcaatcgcc atgcgttata gcgcgatgcc gatgcgagtg ccacttttcc attcactcgc
     2041 tgaattaatg gcaacgatga tgaggtatta ctgccatggt gaggtacctg aagcaatgtt
     2101 gcctgcactt gctgccagta acggcttagc atcttttgtt cagctggggc ttcaatatca
     2161 ccggttagaa gaatgctatt cgtcccgtca tcaaccttaa ccacacagga atggttattt
     2221 cccttatcgt tgctaccttg taaaggccag tgcgcgctga aacgcaatcc ttgccactgc
     2281 cacgcttcgc cacgcacaca gggctgatga tgttcccagt ttaacggact tctgatccat
     2341 aacatcggcc atatatgcaa tattgaatcc agccctcccc ggtgatccag atgttcatgg
     2401 ctcagaataa cgccttccgg ttcaagatta tgccagtgga gccaggggat aatcagttgt
     2461 tgcccactat ccccttcagg ccagtcctgt gtcatagaga atcgctttgc cgtttctggc
     2521 tatcaccatt gccagccctt gcccgacatc aagcatgtac agctgccact cgtcaggtcg
     2581 aggtttttgc cacagcggcc agcacatcag caagcctcca gccacacaca ttgctggcaa
     2641 cgttcgccag gcgtttaatc gccataccac cagtaagaac catggggaaa atgatagcca
     2701 ttgccaacat tcaacaatgt tgatccaccc ttccggcaaa ctctttaacc cccagaaaag
     2761 taaagccaaa gaccggtcgg caagaaacca taacccctgt tccaggatta acggcccgct
     2821 aaaatgcaca accatcgcgg cgaggatcaa cggaaccgtg ataaatgtca ccaagggaat
     2881 tgcaaataga tttgcaataa acgaggtcag actaatgcca tgaaatatga cgatttgcat
     2941 gggcataagc aggagtgtga ttcccagttg cagatggatg agggaaacaa ctgcacgcaa
     3001 taccggcggc agttgccact caggacaggg aaaccactga taccaaaaaa tcagtgccgc
     3061 gaccgcagcg gcagagagcc ataaactttg cgagagaatg gcaacaggat ccatcagcaa
     3121 aattgccgcc agacaacata tccatacatc ccagccactc cactgtcgcc cacttaactt
     3181 aagcattccc cacgtagcaa gcgccaccac ggtacgcaat gcaggaggtt gcattcccgt
     3241 caaccaggca taaaaagcag cacagcagat tccgccaatt aatggtattt gccagtggat
     3301 ccagcgccca ggcagaaaaa tttgtccact gcgaatgagt ccggcagcca gcaacgccgc
     3361 aaaagcgatg tgcaatcccg atatcgccat taaatgcgcc gttccagtat cgcgcatgat
     3421 atttttgatt tctttgggga cggataaccg ttcccccata cctaaaccaa gaataaccgc
     3481 attccacgga tagggttgca gcgttgtttg tagtgacgcc agatactgtg cacgcaggct
     3541 acaattcggt tcaatgacac ttgcctgcag aaaacggccg gtgagcggct gatgctgggc
     3601 aatggcataa cgctggctat caaagccgcc atcattaagt tggccatgaa ctgcacgaac
     3661 tttgagtttc attgaccatt gttgtccagc acaaaccgct tgcggaagtt attcgccata
     3721 cagcacaagg cctggcgcag ggaatatacg tcgaccttgt agataagtaa tttgaccgta
     3781 atgagtggtc atgccgtcag ttgcagtgat ctcaacaatt gcatcctgcg tcgcgccagt
     3841 taaggtttct cctgcccaca aaatttgctt tgctgataat atgccccaaa cgaaaaacag
     3901 caacgtcagc gcgatataac ggacggtttt aacagggata aatgccagta cgcaggcgaa
     3961 gagagtcaga aacgcaaggg ttaatgtcct aggcaattgg ggcaaaatca gcaacggaaa
     4021 aattccgcaa attatgcata cgccgactgt cgttattttc atgcacccct ccctgtttat
     4081 ggaaagtgtg caactttgtt gagcggttgt ctggtgggaa ttttgcgttc cccgaggcac
     4141 attcagaaga atttaacgaa agggtgaaaa ctgctcaaaa ctctgcgagg cagcttccag
     4201 ttcctgatta gagaattgaa aaaagcaccc gacaggtgct tttctcgcgt tcaagtttga
     4261 gtaaaaactt aaccgtaaat attggcgcga tcgcgcagtt ctttaccagg tttaaagtga
     4321 ggaacgtatt ttccttccag ttctacttta tcgccagtct tcggattacg tccggtacgt
     4381 ggtgcgcggt agtgcaaaga gaaactgccg aaaccgcgga tttcaatacg ctcgccctgc
     4441 gcaagagtcg aggccatatg ctccagcatc tcttttactg catcttcaac cgtcttggcg
     4501 ggaatgtgcg attgctgggt ggcaagtctt tctatcaatt ctgacttggt catgattcct
     4561 ccggttcctt aaaggcaaat taagcggctg ctgttgctgc attgattaag ggcggccgta
     4621 gccgccctta gtgcttgatt acaggacgaa acctgcaatc tgtcaagtaa actcaacaaa
     4681 cttcggaata aaaatcccga agagtcagag aattactcgc ctttagctgc tttgaaagct
     4741 tcagccattg cgttgttgga gaagtttgca tcttcctgtt tgttaacagt tgcgattgca
     4801 tctttctcgt cagcttcgtc tttcgcacga acagacaggc tgattgcgcg gtttttacga
     4861 tcaacgccgg tgaatttagc ttcaacttcg tcgccaacgc tcagaaccag ggtagcgtct
     4921 tcaacgcggt cacgggatgc ttcagaagca cgcaggtaac cttcaacgcc gtcagccagt
     4981 tctacggttg cgcctttagc gtcaactgca gttactttac cggttacgat agcgcctttc
     5041 ttgttcagag caacccagtt gttgaacgga tcttctgcga gctgtttaac gcccagggag
     5101 atacgttcac gttctgcgtc aacctgcaga acaactgcag cgatttcgtc gccttttttg
     5161 tattcacgaa ctgcttcttc gcctgcaacg ttccaggaga tgtcagacag gtgaaccagg
     5221 ccgtcgatgc cgccgtccag gccgatgaag ataccgaagt cagtgataga cttgatttta
     5281 ccttcaacac ggtcgccctt gttgtgggtt tccgcgaact gctgccacgg gttagctttg
     5341 cactgtttca gacccaggga gatacgacga cgttcttcgt cgatatccag aaccataact
     5401 tccactacat cgccaacgtt aacaactttg gacgggtgga tgtttttgtt ggtccaatcc
     5461 atttcggaaa cgtgtaccag accttcaacg ccttcttcga tttcaacgaa gcagccgtag
     5521 tcagtcaggt tggtcacgcg accagtcagt ttggtacctt ccggataacg tttagcgata
     5581 gctacccacg gatcttcgcc cagctgtttc aggcccaggg atacacgggt acgttcgcgg
     5641 tcgaacttca gcactttaac agtgatttcg tcgcccacgt tgacgatttc gctcggatgc
     5701 ttaacgcgtt tccaggccat gtcagtgatg tgcagcaggc cgtcaacgcc gcccagatca
     5761 acgaatgcac cgtagtcagt gaggttctta acgatacctt taacttccat gccttcctgc
     5821 aggttttcca gcagctgatc gcgctctgcg ctgttttcgg attcgataac ggcacgacga
     5881 gaaacaacaa cgttgttgcg cttctgatcc agcttgatta ctttaaattc aagctctttg
     5941 ccttccaggt gcagagtgtc acgcaccgga cgaacgtcta ccagagaacc tggcaggaac
     6001 gcacgaatac cgttcagctc aacagtgaag ccgcccttaa ctttgccgtt gataacaccg
     6061 gtaacagttt cagcatcttc gtaagctttt tccagcgtga tccaggcttc gtgacgttta
     6121 gctttctcac gggacagcag agtttcaccg aagccgtctt ctactgcgtc cagagcaacg
     6181 tcaacttcgt cacctacctg gatttccagc tcgccctggg cgtttttgaa ctgctcagcc
     6241 gggatggcgg actcagattt cagaccagcg tcaaccagta ctacgtcttt gtcgatagca
     6301 acaacaacgc cacgaacgat agaacccggg cgggtttcga tttcttttaa ggactcttca
     6361 aagagttgag caaaagattc agtcatgttt aatcttcagg tttatattta acgtccacct
     6421 ggctccatgc cggatggggt tgtttaacat acccgctgtc attccattgc aacgggggta
     6481 ctgcaaattc ggtcgcttat gcgagagcca atttctggcg cgcgtattgt agcgcttttt
     6541 caatcaattg ctcaatgctt aaggtggtgg aatccaacac taaagcatcg gctgccggaa
     6601 ccagtggcgc taccgctcgg ttacgatcgc ggtcgtcgcg ttctttgatc tcggccaaaa
     6661 ggcgctcgaa gttaacacta aagcccttct cctgcaactg tagcatgcgg cgatgcgcac
     6721 gttcttccga ggaggcgtca aggaaaattt tcactggtgc atcagggaat accaccgttc
     6781 ccatgtcgcg gccatcggca atcagacctg gtaattcgcg aaacgcgcgt tggcgacgca
     6841 ataatgcttc acgaacgcgt gggaatgccg cgacttgtga agccgcattc gccacttcct
     6901 gagtacgaat ttcgccgctg acatcttccc cttcgaggat cacttccaga ttgccattgg
     6961 tcgacacaaa acgtacatcc agatgggatg ccagcggtac cagcgcatct tccgacgcaa
     7021 catcaacatg gtgatgtaat gccgccaatg ccagtacgcg ataaattgca cccgagtcca
     7081 gcagatgcca ttgcaacgct tccgccatag ccttacacaa ggtgcctttc cctgcaccgc
     7141 ttgggccatc aatggtaata accggggcaa ttgccgtcat ctttatctcc ttaaaacagg
     7201 cgtaccgtta acataaccgc gcgcattata cgcgccgacg ccacaaatcg ttaacgttgt
     7261 gtgcaaaaac cagttttgtc tgtattcaga tggggattta ttgcgcaatt atagcgggct
     7321 ggcagaagac cagcccgaaa gatgacaatg atttacttac cagaggcgat acgatcacgg
     7381 atgtgttgcg cacgctctgt tgatggtggg tgagagtcaa acatggactg ggtgcgaccg
     7441 ccatccaggc tagccagttt ttcaaagctg ccaaccagcc cctgggtgct gatgccacgt
     7501 ttcttcaaca gatcaaaaga gaaatcatct gcttcggact ctttatcacg ggagtattta
     7561 gcattaatag cgccttctgc gatatcaccc aattgtgagc gggaaagctg ggaagccaca
     7621 ccgctggtag ctgaaatggc atcgcgtgcg gcaacgatcg cataagaagc tttcatttca
     7681 gccagcgagt gacccaacgc gacgtggccc agttcatggc ccagaacacc ttcaatttcg
     7741 ttgtcattca tcatgtccat caggccactg tagacacgaa cacagccgtt cgccatcgcc
     7801 catgcgttga cgtcgctggt catataaacc ttatagttga ccggcgtgcc attaatattg
     7861 ttacccaatg ctttggcgat ttttgccaga cgtttaccgt acttgctgga tttacttgcg
     7921 acttgattgc cgctgtccat ttcggcacag ccctgattgg caatcgcttt tgcatcggca
     7981 tcgcttaatg ttgctgcttt ataggcattt aaaccggagc tgatagccat attggtatca
     8041 ataccgtggg tattttgaca ccctgtcagt aatgctgcag aggtcgcaat cgccagcagt
     8101 aatttagtat tcttcatgtg aatcatccgt tgattaatca tatataaata ttatgtatca
     8161 acaacttaca ttgacaagcg acgctaccat taatcaaaat gactcacaag gtccgaaaaa
     8221 aaacgccgac aatgtgc
//
LOCUS       DADLTY010000180         8171 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.181, whole genome shotgun sequence.
ACCESSION   DADLTY010000180 DADLTY010000000
VERSION     DADLTY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.181"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            1..955
                     /locus_tag="JN334_003511"
                     /pseudo
     CDS             1..955
                     /locus_tag="JN334_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1127..1234
                     /locus_tag="JN334_003512"
     CDS             1127..1234
                     /locus_tag="JN334_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439535.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1287..2045)
                     /locus_tag="JN334_003513"
     CDS             complement(1287..2045)
                     /locus_tag="JN334_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HAY7439536.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2332..3261
                     /gene="pfkB"
                     /locus_tag="JN334_003514"
     CDS             2332..3261
                     /gene="pfkB"
                     /locus_tag="JN334_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HAY7439537.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3362..3652
                     /gene="ghoS"
                     /locus_tag="JN334_003515"
     CDS             3362..3652
                     /gene="ghoS"
                     /locus_tag="JN334_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY7439538.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3758..4618
                     /locus_tag="JN334_003516"
     CDS             3758..4618
                     /locus_tag="JN334_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAY7439539.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4659..5195)
                     /locus_tag="JN334_003517"
     CDS             complement(4659..5195)
                     /locus_tag="JN334_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439540.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5342..6010
                     /gene="hxpB"
                     /locus_tag="JN334_003518"
     CDS             5342..6010
                     /gene="hxpB"
                     /locus_tag="JN334_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HAY7439541.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6173..6763
                     /locus_tag="JN334_003519"
     CDS             6173..6763
                     /locus_tag="JN334_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7439542.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6896..8171
                     /locus_tag="JN334_003520"
                     /pseudo
     CDS             6896..8171
                     /locus_tag="JN334_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
BASE COUNT         2124 a         1954 c         1954 g         2139 t
ORIGIN      
        1 gctcagacct gggctgatag gtatgttcgc aactgtgcaa ttgctattct gcccctatta
       61 cctgtgggag ttctaggggc tattttcgcg ggtattgcat cacgaatgcc atttgcccct
      121 atacatccat cggcaatgtt gttaataatg gctacaggcc agactcaaca gcttattaca
      181 ttattcaaac agttgcccat actccctgaa aaagaaatca ttgaaataat aactgcgcag
      241 aatagcgttg gtacacctgc tttatttttg gctatgatga acggacatac tgacaacgtg
      301 aaaatattta tgcaagaaat tcagtcactg gtagataatc acatcattca tgaagataat
      361 ctggttaaat tactgcaaac taaaagtgct aacgaaacac ctggacttta tatctccatg
      421 ttgtatggat tcgatgaaat aatcgatatc tttctgaata cattaaccac tccaatagca
      481 ctaagagctt ttaaacaaaa aactggtgat gagtatttta gccatgaaaa tacatgatgg
      541 tgagccagga ttatacgccg caatggaaaa taatcaccct ttgtgtgtca cacggttcct
      601 ctctaaaatt aatggcatcg cctttaaata caagttgagc aaagctaaca tcatggattt
      661 attaaaagga gctacagcac agggaacccc tgcattatac atcgccatga gcaagggtaa
      721 tgaagacgtc gtgttatctt atatatcgac gctgggtgct tttgcaaaaa aacattcttt
      781 tagtcaacat cagttattta cactattggc cgctaaaaat catgacaaca tgtcagctgt
      841 tcatatagcc attcatcata agcattataa aactgtagaa acatattatg ctgctataaa
      901 tgtcatcagc caaagcctga gttttagtgc tgatgaaata aagacgtatt tataaccagc
      961 tatttacgat atatatcgat acataatttt atttttttca taaaacgcag aaacagtgac
     1021 catgtgcaca acgccacccg acggctgtaa taacaaaaaa tcccttgatg cctgtccctt
     1081 ttgttacact ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg
     1141 ctttgataaa aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt
     1201 gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac
     1261 cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga
     1321 cggtcggtat gttccggtgc agactctgga ggttcagagt tccatgtgac gttgtaagcc
     1381 accttcagtc caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt
     1441 gtgtcttccg cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc
     1501 catgcataag ccccggaggc atagcccagc ggctgggttt cactggcatt gtcggtatat
     1561 ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa
     1621 aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct
     1681 gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa gcggctacgc
     1741 ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc
     1801 ccccacagcg accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt
     1861 gaggagcttt tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa
     1921 ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca
     1981 ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc
     2041 ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct
     2101 taaggatcaa agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact
     2161 tagcaatgta ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt
     2221 atatatattt atctgcaaaa ttttaaataa agctctaata aatcatattg ttaatttctt
     2281 cactttccgc tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt
     2341 atctatacgt tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat
     2401 cccgaaggaa aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac
     2461 gtcgcccgcg ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc
     2521 gcgaccggcg aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta
     2581 gaagccaaag actggacccg gcagaattta cacgtacatg tggaagcaag cggtgaacag
     2641 tatcgttttg ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag
     2701 caagttctgg aaattgaatc cggggccatc ctggtcataa gcggaagcct gccgccaggt
     2761 gtgaagctgg aaaaattaac ccaactgatt tccgctgcgc aaaaccaagg gatccgctgc
     2821 atcatcgaca gttctggcga agcgttaagt gcagcactgg cgattggtaa catcgagttg
     2881 gttaagccta accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccagac
     2941 gatgtccgca aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt
     3001 tcccttggtc cacaaggagc gctgggtgtc gatagtgaaa actgcattca ggtggtgcca
     3061 ccaccggtga aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca
     3121 ctgaaactgg cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg
     3181 agtgcagcca cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt
     3241 tacgcttacc tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac
     3301 ctgtcggcaa cgcgtttctc cgactatgct caaaagtcat gtgataacaa aggggtgaac
     3361 tatggccagt ggcgatcttg tccattatgt cataaccgtc atgttgcatg aggatacatt
     3421 gactgaaatt aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac
     3481 ggatgatgag ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca
     3541 aagtgaagaa gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga
     3601 tcctgaaatc accatcacga cctgggagga atggaatagc aacagaaaat aaatggtttt
     3661 tgggcaataa tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct
     3721 aaccttatga tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct
     3781 tttgagcgag cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga
     3841 agtccatgcc gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga
     3901 aagggaactg cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag
     3961 taaaaccgtc accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct
     4021 ggtgatggat tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca
     4081 gcaaattgcg cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa
     4141 ctcgctctcc acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc
     4201 tgaacaacgg attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa
     4261 tatcgacgcc atcgtcgagc atattcagca gcgtctggcc tcgcatcaac cgcagccttc
     4321 tctgttgcac ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat
     4381 tttcgaccct gcctgcgact ggggtgaccg agagtgcgac ctagcgatgt taccgctgca
     4441 tactgaacag ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga
     4501 tttccttgaa cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caagattatt
     4561 tggcggtcag catttggtta ttgctcagca gtcattggat agattattag cagcatgata
     4621 tgggttgagg ataatggccg ctccgtgcgg ccttttgatt aaataaaccc aagcagagaa
     4681 aagaacacat aacctgcagc aatacaaata accggcagga tatatagtga gaaaaactgc
     4741 aggaaaatag tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc
     4801 tcttcccctt tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc
     4861 gcctggcgac tcatccgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg
     4921 aaaatcaccc agaacataac gttcacacca ccattaaaat taggggtcgg ggagttatac
     4981 cagaacagat tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagcaaag
     5041 tcgagcatga ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc
     5101 aaagaaatca atgtagagat caatgcaggt ataaaaatca cccaacccaa aatccttttc
     5161 aaaacagcaa tgcgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc
     5221 gtttcactta gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata
     5281 ggcgcttaat cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt
     5341 aatgtcaacc ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga
     5401 ctcagaacct ttatgggatc gagccgaact ggatgtgatg gcaagcctgg gggtagatat
     5461 ctcccgtcgt aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct
     5521 ttggtacgcc cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat
     5581 tgcccgtgcc atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc
     5641 tgttgcgtta tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca
     5701 tatgctggaa aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc
     5761 ggctgaaaaa ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa
     5821 actgggcgtt gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc
     5881 ttctaaagca gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc
     5941 acgttttgta ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct
     6001 tctcggttaa tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca
     6061 ttcaactgac ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg
     6121 cactgtataa aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc
     6181 ggaaggtcac cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac
     6241 gcccgtgctg gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt
     6301 gttaccggac atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa
     6361 accgatcgcc cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc
     6421 gctgctggca accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct
     6481 acaaggaatg gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg
     6541 tgttcccctg ctctggccat gccgctggcg tttccgcttg cctatcctgg ttccccaaaa
     6601 gggcaaccaa ctggaacgtt ttatctgcat ggcattattt gtctggtcgg tatggatgcc
     6661 ccattcatta cccgagaaca gcgctgttcg ttggtcatcg caaatgatca ataccttgca
     6721 gatccagttt catcggctta ttaagcatca ggttgaatac taaaaaggca aaaatcacct
     6781 ttctggaata agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc
     6841 gccaacacta tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa
     6901 ctttccatta attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac
     6961 ccgccataaa cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt
     7021 ttttggcctt gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt
     7081 acagtggttt aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc
     7141 gttagtcttc gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg
     7201 caaaatcagt tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt
     7261 cggtgtgctg gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc
     7321 agaaactgca cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatcagag
     7381 cgttccgcag ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc
     7441 caatccgacg tcaattatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt
     7501 gaaactgctg aaggatgatg cgccgaaagg ggaacgcgtc ttaaccgcta tcgataccct
     7561 acaaagctgg gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct
     7621 ggctctaatg accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag
     7681 tttcgttgtc gcgtcctacc tcggtctgct gattatgttt gctgtgcatg gcattctgct
     7741 gggcattaat ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt
     7801 tgcctttacc agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg
     7861 tcgtctgggc gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg
     7921 tcagaacggc tgcgccggtt tgtatccggc aatgctgcgg tgatggttgc gcctacggtt
     7981 ggcattaacc cgctggaccc gatgtggatt gcgacgctgg tcggtattgt taccgttagt
     8041 tccgcaggcg ttgccggtgt cggtggtggt gcaactttcg ccgcactgat tgtgctgcct
     8101 gcgatgggcc tgccagtaac cctggtggcg ctgttaatct ccgttgaacc gcttatcgac
     8161 atgggccgta c
//
LOCUS       DADLTY010000181         8133 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.182, whole genome shotgun sequence.
ACCESSION   DADLTY010000181 DADLTY010000000
VERSION     DADLTY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.182"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..1100)
                     /locus_tag="JN334_003521"
     CDS             complement(<1..1100)
                     /locus_tag="JN334_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAY7439543.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="JN334_003522"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="JN334_003522"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HAY7439544.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="JN334_003523"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="JN334_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HAY7439545.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="JN334_003524"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="JN334_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HAY7439546.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="JN334_003525"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="JN334_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HAY7439547.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="JN334_003526"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="JN334_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HAY7439548.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..4381
                     /gene="pspF"
                     /locus_tag="JN334_003527"
     CDS             3404..4381
                     /gene="pspF"
                     /locus_tag="JN334_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HAY7439549.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="JN334_003528"
     CDS             complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="JN334_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HAY7439550.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5804..7084)
                     /locus_tag="JN334_003529"
     CDS             complement(5804..7084)
                     /locus_tag="JN334_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY7439551.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7086..>8133)
                     /locus_tag="JN334_003530"
     CDS             complement(7086..>8133)
                     /locus_tag="JN334_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY7439552.1"
                     /translation="HELAMIVREPVGVIAAIVPWNFPLLLTCWKLGPALAAGNSVILK
                     PSEKSPLSAIRLAGLAKEAGLPDGVLNVVTGFGHEAGQALSRHNDIDAIAFTGSTRTG
                     KQLLKDAGDSNMKRVWLEAGGKSANIVFADCPDLQKAASATAAGIFYNQGQVRIAGTR
                     LLLEESIADEFLALLKQQAQNWQPGHPLDPATTMGTLIDSAHADSVHTFIREGEGKGQ
                     LLLDGRNAGLAAAIGPTIFVDVDPNASLSREEIFGPVLVVTRFTSEDQALQLANDSQY
                     GLGAAVWTRDLSRAHRMSRRLKAGSVFVNNYNDGDMTVPFGGYKQSGNGRDKSLHALE
                     KFTELKTIWISLEA"
BASE COUNT         1871 a         2192 c         2078 g         1992 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaataatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtggaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggaagggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccatgtaa
     1081 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg agctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaaaaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataacttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
     4081 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
     4141 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
     4201 ttcagaaaat acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca
     4261 ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc
     4321 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
     4381 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
     4441 tcaatacgtc gtttgcaggc ctgataagcg cagcgcatca gacattattg cgttgggcta
     4501 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat
     4561 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
     4621 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
     4681 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc
     4741 cggaacgctt tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc
     4801 gcgttcgcag agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc
     4861 cagcgggttc ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt
     4921 cgcgttaccg accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat
     4981 taagtccggc ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc
     5041 gctttgcact tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc
     5101 ggcaaccagc tcttttggcg caacgttgaa accgcactcg ccctgcaccg gttcgaaaat
     5161 aatggccgcg acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag
     5221 ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc
     5281 agggaacggg ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat
     5341 atatgtacga ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc
     5401 gcgagcaatt ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt
     5461 agcctgcccg ctcactgggg caagggtgtt aattttctcc gccagcgtga cgtagctttc
     5521 gtacggcaca atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc
     5581 gaccagatca gggtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat
     5641 gtattcgttg ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt
     5701 acacatcacg ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt
     5761 gctcatgttt cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat
     5821 cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc
     5881 cggggaacgg gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca
     5941 gcagttcagc aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac
     6001 cctgcatgta atagatgttg gtatcaaggc ggccgaactg cggcatgtgc gacaaggtca
     6061 gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt
     6121 ttagtagttt cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta
     6181 cacccccgcc gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt
     6241 tacagtcttc cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc
     6301 gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta
     6361 ctttatcgcc cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat
     6421 tggcagtccg aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat
     6481 acacgcgccc gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt
     6541 gaatatgccc accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc
     6601 ggcgaatagc gttgacgtcc agcagctcca gctgtttatt gccgtagcgt acccagttct
     6661 ctttctgctc ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg
     6721 ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct
     6781 cgaacatcat gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt
     6841 cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc
     6901 cgatgcgtga ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg
     6961 aggagagccc ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca
     7021 gtgtgtcgaa tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg
     7081 tcatttcagg cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca
     7141 tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata
     7201 tcgccgtcgt tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg
     7261 tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg
     7321 gcaagctgca gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa
     7381 atctcttcgc ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg
     7441 gccgctgcca gcccggcgtt gcgaccatcc agcagcagtt gccccttgcc ttcgccttca
     7501 cgaataaagg tgtgtaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt
     7561 gcgggatcaa gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag
     7621 aattcatcgg cgatgctctc ttccagtaac aaacgcgttc cggcgatgcg cacctggccc
     7681 tggttgtaga agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg
     7741 gcgaaaacga tgttggcgct tttgccgccc gcttccagcc agacacgttt catgttgcta
     7801 tcacccgcgt ctttcagcag ttgtttaccc gtacgcgtgg agccggtaaa ggcgatggca
     7861 tcgatatcgt tatgacgcga aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg
     7921 ttcaacacac catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg
     7981 agcggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag cgccgggccg
     8041 agtttccagc aagtcagcaa cagcgggaag ttccacggca cgatggcggc aatcacgccg
     8101 accggttcac gcacgatcat cgccagctca tgg
//
LOCUS       DADLTY010000182         8119 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.183, whole genome shotgun sequence.
ACCESSION   DADLTY010000182 DADLTY010000000
VERSION     DADLTY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8119
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.183"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            1..1459
                     /locus_tag="JN334_003531"
                     /pseudo
     CDS             1..1459
                     /locus_tag="JN334_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(1428..3165)
                     /gene="aceK"
                     /locus_tag="JN334_003532"
                     /pseudo
     CDS             complement(1428..3165)
                     /gene="aceK"
                     /locus_tag="JN334_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(3349..4653)
                     /gene="aceA"
                     /locus_tag="JN334_003533"
     CDS             complement(3349..4653)
                     /gene="aceA"
                     /locus_tag="JN334_003533"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HAY7439553.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4683..6284)
                     /gene="aceB"
                     /locus_tag="JN334_003534"
                     /pseudo
     CDS             complement(4683..6284)
                     /gene="aceB"
                     /locus_tag="JN334_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(6553..7482)
                     /gene="metA"
                     /locus_tag="JN334_003535"
     CDS             complement(6553..7482)
                     /gene="metA"
                     /locus_tag="JN334_003535"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAY7439554.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7639..8082
                     /locus_tag="JN334_003536"
     CDS             7639..8082
                     /locus_tag="JN334_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAY7439555.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2050 a         1999 c         2025 g         2045 t
ORIGIN      
        1 accatgcttt gacggtccgg ctaagaataa agaacacaac tgaaggatgt acccattacg
       61 tggtttcggt ttatgatcct aatgtaacta acgataaaat aagaattatg agcgaaagca
      121 aagaggatat taaacactat tctctgatgg attttatgaa tgtagattat agcctcctga
      181 aatggtcaaa tgatcatgtt attaaccaat ctgttgcaat aattccagca cttccgaaag
      241 aacagctatt gatgttaaaa ggaactgtgg atgaaataac ccctccatta tcaccagcaa
      301 cgatgaattt gctaatggca attggtcaga atcaccaact tacgcaactg atgattcagc
      361 tccagaaaat gccagaacta catagaacag aaatgttgac tgcctataat agtattaacc
      421 tccccggttt atatttggct ataaattatg gcaatgcgga tatcgttgag actattttca
      481 attcattgtc agaaacagga tatgaagggt tactctcgaa aaaaaatctc atgcatattc
      541 tggaggcaaa agataaaaat ggtttttctg gattattttt agcgatatca cgtaaggata
      601 aaaatgttgt aacctcgatt ctgaacgtct tacctaaact ggccgcaaca catcatttag
      661 ataatgaaca ggtgtataaa ttcctgagtg ccaaaaatag aacgtccagc catgttttat
      721 accatgttat ggcgaatggt gatgccgaca tgctgaaaat ttttttggtc gcgttacctt
      781 tgttaattcg cacatgtcat ttgactaaag aacaggtact cgatctcctg aaggcaaagg
      841 atttttatgg ttgcccaaga ctatacctgg cgatgcaaaa tggacatagc gatatcgtga
      901 aagttattct cgaagcattg ccctgtctgg cccaggaaat taacatttca gcttccgata
      961 ttgtagatct tctgaccgct aaaagtcttg cgcgcgacac gggtttgttt atggccatgc
     1021 agcgcggaca tatgaacgtt attaatacta tttttaacgc attacccact ctttttaata
     1081 cgtttaaatt cgataaaaaa atatgaagcc cctcctcctg gcaaataatt ctaatgaata
     1141 tcccggtttg ttttcagcga tacagcataa acaacaaaac gttgtagaga cggtttatct
     1201 tgctttatct gaccatgcac gcctgtttgg atttaccgct gaagatatta tggatttttg
     1261 gcaacacaaa gcgccacaaa aatactctgc ctttgagttg gcttttgaat ttggtcaccg
     1321 ggttattgct gaattaatcc ttaatacatt aaataagatg gctgaaagct ttggctttac
     1381 ggataaccct cgatacattg cggagaaaaa ttatatggaa gctttactca aaaaagcatc
     1441 tccccatacc gtacgctaaa tctttgcctg cgccgatacg cataaacatc ttccacatgc
     1501 ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag gtcggcgtgc
     1561 atctcttcaa acagcggacc aatacgcggg tcggcgcata gccagtggcg aaactcttcc
     1621 gggaaaacat cgcccggcga gacgctgtac cacggttcgc tggcaagttc gtcttccgga
     1681 tagcgcggcg gcgggatgtc gcggaaattc acttccgtca tgtagcaaat ttcatcgtaa
     1741 tcataaaaaa ccacacgccc gtgacgggtg acaccgaagt ttttaaacag catgtcgcca
     1801 gggaaaatgt tagcagcggc aagctggcga atagcgttac cgtattcttc aatggcgtcg
     1861 cgcaactgct gaccttccac ttgttccagc cagatattga gcggcaccat ccgccgctcg
     1921 attatagaga tggcgaatta caatctgctc accaagatcg gtgatttttt ccgctgcttc
     1981 ctggagcagt aattccatta ataccgggga aatatgccgc ttctccagca caaagttttc
     2041 aaactcctgg gtgtccgcca ttcggcccac acggtcgtgc tctttcacca gttgatagca
     2101 ggcacgaacg tgagcggcag acatctcttt ctgcggcgcg aacttgtctt tgatgacttt
     2161 gaatacccga tcaaagcccg gcagcgtaaa caccaacatc accattccac gaatacccgg
     2221 cgcttcaatg aactgctcat tacagccctg tagataaacg agatattcgc ggtagctttc
     2281 ggttttggcg tgcttctggc agccgatagc catatacaat tcagcagtgg ttttacctgg
     2341 cagaatttcc cgtagccact cgaccagcgc tgcgggcagc ggtgcataaa ccataaaata
     2401 agaacgcgca aagccaaaaa caatgctcgc ttcggcggtc gtcgtcaggc aggtatcaat
     2461 aaataactcg ccgtcgtccg tctggtggat cggcagtaag aagggcaacg tgccggaagg
     2521 cgtgatcagt ttgcccacca gccaggcggc tttattgcgg taaaacagtt cgttcgccac
     2581 ctgtaaatga ctttccgcga ggttgtctgt ccccagcgtt tccgtcagat ggcgaatgat
     2641 gtaatgaatg tcacggcttt tattctgcca gcgcaggcgc agcggcaggt cgctgataac
     2701 gcgcatcagt agagattccc agccgtgatc ggggtgaaag tctttcgcca gcgggcgggg
     2761 aatggtacga aagcggcgct ctggctgaga gctaaagata aaaagccgct cgggagtaag
     2821 cgagcggtgg tcaaataacc gacagtacac ggagttaaaa aagctctccg caatctcgaa
     2881 gcgcgggtaa tccggcaaca gccgcgtgta atgctcttta acgcgcagta aaaatgccgc
     2941 gtccgtactt tggccgttag taatgcagcg cagttgctcc acgaccagac caacgtgatg
     3001 atcgtaaaga tggatacggt ttttcatcgc ctgctggaca gcatgccagt cggcctgttc
     3061 gaaacgctgc tgcgccccgg aagtcacttc gaggaatcga ccatactgag cgtcgaagcc
     3121 ttgcaaaatg gtttgagcaa tcagtaattc caggccacgc ggcatttagc gccctcatca
     3181 ggagcagaga attgccggat gcggcgtaaa cgccttatcc agcctacgtt cggcaacggt
     3241 tgtaggcatg ataagacgcg caagcgtcgc atcaggcatc gtgcaccgat tgccggatgc
     3301 ggcgtaaatg ccttatccgg cctacagtca gcaacgtttg taggttgctt agaactgcga
     3361 ttcttcagtg gagccagtca gcgcggtgac tgaagacgtg ccgccctgaa taatagtcgt
     3421 cactttatcg aagtaacctg tacccacttc ctgctggtga gatacgaagg tatagccatc
     3481 tttcgcggcg gcaaattccg gctgctgcac tttctcaaca tagtgcttca taccttcgcc
     3541 ctgcgcataa gcgtttgcca ggtcgaacat gttaaaccac atgctgtgga tacctgctag
     3601 ggtgatgaac tggaacttgt agcccatatc cgacagctgc tgctggaagc tggcaatagt
     3661 tttgtcgtcg aggtttttct gccagttgaa cgacggcgag cagttataag ccagcagttt
     3721 gcccggatat ttcgcgtgga tagcttgtgc aaagcgacgc gccagttcca gatccggcgt
     3781 ggaggtttca caccagacca ggtcagcata tggcgcatac gccaggccac ggctgatcgc
     3841 ttgctcaatg cccgcatgag tacggaagaa gccttcactg gtacgctcgc cggtaataaa
     3901 ttcgctgtca tacgggtcgc aatctgaggt gatcagatcc gccgcatcag catcggtacg
     3961 tgcaaccagc agggttggaa cgcccgtcac gtcagctgcc agacgcgccg cgaccagttt
     4021 ctgaatagct tcctgagttg gcactaaaac tttgccgccc atgtgaccgc atttcttcac
     4081 tgacgccagc tgatcttcga agtgaactgc cgcagcaccg gcttcaatca tcgctttcat
     4141 cagttcaaag gcattcagga caccgccaaa accggcttcc gcatcggcaa cgatcggcag
     4201 gaagtaatcg acatagcgcg gatcgcccgg ctcaatgccc acggaccatt ggatctgatc
     4261 ggcacgacgg aaggtgttgt tgatccgctc caccacagct ggcaccgagt ttgccggata
     4321 gagcgactga tccggataca tgctggccgc caggttagcg tccgccgcta cctgccatcc
     4381 cgacagatag actgcttcaa tacccgcttt cgcctgttgc agcgcctgac cgccagtcag
     4441 tgcaccgagg ttgttgatgt agcctttttt cgactcaccg tgcagcagac gccacatttt
     4501 cgctgcgccc agttgcgcca gcgtgcattc aggattgact gaaccgcgta atttcaccac
     4561 atcttccgca ctgtatgggc gagtaatgcc ttcccaacgc ggttgagtcc actctttctg
     4621 taattcttca atttgttgtg tacgggtttt catgtgcaga tgctccatag ttatgtggtg
     4681 gtttacgcta acaggcggta gcctggcagg gtcaggaaat caattaactc atcggaagtg
     4741 gtgatctgtt ccatcaagcg tgcggcatcg tcaaaacggc cctgggagaa acgttcttcg
     4801 cccagttcgc tggcaatgac tttcatctct tcgcccagca tctggcggaa caaggctttg
     4861 gtcaccggtt tgccattgct caacgttttt tgatgatgga tccactgcca gatcgaggta
     4921 cgggaaattt cagccgtcgc cgcatcttcc atcaggccat aaatcggcac gcagccgttg
     4981 ccggagatcc acgcttcgat gtactgcaca gccacgcgaa tgttagcgcg cataccttct
     5041 tcggtgcgtt caccatcaca aggtgccagc agctgatcgg cagtaatcgg cgcgtcttgt
     5101 tcgcgcaata cttcaagctg atttttacgg gagccgagaa tgtcgttgaa taccgccatt
     5161 gccgtgtccg caaggcctgg gtgagcgatc catgtgccat cgtgaccgtt attggcttcc
     5221 agcgctttat ccgcttttac tttgttgagc acctggttat tacgctcttc atctttgctc
     5281 ggaataaacg cggccatgcc gcccatcgca aaagcaccgc gtttatggca ggttttaatc
     5341 agcaggcgtg agtaagcatt caggaatggt ttatccatcg tcacggcctg tctgtctggc
     5401 aggacacgat cgggatagtt tttcaacgtt ttgatatagc tgaagatgta atcccagcga
     5461 ccgcagttca gaccaacaat atggtcgcgc agcgcgtgaa ggatttcgtc catctggaac
     5521 acggcgggca gcgtttcaat cagcaacgtc gccttgatgg tgccgcgcgg cagattaaag
     5581 cgatcttctg catagctgaa gacttcgctc caccaggccg cttcctgcta ggactgggtt
     5641 ttcggcagat agaaataggg accgctgccc tttgccaaca gtgcctgata gttgtggaag
     5701 aaatagagcg caaaatcaaa caggctaccg gggattgcct caccacgcca ggtgacatgt
     5761 ttttccggca agtgcagacc gcgtacccga caaatcaaaa ccgctggatt gggcttgagc
     5821 tgataaattt tgcctgcttc attggtatag ctgatggtac cgttaaccgc atcgcgcagg
     5881 ttaatttgcc cgtcgatcac tttgttccag tctggtgcca gtgaatcttc gaaatcggcc
     5941 ataaagactt tcacattggc gttcagtgcg ttgatcacca tcttgcgctc taccgggcca
     6001 gttatctcta cgcggcggtc ttctaagtcc gcaggaatcc cgcgaatttt ccaatcagca
     6061 tcgcgaatgg aagccgtttc cgaaataaaa tcaggcaacg ttccgttatc aatatcttgc
     6121 tgctgctgaa tgcgcgctgc cagaagttta ttgcgttgtg gcgtaaaatg cgtcaccagc
     6181 tcagtcagaa attctaccgc ttcggcagta agaatttgct tctcctgctc gccatacggc
     6241 cttgtgaaag ccagttcatc ggttgttgtt gcctgttcag tcatcgtgca gctcctcgtc
     6301 atggatccga aaacttcccc tctgtgaacg aaggaacgtt gtgcgctttt cgttcagcac
     6361 aactaagact actcatttaa aatgcaaaat caaaaacaat ttccatttta attaaaaata
     6421 cttgataact tattgataac aatatgaata aaatttattc acttgctgag gtgcgtttcg
     6481 gaaataaaaa aggcacccga aggtgcctga ggtaaggtgc tgaatcgctt aacgatcgac
     6541 tatcacagaa gattaatcca gcgttggatt catgtgccgt agatcgtatg gcgtgatctg
     6601 atagacgtaa tagttgagcc agttggtaaa cagcaaatta ccgtgactac gccagctcgc
     6661 tcgcggtttg ttttgcggat cgttgtgcgg gaaatagtta tacggtacat ccgggtctag
     6721 tccggcttcc acatcgcgta aatattcctg cgccagcgtt tgcgcatcat attcgggatg
     6781 gcccgtcaca aaggcaatgc gcttatcttt gctggcaaac agatatgcat ccccttcttc
     6841 cgtctctgcc agaatttcca gatcggtgta atcacgaatc aacgctgccg gaaagtcagc
     6901 atagcgcgaa tgcggtgcca ggaatgaatc atcaaagcca cgcgtcagaa gcgcatgagg
     6961 atggagaata tgatgctcgt aaacgccaga gagtttgtcg gtgcgagttt gcttaggaat
     7021 gccgtagagg atattgagcg cggcctgtac cgcccagcag acaaacagcg tcgaggtgac
     7081 gtgatctttc gaccactcca gcacctgttt gatctgcggc cagtaagcga catcattaaa
     7141 ctccaccagg cccagcggcg caccggttac aatcagaccg tcaaagttct gttcctgaat
     7201 atcttcaaag ttacagtaga agttgttcag atgctctgcg ggcgtgttgc gcgattcacg
     7261 ggaatcgatg cgcaacagct gaatatcgac ctgcaaaggt gagtttgaaa gcaggcgcag
     7321 aaactgattt tcagtttcaa tcttcttcgg catcaggtta aggatcagaa ccttaagtgg
     7381 acgaatttcc tgaccagacg cacgagaagt tgtcatcaca aagacgtttt cttcacgcaa
     7441 gaaattgacg gcgggtagct cgtccggcac acgaatcggc ataacctgat tacctcacta
     7501 catacgctta tacgtttaga catccagata gctgaagata accagaaaat ttgccaatgt
     7561 cgagcctgca tgttgaaggt gagaaagttt caaggaaaag gctacgttag aatataagaa
     7621 tgacgaaaag gagagaaaat ggttattagt attcgccgct cacggcatga ggaaggggag
     7681 gaactcgttg cgatttggtg tcgatctgtc gatgccaccc acgatttttt atcagcagag
     7741 tatcgggccg agctggaaga gctggtgcgt tcctttctgc cggaagcacc gttgtgggtc
     7801 gcggttaatg aacgggagca gccggttggg tttatgttgt taagtgggca gcatatggat
     7861 gcgctgttta tcgatcctga tgtgtgcggc tgcggcgtgg gtcggatgct ggtggagcat
     7921 gcgctctcga tggcaccgga actgacaacc aacgttaatg agcaaaatga gcaggcggtt
     7981 gggttctata agaaggtggg ttttaaggtt acgggacgct ctgaggtgga cgatttgggg
     8041 aaaccgtatc cgttgctgaa tctggcgtat gtgggggaat aagccgcctc tgccgtgcct
     8101 gcccaagctg gagaagacg
//
LOCUS       DADLTY010000183         8031 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.184, whole genome shotgun sequence.
ACCESSION   DADLTY010000183 DADLTY010000000
VERSION     DADLTY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.184"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..104)
                     /locus_tag="JN334_003537"
     CDS             complement(<1..104)
                     /locus_tag="JN334_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HAY7439556.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="JN334_003538"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="JN334_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HAY7439557.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            1354..2004
                     /gene="csgD"
                     /locus_tag="JN334_003539"
     CDS             1354..2004
                     /gene="csgD"
                     /locus_tag="JN334_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HAY7439558.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2009..2398
                     /gene="csgE"
                     /locus_tag="JN334_003540"
     CDS             2009..2398
                     /gene="csgE"
                     /locus_tag="JN334_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HAY7439559.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2423..2839
                     /gene="csgF"
                     /locus_tag="JN334_003541"
     CDS             2423..2839
                     /gene="csgF"
                     /locus_tag="JN334_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HAY7439560.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2866..3572
                     /locus_tag="JN334_003542"
                     /pseudo
     CDS             2866..3572
                     /locus_tag="JN334_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3705..4196)
                     /locus_tag="JN334_003543"
     CDS             complement(3705..4196)
                     /locus_tag="JN334_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAY7439561.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4298..4852)
                     /locus_tag="JN334_003544"
     CDS             complement(4298..4852)
                     /locus_tag="JN334_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7439562.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4876..5613)
                     /locus_tag="JN334_003545"
     CDS             complement(4876..5613)
                     /locus_tag="JN334_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAY7439563.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5668..6606)
                     /gene="ghrA"
                     /locus_tag="JN334_003546"
                     /pseudo
     CDS             complement(5668..6606)
                     /gene="ghrA"
                     /locus_tag="JN334_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6841..6928
                     /locus_tag="JN334_003547"
     tRNA            6841..6928
                     /locus_tag="JN334_003547"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6875..6877,aa:Ser,seq:gga)
     gene            complement(7078..7359)
                     /locus_tag="JN334_003548"
                     /pseudo
     CDS             complement(7078..7359)
                     /locus_tag="JN334_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7641..>8031)
                     /locus_tag="JN334_003549"
     CDS             complement(7641..>8031)
                     /locus_tag="JN334_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY7439564.1"
                     /translation="DDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLV
                     PRQYSTGGKSTLLGISKRGDKNLRRLLVQCARSFIMRLERKHGKLAEWVREQLNKKHS
                     NVVTCALANKLARIAWAITTQKKEYQA"
BASE COUNT         2182 a         1929 c         1745 g         2175 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaactgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcctg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcgtc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaacaaaaac gttttacatg acgaaaggac tacaccgaaa tattttttat
     1021 atgtattatt agtaagttag caccatttgt atgatttttt aaaattgtgc aataaaaacc
     1081 aaatgtacaa cttttctatc atttctaaac ttaataaaac cttaatgtta acattttaat
     1141 ataatgagtt acattttgtt acatgtttaa cacttgattt aagatttgta atggctagat
     1201 tgaaatcaga tgtaatccat tagttttata ttttacccat ttagggctga tttattacta
     1261 cacacagcag tgcaacatct gtcagtactt ctggtgcttc tattttagag gcagctgtca
     1321 ggtgtgcgat caataaaaaa gtggggtttc atcatgttta atgaagtcca tagtattcat
     1381 ggtcatacat tattgttgat cactaaacct tctttgcagg cgacagctct cttgcagcac
     1441 cttaaacaat cgctagcaat tacaggaaaa ttacataata ttcaacgttc tctggacgat
     1501 atctcttcag gctctattat tcttctggat atgatggaag cggataaaaa gcttatccat
     1561 tattggcagg atactttgag caggaaaaac aacaatatca aaatattgtt gctaaatacg
     1621 cctgaagatt acccgtaccg cgacattgaa aactggcctc atatcaacgg cgttttttat
     1681 gccatggagg atcaagaacg tgttgtcaat gggttgcaag gcgtcctgcg tggcgaatgc
     1741 tactttacgc aaaagcttgc cagttacctg attacacatt ctggtaacta tcgttataac
     1801 agcacggaat cagccctcct tactcatcgg gaaaaagaga tcctgaataa gctgcgtatc
     1861 ggcgcgtcta ataacgagat cgctcgttcg ttgttcatca gcgaaaatac ggttaaaacg
     1921 catctttata atcttttcaa gaagatagcc gtaaaaaacc ggacacaagc ggtttcctgg
     1981 gcaaacgata acctcaggcg ataaagccat gaaacgttat ttacgctgga ttgtggcggc
     2041 agaatttctg ttcgccgcag ggaatcttca cgccgttgag gtagaagtcc cgggattgtt
     2101 aactgaccat actgtttcat ctattggcca tgatttttac cgagccttta gtgataaatg
     2161 ggaaagtgac tatacgggta acttaacgat taatgaaagg cccagtgcac gatggggaag
     2221 ctggatcaca ataacggtca atcaggatgt tattttccag acttttttat tcccgttgaa
     2281 aagagactcc gagaaaactg tcgtctttgc actgattcaa actgaagaag cactaaatcg
     2341 tcgccagata aatcaggcgt tattaagtac ggacgatttg gcgcatgatg aattctaaat
     2401 aaaaaattgt tcggaggctg caatgcgtgt caaacatgca gtagttctac tcatgcttat
     2461 ttcgccatta agttgggctg gaaccatgac tttccagttc cgtaatccaa actttggtgg
     2521 taacccaaat aatggcgctt ttttattaaa tagcgctcag gcccaaaact cttataaaga
     2581 tccgagctat aacgatgact ttggtattga aacaccctca gcgttagata actttactca
     2641 ggccatccag tcacaaattt taggtgggct actgtcgaat attaataccg gtaaaccggg
     2701 ccgcatggtg accaacgatt atattgttga tattgctaac cgcgatggtc aattgcagtt
     2761 gaacgtgaca gatcgtaaaa ccggacaaac ctcgaccatc caggtttcgg gtttacaaaa
     2821 taactcaacc gatttttaag ccccagcttc ataaggaaaa taatcatgca gcgcttattt
     2881 cttttggttg ccgtcatgtt actgagcgga tgcttaaccg ccccgcctaa agaagccgcc
     2941 agaccgacat taatgcctcg tgctcagagc tacaaagatt tgacccatct gccagcgccg
     3001 acgggtaaga tctttgtttc ggtatacaac attcaggacg aaaccgggca atttaaaccc
     3061 tacccggcaa gtaacttctc cactgctgtt ccgcaaagcg ccagggctta caaaacctgc
     3121 ttaacgagcg caaggttatt cgtgcggcac aagaaaacgg cacggttgcc attaataacc
     3181 gaatcccgct gcaatcttta acggcggcaa atattatggt tgaaggttcg attatcggtt
     3241 atgaaagcaa cgttaaatcg ggcggtgttg gggcaagata ttttgggatc ggtgcagaca
     3301 cgcaatacca gctcgatcag attgccgtga acctgcgtgt cgtcaatgtg agtaccggtg
     3361 agatcctctc ctcggtgaac accagtaaga cgatactttc ctatgaagtt caggccgggg
     3421 ttttccgctt tattgactac cagcgcttgc tggaagggga agtgggttac acctcaaacg
     3481 aacccgttat gctgtgtctg atgtcggcta tcgaaacagg ggtcattttc ctgattaatg
     3541 atggtatcga ccgtggtctg tgggatttgt aaaataaagc agaacggcag aatgacattc
     3601 tggtgaaata ccgccatatg tcggttccac cggaatcctg acagataaaa atgccggaat
     3661 taataatccg gcatttactg tttaagcgaa agcgtgagtg cccctcacgc tttatttttg
     3721 atttcctgct cacggtgagc cgtctttgca ctacgcgccg ccagccacag gccgctgttt
     3781 ttcattgcgt aaccaaatac cagcccaagc gccagcgaag gtaacaccag tttccagtca
     3841 ccctgcccgg caaacgtcgc gcatgcgcct ataaacgtcc ccggaacaaa tgaaagcagc
     3901 agctgtttgg cctgaataca catcagaaac gcgacaatac cggttatgac ataaccgaga
     3961 atttccagat gtggtgccag tgcactaccg taaataatga ccatcgccca caccacgcca
     4021 ctaagcaggg ttgcagcgga gatcgccagc cctttcagcc caccttgcgg gcaagcaaag
     4081 taggccgtac agcccaggaa gcccgcccag cttagtaagc ccagggaaac agccacccat
     4141 ccccagatac cggagagaat gcctgttgtg attgcaatag agagaagtat gttcatgccg
     4201 cgcatgatag cagaaaacgc ggccatgaat gagatcgagc acacatttta tgcaacaaca
     4261 aattatctgt tgcagttatg agtgacgatg atcacattta ctcttcagaa tcttcttcca
     4321 gctcgtccca cattgcacta atggcatcac gggttagcgg tgccatggtg cgccagaaag
     4381 gcgtggttgc atgggcctcc actttgccga ggaacgcacc acaccagggt aacagatact
     4441 cactgaacag tgtttccagt gcttcgctct catcttccgt tgactgatct tccagccagg
     4501 aagccgcaag cagcaatgtg ccgatgtgat cggctggcgt atccgccagt agcatccctc
     4561 gttcggaaag aaaagcgcgc acttccgctt ccgttgcgtc ctcaacccat gcgctacgat
     4621 atggcggcac ggcacattca tcgccgataa acaacgcatt gtaatcggca gagacttgcg
     4681 tcatatcaca gcttttctgt aaacgcgtca gtaactcatc ctgctccagt ggccagttcg
     4741 cagccagttt cccctcacga atcagagtaa acagcggcac cagtaaagga tcttgcggtt
     4801 ggcggtaata gagcgaaccc agcacacgac agaggataga aaactcgttc attaatatat
     4861 ttccattact agtaattaaa ggtctgcaaa ttccgcaatc ggtgccatac cgcgagattc
     4921 aaggaagttc agtaagcggc gcggagaaac attcaaaatg cgctctagcg gaaaatctac
     4981 cgcgtcgagg attttaagac actcttcaaa ttcccccatg gtaaacgcgg tgtgagaatc
     5041 cgagcctaat gccacccaac ctccagcatc acgtaccgct gcagctaccg cacgacagtt
     5101 gtcttcactg cccttacgtg agtgtaaaaa tgaggaatta ttgatttcca gcgccacctg
     5161 atgtttcgcg gctgcttcag caacggcttt aacatctatt tcatatttcg gatttccggg
     5221 atggcttatt atatgcacat tgccgctggc gatagtggag atcatcgctt gtgtattggt
     5281 cgctttgtca tgtggcgcaa aaaccggctc atgaaaaccg gcaataatca gatccagcga
     5341 gtcaaacatt ttaccgctgc agtcaatttc accatcaaca tttttaatgt tagcttcaat
     5401 gccgcgcagg atccctaccc catcaaccac tcgcggccag atacgcatgt taatgaagtg
     5461 ccagtgatgc ggcgcatctt ccatatccgg gccatgatcg gtgatcgcaa aaagtttaat
     5521 gcccttttgt ttggcctggg caatgtaatc actcaatgtg ctatatgcat gtgtgctggc
     5581 aacggtatgc atatgaaggt cgacgggata cataactctc tcctgtattc aattcccgcc
     5641 aaggatagca ggaatcctga tgctttatta gtagccacgt gcgcggtcga cttgcccgca
     5701 gaccctctcc cctttttcga gctgggcaat agtgcgagaa atgtactcca cagcttcagc
     5761 gggacgggta atcgcggcga catgtggtgt tatcgtcacg cgtggatgtt gccagagcgg
     5821 actttcaggc ggtaagggtt cacgattaaa aacatccagc attgcgcctt taactttccc
     5881 gctatccagc gccgcgagca ggtcatcttc cacaacatga acaccacgcg ccaggttgag
     5941 gagatacgcg ccatcaggta atttttcgag taattgttga ttaataatgc cgacggtttc
     6001 aggggtattc ggtaacaaat taatcaatac ccgacattgg ctcagaaatg cagacagttc
     6061 ttcccgtccg gcaaagcttt gcacgccagg ccacgattta cgggttcgac tccagcaacg
     6121 cagcggaaag cgctaggttt gcagactctg agcaacttta cagcccagta cgcctgcgcc
     6181 caaaatgccg atggtaaaat cttcccgatg atattcaggc agcggttgcc aatgcgaact
     6241 attttgctgg atgcgataat cgtcaaaacg tcgaaaccaa tgcagcacct gactgacagc
     6301 atattcctgc atttgctcgc ccataccggt atcttccagg cgaaaaagtg gaacagaagg
     6361 gttcagcatt tcagggtgtg cctgtagctt gctcaaaata gaatcaacac cggccccgag
     6421 tgcgaacacc gctttaagat cgcgccctgc cagcatttca acaggaggat gccagactaa
     6481 aacataatca gcagagtcat tatctccgct tttccatgcc ctgactcttg cctgaggaat
     6541 agctttgcgc agtgcctcaa tccaccattg ggtatcgaac gttgggtgat aaaagatgat
     6601 atccatactg actcccgaaa agcgttgtgc gaaatttatt cgcacttatc gttatgatct
     6661 acaaaggcca ccagcataac aaatccgtgg tcggtggcaa aaaaagcaga tttcgcttat
     6721 taaaaccaca cattgattga atgtttgaat aaacgcgcga ttttttcaaa aagtttgttg
     6781 acctcaggtc atgatttccc taaattagcg cccgttccag caagacagga acgacaattt
     6841 ggtgaggtgt ccgagtggct gaaggagcac gcctggaaag tgtgtatacg gcaacgtatc
     6901 gggggttcga atccccccct caccgccaga ttataagtgc atgttattgt ttaacatgcg
     6961 cttttttatt tttccttcca gacagtatgc catcgggtat gccatctgaa accggtttat
     7021 atgccccgcg atcatatctt atcggacaat ttccgatctt tgagcatact ggcaaagcta
     7081 tactgccagc atatcaggat agtgttttgc gataatatca ttcagtctgt caaccagttc
     7141 tggttttctg aacgtgatat gcgcggagcc cttcttaaag tatctgatgc tgaacatctc
     7201 atcttcatag ctctctttac cctgaacaga ctgaatgtga tcatccagac gaagggtgat
     7261 gtttccccgg ttgtcgggga ttggtttacc gctgaacagg tgcaacattc tttccagatc
     7321 ggcgagtcgg tcagcctgtt gtccggtgat aagatgaaat ctatggtcac ccccgttttt
     7381 gcaacactga tttttgatta atggttggct tgcttaaatc tatccggcgt ctctgtgggc
     7441 aaggatgccc gcgccccgat gagttccgca cctgataatc ctaaaaatcc cctcggcttc
     7501 agtgagccat ttcatctgtt aggtttttaa tcaggtcgat ttgccgttca tgtcatcaat
     7561 aatcagtctt cgcaaaacca gaaaggtaac ttgtatctta ttgtttaaaa taaactaatt
     7621 gagttatatc agcatctata tcaggcctga tattcttttt tctgagtagt gattgcccag
     7681 gcaattcgag ccagtttatt tgccagagcg caggtaacca cattcgaatg cttcttgttg
     7741 agttgctccc gaacccactc agcaagtttt ccatgcttgc gttcaagtcg cattatgaat
     7801 gagcgggcac actgaaccag taaccggcgt aaatttttat cacctcgctt actgataccc
     7861 aacagagtcg attttcctcc agtgctatac tgccggggaa caagtcctgt ggaagctgca
     7921 aaatccctgc tgcatgagaa ctgtttcccg tcaccaagtt gcgacgatag taagctggca
     7981 gtaatcggac caacaccagg aattgtcata atgcgctgag cggtatcatc a
//
LOCUS       DADLTY010000184         7879 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.185, whole genome shotgun sequence.
ACCESSION   DADLTY010000184 DADLTY010000000
VERSION     DADLTY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.185"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            101..428
                     /locus_tag="JN334_003550"
                     /pseudo
     CDS             101..428
                     /locus_tag="JN334_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(458..3370)
                     /locus_tag="JN334_003551"
     CDS             complement(458..3370)
                     /locus_tag="JN334_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439565.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3373..3489
                     /locus_tag="JN334_003552"
     CDS             3373..3489
                     /locus_tag="JN334_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7439566.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3853..4695
                     /locus_tag="JN334_003553"
     CDS             3853..4695
                     /locus_tag="JN334_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY7439567.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4698..5786
                     /gene="capU"
                     /locus_tag="JN334_003554"
     CDS             4698..5786
                     /gene="capU"
                     /locus_tag="JN334_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY7439568.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5791..6741
                     /gene="virK"
                     /locus_tag="JN334_003555"
     CDS             5791..6741
                     /gene="virK"
                     /locus_tag="JN334_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY7439569.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6806..7750
                     /gene="lpxM"
                     /locus_tag="JN334_003556"
                     /gene_synonym="msbB"
     CDS             6806..7750
                     /gene="lpxM"
                     /locus_tag="JN334_003556"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7439570.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2124 a         1763 c         1866 g         2126 t
ORIGIN      
        1 cgtaaagaag ggcatcaagc tcctgctccg acatacctgc aggcaaaggc catgaaagcc
       61 ctgatttccg gaagcgagcg aacagtccac aaactgttga acgtggtgtc agcggcggac
      121 agggataagc cctcgtcagc agttccgtca gcactgcgac agcgttcttt acccggtcaa
      181 aacagcgttg cggtgcacca cgcctgacgg atgaactgcg tgctcagggt taccacttta
      241 acgtaaaaac cgtggcggca agcctgcgcc gtcaggggct gagggcaaag gcctcccgga
      301 agttcagccc ggtcagttac cgcgcacatg gcctgcccgt gtcagaaaat ctgctggagc
      361 aggattttta cgccagtggt ccgaaccaga agtgggcggg agacatcacg tacttacgta
      421 cagatgaagt tcggttacat cctgtcagca cggaacctca cgctttttga gcggcacgac
      481 ggatcccccc cacccagtat ccgacagact tttcactgcg ttcgtaaaaa cccttcttcc
      541 ctcccagcat atccactaat tccggtgaca tatcctgcag ataagtctgc aaatcatgcc
      601 ccagatcttc cggtgactgc aactcataaa ccgcgcgcca cacatcaccc ttccttattt
      661 taagccccgg gttaagaata tactgtccgt ggttcagcct cagaaaaagc ttttttccat
      721 aaggattttt gctgttaact tcatgttgag agagctggct gctgatataa ctccttcgtt
      781 tgcggcgttc cggcagcaac gcttcaggaa gatgaaccac acactctgcc agtcgtgccg
      841 cactgaaggc ttccgaaaaa aacagagcac ttcccagatg cgtgtggaac agcgcctgca
      901 tcaggatcac cagaaacatt gccatctgcc ggttgtccag tttgatgagg cgtccttcca
      961 cctgcagtga cagactttca ggtttcagca gggtatacaa ctgcgcactt ttctgttgcg
     1021 cataacgggg cgtactgacc gccgctgaca gaacttgatg ataagccgcc agcccgtcat
     1081 taccggtcag caagggatct gcaccacgct ccagcagtaa ctgaaccagt gcaatattac
     1141 ctgcccgggc tgctgacatc agcggagtaa gattaaacgg cgaacggtga tcaacaccat
     1201 aacgttcaat atcccgcaac acggcaaccg ggttattcat tgagtatggc cggaaatact
     1261 tcagctccag taactgcaga gtcttcgttc tgggctgcct tgtgggctta aaatcttccc
     1321 ggtacagtgc attatacagc gggaacaggg aataaatcag actgtactcc agcgcctgca
     1381 gttgtatggt tttatcggct gtacctgcga ggatatgctg tttccactgt cgtgcatcct
     1441 ctgcggtgat aatctgccat ggcatctcag atgtctgcag gatccggcta cggatggtgt
     1501 ccgcctgctc ctgcttcccc tgtttttcaa gcagatgtgc ggttttctgc cactcatcac
     1561 ggctggaaat ctcttcctgc agattcagag gctcttcctg atgcgtgagc gcaagcagac
     1621 tccacagagg atgctccacc tgctgttcaa caagatacac attgtgttta gcacgggtga
     1681 gcgcaacata cagcgcgttg gtaaaaaatt tatagatttc cgctgaacga tcctgcctgt
     1741 cccgcggacg ggaataacgg gcctcgccct caagatcagc cggtgtaaga ccctcgcaaa
     1801 tatcatcaaa tgcctgccgg gcagcgctta ctatattgta aaggatcacc gtttcgtatt
     1861 ccagcccctt aacctcctga accgaaaaga ccagcggcgt actgaaccag catcgcgcct
     1921 gtgctttctg ttccggatgc atcacaatga ccgctacacg attcgaaagt gaggttttgg
     1981 cattcagttc ctgctttgtc tcttccctat cgtccagcaa ccggatggtc ccttctgcct
     2041 gaccgcagct tctgacaaaa tggtgacttt cccgatcaat agcactaaag cggacctgtt
     2101 tgagtctcag caaccggtta gccagtgcag tgacgtggtg cccgttacgg taattcgcct
     2161 gcaggatatt aacggttgtg tcgtttccct gttgctgccg gaaaaagaga cttttgagcg
     2221 aactccatga gaaaaagctc ggatgaacta tctgattggc atcaccacag agtaaaaact
     2281 gaccgggatg acgtagcgtt ttcagtacca gttgcagttg aacaggcgtc atatcctgaa
     2341 cttcatcaac aaaaatgaca tcataacggg gagttgccaa cgaaagatac tgatgactga
     2401 gcagattact gtcatactga tgcgactgtt tcagccatgc gatataacgt tcaaacagga
     2461 catagaccgt cgggcggtcc tccatgccat agagcgactg ccgtatcccc agtgacagat
     2521 acgcctcccg ggacagcggc ccattcccgg aggcaaccgc accgatcacg cccctgaact
     2581 cctcatacag ggtatgggca gcccccagtg cggcgatagc cctgttgcgg ggcagccagt
     2641 cgctgaacgc actgaaagtc acctccctgc cttccggaat gcgcagagtt tccagaaatt
     2701 ccgtcagcga aagaaagtca atattctgta cctcactccc ttcccctgaa gcgtcataga
     2761 gagtacgcgc tttttccacc agaaatgagg aaaggctgag atacagtata tcaccagcag
     2821 cctgcttcat tttttccagt accagcgccg tcttaccact tcccgccgag ccaacaatca
     2881 ccagcggtag cgggtgttcg tacagtgcct gctgatcatc atcaaaggac agcattttat
     2941 ccaggcggtg gaaacgccca tgagacggat tgatatacgt cagcgcttca gtcgcgatat
     3001 caacaggatc cggcactggt tgttgttgta agcgcccttc atcaatcacc acgtttctgc
     3061 gaagaaaacg tgaagtgtta taggcatgat tgcgaatata ttccagaaca aggcagatag
     3121 tttcaccgtg gtagcgatac agcgaaaaca gtaatcggtc ccggatactg agccgggcgc
     3181 ggtaaagatt ggtgtcaatt ttacgaacat cagcctgaac aaagttatcg gtggcaatag
     3241 cctgtgcaaa ttttttataa cccgggatcg tgtcaggatt cagagtatga taaagacaga
     3301 ttttcatggc tgccggtttc cgtcgtgcaa aaaacatgct gattttacga aggaatgcct
     3361 tactgaacaa ccttgcccgg aatacccccc gaaatcagat ggaacaatca gaaaaatgct
     3421 ctgcagttat ggaataaaga aggtggggtg gtacgccggg agggcgatcc gctggaaatg
     3481 gcgcagtagc tgcaggcctg tcatgatgca ggaatagaag ttcgtgtaca ggttaatgaa
     3541 agcgtcaccc cgtaaataaa ctctgcgctg aaattccttt ttttcattgg agtacctgtg
     3601 ttgttctgag gtgaacatat cacctctgtt caggtagcca aattcagtgt gccacctcaa
     3661 ggttcttagt gagtcgtttt taaaagatat gataatatat cattgtttat gaattgttga
     3721 tgtttaattt agtaaattat atcaaatatg ccatgggttt gctatgttat tcatccagta
     3781 tctggaaata aaatgcacca gctttattgg gttttccttt tgtctctgct tgctaggtgc
     3841 cagaaacttt aaatgttaaa cgaagggggc attttgttca aagccaatca tgttccggta
     3901 ttaatgtatc atcatgtcag tcattgcccc ggactggtta cactttctcc cgtgacattc
     3961 cggaagcaaa taaaatggtt ggctgaaaac aactggaaaa cactgtcatc agatgaactg
     4021 gagttttttt atcggggcgg aaaactcccc cggaaaagcg ttatgttgac atttgatgat
     4081 ggataccttg acaactggtt tcaggtatat cctttattaa aagagtttaa tctcaaagca
     4141 catatttttt taattaccgg ttttattggg aatggtccgg ttcgtcactc tcctggaaag
     4201 gaatattctc atcgcgattg cgaacatcaa atagcgacag ggaatgctga taatgttatg
     4261 cttcgctggt ctgaagtaaa cgaaatgttg caatctggtc tggtcgaatt tcatgttcat
     4321 acgcatactc atacacgctg ggataaaaag ttcagttcac gagaagaaca gtgtaaacat
     4381 cttcggcagg acttgttgag cgggagggaa tatttgaaag aaatgacagg aaaatgcagt
     4441 aaacatttgt gttggccgga aggttattat aacaaggatt atattcaggt cgctgaagaa
     4501 cttggattct attatttata tacaacagaa agaagaatga atgctcctgc taaaggaact
     4561 acacggatag gaagaataag caccaaagaa cgggaaagtt gcgcatggct gaaaagacga
     4621 cttttttatt atacaacccc gtttttttca tcgttgctgg cctttcataa ggggccaaga
     4681 ttaccggatg attaataatg aatatactat ttacggaatc atcaccgaat ataggtggtc
     4741 aggaattaca ggctgttgct caaatgaagg ccctgaagaa aatggggcat tcagttctgc
     4801 ttgtctgcag ggaaaacagc aaaattgctt ttgaagccag taaattgggg attgatatca
     4861 cattcgcgtt atttcgaaac agtcttcaca tccctactgc atggagatta ctcggaatag
     4921 ttcatggttt tcagcccaat gcaatcgttt gtcacagtgg gcatgatagc aatattgttg
     4981 gtttagtacg gttattcact aggaaacatc cattcagaat tatcaggcaa aagacatatt
     5041 tgacacgaaa aacaaaagtt ttttcaataa atcatttttg cgatgaggtg attgttcccg
     5101 gaacaagtat gaagacacat ctggagcagg aaggatgtcg aacccgggtt actgttgtgc
     5161 ctccaggctt tgatttccag aaattatacg ttgattcccg aaacagtttg cctccaaatg
     5221 ttctttcttg gctggcgtcc cgaaggggat gccccgttat tgctcaggta ggaatgttgc
     5281 gcccggaaaa agggcacgaa tttatgttga atttactgtt ccatttaaaa atgaatggac
     5341 gacagttctg ttggttgatt gtggggtctg gttcgcctga actgcgggag catttacagt
     5401 atcagattga cagtatgggc atgcatgatg atgtttttat tgctgacaat gtttttcctg
     5461 ccgcccccgt atatcgggtt gccagtctgg tggttctgcc ttcagaaaac gaatcgtttg
     5521 gtatggtact ggcagaagca tcggcatttt ctgtgcctgt actggccagt cagattggtg
     5581 gaatccctga tgttattcag aataaccaga ccgggacatt gttaccagca ggtaataagc
     5641 acgcatggat gtgcgccctg aatgattttt ttaatgaccc tgggcgtttt tatcagatgg
     5701 ctcgccaggc aaaacaggat atagaagagc ggtttgatat taataaaact gcgttaaaaa
     5761 tactcacatt agcgaagcac aagtaacaat atgttttctg taagtaactt atcatttatc
     5821 ggtttcctta aaaggattgt tttttcctca gattcactcc cggggaagtg ggaacacaga
     5881 aaattccggt tcatgtacat tttgcgatgt gctataaatc cggttgccag tattcgatat
     5941 tattacgaac tgcgttcctt gcagtgcatt gaggatattc tggcaataca acccacgttg
     6001 ccagcaagaa ttcatcgacc ttatttgcat aaaggggggc gagcctggtc ccgggggcaa
     6061 tatattcttg agcattatcg ctttgtgcaa aatttgccag aaaaatattc tgaatttttg
     6121 tttccacaaa aatcggtatc attagtccaa ttcatcggga aagatggaga ggattttgat
     6181 atccagtgtt ctcccagtgg ttttgaccgg gaaggagagt tgatgttgtc attgtttttc
     6241 aataaaatag tgattgcccg gttaacattt tcagtcattc tgactcaaaa tggacacacc
     6301 gcttttattg gcggattaca gggagccccc aaaaataccg gacctgatat tattcggtgc
     6361 gcaacccggg cctgttatgg gcttttccca aaacggatta tttttgaagc attttgtgcg
     6421 ttgatgaaag cttgtaatgt ctctgagtgt ctggcagtca gtgagcacag tcatgttttc
     6481 cggcaattga gatactggta tcagaaacgc aaaacctttg ttgctgtcta cagtgatttc
     6541 tgggagtctg tagcagggaa aacctgtggt gactggtaca aattaccaac acaggtggtt
     6601 cgaaagccgc taagcaatat agctagtaaa aaacgctctg agtatcgaaa acgatacgct
     6661 ttgctggatt atatccatga aactgctatc cgctctttgg atgcatatcc tgtaaactca
     6721 gaacatcagg acttaaatta aggtttgatg tattctctga ataaaatatt aatgatgatt
     6781 ttggtagggg cattcgcact aaataatgaa aaaatacaaa tccgagttta ttcctgaatt
     6841 taagaaaaat tatctttccc ctgtttactg gtttacatgg ttcgttttgg gaatgattgc
     6901 aggtatttca atgtttcccc cttcattcag agatcctgtc ttggccaaaa tagggcgttg
     6961 ggtgggcaga ttgagcagaa aagctcgtcg cagggcgacg attaatttat cgctttgttt
     7021 cccggaaaag agtgatacag aacgggaaat aattgtcgac aatatgtttg ccacagcatt
     7081 gcaatccata gtgatgatgg cagaactggc aattcgtggt ccggaaaagt tccagaaacg
     7141 agtgttctgg aaaggcctcg aaattcttga ggaaattcgg cacaataaca gaaatgtgat
     7201 ttttctggtt ccccatggct ggagcgtgga tattcctgca atgttgctgg cagctcaggg
     7261 ggaaaaaatg gccgccatgt ttcatcagca acgaaatcca gtgattgatt atgtctggaa
     7321 ttctgtacgg cgtaaattcg gggggcgctt acattcccgg gaggatggga taaaaccatt
     7381 tattcagtca gtacgccagg gatactgggg gtattacctt ccagatcagg atcatggtcc
     7441 tgaatacagt gaatttgctg atttttttgc gacctataaa gcgacattac caattattgg
     7501 acgtctgatg aacatcagtc aggctatgat tataccgctt ttcccggttt atgatgaaaa
     7561 aaaacatttc ctgactattg aagttcggcc accaatggat gcatgcattg ccagcgcgga
     7621 caataaaatg attgcccgac aaatgaacaa aacagtggaa attttggtgg ggtcacatcc
     7681 ggaacagtat atctgggttt taaaattgtt aaaaacgcgc aaatcaaacg aagcggaccc
     7741 gtacccttga aattataaag tacaggtatt tccactagtt gtttcttaca ggttaccaat
     7801 cgaaacacat cccccttccg tgtgttgccc gtttggcacg gaggactgtg tagatgcagt
     7861 tcgttaaatt taatttatg
//
LOCUS       DADLTY010000185         7782 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.186, whole genome shotgun sequence.
ACCESSION   DADLTY010000185 DADLTY010000000
VERSION     DADLTY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.186"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="JN334_003557"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="JN334_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HAY7439571.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="JN334_003558"
     CDS             complement(1433..1759)
                     /locus_tag="JN334_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HAY7439572.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="JN334_003559"
     CDS             1894..2235
                     /locus_tag="JN334_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HAY7439573.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="JN334_003560"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="JN334_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="JN334_003561"
     CDS             complement(2833..3543)
                     /locus_tag="JN334_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HAY7439574.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="JN334_003562"
     CDS             3651..3956
                     /locus_tag="JN334_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HAY7439575.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4475
                     /locus_tag="JN334_003563"
     CDS             4155..4475
                     /locus_tag="JN334_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HAY7439576.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            5035..5613
                     /locus_tag="JN334_003564"
                     /pseudo
     CDS             5035..5613
                     /locus_tag="JN334_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(6299..7288)
                     /locus_tag="JN334_003565"
     CDS             complement(6299..7288)
                     /locus_tag="JN334_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439577.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7373..>7782)
                     /locus_tag="JN334_003566"
     CDS             complement(7373..>7782)
                     /locus_tag="JN334_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1682 domain-containing protein"
                     /protein_id="HAY7439578.1"
                     /translation="YPLSLAIKTLIPGYLSGLPSTSREAMELLEALAEAGVTIGNFFS
                     NDLMTAYGHRQMNKRAEAERIAKEQRLQAERMREENMTDAEWQKELQRREQVKAERRT
                     YGESLRSATHSAGRSRAAIVADLESGGNWMDSL"
BASE COUNT         2028 a         1991 c         1880 g         1883 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc ccctatcttc tattgcacta ccgcactaga
     6001 caactagtac aaaacaaagg cccagaccat tggtccagac cgtagttagt taatcacagt
     6061 gacaagccac cacctgaagt cctgctgata ttcagtccag acttacctct gctcatggtc
     6121 ttacctccct cggtctcctg accttgggta tcgactacag cttgctgagc gtcatctgga
     6181 ggagctacag gaggagctac aggaggcggg accgaagccc caccttgagt cccaccacca
     6241 cctgtcaggc ccttggcgat ggacctgaag cctttactaa tgctcttacc cattggtctt
     6301 acgcttcggt cttggtgaac ttcaggcgtt tgactacagg caggacggca gtagctgagc
     6361 cagtgcccat cttgatggtc gtagcagctt cagcgacaac gaacacttta ccgaggattt
     6421 ccaagtcgtg ggactgagag tcgccatcac gtgaaaggac gaccgtaata ttctcaccgt
     6481 cgtgacggaa gcagccgaag ctcagggccg tgaagcgttt agccagcatg aatacaccct
     6541 tgtcttcacc gttgtaatag gcgatgtctg tccccagcat atcgacgata gcgtcttcac
     6601 cggagaagcc ttgcttagca gccagacggg acagtgcttt cagttggctc tcatgagcga
     6661 taataccgta gtcagacacc tcagaaccga aagaagactc tgtgttctca tacagagcgt
     6721 ccatgatagc gtctgcctta gcggtcaggc taccagtcag gacaggaagc tctacggtct
     6781 tcagcagctt agaggattcc agaacgtcga ccatgcgagc gttgatggta cggaccacat
     6841 tctcacagat gttcatagcg tcctgctgaa gtgtgccctg agtgaatccg gtggatgact
     6901 tggcgtccca gagcttggtc tgctcagaga tgagaatagc ctgctcgatt ggttcgacca
     6961 cgatttcttg tccagacgga acggtcacga cttcaggtcg cttgccacca cggacaggaa
     7021 cgaccacaga gcggtcagcc agagtgtgag caatgattgg ggtcggtaag ctgttacggg
     7081 tcggcaggac ttctacaggg aacgggatgt tctcttgagc gtctttaagt acaccgttca
     7141 gcatagcctt aaccgtctta gctaactgga gggcatagac ctgcgggtca gcgttagtcg
     7201 gcagaccttc attgacctga atgttgtcgg tatcggtgat gcgtactggc tcaatgccaa
     7261 actggaaagc gtctttaatc gtagtcataa taatcaatct cctaaattag tttaaatact
     7321 gtgagtcctc aaaactgagg agtccctaac atcgggtcag ggactgtgtg ttttacaagc
     7381 tgtccatcca gttaccgcct gactcaaggt cagcgacaat agcggcacgg gagcgaccag
     7441 cggaatgggt agcactgcgg aggctctcac cataggtccg gcgttcagct ttgacctgtt
     7501 cgcggcgctg tagctctttc tgccactctg catcggtcat gttctcttca cgcatacgct
     7561 cagcctgaag tcgttgctct ttagcgatac gctcagcttc agctcgtttg ttcatctggc
     7621 ggtgaccata ggcggtcatc aagtcattac tgaagaagtt gccaatggtc acgccagctt
     7681 cagccagagc ttccagtaac tccattgcct cgcggctggt agacggcagg cctgacaggt
     7741 agcccggaat gagggtcttg atagcgagcg acagcggata gg
//
LOCUS       DADLTY010000186         7781 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.187, whole genome shotgun sequence.
ACCESSION   DADLTY010000186 DADLTY010000000
VERSION     DADLTY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.187"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            1..1331
                     /gene="yjeM"
                     /locus_tag="JN334_003567"
                     /pseudo
     CDS             1..1331
                     /gene="yjeM"
                     /locus_tag="JN334_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313164.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="JN334_003568"
     CDS             complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="JN334_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY7439579.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRNVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4815..5783)
                     /gene="psd"
                     /locus_tag="JN334_003569"
     CDS             complement(4815..5783)
                     /gene="psd"
                     /locus_tag="JN334_003569"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY7439580.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="JN334_003570"
     CDS             complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="JN334_003570"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY7439581.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7027..7572
                     /gene="orn"
                     /locus_tag="JN334_003571"
     CDS             7027..7572
                     /gene="orn"
                     /locus_tag="JN334_003571"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY7439582.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1762 a         2016 c         2104 g         1899 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcacagtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgatgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt ttgctattca ccgctgaaag
      841 ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc ctgaatgcga
      901 tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc ttcattctgc
      961 tggtttcgtt tggtggcggt accgcatcgg cgttctttaa caagctgacg ctgatggcga
     1021 acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccgttt tttaaagcac
     1081 gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca atgattgcga
     1141 cggtggttgt cgttctggtg gtgacatttg cgaacgtctt caccattatt cagcctgtgg
     1201 ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc ttcttctcgc
     1261 tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa cctgagttag
     1321 ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg agttgtcagg
     1381 aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg atgttaagct
     1441 gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact tcccgcctga
     1501 cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag gctttccaga
     1561 cgcatctgga agggcgggaa tggcatatcg ataccgtggg cgtggaagcc tgccagaatc
     1621 agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc gtaaatacgc
     1681 agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg tgccgggttg
     1741 tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac ttcttcactg
     1801 ttggcatcgg caggggccgg aatcgtcaac actacgcgcg tgaccgagtc agagagcgac
     1861 cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc tttacggtcc
     1921 cagtcgctga tggtggtggc gcgggtgtta atttttgtca cgctaccagt aagatcgcgg
     1981 atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca ggatgatcag gccagagata
     2041 aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc ggcaaccagc
     2101 cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc gccaatcagc
     2161 atcagcagat attttgtgat ggtggtgatg gcgtaacccg tacccggcgt taaatccagg
     2221 tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg cgtggtgatg
     2281 ataaatacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag actttctacg
     2341 ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc gaaagcggaa
     2401 tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa aattgagcgc
     2461 accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc atcgacttca
     2521 attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc acgttgcgct
     2581 aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg ctggatcagc
     2641 atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc aaccgaggtt
     2701 tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac ccaccgccga cgccagaatg
     2761 gcaaccagtg gtgcgccaat catcatgttc cacagcatat ggttggtaat gttgtcgccg
     2821 ctgccttctt tgttgagata caacggaata ccggcttttt tcaggcttag ggtgaccacc
     2881 gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga aaattcgcgg
     2941 tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc cgatgctcat caggtagtag
     3001 cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat gagcgataaa caagccgttc
     3061 gggcgggcaa aggtggcgca aatcatcact acccacagca gcggcacggt ggccgttaca
     3121 ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc gtaacccagc
     3181 gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg accagaaaag agtgcgcaac
     3241 gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg ttcaagaaaa
     3301 cgggtgaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc gccaaacagc
     3361 ggcaaaattg tctctttgct ggtcagcatc atcacactgg ctttgcccaa ctgactgaag
     3421 gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca ggcgatggtc
     3481 atcgggcgca cgtcagaggc ccagaacaga tagcggtgcg ttgcttcgtt cacctctttc
     3541 agcgcatcct ccagttgcct gttggagact ttcagcttgg tcagttccaa tagtagcgtg
     3601 tcgccaccct gcaacaatga gttcagcaac tcacgctgag tgcgcagttg tgcttccaga
     3661 atacggtttt gctcggcggt cagcggctga ccgtcggcct gatggatttg ccgtagcagc
     3721 ggctgtttat taagtaaatc ctcataacgt aaccgttgca cacgcaactg cgccatttcg
     3781 gtatcaagct gttgtggttt tggcatttct ggtagccgtg ccacctgcgc ccgcagcgct
     3841 tcgccgagca ggttggacga tcccagccat tgcgactgtt cacgcagcgt attcaacgcc
     3901 tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac gagctccatc
     3961 cgctgcgcct gttgattcag cgctgccgat agttcgcggt taattttgaa ttgcgcgacg
     4021 atatctttcg gcaaatcggc actgttttct gccagtaatt cggtactttc cagcgcccgc
     4081 tccgcctcaa gctgacgttg gctattcaac tgattacgta aggcctgcaa atacgcatcc
     4141 agttgctggc tctctttttc cgccagctct gagcgtaagc gcgctaattc ctggcggtta
     4201 ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg tgcagagtca
     4261 gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag cgttcccagg
     4321 cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt gctgttgcgg cagttgattc
     4381 agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct gggcctgacg gcttttatcc
     4441 agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga cattcccggc
     4501 gacacgttgc gcggctcgtc acgaatgttg tttaattgtg cgcgcagagt agcggagagt
     4561 ttcggataat tatcgataac ttgctgatat tgtttgatgc gctcaaggga accttttcgt
     4621 tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg tgcgggtttc
     4681 gccgcttttg cctgctccag ttcctgagtg atttgtttgc tatcgggggc cgtcgcggcg
     4741 tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg cacgtcagcg
     4801 tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct ggcgtcgtgt
     4861 tctgcttcga tctcttcagc aggaagtggg gcgggttcag cgtctggcgt aacaaaggtt
     4921 tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc cagttgctca
     4981 accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag tttaaagcga
     5041 cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc aggccaggtc
     5101 cagcgcttga tgataccttc gcgcggcggc gtaatggtgc ccgcccagac cgtctcaatg
     5161 ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg ggccaaattc ggtatcgaaa
     5221 aggcaaatca cgcgttcgtt acgggcaaac agatttggca cgttctgagc ggtgagatgg
     5281 ttaacggaaa agagatcgcc cggcacgtag atcatctcac gcagaatacc gttgcacggc
     5341 atgtgtacgc gatggtagtc acgcggggag aggtaagtgg tcacaaacgt accgttgcgg
     5401 aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta gttgtggcct
     5461 ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac gccatcggca
     5521 ggcatgacca gtacattcgg atcggtatcg attgggcgta cttcgtcacg cagcggacgg
     5581 acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc ctctttcatg
     5641 tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca tcctgcccgc
     5701 ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat gtactgtagc
     5761 gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg ttcctgatcc
     5821 ggccaacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta gcttaattgt
     5881 cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct ttccagaata
     5941 cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc ttcccggata
     6001 gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc taaatagtca
     6061 tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag gccgaactca
     6121 cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag ccgagcggcg
     6181 gtcgtggtgt gctgaccgag ccccgagttg tcagagacat cgttggtcag gatctctttt
     6241 tgcagcccca gcaacgcgtt cagcaggctg gatttaccga cgccagactg cccggcaaaa
     6301 atgctgatgc gcccggtcaa cgcctcttcc agcggtttca gcccatcctg agtatggctg
     6361 gaaaccatca atacgcgata accgatgttg cggtagatat ccatctgctc gttgacgaac
     6421 gccatgcctt cgtcgtccag caggtctatc ttgttgagca cgataatcgg ctcaatctgc
     6481 aaggtttcgc aggccaccag gtaacggtcg ataatattga gcgacagctc cggcaaaatg
     6541 gcggagacaa tgacaatctg gtcgatgttg gcggctatgg gtttcacgcc gtcgtagaaa
     6601 tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt ccacgatccc tttgacattt
     6661 acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt taccagcgaa
     6721 cggatggtac ggcgaatatt gcagcggtga acgtcgccat cggcggattc cacatcagcg
     6781 tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag attgtcgtcg
     6841 tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt cacgcggcgc
     6901 tgctggcctt tggagagttt atttttactc aatcgtactg actcctggtc gcccctgatg
     6961 ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga atgggtggaa
     7021 aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat gaccggtctg
     7081 gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc caatctgaat
     7141 attctggcag aagggccgac cattgcagta catcagtccg atgaacagct ggcgctgatg
     7201 gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt gaaagcgagc
     7261 acgatgggcg atcgggaagc tgaactggca acgctcgaat tcttaaaaca gtgggtgcct
     7321 gcgggaaaat cgccgatttg cggtaacagc atcggacagg accgtcgttt cctgtttaaa
     7381 tacatgccgg agctggaagt ctacttccac taccgttatc tcgatgtcag caccctgaaa
     7441 gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca ggggacgcat
     7501 caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg cgagcatttt
     7561 atcaagctgt aaaatttttc ctggtcacgt aagcgccgga atccacttgc ggcggcgtga
     7621 ttttgacgct aaatcgaacg tcttgctgat aatttcagca cttgagataa aaacgcaaaa
     7681 aaaacttttt tggggggttg cagagggaaa gatttctcgt ataatgcgcc tcccgtaacg
     7741 acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt a
//
LOCUS       DADLTY010000187         7744 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.188, whole genome shotgun sequence.
ACCESSION   DADLTY010000187 DADLTY010000000
VERSION     DADLTY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.188"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..1015
                     /locus_tag="JN334_003572"
     CDS             <1..1015
                     /locus_tag="JN334_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7439583.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1104..2564
                     /locus_tag="JN334_003573"
     CDS             1104..2564
                     /locus_tag="JN334_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY7439584.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="JN334_003574"
     CDS             complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="JN334_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HAY7439585.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2981..3667)
                     /locus_tag="JN334_003575"
     CDS             complement(2981..3667)
                     /locus_tag="JN334_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7439586.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="JN334_003576"
     CDS             complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="JN334_003576"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HAY7439587.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4639..6684
                     /gene="dcp"
                     /locus_tag="JN334_003577"
     CDS             4639..6684
                     /gene="dcp"
                     /locus_tag="JN334_003577"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HAY7439588.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6729..7247)
                     /locus_tag="JN334_003578"
     CDS             complement(6729..7247)
                     /locus_tag="JN334_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HAY7439589.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7523..>7744
                     /gene="ydeI"
                     /locus_tag="JN334_003579"
     CDS             7523..>7744
                     /gene="ydeI"
                     /locus_tag="JN334_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HAY7439590.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHK"
BASE COUNT         1941 a         1813 c         1955 g         2035 t
ORIGIN      
        1 catcttgctg gtgacgctgc gtattattca gggattgggt gcaggtgcgg aaatttccgg
       61 tgccggtacg atgctggcgg aatatgcgcc aaaaggtaag cgcggaatta tctcctcatt
      121 tgtggctatg ggaactaact gcggcacctt aagcgcaacg gcaatctggg cctttatgtt
      181 cttcattctc agtaaagagg aactgctggc gtggggatgg cgtataccgt tcctggcgag
      241 cgttgtcgtg atggtctttg ctatctggtt gcgtatgaat ctgaaagaaa gcccggtctt
      301 tgagaaggtt aacgacagca accaaccgac agcaaaacct gcacctgctg gtagcatgtt
      361 ccagagcaaa tccttctggc tggcaacagg gctgcgtttt ggtcaggcgg gtaactccgg
      421 tttaattcag actttccttg caggctattt agtacagacg ttattgttta acaaagcaat
      481 tccaacagat gcattgatga tcagttcgat tctcggcttt atgaccattc cgttccttgg
      541 ttggttatcc gataaaattg gtcgccggat cccgtatatt attatgaata cctctgcgat
      601 tgtgctggcg tggccaatgc tttctatcat cgtagataaa agctatgccc cgagcaccat
      661 tatggttgca ctgattgtga ttcataactg tgcggtgctg ggattatttg ctctggaaaa
      721 cattaccatg gcagaaatgt tcggctgtaa aaaccgcttc acccggatgg ctatttcaaa
      781 agaaattggt ggtcttatcg cttccggttt tggtcctatc ctggcgggta ttttctgcac
      841 catgacggaa tcctggtatc cgatcgccat tatgatcatg gcatattcag tgattggttt
      901 aatctctgcg ctgaaaatgc cagaggtgaa agaccgtgat ttaagtgcgc tggaagacgc
      961 tgcggaagat caaccgcgtg ttgtaagagc tgcgcaacct tccagaagtc tgtaaaccct
     1021 caatcccttc tcttaccgga gaggggattt ttattcatat aacaaaacat atagcttgcc
     1081 atatttaaat ttaaggaatt gttatgggaa ataatttgtt atcagcaaaa gcgacactcc
     1141 ctgtttatga tcgtaataac ctggccccaa gaattgttca tttaggcttt ggtgcatttc
     1201 accgtgcgca tcagggcgtg tatgccgata ttcttgctac agaacatttc agtgactggg
     1261 gatattatga ggtcaactta atcggcggcg aacagcaaat tgccgattta caacagcaag
     1321 ataatcttta taccgttgcg gaaatgtcgg ccgatgcgtg gacggctcgc gtcgttggcg
     1381 tcgttaaaaa agccttgcac gtacagatgg atggcttaga aaccgtgttg gctgcgatgt
     1441 gtgaaccgca aatcgcgatt gtctctctga caatcaccga aaaagggtat ttccactctc
     1501 cggcgaccgg acagttaatg ctcgagcacc cgatggtcgc tgccgacgtg caaaatcccc
     1561 accagccgaa aacagcaaca ggggtgattg ttgaggcgct ggctcgccgt aaagcggcag
     1621 gacttcccgc atttaccgtc atgtcatgtg acaacatgcc agaaaacggt catgttatgc
     1681 gtgacgttgt cacttcctac gcgcaagccg ttgatgtaaa actggcacaa tggatcgaag
     1741 ataacgtgac tttcccatca acaatggtgg accgtattgt gcccgcagtg acagaggata
     1801 cgctggcgaa aatcgaacaa cttaccggtg tgcgcgatcc tgctggcgtt gcctgtgaac
     1861 ctttccgcca gtgggttatc gaagataact ttgtagccgg acgtccggaa tgggaaaaag
     1921 cgggagccga actggttagt gatgtgctgc cttatgaaga gatgaagttg cgcatgctca
     1981 acggcagtca ttcattcctg gcatatctgg gttatcttgc tggatatcag cacattaatg
     2041 actgtatgga agatgaacat tatcgtcatg cggcgtatgg catgatgttg caggaacaag
     2101 cgccgacgct gaaagtgcag ggcgttgatt tgcaagatta cgctaaccga ttaattgcac
     2161 gctatagcaa cccggcgtta cgtcatcgaa cctggcagat tgcgatggat ggcagccaga
     2221 aattgccaca gcggatgttg gattctgttc gctggcatct ggcgcatgac agcaagttcg
     2281 atctgctggc gctgggcgtc gcgggttgga tgcgttatgt cggtggtgtt gatgaacagg
     2341 gaaatccgat agaaatcagt gacccactgt tacctgttat tcagaaggct gtacaaagta
     2401 gtgccgaagg gaaagcgcgc gtccagtcat tgctggcgat taaggcaatc tttggtgatg
     2461 atttgccaga caatagcttg tttactgcaa aagtgacgga agcgtacttg tctttattag
     2521 cgcatggcgc gaaagcgacc gtggcgaaat attccgtgaa gtaaaaaaag caatgccgcc
     2581 atcggcggca ttcacacact tagttcatgc cgagacgaat caggtccaga ggtgccagtt
     2641 tctcttcaca accttcaaca actacggttt ttccgcggcg gatttgctca gcggtcagac
     2701 cttcagtatc aatcgacagg atcttgccag tgtgaccggt gccgctaacc ataacacggc
     2761 tgccagtggt gattgcgtta cggttacgat cgtaggtcgt cataggtgtt ttctcctttc
     2821 tgatttacag tcgaagccca cgtaacgggc gcaatataaa tactcctgtc gagtgaaata
     2881 ttttttgttt ttgatcaagc tcacatcttt tttgatgcat gcatgagccg taaggcgggg
     2941 ggaaaagcct ctcatcccgg atgggatgag agtaaatgaa ttactcttcg ctaaaccagt
     3001 cgctgttttc ctggcggatc tggcgcacgg attcgctgat ttcctgcaaa tgttgcgtca
     3061 ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc agagaaaata tcaagatgct
     3121 ggcgtaacag catttctggt ggagaaacat ggtcgaaact catatagcgc acgcgatcaa
     3181 cggtcgcttt caggttctca atggtatccc acgccagttg acagtcggca atctgcgtca
     3241 ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc cagttgcttg cgctcaatgg
     3301 caatgcgttg ctggtgaaga ttttgttcca gttggtagca ctggctttcg gtaatcatgc
     3361 tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg gataaaactg ccgttgcgca
     3421 cctgggccat ggaaattttg ttgacgtagc tgccacgctg cggacgaatt tgaatcaggc
     3481 cgttttccgc cagtttaata aaggcttcac gaaccggctg gcgtgacaca ttgaaacgaa
     3541 cagaaacttc tttttccgac aacggtgtgc ctggtgcaat caggcaatgg acaatgtcgc
     3601 gacgaagaat acgataaatc tgctgattga caggctgtgt gggattaagt tgcgtttcga
     3661 cggtcattcg ttcttgcttg tgagtgagtt aactgcatga gtctaccact tttttgtgac
     3721 aagcaataac ccggcagtta cgccgggtat aaaaattact gacggtggac attcagtccg
     3781 gcatagcttt gggtaaccgg catcatttcc agggtattga tattgacgtg agcaggcagc
     3841 gttgacaccc accagacggc ttcgctgaca tcttctggcg tcaatgcaac ggtattttga
     3901 taggtttttt ccgctttacc gtcatcgcct ttaaagcgga cattggaaaa ctcggtgcca
     3961 cccaccagac ccggttcgat gtcggtgacg cgcaccgccg taccatgcag atccgtacgc
     4021 agattcaggc taaactgacg aacaaacgct ttcgtcgcac cgtaaacgtt accaccggca
     4081 tacggccagc tgcctgccgt tgagccaatg ttaataatat gaccatgatt acgttcaacc
     4141 atacccggta agacggcgcg cgtcatatat actaggcctt tgttgttggt atcaatcatc
     4201 gtttcccagt cttcaacgct ggctttatgc gcaggctcca tgcccaacgc caggccggca
     4261 ttatttacca ggatatcaat attgctccac tcggcaggga gcgatgccag catctcttca
     4321 atagcggcgc ggttgcgaac gtccagttgg gcgatataca gattatctcc cagttcgtct
     4381 gttaactcct gcaaccgctc ctggcgacgg ccagtggcga taactttatg cccttgttga
     4441 ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg ctccagttac taaaacgatc
     4501 atctcactgt tcctcaacgc ttttgtgtgt caataacata gcacgtgatg cttaagagcg
     4561 ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa gcctacgcta tggaacacct
     4621 cgcgttcagg agaagaaaat gacaacaatg aatcctttcc ttgtgcaaag cacactgccg
     4681 tatctggctc ctcattttga tcaaattgcc aatcatcact atcgcccggc attcgatgag
     4741 ggaatgcaac aaaagcgggc agaaattgct gccatcgcgc ttaacccgca aacgcctgat
     4801 ttcaacaata ctattctggc actggaacaa agcggagaat taattacccg cgttaccagc
     4861 gtcttttttg cgatgactgc ggcgcatacc aatgatgaat tacagcgtct tgatgaacag
     4921 ttttccgctg aactggcgga actggctaat gatatctatc tgaacggtga attattcgcg
     4981 cgggtagatg ctgtctggca gcgccgtgaa tccctggggc ttgatagtga atccatccgc
     5041 ctggtggagg ttattcatca acgttttgtc cttgccggag ccaaacttgc acaagctgat
     5101 aaagcaaaat taaaagtact gaatacagaa gctgcgaccc tgaccagcca gtttaaccag
     5161 cggttacttg cagcaaataa atccggcggt ctggttgtga acgatatcgc gcagctggca
     5221 ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg ctcgcgagaa aggtctggat
     5281 aacaaatggc tgattccgct gctgaatacc acccaacaac cggcgcttgc cgaaatgcgc
     5341 gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga cgcgagcgga aaaaaatgat
     5401 gccaatgata cccgcgctat cattcaacgt ctggtagaga ttcgcgcaca gcaggcgaaa
     5461 ctgcttggtt ttcctcatta tgccgcatgg aaaatcgccg atcagatggc aaaaacgcca
     5521 gaagcagcac tcaactttat gcgggaaatt gttccagcgg cgcgtcaacg tgcgagcgat
     5581 gaattagcct ccatacaggc ggttatcgat aagcagcagg gcgggtttag cgcgcagccg
     5641 tgggactggg cattttatgc ggaacaggta cggcgggaga aatttgatct tgatgaggcg
     5701 cagctcaagc catattttga attaaacacg gtgttaaatg aaggtgtatt ctggaccgcg
     5761 aatcagctct tcggtattaa gtttgtcgaa cgttttgata ttccggtcta ccatcctgac
     5821 gttcgtgtgt gggaaatttt tgatcataat ggcgtgggac tggcgttatt ttacggtgat
     5881 ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg gcaattttgt tgagcaatca
     5941 acgcttaatg aaacgcatcc ggtaatttat aacgtctgca attatcagaa acccgctgcc
     6001 ggtgagcctg cgttgttact ctgggatgat gtaataacct tattccatga atttggtcat
     6061 acgctgcacg gcctttttgc ccgccagcgt tatgccacgc tttccggcac caacacgccg
     6121 cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact gggcaacgca tccgcaggta
     6181 ttcgctcgct acgcccggca ttatcagagc ggggcagcaa tgcctgacga actgcaacag
     6241 aaaatgcgta atgccagcct gttcaacaaa gggtatgaga tgagcgaact gcttagcgcc
     6301 gcacttctcg atatgcgctg gcattgcctg gaagaaaacg aagcaatgca ggatgtcgat
     6361 gattttgaat tgcgggcgct ggtggcggaa aatatggatc ttcctgctat accgccacgc
     6421 tatcgcagca gttatttcgc ccatattttt ggtggcggat atgctgcagg ttattacgct
     6481 tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt ggtttgttga gcagggcgga
     6541 ttaacgcgtg aaaatgggca gcgttttcgc gaggcgatcc tttccagagg taacagcgaa
     6601 gatctggaac gcctgcatcg acaatggcgt ggtaaggctc ctcagattat gccgatgctg
     6661 caacatcgtg gcttgaacat ataaacacca ccaatggctg gcctggagat atcgccaggc
     6721 cagcctggtt atagcagtaa ttgcagcagc tgggcgaggg caaacctcac cgctaaagct
     6781 aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag tgtagttctg gcctttaata
     6841 tgccacgcga accagacggt acccgctggc gtaccatctt caccgccctc cggtccgccg
     6901 tagccggtaa tggcaatact gacatcagca tccgcacgct ctatggcacc tgttgccatt
     6961 tctgctgcca ctttctcact caccgcagaa tatcgttcaa gagactgctg gcttacgctg
     7021 aggattttca tctttgcctg atcggtgaaa gtaacaaagc ctgcaccgta aaatttgggt
     7081 gtatcttcag ctgcacacag ggcgctagcc aacttaccgc cggtgcatga ttcggcggta
     7141 gttaagcgta attgatccgc gataagtctt tgactcaacg cggatgtcag gttttcaata
     7201 gtgtcggtat ctgcgagttg tacaatttta tctttattaa tgttcactgt tttttgtcca
     7261 cgctattata tgtaaaggtg atttgctttt tctgtatgct cgtccaatat aataattttc
     7321 aaacatataa atccgattat tctgaaaaca tgtttaagac tcttcaatct tcttaacttt
     7381 agtgagtcaa attctctgaa tatttgacag ctagtgggct gattttaaat gccttataac
     7441 aattggcagt attcttattt ggcaattata aaaatagcaa ccagacttaa taagtcaaac
     7501 caaacgcaat taaggagtta gtatgaaatt tcagtccatt gtattagcaa gttttcttgt
     7561 catgccttat gcgttggcag atgaccaggg cggtttaaaa caggatgcag cgccaccgcc
     7621 gcctcatgca atagaagatg gctatcgcgg aaccgatgat gcaaaaaaaa tgaccgttga
     7681 tttcgcaaaa aacatgcacg atggcgcttc ggtttcacta cgcggtaatt tgatttctca
     7741 caaa
//
LOCUS       DADLTY010000188         7619 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.189, whole genome shotgun sequence.
ACCESSION   DADLTY010000188 DADLTY010000000
VERSION     DADLTY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.189"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..349)
                     /locus_tag="JN334_003580"
     CDS             complement(<1..349)
                     /locus_tag="JN334_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAY7439591.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAF"
     gene            complement(346..1344)
                     /gene="fecC"
                     /locus_tag="JN334_003581"
     CDS             complement(346..1344)
                     /gene="fecC"
                     /locus_tag="JN334_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HAY7439592.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(1341..2243)
                     /gene="fecB"
                     /locus_tag="JN334_003582"
     CDS             complement(1341..2243)
                     /gene="fecB"
                     /locus_tag="JN334_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HAY7439593.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(2288..4612)
                     /gene="fecA"
                     /locus_tag="JN334_003583"
     CDS             complement(2288..4612)
                     /gene="fecA"
                     /locus_tag="JN334_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HAY7439594.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(4699..5652)
                     /gene="fecR"
                     /locus_tag="JN334_003584"
     CDS             complement(4699..5652)
                     /gene="fecR"
                     /locus_tag="JN334_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HAY7439595.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(5649..6170)
                     /locus_tag="JN334_003585"
     CDS             complement(5649..6170)
                     /locus_tag="JN334_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAY7439596.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            6446..6650
                     /locus_tag="JN334_003586"
                     /pseudo
     CDS             6446..6650
                     /locus_tag="JN334_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6644..6748
                     /locus_tag="JN334_003587"
                     /pseudo
     CDS             6644..6748
                     /locus_tag="JN334_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            7088..7345
                     /locus_tag="JN334_003588"
     CDS             7088..7345
                     /locus_tag="JN334_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HAY7439597.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT         1736 a         2026 c         2113 g         1744 t
ORIGIN      
        1 caaaggccag cagcggcagc accatcacgg gcagtgacgg cataagaagt agagccccca
       61 cagaggccag gctggcggca tggttaacgc cgagaatatc cggtgatgcc agagggttgc
      121 gcacaatccc ctgtatcagc acgcccgcca cggcgagggc tgcaccgaca aacagtgcca
      181 gcagcaagcg cggcagtcgg tactccatca atacataata atgctcgcgt ccggcctgcc
      241 agtcggtcag cagcgcgcgc cacggcacgg ggatcactcc catatggagt gataacagcg
      301 cacagcccgc cagggcaagg gtgatgaaaa taaccagcgc aattttcatc ctcgcctcct
      361 cacaagccag acaaagcaag ggctgccaat cagcgccagc actgcgcctg cgggcagatc
      421 tccggggaag gccagcgcgc gtgcgagtac atctgccagc agcatcagcg tggcccccag
      481 cagcatgctc actggcagta cgttgcgctg atcgaagcct gcccagaagc gcgccagatg
      541 tggcaccagc agaccgataa acgccaccgg acctgccacg ctgacgcacg caccaaccag
      601 aagcagcact aacatattga tgaccaaacg tagcctcgtc aggttcactc ccagcgtatg
      661 ggcggtgctg tcgctgaggt tgagcaggtt cagttgattc gccagcagta acacgacagg
      721 gactgcagtg accaccaccg gcaagagctg ccagacatcc tgccagcggg cgtgggacac
      781 tccgcctgcc agccagtaaa agatgccgta agcatgatct tcggccagca gcagggtgat
      841 gcgggtaagg cccatacaaa aggccgacag cgcgataccc gcgaggatca gtttgtttct
      901 gtcatgggta tgacgaaatc cgcctcctgc ggtcatgacc agcagccagc tcacgccgcc
      961 cccgcatgcc gcgatgaacg acagagaata gcctgcaatc ggcgtcggac tcagcgcgct
     1021 ggtaagcgcc atagccagcg ccgcgccgct gttaatgccg agcagtgaag gagaggccat
     1081 tgggttgtgg gtcagggttt gcagcagcgt gcccgcgagc gccaggcttg cgccgatcag
     1141 aacggcgacc aggcttcgtg gcaaacgaag gttttgcacc agcgcttctg gtagcgttgg
     1201 cgtgtgtcca ggcagcaggg cgcgggttgc atctgctccg gaaacaggaa tggccgagta
     1261 gcaaaacaga ctcagccaga aaataataat aagtgctgca acgggaagcc cccacagcag
     1321 catcgggtgt tttatcgcgg tcatttcaca acggtaagcg gctgatggtg gaagattttt
     1381 accgtgtcag cggcaatacg ctctgcagca aaaataccgc gcatccgcgc ccaggtgtta
     1441 ctgtcgaccg aagcaacctg ctgcttctgc gcggcggtta acatctgcca gagcggatct
     1501 tgttgccagc gtttaacaat gctctcttcg cgatagtggg caaccagcag ccaggcagga
     1561 ttgaccgcca gcagttgctc caggccgatg gacggcatgg acgaacccgc catcgcagcg
     1621 ggaacgttca gccccagaga ggccagcacg ctgccggtcc aggtctcctg agtatgcagg
     1681 ttgaattgct gttcgcgtga tgtgccaaag gccacgcgtg tccctttggg aagctggctg
     1741 gcccactgcg ccatcctctc tttatgttgt tccagacgtg cctgcatctc tcgcttttta
     1801 cccaccattt cgccgatgat agccgcagat tgcaaatttt cagcgtaggt ttcgttgcgg
     1861 gacttaagca gcagtaccgg cgcgatttgc tgcaaggcga tgtaaacccc cgcatggcga
     1921 ctgctgtcgg caatgatcag gtctggtttc agagcggcaa tggcttccag gctcggctgc
     1981 gcgcgcgttc cgacggactg ccacggtttc aggtgcgcac gcacttcggg caggatgcgt
     2041 tttgcatcgt tatcgtcggc aataccgatc gggctgacgt ccacggcggc cagcgcatcg
     2101 gcgaacgaga gttccagcac cacaatccgt tgtggcgttt tttcgagtgt aaacgtgccg
     2161 tgttcgtcct gaaccatggc ggcaaaggcg tggctgatca ccagcagcag gcctgcaaaa
     2221 agaaaacgga taaatgccaa cataatcaca ttccagctaa aagcccggca agccgggcgt
     2281 taacacatca gaacttcaac gacccctgca tatacagcgt gcgcggctgg cctgcataga
     2341 tgcctttgtt gttgtcgtca taagagcgga tgaagtagtc ctggtcgaag atgtttttca
     2401 caccgaatgc caggttcaga tctgccatct gcgggccaaa gtcatacgcc acgcgtgcac
     2461 cccagagcat gaagccggga atgcggccgg tactgccgtc ggcgctctct ttcaccgtat
     2521 tggcgttatc cgcaaactgg ctggactgga aatcgctgtt cagattgaac gtccagtttc
     2581 ctggcttgta gtccacgccc agcgtgcctt tatgtttcgg ggagaatggt accagattgc
     2641 cgtaggtgtc gcctttctcg cggatttccg cgttcacata cgcatagctg gcgtagatgg
     2701 aaacgttatc aagcgttggc gttagcgtac ccagatcgta acgtgcctgc gtttccagcc
     2761 cggtatggcg cgttttgcca cgtgcagtga cggtgtcgtt ggtctggttg gagtcgtact
     2821 gattgttaaa gttaatcagg aacagcccca tttccgccgt cagcgcgccg tcgtcgtagc
     2881 gggtaccgag ttcccaggtt cgcgcttttt ccggttccac attgccgctt tgcacagcct
     2941 tgccaatctg gctgtactgt acggtgccga acgagccttc agtgtttgca taaagattcc
     3001 agctgtcagt caggtgatag agcacgttca acgccggaag cggtgcgtta tagctcactt
     3061 cttcgtgcgt gcctgtgatg gcgttgttct ggtatgactc gatatgttcg aaacgcatac
     3121 ccggcgtgat ggtccagttg ccgatgtcga ttttgtcatc cagataccag gcgtgcgcct
     3181 cggtgccgga acgcgtatcg cggtcgtaag ggcttgagcc ggacggcaac tgcccgctgc
     3241 tggtggcggt gtagtaacgc atttcatgcg ttgattcatt cacatagcga tagcccacgc
     3301 ccacttcgtg cgcggaaggg ccgatcataa agatctggct gtagcgtggc tcaataccgc
     3361 gcacccagta gttacgcggc gagagggtga tgcgtttgcc ttgctccagg tagccgctgc
     3421 gcagggtttg ggtgtagaac ccctgaatgt tgaatttatg ctggctgtct ggctggaact
     3481 ggtagcccag gctcgccagc ttgcgacgac cccagaagcg gtcatacggg cgggtggatt
     3541 gccagcgatc ggcgtcgtaa tccgcgcgag acaggccacc gggcatgtcg gcttcaccgt
     3601 cgtaatattg cagcaggctg ttgaaggtgt gcacctcatc cggcgcatat ttgcttttca
     3661 gcatcaggtc gtcgatgcgg gtggcgctgt gctcgcgcca gtcactgccg cgcgtgccgg
     3721 agtagagcag cgcggtgcca aaaccgttgt ccgctgtgcc gcccaccatc aggttgtgcg
     3781 tctctttcgg gttgttttgt gaagaggttg ggctgagctg accttccacg cccgcctcga
     3841 taccaaagtc ctgcggaatg gcacgggtaa caaagttcac cacgccgccc acgctctgcg
     3901 gtccgtaacg caccgcacca ccaccgcgta ccacgtcaat ggcatccatg ttgccgagcg
     3961 aaatgggagc cagtgaaagc tgcggctgac cgtaaggggc gaaggggacg gggatgccgt
     4021 ccatcaggac ggtcgagcgg ctggcgaggc gcgggctcag gccccggatg ccaaagttca
     4081 tcgccaggtc gtggctgccg gtgccgttgt tttccggcgc gctgacgcca gggatgcggt
     4141 taagtacctc acgcatggtg gttgcgccgg ttttggcgaa atcctcacgg cggatcacgt
     4201 cacgcgcgcc agcatgttca aatacgtcgt tttcacgcgc atcacccagc cagtcgccga
     4261 ccacggtcag ggcatcttct tttggtgcgg gcgcgggctc cagcgtccag ctgttatttc
     4321 ccagcggttt tacctgcagt ccgctgccgt ccagcagttg ttgcaggccg ctctcgacgt
     4381 cgtaatcgcc gtgcaggccg ttgctctgct tgccgcgcgt caggctggcg tcaaccgaga
     4441 gggtaaatcc gctgtgtgcg gcatactgat tgagcgcttt atcgagcgat cccggtgcga
     4501 tattaacctg tgcagcaaaa gcggaaaacg agagaccggc cagcggcagc aggctcaggc
     4561 gaatggcgtt aaccaaaggt gttgttttac gaaaaacgcg taacggcgtc ataccttccc
     4621 catcatcatt tttgttgtgt tcagctatga gtcgaacgag aagggtaaaa aggacaatcg
     4681 aaataagaat tattttcctt acagtggtga aatgtttatc cagtaccgcg taatagactg
     4741 aattttaacg ggaagcgttt gcgcgataac gttcaggatc gcatcggtat ttttcagcgg
     4801 gaacgtcccg ctcaggcgca gcccggcaac ggcgggatcg cagcgcagca cgccgttgcg
     4861 gtaacgggtt agcgtggcta tcacctcacc cagcggttta tcgctgaagc tcaggatgtc
     4921 cttcgtccag cttgtactct cgtcatccag cggtttcact gcgccaaact cagaggcgct
     4981 gaactgcagg ctttcaccag cgttcacgat gcgtttttgc gcgggggcac tggcgagaag
     5041 cacttccaca gcgtgctgct gaacgtcaag ctgcgtgaaa ttatcctgct ggcggacggt
     5101 aaattctgtc cctaaagcag tgagctggcc ctgacgggtc aggacacgga aggggcgttg
     5161 cagggcatct ttcgcggtag taatggcgat ttcaccgtac cagagccgga cggtgcgctg
     5221 atgcgcatca aaacgcacat ccgccgcgct ttgggtattc agcgtgagca gggagccatc
     5281 ttccagttgc tgacggctga ctgtgccttt ggcggtgcgg taatctgccc gcagaccttc
     5341 gccggtttcc gactgccaga gctgccagcc tccgccagcg ccgagcaaca gcagtaatcc
     5401 tttcatcacg tgacggcggg tgaggcgggt atcgtgcaac gcccggctgg caacgtcgcc
     5461 aggcacaccg ccaagctggt tgcgcaggtt ttcaacctgc tgccaggccc actggttatc
     5521 ctgatcctgt tcataccact gttgccagcg cgcttcctgt tgtgggctga cgcgctcgcc
     5581 gcttagcacg gcataccagt gggaagctga acgcagcgcc tgacggcggg aatcggttaa
     5641 caaaggattc ataacccata ctccagacgg aacagcaggc agtgctcgac ggctttcgcc
     5701 acgtattttt tcacggagct gatggaaaca ccgagtttgt gcgcaatctc gctgtatgtc
     5761 agaccatcca gttgcgaaag cagaaacgct tcacgtgttt tgccgtttag cccgtccagc
     5821 atgctgtcga ggagttgtag ggtctcgagt tggctttcgc gttcctcagg tgaaggcgct
     5881 cccccctccg gcataagcgc cagcatctcc agatacgctt tttccagcgc gtttcggcga
     5941 aacaggtcca ccatcacgcg tttggcgata gtgcagagga aggagcgagg atcgcggatc
     6001 gtcgagagcg tttcgctgac cattacccgc aaaaaagtgt cctgggcaat gtcatctgca
     6061 tcaaaagcag actggagttt gcgcgtcagc cagcttttca accagccgtg atgtgtgcca
     6121 taaagcgact cgaacgttaa ggaagctgtg gtagtggcgc ggtcagacat gcggagtgca
     6181 tcaaaagtta attatcacgt agtcatatta atatgagaat ggttatcatt acaattggaa
     6241 ataaaattgt ttccaataga catttttaac atgttgtttt tctaagtgtt ataaggtagg
     6301 tataaaatgg gatggagcct ctgcttctga catgtgtcgg tcagaatgac tcatgatgtg
     6361 gtctgctatt attgacatcc tcactgccct aaaggatggg gatttcggta atgatcttac
     6421 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
     6481 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
     6541 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
     6601 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
     6661 tatcgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
     6721 acaatttgaa aatcataatc tcacttaaaa cggcatccat tttacaaaag atggccgtct
     6781 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
     6841 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
     6901 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaaa
     6961 ccattagtct gcttcctgtt cttacaataa tcagtattat gtattttatt tatatagcaa
     7021 aatatgcttg ttacacctgt cataattagg catggtaaaa ttattgactg aaccctgggg
     7081 gagaaagatg cacagtgatt attataatat ggtttttggt gaaaaattgg ccaatatttt
     7141 atatgaagct aactctcaat ttttttacga gagaaatgtc atagaagagg cagtcaacgc
     7201 tttattctgt gagcgtgaaa taataaacaa caagaacata ataaagaagc tgatgttctt
     7261 tttgtcagat gttaatcaca ccaagaaaga tgttgttcag agcgcactta atattatcat
     7321 tgacattaca tcaggtgata tttgagattt tacataaaag ataatctctc aaggagtctt
     7381 atctgaaaac gaaatatata gtccctctcg ttttgtaata tcgattggct ttgttgaatc
     7441 ggttacgtac cccggagccg gtcatatttt caggtaatac acacgctttc agacatttcg
     7501 gctttcgtca tttttgtatc atgggcatag cttctgctac ctgaccatca taatcacgca
     7561 gcttcagtga agcagtgacc acctgtttaa cccggtgtat cgttatgatc ttatccagc
//
LOCUS       DADLTY010000189         7538 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.190, whole genome shotgun sequence.
ACCESSION   DADLTY010000189 DADLTY010000000
VERSION     DADLTY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.190"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="JN334_003589"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="JN334_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="JN334_003590"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="JN334_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY7439598.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="JN334_003591"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="JN334_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="JN334_003592"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="JN334_003592"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY7439599.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="JN334_003593"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="JN334_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HAY7439600.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /locus_tag="JN334_003594"
     CDS             complement(5060..6487)
                     /locus_tag="JN334_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY7439601.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="JN334_003595"
     CDS             complement(6510..7202)
                     /locus_tag="JN334_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAY7439602.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1916 a         1829 c         1986 g         1807 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgaccatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ccggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgc aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtgt tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccgggat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagaaaaa gcaaaaataa atgattgact ctgtagcggg aaagcgtatt
     7501 atgcacaccc ccccccgcgc cgctgagaaa aagcgaag
//
LOCUS       DADLTY010000190         7448 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.191, whole genome shotgun sequence.
ACCESSION   DADLTY010000190 DADLTY010000000
VERSION     DADLTY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.191"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="JN334_003596"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="JN334_003596"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAY7439603.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="JN334_003597"
     CDS             complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="JN334_003597"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAY7439604.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="JN334_003598"
     CDS             complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="JN334_003598"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706092.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HAY7439605.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /locus_tag="JN334_003599"
     CDS             complement(3024..3479)
                     /locus_tag="JN334_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HAY7439606.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="JN334_003600"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="JN334_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAY7439607.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="JN334_003601"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="JN334_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAY7439608.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="JN334_003602"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="JN334_003602"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HAY7439609.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
BASE COUNT         1701 a         1915 c         2089 g         1743 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aatttgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgggcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtgggg
      481 tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg
      661 cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag
     1681 acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc
     2341 tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc
     2521 agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaaaataacc
     3841 gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag
     4021 ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt
     4381 gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa gtggttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga
     5341 tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca
     5461 atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg
     5581 cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg
     5701 tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc
     5881 gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag
     6001 cagaacgcgc cgcggcgcaa agtgagccgg agatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac
     6661 aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg
     6781 cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc
     7261 cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa
     7321 aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca aacacggcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccgggg
//
LOCUS       DADLTY010000191         7399 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.192, whole genome shotgun sequence.
ACCESSION   DADLTY010000191 DADLTY010000000
VERSION     DADLTY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.192"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..182
                     /locus_tag="JN334_003603"
     CDS             <1..182
                     /locus_tag="JN334_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HAY7439610.1"
                     /translation="LLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTWHDF
                     LPSGTASVSEKHSER"
     gene            172..1886
                     /gene="hyfG"
                     /locus_tag="JN334_003604"
                     /pseudo
     CDS             172..1886
                     /gene="hyfG"
                     /locus_tag="JN334_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            1896..2441
                     /gene="hyfH"
                     /locus_tag="JN334_003605"
     CDS             1896..2441
                     /gene="hyfH"
                     /locus_tag="JN334_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HAY7439611.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            2438..3196
                     /gene="hyfI"
                     /locus_tag="JN334_003606"
     CDS             2438..3196
                     /gene="hyfI"
                     /locus_tag="JN334_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HAY7439612.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            3189..3602
                     /gene="hyfJ"
                     /locus_tag="JN334_003607"
     CDS             3189..3602
                     /gene="hyfJ"
                     /locus_tag="JN334_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HAY7439613.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            3632..5461
                     /locus_tag="JN334_003608"
     CDS             3632..5461
                     /locus_tag="JN334_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAY7439614.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            5462..5644
                     /locus_tag="JN334_003609"
     CDS             5462..5644
                     /locus_tag="JN334_003609"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HAY7439615.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            5666..6514
                     /locus_tag="JN334_003610"
                     /pseudo
     CDS             5666..6514
                     /locus_tag="JN334_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(6552..>7399)
                     /locus_tag="JN334_003611"
     CDS             complement(6552..>7399)
                     /locus_tag="JN334_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7439616.1"
                     /translation="VLVVFVGILLLMAFVVLPIAWQQGIYLIRDMPGMLNKLSDFAAT
                     LPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLLTIAVYLVLVPLMVF
                     FLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVLEMIVVGIATWLGFL
                     LFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGTEFWSCFAVYLIIQA
                     LDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPLATLIKAVIHAWPDG
                     QIAQE"
BASE COUNT         1686 a         1982 c         1973 g         1758 t
ORIGIN      
        1 ttctgctggt catgatgctg gcgatgggaa cgcatattcc acaacctgtc atcaggatcc
       61 tggcgggcgc ttccactata gtcctctcag ggacgcacga cctgcctgca caacgtagca
      121 cctggcatga ttttttgcct tcaggcaccg catctgtttc ggagaaacac agtgaacgtt
      181 aattcatcgt caaatcgtgg cgaagcgatt ctcgccgccc tgaaaacgca gttccccggc
      241 gcggtgctgg atgaagagcg acaaacgcct gaacaggtca ccattacggt gaaaatcaat
      301 ctgctgcctg acgttgtaca ttatctttat tatcaacatg atggctggct tccagtcctg
      361 tttggcaacg acgagcggac acttaacggt cattacgcgg tttattatgc cctttcaatg
      421 gaaggggccg aaaaatgctg gatcgtggtg aaggcactgg tcgatgccga cagtcgggag
      481 tttccgtcag tcacaccgcg cgtccctgcc gcggtctggg gcgagcgaga aattcgcgat
      541 atgtacgggc tgattccggt tggcctgccg gatcagcgtc gcctggtgtt gcccgatgac
      601 tggccggaag atatgcatcc gctgcgcaaa gatgcgatgg attatcgact gcgccctgaa
      661 ccgacgacag attccgaaac gtatgcgttt atcaatgagg gcaacagcga tgcgcgggtg
      721 atccctgtcg gcccgctgca tatcacctcc tatgaaccgg gtcacttccg cttgtttgtg
      781 gatggcgagc aaattgtcga tgctgattac cgcctgtttt atgtccatcg cggcatggag
      841 aaactggcag aaacgcggat gggctacaac gaagtgacct tcttatcgga ccgcgtgtgt
      901 gggatttgcg gttttgccca cagtgtggcc tataccaact cggttgaaaa tgcactgggg
      961 attgaggtgc cgcaacgagc acatactatt cgctcgattc tgctggaagt cgagcggcta
     1021 cacagtcatt tgctcaacct tggcctctcc tgccattttg ttggttttga taccggcttt
     1081 atgcaatttt tccgcgtgcg ggaaaagtcg atgacgatgg cggaattgct gaccgggtcg
     1141 cgtaaaacct acggtctgaa tctgattggt ggtgttcgcc gcgatattct caaagagcaa
     1201 cgtctgcaaa cgctgaaact ggtgcgcgag atgcgcgccg acgtgtcgga gctggtagag
     1261 atgctgcttg ctacgccgaa tatggaacaa cgcactcagg gcattggcat tctcgaccga
     1321 caaatcgccc gtgattatag ccctgtaggg ccgctgatcc gcgcagtggt tttgcccgtg
     1381 atttgcgctt tgatcacccc tacgccgact acggcaatat tccaaaaaca ctgtttacct
     1441 ttaccggcgg cgatgttttc tcccgcgtga tggtccgtgt caaagagacg tttgattcgc
     1501 tggcaatgct ggaatttgcc ctcgacaaca tgccggatac cccactgctg accgaaggct
     1561 ttagctataa acctcacgca ttcgcgctgg gctttgttga agcgccacgc ggtgaagacg
     1621 tgcactggag catgctcggt gataaccaaa aattgttccg ctggcgctgt cgtgccgcca
     1681 cctacgccaa ctggccggtg ttgcgttaca tgctgcgcgg caataccgtt tctgacgcac
     1741 cgctgattat cggtagcctt gatccctgct actcctgtac cgaccgtgtg acgctggtag
     1801 atgtgcgcaa gcgccagtca aaaaccgtgc tgtataaaga gatcgaacgc tacggcattg
     1861 atcgtaaccg ttcgccgctg aagtaaggac agaagatgct gaagttactg aaaactatta
     1921 tgcgcgccgg aaccgcgacg gtgaaatatc ccttcgcgcc actggaggtc agcccaggct
     1981 ttcgcgggaa accggacctg atgcccagcc aatgtattgc ctgcggtgcc tgcgcctgtg
     2041 cttgtccggc aaatgcgctg actatccaga ccgacgacca gcaaaattcg cgcacctggc
     2101 agctctatct ggggcgttgt atttactgcg gacgttgtga agaagtgtgc ccgaccagag
     2161 ccatccagct taccaataac tttgaactga ccgtcaccca taaagccgat ctctataccc
     2221 acgcgacgtt ccatctacaa cgttgcagcc gttgcgaacg cccgtttgcc ccgcaaaaaa
     2281 ccgtcgcact ggctgctgaa ttgttggcac agcaacaaaa tgctccacaa aaccgcgaaa
     2341 tgttatgggc gcaagcgagc gtctgcccgg agtgcaaaca acgcgcaacg ctgatcaacg
     2401 acgatacaga tgtaccgctg gtggctaagg agcagctatg agtccagtgc ttacgcaata
     2461 tgtcagccag cccatcacgc tggacgagca aacgcaaaag atgaagcggc atttgctaca
     2521 ggatatccgt cgctcggctt acgtttatcg catcgattgc ggcggctgca acgcctgtga
     2581 aatcgaaatt tttgctgcca tcacccccgt attcgacgca gaacgttttg gcattaaggt
     2641 tgtttcatca ccgcgtcacg ccgatatttt gttatttact ggcgcagtca cccgggcgat
     2701 gcgtatgcct gcacttcggg cgtatgagtc tgcccccgat cataaaatct gtgtttccta
     2761 cggcgcgtgc ggtgtcggcg gcggtatttt ccacgatctc tacagcgtct ggggcggcag
     2821 cgacaccatt gtccccattg atgtttggat ccccggctgc ccgccaacac cggccgccac
     2881 cattcacggt ttcgccgtgg cgctcggttt gctgcaacag aagattcacg cggtggatta
     2941 tcgcgatccc accggggtga ctatgcaacc gttgtggccg cagatcccgc catcacagcg
     3001 tatcgccatt gagcgagaag cgcggcggct ggcgggctat cgtcaggggc gagaaatttg
     3061 cgatcggctc ctgcgccatt taagcgacga tcctacagga aatcgggtta acacctggtt
     3121 gcgcgatgcc gacgatccac gtctcaatag tatcgttcag caactctttc gcgtactccg
     3181 ggggttacat gactgaagag tgcggggaaa ttgttttctg gacgctgcga aaaaagtttg
     3241 tcgccagtag cgacgagatg ccggaacaca gctctcaggt aatgtattac tcgctggcta
     3301 tcggtcatca cgttggcgtg attgattgtc tgaatgtcgc cttccgctgc ccactgacgg
     3361 aatacgaaga ttggcttgca ctggtcgaag aggagcaagc ccgacgtaag atgctggggg
     3421 tgatgacttt tggtgagatt gttattgacg ccagccacac cgccctgttg acccgggcat
     3481 tcgcgccact ggcagatgac gcgacgtctg tgtggcaagc gcgtagcatt caattcattc
     3541 atctgttgga tgaaattgtg ctggaaccgg ctatctatct gatggccaga aaaattgcgt
     3601 gagaaggatt tctcattaat aaggactgtt gatggctatg tcagacgaag cgatgtttgc
     3661 cccgccgcaa ggaataacaa ttgaagcggt aaacggaatg ctcgcggagc ggttagcgca
     3721 gaaacacggc aaggcgtctt tattacgcgc cttcatcccg ctgccgccgc cgttcagccc
     3781 ggtacaactt attgaactgc atgttctcaa aagcaacttc tattaccgct accatgatga
     3841 tggcagcgat gtgacggcaa caacagagta tcagggcgag atggtcgatt attcgcgtta
     3901 cgccgtcctt ctcggcagta gtggaatggc ggagctacgc tttattcgta cccacggcag
     3961 ccgttttact ccccaggatt gcacactgtt taactggctg gcacgcatta tcaccccggt
     4021 tctgcaatca tggctcaatg atgaagcaca gcaggtggcg ctgcgtttgc tggagaaaga
     4081 tcgcgatcat catcgggtac tggtggatat cactaatgca gtgctgtcac atcttgatct
     4141 cgacgatctg atcgctgacg tcgctcgtga gatccatcat tttttcggtc tggcttcagt
     4201 cagtatggta ctgggcgatc atcgaaagaa cgagaagttc agcctgtggt gcagcgatct
     4261 ttctgcctca cattgtgcgt gtctgccaca caatatgcct ggcgacagtg tattgctgac
     4321 acaaacgcta caaacccgac aaccgacctt gacgcaccgt gcagatgatc tgtttctctg
     4381 gcaacgcgac ccgttattac tcttacttgc atctaacggc tgcgaatctg cgctccttat
     4441 accgcttacc tttggcaacc atacaccggg tgcattgttg ctggcgcata cctcttccac
     4501 tctctttagt gaggaaaact gccagctact acaacacata gccgatcgca tcgctattgc
     4561 cgttggcaat gccgatgcct gccgtagaat gaccgatttg caggaaagtt tgcagcaaga
     4621 aaaccaccag cttagcgagc agctcctttc gaatctgggc gtcggtgaca ttatctatca
     4681 aagccaggca atggaagacc tgctccagca ggtagatatt gtggcgaaga gcgacagtac
     4741 ggtgttgatt tgcggtgaaa ccggaaccgg caaagaggtg atcgccagag cgatccatca
     4801 acttagcccg cgacacgaca agccgctggt caaaatcaac tgcgctgcca tccccgccag
     4861 tcttctggaa agtgagttat tcggtcatga caaaggggcg tttactggtg cgattaatac
     4921 ccatcgtggt cgttttgaaa ttgccgatgg cggcacgttg tttctcgatg aaattggcga
     4981 tctgccgtta gaacttcagc ctaaactgct gcgcgtattg caggaacggg agattgagcg
     5041 tctcggcggg agtagaacga tcccggtaaa tgtcagagtc attgccgcca ccaaccgtga
     5101 tttgtggcaa atggttgaag atcgccagtt tcgcagcgat ctcttttatc gcctgaatgt
     5161 cttcccactg gaattgcctc cgctgcgcga ccgtccggaa gatatccctc ttttagcaaa
     5221 gcatttcacg caaaaaatgg cgcgccatat gaatcgttca attgacgcca tcccgaccga
     5281 ggcgctacgc cagttgatgt cgtgggactg gccgggcaac gtgcgcgagc tggaaaacgt
     5341 gattgagcga gcggtactgc tgactcgcgg taacagtctg aatttacatc ttaatgtccg
     5401 acaaagccgt ttactgccga cgctaaatga agattcagcg cttcgcagtt caatggcgta
     5461 gttgctgcac ccgacgacgc cagagaatga cgaagaagaa cgtcagcgca ttgttcaggt
     5521 attgcgagaa accaatggca ttgttgccgg gccccgtggc gcggcgacac gattagggat
     5581 gaagcgcacc acgctgctgt cacgaatgca gcgtctggga atctcggttc gcgaggtgtt
     5641 gtaatctgct tttgcaggag tatgcatgag aaacaaactc tctttcgact tgcagttgag
     5701 cgccagaaaa gcggcaatcg ctgaacggat tgccgcccat aaaattgccc gcagtaaagt
     5761 gtcggtcttt ttaatggcga tgtccgctgg cgtgtttatg gcgatcggat ttacttttta
     5821 cctttccgtt atcgccgatg ccccgtcttc ataggcatta acccatctgg tgggcggcct
     5881 ttgctttaca cttggcttta ttttgctggc ggtttgcggc accagcctgt tcacctcgtc
     5941 ggtaatgacg gtaatggcaa aaagtcgggg cgttattagt tggcgaactt ggctgattaa
     6001 cgcacttctg gtggcctgcg gtaatctggc aggtattgcc tgtttcagtt tgttaatctg
     6061 gttttccggg ctggtgatga gtgaaaacgc gatgtgggga gtcgcgattt tacactgcgc
     6121 cgagggcaaa atgcatcata catttactga atctgtcagc ctcggcatta tgtgcaatct
     6181 gatggtttgc ctggcgctgt ggatgagtta ttgcggacgt tcgttatgcg acaaaatcgt
     6241 cgccatgatt ttgtccatca ccctgtttgt cgccagtggc tttgagcact gtatcgccaa
     6301 tttgtttgtg attccgttcg ccattgccat tcgccatttc gcccctacct ccttctggca
     6361 actggcgcac agtagcgcag acaattttcc ggcactgacg gtcagccatt ttattaccgc
     6421 caatctgctc ccggtgatgc tgggtaatat tatcggcggt gcagtgctgg tgagtatttg
     6481 ttatcgggct atttatttac gtcaggaatc ctgaaaaatc agcccggcga aacagttcgt
     6541 cgggctgaag attattcttg cgcgatttgc ccatcgggcc aggcgtggat cacggctttg
     6601 atcagcgtcg ccaatggaat ggcgaagaat acgccccaga atccccacaa accaccgaag
     6661 atcaccaccg ataaaataat caccagcgga tgcaggttaa ccgcttcgga gaacaacacc
     6721 ggtactagca ggttgccgtc cagcgcctga ataatcagat acaccgcgaa gcagctccag
     6781 aattcagtac ctgcgccaaa ctggaatagc gccacgccaa ccaccggaat ggtcaccaca
     6841 aatgcgccaa tgtacggaat aagaaccgag aagccgacca gcactgccag cagcagcgaa
     6901 tagttcagcc cgaagagcaa gaaccccagc caggtggcga tccccaccac gatcatctcc
     6961 agcactttgc cgcggatata gttggtgatt tgttgattca tctccttcca cacctgtcct
     7021 gccagcccac ggttacgcgg cagcacccgg cgaacggcgt tcagcatctg ctctttgtct
     7081 ttcagcagga agaagaccat caatggcacc agcaccagat agacggctat ggtcagcaaa
     7141 ccgaccagcg aggcgaggga aattttcacc accgaatcgc ccatggtcag catccgactg
     7201 cgcatatttt cggccattgc atcaataatg cccgcgtcca ttaacaccgg atagcggcgc
     7261 ggcaacgtgg cggcaaagtc agaaagctta ttgagcatcc ccggcatatc gcggattaag
     7321 tagatgccct gttgccaggc gataggcagt accacgaacg ccatcagtag caatataccg
     7381 acaaaaacca ccaatacaa
//
LOCUS       DADLTY010000192         7318 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.193, whole genome shotgun sequence.
ACCESSION   DADLTY010000192 DADLTY010000000
VERSION     DADLTY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.193"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..898
                     /locus_tag="JN334_003612"
     CDS             <1..898
                     /locus_tag="JN334_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439617.1"
                     /translation="ALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTGHRD
                     IEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVARLL
                     GPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAICCV
                     IGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDRLPS
                     RSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGSAL
                     LFRRMALVELAVLVVIIVSRGA"
     gene            901..1824
                     /gene="hycD"
                     /locus_tag="JN334_003613"
     CDS             901..1824
                     /gene="hycD"
                     /locus_tag="JN334_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HAY7439618.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            1842..3551
                     /locus_tag="JN334_003614"
     CDS             1842..3551
                     /locus_tag="JN334_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HAY7439619.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            3561..3680
                     /locus_tag="JN334_003615"
     CDS             3561..3680
                     /locus_tag="JN334_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HAY7439620.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            3691..4704
                     /locus_tag="JN334_003616"
     CDS             3691..4704
                     /locus_tag="JN334_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7439621.1"
                     /translation="MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEES
                     SYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKH
                     SAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSV
                     WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLI
                     ANGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGF
                     DDIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIA
                     LSR"
     gene            4853..5380
                     /gene="hydN"
                     /locus_tag="JN334_003617"
     CDS             4853..5380
                     /gene="hydN"
                     /locus_tag="JN334_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HAY7439622.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            5533..>7318
                     /gene="hypF"
                     /locus_tag="JN334_003618"
     CDS             5533..>7318
                     /gene="hypF"
                     /locus_tag="JN334_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAY7439623.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCG"
BASE COUNT         1527 a         1917 c         2159 g         1715 t
ORIGIN      
        1 cgcgctcgaa caaccggcgc tgattgctct tggcctggtc ggtggtctgt accatctgct
       61 taaccatagc ctgttcaaaa gcgtactgtt cctcggggcg gggagcgtct ggttccgtac
      121 cggtcatcgc gatatcgaaa aactcggtgg tattggcaag aaaatgccgg ttatctccat
      181 cgccatgtta gtcgggttga tggcaatggc tgcgctgccg ccactgaacg ggtttgccgg
      241 ggaatgggtt atctatcaat ccttcttcaa actgagcaat agcggcgcgt ttgttgcccg
      301 tctgctgggg ccgctgctcg ctgtgggtct ggcaattacc ggtgcgctgg cggtgatgtg
      361 tatggcgaaa gtctatggcg tcacgttcct cggcgcgccg cgtaccaaag aagccgaaaa
      421 cgccacctgt gcgccgctcc tgatgagcgt aagcgtagtg gcactggcga tttgctgcgt
      481 aattggcggt gttgctgcgc cgtggctgct gccgatgctc tctgctgctg tacctctgcc
      541 tctggagcct gctaacacca ccgtttctca accaatgatc acattgctgc tgattgcctg
      601 cccgctgctg ccattcatca ttatggcgat ttgcaaaggc gaccgcctgc cgtcacgttc
      661 ccgcggtgcg gcctgggtgt gcggctacga ccatgaaaaa tcaatggtga ttaccgctca
      721 cggttttgcc atgccggtta aacaggcgtt tgcgccagtg ctgaaactgc gcaaatggct
      781 gaatccggtg tcgctagtgc cgggctggca gtgcgaaggt agtgcgttgc tgttccgccg
      841 gatggcgctg gttgaactgg cggtactggt ggtgattatt gtttcacgag gagcctgaga
      901 atgagtgttt tatatccgtt aattcaggcg ctggtgttat ttgccgttgc gccgctgctc
      961 tccggtatta cccgcgtggc gcgcgcccgt ctgcataacc gtcgcgggcc gggcgtgttg
     1021 caggagtatc gcgacattat caaactgctg ggccgccaga gcgttggccc ggatgcctct
     1081 ggctggatgt tccgcctgac gccgtatgtg atggtgggcg tcatgctgac tatcgctact
     1141 gcgctgccgg tggtgaccgt cggttctccg ctgccgcaac tgggcgattt gatcacctta
     1201 ctgtatctct tcgccatcgc gcgtttcttc tttgccattt ccggtctgga taccggtagc
     1261 ccgtttaccg ctatcggtgc gagccgtgaa gcgatgcttg gcgtgctggt cgaaccgatg
     1321 ttgctgcttg gtctgtgggt tgccgcacag gttgccggtt ccaccaacat cagcaacatt
     1381 accgacaccg tttatcactg gccgctgagc caaagcatcc cgctggtgct ggcgctctgc
     1441 gcctgtgcgt tcgccacttt tatcgaaatg ggcaaactgc cgttcgacct ggcggaagcc
     1501 gagcaggagt tgcaggaagg tccgctctcc gaatacagcg gtagcggctt tggcgtcatg
     1561 aaatggggta tcagcctgaa acagctggtg gtgttgcaga tgttcgtcgg ggtgtttatt
     1621 ccgtggggac aaatggaaac cttcaccgtc ggtggactgc tgctggcgct ggtgattgcc
     1681 atcgtcaaac tggtggtcgg cgtcctggtt atcgcgctgt tcgaaaacag catggcccgt
     1741 ctgcgtcttg atattactcc gcgcattacc tgggctgggt ttggctttgc atttttagcg
     1801 ttcgtctcct tgctggcggc gtgattaaag agagtttgag catgtctgaa gaaaaattag
     1861 gtcaacatta tctcgccgcg ctgaatgagg catttctagg cgtcgtgctg gaccacgcct
     1921 ggcagaccaa agatcagctg actgtcaccg taaaggtgaa ctatctgccg gaagtggtgg
     1981 agtttctcta ctacaagcag ggtggctggc tgtcggtgct gtttggtaac gacgaacgca
     2041 aactgaatgg tcattacgcc gtttattacg tgctgtcgat ggagaagggc accaagtgtt
     2101 gggtaacggt tcgcgtcgaa gttgacgcca acaaaccgga gtatccgtcc gtgacgccgc
     2161 gcgttccggc ggcggtgtgg ggcgagcgcg aagtgcgcga tatgtacggt ttgattccgg
     2221 ttggtctgcc ggatgaacgt cgtctggtgc tgccggatga ctggccggat gaactttatc
     2281 cgctgcgtaa agacagcatg gattatcgtc agcgtccggc accgactacc gatgctgaaa
     2341 cctacgagtt catcaacgaa ctgggcgaca agaaaaacaa cgtcgtgccg attggtccgc
     2401 tgcacgtcac ttctgacgaa ccgggtcact tccgtctgtt cgtcgatggc gaaaacatta
     2461 tcgacgccga ctaccgtctg ttctacgtcc atcgcggtat ggaaaaactg gcggaaaccc
     2521 gcatgggtta taacgaagtg accttcctct ctgaccgtgt gtgcgggatc tgcggctttg
     2581 cccacagcac cgcctacacc acgtcggtgg aaaacgcgat gggtattcag gtgccagaac
     2641 gcgcgcagat gatccgcgcc attctgctgg aggtggaacg cctgcacccg catctgctca
     2701 acctcggcct cgcctgtcac tttaccggct tcgactccgg ctttatgcag ttcttccgcg
     2761 tgcgtgaaac ctccatgaaa atggcagaga tccttaccgg tgcgcgtaaa acctacggcc
     2821 tgaacctgat cggcgggatt cgtcgcgatc tgctgaaaga tgacatgatc cagacccgcc
     2881 agctggcgca acaaatgcgt cgtgaagtgc aggagctggt ggatgtgctg ctgagtacgc
     2941 cgaacatgga acaacgcacc gtcggcattg gtcgtctgga cccggaaatc gcccgcgatt
     3001 tcagtaacgt tggcccgatg gttcgcgcca gcggtctcgc ccgcgatact cgcgccgatc
     3061 acccgtttgt cggttatggc ctgctgccaa tggaagtcca cagcgagcag ggctgcgacg
     3121 ttatttcccg tctgaaagtg cgaatcaacg aggtctatac cgcgctgaat atgatcgact
     3181 acggtctgga taacctgccg ggcggtccgc tgatggtgga aggctttacc tacattccgc
     3241 accgtttcgc gctgggcttt gccgaagcgc cgcgcggcga cgatatccac tggagcatga
     3301 ccggcgacaa ccagaagctg taccgctggc gctgccgtgc cgcgacctac gcgaactggc
     3361 cgaccctgcg ctacatgctg cgtggcaata ccgtttccta tgcgccgctg attatcggta
     3421 gcctggaccc ttgctactcc tgtaccgacc gcatgaccgt agtcgatgtg cgtaagaaga
     3481 agagcaaagt ggtgccgtac aaagaactcg agcgttacag cattgagcgt aaaaactcgc
     3541 cgctgaaata aggaatcgcc atgtttacct ttatcaaaaa agtcatcaaa accggcacgg
     3601 cgacctcgtc ttatccgctg gagccgattg cgcaaggaca atcggcaaac ggtgcagtac
     3661 actgcgttga ttgcgtgtaa agggatgaaa atgatgacga cgatgctgga agtggcgaag
     3721 cgcgccgggg tttcaaaagc gaccgtttcc cgcgtgcttt caggtaatgg ctacgtcagc
     3781 caggagacta aagatcgcgt gtttcaggcg gtagaagaga gcagttaccg cccaaatttg
     3841 ctggcccgca atctgtcggc gaagagtact cagacgctgg ggctggtagt gaccaacacg
     3901 ctttatcacg gcatttattt tagtgaatta ctgtttcatg ccgcgcgaat ggcggaagag
     3961 aaagggcgac agttgctatt ggcagatggt aaacacagcg ccgaagaaga gcgccaggcg
     4021 attcagtatc tgctggattt gcgctgcgac gccattatga tttacccgcg ttttttaagc
     4081 gtggatgaga ttgatgacat cattgacgcg cacagtcagc cgataatggt gcttaatcgc
     4141 cgcctgcgca ataatagcag ccatagcgtc tggtgcgatc ataaacagac cagctttaac
     4201 gccgtggcag agttgataaa cgccgggcat caggagattg ctttccttac cggctcgatg
     4261 gattccccca ccagcattga acgtcttgcc gggtataaag acgcgctgtc gcagcatggt
     4321 attgcgctca atgaaaaact tatcgctaac ggtaaatgga cgcccgccag cggggccgaa
     4381 ggggtagaaa cgttgctcga acgaggggct aaatttagcg cgttagttgc cagtaacgac
     4441 gatatggcga taggtgcgat caaagcgttg cacgagcgcg gcgtagcggt gccagagcag
     4501 gtgtcagtta tcggattcga tgatatcgca attgccccct atatcgttcc ggcgctctcc
     4561 agcgtaaaaa ttccggtgac tgagatgatt caggaaatta ttggacggct gatttttatg
     4621 ctcgatggcg gggatttctc accgccgaaa acctttagcg gaaaactgat ccgccgcgac
     4681 tcactcattg ccctttcgcg ataaacattg tcatcacatg tcatcgtcac tagtgtcgat
     4741 gacatctttt ttcctgcatt acagaaatta atttccttaa ataacagcaa attaaaaact
     4801 ggcatgattt gtgagtgtat cggcgcatta actgtcattg ctggagaatt tgatgaaccg
     4861 tttcatcatt gctgacgcga gtaaatgtat tggttgccgt acctgtgaag tggcgtgcgt
     4921 ggtttctcat caggaaaatc aggactgtgc atcgctgacc ccggaaacat ttttaccgcg
     4981 tatccatgtc attaaaggtg tgaacatttc cacggcgaca gtttgccgtc agtgtgaaga
     5041 tgcaccgtgc gctaacgtct gcccgaatgg tgctatcagc cgtgataaag ggtttgttcc
     5101 tgtgatgcag gaacgttgca ttggttgcaa aacctgcgtg gtggcttgcc cgtatggcgc
     5161 gatggaagtg gtggtacgtc ctgtgattcg caacagcggc gcgggtctga atgtacgggc
     5221 tgacaaagcc gaagccaata aatgcgacct gtgcaaccat cgtgaagacg gcccggcgtg
     5281 tatggcggct tgcccgaccc atgcgctgat ttgtgtcgat cgtaataaac ttgaacaact
     5341 gagcgcagaa aaacgccgcc gtacggcgct gatgttctaa aaatcttaca gaactgttct
     5401 ggcatcaaag gaattcccgg aaatattcgt agaatacgga gcgggcggta tcgccgacct
     5461 gagactgccg gatacgacgc tgtcgtgtcc ggcagggcgg gttacgcaat tcatgatggc
     5521 gggaataact caatggcaaa aaacacatct tgcggtgtcc aactgcgtat tcgtggcaaa
     5581 gtgcagggcg tcggttttcg tccgtttgtc tggcagctgg cacagcaatt aaatcttcac
     5641 ggcgatgtat gtaatgacgg cgatggcgta gaagtccggc tgctggaaga cccggaaacg
     5701 tttcttgttc aattgcatca gcactgtccg ccactggcgc gtattgatag cgtcgagcgt
     5761 gagccgttta tctggtcaca actgcccacc gagttcacca tccgccagag caccggcggc
     5821 accatgaata cgcaaattgt cccggatgcc gccacttgcc ctgcttgcct tgccgaaatg
     5881 aataccccag gcgagcggcg ttatcgttat ccgtttatca actgtaccca ctgcggcccg
     5941 cgtttcacca ttattcgcgc gatgccttac gaccgcccgt ttaccgtgat ggcggcgttt
     6001 ccgctatgtc cggcctgtga caaagagtac cgcgacccgc tcgatcgtcg cttccacgcc
     6061 cagccggtgg cctgcccgga gtgtggcccg catcttgaat gggtaagtca tggtgaacat
     6121 gcagaacaag aggcggcatt acaggcagct atcgcacagt taaaaatggg caacattgtc
     6181 gccatcaaag ggattggcgg atttcatctt gcctgcgatg cacgtaacag taacgcggtg
     6241 gcgacactgc gggagcgcaa acatcgcccg gcgaaaccgc tggcagtcat gttgccagtg
     6301 gcagaaagtt taccagacgc tgcgcgccag ttgcttacca cgcccgctgc gccgattgtg
     6361 ctggtggata aaaaatacgt acctgagctt tgtgatgata tcgcccctgg ccttaacgaa
     6421 gtcggggtga tgttgcctgc gaacccgctc cagcatttgc tgttacagga actgcaatgc
     6481 ccgctggtga tgacctccgg caacctgagc ggtaaaccac cagctatcag caacgaacag
     6541 gcgctggcgg atttgcaggg cattgccgac ggattcttgc tacataaccg cgacatcgtg
     6601 cagcggatgg atgattcagt ggtgcgcgaa agcggcgaaa tgctgcgccg ttcgcggggg
     6661 tatgtgccgg atgcgctggt tttgcctccg ggctttaaaa atgttccgcc tgtgctgtgt
     6721 cttggcgcgg atctgaaaaa taccttctgc ctggtgcgcg gtgaacaagc ggtgttgagt
     6781 cagcatctgg gcgatttaag tgacgatggc atccagatgc agtggcgcga agcgttacgc
     6841 ctgatgcaaa acatctacga tttcactccg caatacattg tgcatgatgt gcatccgggc
     6901 tatgtctcca gccagtgggc gagcgaaatg aatctgccga cgcaaacggt actgcatcat
     6961 catgcccatg cagcggcgtg tctggcagag catcagtggc cgctggatgg cggtgatgtc
     7021 attgctttga cgctcgacgg tatcggtatg ggggagaatg gcgctttgtg gggcggcgag
     7081 tgcctgcggg tgaactatcg cgaatgtgag cacctgggcg gcttgcctgc agtggcgctt
     7141 ccgggtggcg atttggcggc gaagcagccg tggcgaaacc tgctggcgca gtgcctgcgc
     7201 tttgtgccgg agtggcagaa ttaccctgaa acggcaagtg tgcaacagca aaactggagc
     7261 gtactggcgc gggccattga gcgtggaatt aacgcgccgc tggcatcatc gtgtgggc
//
LOCUS       DADLTY010000193         7307 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.194, whole genome shotgun sequence.
ACCESSION   DADLTY010000193 DADLTY010000000
VERSION     DADLTY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.194"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..698)
                     /gene="fadA"
                     /locus_tag="JN334_003619"
     CDS             complement(<1..698)
                     /gene="fadA"
                     /locus_tag="JN334_003619"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="FadA; fatty acid oxidation complex component beta;
                     functions in a heterotetramer with FadB; similar to
                     FadI2J2 complex; functions in beta-oxidation of fatty
                     acids; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HAY7439624.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTV"
     gene            complement(708..2897)
                     /gene="fadB"
                     /locus_tag="JN334_003620"
     CDS             complement(708..2897)
                     /gene="fadB"
                     /locus_tag="JN334_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HAY7439625.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            3087..4418
                     /gene="pepQ"
                     /locus_tag="JN334_003621"
     CDS             3087..4418
                     /gene="pepQ"
                     /locus_tag="JN334_003621"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAY7439626.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4418..5032
                     /locus_tag="JN334_003622"
     CDS             4418..5032
                     /locus_tag="JN334_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HAY7439627.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            5071..6522
                     /gene="trkH"
                     /locus_tag="JN334_003623"
     CDS             5071..6522
                     /gene="trkH"
                     /locus_tag="JN334_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HAY7439628.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            6534..7079
                     /gene="hemG"
                     /locus_tag="JN334_003624"
     CDS             6534..7079
                     /gene="hemG"
                     /locus_tag="JN334_003624"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HAY7439629.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         1624 a         1941 c         1994 g         1748 t
ORIGIN      
        1 accgtaccgt ttactggatc aaacgccgga cgcagcgtgg cgagggcttc cacggtggtt
       61 tccgggcgaa tcacttcgtc gtaattaaac tgcttcagga cgccgtcggc atcgtgacca
      121 ccagttggga tgatttcatt tttaaatgcg gccgactgcg tggcggccca ggcgcgggcg
      181 tgtgaccgcg cggcaaaggc atcctgcatt tcacggctga taccgtgcat acgcgccagc
      241 atttctgccg ttaagcccat catgcccgcc gctttggcga cattgcggct caggccgggg
      301 tgaaaatcga cgccgtgact catcggcaca tggcctatat gctccacgcc gccaaccaga
      361 catgcctgcg tatcgccagt catgatcatt cgtgctgcgt catgcagtgc ctgcatggat
      421 gaaccacaca agcgattgac ggtaaccgcc gggacagagt gcggcacttc tgccagcagc
      481 gccgcgttac gggcgatgtt aaaaccctgc tccagcgtct gttgcacaca accccagtaa
      541 atatcgtcga gggccgccgc ttccagcgcc gggttacgcg ccagcaggct acgcattaaa
      601 tgagcggaga gatcttccgc acgcacgtta cgaaaagcac cgcccttcga acggcccatc
      661 ggggtgcgaa ttgcatcgac aatgacaacc tgttccattg tgactcctta agccgttttc
      721 aggtcgccaa ccggacgggc tggctcaacc ggaggatagt acggttcgtt atgacgcgct
      781 ttattacgca gaccttccgg cacttcatac agcgggccga ggtgctgata ttgctgtgcc
      841 atatcgaggt attttgcgct accgagggtg tccagccagc ggaacgcgcc gccgtggaac
      901 ggagggaagc ccaggccgta gaccagcgcc atatccgctt ccgccggagt ggcgataatg
      961 ccctcttcca gacagcgcac cacttcgttg accatcggga tcatcatgcg ggcgataatc
     1021 tcttcttcgc tgaaatcgcg cttcggttgg ctcacttctg ccagcaggtc ttcaacggcg
     1081 gcgtcttctt ctttcttcgg cttacctttg ctgtcttctt tataacgcca gaaaccgagg
     1141 ccgttcttct gaccaaagcg gttggcatca aacagcgcgt cgatggcatc gcggtaatct
     1201 ttctgcatcc gctgcgggaa gcctgctgcc atgacagcct gagcgtgatg cgcggtatca
     1261 atgcccacaa cgtccagcag atatgccggg cccatcggcc agccaaactg tttttccatc
     1321 actttgtcga tcttgcggaa atccgcgccg tcgcgcagca gctggctgaa accggcgaaa
     1381 tacgggaaca gcacgcggtt aacaaagaag ccggggcagt cgttaaccac aatcggcgtc
     1441 ttgcccatct tgctcgccca ggcgacaact ttcgcgatgg tttcgtcgga gcttttctcg
     1501 ccgcgaataa tttctaccaa cggcattcgg tggaccgggt taaagaagtg catcccgcag
     1561 aagttttccg ggcgttccag cgcgttggcc agttcgctga taggaatggt tgaagtgtta
     1621 gacgccagca cggtatcctg gcgtactttt tgttcggttt ctgccagtac ggcttttttc
     1681 actttcgggt tttcaacaac cgcttctacc acaatatcca cgcggtcaaa tccggcgtag
     1741 tcgagcgttg ggtggatcgt ggagatcacg ccagccagtt tcagaccatc gattttgccg
     1801 cgctcaagct gcttgttcag cagtttcgcg gcttcggtca tgccgagggt taacgacttg
     1861 tcgttgatat ctttcatgac aaccggcacg cctttccacg cggactggta agcgataccg
     1921 ccacccatga tgcctgcgcc cagcacagca gcctgtttcg gggtttcaac gtctttggtg
     1981 agtttcttcg ctttgccttt tacatattga tcgttaagga aaatgccgac cagtgcgcga
     2041 gcttcgttgg tatgcgccag cgggacaaaa cttttgtttt ccagatttaa ggcttcttca
     2101 cgaccaaaac gggccgcagc ttcaatggtt tttactgcgg tgatgggggc cggataatgt
     2161 ttccccgccg tttgcgcgac cattccttta gcgatggtga agctcatggt ggcttcaatc
     2221 ttgctcagtt ttagcggttc ctgcttcggc tgacgtttcg ctttccagtc gaggtcgccg
     2281 ttaatggcct ggcgtaaaat cgccatcgcc ccttcaatca gtttttctgc tttgactacg
     2341 ccatccacca gaccgatttt cagcgcctga tccgcgccga catctttacc ggcggcaatg
     2401 atttccagtg cactgtcagc gcccagcata cgtggcatac gtacagaacc gccaaagccc
     2461 ggcatgatgc ccagtttggt ttccggcaga ccgatgcgca gatccggcgt cgccagacga
     2521 taatcggtcg ccagcacgca ttcgcagcca ccacccagcg catagccgtt gacggcagca
     2581 atggtcggca ccggcagatc ttccaggcga ttaaacacgc tattggcaaa gtgcagccac
     2641 tgacttaact gttcttcagg aacgaggaac agggacaaaa attcggtgat atcagcaccg
     2701 acgataaagg ctgctttgtt cgaacgcagc agcagccctt ttagatctga ttgctgttcc
     2761 agcacgccga tggcctcgcc gaggctggcg acggtcgcag tgtcgagttt attaactgaa
     2821 cctggggcat caaataccag ttcggcaatg ccatcttcca gccagtcaag gtacagggtg
     2881 tcgcctttgt aaagcatgtc agtctcctga atccgcaagg taatctggtc gtaccagatg
     2941 catcggagtg tgtgttttgt gttaaaaata tgcaaataaa agattaaaga aatgccgatc
     3001 tgatcacgct cggcagaaat cacgctctgg atgaacgatg tgctaagatg cggagactta
     3061 aggtcaaaaa aacagaaggg taaaaaatgg aatcactggc ctcgctctat aaaaatcata
     3121 tagctacctt acaagaacgg actcgcgatg cgctggcgcg cttcaagctg gatgcattac
     3181 ttattcactc cggcgagctg ttcaacgttt ttctcgacga tcatccctat ccgtttaaag
     3241 tgaacccgca attcaaagcg tgggtgccgg taactcaggt gccaaactgc tggttgctgg
     3301 tggatggcgt gaacaagccg aaactgtggt tctatctgcc ggttgattac tggcacaacg
     3361 tcgaaccgct gccgacctcc ttctggactg aagatgtgga agtgatcgcg ctgccgaaag
     3421 ccgatggcat tggtagcctg ctgcctgctg cgcgcggcaa tatcggttat atcggtccgg
     3481 tgccggaacg tgcgctgcaa ctgggtattg aggccagcaa catcaacccg aaaggggtga
     3541 tcgactacct gcattattac cgctccttca aaaccgagta cgaactggcc tgtatgcgtg
     3601 aagcgcagaa aatggcggtc aatggtcatc gtgcggcaga agaagcgttc cgttctggca
     3661 tgagcgagtt cgatatcaat attgcctatc tgaccgcgac cggtcatcgt gataccgacg
     3721 taccttacag caacattgtg gcgcttaacg aacacgctgc ggtgttgcat tacaccaaac
     3781 tggaccacca ggcaccggaa gagatgcgca gcttcctgct ggatgccggg gccgaatata
     3841 acggctatgc ggctgacctg acccgtacct ggtcggcaaa aagcgacaac gactacgcac
     3901 agctggtgaa agacgtaaat gatgaacaac tggcgctgat cgccaccatg aaagcaggcg
     3961 tcagctatgt ggattaccac atccagttcc atcagcgcat cgccaaactg ctgcgtaaac
     4021 atcaaatcat caccgatatg agtgaagaag cgatggtcga aaacgatctc accggaccgt
     4081 ttatgccgca cggtattggc catccgctgg gcctgcaggt gcatgacgtc gccggtttta
     4141 tgcaggatga tagcggtaca cacctcgcgg caccggcaaa atatccgtac ctgcgctaca
     4201 cccgtattct ccagccgggc atggtgttaa ccatcgaacc gggtatctac ttcattgaat
     4261 cgcttctggc accgtggcgt gaagggcagt tcagcaagca cttcaactgg cagaaaattg
     4321 aagcactgaa accgttcggc ggcattcgta tcgaagacaa cgtggtgatc cacgaaaaca
     4381 acgtggaaaa catgacccgg gatctgaaac tggcgtgatg gaaagctggt taattcctgc
     4441 ggcaccggtc acggtcgttg aagagatcaa aaagagccgc ttcataacga tgttggcgca
     4501 taccgatggc gttgaggcgg cgaaagcgtt tgttgaatcg gtgcgggcag aacaccccga
     4561 tgcccgccac cattgcgtgg cgtgggtggc gggtgcgccg gatgattctc aacagctggg
     4621 tttctctgac gacggggagc cggcgggaac ggcaggtaaa ccgatgctcg cccagctaat
     4681 gggcagcggc gtcggggaaa ttaccgctgt ggtagtgcgc tactacggcg gcatattgct
     4741 tggcaccggt gggttagtga aagcgtatgg cggcggcgtg aatcaggcgc tgcgccagct
     4801 aacgacccaa cgcaagacgc cattaaccga atatactttg caatgtgaat atagtcagtt
     4861 aaccggcatt gaagcgttgc tggggcagtg tgacggcaaa attatcaaca gtgattatca
     4921 ggcattcgtt ctgctgcggg tggcgcttcc ggcggcgaaa gtggctgaat tttccgcaaa
     4981 gctggcggat tttagccgtg gttcattgca attgttagcg attgaagaat aatccccact
     5041 tcgttttgca gaactaagga agcggcagag atgcattttc gcgccattac ccgaatcgtt
     5101 ggactactgg tcatcttatt ttcagggacc atgattatcc ctgggctggt agcactcatc
     5161 taccgggatg gagcgggccg cgcttttacc cagacctttt ttgtcgccct cgccattggc
     5221 tctatgctgt ggtggccgaa ccgcaaagag aagggcgaac tgaaatcccg tgaggggttt
     5281 ctgatagtgg tgctgttttg gaccgtgctg ggtagtgtcg gtgcgctccc ttttatcttc
     5341 tcggaaagcc cgaacctcac gattaccgat gcgttttttg aatctttctc tggcctgacc
     5401 accaccgggg ccactacgct ggtggggctg gattcgctcc ctcatgccat cctcttttat
     5461 cgccagatgc tgcaatggtt tggcgggatg gggatcatcg tgttagcggt tgcgatactg
     5521 cctatcctcg gcgtgggtgg gatgcagctc tatcgcgcag aaatgcccgg cccgctgaaa
     5581 gataacaaaa tgcgcccgcg aattgcggaa acggcgaaaa ctctgtggtt gatttatgtc
     5641 ttgctgaccg tcgcctgtgc gctggcgttg tggtttgccg gaatggatgc ctttgatgcc
     5701 atcggccata gctttgcgac tatcgctatt ggcggcttct cgacacatga tgccagtatc
     5761 ggttatttcg acagtccgac tattaacact atcattgcta tcttcctgct gatctccggc
     5821 tgtaactacg gtctgcactt ttcactgtta agtgggcgta gtctgaaggt ttattggcgc
     5881 gatccggaat ttcgcatgtt tatcggcgta cagtttacgc tggtggttat ttgtacactc
     5941 gtactgtggt ttcataatgt ctacagttcg gcgctgatga caattaacca ggcgtttttc
     6001 caggtggtat cgatggcgac aaccgccggg tttacgactg acagcattgc ccgctggccg
     6061 ctctttttgc cggtactgct tttatgttca gcttttatcg gcggttgcgc cgggtcaacg
     6121 ggcggtggcc tgaaagtgat ccgcatcctg ctgctgttta agcaggggaa ccgtgagctg
     6181 aaacgactgg tgcatccgaa cgccgtctat agcattaagc tggggaatcg cgcactgccg
     6241 gaacgtatcc tcgaagccgt ttggggattt ttctccgcct atgcattggt gtttattgtc
     6301 agtatgctgg cgattatcgc cacgggcgtg gatgattttt ctgcctttgc ctcagttgtt
     6361 gcgacattga ataacctggg gccagggctt ggcgtggttg ctgataactt taccagtatg
     6421 aacccggtgg ctaaatggat cctgattgcc aacatgctgt ttggtcgtct cgaggtcttt
     6481 acattactgg tgctctttac cccgactttc tggcgtgaat gatggagtaa tacgtgaaaa
     6541 cattaattct tttctcaaca agggacggac aaacgcgcga gattgcctcc tacctggctt
     6601 cggaactgaa agaactgggg atccagacgg atgtcgccaa tgtgcaccgc attgaagaac
     6661 cacaatggga aaactatgac cgtgtggtca ttggtgcttc tattcgctat ggtcactacc
     6721 actctgcgtt ccaggaattt gtcaaaaaac atgcgacgcg actgaattcg atgccgagcg
     6781 ccttttattc cgtaaacctg gtggcgcgca aaccggagaa gcgtactcca cagaccaaca
     6841 gctacgcgcg gaagtttctg atgaactcgc aatggcgtcc cgatcgctgc gcggtcattg
     6901 ccggggcgct gcgttaccca cgttatcgct ggtacgaccg ttttatgctc aagctgatta
     6961 tgaagatgtc aggcggtgaa acggatacgc gcaaagaagt tgtctatacc gattgggagc
     7021 aggtggcgaa tttcgcccga gaaatcgccc atttaaccga caaaccgacg ctgaaataag
     7081 cataaagaat aaaaaatgcg cggtcagaaa attattttaa atttcctctt gtcaggccgg
     7141 aataactccc tataatgcgc caccactgac acggaacaac ggcaaacacg cagccgggtc
     7201 agcggggttc tcctgagaat tccgacagag aaagcaaaaa taaatgcttg actttgtagc
     7261 gggaaagcgt attatgaaca ccccgcgccg ctgagaaaaa gcgaagc
//
LOCUS       DADLTY010000194         7188 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.195, whole genome shotgun sequence.
ACCESSION   DADLTY010000194 DADLTY010000000
VERSION     DADLTY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.195"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            28..879
                     /gene="hchA"
                     /locus_tag="JN334_003625"
     CDS             28..879
                     /gene="hchA"
                     /locus_tag="JN334_003625"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HAY7439630.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(987..2345)
                     /locus_tag="JN334_003626"
     CDS             complement(987..2345)
                     /locus_tag="JN334_003626"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HAY7439631.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="JN334_003627"
     CDS             complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="JN334_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HAY7439632.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3149..3562
                     /gene="hiuH"
                     /locus_tag="JN334_003628"
     CDS             3149..3562
                     /gene="hiuH"
                     /locus_tag="JN334_003628"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY7439633.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3671..4675
                     /gene="msrP"
                     /locus_tag="JN334_003629"
                     /pseudo
     CDS             3671..4675
                     /gene="msrP"
                     /locus_tag="JN334_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4676..5311
                     /gene="msrQ"
                     /locus_tag="JN334_003630"
     CDS             4676..5311
                     /gene="msrQ"
                     /locus_tag="JN334_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HAY7439634.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5569..6219
                     /gene="zinT"
                     /locus_tag="JN334_003631"
     CDS             5569..6219
                     /gene="zinT"
                     /locus_tag="JN334_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAY7439635.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2032 a         1629 c         1507 g         2020 t
ORIGIN      
        1 taactaagca acaataagga atacactatg actgttcaaa caagtaaaaa cccgcaggtc
       61 gatattgctg aagataatgc gttcttccct tcagaatatt cgcttagcca atatacgagt
      121 cccgtctctg atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa acataaaatt
      181 ctggtgatcg ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc
      241 ggtaaccatc cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa
      301 ttcgaagtgg cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac
      361 aaagatgaaa aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag
      421 aaactcgcgg atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt
      481 cctggtgggc atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta
      541 cagtgggcaa tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt
      601 ctggcacttc gccacagcga taacccactg aatggttatt ctatttgcgc attcccagac
      661 gccgcagaca aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc
      721 ggcgaagagt tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta
      781 cataaggacc gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa
      841 ctggcggcgc aggaaatgct ggcagcttac gcgagttaat ccctgttaca ttgtgatgta
      901 agcgcataat caatcgcagc cacgttaata ctcacgacgt ggctgcgttt tttcacggtt
      961 aacttatggc gtactgaagc ccgatgttaa tttctttgcg gtaacgtaat tcggaacacg
     1021 ttatgcttac tgagatagtg atacgtagca cttcctccat gtaattcggc aatcgctttg
     1081 actaaagaaa ggccaagtcc ctgacctacg gaatgacgcg aattatctcc ccgccaaaat
     1141 ctacggaaga gtttttcagg ctcattaatt ttcgttccag gactggcgat atcaatatta
     1201 agagagccgt tggcatcaag aaaactggtt atatgaatac gcgatttttc tggtgaatat
     1261 cgaatggcgt taacaataag attcgataac attcgttgca gtaaaatttt atccgcaaag
     1321 atttgttgat tgcacttgac cttaaagcaa atctctttct cgtctgaaag atattcaaga
     1381 tagtccaaaa gattttcaac ttccttattg agagaaagcg aatccagttt caccaaaaca
     1441 ttgtttttgt ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc
     1501 agctcttcaa tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg
     1561 agcgtaacct gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga
     1621 tcgtcagcaa attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc
     1681 aacgcctgcc caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg
     1741 ctatcgttat aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc
     1801 gtcctgatta acaacggact taatactgag cacagtataa tggcgacaat gcaaattata
     1861 atgctattaa ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca
     1921 gtaaccgtta atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta
     1981 tcattaataa tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta
     2041 tttaacatgt catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga
     2101 atgatcaaaa tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct
     2161 ggatttaccc catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta
     2221 tcatcgcgcc atttcaactc acttgccagg ccattataga gagtccatac aattccagcg
     2281 ccagcaacag actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt
     2341 ttcattattt ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct
     2401 tttcaggaaa aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat
     2461 tggtatcact atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa
     2521 tctcgccagc tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa
     2581 tactgatatt gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt
     2641 ctaatgttga attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact
     2701 cagaaaatga aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc
     2761 tgtcatcgac agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta
     2821 acgtttgtaa gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg
     2881 cataatcatc cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga
     2941 cataacccgc ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa
     3001 taagtagaat cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt
     3061 tcattacaaa attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa
     3121 aactcattca catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc
     3181 aacggcagcc ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt
     3241 gcacattttg aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa
     3301 gaaagcggat aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat
     3361 taaggcactg tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac
     3421 cggggactat ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt
     3481 tcatattaat aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta
     3541 ttcaacctat cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag
     3601 ggaggtgact ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt
     3661 gtgatggctt atgaaaaaga agcaattttt aaaagaatca gatgttacgg ccgagtcggt
     3721 attctttatg aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc
     3781 tttgcctcac gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc
     3841 cgcccccgcc ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc
     3901 actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa
     3961 agacgatccc gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag
     4021 cggcgaagtg gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct
     4081 ggaagagcgt atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat
     4141 tggttttccg ctacacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt
     4201 cgctttcgaa acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg
     4261 cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct
     4321 cacactgatg accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt
     4381 gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa
     4441 gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt
     4501 ttacgccaac gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt
     4561 tattggttca ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta
     4621 cgccgaccag gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg
     4681 tctgacagca aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc
     4741 gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga
     4801 tattcagcat tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac
     4861 acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt
     4921 atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt
     4981 gaataatctg gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat
     5041 tatcagctgg gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa
     5101 actcggcaaa cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc
     5161 aatacattat ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgccgggct
     5221 ggctgtactg cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg
     5281 caagcaggcc cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat
     5341 cccggccgga tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt
     5401 tcgtttgtca tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca
     5461 atacacctga gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt
     5521 acaatataac attacacata atgtacatta actctggagg aaattgtttt ggcgattcgt
     5581 cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat
     5641 ggtcatcatt ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt
     5701 tttgatgata ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc
     5761 gtttaccctt tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca
     5821 gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat
     5881 atcgaaatga ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca
     5941 tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc
     6001 gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt
     6061 agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat
     6121 tcccagcaat ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg
     6181 agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt
     6241 tatttaacag aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga
     6301 caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac
     6361 tgagaaaaag gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt
     6421 tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt
     6481 ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa
     6541 caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt
     6601 ttggctcaat tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc
     6661 agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa
     6721 ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc
     6781 acagtatcga acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct
     6841 gtttaggaaa agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcggagagag
     6901 ggggatttga acccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga
     6961 gtcgaaaaat caaagagtta aaaatttttc ttggaaaaaa tgtcttaaaa tcgtacacga
     7021 aaaatcaatg aattacataa cattaaagaa gaatattcca agccttcttg agtccaccac
     7081 ctgttttcag gtgtcattca gacgtgattt gccccagatt gggaaagcac gcttttttct
     7141 cagcttattc cgttttgcct gtgtgttgaa taattccact gcagttat
//
LOCUS       DADLTY010000195         7183 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.196, whole genome shotgun sequence.
ACCESSION   DADLTY010000195 DADLTY010000000
VERSION     DADLTY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.196"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(296..1198)
                     /locus_tag="JN334_003632"
     CDS             complement(296..1198)
                     /locus_tag="JN334_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HAY7439636.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="JN334_003633"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="JN334_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HAY7439637.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVMDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="JN334_003634"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="JN334_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HAY7439638.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="JN334_003635"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="JN334_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HAY7439639.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="JN334_003636"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="JN334_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HAY7439640.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="JN334_003637"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="JN334_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
BASE COUNT         1677 a         1812 c         1875 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc atgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DADLTY010000196         7129 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.197, whole genome shotgun sequence.
ACCESSION   DADLTY010000196 DADLTY010000000
VERSION     DADLTY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.197"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..121)
                     /locus_tag="JN334_003638"
     CDS             complement(<1..121)
                     /locus_tag="JN334_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006660997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439641.1"
                     /translation="MTILNKALTRPAALWGVPLVPLLFTLCILTLAAVWSRFYW"
     gene            complement(131..430)
                     /locus_tag="JN334_003639"
     CDS             complement(131..430)
                     /locus_tag="JN334_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HAY7439642.1"
                     /translation="MTLRTIKTAATRLIAAFSALSAVPAMAASADQAFQKGSDAINWV
                     FLGLTGMSVVTISCAVAFIAYKMLVDGKAWHDLQRVFWAGVLLAGASGFGAYFAA"
     gene            complement(441..1103)
                     /locus_tag="JN334_003640"
     CDS             complement(441..1103)
                     /locus_tag="JN334_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HAY7439643.1"
                     /translation="MLTTAALFQLAMQCAPAVHPDTIHDITRTESGLNPYAIAEIVPV
                     KGGRSRVISHLPSSKDEALKIVEAIKQKKHRYSVGLMQITSTNFPQFGVSAESMFNPC
                     DNMTVAAKIITDCYQRGGTLQRALSCYYSGNFETGQRPESAFGNTSYVQRIGYVVPST
                     RAEQQTASPSSGEAPAVPSDNTVYPDSVIRGVIPAPDTTPTSIAAYPPHVVRGGLAVS
                     SD"
     gene            complement(1106..1321)
                     /locus_tag="JN334_003641"
     CDS             complement(1106..1321)
                     /locus_tag="JN334_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439644.1"
                     /translation="MPDITPAPASLLEAVFTPMTEYTHNVYSVKGDRLAMTLTKIGVA
                     QANSQGDIQLFINPDCATGKVLLYRKD"
     gene            complement(1314..1622)
                     /locus_tag="JN334_003642"
     CDS             complement(1314..1622)
                     /locus_tag="JN334_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439645.1"
                     /translation="MSMQTVRRKALRQNPYSTLGKPPLRSSRDRITRAINGTWSLREL
                     PDNVRELLIERAALRAEITTLTVKAQGLKKSDVSVLIADRLRRIDAITSLLENRKCNA
                     "
     gene            1936..2850
                     /locus_tag="JN334_003643"
     CDS             1936..2850
                     /locus_tag="JN334_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAY7439646.1"
                     /translation="MLEMFEKIEKKYWQDKIKKISKFRVIKMQDLDDAKVIEVLAKVL
                     TKKGISKVKHNSYISKVLQITTSAGYKKLNGATKWEVQQLIKVVQSVGMDMIEFFRIY
                     YEDNKEIQDAIWNDGRSEHSCKVCLYPEGSEVNTEYSALKVADQWNIFPTNDLKDELL
                     YESKRSIESIVINPKVISSTKYRIALLDDDANITESLKEILSNDYYQVDTYDNLKKLT
                     QAVATNPYDAYVLDWIIGDETSFKLVKAIRSSKNKNAMIIVLTGQLSGIKDDEIVKSI
                     HDYDIVGPYEKPIKSGIIKSNIEKYFAR"
     gene            2854..3837
                     /locus_tag="JN334_003644"
     CDS             2854..3837
                     /locus_tag="JN334_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HAY7439647.1"
                     /translation="MRNLLEYSGSLISNDSLIVAPYDCQKLSYENILPLNNGLIKNWN
                     IQKFSSDYSEIEHLNVINGMGVTLGDSIVGISVLSAIKKKNPKIKIKLIRPETAPSYV
                     EELYELASSVIDELHYMPFHIQKLPCTALNIDVGNQLYSPDFHIMEMHDYFIQGLGVK
                     LDDVNHNELSNKWLGNIDLGAPVFENYTLFAPIASTKIRSIPSKFYYDIVDMLSRREG
                     KTVLGFVDVNHKNYINISKYSPHTKDFIALIKYASNVYTCDSSALHIAAGFGVPTECV
                     FNTIPPELRTKYYSKCKSIYVGNKRLEKIQSSEDFQLVKMVENNYREYLYG"
     gene            3830..4318
                     /locus_tag="JN334_003645"
     CDS             3830..4318
                     /locus_tag="JN334_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY7439648.1"
                     /translation="MVKIISFLSLMLLSISAYSNIGDIVIDGSIANANSDKGYVITEK
                     QFDSFPRDTIVTTTPWTPTGKKVKFKGVDLSYVLKLAGANGAKLKFHALNDYEIIVDM
                     NDVEKYNILLATEMDGKRLQIRDFGPYFLVYPLDEHYIELNTPHYLARFIWQIDKITV
                     LK"
     gene            4315..6033
                     /locus_tag="JN334_003646"
     CDS             4315..6033
                     /locus_tag="JN334_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HAY7439649.1"
                     /translation="MKKNFFIILVALLLLIAFTVSSVVKYTSLLSEKNLYTVAGTQEN
                     YGFSVAKFIIQLNELYTLINSDNTIDDVRLKFDILYSRLNVIYVKSEATAPLYKQQGY
                     EETIDSINKKLEDIDGLLSHNHPDYKKISTIIADIKPLTKTVTNLADMAEIAQRNDAL
                     KDFRSKRQQLWTLLFITGGLISLLLFVLFIYISKINRLLLSERAAFASKNAFLGMVGH
                     ELRTSLQAIVSIIDVVTNNLSGGIKSGQIERLETAVSKMERQLNDLAEFAKIDNGSVE
                     IKNTYNSLQAIVTNAVQDCIAIYEKKDVTVKIKNNNDAVIFTDALRLNQVIENLTSNA
                     IKYTERGEVNVDYFIEKGKVLNIVISDTGKGIPKDKLKFIFKPFTRVVDSKSTVPGFG
                     MGLAIVAGIIRLLKGTIHISSEVNVGTTVTVRIPIKLGDSSLATAEVSASAPGDVFGV
                     LSLLVIDDNEMACSSLSSLLTNAGYIVEATTSPERALEKLLRKPYDVVLSDLQMPGMT
                     GDELYATVSSSQNPNRSTPFIFISAYSDAHSIGQAPLLTKPVRLKDINLKIHEVMSNQ
                     GRVKSE"
     gene            6297..6506
                     /locus_tag="JN334_003647"
     CDS             6297..6506
                     /locus_tag="JN334_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439650.1"
                     /translation="MLLTLRGMFFSYFSDTRELNTVVAKLYPLKMSQMPLYAVGVVPA
                     RRQPQPGRISALAGRRAAYERVPDN"
     gene            6715..6849
                     /locus_tag="JN334_003648"
     CDS             6715..6849
                     /locus_tag="JN334_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439651.1"
                     /translation="MGALSDVAEPARPSGRCKSGHSPEPVHRVGDEEVMCLQDLLKDL
                     "
     gene            complement(6892..7129)
                     /locus_tag="JN334_003649"
                     /pseudo
     CDS             complement(6892..7129)
                     /locus_tag="JN334_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         2083 a         1437 c         1700 g         1909 t
ORIGIN      
        1 gccagtaaaa acgtgaccag acggccgcca gggtaaggat gcacagggtg aacagcaggg
       61 ggacaagcgg gaccccccac agcgccgccg gtcgggtcag cgccttgttg aggatggtca
      121 tgctgcctcc ttaggcggcg aagtaggcac cgaacccgga cgccccggcg agcaggacac
      181 ccgcccagaa cacgcgctgg agatcgtgcc aggccttgcc gtcaacaagc atcttgtagg
      241 cgataaaggc caccgcacag ctgatagtga cgactgacat accagttagg ccgaggaaga
      301 cccagttaat ggcgtcactg cctttctgaa atgcctggtc agcgctggcc gccattgctg
      361 gaacagcaga cagggcggaa aatgcggcaa tcaggcgggt tgcagcggtt ttgatagtac
      421 gtaaagtcat gatctcttcc ttaatcagaa gacacagcga ggccgccgcg cacgacatgt
      481 ggcggataag cagcgatgct ggtcggggtg gtgtcgggtg ccggaattac gccgcgtatg
      541 acgctatccg ggtaaacagt gttgtcggac gggacggcag gcgcttcccc ggatgaggga
      601 gatgctgtct gctgttctgc ccgcgttgag ggcacaacgt agccaatacg ctgtacgtag
      661 cttgtgttgc cgaacgcgct ttccgggcgc tggccggttt caaagttgcc tgagtaatag
      721 cagctcaggg cgcgctgtaa tgttcctcct cgttgatagc agtcggtgat gattttcgcg
      781 gcaacagtca tgttgtcaca ggggttaaac atggattcgg cgcttacgcc gaactgcggg
      841 aagttggtgc tggttatctg catcagccct actgaataac ggtgcttttt ctgctttatg
      901 gcctccacaa tttttagcgc ctcgtctttc gacgaaggca gatgagaaat cacgcggctc
      961 ctgccgccct ttaccgggac aatctcagcg atggcatatg gatttaagcc ggattcggtg
     1021 cgggtgatgt cgtggatggt atcggggtga acggcgggag cacactgcat tgcgagctga
     1081 aacaacgcgg cagtggttaa catagtcagt cctttctgta gagcagtacc ttaccggtgg
     1141 cacagtccgg gttgataaat aactgaatgt cgccctggct gttagcttgg gcaacaccga
     1201 tttttgtaag cgtcattgcc agccgatcac ccttcaccga atacacgttg tgcgtgtact
     1261 cggtcatggg ggtgaatacg gcttcaagca gtgaggccgg agcaggcgtg atgtcaggca
     1321 ttacatttcc tattttccag cagtgaggtg attgcatcga tacgtcgcag cctgtcggca
     1381 ataagtacgg atacatcgga ttttttgagt ccctgcgctt tcacagtcag ggtagtgatt
     1441 tctgcccgca gggctgcccg ttcaattagc aattctcgga cgttatccgg cagctccctg
     1501 agcgaccagg tgccattaat ggcacgcgtg atacggtcac gggaggagcg tagcggcggc
     1561 ttgccgagag ttgaataagg attctgacgc agcgccttac ggcggacggt ttgcatgctc
     1621 atggctgttt ctcctgggta tgagtaagcc acccgcgtgc ggtgcacagt gggagacagg
     1681 gaaggattaa acgggaggct taacgataca gaggagtgag agtgttaccc attacaggcg
     1741 cggcacgtca tctctttacg cagtcacaga aaacacctcc ttatcctgac cggggaacgc
     1801 cgctgctagg ttattaaaga gctaatgatt tgattaatac aatagtgctt ataagttggg
     1861 tattcaataa aattataact tatagtatcg atttaagaat gattccaatt tttaggactt
     1921 tttcagctgt gtaaaatgtt ggagatgttt gaaaaaattg aaaaaaaata ctggcaggat
     1981 aaaatcaaga aaatctccaa attcagagta ataaaaatgc aagatctcga tgatgcaaaa
     2041 gtgatagagg tactggcgaa ggtattgacc aaaaaaggta tttccaaggt aaagcacaat
     2101 agttacataa gtaaggtgct acagatcact acatcagcgg gctataagaa gttgaatggt
     2161 gcgaccaagt gggaggttca gcaactcata aaagtagtcc aatccgttgg catggatatg
     2221 attgagtttt tccgtattta ttatgaggat aataaagaaa ttcaagatgc gatctggaac
     2281 gatggccgca gtgaacactc ttgtaaggta tgcctctatc ctgaaggttc ggaagtaaac
     2341 acagaatact cagcactgaa agtagctgac caatggaaca tttttccgac aaatgattta
     2401 aaagatgaac tgctttatga atcaaaacga agcatagaaa gtattgtcat aaatccaaag
     2461 gtaattagca gcactaagta tcgaattgca ctacttgatg acgatgctaa tatcaccgaa
     2521 agcttaaaag aaatcctttc gaacgattat tatcaagtag atacttatga taacttgaaa
     2581 aaacttactc aagctgtcgc aacaaatccg tatgacgcat atgttttgga ctggatcatc
     2641 ggtgatgaaa caagctttaa gttggttaaa gctataaggt cgtcaaaaaa taaaaatgca
     2701 atgataatcg ttcttacagg tcaactatct ggtataaaag atgatgaaat tgtaaagtca
     2761 attcatgatt atgacattgt gggaccatac gaaaagccga ttaaatcagg catcatcaaa
     2821 tctaacattg aaaaatattt cgccagatag attatgagaa atttacttga atattccggt
     2881 agcttgataa gtaacgatag tttgatcgta gcaccttacg attgtcagaa gctaagctat
     2941 gaaaatattt taccattaaa caatggtctt ataaaaaact ggaatataca aaaattctca
     3001 agtgattact ctgaaataga acacctaaat gtgatcaatg gcatgggagt gacattaggt
     3061 gattctatcg tcgggatttc tgtgctgtca gcaatcaaaa aaaagaatcc caagattaaa
     3121 attaagctta ttcgaccgga gactgcaccg tcatatgttg aggaactgta tgaattagca
     3181 tcctcggtta tagatgagct tcattacatg ccttttcata ttcagaaatt gccttgtact
     3241 gcattgaata ttgatgtggg aaatcagcta tattctccag atttccatat tatggaaatg
     3301 catgattatt ttatccaagg tcttggcgtg aaacttgatg atgttaacca taatgagcta
     3361 tctaataaat ggttaggcaa tattgatctc ggagcgccag tttttgaaaa ctacacactt
     3421 tttgcaccta ttgctagcac aaaaataaga agtataccgt cgaagttcta ttatgacata
     3481 gttgatatgc tttctaggcg cgaaggaaaa acggtactag gtttcgtgga tgtgaatcat
     3541 aaaaattata ttaacatatc aaagtattca ccccatacga aagatttcat agcgctaatt
     3601 aagtatgcta gcaacgttta tacttgtgac tcctctgcat tgcatatagc cgctggattt
     3661 ggtgtaccta ctgaatgcgt gtttaataca ataccgccag aattaagaac aaagtattac
     3721 agtaagtgta agtcgattta tgttgggaat aaaagattag aaaaaattca gagtagtgaa
     3781 gatttccaac tggttaaaat ggtggaaaat aattacaggg agtatcttta tggttaaaat
     3841 aatatcattc ttatccctaa tgcttttgtc aattagtgca tatagcaata ttggggatat
     3901 agttatagat ggttcaatcg ctaatgctaa cagtgataaa ggttatgtga ttactgaaaa
     3961 gcagtttgat tcgttcccac gggataccat cgtaacgaca acaccttgga cgccaaccgg
     4021 taaaaaagta aaattcaagg gcgtcgacct aagttatgtt ttaaaactcg ctggcgctaa
     4081 tggagcaaag ctaaaattcc atgctctgaa tgattatgaa atcattgtag acatgaatga
     4141 cgttgaaaaa tataacattt tattggctac agaaatggat ggaaaaaggc ttcaaataag
     4201 ggattttgga ccttattttt tggtatatcc tttagatgaa cattatattg agcttaacac
     4261 gccacactat cttgcacggt tcatttggca gatagacaaa attacggtgc tcaaatgaaa
     4321 aaaaatttct ttatcatttt ggttgctctg cttttgttga tagcattcac tgtgtctagt
     4381 gttgttaagt acaccagtct tttatcggag aaaaatttat acaccgtcgc tggcacccaa
     4441 gaaaactacg gtttctcagt ggcaaaattc attatacagt tgaatgagct ttacacgcta
     4501 atcaatagcg ataatactat tgacgatgtt aggcttaaat tcgatatcct ttattctcgt
     4561 ttgaatgtta tttatgtaaa gagtgaggcg actgcacctc tatataaaca gcaaggctat
     4621 gaagaaacaa ttgattcgat aaacaaaaag cttgaagaca tagacgggct gctttcgcat
     4681 aatcaccctg attataaaaa aataagtaca atcatagctg atataaaacc gctaactaaa
     4741 accgttacaa atcttgctga tatggctgaa atagcacaaa ggaatgacgc tcttaaagat
     4801 ttcaggagca aacgtcagca gttatggact ctgcttttta ttacaggtgg gttgatttcc
     4861 ttacttcttt ttgtcctatt tatttacata agtaaaatta accgattgtt actatcagaa
     4921 cgggctgctt ttgccagtaa aaatgcattt ttaggtatgg taggccatga actcagaaca
     4981 tctttacagg caatagtgtc tatcattgat gtcgtaacca ataatctgag tggagggatt
     5041 aaatctggtc agatagaacg acttgaaact gctgtttcga aaatggagcg ccagttaaat
     5101 gatttggctg aatttgcaaa aattgacaac ggatctgtag agataaaaaa cacctataat
     5161 agcttgcaag caattgttac taacgctgtg caggattgca ttgctattta tgaaaaaaag
     5221 gatgtcacag ttaaaataaa aaacaataat gatgcagtga tttttacaga tgctctacgc
     5281 cttaatcaag taattgaaaa cttaaccagt aacgctatta agtacactga gcgcggggaa
     5341 gttaatgtag attactttat agaaaaaggt aaggtattaa atattgtcat ttctgatacc
     5401 ggaaagggaa tccctaaaga caaactaaag ttcatattta aaccatttac acgagttgta
     5461 gattcgaaat ccactgtacc tggatttggg atgggacttg ctattgttgc agggataatc
     5521 agactattaa aaggtactat tcatatatct agtgaagtaa atgtaggtac aactgttaca
     5581 gtgagaatac ctatcaagtt gggagatagc agccttgcaa cagctgaggt atctgcttca
     5641 gctcccggag atgtcttcgg cgttcttagc ttgcttgtta ttgatgataa tgaaatggcc
     5701 tgttcgtctc tttccagtct gcttactaat gctggataca ttgtcgaggc tacaacctcg
     5761 cctgaacgag ccctggaaaa gcttctgcgt aaaccttatg acgttgtcct gagtgattta
     5821 caaatgccag gcatgacagg tgatgagctg tatgcaactg ttagtagcag tcagaatcct
     5881 aatcgatcca cgcctttcat attcatcagc gcatacagtg atgcacactc tatcggacag
     5941 gctcctttgt taactaaacc tgtcaggtta aaagatataa accttaaaat acatgaggtt
     6001 atgtcaaatc aaggacgagt caaaagtgag taggcattgt cgtaatattt gcgattatag
     6061 ctttggtgtt ctcgatcgtt gcgaattttg aaaatttgtg gagtcaaatg gcgccgaaag
     6121 cccggcgtag ccggttacct ggctataaac tctgccaccc taccccctgc cctgattagc
     6181 cttaacccgc ttacagcaat gaagaagtaa aagtaatccc ttttaacccc ggtgagcggg
     6241 ctttacggcg tttacgacgt aaagggcgtg atttccgggg ccttagcggc ggcagcttgc
     6301 tgctgacgct tagggggatg ttcttcagtt atttcagcga caccagggaa cttaacacgg
     6361 tagttgcaaa gctatacccg ctaaaaatgt ctcagatgcc tctgtacgcg gtcggagtgg
     6421 tcccggcaag gcgtcagccg cagccggggc gtatctccgc gttagcgggc cgaagggccg
     6481 cttacgagcg tgtacccgac aactgaccgc agctccaccc acagcgatga agaagagtag
     6541 ttacaacacc catcattaaa agcgacacta ctcccggcct tcggccgggg gcggcggcgc
     6601 ttttcagtgg tggggagggg cacccggccg gcaaaccggc gccggtttta tgatgagctt
     6661 tcttttgaat gctgtaacta atttggttct ccggtcgggg cccttcgcga agcgatggga
     6721 gctctttctg atgttgccga accggcgcga ccgtcaggtc gctgcaagag cgggcattct
     6781 ccggagcctg tccacagggt aggcgatgaa gaggttatgt gtttacagga tttattaaag
     6841 gatctttaaa agagatacag aactggtgtt taaaaggaac tacgcgccct gaaagaccga
     6901 cggcaggctc agaagatgct ccagcgtggc catagtacgt tcaccgaaga cgtgtgcaac
     6961 aaccgtcctc cgtatcctgt catacgcgta aaacaaccag cgctggcgtg atttagcgcc
     7021 gacgtaaccc cactgttcgt ccatttccgc gcaaacaatg acgtcactgc ccggttgtat
     7081 gcgtgagttt accgactgcg gcctgagagg cggtgtaagt gaactgtag
//
LOCUS       DADLTY010000197         7039 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.199, whole genome shotgun sequence.
ACCESSION   DADLTY010000197 DADLTY010000000
VERSION     DADLTY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7039
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.199"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..153
                     /locus_tag="JN334_003650"
     CDS             <1..153
                     /locus_tag="JN334_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HAY7439652.1"
                     /translation="AFPDNEGHVSVRYAAANNLSVIGATKEDKQRISEILQETWESAD
                     DWFVSE"
     gene            443..697
                     /gene="bssS"
                     /locus_tag="JN334_003651"
     CDS             443..697
                     /gene="bssS"
                     /locus_tag="JN334_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HAY7439653.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            812..1930
                     /gene="solA"
                     /locus_tag="JN334_003652"
     CDS             812..1930
                     /gene="solA"
                     /locus_tag="JN334_003652"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HAY7439654.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            1978..2091
                     /locus_tag="JN334_003653"
     CDS             1978..2091
                     /locus_tag="JN334_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HAY7439655.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2352..2918
                     /locus_tag="JN334_003654"
     CDS             2352..2918
                     /locus_tag="JN334_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAY7439656.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2922..3497
                     /locus_tag="JN334_003655"
     CDS             2922..3497
                     /locus_tag="JN334_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAY7439657.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3539..4591)
                     /locus_tag="JN334_003656"
     CDS             complement(3539..4591)
                     /locus_tag="JN334_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAY7439658.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4816..5736
                     /locus_tag="JN334_003657"
     CDS             4816..5736
                     /locus_tag="JN334_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HAY7439659.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5908..6105
                     /locus_tag="JN334_003658"
     CDS             5908..6105
                     /locus_tag="JN334_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HAY7439660.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            6129..>7039
                     /locus_tag="JN334_003659"
     CDS             6129..>7039
                     /locus_tag="JN334_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7439661.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGII"
BASE COUNT         1829 a         1669 c         1667 g         1874 t
ORIGIN      
        1 gcgtttcctg ataatgaagg ccacgtatcg gtacgttatg ccgcagcgaa taatttatcg
       61 gttattggcg caacaaaaga agataaacag cgcattagcg aaattctcca ggaaacgtgg
      121 gaaagcgccg atgactggtt tgtcagcgaa taatatgcag tgatttttgt ttttgccgga
      181 tcgccccggc ttttttattt tacttacgaa ttttgagtac aaaatttcta cacttcctca
      241 aaataattgc acaatatgct aaaaaaatat gaatgacatg ctgttttatt gttcatataa
      301 atcttaaaag cagaaattta tctgttcccc cctttaaaaa ttcggtgaat acccttactt
      361 attggtatac tgaagttgct tcagataaaa acgcattgaa cctcgaataa cgttgtctag
      421 taacacgaat tagggggcca tgatggaaaa aaataatgaa gtcattcaga ctcatccgct
      481 cgtagggtgg gacatcagca ccgttgatag ctatgatgcg ctgatgttgc gtttgcacta
      541 ccagacccca aataagtccg agcaggaagg gactgaagtt ggtcagacgc tctggttaac
      601 cactgatgtt gccagacaat ttatttcgat attagaagca ggaatcgcca aaattgaatc
      661 cggtgatttc ccggtaaacg agtatcggcg tcattaacat gacatgacca tccacccaaa
      721 aggcacctca ccggtgcctt taccatttct gggttgtata tctgcattca gttaattact
      781 ctgcttaaac catgagagca gagagaaaaa aatgaaatac gatctcatca ttattggcag
      841 cggttccgta ggcgctgccg ccgggtatta tgcaacccgc gccggtttaa acgtgctaat
      901 gaccgacgcc catatgccac cgcatcaaca cggcagccac cacggcgata cgcgattaat
      961 tcgccatgct tatggtgaag gcgaaaagta tgtcccgttg gtcctccgcg cgcaaacgct
     1021 gtgggatgaa ctttcccgcc acaacgaaga tgatcccatt tttgtacgct ctggtgtcat
     1081 taacctcggc ccggctgact ccgcatttct cgccaacgtc gcccacagcg ccgaacagtg
     1141 gcaactcaac gttgaaaagc tcgatgcgca agggattatg gcccgctggc cagaaatacg
     1201 cgtcccggac aactacatcg gcttatttga gactgattcc ggttttttgc gcagcgaact
     1261 ggcgattaaa acctggatcc aactggcgaa ggaagcgggc tgtgcgcaac tgttcaactg
     1321 cccggtcacc gcaattcgtc atgacgatga tggcgtaact attgaaacgg ttgacggtga
     1381 gtatcaggcg aaaaaagcga ttgtctgcgc gggaacatgg gtaaaagacc tgctcccgga
     1441 gctgcctgtc cagcccgtac gtaaagtatt tgcctggtat caggccgatg gccgctatag
     1501 cgtgaagaat aaattcccgg cgtttaccgg tgaactgccc aatggcgatc aatattatgg
     1561 ttttccggca gaaaacgacg cgttgaagat tggcaaacat aacggaggcc aggttatcca
     1621 ttcagcggat gaacgtgttc cgtttgcgga agtggccagt gatgggtcag aagccttccc
     1681 gttcttgcgc aatgtattgc cgggtatcgg ttgctgcctg tacggcgctg cctgcaccta
     1741 tgataattcg cctgacgaag attttattat cgatacccta cccggccacg ataatacact
     1801 gctcattacc ggcctgagtg ggcacggttt taaatttgcg tcagttttag gggaaatagc
     1861 tgccgatttt gcgcaagacc aaaaaagcga ttttgatttg acgccattca ggctttcccg
     1921 cttccaataa tcatcattac ggcctccaga atgaggccgt ttttacagga gtatttaatg
     1981 cgtcggctgt tgcactatct catcaataat attcgcgagc atctgatgct ttatcttttt
     2041 ctctggggat tgttggcaat tatggatctg atctatgtgt tttattttta gttttccgca
     2101 ctgacataaa ctgacaaata tttaatctcc ctttcattta gcgtgaaaaa ttgaatgaga
     2161 ttattcctgc ttctacagca ctcaccagac atctctgatg ctgaacccac taaacttatg
     2221 aagattcttt cattattaat tcaaaattat tgaaataaaa ataaatatca ttcaatttgt
     2281 tatttttgtg ttgcaattca ttctccgaca cgccattttc tttctctcgc aaattcgtat
     2341 tggatgcact tatgtcattc acaaataccc ctgaacgcta tggtgttata tctgcggcct
     2401 tccattggct aagcgcaatt attgtctacg gcatgtttgc tttaggtctg tggatggtca
     2461 cgctcagtta ttacgatggc tggtatcaca aagcacccga actgcataaa agcatcggta
     2521 ttttgttaat gatggggctg gttattcgcg ttctgtggcg tgtcatatct ccaccgccgg
     2581 gtccgctgcc gagttattcg cccatgactc gtcttgccgc aaaagctgga catctcgcat
     2641 tgtgcctatt gctgttcgcc atcggcatca gcggctatct gatctcaact gccgatggta
     2701 aaccgatcag cgtttttggc tggtttgacg tccccgcgac ccttgccgac gctggcgcac
     2761 aggcagactt tgccggtgcc ctgcattttt ggctcgcgtg gagcgtcgta gtactgtctg
     2821 ttatgcacgg atttatggcc ctgaagcatc atttcatcga taaagacgac actctgaagc
     2881 gcatgctggg aaagtcgtca tctgactatg gagtataaaa aatgaaaaaa agcctgcttg
     2941 gtttaacctt cgcgtccctg atgttctctg ccggttcagc ggttgccgcc gattacaaaa
     3001 ttgacaaaga aggtcagcac gcctttgtta acttccgcat acagcatctt ggctatagct
     3061 ggttatatgg cacctttaaa gatttcgacg gtacttttac ctttgatgaa aaaaatccgg
     3121 ctgccgataa agtgaatgtg acaattaaca ccaccagcgt cgatactaac cacgccgaac
     3181 gcgataaaca tcttcgcagt gcggattttc tcaataccac aaaatatcca caggcaacat
     3241 tcacctccac cagcgtgaag aaagacggtg acgaactgga tattaccggc gatctgacgc
     3301 tgaatggcgt aaccaaaccc gtcacgctgg aagcgaaatt aattggtcag ggcgacgacc
     3361 catggggcgg taaacgtgca ggctttgagg ccgaaggcaa aattaagctc aaagacttca
     3421 atatcaagac agatttaggt ccagcttctc aggaagtgga tctgattatt tcagtggaag
     3481 gcgtacagca gaagtaagag tgaaatgttg ccggatggca cgcatccggc aatgatattt
     3541 attctgttgg atcaggaata cccagtgttg tattcagacg tccacgagac ttattaaaga
     3601 tcttattgcc attttcacgt cctgcccgac ggcgtcgctg ttcttctggc ggtaacgcgc
     3661 tttcttcgca gcaaatctca ctacaacaac ctttgtattt ttccgcgcat actggacact
     3721 gaataaacag caggtggcag ccatcatttt tacagttggt atggctgtcg cacggcgcgc
     3781 cgcactggtg gcaatgcgcg ataatctcat ccgatatacg ttcgcccatc cgctcgtcaa
     3841 aaacaaaatt tttgccaata aaacgcaccg gcaagccctg ctcgcgcgcc ttacgggcgt
     3901 attcaataat tccgccctcg atatgccaga ctttgttgaa tccgttatgt ttcatccagg
     3961 cactggcctt ttcacaacga atgccgccgg tgcagtacat gacgattttt ttatctttat
     4021 gtgcctgcat catctcgact gcttttggca gctgctcacg gaaggtatct gccggaattt
     4081 cgagcgcgtt ttcaaagtgc cccacttcat actcatagtg gttacgcatg tcgataaata
     4141 gtgcatcggg atcgtcaagc atggcgttca cttccgccgc ttgcagatac tcaccaacat
     4201 tgctggcatc aaagtgagga tcgtcaatac cgtcggcaac aatgcgatcg cgtaccttca
     4261 tgcgcagtac ccagaaggat ttcccgtcat catccaacgc gatattcagg cgtaaaccct
     4321 ctaaagccgg gtcgaaggca tagagctgcg cgcgaaatgt ttcaacattg ctcgcaggta
     4381 cgctgatttg cgcgttaatg ccctcatgcg ccagatacac tcgcccaaaa acattcagcg
     4441 cggtaaacag ctgatataaa gcgtcacggg tcgccttagg atcggcgatg tggaaatact
     4501 tgtaaaacga aatggtggtt cgcggttcgc tctcagccaa cattttggct tttaacgcgt
     4561 cgttggaaat gcggttgtgt aacactggca tggtgtacgg ttcctgcgag atgggaaagt
     4621 aaaaatccgc ggcatgatat agcaattatc gataattaac atccacacat tttacgctac
     4681 atttgcgcat taaaaattat ttgttattta caagcgcggc aatttcgccc agtcttcagc
     4741 cacaaatttt ggttgcgggc gaaaaaatgc gacaatacat acaattgccc gtataggttg
     4801 aaaaacagga ttgatatgac gaatctaccc aagttctcca ccgcactgct tcatccgcgt
     4861 tattggttaa cctggttggg tattggcgta ctttggttgg tcgtgcaatt gccctacccg
     4921 attatctacc gcctcggttg tggattagga aaactggcgt tacgttttat gaaacgacgc
     4981 gcaaaaattg tgcatcgcaa cctggaactg tgcttcccgg aaatgagcga acaagaacgc
     5041 cgtaaaatgg tggtgaagaa tttcgaatcc gttggcatgg gcctgatgga aaccggtatg
     5101 gcgtggttct ggccggaccg ccgaatcgcc cgctggacgg aagtgatcgg catggaacac
     5161 attcgtgacg tgcaggcgca aaaacgcggc atcctgttag ttggcatcca ttttctgaca
     5221 ctggagctgg gcgcgcggca gtttggtatg caggaaccgg ggattggcgt ttaccgcccg
     5281 aacgataatc cactgattga ctggctacaa acctggggcc gtttgcgctc aaataaatcg
     5341 atgctcgacc gcaaagattt aaaaggcatg attaaagccc tgaaaaaagg cgaagtggtc
     5401 tggtacgcac cagatcatga ttacggcccg cgctcaagcg ttttcgtccc gttatttgcc
     5461 gttgagcagg ctgcgaccac gaccggaacc tggatgctgg cacggatgtc cggcgcatgt
     5521 ctggtgccct tcgttccacg ccgtaagcca gatggcaaag ggtatcaatt gattatgctg
     5581 ccaccagagt gttctccgcc actggatgat gccgaaacta ccgccgcgtg gatgaacaaa
     5641 gtggtcgaaa aatgcatcat gatggcacca gagcagtata tgtggttaca ccgtcgcttt
     5701 aaaacacgcc cggaaggcgt tccttcacgc tattaaatct cccatgccgg atgcttcaga
     5761 atggcatccg gcattaccac agcaaatccc cctgatttag cgataaaagc tctctggatt
     5821 gcgccccctg gaagtcgggc gcataattag tgtgtttatc ttttcttctt atgttcaccg
     5881 cgcctggcgc accaacagcg gattgctatg tcaccctgtg aaaatgacac ccctataaac
     5941 tggaaacgaa acctgatcgt cgcctggcta ggctgttttc ttaccggggc cgccttcagt
     6001 ctggtaatgc ccttcttacc cctctacgtt gagcagcttg gtgttaccgg ccactccgcc
     6061 ctgaatatgt ggtccggtat tgtcttcagt ataatgggtt cctaaacaca tcattttagg
     6121 tattgaatat gagacgggca atcagctaca tcagattttc aagtgaacga caactgaaag
     6181 gggactctgt acgccgacaa tctaagctgg ttactgactg gcttgataag aaccctgagt
     6241 tttacttgga ttcatccttg agctttaaag atttggggaa aagtgctttt agtggtaagc
     6301 acctgaaagg tggtctaggt gactttttaa ccgctatcga gaaagggctt gttaaagctg
     6361 gtgatacgct actaatcgag agtttagacc gtctgtcacg tcaggatatt gatattgctt
     6421 ctgagctatt acgccgtatc ttaagggctg gtgtagatgt agtcacactg agcgatggag
     6481 aacattacac acgagaaagt ttaaaagacc cgttagccct gattaaaagc attctgataa
     6541 tgcagcgggc gcatgaggaa agtttgagaa agtctgagcg tgtccaagct gcatggaacc
     6601 gtaaaaaaga actcatctcc gaaggtatca aggtatctag gcgttgtcct gcatggttaa
     6661 ggctgaatga tgaccgtcga acattcacaa tcattcctga taaagtggaa gttgttaaac
     6721 gagcatttga cctgagatta cagggtttaa gcttttgggc tatcaccaga actttaaacg
     6781 atgaaggtca cttgtcattg aatcaataca cacccaaaca aaaggggtgg tctgatactg
     6841 ctgtaaagaa actgctcaga aatcgtgctg tgattggatg tttcacacct gctggacgtg
     6901 aagaagttca gggttactac cccgcaataa tcagtgagtc actattctat cgagtacagc
     6961 aactgaacac agggcaatat ggtagggctt ctgtatcaag taatccgcta tcagtcaact
     7021 tgttcagggg tatcatcaa
//
LOCUS       DADLTY010000198         7008 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.200, whole genome shotgun sequence.
ACCESSION   DADLTY010000198 DADLTY010000000
VERSION     DADLTY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.200"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..1174
                     /locus_tag="JN334_003660"
     CDS             <1..1174
                     /locus_tag="JN334_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY7439662.1"
                     /translation="FQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEE
                     WQPQNRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTL
                     DQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLK
                     KNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGN
                     YDKLISAMDFYPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHW
                     FDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLY
                     KLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1226..2383
                     /locus_tag="JN334_003661"
     CDS             1226..2383
                     /locus_tag="JN334_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY7439663.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2349..2471)
                     /locus_tag="JN334_003662"
     CDS             complement(2349..2471)
                     /locus_tag="JN334_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439664.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            2674..4359
                     /locus_tag="JN334_003663"
                     /pseudo
     CDS             2674..4359
                     /locus_tag="JN334_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4397..6757
                     /locus_tag="JN334_003664"
     CDS             4397..6757
                     /locus_tag="JN334_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY7439665.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            6802..>7008
                     /locus_tag="JN334_003665"
     CDS             6802..>7008
                     /locus_tag="JN334_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439666.1"
                     /translation="MEIIMRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDL
                     RYMIYPHAHPKDQVNLWLQIHTGSL"
BASE COUNT         2058 a         1526 c         1490 g         1934 t
ORIGIN      
        1 attccagaat catggttatt acactgcagc agtaggtaaa tggcacttgt caaaaatcag
       61 taatgtgccg gtaccggaag ataaacaaac gcgtgactat catgacaact tcaccacatt
      121 ttctgcggaa gaatggcaac ctcaaaaccg tggctttgat tactttatgg gattccacgc
      181 tgcaggaacg gcatattaca actccccttc actgttcaaa aatcgtgaac gtgtccccgc
      241 aaaaggttat atcagcgatc agttaaccga tgaggcaatt ggcgttgttg atcgtgccaa
      301 aacacttgac cagcctttta tgctttacct ggcttataat gctccgcacc taccaaatga
      361 taatcctgca ccggatcaat atcagaagca atttaatacc ggtagtcaaa cagcagataa
      421 ctactacgct tccgtttatt ctgttgatca gggtgtaaaa cgcattctcg aacaactgaa
      481 gaaaaacgga cagtatgaca atacaattat tctctttacc tccgataatg gtgcggttat
      541 cgatggtcct ctgccgctga acggggcgca aaaaggctat aagagtcaga cctatcctgg
      601 cggtactcac accccaatgt ttatgtggtg gaaaggaaaa cttcaacccg gtaattatga
      661 caagctgatt tccgcaatgg atttctaccc aacagctctt gatgcagccg atatcagcat
      721 tccaaaagac cttaagctgg atggtgttcc cttgctgccc tggttgcaag ataagaaaca
      781 aggcgagcca cataaaaatc tgacctggat aacctcttat tctcactggt ttgatgagga
      841 aaatattcca ttctgggata attaccacaa atttgttcgc catcagtcag acgattaccc
      901 gcataacccc aacactgagg acttaagcca attctcttat acggtgagaa ataacgatta
      961 ttcgcttgtc tatacagtag aaaacaatca gttaggtctc tacaaactga cggatctaca
     1021 gcaaaaagat aaccttgccg ccgccaatcc gcaggtcgtt aaagagatgc aaggcgtggt
     1081 aagagagttt atcgacagca gccagccacc gcttagcgag gtaaatcagg agaagtttaa
     1141 caatatcaag aaagcactaa gcgaagcgaa ataactaaac cttcatgcgg cggatttttc
     1201 cgccgcctta ttgagcgaga tagcgatgca cgttacagcc aagccctcca gttttcaatg
     1261 taatctcaaa tgtgattact gtttttacct tgaaaaagag tcgcagttta ctcatgaaaa
     1321 atggatggat gacagcactc tgaaagagtt catcaaacaa tatatcgaag cgtctggcaa
     1381 tcaggtctat tttacctggc aaggcggtga acccactctg gctggcctgg attttttccg
     1441 taaagttatt cactatcaac aacgctatgc aggccaaaaa cgtattttta atacattaca
     1501 aacgaatggc attttattga ataatgaatg gtgtgccttt ctcaaagaac atgaatttct
     1561 ggttggtatc tcgatcgatg gcccccagga gttacatgac cgttacagac gcagtaattc
     1621 aggtaacggt acttttgcaa aagtgatagc agccatcgag cgtctgaaat catatcaagt
     1681 agagtttaat acgttaaccg tcattaataa cgttaatgtc cattatcctc ttgaggttta
     1741 tcatttttta aaatctatcg gcagtaaaca tatgcaattt atcgaattgc tagaaaccgg
     1801 gacgccgaat attgatttca gtggtcatag tgagaacaca ttccgtatca ttgatttttc
     1861 tgtgcctccc acggcttatg gcaagtttat gtcaaccatt tttatgcaat gggttaaaaa
     1921 cgatgtgggt gaaattttca ttcgtcagtt tgaaagcttt gtcagccgtt ttttggggaa
     1981 tgggcataca agttgtattt tccaggagtc ctgcaaggat aatctggttg ttgaaagtaa
     2041 tggagacatt tacgaatgcg accattttgt ctatccacag tacaaaattg gaaacattaa
     2101 taaatctgaa ctcaaaacga tgaacagtgt acaactgaca gcgcaaaaaa aacggatttc
     2161 agcgaaatgt cagcaatgtg catataaacc tatctgcaat ggcggttgtc ctaagcatcg
     2221 tattactaaa gtaaacaatg agactgtttc ttatttttgc gaaggttata aaatactttt
     2281 ttcaaccatg gtaccttata tgaacgccat ggttgagtta gctaagaaca gagtaccgct
     2341 ttaccacatt atggatgttg caaaacaaat ggagaataat taataacgct ctcttttaac
     2401 tccattctta gcctccttct tatgacctta acaaggcacc cgacataaat tcctgtcggg
     2461 tgtttcgtca tcatcatctt cccttgcgaa aatcctggct tacgtggtat gcgtcagggc
     2521 attagcaata cccttgatta tcaatagcat aaccataacc actgaaaatt aaaaatttct
     2581 atatatttca tattgttaaa tgatgcttaa ataaatttca tcagtaaatc attgccatag
     2641 ataatcattg gatggcacaa tcaattttcg ctaatgataa ccattcccat tacgttttgt
     2701 atgttgatag caaagtatct ctgtctgctg aagccctttt ggctgcgtaa gaataataaa
     2761 acttcagtgt tgttaatcat tattatcctc gcaatgatcc ttggcgtggt taaaattcag
     2821 gtctggttaa acgactggaa taatgatttt ttcaatgcgc taagccagaa agaaaccgat
     2881 aagctttggc agctcgttct ttggttccct gccctgctgg ggatctttgt attaatatcc
     2941 gcaaacaaaa catggttaat taaactactg actattcgct ggcgcgaatg gttaacagat
     3001 tactacctta atcgctggtt tgcagataaa aactactact tcacgcagat ctacggcgag
     3061 cataaaaata cagataaccc tgaccaacgt atcgctgaag acattctctt actgatcaac
     3121 aaaacgctca gtctttcatt tggcttcatc cagtcactta gcatgctgat cacctttacc
     3181 gttattcttt ggcaaagcgc gggtacgctc tcattcactg ttggtggaac agaatggaat
     3241 atccaggggt atatggtcta taccgtcgtg ctcattgtga tcggtggaac tttatttacc
     3301 cataaggtgg gtaaacgtat tcgtccgctt aatgtggaaa aacaacgtag cgaagcgaca
     3361 tttcgaacta atcttgtgca gcataataag caggcagaac tgattgcgct aagcaatgct
     3421 gagtctctac aacgccagga gttgcgcgac aattttcata ccattaaaga gaactggcat
     3481 cgtttaatga atcgtcagcg gtggcttgat tactggcaaa atatttattc gcgctcgctt
     3541 agcgttcttc cctactttct gttattgccg cagtttatta gcgggcagat caatctgggc
     3601 ggactgatga aatcgcgcca ggcatttatg ctagtatcga acaatttaag ctggtttatt
     3661 tataaatatg acgaacttgc tgaactggct gcggttatcg atcgcttgta tgagttccat
     3721 caactcactg aatagcgccc tacgaataag cctaaaaatt gccaacatgc ggtacaagtg
     3781 gctaatgcga gtattcgtac gcctgataat aagatcatat tagagaactt gaactttcat
     3841 gtttcgccag gcaaatggct attactgaaa ggctactctg gtgcgggaaa aaccacactg
     3901 cttaaaacat tatcccactg ctggccgtgg tttaaaggtg atatttcttc tcctgctgac
     3961 agttggtatg tgtcacaaac accgttaatc aaaagcggct tactgaaaga gattatttgt
     4021 aaagcacttc ccctgcccgt agacgataaa tcgttgagcg aagtactgca tcaggttggt
     4081 ctgggaaaat tggctgcgcg tattcatgac cacgatcgct ggggagatat tctttccagc
     4141 ggcgaaaaac aacgtatcgc cctggcacga ttaattttac gacgtccgaa atggatattt
     4201 cttgacgaaa ctacctctca tcttgaggaa caagaggcta tacgcttact gcgtttagtg
     4261 cgtgaaaaac tacccacaag cggcgtcatt atggttacac atcaacccgg tgtctggaac
     4321 ctggctgatg atatttgtga cattagcacg gttttataac aacaataaaa gccctgctgt
     4381 acggagatat aaataaatga agcgagttct tattcctggc gtcattttat gtggcgctga
     4441 tgtggcgcag gccgtcgatg acaaaaacat gtacatgtat ttttttgaag agatgacggt
     4501 ctatgctcct gtccctgtac ccgtaaacgg caacacgcat tacaccagtg aaagcatcga
     4561 gcgtttaccg accgggaatg gcaatatcag cgatctgctg agaaccaacc ctgcggtacg
     4621 catggattca acgcaaagta cctcgttgaa ccagggagat attcgccctg agaaaatctc
     4681 tattcacggt gcgtcgccct accagaatgc ctatttgatt gacggtatta gtgcaactaa
     4741 taacctgaac ccagcgaatg agtccgatgc cagtagtgca accaatatta gcgggatgtc
     4801 acaggggtat tatcttgatg tcagcttact ggacaatgtg acgctttatg acagttttgt
     4861 gccggttgaa tttggtcgct tcaatggcgg ggtaattgat gcaaagatca aacgcttcaa
     4921 cgctgatgat agcaaggtga aattgggtta tcgcactacg cgtttggact ggttaacatc
     4981 gcatatcgat gagaataaca agagcgcatt taatcaaggt tcttcaggaa gtacctattt
     5041 ctctccagat tttaaaaaga acttttatac cttgtcgttt aatcaggagc tggctgataa
     5101 ctttggcgtt accgccggtt tatcgcgccg ccagtctgat atcacccgcg cggattatgt
     5161 ttcgaatgac ggcattgtcg ccggtcgggc acagtataaa aacgttatcg atactgcatt
     5221 gagcaaattt acctggtttg ccagcgaccg ctttacccac gatttaacct taaaatatac
     5281 cggctccagc cgtgattata ataccagcac cttcccgcag tctgatcgcg aaatgggtaa
     5341 taaatcctat ggtctggcat gggatatgga tacgcaactc gcatgggcca aactacgtac
     5401 caccgttggt tgggatcata ttagtgatta tacccgtcac gatcatgaca tctggtacac
     5461 cgaactttca tgtacatatg gtgatattac agggcgttgt acccgtggcg gattaggaca
     5521 catttcccag gctgtagata attacacctt caaaacacgc ctggactggc aaaaattcgc
     5581 cgtgggtgat gtttcgcatc aaccctactt cggcgcggaa tacatctatt ccgatgcatg
     5641 gactgaacgc cataaccagt ctgaatccta tgtgattaat gctgcaggaa agaaaactaa
     5701 ccataccatt taccataaag gtaaaggcag cctgggaatt gacaactaca cgctgtatat
     5761 ggcggatcac attagctggc ggaatgtgtc gttaatgccc ggtgtgcgtt atgactatga
     5821 caactatctg tcaaaccaca atatctcccc gcgctttatg acggaatggg atatttttgc
     5881 tgatcaaacc tcaatgatta ccgccggtta taaccgttac tatggcggga atattcttga
     5941 tatgggatta cgtgatatcc gcaatagctg gacggaatcg gtatcaggta ataaaaccct
     6001 gacgcgttat cagaatttga aaacgcctta taacgatgaa ctggcaatgg gattgcagca
     6061 aaaaatcgat aagaacgtta ttgcacgcgc aagtgaagcg catgatcaaa tcagcaaaag
     6121 cagtcgtacc gacagcgcga ctaaaaccac cattactgaa tataacaacg atggcaaaac
     6181 caaaacgcat tcgtttaacc tcagttttga actggccgaa cccctgcata tccgccaggt
     6241 agatattaac ccgcaaattg tctttagcta tatcaagagc aagggcaact tgtcgttaaa
     6301 caatggttat gaggagagca ataccggtga taaccaggtg gtttataacg gtaatctggt
     6361 ctcttacgat agcgttccag tggcagattt taataaccca ttaaagatct ccttaaacat
     6421 ggatttcacg catcaaccga gcgggttagt gtgggcgaat acgctggcct ggcaagaagc
     6481 gcgtaaagct cgcattatcc ttggtaagac aaatgcgcaa tacatcagcg aatattcaga
     6541 ttacaagcag tatgttgacg aaaaactgga tagcagcctg acctgggaca cccgcttgtc
     6601 ctggacgcca caatttctga aacaacaaaa cctgacgatc agtgccgata ttctcaatgt
     6661 actggatagc aaaaccgctg ttgatacaac gaacaccggt gtggcgacct acgccagtgg
     6721 ccgtactttc tggcttgatg tcagcatgaa attttaatag ttcggatttt acttaaccgg
     6781 gtaaattcgc ccggttttcg catggagata attatgagaa acctctgttt cttactgacg
     6841 ttagtggcaa ctctgttgct ccccgggcgg ctgattgccg ccgccttacc gcaggatgaa
     6901 aagttaatta ccgggcaact ggacaatgac ttgcgatata tgatttatcc gcatgctcat
     6961 ccaaaggatc aggtaaattt atggctgcaa attcataccg gttcattg
//
LOCUS       DADLTY010000199         6961 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.201, whole genome shotgun sequence.
ACCESSION   DADLTY010000199 DADLTY010000000
VERSION     DADLTY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.201"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(364..945)
                     /locus_tag="JN334_003666"
     CDS             complement(364..945)
                     /locus_tag="JN334_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439667.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(992..4849)
                     /gene="sigA"
                     /locus_tag="JN334_003667"
     CDS             complement(992..4849)
                     /gene="sigA"
                     /locus_tag="JN334_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HAY7439668.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(5210..6247)
                     /locus_tag="JN334_003668"
     CDS             complement(5210..6247)
                     /locus_tag="JN334_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HAY7439669.1"
                     /translation="MHRGFCVAHEPSILLPSVGAQLSNPDNITDNADRISDEWKAFDR
                     AIESNHGVPEAAARLKEHLRDFRNTHAAAQLGVSAVAALPGDRLSAALMVKSHGTVSV
                     DGKVSDADLTYLEEVANSTGQEVDKSRLTSQACARTALITDVGIALATELETAGQKWS
                     LGFPPKFQRVDLFNYNVLVRNYDSSAFKGDRYHNTKNGINADIGASTDLDDNWTLGLV
                     AQNLISRSIETKEVNGITETFRIRPQVTAGVSWHNAMFTTAFDVDLTPASGFTSDSNR
                     QFAAIGTEFNAWKWAQLRAGYRQNLAGNDGSAFTAGVGISPFDVVHLDVAGLIGTDNT
                     YGAVAQFQFTF"
     gene            6199..6576
                     /locus_tag="JN334_003669"
                     /pseudo
     CDS             6199..6576
                     /locus_tag="JN334_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6615..>6961)
                     /locus_tag="JN334_003670"
     CDS             complement(6615..>6961)
                     /locus_tag="JN334_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY7439670.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1734 a         1567 c         1572 g         2088 t
ORIGIN      
        1 atcacctgct attaatcccc gagtcagtac ttcgcggcct gacgcgatca ccctactcag
       61 tacttcagag cccgcctccg gtacctgcct ggcgatcgcc cctgtgcaac aaaataaatc
      121 aaccgttatc aaaccacgat cgttcgtatg cgccgccgat gacgcgcgcc tgaaaaacgc
      181 gtcaatatga gttccccgga agcttacgat aatacgctta tgaacattac aacacgctca
      241 tgcggaacct ggatggggga aggaacggtg aagctgttag cgaatgaccg gcgtttatac
      301 aaacgccggt catgatgaat gatatttcag aatatacctc gggctaaaat accgggacag
      361 agatcaggcg tgattaatct gcagcatttt taataacaga agtactgagc ttactttacc
      421 tttgatgatg taatagttat ttccgtcggt actcattttt ccctgaaata atgtcacgga
      481 tgtttcttcc tgctgatcct gcaggcgcag tcgtaaggtc atatttttaa tgagctgaga
      541 tccgggtggc aaggggtatt tgctgtcaga tactgggcga ctgagtctga tgtggcgcgt
      601 atacagcccg ttttcgtaca gggtggcctc tgttatggtg tcagtgacca gcatgtggga
      661 gacaatgctg tattcttttt taaagcaccc accagaaacg gtgattgtaa tgataatggt
      721 cttctcgatc gagatgtaaa tacccgtttc gttatactgt ccgtatcgga accatgagaa
      781 ctcagttgca ttccagccat gctccagaat atgtgcattt ttttgcctga tagtttcttt
      841 ttttattcgc tcatgtttaa ggcataacag gcagataaat aatgtcagaa aaagaaagct
      901 aataatgaga gcaagagcga tatagtctgt cacagggtaa tacatattct ctccggtaga
      961 agaagggccg caaacgcggc ccgggctgtt atcagaaaga gtaacggaag ttggcgttga
     1021 tcgcgttatc cacgttgtat ttaccaaatg ccgatttctc aaactcaaga ccgaagcgaa
     1081 gattatcgcg aatttcggcg ttgagaccaa cattcatgag aatacgaccg tctttttcac
     1141 ctttgatacg tttctcaccg gacgcatcac gcagtacggt ttcaccgttg gcaaacaggt
     1201 caaactggta gccaaggccg gcgcgggctg tgactttcca gtccttaccg gagaaggatt
     1261 tacccacatc aacaccggta cgcccaatca gcggattaaa gtccttatcc ttcatggtca
     1321 cgctcattcc gcgatcctgc cagtcgaacc gtttacctga tacggcccca taaaccagtt
     1381 ctgcctgcgg ctcaatccag gagtcttctg ttacatgata gcggtagcct gcttcagcac
     1441 ccacatacaa ggaatgagaa ctgtagtctt ttgttccgag tccagcaaag gtcgccgagt
     1501 actcattatc atggtgaaca tacttactaa tcaggtcgat ataggcacca gagtcaaata
     1561 tagcggaagc atacagacct gccccgacgg acttcgtctt gccactgaat gcattactgc
     1621 tggcatgact gtcggtatac gtcatagtca gaccagtgaa aaggtcaagt ccatccagct
     1681 catgtttttt atccacacca atctgcacat gggtgtagtt gtcactgtat ccaccagaag
     1741 ctgaacctgc tccgctcatg atgcgggccc atgcaccggc ctcgccattg gtgtcacgca
     1801 gatcccccat tcgtttattc aggttgttga cttctgtcag gaaggctttg taattaccgg
     1861 acatatacga ttttgctgca ccggaagatg ctgtattttc agccacctta tatccggtca
     1921 gggtccacgt ggtatgttcc tgatcctctt tcgtgctgat cacaggagat atgttgctga
     1981 aacctatggc ctgagtactg gctttaaata tctctttatt gcttcctttt ggggctgtta
     2041 tcaggtcaac gtttaatcct gagttattat ttcccacttt ctgtatatcg accgacagac
     2101 tgttattgct tccggttaac tgatttttaa caatgagttt atccgattga tttacagact
     2161 ggctcagatc agcccgcata atcacagcag tattagtggc atccagtgtg tttactgtca
     2221 cagtatggaa acccgaattc tctatgttac taaacaacaa aagactgtcg cgggttttta
     2281 atgattgcag ggctgactgt ccgtttactc tccataaggt gttatacatg ctggctgagg
     2341 ctgcaggagc atcaatacct ccggtataag aagtatcaaa gccacccagc aaacctgtag
     2401 ccagcggtgt gtgtgtggtt ttgtccaggt tatcttcttt atactggtca acaccaaaca
     2461 gaatagatgc ttttttgtct gattttatat taccattgac ccaggcataa ttttgagcct
     2521 ttaacgtggc accatcttct gtcatattat atccatctga catatagatt gccgggctgt
     2581 atttattatt ttctgtaggg gtgcctgtta atgtcagggt tccggccagt tcaatagctt
     2641 tatctacata catttttccc tgggcggtaa cagtagctcc tttgttgata atcaggtctg
     2701 tattaattaa ggataatgca ccagtcatag taacagttga tttatcacca acagttattt
     2761 tgctgttttg agcactcaga ctgccgttga aactactttc gcctgctgaa tccccggagc
     2821 gaagctgttg tctgaatcca aaaccttcgc cagtaatgtt tcttcctgag aacatgtcaa
     2881 tgtatgcagt tttatttccc agaacaattt gagagttttt agcctctatg tgtcctttta
     2941 cattagcatc ccggcctata ctcagggttg cgttttctaa atttaatttc cggaaattaa
     3001 atttacgact ttcccagtcg ggctgggaaa aagaggctat ctgattgttg gacttatatt
     3061 ccgtattgta acgtttattt acagtgcttt cctggtcctt tatggcagca gaatagtctt
     3121 tttgacataa aaaaggaatt ggacagttgt tattgccttc tttaaatatg gcatgttctg
     3181 tcgcatgtcc ttgtaatgtt aactgtgcat tccggacaga gatatcgttt ttgatatcga
     3241 ctgagccatc aaagatcaga ttggcattat tgttatgttg ctgggtagta ttgataatat
     3301 gatttatgct tatattacct gatacattcc catggtacat atatgcatca gtattagtaa
     3361 gtgataatgt tgattgcttc acgtttgagt tcgttacggt tgttcccgca tctgttgctg
     3421 cgattttctg aaatgactgg tcatagccgt ttagatccag tgttccgcca tttctggtaa
     3481 aatagatttc gccattaccg ctttcgctta aagcgtcttt ggcatttatt tttaccgtac
     3541 ctttacctcc ggccatgtaa attttattga aggctttttc agcactaagg atgacagtcc
     3601 cattacctat tttaaggtta tttccctgtg ctatttttac atccagagta ccactaccta
     3661 ttttatgcag attatctccg gcaacgccct taacattcca ttcaacggtg gtattattat
     3721 caatatctat cccggcacct ttataagact tatcttttcc gtacacttta tatgtctggt
     3781 tcttatcgaa tataagcccg ccaatgccaa gatcggtatc ctgcttgagc gttaataccc
     3841 ctcctcctga gacagacagg tccttatctt ttataatcga tgataactct tgttttttac
     3901 catccagctg catagtgttt gtgaaattct tttttaaatt gtttaccaga atattattaa
     3961 ataacgagta gtttgtccag gctatattgg tactactgat aaaaccaact gtactggtaa
     4021 caccatggaa aacccatttt tgttgttctt tatcatatag ataagaacca gaaccactat
     4081 cgccaaaaat tggtgagttt ttaaaagggg ttctgttggt ataaatgtaa ctcttgtttt
     4141 tccagtccag attaaaaaga cttgcgctta acaagtcggg attatagttt tgtccaaaca
     4201 gatattgttt gccgtttaat atcgttgagg ttgttccggc acctgctctg aagccaatta
     4261 tttgttcctt accgttgtag tttacgccat atcttttcat cgcatcttcg ggagaaagtg
     4321 agaagtcggc actctccgta acccctgttg tttccacgac gaatttattc agacgatgaa
     4381 ccgaaaagtc gccagatgat actctgttac ttgctttata tgctgtctga tcccatgact
     4441 gtgttgttat tgcatgatgc tgtgtaccgt tatgagtcgc agtaacactg tacgcacctc
     4501 caattgatgt tgttgcacct ttgttggaaa cagcagaaaa atcaggtaca ggtagttctg
     4561 gaaaatgaaa tatctttcca tctttcatca ttaattgaac atttgttgca cccggtctga
     4621 actctcctct gttttgagca aggtcaagat aatcgcgggt ccatatatta tttatatcca
     4681 gcattgctgc aaatgctgtt tcgccataac ttccatatat tgaggataaa ataataccga
     4741 ggataacttt ctttcttgat gtaccgacac taactttccg ggtcagttca gaaacagcaa
     4801 ctaatccacc tgtaatatga ctatatttca gtgaataaat tttattcata cgtgaactcc
     4861 gatatttctg agttgtggtt atgggatagc atttaattac aacggggaac tatcagtcat
     4921 ggtacgtaac tgataagggt ataatgataa tcactgtcag ttatatttgt atgacagatg
     4981 taacaaaatg aaaaaaaaga taagcacaag tgattgattg gttagatatt gatcacttgg
     5041 tgtttgtatt ttgaattttg cgatcaaaat ttgatctttg ttgtttccgg tcaatccttt
     5101 acaggttttc tgtattatcc gggggagtta agtctgtcga tgtccgacag acttttgatg
     5161 atggaaacaa catgcagcga taatgtgcat gctgttttgc aggtggaact cagaaggtga
     5221 actggaactg tgcgaccgca ccgtaagtgt tgtccgtgcc gatcaggcct gcaacatcaa
     5281 ggtgaaccac atcaaacggt gagatcccca ccccggccgt gaatgcactg ccgtcgttac
     5341 cggccagatt ctgacggtaa ccggcgcgca gctgtgccca tttccaggca ttaaattctg
     5401 tgccaatggc ggcaaactga cggttgctgt cggaggtgaa accgctggcc ggggtcagat
     5461 ccacatcaaa tgcagtggtg aacatcgcgt tgtgccagga gacaccggcc gtcacctgcg
     5521 ggcggatcct gaaggtttcc gtgataccgt tcacttcttt tgtctcaata ctacgggaga
     5581 tcaggttctg tgcgaccagt cccagcgtcc agttgtcatc caggtccgta ctggcaccga
     5641 tgtcggcgtt gatgccattt ttcgtgttgt ggtagcggtc gcctttgaag gcgctgctgt
     5701 cataatttct gaccagcacg ttgtagttga acaggtcgac gcgctggaat ttggggggga
     5761 agcccagaga ccatttctgc ccggccgttt ccagttccgt agccagagca atacccacat
     5821 cagtgatcag tgccgtgcgg gcacaggcct gtgatgtcag gcggcttttg tccacctcct
     5881 gcccggtgct gtttgccacc tcttccaggt aagtgaggtc agcatcagag actttaccgt
     5941 cgacactgac ggtgccgtgt gatttaacca tcagtgcggc agagaggcga tcaccgggaa
     6001 gggccgccac agcagaaacg cccagctgtg cggcggcatg tgtgttacgg aagtcccgga
     6061 ggtgttcttt caggcgtgcg gcagcctcag gaacaccgtg gttactttcg atggcgcggt
     6121 cgaaggcctt ccactcatca ctgatccggt cggcgttgtc ggtgatatta tccggattgg
     6181 agagctgcgc gccgacggac ggaagcagga tgctgggctc atgcgcgacg caaaatccac
     6241 gatgtatata tcagtactaa aagcgcgacg gcggaagaag ctctgaaact aatcggtgaa
     6301 ctgtacgcca tagagcacga tatacgcggg ttaccggtgt ctgaacgcct ggcggtcagg
     6361 caaatgcaga gtagaccgct cctgacttcc ctgtataagc tgatgcagga gaaagaacac
     6421 acgttatcga agaaatgtcg tctgggagat gcgttccggt atatcaggaa gcactgggct
     6481 gcgttgtgca acttctgtga tgatggcctg gctgaggtag ataataatgc agcagaaaga
     6541 gcgcttcgtg cagtctgtct cgggaagaaa actttttcga ggatcactca ttccgtgtct
     6601 gactgcttca gggtttaatt ttgcttctgc tgactgaatg atgattgggc agcaagtgca
     6661 atatcccaca taaatccagt caactcacga gccaccgccg taatggctac ttgtgatttt
     6721 ttcccttttg ctgtcagagt tcggtaacga gaacacaatc gttgttgagc tttccatgca
     6781 atatctttgg aatattgtgt tacagagtcc ggtcggtaat atataagcca tgctccaact
     6841 tttgcaggtg tacgataaga ccaggcggct tcgtaaagca gacgtctcag ttcgctgttt
     6901 ccaacttttg ttattcctct gggacgaata ctgtttccac tggaatattc acctggcacc
     6961 a
//
LOCUS       DADLTY010000200         6911 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.202, whole genome shotgun sequence.
ACCESSION   DADLTY010000200 DADLTY010000000
VERSION     DADLTY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.202"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..695
                     /locus_tag="JN334_003671"
     CDS             <1..695
                     /locus_tag="JN334_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY7439671.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LKSELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1034..1804)
                     /locus_tag="JN334_003672"
     CDS             complement(1034..1804)
                     /locus_tag="JN334_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439672.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1849..2283)
                     /locus_tag="JN334_003673"
                     /pseudo
     CDS             complement(1849..2283)
                     /locus_tag="JN334_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2295..2516)
                     /locus_tag="JN334_003674"
     CDS             complement(2295..2516)
                     /locus_tag="JN334_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439673.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2517..3200)
                     /locus_tag="JN334_003675"
     CDS             complement(2517..3200)
                     /locus_tag="JN334_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAY7439674.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3264..3575)
                     /locus_tag="JN334_003676"
     CDS             complement(3264..3575)
                     /locus_tag="JN334_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439675.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3572..4543)
                     /locus_tag="JN334_003677"
     CDS             complement(3572..4543)
                     /locus_tag="JN334_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HAY7439676.1"
                     /translation="MRHGLMEAACERRIPMPNWCSNRMYFPGEPAQIAEIKRLASGAV
                     TPLYRRATNEGIQLFLAGSAGLLQITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTH
                     LQNGVLLDEQNCLMLHELWLQSGTGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNE
                     DVSVWWNRLCDNVVPEKTMPFDLLTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYG
                     VKWPCGYDLNISSQGENFIQVDFDTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFC
                     GWQLYERGELVNVLWGELEWSSPTDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4914..5873
                     /locus_tag="JN334_003678"
     CDS             4914..5873
                     /locus_tag="JN334_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY7439677.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5873..6268
                     /locus_tag="JN334_003679"
     CDS             5873..6268
                     /locus_tag="JN334_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY7439678.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6451..6831
                     /locus_tag="JN334_003680"
     CDS             6451..6831
                     /locus_tag="JN334_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAY7439679.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1888 a         1786 c         1675 g         1562 t
ORIGIN      
        1 ttgatgcaga agccatatgt gaagcggctt cacgaccatc aatgcgtttt gtccagccaa
       61 gaactgagac ccagcaagca ataagggcat tacaccgggt cagagaatcg ctcatcagag
      121 ataaggtgaa aacaaccaac cagatccacg gtttcctgct ggaatttgga atcagcctgc
      181 cgacagggga tgccgtaata aaacggttgt ctctcgtatt ggctgaacac gaaatccccg
      241 aatatctgag ccgccttttg gtgaggttgc atacccacta cctttacctt gttgagcaaa
      301 tagcagagct gaaatcagaa ttaagtcagt caatcaacgc tgatgatacc gctcagcgca
      361 ttatgacaat tcctggtgtt gggccgatta ctgccagctt actatcgtcg caacttggtg
      421 acgggaaaca gttttcatgc agcagagatt ttgcggcttc cacagggctc gttccccggc
      481 agtatagcac tggaggaaaa tcgactctgt tgggtatcag taagcgaggt gataaaaatt
      541 tacgccggtt actggttcag tgtgcccgct cattcatgat gcagcttgaa cgccagcatg
      601 gaaaacttgc tgagtgggtt cgggagcaac tcaacaagaa gcattcgaat gtggttgcct
      661 gcgctctggc aaataaactg gcccgaattg cctaggcaat cactactcag caaaaagaat
      721 atcaggcctg atatatagat gctgacaaaa ctcaattagc ttattgcaaa caataagata
      781 caagttaccg tctggttttg cgaagattga ttattgatga catgaacggc aaatcgacct
      841 gattaaaaac ctaacagggg aaaatggctc actgaagccg aggggatttt taggattatc
      901 aggtgcggaa ctcatcgagg cgcgggcatc ccttgcccac agagacgccg gatagattta
      961 agcaagccca ccattaatca aaaatcagtg ttgcaaaaac gggggtgacc atagattttc
     1021 cccgccatcg tcatcatttc cggatttttg ccagggcatc cgccatgctg cggatttcgg
     1081 cccgggcttc ccggacaact tcccgcagaa caccacggtc aaaccaccgg cggggcggtt
     1141 catccggtat acgcagcaca tcgcaggcca gcccactgga caggatatgc tcaatacaat
     1201 cgctgaccag atacatttcc accgccgaaa aacgtgcgcc ccggttcagg atggcatcga
     1261 gcaccacggg caggttttca tcgtcttcat cgagccagca ggggtgatcg ctttccttca
     1321 cgaccaggcg aaagcggcgc gtctggcggg tcagcaacgt ctgaccgctt gtggtcagcg
     1381 ccgcccggat acgccgcgca tcttctgccc cccggcaact gacactgcat actgcatcgc
     1441 gcaccgcatc aagaactgcc ctgatttcgt cttcggtcag ccattcaccg gcatacggga
     1501 aagacacctg cgttccgtcc gccggaagta ccggcagcga ctgccactga ctgccggaca
     1561 gaacaagttc cgctccggtt ccggtcagtt tctgaatggc ggaggccagg tgcggcggca
     1621 gcacgccatc gtggcggtta acgagtaacg tcgccagccg gggcgcatcc agccgcacgt
     1681 aaatgctgat ccagcccgga tagttcgggt tacattggtc aacatccgtc tcttccggcc
     1741 agccctgcgc cagcagcagg ttacgggcgt gacgacaaat cagagcattc agcgtttcat
     1801 tcataattca tgaactccct gtatcgccgc ccacaggcgg cgataacatc agtcgttttc
     1861 gtgtgtgttg tttttcagca ggctgcgggc ctgatcaacc atcgcctgtc ggcgggtaac
     1921 gtattccggg agggggaaac cgtgatcccg ggcagcccat tcacgtttcc acagtgcctg
     1981 ttcagcgatc tgagtcaggg tctccagcag ttcagcaggg acggtcacct ggcggttttc
     2041 gcgcacgttg ctgataatct ccatcgccgc ggcggaagaa ataccggact caatccgcta
     2101 gtcttccggg atgtcctcca ggtgcgccag gacggtcctg atttcatgat cggacaggga
     2161 aatatccatt ccgtcagcga gggcctgaat ctcttccggg gaccagataa ccagcatcag
     2221 cggcgtgtct cccgaatact ttgctgccag cgcccggcat agcgtttcgc atgttccgta
     2281 catactctct cccctcaatc tttgaagcag gaatccagtt ccagaatttc ggtatatgcc
     2341 tccatcaaga ggcggtttgc gcgaaacaac gtctgacgca ccagacgttc cggcagggtg
     2401 tcagcattaa agctgtaccg ccgctccatc tcattcatgg ttgtacgcag gtcgcacatg
     2461 gcattggcca cctccgccac ttcggcgcga agtttttcgt gtccggcata gttcatttac
     2521 gcagcctccg gcataaacca gtcatcattc gcggccccct gctgcatggc ccgctgtacg
     2581 gcagccagtc gttgctgtcc ggcacggtga tactgctcaa gcagctcgat accgatatac
     2641 cggcgtccgg actggagggc ggcgacgcag gttgaaccgc tgcctgcaaa cgggtccagc
     2701 actatcgcat tcgggtgtgt gaagctctca atcagcggtt gcaggctggt aacgggcttt
     2761 tccgtcgggt gatggcggtt gcccgaatat ttccagccca acacgtccgg cagcgggttt
     2821 tgtggcagac gtggacggcc ttttgccagg atgtaggcgc attcgtggcg atagcccaca
     2881 tatgcggcct tcgatgtgta gtttttggtg aagaccaggt gaccaacaac gctgaatccc
     2941 gcatttttcc aggcagacat aaagcgatcg acgcggttcc agccatagaa gctcaccatc
     3001 agcgcgtctt ttttgagtac gcggtacatt tcattacagg ccggttgcag ccattcatca
     3061 gttttatcac cagcaatggt acgcccctga cggtcacgaa aaccgacgag atatggcgga
     3121 tcggtgagga tgaaatcaat ggcgttatca ggaatacggg ccataacatc gatgcagtta
     3181 ccgaggacaa aacgggacat aacagatcct tctcttacag agggcacccc tgtggtgccc
     3241 gttccccgct gcctgtgggc tgttcatcgc ttccggtaac gggtcaggcc gaccgtggaa
     3301 taccgcagcc gtcgtctttt tgacagcaag gttatgccgc gaaagcggct tgaagggcgt
     3361 tcatcagaaa agggcgacgg gctggcgtac actgtcaccg acaccagccg ggagcggtaa
     3421 aagcgcccgt cccgtgcccg ccggacgcgg gacactgacc acaggcagcg tttatgccgc
     3481 gccgcgtcag ccggcgcagt aaaaagagga gcacggcgac atcctttgtc ggaaaagaaa
     3541 cgggcggttg cccgcccgtc tccgtcatac ttcatccgcc ataatgcgcc acattgtcga
     3601 ctatccacgc aggtccggtg acttccggct gctcatcgtc atctgtcggg gaagaccatt
     3661 caagttcccc ccacagcaca ttgacgagct ctccacgctc atacaactgc cagccacaga
     3721 aatcacagcc ctgttcggca taccagtgct ccagcgtgca gctgaaacgg cggcttaatt
     3781 ctgcaataac gtcgctttcc ggctgacacc acggagtgtc gaaatccacc tgaatgaagt
     3841 tttctccctg actgctgata ttcaggtcat atccacaagg ccacttcacg ccataccgtt
     3901 ccgtatacca gtgatatgca gaaggaacac cgttcagaac accaccatta aagccgttca
     3961 cttcaacatc caggcgggtc ggcagaaccg tcagcaggtc aaacggcatg gttttttccg
     4021 gcactacgtt gtcacacaga cggttccacc acaccgatac atcctcgtta ccccatatgt
     4081 cacaccagtc acctcttttt gcggtgaaat gcacagtgat ggtttccctg gcatcatccg
     4141 gtaatccttc ccaacgacgc tggccagtac cgctctgtaa ccagagttca tgcagcatca
     4201 ggcagttttg ttcatccagc aacacaccgt tctgcaggtg tgtcagccag cgggtgaacg
     4261 cgatattttc cgtcgataca gcaccacgtc cggcagccgt cactccgggg cactgttcag
     4321 agcggatatt ttcagtgatc tgcagaagtc cggcacttcc ggccagaaac agctgaatac
     4381 cttcatttgt ggcgcggcga taaagcggtg tgactgcacc gctggccagt cgtttaatct
     4441 cagcgatctg tgccggctca ccaggaaaat acatacgatt cgagcaccag tttggcatgg
     4501 gaattctcct ctcacatgcg gcctccatca ggccgtgccg cacctgtaac cgggcgaccg
     4561 catacgaatg caggtgcggc cccgaagccg cagcgcgtgg aggaggaatg acgaaacgtg
     4621 accgggtgag gaggccggcg acagcgacac acttgcatcg gatgcagccc ggttaacgtg
     4681 ccggcacggc ctggttaaat agtatccgtc tcagttcgtg gcgcaaaatg agaaaccaat
     4741 aagaactcaa aatgagccac aacaagaaca taaagagact ctaaaagagc aacaccgttt
     4801 gacatagact caaaatgagt cacaataaga aacaataaaa aactcaaaaa gatgcaccga
     4861 cataaaggat gctcaatttg agataaacac aaactcaaaa cgaggcaaaa agaatgctaa
     4921 aagtatcatg cgatgatggt tcaactaacg taaaactggc ctggcttgaa gatggcgagg
     4981 taagaaccag tctgtcagga aacagcttta aagaaggctg gaacccggga ctgtttaatg
     5041 ccggcaaagt ttacaattac gtcgtggatg aaaaaaaata cacctatgat ttgggaagca
     5101 ctgctgtcat aggtacaaca catgtcagtt atcaatacag caccaccaat ctgcttgcca
     5161 tacatcatgc actgctgaca tccggccttc agccacagga tgtagaactg acagtaacat
     5221 tacctgtgac cgaattcttt gataatgaca atcaaccaaa tgaagaaaga atagaaagaa
     5281 aaaaggcaaa tgttctccgt gagatatctc tgaataaagg ggaaacattt aaaattaaaa
     5341 aagttaatgt aatgcctgaa tctttgccag ctgcatttga atcactaaaa aaagataaag
     5401 taaacaaact tgaacgcagc ctgattatcg atcttggagg aaccactctg gattgtggtt
     5461 taattctcgg tgcatttgaa ggcatttctg aaatccgtgg ctattcagaa atcgggacat
     5521 cccgcatcac tcataccgtc atgaatgcgc tgacgaaagc ctccacacca tgtaattatt
     5581 ttattgctga tgagttaata aaaaacagac acgataatga atatctccag acattaataa
     5641 atgatgtagc tgaaataaaa aacatttctc atgtaataga cagagaagtg aaatctctgg
     5701 cagaaagcat caggcaggaa atttcaacat tctcaggaat gaaccgaatc tatctcactg
     5761 gcggaggagc cgagcttatt taccctcaca taaagcaata tttcccgaat ctgaaagtga
     5821 acaaggtgga tgaacctcag tttgcattgg tgaaagcaat ggtgcatgcg taatggaaag
     5881 cagtgatcca aaaaaaagaa agaaagttgt ggcatacctg cacccggcac tttatccgca
     5941 ggacaacctg acacaacaaa caatagactc tctcccagta caaatgcggg gagatttcta
     6001 cagacaatcc cttatttgcg gagcagctct ttattctgtt gccccccgac tgttaacgct
     6061 aatcagcgtt tttttcagtg agaaaatcac tgcagaaaat ctggtgaaac ttatagaaca
     6121 aacaacagga tacacatcca catcaattga tataagtgtg ttaaaaaaca tcatagaggc
     6181 atcttcagaa aacaaatcag agagcatcac ttcaaaagat gatttcgaag aacaaaccag
     6241 acgcaatctt tctatgttaa aaaaataata aaagaagagc aggttcagtc aattacctgc
     6301 tcttctttta tacatactaa aaaatcagat ttttacgaat attcagatta tcataaaaaa
     6361 tgcaacagac aggaatgcaa taattttgca ttatctcgtt gcttgtaatg caaatatgca
     6421 ttattgtcag tgtgtgacaa taacagagag atgacgatgg cgaccattaa cgcacgtatt
     6481 gatgatgata taaagaacca agcagatgaa gtgctgaagc tgatgaatat cagtcaaact
     6541 caggctattg cagcttttta tcagtatatt actgaacaaa agaaactgcc atttgtgatc
     6601 acctcaattg taaaaacgcc gcatgattta ttacgtgaaa gtacagacat gttagctgaa
     6661 gctcttgcag tgatttccaa tctacaggtg tggactgaac agcaggatgg gattggaaaa
     6721 gcaaagctaa tggagtatta ccgccgcctc gacgcactgt actgttgtgc aaaagagaaa
     6781 ataggtcttc tttcagataa ccgggatgct gaactaggct gtgtccctta atcatctgag
     6841 ttatagtaac tgtctgttta cacaacatat tgaactatgg ctcgctacga ccttcccgat
     6901 gaggcatgga c
//
LOCUS       DADLTY010000201         6901 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.203, whole genome shotgun sequence.
ACCESSION   DADLTY010000201 DADLTY010000000
VERSION     DADLTY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.203"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..1117
                     /gene="lhgO"
                     /locus_tag="JN334_003681"
     CDS             <1..1117
                     /gene="lhgO"
                     /locus_tag="JN334_003681"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HAY7439680.1"
                     /translation="AGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATS
                     QLEMERMRALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAA
                     MAKIFQARGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKML
                     GLEPGFIICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPN
                     AVLAFKREGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQ
                     KYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPI
                     GAHIVSKVQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            1140..2588
                     /gene="gabD"
                     /locus_tag="JN334_003682"
                     /pseudo
     CDS             1140..2588
                     /gene="gabD"
                     /locus_tag="JN334_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            2596..3804
                     /locus_tag="JN334_003683"
                     /pseudo
     CDS             2596..3804
                     /locus_tag="JN334_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
     gene            4042..5442
                     /gene="gabP"
                     /locus_tag="JN334_003684"
     CDS             4042..5442
                     /gene="gabP"
                     /locus_tag="JN334_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HAY7439681.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            5463..6125
                     /gene="csiR"
                     /locus_tag="JN334_003685"
     CDS             5463..6125
                     /gene="csiR"
                     /locus_tag="JN334_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HAY7439682.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(6126..6575)
                     /gene="kbp"
                     /locus_tag="JN334_003686"
     CDS             complement(6126..6575)
                     /gene="kbp"
                     /locus_tag="JN334_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HAY7439683.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(6659..6817)
                     /locus_tag="JN334_003687"
     CDS             complement(6659..6817)
                     /locus_tag="JN334_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HAY7439684.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         1553 a         1824 c         1952 g         1572 t
ORIGIN      
        1 tgccggggtc tattacacgc ccggcagcct gaaggcgcag ttttgcctgg cggggaaccg
       61 tgccaccaaa gccttttgcg atcaaaacgg cattcgctat gacaactgcg gcaagatgct
      121 ggtcgccacg tctcagctcg aaatggaacg gatgcgcgcc ttatgggaac gcacagcggc
      181 gaacggtatc gagcgcgagt ggataaacgc cgtggaactg cgcgagcgcg aaccgaatat
      241 caccgggctt ggtggcattt ttgtgccgtc cagcggcatt gtcagctacc gcgaagtgac
      301 ggcggcaatg gcaaaaatct tccaggccag aggcggcgag attatttata acgccgaagt
      361 cagcgccctc agtgagcata aaaacggcgt ggtgatacgc acccgtcagg gcggtgaata
      421 tgaagcatcg acgctgatta gctgttccgg gctgatggct gaccggctgg tgaaaatgct
      481 cggcctcgaa ccgggcttta ttatctgccc gttccgtggc gagtatttcc gccttgcgcc
      541 ggagcataac cagattgtta accacctgat ttaccccatt cccgaccccg cgatgccatt
      601 tttgggcgtg catctcaccc gcatgatcga cggcagcgtg accgtcgggc caaacgcggt
      661 gctggctttc aaacgcgaag gctatcgcaa gcgcgacttc tcatttagtg acacgctgga
      721 gattttgagc tcgtcgggga ttcgccgggt gctgcaaaac catctacgct caggactggg
      781 cgagatgaaa aactcgctgt gcaaaagcgg ctatctgcgg ctggtgcaaa agtattgtcc
      841 ccggctttcg ttaagcgatc tccagccctg gcccgccggt gtgcgggcgc aggcagtatc
      901 gccggacggc aagctgattg acgattttct gtttgtcacc accccacgca cgatccacac
      961 ctgcaatgcg ccctccccgg cagcgacatc agcaattcct attggtgcgc atattgtcag
     1021 caaggtacaa acgctgttgg caagccagag taaccccgga cgcacgctgc gagcggcacg
     1081 tagtgtggat gccttacacg ccgcatttaa tcaataacct ttgaaaacag gacgtaacaa
     1141 tgaaacttaa cgacagtaac ttattccgcc agcaggcgtt gattaacggg gaatggctgg
     1201 acgccaacaa tggtgaagtc atcgacgtca ccaatccggc gaacggcgac aagctgggta
     1261 gcgtgccgaa aatgggcgca gatgaaaccc gcgccgctat cgacgccgcc aaccgcgccc
     1321 tgcccgcctg gcgtgcgctc accgccaaag aacgcgccac cattctgcgc aactggttca
     1381 atttgatgat gcagcatcag gacgatttag cgcgcctgat gaccctcgaa cagggtaaac
     1441 cgctggctga agcgaaaggc gaaatcagct acgccgcctc cttcattgag tggtttgctg
     1501 aagaaggcaa acgtatttat ggcgacacca ttcctggtca tcaggccgat aagcgcctga
     1561 ttgttatcaa gcagccgatt ggcgtcaccg ccgccatcac gccgtggaac tttccggcgg
     1621 cgatgattac ccgtaaagcc ggtccggcgc tggcggcagg ctgcaccatg gtgctgaagc
     1681 ccgccagtca gacgccgttc tctgcgctgg cgctggcgga actggcgatt cgcgcgggca
     1741 ttccggctgg cgtgtttaac gtggtcaccg gttcggcagg cgcggtcggt aatgaactga
     1801 ccagcaaccc gctggtacgc aaactgtcgt ttaccggtta gaccgagatt ggccgccagt
     1861 taatggaaca gtgcgcgaaa gacatcaaaa aagtgtcgct ggagctgggc ggcaacgcgc
     1921 cgtttatcgt ctttgacgat gccgatctcg ataaagccgt ggaaggcgcg ctggcctcga
     1981 aattccgcaa cgccgggcaa acctgcgtct gcgccaaccg tttatacgtg caggacggcg
     2041 tgtatgaccg ctttgccgaa aaattacagc aggcggggag caaactgcac atcggcgacg
     2101 ggctggagaa aggcgtcacc atcgggccgc tgatcgatga aaaagcggta gcaaaagtgg
     2161 aagagcatat tgccgatgcg ctggagaaag gtgcgcgtgt ggtttgcggc ggtaaagcgc
     2221 acgaacgcgg cggcaacttc ttccagccaa ccattctggt ggacgttccg gccaacgcca
     2281 aagtgtcgaa agaagagacg ttcggccccc tcgccccact gttccgtttt aaagatgaag
     2341 ccgatgtgat tgcacaagcc aatgacaccg agtttggtct tgccgcctat ttctacgccc
     2401 gtgatttaag ccgcgtcttc cgcgtcggcg aggcgctgga gtacggcatc gtcggcatca
     2461 ataccggcat tatttccaat gaagtggccc cattcggcgg catcaaagcc tcgggtctgg
     2521 gtcgtgaagg ttcgaagtat ggcatcgaag attacttaga aatcaaatat atgtgcatcg
     2581 gtctttaact ggagaatgcg aaggaacagc aataaagagt taatgcagcg ccgcagtcag
     2641 gcgattcccc gtggcgttgg gcaaattcac ccgattttcg ctgaccgcgc ggaaaactgc
     2701 cgggtgtggg acgtttaagg ccgtgagtat cttgatttcg cgggcgggat tgcggtgctc
     2761 aataccgggc acctgcatcc gaaggtggtg gccgcggtgg aagcgcagtt gaaaaaactg
     2821 tcgcacacct gcttccaggt gctggcttac gagccgtatc tggagctgtg cgagattatg
     2881 aatcagaagg tgccgggcga tttcgccaag aaaacgctgc tggttacgac cggttccgaa
     2941 gcggtggaaa acgcggtaaa aatcgcccgc gccgccacca aacgtagcgg caccatcgct
     3001 tttagcggcg agtatcacgg gcgcacgcat tacacgctgg cgctgaccgg caaggtgaat
     3061 ccgtactctg cgggcatggg gctgatgccg ggtcatgttt atcgcgcgct ttatccttgc
     3121 ccgctgcacg gcataagcga ggatgacgct atcgccagca tccaccggat cttcaaaaat
     3181 gatgccgcgc cggaagatat cgccgccatc atgattgagc cggttcaggg cgaaggcggt
     3241 ttctacgccg cgtcgccagc ctttatgcag cgtttacgcg ctctgtgtga cgagcacggg
     3301 atcatgctga ttgccgatga agtgcagagc ggcgcggggc gtaccggcac gctgtttgcg
     3361 atggagcaga tgggcgtggc gccggatctt accacctttg cgaaatcgat cgcaggcggt
     3421 ttcccgctgg cgggcgtcac cgggcgcgcg gaagtgatga aggtgttcga gcaggaaaat
     3481 ctgctgcaaa aagccaacga tctggggcag aagctaaaag acggattgtt ggcgatagcc
     3541 gaaaaacacc cggagatcgg cgacgtacgc gggctgggat cgatgattgc catcgagctg
     3601 tttgaagatg gcgatcacag caaaccggac gcaaaactca ccgccgagat cgtggcacgc
     3661 gcccgcgata agggcctgat tcttctctcc tgcggcccgt attacaacgt gctgcgcatc
     3721 cttgtaccac tcaccattga agacgctcag atccgtcagg gtctggagat tatcggtcag
     3781 tgttttgatg aggcgaagca gtagcaccgt ccccatgcag gaggcgacgc tgcgcgtctt
     3841 gtccggccta cgagaatcca agtcggataa ggcgtttacg ccgcatccga caatctgtac
     3901 gtgaacagga ataaatctat gtcggccgga taaggcggag ccgctctccg gcaaaaagaa
     3961 tcaataacaa ttaaacgcgt gacccgacgc gggaaatgtc ggggcgctct cccaagtgac
     4021 acactttcga gaggattcag gatggggcaa tcatcgcaac cacacgagtt aggcggcggg
     4081 ctgaaatcac gccacgtcac catgttgtct attgccggtg ttatcggcgc aagtctgttt
     4141 gtcggttcca gcgtcgccat cgccgaagcg gacccggcgg tattactggc ctatctgttc
     4201 gccggattac tggtggttat gattatgcgg atgctggcgg aaatggcagt tgccacgccc
     4261 gataccggtt cgttttccac ctatgccgat aaagccattg gccgctgggc gggctatacc
     4321 atcggctggt tgtactggtg gttttgggta ctggttatcc cactggaagc caacatcgcc
     4381 gccatgatcc tgcactcgtg ggttccaggc attcccatct ggttattttc cctcgtcatt
     4441 accctcgcct taactggcag taatttatta agcgttaaaa actacggcga atttgagttc
     4501 tggctggcgc tgtgcaaagt catcgctatc ctggccttta ttttccttgg tgcagtcgca
     4561 attagcggtt tttaccctta tgccgaagtg agcgggatct caagattgtg ggatagcggc
     4621 ggctttatgc ccaacggttt cggtgcggta ttaagcgcga tgttgatcac catgttctcg
     4681 tttatgggcg cagaaattgt caccattgcc gccgcggaat ccgacatgcc ggaaaaacat
     4741 attgtccgcg ccaccaactc ggttatctgg cgtatttcta tcttctattt gtgctctatt
     4801 tttgtcgtcg tggcattaat accgtggaat atgccggggc tgaaagccgt tggatcttat
     4861 cgctcggttc tggaattgct caatattccc catgcgaaat taatcatgga ctgtgtgata
     4921 ttactttccg taaccagttg cctgaactcg gccctgtata ccgcctcaag gatgctctac
     4981 tccttaagcc gtcgcggtga tgctcccgcg gtaatgggta aaatcaaccg cagtaaaacc
     5041 ccgtacgtgg cggtgttact ctccaccggc gcagcattct taacggtggt ggtgaactat
     5101 tacgcgcctg cgaaggtatt taaatttctg atcgacagct ccggcgctat cgccctgctg
     5161 gtttatttag tcatcgccgt ttcacagttg cggatgcgca aaattctgcg agcagaagga
     5221 agcgaaattc gcttgcgaat gtggcttgat ccctggctca cctggctggt catcggcttt
     5281 attacctttg tgttggtagt gatgctattc cgtccggcgc aacagttaga agtgatctcc
     5341 accggcttat tagcgatagg gattatctgt accgtgccaa ttatggctcg ctggaaaaag
     5401 ctggtattgt ggcaaaaaac acccgttcat aatacgcgct gataatgatc aggagtcaca
     5461 ccatgaccat tacgtctctg gatggctatc gctggctgaa gaacgatatt attcgcggta
     5521 attttcaacc ggatgaaaaa ttacggatga gtttactgac atcgcgttat gcacttggcg
     5581 ttgggccgtt gcaggaagca ctttctcaac tggtggcgga acggctggtc acggtggtga
     5641 atcaaaaagg gtatcgggtg gcgtctatgt cagagcagga gctgctcgat attttcgacg
     5701 cccgcgccaa tatggaagcg atgctggtca gtctggcgat tgcccgcggt ggcgatgaat
     5761 gggaggcaga cgttctcgca aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca
     5821 gcgagaaaat gcttgatgag tgggatctgc gtcatcaggc gtttcataca gcaattgtgg
     5881 cgggctgtgg ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc
     5941 gttatcgatt tatctggctg cggcgaacgg tgctttcggt ggaaatgctg gaggataaac
     6001 acgatcagca ccagaccctg actgcggcgg tactgccgcg agatacagcg cgcgccagtg
     6061 agttaatgcg ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca
     6121 attaactact cttccggaat acgcaacact tgccccggat aaattttatc cgggcttttt
     6181 agcatcggtt tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt
     6241 ttggaaatgg cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagtggct
     6301 ggtgtcgccg ttttcacctg atcatcaaca ctggcaatac cggaaatatt ccccaccgca
     6361 acaaggattt tctccttcgc ctcctgactc aggccgtcac cagtgaccgt cgctttgccg
     6421 tcggcaattt gaatgttcac tttatcggca tcaggtatac cggttttgct cagatgctcc
     6481 tgcaccttct tcgcctgatc gtctttatcg tgctgacctg taaccgcatc ccagagtttt
     6541 tctccggcat ctttaacaaa attgaacaga cccatagcta cctcattcgt taacggaaac
     6601 actcagaaag tgtagcagag gaacgcggcg cgttaacgcc gcgggggtga gcacaggttt
     6661 aatcgcgggt ttgcacccag aacgcgtgaa tcaaaccggg gatataccca agcagtgtca
     6721 gcagaatatt aataatgaac gcccaaccga accctttacc gagcagcacg ccgatcggcg
     6781 gcagaatgat ggtgatgacg attctccaga aacccatatg tactccctat aagaaaatta
     6841 ctcattgttt aaaaagagat tttatctctt aagcgtagta attttagcgg agcctgccag
     6901 t
//
LOCUS       DADLTY010000202         6896 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.204, whole genome shotgun sequence.
ACCESSION   DADLTY010000202 DADLTY010000000
VERSION     DADLTY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.204"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..2004)
                     /gene="purL"
                     /locus_tag="JN334_003688"
                     /gene_synonym="purI"
     CDS             complement(<1..2004)
                     /gene="purL"
                     /locus_tag="JN334_003688"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAY7439685.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQ"
     gene            2263..3819
                     /gene="mltF"
                     /locus_tag="JN334_003689"
     CDS             2263..3819
                     /gene="mltF"
                     /locus_tag="JN334_003689"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HAY7439686.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(3816..4352)
                     /gene="tadA"
                     /locus_tag="JN334_003690"
     CDS             complement(3816..4352)
                     /gene="tadA"
                     /locus_tag="JN334_003690"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAY7439687.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(4377..5012)
                     /gene="yfhb"
                     /locus_tag="JN334_003691"
     CDS             complement(4377..5012)
                     /gene="yfhb"
                     /locus_tag="JN334_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HAY7439688.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            5221..6069
                     /locus_tag="JN334_003692"
     CDS             5221..6069
                     /locus_tag="JN334_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY7439689.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(6408..6896)
                     /locus_tag="JN334_003693"
                     /pseudo
     CDS             complement(6408..6896)
                     /locus_tag="JN334_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         1697 a         1836 c         1733 g         1630 t
ORIGIN      
        1 ctgatcgcgc gccaccatgc cggttacgct gcggtcaccg atggtcacca ggaaagtttt
       61 ctccgccacg gtcggcagat gcagtacgcg tttcaccgcg tctgcaatgg tgatcccttc
      121 acggaccagc gcatcgccct tcgctttcag cgtttgtaca tcgcgggtca tcttcggcgt
      181 tttaccaagc aggacgtcca gcggcagatc gatcggctga ttatcaaaat gacgatcgtg
      241 cagagaaaga tgcagttctt cggtcgcttc accaatcacc gcgtagggtg cgcgctcacg
      301 cttacacagt tcgtcaaaca gcggtaattg atcggcagca accgccagca cgtagcgttc
      361 ctgggattcg ttacaccaga tttccagcgg gctcatgccc ggttcgtcgc ttagaatatc
      421 gcgcagttca aatttaccgc cgcgtccgcc gtcgctcacc agctccggca tcgcgttaga
      481 aagaccgccc gcaccgacgt cgtggataaa cagaattggg ttagcatcac caagctgcca
      541 gcaacggtcg atcacttcct ggcaacgacg ctccatctcc gggttgtcgc gctgtacgga
      601 agcaaagtcg aggtcggcat cagactgacc agacgccatg gaagacgccg cgccgccgcc
      661 aagaccgatg ttcattgccg ggccgccgag aacgaccagc ttcgcaccga cgttgatctc
      721 gcctttttgt acgtgatcgg cgcgaatgtt gccgatcccg cccgccagca tgatcggttt
      781 gtgataaccg cgcagctctt cgccattgtg gctgttcact ttttcttcat aagtacggaa
      841 gtagccgttc agtgccggac gaccaaattc gttgttaaac gccgcaccgc ccagcgggcc
      901 ttcggtcatg atgtccagcg cggtaacaat gcgctcaggc ttaccgaaat cttcttccca
      961 cggctgttcg aagccaggaa ttcgcaggtt ggatacggag aaaccaacca aaccagcttt
     1021 cggcttcgcg ccgcgcccgg tggcaccttc atcgcggatt tcaccgccgg aaccggtcgc
     1081 cgcgcccggc cacggagaaa tcgccgtcgg gtggttgtga gtttcgactt tcatcaggat
     1141 gtgcgccggt tcctgatgga aattgtagcg gcccgtttcg tggtcagcaa agtagcggcc
     1201 cacttcagaa ccttccatta cggcggcgtt atctttataa gcagagagaa cgtgatctgg
     1261 cgtggtttcg aaagtatttt tgatcatcct gaacagcgat ttcggctgtt gttcaccgtc
     1321 gataatccag tcggcgttaa aaattttgtg gcggcagtgc tcagagttcg cctgagcgaa
     1381 catatacagc tcgatgtcgt tcgggttacg accaagcttt gtgaaagcat cctgcagata
     1441 gtcaatttca tcttccgcca gagccaagcc aagacgcagg ttagcgtcga tcagcgcctg
     1501 acggccctgc cccagcaaat caacgcttgt gaccggagtc ggttgatggt gggcaaacaa
     1561 ctgctctgca tcatctaaat caaaaaagac cgtttccatc atgcggtcgt gcagttcagc
     1621 ggtaacctgc tgccattgtt cattggtcag cgtaccggct tctatatagt aagcaacgcc
     1681 gcgctcaagg cggtttacct gttgtagccc gcagttatgg gcaatatcgg tcgctttcga
     1741 agaccaggga gagatggtgc caggacgcgg ggtcaccagc aggagtttgc cttgcggggc
     1801 gtggctggcg agtgccggac catatttcag caggcgttca agttgtgcgt gctcatcatc
     1861 gtttaacggc gcattgaggt cagcaaaatg gacatactcg gcgtaaatat tgtgaaccgg
     1921 gagcctggca gcctgaaaac gtgccagcag tttgttgatt cggaatgccg acagtgcagg
     1981 cgaaccacgc agaatttcca tcataagtct ctcgtcttct aagctttcgg tgtacccaag
     2041 gggggggaaa cgggcgtcat tataaagaat ctgatgcgct gacgaaaccg tttgcgtgga
     2101 aataaaatca ccatcgtgaa ttagcaacgc gtgccgccaa tggctgtaat aagttgccat
     2161 ctggcgcagg tttacgcaaa atgccgctca tttatgagta aacctttcac tattattacg
     2221 ttttttcaag ctgggacgcg cacgacacag agaattaact aattgaaaaa attaaagatt
     2281 aattatctgt tcatcggcat tctggcactg ctgctcgcgg tcgctctctg gccatccatt
     2341 ccctggtttg gtaaagccga caaccgtatc gccgccattc aagcgcgggg agagttgcgt
     2401 gtgagcacca ttcatactcc cctgacttat aacgaaatca acgggaaacc ttttggcctg
     2461 gattacgaac tggcgaaaca gtttgccgat tacctcggcg taaaactgaa agtgaccgtg
     2521 cggcagaata tcagccagct gtttgacgac cttgataatg gtaacgccga cctgctggcg
     2581 gcaggactgg tctataacag tgagcgggta aaaaattatc agcctggccc tacctattat
     2641 tccgtgtcac aacaactggt ttataaagtg ggtcagtatc gcccacgtac gctgggcaac
     2701 ctgacggcgg agcaactcac cgttgcaccg ggtcatgtgg tagttaacga tctccagacc
     2761 ctgaaagaaa caaaattccc ggaattaagc tggaaggtag acgacaaaaa aggctctgcg
     2821 gaattaatgg aagatgtcat cgaaggaaaa ctcgattaca ccattgctga ttctgtcgcc
     2881 atcagcctgt ttcagcgcgt tcacccggag ctcgccgtag cgctcgatat caccgatgaa
     2941 caaccggtga cttggtttag cccgttagat ggcgataata ccctttccgc cgccctgctc
     3001 gacttcttca acgaaatgaa tgaagacggt acgctggcac gcattgaaga gaaatacctg
     3061 gggcatggcg atgattttga ttacgtcgat acgcgcacat ttttacgcgc cgtcgatgcg
     3121 gtactgccgc agttaaagcc cctgtttgag aaatacgccg aagaaattga ctggcgtttg
     3181 ctggccgcta ttgcttatca ggaatcgcac tgggatgcac aggccacttc accgacgggt
     3241 gtgcgcggca tgatgatgtt aaccaaaaat accgcgcaaa gcctcggcat tacggatcgt
     3301 accgatgccg aacagagcat cagcggtggc gtgcgttatt tgcaggatat gatgagtaaa
     3361 gtgccggaaa gtgtgccgga gaacgaacgg atctggtttg ccctcgctgc gtacaatatg
     3421 ggctatgcgc atatgctgga tgcccgcgcc ctgacggcaa aaaccaaagg gaatcctgac
     3481 agttgggctg acgtaaaaca gcgtctgcct ttacttagcc agaaacccta ttacagcaag
     3541 ctgacttacg gctacgctcg tgggcatgaa gcctacgctt atgtcgaaaa tattcgtaag
     3601 tatcagatta gcctggtggg ttatctgcaa gagaaagaga agcaggctac agaagcggcg
     3661 atgcaactgg cgcaggatta tccggcggta tcgcctacgg agttgggcaa agagaaattt
     3721 ccttttctct cgtttctttc ccagtcgtca tcaaactatt tgacccattc tccctctctg
     3781 ctgttttcca ggaaagggag tgaagagaaa caaaattaat ccgtcgagga ttgcgctttt
     3841 ttctgcgctt taatttcctg gcggcgcatg cgaaagaagt cactgagcaa cgccgcgcac
     3901 tcatccgcca gtattccttc cgtaatttcc actcggtgat tcatacccgg atgatgcagc
     3961 acatccatta aagatcccgc agcgccagtt ttcgcgtcac gcgcaccaaa gaccacgcga
     4021 ccaatgcgac tgtggatcat cgctccggca cacattacac atggttcaag cgtgacatac
     4081 aacgtggcgt cgatcagacg ataattttgc atcaccagac caccctgccg cagggccatg
     4141 atttctgcat gtgcggtggg atcatggcga ccaatcgggc ggttccagcc ttcgccgatt
     4201 acccgattgt tatgcactaa taccgcgccg accggcactt cccgctcatc ccaggcacgt
     4261 ttcgccagcg tcagcgcgtg acgcatccaa tattcgtggc taaattcgac ttcagacaaa
     4321 aagaaaactc cggttataaa agcgcggcgc attatacacg gacgctatgc tttactctat
     4381 tccagttgct ggagttcacc gcgcggggtt actcgccaac gatgctgaca gaaataaagc
     4441 agcggattgt cctgattact gtcgctatag ccactgtaca gccgcagcgg cgtgccgatt
     4501 ttgcgctcca gttgtgcgac cttttcatgt cccagacaac gcatcgtcaa tacccaacca
     4561 ccatagccac gctgaatttg gctggcgata agattaaccc gcggcagcca gggcgtatcg
     4621 aaataaaccg cttcaaccag cggctgcgga gagccggtaa tcaaccagat atcagcatcg
     4681 gaacttaaca ggtaggtggt taatcgctcc tgaaccagcg gaaaggcggt aacattgtcg
     4741 cgaaaccagc gcacgaaatc ggcctgcaac gtctgtaaac gtgcttcgct gtgaccaaaa
     4801 gtgcaccccc acagaagcag actcatcggc cagcgtgccg cacgaccttt tatcaataac
     4861 gcaatggcta taatcggtaa caacggcagg acaagtaacg cattcagcgg ttggcgacgc
     4921 agtaaatagc gcagaaaact gccgaacata tcctgctgat gcaatgttcc atctaagtca
     4981 aaaaacacca cacgacgctc gtgagttgcc aaaccatact cctctggact gaaacatcct
     5041 gattaatttt cgctactaca tagcctaaca gatagatcat cacttttccg gcaacaatcg
     5101 accgcatcat ggctaactgg aatttttaat tcatggcaat tagcggcaat ggaatataaa
     5161 attcactcgc gtgtgtctca tattgcctgt tgtcgccact atttcatcgc gaggaaagat
     5221 atgaacggct tacttcgtat ccgtcagcgt taccaagggc ttgcccagag tgataaaaaa
     5281 ctggcggatt atctgctgct acaacctgat acggcgcgcc atttaagctc tcagcaactg
     5341 gccaacgaag ccggagtcag tcagtccagc gtcgtgaagt tcgcgcaaaa actcggctat
     5401 aaaggttttc cggcgcttaa gttggcgttg agtgaagcgc tggcaagcca gccggaatcg
     5461 ccctccgtgc ccattcataa ccaaatccgc ggtgatgatc cgttacggct ggtcggcgaa
     5521 aaactgatta aagaaaatac cgccgctatg tacgcaacgc taaacgttaa tagtgaagag
     5581 aaactgcatg aatgcgtaac aatgttgcgc tctgcgcggc ggataattct gaccggtatt
     5641 ggcgcttcgg gtctggtggc gcaaaacttt gcctggaagc tgatgaagat tggcttcaat
     5701 gctgccgcac tgcgcgatat gcatgcgcta ctcgcaacag tacaggcgtc gtcccctgac
     5761 gatctgttat tagccatttc ctacaccggt gtacgacgcg agttaaacct ggcggcagat
     5821 gagatgctgc gagtgggcgg aaaagtgctg gcgattaccg gctttactcc gaatgccctg
     5881 caacagcgtg cttctcattg cctgtatacc attgccgaag aacaagcgac aaacagtgct
     5941 tcaatctctg cttgtcacgc tcagggaatg ttaacggatt tgctgttcat tgcgctgatt
     6001 cagcaggatc tggaactggc accagaacgt attcgtcata gtgaagcgct ggtgaaaaaa
     6061 ctggtctgag taaagaatgc gcgtataatg cccgcccggt ttgtgttgtt ttgagagttt
     6121 ccttatggcg ctgttaatca ctaaaaaatg catcaattgt gatatgtgtg aaatcaaata
     6181 aaaacacatt tattttcata gtgatacagc aatgtgttat tgaatgtgtt gttggattta
     6241 tggtgcaaaa aaattttgca taagttcagc aacacaaaga acaccacttt ctttaacgtc
     6301 ctccgcgccc taaaggacgg ggctttacgg cgcaaaaggc ctgtgacata caacattaac
     6361 ttttcagtca accgtagcac gttcttgtat acgccgtgtc tgcggtttta tataaccctg
     6421 gctggaaacc tcttatacaa agtcgattcg ccaacatcat agatgatcgc caccttctgg
     6481 cgagaaactc ctgatgcaat tagtcgcccg gcctgcgccc attattctgg tgaaagtttg
     6541 ggacggcgac cgccaattcg tccctgtgcg cgagcagctt ccagtcctgc ttttgttcgt
     6601 tcaacaatca gttcacgttc catttcagcc agggcaccca tcacatggaa gaaaaagcgt
     6661 cccattggtg tgctggtatc aattgaatca gtcagactac gaaagttgat gcctcgttcg
     6721 cgcaacgcct ccaccagcac gacaagatgc cgcatactgc gccccagccg atccagcttc
     6781 cagacaacca gtgtgtcgcc tgccgataat gtcttgagca gttttttcag ccccggtctt
     6841 tcggactttg taccacttat cttgtcttca aaaatcagct cgcatcctgc acagtt
//
LOCUS       DADLTY010000203         6804 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.205, whole genome shotgun sequence.
ACCESSION   DADLTY010000203 DADLTY010000000
VERSION     DADLTY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.205"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            409..1239
                     /locus_tag="JN334_003694"
     CDS             409..1239
                     /locus_tag="JN334_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY7439690.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="JN334_003695"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="JN334_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HAY7439691.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="JN334_003696"
     CDS             complement(2368..3828)
                     /locus_tag="JN334_003696"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAY7439692.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="JN334_003697"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="JN334_003697"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAY7439693.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="JN334_003698"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="JN334_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HAY7439694.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
BASE COUNT         1632 a         1715 c         1691 g         1766 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gagtgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgtgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgacgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg cagccatgcc
     3901 ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt tgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 actaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc gtcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg cagg
//
LOCUS       DADLTY010000204         6795 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.206, whole genome shotgun sequence.
ACCESSION   DADLTY010000204 DADLTY010000000
VERSION     DADLTY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.206"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            240..2672
                     /locus_tag="JN334_003699"
     CDS             240..2672
                     /locus_tag="JN334_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAY7439695.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2703..4208
                     /locus_tag="JN334_003700"
     CDS             2703..4208
                     /locus_tag="JN334_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HAY7439696.1"
                     /translation="MLQKALFFMEYNMSISSVIKSLQDIMRKDAGVDGDAQRLGQLSW
                     LLFLKIFDAQEEALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPAL
                     KNLTAPIDKNPRGYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIY
                     EQILKDLQSAGNAGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKN
                     KYVKSVADHQTLQQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWD
                     EQLDVIVTNPPFGGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDG
                     TLFGEGVKTKIKKLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYE
                     HPYPAGVKNYSKTKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFN
                     LDIKNPHQAETVSHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..5869
                     /locus_tag="JN334_003701"
     CDS             4208..5869
                     /locus_tag="JN334_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAY7439697.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6090..6431
                     /gene="symE"
                     /locus_tag="JN334_003702"
     CDS             6090..6431
                     /gene="symE"
                     /locus_tag="JN334_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HAY7439698.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6478..>6795)
                     /locus_tag="JN334_003703"
     CDS             complement(6478..>6795)
                     /locus_tag="JN334_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439699.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         2003 a         1612 c         1554 g         1626 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
     5581 tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt
     5641 tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag
     5701 tttttacaat attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa
     5761 gataaattct attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa
     5821 aacccaactg catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc
     5881 tcacaaaacc aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt
     5941 cacgctaatt ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga
     6001 tgatgtatcc aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc
     6061 acaatcacta tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt
     6121 tagaagcaga agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt
     6181 acccggatta cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg
     6241 gttttgccac cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg
     6301 cccaacccgc cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc
     6361 tgtcggcgcg taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga
     6421 aagtcgcctg atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta
     6481 taccaacgcg ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg
     6541 aagttgtgcg aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt
     6601 tgtcacatct ttggcaatac ctttcggatc gaccagttca tgaaactgca tattaagcga
     6661 caatcgcagt cggctttttt gcggcacaac atcaacaaaa ttcgtttccg ctttaaacgc
     6721 aatatatagc ttcaatactt cctgcgtgat ccctgcgtcc aggcgcataa cttcatcgcg
     6781 aagatgatcg aataa
//
LOCUS       DADLTY010000205         6697 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.207, whole genome shotgun sequence.
ACCESSION   DADLTY010000205 DADLTY010000000
VERSION     DADLTY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.207"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="JN334_003704"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="JN334_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HAY7439700.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="JN334_003705"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="JN334_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HAY7439701.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="JN334_003706"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="JN334_003706"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HAY7439702.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="JN334_003707"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="JN334_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HAY7439703.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="JN334_003708"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="JN334_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HAY7439704.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="JN334_003709"
     CDS             4025..4615
                     /locus_tag="JN334_003709"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HAY7439705.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="JN334_003710"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="JN334_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HAY7439706.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRARFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="JN334_003711"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="JN334_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HAY7439707.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="JN334_003712"
     CDS             complement(6551..6658)
                     /locus_tag="JN334_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439708.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
BASE COUNT         1427 a         1805 c         1841 g         1624 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgcg aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc caggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatag
//
LOCUS       DADLTY010000206         6690 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.208, whole genome shotgun sequence.
ACCESSION   DADLTY010000206 DADLTY010000000
VERSION     DADLTY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.208"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..1407
                     /gene="sbcB"
                     /locus_tag="JN334_003713"
     CDS             <1..1407
                     /gene="sbcB"
                     /locus_tag="JN334_003713"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HAY7439709.1"
                     /translation="QPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGEPEVFYC
                     KPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYNNVRFDD
                     EVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGLPSFRLE
                     HLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQ
                     MKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDIDTLRERL
                     YTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLKILRENP
                     QVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFV
                     DKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLVQQYADD
                     KEKVALLKALWQYAEEIV"
     gene            complement(1450..1677)
                     /locus_tag="JN334_003714"
     CDS             complement(1450..1677)
                     /locus_tag="JN334_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HAY7439710.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1691..2749)
                     /gene="yeeE"
                     /locus_tag="JN334_003715"
     CDS             complement(1691..2749)
                     /gene="yeeE"
                     /locus_tag="JN334_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HAY7439711.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(2928..4286)
                     /gene="plaP"
                     /locus_tag="JN334_003716"
     CDS             complement(2928..4286)
                     /gene="plaP"
                     /locus_tag="JN334_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HAY7439712.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4276..4338)
                     /gene="yoeI"
                     /locus_tag="JN334_003717"
     CDS             complement(4276..4338)
                     /gene="yoeI"
                     /locus_tag="JN334_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HAY7439713.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(4499..5482)
                     /locus_tag="JN334_003718"
     CDS             complement(4499..5482)
                     /locus_tag="JN334_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7439714.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(5528..6352)
                     /locus_tag="JN334_003719"
     CDS             complement(5528..6352)
                     /locus_tag="JN334_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7439715.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6435..6689)
                     /gene="yoeB"
                     /locus_tag="JN334_003720"
     CDS             complement(6435..6689)
                     /gene="yoeB"
                     /locus_tag="JN334_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HAY7439716.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1796 a         1764 c         1571 g         1559 t
ORIGIN      
        1 caacctacct tcctctttca cgattacgaa acctttggca cgcaccccgc gttagatcgc
       61 cctgcacagt tcgcagccat tcgcaccgat gacgaattca atgtcatcgg cgaacccgaa
      121 gtcttttact gcaagcccgc ggatgactat ttaccccggc ctggagcagt attaattacc
      181 ggtattaccc cgcaggaggc acgggcgaaa ggagaaaacg aagccgcgtt tgccgcccgt
      241 attcactcgc tttttaccgt accgaagacc tgtattctgg gctacaacaa tgtgcgtttc
      301 gacgacgaag tcacacgcaa cgttttttat cgtaatttct acgatcctta cgcctggagc
      361 tggcagcatg ataactcgcg ctgggattta ctggatgtta tgcgtgcctg ttatgccctg
      421 cgcccggaag gaataaactg gcctgaaaat gatgacggtc taccgagctt tcgccttgag
      481 catttaacca aagcgaatgg tattgaacat agcaacgccc acgatgcgat ggctgatgtg
      541 tacgccacta ttgcgatggc gaaactggta aaaacgcgtc agccacgcct gtttgattat
      601 ctctttaccc atcgtaataa acacaaactg atggcgttga ttgatgttcc gcagatgaaa
      661 cccctggctc acgtttccgg aatgtttggg gcatggcgtg gcaataccag ctgggtggca
      721 ccgctggcgt ggcatcctga aaatcgcaat gccgtaatta tggcggattt ggcaggagac
      781 atttcgccat tactggagct ggatatcgac acattgcgcg agcgtttata taccgcaaaa
      841 gccgatcttg gcgataacgc cgccgttccg gttaagctgg tgcatatcaa taaatgtccg
      901 gtactggccc aggcgaatac gctacgcccg gaagatgccg accgactggg aattaatcgt
      961 cagcattgcc tcgataacct aaaaattctg cgtgaaaatc cgcaagtgcg tgaaaaagtg
     1021 gtagcgatat tcgcggaagc cgaaccgttt acaccttcag ataatgtgga tgcacagctt
     1081 tataatggct ttttcagtga cgccgatcgt gcagcaatga aaattgtgct ggaaaccgag
     1141 ccgcgtaatt taccggcact ggatatcact tttgttgata aacgaattga aaagttgttg
     1201 ttcaattatc gggcacgcaa cttcccgggg acgctggatt atgccgagca gcaacgctgg
     1261 ctggagcacc gccaccaggt cttcacgcca gagtttttgc agggttatgc tgatgaattg
     1321 cagatgctgg tacaacaata tgccgatgac aaagagaaag tggcgctgtt aaaagcactt
     1381 tggcagtacg cggaagagat cgtctaattt gaagccgccg ccgctggtac ggcggaggac
     1441 aatgttgagt caggcttttt gaacggtgat gctccacgct gcatcgccaa tctgctgata
     1501 atcggtgatg gcatgtcctt cttctgccgc ccactgtggg attgcttcag ttgcctgggt
     1561 gcaatcaaac tcaattacca actcatcgcc gctggccatt tctgccagtg ctgcttttgc
     1621 ttctatgagt ggaaacggac agacctgcgt caccacatcc agctttttaa tcgccatctt
     1681 tacaccttac ttaatttgcc gcagcagttg ccagtcgcgc cttacgctgc ggtcgaacat
     1741 acacaagcca ggacgcagtc cagactccga gaatcataaa tacaaggcca atccagccct
     1801 gccaggtcat cattgcagtc ataaccagtc cattaccgat agagcaacca cctgcaatac
     1861 tggcaccgaa gcccatcagt acaccaccta acccgctacg taatgttgtt tgtgcatcag
     1921 ctgcgcgaac gcggaactca cggctcgctt tggctgcaat aaatgcctcc acgaagatcc
     1981 ctaacaccag gaaaacgccc cagttaatgt atttcacgtc acccgcgacc agaaattgaa
     2041 gaatattggc cgttggggaa gtgactccta acccaaacat gcgtccggtt gcttcactca
     2101 ggggccaggc taaaagcgca atcaaaccga tgagtacagc agtgacaaag ggatgccagc
     2161 gtttttcaaa cagaatatga gcgatcccgg ttcggcgcgg cggtaaggtc gcgactttga
     2221 gttttggctt cttcaactct ttcatcacca cccagagcgt tatcaccagc aaaacggtaa
     2281 ccaacggcca cacagacaaa ttaaaagtct cagcaataga gttatgttca gtagtgtagt
     2341 gctgcaaggt ttgatttaaa ccactggcgt gtggagaacg catcaccgca ctcatcacca
     2401 tataagtgaa aagcgcgatc caactgccga tcaatccctc acccgcgcga taccaggtcc
     2461 cggtggcaca tccacccgcc agaacaattc ccagcccgaa aagataccca cctataacag
     2521 taccaagcca cgggaacgcc ccggcttcgt aagtcagtag gcccgcctga attaacgcaa
     2581 aaacccccac gctttgtgcc gaaattgcaa tcagcaaggc gtaaaacatg cgattatttt
     2641 tcgcgatata catatcgcga aaaccaccag tcaggcaaaa acgcccacgc tgcatgacaa
     2701 atcccagcag agcaccacaa attagcccgc ttaatatcat tgaaaacata aattaattaa
     2761 ccagatgaat gttaatgggg aaatttttca tgactggtgg aatgcagacc aataaccaaa
     2821 ttctctaaat tagaacaaat ggttattaat gaggatttcg ataaaaaaga ataaaaaaac
     2881 cggagccaaa tgttcattcg actccggttt attattagaa aagatgatta tgctacgtct
     2941 tcgtactgcg gaaccggatt acggaagctc ttggtgacgc aagccaggta aatcagaccg
     3001 atagcagccc agatcagacc cagaaccatt gagctttctt ccaggttaac ccacagcgca
     3061 ccaacggtca gcgcaccaca catcggcagg aacagatact ggaagtgatc tttcagcgtc
     3121 ttgttacgct tctcacggat ccagaactgc gagatgaccg acaggttaac aaaggtgaac
     3181 gccaccagcg caccaaagtt aatcagcgcc gttgccatta ccaggtcgaa gttgattgcc
     3241 agcagggcaa tcgcgccaac caggatgatg ttcatcgccg gagtacgcca tttcgggtgt
     3301 acataaccga agaagctttt cgggaacacg ccgtcacgac ccattacgta catcagacgc
     3361 gctacgcctg cgtgcgccgc cataccggac gccagtacgg taatggtgga gaagatcagc
     3421 gcgccaacct ggaacgcttt acccgcaacg tacagcatga tttcaggctg tgacgcatcc
     3481 ggatctttaa agcgagagat atccgggaag tacagctgca ggaagtaagt tgcaaagatg
     3541 aagatcatgc cgccaatcag cgcggtcagg aaaatcgcac gcgggatcac gcgctctgca
     3601 tctttggttt cttccgacag attgctgatg ccgtcaaagc cggtaaagga gaagcacagg
     3661 attgtcgccc cggtaatcat cgggataaca tgcgcatcgc cagaccagaa cggacgcgtg
     3721 ctcgccagcg taccagcgcc ttcaccttca aatacgccat aaacaaccat gcccagaatc
     3781 accgcgatca gcactacctg caacacgacg attacagtgt tgacgttcgc tacggatttc
     3841 agactacgca ggttaaaggc ggtcataaag gccaccagcg ccaccacaaa catccacgat
     3901 ggaatggaag gcaccagagc ttcaaaatag attttcgcca gcagaatgtt gatcatcggc
     3961 gcgaacagat agtcgagcag agaagaccaa cccaccataa agccgacagt cgggctaatg
     4021 gatttctggg cgtaagtgta tgcagagcca gcagaaggat agcggcgaac cagcttcccg
     4081 tagctcagag ccgtaaacag gatcgcaatc aatgcgaacg cataggctgt cggcacatga
     4141 ccatccgtga ggcctgaaac gataccaaat gtatcaaaca gcgtcatcgg ctgcatatag
     4201 gctagaccca tcattacaac cggaactaac gtaagcgttt tacgcaattc cacgcgagag
     4261 gtgtttggag taacgttatg cgacatggtt attctccttt acggtgataa ccgtcgcgta
     4321 agcaaaaaat tgccccattt ttttggattc ctcagcgaca acaactgtcg atttttagta
     4381 aatatctatc cggtacgaag cccggcctct tggtatgaat gattggtttg aagcaaaaaa
     4441 taaccgacgc taattaaacg tcggttttta atcatttttt gacagcggcg cattgtgcct
     4501 aaatgggggg gaaatgacaa gagaatgaga ggcttgtcat aataattttt ctttgaatgg
     4561 ctgattttcc gtcatcagat tttgcataaa caccggcgat cgcactattt gctaaaattt
     4621 catctcgcca ccagccgctg gcaagccctg cggtctgttc attccagcca atccagacag
     4681 gttcaaagag agtttgggcg accactttct tctcgattaa cgcgccacta tcgagaaaac
     4741 gttgcgccag ataccgtggt aaatagccgc agccaaggcc gctaatttgc agctccagtt
     4801 tggttttaaa atcgaaaacg gtaatggctt cttgttcgtc gagaagttgt gaggccgtag
     4861 aagcgcctgc ctgcgcagta tcccccacca caatcgcccg atatcgttta atgatacgac
     4921 ggtttaacgg ttcttcttcc tgggctaacg gatgatgagg cgcgaccgca aagacttgtt
     4981 caagatcgcc caaccgcgag aaaccaaact cactggaaga tggtggctca tgcatcgcgc
     5041 caacgataat atccgctcgc ccctgagtca gggcatccca ggaaccaccg agtacgccat
     5101 tgataaactt caggcgcgtc acgctatgat gttgataaaa agcttcaatg agaggcgcaa
     5161 gcagagaaaa aggaaacgtg tcgtccacgc caatcaccag ctcgttttcc cagccttcat
     5221 gcagtttgat cgcctgcttt tccagttctc gcacggtatg cagaacttcc cgccctttct
     5281 ctaataacat ttttccggtg cgggtgaatt tagcgcggtg tccgctacga tcaagcaatt
     5341 gaatatttag gtcgctttcc agcttgtgaa cggtgtaact cagagcggac ggcgtcttgt
     5401 agagtttggc tgacgccgcc gcaaaactgc cctctttttc tatggcatcg aggatcatca
     5461 acacatccag cagtggtttc atacacgccc ccttgcagtg catgcggcgt cctgctggcg
     5521 tgatcgttta ctccaacggc attaccagcg gatcgggata ctggtattca aatcccagtt
     5581 cattacaaat ccgactgcca tcaataatct tacctttgcc gctgtccaga ctatttctga
     5641 actgcggagg ttgcagcccc agtaaacggg ccatctgcgg atagaaaaca ttacgcgcag
     5701 ggtgagctgg cgcacatata ttatagatgt gtccgccttt aggtgcctgt aacaacagag
     5761 tgatagcgcc aataacatct tctaaatgga ctaaattaac accatgttca ccatcagggg
     5821 cggtttttcc ggcaaagaag cgtcccggat gacgtcccgg tccgaccagg cccgcaagac
     5881 gcagaatatc gaccgaagta ccgggtaaat tgtgcagcca gtcttcgagt tcttctaata
     5941 ctcgtccact gttggttacc ggattacgcg gggtagtttc tttaaccgtg ccttgcgcgt
     6001 cgccatagac agatgttgag ctggtaaaaa taattcgggg aatacgatgg gccagcgcgc
     6061 tatccactaa ctcttgtacc gcttgtaaat agaactcatc gccggggccg ctacgacgtg
     6121 ccggaagcgt aatgaccagc gcatcggcat ccataagggc atccagatcg tcagaatcgc
     6181 aaactaactc aggctccatg cgaagcagat agctatcaat gccactcatt cgggccgctt
     6241 cgacaccatc ttgtgtggtt ttactcccgg tgacttgcca gcctcgcgct gaaagtgaca
     6301 tcgccagcgg catacctaac caccctaacc cgacaattgc gacctttttc atccgttatc
     6361 tcctgataca ccttagatct ataaggctac gctagcgtat caaaactgac aattcattct
     6421 atgaatgaat ctgttcaata atgataacga catgctgcaa tgagcagtga atcatcagta
     6481 accgcgtata ccagacggtg ctcctctgta atgcgtcggg accagaaacc tgacaaatta
     6541 tgtttcaggg gttctggttt ccccttacct tcaaatggtg ttctgcgggt atctttgata
     6601 agttcattga tctttttaac aattcgctta tctgtttcct gccagtacag ataatcatcc
     6661 catgattcct cagaccagat tagtttcact
//
LOCUS       DADLTY010000207         6651 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.209, whole genome shotgun sequence.
ACCESSION   DADLTY010000207 DADLTY010000000
VERSION     DADLTY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.209"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            7..657
                     /locus_tag="JN334_003721"
     CDS             7..657
                     /locus_tag="JN334_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439717.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="JN334_003722"
     CDS             629..1063
                     /locus_tag="JN334_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7439718.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1836..2885)
                     /locus_tag="JN334_003723"
                     /pseudo
     CDS             complement(1836..2885)
                     /locus_tag="JN334_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            3336..4526
                     /locus_tag="JN334_003724"
     CDS             3336..4526
                     /locus_tag="JN334_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HAY7439719.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4916..6376)
                     /locus_tag="JN334_003725"
     CDS             complement(4916..6376)
                     /locus_tag="JN334_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HAY7439720.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6373..>6651)
                     /locus_tag="JN334_003726"
     CDS             complement(6373..>6651)
                     /locus_tag="JN334_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY7439721.1"
                     /translation="ITSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGY
                     PSLLVSILFLGGVQLIGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
BASE COUNT         2093 a         1210 c         1344 g         2004 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact
     1141 tctacaatga tttcaactta ttttatttaa ttgatattac gaaacttaac tcatacataa
     1201 acaatatgac tcaatactgt cctaaatagc tgcgcagaaa agtgctccac tttcgctcag
     1261 cccttgctgg gcgaagcatc aggaaggcgt tctcagctag gttgtgttat atctggcttg
     1321 agttttgttg cccatacgca acacattacc catctgtaaa cgacaagtgt ggcagaatta
     1381 ggtgcagcta tttagactat atcgacgcag taacgataat agatatcgaa actgtactag
     1441 atattagcta cttatggtta ccggggggag tcttttgagc actggtgtta tcaattggct
     1501 tcaaataatt cgccaataaa ttatattgcg atagctcatc accttccagg ttgtttatag
     1561 ccagtttatc agcgtcacca cctactgttt atgcttttat ttctttgatt aataagtata
     1621 tgatatttta attcatctgg tgtatttctt tctcatgtgt tggtcgactg aatatctgtt
     1681 cagtgaaggg gtaattttat gtttgcactt cgaacattcc cttcagttgc taggaaagaa
     1741 aaccgtaaca tttgcgcatt aattttaaca gtataatctg agcataaata tgcgctatat
     1801 atctataaaa aggcgcaatt aattgcgcct ttttatcatt ttatcaaaaa tggcccaccg
     1861 actgtcctcg atagccaatg gccaatttta tctgatacat atacacaaat acaggtatat
     1921 atgattgcgc agataggatt tggaatagtc acgctgaagc ctataattgc taaagccata
     1981 gggataaata atttggtgat atactccatt ccacatatgt taagagtatt tcttccagct
     2041 cttacaataa agtcatttcc acaaattatt tttgctatga aaatattaaa gattatcaag
     2101 cctaccgtgg tgataatagt attaatagct taaatataac tacttgatat tatgtctgca
     2161 ttgaatattg agataatctt aaaaagtaaa ggtttctggt taagtagttg atacgcactc
     2221 attatagcgg taattgagaa gacaatgaaa ccaattttag attttccaaa aaacctgtca
     2281 cgtgttaatt ctaaaaacat acatctaccg agaggcaata accaccagta tgccatagcg
     2341 gagtctatgt tccaaaacca ttgaggatct agtaatgggt tatgattgag cactgtttgt
     2401 gacaacatga acgataacag tgatatgatt aaaatcacta tattgttctt gactatttct
     2461 ctaaagattg catcaattgc tattataaca aaaaggcagt ttataaacca tattgttcct
     2521 acgaattggt tatttctaac accatatatt atatcataaa tgtggttgta tatataatca
     2581 atcgtctcac ctgagttaat tgtgtttatt attaatatag aaaatgcaaa agtgaaatat
     2641 ggaatcatta atctgtaaaa ctttgacttg atataatcga aaacagataa gtcatttttt
     2701 ttgatagtaa aaaaaccagc agcgaaaaag aatagtggga cgtgataact aaaaacaaaa
     2761 ggatacaatt tgccagcccc caacccaagg tgacctaaat atatagcaaa aataccgaga
     2821 aattttaaag catcaaccca atcatgtctt atttttctgt tgctgtcgat tttgttatta
     2881 tacatttaaa gtaggctctc cagttttgtg tgatttttaa acactcattg caaatatgaa
     2941 tgcctgaaag tttatgatta ttaatcaacg agttcaatat taatttatta tttttcaaag
     3001 tcgcactata taggcattta cttccgcagt accaccgata gtatcgatat caaatcagcc
     3061 tgcggccgcg ataactttcg taacattctg ccagcggctt tgttgaataa atcaggttca
     3121 agataatcgt ctctgcatca ggttagagtt tcaggcaata catcctcttt ctggcatacc
     3181 tgccactctc gcaccatggt catagccttt acgatctgca tactcaccag accagagccc
     3241 gctccctttt aggctgagga atccccagaa aagaagacag gcatagagtt ttgtgatcta
     3301 ctgattgcgc taacaaattc aatgagatca cctctatgcc tgcccgtaaa gtatgccaga
     3361 cttttttccg taatgcttta gcttccacgc atcagtatcg acaaaatgcc attattgatt
     3421 ctgctgctgc tttagtcggt ggcgcatctc tttctcttac cagcattggc cggcatttac
     3481 ccggtcctgc tcgcgtgaaa gataaaataa aacgcgttga ctgtctgttg ggtaataaac
     3541 gacttcataa tgatatccct ctgatattca aaaatattac atccatgatg acaaataaac
     3601 tgtcgtggtg tgttatcgct gttgactgga gcggttaccc ttttcaggaa tatcatgtcc
     3661 ttcgtgccag cctcctctgc gacggtcgct ctatccccct gatgagtcag gtcttcccgt
     3721 caaaaaaaaa taacgaggcg gtggaaatcg cttttctgga tgcgctttcc ggggccattt
     3781 ctccccggac tcgtgttgtt attgttactg atgctggttt tcaaagtgca tggttccgtc
     3841 atatcaaatc acagggatgg gattttattg gtcgtatcag gggggtagtg aagttcaggc
     3901 tggatagtga caaagataag tggctggaca taaaaatgtg cagggggagt tcagaggcaa
     3961 aatacctggg gacgggaacc ctggcccgga aaaaacgctc gcagtgtgaa ggacattttt
     4021 atctttataa acattcacct aaaggcagaa aaagcaggcg tgccagaggg aggccgggct
     4081 taccaacaac ggaaaaagaa caaaaagccg caggcagaga accatggtta atattcagca
     4141 atacagctga gtttaatgcc aaaaaaatca tgaaactgta cagtcgaaga atgcagatag
     4201 agcaaaactt tcgggatgaa aaaagtgaac gctttggttt tggccttcgg gcgagcagaa
     4261 gtcggagggg agagcgtttt ctggtattaa gtctactggt tacactggcc agtatcgtac
     4321 tctggctact tggttatcat tttgaaaata aaggatttca tttaaagtat caggctaata
     4381 gtctcaaaaa acgaagagta ttgtcttttc tgacgctggc tgaaaacgtc ctgagattta
     4441 accctgaact tctacgacga gcacaacctg aaaagatatt gtgccggttg gccagcactt
     4501 accgaagtat ggtgctggct tactgatgaa aattttctgg ggatccctca gcctttcggg
     4561 gaggaatgag ggctctgata ttcttttgcc gtagttcatc gtgaaaccgt cggatgtcat
     4621 ttggcctgag tgacccatgc ctcccaaaag cagagtcgct agtgttatgt aattaggcgg
     4681 ttaaacttca attctcagtg ttaagtttgg gtaaacgctt gtaaatggta ttgctaattt
     4741 tttaattgac taatattaga aacggttagt gtgattgtat atagatattt gagtgtaagg
     4801 tatttaaagg ggcattcagc ccctccgtct atttgtgatt gcatccattg atgtgtgtat
     4861 tattttgagc ttgaaaaagg gaggcgctct ttaataataa ataaatctat caaagctaaa
     4921 tattaaatgg aagccaccct tttttctctg atagttttat tatcaccaaa ccatttattt
     4981 cagctatcga atctggattc ggccaggaag gtaaactctt cacaattgga taaatcttct
     5041 ctatttgttc tttatttgct ggtttgcaat tacagattcc atagtaattc ataaatgcaa
     5101 ccatcctaaa atagttgcca ccatcccacc aaaaaaaaga ttttccaaaa gtgtctgcgc
     5161 tcatggagag catatttcct gagtcgtttg aaccatatat atatatatat tttccgttaa
     5221 gggaaatgcc ttttgtttga gatgtgtatg atatttcttt agctaaagag atatctttct
     5281 gtcttgcgag agtatcattc aaaaatagat ttttggatgt tgaaacgcca ttaaatataa
     5341 taaataaaaa aaacatgcag tttaggtatt ttatagagcg aaaattgctt aagcaagaaa
     5401 acaaaattac tgctactata ggcattaaaa caaacaggcg aggtggcgcc cctgagccaa
     5461 caacaacgat aaatataacc ggaagtatat aaatgaaaat gattgatgaa atcaaataaa
     5521 ttgatcttgt ttttaattta tatgttaggg taaacatcag aattaataaa ggaatcagta
     5581 accacttgta taggtttaaa ccagtgggag gattgttata gaagttatat atattatcta
     5641 tggctgattt aacaagccat ttaatatcgc ttgcattttg tatataattt gagatgtact
     5701 cgttcgattc aagcgaaaaa taatgcttga tagctttggt taatagcagg taaattaagg
     5761 tagataaagc tatgagggat agagataaac atgaactata aaaagattca cgaatatttt
     5821 tctcatttcg tattatcgaa tttatctgca acccaatgac gaatgcaata atatatgtta
     5881 cgaatgtttg gtagattgcc attgaaagaa tggacagtac tataccagaa aatatcacaa
     5941 tcctattttt ttgcgagtga aaaataattg ctgatatcgc tgccagtaga aaagcaattc
     6001 ccacagtatc agcttggtta agaaactcta attgatagga gatctgaggg aaggtaataa
     6061 atactaacat acctatcaat aattcataag attctagctt tagcgatctg cagattataa
     6121 atgctgaaat gatgataaaa gataaggcta ttaatggtgt tatataaagt gaaaaaggct
     6181 cagggagaaa gtaatgtcgc aaaaaagtgt gaaaccaccg cccaagcgtg attgtttgat
     6241 aaaaattatt tgtaaactcg ccatcaatat taagtgttcc atcgtacaac cacccccaat
     6301 aacaataaat tgatattgca aaacatgata tgaaggcaag gagatttgca tttttaaata
     6361 aattaatttt aatcatgggt tacctcgatg agattttttt atgatgtatt tgggtctatt
     6421 ttttacttca atatagattc tacctatata ttctccgaga acaccaatcc cgatcagttg
     6481 cactccaccc aagaaaagta ttgatacaag cagggaggga tacccgcgta ctgggttacc
     6541 aaagacaagg gtgtctataa tcatccatgc accatataaa aatgaaatgc ttgcaacaaa
     6601 caagcctata taagtccata cgcgaagagg aaaggttgaa aaacttgtga t
//
LOCUS       DADLTY010000208         6609 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.210, whole genome shotgun sequence.
ACCESSION   DADLTY010000208 DADLTY010000000
VERSION     DADLTY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.210"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..800)
                     /gene="mutY"
                     /locus_tag="JN334_003727"
     CDS             complement(<1..800)
                     /gene="mutY"
                     /locus_tag="JN334_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY7439722.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFAD"
     gene            961..1680
                     /gene="trmB"
                     /locus_tag="JN334_003728"
     CDS             961..1680
                     /gene="trmB"
                     /locus_tag="JN334_003728"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAY7439723.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            1680..2006
                     /locus_tag="JN334_003729"
     CDS             1680..2006
                     /locus_tag="JN334_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HAY7439724.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            2190..2909
                     /locus_tag="JN334_003730"
     CDS             2190..2909
                     /locus_tag="JN334_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HAY7439725.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            3085..4131
                     /gene="ansB"
                     /locus_tag="JN334_003731"
     CDS             3085..4131
                     /gene="ansB"
                     /locus_tag="JN334_003731"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HAY7439726.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGCDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            4248..5210
                     /locus_tag="JN334_003732"
     CDS             4248..5210
                     /locus_tag="JN334_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HAY7439727.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(5311..6570)
                     /locus_tag="JN334_003733"
     CDS             complement(5311..6570)
                     /locus_tag="JN334_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAY7439728.1"
                     /translation="MLVFIGVPISFSIGIATVASMLLMFPWDIAAITVSQRLANGLDN
                     FALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNVLANMMFGSISGSA
                     VAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVLIVFSLTAGGVSVA
                     SLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAFLDALPSLLLVFIV
                     MGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILESVVTTSIVLLLIGF
                     SVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIFMDMTPAVLIFTPI
                     FLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGVKIQHLIKPLLLFY
                     AALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         1869 a         1572 c         1699 g         1469 t
ORIGIN      
        1 tcgtcggcaa actgcgggaa acagtataaa ccgccccaca atccgctcgg cggacgctgc
       61 gccagcaata cttcatcttc gtgctgtaac aggagaaagt agccggtgcg ctccggcagc
      121 gtctgtttcg gttttttgcc cggataaagc gaccagctat tgttggtggc ggcaatacat
      181 ccgttttgta gcggacagag cgaacatttc ggcttcgaac gcgtgcaaat catcgcgccc
      241 aaatccatca tcgcctgatt aaaccgttcc acgccgaccg cgggcgtcac ctgctcgctc
      301 aaactccata gtttattctc gacctctttt ttcccaggcc agccgcttac agcatagcag
      361 cgcgccagca cgcgtttgac gttaccgtcg agaatcggaa agtgcttacc cagagaaagc
      421 gagagaatcg cgcctgcggt ggaacgcccg acgcccggta acgccgcgac ttcttcaaag
      481 gtttccggga atttaccacc gtgtaaggtc gccacttgtt gtgccgcttt atgcaggttg
      541 cgcgcgcggg cgtaatagcc aagcccggtc cacaagtgga gaacttcgtc gagcggcgca
      601 ttggcgagat cggtcaccgt cgggaagtgc gccataaagc gttcaaaata ggggataacg
      661 gtcgcaacct gagtttgttg caacatcact tctgagagcc atactttgta gggcgtcttg
      721 tcaatttgcc agggcagcgt tttccgcccg tatttatcgt accagtccag aacctgggct
      781 gaaaattgcg acgcttgcat ggtcaccgac ttcactgttg ttgggggcaa gattgcagca
      841 cagcgacggc agggtgtaaa ccggaacttt ccgcagccac ggccttatca tgcttgcatc
      901 cggcaactaa ctttggataa tgcccgtttt cagaacactt tcacaagcga ctaaatcttt
      961 atgaaaaacg acgtcatttc accggaattt gatgaaaacg gccgcccact gcgccgtatc
     1021 cgtagttttg tgcgccgcca ggggcgactg accaaaggcc aggaacatgc gctggaaaac
     1081 tactggccgg tgatgggcgt tgagttcagt gaagatatgc tggatttccc cgcgcttttt
     1141 ggccgtgaag cgccggtgac gcttgagatt ggttttggca tgggggcgtc gctggtggca
     1201 atggctaaag atcgccccga gcaggacttc ctcggcattg aagtgcattc accgggcgtt
     1261 ggtgcgtgcc tggcttctgc gcatgaagaa ggtttaagca acctgcgcgt gatgtgtcac
     1321 gatgcggttg aagtgctgca taaaatgatt cctgacaatt cattgcgcat ggtgcagctc
     1381 tttttccctg acccgtggca caaagcgcgc cataataaac gccgtatcgt tcaggtgccg
     1441 tttgccgaac tggtaaaaag caaactgcag ctggggggcg tattccatat ggcgaccgac
     1501 tgggaacctt atgcggaaca tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac
     1561 ctgtcagaga gcaatgatta cgtaccgcgt ccggcatcac gtccggtgac gaaatttgaa
     1621 caacgtggtc atcgtcttgg tcacggagta tgggacttaa tgttcgagag ggtgaaataa
     1681 tggcaaagaa ccgtagccgt cgtctgcgta aaaaaatgca catcgacgaa ttccaggaat
     1741 taggattttc ggtggcatgg cgattcccgg aaggtacatc ggaagaacag attgataaaa
     1801 tcgttgatga ctttattaac gaggttatcg aaccgaacaa actggccttt gacggcagcg
     1861 gttatctggc ctgggaaggt ctgatctgca tgcaggaaat cggcaaatgc accgaagaac
     1921 atcaggcgat tgtgcgtaag tggctggaag agtgcaaact ggatgaggta cgcaccagcg
     1981 aacttttcga cgtttggtgg gactaagaaa gcatacgggc gatgacaaat gcaaaactgc
     2041 ctgatgcgct acgcttatca ggcctacaga gatgcacgat cgagtaggcg ggataaggcg
     2101 tttacgccgc atccggcatg gaaaacgcgt actttgttat caatctgggg ccagcatatg
     2161 ctggcctgat ttgttcttga gggaagacta tgatgcgcaa aatgctgctg gcggcagcac
     2221 tttcagtgac ggcaatgacc gctcacgccg actaccagtg cagcgtcacg ccgcgtgacg
     2281 atgtgattgt cagcccgcaa accgtgcagg tgaagggcga aaacggcaat ctggtgatca
     2341 cgccagacgg caacgtgatg tataacggta agcaatattc cctgaatgcc gcccagcgcg
     2401 agcaggcgaa ggattatcag gctgaactac gtagcacgct gccgtggatt gatgaaggcg
     2461 cgaaaagccg cgtcgagaaa gcccgtattg cgctggataa aattatcgtt caggagatgg
     2521 gcgacagcag caaaatgcgc agccgtctga ccaaacttga tgcgcagctt aaagagcaga
     2581 tgaatcgcat tatcgaaacg cgcagcgatg gcctgacgtt ccactataaa gccattgatc
     2641 aggttcgcgc cgaaggccag caactagtga atcaggcaat gggcggaatt ttacaggaca
     2701 gcattaatga aatgggcgcg aaagcggtgc tgaaaagcgg cggtaaccca ttacagaatg
     2761 tgctgggaag cttgggcggt ctgcaatcct caatccaaac cgagtggaaa aagcaggaaa
     2821 aagatttcca gcagtttggc aaagatgttt gtagccgcgt tgtgactctg gaagatagcc
     2881 gcaaagccct ggtcgggaat ttaaaataat cctctatttt aagacggcat aatacttttt
     2941 tatgccgttt aattcttcgt tttgttacct gcctctaact ttgtagatct ccaaaatata
     3001 ttcacgttgt aaattgttta acgtcaaatt tcccatgcaa agctaaggga taatgcgtag
     3061 cgtccacgta actggaggaa tgaaatggag tttttcaaaa agacggcact tgccgcactg
     3121 gttatgggtt ttagtggtgc agcattggca ttacccaata tcaccatttt agcaaccggc
     3181 gggaccattg ccggtggtgg tgactccgca accaaatcta actacacagc gggtaaagtt
     3241 ggcgtagaaa atctggttaa tgcggtgccg caactaaaag acattgcgaa cgttaaaggc
     3301 gagcaggtag tgaatatcgg ctcccaggac atgaacgata atgtctggct gacactggcg
     3361 aaaaaaatta acgccgactg cgataaaacc gacggcttcg tcattaccca cggtaccgac
     3421 acgatggaag aaactgctta cttcctcgac ctgacggtga aatgcgacaa accggtggtg
     3481 atggtcggcg caatgcgccc gtccacgtcc atgagcgcag acggtccatt caacctgtat
     3541 aacgcggtag tgaccgcagc tgataaagcc tccgctaatc gtggcgtgct ggtggtgatg
     3601 aacgacaccg tactggacgg tcgcgatgtc accaaaacca acaccaccga cgtagcgacc
     3661 ttcaagtctg ttaactacgg tcctctggga tacattcaca acggtaagat tgattaccaa
     3721 cgtaccccgg cacgtaagca caccagcgat acgccattcg atgtctctaa gctgaatgag
     3781 ctgccgaaag tcggcatcgt ttataactac gctaacgcat ccgatcttcc ggctaaagca
     3841 ctggtagatg cgggctgtga tggcatcgtt agcgctggtg tgggtaatgg taacctgtat
     3901 aaatccgtgt tcgacaccct ggcaaccgcc gcgaaaaacg gcactgcagt agtgcgttct
     3961 tcccgcgtac cgacgggtgc taccactcag gatgctgaag tggatgatgc gaaatacggc
     4021 ttcgtcgcct ctggcacgct gaacccgcaa aaagcgcgcg tcctgctgca gctggctctg
     4081 acgcaaacca aagatccgca gcagatccag cagattttca atcagtacta atcacctcgt
     4141 cccggcatcg tgccggggct ttttcacttc agacttacgt ccattgccaa ttttcattac
     4201 cctaatgata atcaccggaa taaattattc cgcgcgaggg ttttcgggtg aaaaagcaat
     4261 ggattgttgg cacggcgctg cttatgttga tgactggtaa tgcctgggca gatggcgaac
     4321 cgccaactga aaatatctta aaagatcaat tcaaaaagca gtatcacggc attctcaagc
     4381 ttgatgccat cagcttaaaa aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg
     4441 aaggcgatgt ctcttccagt gacgatctct atacctgggt cggtcagttg gcagattacg
     4501 aactgctcga acagacctgg acgaaagata aaccggtaaa attctcggcg atgttaacca
     4561 gtaaaggaac gcccgcgtct ggctggtcgg tgaactttta ctcttttcag gcggcagcca
     4621 gcgatcgtgg gcgggtggtc gacgatatca aaacgaataa taaatatctg atcgtgaata
     4681 gcgaagattt caattatcgc tttagtcagc ttgaatctgc gttgaatacc cagaagaact
     4741 cgattcctgc gctggaaaag gaagtgaaag cgcttgataa gcaaatggtg gcggcgcaga
     4801 aagcagcgga tgcatattgg ggaaaagatg caaacggtaa gcaaatgacc cgcgaagaag
     4861 cctttaaaaa aattcaccaa cagcgtgatg agtttaataa acagaacgat agcgaggcgt
     4921 tcgcggttaa atatgacaaa gaggtttatc aaccggcgat tgcggcatgt cataaacaga
     4981 gtgaagagtg ttatgaagtg ccgattcagc agaaacgaga tttcgatatc aacgaacaac
     5041 ggcgacagac ttttctgcaa tcacaaaaac tcagccgtaa attgcaggat gactgggtaa
     5101 cgctcgaaaa agggcaatat ccgttaacga tgaaagtttc agaaataaac agtaaaaaag
     5161 tcgcgatttt gatgaaaatt gacgatatca accaagctaa tgaacgctag aaaaaagata
     5221 ccgaacaact gcggcgcaat ggggtgatta agtaactcat gttaagtgcc tttgccggat
     5281 aatataaaaa gttatccggc aaattatttt ttacattagc cctagtagct gtggaatgaa
     5341 caaggatatt tgcggaatgt aagtgatcat catcaacgct actagcaatg cagcataaaa
     5401 tagcagcaat ggtttgatta aatgttgaat cttcacaccc gagatagaac aaccaacaaa
     5461 tagcgcacta cccaccggcg gtgttaacag cccaatacaa aggttggcca ccatcataat
     5521 accgaaatgt actgggtcca ttcccagctc ttgtgcaatc ggcaagaaga tcggtgtaaa
     5581 gattagtaca gccggtgtca tgtccataaa gatgccgaca attaataaga cgatattaat
     5641 cagcagcaat atgatcagtg ggttatcaga aatccccatc aatgcgtcgc tgatcatata
     5701 aggaatatca gcattagtca ttgcccagga catgcccacg gaaaaaccga tcaacagtaa
     5761 tacaatcgaa gtggtaacaa ccgactccag aatcagtttg ggtaaatggc gccattttac
     5821 ttcccggtaa atcagcaccg agagaataaa tgtgtagaca acggcaattg ctgacgcttc
     5881 agttgcggta aaaatgccgc ccagaatgcc gcccatcaca atgaacacca gaagcaaact
     5941 tggtaaggca tccagaaacg ctttacaggc ctgagcaaag gttgctcttt ctgaaagcgg
     6001 gtagccacgg cgtttagcaa taatgccgca gactatcatt accgcaagac ccattaagat
     6061 ccctggcaaa tatcccgcca taaacagcga tgctacagaa acgccgccag ctgtcaggga
     6121 aaacacaatt aacacgtttg agggcggtat cagtaatcca gtaatacaag atgagacgtt
     6181 tacagcggta gaaaatgccg gatcataacc ttctttggtc tgaatgggat tcagtgtgcc
     6241 accaacggca gcagctgccg caacggctga accagaaatg gaaccaaaca tcatattcgc
     6301 caggacgttc acatgaccaa gcgaaccagg aacgcggcca accattacct gggcgaggtt
     6361 aatcaaacgt atcgcaatac cgccgctgtt catcagtgtt ccggcaaaga tgaaaaaggg
     6421 gattgccaac agggcaaagt tatccagtcc attagccagt cgttgcgaaa cagtgattgc
     6481 cgcgatatcc cagggaaaca tcaaaagcat tgaagctaca gtggcaatac cgattgagaa
     6541 cgaaatgggc acaccaataa agaccagcac aaaaaaacag ccaaacagtg taatcgcgat
     6601 ataactatc
//
LOCUS       DADLTY010000209         6528 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.211, whole genome shotgun sequence.
ACCESSION   DADLTY010000209 DADLTY010000000
VERSION     DADLTY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.211"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            163..648
                     /locus_tag="JN334_003734"
     CDS             163..648
                     /locus_tag="JN334_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439729.1"
                     /translation="MRAIYLSVQQAWNGKITYSVSGESEFAKKFQGKALPFDVRIISA
                     SQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSAGLEKVAKCINCNNTLQVNIPHEA
                     GHVLGYLDDDYDSSSPYVGDISGLMNVGMELWERYLKNATITLNIIMPETKFTLLNVT
                     K"
     gene            645..1031
                     /locus_tag="JN334_003735"
     CDS             645..1031
                     /locus_tag="JN334_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439730.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1056..3269
                     /gene="tssI"
                     /locus_tag="JN334_003736"
     CDS             1056..3269
                     /gene="tssI"
                     /locus_tag="JN334_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HAY7439731.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3292..3737
                     /locus_tag="JN334_003737"
                     /pseudo
     CDS             3292..3737
                     /locus_tag="JN334_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3759..4166
                     /locus_tag="JN334_003738"
     CDS             3759..4166
                     /locus_tag="JN334_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAY7439732.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4163..6528
                     /locus_tag="JN334_003739"
                     /pseudo
     CDS             4163..6528
                     /locus_tag="JN334_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108985.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         1639 a         1543 c         1887 g         1459 t
ORIGIN      
        1 acaaatattg atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat
       61 caaaatactg gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat
      121 accgttacag atgactggac tatatgggaa aaagctattt ttatgcgagc gatatattta
      181 tctgttcagc aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc
      241 gctaaaaaat ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag
      301 aatgaagact ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat
      361 gttgatttta aaaacagtac ggttcatgtt gattcagcgg gcctggaaaa agtagcgaaa
      421 tgtattaact gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt
      481 ggttacttag atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg
      541 atgaatgtgg gaatggagct ttgggaacga taccttaaaa acgcaactat aacgttgaat
      601 atcataatgc ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta
      661 ttatagtgat tttcctttca ataaattaca tagctatagt gaatgttttc aagtcttctg
      721 cagttgctca atccaccaaa tattatgaag aaaatgatat acatgatgga agtaatgaca
      781 tcttttttat gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca cgatataaat
      841 ttgttggtga gtattatatc ggcttgaatc aaacttatcg cataacagat cctgaatttc
      901 aagatgttcc tattaaggaa atgttctgga atttgcaggg ggatttgaat ctgacatgtt
      961 ggtttcatta taaagacggg caatggagag tgattactta tattttttgg ccaccagggg
     1021 ctagatttta atccggtatt aaaggagcaa atatcatgag ttttgtatct acgggtaata
     1081 aaccagttgg aaacggtgga cccgtcatca ccacaccacc aatagctggc gaaagtggcg
     1141 gtatgagcac aggttcagct gtaaccgatg tatctggtgc cgccgaggag atggcagaac
     1201 aggctgcggc ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag
     1261 tagctgctgc gcaggctgcc gctgcagcag cagggatctc ggatatggct ggtgcagttc
     1321 aggatgcagc ggcgtcgctt gcagccggtg ctccgggggc gcataacgtc acagtaagtg
     1381 gtagtgcggt gccgccgcag atgcttttgt ttgcaggcat gaatggttcg gaagggcttg
     1441 ggaatctgtt ttcttacaca gtacagctta aaacgctgga cgccctgaac ctggggtacg
     1501 tttcccccgc tgctaacctg ccgcttcagc tcatggtggg caaagacctg tgcgtcagta
     1561 tcgaactgga cggcggcgga aaacggtata tcagcgggct ggtgacggcg gcgagggtgg
     1621 tcggccatga gagccgttcg gtaacgtatg aattacgcat tgagccatgg ctgaagctgc
     1681 tgacccatac cagcgactac aaagcgttcc agaacaaaac cgtggtggat attctcgatg
     1741 aagtactgga tgaatattcc tttccggtgg aaaagcggct ggtggaaaat tatccacccc
     1801 gcgcctggca ggtgcagtat ggtgaaaccg acttcgattt catccagcgc ctgatgcagg
     1861 aatgggggat ttactggtgg tttgagcaca gcgagaacag ccacacgctg gtgctggtgg
     1921 atgccatcaa tggtcataaa gcgtgccctg actcgccgct ggtggagtgg caccaggaag
     1981 ggctgaaact ggataaggaa tttatccaca ccattacggc cagtgagagg ctgcgtactg
     2041 gaaagtgggt gatggacgat tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg
     2101 tggccagtcc gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg cctggtgatt
     2161 acttcactac gggcgaaggc gagatgctga cgcgcattcg tatggaagcg cagcgcagcc
     2221 caggcagccg ggcacatggg gccggtcata tccgtaccct catgaccggg tacaccttta
     2281 cgttgatgaa tcacccgacg gcagaaatta atcaggaata tctgctggta cagaccactc
     2341 tgtttttacg ggataatgcg cagcacagcg gtcaggatca gcattttacc tatgtcacca
     2401 cgtttgaact gcaccccacc agcgaagttt accgcccaca acgaaccctg agcaaacccc
     2461 acaccaaagg cccgcagagc gccatcgtca ccggcccggc ggggcaggaa atctggacgg
     2521 ataaatatgg ccgggtgaag gtgcagtttg gctgggaccg ttacggaaag aatgacgaaa
     2581 acagttcctg ctggatccgc gtcagctatc cgtgggcggg taaaggattc ggcatgatcc
     2641 agatcccgcg tattggccag gaagtactgg tggatttcaa aaacggcgat ccggatttgc
     2701 ccatcatcgt ggggcgcacc tataaccagg acaccatgcc gccgtggggg ctgccgggca
     2761 tggcgtcgca gagtgggata ttcagccact cattgcaggg agggccgact aacggaaata
     2821 tgctgcgctt cgatgataaa acgggtgcgg aagaggtgaa gttccatgcg gaaaaagacc
     2881 tcaataccac ggtaaaaaac aatgaaaccc acacggtcat ggtcgatcgc ataaaaacca
     2941 ttgtcaaaaa tgaaaccatc agcgtgggag aagaccgtaa tgccaccatc acaaaaaatg
     3001 atggtctttc cgtcaaactg gctcaaacca tcaatgtcgg aacgacatat cgtctggatg
     3061 ttggcgatca atttaccctt cgttgcggca atgcagcgct tgtattacac aaggatggct
     3121 cgattgagtt ttgcggaaaa caactgttac tgcacaccag cgatgttatg caactcattg
     3181 gcagtggcat tgatatgaat ccggatggcg gcacagccat gactgcggat gatattgccc
     3241 cgttacctac ccccgacgtt tctgaataaa aaatataaaa ggaatttttc tatgaccagc
     3301 ctttaccgta ttcaggaggg ctgctttgcc ctgcctgaaa cttttcttga tcgcaccgtc
     3361 aatatcttcg tcccatcggg caatgaaagg gcaactccca gtctgaatat tttccgcgat
     3421 acgctcaggc cagatgaaaa tctgaccacc tatattgacc gccagatagc cttgatgaaa
     3481 aaaaaaacct aggccagcac cgggtacttt cgcggggaac ggcactggcg ggaactggcg
     3541 aagctgccat gacaggtgaa cagattctcg caactcatcg ttctggaaaa acagaagtgt
     3601 accagcgtca ggccgggttt atcaccgggc cagcaaaggt actaatgttg acgctgacca
     3661 ctcagcgtcc ttttgatgat cacaccgacc agctatggac tgcatggttg actagttttc
     3721 agcctgctaa aagctaaaaa aaacagggag gttttgtcat gtatgaagcc gcacgggtgg
     3781 atgaccccat ctaccacacc agcgcccttg ccgggttttt gataggggcc atcataggta
     3841 tcgccattat tgccgtcgcc agctttgctt tttttacctg cggctttttg gccgggctga
     3901 ttttgagttt tctggctgat cagatagctt ccggagtcct gcaactgggt gaggctattg
     3961 ggcgctcgat acattccaca gtgggaaaaa ttctgaccgg ttctgataat gtcagcacca
     4021 acagtcgccc ggccgcccgg gccgtactga gtacggtgat atgcgaagac cattccccgg
     4081 aaatccgcat tgcccaaggt tccggcaaca tctacatcaa cagccagctg cccgtaagga
     4141 tgaccacact gagtgtgatg cagtgattga ggacggctcc cccaatgtct ttctcggcgg
     4201 cggtacgcag acggtactgg aaatcagccc ggaaatccct gactggctgc gtcaggtggt
     4261 ggatgtgctg tatgtggtcg ccggaatgct tggcgggctg gctggcgcct ggcgtcaggc
     4321 ggcaaagatg gggtcgaaat ttggtacaaa atgtgcggca aaatttattg gtggcgaaat
     4381 ggtcgggatg gccatctctg acacagtcat gggattgttc agcaatccgg tagatgtcac
     4441 cactgggcaa aaaattttgc tgccggagac ggattttacc ctgccaggac gcctgcctgt
     4501 cacctgttcg cgcttttacg ccagccacct ggaaactgaa ggattgctgg gcagaggatg
     4561 gcggctgaac tgggagataa acctgcgtga ggatgaaaca tatatcacct tcatcggtgt
     4621 acagggtcgg gaactgagtt atccgaaaga gatgctgata ccgggccacc agatattcga
     4681 cccggaagag cagttttacc tgagccgtct gcatgacgga tgctatgtgc tgcattacac
     4741 cgactgcagc tattacgtgt ttgatgaatt tgatgaccac ggcgtcgcgc cgttactttt
     4801 tatggagacg ccgtaccggc agcgtattgc tttcgggcgt gagaacggca ggctggtgcg
     4861 ggtggcctcc agcagcggac accatctgtt attgcaccgc acgatgacgc aagcagggga
     4921 acgcctgtcg catattgaac ttttgaaggg cgggcgtccc ggtaacctgg tggaatatcg
     4981 ttacgacgat aacgggcaac tgactggcgt ggtgaaccgg gctggtgtga cggtgcgcca
     5041 gtttgcctat gaaaatggcc tgatgacaga gcatcgcaac gcgacgggct ttacctgtac
     5101 ctaccactgg gaagagattg aaggtttccc gcgcgtggtg gaacacacga ccagcgacgg
     5161 cgaggattac cggttccact atgattttgc tggcgggcag acggtggtaa ccggcaggcc
     5221 ggagcagaag tggcagtggt ggtttgatga agagacgtat gtgactgcgc accggacgcc
     5281 gggcggcgga atgtaccgct tcacgtacaa cgaaaaccat tttccggtag cggtggaact
     5341 ccccggcgaa cgcagggtaa cgctggaata cgacaccctg agccgggtgg tgaaagagac
     5401 ggacccggca ggccgggtga cacagacgca gtggaacggc agttttgctg aaatcacgcg
     5461 ccgggcgctg gatgacgacc acgtatggaa ggcggattac aacgaacacg gccaggtgat
     5521 acgggagacg gacccggaag ggcgcgtcac gcggtacggg tatgatgatc aggggctgcc
     5581 ggagacggtc tgccaccccg gtaaacagca ggaccgttac acctggaacg cgctggggtt
     5641 actgagcagc caccgtcgca tcacgggcag tgtgcagtcc tggcagtata cgcagcgcgg
     5701 gatgctggcg cgccatacgg atgaagagaa acgggagacg cgctggcagt acaccccgga
     5761 agggctggtt gccagtctga gcaacggcaa cggggctcag taccggttca gctacgacgg
     5821 cgacggacgg ctgacgggag aacagcgccc ggacggcctg ataaggatgt ttgcgctgaa
     5881 tgcggacggg tttccggtca tcatcccgac gcagggcaca gagggcgggg tgcgcaatga
     5941 acagcaggag cgtgatgcgc tgggccgcct gctgcgcagt gacacgcagc acagtacacg
     6001 gaccttcagc tacaaccgtc ttgaccagat aacggaagtg acgctcaccc ccatggaaga
     6061 aggggaacgg ctgcaccaca tgcaggcgga cacggtgcgt tttgcgtatg accgcagcgg
     6121 gtggctgacg gcagagcaca gcgtgcacgg cagtatcaaa taccggcgcg atgcgctggg
     6181 caatcccacc gatatcacgt tgccggacgg gcagcatctc agtcatcttt actacggcag
     6241 cgggcatctg ctccagacgg cactggacgg tatcacggtc agtgaatatg agcgtgacag
     6301 cctgcaccgt caggtgatac gcacgcaggg gaaactggcg acgttcagcg gctacaacgc
     6361 cgacaaccgg ttaagctggc agcgcagcct gccaggtggc agccagaatc cacagcaggc
     6421 ggtactcccc cggcgcagaa cgacagcgtg acggcgcggg actatatctg gaacgcagac
     6481 ggtgaagtcg gcggcatcaa tgacaaactg cgcggttgcc tggtgttc
//
LOCUS       DADLTY010000210         6419 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.212, whole genome shotgun sequence.
ACCESSION   DADLTY010000210 DADLTY010000000
VERSION     DADLTY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.212"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..359)
                     /locus_tag="JN334_003740"
     CDS             complement(<1..359)
                     /locus_tag="JN334_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY7439733.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQR"
     gene            complement(645..1370)
                     /gene="phoU"
                     /locus_tag="JN334_003741"
     CDS             complement(645..1370)
                     /gene="phoU"
                     /locus_tag="JN334_003741"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAY7439734.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(1385..2158)
                     /gene="pstB"
                     /locus_tag="JN334_003742"
     CDS             complement(1385..2158)
                     /gene="pstB"
                     /locus_tag="JN334_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HAY7439735.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(2250..3140)
                     /gene="pstA"
                     /locus_tag="JN334_003743"
     CDS             complement(2250..3140)
                     /gene="pstA"
                     /locus_tag="JN334_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAY7439736.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(3140..4099)
                     /gene="pstC"
                     /locus_tag="JN334_003744"
     CDS             complement(3140..4099)
                     /gene="pstC"
                     /locus_tag="JN334_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HAY7439737.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(4186..5226)
                     /gene="pstS"
                     /locus_tag="JN334_003745"
     CDS             complement(4186..5226)
                     /gene="pstS"
                     /locus_tag="JN334_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAY7439738.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(5474..>6419)
                     /locus_tag="JN334_003746"
     CDS             complement(5474..>6419)
                     /locus_tag="JN334_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7439739.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         1636 a         1645 c         1506 g         1632 t
ORIGIN      
        1 cgctggatat cccacagcaa cacgttttgc tgaaagcgtt taatcgcaaa ctggcaatgg
       61 tcagttagcg agatataaat actgtcctgt aattttccca agcgctcctg cgctaaagag
      121 ataagacgat cacaggttgc catcacctca agaggaatat gacttaagag ttcgcttaat
      181 cgcccgttca gttcatgact gctcaaggca tactcttttt ctattccact tgagttaatt
      241 ctttcgccag cgcgtttttg aaagccaatt ccgcgcccca tgacgacttt ttcccgctgt
      301 tgatcatcaa taaccaccac aacattattg ttgagaattt tggtgatttg catgttcata
      361 gcaaggacct tttttataaa caaaaaaaac cgacttcacc agtattctct ggttatgtca
      421 ggttttgcct gcgaatgcag taacaatcca gtcatttatt aatggttttt ataacgaaca
      481 tccaggttcg gaaattaatt taattgcgtg cagagaaaat agcaatgcgc tattgataaa
      541 aatatgacca tgctcgcagt tattaacttt gtgtaatttt aggaatttat aaagttatat
      601 ataacaaatc ccaataatta agttattggg atttgtctgg tgaattattt gtcgctatct
      661 tttcccgcca gcagtttatc cagctcatca ccgcctacat gacggaaatc ctgacccttc
      721 acgtagtaga agataaactc acaaatattc tggcagcggt cgccgatacg ttcgattgaa
      781 cgcgcacaga acagcgcggt aagtacgctc ggaatagtgc gcgaatcttc catcatgtag
      841 gtcatcagtt gacgaacaat accttcgtat tcctgatcga cttttttatc ttcacgataa
      901 atacgtaccg cttcgtcaat gtccatccgc gcgaacgcgt ccagcacgtc gtgcagcatc
      961 tggatggtat gacggcccag cgactccaga cttaccagca acggctgatg ctgctgggag
     1021 aatttctcca gcgcagtacg gcagattttg tccgccacgt cgccaatacg ctccagctcg
     1081 gcaatggttt tactgatcac cataaccagt cgcaggtcgc tcgccgtcgg ctgacgtttg
     1141 gcgataatgc gcacgcaggc ttcatcgatc gccacttcca tcatgttgac gttcttgtcg
     1201 ccttcgatca cgcgcttcgc cagatcgctg tcctggttat gcatcgcggt gattgcatca
     1261 gaaagctgct gctccaccat gccgcccatg gtcatcacct gcgtgcggat actttccagt
     1321 tcggcgttga actggccgga aatatgttta ttaagattga gactgtccat aacgcactcc
     1381 tgaatcaacc gtaacgaccg gtgatgtagt cttctgtttg tttcttcgct ggcttggtga
     1441 acagatcgtc cgtgttgctg aactcaatca attcgcccag gtacataaat gccgtgtggt
     1501 cggaacaacg cgcagcctgc tgcatgttgt gggtgacgat caccacggtg taatcctgct
     1561 tcagttcggt gatcagctct tcaatacgcc cggtagagat ggggtcgagc gccgaacacg
     1621 gttcgtcgag cagcagcact tccgggcgaa tggcgatacc acgcgcaata cacagacgct
     1681 gttgctgacc accagagaga gagtaaccgc tctggtgcaa tttatctttg gtttcgttcc
     1741 acaatgcggc tttggtcaat gcccactgca cgcgctcgtc catgtcggca cgggagagct
     1801 tctcaaacag acgaacgcca aaggcgatgt tgtcgtagat ggacatcggg aacggcgtcg
     1861 gtttctggaa caccatgccc actttcgcac gcagcagggc gatatcctga gagttggtca
     1921 ggatgttgtc gccatcaagc agaatttcac cttccgcacg ctgctccggg tacagttcaa
     1981 acattttgtt gaaggtacgc agcagcgtcg atttaccgca gccggacggc ccgataaacg
     2041 ccgtaacctg gtttttggcg atatccaggt tgatgttttt cagggcatgg aatttgccgt
     2101 agtagaagtt caaattacga acctgaattt tactcggggc agtttcaacc atactcattg
     2161 caatcttttc ctcatcgtac ctgatgcttt tcgctaatca ggcttaccgt agggccgggt
     2221 aaggcgttca cgccgcatcc ggaaaagtgt taaccgtgtt tattcttcgc aaaaacaacg
     2281 cgcgccagaa tgttcagcag caggacgcac agggtaatga tcaatacccc ggcccaggcc
     2341 agttgttgcc attccgcaaa cgggctcatc gcaaacttaa agatcgtcac cggcaggttg
     2401 gcgatcggct gcatcatgtc cgtgctccag aactggttgg agagcgcggt aaacagcagc
     2461 ggcgcggttt caccggcaat acgggcaatc gccagcagga taccggtcat aatcccggac
     2521 accgacgctt tcagcgtaat cgcggagatc atcttccact tcggtgtacc cagcgcataa
     2581 gccgcttcac gcaggctgtc cggcaccagt ttcagcatgt tctcagtggt gcggataacg
     2641 atcggcacct gcaacagcgc cagcgcaatt acgcccgccc agccggagaa gtgctccatc
     2701 tgtgccacca cgatggtgta gacaaacaga ccaaccacaa tcgacggcgc ggagagcaga
     2761 atgtcgttaa tgaagcgaat cacttctgcc agccaggatt tacgaccata ttccgccaga
     2821 taaatccccg ccataatgcc cagcggcgta ccgaataccg tggcccacaa aattaacagc
     2881 ccgctacccg ccagggcgtt cgccagacca ccaccttccg tattgggcgg cggcgtcatt
     2941 tcagtgaaca gcgccagcga cataccgtcg ataccgcgag tgatggtgga cattaaaatc
     3001 cagatcagcc agaacaggcc gaaggccatc gtcgccatcg agagcgtcag cgcaatacgg
     3061 tttttgaggc ggcgacgcgc ctgcattttg cggcgagatt cagccagcgc cgcagtggtt
     3121 tgcatttcaa ccatagccat tagcgtgccc cctcattctt agccaggcgc ataatcataa
     3181 acttcgatgc ggcgaggacg atgaaggtaa tcacaaacag gatcaggccc agttccatca
     3241 gtgcggcaac gtgcagaccg gattccgctt ccgcaaattc gttcgccagc gcagaggtga
     3301 tactattgcc cggcatgtac agcgaggcgc tgtcgagctg gtaggtgtta ccgatgataa
     3361 aggtcaccgc catggtttca ccgagcgcgc ggcccagccc cagcatgatg ccgccgataa
     3421 caccattttt ggtgaacgga agaacgatac gccagataac ttcccaggtg gtgcagccaa
     3481 taccgtaggc cgactctttc atcatcaccg gggtttgttc gaacacatca cgcattaccg
     3541 ccgcaatgta cggaataatc atgatggcga ggatcacgcc tgccgcgagg ataccgatac
     3601 caaatgcggg gccagagaac agcgcgccaa caatcgggat attcgacatg atattgccga
     3661 ccggctcctg aaagtaaacg gcgaacagcg gcgcaaagat aaacaggccc cacatgccgt
     3721 aaacgatact tggaatggct gccagcagct caatggcgat acccagcggg cgtttcagcc
     3781 agccaggcgc aagctcagtc aggaacaggg cgataccgaa actcaccggg acggcgatca
     3841 gcagcgcgat aaacgaagtc accaacgtac cgtagatcgg caccagcgcc ccgtagatat
     3901 cgttcggtgc atcccactct ttggtccata ggaaagccag accaaatttc tgaatgctcg
     3961 gccaggagga gatgatcaga gagacaataa tgccacccaa catcaatagc acaatcagcg
     4021 ccgccagttt taccagcacg ctgaaaatta tgtcgccctt tttacccggt gggttaaaag
     4081 cgggcttggt tgcagccata agttactctt cagttaaacg cgtttacaaa gtcattttgt
     4141 aatgccggat gcggcgtaaa acaccgtacc cggcctggag ttttattagt acagcggctt
     4201 accgctactg tctttaatat tggtcttcca cgcagcgcga acctgttcaa ctacactatc
     4261 cggcaggctg gcgtaatcca ggtcgttcgc ctgtttagcc ccggttttgt acgcccagtc
     4321 gaagaatttc agcacctctg tgccttgttc tggtttcttc tgatctttgt ggatcagaat
     4381 gaacgtggta gaggtaatag gccatgcatc ttcgcctttc tggttggtca gatcctgagc
     4441 gaaggttttg ctccagtctg caccttttgc tgcattagcg aagttttctt cggtcggact
     4501 aaccggttta ccatcagcgg agatcagttt ggtgtacgcc aggttgttct gcttcgcgta
     4561 agcatattca acataaccaa ttgcacccgg cagacgctga acgaacgcgg cgataccgtc
     4621 gttaccttta ccgcccagac cgatcggcca ttttacggta gagccagtac caacgttgtt
     4681 tttccactct tcgttcactt tcgccaggta gctggtgaag acgaaggaag tcccggagcc
     4741 atctgcgcgg cgtactacag caatgttttg tgaaggcagt ttcagacccg gattcagttt
     4801 ggcgatggct tcatcatccc acttcttgat tttgcccagg tagatgtcgc cgagggtttt
     4861 accatccagc accagttcgc cagacttcag ccctggaatg ttaaccgcca gcaccacgcc
     4921 gccaatcacg gtcgggaact ggaacagacc ttcctgagcc agtttttcgt cagacagcgg
     4981 cgcgtcagag gcaccaaaat caacggtatt agcgataatc tgttttacgc caccggaaga
     5041 accgataccc tggtagttaa ctttattacc ggtttctttc tggtaagtgt cagcccattt
     5101 ggcatacacc ggcgcaggga aggttgcacc tgcacctgtc aggcttgctt ctgcaaacac
     5161 agagaaagca ctcatcgata aggtcgcggc gacaacagtt gcgacggtgg tacgcataac
     5221 tttcataatg tctcctggga ggattcataa agcattgttt gttggctacg agaagcaaaa
     5281 taggacaaac aggtgacagt tatatataag gaatatgaca tttttatgac agaaaggaat
     5341 taccaaataa cttactgatt ttaaatgtga agatgaaaat tccacatgcc atctttttgt
     5401 catgatgcat ttaaaagtga taaagaaatg ggtgagatga caacgatgtg atgaacaatc
     5461 gttgtcagac ctattagcgc acaatctcaa cagttatcgt cgctgttgca gtaaattgcc
     5521 ccggggctgg gcgagctcca gtaaggctcg caggagcagc tgaaaaagtt acactacctg
     5581 atttttcacc atacactgtt gatttatcca tgtctgttgg caactcacca ccgttcactt
     5641 tgatttcctt attatttttg tcgtaaattt ttatccccac gttagagttt gaagttacga
     5701 ccattgaatt gtccgtattg ggttcggtag cataaaaaga cgtactgata tcatcggtat
     5761 tcagtatccc caggttttca cactcaatag tgactgtttt agtaatttta tgtgttgtta
     5821 ccgcacttcc aacaacagat gaaaaatctg cagctggtat ttctttaaaa tcaaaacaaa
     5881 ctggctttaa attatttatt tcgcaacttt gcggagctgt aatatcgccc cgaatataaa
     5941 catcagccat cggctgtgag taatccaccg gggtgtcacg gctaattgtt ccatacaatg
     6001 tcactatctt tgtttcagga ataatcaatt ttcctgcaaa tgtcttcttg atataaaaat
     6061 atactatcgc tttagcccca ctggatatac ttaaaggttc aatgcggttg caattgtact
     6121 ttgtattacc agacgggttg ttagcatgat attcaaaagg tgcattcaca tacccaaccc
     6181 caagaatatc tatagcaatc ccaacatcaa ggttcggatt aagatcataa taattaatat
     6241 tagagtgaac cccagcataa gttaatgcct ggtttatttt tgacgtataa tagatatagg
     6301 ggaattctcc tacgttacca gtatcacaat gacagcgtcc aagatatgag gatccattag
     6361 ttacagtggt gttaaatgtc gttccaacct tattttcgtt cgcggtaata agatatccc
//
LOCUS       DADLTY010000211         6372 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.213, whole genome shotgun sequence.
ACCESSION   DADLTY010000211 DADLTY010000000
VERSION     DADLTY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.213"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(150..511)
                     /locus_tag="JN334_003747"
                     /pseudo
     CDS             complement(150..511)
                     /locus_tag="JN334_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(850..1404)
                     /locus_tag="JN334_003748"
                     /pseudo
     CDS             complement(850..1404)
                     /locus_tag="JN334_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1547..1669
                     /locus_tag="JN334_003749"
                     /pseudo
     CDS             1547..1669
                     /locus_tag="JN334_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1931..2497)
                     /locus_tag="JN334_003750"
     CDS             complement(1931..2497)
                     /locus_tag="JN334_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439740.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2779..3093)
                     /locus_tag="JN334_003751"
     CDS             complement(2779..3093)
                     /locus_tag="JN334_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439741.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3171..3281)
                     /locus_tag="JN334_003752"
                     /pseudo
     CDS             complement(3171..3281)
                     /locus_tag="JN334_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3605..4282)
                     /gene="ospD1"
                     /locus_tag="JN334_003753"
     CDS             complement(3605..4282)
                     /gene="ospD1"
                     /locus_tag="JN334_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspD1"
                     /protein_id="HAY7439742.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4654..4791)
                     /locus_tag="JN334_003754"
                     /pseudo
     CDS             complement(4654..4791)
                     /locus_tag="JN334_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4873..5171)
                     /locus_tag="JN334_003755"
                     /pseudo
     CDS             complement(4873..5171)
                     /locus_tag="JN334_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5760..5919)
                     /locus_tag="JN334_003756"
                     /pseudo
     CDS             complement(5760..5919)
                     /locus_tag="JN334_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5923..6372)
                     /locus_tag="JN334_003757"
                     /pseudo
     CDS             complement(5923..6372)
                     /locus_tag="JN334_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1706 a         1421 c         1298 g         1947 t
ORIGIN      
        1 ccggctactc attccctgct tgtgcaggat tttaatttcc ataactgtct caaaagtgac
       61 cataagctct cctgaatcag gagagcagat taccccctgg atctgatttc aggcgttggg
      121 tgtggatcac tattgcaccg ttcgtgacag tgttgttgcg ccttgctgcc aacgaagctc
      181 cactgcccat caagttcaca gataagggca acatcagcgt gagcgactgg cgcagatgtt
      241 atccgccggg gcgggagctt tttaatgtcc gtatgacggt attgatgccg attttcagcg
      301 tacggccagt atcgcgaact ccggcaccgt tgaaagccat ttcagtgatt tggtttttaa
      361 ctcccggttt acgggcctcg taacagtaag tgagctgaaa cacccggtga cagtcacggc
      421 aacggaatcg atcatgtcct tttgggttct gcccgtgacg gtaaacctga acggactggc
      481 aacgggggca atgaaagtta actgtgacca tgaaagaacc taaaagcggg tattataacc
      541 cctaattcaa ctaattggag gcattgtcat ttttaatcta tggatacact taaaaatcct
      601 ctcaccttac gttaaaaacc ttctcatgtt tagagttttt tattatgttt atcataatga
      661 tatgacgatc tgagttttta aaatcaagaa gaaaagataa gttttttaaa tgcacagtgc
      721 ctgtggataa ctttgcgtaa atccggagga cagatcactc tgaagcaaga caacagcatc
      781 tgaaaacaat atgctcacgt ttcaaccaca gggcgtaagt gatcctaccc acgtaatatg
      841 gacacaggcc taagcgaggt tctggttttc aaattgttcc ggactgaggc cgccacacca
      901 actgtgccgt cgccagcgat tgtaatcaca ttcgatataa ttaaacactg ttgccctcat
      961 tatttcccgg ctgataaagt gttctccgtg gagacattcc actttcagcg aatgaaagaa
     1021 gctttccacg caggcattat cgtagcagca gccctttgcg ctcatactgc cacgcagatt
     1081 atgccacttc agcagcgcct gataatctgc tgaacagtac tgacttcctc ggtccgagtg
     1141 aacgataacg ttccggggtc tcttacgccg ccacagcgcc atctgcaggg catcgcaggc
     1201 cagttgcgcc gtcaggcgtg gtgacatgga ccagccaata acggcacgtg accacaggtc
     1261 aatgaccaca gccagataca gccagccttc atctgtacgt aagtacgtga tgtctcccgc
     1321 ccacttctgg ttcggaccac tggcgtaaaa atcctgctcc agcagatttt ctgacacagg
     1381 caggccatgt gcgcggtaac tgaccgtatt gctgggtaag atcaatttgc tcactggtat
     1441 ttactacgtg catcgaatga gtgatcaaga gccgtatgac gcgagagtgt cacgtacggt
     1501 tccgtaagca gccggagagt gaaactctct ccggctgact cgactcgcag gttccgacag
     1561 cggtgacgaa catgcagcgg tgttgttctc gctgatcggc acatgccgtc tgaacaatgt
     1621 ggagccagaa aaatggctgc gttacgttat tgaacatatc caggactgac cggcaaaccg
     1681 ggtacgcgat ctgttgcctt ggaaagttga tctgacttct cagtaaatat caatatgatt
     1741 ctgacgagcc gcttaccttt gagcagagaa aggggagagg gatcccacat ccatgagcta
     1801 ggaaaataca aaaactggct atacaacaat tcaatatatt aggacttact tgcgaggaga
     1861 aatatagcac cgctcaggca attcctgggt atttactttt cttagtttct ttactcggga
     1921 tgttctgtca tcagaaaggc gattctaaat ttgtaatata gtcactcata ttcccacgta
     1981 atttctttag atcaatatat tgcacatttt tctcaaaaaa ctgatcggaa ataagatttg
     2041 caacattgct tgttgtatca tttttaacgc ttctattcat cccttcacaa agttcgtcca
     2101 ttttcttttt gaaataactg cttcctgata caagatgatt tatggaactt tgtgcagcat
     2161 tgatgtctag agaaatgcga taatctttct caacctgttt aaatatctgt ttacggagat
     2221 tatcgccgat ttttcggctg tgttctgcac aaaacttatc aatacattgg tttaccaccc
     2281 gggatacgta gacttttttt tcatcatttt tccaatctgt aatattttca ccgatttgtt
     2341 ctgaaacaga gctcaaaata ctagtattta ttttggttga tgatattttt actatccttt
     2401 tccccattgt acagcggata atctcatcca cctttcctga aaaatatctt ttgtgagata
     2461 aactgattcc aaaattatta aaagatgttc caagcattat aatcaccttg tcatgtaaaa
     2521 tagtaacatt gtcattattt tagctgattc aaacaattct gtaaatgctt tgagttcttt
     2581 actcccttgg ctcaatgatg gaccgtctgg aatcccagag actaaactct ctgagaaaga
     2641 gagtatgaaa aaacttgggg cagtccctaa atgaagggac caacagcggt aaaataaccg
     2701 gcatcattca taatgatgcc ctgttagttg tagttatgaa gatttacgtc attaaaattg
     2761 ctgttcgtag tgtcagtcct atggtctggc atcagctgag gatcgtagcc gatacatcac
     2821 tggcttcacg acattcgtgt tgaggctatt catgaaaact ccacgctgaa aacgccgttt
     2881 tgtataaacg gtcacggtat gcccggagcc actgttgctg ataaattcga taaaaccttg
     2941 gcgtttcttg aagctatcgt taatgcggat aatgaaacaa cgattggtga gattcgctct
     3001 tttgccgacg ccctggacgc tgtccggttc aatcgcaaca aaatcaaccg gcagctgagc
     3061 aaacccaacc ttgcctctct ggcgctggag catgaagtga tttggctggg acgtagccgc
     3121 tgacagcttc tataaaagta tgatcctacc cacgtaatat gaacacaggc ttaagtgagg
     3181 ttctggtttt caaattgttc aggactgaga cctccacacc aactgtgccg tcgtcagcga
     3241 ttgtaatcgc acttgatata attaaacact gtatatttca tttgtggagt ctccagtctg
     3301 agagggcttt atgcattccg tattgggtac tatgatgcca gaaatctgca aggctccaca
     3361 ccttcctgta tttcccttcc tctatcacag tgcactttac ttaatagggt gggatgtgtt
     3421 cattacattt attgtgtttt ttatgattgt ttttgcgtcc agtgtcgttc gtcatgggaa
     3481 actggttcta tgggtagtat cgctcagtct agttaactgt ttgtttcttt ttagaaatta
     3541 agcagatcac acaattcggg gagagatcct tctaagtgca attctttgca gctatttagc
     3601 acacttaaaa caactcttgt attttgtcag aaataatatt aaacatgtct tgttcattat
     3661 tgttaagtct gtctgctatt tctgtaagtt ttatcccact atgatggcat atatttatat
     3721 atgccatcat agcctcgata ttccctttcc ctgcggccag aaataatcct ggtaacccct
     3781 cattatctct tacatccaat agtttaatcg tttcttttgg tgataaatca aaagttttaa
     3841 taaaatctcc ataagcttga atcgcagcgg aatgaccttc ttgaagagct atgaacaaag
     3901 cagattcccc agcattattt cgtgctgcaa gaacttctag tttttcctct ttgggtattt
     3961 cggctgccaa cagcgattca ccgtatttac gcgctgcatc ggcatttcca tgccaaatca
     4021 atgaaaacaa attatggcgg ttatactcct tattattctt tgtcggaatg gatatgttct
     4081 gtattttggc caattttctg aaagaaacac cactatatcc cccccctgat ttttcttcat
     4141 tgatggcgtg ggatatagcg gtatttgtca cgttaatgat attatttttc attcctttat
     4201 tttcattagc agtattgctg cctattatta agtgcatatt tttgttgttt gctggatgta
     4261 atccatagtt atttattgac atttttgttc atcctttttg ctaaaaaaaa acaataacct
     4321 atttttgtaa tgtaatagat attagtctat tgctaatcta aaaaaaccat tgccacattg
     4381 aatggctata ttcaccaata aattctttcg atttcgtata ttgacatatc gtagatatat
     4441 cttatcccta agattcaata atcgagtccg tttaccattc tgtgcaagcg ccttcactca
     4501 gaagtgaccg tcaaggcgat caccaaactt gataataaag cagctcattg ccatacgcca
     4561 gtccttaagg ggcgttgtcc atttctgtga tgctgcctag atttaactgc cagctccggc
     4621 ttatctacat ctaaatatgt gagcctaata ccgctacact ttgtccagac tatgtttttc
     4681 tccgggaagt gggctaaccg tctccataaa gtgacgaact tttttcaaca gtggccatat
     4741 tgaacggcac tgatgattac gggttattgt ctcatgaaga gccggccaca tcactgacag
     4801 cagtgacttc aggccttcag caccagacag cctgaaccag ttaatccatc gcccgacaaa
     4861 tttagtaatc tagggctggt ttttcttcag ctgttgcggt tcgaaagtgc cattacgatc
     4921 acgcagagta cggatttcca gaggttcatc tccagtagtg ataatcttcg ttaagcaacc
     4981 attgcgggaa ttgctgccag atctaagctg atttttctcg cacccaagat gatgagtcat
     5041 attagcattg agtacggttt cattttcaac atccaatcaa actgactgag atcttcagga
     5101 tttttgagac ttttggctag ttcattagct aaagcccgca acttattaga tgtcaactgt
     5161 ttttcattca taaattcacc ttcatttgat atcaaattaa atagttcaac atcagagaat
     5221 tatacaaact tatgtacagg ctcctatgat cttcgaatcc tgaagattat agtgaaaata
     5281 agttttcacg ttgaaatccc acatagttag tatatgataa ataatcatgg atgacctgat
     5341 taacctcttg gggtagtgct tttatagaag aataaatatt ctactaattt aatacatcac
     5401 ctaaaatagt attgtttttt atggttggtt gaaggtcgtg gtgatgatgt tagtcctttt
     5461 gagaatattc cagtgccaga tcttacttat aataaagggg cttggcagtt tggataaact
     5521 attgttctta ttccactaat acaaactttt tgtaaagtat attaaatata cttcatttca
     5581 tactgaaatc cccggaagta ttcaatcaaa caattacacc ataaaaggaa gagggggccc
     5641 taattaagtt gtcaagcatg tttatgatac ctgcagggta taaattttct ggctgcaagc
     5701 agacacatat aaaacgtctt tattgatcct acccacataa tatggacaca gtcgtaagtg
     5761 aggctctggt tttcaaattg ttccggactg agaccaccac accaacggtg ccgtcgccag
     5821 cgattgtaat cgcactcgat ataactaaac actgttgccc gcactatttc ccgatggata
     5881 atagtgttct ccgtggagac attccatttt cagcgaatga attttcatct gttgctgtat
     5941 ccagtcatac agtgactgca tcagtggcgc ggctctggct tttcttgccg ccagacgctg
     6001 ttctgctgaa catccccgga cttctgcctc gatggcatac agttcaccga tacgctgcag
     6061 ggcttccgtg gtgatgtcgg ttggcactct tgcatgcaca tcgtggattt ttctccggac
     6121 atgagccata catgcggctt ccgttattct gccggattcg tacaacgccc ggtaaccacc
     6181 gtaagcatcg gcctgaagca ctccgctgta accggccaga tgattctgtg gatggatacc
     6241 tttccggtcc ggtgagtacg cgaaccagac cgccggaggc atttccgaac cggcgttacg
     6301 gtcatcacga acgtagaccc acagccgggc agtccttgtt ttaccgctgc ccggctcctg
     6361 gaccggaacg gg
//
LOCUS       DADLTY010000212         6357 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.214, whole genome shotgun sequence.
ACCESSION   DADLTY010000212 DADLTY010000000
VERSION     DADLTY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.214"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(76..972)
                     /gene="yihU"
                     /locus_tag="JN334_003758"
     CDS             complement(76..972)
                     /gene="yihU"
                     /locus_tag="JN334_003758"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HAY7439743.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            1140..2036
                     /gene="yihV"
                     /locus_tag="JN334_003759"
     CDS             1140..2036
                     /gene="yihV"
                     /locus_tag="JN334_003759"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HAY7439744.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            2070..2855
                     /locus_tag="JN334_003760"
     CDS             2070..2855
                     /locus_tag="JN334_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7439745.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            2954..3553
                     /gene="yihX"
                     /locus_tag="JN334_003761"
     CDS             2954..3553
                     /gene="yihX"
                     /locus_tag="JN334_003761"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HAY7439746.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            3547..4419
                     /locus_tag="JN334_003762"
     CDS             3547..4419
                     /locus_tag="JN334_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HAY7439747.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            4416..4853
                     /gene="dtd"
                     /locus_tag="JN334_003763"
     CDS             4416..4853
                     /gene="dtd"
                     /locus_tag="JN334_003763"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAY7439748.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            4898..5839
                     /gene="fabY"
                     /locus_tag="JN334_003764"
                     /gene_synonym="yiiD"
     CDS             4898..5839
                     /gene="fabY"
                     /locus_tag="JN334_003764"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HAY7439749.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         1505 a         1538 c         1722 g         1592 t
ORIGIN      
        1 tggcaaaacc gcccgatgcg cgcgtaatgt cgttgatggt gtacttattc attcatttat
       61 tcctttatcc agtcgttaca tttttacttt ggcagtcatc ccggcactga cacggacctg
      121 ttccagaatg gcggaccagt cctggcgacc gcgacccgct gcgcgcgcct ggctataaac
      181 ctcccgtgag gcggccccca gcggcattgg cacatgcagc tggttggcga catcaagggc
      241 gatgccaaga tccttatggg caagatcgat catgaaggcg ggagaaagat ctccgctgag
      301 gactttgttt ggccaggaag tggtgaagtg acctttaccg gcggcggtac cgctcatcac
      361 tttgacggca acatcgaagg gaagattcag ggcttcgcac aaaacggcgg cttctgccga
      421 aagcgcattg agcgcgatgc tcatgtagtt gttgatgagc ttaacgcgga tccccatgcc
      481 cggaccgcca gcgttgatca actcactgcc catcgccatc aggatcggtg tggcacgttc
      541 aacttgttca gcggtgccgc cagccagcag taacagagta ccggtaatgg catttgcaga
      601 agtacggcct accggaacat ccatcatgct gaagcctttg gcttgcatat cggcaatcaa
      661 tttatcggtt tgcagcggat ggatggtgga catatcaatg accagcgcat cggtagataa
      721 gccttcgcaa acgccgtttt caccgaacaa cacgttgcgc accagatcgc cattcggcaa
      781 catggtaatg ataaattcgg catctttagc ggcctgcgcc gggttggcgg cgggagtcgc
      841 acctttgtct accagatgcc gcacagcttc ggcgttcaca tcaaagacgc gaagttgatg
      901 cccttgctgc aataaattgc tcgccattgg cgaacccatt tgtcctaaac cgataaacgc
      961 gattgctgcc ataaccctct cctgaataca gtgatgtcac tttttgtcat ttatgacatg
     1021 ctttgcttgt ctgtttttga tcgaatttgt aatttatcgt caaaaaattg acagccgtca
     1081 ctttttaaac aattggtgaa attaaaatga acgcatccca aaatgtttaa ggaatgacca
     1141 tgattcgtgt tgcttgtgta ggtataaccg tgatggatcg catctattac gtggaagggt
     1201 taccgacgga gagcggtaaa tacgtggcga gaaattatac ggaagttggt ggcgggccag
     1261 cggcgacggc agcggttgcg gcggcaaggc tgggggcgca ggtcgatttt attggtcgcg
     1321 taggtgatga cgacgcgggc aacagcctgc tggcagagct ggaatcctgg ggcgttaaca
     1381 cccgttacac caaacggtat aaccaggcga aatcttcgca atccgccatc atggtggata
     1441 ccaaaggcga gcggataatc attaactacc ccagcccgga tctgctgcct gacgcagagt
     1501 ggttggagga aattgatttc tctcagtggg atgttgtgct ggcagatgta cgctggcacg
     1561 acggcgctaa aaaagccttc accctggccc gtcaggcggg tgtgatgacc gttctggacg
     1621 gggacattac gccgcaggat atcagtgagc tggtggcatt aagcgatcac gcggcctttt
     1681 cagaaccggg tctggcgcgc ttaacgggcg tgaaagagat ggccagtgcg ctaaaacagg
     1741 cacaaacgct cacaaatggt catgtctatg tgacccaggg tagcgcaggc tgcgactggc
     1801 tggaaaatgg tgggcgtcag catcaaccgg ccttcaaagt tgatgtggta gataccacag
     1861 gtgcgggaga tgtttttcac ggcgctttgg cggtggcgct ggcaacaagt ggggatttag
     1921 cggagtcagt ccgcttcgcc agcggtgtag cggcgttaaa atgcacacgt cccggtggac
     1981 gagccgggat ccctgactgt gatcaaaccc gatctttttt gtcacttttt gtataaaatg
     2041 ccagggtgat ggtttttcga ggaattttca tgagccttac cgaactaacc ggtaacccgc
     2101 ggcacgacca actcctcatg ctgatcgccg agcgtgggta tatgaatatt gatgagctgg
     2161 caaatctgct ggatgtctcc acgcagacgg tccgccggga tattcgtaaa ttaagcgagc
     2221 aaggcctgat tacgcgccat cacggtggcg cgggtcgggc ttccagcgtc gttaatacgg
     2281 cgttcgagca gcgtgaggtt tcgcaaaccg agaaaaaaaa agcgattgcc gaagcggtgg
     2341 cagactatat tcctgatggt tcaacaatat ttatcaccat tggtacgact gttgagcatg
     2401 ttgcccgggc gttacttaac cataatcatt tgcggataat caccaacagc ctgcgtgtgg
     2461 cgcatattct ttaccacaac ccgcgctttg aagtgatggt gcccggcggt acgttgcgct
     2521 ctcataatag cgggatcatt ggcccttcag cggcgtcctt tgtggctgat tttcgtgctg
     2581 attatctggt aacaagcgtt ggggcgattg agagcgatgg cgcgttgatg gagtttgatg
     2641 taaacgaagc taacgtggtg aaaacgatga tggcgcacgc gagaaatatt ctgctggtcg
     2701 ccgatcacac taagtatcat gcttcggcgg cggttgaaat tggtaacgtg gcacaggtca
     2761 ctgcgctctt taccgacgag ctgccgcccg ctgcgctaaa atcacgctta caagacagcc
     2821 aaattgaaat cattcttccc caggaagacg cgtagatttt tgtgaccgtt aacctggctt
     2881 cataccttgc cacatagcca aacccatcct ttcccgctac agttaatttc ttgtggcgcg
     2941 aaaggaggca aaaatgctct atatctttga tttaggtaat gtgattgtcg atatcgactt
     3001 taaccgtgtg ctgggagcct ggagcgattt aacgcgtatt ccgctggcat cgcttaagaa
     3061 gagttttcat atgggggagg cgtttcatca gcatgagcgt ggggaaatta gcgacgaagc
     3121 gttcgcagag gcgctgtgtc atgagatggc tctaccgcta agctacgagc agttctctca
     3181 cggctggcag gcggtgtttg ttgcgctgcg cccggaagtg atcgccatca tgcataaact
     3241 gcgtgagcag gggcatcgcg tggtggtgct ttccaatacc aaccgcctgc ataccacctt
     3301 ctggccggaa gaatacccgg aaattcgtga tgctgctgac catatctatc tgtcgcaaga
     3361 tctggggatg cgcaaacctg aagcacgaat ttaccagcat gttttgcagg cggaaggttt
     3421 ttcacccagc gatacggtct ttttcgacga taacgccgat aatatagaag gagctaatca
     3481 gctaggcatt accagtattc tggtgaaaga taaaaccacc atcccggact atttcgcgaa
     3541 ggtgttatgc taaaaaccat tcaggacaaa gccaggcacc gtacccgtcc actatgggcc
     3601 tggctaaaac tactctggca acgtattgat gaggacaaca tgacaaccct ggcaggtaac
     3661 cttgcctatg tgtcgttgct ctcattagtg ccgctggttg ccgttgtttt tgcgcttttc
     3721 gccgcttttc ccatgttttc cgacgtcagc attcagttac gtcactttat ttttgccaac
     3781 tttctgcctg ctactggcga tgttattcag cggtatatcg aacaatttgt tgctaattcc
     3841 aacaagatga ccgccgttgg ggcgtgcggg ctgatcgtca ccgcgttatt gttgatgtac
     3901 tccatcgata gcgcgttgaa taccatctgg cgcagtaaac gagcgcgacc aaaaatttac
     3961 tcgttcgccg tgtactggat gattttaacg ctggggccgc tgctggcagg ggccagtctg
     4021 gcgatcagtt cctatttgct ctccctgcgc tgggcgagcg atctcaatac tgtcatcgac
     4081 aacgtgctgc gtatttttcc gttgctgttg tcgtggatct ccttctggtt gctgtacagc
     4141 attgttccta ccatccgcgt acctaaccgc gacgcgattg tcggcgcgtt tgtcgccgca
     4201 ctcctgttcg aagcaggaaa gaaaggtttc gcgctttata tcaccatgtt cccgtcatat
     4261 cagctcattt acggtgtgct ggcggtgatc cccattctct ttgtttgggt ctactggaca
     4321 tggtgtatcg tcttgcttgg cgcggaaatt actgtcactc tcggggaata ccgcaaacta
     4381 aaacaagcag ctgaacaaga agaagacgac gaaccatgat tgcattaatt caacgcgtaa
     4441 cccgtgccag cgtcaccgtg gagggagaag tgacgggcga aattggcgcg ggacttttgg
     4501 tgttattggg tgtcgaaaag gatgacgacg aacagaaagc aaaccgtctg tgcgagcgtg
     4561 tgctcggcta ccgcatcttt agcgatgccg aaggcaagat gaatctcaac gtgcaacagg
     4621 cgggcggcag tgtactggtg gtttcccagt ttaccctcgc cgcagatacc gaacggggga
     4681 tgcgcccaag tttctccaaa ggcgcatcac cggatcgcgc agaggcgtta tatgactatt
     4741 tcgtcgaacg ctgccgccag caagagatga acacgcaaac aggacgcttc gctgccgata
     4801 tgcaggtatc gctggtcaat gatggccccg tgacattctg gttgcaggta tgagccagct
     4861 tccagggttg tcacgggaaa caagagagag tatcgctatg tatcaccttc aggttccaca
     4921 aacagaagaa gagttagagc gttactatca ctttcgctgg gaaatgttgc gtaagcccct
     4981 gcatcaacca aaaggttcgg aacgcgacgc gtgggatgcg atggcgcatc accagatggt
     5041 cgtcgacgag cagggtaatc tggtggcggt aggccgattg tatattaatg ccgacaatga
     5101 agcgtccatt cgctttatgg tcgttcaccc ggacgtgcag gacaaagggt taggaacgct
     5161 gatggcgatg accctggagt cggtggcgcg tcaggaaggc gttaagcgcg tgacctgtag
     5221 cgcccgtgaa gacgcggtgg agtttttcgc caagctgggg tttgttaatc agggagaaat
     5281 caccacgcca accaccacgc cgattcgcca ttttttgatg attaagcccg tcgccactct
     5341 ggatgatatt ttgcatcgcg gcgactggtg cgcgcagctg caacaggcgt ggtacgaaca
     5401 tatcccgctt agtgaaaaaa tgggcgtgcg tattcaacaa tataccgggc aaaaatttat
     5461 caccaccatg ccggaaaccg gcaatcagaa tccgcaccat acgctgtttg cggggagttt
     5521 attctcgctg gcgacgctca ccggttgggg gcttatctgg ctgatgctgc gtgaacgcca
     5581 cctcggcgga acgattattc tggcggatgc gcatatccgc tacagcaaac cgattagcgg
     5641 taaaccccat gcggtagccg accttggtgc cttaagcggc gatctcgacc gtctggcgcg
     5701 cggacgaaaa gcacgggtgc agatgcaggt cgaaatcttt ggcgacgaga cgccgggtgc
     5761 agtgtttgaa ggcacgtata tcgttctgcc cgcgaagcca tttggcccgt atgaagaggg
     5821 cgggaacgaa gaagagtagc ttatggagag gggcggtgct gcctctctca ttcattccgg
     5881 tcatttacat cagtccctga ctattccggt tatattgaat aagaacttta taaattttta
     5941 ttcttcgttg gcaccatacg tgtcttgcta catcactgtt caggagcatt taaatattat
     6001 ttgtagagct gagtggcaat tattgcagat aatgcttaac tcatcatatt agttcaggtt
     6061 tgagcgattt ttgatagtca aaaccccctc tggtgccata cttttttcat aaatattttt
     6121 aaaatctata gctcacaaat attatgcaaa gataatgggc tggcatacat tgtcagtatc
     6181 attcatttac tgctctgtat gttatgaatc attatgttgt atgtttgctc ttaaaccgtg
     6241 ggaaggaggc tataatggtt gccaatatta atctcattaa acaagaaagt tattctgttg
     6301 ttaatttaga aaaacaacta atgagtaata aatcaccgaa gcaaacgcta ttaaatc
//
LOCUS       DADLTY010000213         6319 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.215, whole genome shotgun sequence.
ACCESSION   DADLTY010000213 DADLTY010000000
VERSION     DADLTY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.215"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(274..1305)
                     /gene="rsmC"
                     /locus_tag="JN334_003765"
     CDS             complement(274..1305)
                     /gene="rsmC"
                     /locus_tag="JN334_003765"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HAY7439750.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1408..1821
                     /gene="holD"
                     /locus_tag="JN334_003766"
     CDS             1408..1821
                     /gene="holD"
                     /locus_tag="JN334_003766"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HAY7439751.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1790..2236
                     /gene="rimI"
                     /locus_tag="JN334_003767"
     CDS             1790..2236
                     /gene="rimI"
                     /locus_tag="JN334_003767"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAY7439752.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2251..2928
                     /gene="yjjG"
                     /locus_tag="JN334_003768"
     CDS             2251..2928
                     /gene="yjjG"
                     /locus_tag="JN334_003768"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HAY7439753.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3019..4608
                     /gene="prfC"
                     /locus_tag="JN334_003769"
     CDS             3019..4608
                     /gene="prfC"
                     /locus_tag="JN334_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAY7439754.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            5001..5606
                     /gene="osmY"
                     /locus_tag="JN334_003770"
     CDS             5001..5606
                     /gene="osmY"
                     /locus_tag="JN334_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HAY7439755.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5723..5884
                     /locus_tag="JN334_003771"
     CDS             5723..5884
                     /locus_tag="JN334_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAY7439756.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6006..>6319
                     /locus_tag="JN334_003772"
     CDS             6006..>6319
                     /locus_tag="JN334_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAY7439757.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LI"
BASE COUNT         1547 a         1617 c         1680 g         1475 t
ORIGIN      
        1 ccatcaattc ttaaaaagaa ttgctaccac ggaggcgcat tctagtggtt ttcagctttt
       61 cgtcaatagt taattatcgc ctgatgtgta attgctggaa aaatgtccat caggaaacta
      121 gcgtgcaggt ttggtaggca tgcgggggca gatgccagat gcgacgctgg cgcgtcttat
      181 ctggcctacg aagggctaac gtgcaggttt tgtaggtcgg ataaggcgtt cacgccgcat
      241 ccgacacggt atgcggcgac agatatcaga tactcaacct ttcttcgcct ggcgggtcat
      301 aatggcgcga tataccttga agcgcccggt ttgcgcgata acttcatgga agccaaatgt
      361 ctcatccagc acgtccgggt aaggcaggaa ggcgttcgct acaattcgca gctcgccgcc
      421 gctattaaga tgacgcaccg cgccacgaat cagcgtttgc gccgcatcca ggctggtttg
      481 catcccatcg tggaacggcg ggttggagat gatcatatca aaacaacctt tcacctcgga
      541 aaagacgttg ctggcaaaga cttcaccttc aacaccgttg gctgcaagtg ttgcgcggct
      601 ggcttctacc gccggggcag agacatcgca caaggtgaga cgaattttcg gcgaatggcg
      661 cgcaaaggca actgaaagta cacccgcgcc acagccgaca tccagcactt tacctttcgt
      721 gtgcggagtt aacgtcgaga gcagcaactg gctaccgaca tccagaccgt cgcggctaaa
      781 cacgccaggc agcgttttga ccgtcaggcc atcgacgctg tattcgcccc agaatttatc
      841 cgcatcaaat actggctgtt tttccagacg accaaaatag aggccacagc gacgagcgct
      901 gtcgacttta ttcaacggcg cataatctgc cagcatctgc tcagcgctgc gcacgccgct
      961 gcggttctcg ccaacgacaa aaatatctgt ccccactggc agcagagaaa gtaaattcat
     1021 caactggaac tgggcttccg gtttgttctt cggccagtag taaatcagcg tatcgcaatc
     1081 tgcgacatca ttcgccgtgg cgaccagact aaaacgggcg ttatccccca tctggcagct
     1141 taacacctgc caatggtgga attgctgggt atgagcacgg ctggccgcgg tatctaaacg
     1201 cgcgggcagg tcatcctgta agtctccggc aaacagaata cggctttgtt cgaaatcatc
     1261 actgtgacgc agcaagactt cacttgccgg ggtaaatgca gacatggaat gctcctcaat
     1321 tgatactggc ggcgattata gccatatgtt ggcgcggtat cgacgaattt gctatatttg
     1381 cgcccctgac aacaggagcg attcgctatg acatcccgac gagactggca gttacagcaa
     1441 ctgggcatta cccagtggtc gctgcgtcgc cctggcgcgt tgcaggggga gattgccatt
     1501 gcgagcccgg cacacgtccg tctggtgatg gtggcaaacg atcttcccgc cctgactgat
     1561 cctttagtga gcgatgttct gcgcgcatta accgtcagcc ccgatcaagt gctgcaactg
     1621 acgccagaaa aaatcgcgat gctgccgcaa ggcagtcgct gcaacagctg gcggttgggt
     1681 actgacgaac cgctatcatt ggaaggcgct caggtggcat caccggcgct caccgaatta
     1741 cgggcaaacc caacggcacg cgccgcgtta tggcaacaaa tttgcacata tgaacacgat
     1801 ttcttccctc gaaacgactg atttaccagc ggcttaccac attgaacaac gcgcccacgc
     1861 ctttccgtgg agtgaaaaaa cctttgccag caaccagggc gagcgttatc tcaactttca
     1921 gttaacgcaa aacggcaaaa tggcggcgtt tgcgattacg caagtggtgc tggatgaagc
     1981 tacattgttc aatattgcgg tcgatcccga ctatcagcgt caggggctgg gtagggcgct
     2041 gctggaacat ctgatcgacg aactggaaaa acgcggcgtg gcgacactat ggctggaagt
     2101 ccgtgcctca aatgctgccg ccattgccct gtacgaaagt ttaggcttta acgaggcgac
     2161 gattcgccgc aattactacc ccaccacgga cggtcgcgaa gacgccatca tcatggcgtt
     2221 gccaatcagt atgtaagaca aggtggaata atgaagtggg actggatttt ctttgatgcc
     2281 gatgaaacgc tgtttacctt tgactcattc accggcctgc agcggatgtt tcttgattac
     2341 agcgtcacct ttaccgctga agattttcag gactatcagg ccgttaacaa gccactgtgg
     2401 gtggattatc aaaacggcgc gatcacttca ttacagcttc agcacgggcg tttcgagagc
     2461 tgggccgaac ggctgaaagt tgaagcaggc ttgcttaacg atgcctttat taatgcgatg
     2521 gcggaaatct gtacgccgtt gccgggcgcg gtttctctgc ttaacgccat tcgtagcaac
     2581 gctaaaatcg gcatcatcac caacggtttt agcgccttgc agcaagtgcg tctggaacgg
     2641 acgggcctgc gtgattactt cgatttgctg gtgatttccg aagaagttgg cgttgccaaa
     2701 ccgaataaga aaattttcga ttatgcgctg gaacaggcgg gcaatcctga ccgttcacgc
     2761 gtgctgatgg ttggcgacac tgccgagtcc gatattctcg gtggcatcaa cgccgggctt
     2821 gcgacctgct ggctgaatgc gcacaatcgc gagcaaccag aaggcatcgc gcccacctgg
     2881 accgtttctt cgttgcacga actggagcag ctcctgtgta aacactgatt gcctcccccc
     2941 cgttgatggg taaaatagcc gcaatttttc gttttcaaca agcgcggcgc gatgccgctt
     3001 actcaagaag aaagaattat gacgttgtct ccttatttga aagaggtggc gaagcgccgc
     3061 acttttgcca ttatttctca cccggacgcc ggtaaaacta ccatcaccga gaaggtgctg
     3121 ctgttcggac aggccattca gaccgccggt acagtaaaag gccgtggttc caaccagcac
     3181 gctaagtcgg actggatgga gatggaaaag cagcgtggga tctccattac tacgtctgtg
     3241 atgcagtttc cgtatcacga ttgcctggtt aacctgctcg acaccccggg gcacgaagac
     3301 ttctcggaag atacctatcg taccctgacg gcggtggact gctgcctgat ggttatcgac
     3361 gccgcaaaag gtgttgaaga tcgtacccgt aagctgatgg aagttacccg tctgcgcgac
     3421 acgccgatcc tcacctttat gaacaaactt gaccgtgata tccgcgaccc gatggaactg
     3481 ctcgatgaag ttgagaacga gctgaaaatt ggctgcgcac cgatcacttg gccaattggc
     3541 tgcggcaagc tgtttaaagg cgtttaccac ctttataaag atgaaaccta tctctatcag
     3601 agcggtaaag ggcacaccat tcaggaagtc cgcattgtta aagggctgaa taacccggat
     3661 ctcgatgctg cggttggtga agatctggca cagcagctgc gtgacgaact ggaactggtt
     3721 aaaggcgcgt ctaacgagtt cgacaaagag ctgttccttg cgggcgaaat cactccggta
     3781 ttcttcggta ctgcgctggg taacttcggc gttgatcata tgctgaatgg cctggtggag
     3841 tgggcgcctg cgccgatgcc gcgtcagacc gatacccgta ccgtagaagc gagcgaagat
     3901 aaatttaccg gcttcgtatt taaaattcag gccaacatgg atccgaaaca ccgcgaccgc
     3961 gtggcgttta tgcgtgtggt gtccggtaaa tatgaaaaag gcatgaaact gcgccaggtg
     4021 cgcactgcga aagatgtggt gatctccgac gcgctgacct ttatggcggg tgaccgttcg
     4081 cacgttgaag aagcgtatcc gggcgatatc ctcggcctgc acaaccacgg caccattcag
     4141 atcggcgaca cctttaccca gggtgagatg atgaagttca ccggtattcc gaacttcgca
     4201 ccagaactgt tccgtcgtat ccgcctgaaa gatccgctga agcaaaaaca gctgctcaaa
     4261 gggctggtac agctttccga agagggcgcg gtgcaggtgt tccgtccaat ctccaacaac
     4321 gatctgatcg ttggtgcggt tggtgtgctg cagtttgatg tggtggtagc gcgcctgaag
     4381 agcgaataca acgttgaagc agtgtatgag tcagtcaacg ttgccactgc ccgctgggta
     4441 gaatgtgcag acgcgaagaa attcgaagag ttcaagcgta agaacgaaag ccaactggcg
     4501 cttgatggcg gcgataacct cgcttacatc gctaccagca tggtcaacct gcgcctggca
     4561 caggaacgtt atccggacgt tcagttccac cagacccgcg agcattaatt cttgcctcca
     4621 gggcgcggta gccgctgcgc cctgtcaatt tcccttcctt attagccgct tacggaatgt
     4681 tcttaaaaca ttcacttttg cttatgtttt cgctgatatc ccgagcggtt tcaaaattgt
     4741 gatctatatt taacaaagtg atgacatttc tgatggcgtt aaataccgtt caatgcgtag
     4801 atattagtat ctaaagccgt cgattgtcat tctaccgata ttaataactg attcagaggc
     4861 tgtaatggtc gttattcatc acccatcgct tttgtgatgg cgaccattga cttctgtaga
     4921 gggtgaagtc tctccctatt cagcaatgca acctcgtgtt gctaggctca aattacgagc
     4981 aaacatacag gaataaatcg atgactatga caagactgaa gatttcgaaa actctgctgg
     5041 ctgtaatgtt gacctctgcc gtcgcgaccg gctctgccta cgcggaaaac aacgcgcaga
     5101 ctaccaatga aagcgcaggg caaaaagtcg atagctctat gaataaagtc ggtaatttca
     5161 tggatgacag cgccatcacc gcgaaagtga aggcggcgct ggtggatcat gacaacatca
     5221 agagcaccga tatctctgtc aaaaccgatc aaaaagtcgt gaccctgagc ggtttcgttg
     5281 aaagtcaggc ccaggccgaa gaggcagtga aagtggcgaa aggcgttgaa ggcgtgacct
     5341 ctgtcagcga caaactgcac gttcgcgacg ctaaagaagg ctcggtgaag ggctacgcgg
     5401 gtgacaccgc caccaccagt gaaatcaaag ccaaactgct ggcggacgat atcgtccctt
     5461 cacgtaaagt gaaagttgaa accaccgatg gcgtggttca gctctccggt accgtagatt
     5521 ctcaggcaca aagtgaccgt gctgaaagta tcgccaaagc ggtagatggt gtgaaaagcg
     5581 ttaaaaatga tctgaaaact aagtaattcg tcctcccgaa ctttgtcggg aggcgcgatg
     5641 tgcaccacac taaaaatatc gcattgagtg attttcacgc tcatatcaag cggtgacatt
     5701 aactatggta aaggagacgc ttatgtttcg ttggggcatc atatttctgg ttatcgcgtt
     5761 aatcgccgcc gcacttgggt ttggtggtct ggccggtacc gctgcaggcg cagctaaaat
     5821 tgtctttgtc gtcgggatta ttctgttcct agtgagtttg ttcatgggcc gaaaacgacc
     5881 ctagatttca gaccattaag aaaattttat ccaaagccag tccagcggac tggcttttgc
     5941 ggttttagcg aataataaat tgcgttactt tgtctttctt tcacaacaaa aacaggaagg
     6001 cagaagtggg gcagcgaata cctgtaacgc ttggtaatat tgcgccgttg tcgctaaggc
     6061 cgttccagcc tggacgaata gctctggtgt gcgaaggcgg cggacagcgt ggaattttca
     6121 cggctggcgt gctggatgag tttatgcgcg cgcagtttaa tcctttcgat ctttatctcg
     6181 gcacatctgc cggggcgcag aacctctcgg cgtttatctg caatcagccc ggttacgcgc
     6241 gcaaagtcat catgcgctat accacaaaac gcgaattttt cgatccattg cgctttgtcc
     6301 gtggaggaaa tcttatcga
//
LOCUS       DADLTY010000214         6254 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.216, whole genome shotgun sequence.
ACCESSION   DADLTY010000214 DADLTY010000000
VERSION     DADLTY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.216"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            6..311
                     /locus_tag="JN334_003773"
     CDS             6..311
                     /locus_tag="JN334_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7439758.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(387..851)
                     /locus_tag="JN334_003774"
     CDS             complement(387..851)
                     /locus_tag="JN334_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY7439759.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(848..1723)
                     /locus_tag="JN334_003775"
     CDS             complement(848..1723)
                     /locus_tag="JN334_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HAY7439760.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1720..2409)
                     /locus_tag="JN334_003776"
     CDS             complement(1720..2409)
                     /locus_tag="JN334_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAY7439761.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2457..3947)
                     /gene="nanT"
                     /locus_tag="JN334_003777"
     CDS             complement(2457..3947)
                     /gene="nanT"
                     /locus_tag="JN334_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HAY7439762.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4057..4950)
                     /gene="nanA"
                     /locus_tag="JN334_003778"
     CDS             complement(4057..4950)
                     /gene="nanA"
                     /locus_tag="JN334_003778"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAY7439763.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5072..5863)
                     /gene="nanR"
                     /locus_tag="JN334_003779"
     CDS             complement(5072..5863)
                     /gene="nanR"
                     /locus_tag="JN334_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HAY7439764.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1584 a         1695 c         1552 g         1423 t
ORIGIN      
        1 ttgccatgat ttacattgaa cagcttgaat taatccataa aagtggtgat gtgttgtatc
       61 cagtaaaaat aaccagaaaa tcatcaggaa aaactgcctt ccaccttgtt ccattcggct
      121 taaataagac tcacgacctt ctagaggtgg aagatgcttc agaagcaatt cggctggtga
      181 ttgatgaacg tcattccatt cgctgcagta cacttacagc aactattacg aataaaaagg
      241 gcaagcgaat taagcggaca ggtatataca gtatcaaggg tgttaacata aaagaataca
      301 acgtcaggta atggcacaga gcggtccgtc agttcaggtt tggctctgtg ccaaagatgg
      361 gtcaggtcac acctgatcta ataaatttaa gccattaaat cagccttaac cttcaccacc
      421 acctttttaa tctcacccgg ctcgccaaca acgcatcccg gcttatgcgg ctctcctggc
      481 ataaacacgg cgaacattcc cggtttcagg atgatggttt gctcgttttc aatggcgcta
      541 caaagctggt aatcatcttc atggtggaac tcttcacatt ggcgcgcagt gcctgccatg
      601 ccaaacagaa tccgttcctc accgtttaat aacagctgga tatcaatgta ttgctcgtgc
      661 aattccgctt ttttctcgac gggcgattga gtgttaaacg tcatgacatt cataaaaata
      721 ttgtcgccct gtaattcgta acgacccggc gctttttctt gcggtctggc agctaaggcc
      781 agcgttaacg cgtcctgtaa cgcaggatgt aacccagcag acggtaatga ctgtacttca
      841 cccatcatca taatttttct ccctgggcca acagcgcagc cccaagtaaa cctgcatcat
      901 ggcggtaatg cgccgccagt aaatcaacat gaaatgccgc tggctcctgc gccagataca
      961 tttccaccaa cgccagatac ccttctgcca gaccaacgct gccaccaacc accacgcact
     1021 ggcaatcagt tgtggcttta atatcagcga tcagccttgc aagcgtacgt gcggagcgat
     1081 gaatcagttg ctgcgcctgc tcatcgccct gcccggcgcg tgtgaaaata gttctcgcat
     1141 ccgcgccagc cagctcgccc tgcgctgccg ccgcaatgcc gcgaccagaa gcaattgctt
     1201 ccacgcaacc tgtgcgtcca cagccgcaga ctgggccgtg tggatcggca agcgtatgcc
     1261 cgatgtgccc cgccagaccg ccagggccgg taagcagttt gccgccgctc actacaccgc
     1321 cgccgacgcc agtggaaacg gtgataaaga ccatatcggt tatatcgcct tccagcgcct
     1381 gatactctgc ccatgctgcg gcctgcgcgt cgttaatggc aatggtcggc aaattggtaa
     1441 gttgttccag cgttttgact aacggaaagt gtagcaatcc accgagatta tgcggattca
     1501 gcgccagcaa actgccgtcg cgaataattc cggttgaagc gatggcaacc cgctgcgcat
     1561 gagcttgcaa cggagagact aatgcggata aggcatcacg caaggcttct ggtgtctggc
     1621 tggctggcgt aggaagttca cgacgatcgc ggatctgccc gtcagcgcca atcagcgcgg
     1681 cggcaagttt agtaccgccg atatcaatcg ccagtatggt catagcaccg cctttttcat
     1741 cgctgtgttg taccactgac aaatgtgttc aagacgcgta atggcagaac cgaccgtcac
     1801 cgcccacgcg ccgtggcgca tcgcatctgc cgcctgagca ggcgtgttgt aacgcccttc
     1861 ggcaatcacc cgacatccgg cttcgctcaa cgttttcacc agcgccagat ccggctcttc
     1921 tggcgtttca ggcgtggtat agccagaaag cgtagtgcca ataatttcgg ctcccagctt
     1981 ttggcatgcc aggccgtctt ccggcgttga gcagtcggtc atcgccagta aaccgtgatg
     2041 atggatacgt gccagcagcg tttcaacagg caccggacgc gggcggtcgg tgccgtcaat
     2101 ggcgataatg tccgcgcccg cctgcgccag cgcatcgaca tcttcaatat aggccgtgat
     2161 gcgtaccgga gaatcctcca gatcgcgttt cacaattcca ataatcggca cgctcaccac
     2221 cgcacgcgtg gcttgcagat ttgccacacc ttcgatgcga atggcaaccg cgcccgcctg
     2281 ctctgccgct aatgccatgg ctgcgacgat ttcgggttta tcgagcgggc tgtcaggaac
     2341 cggctggcag gagacaatca ggccaccgtt agcagcgatt ttttgatcca gttgtgcaag
     2401 taacgacata catcttccct tagcgaaagg cccggtacat agaccgggca acaggattaa
     2461 cttttggttt tgactaaatc gtttttggcg ctgccaaatg gcacggcacc gctaaatggt
     2521 ttgccgtcga tagcgtcatg agtacgcaat gcttccgggc gcagccaacg ctgaacgcgg
     2581 gaaggcatat ccagaccaat cagcaggatc accacaaatg tcaggctgaa cgagagcgat
     2641 gccagcgcag tacccagatc cagacgttga gcgatcaacg cgccgatgat tggggccagt
     2701 gcgccaccca atgcgccaac gttgtaggta aagcccaggc ccgctgcacg ctggtcggta
     2761 tcgaaataac cgccaatcag tttcggtaag atcccggcga tcccttgtcc aagcatttgc
     2821 tggaagaaca gtaacagacc gagcacccag acgtttgcgc cgccaatcgc aaataccgga
     2881 ataatcagca gctgcgaggc cagcaggcta caaacgtacg ctttgcgggt tcccagccag
     2941 tcaccgagga agccacctac gcagcatccc acggccgcgc caaagccact aaagaacagc
     3001 acattggcga cagtatgcgg gttataagcc agatcggttt tcagatacgt tggcagcagc
     3061 gcctgaatcg gccatgagta gaggaaagca aacaacacga ccaccatcag cattacgccc
     3121 gttggccagc gtttacccgt actttgcacc ataaagctga taaagattgc ggcgcataac
     3181 agcccgagaa cagcgacgat tgcagcattt tgcaggttac cggcgaagca gaaccacagc
     3241 gcagtagccg ccgccagtgt cattacgata ttggcaatgc gatgttcacc acggtagaga
     3301 atatccacca ttgtgcgtac tggtgcttta cctgcgtgtt tctctttcca gtcttccgct
     3361 tccgggatgt ttttacgcag ccagagagca aagatgattg gcaaaatgcc gataaagaac
     3421 agcgcacgcc aaccccagac cggaaccacc aggctataga cctgagcggc aacgacggcc
     3481 cccacagaga agcctgaaat caaaaaacca ctggctttgt tacgcagatg ttttggccag
     3541 ctttcaatga cataggtggc gctggaaccg tattcacccg ccatccccat gccgatgacc
     3601 agacgagcga taaacatggt gatgtagcct ggcgcaaagc cgcaggccag cgtcccggcc
     3661 gagaagagaa cgatgctggt gaccattgcc agacgacgcc cgtagcggtc acccatagcg
     3721 ccgagcatca ggccgccgaa ccagcgagag ataaaggctg cggagatcag acttgccgcc
     3781 tgcaccgtcg tcagcccgaa ttcaccctgt acttcggtga gtaccagggc gattaaaacg
     3841 aaatcaaaac cgtcaagcag atatcccaac caggcagcgg aaaatgcgcg ccattgtgca
     3901 cggttgagat ggcgatacca cgggatattc tgggttgtag tactcattgt gagtctcccg
     3961 cggtgggcga tgcccacacg ctttggtatg aaaattgtag ggtacagatg cgtttatttc
     4021 ccctcacccg gtaggggcga gcgaggggaa aacaactcac ccgcgctctt gcatcaactg
     4081 ctgggccagc gccttcagtt ctggcagata tttttcatct accggtccaa acggtttgcg
     4141 gcacagcggc acagaaacga catccatata atgcaggaca gttttcaggc cgcggaatac
     4201 gcccgttttg atcagtaaat caatgacttt gttgcactca gtttgcagtt tctgcgcggt
     4261 ctggatatcg ccttctttca gcgccttcac gatcccctga tatcgccagc ccatgatgtt
     4321 gtaagtactg ccgataccac catcagcgcc tgccagcaag ccggaagcga agatttcgtc
     4381 gtaaccgtta tagagcacca gatcaggatg ttcacgacgg atctgttcca tctgatagag
     4441 atcgccagaa gtctgtttca gcgcaccgac gccaggcaac gtaaccaggg tgttgatctg
     4501 atccagggtc agttttaccc cactcagggc tggaatgttg tacaccacca tcggcaaacc
     4561 atccgctgaa tcaataattg cccgatagtg atcgcagtgt tcttcaaagc tgaaaggata
     4621 gtagaacggc gtgacggcgg agacggcatc gaagccataa cgtttagccg atgccgcaag
     4681 ttgttggctt tcggcggtgc tgacgcaacc gacgtgggcg atgagtttaa tcttaccttt
     4741 cgcctcttcg gcgacgattt ccagtacctg ttcacgctcg gaaaggcttt gtacaaaggc
     4801 ttcgccggtc gaaccaccca cgtataaacc gtcgatgccc tgctgaatat tgaactgaac
     4861 caggcggcgc agactcgctt tatccagtgc ttgttgttga tcaaaaggag tcaggagtgc
     4921 agccattacg ccacgtaaat tcgttgccat aaatacctct gaagtgatgc ttgtctgata
     4981 aacgatatac ctttatacct gttataccag atcaattaag caacacccca tacagaaagc
     5041 ttataatgcg atctgcttca ctaaagtggc attatttctt tttgttggtg gtctgaccga
     5101 aagcgtgcca ggtagcagag acgctgttga gatgcgattg caacgcacga tcggcttcgt
     5161 caggatcatg acggcggatc gcatcaacga tcgcaatatg ctgttgataa ctaacgttgt
     5221 tatgttcgtg cagtgcctga tcggtaaccg ttgggcgtgc ggcaataagc cagtcgagca
     5281 gggcaacgtg gatcgccatg aagattgggt taccggggat ctccgccagc acgcggtgga
     5341 aatcaacgtc tgaacgaatg aatgccgcgt tgttatccag cgactgactg ttgatttcca
     5401 gtgcttttgc cagcaaatcg atttgctcat cggtggcgtg ttcagccgca tagcgcacca
     5461 ggctggattc aaagaacaga cgtaattgtt cgaaatgggc aatcccacca ggatgagaaa
     5521 ggaaatcttt cgccatgccg gaaagctcgc cgatgatagt gtccgcagaa ggtcgagaga
     5581 cacgagcgcg ttcgccgttg tttatttgca ccagaccttt gcgttttaac gctgccagcg
     5641 cttcacgcac agaaggacgc ccgacgttaa agaacgccat cagttcgcgt tcagacggta
     5701 attgttcacc ttcgccaaat tcacgacggc ggatcatctg ttccagctct tcttccacca
     5761 tttcggagag ttttttacgc gccagcgggc ggctacgcaa gttgcgacca attgcaggtg
     5821 aagaatcttc ggtttgcgaa tcaaatgcgt tcataaggcc cattctgtaa ggtcagtgtg
     5881 attaacatca tcagtgacat cctatcacag gattgaaagt aggggaaaat ggcaggtttt
     5941 tctctttgtg cctcatcatt accataatta acggaataat taactattgc gaaaaattaa
     6001 tgtaacgcag ataaacacat cccgattgaa ttattcataa gactattcac aagcattatg
     6061 aatattatga atgtgttctt acaaaataat cataatcgca tattttttaa tgaaaaatca
     6121 cctcacctac aattaaaaac acgacatccg caccataaat agacttgcaa aaaatataac
     6181 atcattgttt tcaatctgcc atttatggga ttgaccgttt tcttttgaca cggagttcaa
     6241 caatgggtaa tgct
//
LOCUS       DADLTY010000215         6253 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.217, whole genome shotgun sequence.
ACCESSION   DADLTY010000215 DADLTY010000000
VERSION     DADLTY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.217"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="JN334_003780"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="JN334_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HAY7439765.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="JN334_003781"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="JN334_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HAY7439766.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /locus_tag="JN334_003782"
                     /pseudo
     CDS             complement(2659..5772)
                     /locus_tag="JN334_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(5797..6054)
                     /locus_tag="JN334_003783"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="JN334_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
BASE COUNT         1658 a         1458 c         1476 g         1661 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag atg
//
LOCUS       DADLTY010000216         6160 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.218, whole genome shotgun sequence.
ACCESSION   DADLTY010000216 DADLTY010000000
VERSION     DADLTY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.218"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..168
                     /locus_tag="JN334_003784"
     CDS             <1..168
                     /locus_tag="JN334_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7439767.1"
                     /translation="RINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPK
                     RYRKKLTEFNQ"
     gene            274..1626
                     /locus_tag="JN334_003785"
     CDS             274..1626
                     /locus_tag="JN334_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY7439768.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1639..2397
                     /gene="chbG"
                     /locus_tag="JN334_003786"
     CDS             1639..2397
                     /gene="chbG"
                     /locus_tag="JN334_003786"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAY7439769.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2444..4705)
                     /gene="katE"
                     /locus_tag="JN334_003787"
     CDS             complement(2444..4705)
                     /gene="katE"
                     /locus_tag="JN334_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HAY7439770.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4888..5151
                     /gene="cedA"
                     /locus_tag="JN334_003788"
     CDS             4888..5151
                     /gene="cedA"
                     /locus_tag="JN334_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HAY7439771.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5440..5926
                     /locus_tag="JN334_003789"
                     /pseudo
     CDS             5440..5926
                     /locus_tag="JN334_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394351.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1579 a         1310 c         1559 g         1712 t
ORIGIN      
        1 cgtattaatt ttgcaaaaaa acaactggaa atgaccaact attccgtgac ggatattgcg
       61 tttgaggccg gttatagtag cccgagtttg tttattaaaa cgtttaagaa attaacgtcc
      121 tttacgccta agagatatcg taagaaattg actgaattta atcagtaagt cgttatacct
      181 gacaattcac atatctgtcc tgttgctgga ttatttatgt ccgggggggg cagatatgcc
      241 agatatcagt attctgtact gaagggagaa attatgagcc agaaattaaa agtcgtcact
      301 attggtggcg ggagcagcta taccccggag ttactggaag gatttattaa gcgttatcac
      361 gaattgccgg tcagcgaatt atggctggtg gatgtcgagg gtggcaaagc taaactggat
      421 attattttcg atctctgcca acggatgatt gataacgctg gcgtcccgat gaagctttat
      481 aaaacgctgg attgccgcga agcattgaaa gatgctgatt tcgttactac ccaactgcgc
      541 gttggccaat taccggcgcg tgaactggat gaacgtattc cattaagtca tggttatctt
      601 ggtcaggaaa ccaacggcgc gggcggtttg tttaaaggtc tgcgtaccat tccggtgatt
      661 tttgacatcg taaaagatgt cgaagaactt tgtccgaatg catgggtgat taacttcact
      721 aacccggcgg gaatggttac tgaagccgtt tatcgtcata ccggatttaa acgctttatc
      781 ggcgtgtgta atattccgat cggcatgaag atgtttattc acgatgttct gatgctgaaa
      841 gacagcgatg atttatctat cgatttgttt ggcctcaacc atatggtgtt cattaaggat
      901 gtgctggtaa atggcaagtc gcgctttgcc gaattgcttg atggtgtggc atccgggcag
      961 ttaaaagtat ctggcgttaa aaatattttc gatctgccat ttagcgaagg cttaattcgt
     1021 tctctgaatc tgttgccgtg ttcttatttg ctttattact tcaagcaaaa agagatgctg
     1081 gctattgaaa tgggcgaata ctacaaaggc ggcgcacgag cgcaggtagt acagaaagtc
     1141 gagaaacaac tttttgagct gtataaaaac ccggagttga aagttaagcc gaaagaactg
     1201 gaacagcgcg gtggggctta ttactctgat gcagcgtgcg aagtgatcaa cgctatctac
     1261 aacgacaagc aagctgaaca ttacgttaat atcccgcatc atgggcatat tgataatatt
     1321 ccggcagact gggcggtaga aatgacctgt aagctggggc gcgatggcgc gacgccacat
     1381 ccgcgcatta cgcatttcga tgataaagtg atggggctga ttcacaccat taaaggcttc
     1441 gagattgctg ccagcaacgc cgcacttagc ggagaattta acgatgtgtt actggcgcta
     1501 aaccttagtc cgttggtgca ttccgatcgc gatgctgagc tgctggcacg cgagatgatt
     1561 ctggcgcacg agaaatggct gccaaatttt gccgactgca tcgcagagct taaaaaagca
     1621 cattaaccga ggctgattat ggaacgctta ctgattgtta atgccgatga ttttggctta
     1681 agcaaaggtc agaactacgg cattatcgag gcctgtcgca atgggattgt cacatcgacg
     1741 acggcgctgg tgaatgggca ggctattgac catgcggtgc agttgagtcg tgatgaaccg
     1801 agtctggcca tagggatgca ctttgtcctt actatgggca agccactgac agctatgccg
     1861 gggttaaccc gcgatggtgt gctgggaaaa tggatctggc agttggcaga agaagatgct
     1921 ttaccgctgg aagaaattac tcaggagctt gccagtcagt atttgcgttt cattgagcta
     1981 tttggacgca aacctacgca tcttgatagc catcatcatg tgcatatgtt cccgcagatt
     2041 ttcccgattg ttgccaggtt tgcggctgaa gaggggattg cgttgcgcat cgaccgtcag
     2101 ccgctatcta acgctggtga tttaccggct aatctgcgca gttcgcaggg attcagtagc
     2161 gcgttttatg gtgaagagat tagcgaggca ctgttcctgc aagtgctgga tgacgcaagc
     2221 catcggggtg atccttcgct ggaggtgatg tgtcatccgg cgtttatcga taatactatc
     2281 cgtcagagtg cttactgttt cccacggtta acggagctgg aagtgctgac atcagcgtcg
     2341 ttgaaatatg caattgctga gcgtggttat cggttgggta gttatcttga tgtgtaagta
     2401 aggtggtaag gtcactcccc aggggacgtg accttatcca tatttacgcc ggaattttgt
     2461 caatcttagg aatgcgtgac cacacgcggt gttctgccat cagcgttaac agttcatcca
     2521 taaaactacc gtcggtgctg tcagcttcca caatcccttc ttcaccctgg tcagctacct
     2581 tgattgttgc tttaaacttg cgcgcgtctc ccgccagcgc aatcggttta aggtgtttgt
     2641 aggcttccat cagatagtag ttggcatcgc cgttgtcagc gatatccgcg atattgccgc
     2701 aagggacaat gaccgcatcg accgtcagcg aaggtgcacc ggcaaaggta gcggctatag
     2761 gcaacaccgt accgtcatcc gcagtcactt cacccattcg ggagtagagc agtttggcat
     2821 gaacgccttt ggccttcatg gccttgagaa tggccagaag gtctgccgat ctcacttcat
     2881 cattaagtaa aatcgctacc acgcgacctt tcacatcacc gtcaggaatg gcgtacaaac
     2941 ttaaggatgg atcctttttc agaccgttga cgtccggagg tggggtgata ttcagctggt
     3001 cgtcagtcag ttcgataccg agatttttcg ccaccgcctg ggccagagtg agatcaatat
     3061 gcgccagctg gtcaacaacg cgctcacgaa tatacggacg aacgactttg cttaactcaa
     3121 aactgaaacc atcgacaata tggcgctgct cgaatggcgt ctgacttagc cagaacagac
     3181 gcggatggga ataatattcg ccaaacgatg ggctgcgctc gcgaacttta ttgccttcca
     3241 cgcgctcttg gtatgattca aaaccgccgc gtttcggccc cggtggtgtt tcgcgcggcc
     3301 agttatcgtt gatcgagttc ggttcataat tcgccgggtt agtgtcgatc cccatacgat
     3361 gcatgccgtc acgctggaaa ttatggtaag ggcaggtcgg gcggttaatc ggaatctcat
     3421 ggaaattcgg cccaccaaga cgactgattt gtgtatcggt ataggagaac aaacgtccct
     3481 gcaacagggg atcgttggtg aagtccagac caggaacaat atgacctgga tggaatgctg
     3541 cttgttcgtt ttcggcaaag aagttatccg gattgcgatt gagcaccatt ttgccgacac
     3601 gctgaacggg caccagttct tccgggatca gtttggttgg atcgagaaga tcgaagtcga
     3661 acttgaattc atcttcttca ggaatcaact ggaagcccag ttcgtattcc ggaaaatcgc
     3721 ctgcttcaat ggcttcccac aactcgcggc ggtggaagtc cgggtcacgt ccggtgagtt
     3781 tttgtgcttc atcccaaacg agtgaggctt tacctgccag tggtttccag tggaaacgta
     3841 caaacgttgc cttcccttcg gcattaatca ggcggaaggt gtgaataccg aagccttcca
     3901 tggtgcggta actgcggggg atgccgcgat ccgacatcgc ccacatcacg ttgtgcagag
     3961 tttcaggttg cagagaaaca taatcccaga aagtatcgtg ggcactttgc ccttgtggaa
     4021 ttgcccagtg cggttctggt tttaccgcat gaacaaaatc ggggaattta tgcgcatcct
     4081 ggataaagaa gattggcgtg ttattgccaa cgaggtcaaa aataccctct tcagtataga
     4141 acttggtggc aaaaccacgg atatcacgca cggtatcggc agagccagcg ccaccctgaa
     4201 cggtagagaa cctaacaaat actggggtga ttttgttcgg atctgagagg aaatccgctt
     4261 tggtgatatc gcttaagctt ttatatggct ggaaataacc gtgagctgct gagccgcgtg
     4321 catgaacaat acgttccggg atgcgctcat ggtcaaagtg ggtgattttc tcgcgcagaa
     4381 taaaatcttc cagcagcgtt ggaccacggt taccggcacg cagtgagttt tgatcatcgg
     4441 cgatgcgcac gccctgatta gtggtcagcg cataattttc actgcctttg cgtacgtctt
     4501 ccagagaatt aagtttttcg ttacgtgtat caggggcttt caggctccca ggggcggtag
     4561 gttgtgcacc tggcggtgtt ggttcagccg ctggacgatg agatccgtcc tcaggtgcca
     4621 gtgagtccat ccccggtttc gcttcgctgg aatcgtgtag tggtgactgg tgctgatgtg
     4681 ggttcttttc gttatgttgc gacattgaac tcgtctcctt aatttattac tgaaagggcc
     4741 gctgttttgg tcaaaaaact ctctaactat agaccaccag ccagatcccg cttcggagac
     4801 tattctggaa acggtgttat gaaatacacg caaagcggga cgtacagggg ctaaatcggc
     4861 taaactacag tttttctaat ttttaatatg cgtttagtga agccagtaat gaagaaaccg
     4921 ctccgccagc aaaaccgcca gattattagc tatgtcccac gcacggaacc cgcgccgcca
     4981 gaacatgcga taaagatgga ttcgtttcgt gatgtctgga tgctgcgtgg caaatatgtt
     5041 gcgtttgtac tgatgggaga gtcatttctg cgctcaccgg cgtttactgt gcctgaatct
     5101 gcccaacgtt gggcaaatca gatccgccag gaaggggaag tgactgagta aggattacat
     5161 acctcccggt tttgccggga gttacaggac ctgaaattct gcatttcgtt gctacattca
     5221 ttcaaaccac ctgtaacaaa aagctgcctt tgacgagcta ttgctataaa taagagatct
     5281 tttttcttta attatttatt tattttaaaa tggttaagtt ttgctaatca tgatgagtta
     5341 tttttgatag ttcatcctgt ggcgtcgctc ttaaacttat attgtaaatg acttagattt
     5401 aaataagata ataattttgt taacaatatg aggcgggaga tgttttctac aaacttaata
     5461 cagagtaatt atggtgattt aaatattaaa agcctggctt tcgactcttt taaagaaagg
     5521 cttgagtcta ccatggcggc actcactttc tttatatcaa cgggtcaatg tgactgcgat
     5581 gaaatagcag agtcgaactt caatcgtatg attgcctata tgtctaacat taactatgat
     5641 gcctcaaagc taggagcgcc agcacttagt tttgatacgt atctgcagga caatgttaaa
     5701 tatagagtga taattaataa tctttatggc agtgagtttc ggataagaga tattaatgag
     5761 aattttgttg ggatggattt tacctgtgaa ttccgacccg aaaaaatgac gaatttgatc
     5821 aatataaaaa ataggttggt tattcattat ttaaggacta tatactatga aaaaaaatta
     5881 tatacatcct gccgggagca tttttgctgc gattcaaaaa aaatgagagt ctcctaaagt
     5941 ttccttcgat aatatcaatg cttaatatca atcttctttt taatccacta aatcttccgg
     6001 gaatgggaag tggtgtgctt gaagatatta tgagtattcc agatagttca cttagaaaac
     6061 gtctcgggta tgaggtttta agcttctcac tacaggcaca ttcattgagc caggaatgta
     6121 ttgataagtt ggacattttt ttcgctgacg atctgtttaa
//
LOCUS       DADLTY010000217         6058 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.219, whole genome shotgun sequence.
ACCESSION   DADLTY010000217 DADLTY010000000
VERSION     DADLTY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.219"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..143)
                     /locus_tag="JN334_003790"
     CDS             complement(<1..143)
                     /locus_tag="JN334_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HAY7439772.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="JN334_003791"
     CDS             379..651
                     /locus_tag="JN334_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HAY7439773.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="JN334_003792"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="JN334_003792"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HAY7439774.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1731
                     /locus_tag="JN334_003793"
     CDS             1111..1731
                     /locus_tag="JN334_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY7439775.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1740..3047)
                     /locus_tag="JN334_003794"
                     /pseudo
     CDS             complement(1740..3047)
                     /locus_tag="JN334_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
     gene            complement(3114..3767)
                     /locus_tag="JN334_003795"
     CDS             complement(3114..3767)
                     /locus_tag="JN334_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY7439776.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3767..5238)
                     /locus_tag="JN334_003796"
                     /pseudo
     CDS             complement(3767..5238)
                     /locus_tag="JN334_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5211..6058)
                     /locus_tag="JN334_003797"
                     /pseudo
     CDS             complement(5211..6058)
                     /locus_tag="JN334_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1427 a         1621 c         1599 g         1411 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggatc ttcggtgcgt tgtgctggat gacgaccttt
     1681 acgcgggtgt ggagtgggta tctgacgctg aagtcgctcc agcgtcagta attgcaatgt
     1741 tatttgctac tgcccgggcc gcgttcaccg gttgcaaccg gattttttgg atcgctgctc
     1801 cattcgtacc agccgccgtc atacacggaa acattcttcc aacccatggc gcgtgcgtac
     1861 ataaaggttt cggacgcgcg ccagccggtg ccgcagtaga atgaaacttg ctgatctggt
     1921 ttgatattcc atgctttcca catagcggta atatcatcgg cgctacgcat ggtgccatcc
     1981 gggttataga aatcttccat atgcgtcgag tcgctaccag cgtgtcccca acgtgctccg
     2041 gctatttcac cttttggttt aatatagctg taaccgctgg tcgtaccgat aaattctggc
     2101 cacgaacgaa tgctcaccag cgatgcatcc tggcgatgca gcagtccacg ggcctgttcc
     2161 atatcgagca tcaactgcgg ttgtgccggg atcttcacgc cgaaatccgg ttccgctttc
     2221 actttcggtg gcgttcctcg ctcaacaggc agtcccgcgt cggaccaggt ttgccagccg
     2281 ccatccagca gacgcacatc tttcacgcct gcgtagagca taatttgcgc cacacgcgct
     2341 ccagcgtata cgtcacgccc gtacagaatg accgtggtgt catggcgaat gccgtgtttt
     2401 gccagcatcg cttttagttg ttcatcagaa actttgttcc acagcggttc gctttccact
     2461 tcgttggtat cgatgtagtc agcgccagga atatggctga taaggtaaag cttaggagcg
     2521 ccctaggccg cttcaatgac tttccagtca ccgacaggtt tcgccgtaac ctctttacct
     2581 tgttgcaggt catgcagcca ttgcggataa accagctgct caaagtgcgg cagtttttgt
     2641 agacgggaag gttcgcttag cgcgtcactc agaatggaga tatgcgtaaa acctgctttt
     2701 tgcagtcgcg ttttgacggc gtcaacatct ttgccattac cgtacagcgc caccggggca
     2761 tcggctttca ggttatgttg cttgatccac gcgttgagct gttcggtgct cattttgtcc
     2821 agccagctag cagagaggtt taaggcggca ggttcatgac cagaagggcc atttaaggtt
     2881 tgtggccagc cgttataaaa cgcgctgggg cgagtatcta tcgctttgcc attttgttgc
     2941 tgaagttggt caagtgtaag aggcttcgcc agttcagcgg cccacgaaga agcgcaagcc
     3001 agccctaaag ccattgccag cgcggtcatt tgagaaacac gtttcatcga aaatcccgac
     3061 attgtagttt tgagtgcgcc attctgaact gacgcattga gaaaaacttt gcgttatcgc
     3121 agtttgttgt aattttctga ccactgcgcc atatccagaa cagaactatc agcaggaaca
     3181 tcctggagat cgtgcgttac ctgaacgacg gggatcgcca gggcgcgaac ttcgctgaac
     3241 acccactggc gaaaattatc gcgcagagcc acatcaagac ggctgaatgg ctcatccagg
     3301 agtaacgctt ttggctgggc gagaagggcg cgtagtagag caacgcgcgc tcgctgaccg
     3361 ccagacaaag tggcaggatc ctgatggaaa gctccgtcaa ggcctgaacg ctcaagtgca
     3421 tcattcacgg cattacgtcg ggcattcccc ttaagtgtcg ccggtagcgc cagcagtaaa
     3481 ttttgcccga cgctgaactg gtcaaataac agtgcatcct gaaaaagaat gccaatctga
     3541 cgctgtgcgg tgggtagcat gtcaatccgt tgctcattga gccatagctc acctgtacaa
     3601 gaaaactgtc cggccagtgc accaatcatc catgaaaaca gagtggattt tccacagcca
     3661 gacggcccca ttaacgtgac gatgtcacct ttatcaaccg taaagttaac gtttgtcagc
     3721 aagcggcttt ctggtaaacg tagcgaaaca tttttcacgc agagcattag cggattcctt
     3781 gtctgacata acctacccat tttgcgacta acgcggtcag ggcaaaaata ataagcggta
     3841 acaacagttg ccataacgcc tgggcggcga gaataccgtt gctgccgccg ctgcttaatg
     3901 ccaccgcctc agtggtgagc gtcggaaaac gccccgcgcc cagccacagc gtcggcatat
     3961 actgcgcaat actgactgaa aatcccaccg cgaaggcaat cagcacaggg cgcaacatga
     4021 gtgggcattt cacgtaaaag aagattttgg cccgcgacca gcccagtgtt tgcgcaatca
     4081 aaattaaccg tgaatcaatg cgctgccagg cgggttgcag gataaacagc atccacggca
     4141 ttacccacag cagatgcccc cagaccaccg ccgtccagct tccatccagg ttcagccata
     4201 gcgccagcgt gtactggccc gccaccagtg gcagagcggg caatagaatg ggcaaccata
     4261 gccacaactg gcgacgctgt gccccccatt ccagccacag cagtaacagg agcaaagcga
     4321 tgaatgttgc taccagcccc attgtcagac tgttgatgag tgcttcacta ttgatcgtcg
     4381 actgatccgc gaggatcgcc agcagaacca cacacagcac accggttaag ggtaaaaaac
     4441 tcgccagcgt agtgcctggt aataaaggtg tggcaggctt gcgaatgcca tctacgcggg
     4501 gaatagtgcg ccgccagctg cgccaaaacg taggcggcga gtaacagcat cagcagcaga
     4561 ctggcgagtg cgcctttcgt ttgttgatcc gcgtcgccct gagttaacca ctgccagcta
     4621 attactgcca gcgtcggtgg attacctggc ccaagaataa tcgccacatc cacgaccgaa
     4681 agcgaccagg caacaatcgc cagcatcgcc attgccagcg gctgtagccc agcgaatcca
     4741 gcacaatgac ctgctgcaac agccgttttt cgctcaatac tgccactaag atccacagca
     4801 gaaatgcgct ttctttcact gcaagggtga ggcccagacc gatccaaatc ggtccattgg
     4861 cggagtgaaa tacgggaaat agtcatatag cagccctccg tcagcaaaga gcagcagggc
     4921 gctggtggca aaagccacat ggggaatggc gagcagccac ggcagacggg cgcacatacg
     4981 ctgccatttc ggcccaggcc atagcgccac aatcaccagc agggcaatca acaatgcccc
     5041 gaccgccgcg atggttgtcg acaccagcgt tgccagtaat gcctgcggta actgtggatc
     5101 ggcaaataac gcctgccagc gtgtcaacga caacgcaggc gatatcagcg tgagagctgc
     5161 cgggatcagc ggtgcataaa tcaccgccac catcgcccac agcaaaaaga ttaatgcgta
     5221 ccgtaacggc gtaaccattc ttgttccagt gcattcaccc atcctgcgtg cggttcagcc
     5281 agtaccggcg gcagatcctg cggcattctt gattgcaata tttcgcgctg cccgtcaggc
     5341 agtttttgcg gatcgagaac agaaggatcg ccccagacaa cgggatctgc tttacgcagt
     5401 tgcgcatcgg gtgagagcag gaaattggca actaccttcg ccgcagcact ggcattcgcg
     5461 ttggctggaa tggtgacgaa atgcacgttg ccaatcatcc cctcgcgaaa gccaaaactg
     5521 taactgcttg ccggcaaatc gccgctggcg attttttgct gcgcatgtgc ggggttaaag
     5581 gtcagcgaca ggcgcaatgt gccggctttc agcagagcat ccatccgcgc gggcgaaggc
     5641 gggaaatcct ttccttcgcg ccacaaatac ggatgcagtg catcaagata ttgctacaag
     5701 ggcgcagtga cacgggcgaa agtcgcatcg tccggcgctt cttttaatgc ggcgggatct
     5761 ggcgtcagca taatcagcaa ctgttcaaga aacgccgtgc cggtaaagtc cggtgggcgc
     5821 ggataagtaa ccgtgccggg attggcttta gcaaactcca gtaaggcttg cggcgtttgt
     5881 ggtggctgtg gcgtaacatc gcggcgggcg ataaacgtca gttgtgcgcc gccccagggc
     5941 gattccgcac cttgtgtcgg cactgaaaaa tctccccgca ccggcagctg tgtgtcgaca
     6001 tagcgccagt tgggcagagt ctccgcccag cccgtttgta ataaattggc ctctttta
//
LOCUS       DADLTY010000218         6052 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.220, whole genome shotgun sequence.
ACCESSION   DADLTY010000218 DADLTY010000000
VERSION     DADLTY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.220"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            430..1338
                     /locus_tag="JN334_003798"
     CDS             430..1338
                     /locus_tag="JN334_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAY7439777.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="JN334_003799"
     CDS             complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="JN334_003799"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY7439778.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3653..4411
                     /locus_tag="JN334_003800"
     CDS             3653..4411
                     /locus_tag="JN334_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7439779.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4617..5537)
                     /gene="speB"
                     /locus_tag="JN334_003801"
     CDS             complement(4617..5537)
                     /gene="speB"
                     /locus_tag="JN334_003801"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAY7439780.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5673..>6052)
                     /locus_tag="JN334_003802"
     CDS             complement(5673..>6052)
                     /locus_tag="JN334_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439781.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1492 a         1575 c         1561 g         1424 t
ORIGIN      
        1 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
       61 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      121 aaagcaattg ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata
      181 acaacaatat gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa
      241 atcaacattt tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt
      301 cgaaaacata cttttctaat gaaatgtttt ttcgctatac ccctttatag attctgaatt
      361 ctcacctgtc ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg
      421 aagattgtca tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct
      481 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
      541 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg
      601 ctgggtttta agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct
      661 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta
      721 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact
      781 attgaagcat taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt
      841 ttcggccaat ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg
      901 caaaatgcca tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa
      961 aatattgatt tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc
     1021 gccattgcca cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta
     1081 actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagcaga agcggaaact
     1141 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
     1201 gaagcaatcc gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc
     1261 actgccgaac gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg
     1321 tttatctcta ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac
     1381 tgattacagc agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc
     1441 aaacagcagc tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt
     1501 caggccaaca tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt
     1561 aaccgctttc ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt
     1621 agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc
     1681 aaccgccagt tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca
     1741 gtctttcagc acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg
     1801 ctgcgccagg ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc
     1861 gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg
     1921 ggtcacgcgc agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag
     1981 accgatggag tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg
     2041 tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc
     2101 accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg
     2161 gatgtagtta cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt
     2221 agacggtgcc aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc
     2281 gatagcattc tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc
     2341 gataaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt
     2401 aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc
     2461 agattctttc gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat
     2521 ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc
     2581 cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat
     2641 ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg
     2701 tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc
     2761 gaaacgcatt gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc
     2821 gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac
     2881 ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc
     2941 gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat
     3001 accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt
     3061 ataacctact tccggatggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc
     3121 ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg
     3181 gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt
     3241 caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg
     3301 accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc
     3361 tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg
     3421 acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg
     3481 tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg
     3541 cgcaatctgc tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac
     3601 ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat
     3661 tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga
     3721 ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc
     3781 gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc
     3841 gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa
     3901 tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc
     3961 aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga
     4021 agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg
     4081 gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat
     4141 tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca
     4201 attctcccag cgccaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg
     4261 cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg
     4321 tcgtcaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg
     4381 cgatcgcatc agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat
     4441 aaggcgttca cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg
     4501 atgcgatgct ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt
     4561 tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac
     4621 tcgccctttt tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga
     4681 gcggtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg
     4741 ttgagatctt tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc
     4801 actggcgtac cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg
     4861 taaaccggca tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga
     4921 tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga
     4981 ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     5041 atagtgccgt ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt
     5101 accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag
     5161 tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc
     5221 tgcagctttt cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc
     5281 acgacgttca gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc
     5341 agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa
     5401 gtggccatat cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg
     5461 aagttcatcg gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga
     5521 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     5581 cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc
     5641 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     5701 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg
     5761 ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc
     5821 gaattggctg cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc agtatgttta
     5881 tctttgcgga atcctttaat cactaacgaa tctccggcat tcacaatata gccgttgtta
     5941 ttcagcgaac cttgcttgcc gttcagcaca tccaggccat cagccgtagc aacgatctcg
     6001 tagttggtgt tacgactgta attacggaca tataattgat aattcattcc ga
//
LOCUS       DADLTY010000219         5956 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.221, whole genome shotgun sequence.
ACCESSION   DADLTY010000219 DADLTY010000000
VERSION     DADLTY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.221"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            292..888
                     /gene="wrbA"
                     /locus_tag="JN334_003803"
     CDS             292..888
                     /gene="wrbA"
                     /locus_tag="JN334_003803"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY7439782.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            909..1136
                     /locus_tag="JN334_003804"
     CDS             909..1136
                     /locus_tag="JN334_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439783.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(1174..2415)
                     /gene="agp"
                     /locus_tag="JN334_003805"
     CDS             complement(1174..2415)
                     /gene="agp"
                     /locus_tag="JN334_003805"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HAY7439784.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(2708..3964)
                     /locus_tag="JN334_003806"
     CDS             complement(2708..3964)
                     /locus_tag="JN334_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HAY7439785.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            4225..5145
                     /gene="cbpA"
                     /locus_tag="JN334_003807"
     CDS             4225..5145
                     /gene="cbpA"
                     /locus_tag="JN334_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HAY7439786.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            5145..5450
                     /gene="cbpM"
                     /locus_tag="JN334_003808"
     CDS             5145..5450
                     /gene="cbpM"
                     /locus_tag="JN334_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HAY7439787.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(5543..>5956)
                     /gene="torD"
                     /locus_tag="JN334_003809"
     CDS             complement(5543..>5956)
                     /gene="torD"
                     /locus_tag="JN334_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HAY7439788.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         1597 a         1426 c         1345 g         1588 t
ORIGIN      
        1 ggactaagct tagtgaactg acgcggacag acccgcgttt ggtaatatcc tgcaacagct
       61 tcttagcgag aatagatatc atctccaata attatccata agccgctctt atgattaaaa
      121 aaacgcgatg agataaaaat gtgtcatttt gcgacaaaat tacgtgcttg tgaaagttgt
      181 tataaatcaa ataagtggtt gtgaaatttg cactctgaaa aggacgtctt atctttaaat
      241 aagtggtagc gaatcgctac ggaatagaga taacacgagg agtggttaga aatggctaaa
      301 gttctggtgc tttattattc catgtacgga catattgaaa cgatggcacg cgcagtcgct
      361 gagggtgcaa gcaaagtgga tggcgcagaa gttgtcgtta agcgtgtacc ggaaaccatg
      421 ccgccgcaat tatttgaaaa agcaggcggt aaaacgcaaa ctgcaccggt tgcaaccccg
      481 caagaactgg ccgattacga cgccattatt tttggtacac ctacccgctt tggcaacatg
      541 tccggtcaaa tgcgtacctt cctcgaccag acgggcggcc tgtgggcttc cggcgcacta
      601 tacggaaaac tggcgagcgt ctttagttcc accggtactg gcggcggtca ggaacaaacc
      661 atcacatcca cctggacgac ccttgcgcat cacggcatgg tgattgtccc cattggctac
      721 gcagcgcagg aattatttga cgtttcacag gttcgcggcg gtacgccgta cggcgcaacc
      781 accatcgcag gcggtgacgg ctcacgccag ccaagccagg aagaactgtc tattgctcgt
      841 tatcaagggg aatatgtcgc aggtctggca gttaaactta acggctaatc ttcaacagga
      901 ggatacgcat gccaactcaa gaagcgaaag ctcatcacgt cggtgaatgg gcatctctgc
      961 gcaatacgtc gccggaaata gccgaggcca ttttcgaagt cgccgggtac gacgaaaaaa
     1021 tggcagaaaa gatttgggaa gaaggtagtg atgaagtgtt agtcaaagcc tttgctaaga
     1081 ccgataaaga ttcgcttttt tggggcgaac agaccatcga acgtaaaaac gtttaaccag
     1141 cgactccccc gcttctcgcg ggggagtttt ctgttatttc accgcctcat tcaacacgct
     1201 atcaaactta tccatcgggc agaaaccgtt ggcatctatc gggcaaccgc ttaattccag
     1261 cgtcacgcgc tgcgcaggtg cctgcagggt taacgcatcg gcattacgta actgttccgc
     1321 actctggtac acatattcaa ttttcatcaa atcgcgattg gctttgctgt catgccaacg
     1381 ctggaaaacg attttgccgc caatcggcgt gcgttcgttc tggtcatgca actgatacgg
     1441 tttgaaatcc agcgccgtta acagagaggc tatgttggag tcgtgcccaa ctaacaccgt
     1501 gattttcgga gcactggcgc gatcggtgac cagggctttg tcgatataac tgaccagcgg
     1561 tttcgcaaca ttgcgcgcca cttccggtga ggtaaacaga ctgtcctggt aaccgttttt
     1621 cagcttcgac aataccttcc actgctggtc agatttgatt tctccccagg ccacctgatc
     1681 catcggaaaa ccttcgtagt attgcaaagt aaacgcatct accagcgagt tgccgacttt
     1741 cagcggcccg gaaacacctg gttcttgttg atacttggcg ctaaaggtat ttttgccatc
     1801 caccagcgaa cactgttgtt tctctttaca ggcaggggaa tctttatagt taacgatttt
     1861 ttccagtagc tggtagctgt cggtaagctg gagtttgctg agctctttct ccattgccgc
     1921 caccgcctgt tcactgaatg cggcggaatc atcggtgatc atcgggttaa aggttgggtc
     1981 catagtgccc attttttcct ggtgatgcac aggaatatca cacccaggga atgcaccagt
     2041 aataaagaac tgggcggtag cgacggtacg ttgcagacta ttggcatagg cataaacggt
     2101 gtccggcggc gggcattccc ccgatttcac tatcccctgc tctgccagcc attcacgcat
     2161 gtaatggccc atatacactt cgagcacgcc acctttggtg gtgagttgcc caccggggac
     2221 gtcccattcg ggccatttat tcggcgtcga ctgctccagc acactgccat tgttcgccag
     2281 cggcgcacgt aagttatggc ggctcatcat gagcacttgc tgtagctgat agccttccgg
     2341 tacggtttgt gcctgagcat ttgaagcgag taaaactatc cctgccacag ttgcggcgat
     2401 tagcgttttg ttcattcctg gcacctcttt tgttatcaat cattaaagag tgtgacagaa
     2461 atatgacttt tttccgggaa caggttcgca aaatggcttg ttgaacgcct gtttttctat
     2521 atacatcttc aaaaacaggc aaggtttgac gcaaactatg cctgattagc ccctgcctcg
     2581 cgtacgccgc tgataattgc acctgtggct aaacagaata tctgcggcgt taccaataca
     2641 gagacaatat ttaccaataa aaaagtgtga gactaataac aatggttttt agcatatgcc
     2701 tttgccatta agttgcataa cgatttaaat agtcaagttg cgcattcatt tccttatttt
     2761 ttaacttata agcctgagaa aaaagttcag gagggaggta aacatcataa tgattgccag
     2821 tgtttgttaa atatccatac tcaagatctt catctgttga aaaggattca tttcccaagg
     2881 agattgatgc cacattacta ttaacaataa ataatgagca aatgctatct gaaagaacag
     2941 caagaaaatt cttcaccgct ttaatcctat ccgacgtgtc ggtatctaaa gcaagcaaat
     3001 tcttgttatc attaaatata aactcgggta aatgcagtat gcattttttg tcattcaagc
     3061 aactatcaat gaattgtttt ttaaaaaaca gaaagctcca tgacataaaa ctacccacac
     3121 cctgaatttt gttgtcagat tgcagatcaa cgccaaataa tgtctttaca tcttccctga
     3181 gattatagaa tgccgtatca acaagagcct cgatcgtttg tttatcaaca attttaataa
     3241 actcttcgta tacttgcttt tgcgtcttat ggctgcccag cgcccatgaa tactcatgtt
     3301 cttttaaaac cagtatggct cttaacagac aatatccatc gccatctact ggagcgtatt
     3361 ttaatccata tgttttatta atttcactct gaataaaaga tatgccagac aaatcaatac
     3421 cagtatctaa atcagatatt cccggcatat ttatctgttc tggctggata ctcatatttt
     3481 cattatctaa acaatactca gaaaaaacat catcacacgc attaatgttt tcaatatatt
     3541 ccataaagcc ataccaataa taacctttaa aatctgtgat agactctgaa cctctgcccg
     3601 gcaaggacac tctaacaata tcaacatctt gaacatattg tatagtatat gtaatacctc
     3661 ctgaaacccc tgaataaatt tgtgtatcat tagtacgcaa tgttctcata aataatgata
     3721 gatcattatc attctgcatt tcctcaaatt ctttttctag tgattcacat tccagacatg
     3781 gagtatcctc ttcatttact ttacgtctga gttcattgta caaccgcata aaacacacca
     3841 gtgtgttttc aaaaagcgtg attgaatatg tttgattttt ttcgtactca ttttttatat
     3901 cgttccaggc ctgtttcaaa taattatttg cagtgcaact aattccatgt atattactac
     3961 ccatatatag cgtctataaa atttaataaa taatgacgcc ctagttaaac ttaaagtgcc
     4021 tggttcaact atcaaaaatc gctcaccctt tttcacctgt ttaaaatatg ttcagcaacc
     4081 catcttgatg gcgacctcct ctccgcgatg atttcaataa catattctgt gttggcatat
     4141 gaaattttga ggattaccct acacttatag gagttacctt acaggggttc cttcaatttg
     4201 tgttgattta cgcgagataa cgctatggaa ttaaaggatt attacgccat catgggcgtg
     4261 aaaccgacgg acgatctcaa gacaatcaag accgcctatc gtcgactggc ccgtaaatac
     4321 catcctgatg tcagcaaaga accggatgcc gaagcccgct tcaaagaggt cgctgaagcc
     4381 tgggaagtgt taagtgatga acaacgtcgc gctgagtatg atcagatgtg gcaacatcgc
     4441 aacgatccgc aatttaaccg tcagttccat catggcgacg gtcagagttt taatgccgaa
     4501 gattttgacg atatcttctc gtcaattttc ggtcagcatg cccgccagag ccgtcaacgc
     4561 cccgccgcac gtggccacga tattgaaatc gaagtggcgg tattcctcga agaaacgctt
     4621 actgagcata agcgtaccat cagctataac ctgccggttt ataacgcctt tggcatgatc
     4681 gaacaggaaa tcccgaaaac gctgaatgtg aagatcccgg caggcgtcgg caatggtcaa
     4741 cgcatccgcc tgaaaggcca ggggacgccg ggcgaaaacg gcggtccaaa tggcgatttg
     4801 tggctggtga ttcatattgc gccacatccg ctgttcgata ttgtcggcca ggatctggaa
     4861 attgtggtgc cggttagccc gtgggaagcg gcgctgggta ctaaagtcac cgttccaaca
     4921 ctgaaagaaa gcattttgct gactatcccg ccaggcagcc aggccgggca acgattgcgc
     4981 gttaaaggca aaggtctggt gagcaaaaaa cagaccggcg atctgtatgc ggtactgaaa
     5041 atcgtgatgc cgccgaaacc ggatgaaaac actgccgcgc tgtggcagca actggcagac
     5101 gcccagtcgt cttttgatcc acgtaaagat tgggggaaag cataatggct aatgttacgg
     5161 tgacttttac tattaccgaa ttttgcctgc ataccggcat ctctgaagag gagttgaatg
     5221 aaattgtcgg tttgggggtg gttgaaccgc gtgagatcca ggaaacaacc tgggtatttg
     5281 acgaccatgc cgccattgtg gtgcaacgcg cggtacgcct gcgtcatgaa ctggctctgg
     5341 actggccggg gatcgcggtg gcgctgacgt taatggatga tattgcgcac ctgaagcagg
     5401 aaaaccgcct gctacgccag cggctttccc ggtttgtagc tcatccgtga tgtgtgtttg
     5461 ccggatgtga tgacgcatcc ggcaactgcg ccataatgcc tgatgcgacg ctaaacgcgt
     5521 cttttcaggc gcataaacga cgttatctgt tttggtggtc gcactccact aacaccagca
     5581 ataactggct tagtgccgcg taaaaaccaa agctgtcata ctgtcgacaa cgcgcagcaa
     5641 actctggtaa ccattgccgc agcgccgtca gtgttttttg ccgcaaactg tcgattcttc
     5701 gcgcaggaac ggtcccctct cccagcgaaa aatgcagata actcaacaac tcgagataga
     5761 tcgccagatg atctgccggt tcgttgaaat tgccactggt ttccatccct gcctcaacta
     5821 acaagcgttt aatctcttgc tcgtcctgtt tgtaggccga tgcatacggc agcgccgctt
     5881 gtttgtcggt catcagaaac aggccgcaaa agtcagcggc cagttccaga cgggcatcgt
     5941 cacgtactgt cagcgt
//
LOCUS       DADLTY010000220         5936 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.222, whole genome shotgun sequence.
ACCESSION   DADLTY010000220 DADLTY010000000
VERSION     DADLTY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.222"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            659..1450
                     /locus_tag="JN334_003810"
     CDS             659..1450
                     /locus_tag="JN334_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439789.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            1836..2042
                     /locus_tag="JN334_003811"
     CDS             1836..2042
                     /locus_tag="JN334_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY7439790.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            2128..2700
                     /locus_tag="JN334_003812"
     CDS             2128..2700
                     /locus_tag="JN334_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439791.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2702..2986)
                     /locus_tag="JN334_003813"
     CDS             complement(2702..2986)
                     /locus_tag="JN334_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439792.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            2957..3100
                     /locus_tag="JN334_003814"
                     /pseudo
     CDS             2957..3100
                     /locus_tag="JN334_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            4269..5288
                     /locus_tag="JN334_003815"
     CDS             4269..5288
                     /locus_tag="JN334_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7439793.1"
                     /translation="MNNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIR
                     NVNLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
     gene            5398..>5936
                     /locus_tag="JN334_003816"
     CDS             5398..>5936
                     /locus_tag="JN334_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HAY7439794.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIR"
BASE COUNT         1881 a         1321 c         1167 g         1567 t
ORIGIN      
        1 ctcttgacta taaagatgat gacgggatca gaacagttat taatccatac aacaaaatac
       61 agaaaacaac agaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaataacaga cattacttaa
      121 caaagaacat tacagtcagt gtgtacatta cagatttata ttcaggtgtt gcgggactac
      181 attacagcag agaggggtaa ggacagagtt acaagtacta ctagagagaa tatcactgca
      241 cagatacttc tatgagtata tctttatgag agatccgtct acaggattgg gttataaggt
      301 gtatttacaa ggtataatca caggatcatt tacatgacca tacttctgta aataatacta
      361 attaacctta attatttttt tatggtacat gaactacctg tagctgtaac gataaacaaa
      421 tcacccaaaa tgaaaaatac tggttatcac agttcagaga actcgtatga gtgtccccct
      481 gagcaatgct tcattttctg tgattctgtg tgatagagtt atactgagta cagtggcagt
      541 aacactgctt tccatcactt gaggataaaa ttcattaaaa aattaattgc atgaattaac
      601 aggttttttt atgaaatgtt atgcatctca accaatatga ttaacagaaa acttcatcat
      661 gacaacacgt ataacgttca gtatgaccag tgatgagacg ctgcgaattg tggatgagta
      721 ctgtcatacc cacaaacttt cgcgctctaa ggtaatcaac gcattactca gcgccacagc
      781 acctgtcctt aatgatatta actgttatta tcaacttgca ggcgaactgc agtcacgctt
      841 attaaatgga gtgtatcaac gggatcttcc tcgcaaacgc aatgtcgtca gtgcagagaa
      901 gtattgtctg gaaatctggg aaaacaaact tttcaccaga agaatcctgg agtttgacag
      961 ttcaaatggt gttctgtacg cgctgaaaca taaaagacac tatcgccggg ataagatgat
     1021 tggaagggtt gaaagtcggt gtatcaaaga catttgcgaa taccagatgc agctatccgg
     1081 ggagaagacg aaatatgcct gttttattta tatcgaaaga acaatataca atcacgataa
     1141 tccatctgat aaaatacctg taaaagcggc tgttggtaat gcagtcatcc tgctggcaaa
     1201 ggatgtgata tataacgagt acttttttga tttgcggcag tcattttttg tgagcgttac
     1261 agatctgatg gtctctggcg caaaaggtat tccggagaca cagacatatc cggacgttta
     1321 ttgctggatc cctctttttt ccataaattc cggcgtgctc attacccctg tatataaaat
     1381 tgaccctctg aaacccgtaa cggtaaaaaa accggataag atcactgttg tttgtaatta
     1441 cagagaataa tttcagcctt ctaccttcac cgcgacagtg tttacctctg ctttatcttc
     1501 cgttcagtaa atacaaacca ccgctctggc aggtacaggg cgtggtttat ctccgatact
     1561 cccctgtgca gtaataatca tcactgaaaa tactgatccc gtcacggcaa aacgccatcc
     1621 catattccgg caacacaaaa tcatcttccg tttataccgg attcactgac aaaacagaca
     1681 acctgtattg cctgtacaca acagtttctc tcctggcagg aatattcccc tactcatttt
     1741 ttacgtttct ccccggttgt tatgccggtg aaggtggtgc gtcgttttca tcaccaacgc
     1801 cggttgagat taacaacatc cggaggaaca ttctcatgaa taccccagtt tcactgatgg
     1861 atgaccagat ggtcgacatg tcgtttatca ctcagctgac cggcctgacc gataagtggt
     1921 tttataaact catcaagcat ggagcctttc cgccccccat caaactgggc cgtagctccc
     1981 gctggctgaa aagcgaagtg gaagcctggc tgcaggcgcg tattgcacag tcccgtccgt
     2041 aattcctgac cgttatccgt tccaccgcag caaaggcatg atccggcccc cggaggcatt
     2101 ttgctgcgcg ctaaaggaga gaatattatg caacaacttg aaaacaggtg tgacctgctg
     2161 ctcatccagc atcagaaatg gatgacaagc gtgacgaggc ttatcgtcgc tcatggaatg
     2221 gggtctccgc atcttcatgg ttatcaccgt ctgaccctgg cacacttttt tctgccggaa
     2281 aaaggctccg tcatttcggt ggccccccag ggactctatc aggtcgtcaa cccgggaaca
     2341 ccaccgttca ttcctgccat tcaggaaggg ttaatgacca gcattcagac ccatgaaatc
     2401 atgctgctta cacattttaa tctgggcggc gttttactca gtgagctaca tcgtctgggt
     2461 gaaaaccggc tggcaaacag gctgaacagc ctgctccggc gtttcgacga tcgtgatctc
     2521 taccacacgc tgatatggct gtgctggtat gatctgatgt gtgcccactc catgcagccc
     2581 tggactgaag agttaaagca taaatcacat gcagagcttg aaaactgggc tgtggcgcgc
     2641 aaacgggaaa agcgcgagct ggaactgatg attgacgagt acctgttata cgcctgctga
     2701 ttcataacgt tcccggggtt ccttcctcat cgttacacac agccggacgg ctatctatgg
     2761 ctgtccgcca aatgaccatc gccgcatttc gttccgctga aaacggtacg tagtcagtga
     2821 taccggaatt tccggagagg gccgcaggta tattgcttct gtgcaattga tgcttattta
     2881 cattggatgt agttatttct gcatccaatg ctatcggaaa gagattaacc agcaaatggt
     2941 tcctcaaatc gtattgatat ttacccggcg tattgagcac caccatctga cactccgttc
     3001 ccgcatcaaa cgactggacc gaaaaacgat ttacttttca cgtgctgtgg aaattcacga
     3061 aaaaattatt gggtcattca tcgaaaaaca tatgttctga tgactggagt catcctccag
     3121 aaatatacgt caactgaaca atatcgattt tttatgggat tctatacact tttccatatc
     3181 cctctgaaaa tcagtcatca gctcaccaac acctcttctg aaacttgtat taaacttatt
     3241 gcttaaatag atgatataac atctcttcca ttgcactgaa tcggcgaaaa ctgccgtttc
     3301 aataatctca acaatattat ttatcggcta actttatcag taaacaatgg aatgtcacac
     3361 tgatgaaatg aacattcaga gtaagatgat aaaattcata taaacgccat tgctgaatat
     3421 tatttatttc atacagataa acgataaaat acaaaagggt atatttctca atgtaacagt
     3481 caattattca tctccactgg cgagcacggt cagcacacaa acaaagttag tcctcctgct
     3541 taatcattgc gtatcagaaa ctaaaaaaga aaaaattctg cagacaactt aacaatcttc
     3601 atcacatttc ctgaagacag acatatccac aaacggtagt cagcgttgaa ttaacaagtc
     3661 ttacagagct atacgtagct tataaagcca ctcaatgttc atcatcaccc gactacttat
     3721 ctggaatacc accagtataa actcatcaat aaaaacacct gttgttaaaa taagaatact
     3781 gtcagcagta aattattaaa acaacatctg tccacactat ctgttctttt aacgccaacg
     3841 gcagcgaaaa tagtgcatca cagccttctt ttttctttcg ccaccactca gacaaacaca
     3901 acactcacac ccacaccaca aaacagaatc aaaacataaa attaacaata acagtcaaaa
     3961 ggaaaacaca gaatattgag gtggactgtc accaggacag cgcatacgac gcatgtgatc
     4021 agcacatccc gttcttcgct ggcatcccca aaatgcagaa caaacaatca taacattatg
     4081 aatataaaag aaaatatgat ttttcaccat gaaaaaaaca tcaatgtatc tctgtataaa
     4141 aataatgata acatgacata aacatttaaa aacataaata aacagcaaca aaaacatttg
     4201 ataatcactt agtgattaaa tacaattaag acgattttgt tataagagag gcaccatgga
     4261 gtgagataat gaataacgaa cagatgctta tcaaatgcag agatccccgt aaatgtcggt
     4321 tttatgccga atatctgaat cacaaatacc ctaatgaatt ccctcacttt gcgcctgaca
     4381 acccacaaaa ttgtatcagg aatgttaatt tatttttaaa ggaaaagtat tgtaatgttt
     4441 cacctccccc ctttggtact tttggtacta ctgaagaaga agcactcaat aacgaaatta
     4501 atgagcttga aattattgcc gggcaggcaa tactgcctga agagagtttt ctctggttga
     4561 aaactgacga acttgcgact ttctttacct ggactactat ttatctgtcg aaggaagacc
     4621 atacaaaatt cccgaaaata aaccataatt catcaggaat aaaaaacgga attgaagtca
     4681 ggaaaatacg aacagttacc gatgaaacgt taaacactta caaaaaaagt aaccttccgg
     4741 ataatccatc atcccataac gaacgaataa aagtaatcat ttcatatttt gactcctgcc
     4801 attctttaat taataaaaat gcaaaaagca tatacctcga ggaactcagg caggcgtggc
     4861 ttaaaaagaa aagcgcttgt caaaagttta agtggctcct tcctgacgat gatgaaatgt
     4921 gtcagtggtt ctggacaaga ctgaaacaaa aacaggatga aaaacatcca gtacataacc
     4981 cagtgacccg gtggttcata ccatcttcaa cgaaagaata ttatcttgcc tctcttgtcg
     5041 cctttgcttt atggaaagaa gcgcctgata ccattgaact gttcagaaca cgaataaatc
     5101 atgcctggca tcagaaaaaa caaaaggaca aatatcggag tgatggtaaa aaggccatta
     5161 atatacatat aagaagcaat gtgaaagaaa tgcttgatga gctcagaact gtttacggtc
     5221 tgagaacagg agagtttttt gaaatgctga ttacagagaa atatcttgaa cacaagcgaa
     5281 ggaagtaatt cattacctgt tacattctaa ataatctcag acagatatta tctccgtgaa
     5341 tcccccgcca cctttccggt gcgcggggtt ttgtcttttt tcaccgggaa tacatgtatg
     5401 aatccgtctg atgccattga ggcaattgaa aaaccgctct cctccctgcc ttactcactt
     5461 tcccgtcaca tcctggaaca tctgcgcaaa ctcaccagtc acgaacccgt gattggcatt
     5521 atgggtaaaa gcggggccgg taaatcttca ctctgtaatg cactgtttca gggggaggtc
     5581 acaccggtca gtgatgttca cgccggcacc cgggaagtgc agcgcttccg tctgagtggt
     5641 cacggtcaca gcatggttat cactgacctg cccggggtgg gcgagagccg ggacagggat
     5701 gcagagtatg aagccctgta ccgtgacatt ctgcctgaac tggacctggt gctgtggctg
     5761 attaaagccg atgaccgggc cctgtctgtg gatgagtatt tctggcgaca catcctgcag
     5821 tgcggacatc agcaggtgct gtttgtggtg acgcaggctg acaaaacgga accctgccat
     5881 gaatgggata tggcaggtat tcagccttct cccgcacagg aacagaacat tcgtga
//
LOCUS       DADLTY010000221         5890 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.223, whole genome shotgun sequence.
ACCESSION   DADLTY010000221 DADLTY010000000
VERSION     DADLTY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.223"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..756
                     /locus_tag="JN334_003817"
     CDS             <1..756
                     /locus_tag="JN334_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAY7439795.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
     gene            complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="JN334_003818"
     CDS             complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="JN334_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HAY7439796.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(1792..1995)
                     /locus_tag="JN334_003819"
     CDS             complement(1792..1995)
                     /locus_tag="JN334_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY7439797.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="JN334_003820"
     CDS             complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="JN334_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HAY7439798.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            complement(4588..5100)
                     /locus_tag="JN334_003821"
     CDS             complement(4588..5100)
                     /locus_tag="JN334_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HAY7439799.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            complement(5118..5804)
                     /locus_tag="JN334_003822"
     CDS             complement(5118..5804)
                     /locus_tag="JN334_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase"
                     /protein_id="HAY7439800.1"
                     /translation="MEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRGVQ
                     ADLGEVVAWRNTFWALSDSMCSEATPWVYGAYLPDHAALQTYRVLAPMAYAKIKNIIE
                     RNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEFGG
                     RHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHNND
                     DINMLDKLLK"
BASE COUNT         1474 a         1524 c         1516 g         1376 t
ORIGIN      
        1 tatactaaga ttttagagaa tgattttgaa cacccttcaa tacgacgaaa aatagcatcg
       61 ctaagtaatt taggggtaga tgagtggatt cctccggtta tggcattcat gaatcgaatg
      121 gctcgtacag aagattttaa tttagatgat ttttctcaat ttattaccgc ctttgagaag
      181 gtatatatgc atgggtggtt aaaaaaacaa attaaaagcc aaagagaaat ggtttgttat
      241 tctgctctgg tagcaataaa taatgatatg ccttttgatt ctgtgataaa tcagataaac
      301 caacatgcag ataattcagg ttttatagct gctctggatg aagatttata tgagcctcgt
      361 ccgaatcaag tgaacttaat aaaagcaatt ttgcttcgct tagatatgga gcaacaagat
      421 gagagcgtga ttaaaaccta tacggggaga atcacaattg agcatattct tcctcaagca
      481 ttagttaatg aatactggat taatagattc caacctcaag agcatgtcta ctggctgcat
      541 aaaataggta atttaacact cataagtggt tccaaaaatt ctgaaacaca acactatgat
      601 tttatcaaaa agaaatctat ttatgagaaa cttaatagta aaagctcttt ttatttaaca
      661 aaagatgttt gtaattcttc agaatgggga ttggctgaac tgaaaatgag acatgaaaaa
      721 atgaaaacgc aattgaagaa gctttggttg gtctgacata tctaaagagt tccactcgta
      781 ataatgttgc ggtggaactc tcaaaaagtt gatctttccc gaacttactt cctcaatccc
      841 gcaaacgccg cccgaatctc ttcctccggc aactgaatcc cgataaacac cattgtgcta
      901 tgcggttttt catcgcccca cggcctgtcc cagtcggcgc tgtagaggcg ctggacgccc
      961 tggaacagca ggcggttagg ttcgccgtca atccacagca tccctttgta acgcagcagt
     1021 ttatccgccg attccagcag caggttttcc atcacgcggg aaacttcgct gatatctacc
     1081 gggtaatcca gttccaccac aattgacgaa atatcgtttt gtttatccgc gataaagtgg
     1141 aaacgcggtt tggtgctgac gacgttctct tccagcataa aaccgttggt gttgaacagc
     1201 aggcccaggt cgatgtcgcc gtgggtgacg gtgtagaccg gtgcgcgggc gttgatgcgc
     1261 gccaggcgtt cacgcagttt ctctgcttcg cctgcgacgt cggttttagt cagcagaata
     1321 cggtcggcgt agcccacctg ggactgggcg atggtgaact ggttcatctg ctcatcggca
     1381 tgtaccgcat ccaccagcgc aatcacgccg tccagcaggt agagctggca taaaatctca
     1441 tgggagaaaa aggtctgaat aatcgggccg ggatcggcca tgccggtgca ttcaatcacc
     1501 agacgatcga actgaatatt gcccttgtcg agattatcca gcaggtcgag tagtgcgtcc
     1561 tccagctcgt tggagcgcga acagcagatg cagccgttgg tcagcgtttt gatctgcgtg
     1621 gcgcggtcgc caatcagttg gtcatcaacg gagacttcgc cgaattcgtt ttcaatcacg
     1681 gcaatcttgt agccgtgttg ttcgttaaga atatggcgca gcagggtagt tttgcccgcg
     1741 cctaaaaaac cggtgagtag ggtaactgca atcgggttca tgctctctcc attaacaaca
     1801 gcgcataccg cctttaccat cgccgccgta gcgcgcgttc tggcgttcgc ggaagaattc
     1861 ttcatagctc atgtacggct tgtcgggatg gttggtcttc atatgctcga cgtagttgtc
     1921 gtagtctgga ataccaatca acatcttcgc cgcctggccg agatattttt ttgcctgtcc
     1981 taagttacca aacatagttc actctgataa gaacaaagcc ccgccgaagc ggggctaaac
     2041 acggttagtg gtgcgaagag atcttcacgc cgccttccgg gattggaacg tacggtgttt
     2101 ctttgtcagt acgtttgtcg ctgttacgca ccgcaagcca ggttttgaaa ccgtagaaga
     2161 tgatgctgta caccacaatc aggaacagaa tactcaggcc tgcgttggtg tagttgttca
     2221 caacgatgtg gttcatgttg gcaatctgct gcgccgtcag gtcagtaccg ttagcaatct
     2281 tctctttgta ctggcttgcc atgtagaaga agccttccat ctgcgggttg gtgctgaaca
     2341 gtttcagacc cagcgcccag gtggtgcaga taagcagcca tacagccgga acaacagtta
     2401 cccagatgta ttgggtgcgc ttcatcttaa tcagcacaac ggtgcccagt accagcgcta
     2461 cggctgccag catctggttg gagataccga acagcggcca caggctctta acgccgccca
     2521 gcggatcgac cacgccctga tacagcaggt agccccacag acccacacag cccgcagtac
     2581 cgatgatacc ggcaaccaga gagtcggttt ttttcaggaa cgggatgaag ttacccagca
     2641 ggtcttgcag cataaagcgg ccagaacggg tacccgcatc cagcgcggtc aggatgaaca
     2701 gggcttcgaa cagaataccg aagtgatacc agaagcccat gtcagccatc ggcagcactt
     2761 tgtggaacac gtgagcgata cctaccgcca gcgtcggcgc accacctgca cggttcagga
     2821 cagaaggctc accaatgtct ttcgcggttt gcaggatctg ctctggcgaa atcacgaagc
     2881 cccaggagct gacggtcgct gccgcgtgtg cggtaacgtc tttcagctgc gccatgatga
     2941 tcggcgcgtt ctcgccaccc atttcatgca ggttaggcat ggtgatgcca aggccagcag
     3001 gcggggtgtt catcgcgaag taaagacccg gttcgatgat ggacgctgca accagcgcca
     3061 taatcgccac gaaggactcc atcagcattg cgccgtagcc aatgaaacgc gcgtcggttt
     3121 cgttagccag cagtttcggc gtcgtaccgg aagagatcag cgcgtggaag ccagataccg
     3181 caccacaggc gatggtgatg aacaggaacg ggaacagagc gcctttccac agcgggccag
     3241 taccgtcaat gtactgggtc atcgcaggca ttttcagttc cgggttcagc accacgatac
     3301 ccagcgccag gccgacgata acgccgattt tcaggaaggt tgccagatag tcgcgcggtg
     3361 cgaggatcag ccacaccggc agcagtgcgg aaacaaacgc atagccaatc agcgcgaagg
     3421 taatggtggt gtctttaaag gtcagtgccg gaccccagta cggatcgtga gcaatcacgc
     3481 caccgaagta gatagaggca accagcagca cgataccaat aacagagact tcacccacac
     3541 gccccggacg gataaagcgc atgtagatac ccataaacag cgcaatcggt acggttgagc
     3601 aaacggtgaa gacaccccac ggactttcgg ccagggcttt aaccacaatc agcgccagaa
     3661 cggcgaggat gatgatcatg attaagaaac agccaaacag cgcgatagtc cccggtactg
     3721 gccccatctc ttctttgatc atctcaccga gagatgcgcc gttacggcga gaggagataa
     3781 acagcaccat aaagtcctgt accgcaccgg ccagcactac ccccgccagc agccacagcg
     3841 tgccaggcag gtagcccatc tgcgcggcga gaaccggtcc caccagcgga ccagcaccgg
     3901 cgatagcggc gaagtggtga ccaaacaaca cgtaacggtt ggtcggaacg tagttcagac
     3961 cgtcgttgtt aataaccgca ggcgtcgcgc gcgtggggtc gagtttcatc accttctggg
     4021 cgatgtacag actgtagtag cgatacgcca ccagatacac cgacacagag gcgaccacga
     4081 tccacagggc gctgacgtgc tccccccgac gtaatgcaac taccgcgagg cagaatgcac
     4141 cgatgattcc gagaatcacc cagggtatgt gcttgaatag ttttttcgta tccatagtaa
     4201 aacctggcat tttactcgtc gtcttttggc ttacaggtgc ggcagctgcg ttcgttcacc
     4261 tctgacactt acttttgtaa gctcccgggg attcactcac ttgctgcctt cctgcaaacc
     4321 aaaatccata gagtaatttg ttaatcatag gccgaagccg ttctaggttt gcgttgcgtt
     4381 tgaggaggta aattgaccgc tatcctgcag tgatgttgcc agagatacgt gcgcgtaaag
     4441 taaggtaaat aactgagtgg ttattttagg gatgtaagcg gtcaggaatg gcggtgagcg
     4501 gttgggcaag aattattcat cgggtagata tgtgattgag atcacaataa aaatgcccct
     4561 tcgacgaatg cgtgaagggg ctggagctta aatcgcagct tccatttcca gcatcaccgg
     4621 atggaaacgg cgtttaaagt aaataagtcc gtgaccttct gcactgagga tgatgttttt
     4681 aatctccacc agatacacca gatgtgtgcc aatcgcctgc acatcgcgga tctctccttc
     4741 aagactggcc agcgaacctt ttagcaccgg ctgcgccagc ggaccttttt gccagcatga
     4801 gaggctaaaa cgctcttcca tcgccatgcc tgtcatgccc gcgaagtggc gcgccatcag
     4861 ttcctgctca tggttgagga cgttgacgca caacttgccg ttgccctgaa aaaccgggtt
     4921 catcgcactg ttggcgttaa tgcacaccat cagcgacggt ggtgtatccg tgaccgagca
     4981 gacagccgtt gccgtaatcc cgcattgtcc ggcgtcgccc tcggtggtga taatatttac
     5041 cgctgccgac aggctggcca tcgcgtcatg aaagcgcagg cgttgttcat ctaattgcat
     5101 cttaacctcc tgctgcgtta tttcagcagc ttatccagca tgttgatatc gtcgttgttg
     5161 tgcaggtgcg gcacagtcca gccgttctgg tcgtattccg acaggcagcg atcaaccatc
     5221 gccatcatct tgtccatatt gccggagctt tgtgcctggc gcagacattg caggcgaatc
     5281 tcatcctggc taccggagta gttgatttca tacagctcgt gacgaccacc aaattcgctg
     5341 ccaatggcgt cccacatcag tttgaggatc ttgatgcgct ggacgtgatc cataccgttc
     5401 gaaccgcgca catacttcgc cagatactgg tcgatctgcg gattgttcag gtcacgggca
     5461 ctggaaggga ggtagatcag gccactggta acgttgcgtt ctataatgtt tttgatcttc
     5521 gcgtaggcca ttggtgccag tacgcgatag gtttgcagtg cggcatgatc cggtaaataa
     5581 gccccgtaga cccacggcgt cgcttctgaa cacatcgagt cactcaatgc ccagaaggtg
     5641 ttgcgccacg ccaccacttc accgagatcg gcctgcacac cacggaactc cagggtgccg
     5701 gtacattcga gtgatttttt cagcagtgcc gtaatgaagt cgagtttcac tgccaggcgc
     5761 acacaggctt gcagcggata catacgggcg aaaccgcctt ccatcgtcca gcgacggcag
     5821 cgatcaaaat cgcggtagat cagcacgttt tcccatggga tcagtacgtt atccatcacc
     5881 agaatcgcat
//
LOCUS       DADLTY010000222         5889 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.224, whole genome shotgun sequence.
ACCESSION   DADLTY010000222 DADLTY010000000
VERSION     DADLTY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.224"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            50..244
                     /locus_tag="JN334_003823"
     CDS             50..244
                     /locus_tag="JN334_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HAY7439801.1"
                     /translation="MLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVR
                     EILEEIEASILSESVNAKAG"
     gene            511..1437
                     /gene="tauA"
                     /locus_tag="JN334_003824"
     CDS             511..1437
                     /gene="tauA"
                     /locus_tag="JN334_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7439802.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="JN334_003825"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="JN334_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HAY7439803.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="JN334_003826"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="JN334_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HAY7439804.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="JN334_003827"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="JN334_003827"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HAY7439805.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFDHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="JN334_003828"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="JN334_003828"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAY7439806.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5493..5864
                     /locus_tag="JN334_003829"
     CDS             5493..5864
                     /locus_tag="JN334_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HAY7439807.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTD"
BASE COUNT         1401 a         1453 c         1598 g         1437 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgacca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt
     5041 tgtcgcgatg ttgacaagaa gagaatgaaa gagagaccag gacatctttg taatagaaat
     5101 agcaataatt tcatattcac tgaaaatatt tttaatcttt atttatagct cttggttatt
     5161 attttttggc gtttggttgt gctgctataa attgaataat tcaaacattg ttgtatattg
     5221 ataagatatt tatctggttt gtatttttga tatttataac gcgtatgcat caaaaataac
     5281 attatctgca acatactgaa atatcgtcct tttatcaatt tgatgcgtgt ttttctccat
     5341 aaattatata tgtccacatt cggacttagg ggaaagaata attgaaccat tcgtctgtaa
     5401 cgcagcataa tcgttagcgc gaaacataat atgttttcta tgcattgata attgatggat
     5461 caacttatta cgtccctgag gagggatgac aaatgcactc ctggaaaaag aaacttgtag
     5521 tatcacaatt agcattggct tgcactctgg ctatcacctc tcaggctaat gcagcgacca
     5581 acgatatttc tggtcaaact tacaatactt tccatcacta caacgacgcc acctatgctg
     5641 atgacgttta ctatgatggt tatgtaggct ggaacaacta tgccgctgat agctattaca
     5701 acggcgatat ctacccggtc attaataacg ctaccgttaa cggcgtgatt tctacctact
     5761 atctggacga cggtatttct accaatacca acgccaatag tctgacaatc aaaaacagca
     5821 ctattcacgg tatgatttat tccgagtgca tgacaactga ttagactggc cccctgaatc
     5881 tccagacaa
//
LOCUS       DADLTY010000223         5822 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.225, whole genome shotgun sequence.
ACCESSION   DADLTY010000223 DADLTY010000000
VERSION     DADLTY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.225"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(1..393)
                     /locus_tag="JN334_003830"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="JN334_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="JN334_003831"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="JN334_003831"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HAY7439808.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="JN334_003832"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="JN334_003832"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HAY7439809.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1804 a         1321 c          964 g         1733 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg taaattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cc
//
LOCUS       DADLTY010000224         5805 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.226, whole genome shotgun sequence.
ACCESSION   DADLTY010000224 DADLTY010000000
VERSION     DADLTY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.226"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="JN334_003833"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="JN334_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HAY7439810.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="JN334_003834"
     CDS             578..1027
                     /locus_tag="JN334_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7439811.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1261)
                     /locus_tag="JN334_003835"
     CDS             complement(1028..1261)
                     /locus_tag="JN334_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HAY7439812.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1347..1520)
                     /locus_tag="JN334_003836"
     CDS             complement(1347..1520)
                     /locus_tag="JN334_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAY7439813.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1715..1810
                     /gene="yncL"
                     /locus_tag="JN334_003837"
     CDS             1715..1810
                     /gene="yncL"
                     /locus_tag="JN334_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HAY7439814.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2212..3021)
                     /locus_tag="JN334_003838"
     CDS             complement(2212..3021)
                     /locus_tag="JN334_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HAY7439815.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3231..4655)
                     /gene="patD"
                     /locus_tag="JN334_003839"
     CDS             complement(3231..4655)
                     /gene="patD"
                     /locus_tag="JN334_003839"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HAY7439816.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVSRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4677..5471)
                     /locus_tag="JN334_003840"
     CDS             complement(4677..5471)
                     /locus_tag="JN334_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7439817.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5461..>5805)
                     /locus_tag="JN334_003841"
     CDS             complement(5461..>5805)
                     /locus_tag="JN334_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY7439818.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1522 a         1473 c         1287 g         1523 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt gtatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagtcg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcattggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg cgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcgac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggcgcgct tccatatctt
     1081 ctttatgctt gcgaccgtga tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc
     1141 gatcttcccg acttaactcc gcgtcatcgc tcagggcgat taataattcg ttcatatggg
     1201 caatgacgct ctcttcaatc gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca
     1261 tactttctcc ttagccactt gtgaaattta tcgattaatc caaatgatat aagtctgcgt
     1321 ggtgccgtca agccagtgag taagatttaa aacagtgaaa acaacagcgc caccgggaaa
     1381 ctcatcggcc atgtggcccc caccagaaag gcgcttaaga aacggatgcg tttcttatcg
     1441 tgagaaagaa accaggtgat aagaaatgcg attgctgcca taaccgcata aaaaacaagc
     1501 atatgttgat agagaaacat gtacgggcgt ccagccatat gaataaaacg cgcgcaaata
     1561 tgcgccattt tttgacgtgc atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca
     1621 gcaacttgcc cttatccatc ttcagaatga gtattattgc ggattttctt aacccgtact
     1681 atacacatag gggcaatgat aaaaggtggc aaaaatgaat gtttccagta gaactgtagt
     1741 actgataaat gtctttgctg ctgttggttt gtttactctt atctctatga gatttggctg
     1801 gtttatttga tgtagtgatt ttgcacgcag cacgtactgt cagagaaatc aactctctga
     1861 cagtacgcaa gatcttctca tactctccaa gtttgcaaca ttatccttaa tatttacttc
     1921 tttttgctgg catctacatt gaaatgagta gaaaacatcc ccctactgtc gtctatacaa
     1981 acagcttact gattcaaacg gaatcttgtt ttcacatttt tctatcacga atactttata
     2041 cgcgcgggag tctataaatg ctttatactt tcaaggaata acgtaaatat aaaaagatgg
     2101 cgtaaagtat atgcatggca ggatgtttgc aacctccttg atgacgctgc cccattgaaa
     2161 ttgcaaaccc cgccctggtt atcttgccca tttatcatgc aaaacagata attataggcc
     2221 tctgaatacc tcagaaagat tgaatttttt atagtccctc tcttgaatat ttccatttga
     2281 tatcaatcga ttcatctctt caacaaaata atttatatca tcacacttat tattatccgg
     2341 aagatctgag tagttataat tccaccattt tattctcaac agttcatcaa taacatttga
     2401 atcaaaccta aatctgatta tttttgcagg aactcctgca actatggcat atgggggcac
     2461 atctttggta acaacggaat tggcagcaat aacagcacca tcacctattg cgatgccacc
     2521 tttcaataca acatcatttc ctatccagac atcatttcct ataatcggat tttcaggttt
     2581 ggtaatgaat ggtttcaatg tccagctacg ttcaaattca ctcatagcaa acttatcaaa
     2641 ttcaccgtta taagtcatca tgtgagtagt aaaacgattc aaaggatgat ttccacccat
     2701 tatttttaca tttgatgcaa tactacaaaa tcgacctata cgtatattcc ctgaaaaatg
     2761 gcaggtcgga aatgaaaaag cgccgacact ggagaaattt ttcacaggca ttgttgaata
     2821 ttcttcagca ataacattat cttcaattgt tatcttgtca ccttttgaga aaagaaataa
     2881 attcctttct ttatacgact tcttcccctt atgatttaaa aagattttat tatccataca
     2941 aaactcaaag tgttttttcg tccatctgaa ggtaagggtt ttatttttat cttccgtgaa
     3001 tttgtacaat tgaactttca ttgttaatac ttctacttat aaagtattag tttaataacg
     3061 atactctaaa gccttatgct aatcaactgt gacaggatga aaaatcgcca tcaaacattt
     3121 agcctaattt ttacgccagt tattaatgta atcattctaa tacttaaccg ccattaaact
     3181 cagaacctaa aacgatagtg gccgtcctat aacagccact aacatctaac ttaatgttta
     3241 accatgacgt ggcggacgac ggtgtaatcc tccagcccat aaagtgacat atccttgccg
     3301 taaccagaaa gtttctgccc accgtgcggc atttcactta ccagcatgaa atgggtattg
     3361 acccaggtac aaccatattg cagccgtgcg ctgacgcgat gcgccctgct cacatctttc
     3421 gtccataccg aagatgcaag tccgtactgg ctgtcattcg cccagttcac cacctgttct
     3481 tcgttgtcga agggcgtaac actcactact ggaccaaata cctctttttg cacgatggca
     3541 tcgtcctgta atgcgccagc cagcagcgtc ggcgcatagt aatagccatt acccttgcgc
     3601 ttttcaccgc cagtgatcac tttgatgtgc cctgtcgctt tcgcctcttc tactgccttg
     3661 ctgacgcgtt cgagatgcgc cagcgagctt aaaggtccaa gctccgtaga ctcgtcatcc
     3721 ggcgcaccag attttaacgt tgccaccgca gcacccagtt tttccaccag cgtatcgtaa
     3781 atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag
     3841 ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact
     3901 ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg
     3961 atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg
     4021 gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg
     4081 aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt
     4141 ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc
     4201 agcggataat tccacggtgc gatagaagcc acgaccccca acggatcgcg acggatcatc
     4261 gaagtatgac cttcaagata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc
     4321 gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc
     4381 ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca
     4441 tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca
     4501 aatgctgcat ctgccgcacg cacagcagca tcgacctgct ctgcggatac ctcggcaatc
     4561 tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta
     4621 acccgttctc cgttaatcag taacttatgt tgcatagctt attcctgtat cagtggttat
     4681 tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc
     4741 aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga
     4801 ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt
     4861 tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc
     4921 ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca
     4981 accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg
     5041 caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta
     5101 aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat
     5161 aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc
     5221 gtccctaaca ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg
     5281 gcatcaagaa tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc
     5341 ggtggaaaac taaacgccgc atcttcagtg ttaaacgcat aggcggcgat tatcaggatg
     5401 ggaaaatgta ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt
     5461 tcagagtgca tcgaacgctc ccagacgttt cacgaacgcc aggtacagtg cgatgagaat
     5521 aatcggcacc agggtgaatg ccgccgccat cggcatattg ccaatcgccc cctgctggga
     5581 ataaaccata ttgccgataa aatatcctgg aggaccaacc agctgcggga cgataaaatc
     5641 gcccagtgtg agtgagaagg taaagataga gccagcggca atacccggga ttgccagcgg
     5701 cagcaccaca tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa
     5761 caatgacggc ggcaaacgct caagcgccgc ctgaacgggc aggat
//
LOCUS       DADLTY010000225         5786 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.227, whole genome shotgun sequence.
ACCESSION   DADLTY010000225 DADLTY010000000
VERSION     DADLTY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.227"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..971)
                     /locus_tag="JN334_003842"
     CDS             complement(<1..971)
                     /locus_tag="JN334_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7439819.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSSPDYH
                     GPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
     gene            1063..1149
                     /locus_tag="JN334_003843"
                     /pseudo
     CDS             1063..1149
                     /locus_tag="JN334_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1159..1239)
                     /locus_tag="JN334_003844"
                     /pseudo
     CDS             complement(1159..1239)
                     /locus_tag="JN334_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1554..2030)
                     /locus_tag="JN334_003845"
     CDS             complement(1554..2030)
                     /locus_tag="JN334_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HAY7439820.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(2089..3378)
                     /gene="bioA"
                     /locus_tag="JN334_003846"
     CDS             complement(2089..3378)
                     /gene="bioA"
                     /locus_tag="JN334_003846"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAY7439821.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3465..4505
                     /gene="bioB"
                     /locus_tag="JN334_003847"
     CDS             3465..4505
                     /gene="bioB"
                     /locus_tag="JN334_003847"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAY7439822.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4502..5656
                     /gene="bioF"
                     /locus_tag="JN334_003848"
     CDS             4502..5656
                     /gene="bioF"
                     /locus_tag="JN334_003848"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAY7439823.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5643..>5786
                     /locus_tag="JN334_003849"
     CDS             5643..>5786
                     /locus_tag="JN334_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="methyltransferase; acyl carrier protein involved in
                     an unidentified step in the synthesis of pimeloyl-CoA, a
                     biotin precursor; member of the bio operon (bioABFCD); in
                     Escherichia coli, bioC-null mutants require biotin for
                     growth; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAY7439824.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLD"
BASE COUNT         1408 a         1435 c         1597 g         1346 t
ORIGIN      
        1 tgctcttcat gttcaaaagc atgccatatc tgtgatacat cagattgttt gttttccggg
       61 aaccatgctg tcacggcatc agccaggggg cgatggagtg tattctgttg tccgtcactc
      121 atggagaagt aaatccgtgg gccgtggtag tccggcgaag aggttaatct ttgcagggct
      181 tgcagagtgc gagtcgacaa tggattgtta taaatattaa catctgcata aatgtgcaga
      241 ttcatgatac tgtctggcaa tgtgttcagc tgattgttag ggatatatag tgattgcaga
      301 cgttgtggaa gttcgggcag tctgtgtagt tggttttccc caacgtttaa aagcctcaga
      361 ttttgaggaa ggggcggtag ctcttgtaat ctgttatcaa aagcacttag ttcctgtagt
      421 tcacatggta attctggtaa ggcagtaagc tggttgttgc caacagaaag tttttgtaga
      481 gccgggggaa gatcaggtag cgtctccagg ctattgagaa gggctgagag tgactgcaag
      541 gaagatggaa gagaaggcaa acatgttaaa actctgttaa gtgaaacatc aagagcgacc
      601 aggtgaggcg gaagagccgg gagcctgctc agtctgttat cgctggcttt aagtacagtt
      661 aaggacggag ggagttctgg cagagagcgt agctcattgc tagagatgtt aagttcttgt
      721 atgtgcgggg gcaggtatgg gagagagcgt aatctgagta aacttaaatt gagggctggc
      781 tcttgaaaag ccagacatat tttcagtaat cgaactgcct gtgttcggtc ttctgttgca
      841 gcaccttcct tggcccagtt atcccagatg cggtgataat ccgtgatatt ttgctcttct
      901 acggataaac gggatataca tcctaaacca caaaaggagc ggaaaaaaga aggattgtgg
      961 gcaggtttca tttattattg ttcctcattg tttatatatt aatatattcg tgcaaatgga
     1021 gtgaggattc tttaaaaaaa cgcgcctgta aatagttaag aacaggaaga actgttgggg
     1081 atgtggctgg ccgaaaatga aggggtgaag ttctggctca atgtgctgac tgaactgaaa
     1141 aaacgcggtt ggtcgcccta ccttttcatc aagccagtag tctgcccacc acagcatcat
     1201 ttctctgcgc ttatcgagat actgagcatg gttgtaaatt cagctaaccc agtgcgttga
     1261 caaaaaatta gcgcaagagg acaaaaaatc accttgcgct aatgctctgt atcaggtcac
     1321 taataccatc taagtggttg attcatagtg actggatatg ttgtgttttg cagtattatg
     1381 cagtctattt tttaagctaa atgtattata atgcattgat attaatgatt tttactgttt
     1441 ctcggtcagc ttttttatac taacttgagc gaaacgggaa ggtaaaaaga caaaaagttg
     1501 tttttaatac ctttaagtga taccagatgg cattgcgcca tctggcagag tgattaacta
     1561 aacatcgcgg taatcgatgc gcttgccaga gagtggaaat gaacgttaaa cccgaccatc
     1621 gcgccgctgg ctccttcatc gacatcaata cgctctacat ccagcgcgtg aacggtaaaa
     1681 atgtagcgat gggtttcgcc tttcggcggc gctgcgccat cgtatccggt tttaccaaaa
     1741 tcggtacgcg tctgcaaaac gccgtctggc attgctacca gaccagagcc aaacccttgc
     1801 ggtaatacgc gggtatcagc gggtaaatta acaactaccc agtgccacca gccggagccg
     1861 gttggcgcat ccgggtcgta gcaggtgaca acaaaacttt tcgttcccgc aggaacatca
     1921 tcccacgcca gatgcggtga aatattatcg ccatcgtaac ccatgccgtt aaagacatga
     1981 cgatgcggca acttatcgcc atcgcgcaga tcgttactga tgagtttcat gaaccctcct
     2041 ttcttgtttg cagaaagtgt agccagaaac cctcacgctg acttcccgtt attggcaaaa
     2101 aaatgtttca tcctgtaccg cgcggttaac cgctgcggtc agacgctgca actgttgcgg
     2161 gagaataata tagggcggca tcaggtaaat cagtttgcca aaaggccgga tccatacccc
     2221 ctgttcgaca aagaattttt gcagcgccgc catattcacc ggacgagtgg tttcgaccac
     2281 accgattgcc cccagtacgc gcacatcggc aaccatttcg gcatcacggg ctggtgccag
     2341 ttgctcgcgc agctgcactt caatagccgc cacttgctgc tgccattcgc cggattcgat
     2401 aatcgctagg ctggcgtttg ctgccgcgca ggccagcgga ttgcccataa aagttggccc
     2461 atgcataaag cagccggctt cgccgttact gatggtttct gcaacctcgc gcgtggtaag
     2521 tgtggcggaa agggtcattg tgccgccggt taaggcttta ccgaggcaca aaatgtccgg
     2581 cgcgatttct gcatattcac aggcaaacag tttgccggta cgaccaaatc cggtggcgat
     2641 ctcgtcggca atcagcaaga taccttcgcg atcgcacatt ttgcggattc gttttaacca
     2701 ttccggatgg tacatgcgca tcccgcctgc gccctggaca atcggctcaa tgatcaccgc
     2761 tgcgatttca tgacgatgcg ccgccatcag gcgggcaaaa ccaaccatat cgcgttcatc
     2821 ccattcacca tccatgcggc tttgcggggc gggagcaaac aagttttctg gcagatagcc
     2881 tttccacagg ctgtgcattg agttgtccgg atcgcacacc gacatcgcgc caaaggtatc
     2941 gccatgataa ccattgcgga aggtcagaaa acgctggcgc gcttcgcctt tggcttgcca
     3001 gtactgcaac gccattttca tcgccacttc caccgctacg gaaccggagt ccgcgagaaa
     3061 aacgcactcc agcggttgcg gcgtcattgc caccagtttg cggcatagct cgatggcggg
     3121 cgcatgggtg ataccgccaa acatcacatg cgacatggca tcaatttgcg tcttcatcgc
     3181 cgcattaagc tgcgggtgat tgtagccgtg gatcgccgcc caccaggacg acataccgtc
     3241 aaccaggcgt ctgccgtcag acaaaattag ctcgcaacct tcggcgctcg ccaccggata
     3301 aaccggcaga ggggaggtca tggatgtgta tgggtgccag atatggcgtt ggtcaaaggc
     3361 aagatcgtcc gttgtcataa tcgacttgta aaccaaattg aaaagattta ggtttacaag
     3421 cctacaccga attaacaaca aaaaacacgt tttggagaag ccccatggct caccgcccac
     3481 gctggacatt gtcgcaagtc acagaattat ttgaaaaacc gttgctggat ctgctgtttg
     3541 aagcgcagca ggtgcatcgc cagcatttcg aacctcgtca ggtgcaggtc agcacgttgc
     3601 tgtcgattaa gaccggagct tgtccggaag attgcaaata ctgcccgcaa agctcgcgct
     3661 acaaaaccgg gctggaagcc gagcggttga tggaagttga acaggtgctg gagtcggcgc
     3721 gcaaagcgaa agcggcagga tcgacgcgct tctgtatggg cgcggcatgg aagaatcccc
     3781 acgaacgcga tatgccgtac ctggaacaaa tggtgcaggg ggtaaaagcg atggggctgg
     3841 aggcgtgtat gacgctgggc acgttgagtg aatctcaggc gcagcgcctc gcgaacgccg
     3901 ggctggatta ctacaaccac aacctggaca cctcgccgga gttttacggc aatatcatca
     3961 ccacacgcac ttatcaggaa cgcctcgata cgctggaaaa agtgcgcgat gccgggatca
     4021 aagtctgctc aggtggcatt gtgggcttag gcgaaacggt aaaagatcgc gccggattat
     4081 tgctgcaact ggcaaacctg ccgacgccgc cggaaagcgt accaatcaac atgctggtga
     4141 aggtgaaagg cacgccgctt gccgataacg atgacgttga tgcctttgat tttattcgca
     4201 ccattgccgt agcgcggatc atgatgccga cctcttacgt gcgcctttct gccggacgcg
     4261 agcagatgaa cgaacagact caggcgatgt gctttatggc aggcgcaaac tcgattttct
     4321 acggttgcaa actgctgacc acgccgaatc cggaagaaga taaagacctg caactgttcc
     4381 gcaaactggg gctaaatccg cagcaaactg ccgtgctggc aggggataac gaacaacagc
     4441 aacgtcttgg acaggcgctg atgaccccgg acaccgacga atattacaac gcggcagcat
     4501 tatgagctgg caggagaaaa tcaacgcggc gctcgatgcg cggcgtgctg ccgatgccct
     4561 gcgtcgccgt tatccggtgg cgcaaggagc cggacgctgg ctggtggcgg atgatcgcca
     4621 gtatctgaac ttttccagta acgattattt aggtttaagc catcatccgc aaattatccg
     4681 tgcctggaag cagggggcgg agcaatttgg catcggtagc ggcggctccg gtcacgtcag
     4741 cggttatagc gtggtgcatc aggcgctgga agaagaactg gccgagtggc ttggctattc
     4801 gcgggcgctg ctgtttatct ctggttttgc cgcgaatcag gcagtcattg ccgcgatgat
     4861 ggcgaaagag gaccgtattg ttgccgaccg gcttagccat gcctcattgc tggaggctgc
     4921 cagtttaagc ccgtcgcagc ttcgccgttt tactcataac gatgtcgctc atctggcgcg
     4981 actgcttgct tccccctgtc cggggcagca actggtggtg acagaaggcg tgttcagcat
     5041 ggacggcgat agcgcgccac tggcggaaat ccagcaggta acgcaacagc acaatggctg
     5101 gttgatggtc gatgatgccc acggcacggg cgttatcggg gagcaggggc gcggcagctg
     5161 ctggctgcaa aaggtaaaac cagaattgct ggtagtgact tttggcaaag gatttggcgt
     5221 cagtggggct gcggtgcttt gctcaagtac ggtggcggat tatctgctgc aattcgcccg
     5281 ccaccttatc tacagcacca gtatgccgcc cgctcaggcg caggcattac gtgcgtcgct
     5341 ggcggtcatt cgccgtgatg agggggatgc acggcgcgaa aaactggtgt cactcattgc
     5401 gcgttttcgt gccggagtac aggatttgcc gtttacgctg gctgattcat gcagcgccat
     5461 ccagccattg atcgtcggtg ataacagccg tgcgttacaa ctggcagaaa aactgcgcca
     5521 gcaaggctgc tgggtcacgg cgattcgccc gccaaccgta cccgctggta ctgcgcgact
     5581 gcgcttaacg ctaaccgctg cgcatgaaat gcaggatatc gaccgtctgc tggaggtgct
     5641 gcatggcaac ggttaataaa caagccattg cagcggcatt tggtcgggca gccgcacact
     5701 atgagcaaca tgcagatcta cagcgccaga gtgctgacgc cttactggca atgcttccac
     5761 agcgtaaata cacccacgta ctggac
//
LOCUS       DADLTY010000226         5696 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.229, whole genome shotgun sequence.
ACCESSION   DADLTY010000226 DADLTY010000000
VERSION     DADLTY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.229"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..170)
                     /locus_tag="JN334_003850"
     CDS             complement(<1..170)
                     /locus_tag="JN334_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439825.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="JN334_003851"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="JN334_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAY7439826.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="JN334_003852"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="JN334_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4413)
                     /locus_tag="JN334_003853"
     CDS             complement(3616..4413)
                     /locus_tag="JN334_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7439827.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRINVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLP
                     GSCIYLDAGTTTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5481
                     /locus_tag="JN334_003854"
                     /pseudo
     CDS             4579..5481
                     /locus_tag="JN334_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            5478..>5696
                     /locus_tag="JN334_003855"
     CDS             5478..>5696
                     /locus_tag="JN334_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HAY7439828.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1410 a         1468 c         1478 g         1340 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtggtcaa tgcggcctag
     4801 gttaaacagg tgctgtttgg tgaaacaggc gttgcacaac atctgaaacc cggtacggca
     4861 gtaatggttt cttccactat cgctagtgct gatgcgcaag aaattgctac cgctctggct
     4921 ggattcgatc tggaaatgct ggatgcgcca gtttctggtg gtgcagtaaa agccgctaac
     4981 ggtgaaatga ctgtcatggc ctccggtagc gatattgcct ttgaacgact ggtacccgtg
     5041 ctggaagccg ttgccggaaa agtttatcgc ataggtgcag aaccgggact aggttcgacc
     5101 gtaaaaatta ttcaccagtt gttagcgggc gtacatattg ctgccggagc cgaagcgatg
     5161 gcacttgcag cccgtgcggg gatcccgctg gatgtgatgt atgacgtcgt gaccaatgcc
     5221 gccggaaatt cctggatgtt cgaaaaccgg atgcgtcatg tggtggatgg cgattacacc
     5281 ccgcattcag ccgtcgatat ttttgttaag gatcttggtc tggttgccga tacagccaaa
     5341 gccctgcact tcccgctgcc attggcctca acagcattga atatgttcac cagcgccagt
     5401 aacgcgggtt acgggaaaga agacgatagc gcagttatca agattttctc tggcatcact
     5461 ctaccgggag cgaaatcatg atcaagattg gcgttatcgc cgatgatttt accggcgcga
     5521 cggatatcgc cagttttctg gtggaaaacg gtctaccaac ggtacaaatt aacggtgttc
     5581 caacaggtaa aatgccggaa gcaatcgacg cactggtgat cagcctgaaa acgcgctcct
     5641 gtccggtggt tgaagccaca cagcaatcgc tggcggctct gagctggttg caacag
//
LOCUS       DADLTY010000227         5667 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.230, whole genome shotgun sequence.
ACCESSION   DADLTY010000227 DADLTY010000000
VERSION     DADLTY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.230"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..242
                     /locus_tag="JN334_003856"
     CDS             <1..242
                     /locus_tag="JN334_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439829.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(239..1180)
                     /locus_tag="JN334_003857"
     CDS             complement(239..1180)
                     /locus_tag="JN334_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HAY7439830.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1325..1681)
                     /locus_tag="JN334_003858"
     CDS             complement(1325..1681)
                     /locus_tag="JN334_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439831.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDLH"
     gene            complement(1685..2905)
                     /locus_tag="JN334_003859"
     CDS             complement(1685..2905)
                     /locus_tag="JN334_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HAY7439832.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2909..3652)
                     /locus_tag="JN334_003860"
     CDS             complement(2909..3652)
                     /locus_tag="JN334_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAY7439833.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3543..5009)
                     /locus_tag="JN334_003861"
     CDS             complement(3543..5009)
                     /locus_tag="JN334_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HAY7439834.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5009..5242)
                     /locus_tag="JN334_003862"
     CDS             complement(5009..5242)
                     /locus_tag="JN334_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAY7439835.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5230..5649)
                     /locus_tag="JN334_003863"
     CDS             complement(5230..5649)
                     /locus_tag="JN334_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terL-like domain protein"
                     /protein_id="HAY7439836.1"
                     /translation="MADSGTDKCANVYRHGSVVFWADEWKAKEDELLKSCQRTYQAAL
                     EREADIVYDSIGVGASAGAKFSEINADRKSENAYARRVNYQRFNAGAGVHEPDDEYNG
                     IPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1211 a         1601 c         1420 g         1435 t
ORIGIN      
        1 tctttggcat tttttacctc gttacgcgcg tctgtggctt tgccaacgga tacttccgcg
       61 tgcgcacgag tgaaccagtg cgtggcaacg tcttcctcca caacatgacg gcctttaaca
      121 aactcgcgcc gagaaccgtc gggaagcgtg agcacaaacg gggtatgtac gtgtattact
      181 gcatcatttt ttgccatcgg gtcatcctta atggccccgc cagggggcca tgtggctgtt
      241 aaatgccatc aacgtacgaa atggtttctt tgtacactgg ctcgactgca cccagcttgc
      301 cgtagtaagt gacgatctga tacagaccgc gatactgcac cggcacgctc tgaagcggaa
      361 ccagcgggta gcggacgtat tttttatcgt tggtgtacgc aactatgcga tccttattcc
      421 ccacaccacg gcctttcagc catttaaccg cgcggatatt cagcggaaca ccgttctggt
      481 gatagctgat ggtgttggtc tgaaggttcg tcaacaggga ctggttacct gcagatgaaa
      541 cgatgatgct ggacaacaga gcaaactgct caggcgggat cagcaaatca cgcggaacca
      601 cagagtaacc agaagcggcc cacgcatcag acagcacctg gttaatgctt gcgcggattt
      661 cgtccggtgt tgaggttgcc cacgttttgg cagcgttgtt gacaggcacg ccgtccaggg
      721 taacaaggcc tttcaggttt aatgcggaat cgccaacata tacctgttca tcgttatcca
      781 tctgccattt cagttgcata ccgtcatact tctgcgtatc aatcgggcgg ccgacctgct
      841 gagcagcctg caattctatg accgtccagc caagttccat cccccacagg ttcagcgggt
      901 taccggattt gccgatatcc acgttcacgc cagcaatagc ggttgagtct ttgcctaccc
      961 agttttttcc attcggattt gcgccagtac ccgcagcggc gaagctggta ttcgtccagc
     1021 tggaaatgtc atctgcgatg gaggcgtctt cacgcagttg aatatcgcgg ctccaggtgt
     1081 accccaccag tggcaggttc agcgtctggt cgagtctctc cagctccccg atgagaaagg
     1141 caccagagct gtcaacggtt gcctgatcaa aagtaatcat tcgtctgttc cttaaatctt
     1201 ccaggaaatt tctgcattgc cgtcagcatc accggcacct gtgaattcag cgttggtcag
     1261 caccacgttt ttgccactga ctgacgtgga catgaatcca cccagaggca ctttgatgga
     1321 ttcatcagtg gagatcgcct tttttgatag tgctggcatc aaaatcagaa ccgagattaa
     1381 cggtcacgta gccacgcttc atggcgtcgc ccgggaagtt cttgcctgtt cccacctggc
     1441 gaaccatgtc cggctgcgac gtggtcggat aagggcgcac gtagatcccc ttcaccttgt
     1501 ctgcggtatc accatctgcc agcggcacga aaaaaccgtc atcatcgtat ttaccagcca
     1561 ggccataggc agcgaaggcg ttatcggatt taaggaccac cggttcgacg gttaagtcct
     1621 gcgggcgaga gacagccccg gcaatgccaa caggcatccg gtacagaaat acattattca
     1681 ttttttaccc tttacggttt gcccagaatt cagcgttttg tttgttcagg gaagcgatac
     1741 tggtcatgcc catgtttagg cgctgtgcat cgccggtggt ggcgcgggtg tttcgccctt
     1801 tggcaacctc agacacggca ttaaacgcca tgtcgaccga ttgtttcggt aatttgcgga
     1861 tatccgcatc accgactatc tggcgaacca gcgttttgtc agcagaagcc agaacctcgc
     1921 gtttgaacgc ggtcggtttc atcttacggc tcagatcgat acccggaacg ataacttcgg
     1981 cacgccaggc tgagtcacca gtaatcgtgg tttcctcttc atcgtcctcg ccgtcaccgg
     2041 tcggattatc gtcaggctta ttatcgttat cgcccgtggc atttctttcc agcttagcca
     2101 gcagggcttt cagtaatgtt ttgaggtcat catcactgtc gccggttgga tctccgccca
     2161 tctctggtgc tttgtccggt agtggttgct gcggggacag gttgatgttg agattaacgc
     2221 cctgcggcaa atccccctca tctcctgtaa ccgatgcggg agccgactcc accagttcgt
     2281 tcatggtgtc ggcatctcct gtcttgatgg ccgcacgcat gcggttccac cagtttttct
     2341 tttgatttgc cattgtgtct ctgtctccaa ttgcacaacg atttccggct ctgcctttag
     2401 ggacaagagc cacatggttt ccggtaatat cgacctgctc ggctttacct ggctcggtct
     2461 gctcgtactc cgcgtcatag ccgcacgaca cttcgcgcag gccatcttcg ataagctgaa
     2521 tggcgctttc gtctttgacg ataaggtcag ccagcatcaa atcagactgc tcacccgtcc
     2581 cgcgccggac attctggagg tgcccgacag caagcacttt ccagttctcg ggatttacca
     2641 gccgcacatt cccgttttca tcttcaggat gcaggatcgt gatgctcatc ccttcgaatg
     2701 aggcaagcgt ggccggatgg aatacctgct caggagaacg cgtgacgact atttcaacga
     2761 acttatcggg tttcagtttt ggcaggtcat cagcaccata gagctgctta cctgttcgtc
     2821 ctatcggcac gtctttgcac agcaacgagc cgtcagccag gcggtatcgg gtttccccga
     2881 gcctggtctc aaaaaagtat ttcatgggtt atgctgccct tttcattgcg aatggaacgc
     2941 gaacaaacac gatctcttta tagcagcggc agtttggcag ctcgcccgcg tggccggtca
     3001 tgccgtccaa agttggaggt gaatcccagc ggacgaattt acctttcatg tgatcgtggg
     3061 aatctcggac atcgccatca tcggctgtac gccaaatata accatccgag ccaatagcta
     3121 tcgctcgggc catatccagc gcgcccgttg ctcttcccag ttccgttcgg gcaatcagat
     3181 cggccctaga cttagcaaca tcgccggtag atgcaatctc tttagcaaac tgctccgccc
     3241 gaccaccggt gacaacagcc tctatcgctt tgttctggat gtcgtacacc ctgtctgcgg
     3301 cctcgagagg cagagatttg atgtacttga cctgttcggc gatgatggat tgcatcacct
     3361 ggcccacagg agcgctttcc acaagattgc ggagctcgcg actgatgttc ttgctgtgtt
     3421 gccgccaaac tttctcgttc tgccgggtta ggtccgcagt aaagttttcc gcgacctttg
     3481 tcgcccaggg ggtgatgatt tcactgtaac gctccagcgc cgcaataatt tccgtgatac
     3541 tgtcatttga accatcgtag cgaccattta cgatgtctcc gaccgccctc gctatcctgc
     3601 gtaggctggt tcgataccgg atttccgcct gacggctcct gcggttcgtc atcaggttcg
     3661 ccgatgccgg gcggcgcttc atcttcggca ttctcgatgt cctcgttggt aatggatgcc
     3721 cctatgccgg ttacgtcaga attttctcgc aaatcagtca tagcggcttt cagtgtcatc
     3781 agaccatcac ccagcgccgt actgattgcg ttggtggtgt ttaacgccac cgttgagcga
     3841 tcgacatcag acatttgcca gagcgggtta aactcaaacg tgaaatcgtc cggcagcggc
     3901 ttgccaagtt ccgaacgatg catgatgtcc agtatccgcc gcaccggaag acgtaaacgc
     3961 ctctcctgca acgaactgat gcgatcgtaa tagttggcaa ggtctgcatc accggtagaa
     4021 aatcctttcg gggactgtcc gaacaaccgc accagtggga taccaacagc gccactaatc
     4081 tgttctgcaa actgcgaaag gatgtcatcc agaccactga agctgtactg atgggtttca
     4141 aacttatccc gcgagtccat gagcgtcatg ccttcattac tctggaactg tcgaatcagg
     4201 tcgatattct tcagcaacgc ttcatacgca ggaccaccaa gtgcgataag ctcgcgtagc
     4261 ttctccacgc tgtaggtacg caaatgcgcc ttgtagacca gctgcgccgc gccgacagta
     4321 gcgctgtcga acgcggtaag acgatcccag atacgctcta caaccgacat tccccattcg
     4381 ttctcggtca tcttctgctg aaatggcaac gtgacgccat caaagcgaat caggcgactg
     4441 tgatgaatgc gccaggcagg aattcccgtt gctgtggtca ccacatcgta aaactcaggt
     4501 ttacccaggt ccggccccat atctttaatg cggcgggtca gtgccgggtc gaccatccag
     4561 cggtcgagcg ggagaatccc cttaaacttg cccttaccga tggtttcggg tcgcagcggg
     4621 gtcattggtg cctgcccctc aatcatgatg aaacccaccg cgccgccgta gaggcgcgac
     4681 catttcagca cgtcattcag cgcatcccag atttgcaact catccagttg tgattcgaga
     4741 atgccacgat cttttgcatc aatttccgaa gtgatgcgaa tgcctttgcg ggtcatatca
     4801 tccgggatag catcgactgc ttcgccgatg atccaggatg aacgatagga ccattccacc
     4861 agcatgcggt tacgactggt gaaattagcc cggtaggtgg atgctgagtg ctggttaggt
     4921 gtctgcatcc ctactcgggc aataaaattc tcataaccat cagctgtggc ctgcacagtt
     4981 cgccgcaggg cttgtttgtt tcgtgccatc aggcctgtct ccccagctgt tcccagatat
     5041 ccagcgatgt atcgattggc gcgaaggcca taatgaatgc atcagcaacg tttggtgacg
     5101 gtatctcgcg ttttgcgagg tcttttttac tttcgaccat cacacgtccg ttacggtcga
     5161 aatcacgatg aggtgttgtc agttccagtt tcagcttttc aagcaacgga caacgagaat
     5221 ctatgctgat cagctcatcc acaggatact gttctccgtt gttaatggcg ttaaacgtat
     5281 ttctgaaacg gtcagccacc atgcctgagc cttaagattt gcgaaaaagt ctttgttggg
     5341 gatgccgttg tactcgtcat ctggctcatg cacaccagca ccggcgttaa acctctggta
     5401 attcacacgt cgcgcgtatg cgttctcgct cttccggtca gcgttaattt cagagaattt
     5461 agcaccggca gacgcaccaa caccgataga gtcgtaaaca atatctgctt cacgctccag
     5521 cgccgcctga taagtacgct ggcagctctt cagtaattca tcttctttgg ccttccattc
     5581 gtcggcccag aaaacaacgg atccgtgacg gtaaacgtta gcgcacttat ctgtaccact
     5641 gtcagccacg tcaaagccaa tacgctt
//
LOCUS       DADLTY010000228         5635 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.231, whole genome shotgun sequence.
ACCESSION   DADLTY010000228 DADLTY010000000
VERSION     DADLTY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.231"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            84..299
                     /locus_tag="JN334_003864"
     CDS             84..299
                     /locus_tag="JN334_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY7439837.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            292..678
                     /locus_tag="JN334_003865"
     CDS             292..678
                     /locus_tag="JN334_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HAY7439838.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            747..1370
                     /locus_tag="JN334_003866"
     CDS             747..1370
                     /locus_tag="JN334_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY7439839.1"
                     /translation="MSDISRVKILSALMDGRAWTATELSSVANISASTASSHLSKLLD
                     CQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHGVHAKVSTPVHLRKARTCYDHL
                     AGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSER
                     RFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(1348..1971)
                     /gene="tetR(B)"
                     /locus_tag="JN334_003867"
     CDS             complement(1348..1971)
                     /gene="tetR(B)"
                     /locus_tag="JN334_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HAY7439840.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2053..3258
                     /gene="tet(B)"
                     /locus_tag="JN334_003868"
     CDS             2053..3258
                     /gene="tet(B)"
                     /locus_tag="JN334_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HAY7439841.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3371..3964)
                     /gene="tetC"
                     /locus_tag="JN334_003869"
     CDS             complement(3371..3964)
                     /gene="tetC"
                     /locus_tag="JN334_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HAY7439842.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4052..4462
                     /locus_tag="JN334_003870"
                     /pseudo
     CDS             4052..4462
                     /locus_tag="JN334_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4478..>5635)
                     /locus_tag="JN334_003871"
     CDS             complement(4478..>5635)
                     /locus_tag="JN334_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HAY7439843.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1561 a         1041 c         1224 g         1809 t
ORIGIN      
        1 gtcatcgggc attatctgaa cataaaacac tatcaataag ttggagtcat taccgaccac
       61 tattaagtca tgtagctggt tttatttcaa ttgaacgttt tcaaagtcta gctacagaag
      121 gaaaaatgtt atcgctatct tggtgggaaa acgaatacgc agttctgcaa tggaaaaatc
      181 atgttttaca tgcgaaagct caacaagaag ggcgagagtc aatatttgat ttttacaaaa
      241 ttagtattgc tcatattact cgcgaatatt catttaaaaa ggacaaggat aatgtttgat
      301 gttcacgttg ttttagataa tcaaatagga caattagcat tactaggaaa aacattaggt
      361 aataaaggta ttggattgga agggggaggg atatttacgg ttggtgatga atgccatgct
      421 cattttcttg ttgaacaagg aaaggaagct aaaatagcgc tagagcaagc tggactgtta
      481 gtacttgcga tccggacacc attaattcgt aagttaaaac aggaaaaacc gggggaactt
      541 ggcgaaatag cacgagtatt ggcggagaat aacattaata ttttagtgca atacagtgac
      601 catgctaacc aactgatatt aataacggac aatgatagta tggctgcatc tgttacgctc
      661 ccttgggcaa taaagtgaac ttgcgatggc taatttaata cgaaaagagg ttacctttga
      721 gtcctcaata gccgctaggg gcggctatgt ctgacatttc acgagttaaa atactcagtg
      781 ctttgatgga tgggcgagct tggacggcca ctgagctaag ttctgtggcg aatatatcag
      841 cttcaacggc gagcagtcat ttatctaaat tattagattg ccagctaatc acagtagtag
      901 ctcaaggcaa gcatcgttat tttcggctag caggaaaaga tattgctgaa ttgatggaaa
      961 gtatgatggg gatctcctta aaccatggcg tacatgccaa agtttccacg ccagtgcatt
     1021 tacgaaaagc acgtacttgc tatgatcatt tagctggcga agttgccgtt aagatctatg
     1081 attccctttg tcaacagcaa tggatcactg aaaatggttc aatgatcaca ttaagtggta
     1141 ttcaatattt tcatgaaatg ggaattgacg ttccttccaa acattcacgt aaaatctgtt
     1201 gtgcgtgttt agattggagt gaacgccgtt tccatttagg tgggtacgtt ggagccgcat
     1261 tattttcgct ttatgaatct aaagggtggt taactcgaca tcttggttac cgtgaagtta
     1321 ccatcacgga aaaaggttat gctgctttta agacccactt tcacatttaa gttgtttttc
     1381 taatccgcat atgatcaatt caaggccgaa taagaaggct ggctctgcac cttggtgatc
     1441 aaataattcg atagcttgtc gtaataatgg cggcatacta tcagtagtag gtgtttccct
     1501 ttcttcttta gcgacttgat gctcttgatc ttccaatacg caacctaaag taaaatgccc
     1561 cacagcgctg agtgcatata atgcattctc tagtgaaaaa ccttgttggc ataaaaaggc
     1621 taattgattt tcgagagttt catactgttt ttctgtaggc cgtgtaccta aatgtacttt
     1681 tgctccatcg cgatgactta gtaaagcaca tctaaaactt ttagcgttat tacgtaaaaa
     1741 atcttgccag ctttcccctt ctaaagggca aaagtgagta tggtgcctat ctaacatctc
     1801 aatggctaag gcgtcgagca aagcccgctt attttttaca tgccaataca atgtaggctg
     1861 ctctacacct agcttctggg cgagtttacg ggttgttaaa ccttcgattc cgacctcatt
     1921 aagcagctct aatgcgctgt taatcacttt acttttatct aatctagaca tcattaattc
     1981 ctaatttttg ttgacactct atcattgata gagttatttt accactccct atcagtgata
     2041 gagaaaagtg aaatgaatag ttcgacaaag atcgcattgg taattacgtt actcgatgcc
     2101 atggggattg gccttatcat gccagtcttg ccaacgttat tacgtgaatt tattgcttcg
     2161 gaagatatcg ctaaccactt tggcgtattg cttgcacttt atgcgttaat gcaggttatc
     2221 tttgctcctt ggcttggaaa aatgtctgac cgatttggtc ggcgcccagt gctgttgttg
     2281 tcattaatag gcgcatcgct ggattactta ttgctggctt tttcaagtgc gctttggatg
     2341 ctgtatttag gccgtttgct ttcagggatc acaggagcta ctggggctgt cgcggcatcg
     2401 gtcattgccg ataccacctc agcttctcaa cgcgtgaagt ggttcggttg gttaggggca
     2461 agttttgggc ttggtttaat agcggggcct attattggtg gttttgcagg agagatttca
     2521 ccgcatagtc ccttttttat cgctgcgttg ctaaatattg tcgctttcct tgtggttatg
     2581 ttttggttcc gtgaaaccaa aaatacacgt gataatacag ataccgaagt aggggttgag
     2641 acgcaatcga attcggtata catcacttta tttaaaacga tgcccatttt gttgattatt
     2701 tatttttcag cgcaattgat aggccaaatt cccgcaacgg tgtgggtgct atttaccgaa
     2761 aatcgttttg gatggaatag catgatggtt ggcttttcat tagcgggtct tggtctttta
     2821 cactcagtat tccaagcctt tgtggcagga agaatagcca ctaaatgggg cgaaaaaacg
     2881 gcagtactgc tcggatttat tgcagatagt agtgcatttg cctttttagc gtttatatct
     2941 gaaggttggt tagttttccc tgttttaatt ttattggctg gtggtgggat cgctttacct
     3001 gcattacagg gagtgatgtc tatccaaaca aagagtcatc agcaaggtgc tttacaggga
     3061 ttattggtga gccttaccaa tgcaaccggt gttattggcc cattactgtt tgctgttatt
     3121 tataatcatt cactaccaat ttgggatggc tggatttgga ttattggttt agcgttttac
     3181 tgtattatta tcctgctatc gatgaccttc atgttaaccc ctcaagctca ggggagtaaa
     3241 caggagacaa gtgcttagtt atttcgtcac caaatgatgt tattccgcga aatataatga
     3301 ccctcttgat aacccaagag ggcatttttt acgataaaga agatttagct tcaaataaaa
     3361 cctatctatt ttatttatct ttcaagctca ataaaaagcc gcggtaaata gcaataaatt
     3421 ggcctttttt atcggcaagc tcttttaggt ttttcgcatg tattgcgata tgcataaacc
     3481 agccattgag taagttttta agcacatcat catcataagc tttaagttgg ttctcttgga
     3541 tcaatttgct gacaatggcg tttaccttac cagtaatgta ttcaaggcta attttttcaa
     3601 gttcattcca accaatgata ggcatcactt cttggatagg gataaggttt ttattattat
     3661 caataatata atcaagataa tgttcaaata tactttctaa ggcagaccaa ccatttgtta
     3721 aatcagtttt tgttgtgatg taggcatcaa tcataattaa ttgctgctta taacaggcac
     3781 tgagtaattg ttttttattt ttaaagtgat gataaaaggc acctttggtc accaacgctt
     3841 ttcccgagat ctcatctatt gaaacagctt gatagccttt ttcaacaaac aatattcgtg
     3901 ctgagttaac cagtgattga taggtactct taaaattttc ttgttgatga tttttatttt
     3961 ccatgataga tttaaaataa cataccgtca gtatgtttat ggtatcatga tgatgtggtc
     4021 gtgacaatct taagaacatt taggttattt tatgtatatt gaacagcatt ctcgctatca
     4081 aaataaagct aataacatcc aattaagata tgatgataag cagtttcata caacggttat
     4141 caaagatgtt ctattatgga ttgaacataa tttagatcag tctttactgc ttgatgatgt
     4201 ggcgaataaa gcgggttata ccaagtggta ttttcagcgg ctgttcaaaa aagtaacagg
     4261 ggtcacactg gctagctata ttcgtgctcg tcgtttgacg aaagcggctg ttgagttgag
     4321 gttgacgaaa aaaactatcc ttgagatcgc attaaaatat caatttgatt cccaacaatc
     4381 ttttacacgt cgatttaagt acatttttaa ggttacacca agttattatc ggcgtaataa
     4441 attatgggaa ttggaggcaa tgcactgaga gatcccctca taatttcccc aaagcgtaac
     4501 catgtgtgaa taaattttga gctagtaggg ttgcagccac gagtaagtct tcccttgtta
     4561 ttgtgtagcc agaatgccgc aaaacttcca tgcctaagcg aactgttgag agtacgtttc
     4621 gatttctgac tgtgttagcc tggaagtgct tgtcccaacc ttgtttctga gcatgaacgc
     4681 ccgcaagcca acatgttagt tgaagcatca gggcgattag cagcatgata tcaaaacgct
     4741 ctgagctgct cgttcggcta tggcgtaggc ctagtccgta ggcaggactt ttcaagtctc
     4801 ggaaggtttc ttcaatctgc attcgcttcg aatagatatt aacaagttgt ttgggtgttc
     4861 gaatttcaac aggtaagtta gttgctagaa cccatggctc ctttgccgac gctgagtaga
     4921 ttttaggtga cgggtggtga caatgagtcc gtgtcgagcg ctgatttttt cggcctttag
     4981 agcgagattt atacaataga atttggcatg agattggatt gcttttagtc agcctcttat
     5041 agcctaaagt ctttgagtga ctagatgaca tatcatgtaa gttgctgata ggtttccagt
     5101 tttccgctcc taggtctgca tattgtactt ttcctcttac tcgacttaac cagtaccaac
     5161 ccagcttctc aacggattta taccatggca ctttaaagcc agcatcactg acaatgagcg
     5221 gtgtggtgtt actcggtaga atgctcgcaa ggtcggctag aaattggtca tgagctttct
     5281 ttgaacattg ctctgaaagc gggaacgctt tctcataaag agtaacagaa cgaccgtgta
     5341 gtgcgactga agctcgcaat accataagtc gtttttgctc acgaatatca gaccagtcaa
     5401 caagtacaat gggcatcgta ttgcccgaac agataaagct agcatgccaa cggtatacag
     5461 cgagtcgctc tttgtggagg tgacgattac ctaacaatcg gtcgattcgt ttgatgttat
     5521 gttttgttct cgctttggtt ggcaggttac ggccaagttc ggtaagagtg agagttttac
     5581 agtcaagtaa tgcgtggcaa gccaacgtta agctgttgag tcgttttaag tgtaa
//
LOCUS       DADLTY010000229         5575 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.232, whole genome shotgun sequence.
ACCESSION   DADLTY010000229 DADLTY010000000
VERSION     DADLTY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.232"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..386
                     /locus_tag="JN334_003872"
     CDS             <1..386
                     /locus_tag="JN334_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7439844.1"
                     /translation="LLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYSDDGL
                     AEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGIDPEAYLRHI
                     LSVLPEWPSNRVDELLPWNVVLTDK"
     gene            418..1032
                     /locus_tag="JN334_003873"
                     /pseudo
     CDS             418..1032
                     /locus_tag="JN334_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1201..5295)
                     /gene="sepA"
                     /locus_tag="JN334_003874"
     CDS             complement(1201..5295)
                     /gene="sepA"
                     /locus_tag="JN334_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HAY7439845.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1370 a         1395 c         1323 g         1487 t
ORIGIN      
        1 cgttactgac gtcgctgaat gaatggatgg tggagaagaa tggtacgctg tcgaaaaaat
       61 ccagactggg cgaagcgttc agctatgtac tgaatcagtg ggatgccctc tgttattaca
      121 gtgatgacgg tctggcggag gcggacaata acacagcaga aagagcgctt cgtaccgtct
      181 gtctcgggaa aaagaattac atgttcttcg gcagcgatca tggaggcgat cgcggagctc
      241 tgctgtacgg actaatcggg agctgtcggt tgaacggtat cgatccagaa gcgtatctgc
      301 gccatatcct gagcgtactg ccggaatggc cttccaaccg agttgacgaa ctgctgccat
      361 ggaacgtagt tctcaccgat aaataagcgt caatacggcc ttccgttgac gcttacagaa
      421 ctcctgtggc gcgagagtga aaaactgcgt cgttataaaa aggaggcccg gttgccagtt
      481 gccaaaacgc taagcgaata cgacttcatt cagttgccgg aactgaatgg agctcagttc
      541 cagcaactct gtgaaacgac agactgggtt gatgctggag aaaacgttct gctgttcggg
      601 gccagcgggt tggggaaaag ccatctggcg gcagcgatcg tggatggcgt agtaggccag
      661 ggctaccggg cccggttcta cagcgcagga gagctgttgc aggaactacg taaagccaga
      721 gctcagttga aactgaatga gctgctactg aaactggatc gctaccgggt gatagtggtg
      781 gatgatcttg gctatgtcaa acgcgacagc gccgaaacgg gtgtgctgtt cgagttaata
      841 gcgcatcgct atgaacgtgg gagcctggtg ataaccagta accatccgtt cagcatgtgg
      901 ggcagcatct tcgtggatga gactatggcg gtggcggcag cagaccgact gatccatcac
      961 ggatatatgt tcgaactgaa aggggaaagc tacaggaaaa agacagcgaa ggcagtaaca
     1021 agcgtgactt gatgtcgcac tgaaggggcg gccagtatag ttggcgccag tcggcaaaac
     1081 tagttgacgt ctaatacctg gacggtcact tctgagaaaa ggcatttaca cagaatctta
     1141 aacaggctca cggagttctt tcagaaaaag gcctgccgaa tggcagacct atcccattcg
     1201 tcagaaaaca taacggaagt tggcgtttat cgcattatcc acattatatt tcccgaacgc
     1261 tgatttctcc agctccagtc ccaaacgcat attatcctta atttccgcat tcatccctac
     1321 cgtcatcagc atcctgctat ctttttcacc ttcgaaacgt ttctctccgg aagcatcctg
     1381 cagaaccgtt tctccgttcg ccagcaggtc gaactgataa cccagcccgg ctcgagctgt
     1441 gattttccag tcgtctccgg agaaggctct tcccacgtca acaccagtac ggccaatcag
     1501 tgggttataa tccttgtctt tcatgctcag agccattccc cggtcttccc agctaaaagc
     1561 ttttcctgat acagaaccgt aaaccagctc tatctgtggc tccacccagg actctttcgt
     1621 caggtggtaa cgataaccaa cttcagcacc ggcataccag gaatgagagc tgtagtcttt
     1681 tgttcccagt gaggcaaaat tcgccgtgtg ctgattatca tgatggagat atttaccaat
     1741 caggtcaaaa taagctccgg aattaaagag tgcagaggca tacagaccgc cacccacgga
     1801 tttgtttttt ccactgaatg cgtggctgct tgcattgctg tccgtatagg tcagcaatgc
     1861 ccccgtaaat aaatccacac cgtccagctc atgctttctg tcgacaccaa tctgaacgtg
     1921 agtgtagtta tcgctgtagt caccatctgc cgaaccggta ccattcatta tgcgtgccca
     1981 gacaccggca tccccctgag tatcccgcag gtcacccata cgtttgttca ggttattgac
     2041 ctctgtaagg aaggacttat aacctgatgc cataaagtct tttgcggctt tcgaggctcc
     2101 ggcatcagcg gtagtctgat atcctgtcag cacccatttt gtggcatcat ccgttttttc
     2161 cgtgctgatt actggcgtaa cgttactgaa accaatctgc tgcgtaccgg cagtgaacgt
     2221 tttctcatca ctccctttcg gtgcggtaat taacgtcacg ctcatttcac tggctggttt
     2281 gtttaaaaag tcaaccagga cgctattatt actgcctgac agtttgtctg tgacattcaa
     2341 ctggtcagcc cggctgttgt tggtatgcat caggaacgtg gtattatctg cagaaagctc
     2401 ttttacttgt aaagtggcaa agtgtttccc gtcccccagt tcaattcgcg agcccttagc
     2461 cttcagtgca ccggcctctg attttccgtc agagtaccac agagcattga tcatatttac
     2521 cgtactctgt gcccccgtaa tactgcctct cagaacagcg ttatatccct tcattaagga
     2581 gctgaataac ggagagtccg tcttccctgc atcagcatca ctgtctccca gattaatggt
     2641 tgccgcagtg gttcctgcat gaatatccga actcagatag cccatatttt taacagaaaa
     2701 gctggcgtta tcttcgaggt taatcccttc tgttgtagaa attacgggtg agtaataccc
     2761 ctgtttctgc gcagaaggca tccccgtcag ggtcagagaa gcattttctt ttacatccac
     2821 agccccggtg ctgaaaatac cgctctgagc cgtaagtttt gctccttttc cgagggtcag
     2881 gctgctgttg acaaagctgc caacacggtc aaaaacagca ttctgagagg tcacactcac
     2941 ggtgctatca taagcctcaa taccaccggt gaatctctcc atgatttgca gggatgattt
     3001 attttccagc gtaacatttc cgctgtaatc actctggtca ccaactgtgg ttgcagtggc
     3061 actcccttca acagaggaga ccgcattacc ggttccgtca ctcctgtcag taaagacacg
     3121 gctacttccc agaatgactg tacttccatt ggtggcgtta atatctccct gcatcttgcc
     3181 gtttctggac agatggaatg ttgccgcatc gagtttcagt ttttccagcg taaactgacg
     3241 ggtttcccag tcgctctggc tggaagaagt cgtcgttcca gcatgaataa ctggatgacc
     3301 ctggaatatc agcgtgccgg acttcttggt tatcgaacct tctggcaggt taactgaacc
     3361 atcaagagcc agtacatcct tacctgagag ctgcggaata ttcacatcca tattgccgtt
     3421 tagttggccg tgatacatat aaggctggct gctggcctca attttttgtt gcttcacagt
     3481 atcaatggct ttattacgat cttttccgac attctgccag acactgctgt tgttcagatc
     3541 ggagaagaac ggggaatatg aactcgcttt caggatgaaa tattgcttgg tagaactgtc
     3601 ataatatagg tctcccggcg ctcctctccc cccaaaaata ctgacagtat taaccggtac
     3661 attgatgtca ctggctttta atgtctgaag gtcgagtgtt accgttgatt ttttctcact
     3721 gttattggta atgatggccc cgtagtcagc cgcctgaaga cgggtaaagg tcagattatt
     3781 accattcagt tccagacgtc ctccacggta tccccacgaa atattatccg gattaacttg
     3841 ctgtgagtca gaaagcacaa ctgtcggacg accgctggca ataccaacag agctgaaagc
     3901 ctgtaccttt ccgtcagcat ccgcctgctg gttgagaata acggtaccgt cccccacctt
     3961 gagtccgcct gcattcacac cggtcccgtt tactgtcagg gtaccttcgc cagttttatg
     4021 caggttatca ccggcaactc cgttcacctg ccacagaacg tgggttcctt tatcggtgat
     4081 aatcccgccc cccatccatg tctggccgtt cagggcggac accctgtaat cgccggcaaa
     4141 ctgcagatac ccagctccct gattaacgga attctgcaga acgacctcgc cattattgcc
     4201 ggtgaacagg aggttttttc ctgcgttcag atcatttcct ttctttccat gcatatccca
     4261 agtcgtgttt ccctgagtca gtgtgccggt gccattagcc gcatcatatt tccactgcag
     4321 tactccttca ccagaggtgt aggctatagt tttgtcaaaa tcattttgcg gttgctgacc
     4381 aaggaaatcc tgagtggtga cgacccagtt atttccatag ttagttcctg aggaaagcac
     4441 gccaatcagt acccattttt tttgcaggga atcataagca aacagggggg agccgctgtc
     4501 ccccatattc agataattag ccagaggtcc attcgcggga ttaaaaatgt caccggtctg
     4561 agctgtaatc atctgtccgt tgttatagga gttcagcgcg ctgacggttc ccccaacaag
     4621 aaatccgcca ttggtataca cttgggtgcg attaccattt ttgtctttga cgtactgcat
     4681 ccccccccca aggcgataga attcagtaaa gcgtccgccg gcctgatagg caccgctcac
     4741 agccccgatg tcagacactt ctgccggcgc cacctctgtc accagtttac tcaggcggcg
     4801 ggttttaata tcaagaccgc tattattatt tgtaccaacc gcagtatagg tgtttttggc
     4861 gtatccgaag ctcatcgtgt cagaaccgct gacatgtttg gcggtaacca catactgagg
     4921 ggagtaaagt gtatggtctc ccggaggcag gctaccggta gtaatagtgg cactactgaa
     4981 atcagccatc ggggctttat caagtttacc gaccagattg ccctgcttgt cgtaaatgga
     5041 aatattcgtg gtccccggcg taaactgacc tttattctcg gcaaagtcgc ggaatatctg
     5101 ataagggatc tctgcactga ccgttgctga cagagctggc catgccgagg aaagtgataa
     5161 agctgcaaca gacgtaagaa taacccgacg tgaaagtctg cgatggcttt tgcaggtaac
     5221 cctgcgggcc agttcggaga cagcaatcag gcttttggtt atatggcaat acttaagata
     5281 atatattttg ttcatataga aacatcaacc taattttgac aaagattaat tacataggat
     5341 gtagatggag cagtccgaga ttctactaat atcaattatt aagcatctgt tttttagttg
     5401 gtgtaatata atttttatta aggtaagcgg aaaaacaacc aaaaaaaacg caagatgtta
     5461 tttttttatc attttgagcc agtttggctg gggggatact cttctcttaa gcttcttgct
     5521 caactttaaa aatagttatg ccagttcggt atcgataagg ggcagtttgg atatc
//
LOCUS       DADLTY010000230         5571 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.233, whole genome shotgun sequence.
ACCESSION   DADLTY010000230 DADLTY010000000
VERSION     DADLTY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.233"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(57..475)
                     /locus_tag="JN334_003875"
                     /pseudo
     CDS             complement(57..475)
                     /locus_tag="JN334_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            525..1703
                     /gene="ltrA"
                     /locus_tag="JN334_003876"
     CDS             525..1703
                     /gene="ltrA"
                     /locus_tag="JN334_003876"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY7439846.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1786..1965
                     /locus_tag="JN334_003877"
                     /pseudo
     CDS             1786..1965
                     /locus_tag="JN334_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1995..2477)
                     /locus_tag="JN334_003878"
                     /pseudo
     CDS             complement(1995..2477)
                     /locus_tag="JN334_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2733..3713)
                     /locus_tag="JN334_003879"
     CDS             complement(2733..3713)
                     /locus_tag="JN334_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAY7439847.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3713..4912)
                     /locus_tag="JN334_003880"
     CDS             complement(3713..4912)
                     /locus_tag="JN334_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAY7439848.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5234..>5571)
                     /locus_tag="JN334_003881"
     CDS             complement(5234..>5571)
                     /locus_tag="JN334_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY7439849.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1539 a         1270 c         1316 g         1446 t
ORIGIN      
        1 gcagcataag ttatccatgc tctgaggtgg gtagaggtgg gccctccggt cagggtttac
       61 aggagcacag accaaacgac ccggtgctgc ttcattctaa ggaatgaggt ggtgagcgac
      121 tcgggaaaag gcagagatct actgtcacgt gggttcggga agctgaacga aagtaaactg
      181 tccgatgaag catcgaaatc ttagatgatg ccaaaatggg gccgtacagc cgtcccctga
      241 taagcgcaga agacacctgc ttactgactg cgcggcatcc ggtgtgaagg cagcaggatc
      301 ccgggccaga ccatctcagg gaacgtgaga acctgtttcc ggcacgtatc gggaaacagg
      361 ggcggaccca ttcgtagtag tgatgaaaga tctgtaatgg gtctggagcg aagggatggg
      421 gatcacattc attgcagaag acacaaccca aaaggggatg acgtaactga tgcaacgaaa
      481 atcatttgaa ataccaaaag cactggtctg ggcatcatat ctggatgtgc gtcgcaacaa
      541 gggagccccc cggatgcgac gggcagactt tgaaaatgtt cgaccagcaa cgtgatggca
      601 atctatacaa gatctggaac cggctatgtt cgggaacatg gtttccgcca ccagtgctgg
      661 aaaaacggat cccgaagtcg aatggcaagg aacgcattct ggggatccca acagtatccg
      721 atcgaattgc tcagggagcg ataaaacttt tcatggaaga aaagcttgac ccgattttcc
      781 atgctgattc atatggttac cgaccaggca aatcagctca tgacgctctg aaacaatgtg
      841 ccatccggtg ctggcgttat agctggatac tggaagtgga catcagtgcg tttttcgacc
      901 atgtgagaca tgatctggtg ctcaaagctc tggaacacca cgggatgcct aaatgggtca
      961 tcctgtactg tcgacgctgg atggaagcgc caatgcagag ttgtgaaaat ggagaattaa
     1021 taacccgaac acgaggcact ccgcagggcg gggtcattag tccgctactt gctaacttgt
     1081 tccttcacta tgcatttgat ttgtggatgg aaagagaata tcggggggta ccgtttgaga
     1141 ggtacgctga cgatattgta gtacattgtt cacgaatgag tgatgcaaca aggctaaaga
     1201 acaggttatc ggagcgcttc tcggaagtcg ggctggtcct gaatgcaggg aaaacgaata
     1261 tcgcctacat tgacacgttt aaaaggagaa acgtcgcaac gagtttcacc tttctcggat
     1321 atgacttcaa agtgcgtacg ctgaagaatt tcaacggcga acggtaccga aaatgcatgc
     1381 cgggtgcgtc aaatgcagca atgcgcaaaa taacagaaac aatcaagaag tggcgtatac
     1441 atcgctcaac agctgagagt ttgctggatt ttgcgaggcg ctacaatgcg atagtgagag
     1501 gctggatcga gtactacgga aagttctggt ccagaaattt caactatcga ctgtggagtg
     1561 caatgcagtc acgtctgctc aagtggatgc agtctaaata cagactttcg aaccggaagg
     1621 ctcagcgaaa gctgacgctg gtaaggaagg agtatccgaa gctatttgtt cactggtatt
     1681 tactacgtgc atcaaatgag tgatcaagag ccgtatgacg cgagagtgtc acgtacggtt
     1741 ccgtgagcag ccggggagtg aaattctctc cggctgactc gactcgcggg ttccgacagc
     1801 gatgacgaac atgcggcggt gttgtactcg ctgatcggca catgccgtct gaacaatgtg
     1861 gaaccagaaa aatggctgcg ttacgtcatt gagcatatcc aggactggcc ggcaaaccgg
     1921 gtacgtgatc tgttgccctg gaaagttgat ctgagctctc agtaaatatc aatacggttc
     1981 tgacgagccg cttacttttg gcgtaattct gctgcgcctt tactgaacac aatgccgtat
     2041 tcagcaagaa gtccacgaat gtgatttatc agttgtgtcc tttccattga aaccaaccga
     2101 cgcatattca aaaaagtctg gacgtcctgt tgctccagag tttttattgg tacgggtcgt
     2161 atagttccat gttggcaggc ctcagctatt gcctgtgcgt cgttataatc atttttctgg
     2221 caccgctgat aagctttaac atgttgtgcc ggaagcaata ttacatcatg gtcgagtttc
     2281 ctgatttccc gggcccagta atgagatgcc ccgccagcct ccatcgcaac agtcatttta
     2341 ggctgttgca ccagccagtt aagcagttga ttgcgagaaa gtttggtctt tcctgcgggt
     2401 ttgccatgag cattgatact gaaaagataa aagttcgttt tagccagatc aatgccggtg
     2461 attttctgtt cagacatggt atcccgctta cttttgttgt taaagtttat ccatcatggc
     2521 gcaattgaag gctgccgtga aagaggggga agactatctc atcggttact gttaaacggc
     2581 taagagaaac tatctttttc aagcaaagaa gatacggtga aacaggcgat ttcaccgtga
     2641 aatactgttc aaaaaaaacg ctaataaaag caaaatttct tttaaaacag ccaattaaaa
     2701 actggcgagt tgctggcgaa aaacttgaat tttcacagcg aattcagatt atcttttagt
     2761 acaagagcaa tcatacgatc taacttatcc tgaacttcca acggtaatct gccgaattca
     2821 tatgagaacg tacgtccctt tactcgtttt cgagcaaatt tatctttact atcaaatttc
     2881 cataacaaag ttacaaccgc tttatccttt actcctgaat caagcaatga agacatttcc
     2941 ttggttataa gccgcataat actgttttta acctcgtctt cagaaagatt gtcatcagca
     3001 gtaataagct caatactcgg cgagatattc ttcacaactt catcaataga tatatcaccc
     3061 agcctcagtc gttcacttaa ttcagccagt tgcctgtaat cggcgtatgt cagctcagac
     3121 tgaacaggaa aaagagacac aaaatcttta ggtacggacg ctgcttggag ggcacgagtc
     3181 accttagctg ccgacaatcc ctctctttcc gcaatttgct tctggttcat accggcctct
     3241 ttgagacgta ctaaacgcaa cccaatttca cgaatattat gttcaagtga cgtctgcaaa
     3301 tctttcgcca gatgctgagc ttcagaaaca gtaagctcat catccgttac aagtacacgt
     3361 aatccaacct tgcatagcaa agctgctgcc cgacgacggg aaccatccaa aatctcaata
     3421 agatcgccag tccgaatccc aatacatgga tagaattgtt gtaaacgtat agttctggtg
     3481 atgtctttta gtgagtccgg cgtcagggct gtctgttcgc ggccattcac ctcttcaacc
     3541 acaaaagttt gagtatccac tttgtcatga tcaaccttga tctcagtaaa ttttgccttt
     3601 cggccagtat tgagggtaaa tacatgaact ggagccgata tttcttcagt attattcagg
     3661 atatttgtat tcagcgtacg gccaatagtc ggtcggtgct ttctgttttc catcagtagt
     3721 tggcccttat aaattcaata cgatcaaaaa ctgctttagc gaaatcttct gctgccatac
     3781 gtgcattttt caaagcttca ccactgccaa cataagtaac aggattcgca gatatcacgg
     3841 tatcaaacga ctcgccgctt ctctcaaaac catcaagacg tggcattgaa acatcaagca
     3901 tgtcaccacc gaaaatctct tttgtcaggc tatggcagta tttatgatct gacttattcg
     3961 ccagtttcga catgaagcca atatttgcct gcaaacggca tgaacatcca tcctgttcaa
     4021 taatctgtac aagttcaggt aaacgagcca aatatttaag cgtggaatgg aaatcaactt
     4081 gtgctggcgg caccggtgta aacatgatat ctgcggcagc aatggcattt ttcaaaaatg
     4141 cgtccagatg cgggcctgta tcaataagga tgaagtcgaa atcatgcttc agcttatcaa
     4201 taatattctc acgcaatatc gcgtgtttgt tttgtcctaa tagatgctcc tcgcacagag
     4261 tatcccagtt tgatgcaata aaggcgtcgt caatagaagc cggcataaca taaacgccag
     4321 ggataactga cggaacgata aaatcttcca gtaattcctc acgactcacg ttctgcaaca
     4381 tagcctgcgg tgcggttgta tcaaccagcc ccaccgcatg caagtagttc agaaacatag
     4441 tggctgatga ctggggatct aaatcaagga gcagaatacg taaatcttct gacagcaggt
     4501 gaggatgagc acgcagggcg tgagctacgg aaacactact cacggtttta gacacaccgc
     4561 cttttagact accgatgaat atggaatatg cctcactata acgatcgcga tatttaggaa
     4621 tgccacgatg tgcatagata tcgataatgt tctggatggt taatgcatat tttgtcgcgg
     4681 ttcctgctgg gcgtttttca aactgatagc cctgagcctc catctcaccg actgcaccat
     4741 ccacgcttgc gcgcgttaat tttggaagct tagctacagc agactttgaa tagacttgat
     4801 aaaaaacatc agcctgaaac tcctgctttt gctcctgaat ctgttttgtt agggcgagca
     4861 acattttatc tgcacgctgc gcaaccttac ttaactgctc aaaactagtc attccccctc
     4921 cgtgctatgt aggattttaa cttattacaa aaaaaactgc attagcaggg taatgcatgc
     4981 aagtacatat tgtacatgct tatattaaaa atgcgcgcat tgctgattta ttaatcatcg
     5041 gaatgaatat tactgtaaga tagtatgcct ttccgggaga aagacgctga tttcccccga
     5101 ataatccagt tcaccaccac actccggaca acagctttct tcaggctcca gatggtgtat
     5161 ttcgcgggaa acatgttccg gtaacggacg gcgatgacgt gactgtcgca acaggcgggg
     5221 gtagtgcttc caatcagtag aacatgtgct tttcaatgaa ggagccgatg actttttcgt
     5281 ggatttccac ggaacgcgag aagcagattg ttttacgtgc cagccgcttg atgcgggtgc
     5341 ggagggtccg gttgttacgt gcaatacgtt gcgtgaagat tttgccggtc aggtgtttct
     5401 ccttcggcac ttctctggcg tagctgcccc agtcatcgct ggtcaccatg ccgatacaga
     5461 acggagtcaa cagggctaac agttcccgac aggtctcatc attccgtggc ccgaaagtgt
     5521 aagccaacac accgccagtt ttagtgttgt acgcatacca gagccagtgt t
//
LOCUS       DADLTY010000231         5557 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.234, whole genome shotgun sequence.
ACCESSION   DADLTY010000231 DADLTY010000000
VERSION     DADLTY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.234"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..641
                     /locus_tag="JN334_003882"
     CDS             <1..641
                     /locus_tag="JN334_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439850.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="JN334_003883"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="JN334_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAY7439851.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="JN334_003884"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="JN334_003884"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HAY7439852.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1376 a         1360 c         1539 g         1282 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatcg
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagctatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaa
//
LOCUS       DADLTY010000232         5485 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.235, whole genome shotgun sequence.
ACCESSION   DADLTY010000232 DADLTY010000000
VERSION     DADLTY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.235"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..1630)
                     /locus_tag="JN334_003885"
     CDS             complement(<1..1630)
                     /locus_tag="JN334_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAY7439853.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2530)
                     /locus_tag="JN334_003886"
     CDS             complement(1646..2530)
                     /locus_tag="JN334_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY7439854.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2541..3146)
                     /locus_tag="JN334_003887"
     CDS             complement(2541..3146)
                     /locus_tag="JN334_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY7439855.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5485)
                     /locus_tag="JN334_003888"
     CDS             complement(3317..>5485)
                     /locus_tag="JN334_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707671.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HAY7439856.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKESINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRRAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1580 a         1364 c         1141 g         1400 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccca gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgccc tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactctcctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc
     5401 cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt
     5461 tgatagcttg ttcaacttgt tgttt
//
LOCUS       DADLTY010000233         5398 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.236, whole genome shotgun sequence.
ACCESSION   DADLTY010000233 DADLTY010000000
VERSION     DADLTY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.236"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..497
                     /locus_tag="JN334_003889"
     CDS             <1..497
                     /locus_tag="JN334_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HAY7439857.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="JN334_003890"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="JN334_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="JN334_003891"
     CDS             2500..3114
                     /locus_tag="JN334_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HAY7439858.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="JN334_003892"
     CDS             3114..3443
                     /locus_tag="JN334_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HAY7439859.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="JN334_003893"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="JN334_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HAY7439860.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="JN334_003894"
     CDS             4529..4840
                     /locus_tag="JN334_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY7439861.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4975..5373
                     /locus_tag="JN334_003895"
     CDS             4975..5373
                     /locus_tag="JN334_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7439862.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1177 a         1344 c         1425 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggca
//
LOCUS       DADLTY010000234         5385 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.237, whole genome shotgun sequence.
ACCESSION   DADLTY010000234 DADLTY010000000
VERSION     DADLTY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.237"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            357..578
                     /locus_tag="JN334_003896"
     CDS             357..578
                     /locus_tag="JN334_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1795 domain-containing protein"
                     /protein_id="HAY7439863.1"
                     /translation="MLSQRGGLEIQSQFLRGNEQVYQCQVAFVLPGERVMMAFTYART
                     TPLTPADMTRWAEIKKNLRFRMRQEVRTN"
     gene            597..3403
                     /locus_tag="JN334_003897"
                     /pseudo
     CDS             597..3403
                     /locus_tag="JN334_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706529.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            3348..3983
                     /locus_tag="JN334_003898"
     CDS             3348..3983
                     /locus_tag="JN334_003898"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAY7439864.1"
                     /translation="MTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRET
                     GLHYNLHRYYDPDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKAN
                     RLLDSSETKVTVRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGS
                     YHWDDIKDPSVTKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            3986..4366
                     /locus_tag="JN334_003899"
     CDS             3986..4366
                     /locus_tag="JN334_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439865.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(4421..4708)
                     /locus_tag="JN334_003900"
     CDS             complement(4421..4708)
                     /locus_tag="JN334_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HAY7439866.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            4837..5346
                     /locus_tag="JN334_003901"
     CDS             4837..5346
                     /locus_tag="JN334_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HAY7439867.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT         1349 a         1251 c         1537 g         1248 t
ORIGIN      
        1 gttgttaata aaaagcttat agatttatag ttgataatta aaacgcatcc gatatttcag
       61 ctttttctat gcaagataca attttataag gtgtccattt ttatatggag gcggtagcgt
      121 gtctgttact agtggtttta tttgcatagt gaatatattg ggattgttaa atacataact
      181 atgtatccgc tctggtcgtt gtaaatattg ctaattgctt aatattatta gtgattatga
      241 ctggaaagat aaagtgtgct gtaaagcggt gatgggtatt acattcatga ttaaaaaaac
      301 agaacctgtc aataaataat tcctttatta aaacggacta tttacattat gacggaatat
      361 tatctcaacg aggcggtttg gaaatacaga gccagtttct ccggggtaac gagcaggtgt
      421 accagtgtca ggtggcattt gtattgccgg gtgagcgggt catgatggcc ttcacctacg
      481 ccagaaccac accgctgacc ccggcagata tgacgcgctg ggctgaaatt aaaaagaatc
      541 tgcgcttcag gatgcggcag gaagtcagga cgaattaatc atacagggaa cgggacatgg
      601 aagaggcatt ctgggcggca agacagggtg atgccctgct gcatacgtcg tttatggcag
      661 atgtgctggg ggcggtgctg gaggtggccg caaatgtcgt tattgatgcc ctcattgtcg
      721 gcacaacctc gttactggcg acgtcagtgg ggatcacgat ggggtgcacg gccgtcgtgc
      781 tgaccggttt tgtggcgggt gtggcgatgg tgtacaccgg cgtgacagaa aaggtcagcg
      841 aagcctgttc agccctcgca aatatgcttt tcccgcctca gattgaaggt tacatcctca
      901 ccggctctgg tgacacatgg atcaacagca aacctgccgc gcgggcagcg gcgactgcgg
      961 catcgcggca ggacatagag gcgcaggaag cgcaggcgaa ggcagaacag gaagaagcgc
     1021 agcgtcagga ggatgccaga acgttccggg atgtggccgg ggaatatctg gaaatggcgg
     1081 gggccatagc cctggcggtt aacccggtga cagggcctgt gctgatgtcc cgggctctgc
     1141 acgaccagct cagtacggaa gaagggcgtt ctgaaatggt ggatggcgtg acacattttg
     1201 tgtcggaact atggcagccc actgtggcca gtgcggcccc gggatccacc ccgacccctg
     1261 acgataaaat cgactgccat aaacatcctt cctcactgac gcagtttctc gcacaaaaaa
     1321 aagccgcctt tctggatgac cccctgggaa cggcccttaa cctgctgaat ccgggcggta
     1381 tgctggaaac cgcctttcag ggagtgaatg cggttattgg cagcatcagt aatctgttca
     1441 aaggcgatga cgagccgccc gcagcggaat atatcgcgga aggcgcgcgg gatgtgcgta
     1501 tcaacagcca gccggcgaca cgcagcgggg cacgctgtac ctgcgaggcc agggtggtga
     1561 atgagccggg gaacggggcg tttgtgtcac cggatgtgcg cattggcggt ccgttactgg
     1621 tggtcaggga tatcagaagc ggcaggtcgc agataaccct ggtggcaacc gtggcgctga
     1681 tgtttcttcg cccgggcaaa atgctttcta aaatcgcctg ttttgctgcc agtgtcggga
     1741 tgagcatgct gacgcagaag gccaccagtg cccttccgca tccggtcaat gccgccaccg
     1801 aggccaaata cctggccgat gacgatgact tcgatttcag tctgccgggc cactttccgc
     1861 tggactggca gcgcgtgtac agcagtcgtg acgaacgtac ggaaggaatg tttggtcagg
     1921 gctggagtgt aatgtatgaa gtcagcctcg tgtgcacacc gggtagcgca gacgaaaact
     1981 gcatgacctt tgtgtcgggc atggggcgac gcttggatat ggaagccgta ctgccggggg
     2041 gcgggtttta cagccccggt gaagggctgg cggtaaggcg tggtgagcag gggcactggc
     2101 ttatcagcag cgatgacggg cagtgttttc tgtttgaggc tgacccgcac cacccgcagc
     2161 gccagcggct gaaaatgctg ggcgaccgta acagcaactg tctgaatctg tactacgacg
     2221 accggggccg cattgtggaa atcagcggcg aacagcagcg tccgtgcatc cgtctgtatt
     2281 acgaactggc ggcgcatccc cggcgggtga cacagattta tcagcatttc cctgagactg
     2341 cacctttact gctgcgtcgt tacagctatg acgaggccgg ccacctgaac ggcgtgtacg
     2401 acagcacggg gcacctgctg cgtgagtttg cttacgacga aaaccactgc atgacgctgc
     2461 accgccagcc gggcggcgaa gggtattatt accagtgggg ctggtatgaa ggtccggatg
     2521 acgcggcatg gcgggtgacg gggcaccaca cggacagcgg tgaacagtac cggctggcgt
     2581 gggacctggc gcagcgcagg ctgtgcgtga cggacggact ggggcgcaca cgttatcacc
     2641 agtgggatgc gcagaatcag gtgacggcgt accaggacga agccggacag gtgacgacat
     2701 tccgctggag cgacgaagaa cgactgttgc tggggatgac cgaccctcag ggcggcaaat
     2761 ggcgctatgt gtatgacctg caggggcata tcacggagac gcacgacccg ctggggcggg
     2821 tggagcaggc gcagtggcac ccggtgtggc accagccgga aacggaggtg gacgcggcgg
     2881 gaaacctgct ggggaggcgc gcgggggaaa gggccacggc agaaaacagc gtggtgccgt
     2941 tcaaccggct gatgtcttac cgtggtgtgc actaccgtta tgacgaattt ggccgtgcgg
     3001 tggaaaagga gggccggagc ggaacgcaga gttaccgtta tgatgcggaa caccggatgg
     3061 tggaagtgac gacagcgcgg gggacatacc ggtatgtgta cgatgcactg gggaggcgga
     3121 cggaaaaaca gcacatcagc cctgacggta agccgtacaa ccggacaaaa tttttatggg
     3181 acggaatgcg gctggcgcag gagagcaggc cggagggaac aggcagcctg tacatctaca
     3241 gggaccaggg gagttatgaa ccgctggcgc gggtggataa ggcgggcaaa gagggcccga
     3301 accggatact gtattttcac acggatgtga acggcgcgcc ggaagagatg acggacagcg
     3361 acgggaaaat cgtgtgggag acggggtatc aggtctgggg taacacgata caggagaagg
     3421 accacggcgg ggtggagcag aacctgcgct accagggaca gtacctcgac agggaaacgg
     3481 ggctgcacta caatctgcac aggtactatg atccggatgt cgggcggttc atggtaactg
     3541 acccgatagg gttgagaggc ggtttaaatc tctacagtta tgcacctaat cctctaaaat
     3601 atgctgatcc acttggatta actccatgtg ctgttagtaa ccaaaaagcg aatcgtttat
     3661 tagatagtag tgaaaccaaa gttactgtta ggtctagaag tgacgctgaa caattgttta
     3721 tggatagata tttaggtcat aattataaaa atatgacagg tgagtcaggc cccagcacta
     3781 agaacttaat ggaatatctt actgaaaata aaacaaaagc aggttcatat cattgggatg
     3841 atataaaaga tccttctgta acaaaacctt catatagagt gtcaggtcat ggaccaggaa
     3901 atcctgatgg tgatttacca catttacaag ttcatcaaca tggtggaagt gttagacata
     3961 tatttttccc gtgggagaca taataatgaa caatctcaat acattattaa gtggaaaggt
     4021 tttgctcaaa gaaattagtc cattaatgcc tggatataga acatggattg aaatttcatt
     4081 gatcgatgaa ttaaaaccat cttatccttt tagattagat gaatatgcta tgatagggca
     4141 ttcaccttat gccaatgaat gtaataaaga tgaagctaaa tttaaactac gaatatcatc
     4201 atttttagct tctgacatag ataatgaata cgatccatca tatgattatg ttggaaaata
     4261 tgaaattatc gcttcactta atgagttaaa aactaggtta agtactcttc atgtaaactt
     4321 ggagcaattt ataaattcat cggaagatga tgagtatcca ttataataag acagaataaa
     4381 aagctaggaa ggttttaaat cttcccagct tttttaatat ttaactttcg ttcagcacac
     4441 taaacatccc atcccgcagt tcctgcacct cgtcactgtc agcgatgatg gtcaggcccc
     4501 cctccgaaat cttcaccgtg acaccccgtc cggtatcaaa tccggcctct ttcagccagt
     4561 cgcccttaag gtgcagactg ggatgttgtg aataataccg ggctataccg gttttgcggt
     4621 cctggtggcg ggctctgaca tagccgactt tatagcggcg ctgtgttttg gaaatagtga
     4681 cttctggcgt aatatcgtgt tcagccatga ttaactacct tgattagttg cttgcgggga
     4741 gcgcgctttc gtaatcttgc cctgctagcg gattaacttc acaaacatca tttaccaacg
     4801 cctacactta ctcctgtata ccgttcagga gcagtaatga atctacaacg atttgatgac
     4861 agcaccctaa tccgtatctt cgcccttcat gagttacatc gactgaaaga acatggctta
     4921 acgcgcgggg cgcttctcga ttatcacagc cgctataaac tcgtctttct ggcccattct
     4981 cagcccgagt accgcaaact tggcccgttt gtggctgata ttcatcagtg gcaaaatctg
     5041 gatgattttt acaaccagta ccgccaacgc gtaattgttt tgctttctca tcccgccaac
     5101 ccgcgcgatc acaccaacgt tttgatgcac gttcagggtt attttcgccc gcatattgat
     5161 tccacagaac gccagcagct ggctgcgctt atcgacagtt atcgccgtgg cgagcaacca
     5221 cttcttgcgc cgctgatgcg tatcaaacac tatatggcgc tttatcctga cgcctggctt
     5281 tcagggcagc gttatttcga actttggccg cgtgtgatta acttgcgcca ttcaggagtt
     5341 ttatgactac ccatctggcc tggtttcgcc aggatttacg tctgc
//
LOCUS       DADLTY010000235         5339 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.238, whole genome shotgun sequence.
ACCESSION   DADLTY010000235 DADLTY010000000
VERSION     DADLTY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.238"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="JN334_003902"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="JN334_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAY7439868.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="JN334_003903"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="JN334_003903"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAY7439869.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="JN334_003904"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="JN334_003904"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HAY7439870.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="JN334_003905"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="JN334_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAY7439871.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1457 g         1282 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcggg
//
LOCUS       DADLTY010000236         5218 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.239, whole genome shotgun sequence.
ACCESSION   DADLTY010000236 DADLTY010000000
VERSION     DADLTY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.239"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(93..2096)
                     /locus_tag="JN334_003906"
     CDS             complement(93..2096)
                     /locus_tag="JN334_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HAY7439872.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2093..3229)
                     /locus_tag="JN334_003907"
     CDS             complement(2093..3229)
                     /locus_tag="JN334_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY7439873.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3222..>5218)
                     /locus_tag="JN334_003908"
     CDS             complement(3222..>5218)
                     /locus_tag="JN334_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439874.1"
                     /translation="QTYFIDLPCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWD
                     HLFEDESQQTALPSALAHYFAQLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVV
                     CGGWHAPALAKMWRECPQVINTPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAP
                     VWQNWCWQWGLQQAGEQLLKTVLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPL
                     RTDWLDAIAGSLIKEALNAPLPWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQ
                     PPLPKDVTCELERTAISLPAELTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLT
                     LAGNGEERWKLTRPLSQHAALIEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQ
                     AASAGLASFSQQLLEQLTLLIAQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTT
                     LCAAIDRTLWLCESNGRPDEKEFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALL
                     ERHSQAIHVLALDRGATLGALMRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALAR
                     HQLACQPAFIAGFSSHLNQLSDADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLA
                     QLPVSALQMPLHCPPQAIAHHQQLEQQALASLQHWGVFHV"
BASE COUNT         1186 a         1442 c         1403 g         1187 t
ORIGIN      
        1 gtgactctca ccaggggcta tataggatat gcctaatacc gtggcgtgag cagtccggaa
       61 ctggagtaga actcttagta aaaagcacta tttcatcctt gttgctgaag catggggaat
      121 aattgttcgc aaagcaaaac accgttattc attgcttcta cccgtgcctc gctttctgta
      181 ttacgaaatt gtgccaacac atgtgccagc cgataaaaac ccaccgcggt gaggtcattc
      241 gccagcaact ctgcctgatt aatagcgctc tgttcctgat agcgccagcc gttatggagc
      301 agttgaataa gtaacgcctg gcagcgcatc agcaactgat gagcagtaga cggcacaggc
      361 agaacgctgg cagaaggtag tgataccacc acaggcgcag tttctgcgtc cagcgcccag
      421 gcacgagtct ttgtcatcat tacctgtggt tccagtgtca attgcccatc aacaaaactg
      481 acaaagccag aaaccagaca cacggggtcg tctgtttgtt gcaaaagcgc cgccatgcgt
      541 tcaatggcat aaggcgcgct ggctgaggct ggcagtgata acgtcagcag attatcttca
      601 ccttcgccgc tgattacctg cgcatccagc gtctggcggc tgctatccca accgagcgaa
      661 atacactcag cgaccggcag aataaataag ttatcgacct gattaagagg ccgtatgcag
      721 gcggggggac gctggcgtaa atattcccgc aaagccacaa tgcccggctg gcgtaacggc
      781 gcgctcaaca tttgccaggc atcaggcgac agcggtacaa cgctgcttaa gcggttgcgg
      841 gtagctaaca gcagctcgcc atcggcactg cgttttgctg cttgtgaaac aatttgccca
      901 cccgccagtg cgccagcctg aaaactaaac agccgacgcg tagctgccgg tgagtcttcc
      961 tgttcacttc gcggccaact gcgcgaaagg tgcaaaatac tgccggtgtc gggatcggta
     1021 aaccagatgc gtaaaccata atgctcaata tcctgccagc aacgcatacc taaagacacc
     1081 agccgcagat gatcaagctt tgcttctccg gcaatgccag agccaacgac cgtgcgccac
     1141 ggcacaggag gaacttcacc aacactgtcg ctccgggcca tctcttgtgc gcaatttaat
     1201 cgactgttta atgccgcaag ctgacgtaag cattctccgg catgatagtg gctggcgcgg
     1261 gcgtggaagg catcaacgct ggcgcgcagt tgccgtagcg attcactcac ccatcgccag
     1321 ctgcagcgtt ccgctgcttg ctgcgcgcgg ctgaaagcgg cctcgtagtg aataagcggc
     1381 tggctgatgc cgctcagcca taatgcctgg cttagttgtt gaacatattg acgacacgtt
     1441 ttgccttctt cgctggcaaa cggatcgtca gatgatgtga cgtgttcgct gcgcatctgc
     1501 cagattaaat gagtcaattc cgcttgctgc gcttttgcct ggacaaaggc ctgtactgcc
     1561 agtacgacat gttcgcaaag tgtgccttca atacaatcac aacgggcgaa acgaatactg
     1621 ctgcgggaat aaaaacgcac atcgctcatc ggtaagcggg cagagggaat ttcacccggc
     1681 gcacagaaca actcaatggt gatgctttta gcgaccagcg cctgtgcgcg tttgcgggtg
     1741 gcatcgggaa gggtagccag ttcttccagc cagattgccg gatcccattc ctcttctttt
     1801 cccgtaggct gggcggtagc acaaagtcgt tgataactta acaccagcat cacgcgatga
     1861 cggcacatgc cgctggcccc ataggtgcac tgagcctctt tcagtgcctg gccgttcgcc
     1921 agctgggtac ggacaccgtc actgaaggtg gcgattaaag cgctgttctc atggctgatt
     1981 tccgggacgt tgccattttc cagttcctta aggctgcgct taacaaaacc ggcattgctt
     2041 aacgccgtca gtgcctgcgg tgtcagttct aataattccg gacgtagtga attcatgact
     2101 gaagattctc cgcaagccat gatgccagct cgcccggtgt catggcggct atttgtgcgc
     2161 cgacattaac cagcgcctgg gccgtatcgc ggtcatagca aggtgttgct gtgctatcga
     2221 gcgctgccag tcccagcact ttgatgccgc tctggacaca ctttttcact tggtgcgtca
     2281 gcaatgatga tgaaccccct tcataaaaat cgctcacgag gataatgacg ctttttgcag
     2341 gttgttcaat aagttgccga ccatactcca tggcactggc gatattggtt ccgccgccta
     2401 attgcacttt cattaataac tctaccggat cggcaacgtc tgccgtgaga tcaacgacgc
     2461 ttgtgtcaaa cgccaccaga tgggtacgaa tgccgggtaa ctgccataaa caggccgcca
     2521 tcaccgcaga gtggatcacc gaatcgacca tcgatccgct ttgatcaacc agtaagacca
     2581 gttgccattg ctcgctttgg cgtttaatgc ggctgttaaa gcgggggaat tcgatataca
     2641 acttgccgtg ttgcgggtgc cagtgttgca ggttggcgcg cagagtactt ttgaaatcaa
     2701 agtttcgcgc cagtggaata aatgagcggc gacgacgatc gcggacacca gaaaaagcct
     2761 gacgaacttc ctttgccagt cgcgccataa tttcttccac aacctggcgc acaatcctgc
     2821 gggcggcagc cagtacttcg gggttcatca gatgtttggt gtgcaaaacg gcgcgtagca
     2881 ggctttcgga aggctgcata cgttccagca cgtcgagatt tgtcaccaca tcttcaatgc
     2941 cgtagcgcag tacggcatcg ctttccagcc gctcaatcac ctgttgcgga aacagcgtgt
     3001 gaatactgtt gatccactca ggagtggtga gatttgagcc acctaatcca ctagagcgtt
     3061 caccacgctg gagccgttca ggatcgcgcc catacagcca ctccagcgcg tgatctatct
     3121 gccgggcgtt gtcatccagc ccacaaagcg tcgtttctgc cgcttcgcca agaattaatc
     3181 gccagcgttg tagctcacgg gtggtcagaa gatcgttcag ttcagacatg gaaaactccc
     3241 cagtgttgca gcgatgccag tgcctgctgt tcgagttgtt gatgatgtgc aatggcttgc
     3301 ggtggacaat gcaacggcat ttgcagtgcc gaaacgggaa gttgcgccag ttgataatgc
     3361 tcaagcacct gatgcgccag cgtcccgcgt tctcgtggcg gtagccaggc cattgccgcg
     3421 cgtaaatcgg gcagggcatt ggtaaaatcg gcatcactta gttgatttaa atgactgctg
     3481 aaaccggcga taaatgccgg ctgacaggcc agttgatgac gggctaatgc cagcaaaccg
     3541 tgcagcgcct cgccggattg tgccggggat aactgcgcca gcatcgtcag cgccgcttcg
     3601 gcactagcgt tgggatgttc cagacgcatc agcgcaccaa gagtcgcgcc gcgatccagt
     3661 gccaggacat gaatcgcctg actgtgtcgc tccagcaagg ctaccgccgc agaaagcgaa
     3721 acgccgggga gttgcacacc gctatgtaga tcgcgcagga tatgacacaa cgcttgccag
     3781 ctatgcaagt gagcgtgaaa ctccttttca tccggtctgc cgttggattc acacagccac
     3841 agcgtgcgat cgatagccgc gcataacgtc gtttgtaata tctgcgcgcc ttgcataccg
     3901 ctaatttcat ccagccgcca taaggcatat agcacttcca gcgcctggcc catttcggca
     3961 aattgatttt cctgggcgat taataatgtg agttgctcca gtaattgttg actgaaggac
     4021 gccagacccg ctgacgccgc ttggctaaga catgtggtaa tgctgccgat tccacccgcg
     4081 tccagcatat cggcttctaa tttatggcgt gcggcttcca ggagtgtggc accaaagcag
     4141 gcggcctcaa tcaatgccgc atgctggcta agcgggcggg ttaatttcca gcgttcttca
     4201 ccgttgcctg caagtgtcag tgtgcttccc tgctggcgta caatccctgg gatctccagt
     4261 attgccagcc gatgtaacac ctgactttgc gctagtccat tggggttaaa gcgatttaag
     4321 gtaagctccg ccggaagaga gattgcggta cgttccagtt cgcaagtgac atcttttggc
     4381 agaggcggtt gtggcgtaga aggggcaagt ttaccgaatc cgtcacccgc taatgtgtct
     4441 atcaacgtta gcagaatcgg atcggtatcg ggatgaataa cgccgcgata gctccacggc
     4501 aacggcgcat tcagggcttc tttaatcagc gatcctgcta tggcatccag ccagtcagtg
     4561 cgtaacggta gtgtatgacc gcgcaactgt gccagtgcca tcgcatgcag atgagcggca
     4621 gccatatccg cggtcgaagc aggcaacttg tgctggcgca aacgggtgag aaccgttttt
     4681 agcagttgtt ctcccgcctg ctgtaagccc cactgccagc accagttttg ccagaccggg
     4741 gcaggcattc ctgaaaggta tcctgccagc acatcaaggc gcttttcact gtagggggtg
     4801 agataacaac ctgtaacggc atctgccagc gacggcaatt ctggcgtgtt aattacctgc
     4861 ggacattcgc gccacatttt tgccagtgcc ggagcgtgcc agccaccgca gacaactaac
     4921 acgtcgccat tattttgctg catcgcccat ccaatccagc gggccataaa ggcttcgcgc
     4981 tgacgattta gagcatcgcc gggggagtcg ccccgcagtt gggcaaaata gtgcgccagt
     5041 gcagagggta atgcagtttg ctggctttca tcttcgaaca agtgatccca cagggtgtcg
     5101 ctgttatcca tgcgggtggc acgcagcagc aaggtctggc tttcctcttg cgtatcaggc
     5161 gaatcgtcct cttcttcact ctgcgcccag caaggcaaat cgatgaagta agtttgtg
//
LOCUS       DADLTY010000237         5181 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.240, whole genome shotgun sequence.
ACCESSION   DADLTY010000237 DADLTY010000000
VERSION     DADLTY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.240"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            9..746
                     /locus_tag="JN334_003909"
     CDS             9..746
                     /locus_tag="JN334_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY7439875.1"
                     /translation="MSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNV
                     TYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLG
                     SGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSF
                     YLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGL
                     SATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="JN334_003910"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="JN334_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HAY7439876.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..3810
                     /gene="safA"
                     /locus_tag="JN334_003911"
     CDS             3607..3810
                     /gene="safA"
                     /locus_tag="JN334_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HAY7439877.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3878..4639
                     /gene="ydeO"
                     /locus_tag="JN334_003912"
     CDS             3878..4639
                     /gene="ydeO"
                     /locus_tag="JN334_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HAY7439878.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
BASE COUNT         1480 a         1102 c         1156 g         1443 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgga aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa
     3661 atcatgtctg taattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctggaaataa acagcaaatt ataaatgtga aatgtttaaa
     3841 aggtatcgat aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat
     3901 atttattcat catgctttca acgctaacat tttagataaa gattacgcct tctccgacgg
     3961 cgagatcctg atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt
     4021 taaagaaatc ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca
     4081 gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
     4141 gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga
     4201 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
     4261 aactaacggt gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc
     4321 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
     4381 gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca
     4441 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
     4501 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
     4561 gagagtttct aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat
     4621 gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga
     4681 attatatggg gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat
     4741 aaataagtga catccatcac atatttatgc acttgcataa cctgttgcat gattatttat
     4801 gatctcaatt cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag
     4861 ttgtttatcg gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact
     4921 gttatttccc tttttttagc taaatctgct atctctttat gtgactaact tcacttacat
     4981 ccacttattt ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt
     5041 atgaagtctg cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct
     5101 ggtatggctg catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca
     5161 aacgttgctt tctcagactt t
//
LOCUS       DADLTY010000238         5164 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.241, whole genome shotgun sequence.
ACCESSION   DADLTY010000238 DADLTY010000000
VERSION     DADLTY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.241"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..222
                     /locus_tag="JN334_003913"
     CDS             <1..222
                     /locus_tag="JN334_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7439879.1"
                     /translation="SLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFH
                     WGFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(394..723)
                     /locus_tag="JN334_003914"
     CDS             complement(394..723)
                     /locus_tag="JN334_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HAY7439880.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(728..913)
                     /locus_tag="JN334_003915"
     CDS             complement(728..913)
                     /locus_tag="JN334_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HAY7439881.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(910..3549)
                     /locus_tag="JN334_003916"
     CDS             complement(910..3549)
                     /locus_tag="JN334_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HAY7439882.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3757..4746
                     /gene="ldhA"
                     /locus_tag="JN334_003917"
     CDS             3757..4746
                     /gene="ldhA"
                     /locus_tag="JN334_003917"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY7439883.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4857..>5164
                     /gene="hslJ"
                     /locus_tag="JN334_003918"
     CDS             4857..>5164
                     /gene="hslJ"
                     /locus_tag="JN334_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HAY7439884.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     "
BASE COUNT         1308 a         1322 c         1204 g         1330 t
ORIGIN      
        1 agtttgtacc gaatgtttgc cgataaaggt ttggtagtcg cgcaatatat tcgtaaccgt
       61 cgtctcgatt tttgtgcaga tgcgattcgc catgccgcag atgatgaaaa actggcaggc
      121 atcggctttc attggggatt ttctgaccag agtcattttt caacggtatt taagcaacgc
      181 tttgggatga cgccaggcga gtatcgacgt aaattccgct aactaattgg cgttgcatgt
      241 actgcaacgc caattagtta aaacagatat ttgactatgc atttttcagg gcgaaaggca
      301 atttgacagg agtttttgaa tattcacctt gtttcaggaa aatagatcaa tcgctttctc
      361 ctgtagcgtt atcccgatat tgcggttttg ctattaaaac tttcgcaccc atttaccgtt
      421 tatcccttgt acatattccc ccggtttggc ccgagctacg agcttttgcc cggcgagttt
      481 cgcgatatca tctacagaca cattattttg ctttgcccgt tgttggtaac tcgctttacg
      541 ttcggcatta atttcggtta ctaatttctc tgtttcagca tccgttttca gcgcaaccag
      601 ataaccataa aatgtttcac ctacccgccc ctgagttctg gcttcatcca gagtcaatgc
      661 cattacattg ctgcttacca gcccaacaag aaacgcacaa agaagtaatg ttttcttcat
      721 catcgcctca gaaaagatcg ctacgagttt caagcagctc ttcgacatct ttgtctgcct
      781 tgatgatgat ctcatgctca attttaacgt tcatattgat agtgatcggt tccttaggtg
      841 cagcgacttc aatgcgaggc gtacagccaa ccagcataaa agatgacgtc aacgcagcca
      901 gtaaaatttt cattgttttt cctcacactc ttttccttgc ggacagtcat ttcccggcag
      961 acgtgcgttc tgctccaacc atgcctggag attatcgcca aagcgtaaac tgcgccacag
     1021 cgtaaaaata ttctcttcat gatggtaatt aaggtttacc gtaccgcttt taccatcaac
     1081 gcgactggta cctgtaatgt tggcctgcat ggttaataaa ccgagattat ctaagttaat
     1141 ttttgtcgat gaccggctaa tttccatata gcgcaaccag ttaattgctg aacccgcagt
     1201 catattgtct ttcaccaccg catctgcggt atctttatcc agccgcaagg tcattagccc
     1261 gctatttgcc agccaaccat ctttcactat ccatttttcg ttgtttagcc ataacggcaa
     1321 tgcaccacta aatgccccgg acatggcgaa ttgtttcgga ttgacggcgc taaccagttc
     1381 gctggatgag agattattca gccgcaacag cgccgggtca tgttgcggca tacgtaattg
     1441 tttcatcagc acgttaccgc ctaacacatc gacgctaaca tcagtcaaca gcaagggttc
     1501 ttcttcagtc cacggataac gcccttgcaa atcagccgta atatttttcg ctgtcaccag
     1561 attaatcact tcggcaattc gcaacgtaac ggggccgcga gtccccagat gccaggctcc
     1621 atcggcaaaa cggaaaggca ggacaaaatc gacaccgtta acctgattat ctggcatcca
     1681 ggcactaccg cctttcaaca cgccgtgccc tcccgcgcgg aatccttgtt caggcgcagc
     1741 agaaaatgca acctgagcat acagttcacc atcgcgtaag ttcatcttcc agtcgggtgg
     1801 caccagcggc tggaatacag tcagtgattg ttttggccac caggcgttac cgcgcagacg
     1861 aataccgtcc cagcgaccat ttacccgaac cgggccaatc tcaccagcat gtaaatcgcc
     1921 tttaaagagg aaataggtcg gattgcgccc atcgacgcta aattttaagg ttgatggcgg
     1981 taacacactg ccgccagtga acagcgtttg cccggcgtcc agtgacagcg cgccgctgaa
     2041 ggagggatgc tgtgcgtcac gtacccagac gacgggcttg tcgagaatta atcgcggctt
     2101 ttctaccgtc atcgtaccgt attgtaactg gtcgaaaccg gtggagagat cggtcagcgt
     2161 aatcgtgctg tcatgccact caccttttcc tgcgacatcc caggtggcat tcatcggtgt
     2221 aaaactccct tttccccagt agcgccactg ccagcggcca gcgtcaggga gaaaatcatt
     2281 cgccagccca tccatatgca gcacgaaatc gcccagttca ttttcatgcg cctgcaaaat
     2341 ggcctgcaaa cgtccgtcaa caccacgttg ggtgactttt acacccgcta aaggccagcg
     2401 gatttcatcg atgtccagcg aatcgatgac tcttcccttt gaacgaagta acgcgcctgg
     2461 ctcaaaggtc agcgttgggt cagacagact tccacttagc tgcgcaggta aacgggcata
     2521 taaaatgagg tccgcctgtt tcgcttcacc ggtcagctgc agaggcagtt gactgttatc
     2581 catgcttaat tttcctgggc caaaattaag caccgcgttg cccttacccg cttgcccctg
     2641 ggtcagcaca ttcagtcgtc cgctgaccag agcgttctca agccctgcct gccagttgtc
     2701 gactttgaca cccagtcggc cactcaaagg aaaaccagca tacagccagc tccagcgacc
     2761 atcgcttacg gtcaattgtt gacgagtaat ttgccacggc aaatcgagca acggatcgcc
     2821 gttatcccgt gccagcacga tcaattgccc gctattttcc tgccagtcca gctcggcatc
     2881 caccagtgac ggttcctgcg gcaagtttaa cgtcgcagta gcatgcccac ttacaggaag
     2941 tccatccggc acgagcggca tagtaaattc ccccaccagt ttaaccggcg gctgattttc
     3001 aaacgcgacg acatccagtt cgctgactgt aagttgttgc cctttcagct ggccttgaaa
     3061 tttaactttt tcgccctgat aacgcagttg ctggatatca gaggttaatg cgagagagag
     3121 ttttccctgc cattcctgcc agggagaaaa aatcagttta tcgatattga tccaggtgtt
     3181 aggcagcatg gactgccact gcgcgagggt ttttggagca gctggcgatt gctccgtctg
     3241 cggcaatttc gccagacaag cagaatcaag ttctaccgtg ccgacgttca ataaccagcg
     3301 gctgggatgt gaaaggctgg cgttggtgat atgcgcaagc tgacaatctc ccaccagata
     3361 acggagatcg ggaatgatta aacctttacg cgtaatgcgt gggctttcat ctaatgcaat
     3421 acgtgtcccg agcggtagcc agatgtccgc cagcgtggga acccacagcc cgagcgtcat
     3481 cagcagcgtc aacggcacaa gaataatcag taataacagc gcgagaacgg ctttatattt
     3541 acccagcatg ggtagttaat atcctgattt agcgaaaaat taagcattca atacgggtat
     3601 tgtggcatgt ttaaccgttc agttgaaggt tgcgcctaca ctaagcatag ttgttgatga
     3661 atttttcaat atcgccatgg ctttcaatta tatttgaaat tttgtaaaat atttttagta
     3721 gcttaaatgt gattcaacat cactggagaa agtcttatga aactcgccgt ttatagcaca
     3781 aaacagtacg acaagaagta cctgcaacag gtgaacgagt cctttggctt tgagctggaa
     3841 ttttttgact ttctgctgac ggaaaaaacc gctaaaactg ccaatggctg cgaagcggta
     3901 tgtattttcg taaacgatga cggcagccac ccggtgctgg aagagctgaa aaagcacggc
     3961 gttaaatata tcgccctgcg ctgtgccggt ttcaataacg tcgaccttga tgcggcaaaa
     4021 gaactggggc tgaaagtagt ccgtgttcca gcctatgatc cagaggccgt tgctgaacac
     4081 gccatcggta tgatgatgac gctgaaccgc cgtattcacc gcgcgtatca gcgtacccgt
     4141 gacgctaact tctctctgga aggtctgacc ggctttacta tgtatggcaa aacggcaggc
     4201 gttatcggta ccggtaaaat cggtgtggcg atgctgcgca ttctgaaagg ttttggtatg
     4261 cgtctgctgg cgttcgatcc gtatccaagt gcagcggcgc tggaactcgg tgtggagtat
     4321 gtcgatctgc caaccctgtt ctctgaatca gacgttatct ctctgcactg cccgctgaca
     4381 ccggaaaact accatctgtt gaacgaagcc gccttcgatc agatgaaaaa tggcgtgatg
     4441 atcgtcaata ccagtcgcgg tgcattgatt gattctcagg cggcaattga agcgctgaaa
     4501 aatcagaaaa ttggttcgtt gggtatggac gtctatgaga acgaacgcga tctgttcttt
     4561 gaagataaat ccaacgacgt gatccaggat gacgtattcc gtcgcctgtc tgcctgccac
     4621 aacgtgctgt ttaccgggca ccaggcattc ctgacagcag aagctctgac cagtatttct
     4681 cagactacgc tgcaaaactt aagcaatctg gaaaaaggtg aaacctgccc gaacgaactg
     4741 gtttaatctt gcagctcccc tgcaatccag gggagctgat tcagataatc cccaatgacc
     4801 tttcattctc tattcttaaa atagtcctga gtcagaaact gtaattgaga accacaatga
     4861 agaaagtagc cgcgtttgtt gcgctaagcc tgctgatggc gggatgtgta agtaatgaca
     4921 aaattgctgt tacgccagaa cagctacagc atcatcgctt tgtgctggaa agcgtaaacg
     4981 gtaagcccgt gaccagcgat aaaaatccgc cagaaatcag ctttggtgaa aaaatgatga
     5041 tttccggcag catgtgtaac cgctttagcg gtgaaggcaa actgtctaat ggtgaactga
     5101 cagccaaagg gctggcaatg acccgtatga tgtgcgctaa cccgcagctt aatgaactcg
     5161 ataa
//
LOCUS       DADLTY010000239         5151 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.242, whole genome shotgun sequence.
ACCESSION   DADLTY010000239 DADLTY010000000
VERSION     DADLTY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.242"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            190..447
                     /locus_tag="JN334_003919"
                     /pseudo
     CDS             190..447
                     /locus_tag="JN334_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="JN334_003920"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="JN334_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HAY7439885.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /locus_tag="JN334_003921"
     CDS             complement(1599..2351)
                     /locus_tag="JN334_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HAY7439886.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="JN334_003922"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="JN334_003922"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAY7439887.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="JN334_003923"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="JN334_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3605..4420)
                     /locus_tag="JN334_003924"
     CDS             complement(3605..4420)
                     /locus_tag="JN334_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7439888.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4517..>5151
                     /locus_tag="JN334_003925"
     CDS             4517..>5151
                     /locus_tag="JN334_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7439889.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1374 a         1175 c         1226 g         1376 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagcagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctggcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg gaatataccc t
//
LOCUS       DADLTY010000240         5047 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.243, whole genome shotgun sequence.
ACCESSION   DADLTY010000240 DADLTY010000000
VERSION     DADLTY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.243"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(26..214)
                     /locus_tag="JN334_003926"
     CDS             complement(26..214)
                     /locus_tag="JN334_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY7439890.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="JN334_003927"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="JN334_003927"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY7439891.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="JN334_003928"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="JN334_003928"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY7439892.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /locus_tag="JN334_003929"
     CDS             3536..4270
                     /locus_tag="JN334_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY7439893.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="JN334_003930"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="JN334_003930"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY7439894.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="JN334_003931"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="JN334_003931"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY7439895.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
BASE COUNT         1274 a         1300 c         1315 g         1158 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaac
//
LOCUS       DADLTY010000241         4991 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.244, whole genome shotgun sequence.
ACCESSION   DADLTY010000241 DADLTY010000000
VERSION     DADLTY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.244"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(38..1132)
                     /gene="yaiW"
                     /locus_tag="JN334_003932"
     CDS             complement(38..1132)
                     /gene="yaiW"
                     /locus_tag="JN334_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HAY7439896.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(1145..2365)
                     /gene="sbmA"
                     /locus_tag="JN334_003933"
     CDS             complement(1145..2365)
                     /gene="sbmA"
                     /locus_tag="JN334_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HAY7439897.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            2717..3874
                     /gene="ampH"
                     /locus_tag="JN334_003934"
     CDS             2717..3874
                     /gene="ampH"
                     /locus_tag="JN334_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HAY7439898.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(3875..4378)
                     /locus_tag="JN334_003935"
     CDS             complement(3875..4378)
                     /locus_tag="JN334_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7439899.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(4466..>4991)
                     /locus_tag="JN334_003936"
     CDS             complement(4466..>4991)
                     /locus_tag="JN334_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7439900.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         1264 a         1311 c         1196 g         1220 t
ORIGIN      
        1 cctgaaacgc cttatctggc ctgacattcc tgtttgatca ctgtttcata caacgcgccc
       61 gccgttcgtc tacgcgcttc gcaaaccagg ccgtagtcag gttgcgggtg attttcgggc
      121 tttccagttg aatgccaggc aacatttcgc ggggtagtga tttacccgtt ttcgcttcgg
      181 caagttgata aactttcttg tacagcgccg tctcctcaaa agagaagctg tcacctttct
      241 ctaactgacg gcgaatttcg ctgtcgctca tccccagttt tcccgccagt ttgcgcgttg
      301 ccagttcggt tttcccgggt tctttactgt cgtaacgaat taaatcaccg tccagtgcca
      361 gcttcacgcc gctggcctta ctgactgcgt tctgaaacgc ggcattacgg ctggcgtacc
      421 agccagcgtt aaaatcagca aagcggtata tcggtgcgct atagctggcg ggataattca
      481 gtaaatggta agtaccaaac cacaacccgc cgcggcgact gaagacttcc tgacgaactg
      541 taccgtccat tttccacgga taccctttgg tatgctgttc ggcaaaagca atgctgacct
      601 gcatcggccc accggtgcgc accggattaa gcgaaccaaa cagcgtctgc cccattggta
      661 ccatgctgat cagatcgtca aaaatcgcgc tcaattgctt ttctgtacgc accgaatcta
      721 accgttcgct ataacttttg ccgtttggcg atttgatttt tagcgcggta tgaaccagaa
      781 aagcaggaat gtgcatccgt tcagcacgac ggtcaatctc ttgccaggcg atcttgctta
      841 agcccggaac ggccggatct gcctgataat ttgactcctg ctgcgccacc gccagcaccg
      901 agtagacgtt ttccagcgtc ggtgccagcc cctgactttc aaaggtggtg gcgagatctt
      961 tcgcccaggc atcgcggtct ttcacgctcg caggcatctt ctgccgcacg acgctcgcta
     1021 catctatcgc tttttcgccc tttttcaacg gttgcggagc ctgactacta caccccgcta
     1081 aaaccagaac cgccaacaat gacagcgaag atagatgatt gacgcgcgac attacttctc
     1141 ctttttagct caaggtatgg gttacttcct gaattttgtc accatccagc tcatgttcaa
     1201 agctgcgcag acgtttgtag atagacatca actcaaccag tgtggtccat gagttaatca
     1261 ggtactggaa agcaccgcga acctgaccaa aaacgttggt aatctgcgtc atcaggccga
     1321 gcgtaatcgt accggcaaca attgacggaa acagcaagaa caaaccgaaa acgttatcga
     1381 cctgcaaata gagaatgcgg gcgatgttga aatacatata gtgaaaatag aggcgaaaat
     1441 agtttttccg tacggcgcta aacagctcgc gtaccgtagg cggcgtcgcg cgcgtggcat
     1501 cgtcttcacc ataaaccagc tctttacggt aggcggcctc tactcgctgg tttttaaact
     1561 ccagccccgg cagtttgatc cctaccactg ccagcaatcc ggtccccatc agcgaccaga
     1621 cgatagcggc aatcaccaga ccatacggaa tgtgcccgat aatcggcagc tccggcacat
     1681 gcgcggagag cgttaccagc accggcagga aggcgatcaa cgtcatgatg gcgttgataa
     1741 aactgacgcc catattctcc agcgttgaag caaaacgcat ggtgtcttcc tgcacacgct
     1801 gtgcggcacc ttcgatatga cgcagttgtt gccagttcgc catgtaatat tcgttcatcg
     1861 ccgtacgcca gcggaacacg tagtgactga caaagaagtt gttcagtaca ctgatcacca
     1921 ccgcgatcag cgcaatcccc agaaagacgc ccacttcgcg gtaaaattgt tcgatagtga
     1981 ctttatgcgg cgaacttagc gcggtttgaa tcagatcata gaacggcgca taccaggcgt
     2041 tgacggcgac cccgacttcc accaaaaacc aagtgacgaa gataatcagc gcagtaccga
     2101 gtattgacca gtattgccaa cggtgcgggc tgtagataaa ccagaaaaat gcaaaaagtc
     2161 ctacgcaaac aatgtagtaa gcgtaaaaaa tcaggaaatc caacgaccag aaacgcgcgg
     2221 cgctaatcgg gatctgcccg gaagcgccgg tgatacgtgc cacccagtca cccccaccgg
     2281 cttgccagaa gataacggca atcaatgccc aaacaaaggc cgagagaaaa aacgttcccg
     2341 gctttgggaa aaaagactta aacatatgca ctcctgctaa cttcttatcg ttttaacaac
     2401 tgctgtgtat taaccgcatg accgctacaa agcgtcagcc aaatgaaacc gcccattgtg
     2461 acccatagca aggcgcttag ttcgccagcc tgaccataag aattgtttcg acggatttca
     2521 caaaccaaag attgtttaca taagcggatt aacacgcgca ggatcacaaa tgcaggcatt
     2581 ggtgagcagg aaaaagatcg tgtttacgcg ccgttacgtt tttaacacta ttattgcact
     2641 aattcgccct ttgcaatcca tcaatgagta gtataaatac gctcagttac cttcattcaa
     2701 tctatggaca ccaccgttga aacgtagtct gcttttttct gccgtgctgt gtgcggcgtc
     2761 attgacctcc gtccatgcgg cgcagccgat cactgaaccg gagtttgcct ctgatattgt
     2821 cgatcgttat gccgatcata ttttttacgg cagcggtgcc acggggatgg cgctggttgt
     2881 tatcgacggt aaccagcgcg tctttcgtag ttatggtgaa acgcgacctg gtaataacgt
     2941 tcgcccgcag ctggattcgg tcgtgcgtat tgcttccctc accaaactaa tgaccagtga
     3001 aatgctggtg aaattgctcg accaggggac cgtaaagtta aacgatccgc taagtaaata
     3061 tgctccgcca ggcgcacgcg tgccgaccta caatgggacg cccattaccc tagtgaatct
     3121 ggcaacccat accagcgccc tgccccgtga acagcccggt ggcgcggcac atcgtccggt
     3181 gtttgtctgg ccaacgcgcg agcaacgctg gaaatacctt tctacggcga agctgaaagc
     3241 cgcgccaggc agtcaggcgg cgtactctaa ccttgcgttc gacctgctgg cagatgcact
     3301 ggcgaatgcg tccggcaagc cttataccca gttgtttgaa gagcagatta cccatccatt
     3361 ggggatgaaa gacaccacct acaccccctc accggatcag tgccgccgtt taatggttgc
     3421 tgagcgcggt gccagtccgt gcaataacac gctggcggca attggcagcg gcggcgtcta
     3481 ttccacgcct ggcgatatga tgcgctggat gcagcagtat ttgtcatctg atttttatca
     3541 gcgcagtaac caggcagatc gcatgcagac gttgatttat cagcgcgcgc agtttacgaa
     3601 agtgattggc atggatgtcc ccggcaaagc ggatgcgctt ggtttaggct gggtatatat
     3661 ggccccgaaa gagggtcgtc cgaggattat tcagaagaca ggcggtggtg gcggtttcat
     3721 tacctatatg gcgatgatcc cgcagaagaa tattggcgcg tttgtggtgg tcactcgttc
     3781 gccgttgacg cgctttaaaa atatgagcga tggcatcaat gatctggtga ccgagctaag
     3841 cgggaataaa ccgttggtta tccccgcatc ctgattaata ctctgaaggc aaacggttga
     3901 tagcgaccag tttgccttta ttcatttcga tatagccgcc tttacgcaaa gcagcgagaa
     3961 cttctgcgac gacagaacgc gatatgcgtg tacgttgatg gatataattc atcacaccaa
     4021 tacgtgatcg caattcttca ttccagtcaa tcattgataa taatgttgcg cgggtttgtt
     4081 tggctggtag atgatatccc gtacaatttc cttctgatat taatttgtat ggtatatcgt
     4141 ttttcattaa accatcagcc agccccataa tataaggagc ctgggtaata ccgataagta
     4201 cgttttcttc tctacgcaga gagataactc cttctaaaat aacgaaagta tcttcgttat
     4261 tgacatcact ggaaaatata acttgctttt cattattaaa ctcgaagcgc gtaccgtatc
     4321 tggacaaaca tttatcgagc ttaccaaatt cctgaagagg tttaactaca gataacattt
     4381 gcgcgtcctt tgcagtaatg cccgtcaaat ccttgacggg cattatttag attaaattac
     4441 cagtatttct tcggagtgaa gaatattacc aagtatattt aacacccacg ttcgcggacc
     4501 agtcttgatc tacgtcacca ccaccgaggt agttagcatc ggtataggcg ctgaagttct
     4561 tggtgaagct gaactgagta cccagaccaa cacgtaccgc agacccttca gtaccgttat
     4621 cgatggaatc accgttcaca tcgttatcgt tgttagagtc gtcgtagacg taagccagtt
     4681 tgaagtacgg agtcagagcc tgatcttcgc tgtaggtgaa ggtataacct gcatctacac
     4741 ccagttcata acgcatgctg tcgtaaggct gaccgtcaac tttcatgtcg ttgctcagct
     4801 ggtagtcatc accagactgg aacagaccag aaatgctgcc gtaaggagtc acgtaaccag
     4861 catcacccag tttgaagtcg taaccggttt tcaaaccgaa gccccaagcg tcggagttgg
     4921 tgctaccgtc aacgtaagta ccgttgctca tggttgcaga caggtcgttg ttgaagtgag
     4981 agtagctcaa g
//
LOCUS       DADLTY010000242         4973 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.245, whole genome shotgun sequence.
ACCESSION   DADLTY010000242 DADLTY010000000
VERSION     DADLTY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.245"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(186..872)
                     /gene="kptA"
                     /locus_tag="JN334_003937"
     CDS             complement(186..872)
                     /gene="kptA"
                     /locus_tag="JN334_003937"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HAY7439901.1"
                     /translation="MWKRFCAGLSCRKFINPVTMTLVNKNRKSRDENAMAKYNEKELA
                     DTSKFLSFVLRHKPEAIGIVLDREGWADINKLILCAQKAGKRLTRALLDTVVATSDKK
                     RFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQFLYHGTASRFLDEIKKQGLIAGDRH
                     YVHLSADEATARKVGARHGSPVVLTVKAQEMAKRGIPFWQAENGVWLTSTVAVEFLEW
                     SFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="JN334_003938"
     CDS             1020..1703
                     /locus_tag="JN334_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HAY7439902.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="JN334_003939"
     CDS             1700..2161
                     /locus_tag="JN334_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439903.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="JN334_003940"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="JN334_003940"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY7439904.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="JN334_003941"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="JN334_003941"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAY7439905.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="JN334_003942"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="JN334_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HAY7439906.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /locus_tag="JN334_003943"
     CDS             complement(4704..4787)
                     /locus_tag="JN334_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide"
                     /protein_id="HAY7439907.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1176 a         1305 c         1239 g         1253 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DADLTY010000243         4945 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.246, whole genome shotgun sequence.
ACCESSION   DADLTY010000243 DADLTY010000000
VERSION     DADLTY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.246"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(217..435)
                     /locus_tag="JN334_003944"
     CDS             complement(217..435)
                     /locus_tag="JN334_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439908.1"
                     /translation="MFKCYCTLIVGGIMKNALQFLFVAFWLFASCMPIIFTARYMEKI
                     DVLILMFGHINALFLGVFMAVMCIEYWR"
     gene            complement(609..944)
                     /locus_tag="JN334_003945"
     CDS             complement(609..944)
                     /locus_tag="JN334_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439909.1"
                     /translation="MARVEGELFKIARASLEAEPIAWRYRYVKKGVMDSQGELWVGDW
                     KYVPKKEDCNDRPNYEIQALFTAPPVPVTSEELVKAVHFYEQLKRENPPASGNQINGL
                     TMSVKRPAN"
     gene            1052..1192
                     /locus_tag="JN334_003946"
                     /pseudo
     CDS             1052..1192
                     /locus_tag="JN334_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1192..2217
                     /locus_tag="JN334_003947"
     CDS             1192..2217
                     /locus_tag="JN334_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7439910.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2270..2359)
                     /locus_tag="JN334_003948"
     tRNA            complement(2270..2359)
                     /locus_tag="JN334_003948"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2323..2325),aa:Ser,seq:cga)
     gene            2453..3250
                     /gene="mtfA"
                     /locus_tag="JN334_003949"
     CDS             2453..3250
                     /gene="mtfA"
                     /locus_tag="JN334_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HAY7439911.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3351..3426
                     /locus_tag="JN334_003950"
     tRNA            3351..3426
                     /locus_tag="JN334_003950"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3384..3386,aa:Asn,seq:gtt)
     gene            3588..4850
                     /locus_tag="JN334_003951"
                     /pseudo
     CDS             3588..4850
                     /locus_tag="JN334_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1373 a         1161 c         1108 g         1303 t
ORIGIN      
        1 catgtgttca gcatggattg agcctcagat taatgttaag cgtcttgcag gacgcgtaat
       61 gttaactggg gcttttctct atctgccttt tggtgttcat gcctgagaca gatagcctca
      121 agcacccgca gtcattctac ttaactaaga tttccctgca aaccgttttt gtccggcaca
      181 gtaaatatcc aactaaacca atagcgttcg ctgtatttac cgccagtatt caatgcacat
      241 gaccgccatg aacaccccta aaaaaagggc atttatatgt ccaaacatta atatcaaaac
      301 atcaattttt tccatatacc ttgctgtgaa gatgatgggc atacatgatg cgaacaacca
      361 gaacgcaaca aacaaaaact gcaatgcgtt tttcattatt ccccctacaa tcaatgtgca
      421 ataacattta aacacacctc aatttggccg gacatataaa tatctaaacc agaaaaaatc
      481 acttacatag cgttacaaac tctttagtct aaatactcat cgtaaaacat tccccatact
      541 tatcagtccg ttccgcgcca ggtagcttat tgccttatct ggcaacctat aatcaggttt
      601 ccgctttttc agttggctgg tcgtttaacc gacatagtta acccattaat ctggttgccg
      661 gatgctggtg gattttcgcg ttttaattgt tcataaaagt gcacagcttt aaccagttct
      721 tctgatgtaa ccgggactgg tggggcagtg aataaggcct gaatttcata gttcggcctg
      781 tcgttacaat cctctttttt cggtacatat ttccagtcac caacccacaa ctccccctga
      841 gagtccataa cacctttttt cacgtagcga tatcgccacg ctatcggctc tgcttccagc
      901 gatgcccgtg caattttgaa taactcgccc tctactcgcg ccatccctga attggggtgg
      961 catttcgtaa tcgctatttt taatttggct tcttcgatta attgttcttt tgttaattca
     1021 gtcattttca ttaccgccct ggtaatgctg ccaccagcga aaatggttaa tggaaaacgg
     1081 gcttccgttc tataccaatc gtagtggcaa accgattgtc agccgggatc tatttacctg
     1141 caataaaact ttaccaccac gcgaggtaga gccgaatttt ggtgcgatct gatgggaaga
     1201 cgaaggaaaa atcctgaaca cgaaaaatta cctccaaatg tatacccaaa taaatatagt
     1261 tatgtatgga aaccaacatc cagagaatct gtaacactaa ccgccatcaa ggatggttta
     1321 gctgctttat ggaaaaagta tgaggaaact gtaaataatc gcgatcgtgc aatgacattc
     1381 ggtcgcttgt gggaaaaatt cctcgccagc gcctattaca gtgacctcag tccaagaaca
     1441 caaaaagatt atctgcaaca tcaaaaaaag ttgcttgccg tattcggtaa ggtgccggca
     1501 gattccataa aaccagaaca catccgtcga tacatggaca agagagggga acagagtaaa
     1561 acgcaagcca accatgaaaa aagcagtatg tcccgcgttt acagttgggg gtatgagcga
     1621 gggtacgtga aggctaaccc atgtgcaggt gtaagtaaat tcaaggccaa aaaccgcgaa
     1681 cgatatgtaa ccgacaaaga ataccaggca gtattaagcg ttgcacctct tcctgttttt
     1741 atcgcaatgg aaattgccta tctgtgtgca gcgagggttt ccgatgtgtt atcgctgaaa
     1801 tgggagcaga ttggaaacga cgggatcttt atccagcaag ggaaaacagg aaaaaaacag
     1861 ataaaagcat ggagtccacg attacaggcg gcgatcgaaa aagcaaaaca gttaccaaca
     1921 tccgcctatg taatcagcaa tcaatacggc aaccgatata tgtacaaagg ctttaacgaa
     1981 atgtgggtag aagcaagaaa tcgcgcaggc aaaatttcag gtattttaac cgacttcacc
     2041 tttcatgatc tgaaggcgaa aggaatttca gactatgaag gaagcagtcg ggataagcaa
     2101 cttttctctg gtcacaaaac cgaggggcaa gtgctaatct atgacaggaa ggttaaagtt
     2161 tcaccgacac ttgatgtccc gttacctgaa aatattccaa gaaaatattc caagtaattc
     2221 caagtgtgat ttttgtcact gacttaatga tgtataagtg attgaatttt ggcggagaga
     2281 gggggatttg aacccccggt ggagttgccc ccactccggt tttcgagacc ggtccgttca
     2341 gccgctccgg catctctccg ttcagatggt tgccatgatg ccaggaaatt tggcatttta
     2401 acagtccctg tccgtgcaat tttgttcaag tgacgagttt gcgagcaaaa cgatgattaa
     2461 gtggccctgg aaagtacaag aatcagcaca tcaaactgcc cttccctggc aggaagcact
     2521 atcgatcccc cttttaacgt gtctgacaga acaggaacaa agcaaattgg tcgctcttgc
     2581 cgaacgtttt ttacagcaaa aacggcttgt tcctttacag ggctttgagc tgaattcatt
     2641 aagaagctgc cggatagcac ttctattttg cctgcccgtt ctggagttag gactggaatg
     2701 gctggatggt tttcatgaag tcttaattta tcctgcgcca tttgtggtcg atgatgaatg
     2761 ggaagacgat atcggtctgg tgcataacca acgtattgtt cagtcaggtc agagctggca
     2821 gcaagggcct atcgttttga actggttgga tatacaagat tcttttgatg cttctggttt
     2881 taacctgatt attcatgaag tcgctcataa gctggacacc cgtaacggcg atcgcgccag
     2941 cggagttccc tttatttcgt tgcgtgaggt tgctggctgg gaacacgatc ttcatgctgc
     3001 aatgaacaac attcaggaag aaatcgaatt agttggtgag aatgcggcga gcattgatgc
     3061 ttatgctgcc agtgatcctg ctgaatgttt tgccgtactt tctgaatatt tctttagcgc
     3121 cccagaactt tttgctcctc gtttcccttc attgtggcaa cgtttctgtc aattttatca
     3181 acaagatcct ttgcagagac tgcatcacgc taatgataca gactcgtttt cagcgacgaa
     3241 tgttcattaa ttaacaactt tgaagattaa ttaaccaatt gaaatggctt atgaaattta
     3301 gtgttgacag acaaggtacc gctaagtaat atgcgccccg ttctcacgat tcctctgtag
     3361 ttcagtcggt agaacggcgg actgttaatc cgtatgtcac tggttcgagt ccagtcagag
     3421 gagccaattt tctgttttca tgcatccttg cgaatcttta tctgatgttg attcaacagg
     3481 ttagtgtgaa aactcttctc ggtgcatttt gattttaccc tacgcatcgg gaaaaattgg
     3541 tggtcaaatc tgggatcagg ttagttcgat aatggagtga cccccatata tcccttaccg
     3601 acgcaaaaat ccgcaccctc aagccttctg ataaaccctt taaagtctcc gattctcacg
     3661 gtctgtatct gctggtcaag ccgggtggct cccgccactg gtatctcaaa taccgtatta
     3721 gcggtaaaga atcccgcatt gcgctgggtg cctatccagc catctccctg tctgatgcgc
     3781 gacagcaacg tgaaggtatc cgtaaaatgc tggcgctgaa tatcaacccg gtacagcagc
     3841 gggctgctga acgtggctca cgaacaccgg agaaagtttt taaaaacatg gcgctggcgt
     3901 gacataaaag taacaggaaa tggtcgcaga acaccgccga ccgtctgctt gccagcctga
     3961 acaatcacat ctttccggtc atcgggaacc tacctgtatc agaacttaaa ccccgtcatt
     4021 tcattgacct gctgaaaggg atcgaggaaa aaggtctgct ggaggttgcg tcccgcacac
     4081 ggcagcacct gagtaacata atgcgccatg cggtccatca ggagttaatc gatacgaacc
     4141 ctgcagcaaa ccttggcggc gtgaccacac ctcctgtcag acggcactat cctgccctgc
     4201 cgctggagcg gctgcctgaa ctgcttgaac gtattggggc atatcatcag ggccgtgaac
     4261 tgacccggca tgccgttctg ctgatgctgc atgtgttcat tcgctccagt gaactgcgtt
     4321 tcgcccgctg gtcagagatt gatttcacaa accgagtctg gacgataccc gcgacgcgag
     4381 aacccattat tggcgtgcgt tattccggcc gcggggcaaa aatgcgaatg ccgcatatcg
     4441 tccccctctc agaacagtcc atcgccattc tgaaacagat taaggatatc accggtaata
     4501 atgaactgat cttccccggc gaccataacc cgtataagcc aatgtgtgaa aacacggtca
     4561 ataaggcact gcgggtgatg ggttacgaca cgaaaaagga tatctgcggt cacggcttcc
     4621 gggcaatggc atgcagtgcg ctgatggaat cgggtttatg ggcaaaggac gcagtagaac
     4681 gccagatgag tcatcaggag cgcaataccg tgcgcatggc ttatattcat aaggcagagc
     4741 acctggaagc ccgcaaagcg atgatgcagt ggtggtcgga ttatctggaa gcatgccgag
     4801 aatcttatgc accgccttat acaattggta aaaataagtt tatcccatag tatactaacg
     4861 aatcccacac tctggcaaat taccaccacc tctacaggag gtggttctat atagactttt
     4921 atcaacttca tgacgaaaaa cacct
//
LOCUS       DADLTY010000244         4839 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.247, whole genome shotgun sequence.
ACCESSION   DADLTY010000244 DADLTY010000000
VERSION     DADLTY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.247"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            1..660
                     /locus_tag="JN334_003952"
                     /pseudo
     CDS             1..660
                     /locus_tag="JN334_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            683..2194
                     /locus_tag="JN334_003953"
     CDS             683..2194
                     /locus_tag="JN334_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7439912.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2219..3217
                     /locus_tag="JN334_003954"
     CDS             2219..3217
                     /locus_tag="JN334_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7439913.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3250..4344
                     /locus_tag="JN334_003955"
     CDS             3250..4344
                     /locus_tag="JN334_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY7439914.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            4356..>4839
                     /locus_tag="JN334_003956"
     CDS             4356..>4839
                     /locus_tag="JN334_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAY7439915.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     A"
BASE COUNT         1067 a         1139 c         1464 g         1169 t
ORIGIN      
        1 cagtgaagcg ggtattccgg tgatttgcta caacacctgt attaatcaaa agggtgtcga
       61 taaatatgtc tcggcgtatc tggtcggcga tccactggaa tttggtaaaa actgggtaac
      121 gctgccgccg attattttat tgccaataaa attgaccagc cgaaaattgc cgtcatcaat
      181 tgcgaagcct ttgaagtttg tgtgcagcga cgtaaaggat ttgaagaagt attaaaatcc
      241 cgcgttcccg gcgcgcaaat tgtcgctaac caggaaggga ctgttttaga taaagcgatt
      301 tccgttggtg aaaaactgat tatctccacg ccggatctca acgccattat gggggagtcg
      361 ggcggtgcga cactcggcgc ggtaaaagcg gtacgtaatc aaaatcaggc cggaaaaatt
      421 gctgttttcg gttcggatat gacaaccgaa attgctcagg agctggaaaa caatcaggtg
      481 ctgaaagcgg tagtggatat ttccggtaag aaaatgggca atgctgtttt cgcgcaaaca
      541 ttgaaggtta tcaataaaca agccgacggt gaaaaagtga ttcaggtgcc aatcgatctc
      601 tataccaaaa cggaagatgg taaacagtgg ctggcaacgc acgttgatgg tctgccctaa
      661 tcgctgattc gcagaggtcc ctatgttcac ggcaacagag gcagtcccgg tagcaaaagt
      721 ggtggcagga aataagcgtt accccggcgt cgttgcgctg gataacgtta acttcacgct
      781 caataaaggc gaagttcgtg cgctgttagg taaaaacggc gcgggcaaat cgactctcat
      841 tcgaatgctt accggcagcg aacgcccgga tagcggtgat atctggattg gcgagacgcg
      901 actggaaggt gacgaagcta cgttgactcg ccgtgccgct gaactggggg ttcgtgcggt
      961 ttatcaggaa ttaagtctgg tggaagggct gacggtggca gaaaacctct gccttggtca
     1021 atggcctcgc cgcaacggca tgattgatta cctgcaaatg gcgcaggatg cccaacgttg
     1081 cttacaggcg ctgggcgttg acgttagtcc tgaacaactt gtttcaacgc taagcccggc
     1141 gcaaaagcaa ctggtggaaa ttgcacgggt gatgaagggc gagccgcgcg tggtcattct
     1201 tgatgaaccc accagctcgc ttgccagtgc agaagttgaa ctggtgatca gtgcggtgaa
     1261 aaagatgtcg gcactgggcg tggcggtgat ttatgtcagc caccggatgg aagaaatccg
     1321 ccgcattgcc tcctatgcca ccgttatgcg cgatggtcag gtggcgggcg atgtgatgct
     1381 cgaaaacacc tccacgcatc atattgtgtc gctaatgcta gggcgtgatc acgttgatat
     1441 tgcgccggtt gcccctcagg aaattgtgga tcaggccgta ctggaagtcc gtgcgttacg
     1501 ccataagccc aagctggagg atatcagctt tacgctacgt cgtggcgaag tgcttggcat
     1561 tgctggcctg ctgggggcag ggcgcagtga attgctgaag gcgattgttg ggctggagac
     1621 gtatgaacag ggcgaaattg ttatcaacgg cgagaaaatc atgcgccccg attacggcga
     1681 catgctgaaa cgcggcattg gctatacgcc agaaaaccgc aaagaagcgg ggatcattcc
     1741 ctggctaggc gttgacgaaa atacagtgct gaccaatcgg caaaaaatca gcaccaacgg
     1801 tgtgctgcaa tggtccacca tccgccgcct gaccgaagag gtgatgcagc ggatgacggt
     1861 caaggctgcc agtagcgaaa cacccatcgg cacgctttct ggtggcaatc agcaaaaagt
     1921 ggtgatcggt cgttgggtct atgccgccag ccagattttg ttgctcgacg agccaacgcg
     1981 cggcgtcgat atcgaagcca aacagcagat ttaccgtatt gtccgtgagc tggctgccga
     2041 aggaaaaagc gtggtgttta tctccagtga agtggaggaa ttgccgctgg tgtgtgaccg
     2101 catcctgtta ttacagcacg gcacgttctc gcaggagttt cactcgccgg tcaatgtgga
     2161 tgagctgatg tccgccattc tgtctgtgca ctgatgaatt ttaccgagga acctgaagat
     2221 gtatgcttcg tcattaccat tgccgcaggg caagagcgtc tcgctcaaac aatttgtcag
     2281 tcgccatatt aatgagatcg gtttgctggt ggtgattgcc attctttatc tggtcttctc
     2341 cctgaacgcg cctggcttta tctcattgaa taaccagatg aacgtgctgc gcgatgccgc
     2401 caccattggg attgccgcct gggcgatgac gctgattatt atctccggtg aaattgatgt
     2461 cagcgttggg ccgatggtgg cttttgtctc ggtgtgcctg gcatttttgc tgcaatttga
     2521 agttccgttg gcgattgctt gtctgctggt gttgctgtta ggcgcgctga tgggaacgct
     2581 cgccggggtg ctgcgcggcg tgtttaacgt accaagtttc gttgccacac tggggctgtg
     2641 gagcgctctg cgcggaatgg ggctgtttat gaccaacgca ctgccagtgc cgattgacga
     2701 aaacgaggtg ctggactggc tgggcggaca atttctcggt gtgccagtat ccgcgctgat
     2761 catgatggtg ttgtttgcgc tgtttgtgtt cattagccga aaaaccgcct tcgggcgctc
     2821 ggtttttgcc gttggcggta atgccacggc ggcgcagttg tgcggtatta acgttcgtcg
     2881 agtgcgcatt cttatcttta ccctttcggg attattagcg gcggtgaccg gcattttgtt
     2941 ggcggcacgc ctcggttccg gtaacgcagg tgccgcaaac ggtctggagt ttgacgtcat
     3001 cgccgcggtc gtggtcggcg gtacggcact ttccggcggt cgcggttccc tgttcggtac
     3061 attgcttggt gtgctggtga ttacgctaat cggtaacggt ctggtgctgc tcggtattaa
     3121 ctcctttttc cagcaggtgg tacgcggcgt catcatcgtg gtggcggtgc tggcgaatat
     3181 cttgctgacc cagcgaagca gtaaagcgaa acgctaaacc ttaacgcccc tggcgcgggt
     3241 gctgcaggga tgaaaattaa tcgtattgtt aatgagggat ttatgaaaac gatgctggct
     3301 gcttatttac taggaaattc aaccgtcgat ctgcgggaag ttgcggtgcc gacgccgggg
     3361 attaaccagg tactgatcaa aatgaaatcc tccgggattt gcggaagcga tgtccactat
     3421 atctatcatc aacaccgtgc cacggcggcg gcacccgata aaccgttata ccagggattt
     3481 atcaacggtc atgaaccgtg cgggcagatt gtggcgatgg ggcaaggctg ccgccatttt
     3541 aaagagggcg accgcgtgct ggtgtatcac atttctggct gtggtttttg cccgaactgc
     3601 cgccgcggct ttcctatctc ttgtactggc aaaggaaaag cggcttacgg ctggcagcgt
     3661 gacggcggtc atgccgaata tctgctggcg gaagaaaaag atctgatcct cctgccggat
     3721 gcgctgagct acgaagatgg tgcgtttatc agttgcggcg ttggcacggc ctatgaaggg
     3781 attttgcgcg gcgaagtttc cggcagcgat aacgtgctgg tggtcggtct ggggccggtc
     3841 ggcatgatgg cgatgatgct ggcgaaaggt cgcggtgcaa aacggatcat cggcgttgat
     3901 atgctgccgg aacgtctggc gatggcaaaa cagttagggg ggatggatca cggctattta
     3961 gcaaccaccg aaggtctgcc gcagataatt gctgaactta cccacggtgg cgcggatgtt
     4021 gcgctcgatt gttccggtaa tgccgcaggt cgcttgctgg cactgcaatc cactgctgac
     4081 tgggggcggg tggtttacat tggtgaaacc ggaaaagtgg aattcgaggt cagcgctgat
     4141 ctgatgcacc atcaacggcg gattatcggc tcctgggtga ccagtctgtt ccatatggaa
     4201 aaatgcgccc acgatttgac tgactggaag ctgtggccgc gtaacgccat tacccatcgc
     4261 ttctcgctag aacaggcagg tgatgcctat gtgctgatgg cgagcggcaa atgcgggaaa
     4321 gttgtgatta acttcccgga ttaaggagtt agcggatgac catctatacc ttatcccacg
     4381 ggtcgctaaa gctggacgtt tctgaccagg gcggcgtaat cgaaggcttc tggcgcgata
     4441 cgacgccgct cttacgtcct ggtaaaaaaa gcggtgtggc aaccgatgcc tcatgttttc
     4501 cgctggtgcc atttgcaaac cgggtgagcg gcaaccgttt tgtctggcag gggcgtgagt
     4561 atcaactgca accgaatgtt gagtgggatg cgcactatct acacggcgat ggctggctgg
     4621 gcgagtggca atgtgtctcg cacagcgatg atagtttgtg tctggtgtat gaacatcgca
     4681 gcggtgtcta tcactatcgg gtaagtcagg cgtttcattt aacggcggat acgctgacgg
     4741 tgacgctctc tgtcaccaat caaggggcag agacgctgcc atttggtacc ggctggcatc
     4801 cttattttcc gttgtcacca caaacgcgga ttcaggcgc
//
LOCUS       DADLTY010000245         4801 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.248, whole genome shotgun sequence.
ACCESSION   DADLTY010000245 DADLTY010000000
VERSION     DADLTY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.248"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..527
                     /locus_tag="JN334_003957"
     CDS             <1..527
                     /locus_tag="JN334_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAY7439916.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="JN334_003958"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="JN334_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY7439917.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3205)
                     /gene="gap"
                     /locus_tag="JN334_003959"
                     /pseudo
     CDS             complement(2205..3205)
                     /gene="gap"
                     /locus_tag="JN334_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3394..3924
                     /gene="cybB"
                     /locus_tag="JN334_003960"
     CDS             3394..3924
                     /gene="cybB"
                     /locus_tag="JN334_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HAY7439918.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4169..4342
                     /locus_tag="JN334_003961"
     CDS             4169..4342
                     /locus_tag="JN334_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439919.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="JN334_003962"
     CDS             complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="JN334_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HAY7439920.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4664..>4801)
                     /locus_tag="JN334_003963"
     CDS             complement(4664..>4801)
                     /locus_tag="JN334_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7439921.1"
                     /translation="EFSNISELKDAVTEYIEYYNSRRISLKLKGLTPNEYRNQTYMPR
                     V"
BASE COUNT         1226 a         1104 c         1198 g         1273 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 ttccgagaat ggacaccagc tcagtgaccg aacctgtttt caccggcacg cgttgcgcat
     2521 gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg
     2581 tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg
     2641 ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt
     2701 ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg
     2761 acgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattaacg ttataaacga
     2821 tggttttcat ttcaccggca ggagcggaaa tcaacacctt cttcgcacca gcatcaagat
     2881 gcgcctgcga tttatcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc
     2941 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
     3001 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg
     3061 aatcatgttt cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa
     3121 cgtctatgtt gcttttgact tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa
     3181 aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt
     3241 gatcctttcc aggcttagac caggattgtg ggatgggcaa tctccattct tacagtgaaa
     3301 cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg
     3361 ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa
     3421 atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt
     3481 cgtggtttct tcccacgtag cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc
     3541 atctcaattc tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg
     3601 ccgattatac ctaagccgaa accgatgatg acgggactgg cgcatttggg acatttggtg
     3661 atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc
     3721 aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg
     3781 gttgatagct taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg
     3841 ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc
     3901 atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac
     3961 ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat
     4021 gacaaataaa accataatcc ttgcccatac gtccatctgg cttattttta atcaattcac
     4081 ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgcac
     4141 ataagattcg ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg
     4201 aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg
     4261 gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc
     4321 gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag
     4381 tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc
     4441 agcaacctcc ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag
     4501 tgtgaatgcc agaatcgtaa tgcagataat gagcagacac accaccagag ggttgcgctt
     4561 catagccttt ctccttgccg gatggcgggt aagaggctaa caatctgaat tgcttggttc
     4621 attcgttggc ctcggtttgt actgacccca aaaagttgga cagttaaaca cgaggcatat
     4681 aggtctgatt ccgatattca tttggagtca gaccttttaa tttcaggcta attcttctgc
     4741 tgttgtagta ttcaatatat tccgtaacag catccttcag ttcgcttata ttactgaact
     4801 c
//
LOCUS       DADLTY010000246         4785 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.249, whole genome shotgun sequence.
ACCESSION   DADLTY010000246 DADLTY010000000
VERSION     DADLTY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.249"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..785)
                     /gene="tuf"
                     /locus_tag="JN334_003964"
     CDS             complement(<1..785)
                     /gene="tuf"
                     /locus_tag="JN334_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7439922.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEM"
     gene            complement(900..975)
                     /locus_tag="JN334_003965"
     tRNA            complement(900..975)
                     /locus_tag="JN334_003965"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(940..942),aa:Thr,seq:ggt)
     gene            complement(982..1056)
                     /locus_tag="JN334_003966"
     tRNA            complement(982..1056)
                     /locus_tag="JN334_003966"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1021..1023),aa:Gly,seq:tcc)
     gene            complement(1173..1257)
                     /locus_tag="JN334_003967"
     tRNA            complement(1173..1257)
                     /locus_tag="JN334_003967"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1221..1223),aa:Tyr,seq:gta)
     gene            complement(1266..1341)
                     /locus_tag="JN334_003968"
     tRNA            complement(1266..1341)
                     /locus_tag="JN334_003968"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1306..1308),aa:Thr,seq:tgt)
     gene            1703..2653
                     /gene="coaA"
                     /locus_tag="JN334_003969"
                     /gene_synonym="panK"
     CDS             1703..2653
                     /gene="coaA"
                     /locus_tag="JN334_003969"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAY7439923.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2682..3647)
                     /gene="birA"
                     /locus_tag="JN334_003970"
     CDS             complement(2682..3647)
                     /gene="birA"
                     /locus_tag="JN334_003970"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HAY7439924.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3644..4672)
                     /gene="murB"
                     /locus_tag="JN334_003971"
     CDS             complement(3644..4672)
                     /gene="murB"
                     /locus_tag="JN334_003971"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAY7439925.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1224 a         1211 c         1083 g         1267 t
ORIGIN      
        1 aacatttcaa cgccagtaca ggtagacttc tgagtctctt tgataccaac gatttcaact
       61 tcttcaccaa ctttgatgat accgcgttct acacgaccgg taacaacggt accacgaccg
      121 gagatggaga atacgtcttc gatcggcagc aggaacggct tgtcaatcgc acgctctggt
      181 tccggaatat aagaatccag gaagccagcc agttccagga ttttcgcttc ccactctgcg
      241 tcgccttcca gcgctttcag agcagaacca cgaacgatcg gagtgtcgtc gcccgggaag
      301 tcgtactgag acagaagttc acgaacttcc atttcaacca gttccagcag ctcttcgtca
      361 tcaaccatgt cgcatttgtt caggaacacg atgatgtacg gaacgcctac ctgacgaccc
      421 agcaggatgt gctcacgagt ctgcggcatc gggccgtcag tcgcagcaac taccaggatc
      481 gcgccgtcca tctgagcagc accggtgatc atgtttttaa catagtcggc gtgccccggg
      541 cagtctacgt gtgcgtagtg acgggtcggg gtgtcgtatt caacgtgaga agtgttgatg
      601 gtgataccac gagctttttc ttccggcgcg ttatcgatct ggtcgaatgc gcgagcagca
      661 ccgccgtagg ttttagccag tacggtagtg attgcagcgg tcagcgttgt tttaccatgg
      721 tcaacgtggc cgatagtacc gacgttaacg tgcggttttg tacgttcaaa cttttcttta
      781 gacatcgatt gtccctctaa gacacggata aatcggtgat atcaccacat caaccaggca
      841 acatgcccga cttgttgaat gcaataaaca gaaggaaaaa acagggagga gaaaagaagt
      901 ggtgctgata ggcagattcg aactgccgac ctcaccctta ccaagggtgc gctctaccaa
      961 ctgagctata tcagcacatc ttggagcggg cagcgggaat cgaacccgca tcatcagctt
     1021 ggaaggctga ggtaatagcc attatacgat gcccgcatcc tagaactcgg ctacctgatt
     1081 ttcattctgc actgaatatc gagagaagct ctctttattc gagccggtaa gtgaacttat
     1141 cgtctcgggc tacgccatcg cgtggccgaa attggtggtg ggggaaggat tcgaaccttc
     1201 gaagtctgtg acggcagatt tacagtctgc tccctttggc cgctcgggaa ccccaccgga
     1261 cttgatggtg ccgactaccg gaatcgaact ggtgacctac tgattacaag tcagttgctc
     1321 tacctactga gctaagtcgg catcaagtag cgcgcattct atggagacat gcgagttcat
     1381 gcaactaaaa aattgcataa tttgttttat tggtcacatt ttatgctaca cgatgaagaa
     1441 acagccgaac ggagggcaga ttagcacact ttttcaacat cattgtgctc aacaatgcgc
     1501 tcctgctaaa ccataattct ttttatcaga tggaatatct gtcacattgc ttttcaacga
     1561 tagcttcctg gcagagattt tttcttatta ttcctcccca tctggtgtta ccctcctgcc
     1621 cattaaccca ttcaacagaa ctgtgacgcg ccatggcaaa tatcgctttg ccgatagagc
     1681 tatgaccgcc agaaacatgc ttatgagtat aaaagagcaa acgttaatga cgccttacct
     1741 acagtttgac cgcaaccagt gggcagctct gcgtgattcc gtacctatga cgttatcgga
     1801 agatgagatc gcccgtctca aaggtattaa tgaagatctc tcgttagaag aagttgccga
     1861 gatctattta cctttgtcac gtttgctgaa cttctatata agctcgaatc tgcgccgtca
     1921 ggcagttctg gaacagtttc ttggtaccaa cgggcaacgc attccttaca ttatcagtat
     1981 tgctggcagt gtcgcggtgg ggaaaagtac aaccgcccgt gtattgcagg cgctattaag
     2041 ccgttggccg gaacatcgtc gtgttgaact gatcactaca gatggcttcc ttcaccctaa
     2101 tcaggttctg aaagaacgtg gtctgatgaa gaagaaaggc ttcccggaat cgtatgatat
     2161 gcatcgcctg gtgaagtttg tttccgatct caaatccggc gtgccaaacg ttacagcccc
     2221 tgtttactcg catcttattt atgatgtgat cccggatgga gataaaactg ttgttcagcc
     2281 tgatatttta attcttgaag ggttaaatgt cttacagagc gggatggatt atccacacga
     2341 tccacatcat gtatttgttt ccgattttgt cgatttttcg atatatgttg atgcaccgga
     2401 agacttactt cagacgtggt atatcaaccg ttttctgaaa ttccgcgaag gggcttttac
     2461 agacccggat tcctattttc ataactacgc gaaattaact aaagaagaag cgattaagac
     2521 tgccatgaca ttgtggaaag agatcaactg gctgaactta aagcaaaata ttctacctac
     2581 tcgtgagcgc gccagtttaa tcctgacgaa aagtgctaat catgtggtag aagaggtcag
     2641 actacgcaaa taatttgcag gggagcgaat actccccttt cttatttttc tgcactacgc
     2701 agggatattt caccgcccat ccagggtttt attattccat cctgctcaag taataaagcc
     2761 ccctgtttgt ctattccgcg tgaaatgcca aatatttctt tatcaccaat gataagtttc
     2821 actgggcgat taataaaatt atccagcttt tcccagcgcg acagataagg tgccaatcct
     2881 tcttgttcga agagttccaa cgcagcacgt aattcacgta ttagcatggc cgccaacgta
     2941 ttacgatcaa gattgatccc cgcttcctgc agcgtgatcc acccctgatt aacgacactc
     3001 tcttcaacac ggcgcattgc catgttgatc ccggctccaa tgactatttg cgccgcatcg
     3061 ccagttttgc cagtcagctc cacaagaatg cctgccagct tgcgatcctg cagatagagg
     3121 tcattaggcc atttgacacg aactttatct gctcccagct ttcgtaatac ttccgccatc
     3181 acgataccga taaccagact taaaccaatc gccgccgccg ggccttgttc cagacgccag
     3241 aacatcgaca aatataagtt tgcgccaaaa ggcgaaaacc atttccgccc ccggcgacca
     3301 cggccagcct gctggtattc tgcaacacag gcatcgcccg atttaagctc tccgatacga
     3361 tcaagaagga actgattcgt ggagtcaata actggcagca cggctacact accgccatcc
     3421 agctgaccca atatctgttc agcattaagt aactggatgg gctcaggcag gctgtatcct
     3481 ttacccggaa cggtaaagac atcaacgccc cagtcacgca gtgtctgaat gtgtttatta
     3541 atagccgccc ggctcattcc cagcgtttca cccaactgct cgccagagtg aaattcaccg
     3601 ttcgctaaca gggcaatcaa tttcagtggc acggtgttat ccttcatgaa attgtctcca
     3661 ctgcgctcac ttcacctgat gcaccaataa agcggacttc aggctcaagc cagacattaa
     3721 atttttcacc cactttttgt cttacatggt gtgccagttg caccacatct tcgctttttg
     3781 cattgtcttc attaatgaga actaacgcct gttgacggtg caccgcagcc ccacccattt
     3841 gcatcccttt tagctggcac tgatcaataa gccaacctgc tgccagtttt actgaaccac
     3901 ccgcctgggg gtaatttggt gctgttggaa attgcgccag taatgcttta gccgtttcgg
     3961 cagatacaac agggtttttg aagaaactac cggcattgcc attcactttt ggatcaggga
     4021 gtttggtggt gcgcatatga cacaccgcat taaatacttg ctgtggcgtt actgttgtag
     4081 gatccagacg agttaagtca ccatacgtta gtacaggttg ccactctttt ggcagacgca
     4141 gacctacggc tacaatggcg aagcgatcct ggtattcatg tttaaaaata ctgtcgcgat
     4201 agccaaaacg gcactctttg gcagttaagc gcacttgctt gcctgtcgcc agttcaacac
     4261 aatcaacata agcgcaaact cgctgtaatt ctacgccata agcaccaata ttctggatag
     4321 gtgatgagcc gacacaacca ggaattaatg ccagattttc cagaccaggc ataccttcct
     4381 gcaaagtgta ttttaccagg cgatgccagt tttctccggc tcctacatgt aaataccacg
     4441 catcaggttc atcatgaatt tcgatacctt tgatccggtt gatgatcacc gtgccgcgat
     4501 agtcttccag aaaaagtaca ttacttcctt cacccagaat aagaacgggt tgtccttctg
     4561 cggttgcatg ctgccaggca ttgagtaatt gttgttcgtc ttcggcacat acaatgtgct
     4621 gagcattatg atcaatgcca aatgtgttcc agggttttaa ggagtggttc atagctgctt
     4681 tcctgatgca aaaacgaggc tagtttaccg tatctgtggg gggatggctt gtagatatga
     4741 cgacaggaag agtttgtaga aacgcaaaaa ggcccgactg ggctt
//
LOCUS       DADLTY010000247         4783 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.250, whole genome shotgun sequence.
ACCESSION   DADLTY010000247 DADLTY010000000
VERSION     DADLTY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.250"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(267..1124)
                     /gene="sitD"
                     /locus_tag="JN334_003972"
     CDS             complement(267..1124)
                     /gene="sitD"
                     /locus_tag="JN334_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HAY7439926.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="JN334_003973"
     CDS             complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="JN334_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HAY7439927.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="JN334_003974"
     CDS             complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="JN334_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HAY7439928.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2802..3692)
                     /gene="sitA"
                     /locus_tag="JN334_003975"
     CDS             complement(2802..3692)
                     /gene="sitA"
                     /locus_tag="JN334_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAY7439929.1"
                     /translation="MLLGCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
     gene            complement(4148..>4783)
                     /locus_tag="JN334_003976"
     CDS             complement(4148..>4783)
                     /locus_tag="JN334_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7439930.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1268 a         1169 c         1129 g         1217 t
ORIGIN      
        1 gagctgaagg aagagctgaa tagtctgaag gatttgaatt cggttaggaa ttttatcgtg
       61 gaataacaga agctgcggca cgtcgtatgc aggaacgtgc cgcggttggc tggtaaactt
      121 tcgatagtgc gagtattgaa tgatttctag gcgttatcga ttttacgtat tttttgcatg
      181 atagaattcg tacctcctcc caacgacctt ccatggcttt ccggacttct gtaggctggc
      241 ggacgatgct gtcctcggat gttgtgctaa ttgggtgata acagatcctg tgtatgggcg
      301 tttccctttg tgcgtgcgtt gatgctggta acggtaaacg tcatgatgaa cacgaccgcg
      361 aataagacga caatcgtcgg agctggtgcg ctgtcgataa aaaaggacag ccagacacca
      421 gtcattgaca ctattaccga tatgccagtc gccagtagga gcgcaatatg aaagcgttgt
      481 gtcaggagta ctgcaatggc gccaggcgca atgagcagag aaatagagag aatgatacct
      541 actgctttca gcgtcgccac aatggtcagg gaaaccatgc acagcagccc ataatgcagc
      601 catctcgtat gcaaaccgct tacctgcgcc tgttgataat caaagctgaa cagcaaaaaa
      661 tcccgccatt ttacgctaat aacaagtgta accagcccgg caataatcac cgtctggatt
      721 atgtcgccga tggttatccc gagcatgtcg ccgaaaagaa tatggtcaag atgtacgtct
      781 ggtttaattg caatatacaa gatcagacct gcggcaaaca tgccagaaaa aacaatcccc
      841 ataactgtat cttgcttgat tcggctgttg tctttcaggt atcccgtcgc caccgcgcag
      901 aatactccgg cgacgaatgc ccctgtagcc agaggtaacc ccagaatcca ggtaagcacg
      961 atgccaggaa aaaccgcatg actcatcgcg tcacccatca gtgcccaacc tttcaggacc
     1021 agaaagaccg acagcagcgc acagggtatg gaaactacca gtgaaatcag cagtgcgtga
     1081 ctcataaagg tgaactgcag tggctccagt aataatgcca tcatgagcga tcctccagcg
     1141 ctttatgcgc acgccgacga tttgccaaaa gtccatgggt gggcgcaaat acaaatgcta
     1201 aaagaaacag cagtgtctgc gccaccacga tgattccacc tgtcgcgccg tccagaaagt
     1261 aactgaccca cgcgccgagg aaactggtta cactgccaat agttacagca attatgagca
     1321 ggcggggaaa acgatcggtt aacaaccatg ctgttgcacc gggagtcacc accagacaga
     1381 tcaccagaaa ggcaccaacc gtttgcagcg ccgcaacggt cgaaaccgac agcagggtga
     1441 agaagaggat ttttaatctg cccggatgta atccaatagc acgggcgtga ttctcgtcga
     1501 aaaaggtcac cataagatct ttccatttga aaaaaagcac gatgatcgag agaatgccaa
     1561 tgatagtgag ttgcaggata tccgccggat cgattgccag aatattgccc aggacgatgg
     1621 tctgaatgtt cacggatgtc gggttcaatg acaccataaa cagccccagg ccaaaaaagg
     1681 acgagaagat caggccgatg atggcatctt cttttaagcg ggtgcgttgg ttaagaaaca
     1741 gcatgctgcc cgctgccagt ccgccggaaa agaaggctcc cagagaaaac ggaagcccca
     1801 gcatatatgc ccccgcaaca cccgggacaa tagagtgcga cagcgcatcg ccaatcagcg
     1861 accagccttt tagcatcaga tagcatgaca ggaaagcgca gagaccgccg accattgccg
     1921 agacccacat cgcattgagc atatattcat agctgaaggg ttccagtagc acgttcatct
     1981 tgcttccctc tgcaccgctg acggtcgatg tgccacaaaa gggcgttcat cgtcagtaat
     2041 aatgctttct tccgagccgt taagtgtgac atggcgcagt acgccgctaa aagccagctc
     2101 caggttctcg gctgtaaaag tggtgtctgt cggcccgctt gccaggacgg tgcctttgac
     2161 catgaccgta taatcgcaaa atgtcgtgac tgagccgaga ttgtgcgttg agacaagcat
     2221 cgttttgcct tccgcgcgta actccctcaa caggctgatg attttcgctt cggttttcac
     2281 atcaacgccg gtaaaaggtt catccagcag gatcacatca ccctgctgtg caattgctct
     2341 cgccagaaag acgcgtttct tttgtccacc ggaaagctcg ccgatttgac gatggcgaaa
     2401 atccaccata tcgacacgtt ccagcgcatc ggtgacaatc tgacgatccc tctttttggc
     2461 tatacgcaac atacccatat gcccatagcg gcccatcatc accacatctt ccaccagtac
     2521 aggaaatgac cagtcaacct cttctgactg agggacgtag gcaaccaggt ttttctgtag
     2581 cgcctgtcgc gtgggaatac ccagaacaga tatctttccg ctggtcagac gcacaaatcc
     2641 cataatcgct ttaaacaacg tcgattttcc ggaaccgttt accccaacca gagcggcaat
     2701 agatccactt ggtactgtga aggatgcatc gcgcagtgct gtgtgcccgt tacgccaggt
     2761 gacagtaaca tcattaacga caatgcctgc agattgcatc attatttctc ccttttcccg
     2821 gctttaattc cctgtaccag cgtactggta gtaaccttaa gaaggtcgat atacgtaggt
     2881 accgggccgt tttctgtgct cagggaatcg acatagagca caccgccgta gtgtgcgccg
     2941 gtttcacgcg caacctgacg cgctggttta tcggaaatcg tactctcgct aaagactgcc
     3001 gggatatgat ttttcttaac tatatcaaca accttacgta cctgctgcgg tgttccttgt
     3061 tgatcggcat taatcggcca cagataaagc tcttttagcc ccaaatcgcg tgcgagataa
     3121 gaaaaagccc cttcactggt gaccatccat cgctgattct cagggagttc cgtaatctgc
     3181 ttacgcaggg gggcaagggt ttgggtaatc ttggctttat aagtatcggc attacgttgg
     3241 taggtttgtg catttgccgg atcgtatttt atcaacgcat cacgaatatt atcgacgtaa
     3301 atcagagcat tatctggcga catccatgca tggggattag gtttgccctc atagggtcct
     3361 tcggtgatcc ctactggcgt cacacccgaa gagacaatta cttctggaac cccattgaga
     3421 tgctggtaaa agcgttggaa ccacaattcc agattcatac cattggcgag aatcagttgt
     3481 gccccctgcg cacgtttaat atcgccaggg gtaggctgat actcatgaat ttctgcacca
     3541 ggcttggtta tggatgagac ttctgcagca tctccagcca cgtttttggc catatccgcg
     3601 atgatggtaa atgttgtaat gaccttgaat ttttcagttg cacttgcctg aaatgcgatc
     3661 gagcaggtga gggcgaggca ccccaagagc atggttactt tttttattga gtgcataatt
     3721 tagtacctat agtgagcaaa attagaacaa acatagcaac ggctatgttt tatagcataa
     3781 cctatacaaa atgaaaataa ttatcattaa cagctttgag gcctaaaaca gggattaggt
     3841 tgtttcctgc tggcgacatg atattgagga aaaaagtaga gcagctatta ggctttgaaa
     3901 tgactttatg aggaaaattg ttgatggcaa aaatggggca aaaaagagtg ggttatcgta
     3961 gcttattgtt gtcgctgata atgtttaaca tattgaaaaa taaataaaat acttatgagt
     4021 cagatagttg tgatttttgc ccttacttgt tcaggttgta ttgttctttc tttgtaattt
     4081 gttgattttc ttgcattatt tcagttctct ggtactaaat ggggcaaatt gggggcaaac
     4141 tttgcaacta cgataaccgc gcattcaaca tggctatctg ttcgtcgttc atgtcatcaa
     4201 tccacatacc gtaaatttca tacaccattt gcgcagtttc atgccccatc tggctggcta
     4261 taaatgccgg gttcgctcct gccgtcaaca gccagcaggc aaaagtatgt cgcgtatggt
     4321 acggattacg gcggcgaata ccagcacgtt ttactgctgc attccatctt gtacccaaac
     4381 tgcttaccga gtaataaggt ttctgttttt cgttacacac cctgggcatg aaaacaaaat
     4441 gcagtttttg tttttcagtt ctgccgtact cccgatgata aaaagtgatt tcgcttttgc
     4501 gatgatgccc ggtcagtttg tattgctcct tcagtgcttc aagagccggc tgcagtaatg
     4561 ctaccgtccg gatcccggca tttgtttttg ggggaccgaa catatcaagt atcgtcaggt
     4621 ttcttctgac attcacaatt cccttctcga aatccacatc ctcccacgcc agagctgcca
     4681 gttccccgtg acgaagcccg gagtaaacgg caaatttcca caagttttgg ctctgtcctt
     4741 tttcactttc cattaatgca ttgaattctg ttttagataa cgg
//
LOCUS       DADLTY010000248         4726 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.251, whole genome shotgun sequence.
ACCESSION   DADLTY010000248 DADLTY010000000
VERSION     DADLTY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.251"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            64..320
                     /locus_tag="JN334_003977"
                     /pseudo
     CDS             64..320
                     /locus_tag="JN334_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421719.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            463..684
                     /locus_tag="JN334_003978"
                     /pseudo
     CDS             463..684
                     /locus_tag="JN334_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(705..767)
                     /locus_tag="JN334_003979"
                     /pseudo
     CDS             complement(705..767)
                     /locus_tag="JN334_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1217..2629
                     /gene="ospC1"
                     /locus_tag="JN334_003980"
     CDS             1217..2629
                     /gene="ospC1"
                     /locus_tag="JN334_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HAY7439931.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2733..2882)
                     /locus_tag="JN334_003981"
                     /pseudo
     CDS             complement(2733..2882)
                     /locus_tag="JN334_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2958..4655
                     /gene="ospD3"
                     /locus_tag="JN334_003982"
     CDS             2958..4655
                     /gene="ospD3"
                     /locus_tag="JN334_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HAY7439932.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1642 a          849 c          884 g         1351 t
ORIGIN      
        1 ttgagaggcg ggtttacgtt tacgggattt agctgaacgg gccatataac cacctgaaag
       61 acaatgacat tgcctgtttt tataacggta attgcagacc atgacaaacc gcagccgtca
      121 ggctgcctac tcgagtcaca aggctctttg tgtccgatat gccggcagcg cattacccat
      181 gaaactggct ggaacgtaca ccataaggtg aagaaagtta tgggggcggt gaagaattat
      241 ctaaccttgt cttgttacat ccaaattgcc atcgacagtt acacagttgt gaagccggct
      301 ctctcaaaaa gggcttataa aggcttgagc ggtgtaccgg gaaaccggta tgcaccgttc
      361 ttaggggagg gcagtccagc aatgaactgt ccttacccga caaacatcca aaacgaacgg
      421 aacgtgctgg aatccgcata taacccgttg taaagtgagg aaatggacac ctcacttgct
      481 catgagaacg cccgcctgcg ggcactgttg cagacgcaac aggacactat ccaccagatg
      541 gctgaataca accgcctgct ctcacagcgg atggcggctt atgcttccga aatcaaccga
      601 ctgaaggcgc tggttgcgaa actgcagcgt atgcagttcg gtaaaagctc agaaaaactt
      661 cgcgcaaaaa ccgaacggca gattccattt tcacgggcga tctacgccac acaggctctg
      721 ggttgcagtg actgctccat taaggcgatt ttgaactcat aagggaagta acaaaaaaac
      781 tgcaaaagta caataattat atatggccaa actaacctct attaaaaaac atcagttttt
      841 cacacttagt tgagcttcat gaaaaaatca tcgaggattt cattgcagaa tacacattcc
      901 actaactgga agcactccat aagaggaatc agagaaagta tcgttttttt atagtaaaat
      961 tcattgctgt tcaataaaat tgatatagtt attaaattat gaaaataatt tcacaaaaaa
     1021 gattaacttg agttcttaag catcggattt tagatatttt gaaatatctg gcatcacata
     1081 acatcaaaag tatataatac tcttatctaa ttacatgcag tatattgtat atatactcaa
     1141 aaattgtaat tcataccctg ctatatcttg tagggaaaga tattaattaa aactgttttc
     1201 atataaggtt cattttatga atatatcaga aacactgaac tcagcaaata cccaatgcaa
     1261 tatagattct atggataaca gattacatac attgtttcca aaagtgacat cagtgcgaaa
     1321 cgctgcacaa caaactatgc cagatgaaaa aaatttaaaa gatagtgcaa atattattaa
     1381 aagtttcttt aggaaaacta tagcagcaca gagttatagt agaatgttct ctcaaggctc
     1441 taactttaaa tctttaaata tagcaattga tgcaccatca gacgctaaag cctcatttaa
     1501 ggctattgag caccttgaca gattatcgaa gcattatata tctgaaataa gggaaaaact
     1561 tcatcctctt tctgcagagg aactcaattt gctttcgcta attattaatt ctgatttaat
     1621 cttcagacat caaagtaatt ctgatttgtc tgataaaatt ttaaacatta agtcattcaa
     1681 taaaattcag tctgaaggaa tatgcacaaa acgaaacaca tacgctgatg atataaaaaa
     1741 aatagctaat catgactttg tgttttttgg cgttgaaatc tctaaccatc agaaaaaaca
     1801 ccccctgaat acaaaacatc acactgttga ttttggtgca aatgcgtata tcattgatca
     1861 tgactctcca tatggatata tgacattaac cgatcacttt gataatgcta ttccacctgt
     1921 tttttaccat gagcaccaat catttttaga taaattttca gaggttaata aagaagttag
     1981 tcgatacgta catggaagta aaggaattat agatgtacca atattcaata ctaaagatat
     2041 gaagttaggg ctcggattat acctgattga ctttattaga aaaagtgaag accaaagctt
     2101 caaggagttt tgctatggaa aaaatcttgc ccctgtggat ctggatagaa tcataaactt
     2161 tgtttttcag ccagagtacc atatacctag gatggtaagt acagaaaact tcaaaaaagt
     2221 taagattaga gaaatatcct tagaggaggc tgttacagca tctaattacg aagaaattaa
     2281 caagcaggtc actaacaaaa aaattgctct ccaggctctt tttctttcga ttactaatca
     2341 aaaagaggat gtcgccttat atatattatc taattttgag ataactagac aagatgttat
     2401 ttccataaag catgagttgt atgatattga gtatctactt agcgctcata attcaagctg
     2461 taaagtactt gagtatttta tcaataaggg attggttgat gtaaacacaa agttcaaaaa
     2521 aactaatagt ggggattgta tgttggataa cgcaataaaa tatgagaatg cagaaatgat
     2581 aaaactatta ttgaaatatg gtgcaacatc tgacaataaa tatatttaat caaaattgaa
     2641 tatcgtttag caaaaggcag tatttgcaca aaatatcgat atattgttta ttgtcagtat
     2701 ggctcaatgt gataatagtt ggaaagtttg atgggtttcg ccccgttgta gcggtagtcg
     2761 aactggaagg tcttcaggca ctgcttacag cgatagtgca gcccccctga actggaatgg
     2821 ccgttccgga tacccccctg agtttcagag taacggtgac aaaccacatc aatctttgcc
     2881 atcaatcatc caaagggcaa agagtacaac aacactaagt ctgcgtcaca acccatcaat
     2941 gaaaggaata tatacatatg ccatcagtaa atttaatccc atcaaggaaa atatgtttgc
     3001 aaaatatgat aaataaagac aacgtctctg ttgagacaat ccagtctcta ttgcactcaa
     3061 aacaattgcc atatttttct gacaagagga gttttttatt aaatctaaat tgccaagtta
     3121 ccgatcactc tggaagactt attgtctgtc gacatttagc ttcctactgg atagcacagt
     3181 ttaacaaaag tagtggtcac gtggattatc atcactttgc ttttccggat gaaattaaaa
     3241 attatgtttc agtgagtgaa gaagaaaagg ctattaatgt gcctgctatt atttattttg
     3301 ttgaaaacgg ttcatgggga gatattattt tttatatttt caatgaaatg atttttcatt
     3361 ccgaaaaaag cagagcacta gaaataagta catcaaatca caatatggca ttaggcttga
     3421 agattaaaga aactaaaaat gggggggatt ttgtcattca gctttatgat cccaaccata
     3481 cagcaactca tttacgagca gagtttaaca aatttaactt agctaaaata aaaaaactga
     3541 ctgtagataa ttttcttgat gaaaaacatc agaaatgtta tggtcttata tccgacggta
     3601 tgtctatatt tgtggacaga catactccaa caagcatgtc ctccataatc agatggcctg
     3661 ataatttact tcaccccaaa gttatttatc acgcgatgcg tatgggattg actgagctaa
     3721 tccaaaaagt aacaagagtc gtacaactat ctgacctttc agacaatacg ttagaattac
     3781 ttttggcagc caaaaatgac gatggtttgt caggattgct tttagcttta caaaatgggc
     3841 attcagatac aatcttagca tacggagaac tcttggaaac ttctggactt aaccttgata
     3901 aaacggtaga actactaact gcggaaggaa tgggaggacg aatatcgggt ttatcccaag
     3961 cacttcaaaa tgggcatgca gaaactatca aaacatacgg aaggcttctc aagaagagag
     4021 caataaatat cgaatacaat aagctgaaaa atttgctgac cgcttattat tatgatgaag
     4081 tacacagaca gatacccgga ctaatgtttg ctcttcaaaa tggacatgca gatgctatac
     4141 gcgcatacgg tgagctcatt cttagccccc ctctcctcaa ctcagaggat attgtaaatt
     4201 tgctggcctc aaggagatat gacaatgttc ccggacttct gttagcattg aataatggac
     4261 aggctgatgc aatcttagct tatggtgata tcttgaatga ggcaaaactt aacttggata
     4321 aaaaagcaga gctgttagaa gcgaaagatt ctaatggttt atctggattg tttgtagcct
     4381 tgcataatgg atgtgtagaa acaattattg cttatgggaa aatacttcac actgcagacc
     4441 ttactccaca tcaggcatca aaattactgg cagcagaagg cccaaatggg gtatctggat
     4501 taattatagc ttttcaaaat aggaattttg aggcaataaa aacttatatg gaaataataa
     4561 aaaatgaaaa tattacacct gaagaaatag cagaacactt ggacaaaaaa aatggaagtg
     4621 attttctaga aattatgaag aatataaaaa gctgaatatt atctcgtggt gatacactga
     4681 atttgaggta accggctcat ttaaaccgtc tggtctgtta cctccg
//
LOCUS       DADLTY010000249         4676 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.252, whole genome shotgun sequence.
ACCESSION   DADLTY010000249 DADLTY010000000
VERSION     DADLTY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.252"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..1075
                     /locus_tag="JN334_003983"
     CDS             <1..1075
                     /locus_tag="JN334_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7439933.1"
                     /translation="AEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTA
                     STDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPV
                     NVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVA
                     GQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYV
                     SYVHDLPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENI
                     SDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAIT
                     LHDAYKASDERLYVNKQNKNSRS"
     gene            1118..1621
                     /gene="yeaK"
                     /locus_tag="JN334_003984"
     CDS             1118..1621
                     /gene="yeaK"
                     /locus_tag="JN334_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HAY7439934.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1622..1726)
                     /locus_tag="JN334_003985"
     CDS             complement(1622..1726)
                     /locus_tag="JN334_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439935.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1896..2342
                     /locus_tag="JN334_003986"
     CDS             1896..2342
                     /locus_tag="JN334_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAY7439936.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2299..2938)
                     /locus_tag="JN334_003987"
                     /pseudo
     CDS             complement(2299..2938)
                     /locus_tag="JN334_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515112.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            3214..4394
                     /gene="nimT"
                     /locus_tag="JN334_003988"
                     /pseudo
     CDS             3214..4394
                     /gene="nimT"
                     /locus_tag="JN334_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4449..>4676
                     /locus_tag="JN334_003989"
     CDS             4449..>4676
                     /locus_tag="JN334_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAY7439937.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHE"
BASE COUNT         1105 a         1147 c         1166 g         1258 t
ORIGIN      
        1 tgctgagcag tcggaagtgt cgccagaaca gcggcgcgcc atctgcgagg cttttgagtc
       61 tgccaataat acccatggct taaacctgac tgcccataaa tacccgggct tacgcggcac
      121 actacaaacc gcatccactg actgcgacac aattgtggaa gctgcagcac tattacccgc
      181 ttttgatcag gcagtggaag gcaaccgcca ccaggatgat tacggttcag gtcttgggat
      241 ggccgaagag aaatttcact attatctcga tctcaatgac cgctatgtct atttttatga
      301 gccggttaat gttgaatact ttgcgatgaa taactggtcc ttcctgcagt caggaagtat
      361 tggcatcgat cgcaaagata ttgaaaaggt atttaccggg cgtaccgtat tgtcgagcat
      421 ttaccaggat cagcgtacta aacagaacgt gatgagtttg ctgacgccgg tatatgtcgc
      481 agggcagcta aaagggattg tgctgctgga tattaacaaa aacaatttgc ggaatatctt
      541 ttacactcat gaccgccctc tcctctggcg ttttctcaat gtcacgctaa ccgataccga
      601 ttcggggcgc gacattatca tcaaccagag cgaagataat ctgttccagt atgtcagtta
      661 cgtccatgac ttacctggcg gcattcgtgt ctcgttatcc attgatattc tttactttat
      721 cacgtcttcg aggaaaagcg ttctgttctg gattttgacg gcgttaattt tgctgaatat
      781 ggtccggatg cacttccgtt tataccaaaa tgtgtcgcga gaaaatatta gtgatgcgat
      841 gactggactg tataatcgca aaattttaac ccctgaactg gagcagcggt tgcagaaact
      901 ggtgcaatcc ggttcttcgg tgatgtttat tgcaattgac atggacaagt taaagcaaat
      961 aaatgacacc ctcggtcatc aggaggggga tttagcgatt acgttacatg atgcgtataa
     1021 agcttccgat gagcgtttat atgtcaataa gcagaacaaa aacagccgtt catgataacc
     1081 ttctgtggtt gtttgcttgt aatctcagga gcgtgaaatg actgaaatgg ctaaaggaag
     1141 cgtgacgcat cagcagttaa tcgctttatt atcacaagaa ggtgctaact tccgcgtcgt
     1201 tacccatgaa gcggtaggta aatgtgaagc ggtgtcagaa attcgtggta ccgctctagg
     1261 ccagggtgct aaggcactgg tatgtaaagt caaaggcaat ggcgtaaatc aacatgttct
     1321 ggcgatcctc gcagccgatc aacaggccga tctgagccaa ctcgccagcc atataggcgg
     1381 attacgcgct tcgttggcca gcccggcgga agttgatgaa ctcacaggct gtgtcttcgg
     1441 cgcgatcccc cctttcagct tccatccaaa actcaaactg gttgccgacc ctctactctt
     1501 cgaacgattt gatgaaatcg ccttcaatgc agggatgctg gataaatccg ttattttgaa
     1561 aaccgcggat tatttgcgca ttgcgcaacc agaactggtc aatttccgcc gcactgcgta
     1621 gctagcctat ccgttcaata agcagaacgg acaaaaccag cacaaccgca atagcaaaaa
     1681 acgatgatgt gataatcagt gtctcgacga acatttgatc gttcatagca tgcccccggg
     1741 agatagctgt tttcacgtta ttgttagcgt gcacaaatgg cagtttgatg acagttcgcc
     1801 tgatttttta tgcaaaaaac gtaaagattt tctacttcct tcctgcagca agcataaagt
     1861 aagcaggctt attatttttt ggcaaggaaa ccacgatgtt tgatgtcact ttgctgatcc
     1921 tgctcgggtt agctgcgctg ggctttatca gtcataacac cactgtcgcc gtttcaattc
     1981 tggtgttgat cattgtccgc gtcacaccgt taagcacctt tttcccgtgg attgaaaaac
     2041 aggggctaag tatcgggata atcattctga cgattggcgt tatggccccc atcgccagcg
     2101 ggacgctacc gccctcaacc ttgattcact cgtttctgaa ctggaaatcg ctggtggcaa
     2161 ttgcagtagg cgttattgtc tcctggctgg gtgggcgcgg cgtgacgtta atgggcagcc
     2221 agccgcaact ggttgctggg ctgctggtgg gcactgtttt aggcgtagcg ctgtttcgcg
     2281 gcgtaccggt tggcccgctt attgccgcgg gtctggtttc gctgattgtg gggaaacagt
     2341 agttaatccc gcaatatatc ggcctggcgt ttgccccaag cctttcttaa acatagtaat
     2401 aaaggccgtt gttgaatcat accccagcgt atgagcaact ttctgtacag tatcgccttt
     2461 cactaatccc tgaagcgcca taatcagctg taactgctga cgccactggc gaaaactcaa
     2521 ccccgtctct ttcactatca ggcgggcaag attgcgttca ctcatcgcaa aaaagccagc
     2581 ccattgcccc aatgcccccc actcgacggg ccctttcgcc atcatctcca ccatcgtgcg
     2641 gattttagga tgagaagaaa cgggcaggtg cagctgttgt tgtggctgct gagggagttc
     2701 atcaaacagg acctgaatta atctgcgggt catcggctca gcacgctgag tcgtcgtcct
     2761 gtttgccagg gttaaaatca attcccggca taagggcgat attttcaatg tgcaacatgg
     2821 tcggcattgt cactgcgctg ggttcgataa acagaaaaca gagttcagcg ttagctgtca
     2881 cttgattgct atgcttcacc ccgcccggta tccaaaccgc atattgtggc ggcaccatcc
     2941 acaaagcatt ttccaccgta caggtaattg caccatgtaa tgccagtatc agctgtcctt
     3001 tgcggtgctg atgtacagga ctggttaact catccgttcc ggcgtgaata caaaatgcga
     3061 ccgcggcttc atggtgtcgg tgggttcata gccattgaga ttcaacctgt gcatcatttt
     3121 gtccgaactt agcgataatt tgtcatttta gcttgattca acataacaat aaaaacggta
     3181 aggtacagcc tcgtttgtaa caatgagaag catatgacct gttcaacttc attaagcggc
     3241 aaaaacagga ttgtccttat cgctggcatt ctgatgattg ccacaacatt acgcgtcacc
     3301 tttaccggcg cagcgccgtt actggatacg attcgttccg cttactcgct gacgacagcg
     3361 caaaccggct tattaaccac cctgccatta ttggcctttg cgctaatctc acctctggct
     3421 gccccggtgg cgcgacgttt tggtatggaa cgtagcctgt ttgccgcgtt acttttgatc
     3481 tgtgcaggta tcgcaattcg ttctctccct tcgccttact tattatttgg cggtacagcg
     3541 gtcattggcg gtgggattgc attaggcaat gtcttactgc caggattaat taaacgcgat
     3601 ttccctcatt ccgtcgccag acttaccggc gcatattccc tgacaatggg agctgcagcg
     3661 gcactgggat cggctatggt cgtgccgctg gctttgaacg gttttggctg gcaaggcgcg
     3721 ttgttcatgc tgatgtgttt tcctctgctg gctctttttt tatggctgcc acagtggcga
     3781 agtcagcaac atgcaaattt gagtacctca cgcgccttac atactcgggg tatctggcgt
     3841 tcgccacttg cctggcaggt aacattgttt cttgggatca actcactggt ctattacgtg
     3901 attattggct ggcttccggc gatcctcatc agtcacggct atagcgaagc acaggcgggt
     3961 tcactgcatg gtttgctgca actagccaca gcagctcccg gtttgctgat cgcacttttc
     4021 ttacatcatg tgaaagacca gcgtggtatt gcagcgttcg ttgccttgat gtgcgcagtg
     4081 ggcgcggttg ggctctgctt tatgccagcg cacgcgatca cctggactct gcttttcggt
     4141 tttggttccg gcgcaacaat gatactgggg ttgacgttca ttggtcttcg gcaagttctg
     4201 cgcatcaggc ggcggcactc tcggggatgg cacaatccgt cgggtatttg ctggcagcct
     4261 gtgggccgcc gctaatgggt aaaatacacg atgctaacgg taactggtcc gtaccactga
     4321 tgggtgttgc aatactttca ctactgatgg cgattttcgg actttgcgcc gggagagaca
     4381 aagaaattcg ctaatatccg gtgctatagt gacgtgacaa atcttgcgtg aaagggagaa
     4441 caaacacgat gaatattcag tgcaaacgcg tttatgatcc ggctgaacag agcgatggtt
     4501 atcgcgtact ggtcgaccgc ctctggccgc gcggtatcaa aaaaaccgat ttagcccttg
     4561 atgagtggga taaagaaatc acgccgtcaa cggaactgcg caaagccttt cacggcgaag
     4621 tcgtcgattt tgcaaccttt cgcgagcaat atcgcgcaga actggcgcaa cacgag
//
LOCUS       DADLTY010000250         4630 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.254, whole genome shotgun sequence.
ACCESSION   DADLTY010000250 DADLTY010000000
VERSION     DADLTY010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.254"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            119..4237
                     /gene="pic"
                     /locus_tag="JN334_003990"
     CDS             119..4237
                     /gene="pic"
                     /locus_tag="JN334_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HAY7439938.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4388..>4630)
                     /locus_tag="JN334_003991"
     CDS             complement(4388..>4630)
                     /locus_tag="JN334_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAY7439939.1"
                     /translation="AYPTDIRKVIYTTNAIESLNSVIRHAIKKRKVFPTDDSVKKVVW
                     LAIQSASQKWTMPLKDWRMAMSRFIIEFGDRLDGHF"
BASE COUNT         1240 a         1050 c         1226 g         1114 t
ORIGIN      
        1 acaaaaacac attaatgcag taactatatt ttcctttctg gtgataacgt cgggttatca
       61 ttagcttctt cagctatttt acttttatat cccttgtaaa catcatggag aatccatagt
      121 gaataaagtt tattctctta aatattgccc cgtcaccggg gggcttattg ctgtctctga
      181 acttgcccgc agggtaataa aaaagacatg ccgaagatta acgcatattc ttctggctgg
      241 cattccagca atctgtctgt gttactctca gatatcccag gcgggtattg tccgttccga
      301 tattgcctat cagatttatc gtgatttcgc cgaaaacaaa gggctttttg tacctggtgc
      361 caatgatatt ccggtatatg ataaggacgg aaaacttgtg ggaagactgg gtaaagcccc
      421 aatggccgat ttcagcagtg tgagctcaaa tggcgttgct acgcttgtat cgcctcagta
      481 tatcgtcagc gtaaagcata acggaggata tcggagtgtg agctttggta atgggaaaaa
      541 tacatattcc cttgttgacc gtaataacca cccttctatt gacttccatg ctccacgtct
      601 gaataaactg gttacagaag ttattccctc agcggtaaca tcagaaggaa ccaaagccaa
      661 tgcttataaa tacactgaac gttacaccgc tttttatcgg gtgggtagtg gtacgcagta
      721 cactaaggac aaggacggaa atttagttaa ggttgccggt ggatatgctt ttaaaacagg
      781 aggaaccaca ggagttcctc tgatatctga tgcaacaata gtctctaatc ccgggcaaac
      841 ttataatcct gtaaacggcc ctttacctga ctatggagcc cctggggata gtggttctcc
      901 tttgtttgct tatgataaac aacaaaaaaa atgggttatt gttgctgtat taagagcata
      961 tgcaggtatt aatggtgcta cgaactggtg gaatgtcata ccaacagatt atctgaacca
     1021 ggttatgcag gacgatttcg atgcccccgt agactttgtt tccggactgg gccccctgaa
     1081 ctggacatac gacaaaacat caggcacagg taccctgagc cagggcagta aaaactggac
     1141 catgcacggg cagaaagaca atgacctcaa tgccggtaaa aatctggtat tcagcgggca
     1201 gaatggtgca attatcctga aagacagtgt gactcagggt gccggttatc tcgaatttaa
     1261 agacagttac accgtatctg ctgaatccgg aaaaacatgg acgggtgccg gcattattac
     1321 tgacaagggg acgaatgtaa cctggaaggt caacggcgtt gccggtgaca acttgcataa
     1381 gctgggggaa ggaaccctga ccataaacgg aacaggtgta aacccgggag gactgaaaac
     1441 gggagacggt atcgttgtac ttaaccagca ggcagacact gcaggtaata tccaggcctt
     1501 cagttcagtg aacctcgcca gcggacgtcc gaccgtggtg ctcggggatg cccgtcaggt
     1561 caatccggat aacatttcat ggggataccg gggaggtaag cttgacctta atggtaatgc
     1621 cgttaccttc acccgactgc aggctgctga ttacggggcg gtgattacaa ataatgcaca
     1681 gcaaaaatcc cagcttttac tggatcttaa ggctcaggat acaaatgtca gtgaaccgac
     1741 gattggaaat atatccccct ttggtggtac cggcactcca ggaaacctgt acagcatgat
     1801 actcaacagc cagacccgct tctatattct gaaatctgcc agctatggta acactctgtg
     1861 ggggaacagc ctgaatgatc cggctcagtg ggagtttgtt ggcatgaaca aaaacaaagc
     1921 agttcagaca gtaaaagata ggatcctggc cgggcgggca aaacaacccg ttatctttca
     1981 tggtcagctg accgggaata tggatgtcgc cattccacag gtgccggggg gaagaaaggt
     2041 aatctttgat ggtagcgtga acctgccgga aggtaccctg agtcaggaca gtggcaccct
     2101 gatattccag ggacatccgg ttatccatgc ctccatcagt ggcagtgcac cggtcagcct
     2161 gaaccagaaa gactgggaaa accgtcagtt tacaatgaaa acactgtcgc tgaaagacgc
     2221 tgacttccat ctttcacgta acgcctcgct gaacagtgac attaagtcgg ataacagcca
     2281 tatcacactg ggaagtgaca gggcatttgt ggataaaaat gacggaacag gaaattatgt
     2341 cattccggag gaaggtacct ctgtcccgga caccgtgaat gacaggagcc agtatgaagg
     2401 gaatattacg ctgaaccata actcagccct ggatatcggc agcaggttca ccggggggat
     2461 tgacgcttat gacagtgccg tcagcatcac ctctccggac gtcctgttga cagccccggg
     2521 tgcttttgcc ggcagttcac tgacagtgca tgatggcggt catcttacag cactgaacgg
     2581 tcttttcagc gacgggcata ttcaggccgg taagaacggc aaaatcaccc tgagcggtac
     2641 accggttaaa gatacggcta atcagtatgc ccctgctgta tatctgacgg acggatatga
     2701 cctgaccggc gataacgcag cactggaaat tacccgtgga gcacatgctt ccggtgatat
     2761 tcatgcctct gcggcatcaa cagttaccat cgggtctgac acgccggcag aactggcttc
     2821 tgcggaaacg gctgcatcgg cgtttgccgg cagtcttctt gagggctata acgcagcatt
     2881 caatggtgcc ataaccggtg gcagggctga tgtcagtatg cataatgcac tgtggactct
     2941 gggtggggac tctgccatcc acagtcttac cgtcagaaac agccgtatta gttctgaagg
     3001 agaccgtaca ttccgtaccc tgacggtgaa taaactggat gcaacaggca gtgattttgt
     3061 tttgcgtacg gacctgaaaa atgccgataa aattaatgtg actgaaaaag ccactggttc
     3121 agataacagc ctgaacgtca gctttatgaa taatcctgct cagggacagg ccctgaatat
     3181 tcctctggtc acggcaccgg cgggaacttc agcagagatg tttaaggccg gcacccgggt
     3241 gacaggtttc agtcgggtga ccccaaccct gcatgttgat accagtggtg gcaatacgaa
     3301 gtggatactg gatggtttta aagcggaggc tgataaagcc gctgccgcga aggctgacag
     3361 tttcatgaat gccgggtata aaaacttcat gacggaagtt aacaatctga acaaacgtat
     3421 gggtgacctg cgtgacacaa acggtgatgc cggtgcctgg gcgcgcatca tgagtggtgc
     3481 cggttctgca gacggtggtt acagtgataa ttacacccat gttcaggtcg gctttgacaa
     3541 aaaacatgaa ctggacggtg tggacctgtt taccggtgtc acgatgacct ataccgacag
     3601 cagtgcagac agccatgcat tcagcggaaa gacgaaatcg gtggggggcg gtctgtatgc
     3661 ttcagcattg tttgagtccg gtgcctatat cgatttgatt ggtaaatata ttcaccatga
     3721 caatgattac acaggtaact ttgctagcct gggaacgaaa cactacaaca cccattcctg
     3781 gtatgccggt gctgaaacgg gttaccgcta tcacctgaca gaggacacgt tcattgagcc
     3841 gcaggctgaa ctggtttacg gcgccgtgtc cgggaaaaca ttccgctgga aagacggtga
     3901 tatggacctg agcatgaaga acagggactt cagtccgctg gttggaagaa caggggttga
     3961 actgggcaag accttcagtg gtaaggactg gagtgtgacg gcccgtgccg gaaccagctg
     4021 gcagtttgac ctgctgaata atggagagac cgtactgcgt gatgcgtccg gggagaaacg
     4081 gataaaagga gagaaggaca gccggatgct gtttaatgtt ggtatgaatg cgcagataaa
     4141 ggacaatatg cgctttggtc tggagtttga gaagtcagcc tttggtaaat ataacgtgga
     4201 taatgcggta aacgcgaatt tccggtatat gttctgatgt gggggtaaag ggctgccgga
     4261 aggcagcctt tctcagagac tcttaaaata taaaactgaa ggtcttcggt aagggggagt
     4321 gtattagacg tcaactagtt ttgccgactg gcgcgagcct gtttaagatt ctgtgtaaat
     4381 gcctttctca gaagtgaccg tccaggcggt caccgaactc gataataaag cggctcattg
     4441 ccattcgcca gtccttcaac ggcatcgtcc atttctggga cgcagactgg attgccagcc
     4501 acaccacttt tttcaccgag tcgtctgtcg ggaacacttt acgctttttg atcgcatggc
     4561 ggatcacgct gtttagcgac tcgatggcat tcgtcgtata gatcactttg cggatgtccg
     4621 ttggataagc
//
LOCUS       DADLTY010000251         4622 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.255, whole genome shotgun sequence.
ACCESSION   DADLTY010000251 DADLTY010000000
VERSION     DADLTY010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.255"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(487..792)
                     /locus_tag="JN334_003992"
     CDS             complement(487..792)
                     /locus_tag="JN334_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439940.1"
                     /translation="MGSKIAGVVFLYFAVWGMASVIELQYWRNADTYPVWLTKHLRIS
                     PENNDKSLIRIEMETFDKVCGDKRGLLAVTQKQNGMFMRCDDSPGFFSWFSGVYKVN"
     gene            complement(846..1097)
                     /locus_tag="JN334_003993"
     CDS             complement(846..1097)
                     /locus_tag="JN334_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004182043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAY7439941.1"
                     /translation="MTNIILCDVTASVSELKNDPVATASARGGYPVEIIDRNRPVFYC
                     VPAALYEQMLDELDEKDLVQTITERQNQPLREVDLNQYL"
     gene            complement(1176..2447)
                     /locus_tag="JN334_003994"
     CDS             complement(1176..2447)
                     /locus_tag="JN334_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAY7439942.1"
                     /translation="MFALADVNSFYASCEKVFRPDLRGKPVVVLSNNDGCVIARSAEA
                     KRLGIKMGTPWFQLKEAQFPEKLYVFSSNYELYASLSNRVVALLEELSPRVEQYSIDE
                     CFLDARGISHCMDLEDFGRQLRGHVLSGTGLTIGVGFGATKTLAKSAQWASKEWPQFS
                     GVLALSPENPRRTAKLLSLQPVEEIWGVGNRIAKKLHVMGITTALQLSLTNPTFIRKN
                     FNVVLERTVRELNGESCISLEEAPPPKQQIVCSRSFGQRITTYGEMRQAVCQYAERAA
                     EKLRGERQYCRHISTFIKTSPFAVNEPYYGNVATEKLNTPTRDTRDIIAAAVRSLDRI
                     WLDGHRYAKAGIMLNDFSPNGVAQLNLFDDVQPRPHSDALMKVLDGINHSGLGKVWFA
                     GRGIAPDWQMKREMLSPAYTTRWKELPVARF"
     gene            complement(2447..2872)
                     /gene="umuD"
                     /locus_tag="JN334_003995"
     CDS             complement(2447..2872)
                     /gene="umuD"
                     /locus_tag="JN334_003995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY7439943.1"
                     /translation="MKFIPAVSEASIVKIPLFTERCPAGFPSPAANYTESELDLNEYC
                     IHRRHSTYFVRAIGNSMTDIGLYSGDLMVVDKAEQPRHGDIVIAEIEGEFTVKRLLLT
                     PRPALQAMNPDFPSLYPDPETLQIFGVVTAFIHKTRRAD"
     gene            2973..4193
                     /locus_tag="JN334_003996"
                     /pseudo
     CDS             2973..4193
                     /locus_tag="JN334_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISKox3 family transposase"
BASE COUNT         1091 a         1229 c         1221 g         1081 t
ORIGIN      
        1 gcacattatt agttagggac aacggttcaa tcaatgcctg tcacgaacag gtattggctt
       61 gtcgccagca acccgttgag cggagtaatc acgcagcagg acggctgtta caggcgatgg
      121 tgaaagctct gttgggatct gaacgaagca tgttgagtga aatttgcaaa atttcgcaga
      181 gtatgcggct tcatctttaa atttccgcca gcatttttaa agccagctac cgacgctgcg
      241 cgttggccgg cccttcgtcc ggcgggtaac cagtaccggc cgatcttacg accctggatg
      301 aacatcatcc cgtcagcagc gtctccactg tgatgtcgtt tttcacactg tccgatcaca
      361 aaatagaacg ggcagggaca acatatggta ggaaaagcag ctgccagaga cacaatatat
      421 tgattaccca cagtaacggg ggataaccat gtgggtaaca tattgaaagt gcaacacgtt
      481 atctgattaa ttgactttgt aaacgccgct gaaccatgaa aaaaaaccgg gggaatcatc
      541 acagcgcata aacataccgt tttgtttttg ggtgacggca aggaggcccc ttttatcacc
      601 acataccttg tcaaaggttt ccatttctat ccttatcagg ctcttgtcgt tattttcagg
      661 agagatccgg agatgttttg tcagccagac aggataggta tcagcgttac gccagtactg
      721 caattcaatc actgaagcca ttccccagac agcgaagtat aagaaaacca cacctgcgat
      781 tttgctaccc atattgttcc ttaacattgg cttaatacat tccttgctcc tcgcaccgga
      841 gcacatcaca gatactgatt gagatccact tcacgcagcg gctggttttg acgttctgtt
      901 atcgtttgca ccaggtcttt ttcgtccagc tcatcgagca tttgttcata caaagccgcg
      961 ggcacacaat aaaaaacagg gcggttacgg tcaattatct cgaccgggta gccgccgcgg
     1021 gcactggcgg tggccaccgg atcgttttta agttcgctga cgctggcagt cacatcacac
     1081 agaatgatat ttgtcatggt tcccccctga gacaagtcac ctgtatgatc ggtaaagtaa
     1141 gctctggcgg caagtccctt ctgtcatttc aacggtcaga aacgggcaac cggcagctcc
     1201 ttccagcgtg tggtatatgc cggtgacagc atctcccgtt tcatctgcca gtccggcgca
     1261 ataccccgtc cggcaaacca gactttcccg agtccggaat gattaatgcc gtcgagtacc
     1321 ttcatcagcg catcgctgtg cggccgtggc tgcacgtcat cgaacagatt cagctgcgcg
     1381 acgccgttcg gactgaaatc attgagcatt atccccgctt ttgcgtagcg gtggccatcg
     1441 agccatatcc tgtccagaga cctgaccgct gccgcgataa tgtcccgggt gtcacgggta
     1501 ggggtgttca gtttttccgt ggccacgtta ccgtaatacg gctcattaac cgcaaaaggg
     1561 gatgtcttga tgaaggtgct gatatgccgg cagtactgtc gctcaccgcg caacttttcc
     1621 gccgcgcgct cggcatactg gcagacggcc tggcgcattt ctccgtaggt ggtgatccgc
     1681 tgcccgaagc tgcgactgca gacaatctgc tgtttcggcg gcggcgcttc ctccagcgaa
     1741 atgcaactct cgccgttcag ctcacgtacc gtgcgctcca gcactacgtt gaagtttttc
     1801 cggataaatg tagggttcgt cagcgacagc tgcagggcgg tggtgatccc cataacgtgc
     1861 agctttttcg ctatccggtt ccccacgccc cagatttctt ccaccggctg caggctgagt
     1921 aattttgccg ttctgcgtgg attttcgggc gacagcgcca gcaccccact gaattgtggc
     1981 cattcctttg acgcccactg cgccgatttg gccagtgttt ttgtggcgcc gaagccgaca
     2041 ccgatcgtca gtccggttcc actgagaaca tgcccacgca gctgcctgcc aaaatcctcc
     2101 agatccatac agtggctaat tccccgggca tccaggaaac attcgtcaat tgagtattgt
     2161 tcgacgcggg gcgacagctc ttccagcagg gcgaccacac ggttactcag gctcgcgtag
     2221 agctcgtagt tgctggaaaa aacgtacagt ttttcaggaa actgcgcctc tttgagctgg
     2281 aaccaggggg ttcccatctt tatacccagc cgtttggctt cagcactccg ggcaataacg
     2341 cagccatcgt tgttactgag aacgacgaca ggtttgccgc gcaaatccgg cctgaaaacc
     2401 ttctcgcaac tggcgtagaa ggagttgacg tctgccaggg cgaacatcag tctgccctcc
     2461 gggttttatg aatgaaggcc gtcaccacgc cgaaaatttg cagagtttcc ggatccgggt
     2521 acagggaagg gaagtccggg ttcatcgcct gcagggctgg ccgcggcgtc agaagcaggc
     2581 gttttaccgt aaattcaccc tcaatttccg cgataacaat atccccgtgc cgcggctgtt
     2641 ctgctttatc cacgaccatc agatcgccgg aatagaggcc aatatcagtc attgagttgc
     2701 caatcgctct gacaaaatag gtggagtggc gccggtgaat gcagtactca ttcagatcca
     2761 gttcagactc agtatagttt gctgccggtg acggaaaacc cgcagggcat cgctctgtga
     2821 acaaaggaat ttttactatg ctggcttctg atactgcagg aatgaacttc atcgtttttt
     2881 ccctcgctta aaactgtata tgcatgggtc ttcccttgtt gtggtggctg aaggcatgat
     2941 aatggtgtat ttaatcgcca gaggtcaccg ccatggacga aaagtccctc tacgctcata
     3001 ttctcaacct gtccgatccg tggcaggtaa agtccctttc tctcgatgaa aatgccggtt
     3061 ctgttactgt cactattgag atcgctgaaa acacccggct agcctgtccg acctgcggta
     3121 aatcctgttc tgttcacgat caccgtcatc gtaaatggcg ccatcttgat acctgccagt
     3181 tcaccactat tgttgaagcc gatgttccac gaattatgtg tccggagcat ggctgcctga
     3241 cgttgcctgt tccgtgggct ggccccggaa gccggtatac gttgctattc gaatcgttcg
     3301 ttctctcatg gctgaaaatc agcaccgttg atgctgtcag gaagcaactt aagctcagtt
     3361 ggaatgcggt tgacggcatt atgacccggg cagttaagcg aggtcttgcc cggataaaaa
     3421 agccattatc cgcccgtcat atgaatgtgg atgaggtcgc ctttaaaaaa ggacatcgtt
     3481 acataacggt gatctccgat cgcgatggtc gggcgctggc cttaacggat gatcgcggca
     3541 cagagagtct tgccggctat cttcgcacgc tcactgatgg gcagttgctg gctatcaaaa
     3601 cgctctcaat ggacatgaac gcgggctata taagagcagc gcgtatccac ttacccagtg
     3661 cggttgagaa aatcgccttt aaccgcttcc atgtggcgaa gcaactgggc gaggtagttg
     3721 ataaaacccg tcagaatgaa catccgcacc tccctgttga aagccgacac caggcaaaag
     3781 gaacccgctt cctgtggcag tacagcgata agtggatgac cgaatcccgg caggaaaagc
     3841 tgatgtggct gcgtgcacag atgaagctga cgagccagtg ctgggcgctg aaagagctgg
     3901 caaaggatat ctggaacagg ccatggagcg aggaaagacg gagtgactgg cagagatggt
     3961 tggcgctggc ggctaacagt gacgttccca tgatgaaaaa tgccgcgaaa acgataggaa
     4021 aaaggctgta ggggatcctg aatgcgatgc gacacagtgt ctcaaacgga aatgcggagg
     4081 cacttaacag caagatcagg ctgctgagga taaaagccag gggataccga aaccgggagc
     4141 gctttaaact gggggtgatg ttccactacg gaaagctgaa tatggcgttc tgagccttcc
     4201 caccatgatc ggggaagacc catatgcata cagtataagg ggcggtgaaa tttctgcaag
     4261 tgatgggggg atagccggtc agaacgtccc cgaaaacaaa ccgcccccgg tttcaggaag
     4321 ggcgaactga ccggcagagg cagtgtaaca ccgcatgtca caaaggcagc actgacgtcg
     4381 tcagtgcttt ttttacccgt cctcccggac gctgaagggc agtttcaccc gtgcagctaa
     4441 aggggcggta agtccgggtg tgcacatcgt cattttctcc tgtgatgcct ttctcctgcg
     4501 tatcagcttc cggtaatcgt cccccgtcac aacctggaca gcaagggtaa atggcacgct
     4561 gcgcgccctt gctgaccaga acgactccgg gcaggccgaa ccgtcaggcg atacgaagac
     4621 ga
//
LOCUS       DADLTY010000252         4602 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.256, whole genome shotgun sequence.
ACCESSION   DADLTY010000252 DADLTY010000000
VERSION     DADLTY010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.256"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..1321)
                     /gene="norR"
                     /locus_tag="JN334_003997"
     CDS             complement(<1..1321)
                     /gene="norR"
                     /locus_tag="JN334_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HAY7439944.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVT"
     gene            1508..2947
                     /gene="norV"
                     /locus_tag="JN334_003998"
     CDS             1508..2947
                     /gene="norV"
                     /locus_tag="JN334_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HAY7439945.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            2944..4077
                     /gene="norW"
                     /locus_tag="JN334_003999"
     CDS             2944..4077
                     /gene="norW"
                     /locus_tag="JN334_003999"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HAY7439946.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(4205..>4602)
                     /locus_tag="JN334_004000"
     CDS             complement(4205..>4602)
                     /locus_tag="JN334_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="carbamoyltransferase; involved in the formation of
                     the cyanate group of hydrogenase; catalyzes the formation
                     of carbamoyladenylate from carbamoylphosphate and then
                     transfers the carbamoyl group to HypE; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAY7439947.1"
                     /translation="CALEALAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQ
                     RAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQ
                     SLPAGDGGLSLGQGVIAAARWLAGEVQNG"
BASE COUNT         1148 a         1306 c         1203 g          945 t
ORIGIN      
        1 acgtcacctc aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg
       61 tggctctcgc cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg
      121 gaaaactgta gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc
      181 cctgccgcaa acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc
      241 gctcacgcag cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc
      301 ggaatcgccc tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca
      361 catcgacccg caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc
      421 tcagcagctt ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac
      481 cgttatccgc catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt
      541 tcacatgccc gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat
      601 agaccagcgg attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct
      661 taccggttcc cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa
      721 tctctttttt cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg
      781 cttcaaacgg cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca
      841 acgcattgct taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga
      901 aaacatcgaa ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc
      961 cgacaaacaa tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca
     1021 aaccgtcata gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc
     1081 gggcaatcgc ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct
     1141 ttgccagacc gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag
     1201 acgcatcgca ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat
     1261 gaccaatccc acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca
     1321 tctttgcctc actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt
     1381 cattttgact actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag
     1441 aaactggcac gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga
     1501 ggttgctatg tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga
     1561 agtgcgtgat tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta
     1621 cctcatccgc gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga
     1681 atttgtgcag aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa
     1741 ccatgcagaa gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac
     1801 gccgatctac tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga
     1861 gtggaatttt aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct
     1921 catttttgtc gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg
     1981 cgacgcggtg ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt
     2041 caacgatgaa gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat
     2101 cctgacgccg ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt
     2161 accagtcgat atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat
     2221 tgtcgagctg tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta
     2281 cgacaccatg tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga
     2341 aaccgaccca cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat
     2401 cctgaccaat gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt
     2461 gatgatgccg aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa
     2521 acgcgccagt gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac
     2581 gcgcctgcag gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga
     2641 ccaggacgct ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct
     2701 cgcgccgctg ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac
     2761 gacggctgac ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc
     2821 aaaaggcgag ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa
     2881 cttcctctgc ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc
     2941 aaaatgagca acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa
     3001 aatattcgca aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat
     3061 gagtacaaca aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt
     3121 acccgccaga cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc
     3181 tgggtgacgg atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa
     3241 tacgacaagt tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt
     3301 gagttaatgc tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg
     3361 gatgcccgac gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat
     3421 ttttgtcgtg caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca
     3481 ttaatgccac cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat
     3541 ctgctgttga aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg
     3601 ctggaccacc agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg
     3661 gaaacagccc tagcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt
     3721 tatctgcaaa ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt
     3781 caggtattgc cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt
     3841 ctcggcaata acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa
     3901 ttaccgctgc atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc
     3961 gaacgccagg gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg
     4021 gtcagtgagg atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg
     4081 ggctactgtg cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac
     4141 gcatgtgtag gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca
     4201 cgctttatcc gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc
     4261 ccagagacaa accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag
     4321 cgagataatg cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata
     4381 ccagcgtagt gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg
     4441 ccagcgcatc atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact
     4501 gctgccagaa agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg
     4561 tcactccgtc gcatgaggct gcgagcgcct ccagagcaca ag
//
LOCUS       DADLTY010000253         4602 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.257, whole genome shotgun sequence.
ACCESSION   DADLTY010000253 DADLTY010000000
VERSION     DADLTY010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.257"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(78..1001)
                     /gene="phoH"
                     /locus_tag="JN334_004001"
     CDS             complement(78..1001)
                     /gene="phoH"
                     /locus_tag="JN334_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HAY7439948.1"
                     /translation="MRSRFSAFYPKANYGLQGSQPSDVRAHNRAANGACDEYKQLKVL
                     SMGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEAIGMARDSRDTSPI
                     LARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHKDVDRIIVTRPVLQ
                     ADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIGKVETAPFAYMRGR
                     TFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLPRGVCSGLSDALER
                     FEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="JN334_004002"
     CDS             complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="JN334_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HAY7439949.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2744..3871)
                     /locus_tag="JN334_004003"
     CDS             complement(2744..3871)
                     /locus_tag="JN334_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HAY7439950.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3929..>4602)
                     /locus_tag="JN334_004004"
     CDS             complement(3929..>4602)
                     /locus_tag="JN334_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HAY7439951.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1116 t
ORIGIN      
        1 ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct
       61 ctgcaccgat aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt
      121 acgcaatcct cttttccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc
      181 agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg
      241 ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca
      301 gtcacattct gcgcctcgtc aagaatgaca actgcatttt caaaggtacg tccacgcata
      361 taggcgaacg gcgcggtttc caccttccct atttccggtc gcaggcagta ctgcataaag
      421 gaagccccta agcgccggac cagcaagtcg tagaccgggc gaaaataggg agcaaacttt
      481 tctgcgatat caccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg
      541 acgataatcc tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc
      601 caggtttttc cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc
      661 tgcagatagt gcaattgcgc ttcatttcgc gcaaggatgg gcgaggtatc gcgactgtcg
      721 cgggccatac caatggcttc tacgccgctc atctgcacaa gcgaggtgac cgattcttct
      781 tcacgctgct tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct
      841 ttgatcactg ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac
      901 gcgccgttgg cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca
      961 tagtttgctt ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta
     1021 acacgagtaa agaaaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga
     1081 aatctccgcg agtggtggag cgttgcttaa caaaacattg tccagaacag gacgttacca
     1141 tccgcgatct ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataag
     1201 tacaacagaa ctcgcattca ttgcaacaat atattcactt atatttaacg ataacaccca
     1261 tttcggtgac attaatttct actggaaaga tgacagagtg atgacagtga tggaaaaagc
     1321 tgtgtgcttt ccgcaggatt cgcagggctg gtaaaatgtg cctcgtgttt gccggattcg
     1381 gcgcgaacgc cttacccgac ctacaagtga ttaataattt gtgcacaaaa ctcatgcccg
     1441 ataaacctgc gcatcgggca tatttgcgtc gttcaaacct gcaatagcgc ccttcccaga
     1501 taatcgttcg cgtccttcac tcccggcagc gaaaaaaaat aaccgccgcc gataggttta
     1561 acgtattcct ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc
     1621 agatcgtgtt ggtagcagac aaacagcaac cccatatcca gttgtccgga gttggtgacc
     1681 cccaacgaat aactgtagcc gcgacgcagc atcaggctgg actcactctc cgccgtgcgg
     1741 ggattcgcca gccggatatg gctgtccagc gcgatcacct tcccttccgg gtcgctggcg
     1801 taatcaggca catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacgg
     1861 ccaaaaatcg tctgctgttc tttcagcggc gttctgtccc aaaattccac tcgaaactga
     1921 atcaagcgta ctgcctgata gctgccaccg attgtccacg caggctcctg ctgatctgcc
     1981 gttacccaca ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg
     2041 tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg
     2101 gaaataaacc cttcccgctt ccagcgcaca ctgagcaaat ccggcgtgtg tttgatgata
     2161 tcgcgcagcg catggttcac cgtgtcctgg gtgttggcgc aaatctgtag caacacatca
     2221 ccatgacata acgccgcatc cagcgagtcg ttggggaaac gcgtcatctt ctgcagcttt
     2281 tttggcatct gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc caccgataac
     2341 gtgatggtga gattatcggg cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc
     2401 aggcgcggat ttggcgtttc tggtgctgct ccgccctgac tcagaaaagc aaaacgctga
     2461 gtcaacaagc gaaacaaccg ctcaagatcg gctttatcgc tggcaagcac atcaaacgcc
     2521 accagcatca ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac
     2581 ggctgtttct cattgcgtgc atccggtgaa agcgtgcccg gtgcactttg cgttttttgt
     2641 gcatgagcga ccggacaact tcccgccagc gcaccaatcc ctttcagtaa acgtcggcgt
     2701 gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca
     2761 caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggccctttc agcgcattcc
     2821 ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca
     2881 gaatggtatc gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt
     2941 tttgcagttg tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga
     3001 aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca
     3061 cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac
     3121 tgatgcgttt ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc
     3181 ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg
     3241 ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg
     3301 agaacagttc agcaatcggt tcaatacgct catagtgctg gcgcgtcggc gcatacagtg
     3361 ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca
     3421 ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac
     3481 ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc
     3541 ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg
     3601 tcattttctg gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt
     3661 tgaggatctc ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc
     3721 cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct
     3781 gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact
     3841 gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt
     3901 aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc
     3961 gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt
     4021 cgctgacgct cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg
     4081 agtgagttga gaggaccgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc
     4141 cggcttcatg aaatgcgcga atggcaccag ccgccagccc tgcggcgacg aagagaataa
     4201 acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca
     4261 ggaagccgag caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc
     4321 cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa
     4381 cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc
     4441 tatcgactgc ctgttccagt tgcactttga cgttgcgcga cactttgcgc atccagaaaa
     4501 ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgct
     4561 ctttttgcgg aaattcgccg gtggtttcgt taatgaagat cc
//
LOCUS       DADLTY010000254         4577 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.258, whole genome shotgun sequence.
ACCESSION   DADLTY010000254 DADLTY010000000
VERSION     DADLTY010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.258"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            5..1138
                     /gene="blaEC"
                     /locus_tag="JN334_004005"
     CDS             5..1138
                     /gene="blaEC"
                     /locus_tag="JN334_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY7439952.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1227..1760
                     /locus_tag="JN334_004006"
     CDS             1227..1760
                     /locus_tag="JN334_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY7439953.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1757..2074)
                     /locus_tag="JN334_004007"
     CDS             complement(1757..2074)
                     /locus_tag="JN334_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAY7439954.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2250..2396)
                     /gene="ecnB"
                     /locus_tag="JN334_004008"
     CDS             complement(2250..2396)
                     /gene="ecnB"
                     /locus_tag="JN334_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY7439955.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2507..2632)
                     /gene="ecnA"
                     /locus_tag="JN334_004009"
     CDS             complement(2507..2632)
                     /gene="ecnA"
                     /locus_tag="JN334_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY7439956.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(2684..3250)
                     /gene="efp"
                     /locus_tag="JN334_004010"
     CDS             complement(2684..3250)
                     /gene="efp"
                     /locus_tag="JN334_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY7439957.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3292..4320
                     /gene="epmB"
                     /locus_tag="JN334_004011"
     CDS             3292..4320
                     /gene="epmB"
                     /locus_tag="JN334_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY7439958.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1178 a         1164 c         1135 g         1100 t
ORIGIN      
        1 ccttatgttc aaaacgacgc tctgcgcctt attaattacc gcctcttact ccacatttgc
       61 tgcccctcaa caaatcaacg atattgtgca tcgcacaatt accccgctta tagagcaaca
      121 aaagatcccc ggtatggcgg tggcggtaat ttatcagggt aaaccttatt actttacctg
      181 gggctatgcg gacatcgcca aaaagcagcc cgtcacacag caaacgttgt ttgagttagg
      241 ttcggtcagc aaaacattta cgggcgtgct tggtggcgac gctattgctc gaggggaaat
      301 caagttaagc gatcccgcaa caaaatactg gcctgaactt accgctaaac agtggaatgg
      361 gatcacacta ttacatcttg cgacctacac cgctggcggc ctgccattgc aggtgccgga
      421 tgaagtgaaa tcctcaagcg acttgctgcg cttctatcaa aactggcagc ctgcatgggc
      481 tccaggaaca caacgtctgt atgccaactc cagtatcggt ttgttcggcg cactggctgt
      541 gaagccgtct ggtttgagtt ttgagcaggc gatgcaaact cgtgtcttcc agccactcaa
      601 actcaaccat acgtggatta atgtacctcc cccagaagaa aagaattacg cctggggata
      661 tcgcgaaggt aaggcagtgc atgtttcgcc aggggcgtta gatgctgaag cttatggtgt
      721 gaagtcgacc attgaagata tggcccgctg ggtgcaaagc aatttacccc cccttgatat
      781 caatgagaaa acgcttcaac aagggataca actggcacaa tctcgctact ggcaaaccgg
      841 cgatatgtat cagggcctgg gctgggaaat gctggactgg ccggtcaatc ctgacagcat
      901 cattaacggc agtgacaata aaattgcact ggcagcacgc ccggtaaaag cgattaagcc
      961 cccaactcct gcagtacgcg catcatgggt acataaaacg ggggcgaccg gcggatttgg
     1021 tagctatgtc gcgtttattc cagaaaaaga gctgggtatc gtgatgctgg ctaacaaaaa
     1081 ctatcccaat ccagcgagag tcgccgccgc ctgtcagatt ctcaacgctc tacagtaaaa
     1141 tttcatcggg tccgaatttt cggacctttt ctgcgctttt ccttgctgtc atctacactt
     1201 agaaaaaaac cagtaaggaa acatttatgc gcctgctccc tctcgttgcc gcagcgacag
     1261 ctgcatttct ggtcgttgcc tgtagttttc ctacgccgcc gcgtggcgtg accgtagtga
     1321 ataatttcga cgccaaacgt tatctcggta cctggtatga gattgcccgt tttgatcacc
     1381 gttttgaacg tggactggaa aaagtcaccg caacatacag cctgcgtgat gacggcggcc
     1441 tgaatgtcat taataaaggc tataaccctg acagaggaat gtggcagcag agtgaaggga
     1501 aagcgtactt taccggcgca ccaactcgcg ctgcgctgaa agtgtcattc tttggtcctt
     1561 tctatggcgg ttataacgtt attgcactcg atcgggaata ccgccatgcg ctggtttgcg
     1621 gcccggaccg cgactacctg tggatactct cccgcacgcc aaccatttct gacgaagtga
     1681 aacaggagat gctggcagtc gcgacccggg aagggtttga tgtcagtaag tttatttggg
     1741 tacagcagcc cggtagttag tgagtgctga gtttcagacc aataatcccc aatacgatta
     1801 acgccagact cgccagacgc atcgggttag cggactcacc gagcagcaca atgccggtga
     1861 tggccgcgcc gactgcgcca atacccgtcc acacggcata agccgtccct accggtaacg
     1921 atttcatcgc ccaggcaagt agcgccatac tgactatcat cgccgtcacg gtaataacac
     1981 tcggcgtcaa acggctaaag ccgtgggtat atttcaggcc aacggcccat accacttcca
     2041 gcagaccagc aataactaag ataatccagg acatatcagg ctccggaaat ttggggccgt
     2101 ccccggtgaa aagaggcgtt tgcaggtcgt cctgcaaagc tgatggttga acggatattt
     2161 tgccaggtgg caggggaatt tcaactatta cctttatgac gatcgcagaa aaaacaaacg
     2221 gcacgacact gctgtcgtac cgttattgct tattgctgcg ctttcgttgc tgcgccagaa
     2281 atcgcgttac cgccatcaga aatgtcttca ccaacgccac gcgtggtgtt gcaggcagtt
     2341 aatactgttg aaagcaccag aacagaaaag atcgctgcaa ttgtcttctt caccataacg
     2401 tcttcctttt aggtaatgtt atttatgttt gcctatagca aattaataat agacaacatc
     2461 gccaaaaatg atggaaatct gatgattttt cggaagagaa gaaaaattag ctggcagcgc
     2521 gagagatgga gttaccgaga tgtttgatgt cttcgccgaa accgcaagcg gtgttacagc
     2581 ccgtgagcag cgtgctggca agcaaaacaa gaacgataag gcgtttcatc atctctgacc
     2641 tgaataagtg atggtgcagc ctgcaggccg caccacaacc gcattacttc acgcgagaga
     2701 cgtattcacc agagcgggta tccactttga tgacttcgcc gatttgtacg aacagcggaa
     2761 ctttaaccac agcgccagta gacagggtag ccggtttgcc gccagtacct gcggtatcac
     2821 ctttcaggcc cggatcggta tcaacgattt ccagttcaac gaagttcggc ggagtaacgg
     2881 agatcggctg accattccac agagttacga tacactctgc ctgatccagc agccatttag
     2941 cattgtcgcc aattgctttt gcatcagcag acagctgctc gaaagtttcg ttgttcatga
     3001 agtgccagaa ctcaccgtcg ttgtacaggt aagtcaggtt catatcgaca acatcagcgc
     3061 cttcagcgga atcagtagat ttgaaggttt tttctacgcg agtaccggtc agcagacgac
     3121 gcagtttaac gcgagcaaat gcctggcctt tacccggttt tacgaattca ctcgcttcaa
     3181 ccgcgtaagg ttcgccgtct aacatgattt taagaccagc acgaaaatcg ttgctatagt
     3241 acgttgccat aaggccctct gaaatttgtt aattggtagc taagccacaa aatggcgcat
     3301 attgtaaccc taaatacccc atccagagaa gattggttaa cgcaacttgc cgatgttgtg
     3361 accgatcctg atgaacttct gcgtcttttg aatatagacg cggacgaaaa actgttggcc
     3421 ggacgcagcg ccaaaaagct gtttgccctg cgtgtgcccc gctcatttat cgatcgcatg
     3481 gagaaaggca atccggacga tcctcttttg cgtcaggtac ttacctcgca agatgagttt
     3541 gtcgtcgcgt ccggattctc caccgaccca ctggaagaac agcacagcgt agtgcctggt
     3601 ttgttgcata aataccacaa ccgggcgctt ttgctggtca aaggcggctg cgcggtaaat
     3661 tgccgctatt gcttccgtcg ccacttcccc tatgccgaaa atcagggcaa caagcgtaac
     3721 tggcagactg cacttgagta tgttgctgcg catccggaac tggacgagat gattttctcc
     3781 ggcggcgatc cgctgatggc gaaagagcac gagctggact ggttgctcac acaactggaa
     3841 gccatcccgc atatcaaacg tctgcggatt cacagccgtc tgccgattgt gatcccggca
     3901 cgtatcaccg aggcgctggt tgaacgcttt gcccgttcta cgctgcaaat cttgctggtg
     3961 aatcacatca atcatgccaa tgaggtagat gaaacgttcc gtcaggcgat ggctaaattg
     4021 cgccgtgtcg gtgtcaccct gcttaaccag agcgttctgt tacgtggtgt gaacgataac
     4081 gcacaaacgt tggcaaacct gagtaatgcg ttgttcgatg ccggcgtgat gccgtattac
     4141 ctgcatgtgc tagataaagt acagggcgcg gcgcatttta tggtgagtga tgacgaagca
     4201 cggcagatta tgcgtgagtt gctgacattg gtgtcgggat atctggtgcc gaaactggcg
     4261 cgagaaattg gcggcgaacc cagcaaaacg ccgctggatc tccagctacg ccagcagtaa
     4321 ctgattaaac agggaagtta aacattaaaa ttgcctgatg cgctacgctt atcaggccta
     4381 catcgtgcat tgaaattgaa tggatttgca atgaactgta ggccggataa ggcgtttacg
     4441 ccgcatccgg caagagcaac aaacgcaagt acagcaacca ctgagaaatt taatctttgc
     4501 acgttgcata acctttaatt attcatatgc attgctgttg ttacttttta tatagctaac
     4561 accaataatt aaccttt
//
LOCUS       DADLTY010000255         4554 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.259, whole genome shotgun sequence.
ACCESSION   DADLTY010000255 DADLTY010000000
VERSION     DADLTY010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.259"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..158)
                     /locus_tag="JN334_004012"
     CDS             complement(<1..158)
                     /locus_tag="JN334_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY7439959.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="JN334_004013"
     CDS             complement(308..469)
                     /locus_tag="JN334_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439960.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(466..669)
                     /locus_tag="JN334_004014"
     CDS             complement(466..669)
                     /locus_tag="JN334_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY7439961.1"
                     /translation="MLERTMAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAIT
                     PYTLMQAIAAEGDVVVSGATEPE"
     gene            914..1249
                     /gene="iraM"
                     /locus_tag="JN334_004015"
     CDS             914..1249
                     /gene="iraM"
                     /locus_tag="JN334_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY7439962.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1661)
                     /locus_tag="JN334_004016"
                     /pseudo
     CDS             complement(1530..1661)
                     /locus_tag="JN334_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611307.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 domain-containing protein"
     gene            complement(2161..2237)
                     /locus_tag="JN334_004017"
     tRNA            complement(2161..2237)
                     /locus_tag="JN334_004017"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="JN334_004018"
     tRNA            complement(2339..2414)
                     /locus_tag="JN334_004018"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="JN334_004019"
     CDS             complement(2559..3380)
                     /locus_tag="JN334_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7439963.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="JN334_004020"
     CDS             complement(3395..3751)
                     /locus_tag="JN334_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7439964.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4554)
                     /locus_tag="JN334_004021"
     CDS             complement(3764..>4554)
                     /locus_tag="JN334_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY7439965.1"
                     /translation="RHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLRDQTSE
                     SLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVLRRSLG
                     LRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDP
                     ESPESFMKRPKRRRWVNEKYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIF
                     TLPLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1240 a         1235 c          919 g         1160 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgcc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaaaacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgggttctg ttgctggtgg gcgtgaggca
     4141 gcggcgcaag attttttgtg cgctgcttca gtatgctggt ggcggtctgc tctcccggca
     4201 cgatgtcgct ttcacggtac attgagcgga ttttttccgc acgcaacccc agcgaacgac
     4261 gtaataccgc ttccggtagc gcgtccgcca cctgattgcg gaccgcccac caggataatt
     4321 cagccagaga taattcacgc tcctgcgtac cgcttattgc gtgaccgatg acgtcaatca
     4381 tccatgctga caggttttga tgagcaagtt gctcgagtga ttcggatgtc tggtcacgca
     4441 gctggttgtc gcagtgccag cacaatacca ttgcgccggt accataacgg tgaatgacgg
     4501 tttcgctgtg atgataatcg ccgtgtggcc actggcagga tttaatatgg cgca
//
LOCUS       DADLTY010000256         4549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.260, whole genome shotgun sequence.
ACCESSION   DADLTY010000256 DADLTY010000000
VERSION     DADLTY010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.260"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..1223
                     /locus_tag="JN334_004022"
     CDS             <1..1223
                     /locus_tag="JN334_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAY7439966.1"
                     /translation="PYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLYP
                     LSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGISN
                     YAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGVT
                     NIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEKW
                     FIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILPV
                     IVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHLL
                     IANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRAA
                     RVFVNDERFTRGGN"
     gene            1223..1957
                     /locus_tag="JN334_004023"
     CDS             1223..1957
                     /locus_tag="JN334_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7439967.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2425..3186
                     /locus_tag="JN334_004024"
     CDS             2425..3186
                     /locus_tag="JN334_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7439968.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3230..4309
                     /locus_tag="JN334_004025"
     CDS             3230..4309
                     /locus_tag="JN334_004025"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7439969.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4306..>4549
                     /locus_tag="JN334_004026"
     CDS             4306..>4549
                     /locus_tag="JN334_004026"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY7439970.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT         1247 a          863 c         1120 g         1319 t
ORIGIN      
        1 ttccgtatgg cattttccag cagcgaaatg ttcttgcgag ctggctggca acaggttgcg
       61 gcgtagcgtt gtatctggct ttaacggcgc gtactcgttc tctgacgttg atatgtttgt
      121 tgtcgttata ccctctgtct acagctttag tccttacaca aagccgtgtc ggccttcttg
      181 gtttattttg cgtcatcgtg ctggccgctc tcgcggacag accccgctgg cgcggacgtc
      241 cccttagcgg tctgttgcgc ttgatcttcg tcgcctcctt gctgtgctgg tgtgtcggta
      301 tctcaaacta tgccatgcca ggggagacaa cggctgactt tacgcattca tcatcgaatg
      361 accagcgtat acgtattctg aaaggtgcca taagtcttat tcaacagcgc cccttaaccg
      421 gttgggggtt aggcagcttt gaagcgacct tcccgcaagc cttagaggat gtgggcgtga
      481 caaacataga gagtgatacg gctacgcatc cccataacga actgctgttc gtaacggctg
      541 aaggtggcat cgtcgccttg tttggttttt tgctgctatc aggcgtttgg ctgtggccgt
      601 gtatatgccg cctgaaaatg acaaacaata acgcgcacca tcaatgtgaa aagtggttta
      661 ttgccttaat ggggttgcct ttagtcattc atatgatgac cgaatatcct ctctatacgt
      721 ctgttcccca tttgatgctg ttgctgctcc ttttccgcat cggaatgccg gaaaacgtga
      781 tgaatcaggt acgtatacct aaaacagtcc ggttgggtat tctgcctgtc attgtgctca
      841 ttatgaccgc cgcgctggtc atcctggtag cgggatttga tgcacagcgg gaactgacca
      901 gagctgaagc tgatatgaac gaaggattta tccctcgcct tccggaacat agctggaaat
      961 acctgacgca ggcagaacgt cttgagcgcg atcagcatct gcttattgcg aataaaccgg
     1021 ggttcgttcg cgatacggaa agcatgtctc ttttcactgt gtggggaaat aaatggttgg
     1081 caatccacaa tgatgcagac gtaagcgctg caatgatatt cattgcgcac aggcgcggtg
     1141 attatctgac agctgaaaaa ataagaaaac gcgcagctcg ggtatttgta aatgacgaac
     1201 gttttactcg cgggggaaac taatggttaa cgatctgacg tataagaaat tagaaagcac
     1261 aaacatcaac tgtaatattc tctcgccaga aaataccaca tggacaaatg tcacggtgat
     1321 tggcctggat ctttttgcca tttgcggcgt gtgcagtttg ctcaagcgtg ttgggggagt
     1381 gaaacttcag gttttcactt ccttacccac tgatatgaat gttctgactg acagtagcat
     1441 cgtgatatgg gtaaaaatgc gtcacgatgg tttgcctgaa ttagccggac atattgtgaa
     1501 ggtttgtcgt cgataccgtc tgatgcggca actcgttatt agcgatgcta ttcctgaagg
     1561 aatgacgaat gatatcgggc ctctttcccg agtatggctt actcatggga gtgcaaaagt
     1621 ggatatttta ttatcttcat tacggaaggt gttaaatgct cattgtttgc ctggtccttt
     1681 gtttataaaa cgcctgggaa gagtgcaatg gagggttctt ttactccgcg cgaaggggat
     1741 tagcacacgt aaaattgcag aaatctgtgg tattggcctc aagaccgtta gcgcccatga
     1801 gtccgcaatt cgagaacgat taggcaccag gaataaaagt gagtacgtct ggttagtacg
     1861 taatgctcaa cttatacaac aggcgatacc cgcgttggga agagatgcgt caaggtatga
     1921 aagatgtaac aatatgcgaa caaagcaaaa agtataattg atgcgtcata gataactctc
     1981 tttttcgatg acgaaatcgc tgagcatgcg tggtgaggtt agtaatagat gggtgctatg
     2041 ttttgccacg ggtttgtagc tgtgaaacct ggaaaaaaat aagaattaaa aatatttaaa
     2101 gagagttaat aaggttctgg ttgtaattgt cttggtcaaa aaaaagatat tgtcaatgtt
     2161 gttttttaaa ttttcaggtt ttggttaaat atttgcttgc aaatataggt gattttttta
     2221 ttgtaggctt ttgaaggcta ttataactgt gcgctaaatt aatgcgttga ataaaagaaa
     2281 taacaaaatt aagaaaagcc atatttacga tatgggcaac ataaatataa tctaaaaaag
     2341 agtaaggcgt tgtaggttgg ttccgcgttc attatcttgt tagtgaacgc ggatttatca
     2401 gttgtcgaaa tggtttttat aataatgaaa aattttaatg tactaaccaa agtcgtattt
     2461 tgcttgtcgt ttttatcaag cgcatttccc acctatgctg cggatgatgt caaagtgact
     2521 caaaacactt cagtttttaa actgaagctt agtgcaacgc gattaatcta tgaacaaggg
     2581 tcggatggcg cagcgataac tgtgacaaat ccgcaggatt ggccgatgct tgtgcagagt
     2641 aaagtctttg aagaagataa agtaactgca gcgcccttta ttgtcactcc gccactgttc
     2701 aggcttaatc caggccagca aaataaaatg tgggttgtgc agactgcgcc agctttacaa
     2761 gacagggaaa aattacactg gctatgcgcc accggcatta cgcctaaaaa gagtgatgtt
     2821 tgggccgaga aagataaaga aaaaattgct gaaaatgtga acctgaacat ccaggttaac
     2881 gtacatacct gcataaaact tattaccagg ccatctggta ttgaaggcaa gatggttaat
     2941 gcgagtgaga agttatcgtg gaaaaagcag ggtgataaaa ttgtcgcggt caatcagtcg
     3001 ccgttctaca tcaacctttc ttctttacag gttggcggta gcaagattga gaaactagat
     3061 tatattgcgc cgttttcgca acatgagttt gttatgccaa aaactagcgg ccaggatatt
     3121 aaatggtctg tgattactga tgaaggtggt gagagtcgtt tgtttatatc tcatttgcaa
     3181 tcataatgta ttacattttt attgaatatt tttatgccat gcatattcca tgttttatcg
     3241 actgtcatta attttgataa tggccttact tgcgggtcag ttaagtgccc aggagtggtc
     3301 atttgattct tcacagttgg aaggaaacgt tagtgcggac actgtggcaa tgtttaatca
     3361 gggagagcaa ctccccggaa attatcgtgt ggaaatttat cttaacggtg aaaaagttga
     3421 tgttggggag tttccctttc atcgtccgga atcccctgaa gaaaaagagc ttgttccatg
     3481 tttaaccgtt gatgacctta tccactatgg cattaaaatt gataaaagca gcagtgatac
     3541 tgacaataaa aagaatcagt gttttaaatg gaactctatt gaaggtttga aagtaaatta
     3601 tgattttgat agccagcgcg tacagataac cgttccacaa ctgtatctgc aagataaaaa
     3661 aagtagttta gcaccagtgt cgttatggaa tgagggtgta gctgcattcc gcatggtgta
     3721 tcagacaaat attgatattt ccaaacaaaa tgataatcaa tctaccacgc gtaattctcg
     3781 ttatggcagg ttcactccag ggtttaatct tggtgcctgg aggtttcgta gttcagtgac
     3841 atggagcaag gaacttggcc agtcagaaag atggcaaagg ggttacatgt ggtttgagcg
     3901 aggcataaat gcgattaaaa gccgattaac gcttggagaa tcttatacgt cgtctgaggt
     3961 gtttgacagc ataccttttc gcggcgggat gttagcgacc gacgatgcga tgactccgcc
     4021 ggaggacagc tactacacac ctgtggtgca tggaatagca caaagtgagg cgcaggttat
     4081 tattaaacaa aatgggcaaa ttatttttac ccgttctgtg ccgccagggc cttttgcgct
     4141 ggataattta ccaacgctgg ccgttggcgg ggagcttgat gttacagtga gagagagtaa
     4201 tggcgaggag cagtattttt ccgtgccatt tcaaacgccg gccatcgctc tgcatgaagg
     4261 atatttcaaa tatagtgtga tgggggggaa tataaagaag aaggtataaa aagctcccct
     4321 gtctccgtgg gcgaaatcac ggcgatgtac gggcttcctt atggcgtcac ggtgtatgga
     4381 gggattcaaa acgccactca ttttaatgca atatctacgg gtattggcat ttctctgggg
     4441 ctgttaggga gtttatcgac agatattacg cgttcgattg cgaatttata ttatgggaat
     4501 aaatatcgta ttcgttatag taaatccatt agtgattttg gtactcaat
//
LOCUS       DADLTY010000257         4546 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.261, whole genome shotgun sequence.
ACCESSION   DADLTY010000257 DADLTY010000000
VERSION     DADLTY010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.261"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..474
                     /locus_tag="JN334_004027"
     CDS             <1..474
                     /locus_tag="JN334_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7439971.1"
                     /translation="LMLEPQLQYTWQGLSLDDGQDNASYVKFGHGSAQHVRAGFRLGS
                     HHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNM
                     TFSPSQNGTSLDLQAGLEARVRENITLGVQASYAHSINGSSAEGYNSQATLNVTF"
     gene            595..3111
                     /locus_tag="JN334_004028"
     CDS             595..3111
                     /locus_tag="JN334_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439972.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            3187..3642
                     /locus_tag="JN334_004029"
     CDS             3187..3642
                     /locus_tag="JN334_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439973.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            3743..3874
                     /locus_tag="JN334_004030"
     CDS             3743..3874
                     /locus_tag="JN334_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAY7439974.1"
                     /translation="MAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIRNAEGQRR"
     gene            4053..>4546
                     /locus_tag="JN334_004031"
     CDS             4053..>4546
                     /locus_tag="JN334_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAY7439975.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEE"
BASE COUNT         1096 a         1159 c         1229 g         1062 t
ORIGIN      
        1 ctgatgctgg agccgcaact gcagtacacc tggcagggac tctccctgga tgacggccag
       61 gataacgcca gttatgtgaa gttcgggcat ggcagtgcac aacatgtgcg tgccggcttc
      121 cgtctgggca gccaccacga tatgaacttt ggtaaaggca cctcatcccg tgacaccctg
      181 cgcggcagtg ccaaacacag tgtgcgtgaa ctgccggtga actggtgggt acagccttct
      241 gttatccgca ccttcagctc ccggggggac atgagcatgg gtacagccgc agccggcagt
      301 aacatgacgt tctcaccgtc acagaatggt acgtcactgg acctgcaggc cggactggaa
      361 gcccgtgtcc gggaaaatat caccctgggc gttcaggcca gttatgccca cagcatcaac
      421 ggcagcagcg ctgaaggtta taacagtcag gccacactga atgtaacctt ctgataattc
      481 tgcattgcct ctctgtggtc ccggtcatga tgaccgggac ccggacgggg gaaaacgttt
      541 cagtgccaca ttcactggca ttcacaataa catgatattc atcacggagt gactatgtta
      601 cagatagtcg gcgcgctgat tctgctgatc gcaggatttg ccattcttcg ccttttgttc
      661 agagcattaa ccagcacagc gtctgcgctg gcagggttca tattgctgtg tctgttcggt
      721 ccggctttac tggctggcta tatcactgaa cgcataaccc ggttattcca tattcgctgg
      781 ctggcaggcg tatttctgac gattgccgga atgatcatca gcttcatgtg gggacttgat
      841 ggtaaacata tcgcactgga ggctcatact tttgactctg taaaatttat tctgaccacc
      901 gctctcgccg ctggtctgct ggctcttccc gtgcagataa gaaccattca gcagaacggg
      961 ctcacacctg aagatatcag caaggaaatt aacgggtatt actgctgttt ttatactgct
     1021 tttttcctta tggcgtgttc tgcatacgca ccattgatcg cattgcagtt cgatatttca
     1081 ccctcactga tgtggtgggg cgggttgttg tactggctgg ctgcattagt gacgctgcta
     1141 tgggcggcca gccagatcca ggcgctgaaa agactgacca gtgccatcag ccagacactg
     1201 gaagaacaac cggtgctcaa cagtaaatcg tggctgagca gtttgcaaaa cgattacagc
     1261 cttcctgaaa cgctgacgga gcgcatctgg ctgacactca tttcacaacg gatttcccgg
     1321 ggagaactga gggaatttga actggcagac ggaaactggt tactgaacaa tgcctggtat
     1381 gaaagaaaca tggcagggtt taacgaacag ttgaaagaga acctgtcatt cacacctgat
     1441 gaactgaaaa cgctcttccg gaaccgcctg aatttatcac cggaagcgaa tgacgatttt
     1501 ctcgatcgtt gcctggacgg cggtgactgg tacccctttt cagaaggccg ccgttttgta
     1561 tcattccacc acgtggatga gcttcgtatc tgtgcctcct gcgggctgac agaagtacat
     1621 catgccccgg aaaatcataa gccggctccg gaatggtact gctcctctct ttgtcacgaa
     1681 acagaaacac tgtgtcagga catttatgaa cgttcttaca ccggttttat ttccgatgca
     1741 acggcgaatg gtctgattct catgaaactg ccggaaacct ggagtacaaa tgagaaaatg
     1801 tttgcttccg gagggcaggg acatgggttt gccgctgaac ggggaaacca tattgtcgac
     1861 agagtccgtc tgaaaaacgc acggatcctc ggtgataata atgccagaaa tggagcagac
     1921 agactggtca gcggaacaga aatccagacg aaatattgtt caactgcagc ccgtagcgtc
     1981 ggtgcggcat tcgacggaca aaacggacag tatcgttaca tgggaaatca tggtcccatg
     2041 caactggaag tcccccgtga tcagtatgcc ggcgctgtgg aaaccatgaa gaataagatc
     2101 cgcgaaggta aagtacccgg tgtaaccgat cccgcagaag cgtcccggct gattcgtcgg
     2161 ggacatctga cttataccca ggcccgtaat atcacccggt tcgggaccat cgaatcggtc
     2221 acttatgata ttgccgaggg gtcggttgtc agtctggcgg ccggagggat cagttttgcc
     2281 ctgacggcat cggtcttctg gctcagcacc ggcgatcgcg atgctgccct gcagacagct
     2341 gctgtccagg caggaaaaac cttcacccgc acactggctg tctacgtcac aacccagcaa
     2401 cttcaccggc tcactgttgt tcagggtatg ctgaagcata ttgatttttc gacggccagc
     2461 ccgactgtcc ggcaggcgct tcagaagggg accggtgcag gaaatatcag tgccctgaac
     2521 aaagtgatga agggtacgct ggtgacatct ctggcactgg tagctgtcac aaccagccct
     2581 gacatgatca aaatgttgcg gggacggatc tccggtgcgc agttcatcag gaatcttgcc
     2641 gtggcatctt ccggtgtggc aggtggtgct gtcgggtcag tggcgggcgg gatattgttc
     2701 agtccactgg gaccatttgg tgcactgaca gggcgtgtgg ttggcggtgt tctgggggga
     2761 atgattgcct ccgctgtatc aggaaaaatt gccggagcgc tggttgaaga agatcgcgtc
     2821 aaaattctgg caatgattca ggagcaggtg acatggcttg ccggcagttt cctgctgacc
     2881 ggacatgaga ttgaaaatct gaacgcgaat ctggcccgtg ttatcgatca gaatgctctg
     2941 gagatcattt tcgccgccgg tatacaacaa cgggccgcga ccaatatgtt aatcaaacca
     3001 ctggtggtca gtatcatcag gcaacgcccc gtcatggaat atgatgcatc ccatctcggc
     3061 aagatggtta accggctgga agaagcattc cccccggagc tcccggcata acgtgtgttc
     3121 tgctcctttt aggttttatc acctgatttg ccagtgtcat cgtcatgact cagagaggta
     3181 aataccatga ttcacctgtt caaaacctgc atgattaccg ccttcattct ggggttaacg
     3241 tggtctgccc cactccgggc acaggatcaa cgttacatca gtatacgcaa tacagatacg
     3301 atatggctcc cgggaaatat ttgtgcttac cagttccggc tggataatgg cggaaacggt
     3361 gaaggatttg gccccctcac catcactctg caactcaaag acaaatatgg tcagacgctg
     3421 gtgaccagaa aaatggaaac ggaagctttt ggtgacagta atgccacgcg aaccacagac
     3481 gcatttctgg aaacggagtg cgtggaaaat gtcgccacaa ccgaaatcat taaagcaact
     3541 gaagaaagta acggccatcg tgtcagtctg ccgttatcgg ttttcgatcc ccaggactac
     3601 catccactgc tgattaccgt ttccggaaaa aatgttaact gatccctgaa gtcagtttca
     3661 tgcttcagcc tctgccacaa cgtatggcag aggctattta tcaggagaaa ccagatatgc
     3721 cgggttacac acagagtatg tcctggctga aggatctttc tcatacgggc aggcggttgc
     3781 tgtcataacc gcttaccgca atgtgtttat ccaggatgac ccgggaatgc atttccgtcg
     3841 ggttatccgc aacgcggaag gtcagcgacg ctgacgcagc cggaactctg aagctgatgc
     3901 cggaaaacag cttaatgcgt ggctcgccag tggagggctt ctcagacaat aaacttcatt
     3961 gatttcatcc atcaggccgc gtcttctccg ggagacgcgg cttttttcat ttgttgcacc
     4021 agtaaatctt aaccaccgat aaggagcaaa gtatgcaatt agtcagtcgt tttggttatg
     4081 cgaaccagat acgccgtgac cgtccgctga cacatgaaga gctgatgcac catgtgccgg
     4141 ggattttcgg ggaagagaaa cacacgtccc gaagccagaa ctatacgtac atccccacca
     4201 tcaccgtact ggaaagtctg cagcgggaag gcttccagcc ctttttcgcc tgccagaccc
     4261 gtgtgcgcga cccgggccgc cggggataca caaaacacat gctgcgtctg cggcgggccg
     4321 gagagataaa cggagaacat gtccctgaaa ttattctgct caactcccat gacggtacct
     4381 ccagctacca gatgctgccg ggttacttca gattcgtctg ccagaacggg tgtgtctgtg
     4441 gtcagtctct gggggaagtg cgtgttccgc accggggaaa cgtggtggag aaagttattg
     4501 aaggggctta cgaagtggtg ggcgtgtttg accggattga ggagaa
//
LOCUS       DADLTY010000258         4537 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.262, whole genome shotgun sequence.
ACCESSION   DADLTY010000258 DADLTY010000000
VERSION     DADLTY010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.262"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(6..326)
                     /gene="psiF"
                     /locus_tag="JN334_004032"
     CDS             complement(6..326)
                     /gene="psiF"
                     /locus_tag="JN334_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HAY7439976.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(445..1860)
                     /gene="phoA"
                     /locus_tag="JN334_004033"
     CDS             complement(445..1860)
                     /gene="phoA"
                     /locus_tag="JN334_004033"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HAY7439977.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="JN334_004034"
     CDS             complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="JN334_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HAY7439978.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2403..2606)
                     /locus_tag="JN334_004035"
     CDS             complement(2403..2606)
                     /locus_tag="JN334_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439979.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2684..3778
                     /locus_tag="JN334_004036"
     CDS             2684..3778
                     /locus_tag="JN334_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HAY7439980.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3802..4014)
                     /locus_tag="JN334_004037"
     CDS             complement(3802..4014)
                     /locus_tag="JN334_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 family protein"
                     /protein_id="HAY7439981.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4274..>4537
                     /locus_tag="JN334_004038"
     CDS             4274..>4537
                     /locus_tag="JN334_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HAY7439982.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIF"
BASE COUNT         1104 a         1119 c         1067 g         1247 t
ORIGIN      
        1 aggtatcagg cggctttctt gaggcaggca ctcataaact tattacgatc atcacctttc
       61 agagcttgtt gcgttgcttg attattacat tcacgcatct tttgctgctg tggcgtcaaa
      121 cttttttcac caggcgcaga cttgctgttc ttcaggcaat cactcatgta ggtcttacga
      181 gcatcccctt tcaacgcctg cgccgtcgcc tgctgattac aggaggtcat acgctgttgt
      241 tgtggggtta aggttctctc ggcagcgccg acggtggtta aaaaaaccag accaaaaagc
      301 aaggtaacca gtaatgttat tttcatagca ccatccctct tcatgtttta accatgagcg
      361 tttgcgcccg tgatctgcca ttaagtctgg ttgctaacag caaaaaaacc acccggcagc
      421 gaaaattcac tgccgggcgc agttttattt cagccccaga gcggctttca tggtgtagaa
      481 gagatcggtc tggtcggtca gtccaacaac attggcggca tgcgggccat acgccgcaat
      541 acgcaactga ctgccggtat gttcttgtga atcctcttcg gaattcccgt aactcatcac
      601 catcactgcg ccatctttag tatttagcgc ctgagtgagg cccggcgctt tggtatccgg
      661 cgcaacaacc tggctggcgt gggcgtgatc agcggtgact atgaccagcg tgttaccctc
      721 ctttttagcg aattccagcg cccgttgtac ggcttcatcg agatcgaccg tctcgccaat
      781 ttgcccacaa gggttcgcag cgtgatcctg tttatcgatt gacgcacctt caacttgcag
      841 gaaaaatcct ttctcatttt tactcaacaa ttcaatggct ttgtcggtca tctgtgccag
      901 ggttggtaca ctgtcattac gttgcggatt aggcgtatag gtgactgcgg gcttatcgat
      961 attgccgtgg tacgttgctt tcggccctag ccagcgcact ggcatattgc cgtcagcaaa
     1021 caggccaagc aggggttttt gctgattcgc ttccgtcacc gaattcagtg aggcagcatc
     1081 gttcaccaac tgataaccac gcgcctgtgc ctgttcacgc agcgtttttc cctgccattc
     1141 accagcggtt gccgtttcag caaaggtttt tgcgccgccg ccaagcgtaa cgtcggcacg
     1201 agcgttaagc agctgttcgg taatcgatcc ttttccgcct ttttccagag cgttacccgg
     1261 acatttttca ctggtcgcgc tcggaccgta gcatttgcgc gaagtcacat gtgccaccag
     1321 cgcagcgggc gtggcatcct gcaactctgc ggtagaaacg ttaccggtcg ccagacctgc
     1381 ggcttttgcc atttccagaa tcgttgggtg atctttttcg tgaatatcga cgcccagcgc
     1441 gccgttatag gttttgacat cggttgacca ggcggttgct gatgcagccg agtcggtgac
     1501 gtagtccggt ttgccagttt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
     1561 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt
     1621 aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
     1681 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
     1741 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
     1801 ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac
     1861 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
     1921 ccgtgacaac tttatgacag ctgttgaaaa gattaacttt ttactgacaa ggatgcttca
     1981 ataacttctt tacgtaatcg cgcagcagct ctgtatcgtc gtcaggaatg ctggcatcgg
     2041 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg
     2101 ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt
     2161 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagcaatg agatttttca
     2221 tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag
     2281 tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgtttga
     2341 ttgccagcga aacgtttctc ttatttaaat aattacccac acaaaatata ggcttttaat
     2401 tatcaacctt tattataaat aaaagctatg aaatatattc tcttcgttaa cttataatta
     2461 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
     2521 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
     2581 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
     2641 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
     2701 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
     2761 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
     2821 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
     2881 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
     2941 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt
     3001 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
     3061 gtttgtaggt tctgatgttc tggcttcagc agcctgtatg gataaagatg tcaccaaacg
     3121 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
     3181 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
     3241 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
     3301 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
     3361 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
     3421 gatcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     3481 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
     3541 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     3601 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     3661 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt
     3721 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     3781 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     3841 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     3901 ccaacctttt gcttcaaatc ccagtacccc caccacgcca ttaagaatga atataaggcc
     3961 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     4021 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     4081 cattttcgta attattaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
     4141 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     4201 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     4261 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     4321 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     4381 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     4441 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     4501 cgggctgatc ccgtttttag caggctgcct cattttt
//
LOCUS       DADLTY010000259         4517 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.263, whole genome shotgun sequence.
ACCESSION   DADLTY010000259 DADLTY010000000
VERSION     DADLTY010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.263"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..1030)
                     /locus_tag="JN334_004039"
     CDS             complement(<1..1030)
                     /locus_tag="JN334_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7439983.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVE"
     gene            956..2122
                     /locus_tag="JN334_004040"
     CDS             956..2122
                     /locus_tag="JN334_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HAY7439984.1"
                     /translation="MLIPIGRPRERTGWIYSVIMINVSHIRTLSSIESTTSSPSIRLA
                     MESIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMT
                     VEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRT
                     QEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTP
                     SGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKS
                     RTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRIL
                     PGVSAMGQGAWHEPNMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            2183..4221
                     /locus_tag="JN334_004041"
                     /pseudo
     CDS             2183..4221
                     /locus_tag="JN334_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            4232..>4517
                     /locus_tag="JN334_004042"
     CDS             4232..>4517
                     /locus_tag="JN334_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit B"
                     /protein_id="HAY7439985.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVD"
BASE COUNT         1183 a         1139 c         1143 g         1052 t
ORIGIN      
        1 actcaacacg gaccagaagt ctctccagct cctcgtctgt gaacgctctg cgcttggagt
       61 cgatatcctt ggtcagcttc atccgttcaa tagggttgcg gccctcaagt tgcccattca
      121 tcaggccata atcgagacac atcttcgctt tggtcagcag cttgttgatt gtcgatggtc
      181 tcacctcaga agccagcata gagtccctga cagccagcca cccagagcgg gacatcaggt
      241 ccatgtcttc aggcaggaac cgggagacag tattgagtga ggacgagagg tccttgtagc
      301 tggatgtctt cagggtctgg accttctcag ccagcagcga ggacactagg cttgaaaaaa
      361 ctacagatga ctggcgctcc tgtgtgaaaa ctgccggtct atccccctgc tcattattca
      421 gaattgacgt agagtcgcct atagttgagt catcagtaag gttattatca tcaatcagct
      481 taatcagacc agaggtatca ccagtgtcga ctctctgctg tgctgcggtc aggacttcca
      541 gcgctaacct tatgcccttc tgctggtcca gactcaaggc ttgcgtagcg gctagctcct
      601 tcaggttgga cttctcgtcg accagagtag cgacctcaag tccattccag taatcgtcac
      661 tagcgtctgt caggaactgc tcagccatag accggagatg ctctgtaagc tcctgtagtg
      721 agacttcagg cctatccaac atgaaggcct tagcggtggc tgcaagctct ctctgacgca
      781 ttgtggctac tctcctgtct gtgctgcggg tcgatactga aagaaacggt gtggtctgtc
      841 ccttgagtct gaagcggata gtatatcgac cattggctct gcggactaac tgaggcttag
      901 ctacgctgag tagattgatt tgtgtcagcc ttgtgtcagg gcttgaggtg tagacttgct
      961 gataccgata gggagaccca gagaaagaac tggatggata tacagtgtta tcatgattaa
     1021 tgtcagtcat atcaggacct taagtagcat tgagtcaact acttcaagtc catccatccg
     1081 tctggcaatg gagtccatct ggaactacgc gggaaatacc ctcgttaatc agcattctga
     1141 catcaacaaa acgcatgaaa ttctgcagga cgaatcgaaa tgcgaaatga ttgtggtcat
     1201 cgaaaacttt atgacctcat cggcaaaata tgccgacatt ctgctggaag acctgatgac
     1261 cgttgagcag gaagatatta ttcctaacga ctacgccggt aacatgggat atctcatttt
     1321 cctccagcca gtcaccagcg aaaaattcga acgtaaaccg atttactgga tcctgagtga
     1381 agtcgcgaaa cgtcttggac ctgacgtcta tcaaaagttc acagaaggtc gcacgcagga
     1441 acaatggtta caacatctgt acgccaaaat gcttgccaaa gatccggcgt taccgtctta
     1501 cgacgaactg aaaaaaatgg gtatctataa gcgtaaagat cccaatggcc attttgtcgc
     1561 ctacaaagca tttcgtgacg accccgaggc aaatccactt aaaacgcctt ccggtaagat
     1621 tgaaatttat tccagcaagc tggcggaaat tgcccgtacc tgggaactgg aaaaagatga
     1681 agtgataagc ccactgccag tttatgcctc aacctttgaa ggctgggact cccctgaacg
     1741 tagaaccttc ccactgcaac tgtttggttt ccattacaaa tcccgtactc actcgaccta
     1801 cggcaacatt gatgtcctga aggcagcctg ccgtcaggag gtgtggatca accctataga
     1861 tgcacagaaa cgtgggattg ccaacggcga tatggtgcgg gtgtttaacc atcgtggcga
     1921 agttcggcta ccagccaaag taacaccacg cattctccct ggagttagcg ctatgggcca
     1981 gggagcctgg cacgagccca atatgtctgg cgataaaatc gaccatggcg gatgtgtgaa
     2041 tacgctaacc actctgcgcc cttcaccact ggcgaaggga aacccgcagc acactaatct
     2101 ggtcgagatc gaaaaaatat aacccactac aaccataaat tctggcatga cattttgttt
     2161 gaaaagcaat aagtgagtaa tgatgaaaat ccataccaca gaggcgctca tgaaggctga
     2221 aatcagccat agaagtctga tgaaaacctc cgcacttggc agtctcgcgc tggcaagcag
     2281 tgctttcact ctgccatttt cccaaatggt ccgggcggca gaggctccgg tagaaaagaa
     2341 aacggtctgg agttcctgca ccgttaactg cgggagccgc tgtctgctac gtttgcatgt
     2401 gaaagatgac accgtgtact gggtggagtc tgatacgaca ggtgacgacg tttacggtaa
     2461 tcatcaggtt cgggcatgtt tacgcgggcg ctctatccgc cgacggatga atcatcctga
     2521 caggttgaaa tatcccatga agcgcgtcgg cgagcgcggt gaaggtaaat ttgaacggat
     2581 aagttgggac gaagccctgg ataccatcag tgataatctt cggcggatcc tgaaagatta
     2641 cggcaatgag gctgtacatg tcctgtacgg aacaggcgta gatggcggaa atatcaccaa
     2701 ctcgaacgtc ccgtaccgtc tgatgaactc ttgcgggggt tttctcagtc gctatggcag
     2761 ctacagtacc gcacagatca gtgccgcaat gagttatatg ttcggtgcca atgatggcaa
     2821 cagcccggat gatatcgcca atacgaaact ggtcgttatg ttcggaaata acccggcaga
     2881 aacgcggatg agcggcggtg gtgtcactta ctacgtcgag caagcccgcg aacgttcaaa
     2941 cgcacgcatg atcgtcatcg atccacgcta taacgacaca gctgccgggc gtgaagatga
     3001 atggctgccc attcgccctg gcaccgatgg cgcactggcc tgtgcgattg cctgggtact
     3061 gattactgaa aacatggtcg atcagccatt tctcgacaaa tattgtgttg gttacgatga
     3121 aaaaacgctg cccgccaacg caccacgtaa cgcgcattat aaagcctata ttctgggcga
     3181 agggcctgac ggcatagcta aaacgccgga atgggcagca aaaatcacca gcatcccggc
     3241 agaaaaaatt atccagttgg cacgagagat cggttcagca aaacctgctt atatttgtca
     3301 gggttggggg ccacaacgac actccaacgg cgaacaaacg tcccgcgcta ttgccatgct
     3361 ttccgttctt accggtaacg tcggcataaa cggcggcaac tcaggtgtac gcgaaggtag
     3421 ctgggatctg ggggtagaat ggttcccgat gctcgagaat cctgttaaaa cgcagatttc
     3481 cgtttttaca tggacagatg ccatcgacca tggtacgaaa atgaccgcga cccgtgatgg
     3541 tgttcgtgga aaagaaaaac tggatgtccc catcaagttt ttatggtgct acgccagtaa
     3601 cacattgatc aatcaacatg gcgacatcaa tcacacccat gaggtgcttc aggatgacag
     3661 caagtgcgag atgattgttg gcattgacca cttcatgacg gcctcggcta agtattgcga
     3721 tatcctgttg cctgacctga tgccaacaga gcaagaagac cttatctctc atgaatctgc
     3781 agggaatatg ggctatgtga tcctcgccca acccgcaacc tcagcaaaat ttgaacgcaa
     3841 acccatctac tggatgctga gtgaagtcgc caaaccaaag cgcgtaccca ctccagctac
     3901 ggcaatattg atgtgttaca gcaggcctgc ccacaagagg tgtggatcaa ccccattgat
     3961 gctcaggcac gcggtatccg tcatggcgat accgtgcggg tatttaacaa taatggagaa
     4021 atgctgattg ccgcaaaagt gactccgcgt attctgcctg gcgttaccgc catcgggcaa
     4081 ggtgcgtggc ttaaagcgga tatgtttggt gaccgggtcg atcacggcgg cagtatcaat
     4141 attctgacct ctcaccgccc ttcaccgctg gcaaagggaa acccgtcgca cagcaatctt
     4201 gtccagatcg aaaaggttta aggagtagcc catgaccaca caatatggat tttttatcga
     4261 ttccagccgc tgtactggct gtaaaacctg cgaactggcg tgcaaagatt ttaaagatct
     4321 tggcccggaa gtcagtttcc gccgtattta tgaatatgct ggcggcgact ggcaggaaga
     4381 taatggcatc tggcaccaga acgtgttcgc ttattatctc tccatctcct gtaaccattg
     4441 cgatgacccc gcatgtacaa aagtctgccc cagcggcgca atgcataagc gtgaagatgg
     4501 ctttgtggtg gttgatg
//
LOCUS       DADLTY010000260         4493 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.264, whole genome shotgun sequence.
ACCESSION   DADLTY010000260 DADLTY010000000
VERSION     DADLTY010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.264"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(217..292)
                     /locus_tag="JN334_004043"
     tRNA            complement(217..292)
                     /locus_tag="JN334_004043"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(257..259),aa:Ile,seq:cat)
     gene            851..3103
                     /locus_tag="JN334_004044"
     CDS             851..3103
                     /locus_tag="JN334_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7439986.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSNQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3439..4371
                     /gene="csiD"
                     /locus_tag="JN334_004045"
     CDS             3439..4371
                     /gene="csiD"
                     /locus_tag="JN334_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HAY7439987.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4391..>4493
                     /locus_tag="JN334_004046"
     CDS             4391..>4493
                     /locus_tag="JN334_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7439988.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEK"
BASE COUNT         1390 a          966 c          876 g         1261 t
ORIGIN      
        1 tttgcccgct tggcatagcg ggcatttttt ccaggtactt ttgaatgagt actgatgaat
       61 aaatacattg cagtggcgtg ccacgtacca aaacaccagc cctcattcga aaccacccac
      121 cgcacttctt ccttgaaatg gcgttagtca tgaaatatag accgccatcg agtacccctt
      181 gtacccttaa ctcttcctga tacatgaatg atgatttggt ggcccttgct ggacttgaac
      241 cagcgaccaa gcgattatga gtcgcctgct ctaaccactg agctaaaggg ccttaagtat
      301 gcaataacaa tacttataaa ccacgcaata aacatgatga tcttatgatg taaataacag
      361 atttttatgc gttcccattc accttgggtc gtgattacac gcgacataaa acccgacacc
      421 gcctccattc gcaaagtcga tactcgcagt caacaagcaa atgttaataa ttagcactat
      481 ctatagttat catcgattca atgatacttt gtaatgattt tgtatctaat aatataactt
      541 tattacatta gctgaagagt tttcgcatca ttatgatatc tgttactttt cactccataa
      601 aaataaactt catatagcaa tatattcttt catagatctt attctgctaa tcattagttt
      661 cgtatgcgcg atttttgaca gttgcctctc cagaccacat cgataattaa aaaacagatt
      721 taaacattat ccttttccat atagatattg gataaaaata ggacatctgt ttgcaattac
      781 tttcacaaca attaaacatt tttatgtttt cgtatacatc atattactct accattagag
      841 gaacgttatt atgttttcta taaaaccagg acctggaaat ttacctatcg acaaccccac
      901 attgttatca tggaacatta ctgatgggga tctaaattcc aaatttaata cattagaata
      961 tctaaactgt ataacaaata ttattaatgc ttgtggagtt tatcctcagg atttaaaaga
     1021 cagagaaatt atatcaactt ttcacgcaga aaaagtcatt aatgatctgt taaaaaacga
     1081 ttataaaatt tccctttctc cagatacaac ttaccgagag ctgaataaag ctgcacagag
     1141 tagcattaca gcgccagaca ggataggaga agggaaaaca tgggtttatc aacgagatac
     1201 aatggttgaa agaggtgata acagtggtgt tcatcagtat ggtccagctg aacatttcac
     1261 tcacattata tctgacaaac cgtccccaaa agataagtat gttgcatatg ctattaacat
     1321 tcctgactat gagctagcag ccgatgtata taatattaac gtgacgtcac cttccggaca
     1381 gcaagaaaca tttaaaatac taatcaatcc agaacatcta cggcaaacac ttgagcgtaa
     1441 atctcttact gctgttcaga aatcacaatg tgaaatcatc acccccaaaa aacctggcga
     1501 agcgattctt catgctttta atgccaccta ccagcaaatc agggaaaata tgtctgagtt
     1561 tgcacgttcc cattatgggt atatacaaat ccctccagtg acaactttcc gcgccgacgg
     1621 accagaaact cccgaagaag aaaaaggtta ctggtttcac gcttatcaac ccgaagatct
     1681 ttgtaccatc cataatccaa tgggggattt gcaggatttt atcgcattgg ttaaagatgc
     1741 taaaaaattt ggtatcgata tcattcctga ttataccttt aactttatgg gaatcggggg
     1801 aagtggtaaa aatgacctgg attatccctc tgctgatata cgagcgaaga tcagtaaaga
     1861 tatagaaagt ggtatccctg gctattggca aggtcaggtt ttgattccat tcattaaaga
     1921 tccagtaaca aaagaacgta aacaaatcca tccagaagat atacatctca ctgcaaaaga
     1981 cttcgaagca agtaaagata acatctctaa ggatgaatgg aaaaacctcc atgcattaaa
     2041 agaaaagagt ttaaatggaa tgcctaaaac aacccccaaa agtaaccagg ttattatgtt
     2101 gcaaaatcaa tacgttcgtg aaatgcgaaa atatggcgta cgaggtttac gttatgatgc
     2161 agcaaaacac tcaaaacatg aacaaataga aagatcaata accccaccgc ttaaaaatta
     2221 taatgagcga ttacacaata ctaacttatt taacccaaaa tatcatgaaa aaaccgttat
     2281 gaattacatg gaatatctgg taacttgtca gttgaatgaa gaacaaatgt catcgctgct
     2341 ttatgaaaga gatgatttaa gcgccattga tttttcattg ctcatgaaga cgataaaagc
     2401 cttttcattc gggggagatc tcctaaccct tgcatcaaaa ccgggttcca caatctcaag
     2461 catcccatca gaaagacgga tattgattaa cattaaccac gattttccta acaatggcaa
     2521 tcttttcaat gactttctat ttaaccatca acaagatgaa caattagcaa tggcatatat
     2581 ggccgctctc ccgttcagca ggcctttagt ttactgggat ggccaggtat taaaatcaac
     2641 gactgaaatt aaaaattatg atgggcccac gcgtgtcggt ggtgaggcgt ggcttaataa
     2701 aggttgctct acctatcagc agctctacaa tgaattccac gcattatata tagataaagc
     2761 aggaatatgg agcgcatttg agggtgtatt tgcaactaaa aacgttctgg cctttagtcg
     2821 tggggattct gtgaacatta atcactctcc tcatgatgga ctagttataa taaataaagg
     2881 aaacgaagaa gttgaaggta cctggcctaa caaattgcaa cctggaatat ataaaaacat
     2941 ggggagtaat agcgttaaca ttattattaa taatacccga aaaattatcc ccccttgtaa
     3001 agtatttacg cttagaggcg gatctctaaa tatcaatatt cctaggcgta gcgctcttct
     3061 tttagggaaa acaggagaac cgccgaacta tctctattta taatttaatg ttatatctgc
     3121 cccgataaaa cggggcagat aatatgttta gtttactaac ggtcattttg tagtgaagcc
     3181 atttactgtt ttttatcgac cagataatct gttctctaat gttaactccc cctaacctgt
     3241 tgctttagtt attcatttcc tgtctcactt tgccttaata ccctacgtta aatgttacta
     3301 atttgttgct tttgatcaca ataagaaagc aatatgtcgc ttttgtgcgc atttttcaga
     3361 aatgtagata tttttagatt atggctacga aatgagcatc gccatgtcac cctacatctc
     3421 ataagaggat cgcttctgat gaatgcactg accgccgtac aaaataacgc tgtcgattca
     3481 ggccaggact atagcggatt cactctcatc ccgtcggcgc aatccccgcg tctgctggaa
     3541 ctcaccttca ccgaacagac gaccaaacag tttctcgaac aggttgccga atggcccgtg
     3601 caggcgctgg agtacaaatc gtttctgcgt tttcgggtag gcaaaattct tgacgatctg
     3661 tgtgcgaatc agctgcaacc attgctgttg aagaccctgc taaaccgtgc tgaaggtgcg
     3721 ctgttgatca atgcggtagg tatcgatgat gtggcgcagg cagatgagat ggtgaagctg
     3781 gcaacggcgg tagcgcatct gattggtcgc tcaaatttcg atgcgatgag cggtcagtat
     3841 tacgcgcgtt tcgtggtgaa aaatgtcgat aactcagaca gctatctgcg tcagccgcac
     3901 cgcgtgatgg aactgcacaa cgacggcacg tatgtcgaag agatcaccga ttacgtgctg
     3961 atgatgaaaa tcgatgactg ggaacatctg gaccactatt tccgccatcc actggcgcgt
     4021 cgcccgatgc gctttgccgc gccgccgagc aaaaacgtca gcaaagatgt tttccatcca
     4081 gtgttcgacg ttgatcaaca gggtcgcccg gtgatgcgct atatcgacca gttcgtccag
     4141 ccaaaagatt ttgaagaagg cgtgtggttg agcgagcttt ccgatgccat tgaaaccagc
     4201 aaaggcattc tttctgtacc cgttcccgtt ggcaaattcc tgttgataaa caacctgttc
     4261 tggctgcacg gtcgcgaccg ctttactccg cacccggatc tgcgccgtga actgatgcgt
     4321 cagcgtggct atttcgctta cgccactcac cactaccaga cgcatcagta agcgcaaagg
     4381 aattgagcgg atgtatgatt ttgtgattat tggcggcggc atcatcggca tgtcgaccgc
     4441 catgcaactg attgatgttt atccggatgc ccgcattgcg ttgctggaaa aag
//
LOCUS       DADLTY010000261         4347 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.265, whole genome shotgun sequence.
ACCESSION   DADLTY010000261 DADLTY010000000
VERSION     DADLTY010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.265"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..2602
                     /locus_tag="JN334_004047"
     CDS             <1..2602
                     /locus_tag="JN334_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY7439989.1"
                     /translation="ELRRLAKEDIHFFDLKLLAFKTRGNRAATRHFGTKALVANQYDA
                     IDVTEFIKHMRLNESIPLSRYLPWSSHLHEQVVKNKKGDFVSTWEIGGIIFECEDEHY
                     LATLDMQLNNVIRSFEGLPVTFYTHLVSDEFTDTLECESGIPFSDEIARLFYASREGK
                     PFYQRRLFFTVCFNPHTGIERATMKQKSLGERKTLLDNALQVMQEHRQAIGTALSRYA
                     ARPLGTYEENGHVFSSQLAFYNFLITGRWQKVRVTRTPFYDVLGGTDIFPSTDIGQVS
                     HTDGQSYFRGLEIKDYSTETITGLYDSLLYARCRYVFTQSYTCMGKDEARGCIRTIEK
                     RLKSAEDDAISQQEDLKVARDMLQSGIISFGKYHQTLIVSAPTSEQVIRDTNTLADTL
                     KDLGFIMTLSRESLSAAYLAQLPGVYHLRPRLVPVSSLNYAEMVSFHNFHPHKRDGNA
                     WGEAIAVVPTPSGGCQYVNLHNSQADVDEFSEKPPGNTMLLGSTGTGKSTLLMMFQHL
                     MQKYRRPESFAPGATKKRFTSVVFDKDRSAEMSIRLQGGSYFRFRSGEKTGLNPFALA
                     PTKRNRNFLKKLMRMMCTRDGKPLDPRDEERLSEAVDTIMLDYPPELRRNGITRMLEV
                     LHEAPTKDARMNGLRIRLQQWAQGGEFGWVFDNDVDTFDIDNIDNFGIDGTEFLNDKD
                     VRSPLTFYLLYRVTSLLDGRRLVIFMDEFWKWLQDPEFSKFALDMLKVIRKLNGIFFP
                     ATQSPEEMCKHEISAAIIEQCSTRIFMANQQAKEYDYVDMLRVPKAVYDIVTTLDPYA
                     RQMVIMKSPLRRGDVRPYIAQITLDLSGLGRYTKLLSASADNLKIFDEIWQEGMKPED
                     WKDEMLRRAV"
     gene            2627..3337
                     /locus_tag="JN334_004048"
     CDS             2627..3337
                     /locus_tag="JN334_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015696806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB5"
                     /protein_id="HAY7439990.1"
                     /translation="MKTTLTALTVALCLFHVPAQAGIPVFDEVANASNIQQWAEKLQQ
                     WQQTVQHYQSQMNAYKQQLATATGIRDVQAFLNQAKNLTADIKNLKNRGISLNDLLTG
                     SGGSYAGELNRLYDKYKLFDNCPENASEAYRDSCKQMLLNQAVSVEDTSDIQEKISDT
                     LSDISNLASRIENAKDSKESQDLANVVSARSIQLNALTTQWEMSVKQSEMRNQLLAEQ
                     RRKAQAEQMRTAPIADLN"
     gene            3349..3633
                     /locus_tag="JN334_004049"
     CDS             3349..3633
                     /locus_tag="JN334_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HAY7439991.1"
                     /translation="MKLKTLTFIVLASVAALTGCEDKTSVDWYMAHHDDMLAKYRECI
                     FNHNFVPTDCQNARSALHREKAKPDVAEGYKQILKDLDKARVQEPIADLN"
     gene            3651..>4347
                     /locus_tag="JN334_004050"
     CDS             3651..>4347
                     /locus_tag="JN334_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HAY7439992.1"
                     /translation="MSGIFVGLNSHIMEGFDSVLTSVAADTGGWMAHLFSIAVLSLYI
                     TWRAYQTMAGKLVRPFEEIVWDVSRMLIIMIFVTNAGGYLDLVSDAITGLRDGFSGDA
                     SIWARLDTVWEKAQALGEKLYSYDDSTYVALKGTLAQFLVWAGVMLLVGITAIINLIA
                     ELVMLLMLATAPIFIYCLMWSWFRDMFSNWLRIILGCILTSLFSGIALTVVMDYLDYI
                     LAGATKASDANYLT"
BASE COUNT         1019 a         1227 c         1198 g          903 t
ORIGIN      
        1 cgagctgaga cggctggcaa aagaggacat tcatttcttt gacctgaagt tgctggcgtt
       61 taaaacgcgt ggcaaccgcg cggcgacccg gcatttcggg acaaaggcgc ttgtcgcaaa
      121 ccagtacgac gccatagacg ttacggagtt tataaaacat atgcggctca acgagagtat
      181 cccgctgtcc cgctatctcc cctggtcttc ccacctgcac gagcaggtgg tgaagaacaa
      241 aaaaggtgac tttgtcagca cctgggagat cggcggtatc atttttgaat gcgaggatga
      301 acattacctc gccaccctgg acatgcagct caacaacgtt atccgctcct ttgaggggct
      361 acccgttacc ttctataccc atctggtgag cgacgaattt accgacacgc ttgaatgtga
      421 atccgggatt ccgttttcag atgaaatcgc gcgtctgttt tacgcctccc gcgagggcaa
      481 acctttctac cagcgtcgcc tcttcttcac cgtctgcttc aatccgcaca ccggcattga
      541 gcgggcgacc atgaaacaga agtccctggg cgaacgtaaa accctgctcg acaatgccct
      601 gcaggtgatg caggagcacc ggcaagcgat tggcacggcg ctgtcacgct acgcggcgcg
      661 gccgcttggc acttatgagg agaacgggca cgtgttctcc tcccagctgg cgttttacaa
      721 ctttctgatc accggcaggt ggcagaaagt ccgcgtcacc cgcacgccgt tttatgacgt
      781 gctgggcggc acggatattt tcccttccac cgacattggc caggtcagcc acaccgacgg
      841 ccagagctac tttcgcgggc tggaaatcaa ggattacagc actgaaacca tcaccgggct
      901 gtatgactcg cttctttatg cccgctgccg gtacgtgttc acgcagtcct atacctgcat
      961 gggtaaggat gaagcgcggg gctgcatccg caccatcgag aaacgcctga agtctgccga
     1021 ggatgatgcg atctcccagc aggaagacct gaaggtcgcc cgcgacatgc ttcagtccgg
     1081 cattatttca ttcgggaaat atcaccagac gcttattgtc agcgccccaa ccagcgagca
     1141 ggttatccgg gacaccaaca ccctggcgga taccctgaag gatctcggtt tcatcatgac
     1201 cctgtcccgc gagtcgctgt cggcggcgta tctcgcgcag ttgccgggtg tgtatcacct
     1261 gcgcccgcgt ctggtgccgg tgagcagcct caactacgcc gaaatggtgt cgtttcacaa
     1321 ctttcacccg cacaagcggg acgggaatgc ctggggggag gcgattgccg ttgttccgac
     1381 cccgtccggg ggatgtcagt acgtgaatct gcacaacagc caggccgatg ttgacgagtt
     1441 cagcgagaag ccgccgggta acaccatgct gctcggcagt accgggacgg ggaaatcgac
     1501 cctgctgatg atgttccagc atctgatgca gaaataccgg cgtcctgagt cgtttgcgcc
     1561 gggggcgacg aaaaagcgct tcacctcggt ggtctttgat aaagaccgtt cggcggaaat
     1621 gtcgatacgc ctccagggcg gctcgtactt ccgtttccgt tcaggcgaga agaccggcct
     1681 taacccgttt gcgctcgctc caacgaaacg taaccgcaac ttcctcaaga agctgatgcg
     1741 aatgatgtgc acaagggacg gcaaaccgct cgacccgcgc gatgaagaac gcctcagcga
     1801 ggcagtggac accatcatgc tggactaccc gcctgagctt cgtcgcaacg gcatcacccg
     1861 catgctggaa gtgctgcatg aagccccgac gaaggacgca cggatgaacg ggctgcgcat
     1921 ccgtctccag cagtgggcgc agggcgggga gtttggctgg gtgtttgata atgacgtgga
     1981 cacctttgat atcgacaata ttgataattt cggcatcgac ggcacggagt tcctgaacga
     2041 caaagacgtg cgcagcccgc tcaccttcta cctgctctac cgcgtgacca gcctcctcga
     2101 tggccgccgc ctggtcattt tcatggatga attctggaaa tggcttcagg acccggagtt
     2161 ttcaaaattc gccctcgaca tgctgaaggt cattcgaaag ctcaacggca ttttcttccc
     2221 ggccacgcag tcgccggagg agatgtgcaa gcacgagatc tcggcggcca ttatcgagca
     2281 gtgtagtacc cgcatcttca tggcgaacca gcaggcgaag gagtacgact acgtggacat
     2341 gctgcgggtg ccgaaagcgg tttacgacat cgtgaccacc ctcgacccct acgcccgcca
     2401 gatggtgatc atgaaatctc cgctgcggcg tggtgacgtg cgcccgtata tcgcacaaat
     2461 caccctcgac ctctccgggc taggtcgcta cacgaagctg ctcagcgcca gtgcggacaa
     2521 cctgaaaatt tttgacgaga tctggcagga gggtatgaag ccggaagact ggaaagacga
     2581 aatgctgaga cgtgccgttt aatcacaaaa ggataacacc atgaaactga aaaccaccct
     2641 gacggcgctg accgtcgcct tgtgtctctt tcacgtcccg gctcaggccg gtattcccgt
     2701 ttttgatgaa gtagccaacg ccagcaacat ccagcagtgg gcggagaagc tccagcagtg
     2761 gcagcagacg gttcaacatt atcagtcgca gatgaacgct tataaacaac agctggcaac
     2821 ggccaccggt attcgggacg tccaggcatt cctgaatcag gcaaaaaacc tgacggctga
     2881 cattaaaaat ctgaaaaaca gggggatttc cttaaacgat ctgttaaccg gctcaggcgg
     2941 gtcttatgct ggggaactaa acaggctgta tgacaaatac aaactgttcg acaactgccc
     3001 ggagaacgcc agcgaggcgt accgggatag ctgcaaacag atgctcctca atcaggcggt
     3061 gtccgtcgag gacacttctg acatccagga aaaaatttcc gacacgctga gcgatattag
     3121 taatctcgcc agtcgcatcg agaatgcgaa ggacagtaaa gaatcgcagg atctggcgaa
     3181 cgtagtgtcc gcgcggagta tccagctgaa cgccctgacc actcaatggg aaatgtcggt
     3241 caaacaaagt gagatgcgca accagctgct tgccgaacaa cgccgaaagg cgcaggccga
     3301 gcaaatgcgc acagctccaa tcgcagacct taactaacag gtgaccccat gaaactgaag
     3361 acactgacct ttattgttct ggcctcagtg gccgcgctta ctggctgcga ggataaaacc
     3421 tccgtagact ggtatatggc acaccatgac gacatgctgg cgaaatacag ggagtgcatt
     3481 tttaatcata attttgtgcc caccgattgc cagaatgcac gctccgccct tcaccgtgaa
     3541 aaagctaaac ctgatgttgc cgaaggatat aagcaaatcc tgaaagacct tgataaagct
     3601 cgcgtgcaag agcctattgc tgacctgaat tagcgacagg agaacgcacc atgagtggaa
     3661 tattcgtcgg attaaacagc cacatcatgg aggggtttga cagcgtgctc accagcgtgg
     3721 ccgccgatac gggggggtgg atggcacacc tgttctccat cgcggtgctc tcgctgtaca
     3781 tcacgtggcg cgcctaccag acgatggcag gcaagctagt ccgccccttc gaggagatcg
     3841 tgtgggacgt gagccggatg ctcatcatca tgattttcgt caccaacgcc ggaggctatc
     3901 tcgatctggt ttcggatgcc atcaccggcc tgcgggacgg attcagcggc gacgcgtcga
     3961 tctgggcgcg gctggatacc gtctgggaaa aggctcaagc acttggggag aagctttact
     4021 cttatgatga ttcaacctat gtcgctctaa aaggcactct ggcgcagttt ctggtctggg
     4081 caggcgtcat gttactggta ggcatcacgg ccatcattaa cctgatagcc gaactggtga
     4141 tgctgctgat gctggcgacc gccccgatct ttatctactg cctgatgtgg tcgtggttcc
     4201 gggatatgtt cagcaactgg ctcagaatta ttctgggctg catccttaca tccctgttca
     4261 gcggcatcgc cctgacggtg gtaatggatt acctggacta catcctggcg ggcgccacaa
     4321 aagcatccga tgccaactac ctgactc
//
LOCUS       DADLTY010000262         4318 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.266, whole genome shotgun sequence.
ACCESSION   DADLTY010000262 DADLTY010000000
VERSION     DADLTY010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.266"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..1173)
                     /locus_tag="JN334_004051"
     CDS             complement(<1..1173)
                     /locus_tag="JN334_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY7439993.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1372..1456)
                     /locus_tag="JN334_004052"
     tRNA            complement(1372..1456)
                     /locus_tag="JN334_004052"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Leu,seq:caa)
     gene            1652..2671
                     /gene="ahr"
                     /locus_tag="JN334_004053"
     CDS             1652..2671
                     /gene="ahr"
                     /locus_tag="JN334_004053"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HAY7439994.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2800..4302
                     /locus_tag="JN334_004054"
     CDS             2800..4302
                     /locus_tag="JN334_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HAY7439995.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
BASE COUNT         1030 a         1090 c         1183 g         1015 t
ORIGIN      
        1 caacctacgc tccccaagat gttccgcctt atggatgtaa gctgctcgca cagagctacg
       61 ctcctggtga ctcatctgcc gctctaccgc atccctcgac cataatcccg actcaatcaa
      121 cgaactacaa gccatcgtcc tgaagccatg cccacaaacc tcgaccttcg tgtcataccc
      181 cattacgcgc aaagccttgt tcaccgtatt ctcactcatc ggcttacgtg gatcgtgatc
      241 gcccacaaaa atcagctctc gatttccgct catgcttttg atcttttcca aaatacttag
      301 ggcctgccgc gacaaaggta caaggtgtgg tgtccgcatc ttcgagccac gctgagaatg
      361 cttgactcct tccagttgct cacgctcacc tgggattgtc cacatagccg tttcaaaatc
      421 tatttctgac cagcgggcaa aacgcagctc gctcgaacgg atgaaaacca ataaagtgag
      481 ttcaaccgcc agtcgagtta acggtctgcc agaatagtga tctatgcgat gaagtaattc
      541 aggtatacgg ttcagttcaa gagctgcacg gtgctgtctt ttcgccgtag caaccgcacc
      601 tgcaatctct tgcgcggggt tgtagtcgat taagccgctc tgaacggcaa agcgcataat
      661 cgcggtagta cgctgctgta aacgggcggc aacttcgagc cgcccggatg actcgactgc
      721 tttaatgggt acaagaagat cccgcgtctt cagttccgca atgttccgct taccgatagc
      781 agtaaagaga ttatcctcca gacttttcaa aacacgagcg ctatgcgatg ccgaccactt
      841 ctgattgctg gcgtgccagt ctctggcgac tacttcaaac gttatcgcct cttgttcctg
      901 ttctacctta acagctttct tgttttcact gggatcgaca ccattagcta aaagcttacg
      961 agcctcatcc cggcgtgccc tggcatccgc caacgacact tcggggtatt tccccaatgc
     1021 cagcatcttc tctttgccgc caaagcgata acgtagccgc cagtatttgg agccgttagg
     1081 gtgaaccagc aaaaccatgc cttcaccgtc ggtcagctta taggcttttg cttcaggctt
     1141 agccgaacga accttcacat cactcagagc catgatgagt atcctttcaa gggttctgtg
     1201 tgggtacaaa cattatcgaa ccgggaaata cccgcagttg tacccgcatc agtaagttga
     1261 tgtagattga atcaggttga cttaggttga gtgaaaaagc gaggaaagcc ttgcggatac
     1321 tggattttag gcacaaaaaa agacgtccgt tgacgtctat tgatgttccg atggtgccga
     1381 aggccggact cgaaccggca cgtatttcta cggttgattt tgaatcaact gcgtctaccg
     1441 atttcgccac ttcggcactg aagaggtatg cggaaaacgt tgtggattat acctgtcgca
     1501 cgccaccatg caagcgccag cttgcaccca cctcgctaag tgctgaaatt ttcagcatta
     1561 cactcagcga tatgtaacct ttgtcacact ccaggcaccc cgccctgcca tgctctacac
     1621 ttcccaaaca acaccagaga aggccccaaa aatgtcgatg ataaaaagct atgccgcaaa
     1681 agaagcgggc ggcgaactgg aagtttatga gtacgatccc ggtgagctga agccacaaga
     1741 tgttgaagtg caggtggatt actgcgggat ttgtcattcc gatctgtcga tgatcgataa
     1801 cgaatgggga ttttcacaat atccgctggt tgccgggcat gaggtgattg ggcgcgtagt
     1861 ggcactcggg agcgccgcgc aggataaagg tttgcaggtc ggtcagcgtg tcgggattgg
     1921 ctggacggcg cgtagctgtg gtcactgcga cgcctgtatt agcggtaatc agatcaactg
     1981 cgagcaaggt gcggtgccga caattatgaa tcgcggtggc tttgccgaga agttgcgtgc
     2041 ggactggcaa tgggtgattc cactgccaga aaatattgat atcgagtccg ccgggccgct
     2101 gttgtgcggc ggtatcacgg tctttaaacc actgttgatg caccatatca ctgctaccag
     2161 ccgcgttggg gtaattggta ttggcgggct ggggcatatc gctataaaac ttctgcacgc
     2221 aatgggatgc gaggtgacgg cctttagttc taatccggcg aaagagcagg aagtactggc
     2281 gatgggtgcc gataaagtgg tgaatagccg cgatccgcag gcactgaaag ccctgtcggg
     2341 gcagtttgat ctcattatca atactgtgaa cgtcagcctc gactggcagc cttattttga
     2401 ggcgctgacc tatggcggta atttccatac ggtcggtgcg gttctcacgc cgctgtctgt
     2461 tccggccttt acgttaattg cgggcgaccg cagcatctct ggttctgcta ccggcacgcc
     2521 ttatgagctg cgaaagctga tgcgctttgc cgcccgcagc aaggttgcgc cgacaaccga
     2581 actgttcccg atgtcgaaaa ttaacgacgc cattcagcat gtacgcgatg gtaaagcgcg
     2641 ctaccgagta gtcctgaaag ccgacttctg atccctttgc cgggtgctgt cgcccggcac
     2701 ttaaatctct ttggaaggcg tctcgcaatc tgctgaattt agcggtgaag agtgcacagc
     2761 cgttgcctat actccaggca ggaaactgga ggaaatctca tgagcgaacc cctgttaatt
     2821 gcccgcacgc cggacacaga actgttttta ctgccgggaa tggctaaccg tcacgggctg
     2881 attactggcg caacggggac gggtaaaacc gttacgctgc aaaaactggc agagtcattg
     2941 tcggaaatcg gcgtgccggt gtttatggct gatgtgaaag gcgatctgac cggtatcgcg
     3001 caggcaggaa cggcgtcgga aaaactgctc acaaggctta aaaatatcgg cgtcaatgac
     3061 tggcaaccgc atgccaatcc ggtggtggtg tgggatatct ttggcgagaa aggccacacg
     3121 gtgcgggcga cggtttcgga tctggggccg ctgttgctgg cgcgactgtt gaatctcaac
     3181 gatgtgcaat ctggcgtgct gaatattatc ttccgcattg cggacgatca gggattgttg
     3241 ctgctcgact ttaaagattt gcgggcgatt acccagtaca tcggcgataa cgccaaatcc
     3301 ttccagaatc agtacggaaa tatcagtagc gcatcggttg gtgccatcca gcgcggatta
     3361 ctgtcgctgg aacagcaagg cgcagcacac ttctttggtg agccgatgct ggatatcaaa
     3421 gactggatgc gcaccgatgc caacggtaaa ggcgttatca atatcctcag cgccgagaaa
     3481 ctttatcaga tgccgaaact gtacgccgcc agcctgctgt ggatgctttc agagttgtat
     3541 gaacaattgc cggaagcggg cgatctggag aaaccaaaac tggtgttttt cttcgacgag
     3601 gcacatctgc tgtttaacga tgcaccgcag gtactgctgg ataagattga gcaggtgata
     3661 aggcttattc gctcaaaagg cgtgggcgtc tggttcgttt cgcaaaaccc gtctgatatt
     3721 ccggataatg tgttcggcca gctcggtaat cgtgttcagc acgctttgcg ggcttttacg
     3781 ccaaaagatc agaaagcagt gaagacagcg gcgcaaacca tgcgggccaa tccggcattt
     3841 gataccgaaa aggcaatcca ggaactgggg accggcgagg cgttaatctc gtttctcgat
     3901 gcaaaaggaa gtccttctgt ggtggaacgg gcgatggtga tcgcaccttg ttcgcgaatg
     3961 gggccggtga cggaagatga gcgtaatggc ctgattaatc actccccggt gtatggcaaa
     4021 tatgaagatg aggtggaccg cgagtccgcc tatgagatgc tgcaaaaagg ctttcaggcc
     4081 agtaccgagc agcaaaataa tccccccgtg aaagggaaag aggtggcggt ggatgacggt
     4141 attcttggtg gattgaagga tattttgttt ggcactaccg gaccacgcgg cgggaagaaa
     4201 gatggtgtgg tgcaaacaat ggcgaaaagc gccgctcgcc aggtgacgaa tcagattgta
     4261 cgcgggatgt tggggagttt gctggggggg agaaaaagat aagtcagacg tgatctgt
//
LOCUS       DADLTY010000263         4305 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.267, whole genome shotgun sequence.
ACCESSION   DADLTY010000263 DADLTY010000000
VERSION     DADLTY010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.267"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..208
                     /locus_tag="JN334_004055"
     CDS             <1..208
                     /locus_tag="JN334_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAY7439996.1"
                     /translation="QALAASGVGSARQSASSAAGAVGDKASQNLNSTGSATASSASTA
                     SRATGDARSRSRAAVQRMQERYQR"
     gene            418..1104
                     /locus_tag="JN334_004056"
     CDS             418..1104
                     /locus_tag="JN334_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HAY7439997.1"
                     /translation="MSELTAKALSDSRSFENAILERDMRMKKAGWIVAALAGLLLVFA
                     LAALILLLPLKTTTVELYTLDRQTGRIERVTTVGEDKLDTSEVLNLSQTASYVKRREG
                     YNYFALQQDYRETQMFNSDEVNKEYLDWFNSPDAPDAVFQQAAYVVTVEVLSNVPSEG
                     TGSSMVEMLRIKRTIRKVKDNTETYDYWTVRMTYRYFPQKKMTASEREVNPFGFIVTS
                     YQRFKEKSDE"
     gene            1097..1990
                     /gene="virB9"
                     /locus_tag="JN334_004057"
     CDS             1097..1990
                     /gene="virB9"
                     /locus_tag="JN334_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="HAY7439998.1"
                     /translation="MNKVLMCALLLTAASAWGAATPRGSAYDSRMQNIPYNSQNTTVI
                     NTRAGYITTLVFDADEEVITATAGFPQGWTITPDRNRVGISPAPVKQPVTDADGKTVQ
                     KIFTPTQQDWQTNLFVTTTKRDYSLMLNVIDNDREAGNLAFVVRFSYPDDTRRQNDAA
                     RLARQQELQAAQEKERIQFALKKSTAPRNWNYTRRVAAGSESITPDFAYDDGRFTYLG
                     FSPQKLIPSPTVIVGGSEQVITPTFSTEGNYRVMVLRSLSPRFVLRYGSQVVGIENGS
                     YGKVTVANGSTVSPDVKLESK"
     gene            1987..3183
                     /locus_tag="JN334_004058"
     CDS             1987..3183
                     /locus_tag="JN334_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HAY7439999.1"
                     /translation="MMNEQEDIKTAAELEAEARERARAEIDGDKPAAGAVPPGQPQVV
                     QHAKRRSRRSLVLAAISLVLLVIIATGGDYLMAKLKGDGSGKSASHEPESHATSPARE
                     RNNLGRDLNPLGQLADTTGQEKTTPTDTAAPPQASPPPLTFDKVSALADSAPTTASSA
                     PKSTSSSNVSPAGSASAPGSATPAGQSQDNGLARITGVKRLGLDPDLYIPADRHIPCA
                     MMQRFVSDVGGRFNCLISEDIYSASNAVRLIPAGTEARGLYRTGTLKNGQGRLFLAIT
                     ELRTPEPGRLVIPMVDSQAVGALGENGVAGWIDNHWLERIGNTLLLGTVQDFAAAASG
                     SSPGKDRNTDYTENTRAATAEMAKTLLENSINIPPTMYLNQGDVIGLVTGADIDFSDV
                     YRLRMR"
     gene            3187..4236
                     /gene="virB11"
                     /locus_tag="JN334_004059"
     CDS             3187..4236
                     /gene="virB11"
                     /locus_tag="JN334_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HAY7440000.1"
                     /translation="MDDKLTDTPSEGNVALSHLRDLLFGDYLSREGLTEIAINRPGEL
                     HTKINGAWEMHASPVTLRQCSSFARALAVWNNDTIDETSPILSATLPTGERCQIVVPP
                     ACERNTISITLRKPDYFQRTHQSYIDAGFYDRVQGTEKVESKDGELSRIYLSGNIPLF
                     MEKCVEYGKTLFVVGETGSGKTTYMKMLLHYIPETLRLITIEDNPELKFYSQKNYVHL
                     FYPADAGEGAIVTSDRLVRAIYRMNPDRPLLAEVRGREAWDALKLIESGHEGFMSSLH
                     AGSPRECISGLIDRCYEHPACRGMPVDVLLRKVLHCVDVIVSVDVHGNVRRMSDVYFK
                     PLHMQAMKESFNDAA"
BASE COUNT          924 a         1362 c         1289 g          730 t
ORIGIN      
        1 tcaggcactt gccgcgtcgg gagtaggttc agcccgtcaa tccgcctcct ccgcagccgg
       61 tgcggtgggg gataaagcct cacagaacct taacagcacc ggcagcgcaa cggcctcgtc
      121 tgcctccacg gcctcccgcg ccaccggcga tgcgcgcagc cgcagccgcg ccgccgttca
      181 gcggatgcag gaacgctacc agagataacc cagtctgact gaactgcggg acgccgcaac
      241 ggcgtcctgc ggcttcgtca cgtctttacc acaggaaaaa caccatgaaa tacggcatca
      301 cgctgctgat gctgtgcctg cttgcaggct gcgcacagcg aaacacccaa cttccgcccg
      361 tgtcgggcaa cccggagccg gtcaattccc cggccattat tcaggagtta accccccatg
      421 tctgaactta ccgccaaagc cctgagcgac tcgcgctcgt ttgagaacgc cattcttgag
      481 cgggatatgc gcatgaaaaa ggcgggctgg atcgtggcgg cgctcgccgg tctgctgctc
      541 gtcttcgccc ttgccgccct tatcctgctg ctaccgctca aaaccaccac cgtggagctg
      601 tacacgctcg accgtcagac cgggcgcatc gagcgcgtca ccaccgtcgg cgaggacaag
      661 ctggacacct ccgaggtgct caatctctcg cagactgcgt cctacgtgaa gcgccgcgag
      721 gggtacaact atttcgccct tcagcaggac tacagagaga cgcagatgtt caacagcgac
      781 gaggtcaaca aggaatacct cgactggttt aacagcccgg acgcgccgga cgctgtgttt
      841 cagcaggcgg cctatgtagt cacggtggaa gtcctctcca acgtaccgtc agagggcacc
      901 ggctccagca tggtggagat gctgcgcatc aagcgcacca ttcgaaaggt caaggacaac
      961 accgaaacgt atgattactg gacggtgcgc atgacctacc gctatttccc gcagaagaaa
     1021 atgacggcct ccgagcgcga ggtgaacccg ttcggcttca tcgtcaccag ttaccagcgg
     1081 tttaaggaga aaagcgatga ataaggtact gatgtgtgcc ctcctgctga ccgcagcgtc
     1141 agcatggggg gcggcgacac cgcgcggcag cgcctatgac agccgcatgc agaacatccc
     1201 ctacaacagc cagaacacga cagtgattaa cacgcgggcg ggttacatca cgacgctggt
     1261 ttttgacgcc gacgaggagg tgatcaccgc caccgctggt tttccgcagg gctggacgat
     1321 cacacccgac cgtaaccgcg tggggattag ccccgcgccg gtgaagcagc cggtaacgga
     1381 tgcggacggc aaaaccgtcc agaagatttt cacccccacg cagcaggact ggcagaccaa
     1441 cctgtttgtg accaccacaa agcgcgacta cagcctgatg ctgaacgtga tcgataacga
     1501 tcgcgaggcg ggaaatctgg cctttgtggt gcgcttcagc taccccgacg atacccgcag
     1561 gcagaacgac gcagcccgcc tcgcccgtca gcaggagctt caggccgcgc aggagaaaga
     1621 acgcattcag tttgccctga agaaatccac tgcgccgcgc aactggaact acacccgcag
     1681 ggtggcggca ggttcggagt cgatcacccc ggattttgcg tatgacgacg gccggtttac
     1741 ctatctcggc ttttcaccgc agaagctgat cccgtcgccg accgtcatcg tcggcggcag
     1801 cgagcaggtg atcaccccga ccttcagcac cgagggtaac taccgggtga tggtactgcg
     1861 ctctctttct ccacgctttg tgctgcgcta cggcagccag gtcgtgggga ttgaaaacgg
     1921 cagttacggg aaagtcaccg tggcgaacgg cagcaccgtc tcaccggatg tgaaactgga
     1981 gagcaaatga tgaacgaaca ggaagacatt aaaaccgccg ccgaactgga ggcggaagcc
     2041 cgcgagcggg cgcgggcaga gattgacggc gataaacccg ccgcaggcgc ggtgccaccc
     2101 ggacagccac aggtggtgca gcacgcgaag cgccgcagcc gccgctcgct cgtgctggcc
     2161 gccatcagcc tggtgttact ggtcattatc gccaccggcg gggattacct gatggcgaaa
     2221 ctgaagggtg acggcagcgg aaaaagcgcg agccatgaac cggaatcgca cgccacctcc
     2281 cccgcccgcg agcgcaataa cctcggacgg gatctgaacc cgctgggaca gctggcagac
     2341 acaaccggcc aggagaagac tactccgact gataccgccg cgccgccgca ggcatcaccg
     2401 ccccccctga cgtttgacaa ggtcagcgcc ctggcagaca gcgccccaac cactgcaagc
     2461 agcgccccga aaagcacgtc cagcagtaac gtatcacctg cgggcagcgc gtccgcgcca
     2521 ggctctgcaa cacccgccgg gcagtcgcag gataacgggc tggcgcgcat taccggcgtg
     2581 aagcggctcg gcctcgatcc ggatttgtac attccggccg accggcatat cccctgcgcg
     2641 atgatgcagc gctttgtatc ggacgtgggc gggcgcttta actgccttat cagcgaggac
     2701 atttacagcg ccagcaacgc cgtgcgcctt atcccggcag gcactgaggc gcgcggcctg
     2761 taccgcaccg gcacgctgaa aaacgggcag gggcgtctgt tcctcgccat caccgagctg
     2821 cgcacgccgg aacccggccg cctggttatt ccgatggttg acagccaggc cgtgggggcg
     2881 cttggcgaga acggcgtcgc gggctggata gacaatcact ggctggagcg gataggtaat
     2941 accctcctgc tcggcacggt acaggacttt gccgctgcgg cctccggctc gtcgccgggc
     3001 aaggatcgca acacggacta cacggagaac acccgcgcgg ccacggcgga aatggcaaaa
     3061 acgctcctgg aaaacagcat caacatcccg ccgacgatgt acctcaacca gggtgacgtg
     3121 attggcctgg tcacgggggc ggacattgat ttttcagacg tctacagact gcgcatgagg
     3181 taagcgatgg acgataaact gactgatacc ccgtcagagg gcaacgtcgc gctgtcacac
     3241 ctgcgcgatc tgttgttcgg ggactacctg agccgggagg ggctgacgga aattgccatc
     3301 aaccgccccg gagagctgca cacaaaaatc aacggggcgt gggaaatgca cgcctcgccg
     3361 gtcacgctgc gccagtgctc gtcctttgcc agggcgctgg ccgtgtggaa caacgacacc
     3421 attgatgaaa cgtcgcctat cctctcggcc acgctcccta caggagaacg ctgccagata
     3481 gtggtgcccc cggcctgtga gcgtaacacc atctcaatca ccctgcgtaa gccggactac
     3541 ttccagcgca cccatcagtc gtatatcgat gccgggtttt atgaccgggt gcagggtaca
     3601 gaaaaggtgg aatcaaaaga cggggagctg agccgcatat acctgtccgg gaatatcccg
     3661 ctttttatgg aaaagtgcgt ggagtacggc aaaacgctct ttgtggtggg ggaaacaggc
     3721 tctgggaaaa ccacctacat gaagatgctg ctgcactaca ttcccgagac gctgcgcctt
     3781 atcaccatcg aggacaaccc ggagctgaag ttttacagcc agaaaaacta cgttcatctg
     3841 ttctacccgg cggacgccgg agagggcgca atagtcacct ctgatcgcct ggtgcgcgcg
     3901 atttaccgta tgaaccccga ccgccccctg ctcgccgagg tgcgtggccg cgaggcgtgg
     3961 gacgcactca agctgattga gtccgggcac gaggggttta tgtcctccct gcacgcgggc
     4021 agcccgcgcg agtgtatctc cgggctgatt gaccgctgct acgagcatcc ggcgtgccgg
     4081 ggcatgccgg ttgacgtgct gctgcgcaag gtactgcact gcgtcgacgt cattgtgagc
     4141 gtggacgttc acggcaacgt gcgccgcatg agcgacgtct attttaaacc cctgcacatg
     4201 caggctatga aggagagttt caatgatgct gcctaaactg accggcctga cggcgatcgt
     4261 cgtcctcctg gcgggctgtt cttcgccacc ggagcctgcg gccgt
//
LOCUS       DADLTY010000264         4198 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.268, whole genome shotgun sequence.
ACCESSION   DADLTY010000264 DADLTY010000000
VERSION     DADLTY010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.268"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            84..752
                     /locus_tag="JN334_004060"
     CDS             84..752
                     /locus_tag="JN334_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440001.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="JN334_004061"
     CDS             complement(995..1690)
                     /locus_tag="JN334_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HAY7440002.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="JN334_004062"
     CDS             complement(1683..3110)
                     /locus_tag="JN334_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HAY7440003.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="JN334_004063"
     CDS             complement(3121..3840)
                     /locus_tag="JN334_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HAY7440004.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1119 a         1085 c          869 g         1125 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DADLTY010000265         4183 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.269, whole genome shotgun sequence.
ACCESSION   DADLTY010000265 DADLTY010000000
VERSION     DADLTY010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.269"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(348..995)
                     /gene="ychE"
                     /locus_tag="JN334_004064"
     CDS             complement(348..995)
                     /gene="ychE"
                     /locus_tag="JN334_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HAY7440005.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1472..4147
                     /gene="adhE"
                     /locus_tag="JN334_004065"
                     /gene_synonym="adhC"
     CDS             1472..4147
                     /gene="adhE"
                     /locus_tag="JN334_004065"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAY7440006.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1168 a         1021 c          960 g         1034 t
ORIGIN      
        1 aattcaatca aatagagatt ggctaagcat gtctatcttt tctgattaca gttccagtag
       61 tgaaatgcac aataatttaa caattgatta ttatttagca ctttcctcta ccaaaggcag
      121 tggaattact aatatcattt caattattct tcagcaagcc caggattatg atgtggcgaa
      181 aataacatga tggtgaggag aagcagttaa ggaaatcctt ataactaact gtaatttgag
      241 aagaatattt ttacctattc cttttgcttt tgataactgc gcgggaacaa agtaaaaata
      301 atgacgaagt aaacggagca tggcagctcc gtttcatttg aaaggaatta attaagcagg
      361 ccggggaaaa tccccttaat accagtaacg ataaattcaa tccccaatgc catcagcaat
      421 agccccataa tacgcgtaat cacgttgatg ccggtctggc gtaaaacccg taccagccac
      481 ggtgccatgc ggaacaatcc ccagcaacat aaagcgaaca atgcaatagc cacaaagaaa
      541 ccaaacagat agctaatgct gtgataacgc gtaccccaga cgatggtaga actgatcgcc
      601 cctggccccg ccatcaacgg caacgccaat ggcaccacac caatactttc acgtaccgcg
      661 gtttctgatt tttcttgctt gttctgttta tcctcgccaa gcttgccgct gatcatcgac
      721 atcgctatcg tcaccaccag gatgcccccg gcgatacgga acgaatcaat tgatatacca
      781 aaaagttgta gaatcgtgtc gccgagaaaa agcgagatcc acaagataat ggccacagac
      841 aggttggctg taaggttagt tttgtttcgc gctgccgctg tctgataact ggtcatgctg
      901 ataaagacgg gaataatccc taccgggttg accagcgcaa ataacccgat gaaaaatttg
      961 aagtaaacgg gaaaatcaaa aagggtctga atcacggttc gctccgaagc aaaagccgga
     1021 taatgttagc cataaataag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
     1081 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
     1141 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
     1201 aagcgatgct gaaaggtgtc agctttgcaa aaatttgatt tggatcacgt aatcagtacc
     1261 cagaagtgag taatcttgct tacgccacct ggaagtgacg cattagagat aataactcta
     1321 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
     1381 atcggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
     1441 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
     1501 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
     1561 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
     1621 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
     1681 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
     1741 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
     1801 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gcctgaagac
     1861 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgcaacca acaaagcggc
     1921 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
     1981 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
     2041 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
     2101 cggtgtaggc gcgggtaaca ctccagttgt tattgatgaa actgctgata tcaaacgtgc
     2161 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
     2221 gtctgttgtt gttgttgact ctgtttatga cgcagtacgt gaacgtttcg caacccacgg
     2281 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaatgg
     2341 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
     2401 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
     2461 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
     2521 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
     2581 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
     2641 aacagctcgt atcctgatta acaccccggc ttctcagggt ggtatcggtg acctgtataa
     2701 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tggggtggta actccatctc
     2761 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
     2821 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
     2881 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
     2941 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
     3001 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
     3061 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
     3121 cgccgcgaag atcatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
     3181 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
     3241 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
     3301 tgtaactgac gacgctactg gtcagaaata cccgctggcg gactatgcgc tgaccccgga
     3361 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
     3421 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
     3481 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
     3541 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
     3601 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
     3661 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
     3721 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
     3781 ggctcgccgt cgttatgctg aaattgccga ccacctgggt ctgagcgcac cgggcgaccg
     3841 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
     3901 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
     3961 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct
     4021 gatctccgag ctgaaacaga ttctgctgga tacctactac ggtcgtgatt atgtagaaga
     4081 tgaaactgca gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc
     4141 cgcttaatca gtagcgctgt ctggcaatat aaacggcccc ttc
//
LOCUS       DADLTY010000266         4182 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.270, whole genome shotgun sequence.
ACCESSION   DADLTY010000266 DADLTY010000000
VERSION     DADLTY010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.270"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..186)
                     /locus_tag="JN334_004066"
     CDS             complement(<1..186)
                     /locus_tag="JN334_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HAY7440007.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="JN334_004067"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="JN334_004067"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAY7440008.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="JN334_004068"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="JN334_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAY7440009.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="JN334_004069"
     CDS             3863..4144
                     /locus_tag="JN334_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HAY7440010.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1044 c         1062 g         1060 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagc
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DADLTY010000267         4178 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.271, whole genome shotgun sequence.
ACCESSION   DADLTY010000267 DADLTY010000000
VERSION     DADLTY010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.271"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(452..824)
                     /locus_tag="JN334_004070"
                     /pseudo
     CDS             complement(452..824)
                     /locus_tag="JN334_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            827..1024
                     /locus_tag="JN334_004071"
                     /pseudo
     CDS             827..1024
                     /locus_tag="JN334_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1068..1187
                     /locus_tag="JN334_004072"
                     /pseudo
     CDS             1068..1187
                     /locus_tag="JN334_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            1437..2303
                     /gene="ospB"
                     /locus_tag="JN334_004073"
     CDS             1437..2303
                     /gene="ospB"
                     /locus_tag="JN334_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HAY7440011.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            2632..3372
                     /locus_tag="JN334_004074"
     CDS             2632..3372
                     /locus_tag="JN334_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY7440012.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(3732..>4178)
                     /gene="ospC4"
                     /locus_tag="JN334_004075"
     CDS             complement(3732..>4178)
                     /gene="ospC4"
                     /locus_tag="JN334_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HAY7440013.1"
                     /translation="TDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSL
                     GNKKADIALYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYKVLEYFIN
                     NGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         1271 a          813 c          808 g         1286 t
ORIGIN      
        1 ggggaaaaac gtgtattttt agtcattctg tttacctctt tctcagggcg tttagtctcc
       61 aggatttccg gggcggttca aaaaactctt cgttgattac tgtggactta ctgttggcat
      121 taccgaccct gagaccggag aaataagaac cgcttagatc atcgtggctg ttctcggggt
      181 atcaagttac acatgggcag aggctaccca aggtctacta ttatcgcctg aaagcgctga
      241 tggaacagtg ctatcaggga catgctgatg aaaataccta aataatcagc cagcactcta
      301 tctttccaaa tccacagcat agcaaagaga gcaaaagagc ctgtaaattc agaaattaag
      361 gaaacgcctc ttggtgtaca taatctatct tatgttaaat gaaatctaat ttcttgatta
      421 tatgctggat cgttcgtggg tggctttgcc cctatatttc cagacacctg ttatcattgg
      481 cccgctgccg cagatattcc cgtggcgagc gataatccag cgcactatgc ggatgccatt
      541 cgttataatg ctcgaatgcc tctgcaaggt tctttgctgc cgttaacccg tcaggtttgg
      601 gcatgatact gatgtagtca cactttattg ttttcacgaa gctctttgct attctgttac
      661 tctccggact ccgcaccgcc gtgttcttcg gttcaacaaa gtcccaacat ccgggtgaac
      721 tggcgtgttt cattagctcg gtagcatgaa ccattatccg tcagtcactt cactggagac
      781 gccggaagct cgttgccgaa gcggtgttcc accgctccca gcatgtcgcc aacgggagag
      841 catctacggt ctgattgaac tgtttattca aattgtccac cgtctgtcag tacgctctga
      901 acgtcggctg gttaaaacac tgctcgctga ttttcagagg gttcacggta aaacagcact
      961 tttattccgg attgcggaag cggctctgaa taatccggat gggctggtta aagaggttgt
     1021 gtaccatctg catattgttg aaccagatcg cagcggtaag agaagttcat catacttggc
     1081 gcagttacgc gatgtttctg ctagaggtga tgccgtaaag aacggcagaa cattaccgga
     1141 acaagatagc ggtctacctg cactggtatc agacccgggg cttccccgga tgatctccac
     1201 ggtactttag gtactgtgtc tatattatgt aggtaggatc aattaacttt gtagttgaaa
     1261 ttctttcatt gaaataatga ataaaaaaga caaaccgcat taaacaatgc cagaatataa
     1321 ttgcattttt gttccgtttt tttaaatcat agtatacaaa gaagcttgca tactatatga
     1381 aaatataaca ccctttacta tgatgtttat aaaacaatat atggagtcat gtaggtatga
     1441 atttagatgg tgttagacca tactgtagaa tagtcaataa aaagaatgaa agcatatcag
     1501 atattgcatt tgcacatata ataaaaaggg taaaaaattc atcatgtact cacccaaaag
     1561 cagcattggt ttttttagga gagaaaggtt tttgtgatag caatgatgtt ctatctatta
     1621 tgggacaaca aataccaaga gtatttaaga acaagatgtt atatgattat gtttttaaaa
     1681 atgaaaaaag taaaaatgat tttctaaaaa tggctgaatc atggctacca cagagtgaac
     1741 caatagtaat aaataatgat gatgacgcat tgaatgctgc tgcttatttt tctgtaaaaa
     1801 aagcgaaaat aaaaacagta aacgatactg attttaaaga gtataataag gtttatattc
     1861 ttgggcacgg tagtcctggt tctcatcaat taggccttgg ttcggaactt attgatgtac
     1921 aaacaatcat ttcaagaatg aaagactgtg gtattctaaa tgtgaaagat atccgtttta
     1981 cttcatgcgg ctccgctgat aaagtggctc ctaaaaattt taacaatgcc cctgctgaaa
     2041 gtctttcttg tatccttaac tctctgcctt tttttaagga aaaagaatct ttgctagagc
     2101 agataaaaaa acaccttgaa aacgatgagt cattgagtga tggtctaaaa atatccggct
     2161 atcatggata tggagttcac tatggtcaag agctttttcc ctactcacat tatcgttcaa
     2221 cttcaattcc tgctgatccg gagcatacag taaaaagaag ctctcagaaa aagactttta
     2281 ttattaataa agaactggat tagtataaaa tttttaacct atagttaatt gtttgcattt
     2341 aagatgaagt tattttatct ttagtagaaa agtgcttccc tatgagggaa aatcacaaat
     2401 catcataata cagagacaaa acgatacgaa aaaatagata aaaaacatca ttcttttacc
     2461 acattatttt ccgtgaatat gaaaaataat gttattactt taatataaga ctattttttg
     2521 tttttccatc actctgttca aatttttccg catgacttgt gttttttgta atacagctcg
     2581 ttttttacag ctgaccaaaa tcatcaatta attatgctaa ggaaataaat tatgaaaacc
     2641 aaaaactttc ttcttttttg tattgctaca aatatgattt ttatcccctc agcaaatgct
     2701 ctgaaggcag aaggttttct cactcaacaa acttcaccag acagtttgtc aatacttccg
     2761 ccgcctccgg cagagaattc agtagtattt caggctgaca aagctcatta tgaattcggc
     2821 cgctcgctcc gggatgctaa tcgtgtacgt ctcgctagcg aagatgcata ctacgagaat
     2881 tttggtcttg cattttcaga tgcttatggc atggatattt caagggaaaa taccccaatc
     2941 ttatatcagt tgttaacaca agtactacag gatagccatg attacgccgt gcgtaacgcc
     3001 aaagaatatt ataaaagagt tcgtccattc gttatttata aagacgcaac ctgtacacct
     3061 gataaagatg agaaaatggc tatcactggc tcttatccct ctggtcatgc atcctttggt
     3121 tgggcagtag cactgatact tgcggagatt aatcctcaac gtaaagcgga aatacttcga
     3181 cgtggatatg agtttggaga aagtcgggtc atctgcggtg cgcattggca aagcgatgta
     3241 gaggctgggc gtttaatggg agcatcggtt gttgcagtac ttcataatac acctgaattt
     3301 accaaaagcc ttagcgaagc caaaaaagag tttgaagaat taaatactcc taccaatgaa
     3361 ctgaccccat aaagctggac agcctgtatc aggctatgga gggcccatag acaaatctac
     3421 cctatatgag cataggagga gtctatgggc acaccacgtt ttacccctga atttaaggga
     3481 ttactggaaa ggctgggaca tatccctccg gcagaagcag aaaaagctta ttatgctgcc
     3541 atcggaaacg atgatctggc aacctgagtt cacagataaa acattctcta ggaaactcgg
     3601 ggcggttccg ttcaccacat gcaatgtggt gttgcagggg aacggtctgc accatcccct
     3661 atgtcgatca atataacaga aatgacaact tcagattcag ggcacaacct aaatatattt
     3721 taggtcacct ctcaaatcgt ttgcctgata ccgctccatt ttttaataaa aaaatcaatc
     3781 atttttgaat ctttgctttt cattgcattg tcaagcatag tgtccccact atttgctttt
     3841 tgaaattttt tgtttacatc aaccaatcca ttattgataa aatattctag cactttatag
     3901 ttggcaccat ctttactaag taagtattca acatcataca gattacaata tctattgttt
     3961 ttcattttct ccatctctgc gacatcttgt tttgtaaaat taaatttaga tagtaaataa
     4021 agtgctatat cagctttttt attgcctaaa gaataagcaa gggcctgatg agccattttt
     4081 ttatcagtca ctttattgtt aatctcttca taattagatg ctttaatcgc atcctccaaa
     4141 gatatgtctc tgagttttat ttttttgaag ttatctgt
//
LOCUS       DADLTY010000268         4166 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.272, whole genome shotgun sequence.
ACCESSION   DADLTY010000268 DADLTY010000000
VERSION     DADLTY010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.272"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..332)
                     /locus_tag="JN334_004076"
     CDS             complement(<1..332)
                     /locus_tag="JN334_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HAY7440014.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(332..1714)
                     /locus_tag="JN334_004077"
     CDS             complement(332..1714)
                     /locus_tag="JN334_004077"
                     /inference="COORDINATES: protein motif:HMM:NF012973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HAY7440015.1"
                     /translation="MSMGHAAESYTLLTIGDGLVAQIPALVISTAAGGIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELSAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(1711..2331)
                     /locus_tag="JN334_004078"
     CDS             complement(1711..2331)
                     /locus_tag="JN334_004078"
                     /inference="COORDINATES: protein motif:HMM:NF012973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440016.1"
                     /translation="MILLILSMMVLPLPAFILDLLFTFNIALSIMVLLVAMFTQRTLE
                     FAAFPTILLFTMLLRLALNVASTRIILMEGHTGAAAAGKVVEAFGHFLVGGNFAIGIV
                     VFVILVIINFMVITKGAGRIAEVGARFVLDGMPGKQMAIDADLNAGLIGEDEAKKRRS
                     EVTQEADFYGSMDGASKFVRGDAIAGILIMVINVVGGLLVGVLQLA"
     gene            complement(2356..3549)
                     /gene="flhB"
                     /locus_tag="JN334_004079"
     CDS             complement(2356..3549)
                     /gene="flhB"
                     /locus_tag="JN334_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HAY7440017.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRIFSAQTSAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRICQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVHLLIFRCRQPWILLTRNRPMSNLAAMLRLPA
                     NLKST"
     gene            3785..>4166
                     /locus_tag="JN334_004080"
     CDS             3785..>4166
                     /locus_tag="JN334_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAY7440018.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1037 a         1207 c         1021 g          901 t
ORIGIN      
        1 ccgcctggca cttcccagat aaatcgcaac ggttctgctg ccgctatacc ggaaaaggcc
       61 acggtggtac cgctctgccc ctctaattcg acacaacgag attgcgaaca caagcgaacc
      121 cgcagtcctg caggtgtcgg gccgataagc tgataacgcc acgccaccag cgtcatcaat
      181 cctgaagcag gttgtcgcgc agaaagaggt gcagacgaca tcgactcacc gcgatgattt
      241 agcgtaattc cgacactact tgcctgccac atcccctccc cggcggcttg caccagcagc
      301 ggaaacaata atatcgctaa taaggttctc atcatttacc gccaattgtc gccgtcatgc
      361 ggatatgtcg gttatcagac agttccagat tcgacaggac cactaactgc ggcaagctgc
      421 ggcgcaggaa gcgagacaat aatggtcgca gcgcgtggtt caccaacaat actggcggcg
      481 cacccagcat ctcctgacgg gatagagctt cctgagtttg cgccagtaaa cgatccgcca
      541 gccctggctc cagtcctccc ccgccctgca gcgcctgtag taacaaacgt tccagcggtg
      601 tatcgaggcc aataacatgg acttcatctt tgccaggaaa ccactgctgg gtaatcgccc
      661 gtcccaacgc cacgcgcacg acggcgctta attcatgtgg atcgctttgg atgggcgcat
      721 gttccgccag cgtttcgaga atggtgcgca tatcgcgaat cggtactttt tcatcgagga
      781 gattttgcag cactttatgc agtgtggtga gcgtgacgac gccaggaacg agatcttccg
      841 tcagctttgg catctcctgg gcgacgcgat ccaacagctg ttgcgcctcc tgacgaccaa
      901 acagctctgc ggcatgctgg ctaatgaggt ggttaagatg cgttgctacc accgtgctgg
      961 cctcaaccac tgtgtacccc tgaatctgcg cctgttcttt tagcgcactt tcaatccaga
     1021 tagcattcag gccaaatgcc ggatcgacgg tcgcctcacc aggtaacgtc ccggcagcgg
     1081 ttccagggtt aatcgccagc cagcgccccg gataagcatc accactgcca atctccacgc
     1141 ctttcatcaa aatgcgatag cgggcaggtt gcagatccat attgtcgcga atgtgcacca
     1201 ctggcggcag aaatcccatc tcctgggcaa atttcttgcg gatactgcgt atacggccca
     1261 acaactcacc atcctgctgg aaatcgacca tcgggatcag tcgataaccc acttccattc
     1321 ccagagaatc ttccagttgt acatcgttcc acgtcgcttc gacaacggta ttattctctg
     1381 ccatttttac cggtttgggt tcggcaggcg ctttttgttc gcgtccgcgt atccaccagg
     1441 ccagccccag caatccggca gtgaacagca aaaataccag gttcggcatt ccaggcacca
     1501 ggccgagtaa accgagcacg gcggcgctta acaacataac gcttgggtta ctgaaaagct
     1561 gattcaccat ctgctcgcca acatcctgat cggtgctgac acgcgtaacg atgccccccg
     1621 cggcggtaga aatcaccagc gccggaattt gtgccaccag accgtcgcca atggtcaata
     1681 gcgtataact ttccgccgcg tgtcccatgc tcatgccagt tgcagcacgc cgaccagcaa
     1741 cccgccgaca acgttaatga ccatgatgag gatcccggca atggcatcgc cgcgaacaaa
     1801 cttacttgcc ccgtccattg agccgtaaaa atcggcttcc tgagtcactt cggagcggcg
     1861 ttttttcgcc tcatcttcac caatcaatcc agcgttaagg tcggcgtcaa tcgccatctg
     1921 cttacccggc ataccatcga gaacaaagcg cgcacccact tctgctatac gcccggcacc
     1981 tttggtaatg accataaagt tgatgatcac gagaatgaca aacaccacga taccgatagc
     2041 gaaattgcca ccaacgagga agtgaccgaa cgcttcgacc accttcccag ccgccgccgc
     2101 gccggtatgc ccttccatca aaatgatacg ggttgaagcc acgttaagtg ccagacgcaa
     2161 caacatagta aacaacagaa tggtcggaaa cgcagcaaac tcaagcgtgc gctgggtaaa
     2221 catcgccacc agcaacacca tgatcgacaa ggcaatattg aaggtaaaca acaggtcgag
     2281 tatgaatgcg ggcagtggca gcaccatcat cgacaagatc aacaggatca aaatcggtcc
     2341 ggcaaggatc tgccattatg tcgatttcag gtttgcgggc aggcgcagca tcgcggccag
     2401 attactcatg ggtcggtttc tcgttaataa aatccagggc tgccggcacc ggaagatgag
     2461 taggtgtaca gggcgctgtc caccagccag acgccagcgt ttcagttgcc agacccaggc
     2521 cagcacttcc gccaccgcgg cgtacagttg acccgggatt tgttgaccaa tctccgcatg
     2581 tcgatacagc gctcgcgcca gcggcggcgc ttcaagcgtc gggacgttat tttcagcgcc
     2641 aatttcacga atgcgcagcg cgaccagccc tgcaccttta gcgaccactt tcggtgcgct
     2701 cattttgttt tcgtcatact gcaacgctac cgaatagtgg gtcgggttat tgacaatgac
     2761 atccgctttc ggcacatcgg ccatcatccg acgccgtgcg gcagctcgct gcatctgaca
     2821 gatccgtcct ttaacatggg ggtcgccttc gctttgtttg aactcatcac gaatatcctg
     2881 ccgcgacatt cgcagctttt tcaggtggct gaagatttgg aaaaagacgt caaatcccac
     2941 cattggaatg acaccaagca ccaccagcag tgcgcatagc cctaccaaat ccatcgcatt
     3001 acccatggcg gtaatcggtg actcggccat taagcgcatc atctgcggcc agtgatgcca
     3061 gagaaaaaac cccgtcacgc tgccaaccag gatggttttc aaaattgcct taagcaactc
     3121 cgcgctagtc tgtgccgaga atatccgttt aatgcccggt agcgggttga gtttggaaaa
     3181 cttcggctgc aaggatttgc cgctaaatac cagccccccc agcataaccg gagaaatgag
     3241 cgccaccagc accacgccgc taatcagcgg tagcagcgcc agcatggctt ctctgatcag
     3301 cagaataatt tgcccgagga tcagattcgg gtctttgata atactgtgat caaaatgcag
     3361 cccagcggag agcatgcccg acaatcgacg ggccagcgac acaccgccaa accagataac
     3421 actaacgccc accagcaaaa tcagcagtga ggtcagttca cgggaacgcg ggatttgccc
     3481 ctcttcccgc gctttttcta gtcggtgagg tgtgggggct tctgttttgt cgtcgctctc
     3541 gtcagacacg tcgccaatcc tggaaagagt attaagcgta aatgatgcca gagcgcaaag
     3601 cgttcaatgg tttaagtaag gggcaaaaca ggcgggattt agggcgttta tgcaaaaata
     3661 ggttcagttg cagaacgttt tgcgaaccat tttccagttc tgatatttag ccatttagcc
     3721 tatagaatcg cactccgcct ctctgttagt ttgctaataa ttaaattatt acaaaggtga
     3781 tcaaatggaa tggcaacaat tgcttaactg caaccgtcgg aaagataatc taaaaaaaac
     3841 caacatagca tttttacatc cccttgctag cggaagacaa gagatcgagc gggattacga
     3901 ccgattactt tttgctgcac caactcgtcg gcttgcggat aaaacacaag ttttcccgtt
     3961 agatcaaaat gacagcatac ggacgaggct gactcattct cacgaagtag caaactttgc
     4021 tcgcgggata ggaatgaggc tcgctttcga gatgaaggag cacatctttg gcgaaatacc
     4081 cacggggatt gtcgttgaac gcgacgttcc tgctctgtta gcagcgatcg gactggcaca
     4141 cgatctcggt aatccaccat ttgggc
//
LOCUS       DADLTY010000269         4166 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.273, whole genome shotgun sequence.
ACCESSION   DADLTY010000269 DADLTY010000000
VERSION     DADLTY010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.273"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..2042
                     /gene="fimD"
                     /locus_tag="JN334_004081"
     CDS             <1..2042
                     /gene="fimD"
                     /locus_tag="JN334_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
                     /protein_id="HAY7440019.1"
                     /translation="IGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKW
                     QHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVI
                     HGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFT
                     VPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLA
                     DRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQ
                     LVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTV
                     TQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRD
                     QMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSY
                     SVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANG
                     VTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTL
                     ADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSS
                     GIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
     gene            2052..2582
                     /gene="fimF"
                     /locus_tag="JN334_004082"
     CDS             2052..2582
                     /gene="fimF"
                     /locus_tag="JN334_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HAY7440020.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            2595..3098
                     /gene="fimG"
                     /locus_tag="JN334_004083"
     CDS             2595..3098
                     /gene="fimG"
                     /locus_tag="JN334_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HAY7440021.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            3118..4020
                     /gene="fimH"
                     /locus_tag="JN334_004084"
     CDS             3118..4020
                     /gene="fimH"
                     /locus_tag="JN334_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HAY7440022.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
BASE COUNT         1073 a          967 c         1092 g         1034 t
ORIGIN      
        1 ggattggggg taacagccat tatgcatatt taaacctaca gagtgggtta aatattggtg
       61 cgtggcgttt acgcgacaat accacctgga gttataacag tagcgacagt tcatcaggta
      121 gcaaaaataa atggcagcat atcattacct ggattgagcg agacataatt cctttacgtt
      181 cccggctgac gttgggtgat ggttataccc agggcgatat tttcgatggt attaactttc
      241 gcggcgcaca attggcctca gatgacaata tgttacccga tagccaaaga ggatttgccc
      301 cggtgatcca cggtattgct cgtggtactg cacaggtcac tattaaacaa aatggatatg
      361 gcatttataa tagtacggtg ccaccggggc cttttaccat caacgatatc tatgccgcag
      421 gtaatagtgg tgacttgcag gtaacgatca aagaggctga cggcagcacg cagattttta
      481 ccgtacccta ttcgtcagtc ccgcttttgc aacgtgaagg gcatactcgt tattccatta
      541 cggcaggaga ataccgtagt ggaaatgcgc agcaggaaaa accccgcttt ttccagagta
      601 cattactcca cggccttccg gctggctgga caatatatgg tggaacgcaa ctggcggatc
      661 gttatcgtgc ttttaatttc ggtatcggga aaaacatgga ggcactgggc gctctgtctg
      721 tggatatgac gcaggctaat tccacacttc ccgatgacag tcagcatgac ggacaatcgg
      781 tgcgttttct ctataacaaa tcgctcaatg aatcaggcac gaatattcag ttagtgggtt
      841 accgttattc gaccagcgga tattttaatt tcgctgatac aacatacagt cgaatgaatg
      901 gctacaacat cgaaacacag gatggagtta ttcaggttaa gccgaaattc accgactatt
      961 acaacctcgc ttataacaaa cgcgggaaat tacagctcac cgttactcag caactcgggc
     1021 gcacatcaac actgtatttg agtggtagcc atcaaactta ttggggaacg agtaatgtcg
     1081 atgagcaatt ccaggctgga ttaaatactg cgttcgaaga tatcaactgg acgctcagct
     1141 atagcctgac gaaaaacgcc tggcaaaaag gacgggatca gatgttagcg cttaacgtca
     1201 atattccttt cagccactgg ctgcgttctg acagtaaatc tcagtggcga catgccagtg
     1261 ccagctacag catgtcacac gatctcaacg gtcggatgac caatctggct ggtgtatacg
     1321 gtacgttgct ggaagacaac aacctcagtt atagcgtgca aaccggctat gccgggggag
     1381 gcgatggtaa tagcggaagt acaggctacg ccacgctgaa ttatcgcggt ggttacggca
     1441 atgccaatat cggttacagc catagcgatg atattaagca gctctattac ggagtcagcg
     1501 gtggggtatt ggctcatgcc aatggcgtaa cgctggggca gccgttaaac gatacggtgg
     1561 tgcttgttaa agcgcctggc gcaaaagatg caaaagtcga aaaccagacg ggggtgcgta
     1621 ccgactggcg tggttatgcc gtgctgcctt atgccactga atatcgggaa aatagagtgg
     1681 cgctggatac caataccttg gctgataacg tcgatttaga taacgcggtc gctaacgttg
     1741 ttcccactcg tggggcgatc gtgcgagcag agtttaaagc gcgcgttggg ataaaactgc
     1801 tcatgacgct aactcacaat aataagccgc tgccgtttgg ggcgatggtg acatcagaga
     1861 gtagccagag tagcggcatt gttgcggata atggtcaggt ttacctcagc ggaatgcctt
     1921 tagcgggaaa agttcaggtg aaatggggag aagaggaaaa tgctcactgt gtcgccaatt
     1981 atcaactgcc accagagagt cagcagcagt tattaaccca gctatcagct gaatgtcgtt
     2041 aagggggcgt gatgagaaac aaaccttttt atcttctgtg cgcttttttg tggctggcgg
     2101 taagtcgcgt tttggctgcg gatagcacga ttactatccg cggctatgtc agagataacg
     2161 gctgtagtgt ggccgctgaa tcaaccaatt ttactgttga tctgatggaa aacgcggcga
     2221 agcaatttaa caacattggc gcgacgactc ctgtcgttcc atttcgtatt ttgctgtcac
     2281 cctgtggtaa tgccgtttct gccgtaaaag ttgggtttac cggcgttgca gatagccaca
     2341 atgccaacct gcttgcactt gaaaatacgg tgtcagcggc tgcgggactg ggaatacagc
     2401 ttctgaatga gcagcaaaat caaatacccc ttaatgctcc atcgtctgcg atttcgtgga
     2461 cgaccctgac gccgggtaaa ccaaatacgc tgaattttta cgcccggcta atggcgacac
     2521 aggtgcctgt cactgcgggg catatcaatg ctacggctac cttcactctt gaatatcagt
     2581 aactggagat gctcatgaaa tggtgcaaac gtgggtatgt attggcggca atgttggcgc
     2641 tcgcaagtgc gacgatacag gcagccgatg tcaccatcac ggtgaacggt aaggtcgtcg
     2701 ccaaaccgtg tacggtttcc accaccaatg ccacggtaga tctcggcgat ctttattctt
     2761 tcagtcttat gtctgccggg gcggcatcgg cctggcatga tgttgcgctt gagttgacta
     2821 attgtccggt gggaacgtcg agggtcactg ccagcttcag cggggcagcc gacagcaccg
     2881 gatattataa aaaccagggg accgcgcaaa acatccagtt agagctacag gatgacagtg
     2941 gcaacacatt gaatactggc gcaaccaaaa cagttcaggt ggatgattcc tcacaatcag
     3001 cgcacttccc gttacaggtc agagcattga cggtaaatgg cggagccact cagggaacca
     3061 ttcaggcagt gattagcatc acctatacct acagctgaac ccgaagagat aattgtaatg
     3121 aaacgagcta ttaccctgtt tgctgtactg ctgatgggct ggtcggtaaa tgcctggtca
     3181 ttcgcctgta aaaccgccaa tggtaccgct atccctattg gcggtggcag cgctaatgtt
     3241 tatgtaaacc ttgcgcctgt cgtgaatgtg gggcaaaacc tggtcgtaga tctttcgacg
     3301 caaatctttt gccataacga ttatccggaa accattacag actatgtcac actgcaacga
     3361 ggctcggctt acggcggcgt gttatctaat ttttccggga ccgtaaaata tagtggcagt
     3421 agctatccat ttccgaccac cagcgaaacg ccgcgggttg tttataattc gagaacggat
     3481 aagccgtggc cggtggcgct ttatttgacg cctgtgagca gtgcgggcgg ggtggctatt
     3541 aaagctggtt cattaattgc cgtgcttatt ttgcgacaga ccaacaacta taacagcgat
     3601 gattttcagt ttgtgtggaa tatttacgcc aataatgatg tggtggtgcc cactggcggt
     3661 tgtgatgttt ctgctcgtga tgtcaccgtt actctgccgg actaccctgg ttcagtgcca
     3721 attcctctta ccgtttattg tgcgaaaagc caaaacctgg ggtattacct ctccggcaca
     3781 accgcagatg cgggcaactc gattttcacc aataccgcgt cgttttcacc agcgcagggc
     3841 gtcggcgtac agttgacgcg caacggtacg attattccag cgaataacac ggtatcgtta
     3901 ggagcagtag gaacttcggc ggtaagtctg ggattaacgg caaattacgc acgtaccgga
     3961 gggcaggtga ctgcagggaa tgtgcaatcg attattggcg tgacttttgt ttatcaataa
     4021 aaaaattaca gcgcattgct aatgctggta cctgaggcgc tacgctatca gacctacaaa
     4081 acctgcacgt tagccctttg taggccagat aagacgcgtc agcgtcgcat ctggcataaa
     4141 caaagcgcac tttactggtc tgttcc
//
LOCUS       DADLTY010000270         4125 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.274, whole genome shotgun sequence.
ACCESSION   DADLTY010000270 DADLTY010000000
VERSION     DADLTY010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.274"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            1..437
                     /gene="tnpA"
                     /locus_tag="JN334_004085"
                     /pseudo
     CDS             1..437
                     /gene="tnpA"
                     /locus_tag="JN334_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(555..1499)
                     /gene="hypT"
                     /locus_tag="JN334_004086"
                     /gene_synonym="qseD"
     CDS             complement(555..1499)
                     /gene="hypT"
                     /locus_tag="JN334_004086"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAY7440023.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1844..2563
                     /locus_tag="JN334_004087"
     CDS             1844..2563
                     /locus_tag="JN334_004087"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY7440024.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2629..3927
                     /locus_tag="JN334_004088"
     CDS             2629..3927
                     /locus_tag="JN334_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012847785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY7440025.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1042 a          894 c         1017 g         1172 t
ORIGIN      
        1 gcacacccga tggaactgta aatatcacat agttttttgc gccaaaatac cgaagacagg
       61 tgttctacag agagaagcgt agagcaacag gcagtatttt gagaaagctg tgtgagtgga
      121 aaagcgtacg gattctggaa gctgaatgct gtgcagatca tatccatatg cttgtggaga
      181 tcccgcccaa aatgagcgta tccggcttta tgggatatct gaaagggaaa agcagtctga
      241 tgctttacga gcagtttggt gatttgaaat tcaaatacag gaacagggag ttctggtgca
      301 ggggggacta cgtcgataca gtgggtaaga acacggcgaa gatacaggat tacataaagc
      361 accagcttga agaggataaa atgggagagc agttatcgat tccctgtccg ggcagcccgt
      421 ttacggaccg taagtaacga agttggatgc aaatgtcaga tcgtgtgcgc ctgttagggc
      481 gcggctggta agagagcctt ataggcgcat ttgaaaaacc tccggctatg ccggaggata
      541 tttattgcac gtcgtcaatt gcggcgtgct ggctcttcca ttgatatctg ctggattaaa
      601 tcgtctggca tatgttcctg taaaatacgc cagaggttct cggcggttct attcagtcgg
      661 gtgttcattc ggtaaatata agcctgaatg gggactgtca atttagcagc atcaaggcat
      721 atcagacgtt tggtctgtag ttcatcgacg atcgaccaga tcggtaacca ggcaatgccg
      781 tatccgttga gcgccatatt ctttaataac tcgctcatcg aagagataaa tatcgtgcgc
      841 ccggtaatac ctccaacctc tgccagatga cgattgacca acctgcccat ataagatgtg
      901 gcggtatagt tgagcaaagg aacctggggc tgagtgatat cgaatacagg gtgcccctgt
      961 gcgtcggcgg cacataccgg gtacaactca gattcaaaca gtttcaggca gcaaaaaggc
     1021 gattgcataa gatcttcatc gcgaaacgaa ataataaagt cactcttgcc ttcgcgtaac
     1081 gtatttacgg cctggacaac atcaatggct tcaacatgat agacaaattc tccgccatag
     1141 gcggtaagtg aatgcaccag tttgggtaat acgctcaggg atagtgagtg cgcggctgca
     1201 attttaatgt tgggaacgcc gagtaaactc tggccgttta actcccccaa attgcattca
     1261 agctgctgta ataagctgcg agtctgggaa tgaaagagct tgccctcttc ggttaattgt
     1321 aaaggggtgg tcgtgcgatc gaataaaaca acgccgatcg ctgcctccag cgccttaatt
     1381 cgtcggctga atgcgggttg cgaaagatta cgctcttttg ccgcctgaga aaaatggcga
     1441 caggcttcca gtgtcaggaa atcgtataac cattttgtct cgatattctt cattaccatg
     1501 gcacctgtat tttcaatcca aagacataat aaaacatccc ggcaataaaa ataatgttct
     1561 caattagcac aacctggcct ttatcattcg ttttgtgcat aaccccttca tttccggtat
     1621 tgacgttgat atcacatttc gccaaaaaaa cgaaattttt ccgtcagata accagaatac
     1681 atcatcacta tggtgaataa gcggaatgtt ccatagctgc atcatgcgtg tcttgcatcg
     1741 ccttgtacaa ccttgcattt ctcatcgccc atttcgactg ttaaaaaaga gagcagcagt
     1801 tatttaccgc gtattgtcat taacgttttg agaggcaggt attgtgaaag gtttaattgg
     1861 tgtactcggt ggaatggggc cggcagctac cgtcgacctg tttaataaat ttgtaacttt
     1921 taccgctgca caatgtgatc aggaacatat tcctttaatt atttcttcga tcccggatat
     1981 tcctgaccgt accgatgcat taatgcatca tgggcgttca ccattacccg ttatgcgtga
     2041 ttatatgcat aagctggaag atgccggggc agaatgtatt gttattccct gtaataccgc
     2101 gcattactgg ttcaaagaat taaaagatgc ttgtcatatt gatatattaa gtatcgtaga
     2161 aactacgatc aatgaagttc gcgcctgtgg taaaacccga attggcttgt tagccaccaa
     2221 tgccacgcta tatatgggtt tataccaaaa aggaattgag tcgctgggat ttacctgtgt
     2281 cagccccgat gccgacggtc agaaaaaagt gatggaaagt atttattcct tgaaagctgg
     2341 caatatagcg cacgcacaaa agttgatgaa tgagcaggct gaaatattat tttcccgcgg
     2401 tgcagaaatc cttgttcttg gttgtacaga aattccggtt attctggcgc aagccgttaa
     2461 agagcagcct tcccgctata ttgactcaac ggcgtcactc gttcgtgccg gaataaaatg
     2521 gtacgaaaat cgtgtcggta aacattatct tttaacccaa taatcattta aatcgcagcc
     2581 agaaagttat tcgcttttaa ctgaattata tttataacgg agaacattat ggtttggctg
     2641 gaaattatcg tagtacttgg tgcaatattt tttggtattc gccagggggg aatcggtatt
     2701 ggtttatgtg gcgggcttgg gcttgccatt ctgactctgg gatttggtct gcctatgggg
     2761 tcgccgccag tcgacgtgat cctgattatt atgaccgttg ttgtggcggc ctcagcctta
     2821 caggccgcag gcggaatgga ttatctggta cgcgtcgctg gtaattttat gcggcgtaac
     2881 ccgaaatata tcaacataat cgcgccgatt atcacctggt ctatgaccat tatggccggt
     2941 actgggttta ttgttttttc cacgctgccg gtgattgctg aagtggcgaa agagtccggt
     3001 atccgtcctt cccgcacgct tgccggttcg gttgtcgctt cgcaggttgc gatttccggt
     3061 tcgccgatca gcgccgcaat ggcggctatg ctcactatta tggaggtaaa cggtgtcagt
     3121 tttattcaag tgatgtcggt atgtcttccc acctcttttg ctgccgcgat ggtcgccgct
     3181 tttattgctt cccgacaggg atgtgagttg caggatgacg aggtctacct tgagcgcctg
     3241 caaaaaggac tggtgcaaaa atacgagaat aatagcagca tcaagccagg tgcgacgctc
     3301 tccgtcggat tgttcctgct ggcgacggtc gctattgtta ttcttgctgc cttcccccaa
     3361 ctgcgtccgg gttttgatat cggtaaaccg atggaaacac gcgatatcat tattatctgt
     3421 atgctctctg ccgcctgcct gatggtggtg ctgtgtaaga cggcgaccga tgccattatt
     3481 ctcacctcca cattccgtgc ggggatgagt tcgctggcgg taattctcgg cattgtaacc
     3541 ttaggcacca cttttattga tgcgcattta actgagatta aagatattgc tggtgatatt
     3601 ttgcaggcgt atccgatgtt gttggcgctg gttctttttg gcacttgcgc gctgctctac
     3661 tcgcaaggtg cgaccacgcc gttaattatc ccgcttgccg tcgcgcttga cgtgcccacc
     3721 tgggcaattc tggcgtccta tgtggccgtc accggagtat ttgtactgcc aacgtatcca
     3781 acatcactgg ctgccatgga atttgatact accggcacaa ctcgtgtagg caaatatgtg
     3841 ctgaatcatc catttatgct gcctgggttg gggggaatta ttgctggagt ggcattaggc
     3901 tttgtgattg cgccaatgat tatttagcac tatcaaacat cagtatcgat ggtttataca
     3961 caataaagac agatatggcg gaatatagtc tgttgtaagc gccaacaaag aataggggaa
     4021 ttccgccatg tctgaacatc ttcacaatat ggtattaagt gttgtcgttt aatgatttat
     4081 ttcttggtta tgttgtgtat tagctgcatc tatcgctttt tgata
//
LOCUS       DADLTY010000271         4123 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.275, whole genome shotgun sequence.
ACCESSION   DADLTY010000271 DADLTY010000000
VERSION     DADLTY010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.275"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(150..440)
                     /locus_tag="JN334_004089"
     CDS             complement(150..440)
                     /locus_tag="JN334_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440026.1"
                     /translation="MDATGNTTVTCLSHKMKYKEVACGKQRMHWLALQFVDENNNPVS
                     GLNVQLEYHPRAMAAELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1172..2014)
                     /locus_tag="JN334_004090"
     CDS             complement(1172..2014)
                     /locus_tag="JN334_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HAY7440027.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(2099..2296)
                     /locus_tag="JN334_004091"
     CDS             complement(2099..2296)
                     /locus_tag="JN334_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HAY7440028.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(2316..2804)
                     /locus_tag="JN334_004092"
     CDS             complement(2316..2804)
                     /locus_tag="JN334_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440029.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(2801..3178)
                     /locus_tag="JN334_004093"
     CDS             complement(2801..3178)
                     /locus_tag="JN334_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAY7440030.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            complement(3225..3602)
                     /gene="cbtA"
                     /locus_tag="JN334_004094"
     CDS             complement(3225..3602)
                     /gene="cbtA"
                     /locus_tag="JN334_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HAY7440031.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            complement(3682..3903)
                     /locus_tag="JN334_004095"
     CDS             complement(3682..3903)
                     /locus_tag="JN334_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HAY7440032.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(3990..>4123)
                     /locus_tag="JN334_004096"
     CDS             complement(3990..>4123)
                     /locus_tag="JN334_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAY7440033.1"
                     /translation="PSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
BASE COUNT          976 a          975 c         1082 g         1090 t
ORIGIN      
        1 tcattctgat ctaaaaccat atcttaaaaa aatgcatggg tattttaatt attttgatgt
       61 tgatctaacg cactcaagga ccattttatt accgattatc cattctattt aactgttgcg
      121 atataaatgt ttgttttagg tgctttaatt cagtacaagt catcaaaccg taccagaccc
      181 tgactgtcag tgacaccgac aggtgggggc ggcggtgtgg ggtcaaactg agtgtgtctg
      241 cctctggccc acagagccag ctctgccgcc attgcccgtg gatgatactc cagctgcacg
      301 ttgagaccgg aaactgggtt gttgttctca tccacgaact gcagcgccag ccagtgcatc
      361 cgctgcttac cgcaggcgac ttctttgtac ttcattttgt gactcaggca ggtgactgtc
      421 gtgttgcctg ttgcgtccat tttgcggtta aaccctttta attcaatttg ttaaattgct
      481 ttccaccttt tgcggggcgc gacttaacca aaatattcac tgttatcata atatgcaatg
      541 ggctggaggt tgcaggtcat ttagggattt tctgtaagcc ccgttttgca gaacgttcta
      601 tctccgattg agatcctgtc tctcggtatt accggtagtc tggccataat cccctatgga
      661 tcatattgat atagtaggca taacacccaa aagttgacta aagctaaatc aatcagagtg
      721 aaaaatgttc ggtttgatgt tgggggggac tgaggagggg ccaacgcagg tggtatgcgt
      781 tttacttcag accggacaaa gaccggatgt atccgtgcag taatgaatcc gttagccagg
      841 tgctcatgtg gaaatagtgt ctattgggga tttgaagttt gacagaaaag gccacagtga
      901 ccagaagggg gctacttaga agaaatgttc aggctgggtg gaagaggtaa agcagtgaca
      961 tcagcattca aagtgaccgg atatctacca gaaccccaga aagcaaaaaa ccagcccgta
     1021 ggctggttct tctaaatagt ggtgccggac tcggaatcga aaatttcttt tatcatattg
     1081 atattaatgc aatttgtttg gtttgattaa aaatgtaccc tcaattatac ccgcagtccc
     1141 ttttgatatc gaaagggaat cactctcggg atcaggctga gagcacagaa ggaaagtacc
     1201 tggctattat atcgttaatt ctgtcaatca gttccagcct tttgaaagtg atgtgtgcag
     1261 atcccttctt aaagtattta atgatgaaca tctcgtcttc atagcgctct ttaccctgaa
     1321 cggactggat gtgaccatcc agacgaatag tgatgttttc ccggttgtcg gggatcggtt
     1381 tgccgctgaa cagatgtagc attctttcca ggtcgacgag tcggtcagtc tgttgcccgg
     1441 tgataagatg aaacccccac cggtcccacc tcaccaggtt gttgacgata attttactgc
     1501 caaatttaca gggtgagttg gttttgtaat cccagctcag ccctttgaag acgttgatca
     1561 ctccccgttc aaacacttca tctttgttct ggtgcagttg ttcaaatgtg ctcagaatgt
     1621 tcgcttcgct gatttccgga aaattatcat attccagtga cctgtaccat gtgtcccggg
     1681 tctgcgcatc catcagactc agcatcccgg aacgttgcat cagatcacgc cagagttcgc
     1741 gatcaagatt gcgtgtgatg gctttcattg cggtttccgg tttctccatc agccagcaac
     1801 cgcagcgaaa atcctgtttc atggcccagt ccagagccgt tttacctcta atactttctg
     1861 tcattgctga tacttcagcc agttgtcgga taagtgtgtc gatttgcagc aatgtactct
     1921 ttcgtacagc cagaatgtgg cggatactgg ttgagcagat aacgtccgta tgcccgttga
     1981 ccacttccgg acgagaggct gtgatttcag acataatcga tattcctttt tgcagataaa
     2041 cgaaaacgcc agccgtattc aggctggcgt cagtggaatg aagtctggtg agtaagactc
     2101 accggttctg ttgcagaaac tgcagctgac gcagggtacg gatatcttcc ctggcctgtt
     2161 caatacaggg cagcagtgct gccgaatccg ttttatcctc atcgttgtcg aagataatcc
     2221 ctgattcgca gtcaatattg tcctgcagcc acgcaatcag aatatccagc gccgtttctg
     2281 tggttaataa tttcatccag tgtcttttgt cctgattacc agtcgaaagt ggggaacccc
     2341 ggcagacatt ctgcgccggc gtccagatga atgagactga cgccataacg ccgggtgaga
     2401 gtgacgacca gacgacggaa cgttctggac aggcccaggc gtttaagccg cagaaccggg
     2461 tacgaccatg catcggtacg taacagatag ccgctgcctg taaaatgaat ccattctgtt
     2521 tcgccgtcgt cattagtctg gtgtgacagc gagtacagcc aggcgttgtc cgtttccgtg
     2581 atatgtgcgg tgctgcaacg tatgcaggta agggttgtta acggtggtgg tgtactggtt
     2641 gcgtgttggt cggatttatc gaccacgcgg agtgaataac cgttttcaga gaccttgtta
     2701 atcagttcac tgagattaac accatcgacg tcaacgacaa tgcgccccat gttcagtgcc
     2761 tgcacgttaa cgctgtcggc ttccagcgtc agggccagtt tcattgtttc gcttccggat
     2821 gcttcccccg ggtaatgtta tttaccgttc tgtagttatc gcgggtcatc aggccggttg
     2881 cccggcgtgc ccggagaata tcaatgctgt tgattaactg cgacggggct cctgcgctga
     2941 atcccggctg gtcggtacgt accagcgcgt atttttcaac gagaaagttc accgcgtcac
     3001 acagtgaaat tcctgcctca atatgctgct caatcacacg ttcatcagcg aacggtgtgt
     3061 cattcagcgt gaggccatag tgctggtcca gcagtcgggt gagcagtgtc tgccagatgg
     3121 tgatgggaga cgggcagccc gatgcctccc gtacgtgagt gtcgggtaat gtattcatct
     3181 taagttctct gtaacaggtg gttataaagg ggagatatcc ggtgttatac ggtggttgcg
     3241 ggcgctgccg gtgtcggata aacagccaga taaacgtaac cacaggagcc gagggtgtcg
     3301 gcttcgcagg tcagcccttt cgcatacagt gtgacggtat gctgatggcg gggattcagt
     3361 tcaccgccag tgagcatgag ttccagttgt ttcatcagca gcggaaaggc ctggtccagg
     3421 tggtacgcat ctacgtcgct gaacctgcct ctgataccgg cgcggtctgc aaggtaatgc
     3481 aggcggttac cctcctgtac cagtcgggcc ccgaaacagg gcgtcacggt gcagggcagc
     3541 ccccaccagg ggcggtcgtg attgtcgtcg ggaggcgttg tcccggggag tgtgtctgac
     3601 acgataaagt cctcacagaa aatcggttaa gaatggtccg gtagtggcga taattctgct
     3661 catcagaatt cccttccaga ctcagttcag ggtgacgctc atcagccgga catacggacc
     3721 aaaactgtcc ttacggcgtt ctgcaaacac agccagtaca ccgggaatat cctgcacttc
     3781 acggccggta tatgtatccg tgctaccgtg ccagcggtat ttaccggtac agaacggaaa
     3841 aagacgggat gcaggatgct gacggtgaat acgcatggct tcaccacggg tgatgatttt
     3901 catgagaata gtcctcctga atgtgaaagt aagaaacagc tgagaaagag aaactgaagt
     3961 gatatgaagg tgattgtgac ggtgacgggt taaagcagac cgtgttccgc aaaggagaaa
     4021 acctggctgc caccgactat cagatggtcc ggtacccgga tatcaaccag ggccagtgcc
     4081 tgtaccagac gctcagtgat aaggcggtct gccttgctgg ggg
//
LOCUS       DADLTY010000272         4076 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.276, whole genome shotgun sequence.
ACCESSION   DADLTY010000272 DADLTY010000000
VERSION     DADLTY010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.276"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(5..196)
                     /locus_tag="JN334_004097"
     CDS             complement(5..196)
                     /locus_tag="JN334_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY7440034.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            261..584
                     /locus_tag="JN334_004098"
     CDS             261..584
                     /locus_tag="JN334_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAY7440035.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            526..1284
                     /locus_tag="JN334_004099"
                     /pseudo
     CDS             526..1284
                     /locus_tag="JN334_004099"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1262..1792
                     /locus_tag="JN334_004100"
     CDS             1262..1792
                     /locus_tag="JN334_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY7440036.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            1799..2032
                     /gene="mbeD"
                     /locus_tag="JN334_004101"
     CDS             1799..2032
                     /gene="mbeD"
                     /locus_tag="JN334_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HAY7440037.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(2578..2970)
                     /locus_tag="JN334_004102"
     CDS             complement(2578..2970)
                     /locus_tag="JN334_004102"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440038.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          965 a          932 c         1219 g          960 t
ORIGIN      
        1 tgcgtcagag gttttcaccg tcatccccga agcgagcgag gcaactgcgg taaagctcat
       61 cggcgtggtc gtaaagcgat tcacagatgt ctgcctgttc atccgcgtcc agttcgttga
      121 gcttctccag cagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt
      181 tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga
      241 taccgatgaa acgcgagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
      301 tactggaacg ctgtgagggt aaacagctgg cggtatggat gcggcgggtc tgcctgggtg
      361 agccggttgc ccgttccggg aggctgccga cactggcacc gccgttactg cgtcagctgg
      421 ccgccatcgg gaataacctg aatcaggcag cgaggaaggt gaacagcggg cagtggtctt
      481 ccggtgaccg ggttcaggtg gtggccgcac tgatggccat cggggatgag ctgcgccggc
      541 tgcgtctggc cgtcagggaa caggggacgc gggatgatag ttaagtttca tgccagggga
      601 aaaggcggtg gcagtggtcc ggttgattac ctgctgggaa gggagcgtaa ccgggaaggg
      661 gcaacggtgc ttcagggtaa tccggaagaa gtccgggagc tcatcgatgc cacgccattt
      721 gcgaagaaat acacgtccgg tgttctctcg ttcgcggaga aggagctgcc gccgggaggg
      781 cgtgaaaaag tgatggcgag ctttgagcgt gtactgatgc ccggtctcga aaaaaatcag
      841 tacagcattc tgtgggtgga gcaccaggac aaggggcggc ttgagctgaa ttttgtcatt
      901 ccgaacatgg agctacagac cggaaaacgc ctccagccgt actacgaccg cgcagacagg
      961 ccgagaattg atgcctggca gacgctggtg aatcaccatt acgggctgca tgacccgaac
     1021 gccccggaga accgccggac gctgactctc cctgataacc tgccggaaac gaaacaggcg
     1081 cttgctgaga gcattacacg gtggatagac gctctttatc atgtcgggga gataaaaggc
     1141 cgtcaggatg tgattcaggc gctcacagag gcggggcttg aagtggtcag ggtgacgcga
     1201 agcagtatca gcattgcaga cccgaacggc gggaagaata tcagactgaa aggagcattc
     1261 tatgagcaat cttttacaga cgggcgcgga gttcgagaaa aagctgaaag agagagccga
     1321 atctacagag aaaatgctga gcgacgagtt caggaagctc gaagaatctg taagcaaggc
     1381 tgtgacatca aacgagacga aaatcaaaga cgctatagcc ctgttcacag cttcgacaga
     1441 ggaatcactg aaaaaacatc gggaaggggt gaaagaggcg atgatgcagc acaggaagga
     1501 cgtgttaaag ctggcaggga atacgggcat gatgttactg gggatggtca ttttcctgtt
     1561 taccgtgagt ggcgggacgc tctggtatct tggcgggatg atacaggcga acctggaaga
     1621 aatcaggaag cagaacgaga gtctggagaa gctgaacgcg aagacatggg gcgtgcgtta
     1681 tcacgagggg agcaacggac gttttctggt gttgcccgag gggatgaaag ccgaaacgaa
     1741 ctggacgatg gaaaacggga aacagaatgc cgtaaggctg gtacaggagt gacagagcat
     1801 gacggagctg gaaagccatt tgctgaacgc attagagcag ctgcaacagg actatatgca
     1861 gcggctgaac gaatgggaga gcgccttcgt ggaattgcag aaaatgtttt cgcttacgca
     1921 acgggacaac gcgatgctga acgagcgggt catgcagttg agtcagcagg tgctgcactt
     1981 gagcgagcag acagaacgct tgagccagtt atacagcgag aactggagat aagagaggaa
     2041 cggttgatac aggagcgcga acatgcgtta ttcctggaac gggaacgcca gccggaaata
     2101 caggaacgga cactggatgg cccttcgctg ggatggtaat cagagacagg ctaaaatgtt
     2161 gtcagcgcgt ttccggtgca ttttttggga taatggttgg ctcgggggca ccgggaacgc
     2221 gcttcagtca gaccttgctg aaaacacagg aacgagttaa aacgcatacg ggcgcgattc
     2281 tggtgtgtct tttgtcgctg tgttttgcct ctattgccag atcgcgctca ttcccctgaa
     2341 cttctgtttt cccgctcagg tccccgtctt cttcgcttcc gaaagcatga ataaaatccg
     2401 tttcagctgt ctctggctgg caattcctgc gtgattcaca tggctgcata gctatgcatg
     2461 tggttaaaat ttaccgggtg cgatcgcggc agtttttcgg gtggtttgtt gccattttta
     2521 cctgtctgct gccgtgatcg cgccgaacgc gtttcagcgg tgcgtacaat taagatatta
     2581 cgttaaattg tgagggaatg gtccccatat actgacttta tcatagggat agggttggta
     2641 gtgggtcgat tgggtttcgg cattgtttcc gttaatgcca attgcatttt tgtatttgat
     2701 gcaacagaag gcgtgagatt ctcctgggcc tggaaaggct aagtgtttag aattgatggc
     2761 aaaatatctt ccgtccgatt tttgaagtag ttgttgtttg gctgtctccc aaagggtgct
     2821 gttacaaact tcttgaattt gtagtttttg caggactctt attactgcgt tgtcatgttc
     2881 caattcttta ggggagtgta ttattctctc ttttaataag ctgtttacca cagggtttag
     2941 ttgttgtcta aagcacgcag ataatgccat tgctacacaa ttttgttccc gaggaagcat
     3001 gtttggcggg gtatgccgtg tttgatgtga tatgtctatt ctcatagaag gtctctggct
     3061 tatagcatca acaaatacgg ctataaaaca aaattttcat agagtgattc aagatcttct
     3121 tgagatcctt ttttgcggcg cgtaatctgc tgcctgcaaa caaaaaaacc accgctacca
     3181 acggtggttt gtttgccgga atctagagct accaactctt tcttccgaag gtaactggct
     3241 tggtggagca cagataccaa atgctgtcct tctagtgtag ccgcagttag gccaccactt
     3301 caagaactct taatatctca atccaccttg tccagttacc aatggctgct gccagtggcg
     3361 ttaagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3421 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3481 tgagatacca acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3541 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3601 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcggt
     3661 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccatcggc gcggccttct
     3721 tcctgttcct ggtcttttgc tcacatgttc tttccggcgt tatcccctga ttctgtggat
     3781 aacagcattt cgtttttgag tgagtagata ccgctcgccg cagtcgaacg accgagcgta
     3841 gcgagtcagt gagcgaggaa gcggaaaagc gcctggacgt gcattttctc cttacgcatc
     3901 tgtgcggcat ttcacacccg gtatggcgca cttttcatac aatccgcact gatgccgcat
     3961 ggttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
     4021 cccgctaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgctc
//
LOCUS       DADLTY010000273         4019 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.277, whole genome shotgun sequence.
ACCESSION   DADLTY010000273 DADLTY010000000
VERSION     DADLTY010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.277"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..1331
                     /gene="phrB"
                     /locus_tag="JN334_004103"
     CDS             <1..1331
                     /gene="phrB"
                     /locus_tag="JN334_004103"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAY7440039.1"
                     /translation="VLALYIATPRKWETHNMSPRQAELINAQLNGLQIALAEKGIPLL
                     FREVDDFVASVEIVKQVCAENSVTHLFYNYQYEVNERARDVDVERALRNVVCEGFDDS
                     VILPPGAVMTGNHEMYKVFTPFKNAWLKRLREGMPECVAAPKVRSSGSIKPAPSITLN
                     YPRQSFDTAHFPVEEKAAIAQLRQFCENGAGEYEQQRDFPAVEGTSRLSASLATGGLS
                     PRQCLHRLLAEQPQALDGGAGSVWLSELIWREFYRHLMTYYPSLCKHRPFIAWTDRVQ
                     WQSNPAHLQAWQKGKTGYPIIDAAMRQLNSTGWMHNRLRMITASFLVKDLLIDWREGE
                     RYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRIFNPITQGEKFDREGEFIRRWLPE
                     LRDVPGKAVHEPWKWAQKAGVKLDYPQPIVEHKEARVQTLAAYEAARKGK"
     gene            complement(1481..2962)
                     /gene="dtpD"
                     /locus_tag="JN334_004104"
     CDS             complement(1481..2962)
                     /gene="dtpD"
                     /locus_tag="JN334_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HAY7440040.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            3233..3976
                     /gene="ybgI"
                     /locus_tag="JN334_004105"
     CDS             3233..3976
                     /gene="ybgI"
                     /locus_tag="JN334_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HAY7440041.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
BASE COUNT         1034 a          967 c         1117 g          901 t
ORIGIN      
        1 gcgtgctggc gctgtatatc gctacaccac gcaagtggga gacgcataac atgtcgccgc
       61 gtcaggctga acttatcaat gctcaactga atgggctaca aatcgcgctg gcggaaaaag
      121 gtattccttt attgttccgt gaagtggatg actttgtcgc cagtgtcgaa atagttaaac
      181 aggtgtgcgc ggaaaacagc gttactcatc ttttttataa ctatcagtat gaggtgaatg
      241 agcgagcgcg ggatgtggac gttgaaagag cgctgcgtaa cgtggtgtgt gaaggatttg
      301 atgacagcgt gatcctgccg ccgggggcgg tgatgaccgg caatcacgag atgtacaaag
      361 tctttacgcc ttttaagaat gcctggctga aacggctgcg ggaagggatg ccggagtgcg
      421 tcgctgcacc aaaagttcgt agtagcggat cgataaagcc cgcgccatcc attacgctga
      481 attatcctcg tcagtctttc gatactgcgc atttcccggt ggaagaaaaa gcggcgattg
      541 cgcaattacg ccagttttgc gagaacggtg ctggagagta tgagcaacaa cgagattttc
      601 cggcagtgga aggcaccagc cgtttgtccg ccagcttggc aacgggcggg ttatcgcctc
      661 gccagtgttt gcatcgcttg ttggcggaac agccgcaggc gctggacggt ggggccggta
      721 gtgtctggct tagtgagctg atctggcgcg agttctaccg tcatctgatg acgtattacc
      781 cctcgttgtg taaacatcgt ccatttattg cctggacgga tcgtgtgcag tggcaaagca
      841 atcccgcaca tttacaggcc tggcagaaag gcaaaacggg atacccgatt attgatgccg
      901 ctatgcgtca gcttaacagc actggctgga tgcataaccg gctacggatg attacagcca
      961 gttttctggt gaaagatttg ttgatcgact ggcgcgaagg cgagcgatat ttcatgtcgc
     1021 agctgattga tggtgatttg gcagctaata acggtggctg gcagtgggcg gcttctacgg
     1081 gtactgatgc tgcgccgtat tttcgtattt tcaatccgat aactcagggc gagaaatttg
     1141 accgtgaggg cgagtttatt cgtcgatggt taccggagct gcgcgatgta ccagggaaag
     1201 cggtgcatga gccgtggaag tgggcgcaga aagcaggtgt gaagctggat tatccgcaac
     1261 cgatagtcga gcacaaagag gcgagagtac aaacgttggc agcgtatgag gcggcgcgga
     1321 aggggaaata acagacgcgt caggcaatcg agcccagatg ccggatgcgg cgtgaacgcc
     1381 taatccagcc tacaaatttg tgcaaattca ataaattgca ggaaatacgt aggcctgata
     1441 agacgcgtca agcgtcgcat caggcatcgg tgctggccta ttatgactcc agcgccagcg
     1501 cgcggtttct gaatttcagc gcctgataca gccaaatcat cagtaccagt cctacacacg
     1561 ccagtgcgcc ccaggtaatt tgatcaaata cttcaatata tgcattgatg gagtagttga
     1621 tcgccccgga agcatcaaac gaagcctgcg atgtctgatc ggcaatcacg cccgccagat
     1681 agttcgcgat cgcgccagaa agcagcatgt agatgccggt taatacgccg gtcacgcccg
     1741 gaatttcaat gcgcgtaatt tgcgacatgg caaccgggtc gataaacagt tccgcaaagc
     1801 ccatcaccgc gaggcctaac accatcagtg gcagagaaga gtgaccatac attgcggacc
     1861 agcgggcgct taaggtcaga atgcagaatc cggcgctcat caggccaagg ccaagagcaa
     1921 attttcccca gatgcgcacg gtacgattac ctgcgacgct ttctttaacc acccacgcca
     1981 ggaacacacc gcacagcata actgcgaagg cattaatcga ctggaacatc gcggtcggaa
     2041 cggtataacc aaacatatcg cggttaacga agcggtcgat ataaaggcta atcgagctac
     2101 cgccctgttg tgcgaaggcc cagaacaaca tactgaagaa ggtgagcgtc acaatcagcc
     2161 ccagctcctt ccgctgtttc tggttttctg ctttgcgata aatttttgcc agtacaccca
     2221 ggccaataat agtcgcgaca attaaggcgt ataccgacca ctctttccag aacagcacgg
     2281 taatcagtgc aggcgttgcc accagcagaa ccagcagcca tccccagttc ggcaggagaa
     2341 agtttgtcgc acgcagtact tttttgttaa cgccgcgggt atgagtgaaa tgacgattgc
     2401 cacataagaa aatgaccaga cccgcgatca ttccaaccgc cgccaggcca aagcccatcg
     2461 cccagctgta ctcttcctgg gcgtaaccac aggcgatagg tgcgataata gaccccacgt
     2521 tacccgccgc atacatcagc gagaaaccgc catcacgacg cggatcggtt ggctcataca
     2581 gctcgccgag cagacagctt acgttagatt taaacaggcc atagccgcag acgataatcg
     2641 ccagggacag atagaggaat gacggatgga tctcactggc acccagcacc acatgaccga
     2701 tcgccatcaa caacgccccc agcatcaccg ccatgcgatt gccgagaact ttatccgcca
     2761 aaaagccacc gaggattggc gtgacataca ccagcgaaca gtaggcgcta aataactcgt
     2821 aggcgtgagt atcgttgtat tttagttgat tggtgagata gagaatcagc agggcacgca
     2881 tgccgtaaaa gctaaaatat tcccagattt gcagcgcaac gacatagtaa atagcgcgcg
     2941 gttgtgatgc gtgtttattc ataataattc cacaattgac ggcaacgtca cgtaatagtt
     3001 gcgcggcgtg cttgtgtttc cttaaagtta aaactttgat acagatctga ttatttatgc
     3061 aatatgctgt ctgattgcat aaatatacat tagctgaagc gtgatgatat aaacaatttg
     3121 cgtcttttgg cgtcgggttt gtttcttaat atgtcaaaag attggctatc gacgaacagg
     3181 cgatgtaacg ctatgttaac ggtgtagcta tcagaatacg gaaggcgcga agatgaaaaa
     3241 caccgaactg gaacaactga ttaacgaaaa actgaacagc gcggcgatta gtgattacgc
     3301 gccgaatggt ttgcaggtgg aaggcaaaga gacggtgcaa aaaattgtta ccggtgtcac
     3361 cgccagccag gctttgctcg atgaggcagt gcgtctgggc gcggatgcgg tcattgtgca
     3421 tcatggctac ttctggaaag gagagtctcc ggtcattcgc ggaatgaagc gtaaccgttt
     3481 aaaaacgttg ctggcgaatg atatcaacct gtatggctgg catttgccgc ttgacgcaca
     3541 tcctgagctg ggcaataacg cacagctggc ggcgttactg gggatcacgg tcatgggcga
     3601 aattgagccg ttggtgccgt ggggagagct gaccatgcct gtgccgggaa tggaactagc
     3661 ctcctggatt gaagcgcgtc tgggacgtaa gccgttatgg tgtggcgata ccggacctga
     3721 ggtggtccag cgcgtcgcct ggtgcacggg cggcgggcaa agttttatcg atagcgccgc
     3781 gcgttttggc gtggatgctt ttattactgg cgaagtttct gaacagacca ttcattcagc
     3841 ccgcgagcag ggattgcatt tttatgctgc aggtcaccat gccactgaac gcggtggtat
     3901 tcgcgcattg agcgagtggc tgaatgaaaa taccgatctt gatgtgacct ttattgatat
     3961 tcctaatcct gcataacgaa taatcagagg gatcgaaagt gcaacgagcg cgttgttat
//
LOCUS       DADLTY010000274         3873 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.278, whole genome shotgun sequence.
ACCESSION   DADLTY010000274 DADLTY010000000
VERSION     DADLTY010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.278"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..281)
                     /locus_tag="JN334_004106"
     CDS             complement(<1..281)
                     /locus_tag="JN334_004106"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308157.1"
                     /note="catalyzes the interconversion of bicarbonate and
                     carbon dioxide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY7440042.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHI"
     gene            390..1316
                     /locus_tag="JN334_004107"
     CDS             390..1316
                     /locus_tag="JN334_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7440043.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            1313..2083
                     /locus_tag="JN334_004108"
     CDS             1313..2083
                     /locus_tag="JN334_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7440044.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            2188..2628
                     /locus_tag="JN334_004109"
     CDS             2188..2628
                     /locus_tag="JN334_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY7440045.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            2692..>3873
                     /locus_tag="JN334_004110"
     CDS             2692..>3873
                     /locus_tag="JN334_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY7440046.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TD"
BASE COUNT          949 a          879 c         1010 g         1035 t
ORIGIN      
        1 ataatgtgtt caacttcgag tacatccact gcatactgaa ccacggaaag gcagttcagg
       61 tcggtgtgaa tgactaggtt agcaacatta cggtgaacaa agagttcgcc cggctcaaga
      121 ccggttaaac gttctgcagg aacgcgactg tcggaacatc caatccatag aaagcgcggt
      181 ttttgcgctt gtgccagttt ctcaaaaaac ccgggatcct cttccaccag catttttgac
      241 catagtgcat tgttgctgat gagtgtatct atgtctttca tggaggttaa cgacctgtaa
      301 ccaaataatt acgtttggct aatatagggc aactccggga cgatttaaac cacagataaa
      361 gtgtaagaac gtaaggtaag taaaaattta tgaccattgc actggaactt caacagctta
      421 aaaaaaacta tccaggcggc gttcaggcgc ttcgtggaat agatttgcag gtcgaagcgg
      481 gtgattttta tgcgcttctc gggccgaacg gggccgggaa atcgaccact atcggtatta
      541 tcagctctct ggtaaataaa acctccgggc gggtcagcgt atttggttac gatctcgaga
      601 aagatgtcgt gaacgctaaa cgtcagttgg gactggtgcc gcaggaattt aacttcaacc
      661 cgtttgaaac cgtgcagcaa attgtggtga atcaggcagg gtactacggt gtggagcgca
      721 aagaggcgta catccgcagc gaaaaatatc ttaagcagct cgatttatgg ggaaaacgca
      781 acgaacgtgc gcgtatgtta tctggcggga tgaagcgccg tttaatgatt gcccgtgcgt
      841 taatgcatga acctaaacta ctgattctcg acgaaccgac cgcaggcgtg gatattgaac
      901 ttcgccgctc aatgtggggc tttttgaagg atttaaacga caaaggcacc accatcattc
      961 ttaccacaca ctacctggaa gaagcagaaa tgctgtgccg caatatcggc attattcaac
     1021 atggtgagct ggtggaaaat acctcgatga aggcgctgct ggcgaagctg aaatcggaaa
     1081 cctttattct cgatctcgca ccgaaaagtc cgttaccgaa gctcgatggc tatcagtatc
     1141 gactggtcga taccgcgacg ctggaagttg aagtgctacg tgagcagggg atcaacagcg
     1201 tatttacgca gttaagtgag cagggcattc aggtattaag tatgcgtaac aaagctaacc
     1261 gtctggaaga gctgtttgtt tcactggtta atgaaaaaca aggagatcgc gcatgatgca
     1321 tctttactgg gtggcgctaa aaagcatctg ggcgaaagag atccatcgct ttatgcgtat
     1381 ctgggtgcag acgctggtgc cgccagtcat caccatgacc ctttacttta ttatcttcgg
     1441 taacctgata ggttcgcgta ttggcgatat gcatggcttc agctatatgc agttcatcgt
     1501 accggggctg atcatgatgt cggtgatcac caacgcctac gctaacgtcg cgtcatcatt
     1561 ttttggtgcc aagttccagc gtaatattga agagctgctg gtagcgccgg ttccgactca
     1621 cgtcattatt gccggatatg tcggcggtgg cgtggcgcgt ggtctgtttg ttggcattct
     1681 tgtgacggca atttcactgt tttttgtgcc gtttcaggtg cattcgtggg tattcgttgc
     1741 cttaaccctg gtgctcacgg cggtgctatt ctcccttgcg ggtttgctga acggcgtgtt
     1801 tgccaaaacg ttcgatgaca tcagcctggt gccaaccttt gtgttaacgc cactcacgta
     1861 tttgggcggg gtcttttact cactgacttt gttgccgccg ttctggcaag ggctgtcgca
     1921 cctgaaccca atcgtttata tgatcagtgg tttccgctac ggcttcctcg gtataaatga
     1981 tgtgccgctg gtcactacct ttggcgtact ggtggtcttt attgtggcgt tttatttgat
     2041 ctgttggtcg ctgatccaac gtggacgtgg tttgcgtagc taaggctatt tcctctcctc
     2101 tggattcggg ggagaggagt tttgacggct atcacccttt atcaacaatg gtcagggtag
     2161 gctgattttc ggctaaggag gaaggcgatg ttaggttggg taattacctg tcacgatgac
     2221 cgggcgcaag agatactgga tgcgctggag aaaaaacatg gggcacttct tcagtgccgg
     2281 gccgtgaatt tctggcgcgg attaagctct aatatgctca gccgcatgat gtgcgatgct
     2341 ctgcatgaag cggactctgg tgagggtgtc atcttcttaa ccgatatagc cggagcgcca
     2401 ccgtatcgcg tggcttcatt attaagccac aaacactccc gttgcgaagt gatttctggt
     2461 gtcacgttac cgttaattga acagatgatg gcttgccgtg aaaccatgac cagttcagag
     2521 tttcgtgagt gtattgtcga actgggtggg ccggaggtga gtagtctttg gcaccaacaa
     2581 caaaaaaatc cgcctttcgt cctcaaacat aatttgtatg agtattaacc cgcgattctg
     2641 atggcgcttt tgctacaata aaagcgttgt ttcaccctcg gttatttttt catgtataaa
     2701 caagctgtta ttctcctgct gatgctgttt accgcaagtg tcagtgccgc gttacctgcc
     2761 cgttatatgc aaaccatcga aaatgctgcg gtctgggcgc aaattagtga caagatggtg
     2821 accgtgggga atattcgggc cggacaaatc attgccgtgg agcccactgc cgcaagttat
     2881 tacgcattta attttggctt tggtaaaggg tttatcgata aagggcatct cgagccagtt
     2941 cagggacgac aaaaagttga agacggtttg ggtgacctca acaagccgct gagtaatcag
     3001 aacttaatta cctggaaaga tacgccggtc tacaacgcgc cgagtgtggg cagtgcgcca
     3061 tttggggtac tggcggacaa tttgcgctac ccgattttgc ataaactgaa agacaggtta
     3121 aatcaaacct ggtatcagat ccgtattggc gatcgactgg cctatatcag cgcgctggat
     3181 gcccaacccg ataatggcct gccggtgcta acttatcacc atattctgcg cgacgaagaa
     3241 aacacccgtt ttcgccatac ttcgacgacc acctcggtac gcgctttcaa taaccaaatg
     3301 gcctggctgc gcgacagggg atacgcgaca ctgagcatgg ctcagctgga aggttacgtg
     3361 aagaatacga tcaatctccc tgcgcgagcg gtggtgatta cctttgatga tggcctcaag
     3421 tcggtgagcc gctatgcgta tcctgtgttg aaacaatatg gcatgaaggc gacggtgttt
     3481 attgttacct cacgcatcaa acgtcacccg cagaagtgga acccaaaatc gctgcaattt
     3541 atgagcgttt ctgagcttaa cgaaattcgc gatgtttttg atttccagtc acatacccat
     3601 tttttgcatc gggtagatgg ttatcgccga cccatattac tgagccgtag tgagcacaat
     3661 attctgtttg attttgcacg ttcacgccgc gctctggcgc aatttaatcc acatgtctgg
     3721 tatctttcgt atccgtttgg cggctttaat gacaaagccg tgaaggcagc aaacgatgcc
     3781 ggatttcacc tggcggtgac aaccatgaaa ggcaaagtaa aaccggggga taatccgttg
     3841 ttactaaaac gactttatat cttaagaacg gat
//
LOCUS       DADLTY010000275         3816 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.279, whole genome shotgun sequence.
ACCESSION   DADLTY010000275 DADLTY010000000
VERSION     DADLTY010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.279"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..322
                     /locus_tag="JN334_004111"
     CDS             <1..322
                     /locus_tag="JN334_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440047.1"
                     /translation="TLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(386..901)
                     /gene="luxS"
                     /locus_tag="JN334_004112"
     CDS             complement(386..901)
                     /gene="luxS"
                     /locus_tag="JN334_004112"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAY7440048.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(1051..2607)
                     /gene="gshA"
                     /locus_tag="JN334_004113"
     CDS             complement(1051..2607)
                     /gene="gshA"
                     /locus_tag="JN334_004113"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY7440049.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(2680..3108)
                     /locus_tag="JN334_004114"
     CDS             complement(2680..3108)
                     /locus_tag="JN334_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7440050.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(3105..3671)
                     /gene="yqaB"
                     /locus_tag="JN334_004115"
     CDS             complement(3105..3671)
                     /gene="yqaB"
                     /locus_tag="JN334_004115"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HAY7440051.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT          958 a         1023 c          899 g          936 t
ORIGIN      
        1 aacgctggcg gggtctatcg gcacgtcgat aaccacgacc atgtggacca accgcgagtc
       61 gatgcaccat gcgcagttga ctgagtcggt aaacccgttc aacccgaatg cccaggcgat
      121 gtacagtcaa ctggaagggc ttgggatgac gcaacagcag gcatcaggct ggattgccca
      181 gcagatcacc aatcaggggc tgattatttc cgccaatgag atcttctgga tgtcagccgg
      241 gatattcctc gtactgctgg ggctggtgtg gtttgctaaa ccgccatttg gcgcaggtgg
      301 cggcggaggc ggtgcgcact aagtacaact aagccagttc atttgaactg gcttttttca
      361 attaattgtg aagatagttt actgactaga tgtgcagttc ctgcaacttc tctttcggca
      421 gtgccagttc ttcgttgctg ttgatgcgta cctcacgttc cagaatgcta cgcgcaatat
      481 cctgcgcttc ctgcaacgag tgcatctggt aagtgccaca ctggtagacg ttcagttccg
      541 ggatctgatt ctgatcctgc actttcagca cgtcttccat tgccgctttc caggcatcag
      601 caacacgctg ctcatctggc gtaccaatca gactcatata aaaaccggtg cggcagccca
      661 ttggcgagat atcgataatc tctacaccat taccgttaag atggttacgc ataaaaccag
      721 caaacaggtg ctccagggta tggatccctc tttctggcat cacttctttg ttcggcacgc
      781 agaagcgcag atcgaacacg gtgattgcgt cgccatgcgg ggtgttcatt gttttcgcca
      841 cccgaactgc aggcgcttcc atccgggtat gatcgactgt gaagctatct aacaacggca
      901 tttagccacc tccggtaatt tttttaaaaa ttttctgaac tctttcttcc caggcgagtc
      961 tgagtatatg aaagacgcgc atttgttatc atcatccctg aattcagaga tgaaattttg
     1021 gccactcccg agtggccttt ttcttttctg tcaggcgtgt ttttccagcc acaccgcaaa
     1081 cggttcggta tcagcggttt ccatttcctg ctgacggcgt tcagatgcct cgcgctcggc
     1141 tacaaaatcc tcttcgcgca gaatttccag cggctcttca cgcagcagat tacggtaggc
     1201 ttctgcaaac gctttgcctg ttccgccaat accagtatca atcatagacc ttaagatacg
     1261 ggcagagaaa gtcagatcgg gattatcgaa gcaggcaacc agttcatcac acactttctg
     1321 atacgcttcg ccgccgttaa tgctatccag cgtttgcgcg acgcgtttca gatcgcggaa
     1381 cagatcttta cccacctgca gtaacgggaa ctgcgcggtt tcgcagccga tacccagcgt
     1441 caggcccggt ttgcgacctt caaggatcac ccggttccag ttaacgcgcg tacaggcaag
     1501 ttcgctactg ctcatttccg gtgcatcagc cagcgcacac cagaccataa acaggtcgag
     1561 gaatcgcacc tgctgttcat ctacaccaat cggcgagaac gggttgatgt ccagcgaacg
     1621 cacttcaata tattcaatgc cgccacgtaa cagcgcatca gaaggtgact cgccgctgcg
     1681 ggtaacgcgt tttggacgaa tcggcgcata cagttcgttt tcaatctgca gcacgttgct
     1741 attgatttgc agcctcttac cgtctttctc aataccaatc ttcgcgtact cttccgatgg
     1801 cgttttgatt gcctgtttaa ggcccgctac gtactcgtaa agatcgttga aggtaatacc
     1861 aagattgctt tgcgatttat tggtatagcc gagatcgctc aaacgaagag aggtcgcata
     1921 cggcaggtaa tacataccgc actcggtttt ctcaaacggc agcgacgttg gttttccttg
     1981 caggaaagaa gaacaaatcg ccggagatgc accaaacaga taaggaataa cccaaccgaa
     2041 acgatagtaa ttgcggataa cgcggaaata gcccgcagaa attttctctt tggcatcagc
     2101 gcccgagata tcaccgcact tcgcttgcca gaatgccatt ggcaaagaga aattgtagtg
     2161 cacgccggaa atggtttgca tcagcgcgcc gtagcgattt ttcagccctt cacgatacag
     2221 cgttttaaag cgtccggtgt tagaagtgcc gtactgtgcc agttcgatgt cctgaccttc
     2281 tgcgatgtag catggcatac ttaacggcca catccgctca tcgcccatat tgcgcgccgt
     2341 ataacgatgc agatcgcgca taaaggtcag catatgttca atatcaccat ccactggtgt
     2401 aatgaattcc agcaatgctt ccgcaaaatc ggtagtaatc catttgtgcg tcagtgcgga
     2461 acctaatgct tcaggatgac ctgttgttgc cagtgtgcca tcagcattaa cacgcaaagt
     2521 ttcgcgctcc agcccacgct gtatcccctt taacgcctga ggatgttttt ccagccaggc
     2581 cagcgcctgt gatacgtccg ggatcaaatt gacctcccgc ctgtcaaaat cgttttaatt
     2641 agcataactg taatggtgac catatgtgca ggcctacaat tagtgccacc acatcatgcc
     2701 ctgaacggtc gctgctgcaa ctgcaacata gcgtaacgct ttaccaaggc acaaaaaaaa
     2761 gattactggc ccccaggaga tgcgcatcca tcccgctaac agacacagta aatcgccaac
     2821 cacgggcatc cagcttaata atagcgtgac tgcaccatag cgtttcagcc agccggtagc
     2881 tttctcttgc cagcgcgatg ttttacgcaa tggaaagaaa cgcccaagga taacgttagt
     2941 taaccctcca aggctattac ccattgttgc tgttaaaact aaaacccagg gatgactgat
     3001 cccggaaagc aacattgcca ccagcacgac ttcggagttg ccgggtaata gtgtagcgct
     3061 gagaaaacta ctggcaaaca acgagaaaag cgataacgct tcactcacag caagcgaaca
     3121 tccacagcgt ccatgcctgc tgcacgggcc gcctgaatac cgaaatcggc atcttcaaag
     3181 accacacact gcgtcggttg cacgcccata cgctgcgcgc acaacaaaaa tgtgtctggc
     3241 gcgggtttat ggtgtttgac gtgatcagca gcgacgacgg cgtcaaaata acggcgtaat
     3301 cccaggtgcg ccagcaatgc ctcagcgatg gcgctttcac tccccgttcc tacagccatt
     3361 gggcgacgac catgccaact tttcaccact tcaacaagag gaagcggctc gacactatcc
     3421 agcagcatac ttcttactgc ttctgttttt tcacgcgcta acgcatgcgg gtcgagatcg
     3481 gcctgattca gctcaataat tgcctgagca atacgccagg tgggcgatcc attaagcgca
     3541 atcatcgcct gaatatcgta ctgaagaccg tagtgcccta atacttcgcg ccacgcttta
     3601 cggtgcgtag gcgccgtatc caggattgtg ccatccatat caaaaattaa acctgcataa
     3661 cgctcgtaca tggtcttctc gcaagtcgaa aaatatgacg ctactttagc gtaattgctt
     3721 gattttgtcg ctgatggcga aggagaagaa aattcaggaa atggataaag tagttattta
     3781 gaaatgagat atttttgaag gaaatttttt ggagaa
//
LOCUS       DADLTY010000276         3737 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.280, whole genome shotgun sequence.
ACCESSION   DADLTY010000276 DADLTY010000000
VERSION     DADLTY010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.280"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            368..2020
                     /locus_tag="JN334_004116"
     CDS             368..2020
                     /locus_tag="JN334_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY7440052.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2031..2201)
                     /locus_tag="JN334_004117"
                     /pseudo
     CDS             complement(2031..2201)
                     /locus_tag="JN334_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2280..2393)
                     /locus_tag="JN334_004118"
                     /pseudo
     CDS             complement(2280..2393)
                     /locus_tag="JN334_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989794.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2527..2853)
                     /locus_tag="JN334_004119"
     CDS             complement(2527..2853)
                     /locus_tag="JN334_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY7440053.1"
                     /translation="MPGASNAAMCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWI
                     EYYGKFWSRNFSYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYL
                     LRASNE"
     gene            complement(2918..3709)
                     /gene="ltrA"
                     /locus_tag="JN334_004120"
                     /pseudo
     CDS             complement(2918..3709)
                     /gene="ltrA"
                     /locus_tag="JN334_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137363.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1032 a          861 c          857 g          987 t
ORIGIN      
        1 atcggaaacg atgatctggc agcctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtccgtgaag ggaataaggc
      241 tcaggtcagc gaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt cagaacaacg gaagccttgc aaacggggat gcgttcatac
      361 aggaacaatg ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact
      421 gacaggaaat cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca
      481 tacaaatgac caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc
      541 ccaaaaaact ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct
      601 attattatct ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga
      661 acccgatatt cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga
      721 atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct
      781 ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt
      841 catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac
      901 aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag
      961 tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat
     1021 gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg
     1081 cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg
     1141 catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga
     1201 taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc
     1261 tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa
     1321 tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt acacccccga
     1381 aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc
     1441 acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt
     1501 tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga
     1561 cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga gtggtaacat
     1621 cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt
     1681 gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg
     1741 agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct
     1801 aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa catttagctt
     1861 taatgctact ggtggggatg gctatccacg tattgataat agacccggat acataaatac
     1921 cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac cgctggatgc
     1981 tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc
     2041 acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg tatgtttgcg
     2101 atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt gctgaattta
     2161 accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa
     2221 agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat tgatatttac
     2281 taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggtcagtcc
     2341 tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt cagacggcat
     2401 gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga accccgccgg
     2461 agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc gtcatacggc
     2521 tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct tcggatactg
     2581 tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt atttagactg
     2641 catccactta agcaaacgtg actgcattgc actccacagt cgatagctga agtttctgga
     2701 ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc gcctcgcaaa
     2761 atccagcaaa ctctcagctg ttgagcgatg tatacgccac ttcttgattg tttctgttat
     2821 tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt cgcctttgaa
     2881 attcttcaga gtacgcactt tgaagtcata tccgagagtg gcatacaaca tcatcggcgt
     2941 acctttcgaa gggaacgccg ggaaactgac gttgcatcca catatcaaag gcatagtgca
     3001 ggaacagatt tgagagcagg ggggagatta cgccgccctg cggcgttcct cgaccccgtg
     3061 ccagcattgc tccatccggc atttgcaccg gtgccttcag cctgcgctca atgtacatca
     3121 ccacccagcg tgcctgagta tgatgctgca ctgtcttcag tagcaggtca tgatcgatat
     3181 catcaacgaa gcctttaata tcgacttcca ccacccagtc aaacttccag cagcgtttcc
     3241 tcgcctgcgc aatcgcctgg tgtgcggatt tacccggtcg ataaccatac gagtctgcat
     3301 ggaacagggg ttccagtacc ggctcgagta tctgcttaac gaccatctgg gcgatccggt
     3361 ccgacacggc cggaataccg agcggacgta caccaccgtc agctttggga atgtccacac
     3421 gccttaccgc ctgcggcata taactgccgg aagccattct gttccacagt ttgtagagat
     3481 tgtctgtaac attctcgtca aacccggcca gtgtctgccc gtcaatgcct gccgccccac
     3541 ggttggcttt tactcgttta taagcttccc atacctgtcg tttacttaca ggaaatggct
     3601 ttgccgattt tacagcagtc ctcccatcgg cattgtcgcc cgtggttgcc gccgcaccat
     3661 cagcacaatg gggcaagccc tttgctccgg acccattacg gtccttcacc gctactacgg
     3721 tttactccgt ccctgta
//
LOCUS       DADLTY010000277         3719 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.281, whole genome shotgun sequence.
ACCESSION   DADLTY010000277 DADLTY010000000
VERSION     DADLTY010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.281"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..794
                     /locus_tag="JN334_004121"
     CDS             <1..794
                     /locus_tag="JN334_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7440054.1"
                     /translation="LPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDD
                     DNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFA
                     LIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQF
                     VVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYL
                     SGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            805..1896
                     /locus_tag="JN334_004122"
     CDS             805..1896
                     /locus_tag="JN334_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7440055.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="JN334_004123"
     CDS             complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="JN334_004123"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAY7440056.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2864..>3719
                     /locus_tag="JN334_004124"
     CDS             2864..>3719
                     /locus_tag="JN334_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY7440057.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKN"
BASE COUNT          951 a          955 c          919 g          894 t
ORIGIN      
        1 ggttaccgcg tagcctggtt tcctatcaga tgaccagcca gaaagatcgc ccaacccaac
       61 atgaaatgcg tcttgatggc tcactgctgg atgatggtcg cctgagctat agcctggaac
      121 aaagtctgga tgacgataac aaccataaca gtagcctgaa cgccagttac cgttcacctt
      181 atggaacctt cagtgccgga tacagttacg gtaatgacag cagccaatac aattacggcg
      241 ttaccggcgg cgtggttatc catcctcatg gcgtgacgct ctcgcaatat ctgggcaacg
      301 cttttgcgct tatcgatgct aacggggctt ctggcgtgag gatacaaaac tatccgggga
      361 ttgctactga tccctttggc tatgcagtgg ttccttatct cacgacttat caggaaaacc
      421 gtctctcggt agatactacg cagctgcccg ataacgtcga tcttgaacaa acaacacagt
      481 ttgtggtgcc caacagaggt gcaatggtag cggcgcgttt caacgccaat atcggttatc
      541 gcgtacttgt tacagtcagc gatcgcaacg gtaaaccgtt gccctttggc gctcttgcca
      601 gcaacgatga tacggggcaa caaagtatcg tcgatgaggg cggcatacta tatctctctg
      661 ggatatcgag taaatcacaa agctggactg tacgctgggg aaatcaggca gatcaacaat
      721 gtcagtttgc ttttagtaca ccggattcag aaccaacaac ctctgtatta caaggcacag
      781 cgcagtgcca ttaaggataa aaaaatgaaa agagcgcctc ttataacagg acttttgttg
      841 atatccacat cctgcgctta tgcctcctca ggagggtgtg gagccgacag cactagcggt
      901 gcgacaaatt acagcagtgt ggttgatgat gttacggtga accagacaga taacgtgaca
      961 ggacgggagt ttacctctgc aacgctaagt agcactaact ggcaatacgc ctgttcctgc
     1021 tctgcgggta aggcagttaa acttgtctat atggtcagcc ccgtacttac caccactgga
     1081 catcagacag gatattacaa actcaatgac agcctggata ttaaaaccac attacaggca
     1141 aacgacattc caggactcac aaccgaccag gttgtctctg ttaacacccg attcacacag
     1201 ataaaaagca gcaccgtata ttctgctgca acccaaacgg gtgtttgcca gggtgacacg
     1261 tctcgttatg gacccgttaa tattggtgca aataccacct ttaccctgta tgtcaccaag
     1321 ccatttctcg gctcgatgac cattccgaaa acggatattg ccgtcattaa aggcgcgtgg
     1381 gtcgatggaa tgggaagccc gtctacaggt gacttccatg atttagtcaa gttatcgatt
     1441 cagggaaatc tcaccgcccc acagtcgtgc aaaattaatc agggcgatgt tattaaagtt
     1501 aattttggat tcatcaatgg tcagaagttt accacccgca atgccatgcc agacggtttt
     1561 actccagtag actttgatat cacttatgac tgtggtgata cttcaaagat taaaaactcg
     1621 ttgcaaatgc gcatcgacgg tacaactggg gtagtagacc agtacaacct ggtcgccagg
     1681 cgaagaagtt cagacaatgt gcccgatgtc ggtattcgta ttgaaaatct cggcggcgga
     1741 gttgcaaata ttccttttca gaacggtatc cttcccgttg atccttccgg gcatggcacc
     1801 gtcaatatgc gcgcctggcc agttaatctg gtcggtggtg agctggaaac aggaaaattt
     1861 cagggcacag ccaccattac cgtcatggtg cggtaaaaga agacgttgcg ttttgattaa
     1921 cgcaacgctt cggcactgtt acccctgctg ctccagcgca tacttataca gcgcattttt
     1981 cttcacgcca tgaatttctg cggccagcgc cgccgctttt ttcagcggca gttctgcctg
     2041 tagcagcgcc agcgtgcgca gggcatcggc gggtaagtct tcttcctgtg ctttatgacc
     2101 ttcgacaatc agcaccattt cgcctttgcg acggttttca tcttccttta cccacgccag
     2161 cagctcgcca acgggcgcgc cgtgaatggt ttcccaggtt ttggtcagct cacgcgccag
     2221 aaccacgtag cgggattcgc ctaataccgc aacgatatct tccaggctat ctaacagacg
     2281 gtgggtagat tcataaaaaa tcagcgtgcg cggctccgct tcaatggctt ttagcgcatc
     2341 acggcggcct tttgatttgg caggtaaaaa gccttcgtaa cagaaacggt cagatggtaa
     2401 acccgcggcg cttaacgcag tgatagcagc acacggtccg ggtagcggca ccacacgaat
     2461 ccccgcttca cggcaggtac gcaccagatg gtagccagga tcgttaatta gcggcgttcc
     2521 ggcatcggaa accagcgcaa tgttttgccc ctcttgcagc tttgccagca gcgtttcggc
     2581 tttttgttgt tcgttatggt cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg
     2641 cagcaataaa ccggtgtgac gagtatcctc ggcggcaatc agatcaacgg cctgtaatac
     2701 ctctaacgta cgctgggtga tatccgccag attgccgatt ggcgtcggta caatgtaaag
     2761 ctggccctga gaattatccg ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat
     2821 ttaatattga gcattgcgta aaaaaaatat cactggatac attatggtac cctcaacatt
     2881 ttctcgtttg aaagccgcgc gttgtctgcc tgttgttctg gcagccctga ttttcgccgg
     2941 ttgtggcacc catactcccg atcagtccac tgcttatatg cagggcacgg cgcaggctga
     3001 ttctgccttt tatcttcagc agatgcagca aagctctgat gataccagga tcaactggca
     3061 attactcgcc attcgtgcac tggtgaaaga aggtaaaacc gggcaggcgg ttgagttgtt
     3121 taaccaacta ccgcaagaac tgaacgattc tcagcgtcgc gagaaaacac tgctggcggc
     3181 agagattaaa ctggcgcaga aagattttgc tggcgcgcaa aacttgctgg cgaaaatcac
     3241 acctgccgat ttagaacaaa accagcaagc gcgttactgg caggcaaaaa tcgatgccag
     3301 ccaggggcgt ccttccattg atttactgcg cgcgttaatt gctcaggaac cgctgcttgg
     3361 cgcgaaagaa aaacagcaga atattgatgc cacctggcag gcgctctcct ccatgactca
     3421 ggaacaggcg aatacgctgg tgatcaacgc cgacgaaaat attctgcaag gctggctgga
     3481 tctgcagcgc gtctggtttg ataaccgtaa cgatcccgac atgatgaaag ccgggatcgc
     3541 cgactggcag aaacgttatc cgaacaatcc gggcgcgaaa atgctgccaa cgcagttggt
     3601 taacgtaaaa gcgtttaaac cagcctcgac caacaaaatc gccctgctgt tgccgctgaa
     3661 tggccaggca gcggtatttg gtcgcactat tcagcaaggc tttgaagccg cgaaaaata
//
LOCUS       DADLTY010000278         3717 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.282, whole genome shotgun sequence.
ACCESSION   DADLTY010000278 DADLTY010000000
VERSION     DADLTY010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.282"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(2..382)
                     /gene="panD"
                     /locus_tag="JN334_004125"
     CDS             complement(2..382)
                     /gene="panD"
                     /locus_tag="JN334_004125"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAY7440058.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            656..1558
                     /locus_tag="JN334_004126"
     CDS             656..1558
                     /locus_tag="JN334_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY7440059.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            complement(1632..2483)
                     /gene="panC"
                     /locus_tag="JN334_004127"
     CDS             complement(1632..2483)
                     /gene="panC"
                     /locus_tag="JN334_004127"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAY7440060.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2495..3289)
                     /gene="panB"
                     /locus_tag="JN334_004128"
     CDS             complement(2495..3289)
                     /gene="panB"
                     /locus_tag="JN334_004128"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAY7440061.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            complement(3402..>3717)
                     /locus_tag="JN334_004129"
     CDS             complement(3402..>3717)
                     /locus_tag="JN334_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7440062.1"
                     /translation="GTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSN
                     SVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVT
                     YP"
BASE COUNT          895 a          913 c          960 g          949 t
ORIGIN      
        1 atcaagcaac ctgtaccgga atcgctttcg cggtacgttt catttcattg tcgccttcaa
       61 aataggcgac attgggtcgc caggtgcgag cttcttcatc tggcatggta acgaagctgg
      121 cgatgatgac aatatcgccg acgctggcgc agtgggccgc cgcaccgtta acagaaataa
      181 ttctcgaacc gcgttctgcc gcgatggcat aagtggagaa acgcttgccg ttggtgacat
      241 tccagatatc gatggcttcg ttttcgagaa taccggctgc gtcaagaaaa tcctggtcaa
      301 tggcgcaaga accttcatag tgcaggtccg catgagtcac tttcacgcgg tggagtttgc
      361 cctgcagcat cgtgcgaatc ataacttcta cctttctacc ctgtcgttaa cgaagcaggc
      421 ggtgcctgct ttgaggaaat tctcacgcag tattgcccga tttttgatga gtgtctactc
      481 atctgatggc atttgcgtca gcagtttgcg taccgcgccg aagcgtgcca tgtaacgcct
      541 gtaacgaatt gatattttta tgaacgctgc tcggtaaaca ataaagaggt ggctgacgat
      601 agcacgcctt gactggatac tgccttcacg caaggccaca cggaagacag ggaagatgga
      661 tgcaccgagt accacaccgc atgacgcggt atttaaacaa tttttaatgc atgcggagac
      721 ggctcgcgac tttctggaga tacatttgcc agtggaatta cgcgaacttt gtgatctcaa
      781 cacgcttcat ttagagtcgg ggagtttcat tgaagagtgc ctgaaaggtc acagcacgga
      841 cgtgctctat tccatgcaaa tgcagggcaa tcccggttat ctgcatgttg tgattgaaca
      901 ccaaagcaag ccggataaga aaatggcctt tcgcatgatg cgttattcta ttgctgccat
      961 gcaccggcat ctggaggctg gccacgataa gctgccgctg gtggtgccga tactgtttta
     1021 tcagggcgag gccacacctt atccgctctc aatgtgctgg tttgatatgt tttactcgcc
     1081 ggagctggcg cgacgcgtct ataacagtcc tttcccgctg gtggatatca ccatcacacc
     1141 ggatgacgaa atcatgcaac atcggcggat tgcgattctc gaactactgc aaaaacatat
     1201 tcgccagcgc gacttaatgt tattgctgga gcaactggtc acgctgattg acgaagggta
     1261 cactagcgga agtcagttag ttgccatgca aaactatatg ctgcaacgcg gtcatactga
     1321 acaagcggat ttgttttatg gtgtgctgag agacagggaa acgggaggga agtctatgat
     1381 gacgctggcg cagtggtttg aagagaaagg gattgagaag gggattcagc agggaagaca
     1441 ggaggaaagg caagaattcg ccctgcgtct tctaagtaaa ggaatgtctc gggaagacgt
     1501 tgcagagatg gcaaatttac ctcttgctga gattgataag gtaattaacc ttatttaagt
     1561 tacctgtgtt atgacagatg acgtggggta aattaataac tggcgccatc agccgtagcg
     1621 ccagttaagt attacgccag ctcgaccatt ttgttgtcga tcaggcgagc atcgccaagc
     1681 caggcggcta ccagaattac tgcccgtttg ctggtttcag aaacttccag caatgtgtcg
     1741 gcatcgcgaa tctgaatatc atcggcgcgg aagccttttt cattcagttc ttgccccgca
     1801 atggtaataa tttcatcgag atcccgttcc ccagcctgca atttgtcagc aatcgaactt
     1861 aaaactttgt acagacctgg cgcaattttg cgttgttccg ccgtcagata accgttacgg
     1921 gaacttagcg ccagaccgtc tttggcgcgc ataattggca caccgacaat ctcaatatcg
     1981 aagcccatat cggcaaccat tttgcggatc aacgccagtt gctgaaaatc tttttcaccg
     2041 aagcaggcga tgtccggctg gaccaggttg aacagcttgc tgacaatagt cgaaacgccg
     2101 cgaaaatgtc ccggacggct ggcaccttcc agcatggtcg aaaggccagg aacgtcaacg
     2161 taagtgtggg tttcagtacc gttcgggtag atctctttta ccgaaggggc gaaaactaaa
     2221 tccactttac gtttgtttag cttctcgcag tcctcctgca aggtccgtgg ataacgtgcc
     2281 agatcttccg ggcggtcgaa ctgcatcggg ttaacgaaaa tactgacgac gaccacatcg
     2341 gcgcgggctt tggcttcgtc gaccagcttc atatggccat tgtgcagatt acccatagta
     2401 ggcaccagcg ccacgcgctt gccttccata cgcaggcggc gaatttgctg acgcagcagc
     2461 ggcagggttt cgataattaa cacaacgtga ctccttaatg gaaactgtgt tcttcgcccg
     2521 gataaacgcc ggactccact tcagccatat actgccgcac agccgcgcgg atgtcgcccg
     2581 tttcggcgag gaaattttta gcgaatttag gaatgtgacc gccggtaata ccaaaggcgt
     2641 cgtgcatcac gaggatttgc ccgtcagtga cgttgcctgc accaatacca ataaccggga
     2701 tcgccagtgc ttcggtaata cgttttgcca gctcaaccgg cacgcattcc agcaccagca
     2761 gctgtgcccc cgcggcttct aaggctaatg catcgctgag tagtcgatcg ctcgcttcat
     2821 tgccgcgccc ctgtactttg tagccaccga aaatattcac tgactgtggg gttaaaccta
     2881 agtgaccaca tacaggaacg gcacgttcgg tcagcatttt tacggtttcg accagccact
     2941 caccgccctc aattttgacc atattggcac cggcacgcat aaccgttgcg gcgttttcaa
     3001 aggcttgttc cggcgtggca tacgccataa acggcaggtc agccaatagc aggcagtttg
     3061 gtgcgccgcg acgtacggcg gcagtgtggt aggcgatatc agcaacggtt actggcagag
     3121 tggaatcgtg gccctgaacg gtcatgccca gcgagtcgcc taccagcatg acgttaagcc
     3181 cttcatcagc aaatagtttc gcgaagctgt agtcgtaagc ggtgatggtc gcgaaacgct
     3241 ttttatcctg cttatatttc tgaagtgaag caatggtggt cggtttcata acgtatcctg
     3301 ataaatgatg tcgtgttgcc tggcatttta tcagtcacat tggtgggggc aatgatttat
     3361 caattaccgc accgccagat gatggcggtg cggtggactt attaagggta ggttacctgg
     3421 aaagtactgg tggctttaaa ctcaccagct tctattggta tagttccatc ctgttgtagc
     3481 gtggcctgga aatggagagg gtatgttata cctgtatctt gatccgggta aactcctcct
     3541 gttgtatcgt ctttgcctct cgggtcgtaa tctttataaa cagaatttga atcattaggt
     3601 ttcaatgtca ttagaggatt tttactagtt gctaaacctt ctatgagtac accgactcct
     3661 tttgctgcat cattacctgt gagagtatta ccaaggagtt gcccgttttc agtacca
//
LOCUS       DADLTY010000279         3687 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.283, whole genome shotgun sequence.
ACCESSION   DADLTY010000279 DADLTY010000000
VERSION     DADLTY010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.283"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..221
                     /locus_tag="JN334_004130"
     CDS             <1..221
                     /locus_tag="JN334_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HAY7440063.1"
                     /translation="QRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLPV
                     LQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            267..476
                     /gene="hha"
                     /locus_tag="JN334_004131"
     CDS             267..476
                     /gene="hha"
                     /locus_tag="JN334_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY7440064.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            515..1105
                     /locus_tag="JN334_004132"
     CDS             515..1105
                     /locus_tag="JN334_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY7440065.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1345..1605
                     /locus_tag="JN334_004133"
     CDS             1345..1605
                     /locus_tag="JN334_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HAY7440066.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1829..1903
                     /locus_tag="JN334_004134"
     CDS             1829..1903
                     /locus_tag="JN334_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HAY7440067.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1896..2753
                     /gene="repA"
                     /locus_tag="JN334_004135"
     CDS             1896..2753
                     /gene="repA"
                     /locus_tag="JN334_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAY7440068.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3454..3645
                     /locus_tag="JN334_004136"
     CDS             3454..3645
                     /locus_tag="JN334_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440069.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          997 a          902 c          928 g          860 t
ORIGIN      
        1 ttcagcgaga tcgttatggt cgtattctcg ggcaggttta tgcaccagac ggaatgaata
       61 ttaatcagtt tatggtccgg gccggggctg cctgggtata tgagcagtac aacacagatc
      121 ccgtgctacc tgtccttcag aacgaagcca gacaacaaaa acgtgggctc tggtcagatg
      181 ctgatccggt tcccccctgg atatggaggc accgtaaata aagcgatggc aatcctatca
      241 ttcatctgtt ctcagcggga ataaaaatgg caaaaacaaa acaggaatgg ctgtatcagt
      301 tacggcgatg ttcatcagtc aatacgctgg aaaaaattat tcataaaaac agagacagtt
      361 tatcaaacag tgaacgtgaa tcatttaatt cagcagcaga ccaccggcta gcggaattaa
      421 tcaccggaaa gttatatgac aggataccaa aagagatatg gaaatatgta aggtaataat
      481 tcagcaataa ttaattgcca ggagcagata aataatgaag ttgattattt ttattctcat
      541 tgttctgatt attgcagcgc tacttatccg gattatcctc agaagtgtaa atcagcattc
      601 tcctttgctg atgcaattgc atgcggcagg tatacggact ggtgatgcag agcgaatact
      661 gtccagcggt gaatactggc aacgtcagaa gacgctgctg acagaaaggg aagtcagttt
      721 tatgaaagga ctgttcagaa ttgtggatat gaagcggtgg tatctgtgtc cgcaggtacg
      781 ggtagcggat atcgtccagc tgaacgggaa tatccggcca cgatcacgcc agtggtggca
      841 gttattcagg atggtgtctc agtggcatgt tgatgtggtt atcgttgagc tgcgttcgtt
      901 cagtattgtt gcagcagtag agctggatga tgccagccat ttacgaccgg aacgcagacg
      961 ccgggatatt cttctggaag aggttctgag gcaggctggt attccgttgc tcagaagcca
     1021 cgatgccaga aaactgctgc agatgaccgg agaatggctg aatacaacag gggctgatca
     1081 gcagtcaccg gaacatcgta gctgacgcct tcgcgttgct cagttgtcca accccggaaa
     1141 cgggaaaaag caagttttcc tcgctcccgg cgtttccata actgaaaacc atactatttc
     1201 acagtttaaa tcacattaaa cgacagtaat ccccgttgat ttgtgcgcca acacagatct
     1261 tcgccacaat tctcaagtcg ctgatttcaa aaaactgtag tatcctctgc gaaacgatcc
     1321 ctgtttgagt attgaggagg cgagatgtcg cagacagaaa atgcagtgac ttcctcattg
     1381 agtcaaaagc ggtttgtgcg cagaggtaag cctatgactg actctgagaa acaaatggcc
     1441 gctgttgcaa gaaaacgtct tacacacaaa gagataaaag tttttgtcaa aaatcctctg
     1501 aaagatctca tggttgagta ctgcgagaga gaggggataa cacaggctca gttcgttgag
     1561 aaaatcatca aagatgaact gcagagactg gatatactaa agtaaagact ttactttgtg
     1621 gcgtagcatg ctagattact gatcgtttaa ggaattttat ggctggccac gccataaggt
     1681 ggcagggaac tggttctgat gtggatgtac aggagccaga aaagcaaaaa ccccgataat
     1741 cttctttaac tttggcgagt cagaaagatt accggggccc acttaaaccg tatagccaac
     1801 aatcaagcta tgcggggagt atagttatat gcccggaaaa gttcaagact tctttctgtg
     1861 ctcgctcctt ctgcgcattg taagtgcagg atggtgtgac tgatcttcaa caaacttatt
     1921 accgccaggt aaagaacccg aatccggtgt tcacaccccg tgaaggtgcc cggacgcttc
     1981 cgttctgcgg gaaactgatg gaaaaggcgg tgggcttcac ctcccgtttt gatttcgcca
     2041 ttcatgtggc acatgcccgt tcgcttggtc tgcgtcggcg catgccaccg gtactacgtc
     2101 gccgggccat tgatgcgctg ctgcaggggc tgtgttttca ctatgaccca ctggccaacc
     2161 gcgtccagtg ttccatcacc acgctggcca ttgagtgcgg actggcgacg gagtctgctg
     2221 ccggaaaact ctccatcacc cgggccaccc gggccctgac gttccttgca gagctgggac
     2281 tgattaccta ccagacggaa tatgacccgc ttatcgggtg ctacattccg accgatatca
     2341 cgttcacatc tgcactgttt gctgccctcg atgtatcaga ggaggcagtg gccgccgcgc
     2401 gccgcagtcg tgtggaatgg gaaaacagac agcgcaaaaa gcaggggctg gataccctgg
     2461 gtatggatga actgatggcg aaagcctggc gttttgttcg tgagcgtttc cgcagttacc
     2521 agacagagct taagtcccgt ggaatgaagc gtgcccgtgc gcgtcgtgat gcggacaggc
     2581 agcgtcagga tattgtcacc ctggtgaaac ggcaactgac gcgtgaaatc tcggaagggc
     2641 gcttcactgc cagtcgtgag gcggtaaaac gtgaagtgga gcgtcgtgtg aaggagcgca
     2701 tgattctgtc acgtaaccgt aattacagcc ggctggccac agcttccccc tgaaagtgac
     2761 ctcctcagaa taatccggca cgcgccggag gcattcgcac gcctgaagcc cgccagtgca
     2821 caaaaaatca gcgctacata caaaaaacaa cctcatcatc caccttcagg tgcatccggt
     2881 cccccctgtt tttgatacaa aacacgcctc acagacgggg aattttgctt atccacatta
     2941 aactgcaagg gacttcacca taaggttaca accgttcacg tcataaagcg ccagacgtca
     3001 accctacagg gtgcaatgta tcttttaaac acctgtttat atctccttta aactacttaa
     3061 ttacattcat ttaaaaagaa aacctgttca ctgcctgtcc tgtggacaga caaatatgca
     3121 cctcccaccg caagcggcgg gcccccaacc gaagccggtt tagttacaac atgcaaacat
     3181 aaacccctaa aaaacccaag tccaacgcaa aactgaaacc acaacgcccc cctcataact
     3241 gaaaagcgcc cccgcccccg cccaaagggc gggaacagag tcgcttttaa ttatgaatgt
     3301 tgtaactaca tcatcatcgc tgtcagtctt ctggctggac gtatcgaata cacgctcgta
     3361 agcggccctc acggcccgct aacgcggaga tacgccccga cttcgggtaa aaccctcgtc
     3421 gggaccactc cgaccgcgca cagaagctct atcatggctg aaaacgggta tggcttggca
     3481 gggctgggga tgggtaaggt gaaatctgtc aatcagtacc ggctgacgcc gggcttcggc
     3541 ggttttactc cggtatcaca tgtaaccaca gcgtgccgcc ttccatgccg ctggcgcggc
     3601 atcagaataa tacaagcagc atttaatgca tttgcaaaag tgtgagtaat attaagtggt
     3661 cgtttcaggg atatcgttaa gacttga
//
LOCUS       DADLTY010000280         3634 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.284, whole genome shotgun sequence.
ACCESSION   DADLTY010000280 DADLTY010000000
VERSION     DADLTY010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.284"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..1393)
                     /gene="tssF"
                     /locus_tag="JN334_004137"
     CDS             complement(<1..1393)
                     /gene="tssF"
                     /locus_tag="JN334_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HAY7440070.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLT"
     gene            complement(1409..1903)
                     /gene="tssE"
                     /locus_tag="JN334_004138"
     CDS             complement(1409..1903)
                     /gene="tssE"
                     /locus_tag="JN334_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HAY7440071.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(1900..2730)
                     /locus_tag="JN334_004139"
                     /pseudo
     CDS             complement(1900..2730)
                     /locus_tag="JN334_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(2717..3631)
                     /gene="tagK"
                     /locus_tag="JN334_004140"
                     /pseudo
     CDS             complement(2717..3631)
                     /gene="tagK"
                     /locus_tag="JN334_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT          771 a          960 c         1074 g          829 t
ORIGIN      
        1 cggttagatc gtcccggcca ttgcgggcaa taagacaggg cagatcgcgg ttagtgacca
       61 tggccgtgat ggtgatgtgg cgcagatttt ccgggtacgg cgcttcatac tggtcaacca
      121 gcgacaggaa cacttccgag cccgtatagg gggtacgggt gccgtaacgg cgggcgtttt
      181 ctgataagcg gcggggctcc cggcgcaggg aaaaataacg tccgtgattg ccttcatcat
      241 tattgcgggt gtggtagagc gggcgaaagg ccatcttccg tgtggtgtcg gtttccagcc
      301 cttccacttc ctggaccgag aacacctcat aatccagcgg atgggttctg tccaccacca
      361 gatgctgttc cgtcgtgctg tgcgtgacat cgatccgggc ggtgacgcgg ggaaacaggt
      421 tgataaccgg ggtacagaac aggctgaact gtgccgcatt tgtctggtgg atcagccagt
      481 ccggcggcag gcggttcagc aggataacaa tctccgccac gccaccgtca attttctgta
      541 atccggcaga caggcctgtc ggggtgaaaa aatagaagcg ttccggacag gcaaaatatt
      601 catgcagcag gttatgaccg tgaaacacgt tccacgccag cggcagcagc ccctgatccg
      661 gctccaggcc ttcgtacata acgggctgtt gcagattcac atccagcgcg ccgtcaaaat
      721 gccccggaat gccagccagg ggcgcgatgg cgctggtatg tagaagttct aacaggtgtg
      781 aggccgtacg ctcctcgccg cagagataaa acggcagcct gtccagtccg gcaagctgac
      841 tgaacgttag ttcaccaaag gtgcgcaggg taatacgcag cgccccggcg acgtgaatgt
      901 tggccggcag gtagcggtgc agggcaggca tatccggtgg cgcggcggtc agtcgcgcct
      961 cttcgatggc gagcggccac agcgttacat cctggctgct gcgaaactgg caggcagtgc
     1021 tttcgccttc cggaatggcg gagaaaaagg ctgtgtcgcg cgggacggta aatcctttcg
     1081 ccagatcgcc ttcttccgta tcgggatgca actgcgccac gctcattgag ggcgtgggtg
     1141 tgacgtaatt ggggctgacc acttccagca ggcgctgggt gaaacggggg aattcagcgt
     1201 caatttttag ctgcgtgcgg gcggtcagga agctgaaggc ctcgaccatt cgctctacat
     1261 acggatcggc aatatccgtt ccctgcatgc ccagccgggc ggcgactttg ggatgacggg
     1321 cggcaaattc ggctcccgtt tcccgcagat agctcagttc gcggttgtag tattccagta
     1381 gccgtggatc catcggtctc tctctgaatt aaaaaaacgt aatgcgactc tgctccatat
     1441 ccagcgcgct acgtacccgg aatgcagtcg gatacggctg cgtgaggatc tcgccgcgaa
     1501 tttcaaactg taatgtgtta tggccactct ggcggcgctc atcctgaagt gggatgattt
     1561 gcagagtgga cgctttcagc cggggttcaa accgggtgat ggttcgcaaa atggccctgc
     1621 ggatatcgtc ccacttgtgc tcatacagaa agctgcccgc cagtggcggc aggccgtagt
     1681 taagtacgga cgctgccgcg tacggataac gtgcagcatc aatatcatct tcatggctga
     1741 cggtgttgag cagaaatgac aaatcccggc ggatgatctc cttaatctga accggactga
     1801 cgctgatacc gtggtcgcgt ttctggtttg gggcgtcgtc acacaggcgg tcaaacagcg
     1861 tgggcagcag gtgattgcct ggacgctggc gggaagcgct cacgcgtcgc ttccttcagc
     1921 ggtgttaaag gtacaggtgg acaaatccag caggctgata tcgccgtgac cggtgagcca
     1981 caccctctga cccagcgcct gtaccagtgt ctctcccgga ccgtcctgcc agaccgtttc
     2041 ccggcacaga cgcagggagt cggcagcctt ttcagagccg ctgtagcggg gaaacagcta
     2101 ggcactgtgt gtatcgccat tgttcagggt gacgttgacg ggtttccaga tcaaatcgat
     2161 cagatgcgca ggccgcggcg atttcagcga acagatttgt gaaaacggca gccagatata
     2221 tgctccgccc gcgatcagtt cgagtaccgg accggtacgg gagtcgctgt cgctaatcca
     2281 gtcaaatgcg ccgccattcc actgcccgga agtgtctgtt atggcctcaa gcgcgcggct
     2341 gcggtgagca tcagcttcct gcgcttcacc acgggcgtta caggcgaggg cggtcagcag
     2401 atcctccatc caggcgggcg gcgggagaat aacaccgggc cgctgttcgc cctgaaagca
     2461 ggcgtggcgg tatatctcac agcggatcag ctcgccaaat acctgcgctt cgcgagtata
     2521 atttgcgtcc atccgggcgc atagctggat ctgttgcagt gcccgcgacc agtcgcctgc
     2581 cacgcacaat aactgaaaca ggctgtggcg gcgggaggcc tgcgctgggt gcatcctgac
     2641 ctgttgctct gccatctgaa tgccctccac cacggaataa tcccgcagca gcgccgacaa
     2701 ggtaatcggt agctcgttat tgtttgtcat gatatatctc tccatgttgt gccgggttta
     2761 tccggtaatg gctgtcaatg ccaatgatgc gatgctcttt ccgtgtcaga tctggcagta
     2821 cggtgttatg cgctgtttcc ggggcgtatt ccggggaaag taaatgcagg atatcagggg
     2881 gagtcttttc gacaggccag atagtttcat cttcgccagt ggcatccagt acattgagaa
     2941 tagcgtcaat gcttaaccca ccagtgacca tatcctgcaa tgtctcggct tcggtgacca
     3001 ggtcacgcaa tgaatccaga tcctgcgagg agggctcttc gttaaaagga aaaggatcag
     3061 catggcgatt aagatcttgt tcgggagata acaacgcctc gcggtattcc tgataaagtc
     3121 gggtcagggc atttcctttt tccgcacctt ctccggtgga ggaggctcca tgaacaggaa
     3181 tgatatcgaa tggattttgt gatgtcagat gctgctgcct gaaccaatcc agatccagat
     3241 attcggttat tgcttccgac gtgatggcag tctgcgatat cgtttttact gtgctcaggt
     3301 tcggagatga tggattgcca tgtacaaggc actatgaaga taacccccat tctatcaggt
     3361 cgccttcatt cagacgcata cggtgatgag gttccacaat ctggtcgttt accgtacagc
     3421 aatattcggt agactgattg ataatccacc atgcttcttc gtggcgggtc agagtgaggg
     3481 caatctttac atcatcattc accttaaaaa caggaatcca gggagaggta acggtgaaga
     3541 taatttgtgt tccttcagtc gcgtaaattg tgttatcggc gatcccgtgc gcctgtattt
     3601 tttggagtcc ccacgtaata tttggtttca ttgt
//
LOCUS       DADLTY010000281         3548 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.285, whole genome shotgun sequence.
ACCESSION   DADLTY010000281 DADLTY010000000
VERSION     DADLTY010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.285"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..465
                     /locus_tag="JN334_004141"
     CDS             <1..465
                     /locus_tag="JN334_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7440072.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /locus_tag="JN334_004142"
     CDS             802..2415
                     /locus_tag="JN334_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7440073.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="JN334_004143"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="JN334_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HAY7440074.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          819 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DADLTY010000282         3537 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.286, whole genome shotgun sequence.
ACCESSION   DADLTY010000282 DADLTY010000000
VERSION     DADLTY010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.286"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..909
                     /gene="selB"
                     /locus_tag="JN334_004144"
     CDS             <1..909
                     /gene="selB"
                     /locus_tag="JN334_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAY7440075.1"
                     /translation="LWLADNDRLVLRDISARNTLAGARVVMLNPPRRGKRKPEYLQWL
                     ASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGEGMRELLQQPGYIQAGYSLLNAP
                     VAARWQRKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLLLIEKMRESGDIL
                     SHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWWVRDLAKETGTDEQAMRLTLRQA
                     AQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGSTCAADFRDRLGVGRKLAIQILE
                     YFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            1099..2250
                     /gene="yiaY"
                     /locus_tag="JN334_004145"
     CDS             1099..2250
                     /gene="yiaY"
                     /locus_tag="JN334_004145"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HAY7440076.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            2415..>3537
                     /locus_tag="JN334_004146"
     CDS             2415..>3537
                     /locus_tag="JN334_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY7440077.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLT"
BASE COUNT          874 a          863 c          945 g          855 t
ORIGIN      
        1 ttatggctgg cagataacga ccgcctggta ttgcgcgata tctctgcccg caacacgctg
       61 gccggagcgc gcgtcgtgat gcttaacccg ccgcgtcgcg gtaaacgtaa accagagtat
      121 ctgcaatggc tggcttccct cgccagggca cagaatgata gcgaagcgtt agccgttcac
      181 ctgcaacgcg gcgcggttaa tcttcctgat ttcgcttggg cgcgccagct caacggcgaa
      241 gggatgcgcg aactgctgca acagcctggc tatattcagg ctggttatag cttgttgaat
      301 gcgccggttg ccgctcgctg gcaacggaaa attctcgaca cattagcgac ttatcatgag
      361 caacatcgcg atgaacctgg gcctgggcgc gaacgtctgc gacgtatggc gttgccaatg
      421 gaagatgaag cgctagtact gttgctgatt gaaaagatgc gcgaaagcgg cgacatcctc
      481 agtcatcacg gttggctgca tctgccggat cacaaagcgg gcttcagcga agagcagcag
      541 gccatctggc aaaaagcaga gccgctgttt ggtgacgagc cgtggtgggt gcgtgatctg
      601 gcaaaagaga cgggtaccga cgagcaggca atgcgcctga cgctacgcca ggcggcgcag
      661 caaggaataa ttaccgcgat cgtcaaagat cgttattacc gtaacgatcg gattgtcgaa
      721 tttgccaata tgatccgcga tctcgatcag gagtgtggtt caacctgcgc ggcggatttc
      781 cgcgatcgct taggcgtagg ccgaaagctg gcaattcaga ttctggaata ttttgaccgc
      841 attggcttta cgcgtcgtcg tggaaatgat catttattgc gcgacgcatt attatttccg
      901 gaaaaataag gaaatgatta attaattttt aaaataaatt aatacaaatt tcttatgatt
      961 ttaaataaag cacattgttt aatgaataca atgtgctttt tattagatta attttgataa
     1021 ttaataataa tgatcatgtt ttcgaatgaa aatcctcagt aagctgccca ccctttttta
     1081 cactttcagg agtgtgttat ggcatcttca actttcttta ttccttctgt gaatgtcatc
     1141 ggcgctgatt cattgactga tgcaatgaat atgatggcag attatggatt tacccgtacc
     1201 ttaattgtca ctgacaatat gttaacgaaa ttaggtatgg cgggtgatgt gcaaaaagca
     1261 ctggaagaac gcaatatttt tagcgttatt tatgatggca cccaacctaa ccccaccacg
     1321 gaaaacgtcg ccgcaggttt gaaattactt aaagaaaata attgcgatag cgtgatctcc
     1381 ttaggcggtg gttctccaca cgactgcgca aaaggtattg cgctggtggc agccaatggc
     1441 ggcgatattc gcgattacga aggcgttgac cgctctgcaa aaccgcagct gccgatgatc
     1501 gccatcaata ccacggcggg tacggcctct gaaatgaccc gtttctgcat catcactgac
     1561 gaagcgcgtc ataacaaaat ggcgattgtt gataagcacg tgactccgct gctttctgtc
     1621 aatgactcct ctctgatgat tggtatgccg aagtcactga ccgccgcaac gggtatggat
     1681 gccttaacgc acgctatcga agcgtatgtt tctattgccg ccacgccgat cactgacgct
     1741 tgtgcactga aagccgtgac catgattgcc gaaaacctgc cgttagccgt tgaagatggc
     1801 agtaatgcga aagcgcgtga agcaatggct tatgcccagt tccttgccgg tatggcgttc
     1861 aataatgctt ctctgggtta tgttcatgcg atggcgcacc agctgggcgg tttctacaac
     1921 ctgccacacg gtgtatgtaa cgccgttctg ctgccgcatg ttcaggtatt caacagcaaa
     1981 gtcgccgctg cacgtctgcg tgactgtgcc gctgcaatgg gcgtgaacgt gacaggtaaa
     2041 aacgacgcgg aaggtgctga agcctgcatt aacgccatcc gtgaactggc gaagaaagtg
     2101 gatatcccgg caggcctacg cgacctgaac gtgaaagaag aagatttcgc ggttctggcg
     2161 actaatgccc tgaaagatgc ctgtggcttt actaacccga tccaggcaac tcacgaagaa
     2221 attgtggtga tttatcgcgc cgcaatgtaa tcatcattcc aacaacggct ggcaaattgt
     2281 cagccgcttt ttcaactatc tctgtaatcc ttgcccgtaa attcgtgata gctgtcgtaa
     2341 agctgttacc gactggcgaa gatttcgcca gtcacgtcta cccttgttat acctcacacc
     2401 gcaaggagac gatcatgacc aataatcccc cttcagcaca gattaagccc ggcgagtatg
     2461 gtttccccat caagttaaaa gcccgctacg acaactttat tggcggtgaa tgggtagccc
     2521 ctgccgatgg tgagtattac cagaatctga cgccggtgac cgggcagctg ctgtgcgaag
     2581 tggcgtcttc gggcaaacga gacatcgatc tggcgctgga tgctgcgcac aaagtgaaag
     2641 ataaatgggc gcacacctcg gtgcaggatc gcgcggcgat tctgtttaag attgccgatc
     2701 gaatggaaca aaacctcgag ctgttagcga cagctgaaac ctgggataac ggcaaaccca
     2761 ttcgcgaaac cagtgctgcg gatgtaccgc tggcgattga ccatttccgc tatttcgcct
     2821 cgtgtattcg ggcgcaggaa ggtgggatca gtgaagttga tagcgaaacc gtggcctatc
     2881 atttccatga accgttaggc gtggtggggc agattatccc gtggaacttc ccgctgctga
     2941 tggcgagctg gaaaatggct cccgcgctgg cggcgggcaa ctgtgtggtg ctgaaacccg
     3001 cacgtcttac cccgctttct gtactgctgc taatggaaat tgtcggtgat ttactgccgc
     3061 cgggcgtggt gaacgtggtc aatggcgcag gtggggaaat tggcgaatat ctggcgacct
     3121 cgaaacgcat cgccaaagtg gcgtttaccg gctcaacgga agtgggccaa caaattatgc
     3181 aatacgcaac gcaaaacatt attccggtga cgctggagtt gggcggtaag tcgccaaata
     3241 tcttctttgc tgatgtgatg gatgaagaag atgccttttt cgataaagcg ctggaaggct
     3301 ttgcactgtt tgcctttaac cagggcgaag tttgcacctg tccgagtcgt gccttagtgc
     3361 aggagtcaat ctacgaacgc tttatggaac gcgccatccg ccgtgtcgaa agcattcgca
     3421 gcggtaaccc gctcgacagc gtgacgcaaa tgggcgcgca ggtttctcac gggcaactgg
     3481 aaaccatcct caactacatt gatatcggta aaaaagaggg cgctgacgtg ctcacag
//
LOCUS       DADLTY010000283         3510 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.287, whole genome shotgun sequence.
ACCESSION   DADLTY010000283 DADLTY010000000
VERSION     DADLTY010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.287"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            1..452
                     /locus_tag="JN334_004147"
                     /pseudo
     CDS             1..452
                     /locus_tag="JN334_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            512..619
                     /locus_tag="JN334_004148"
     CDS             512..619
                     /locus_tag="JN334_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HAY7440078.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(600..1331)
                     /gene="yehW"
                     /locus_tag="JN334_004149"
     CDS             complement(600..1331)
                     /gene="yehW"
                     /locus_tag="JN334_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HAY7440079.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1336..2262)
                     /gene="yehX"
                     /locus_tag="JN334_004150"
     CDS             complement(1336..2262)
                     /gene="yehX"
                     /locus_tag="JN334_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HAY7440080.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(2255..3412)
                     /gene="yehY"
                     /locus_tag="JN334_004151"
     CDS             complement(2255..3412)
                     /gene="yehY"
                     /locus_tag="JN334_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HAY7440081.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3419..>3510)
                     /locus_tag="JN334_004152"
     CDS             complement(3419..>3510)
                     /locus_tag="JN334_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine/betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY7440082.1"
                     /translation="QQLNASIAVEGLDAKKVAADYLKQKGWAK"
BASE COUNT          879 a         1082 c          871 g          678 t
ORIGIN      
        1 caatctacat agcctgcgaa cgtggatcaa agagcgcggt caggattacc ccgcccagac
       61 actcaccaca catctgttta ttcctctgcg ccgacggctt cagtgccaac aaccgactct
      121 ccaggcgctg ctggcgatcc tcgacggcgt actgatcaac tacatcgcca tttgtctggc
      181 ttcggcacgt aaaaaacagg gtaaagatgc gctggtggtt ggctggaata ttcaggatac
      241 cacccgtctg tggctggagg gctggattgc cagtcaacaa ggatggcgca ttgatgtcct
      301 cgctcgccca ctcgctcaat caactacgcc ctgaactatt cgaaggccgt acattgctgg
      361 tgtggtgcgg tgaaaatcga acctccgccc aacagcagca actcaccagt tggcaagaac
      421 aaggccatga tattttccca ctcggtattt aatgattcgt taacaaatgc gctttactgt
      481 acaatccttt cgttaacata aggagtgcat tatgcgcata gctaaaattg gggtcatcgc
      541 cctgttcctg tttatggcgt taggcggaat tggtggcgtc atgctcgcag gttatacctt
      601 tattttgcgt gctggctaag cgcctgcacc agcctttcaa acaggcggtc tgcgatgatc
      661 gccgccagtg ccaccagtaa cgccccctgg atcacatacg cggtattaaa tccgctaagc
      721 ccgatgatga tgggcgtacc cagcgtactg gcccctaccg ttgaggcgat cgtcgccgta
      781 ccaatgttga taatcaccga agttcgcacg cccgccagaa tcaccggagc cgccagcggt
      841 agctcgacct tacgcagtcg ctgacctcga ctcattccca tacctttcgc aacttctgtc
      901 acgctgggat caatcgctcc cagcccggca agtgtcacct gcaggatggg cagcacaccg
      961 taaaggatca aggcgataat cgctggttgc agaccaaagc cgatcaccgg aacggcaatc
     1021 gccagcactg cgacaggcgg aaaagtctgt ccaacggcgg caatagtttc caccagtggg
     1081 cgaaattccg cgccccacgg gcgagtaaca gcaattccgg cacctgtgcc aatgatcacc
     1141 gcaaacaaac tcgaaattcc caccagccag aaatgagcca gtgccagagc tgcaaaactt
     1201 tcttgctgat aaacgggtcg tggcagttgt gggaacaagg cagcaaacag cggctggctg
     1261 taaggcagcc agaaaatcag cgccacaaaa agagcaatga gccagaacag cggatcgcgc
     1321 aacatcttca tacgcttacg cctccaccag cagatcctga aaatgcagcg tgccgcaagg
     1381 ctggccctgc gtgttcacca ccggcagcac ctcgcatccc cgcgcgacaa acagggagag
     1441 cgcatcgcgt agcgtcatct cttctgccag tgcctcacct tctgcccgtt cttcgcgacg
     1501 cacgtaatcc gccacgctac gtaacgaaag caggtgcacc cccagttcac tacgtccaaa
     1561 aaactggcgg acaaaatcat tcgccggacg agtcagcatc gtcagcggat tcccctgctg
     1621 caccacttca ccgtgatcca tcaataccag atgttctgcc agccgtagcg cctcatcaat
     1681 atcatgagtg accagcacaa tagtgcgccc cagcaaacgg tgaatgcgcg tcatctcttg
     1741 ttgcaacgcg ccgcgcgtta ccgggtccag tgcgccaaaa ggttcatcca tcagtaagac
     1801 ttgcggatcg gcagccagtg cacgcgccac tcccacacgt tgctgctgac caccggaaag
     1861 ctgatgcgga taacgctcac gcaaatttga ctccagcccc agtagcgcca ttaattcgtc
     1921 gatacgatcg tcaatccgcg cccgcgacca tttttgtaat tgcggcacgg tagcgatgtt
     1981 ttgcgccacg ctccagtggg ggaacaggcc aatagattga atggcatagc ccatccggcg
     2041 gcgcaactcc agtactggca gcgagcgaat ttcttctccg gcaaagcgga tcactccgct
     2101 gtcatgctcc accaggcggt taatcatttt cagggtggtg gatttgccgg agccagatgt
     2161 gccaatcagc accgaaaaac tcccttcctg aaaattgaga ttgagatcgt taacggcttt
     2221 ttgtgcgccg aacagtttgc tgacatggct aaattcaatc attacgtttc accttcagca
     2281 gtgcgataag caaatcgaac agcgcgtcgg taagcaccgc cagaacaatt accgggatca
     2341 cccccagcaa cactaaatca atggcgctgc ttagcagccc ctggaaaacc agcgcaccaa
     2401 aaccgcctgc gccgattaac gccgcaatca ccgccatacc tacagtttgc accatcacca
     2461 cccgcaggct gcgcagaaat accggtaacg ccagcggtaa ctgaacatgc aggaaacgct
     2521 gcgccccgct catccccatc gctctggcgc tctccagcac atcgcgcggg atctggttca
     2581 aaccgactac cacgcctcgc accagcggca gcaaggcata gagcaccagc gcaatcagtg
     2641 cgggtgtcat tccggttcct gctatgccga gcttccccag ccacggaaag gccgtaacca
     2701 gcgcggcaag cggcgcaatc aacaggccaa agagcgccac cgaaggcacg gtctgaatga
     2761 cattgagcag agaaaaaatt gccccctgcc gcgcagtgga aaagtagcac cagatgccca
     2821 acggcacacc aatcactaac gcaggcagca ccgcaccaaa cagcaacgtc agatgttgtg
     2881 ccagcgcgtc gtcaaacaca tcctgacggt tggcgtattc tttcattagt gagagatcgt
     2941 taagcgtgcc ggagtacagc aaccacagcg gaataatggc aatctgcata tgcaacaacc
     3001 agcgccacag cggatgcgtg gagattcggc ggatggcatc gctacaggcc agcaatgcca
     3061 gcgccgcagc cagccagaaa cctctgccga ggctggtacg cgccagcgca ctgccatttt
     3121 gcgccagttg ggtcgccgcc tttccagctc cccacaccaa caatacgaag acgaattgcg
     3181 ccagaatgag tgcaaaaatg ctgccttttt tagcggggac aaaacaggct gtcagccagg
     3241 cgcaaccaac gcccaccagc atccagatcg tttgcggcca cagttgccag agatgaagcc
     3301 cttcacccga gactaaacga ttaggcgcgt aactgataaa cggcagcgcc gctgcgattg
     3361 ccgtcagcaa caacagcagc gccagaacag gattaatacg tagataagtc acgggaaatt
     3421 acttcgccca ccctttttgt ttcaggtagt cggcagccac ttttttggca tccagtcctt
     3481 ccactgcaat gctggcattc agttgctgca
//
LOCUS       DADLTY010000284         3468 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.288, whole genome shotgun sequence.
ACCESSION   DADLTY010000284 DADLTY010000000
VERSION     DADLTY010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.288"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            1..1985
                     /locus_tag="JN334_004153"
                     /pseudo
     CDS             1..1985
                     /locus_tag="JN334_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
     gene            1991..2704
                     /gene="ybiX"
                     /locus_tag="JN334_004154"
     CDS             1991..2704
                     /gene="ybiX"
                     /locus_tag="JN334_004154"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HAY7440083.1"
                     /translation="MWGAGPTFWRTCMMYHIPGVLSPQDVAHFREQLEQAEWVDGRVT
                     TGAQGAQVKNNQQVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNN
                     ETYGFHVDGAVRSHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGD
                     LVLYPSSSLHCVTPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENE
                     EILSLLNLYHNLLREWSEI"
     gene            2778..3044
                     /gene="ybiI"
                     /locus_tag="JN334_004155"
     CDS             2778..3044
                     /gene="ybiI"
                     /locus_tag="JN334_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HAY7440084.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            3309..>3468
                     /locus_tag="JN334_004156"
     CDS             3309..>3468
                     /locus_tag="JN334_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440085.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEA"
BASE COUNT          895 a          923 c          870 g          780 t
ORIGIN      
        1 gcgtgggtgc gttttttgcg ggtgagaacg gtaactccac cactggcgac gccatttata
       61 tgcgtggtgc cgatacctct aacagtattt atattgatgg tattcgcgat atcggcagcg
      121 tctcgcgcga caccttcaat accgagcagg tcgaagtgat taaagggccg tccggcaccg
      181 actacgggcg cagcgcaccg acaggctcga tcaatatgat cagcaagcag ccgcgcaatg
      241 attccggcat tgacgcctcc gccagtattg gcagcgcctg gttccgccgc ggcacgctgg
      301 acgtcaatca ggtcattggt gatactaccg cggtgcgcct gaatgtgatg ggcgaaaaaa
      361 cccacgatgc cggacgcgac aaagtcaaaa atgagcgtta cggcgtcgcc ccttctgtcg
      421 cttttggcct tggtacagca aatcgtttgt atcttaatta tctgcatgtc actcagcaca
      481 acacgccaga cggcggcatt ccgaccatcg gtttgccggg ctattctgcc ccatctgcgg
      541 gagcggcggc cctgaatcat tccggaaaag ttgatactca taacttttac ggcacggatt
      601 ccgattacga cgattcgacc accgacaccg ccaccatgcg ttttgagcac gacatcaacg
      661 ataacaccac cattcgcaat actacccgtt ggtcgcgcgt aaagcaggat tacctgatga
      721 cggcgattat gggcggggcg tcgaatatta ctcagcccac cagcgatgtg aatagctgga
      781 cctagtcacg cacggcgaat accaaagatg tgagtaataa aattctcacc aaccagacca
      841 acctgacctc gacgttctat accggttcta tcggtcatga tgtcagtacc ggcgtggaat
      901 ttacccgtga aacgcagact aactatggcg ttaatccggt gacgttaccg gcggtaaata
      961 tttatcatcc tgacagcaga attcatcccg gcggcctgac gcgcaacggc gcaaacgcca
     1021 atggtcagac ggataccttc gcaatttacg ccttcgatac gctgcaaatc acccgtgatt
     1081 ttgagctgaa cggcgggatc cgtctggata attatcatac tgaatatgac agtgccaccg
     1141 cctgcggcgg cagcggacgc ggtgccatca cctgcccagc tggtgtggca aaaggttctc
     1201 cagtcaccac cgtcgacacc gccaagtcgg gcaatctggt gaactggaaa gccggggcgc
     1261 tgtaccacct gacggaaaac ggcaatgtct atattaacta tgccgtttcc cagcagcctc
     1321 cgggcggcaa caacttcgcc cttgcgcagt ctggcagtgg taacagtgcc aaccgcaccg
     1381 attttaaacc gcaaaaagcc aacaccagcg agattggcac caaatggcag gttctggata
     1441 aacgcctgtt gctcaccgcc gcgctgttcc gtactgatat cgaaaatgaa gttgagcaaa
     1501 atgatgacgg gacttactcg caatacggta agaaacgcgt cgaaggctat gagatatccg
     1561 tggcagggaa tatcactccc gcgtggcagg tgattggcgg ctatacccag caaaaagcaa
     1621 ccatcaaaaa cggcaaagat gttgcccagg atggttcctc atcgctgccg tataccccgg
     1681 agcacgcctt caccttatgg agccaatatc aggcaaccga tgatatctct gttggcgcgg
     1741 gcgcacgcta tatcggcagt atacataaag gttcagacgg cgcggtggga acgccagcat
     1801 ttacagaagg ttactgggtc gccgatgcca aactggggta tcgggttaat cgcaatctcg
     1861 acttccagct aaacgtttac aacctgtttg ataccgatta cgtcgcctca atcaacaaga
     1921 gcggctaccg ttatcacccg ggcgagccaa gaaccttctt gctcacagcc aatatgcatt
     1981 tctgattcag atgtggggcg caggccccac tttttggaga acttgtatga tgtaccacat
     2041 tcccggcgtg ttatcgccac aggacgtcgc ccattttcgc gaacaactgg aacaagccga
     2101 atgggtggat ggacgcgtca ccaccggagc acaaggtgca caagttaaga acaatcagca
     2161 agtagacacc cgcagcgcgt tatacgccgc actgcaaaat gaggtgctga acgcggttaa
     2221 ccaacatgca ttgttctttg ccgctgcctt gccgcgtacc ctttccacgc cgctgtttaa
     2281 tcgctatcag aacaatgaaa cctatggttt ccatgtggat ggcgcagtac gcagccatcc
     2341 acaaaacggc tggatgcgca ctgacctttc tgccacgctg tttttaagcg atccacaaag
     2401 ctacgacggc ggcgaactgg tcgttaatga cacctttgga caacatcggg taaaactccc
     2461 ggcaggtgat ctggtgttgt atccctccag cagcctgcat tgcgtgacac ccgtaacccg
     2521 cggcgtacga gtggcatcat ttatgtggat ccagtcgatg atccgcgatg ataaaaagcg
     2581 cgccatgctg tttgaactgg acaaaaatat tcagtcgctg aaaagccgct atggtgaaaa
     2641 tgaagagatc ctgtcgctgc tcaatcttta tcataatctg ctgcgggaat ggtcggagat
     2701 ctgacgcttg aactgcccag gttcgtccct atattagcta cacttaactg tacaagcatt
     2761 gatatgggga ggacgatatg gcatccgctt gggctaacga tgacgccgtc aacgaacaga
     2821 tcaacagtac aattgaagat gcgattgccc gcgctcgggg tgaaattccg cgtggcgaaa
     2881 gcctgtatga atgtgaagag tgcggtgccc ccatcccgca agcccgtcgg gaagccattc
     2941 ctggcgtgcg cttatgtatt cattgtcagc aggagaaaga tttacaaaaa ccagcgtata
     3001 caggatataa tcgcagaggt tcgaaagaca gacagttacg ttaactacaa ctggcgagat
     3061 gcatagcgag tataatttgt attttgcgtg gcattccgtg cctttaacgc tgctgtgact
     3121 gccacgcggc ataaaacaag tccataagta caacaaataa atggtttatc agtatgttag
     3181 atatcaatca atttatttga acaaggcggt caattctctt cgattttatc tctcgtaaaa
     3241 aaacgtgata ctcatcacat cgacgaaaca acgtcactta tacaaaaatc acctgcgaga
     3301 gattaattat gaaaactatc aatactgttg ttgctgctat ggctctttca actctgtcat
     3361 ttggcgtgtt cgcggcggaa ccggtaacgg catcccaggc acagaacatg aataaaatcg
     3421 gcgtggtttc tgccgatggc gcatccaccc tcgatgccct ggaagcga
//
LOCUS       DADLTY010000285         3456 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.289, whole genome shotgun sequence.
ACCESSION   DADLTY010000285 DADLTY010000000
VERSION     DADLTY010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.289"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(226..429)
                     /locus_tag="JN334_004157"
     CDS             complement(226..429)
                     /locus_tag="JN334_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440086.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="JN334_004158"
     CDS             complement(431..586)
                     /locus_tag="JN334_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 domain-containing protein"
                     /protein_id="HAY7440087.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="JN334_004159"
     CDS             complement(789..1196)
                     /locus_tag="JN334_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7440088.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="JN334_004160"
     CDS             1273..1500
                     /locus_tag="JN334_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7440089.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="JN334_004161"
     CDS             1484..1906
                     /locus_tag="JN334_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440090.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2761
                     /locus_tag="JN334_004162"
                     /pseudo
     CDS             1984..2761
                     /locus_tag="JN334_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2768..3456
                     /locus_tag="JN334_004163"
                     /pseudo
     CDS             2768..3456
                     /locus_tag="JN334_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          885 a          764 c          940 g          867 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg
     2461 tctaaaaaat tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg
     2521 catgagtggg tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat
     2581 acgggaactg gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag
     2641 catcgccaat gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct
     2701 ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta
     2761 acggaccatg aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc
     2821 gcaacatgta accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga
     2881 aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat
     2941 tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt
     3001 gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat
     3061 gaccgggtgt acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc
     3121 ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc
     3181 tgatgttatg agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt
     3241 gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac
     3301 gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag
     3361 cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa acattgctcg gcgagcgggt
     3421 gatggatcgc atggtcatga acggcgggcg ctgggt
//
LOCUS       DADLTY010000286         3413 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.290, whole genome shotgun sequence.
ACCESSION   DADLTY010000286 DADLTY010000000
VERSION     DADLTY010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.290"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..237
                     /locus_tag="JN334_004164"
     CDS             <1..237
                     /locus_tag="JN334_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HAY7440091.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            247..885
                     /locus_tag="JN334_004165"
     CDS             247..885
                     /locus_tag="JN334_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HAY7440092.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            888..2051
                     /locus_tag="JN334_004166"
     CDS             888..2051
                     /locus_tag="JN334_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY7440093.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2135..>3413
                     /locus_tag="JN334_004167"
     CDS             2135..>3413
                     /locus_tag="JN334_004167"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HAY7440094.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWGSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          758 a          803 c          988 g          864 t
ORIGIN      
        1 ttgttaggtt acgttatccc attatccagt gcggcaatca atgcggtgag tattccagac
       61 tatttcgcct ggggcgggat tgcactggtg attcagttac tcgtttttgc tggtgtcaga
      121 ctttatatgc ccgcattaag cgaaaaaatt attaatcaca ataccgcagc aggaatgttt
      181 atgggaaccg ccgcgctggc tggcggtatt tttaacgcag cttgtatgac atggtaatgg
      241 acgatcatgg ccagaaaacg caaatcaaga aataacagta aaattggtca cggagcgatc
      301 agtcgcattg gtagaccgaa taatcctttt gaaccgcgtc gcaatcgcta cgcacaaaaa
      361 tacttaacgt tggcgctaat gggcggtgcc gcttttttcg tattgaaagg ttgtagcgat
      421 agtagcgatg tcgataacga cggcgacgga acattttacg cgacagtgca ggattgtatt
      481 gatgacggta ataatgccga catttgcgcg cgtggctgga acaacgccaa agcggcattt
      541 tatgccgatg ttccaaagaa tatgactcag cagaactgtc agtctaagta cgaaaattgc
      601 tactacgaca atgttgaaca gagttggatc ccggtcgttt ctggattttt gttaagccgg
      661 gttattcgta aagatcgcga tgagccgttc gtctataaca gcggcggatc ctcatttgct
      721 tcccgccccg tctggcgcaa cacttccggt gattactcct ggcgcttcgg ttctggcaaa
      781 aaagagtctt actcttcagg cggctttacc accagaaaag cgtcgaccgt ttcgcgcggc
      841 ggctatggtc gttcttccag cgcccgtggg cattggggag gctaatcatg ctgagacaca
      901 acgttcctgt gcggcgggat ctggaccaga tcgccgctaa caacggtttc gactttcata
      961 tcatcgacaa tgaaatctat tgggacgaga gtcgggctta ccgttttact ctgcgccaga
     1021 ttgaagagca gatcgaaaaa ccgactgcgg aactgcatca gatgtgcctt gaggtggtgg
     1081 atcgcgcggt taaagacgaa gagatcctga cgcaactggc gatcccgccg ttgtactggg
     1141 atgtgatcgc cgaaagctgg cgcgcacgcg atccttcgct gtatggccgg atggattttg
     1201 cctggtgtgg caatgcgccg gtgaagctgc tggagtacaa cgccgatacg ccgacctcgt
     1261 tgtatgagtc ggcttatttc cagtggttgt ggctggagga tgcccggcgc agcggcatta
     1321 ttccgcgtga tgccgatcag tacaacgcca ttcaggaacg cctgatttcg cgctttagtg
     1381 agctttacag tcgggaactg ttttattttt gctgctgtca ggacaccgat gaagacagga
     1441 gtaccgtgct gtacttgcag gactgcgccc agcaggcagg gcaggagtcg cggtttatct
     1501 acattgaaga tctcggtttg ggcgtcggcg gcgtactgac cgatcttgat gataatgtca
     1561 tccagcgtgc atttaagctg tatccgctgg agtggatgat gcgtgacgat aacggtccgc
     1621 tgctgcgcaa gcgtcgcgag caatgggtgg agccgttatg gaaaagtatc ttgagtaata
     1681 aagggctaat gccgctgctt tggcgcttct tccctggtca tcctaacctt cttgcatcct
     1741 ggtttgaggg tgaaaaatcg cagattgccg ctggcgaaag ctatgtgcgt aaaccgatct
     1801 actcgcgcga aggcggaaac gtcaccattt tcgacggtca gaataacgtc gttgaccacg
     1861 ctgatggtga ttacgccgat gaaccgatga tctaccaggc gtttcaacct ctgccgcgat
     1921 ttggcgatag ctacacactc atcggtagct ggattgtcga tgatgaagcg tgcggaatgg
     1981 ggatccgtga agataacaca ctgatcacca aagacacctc acgtttcgtt ccgcattaca
     2041 ttgctggata agaatgtttt agcaatctct ttctgtcatg aatccatggc agtgaccata
     2101 ctaatggtga ctgccattga tggagggaga cacagtgcac tggcaaactc acaccatttt
     2161 taatcaacct ataccattaa ataacagcaa cttatacctg tctgatggcg cgctttgcga
     2221 agcggtaacg cgtgaaggtg ctggctgggg tagcgatttt ctagccagta ttggtcagca
     2281 gttaggaacg gctgaatccc ttgaactggg gcggctggcg aatgtaaacc cgcctgaact
     2341 gttgcgctac gatgcccagg ggcggcgtct ggacgatgtt cgtttccatc ccgcatggca
     2401 cctgctgatg caggcgttat gtaccaatcg ggtgcacaat cttgcctggg aagaagacgc
     2461 tcgctccggc gcatttgtgg cgcgcgcggc gcgttttatg ttacatgcgc aggttgaggc
     2521 tgggtcgtta tgtccagtaa cgatgacctt tgccgccacg ccattgttgt tacagatgtt
     2581 acccacgccg tttcaggact ggaccacgcc gctgctgagc gatcgctacg attctcactt
     2641 attgccaggt gggcaaaaac gcggtttgtt gattggcatg ggaatgacgg aaaagcaggg
     2701 cggttccgat gtcatgagca acaccacccg tgcagagcgt ttggaagatg gctcttatcg
     2761 gctggtgggg cataaatggt ttttctcggt gccgcaaagc gatgcgcatc tggtgctggc
     2821 gcagaccgcg ggcggtctgt cctgcttttt tgtgccgcgc tttttgcctg acgggcaacg
     2881 caacgcgatt cgcctcgagc ggctgaaaga taagctgggt aatcgctcta acgccagttg
     2941 cgaagtggag tttcaggatg ccattggctg gttattgggg caggaagggg aaggaattcg
     3001 tctgatcctg aaaatggggg ggatgacgcg ttttgattgc gccctgggta gccatgccat
     3061 gatgcgccgt gcattttcgc tggcgattta tcatgcacat caacgccatg tttttggtaa
     3121 tccattgatc caacagcccc ttatgcgtca tgtcttaagt cgcatggcac ttcagcttga
     3181 agggcaaacg gcgttgctgt ttcgtcttgc gcgagcgtgg gaccggcgtg ccgatgccaa
     3241 agaagccctg tgggcgcgtt tatttacgcc tgcggcaaaa tttgtgatct gcaaacgagg
     3301 tatgccgttt gtggccgaag cgatggaggt gctgggcggc attggttatt gcgaggagag
     3361 cgagctgccg cggctttacc gggagatgcc ggtaaacagt atttgggaag gtt
//
LOCUS       DADLTY010000287         3374 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.291, whole genome shotgun sequence.
ACCESSION   DADLTY010000287 DADLTY010000000
VERSION     DADLTY010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.291"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            223..882
                     /locus_tag="JN334_004168"
     CDS             223..882
                     /locus_tag="JN334_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HAY7440095.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1083..1460)
                     /locus_tag="JN334_004169"
     CDS             complement(1083..1460)
                     /locus_tag="JN334_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7440096.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1527..3293)
                     /locus_tag="JN334_004170"
                     /pseudo
     CDS             complement(1527..3293)
                     /locus_tag="JN334_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834018.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          704 a          894 c          988 g          788 t
ORIGIN      
        1 gacgcacttt gcgccgaata aatacctgtg acggaagatc acttcgcaga ataaataaat
       61 cctggtgtcc ctgttgatac cgggaagccc tgggccaact tttggcgaaa atgagacgtt
      121 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta
      181 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      241 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      301 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      361 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      421 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      481 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      541 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      601 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
      661 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
      721 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
      781 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
      841 gaattacaac agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat
      901 tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg ataataagcg gatgaatggc
      961 agaaattcga aagcaaattc gacccggtcg tcggttcagg gcagggtcgt taaatagccg
     1021 cttatgtcta ttgctggttt accggtttat tgactaccgg aagcagtgtg accgtgtgct
     1081 tctcaaatgc ctgaggccag tttgctcagg ctctccccgt ggaggtaata attgacgata
     1141 tgatcattta ttctgcctcc cagagcctga taaaaacggt tagcgccggg gttggatttt
     1201 tcagcgttcc agctaaggct aaggcattcc tgttcaagcg caagccgggc tataaagcgc
     1261 attatcgctt tacccgtgcc cttatttcga tcgcactgag aaacatacag ctctttaata
     1321 tgcagctgac cgctgtatcg gggcgaggga taaagaatat tgcaacatgc caggccagta
     1381 atgttattgc cgcagcgcgc tctgatcacc agggtaccgg aaagccggtt gaataacttt
     1441 ttacagagat aatccttcat caacgcttcc tgaatgatac cttcgccata ataggggtcg
     1501 tctcagaaaa cggaaaataa agcacgctaa gccggttgca gaggccgtag cggcctgaac
     1561 ttccccgcgc cgatcttggc gctgctgcgc cataggtaat caccggtcag gttgatgtgc
     1621 tcccagccga gtggcgacag gtactgcaat agcgagtcat cgacggcatg accattgccg
     1681 cgcaacgcat gcgccgcacg ctccaggtag accgtgttcc acagcacgat ggccgccgtc
     1741 accaggttga ggccgctggc ccggtagcgc tgctgctcga aactgcggtc acggatttca
     1801 ccaaggcggt tgaagaacac ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc
     1861 ccggcatgca cgcggcggcg tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg
     1921 cgctcgatgc ggcccaactc gcgcagcgcg acggccaagc cgttctggcg cgggtagctg
     1981 ccgagtttcc tgagcatcag cgaggccgtc accgtgccct gcttgatcga ggtggccagc
     2041 cgcaggattt cgtcccaatg ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatg
     2101 ggtttcagcg cgtcataggc ggcgtcgccc ttcgggatgt agagcttggt gtcgcccagg
     2161 tcgcggatgc gcggcgcgaa gcggaagccc aggaggtgca tcagggcgaa gacgtgatcg
     2221 gtgaagcccg ccgtgtcggt gtaatgctcc tcgatccgca agtcggactc gtggtacagc
     2281 aggccgtcga gcacgtaggt cgaatcgcgc acgccgacgt tcacgacctt ggtgtgaaat
     2341 ggcgcgtact ggtcagaaat gtgggtgtag aacgtccgcc ctgggctgct cccgtatttc
     2401 gggttgatgt ggccggtgct ctcggccttg ctgccggtgc ggaagttctg gccgtccgac
     2461 gatgaggtgg tgccgtcgcc ccagtgctcg gcgaagggat ggcggaactg tgcgttgacc
     2521 agatcggcca gcgccgcccc gtaggtttcg tcgcggatgt gccaggcttg cagccaagcc
     2581 agcttggcgt aggtcgtgcc ggggcaagac tccgccatct tggtcaggcc caggttgatc
     2641 gcgtcggcga ggatcgtggt cagcaacagg ttcttgtctt tggccgggtc gcccgatttc
     2701 agatgcgcga aatgccgagt gaagcccgtc cattcgtcca cctccagcag cagttcggtg
     2761 atcttgacgt gcggcaggat cattgccgtc tggtcgatca gcgcttgggc ggtgtcgggt
     2821 accgccgcgt cgagcggcgt gatcttcaag cctgactcgg tgatgatggc gtccggcagc
     2881 tcgttggccg tcgccatacg gttgacggtg gcaagctgtg tttccagcag cgtcagccgg
     2941 tcgttcaggt accggttgca gtcggtggcc acggccagcg gcaattcgct ggcctgcttg
     3001 aggctggcga atttcgcggg cggcaccagg tagtcctcga agtccttgaa ctggcgcgac
     3061 ccctgcaccc agatgtcgcc ggaacgcaac gcgttcttca tctccgacag cgcgcacagt
     3121 tcgtagtagc gccggtcgat gccggtgtcg gtcatgacca gcttctgcca gcgcggcttg
     3181 atgaactcgg tcggcgcgtc ggcgggcacc ttgcgggcgt tgtcgctgtt catgccgcgc
     3241 agcacctcga tggcgtcgag cacgtccttc gcggcgggag cggcccgcag ctggcactgt
     3301 tgcaaagtta gcgatgaggc agccttttgt cttattcaaa ggccttacat ttcaaaaact
     3361 ctgcttacca ggcg
//
LOCUS       DADLTY010000288         3348 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.292, whole genome shotgun sequence.
ACCESSION   DADLTY010000288 DADLTY010000000
VERSION     DADLTY010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.292"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            19..897
                     /locus_tag="JN334_004171"
     CDS             19..897
                     /locus_tag="JN334_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7440097.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="JN334_004172"
     CDS             894..2285
                     /locus_tag="JN334_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAY7440098.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="JN334_004173"
     CDS             2282..2539
                     /locus_tag="JN334_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440099.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="JN334_004174"
     CDS             2634..2834
                     /locus_tag="JN334_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HAY7440100.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="JN334_004175"
     CDS             2827..3207
                     /locus_tag="JN334_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7440101.1"
                     /translation="MRDIQQVLERWGARVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          886 a          726 c          969 g          767 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgagggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaac
//
LOCUS       DADLTY010000289         3324 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.293, whole genome shotgun sequence.
ACCESSION   DADLTY010000289 DADLTY010000000
VERSION     DADLTY010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.293"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            454..1281
                     /locus_tag="JN334_004176"
     CDS             454..1281
                     /locus_tag="JN334_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAY7440102.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKPGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1325..2074
                     /locus_tag="JN334_004177"
     CDS             1325..2074
                     /locus_tag="JN334_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY7440103.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2071..2640
                     /locus_tag="JN334_004178"
     CDS             2071..2640
                     /locus_tag="JN334_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440104.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2661..2759
                     /locus_tag="JN334_004179"
                     /pseudo
     CDS             2661..2759
                     /locus_tag="JN334_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            2764..3006
                     /locus_tag="JN334_004180"
     CDS             2764..3006
                     /locus_tag="JN334_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY7440105.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3165..>3324
                     /locus_tag="JN334_004181"
     CDS             3165..>3324
                     /locus_tag="JN334_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY7440106.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPK"
BASE COUNT          928 a          797 c          854 g          745 t
ORIGIN      
        1 acgccatcct gacaagcgtt ttatcttcaa cgggaatgag atatggaaac gtggagaatc
       61 aaccacagag tatacccgcg cacaccagcg aacaatcaga cgactgcgca gactcatcgc
      121 caggaaacag tatacagaaa aatggttcag aaaatatgac acatggagcg ccggatatta
      181 cgaactgatg gcaacaaaac cattctgacg taaacgaaat taaccatgac gcaattaaat
      241 aaggcaagcc gaatacatca ggaggaccat gaacatttat ttcagaatag ttatatcact
      301 ggcaattatc gcatgtattt acggattaat cgttccggcc ctcatatcaa tgaaggatac
      361 ggtagcagtg atttctggct ttgctctggc gtgtctgacc ccgccctgca tttatgccat
      421 ttataagggt ctttcttttt ctaaggataa aagatgaaaa aagttattcc tgctttagcc
      481 cttgttctgc tgaccaccgg acttgtcggt tgcgatcgcg ttgagcctgg taatgttggc
      541 atcaaggtaa acaaaccggg cgacgataaa ggcgtcggag aagtggtcgg tgttgggcgc
      601 tactggacgg gatggaacac tgaggtttac atcttcccga ccttcaaaca aatgaagacc
      661 tacgatgagc cattcagctt ccagatgagt gacggtacaa ccatcggcta tcacatcggc
      721 gtggcctaca aagttgatcc atccaaagtt accacagtct ttcagaccta ccgcaaaggc
      781 gtggacgaca ttaccgacac cgacctgcgc cagaagatag ccgacgcact caaccgactg
      841 gccagcaaaa tgaccaccga caaatttatc gacggcggca agtctgagct gctggatgca
      901 gctcttaaag acattcagga agaaatgacg cccatcggta ttcaggtaat gagcctctca
      961 tatgtgggta agccggagta cccgcctact gttatcgaca gcattaatgc caaagtcacg
     1021 gcgaaccaga aaaccctgca atgcgagcag gaagtaaaac agcgcgaagc ggaagccaac
     1081 atgttgcgcg cggaagctgc cggacaggca gatgcgattc gcacaaaagc ccaggccgaa
     1141 gccgatgcta ttcgtttacg cggtgaagct ctgcgccaga acccaaacgt tatggagctg
     1201 gaagccatca acaagtggaa cggtacactg ccgcaataca tgaccagcgg tgccaacaca
     1261 ccatttattc aggttaagta atacatatgc ccggtattac acgccgggct gtctggagat
     1321 aaaaatgaat actgtaacca tcaacaacaa acagtttccg gtaatcgaat atcgcggtca
     1381 gcgtgttgtg acgctggcga tgattgatga agtccaccag cgaccggaag gaaccgcgcg
     1441 tgcagcattc aaccgaaacc gtgagcattt catcagcggt gtggattatg ccgaattagg
     1501 tgcggacgta atacgtacgg acctcccgga aggaacattc tctaaatttg caccatcggg
     1561 gattgtactt tttgaatcag gttacctgat gttgacgaag ccattcaatg acgcccttgc
     1621 ctggcaggtt cagcgcgaac tggttaacag ctacttccgc actcgcacac cgctaacgga
     1681 aatcgagatg atcgctgcga tagccgccga cgctgttcgt caacagaagc gcctgaatca
     1741 tgtggaagaa cagctcgaaa cagtcacaga agcggtggaa accatcaaac gcggaaatat
     1801 gcgcgccgga tatgtcggtt accgccaggt ggtagccaaa agtggaatga gtgacaccaa
     1861 gtgtcggaat ctggtgaacg catatcaaat tcctacagac acccacgaat tcatgactcc
     1921 ggacggtctg ttgtctcgca gggctatcgt cgagcttgag ccatttatgg cggcgttcca
     1981 ccaaatgatg tcagaggctg acccacgcgg cacacgctgg tatcacccga agatggggct
     2041 tttccaggtg atcggatggg aggataaagc atgatcatcc agtcaaaact tattcgcgcc
     2101 gctctggtgt gcgctgctaa aaacgacgtt cgttattacc tgaacggtct tcacatcacg
     2161 ccgaaacata ttgaggcaac caatggttcc gtggcactgc gcatggctca cggcatccgg
     2221 acgaagaaaa acatcattgt ccagttcgaa gggggcgtcc cggccaaagc cgaaacgaca
     2281 gagctgattt ttagtaaaga gccgatcgct gttcatcgcg accagtttca gcgacgactg
     2341 tccattaccg gcattaaact ggtggacggt tgttttccgg atttggatcg catcattccg
     2401 aaaaagtttg accgctgtac tcacccggtg ctccaggcgg gttacctgag ttatccagag
     2461 aagatgtttg gtcgtgagcg taaatttatt cccgtccagt tacgtccctc cggtgacggg
     2521 caagcggtca gaattcagtt tgattccatc atcaactcaa tgtatggcaa tcctgaattt
     2581 gttgtgatgc cttgtcgtga tcatggcgat ttcaacgtgg ctcaggagca tccggaatga
     2641 aaatcgaata ccaggacgcc gcaggaggtg aatcaatgag ctggcctgat gcaatcgtaa
     2701 ctctggggat ggtattcgca gtagcgtttg ttgtgtactc gatttgtcga tggggataac
     2761 cacatgttcg ctttgattca acgcgggcag atatacacag acagagccgg atacccagta
     2821 gaaattactc gcagtactga gcactcagtg ttctttcgga ggatggacgg acgtaccggg
     2881 cgagtacgca tcggtgaatt cagtagcctg ttcgagcata ttgaccacct ggaatatcac
     2941 aagattctgg ctgaaacaga acaagaaaag cacctgaaga aattacgcgc aatgcaaagg
     3001 aagtaaaaaa tgaataaagc gtttgagcta tgggtccacc agcgttacgg caaccactac
     3061 gacctgacgc gagatgttga cggtttctac tgtcgtgaaa ttgtgaaacg aatgtttgag
     3121 gtctggtgcc actgccgtgg gctgagtgtt gtgtgaggta atgcatgggc aatgtgattc
     3181 aactggctcc caatgaatgg gtttgtgaaa gcgttctaat cgcaattacc gggctcaaac
     3241 caggcacaat tcttcggacc cggaaagaat gctggatggt tggaagagag tatattcacg
     3301 tatcaccaga cggtaatcca aagc
//
LOCUS       DADLTY010000290         3285 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.294, whole genome shotgun sequence.
ACCESSION   DADLTY010000290 DADLTY010000000
VERSION     DADLTY010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.294"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(45..992)
                     /gene="icsP"
                     /locus_tag="JN334_004182"
     CDS             complement(45..992)
                     /gene="icsP"
                     /locus_tag="JN334_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HAY7440107.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(2373..2525)
                     /locus_tag="JN334_004183"
                     /pseudo
     CDS             complement(2373..2525)
                     /locus_tag="JN334_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2581..3274
                     /gene="ospZ"
                     /locus_tag="JN334_004184"
                     /pseudo
     CDS             2581..3274
                     /gene="ospZ"
                     /locus_tag="JN334_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
BASE COUNT         1037 a          605 c          577 g         1066 t
ORIGIN      
        1 ggcaattttt agccatgaaa acgtaatcaa ctcggtgaac caattcaaaa aatatacttt
       61 atacctgcgg aagtgagaaa accaatatat tcaatacttg cactattttt aatggtgcca
      121 gatatattgg tactttgctc ataaagagat gtatcacctt ttttattaga aatgtaattt
      181 ctggagcctt ctatataaaa ttttgcactg ggggtgacat agtatccaat attcgcagca
      241 acagagcagt aattttggtt ttttatttca tctttaaaaa ttgtctcagt ctgatagtgt
      301 ttatctgtat cagatgaaag aacccaacca ctatatttca gttctgctcc aaactcaaaa
      361 ttctcatggc ggtaattagc agttaatcca atataaggta ttttgaagag ctgtttgtaa
      421 cctattgttc tttcaccact aggatgtgcc ccttttttat tcctgctccc cccgttttca
      481 ctataaatat aactccctcc cattgcatta aaactgtaac gactctcttg gtaacctgct
      541 attagtccaa gtcgataatc caaattattt aataaccaac ctttaagatt cagctcgaat
      601 tcgttagcat cacggagatg cgtattggga tgccagctct ggtcggtcca tacctgaggg
      661 gtattggaat tgttccagtc tttatcaacc atgcttgctt tctggttacc taaagtagtc
      721 caaccggaaa ctccgaaaga cacttttgga attagcttcc aatcgatgcc acctctaaca
      781 atagtcgcat tactgtattt ccagtccagt tgactaattt ttctcccccc ttccttggga
      841 tgataaacgc gttcttttgt tttgccactc agagatccta gactaatatc cgtactgatg
      901 ttgtccggta tgaaaagtgg atagttagtg gtagcatgtg tagtaaagat cgcaggtaca
      961 caaagtgcaa gtacaaagaa ttttaatttc atcgagaact ctactttttt ggttgaaatg
     1021 tccatgatct tcttacttta taagataaac gtcaatgcca agcattattt taattaaata
     1081 tttgtttatg ttaccaagaa catatctttt attataatat ttattgaact tttgtaaaaa
     1141 taaaaaaata cctcattgtg cgaataaagt aacgggggcc atagatttct ctacctacct
     1201 tttggtgttc atacctgagg ctgatagttt caggattccg cgacaattct actcaccttc
     1261 ttgttctctc gccaatatga aatcaatcag aaaagcgttc cataagaaca acgataagac
     1321 aataaattgt catcacggct gcaatagcca acgtgccttt cagtaccaac atgacaacct
     1381 cctgtatttg acgtaacgtg tgctgataaa tatgtatttg tatctcaact gactcaatac
     1441 aacacttaag tatagttttt cagattttgt ttttcccgtg aaatattatg gaaatggcag
     1501 aagcaataca ttgaattccg attcgagtga atacggctag cgggcacctc actttagcac
     1561 tgaagccatc aacttttatt tgacttattt caatggaaag ctttctcact atcagtacag
     1621 gtaattccca tccttttaaa gtcgagagtc agactgcgac taatacagtt aaagtgacca
     1681 aactcagtta tcctctacac attttatctc atgtttactt ggacaattca ttggtgcttt
     1741 tataaaagaa taaatattct actaatttaa tgcatcacct aaaatagtat tttttataat
     1801 agaactgtat ctatagtata ttattaaagc ttaattattt attatgcttt aatgtggttg
     1861 tccgattaag gactaatcct tgaaaacgcc gtatagggcg agagttcact ctattgcgat
     1921 aacgtaaccg tacaatattg tgcgtttccc aaattgacca ctactgaact attctgggat
     1981 ttaaatgcaa taaaatggtt ggttgaaggt cgtggtgatg atgttagtcc ttttgagaat
     2041 attccagtgc cagatcttac ttataataaa gaggcttggc agtttggata agctattgtc
     2101 cttattccac taatacaaac tttttgtaaa gtatattaaa tatacttcat ttcatactga
     2161 aatccccgga agtattcaat caaacaatta caccataaaa ggaagaggga gccccaatta
     2221 agttatcaag catgtttatg atacctgcag ggtataaatt ttctggctgc aagcagacac
     2281 atataaaacg tctttattga tcctacccac ataatatgga cgcagtcgta agtgaggctc
     2341 tgtgagccta cccacgtaat atggacacag gcctaagcga ggttctggtt ttcaaattgt
     2401 tccggactga ggccgccaca ccaacggtgc cgtcgccagc gattgtaatt gcactcgata
     2461 taattaaaca ctgttgcccg cactatttcc cgatagataa agtgttctcc gtggagacat
     2521 tccacttagg aacaattata ttgttcatgg taatgtttat atttgagtat agagattaaa
     2581 atgattagtc ccatcaagaa tattaagaac gtatttccaa taaatactgc aaatactgaa
     2641 tatatagtga gaaatattta tcctagagtt gaacatggtt actttaatga atcaccaaac
     2701 atatatgaca agaagtatat ctctggtata accagaagta tggcacaatt aaaaatagag
     2761 gagtttatta atgagaaatc cagacgactt aattatatga agacaatgta ttctccatgt
     2821 ccggaagatt ttcagcctat ttccagagcc gaagccagta caccggaagg aagctggttg
     2881 acagttatat ctggaaaacg tcctatgggg caattttctg tggatagttt gtaccatcct
     2941 gacttacatg cattatgtga gctcccagag attagttgta agatcttctc taaagaaaat
     3001 agtgattttt tgtatataat tgttgtattc agaaatgatt cttcccaagg agaactacgg
     3061 gcaaatcggt ttatagaact atatgatata aaaagagaga ttatgcaagt gttaagagat
     3121 gagtcgccag agattaaagt ctattaaatc tgagattatt attgctcgtg aaatgggaga
     3181 actttttagt tatgcatcag aggaaataga tagctacata aaacaaatga atgacaggct
     3241 ttctcaaatc aaggctcgaa tgcctgttac ttaatgtata ttaat
//
LOCUS       DADLTY010000291         3258 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.295, whole genome shotgun sequence.
ACCESSION   DADLTY010000291 DADLTY010000000
VERSION     DADLTY010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.295"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            1..442
                     /locus_tag="JN334_004185"
                     /pseudo
     CDS             1..442
                     /locus_tag="JN334_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847259.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1026..1742
                     /locus_tag="JN334_004186"
     CDS             1026..1742
                     /locus_tag="JN334_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440108.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2294..2698
                     /locus_tag="JN334_004187"
     CDS             2294..2698
                     /locus_tag="JN334_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440109.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2761..3258
                     /locus_tag="JN334_004188"
                     /pseudo
     CDS             2761..3258
                     /locus_tag="JN334_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          909 a          675 c          749 g          925 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagccccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccc ggtttaatct
      301 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      361 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      421 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      481 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      541 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
      601 ttaatgtttt gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa
      661 caagtaaaaa caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct
      721 cttgcaacac atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat
      781 gagccctaac tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta
      841 ccaactagcg ttactaaatg ttgaccaagt tttgttcact tgtctggttt tgatagtagc
      901 attctgcaga gtattttgat atgataaata tgcattaaca atgatattat tgttaatttc
      961 ctagcatgaa aattaaaaga tcaactttta tctcgaatat tttttacatt atcagttagt
     1021 ttcttatgaa tgacaattca ttgcttagaa attcttcatt attcatcgcc tatatgggat
     1081 gtgtagggtg ggtcagtgct tattcttatg gctggggaac ctctttttac tatggatttc
     1141 catggtgggt tgttggtgca ggtttggatg atgttgccag aagtttactc tatgctatca
     1201 tagtaatggg tatacttttt accggttggg ggattggtat tttatttttt cttcttataa
     1261 agaaaagaag taaaatacaa gacttgagtt tttttagact attttttgct attaccctac
     1321 tgtttttccc tgtaatattc gaacttttaa ttctcaaaca atatttcata cttccattat
     1381 cactttcatt cattattagt tcattggtta tttcaataat aattcgtata tatggacgga
     1441 tcttttctgt atcatgtttt tcagatatcc cttttgttag agaacaccgc attaagttaa
     1501 ttatggctgg ttttttggtt tatttttggt ttttttcctt tcttgttggc tggtataaac
     1561 ctcaattaaa aaaagaatat cagatgcttt gttataataa tagttggtat tatgttcttg
     1621 ctcgttatga ttcacggctc gttttatcta gttcatttaa agatgacagt aacagattct
     1681 taatatttaa tacagaacag tcgggatttt atgaaatcaa tgatgtttat gtcagaaagt
     1741 aataatcctc tcccacaaag tgaattaaat gaatatataa tgcgttcgaa gtatatgtat
     1801 tgagctgtca tactgagaaa caaaccttag ggcagcacaa ttaaagccag aaacaactat
     1861 gcaagaaagt tactaaataa gtgctcggaa cagtaaaaca ctcagggggg actcagtccg
     1921 gattatgtaa tcagataaat tgccaaacat cacaaatcac cagccggact gagtgatgcc
     1981 gagcattgca ccaatacccc gtgatgaacg atgcctgatg cagaaagctc cctataaaac
     2041 aaagaacaaa aactacgacc ggcgattcac cgtcatactg atgctgcatc gtggtgacac
     2101 tgtctgtcac tttcatgttg actgaacaac tctgctgcga agaaggtgta ccgccggcgg
     2161 gatttgattg tccggcgtct agcttccact tccctgatgg aactgaaagt tgttatggcc
     2221 ggtcagcttg ccctgtccgc aggacatgag gtgctgtgac ggacagcacc agtaacggaa
     2281 agtgacaatg aaaatgaata ctgcgctgat agttgcacta atgtgtatgt ggtatgcggt
     2341 acccgctgct gcaaaagaga ctttactggc tatgccacgt aattcaacag aacattgcta
     2401 tgcggagatt aatgtgcacg gaccatatgg ggtgtatttc cgagtggtcc cgcatccccc
     2461 cgggggaaag agctgggtcg aatgtaacag tgactactat tactctgata aaccaccggg
     2521 agtacagata ctcggaaccc gtgcagggtg tcgggtatac ggaatctgcg ggaccacctc
     2581 gacactgcat gtggctggaa gaggggttgt gtgtattaaa aatatatgca gtccacgtgg
     2641 gatgataatt cacaggatac ggaaacgtcc tgtagtggct gtaagcgatg agatgtgatc
     2701 ttacccagca atagtagaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac
     2761 atgacaaaaa cagtatcaac cagcaaaaaa acacgtaaac agcattcgcc tgaattttgc
     2821 agtgaagccc tgaagcttgc tgaacgcatc ggtgttgctg ccgcagcccg tgaactcagc
     2881 ctgtatgaat ctcagctcta tgcgtggcgc agcaaactgc agcaacagat gacctcgtct
     2941 gagcgtgaaa gtgaactgcc cgcctgaagc ggcaactggc ggagcaggca gaggaactgg
     3001 ctatcctcca aaaggccgcg acatactttg cgcagcggct ggtatacgtg gtgtcagcgt
     3061 cggacaggaa taagccctcg tcagcagttc cgtcagcact gcgacagcgt tgtcctcgcg
     3121 gcggccttta cccggtcaaa acagcgttac ggtgcaccac gcctgacgga tgaactgcgt
     3181 gctcagggtt accactttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcaggggctg
     3241 agggcaaagg cctcccgg
//
LOCUS       DADLTY010000292         3254 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.296, whole genome shotgun sequence.
ACCESSION   DADLTY010000292 DADLTY010000000
VERSION     DADLTY010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.296"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..203)
                     /gene="mokC"
                     /locus_tag="JN334_004189"
     CDS             complement(<1..203)
                     /gene="mokC"
                     /locus_tag="JN334_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HAY7440110.1"
                     /translation="MLNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYES"
     gene            732..1898
                     /gene="nhaA"
                     /locus_tag="JN334_004190"
     CDS             732..1898
                     /gene="nhaA"
                     /locus_tag="JN334_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HAY7440111.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1964..2863
                     /gene="nhaR"
                     /locus_tag="JN334_004191"
     CDS             1964..2863
                     /gene="nhaR"
                     /locus_tag="JN334_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HAY7440112.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2901..>3254)
                     /locus_tag="JN334_004192"
     CDS             complement(2901..>3254)
                     /locus_tag="JN334_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440113.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT          762 a          734 c          797 g          961 t
ORIGIN      
        1 gattcgtaag ccgtgaaaac agcaacctcc gtctggccag ttcggatgtg aacctcacag
       61 aggtcttttc tcgttaccag cgccgccact acggcggtga tacagatgac gatcagggcg
      121 acaatcatcg ccttatgctg cttcattgct ctcttctcct tgaccttacg gtcagtaaga
      181 ggcactctac atgtgttcag catataggag gcctcgggtt gatggtaaaa tatcactcgg
      241 ggcttttctc tatctgccgt tcagctaatg cctgagacag acagcctcaa gcacccgccg
      301 ctattatatc gctctcttta acccattttg ttttatcgat tctaatcctg aagacgcctc
      361 gcatttttgt ggcgtaattt tttaatgatt taattattta actttaattt atctcttcat
      421 cgcaattatt gacgacaagc tggattattt ttgaaatatt ggcctaacaa gcatcgccga
      481 ctgacaacaa attaattatt acttttccta attaatccct caggaatcct caccttaagc
      541 tatgattatc tgggcttagg gtcactcgtg agcgcttaca gccgtcaaaa acgcatctca
      601 ccgctgatgg cgcaaattct tcaatggctc gtaaaaaacg aattattcct acactataat
      661 ctgattttaa cgatgattcg tgcggggtaa aatagtaaaa acgatctatt cacctgaaag
      721 agaaataaaa agtgaaacat ctgcatcgat tctttagcag tgatgcctcg ggaggcatta
      781 ttcttatcat tgccgctatc ctggcgatga ttatggccaa cagcggcgca accagtggat
      841 ggtatcacga ctttctggag acgccggttc agctccgggt tggttcactc gaaatcaaca
      901 aaaacatgct gttatggata aatgatgcgc tgatggcggt atttttcctg ttagtcggtc
      961 tggaagttaa acgtgaactg atgcaaggat cgctagccag cttacgccag gccgcatttc
     1021 cagttatcgc cgctattggt gggatgattg tgccggcatt actctatctg gcttttaact
     1081 atgccgatcc gattacccgc gaagggtggg cgatcccggc ggctactgac attgcttttg
     1141 cacttggtgt actggcgctg ttgggaagtc gtgttccgtt agtgctgaag atctttttga
     1201 tggctctggc tattatcgac gatcttgggg ccatcattat catcgcattg ttctacacta
     1261 atgacttatc gatggcctcc cttggcgtcg cggctgtagc aattgcggta ctcgcggtat
     1321 tgaatctgtg tggtgtacgc cgcacgggcg tctatattct tgttggcgtg gtgttgtgga
     1381 ctgcggtgtt gaaatcgggg gttcacgcaa ctctggcggg ggtaattgtc ggcttcttta
     1441 ttcctttgaa agagaagcat gggcgttctc cagcgaagcg actggagcat gtgttgcacc
     1501 cgtgggtggc gtatctgatt ttgccgctgt ttgcatttgc taatgctggc gtttcactgc
     1561 aaggcgtcac gctggatggc ttgacctcca ttctgccatt ggggatcatc gctggcttgc
     1621 tgattggcaa accgctgggg attagtctgt tctgctggtt ggcgctgcgt ttgaaactgg
     1681 cgcatctgcc tgagggaacg acttatcagc aaattatggt ggtggggatc ctgtgcggta
     1741 tcggttttac tatgtctatc tttattgcca gcctggcctt tggtagcgta gatccagaac
     1801 tgattaactg ggcgaaactc ggtatcctgg tcggttctat ctcttcggcg gtaattggat
     1861 acagctggtt acgcgttcgt ttgcgtccat cagtttgaca ggacggttta ccggggagcc
     1921 ataaacggct cccttttcat tgttatcagg gagagaaatg agcatgtctc atatcaatta
     1981 caaccacttg tattacttct ggcatgtcta taaagaaggt tccgtggttg gcgcagcgga
     2041 ggcgctttat ttaactccac aaaccattac cggacagatt cgagcgctgg aagagcgcct
     2101 gcaaggcaaa ttatttaaac gcaagggacg tggtctcgaa cccagcgagc tgggagaact
     2161 ggtctatcgc tatgccgata aaatgttcac cttaagccag gaaatgctgg atattgtgaa
     2221 ctatcgcaaa gaatccaatt tattgtttga cgttggcgtg gctgatgcac tttccaaacg
     2281 cctggtcagt agcgtactta acgccgcagt ggtagaaggc gagcccattc atcttcgctg
     2341 cttcgaatcc acccacgaaa tgctgctgga gcaattaagt cagcataaac tggatatgat
     2401 catttctgac tgtccgatag actctacgca gcaggaaggc ctgttctccg tgagaattgg
     2461 cgaatgtggc gtgagtttct ggtgtacaaa tccaccacca gaaaaaccgt tcccggcttg
     2521 tctggaagaa cggcgacttt tgattcctgg gcgacgttca atgttagggc gcaaattgct
     2581 taactggttt aactcccagg gattaaacgt agaaatcctc ggcgagtttg atgatgccgc
     2641 tttgatgaaa gcttttggtg cgatgcacaa tgcaatcttc gttgccccaa cgctttatgc
     2701 atatgacttt tatgccgata aaactgtcgt agaaattggt cgcgtcgaga atgtgatgga
     2761 agagtaccat gctatttttg ctgagcggat gattcagcac ccggcggtac agcgaatctg
     2821 caatacggat tattctgcgc tttttagtcc agcggtgcgt taatcggcag ctcccccaaa
     2881 gttaaggtgg gggagataga ttagttgtac attaccacga ttttgactcg gctcattatt
     2941 tgcccgcttg agacattgtt tccatatgta cgcgggcgaa taaatagagg aatctgatta
     3001 cttccttcat ggggatgctg aaaagagtag taattgcttt taccatactg gatttcattc
     3061 gtaccatcat tggagagttg aaagcctatt tgacccgcac cgtcctcact gttttctaac
     3121 tcatttttta atatgctggt tcctgactcc tgtatcgtca ctggttcaaa gtaatattca
     3181 acgtcactgg gttcaaaaac taattcacct tgctcgccct ggatgcagtc aaatcttata
     3241 tgatgaaact gata
//
LOCUS       DADLTY010000293         3236 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.297, whole genome shotgun sequence.
ACCESSION   DADLTY010000293 DADLTY010000000
VERSION     DADLTY010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.297"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(64..528)
                     /gene="soxR"
                     /locus_tag="JN334_004193"
     CDS             complement(64..528)
                     /gene="soxR"
                     /locus_tag="JN334_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HAY7440114.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            614..937
                     /gene="soxS"
                     /locus_tag="JN334_004194"
     CDS             614..937
                     /gene="soxS"
                     /locus_tag="JN334_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HAY7440115.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(940..2517)
                     /gene="pdeC"
                     /locus_tag="JN334_004195"
                     /pseudo
     CDS             complement(940..2517)
                     /gene="pdeC"
                     /locus_tag="JN334_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            2946..3227
                     /locus_tag="JN334_004196"
     CDS             2946..3227
                     /locus_tag="JN334_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440116.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          866 a          795 c          708 g          867 t
ORIGIN      
        1 gcaaatattt accctcaccc cggccccata gttaacgatg agtcagcttg tccttgtggc
       61 gctttagttt tgttcatctt ccagcaagcg tgcgccggta ccttcttctc ctaagcggtc
      121 gcccgggtta cgcaacgggc aatcactgcg cgaaaggcag ccacaaccaa tacatccgtc
      181 cagttcgtca cgcagcgcca ctaaggtatg aatgcgccga tccaactctt ctcgccattg
      241 ggacgaaagc tgtttccact ctttcgcact taacgtatgc ccttctggca acacgccaaa
      301 cgcttcacca atggtcgcca gcggaatgcc aatacgctga gcgattttga taattgcgac
      361 atatcgcaac acatcacgtt tatatcgccg ctgattgccg ctgttacgga tactagtaat
      421 caaccctttg ctttcataga aatgcagcgc cgataccgcc acaccgctgc gtttcgccac
      481 ttcgccaggg gttagcagcg ctttaatgcg gggtaatttc ttttccataa atcgctttac
      541 ctcaagttaa cttgaggaat tatactcccc aacagatgaa ttaacgaact gaacactgaa
      601 aagaggcaga tttatgtccc atcagaaaat tattcaggat cttatcgcat ggattgacgt
      661 gcatattgac cagccgctta acattgatgt cgtcgcaaaa aaatcaggct attcaaagtg
      721 gtacttgcaa cggatgttcc gcacggtgac gcatcagacg cttggcgatt acattcgcca
      781 acgccgcctg ttactggccg ccgttgagtt gcgcaccacc gagcgtccga tttttgatat
      841 cgcaatggac ctgggttatg tctcgcagca gaccttctcc cgcgttttcc gtcggcagtt
      901 tgatcgcact cccagcgatt atcgccaccg cctgtagttt tattgcccgc gcgttaactc
      961 ccgcgcgggt aattgctcca tccattgcat aaacacctgc ggcggcatcg ccttcgcaaa
     1021 aaaccatccc tggcaatagc gcacgccgcg tttgcgcagc cagttaacct gctcctcagt
     1081 ttcgacgcct tcagcgatcg tttttaaccc caggctgtgc gccagctcga tgatgtgttc
     1141 cgcaatcaaa tgactggttt tgtgggtggt cagcgtttca acaaacgatt tatcgatttt
     1201 caaaatatcg acattcaatg atttaaggtt atgcaagtta gagtagccaa taccaaaatc
     1261 atcaattgcc acttcgtaac cagcctggcg gaaagccaga attaatcggc gtcatttttt
     1321 cgacatcaag aaatgcatgc tcagtcactt caaatttaat ttgctgcgga cgcaccgcgt
     1381 attgctctgt tttctgattg attcgcgcta tcaaccgtga cgtatgaaaa tcggaggccg
     1441 acaggttaat agaaacatag cgatcggcat gtgttgccag gtacgcgccc agatcgcgga
     1501 agacattatc aataacataa tcagttatct gttctatcat cccctccttt tctgccagcg
     1561 gaataaactc tgccggattc attatttgcc cctgctcacc aggccaacgt aacaacgctt
     1621 cagcgccgat acatttttct gttttgatat cgattattgg ctggtaataa agacaaagtt
     1681 gatgtttttc gagggcgcgt tgcaatttac gtttgggaga taaatagttt tgtcgaatac
     1741 gtagccagag taatagcaga acaagactcc ccaaaatacc ggcgggcaac gcaaaaataa
     1801 gatgattata aaaatgggtt ataagacgtt gatatgaagt cgcaacaatg gctgcaattg
     1861 ggcgttttgt cgaataaatt gtcgcatata aatagccatt tctttgtacg gttaaatcct
     1921 tcaaatgaat cagcggagaa aacgttgctg ccgaggcctc tttgcttaac gagaaaaagg
     1981 ttttcatcac cgtatcatac acaccccatt gcaatgtcgg gtcatcagac atcacttcac
     2041 tccagaagag agggttgata accgccacat aatttccccg ctgcatatag gtcattttat
     2101 agccagagaa aaaaggcgta tcgcggtaat aatagataga aacgttaggt tcacgcttat
     2161 aatcggccgg tgcaatcgta tagccgttta caggcgctat cagcgatgag cataaaaaat
     2221 ggttatcacg aattcattaa tataaagata gccacgaata atattcaaca ttcgcttttg
     2281 atgggctgga gtgcaaactt gcccctgata gcgttcagct gcatcgctca ctaaatctgc
     2341 ctggcgaatg accagctcgg atttatccag tgccagttga gcaaaggttc ggagctgatt
     2401 attcacttct gattttgccc aaaggaaggc aatccatagc gaaagaatga tgggaaagag
     2461 aactaaaaag ataatgcccg gcaacgccag taattggtgt cgtgcacgat gactcattgc
     2521 tatcaatttc ctgcctgttt atacccggcc attgcaaggt gaaaccctgg ctaccgacgt
     2581 ctttagataa atcttacggt ataaatagca cttttgcctg atttgaaatt gatacctttt
     2641 taatcgtcac ctggatgaaa ataattcatt gatattcaaa attaattttc tacaggaata
     2701 cagaggcggc gggaagatat gcccttaatg ccactgaaaa accacataat ttgtacagca
     2761 ataaaagtca cggcctaata tggctaacaa acaatcaatt gtgatatagt tcacaaaatt
     2821 aatgaaacaa acagagtgtt tcatttttgt gttctgcaag ccccgcgata gccagagtgt
     2881 accacgcctc ccgtgaacaa cgccgcgctg tccagggcat cggctctttt cagaggaatt
     2941 gtttgatggc taccctcacc actggcgtgg ttcttcttcg ctggcaactt cttagtgccg
     3001 taatgatgtt tctggccagc acactcaaca tccgttttcg tcggtctgat tatgtcgggc
     3061 ttgcggtgat cagcagcggt ctgggcgttg tttccgcctg ttggttcgca atggggttgc
     3121 ttggcatcac aatggcggac atcaccgcta tctggcacaa catcgagtcg gtgatgatag
     3181 aagagatgaa tcagacaccg ccacaatggc caatgatttt gacctgatac gtagaa
//
LOCUS       DADLTY010000294         3179 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.298, whole genome shotgun sequence.
ACCESSION   DADLTY010000294 DADLTY010000000
VERSION     DADLTY010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.298"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="JN334_004197"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="JN334_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAY7440117.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(1772..1906)
                     /locus_tag="JN334_004198"
     CDS             complement(1772..1906)
                     /locus_tag="JN334_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7440118.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(1931..2365)
                     /locus_tag="JN334_004199"
                     /pseudo
     CDS             complement(1931..2365)
                     /locus_tag="JN334_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532398.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
     gene            complement(2552..2746)
                     /locus_tag="JN334_004200"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="JN334_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          659 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DADLTY010000295         3151 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.299, whole genome shotgun sequence.
ACCESSION   DADLTY010000295 DADLTY010000000
VERSION     DADLTY010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.299"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..134
                     /locus_tag="JN334_004201"
     CDS             <1..134
                     /locus_tag="JN334_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAY7440119.1"
                     /translation="PSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            197..418
                     /locus_tag="JN334_004202"
     CDS             197..418
                     /locus_tag="JN334_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HAY7440120.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            581..955
                     /locus_tag="JN334_004203"
     CDS             581..955
                     /locus_tag="JN334_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HAY7440121.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            1002..1376
                     /locus_tag="JN334_004204"
     CDS             1002..1376
                     /locus_tag="JN334_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAY7440122.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            1373..1864
                     /locus_tag="JN334_004205"
     CDS             1373..1864
                     /locus_tag="JN334_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440123.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            1876..2073
                     /locus_tag="JN334_004206"
     CDS             1876..2073
                     /locus_tag="JN334_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HAY7440124.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            2158..2718
                     /locus_tag="JN334_004207"
                     /pseudo
     CDS             2158..2718
                     /locus_tag="JN334_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            2790..>3151
                     /locus_tag="JN334_004208"
     CDS             2790..>3151
                     /locus_tag="JN334_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7440125.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRA"
BASE COUNT          781 a          845 c          769 g          756 t
ORIGIN      
        1 cacccagtaa ggcagaccgg cttatcaccg aacgtctggt acaggcactg ggcctggtgg
       61 atatccgggt gccggaccat ctgatagtcg gtggcaacca ggttttctcc tttgccgaac
      121 atggtctgct ttaacccgtc acaaccacat cacacctgtt ttcactttta tcttctgtct
      181 tcagaggtat cacatcatga gaatcatcac ccgtggtgaa gccatgcgta ttcaccaaca
      241 gcatcctgca tcccgtcttt ttccgttctg taccggtaag tatcgctggc acggcagtgc
      301 tgaagcgtat actggtcgtg aagtgcagga tattcccggc gttcttgccg tgtttgcaga
      361 acgccgtaag gacagttttg gcccgtatgt ccggctgatg agtgtcaccc tgaactgatg
      421 ttccgtcacc cgggaaacga gtacgaccag atgacctcag acgccgctgt ggatgaggcc
      481 attctcatca atgcatacat cttcaccgaa accggtcagc gcgccggctg acctgtcatc
      541 gttaaaccat tcttaaccac ttttcagaga ggattttatc gtgtcagaca aactctccgg
      601 gataactcat cccgatgaca atcacgaccg cccctggtgg gggcttccct gcacagtgag
      661 gccctgtttt ggcgctcgtc tggtgcaaga gggtaaccgc ctgtattacc ttgccgaccg
      721 cgccggtatc agaggccgct tcagcgacgc agatgcatac catctggacc aggcctttcc
      781 gctgctgatg aaacaactgg aactcatgct caccagcggt gaactgaatc cccgatatca
      841 gcataccgtc acgctgtatg caaaagggct gacctgcgaa gccgacaccc tcggctcctg
      901 tggttacgtt tatctggctg tttatccgac accagcaccc gcaaccacct cataaccccg
      961 aagctctcac ctttagcact accagttaca gagaacttaa gatgaaaaca ttatccgaca
     1021 cacatgtacg ggaggtatct cgctgcccgt ctcccgtcac catctggcag acactgctca
     1081 tccgactgct ggaccagcat tatggcctca cactgaatga cacaccgttt gtcgatgaac
     1141 gtgtgattga gcagcatatt gaggcaggca tttcactgtg tgatgcagtt aattttctcg
     1201 tggaaaaata cgcgctggtg cgtaccgacc agccgggatt cagcacctgt ccccgctctc
     1261 agttaataaa cagtattgat atcctccggg ctcgccgggc gaccggcctg atgacccgcg
     1321 acaattacag aacggtaaat aacattaccc tgggtaagta tccggaggcg aaatgatgaa
     1381 actggccctg acactggaag ccgacagcgt taacgtacag gcactgaaca tagggcgcat
     1441 tgtcgttgac gtcgatggta ttgagctcgc tgaactgatt aacatggtct gcgataacgg
     1501 ctactccctt cgtgttgttg atgaatctga ccagacctca gcagaatgca cgccaccatt
     1561 tgctaccctt accggcatac gctgcagtac cgcacatatc acggaaacgg acaacgcctg
     1621 gttgtactcg ctgtcacacc agaccagtga cttcggtgaa tcagaatgga ttcattttac
     1681 aggtagcgga tatctgttac gtaccgatgc gtggtcatac ccggttctgc ggcttaaacg
     1741 cctggggctg tcaaaaacgt tccgtcgtct ggtcgtcaca ctcatccggc gttatggcgt
     1801 cagtctcatt catctggatg ccagcgctga atgcctgccg ggtttaccca ctttcgactg
     1861 gtaaccagga acaacatgaa atcattaacc acggaaacag cgctggatat tctgattgcg
     1921 tggctgcagg acaatatcga ctgcggatcg ggaattatct ttgacaacga tgaggataaa
     1981 acggattcag cagcactgtt gccctgtatc gaacaggccc gggaggatgt ccgtaccctg
     2041 cgtcatctgc agcttctgca ccagaaccgg tgaagctcac tcaccagact tcattcctct
     2101 gacgccagcc tgcacagggc tggcgttttc atttatctgc aaaaaggaat atcgattatg
     2161 tcagatatca caatctcccg tccggaagtg gtcaacgggc atacggacgt tatctgctca
     2221 accagcattc gccacattct ggctgtacga aagagtacac tgttgcaaat cgacacactt
     2281 atccgacaac tggctgaaat ctcggtgctg acggaaagca tcggaggtaa aactgccccg
     2341 gactgggcca tgaaacagga ttttcgctgc ggctgctggc tgatggagaa acccgaaaca
     2401 gcaatgaaag ccattacccg gaatcttgat cgcgaaatct ggcgtgacct gatgcaacgt
     2461 tccgggatgc tgtcattaat ggatacgcag gcccgtgata catggtaccg gtcactggag
     2521 tacgataatt ttccggaaat cagcgaagcg aacattctga gcacatttga acaactgcac
     2581 cagaataagg atgaggtgtt tgagcgaggg gtgatcaacg tcttcagagg gctgaactgg
     2641 aattacaaaa ccaattgccc ctgtaaattt ggcagtaaaa ttatcgtcaa caatctggtg
     2701 agatgggacc ggtggggata atgatatggt cacccccacc ttaacagcag tacgctgtta
     2761 aggtaaatcc ctttaacgga gagaccatca tgcagaacat cacacttatt ggtatcgatc
     2821 tcggaaagca ctccttccac attcattgtc aagacaagtc tggtaaggct cttctgcgta
     2881 aaaaatttac ccgtactaaa ctgatgcagt ttctggcgac atgtccgccc tcagttgttg
     2941 taatggaagc ctgtgcgggt gcacacttta tggctcgctg tatctctgat tttgggcatg
     3001 aaactaagct gatttctcca cagttcgttc gcccgtttgt caaaagtaat aagaatgatt
     3061 ttgttgatgc agaagccata tgcgaagcgg cttcacgacc atcaatgcgt tttgtccagc
     3121 caagaactga gacccagcag gccatgaggg c
//
LOCUS       DADLTY010000296         3134 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.300, whole genome shotgun sequence.
ACCESSION   DADLTY010000296 DADLTY010000000
VERSION     DADLTY010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.300"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..68)
                     /locus_tag="JN334_004209"
     CDS             complement(<1..68)
                     /locus_tag="JN334_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440126.1"
                     /translation="MASISISCPSCSATEGVVRNGK"
     gene            260..1237
                     /locus_tag="JN334_004210"
     CDS             260..1237
                     /locus_tag="JN334_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7440127.1"
                     /translation="MILIPDDEPELSLPAASEEFLPALSGENAPVSPARAYLLSLNSH
                     RSRQTMASFLNIVAVMLGAASLESCSWGSLRRHHVMAVTELLRDTGRATATVNTYLSA
                     LKGVAKEAWMLRLMDVESFQHIRAVRNLRGSRLPSGRALPQGEIRALFAVCEADRSCL
                     GARDAAMLAVILGCGLRRSEVVSLDLRDVVTQDRALRVLGKGNKERLAYVPAGAWQRL
                     QIWIDEIRGETPGPLFTRIRRFGDVTLNRLTDQAVYHILQVRQGQAGITKCSPHDLRR
                     TFATAMLDNGEDLITVKDAMGHASVTTTQQYDRRGEQRLQDARDRLNLI"
     gene            complement(1285..1548)
                     /locus_tag="JN334_004211"
                     /pseudo
     CDS             complement(1285..1548)
                     /locus_tag="JN334_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193081.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
     gene            complement(1583..>3134)
                     /locus_tag="JN334_004212"
     CDS             complement(1583..>3134)
                     /locus_tag="JN334_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HAY7440128.1"
                     /translation="DIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAINPNSDQYL
                     EERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALIDQTSQLLP
                     RIKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLTKMAESSPG
                     LTYAKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQRFRAGGRG
                     ESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYHESDLRIE
                     EHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRPLIGGTLNI
                     KHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILD
                     WLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAI
                     VLWNTVYLERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSRRLEDGKFR
                     PLRMPGKP"
BASE COUNT          595 a          950 c          987 g          602 t
ORIGIN      
        1 cttttaccgt tacgcaccac gccttcagta gctgagcagg agggacaact gatggagatg
       61 gaagccacgg gagcacctca aaaccaccat catacactaa atcagtaagt tggcagcatc
      121 accgtaatcg tacagctaag aacaagtgct tcaagtctac ttattaaaat gtacctttga
      181 aaaagtagac tttctgcggt acgctctgac agtaagtttg cccccgattt tttacgcagc
      241 gcgccggacg gagcccgaca tgatcctgat tcccgatgac gaaccggagc tgagtctgcc
      301 cgcagccagt gaggaattcc tgccggcgct gtcaggtgag aatgccccgg tcagcccggc
      361 ccgggcctac ctgctttcac tcaattccca ccgcagccgg cagaccatgg cctcgttcct
      421 gaacatcgtc gccgttatgc tcggagctgc ctccctggag tcctgcagct ggggcagcct
      481 gcggcgccat cacgtgatgg ccgtgaccga actgctgcgc gacaccggcc gggccaccgc
      541 caccgtcaac acctatcttt cggcactcaa gggcgtggcg aaggaagcct ggatgctccg
      601 gctcatggat gtggagagct tccagcatat ccgggcggtc cgtaacctgc gcggcagtcg
      661 gctgcccagc ggtcgggcgc tgccccaggg ggagatccgc gccctgtttg ccgtctgtga
      721 agccgatcgc agctgcctcg gggcgcggga tgcagcgatg ctggcggtca ttctcggctg
      781 cggcctgcga cgatccgaag tagtgagcct ggatttgcgt gacgttgtca ctcaggaccg
      841 tgcgcttaga gtgctgggta agggaaacaa ggagcgactg gcatatgtcc cggccggtgc
      901 ctggcagcgg ctgcagatct ggatcgatga gatccggggc gagaccccgg gcccgctgtt
      961 cacacgcatc cgtcgtttcg gggatgtgac tctaaaccgg ctaaccgacc aggcggtgta
     1021 ccatatcctg caggtgcgcc agggtcaggc cggcatcacg aaatgttctc cacacgatct
     1081 gaggcgaacc ttcgccaccg cgatgctgga taacggggag gatttaatta ccgtgaagga
     1141 tgcgatgggc cacgcgagcg tcaccaccac ccagcagtat gaccgtcgcg gggagcaacg
     1201 cctgcaggac gcgcgggata gactcaacct tatttagggc taagtaaaca caaacaaggg
     1261 ggctgtaccc cctctaattt tacattaaca cgatactcga cagactttcc cagtcgtagt
     1321 cagcaaccat cgcccgatgg aggctgggat aaatgctgac agcccgacgt gaagcaaaag
     1381 gctctcgccg ctggcatggc ttaatgtcgc ggtcccctca atcactaaca caatttcagc
     1441 gctggatgtc attaccgttt gattgttcac cgcagtgaaa atactgaaat ggaaatcctc
     1501 aacaggaata ggatagcgcg cttctgcggc gtgctgttcc ggtaacatag gggagcccgc
     1561 agaattcgga aaaaatcgta cgctaaggtt ttccgggcat ccgtaagggc cgaaacttcc
     1621 cgtcttccag tctgcggctc tgccgccaga cgtaatcgcc ggttaggttg atgtgctccc
     1681 agcccagcgg cgacaggaat tgcagcagct cgccgtccac cggcttgccg gcctcgacca
     1741 acccctgggt ggcgcgttcc aggtacaccg tgttccacag cacgatagcc gccgtcacca
     1801 ggttgaggcc gctggcccgg tagcgctgct gctcgaagct ccgatccctg atttccccaa
     1861 ggcggttgaa gaacaccgcc ctggccagcg agttgcgcgc ctcacctttg ttcaggccgg
     1921 catgcacgcg gcggcgcagt tcaacacttt gcagccagtc caggatgaac agcgtgcgct
     1981 cgatccggcc cagctcgcgc agggccacgg ccagtccgtt ctggcgcggg tagctgccga
     2041 gcttgcgcag catcagcgag gcggtgacgg tgccctgctt gatcgagctg gccaggcgca
     2101 ggatgtcgtc ccagtgggca cgcacgtgct tgatgttcag ggtgccgccg atcagcgggc
     2161 gcaacgtcgg gtaggcttgc acgccctgcg gcacgtacag cttggtttcg ccgaggtcgc
     2221 ggatgcgcgg cgcgaagcgg aagcctagca ggtgcatcag ggcaaagacg tgatcggtga
     2281 agccggccgt gtcggtgtag tgctcctcga tccgcaggtc ggactcgtgg tacagcaggc
     2341 cgtcgagcac ataggtggaa tcgcggacgc cgacattcac cacgcgggtg ctgaacggcg
     2401 cgtactggtc ggagatatgg gtatagaaca gccgtcccgg ctcgctaccg tacttcgggt
     2461 tgacgtgccc ggtgctctcg ccccggccac ccgcgcggaa gcgctggcca tcggaggatg
     2521 aggtcgtgcc gtcgccccag tgggcggcaa aggcgtggcg atactggtgg ttgaccagct
     2581 cggccaaggc cgccgaatag gtttcgtcgc ggatgtgcca ggcttgcagc caggacagct
     2641 tggcgtaggt caggccgggg ctcgactcgg ccatcttggt cagcccgagg ttgatcgcat
     2701 caccgaggat tgcggacagc agcaacgtcc tgtctttggc ctcggccccg tccttcaagt
     2761 gggtgaagtg gcggctgaag cccgtccagt cgtccacgtc catcagcagt tcggtgatct
     2821 tgatgcgcgg cagtaactgg ctggtttggt cgatcagcgc ctgcgcccga tccggcaccg
     2881 ccgcatccag cggggtgatt ttcagccctg actcggtgag gatggcatcg ggcagctcgt
     2941 tgtccttggc caggcgggtg acggtggcca actgctcgtc cagcagctgc aaacgctctt
     3001 ccaggtactg gtcgctgttc gggttgatcg ccaggggcag ggcctgctcg cgcttgagtg
     3061 cggcgaactt ctcggccggc agcaggtagt cgtcgaagtc gcggaactgc cgcgagccct
     3121 tgacccagat gtcg
//
LOCUS       DADLTY010000297         3097 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.301, whole genome shotgun sequence.
ACCESSION   DADLTY010000297 DADLTY010000000
VERSION     DADLTY010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.301"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(104..1813)
                     /locus_tag="JN334_004213"
     CDS             complement(104..1813)
                     /locus_tag="JN334_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAY7440129.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2245..2964)
                     /locus_tag="JN334_004214"
     CDS             complement(2245..2964)
                     /locus_tag="JN334_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HAY7440130.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          953 a          588 c          519 g         1037 t
ORIGIN      
        1 gccaattgag gtggttgcct gaaagttggc agttacccgg tcactacacc ttgttggagt
       61 gaacatatca aaaacaggta attacacaat ttaaattaca gtctcataaa aaatgattaa
      121 atctatttat tatttcaata aacgtttttt tcatcttctt tgaaaattgt tctgctatag
      181 tatccggttc cagattgttc tcttttatta ttctacaata ctctaatatt gtttctttgt
      241 ctctatttac tacagataca aatacaccag gaataccatc tttattagtt gcggacagca
      301 tttcgaccaa tcgtcttgtg gtaaaactat agttttttag aatattacaa aaggcacgta
      361 ttgtttcggt atggccatta taaaatgcca taaaaagtcc tgatgtaccg ttgggatttt
      421 ttatccataa taattcatct atatattcct ggtgaggggg gagtattttt agtatatctc
      481 catacgccat aatggccttt gtgtgcccat tttgaagtgc cataaacaac ccatgcttta
      541 ctttcaataa ttctttaatt tgttcactac taagcatttt cttattaagc agacttccga
      601 acatacgaat actatctgca tgtccattct gcaatgcaaa aaataaccca ggtaaatcat
      661 ggctgtcttt cgcagctaat agatataatc tcttatcgga agttaacggg gttttaaaga
      721 ctatttcccc ataggctttt attgtgtcta catgtccgtt cataaaagac atgcataaac
      781 ccggcacatt gtttgcccct accgcagcaa gcaatgtaag tattgtttct tgactaagat
      841 tgcacatatt taatatctta ccatatactt gaataatgtc tgaatatccg ttttgaagtg
      901 ctatataaaa tgcaggagta ccatcagagc ttctcgtggc cagaaggttc accagtttag
      961 attgagaaat ttcaccattt ttgtattttt ccatcagtat aaataatact tctgtcagat
     1021 tctttgttat cgcatgatat ataactctgg aattgatcaa ttcatctggt aaaagtttca
     1081 ctgtgtttgt tagaggggaa ttatgcctat cttcaaatat tacctgcccg ggttgctcca
     1141 gaccataaat tttccaataa aaatcatcaa aaaaatcctt agcggataat tgactcagtt
     1201 cgaaatggct atcacaagta aatacagtac gtttatgagt aacagttcga ttaggatcga
     1261 aaaactgaac aacccatttt tcactttctt gtacgcgctt tattttcaac cctaacgcca
     1321 tttggtggtt taatgtgcat gctgataatg ttctgacaaa gtctccattt tttttcatgt
     1381 tataaaaaaa attagtaata actgttcccc actttttatt ttccacaaaa taaatataag
     1441 ctggtgattt tgaaaaatct tccgtttttt ccaaacatac attttttgca atagcatcag
     1501 gggtgctaag aagctgatag tttaccttcc catttgtttc cataaaattc atttcccaat
     1561 aggaagctag gtgacggcaa actatttgtc ttccatatat atcttttgcc actccattta
     1621 tatttacctc cacatttctc ttttctgaaa aataaggtgc tgttttattc ttaaactgaa
     1681 ttagttcact ggagtttgag accctcataa tagaacttgt tattgttcta ttatctctgt
     1741 taacagacat atctgttgat aacgaatttt ttgttgaaaa tatactagat gaaaatgttt
     1801 tatttaacgg catagaagac aaccatagag atgttataat actattagat tgcagttact
     1861 aatagtaaaa gcctccttac aaggaggtag cttattcaga atgctcctat aagatagcga
     1921 gctcgtcaac gatacagatg atctgattat attgtgcaaa aggagaacag gaaaataggc
     1981 actggaaaag atgaaggcct gatgggagca ttaactaatg caaaatccga caggttaata
     2041 tcccgaaaag caatcaaacg actcaaacag gagatgcacg ctgaaatggt ggacaactgt
     2101 tcggatttca gatgagtagc tcagcagttt atgagccgaa tgctctgtca cgggcaggaa
     2161 tcaggctgca tacgcagtgc caaatctaat cgtagttatt ctaaccagtt ttcaagctac
     2221 aaggtggtgt agctggcatc ttctctactc tatcatcaaa cgataaaatg gttcctctcg
     2281 taacatttgc tcttgccttt cacttccatc tcgatcgctt cgtagttcat tacgatagct
     2341 gacatatttc cagtcttcag gtctaacatc tgacgccgga tactccccgg gagcgatttt
     2401 gcttcttaat agattagact cgagacacat tataaattgt cgtattttat gaagtaataa
     2461 agcactatat tgcgagcatt cctgatctga ttttacatat agcgtaaact gagctcctat
     2521 ccccacacga gattgctgag aaactcgatt catatcagtt atcttccatt tatctatagg
     2581 actgtcctct gaaaataata aaccagacaa aatttggaat gccagaggaa cttgctccct
     2641 agctatacta atatgaaact tgtccccaac aaaatccccc ttactctgag gattttcacg
     2701 acatgcatga ataaacacat ctccactatg gttcacaatc tggaatcctt ggtacatgac
     2761 agaaaaagat tgacatatat cagagtaagt tggcacgttc gatgctactt tgaaatagta
     2821 cgctgggtat tgccgtattt ggttgtacaa tatattgaag ttatttttca atctttcatt
     2881 cgcagaaagc atctgcggta tttcagacct cacaacattc aaagaatcta aatttagttt
     2941 tagacagggc ttttttatgg gcatagaaaa cgtcctctat aaaatagata tattatctta
     3001 tattgattgt ttatcatctt ttataaaacg atacattata tgtaatgtat gtaagcgtaa
     3061 gggtcctctc cgaacatggt gggaaggctt agaacgc
//
LOCUS       DADLTY010000298         3089 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.302, whole genome shotgun sequence.
ACCESSION   DADLTY010000298 DADLTY010000000
VERSION     DADLTY010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.302"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            58..546
                     /locus_tag="JN334_004215"
     CDS             58..546
                     /locus_tag="JN334_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7440131.1"
                     /translation="MVSVVTGARSAEVCHLTKRDIVTLDNGLVCIDINEDGDGKSVKN
                     KHSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTPSAYSSWFNSRVLTEALGDS
                     QDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITYDLYGKGHAVGRLADVLKTA
                     LL"
     gene            958..1326
                     /locus_tag="JN334_004216"
     CDS             958..1326
                     /locus_tag="JN334_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440132.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            1757..2272
                     /locus_tag="JN334_004217"
     CDS             1757..2272
                     /locus_tag="JN334_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440133.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            2262..2762
                     /locus_tag="JN334_004218"
     CDS             2262..2762
                     /locus_tag="JN334_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440134.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGVVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
BASE COUNT          741 a          804 c          777 g          767 t
ORIGIN      
        1 tatcagttca cacgacacac ggctcacacg acctctgagg cccgtagatg ggcgtctctg
       61 gtgtctgtgg tcactggcgc tcggtctgct gaggtctgcc acctgactaa acgtgacatt
      121 gtaaccttgg acaatggact ggtctgtatc gacatcaatg aagatgggga cgggaagtct
      181 gtgaagaaca aacattcagt ccgacttgtg ccgttaaccg atggcgctta tgggtttgac
      241 ctgacgtcct tcctgtcgtg ggtagacatg cagcctgatg agggtcctct cttcgggatg
      301 actccgagcg cctactctag ctggtttaac tcaagggtcc tgactgaggc cctaggagac
      361 tctcaggatg tctctctcca cagtctgaga cattggctgg cgacccgaat gaaagagcgt
      421 ggagtcaatc tggtggacgc tcaaggcatc ttaggtcaca gcagtcagtc aattacgtat
      481 gacctgtacg ggaaaggaca cgctgtgggt aggctggctg atgtgcttaa gacagcttta
      541 ctttaagtag ggagacccaa ggtggagacc atatacggct tgatggttga gaccttgggt
      601 cactgaagtc tgactgaagt tagggagggt gataataatc ataatccctc ctgactatag
      661 gcccactggt ccgccttcgg ctgtccgact ctaagtctgt ctgtggtctt atttaaactg
      721 cgtcctaaac tccagtccct ccaaattcct atggtctcct cttgacattc ctgtttaact
      781 gtgagtcgcc tctggtctct ctttatatat acttccttaa ttcctgaggc gtctttagtc
      841 cttgacattc ggttcaacct gtgagtcaca tcctgtcgcg ctactgtatg catcaccagt
      901 gatggacttg attatcacta taggggaacc aactaattca actacaggag atactctatg
      961 gcacgaccaa tcgcaactca cgacaacacg ttcaccaaag cctacttgca gcagcactgc
     1021 ggggacctgc tgtcattcga tggtcagggc gacctgagtg gttggctgga tgatgtcctt
     1081 actggtgctg gtcgcctgaa cgaatcaatg gcaagcaata ccaagcctgt cagcccatac
     1141 ttgattctga cccaactact aacgcacgac acattaactg taagcgctgt gcaggagtcc
     1201 ctgagccgta agcgtgttgc cttgggtgaa cctatggttt caactcgcta cgctcgctac
     1261 gtctatgcta ccgtcgtcag cgccagtaaa tccgtccagt accacgccag taaagctggt
     1321 tcatgattac ggaccctaaa ctatcactat agggagacca gcctccgcct ctgagtctga
     1381 tgcgtcagaa catgcgagca tctggtcaac cgacattaag tatctctcct aagtaatcaa
     1441 ggcccgtgac cgcctctgac agacctcctg tatcgacctc cagctcgtta gcgttcttta
     1501 gtcgtctcgg ttagtagctc gtctactcac tacattggct taagtctatc accgctcacc
     1561 atctggagtc cttacagctc gtcctgacat gcggtcgcac ctttatacgt acatggagaa
     1621 catggtcact ccagaccgtc aacatttaac cattcacttt cgttaagcac tttctgatgg
     1681 gtccgtttag gatgcgtctg aatggtgcgg actccatgtg gagacagaaa gtgctttacc
     1741 aaaggagcat ctttgtatga ataccgaatt accagtaact aaccgccagt tcttaatcat
     1801 cctgctgacc aatgcagagt ccagtgtcga ttgctgtcgc tataacttag acctgctgaa
     1861 gttatcccga tggtatgccc aggcggaaga ctgggactat gagaaaatcc gggagatgta
     1921 cgcggagcct atccagtcat acctccagac acacaccgag gaagagctga agcgtggcct
     1981 gcaggacatc ttctatttca acaatcctta cacctgtgag ggtgactcag aagagatgga
     2041 ttggatagcg tcacgctctg atgccgactt gaagcacatc tctaacgcga tgatgcaggt
     2101 ggagagcaaa tggtcccgcc gccttcaggt cctaactgaa gattacgaaa acggcacctt
     2161 tgataagctg gtcccacctg atgccgaacg ccttcaggcg aaagctgacc aagaacagcg
     2221 cttcaagtcc tttgctgaag ctctggcggg aggtgcctct tatgacttct gacaataagc
     2281 aggaatacac cattcatatc ttcggtcagg acatcaccag tcaggacgga ttgtttcgac
     2341 tggatgagat acgccgggta ggcattcaga ttggtaaggt agaagataac cgtagtaccg
     2401 aggttgctcg cttcatgagg accaaggctg gtagaagtct aaccaaccca gaaaacgggg
     2461 ttgttaaaaa gattaacatg ggtcacttag ggacctcgtg gctggctgac ccagtagccg
     2521 ctatagagtt cggtcggtgg ttggaccttg ggtacggact ggcagtaacc cgagccttcg
     2581 tgtctatgac ctccagcgcc accgtgcagt ctgctgtagc gtctgctgag gacatcaagc
     2641 cggagaccaa ggaattcatc cagcgtcatg gcacaggcct ctacatcgcc ggacgtgaag
     2701 tctcacaggc tgagtattca gactatcaag ctgtgaccgc tggtcgctgt gagtggaggt
     2761 aagtaatcat ggctatgatt gaccctcgta cacctatcgg caaagcgacc cttcgttacc
     2821 gtggcctgcc cacacgtcat ctgctgtctc tgctgagact tggagtcgaa gaccctgagc
     2881 gcccttacta cagtcgagac gagctgatag ctatgctggt ggaccgtgat ttagacaatc
     2941 agttacgccg agcctttgct aagcagtcct gaccatacca aacctaattt taaacctttt
     3001 gagatgaccg atgcggggag acccgtaggc gctactgcgt ctgcgatacc gatagtctca
     3061 actctcaaga ggagaattac ctatgatgt
//
LOCUS       DADLTY010000299         3087 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.303, whole genome shotgun sequence.
ACCESSION   DADLTY010000299 DADLTY010000000
VERSION     DADLTY010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.303"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..3056
                     /locus_tag="JN334_004219"
     CDS             <1..3056
                     /locus_tag="JN334_004219"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY7440135.1"
                     /translation="MTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRY
                     KGTKTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYC
                     RRYSDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPN
                     GSIGFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKH
                     NPVLVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPA
                     WGEQITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIF
                     CGCVGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYE
                     KVSAQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSP
                     TEFTAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYIQKYLLGTESG
                     IQGEELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKD
                     DMNTSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLL
                     HDSPAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGN
                     GGKGISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVK
                     AWQALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYT
                     NVHELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFQEKA
                     LNFLTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGA
                     LTARAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGY
                     AQLAWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
BASE COUNT          754 a          846 c          843 g          644 t
ORIGIN      
        1 cgatgacctg gggcgagcaa accgacgtac cggaatctgc cgactggtat aactccagct
       61 acatcatcgc ttgggggtct aacgtaccgc agacacgtac gccggacgcc cacttcttta
      121 ccgaagtacg ctacaaaggc actaaaacca tcgccattac ccctgactac tctgaagtgg
      181 ccaaattgtg cgaccagtgg ctggcaccga aacaaggcac tgatagcgcc ctggcgatgg
      241 caatggggca tgtgatttta aaagagtttc atctcgataa tcccagcgac tactttatca
      301 actactgccg ccgctacagc gacatgccga tgctggtaat gctggagcct cgcgacgatg
      361 gtagctacgt tcccgggcgc atgatccgcg catctgacct ggtggatgga ctgggcgaaa
      421 gcaacaatcc gcagtggaaa accgtagcag ttaataccgc aggtgaattg gtagtgccga
      481 acggttcgat tggtttccgc tggggagaaa aaggcaaatg gaatctggaa tccattgccg
      541 ccggtacgga aaccgaattg tcgttaaccc tgctcggtca acatgacgct gttgcaggcg
      601 tggccttccc ctactttggc ggcattgaaa atccgcattt tcgcagcgta aaacacaatc
      661 cggtgctggt gcgccaattg cccgttaaaa acctgacgtt agtcgatggc aacacctgtc
      721 cggtggtcag cgtttatgat ttggtactgg cgaattacgg cctcgatcgc gggctggaag
      781 atgaaaacag cgcgaaagat tacgctgaaa tcaaaccgta caccccagcc tggggtgagc
      841 aaattaccgg cgtaccgcgc cagtatattg aaaccatcgc ccgcgaattt gccgataccg
      901 cccataaaac gcatgggcgc tcaatgatta tcctcggcgc aggtgttaac cactggtatc
      961 acatggacat gaactaccgc gggatgatca atatgctgat cttctgcggt tgtgtcgggc
     1021 aaagcggtgg cggctgggca cactatgtcg gccaggaaaa actgcgccca caaaccggct
     1081 ggttgccact ggcctttgcg ctcgactgga accgaccacc gcgccagatg aacagcacct
     1141 cgtttttcta caatcattcc agccaatggc gctatgaaaa agtctctgct caggagttac
     1201 tttcaccgct cgccgatgcc agtaagtaca gcggtcatct gattgatttc aacgttcgcg
     1261 ccgaacgtat gggctggcta ccttctgcgc cgcagctggg gcgtaacccg ctcgggatta
     1321 aagctgaagc cgacaaggcc ggattatccc ccacagaatt taccgcccag gcgctgaaat
     1381 cgggcgattt acgtatggcc tgcgaacaac cagatagcgg cagcaatcat ccgcgtaatt
     1441 tgtttgtctg gcgctctaac ctgcttggct cctccggcaa aggccacgag tatatacaga
     1501 agtatctgct ggggaccgaa agcgggattc agggcgagga actcggtgcc agcgacggga
     1561 tcaaaccgga agaagtcgag tggcaaactg cggcgattga aggcaagctc gacctgctgg
     1621 tgacgctcga cttccgcatg tccagtacct gcctgttctc cgatatcgtt ctgcccaccg
     1681 ccacctggta tgaaaaagac gatatgaaca cctcggatat gcatccattt attcatccgc
     1741 tttctgcggc ggtcgatccg gcctgggaat cacgcagcga ctgggaaatc tacaaaggta
     1801 ttgccaaagc attttcgcaa gtgtgcgtgg gccatcttgg caaagaaacc gacgtggtat
     1861 tacaaccact gctgcatgac tctccggcag agctctcaca gccgtgtgaa gtgctcgact
     1921 ggcgcaaagg cgaatgcgat ctgatcccgg gtaaaaccgc gccgaatatt gtggcggtgg
     1981 agcgcgacta ccctgctacg tatgaacgct ttacctcgct cgggccattg atggacaaac
     2041 ttggcaatgg cggtaaaggg atttcgtgga atacccagga tgaaatcgat ttcctcggca
     2101 aactcaatta caccaagcgt gatggcccgg cacaggggcg tccgctgatt gacaccgcca
     2161 ttgacgcttc agaagtgatt ctggcactgg caccggaaac caacggtcat gttgcagtca
     2221 aagcgtggca ggcgctgggc gagatcaccg ggcgcgaaca tacccatctg gcgctgcaca
     2281 aagaggacga gaagattcgc tttcgcgata ttcaggcgca gccgcgtaaa attatctcca
     2341 gccccacatg gtccggtctg gaaagcgatc acgtatccta taacgcggga tacaccaacg
     2401 ttcatgagtt aattccgtgg cgcacgctgt cgggacgcca gcagctctat caggatcatc
     2461 cgtggatgcg tgcttttggt gaaagcctgg tggcttatcg cccgcctatc gacacccgta
     2521 gcgtcagtga gatgcgccag ataccgccaa acggcttcca ggaaaaagca cttaacttcc
     2581 tgacgccgca ccagaaatgg ggcattcact caacctacag tgaaaacctg ctaatgctga
     2641 cgctctctcg cggtggaccg attgtctgga tcagcgaaac agatgcccga gaactgacca
     2701 ttgtcgataa cgactgggtg gaagtattta acgccaacgg cgcgctgact gcccgcgcgg
     2761 tggtcagcca acgtgtaccg ccgggcatga ccatgatgta tcacgcccag gaacgcatta
     2821 tgaatattcc tggttcggaa gtaactggca tgcgcggcgg cattcataac tcggtcaccc
     2881 gcgtttgccc gaaaccgacg catatgattg gcggttacgc gcagctggcc tggggcttta
     2941 actactacgg caccgtcggt tcgaaccgcg acgaattcat catgatccgc aagatgaaga
     3001 acgttaactg gctggatgat gaaggtcgcg atcaggtaca ggaggcgaaa aaatgaaaat
     3061 acgttcgcaa gtcggcatgg tgcttaa
//
LOCUS       DADLTY010000300         3069 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.304, whole genome shotgun sequence.
ACCESSION   DADLTY010000300 DADLTY010000000
VERSION     DADLTY010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.304"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..498
                     /locus_tag="JN334_004220"
     CDS             <1..498
                     /locus_tag="JN334_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7440136.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="JN334_004221"
     CDS             578..1273
                     /locus_tag="JN334_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7440137.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="JN334_004222"
     CDS             1302..>3069
                     /locus_tag="JN334_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7440138.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          888 a          718 c          684 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DADLTY010000301         3069 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.305, whole genome shotgun sequence.
ACCESSION   DADLTY010000301 DADLTY010000000
VERSION     DADLTY010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.305"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..666)
                     /gene="ipaH9.8"
                     /locus_tag="JN334_004223"
     CDS             complement(<1..666)
                     /gene="ipaH9.8"
                     /locus_tag="JN334_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HAY7440139.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQ"
     gene            complement(928..1023)
                     /locus_tag="JN334_004224"
                     /pseudo
     CDS             complement(928..1023)
                     /locus_tag="JN334_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1059..1364)
                     /gene="ccdB"
                     /locus_tag="JN334_004225"
     CDS             complement(1059..1364)
                     /gene="ccdB"
                     /locus_tag="JN334_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HAY7440140.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1366..1584)
                     /gene="ccdA"
                     /locus_tag="JN334_004226"
     CDS             complement(1366..1584)
                     /gene="ccdA"
                     /locus_tag="JN334_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HAY7440141.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2120..3069)
                     /locus_tag="JN334_004227"
                     /pseudo
     CDS             complement(2120..3069)
                     /locus_tag="JN334_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          765 a          745 c          740 g          819 t
ORIGIN      
        1 ttgcagagca tgggatgata atgggttatc actaatatgt attgaacatt cattcctcag
       61 attaagaatg ctttccggga tatgacttat ctgatttcta tcaaaaaaat actctctgac
      121 aacgggatta tttccctcag aaaatgctgg tagctcagtg aggaaattac gggtcgctct
      181 gagattttta agagcctgtg ggagcgatgg taaggagact atttcgttat agctgatatt
      241 catagtcaat aacgaatcgg gtaaagctgg caggttttcc agctcattgt gttgtacctg
      301 aagtgactcc agcgcaggag gtagcacggg caattctgat aatttattgc tggcggaata
      361 taattttttt agcgtaacag gcagttcagg taggttagtt aattgattat atgatacatt
      421 gagcagcgtt atctgagctg gtaagttgtc aggtagcgag gacagattta aacggtccag
      481 tcgaagttca tcggaattat tgataagaca ttctttaagt cgggagacag cctcatcacg
      541 ctcttcaccg gggagcgctt gtttttccca tttatcccat gctgaaaagt aatcagcata
      601 tgtaccggaa atagtgttat aaaaagaatt ttggggcaat gaaaagttat tatttatcgg
      661 taacatgatg ggcatccagt taaagccggt ggacaaatgg ctttgttaaa agaaataagt
      721 aggagttttt atgaagttca ttccatgaaa tgaacttcag cactttttta ttataataag
      781 gataaacaaa attggctgta aaaaaacgat actgtttcag ttaaccgtga atggcctgtt
      841 tgcaccgtat tgttcttgtt tttcggttag ttaaacatcg tacttcatat ttgaacggta
      901 agcggctcgt cagaaccgta ttgatattta ctgagaggtc agatcaactt tccaaggcaa
      961 cagatcgcgt acccggtttg ccggccagtc ctggatatgc tcaatgacgt aacgcagcca
     1021 ttttgtcaac gccacgattt ttgaccctag cctgacattt atattcccca gaacatcagg
     1081 ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata
     1141 acgaagatcg gcacactggc catatcagtg gtcatcatgc gccagctttc atccccgaca
     1201 tgcaccaccg ggtaaagctc acgggagact ttatctgaca gcaggcgggc actggccagg
     1261 gggatcacca tccgtcgtcc gggcgtgtca ataatgtcac tctgtacatc cacaaacaga
     1321 cgataacgac tctctctttt ataggcgtaa accttaaact gcatttcacc agtccctgtt
     1381 ctcatcggca aaagagccgt tcatttcaat aaaacgggcg atctcagcca tcccctgctg
     1441 attttcagcc tgccagcgtt ccgcacgcag acggcgggcc tctttttgca tggccgtatt
     1501 taccaggcct gagatattca cgtttgcgga tttaagtaac tgatagctgt cactgtctat
     1561 agtgacagta atacgctgct tcatgataag ccctcaaaaa catacttatg atatgtatat
     1621 gagtgtatat taatggtaca aaccatcaat atccataaaa agtacaccat taattcgcta
     1681 taagttttta atacaatttt ttctgctgcg atttttttat actataatta gcatacttat
     1741 aacaccgtaa gtaaattaca tgaaagcagg cacagcgtga tccgattttt ctgcgtataa
     1801 cgattttccg gaagtgtaat acggttgcgc cccgggccgg gcaccctttt ctgttgttgt
     1861 gtacgggccc acagagaatg gtaccggagg cgggctcaga agtactgagt cgggtgatcg
     1921 cgtcaggccg cgaagtactg acacgggtat tggtgactgg tgatgaagta ctgccccggg
     1981 tatgatttca ggctgattca ggttctgatt ctctgtcagt ccgcgtggtt gctggcctgg
     2041 ctgatttcct gttgtcaggc gttcaacaac cgactgcagg ttatagcccg gttcacgaac
     2101 acctgacggc aggtatcact cacccggtga aggtaaatcc ctcaagaggc agcaactctg
     2161 ccagtcgctc ctccggccat tccggcagac gcgtcaggac gtccgtcagc caggcatgtg
     2221 gctccagacc attacgtttt gctgtttcca gaaggctcat tatctgtgcc gcccggtttc
     2281 cggccatcag tgaaccggcg aacagccacg ctttccgtcc cataacaacc gggcgaatgg
     2341 cacgctcgca gatattgttg tccaggggca cagctccgtc acccaggaag cagctcagtt
     2401 catcgcgccg gttcagaata tagttaatcg ccttatttaa tggcccgtcg ggcggacagc
     2461 acggctcctg ctcttcaagc caggcaaaca ggtcattcac tatcggccgg gagtaccgtt
     2521 atcgccactg ccgtattttt tccacagggc gctcacggat gagtttctca atgcggtaaa
     2581 gacccgcgat ttttctcagc tccacgcctg cacggggctc cctgctggcc ttatacagat
     2641 ttgcgaagcc gcgccctgcg tgactccagc aacaggctga tgtgatgccg gggtgattat
     2701 ccgccagaga tttgtacacg ctgtatccgt cactcaccag cgtactgcca caccagcact
     2761 gcagccaggt ctcagggtat ctgagggccc gtccggtctg cgagtcaaaa cagacgaccg
     2821 gcggaccgct tctttcaccg ctgacgtaag cacacagcca gccggagcag gatttaccgc
     2881 ctttccgggt atcaaggagc ctcagggacg tttcatctgc atgaataaca tcccgtgtca
     2941 gaagctcatg gcgtagcagt ttcgccagcg gggccagtgc agctccggca acaccgacca
     3001 tatccgccat tgtactgacg gggagctcca cacccattcg ggagaaaatg tgctgctggc
     3061 gatacaggg
//
LOCUS       DADLTY010000302         3065 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.306, whole genome shotgun sequence.
ACCESSION   DADLTY010000302 DADLTY010000000
VERSION     DADLTY010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.306"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..197
                     /locus_tag="JN334_004228"
     CDS             <1..197
                     /locus_tag="JN334_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAY7440142.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
     gene            236..1039
                     /gene="agaC"
                     /locus_tag="JN334_004229"
     CDS             236..1039
                     /gene="agaC"
                     /locus_tag="JN334_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HAY7440143.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1029..1820
                     /gene="agaD"
                     /locus_tag="JN334_004230"
     CDS             1029..1820
                     /gene="agaD"
                     /locus_tag="JN334_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HAY7440144.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1821..2576
                     /locus_tag="JN334_004231"
     CDS             1821..2576
                     /locus_tag="JN334_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HAY7440145.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
     gene            2977..>3065
                     /locus_tag="JN334_004232"
     CDS             2977..>3065
                     /locus_tag="JN334_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7440146.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVF"
BASE COUNT          797 a          683 c          727 g          858 t
ORIGIN      
        1 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
       61 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac
      121 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      181 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      241 tgaaataacc ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga
      301 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc
      361 tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc
      421 cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg gtctgatgac
      481 caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc
      541 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
      601 gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg
      661 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
      721 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt
      781 tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt
      841 caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt
      901 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
      961 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
     1021 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
     1081 cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
     1141 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga
     1201 aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat
     1261 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac
     1321 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta
     1381 tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt
     1441 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
     1501 tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa
     1561 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
     1621 tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata
     1681 aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
     1741 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt
     1801 ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta
     1861 aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac
     1921 gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg
     1981 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
     2041 aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
     2101 cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac
     2161 gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg
     2221 taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa
     2281 acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc
     2341 aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc
     2401 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga
     2461 ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
     2521 tcccggcttc atttttatgg ctgcacagta actttatttg tttaattaat acctaaccag
     2581 atgaaaaatt tgctcaaaat ttttaaatta acttattcaa cagtcacgca ttatattaaa
     2641 taacatttga ctgggttgaa cataacgcca atagcaaaag gagttatgtt caacaattta
     2701 attttagata tacatataac ttgttctatt aataaatcac ctcgtattct gactcggccc
     2761 ctggcgcagc aaagtcaggc atttatactc tttttaacac atagaaaatt catatcaata
     2821 taataaaaac ataaaatatt tgcatataca tataatctta aagtttagtc tatttaatgt
     2881 tcaatgaaat atttctacct gtataatctt taaagatgtt gaacatatat tcacattaaa
     2941 tatgattatg tacttgttac aaggataagg ttataaatga ataaagttac aaaaacagct
     3001 attgtgggct tacttgcgct tttcgcaggt aatgccgctg caaccgatgg tgaaatagtt
     3061 tttga
//
LOCUS       DADLTY010000303         3012 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.307, whole genome shotgun sequence.
ACCESSION   DADLTY010000303 DADLTY010000000
VERSION     DADLTY010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.307"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            2..2917
                     /locus_tag="JN334_004233"
     rRNA            2..2917
                     /locus_tag="JN334_004233"
                     /product="23S ribosomal RNA"
BASE COUNT          799 a          657 c          941 g          615 t
ORIGIN      
        1 aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga tgaaggacgt
       61 gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc gatttccgaa
      121 tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg ttaatgaggc
      181 gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc
      241 cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga
      301 agcgtctgga aaggcgtgcg atacagggtg acagccccgt acacaaaaat gcacatactg
      361 tgagctcgat gagtagggcg ggacacgtgg tatcctgtct gaatatgggg ggaccatcct
      421 ccaaggctaa atactcctga ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa
      481 agaaccccgg cgaggggagt gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag
      541 cacgcttagg cgtgtgactg cgtacctttt gtataatggg tcagcgactt atattctgta
      601 gcaaggttaa ccgaataggg gagccgaagg gaaaccgagt cttaactggg cgttaagttg
      661 cagggtatag acccgaaacc cggtgatcta gccatgggca ggttgaaggt tgggtaacac
      721 taactggagg accgaaccga ctaatgttga aaaattagcg gatgacttgt ggctgggggt
      781 gaaaggccaa tcaaaccggg agatagctgg ttctccccga aagctattta ggtagcgcct
      841 cgtgaattca tctccggggg tagagcactg tttcggcaag ggggtcatcc cgacttacca
      901 acccgatgca aactgcgaat accggagaat gttatcacgg gagacacacg gcgggtgcta
      961 acgtccgtcg tgaagaggga aacaacccag accgccagct aaggtcccaa agtcatggtt
     1021 aagtgggaaa cgatgtggga aggcccagac agccaggatg ttggcttaga agcagccatc
     1081 atttaaagaa agcgtaatag ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg
     1141 ctaaaccatg caccgaagct gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt
     1201 ctgtaagcct gtgaaggtgt gctgtgaggt atgctggagg tatcagaagt gcgaatgctg
     1261 acataagtaa cgataaagcg ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc
     1321 caacgttaat cggggcaggg tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat
     1381 gggaaacagg ttaatattcc tgtacttggt gttactgcga aggggggacg gagaaggcta
     1441 tgttggccgg gcgacggttg tcccggttta agcgtgtagg ctggttttcc aggcaaatcc
     1501 ggaaaatcaa ggccgaggcg tgatgacgag gcactacggt gctgaagcaa caaatgccct
     1561 gcttccagga aaagcctcta agcatcaggt aacatcaaat cgtaccccaa accgacacag
     1621 gtggtcaggt agagaatacc aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa
     1681 tggtgccgta acttcgggag aaggcacgct gatacgtagg tgaagcgact tgctcgtgga
     1741 gctgaaatca gtcgaagata ccagctggct gcaactgttt attaaaaaca cagcactgtg
     1801 caaacacgaa agtggacgta tacggtgtga cgcctgcccg gtgccggaag gttaattgat
     1861 ggggttagcg caagcgaagc tcttgatcga agccccggta aacggcggcc gtaactataa
     1921 cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acctgcacga atggcgtaat
     1981 gatggccagg ctgtctccac ccgagactca gtgaaattga actcgctgtg aagatgcagt
     2041 gtacccgcgg caagacggaa agaccccgtg aacctttact atagcttgac actgaacatt
     2101 gagccttgat gtgtaggata ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag
     2161 ccgaccttga aataccaccc tttaatgttt gatgttctaa cgtggacccg tgatccgggt
     2221 tgcggacagt gtctggtggg tagtttgact ggggcggtct cctcctaaag agtaacggag
     2281 gagcacgaag gttggctaat cctggtcgga catcaggagg ttagtgcaat ggcataagcc
     2341 agcttgactg cgagcgtgac ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg
     2401 gttctgaatg gaagggccat cgctcaacgg ataaaaggta ctccggggat aacaggctga
     2461 taccgcccaa gagttcatat cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat
     2521 cctggggctg aagtaggtcc caagggtatg gctgttcgcc atttaaagtg gtacgcgagc
     2581 tgggtttaga acgtcgtgag acagttcggt ccctatctgc cgtgggcgct ggagaactga
     2641 ggggggctgc tcctagtacg agaggaccgg agtggacgca tcactggtgt tcgggttgtc
     2701 atgccaatgg cactgcccgg tagctaaatg cggaagagat aagtgctgaa agcatctaag
     2761 cacgaaactt gccccgagat gagttctccc tgacccttta agggtcctga aggaacgttg
     2821 aagacgacga cgttgatagg ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta
     2881 ctaatgaacc gtgaggctta accttacaac gccgaagatg ttttggcgga ttgagagaag
     2941 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaattag
     3001 cctggcggcc gt
//
LOCUS       DADLTY010000304         3001 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.308, whole genome shotgun sequence.
ACCESSION   DADLTY010000304 DADLTY010000000
VERSION     DADLTY010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.308"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..2440)
                     /locus_tag="JN334_004234"
     CDS             complement(<1..2440)
                     /locus_tag="JN334_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HAY7440147.1"
                     /translation="MLMKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSL
                     AAVTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTG
                     GQWIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQW
                     VHGGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTT
                     INKNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRD
                     GWQVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAF
                     SVVEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLA
                     DSGAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGT
                     LAGTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNT
                     TLTQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATW
                     NIPDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQ
                     NNADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGN
                     KLQAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGV
                     SGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAG
                     VYGAAGHSSVDVKDD"
     gene            complement(2768..>3001)
                     /locus_tag="JN334_004235"
     CDS             complement(2768..>3001)
                     /locus_tag="JN334_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440148.1"
                     /translation="MTALPDHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTA
                     ESVCVASVVRTALRAVRDTVVSVARAVWNWIFF"
BASE COUNT          585 a          886 c          809 g          721 t
ORIGIN      
        1 catcatcctt aacatcaacg gaagaatggc cagcagcacc atataccccc gcggtcacag
       61 acataccggc aacctctgtt ctgagcaggt caccctccag acggacgaag ccatagctgc
      121 cgctgctttc cggcgtggct ccacgggcaa taccgccgtt gttatcgtga ccgagatgac
      181 cgccctgaat gctgagacgg acgctgttat tttcaccgct tacaccggtc tgatggctgc
      241 gggagcctgc cagaatccgg tcatagtcca ttgcctgtgt cagcatggag gcatacaggg
      301 ggacttctgc acgataagca ttttcactgc gcagatacca gctctcatca ctgtcccggt
      361 tgagggagta gttaaaggca ccggcctgca gcttattccc ctggataaag gccccttcct
      421 ccgtggtggc accgttaatg gcttccacca cctgaatacc cttaccgctg gtcgccagcc
      481 ccgacgcact gttgccggcg ttcaccaggt tcaggatggt ttttccggtt gccctgccac
      541 cgtcaatgac cagtctgtca gcattgttct gtgccatatc cgggcgtaca cgcaggctga
      601 tggtgccatt ctgtccgttc aggtttttca ctttcagggt tgccggtacg aacttccctg
      661 tgcgggtgga ggtgaaatga atctgtccgg catggctgag gtcatccacc accgactgca
      721 ccgtggcgtt atcgggaata ttccaggtgg caccagaggc gagagtgaca ttcgtggggt
      781 caatggcacc gttcagcaca gtgctgccgg tcagcttcag ggctgtaccg cgctgagcaa
      841 tgacatccgt ggtgacggta ctgtcgttca gcgtcagcgt gccttcattc aggttgacgg
      901 ctttctgggt gagtgtggtg ttgctgacag tgagtgtgcc acttcctgat ttttccacgc
      961 tgccgttacc ggtgagagcg cctccctgca ggagtgcatc gccttcacgg atggtcaggg
     1021 tatcgttgtt caccgtcttc ccggaaaggg taagtatggc gccgttattc agcgtggtgg
     1081 tgcccgccag tgtgccgccc ctggcggtga acagtccgcc atttaccgtg gtatccgtgg
     1141 ccgtatcacc ggcgttcagc gtgaatgaac tgcctttttc cagcatcagg gcatccgcct
     1201 gaccgcctcc gatactgaat gcctttccgt cgctccgggt accactgaca gcggcaccgg
     1261 aatcggccag cagtacaccg ccatttccca tggatacggt ggtggcggtg ccaccgttgc
     1321 ggacatccag cgttccgcca tcatccacgc gggtattagt ggctgtgtgt ccggtcagca
     1381 catccaggcg tccgccattt tccagtacga cattatcagc tttaccctcc acaacagaga
     1441 atgctcccag gcggtttgtg ccggtgacgg ttgcagctgt actggtaacc agtgcaccgc
     1501 ccgtgttctg ggtgacatca gacgctttac cgccggcatt cacctgcagc tttcctttct
     1561 ggttgatggt ggtatgcgcg gcagttcctc cttccttaat cacctgccag ccatcacggt
     1621 ttatcagcgt ctctgttgcc gtgccaccgt tgtgtacata ctggttaccc ccctccagtc
     1681 gggtgttatg tgcctctccg tgtaccgtct ggtcaccacc ggcgtaaatg agggtgtccc
     1741 gtgccatccc cgaagaccag ataacctgcc ggccattttt attgatggtg gtggattcag
     1801 ccgtccctcc gaccatctga ccgctggaca cattttcaga ctccggacca ccttctgcac
     1861 cggtgttgac gatggttccg gttgccagtc cgccatgttt tatggtctgg taaccatcct
     1921 ggttaataat tgtaccggca gctttccctc ccccgtgtac ccactgctca cctctgttat
     1981 ccagcgtggt gttcaccgcc agtccgttaa ggctctgccc tccgccatca cgaataaccg
     2041 tatcactggc agttcctcct gcctgcacaa tctgacgacc atttgcggtg acagtggtgt
     2101 ttctgcctgt gccacccgct tttatccatt gcccgccggt gttttcgtca ctgtccggcc
     2161 ccagctcaag cccggtactg acagtcacgc catcagctgt tccggataca aactggttgt
     2221 catggtttac cagtgttccg ccattcactg tttcacccgg gtgcacaacg atgtcagcag
     2281 ccagcaccgg gagtgacgtg accgcggcaa gagacagtgc aaccgccaca ccgccacgtt
     2341 taccctgtgc gcgggccagt tcggaggcaa ccacgaaagc gcccgtaatg tgattccata
     2401 ccagcctgta gcaggtattc agatgtcgtt tcatcagcat ttccttaatc agagtgaggg
     2461 tgaataaaaa agccggtatc cacagatgtg gataccggca ggtaaagggt tacagtgccg
     2521 gaggtccggc tgacggaagt ttctgacgtg tggctctgtt gttccctgaa tctcatgtct
     2581 gtgtgtatct gcgcacgttg ctaacaggag ttattattag cataacgatc gatattaacg
     2641 atcggtaaaa taggtatcga tcgttttatt ctattatcag tctattgcac ggacaagaga
     2701 gacagtaacg aaaacagcgc ttttgtgagc agactcagag gcagggagga catcatccac
     2761 aacaggttca gaagaagatc cagttccata ccgcgcgggc aacagagacc acggtgtcac
     2821 gaacagcgcg cagggccgta cggacaacag aggcaacaca gacgctctcc gctgtgtcaa
     2881 atatccggtc caccgcaccg gtaaactgtt cacgggcctg agagcggaca gactccgtgc
     2941 gcagctcgtc ctgcagtcgg gtcatcaggg ggctggcggc atggtcggga agcgctgtca
     3001 t
//
LOCUS       DADLTY010000305         2951 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.309, whole genome shotgun sequence.
ACCESSION   DADLTY010000305 DADLTY010000000
VERSION     DADLTY010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.309"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="JN334_004236"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="JN334_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HAY7440149.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="JN334_004237"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="JN334_004237"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAY7440150.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="JN334_004238"
     CDS             2603..>2951
                     /locus_tag="JN334_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY7440151.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          701 c          779 g          813 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DADLTY010000306         2934 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.310, whole genome shotgun sequence.
ACCESSION   DADLTY010000306 DADLTY010000000
VERSION     DADLTY010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.310"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            95..2248
                     /locus_tag="JN334_004239"
     CDS             95..2248
                     /locus_tag="JN334_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440152.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..>2934
                     /locus_tag="JN334_004240"
     CDS             2301..>2934
                     /locus_tag="JN334_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440153.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYA"
BASE COUNT         1071 a          529 c          538 g          796 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccg
//
LOCUS       DADLTY010000307         2932 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.311, whole genome shotgun sequence.
ACCESSION   DADLTY010000307 DADLTY010000000
VERSION     DADLTY010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.311"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="JN334_004241"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="JN334_004241"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAY7440154.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /locus_tag="JN334_004242"
     CDS             643..1602
                     /locus_tag="JN334_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7440155.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="JN334_004243"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="JN334_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HAY7440156.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2753..>2932
                     /locus_tag="JN334_004244"
     CDS             2753..>2932
                     /locus_tag="JN334_004244"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HAY7440157.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASGELW"
BASE COUNT          659 a          800 c          806 g          667 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaactgt gg
//
LOCUS       DADLTY010000308         2894 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.312, whole genome shotgun sequence.
ACCESSION   DADLTY010000308 DADLTY010000000
VERSION     DADLTY010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.312"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..308)
                     /locus_tag="JN334_004245"
     CDS             complement(<1..308)
                     /locus_tag="JN334_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAY7440158.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     "
     gene            complement(355..1074)
                     /gene="yehT"
                     /locus_tag="JN334_004246"
     CDS             complement(355..1074)
                     /gene="yehT"
                     /locus_tag="JN334_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HAY7440159.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="JN334_004247"
     CDS             complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="JN334_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HAY7440160.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          748 a          774 c          657 g          715 t
ORIGIN      
        1 tcatcggttt tcagcgaaaa cgcgatgcgt aattttttca gcacgatgtt gttattaatg
       61 cgacgttccg gctccagtgc cggagcagac tcatccttgc cgcgtttttc ataaatcagg
      121 ccattgagga atgacgacaa aacaatgtcc ggacaacgct gaaaaccctc ttcgtcttct
      181 ttacgtagcc agacggcgat ctgttccgcg gtggcttcga cattatccag cgccagaata
      241 cgcaccaggt cattattatt ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta
      301 cttatcatga ggccttcagg tgttgatgag gcaaaaagcc attttagcag tcttttacag
      361 gccaatcgcc tcttttaagc ttttcagata acggcggctg accggcacgg ttaagccatt
      421 acgcaaaatc aactcggcct ggccgttatc ttccagacga atctcctgta aatgcgcgag
      481 gttaaccaga tactgacgat ggcagcgcag tagtggtgta cgactttcca aggtacgtaa
      541 tgtcaattcg gtaaagccct ctttcccttc gtggctggta acgtagacac cgctcatccg
      601 actgctgaca aatgccacat ctttcatttg cagcaaataa atccgactat gccccgtaca
      661 agggataaat ttcagcgcct gttgattttc cggtaacagc gaaacatcct gcttgctgcg
      721 ctcctgacgc aatcgcgcca gcgttttctc cagtcgcgct tcatcaattg gcttcagcag
      781 ataatcaaag gcatgttctt caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa
      841 aacaatatac gggcgatgtt ccgggtcaag catccccacc atttccagac cactgatgcg
      901 cggcatctgg atatcgagaa acagcacatc cgggcgcagt ttatgcaccg cgccgatccc
      961 ttccacggcg tttgaacact ctccaacgat ttcaatatcg ctctgctcct gcaaaaatat
     1021 acgcaggttc tcccgtgcta acggttcatc atcgacaatt aagactttaa tcatgcctcg
     1081 tccctccatg gtagtcgtaa cgttattcgg gtgtaactat caggctcaca ggcgacgctt
     1141 attccatagt catcgccaaa ccgttcacgt aaacgcttat ccaccagatt catccccagc
     1201 ccactggcat tggttaccgg ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa
     1261 tgttgcccct cacgtcgggc gctgattgcc actcgccctg tatccagcag ttgtgatgtc
     1321 ccatgtttaa tggcgttttc cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg
     1381 gataattctt gcggaatagc aatgttgacc tgcaaccgcg actggaagcg cgccttttca
     1441 atttgcagat aagcgttcac atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc
     1501 cgctttaagt ttttgcggaa aaaagtggaa agatactgca ccagctggct ggcctgttcg
     1561 ctgtcgcggc ggatcaccgc tttaatggtg ttaagcgcat taaacaaaaa atgaggattc
     1621 acctgggcgt gaagcagttt gatttctgac tgggtgagca tcgctttttg ccgctcatat
     1681 tgccctgcaa gaatctgcgc cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt
     1741 attgaactga ataaccggtt tttggcttca tacaatttga tggtgcccat cacgcgctga
     1801 ttttcgccac gcaacggaat taccagcgtc gaccccagtt tgcattgcgg atgcaaagag
     1861 caacgataag gtacttcgtt gccatcagcg tagaccactt caccggtttc aatcgctttt
     1921 aaggtgtaag tcgaagaaat cggtttgccg ggtaaatggt ggtcgtcacc aattccggta
     1981 aaggccagca atttctctcg atcggtaatc gcgactgcac caatatccag ctcctgatac
     2041 agtacctgag ccactttcat gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc
     2101 gtcgaggctg ccactttcag cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc
     2161 gcgcgtttat cgagcaatat acgcataaac agcgccgcgc cgacggtatt ggtgaccatc
     2221 attggcgcag caatattact caccagacgc accgcatctt cataaggtcg ggcgatcgca
     2281 aggatgatca gcatttgcac catttcagcg acgaacgtga cggcaccggc ggtaatgggg
     2341 ttaaagactt tatcagtgcg cccgcgacgg atcaggatgc tgtgtaccag gccgccgagc
     2401 aatccttcaa cgatggttga gatcatgcaa cttagcgcgg tcatgccccc catcgaatat
     2461 cgatgtaagc cgccggtcag accaaccagc ccaccgacga ccggaccgcc gagtaagccg
     2521 cccattaccg cgcctatcgc acgggtattg gcaatagaat cgtcaatgtg caacccaaac
     2581 caggtgccca tgatgcagaa gatggaaaag acgatgtagc agagaaattt atgcggcaga
     2641 cgaaccgtga cctgcattaa cggtatgaat aatggcgttt tactcattaa ccacgcaatg
     2701 actaaaaaaa cgcacatctg ctgaagcagc agcaacacca gattaaaatc gtacataccc
     2761 gcaaaccaca cttcccttta aaacgcgtaa catacattgc ctgcgtttaa ctttctttga
     2821 actcttgcag aaaaatgaga attcgtgagt acgatcactc aaaatcgcct ggcaaaaata
     2881 aaatcaccct atag
//
LOCUS       DADLTY010000309         2888 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.313, whole genome shotgun sequence.
ACCESSION   DADLTY010000309 DADLTY010000000
VERSION     DADLTY010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.313"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            9..779
                     /gene="lsrF"
                     /locus_tag="JN334_004248"
     CDS             9..779
                     /gene="lsrF"
                     /locus_tag="JN334_004248"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HAY7440161.1"
                     /translation="MQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAPLF
                     EHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNSCA
                     VAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAA
                     EMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVDMG
                     RNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            803..1093
                     /gene="lsrG"
                     /locus_tag="JN334_004249"
     CDS             803..1093
                     /gene="lsrG"
                     /locus_tag="JN334_004249"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HAY7440162.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1133..1891
                     /gene="tam"
                     /locus_tag="JN334_004250"
     CDS             1133..1891
                     /gene="tam"
                     /locus_tag="JN334_004250"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HAY7440163.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1895..2809)
                     /locus_tag="JN334_004251"
     CDS             complement(1895..2809)
                     /locus_tag="JN334_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440164.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          760 a          667 c          771 g          690 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg ggccgactat cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
      721 ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac
      781 tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga
      841 caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga
      901 aggtaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta
      961 cgaagcctat aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac
     1021 ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg
     1081 gttgatgccg tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aaatgtctga
     1141 ctggaacccc tctttatatc tgcacttttc cgctgaacga tcgcgtccgg cggtggagct
     1201 gcttgccaga gtgccgctgg agaatgtcga atatgttgcc gatcttggct gtggcccagg
     1261 taacagcacc gccctgctgc atcaacgttg gcctgcggcc aggataacag gcatcgactc
     1321 gtctccggcg atgattgctg aagcgcgcag tgctttgcca gactgccagt tcgtggaagc
     1381 agacatccgc aactggcaac cggaacaggc actcgatctg atttttgcta atgcctcact
     1441 gcaatggctg cccgaccact acgaattgtt tcctcacttg gtttctttac ttaatccgca
     1501 gggcgtgctg gcagtacaga tgccagataa ctggctggag ccgacccatg tgctcatgcg
     1561 cgaagttgct tgggaacaaa actacccgga tcgcgggcgt gagccgttgg ctggcgttca
     1621 tgcttactac gatattttga gcgaagccgg atgtgaggtc gatatctggc gaaccactta
     1681 ctatcaccag atgccgtcgc accaggcgat tatcgattgg gtgaccgcca ctggattacg
     1741 tccgtggtta caggatctga ccgagagcga acagcagctt tttcttacgc gctaccatca
     1801 gatgctggaa gagcagtatc cactgcaaga gaacggacag atactgctgg catttccgcg
     1861 tctgtttatt gttgcccgcc gtacggagta attatcacgt cagctggtaa tgacgatcgg
     1921 gaagaatttt tgctggaatt tctgcttcat cgttcatctg taacaggtca atttcaatag
     1981 cgttgcagat ggcatccagc ggtaaatcat tgttttcagt accgaacgga tcttccagtt
     2041 cttccgccag acaatccagc gaaataaaag tgtaggaaat cagcacagag ataaaaggcg
     2101 tcatgtaatg caggtccacg accagcgcga acggcagcat gatacaaaac agataaacgg
     2161 tacgatgcaa aatcagggtg taggcaaagg gaattggcgt gtaggcaatg cgctcacagc
     2221 ctgccagaac cgctgaaata tcattaagcc gatcgttgag gctaataaac aggatatctg
     2281 aaagctgtcc attgcggcgc tgaaccgcca accattctcc cattattaac aagatacggt
     2341 tagctggaga gttcgaagcc agtacacgct gaagatctcc agtcttgaga taatgagcca
     2401 gcacttccgc ctgtggctgt ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt
     2461 gcagcctggc aaactccctt acacttgccg aatccggcaa tgtcgttttt acctctcgca
     2521 gtaacgaccg tgaggcaatc atcaactgcc cccaaagttt tcgcgcttca acgtaacggg
     2581 cgtacccggc attattacga aaaccaagaa aaatggcgat ggcgacaccg agaatgctga
     2641 acggtgcgag ggtgaatttg atgcccagat gcgtgtacca gggcagcatg aatataacag
     2701 caatagaaaa gagaaaattg agtagtaagc gcgaggatat cttggataat actgagccgt
     2761 gccagacaaa aatacggcgc agccagtgtt gttgtggacg aacaatcatg gttatcttca
     2821 ggcgtggaaa agtcgcccta ttaaacgtga ttacgatcac attctcaaga cgctacttac
     2881 aaattacc
//
LOCUS       DADLTY010000310         2838 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.314, whole genome shotgun sequence.
ACCESSION   DADLTY010000310 DADLTY010000000
VERSION     DADLTY010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.314"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..1079)
                     /locus_tag="JN334_004252"
     CDS             complement(<1..1079)
                     /locus_tag="JN334_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAY7440165.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKET"
     gene            complement(1658..2335)
                     /gene="bioD"
                     /locus_tag="JN334_004253"
     CDS             complement(1658..2335)
                     /gene="bioD"
                     /locus_tag="JN334_004253"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY7440166.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(2328..>2838)
                     /gene="bioC"
                     /locus_tag="JN334_004254"
     CDS             complement(2328..>2838)
                     /gene="bioC"
                     /locus_tag="JN334_004254"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAY7440167.1"
                     /translation="KDAADHYLAGDIESLPLATATFDLAWSNLAVQWCGNLSTALREL
                     YRVVRPGGVVAFTTLVQGSLPELHQARQAVDERPHANRFLPPDEIEESLNGVRYQHHI
                     QLITLWFDDALSAMRSLKGIGATHLHEGRDPRILTRSQLQRLQLAWPQQQGRYPLTYH
                     LFLGVIARE"
BASE COUNT          681 a          766 c          697 g          694 t
ORIGIN      
        1 gtctctttac gcgcccggtc accgcgatac atgccgccaa tttgtggaat ggtgacgtga
       61 gattcatcga cgaccagcag cccatcggca ggcaggtaat caaacagcgt cggcggtggc
      121 tcacccggtc cacgaccgga gaggaagcgc gagtagtttt caatccccga acagtagccc
      181 agctcgtcca tcatctccag atcaaactgg gtacgctggg tcagccgctg ctcttccagc
      241 agtttgttgt tttccaacag cactttgcgt ctggcggcca gttcttcttt gatctcctcc
      301 atcgcctgta cgatgcgctc gcgcggtgtg acgtagtgcg ttttcgggta gatggtaaaa
      361 cgtggaatag tggaaacaat ctgcccggtc agcgggtcaa ataacgacaa tcgttccact
      421 tcctcgtcaa acagttccac gcgaagtgca atgtcatccg attctgccgg gaagatatct
      481 atcacctcgc cacgaacgcg gaaagtacca cgctggaatg cttgatcatt acgagcgtat
      541 tgcagctccg ccagtcggcg cagaatcgcg cgctgatcga taatcatacc gaccgtgaga
      601 tggagcatca tcttgagata taaatcagga tcgcccagac cataaatcgc ggaaacagac
      661 gccactacaa tcacatcacg ccgctccagc atcgctttgg tggcggacaa acgcatctgc
      721 tcaatatgtt cgttaaccga ggcatctttc tcaatgaagg tgtcggaact cggtacatag
      781 gcttccggct gatagtagtc gtagtaggag acgaaatact ccaccgcgtt ttccgggaag
      841 aactctttca tttcgccata cagctgggcc gccagcgttt tgttgggcgc aagtaccatg
      901 gttgggcgct gaaggtcagc aatgacattg gcaatggtga aggttttccc ggagccagtc
      961 acgccaagta acgtctggtg cgccaggcca tcttccagcc cctcttcgag acgtcgaatc
     1021 gcctctggct gatcgccaga aggtttaaaa gcggaattca gtttgaacgg tttactcatg
     1081 agtcgctacc tgaaggagtt gggcgggcag gtatgtaatt ttactcgtcg tacttaatta
     1141 tgccaacaaa ttatactgga taaaaaaaca gttcatcacc ataatatttc tgatacagcg
     1201 taaactccgc tgtcaatcat gagcaaaatt tactctgtgg cgagataaaa ctccagcctt
     1261 accgggttat ccccaaagca acggcttttt taacatttgt caagatgagt gatgacagtt
     1321 ttatggcaag agatgcctgt tcagtgactg ccattgcatt tatctaacca gttaaaaaat
     1381 aaaagatatt tctttgagcc gtctttaacg ccaattcgcg tgcaagccgc gtattctctc
     1441 gcttgcctcg tgttttctaa ctctaataca catggttatc cacaggaata gtggataact
     1501 gtctccagcc cctatccccc gccgcctggg caattcccac attcgccgca tgaggcggaa
     1561 gtaaaatttt tttcgtgatt attttagaat ttaattggtt aaattgcagt caatcgaaga
     1621 cgcgatctcg ctcgcaattt aaccaaatac agaatggcta caacaaggca aggtttatgt
     1681 actttccggt tgccgcattt tctggatttt ctgcaagcca ggggatctct cccagcagcg
     1741 gcgcgggaat catgcgggtg agcgtggtca tatattcagc gtgacgtttt cccggaggcg
     1801 taacatcgtt cgccacccaa cccgccagag tcagtccggc gtgttgtatt gcctgcgcag
     1861 tcaacatcgc gtgattaata cagccgagtt tcacaccaac taccagtatc accggcagtt
     1921 gttcctgtgt tacccaatct gcaaaagtga aagtgtcaga aagcggcgta aaccagccgc
     1981 cagcaccttc cactaacacc cagtcagcct gttgttcaag cgcgcgtaat ccggcgctca
     2041 ttaccaatga ttctatcggc ctgccctctt gggcgctgat gatgtgcggc gaggtggatt
     2101 ctgcgaaggt gtaaggattt accgttgcgt aatccagctg caggctgctg ttgtgctgta
     2161 acgccagcgc gtcactatta cgtaagcctt ccggggtctt ttcgctgcca gaggcgaccg
     2221 gtttataacc tgccgtccga taacctactg ccttcgcggc ttgtaaaagt gcacaactgg
     2281 cgacggtttt ccccacttcg gtatccgttc cggtgacaaa ataacgttta ctcacgagca
     2341 atcactccca aaaaaagatg atacgtcaga ggatatcgcc cctgttgttg cggccaggcc
     2401 agttgcaatc gctgcaactg cgaacgcgtt aatattcgcg ggtcgcgccc ttcatgaaga
     2461 tgcgtagcac cgatgccttt cagcgaacgc atggcactga gcgcatcatc aaaccatagt
     2521 gtgatgagct gaatatgatg ttgataacgc acgccgttca gcgactcttc gatttcatct
     2581 ggcggtaaaa agcgattagc atgcggacgc tcgtccaccg cctgccgcgc ctgatgcagt
     2641 tcgggtaacg atccctgcac cagcgtggta aacgcgacca cgcctccggg gcgcaccacc
     2701 cgatacagct cgcggagtgc cgtggataaa ttaccgcacc actgcaccgc gagattgctc
     2761 catgcaagat cgaacgtcgc agtcgctaac ggcagggatt cgatatctcc tgccagataa
     2821 tgatctgcgg catcttta
//
LOCUS       DADLTY010000311         2812 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.315, whole genome shotgun sequence.
ACCESSION   DADLTY010000311 DADLTY010000000
VERSION     DADLTY010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.315"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            65..829
                     /locus_tag="JN334_004255"
     CDS             65..829
                     /locus_tag="JN334_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HAY7440168.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            849..1787
                     /locus_tag="JN334_004256"
     CDS             849..1787
                     /locus_tag="JN334_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAY7440169.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            1843..>2812
                     /locus_tag="JN334_004257"
     CDS             1843..>2812
                     /locus_tag="JN334_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAY7440170.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIAS"
BASE COUNT          669 a          709 c          786 g          648 t
ORIGIN      
        1 ctattttcag gccattttta ccttccatcg gagatggttc cgtatgcgac tcacaggaga
       61 aatcatgaaa ataataacct gctttaagct ggtgcctgaa gaacaggaca ttgttgtcac
      121 tccagaatac accctgaatt tcgacaatgc cgacgccaaa atcagccagt tcgatctcaa
      181 tgccattgaa gctgcaagcc agctcgcaac agatgacgat gagatagccg cgctgaccgt
      241 tggcggctca ttgttgcaga actcgaaagt gcgcaaagac gtgctatccc gcgggccgca
      301 cagcctgtat ttggtgcagg atgcgcaact tgaacatgca ctgcctctcg ataccgcaaa
      361 agcgctggcg gcagcaattg aaaagatcgg cttcgattta ctgatctttg gtgaaggttc
      421 cggcgacctt tatgcccagc aggttggctt gctggtcgga gaaattctgc aacttccggt
      481 gattaatgca gtgagtgcta ttcagcgtca gggcaataca ctggtgattg aacgcacgct
      541 tgaagatgat gttgaagtta ttgaactctc tgttccagcc gtgctctgcg tcacctccga
      601 tattaacgtg ccacgcattc cttcgatgaa agccattctc ggcgcgggta aaaaaccggt
      661 aaatcagtgg caggcaagtg atattgactg gagccagagc gcgccacttg ctgaactggt
      721 cggcattcgc gtaccgccgc aaacagaacg taagcacatc attctcgata acgattcgcc
      781 ggaggccatt gccgagctgg cggaacatct gaagaaagcc ctgaactgaa gcctacggag
      841 aagaaacgat gagtcaatta aacagcgtct gggtctttag cgataatcct gaacgttatg
      901 ctgaactgtt tggcggcgct cagcaatggg gccaacaggt gtatgccatt gtacaaaata
      961 ccgaccaggc gcaggcagtt atgccttatg gtccaaaatg tatttatgtt cttgagcaaa
     1021 acgacgcgct gcaacgcact gaaaattacg ccgaaagcat tgctgccctg ctgaaagata
     1081 aacaccccgc tatgttgctg ttggccgcga cgaaacgtgg taaagctctg gcagcacggt
     1141 taagtgtgca actgaatgcg gcgctggtga acgatgccac ggcggtggat attgtcgatg
     1201 gtcacatttg cgccgaacac cggatgtatg gcgggttagc gttcgctcag gaaaagatca
     1261 acagcccgct ggcgatcatt acccttgcac ccggtgttca ggaaccgtgc accagtgata
     1321 cctctcatca gtgcccgaca gaaacggtac cttatgttgc tccgcgtcat gaaattctct
     1381 gtcgcgaacg ccgtgcgaaa gccgcaagca gcgtggacct gagcaaagca aaacgtgtgg
     1441 ttggcgtcgg tcgtggtctg gcggcgcagg atgacctgaa aatggtccac gaactggcgg
     1501 cggtgctgaa tgctgaagtc ggctgttcac gtccaattgc cgaaggcgag aactggatgg
     1561 agcgtgaacg ttatatcggt gtctccggcg tgttgctgaa atccgatctc tacctgacgc
     1621 tggggatctc cgggcagatc caacatatgg ttggcggcaa cggcgtaaaa gtgattgtcg
     1681 ccatcaataa agataaaaat gcgccaatct tcaactatgc cgactacggt ctggtgggcg
     1741 atatctacaa agtcgtccct gccctgatta gccagttgag ccgctaattc ccctttcaac
     1801 cacatctccc gctgtaaacg cagcaggaag ggagcataac gcatgtcgga tgacaaattt
     1861 gatgccattg tggtcggtgc gggcgttgct ggtagcgttg ccgcactggt catggcacga
     1921 gccgggctgg atgtcctggt gatagaacgc ggcgacagtg ccggatgtaa aaacatgacc
     1981 ggcgggcgtc tttatgccca cacacttgaa gcaatcattc caggctttgc agtatcagcg
     2041 ccggtagaac gcaaggtcac acgcgagaaa atctccttct taaccgaaga aagcgccgtt
     2101 accctcgatt ttcaccgcga gcaaccagat gttcctcaac acgcatctta taccgtattg
     2161 cgtaatcgtc tggacccgtg gttgatggaa caagccgagc aggcgggcgc gcagtttatc
     2221 ccaggcgttc gcgtcgacgc gctggttcgt gaaggaaaca aggtcactgg cgtgcaggct
     2281 ggggatgata ttctcgaagc gaatgtggtg attctggctg atggcgttaa ctcgatgctt
     2341 ggccgctcgc tgggaatggt tcccgcttcc gatccgcatc attacgctgt tggtgttaaa
     2401 gaggttattg gcctcacacc agaacagatc aacgatcgct ttaatattac gggcgaggaa
     2461 ggtgccgcct ggctgtttgc cggttcccct tctgacggcc tgatgggcgg gggatttctc
     2521 tataccaaca aggattccat atccttgggg ctggtttgtg gattgggtga tatcgcccat
     2581 gcgcaaaaaa gcgtgccgca aatgctggaa gattttaaac aacaccccgc cattcgcccg
     2641 ctgattagcg gcggcaaact gcttgaatat tccgcgcata tggtgccgga aggcggtctg
     2701 gcaatggtgc cgcaactggt taacgagggc gtgatgatcg ttggtgacgc cgcaggcttc
     2761 tgcctgaatt tgggttttac ggtccgcggc atggatttag ccattgcatc gg
//
LOCUS       DADLTY010000312         2739 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.316, whole genome shotgun sequence.
ACCESSION   DADLTY010000312 DADLTY010000000
VERSION     DADLTY010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.316"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..142)
                     /locus_tag="JN334_004258"
     CDS             complement(<1..142)
                     /locus_tag="JN334_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HAY7440171.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
     gene            complement(154..1152)
                     /gene="yiaK"
                     /locus_tag="JN334_004259"
     CDS             complement(154..1152)
                     /gene="yiaK"
                     /locus_tag="JN334_004259"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HAY7440172.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            1352..2200
                     /gene="yiaJ"
                     /locus_tag="JN334_004260"
     CDS             1352..2200
                     /gene="yiaJ"
                     /locus_tag="JN334_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HAY7440173.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            2302..>2739
                     /locus_tag="JN334_004261"
     CDS             2302..>2739
                     /locus_tag="JN334_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7440174.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEK"
BASE COUNT          661 a          674 c          737 g          667 t
ORIGIN      
        1 gcgcataaat attcttgccg tcgatttcga caacgcccgg ctccaggacg ttgaagttgg
       61 tagcgcgcag aaaatcgagc gccttttcaa tggcggcggg caaacggcac ggattgggct
      121 gcgcgatatg tccaaaaatc atgacttatc tcctcataac gcctggattt tggcccacac
      181 gctgtcatca acagtgatgc cgttacggcg gttttcggcc agcagggtag taaattcgtg
      241 gccgggtaag cggatggcct ggttttcgtc agcgcgttca gcggtagtaa cgtaatccat
      301 gatacgttgc agcttggcat cgcgggtggg accgtcgata agcttgtcca cttcaatggc
      361 aataaaaatt tgtgaaatgc cgtattcgtc gctgttgtcc tgggtgactt cggcaaccga
      421 tgcgccgtcg gaaaggagag tagcgatcat atccagcaca atcgacatgc cagaaccttt
      481 ccagtagccc atcggcaaaa tgcggcgatt cttctcgata acgccaggtt ctttggtcaa
      541 attgccctca tcatcaaagc caccatcgac cgggagctga cggcctgcca ggcggttaac
      601 ttctaacatg ccgtaagaga acatcgacat cgacatatcg accatggtga tcggcgtgga
      661 aggaatggcg acgatcagcg ggttagtgcc gatgcgacac tcttttgcgc cccacggcgg
      721 cattacggcg atggagttgg tccagcaaat gccaatatag cctttttccg ccgcctgcca
      781 gccgtagctg ccgccgcgca tccagtggtt ggcattacga agtgccacca gaccaatacc
      841 gtgatcggca gccagttcaa tggcgcgatc catcatcttt ttcgccgtca ggttgccgat
      901 cgaacgctgg gcatcccact gttcaattgc gccgaggctg gttatacgtt tgggttgggc
      961 atcaggaatg atatcgccgt tttccagttg ttgaatgaaa cgagggaaac gattaacgcc
     1021 gtgagaataa acgccggatt cggtggtgcg ggcgaacatc tctgcacagg cgtcagccgt
     1081 ttcgctgtca acgccgcgtg aaattaagac ccgattaaag gctgctttta actgctcaaa
     1141 tgtcactttc atcctaggct tccttgtttt ttgatcgctt ttttggctta aaatttcaat
     1201 atgcggaact agatttcaaa tatatcgatc actttttaac aaagcaatct gattgatgaa
     1261 tttcaaaaga cataaaaatc aagtggttat aaattatctg tcgagatcgt gaactacggc
     1321 acactttgcg ctaccatcag gacgcgacaa aatggggaaa gaagtgatgg gaaaaaaaga
     1381 gaacgagatg gcgcaggaaa aagagcgtcc agccggaagc cagagtctgt ttcgcgggtt
     1441 gatgctgatt gagattttga gcaactatcc aaacggttgt ccgttggcgc atctttcgga
     1501 gctggctggt ttaaataaga gtaccgtcca tcgcttattg cagggattac agtcctgcgg
     1561 ctatgtgacc accgcgcccg ccgcagggag ttaccgcctg actaccaaat ttattgccgt
     1621 cgggcagaag gcgctgtctt cgctgaatat cattcatatc gccgctccgc atcttgaggc
     1681 actgaacatc gccactggtg aaaccattaa cttctccagc cgcgaagacg atcacgctat
     1741 tttgatttat aagctggaac ccacaaccgg gatgctgcga acccgtgcct atattggcca
     1801 gcatatgccg ctctactgtt ccgcaatggg caagatctac atggcgtttg gtcacccgga
     1861 ctacgtgaag tcatactggg aaaaccatca gcatgagatc cagccgttaa cccgcaatac
     1921 cattaccgag ctgcccgcga tgttcgacga actggcgcac attcgtgaaa gcggagcggc
     1981 gatggacaga gaagaaaacg aactcggcgt ctcctgtatt gctgttccgg tgtttgatat
     2041 tcatgggcgg gtgccgtacg ccgtgtcgat ttcgctttcg acatcacgtc tgaaacaggt
     2101 gggagagaaa aatctcctga aaccactgcg tgaaaccgcg caggctattt ctaatgaact
     2161 gggatttact gtcagggatg acctgggcgc gatcacataa cgctttggac aagtgccaaa
     2221 actttaacat ttccttcgtt ggatcaaagc agtagggacg cgctctctgg cactctgctg
     2281 ttttagtgca aaggaatgat catgaaccgg tttattattg cggatgcgac gaaatgtatc
     2341 ggttgccgta cctgtgaagt ggcttgcgca gtgtcgcatc aggagaatca ggattgcgct
     2401 gcgttgtcac cagacgagtt tatttcccgt attcgtgtca ttaaagacca cagctggacc
     2461 acgccagtag cctgtcatca gtgtgaagat gcaccgtgcg cgaatgtctg ccctgttgac
     2521 gcgataagcc gcgaacatgg gcatattttc gttgaacaaa cacgttgcat tggctgtaaa
     2581 agctgtatgc tggcttgccc gtttggtgcg atggaggtcg tttcttcgcg caaaaaggcg
     2641 agggcgatta agtgcgatct gtgctggcat cgggagacgg ggccggcctg tgttgaagcc
     2701 tgcccgacaa aggcgttgca gtgcatggat gtcgagaaa
//
LOCUS       DADLTY010000313         2713 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.317, whole genome shotgun sequence.
ACCESSION   DADLTY010000313 DADLTY010000000
VERSION     DADLTY010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.317"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            52..2574
                     /locus_tag="JN334_004262"
     CDS             52..2574
                     /locus_tag="JN334_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7440175.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2585..>2713
                     /locus_tag="JN334_004263"
     CDS             2585..>2713
                     /locus_tag="JN334_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7440176.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          727 a          585 c          678 g          723 t
ORIGIN      
        1 agtgacccga taaaaatgac gctgtaagca agggataagg ataggcgtat tatgatgacg
       61 accagaatag tggttggcct cacggcaggg acgtgtctga ttttctcgca aaacctgatg
      121 gccgaggtca gtgtattcaa tccggcgctt ctggaaatca accatcaatc cggagtcgat
      181 attcgccagt ttaatcgggc aaacctgatg cccccaggtg tttatagcgt tgatattttt
      241 atcaacggta aaatgtttga acgtcaggat gtgacatttg ttcaggataa tccagatgct
      301 gatctgcacg cttgctttat tgccattaaa aaaacactgt cctcctttgg cataaaagtt
      361 gatgcgctca aatcgttcaa tgatgtggat gagacggttt gcctcgatcc tgctccacgt
      421 attgaaggct catcctggca gtttgacagt gataaattgc agctgaatat atccattcac
      481 caaatctaca tggacgcgat ggcttatgat tacatcagcc ccacgcgttg ggatgagggg
      541 attaatgcgc tcaccatcaa ctacgatttt tctggttcac atacactacg ttcagattat
      601 ggttcacaag agacagatac cagttatctc aatctgcgca atggactgaa tattggaccg
      661 tggcggctac gtaattacag tactttaaac accagcgatg gccgtgcgga atacaactcc
      721 attagtacct ggatacagcg cgatattgcc gcgttaagaa gccagattat gattggtgat
      781 acgtggacgg cgagcgatat tttcgacagt acgcaaattc gcggcgcgcg tttgtatact
      841 gataacgata tgctacccgc cagccagaat ggctttgctc ctgtggttcg tgggattgca
      901 aagtccaacg ccaccgtcat cattcggcag aatggctacg tgatttatca gtcagccgtt
      961 ccacaaggtg cttttgagat caccgatctc aacaccgcaa gtacaggtgg cgatttggac
     1021 gtaaccatca aagaagaaga cggtagcgaa caacgattca cccaacctta tgcttcattg
     1081 gcgattctta aacgtgaagg tctgacagat gttgatgtca gcgtgggtga attgcgcgat
     1141 gaagacggat ttacaccgga cgtccttcag gcgcaaatac ttcatggttt ttcccacggg
     1201 atcactttat atggaggtat gcaggctgct gaaaattatg gttctgcagc tctgggtgtc
     1261 ggtaaagatc ttggcgcttt gggcgcaatt tctttcgatg tgacacatgc tcgtgcgaat
     1321 tttagccatg atgatacaga aacgggtcag tcatatcgct ttctctattc aaaactattt
     1381 gacgacacag acactagctt gcgcctggtt ggctatcgtt actccaccga gggctactat
     1441 accctcaatg agtgggcatc gcggcgcaac agccctgaag acttttggga aacaggtaac
     1501 cgacgtagtc gcgtggaggg aacgctaacg cagtcgttgg ggagagatta tggcaattta
     1561 tacctgacat taagccggca acaatactgg cataccgatg atgtcgaacg attaatgcaa
     1621 tttggctaca gcagtagctg gaagcgtctc tcgtggaacg tctcctggag ttattccaat
     1681 actgccagac aggggacggg gaacaaccat gccagtgata acaccagtga gcagatctac
     1741 atgctctctt tatctgttcc tttatcgggc tggtggggta atagttacgc cacctattct
     1801 gtttcgcaaa acgataattc cggtagctca catcaactcg gactcagcgg tacggcgctg
     1861 gaaagaaata acctttcatg gaatttaatg cagtcctata acagtcatga tgatgaggtt
     1921 ggcggtaata tgtccctgac ctatgatggc tcttatggca cggtgaacgg cagctataac
     1981 tacagccaaa attcccagag gctgaattat ggtatcagag ggggaattct ggcacacagc
     2041 gaaggggtaa cgttaagtca ggagttaggt gaaactattg ctcttgttaa agcacctggg
     2101 gccgccaggt tagaaataga taatatgcgc ggtgctgcga cggactggcg cggctatacg
     2161 gtcaagacac agctaaaccc ttatgatgaa aatcgggtag caatcagcga taactatttc
     2221 tcgaagtcga atatagaact tgataatacc gtcgttacga tggttcccac gcgtggtgca
     2281 gtggttaaag cggagtttgt gactcatgtg ggttatcgcg ttctcttcag agtgttaaat
     2341 gcaaatggta aaccggtacc ttttggagcc attgctgcga tacaagatgc aagtttggca
     2401 gattcaggaa ttgtcggtga ccgtggcgaa ctttatcttt ctggtctacc agaaaaagga
     2461 caggttacgt tatcctgggg agaaaacgcc tcaacaaaat gcatcttcaa ttattcattt
     2521 tcgacaccag aaagtgagag cggattaatt gaacagggtg tgacatgtca ttaaaaggat
     2581 ttttatgaac aagtatatca aacagtggtg ctttgctgtg tttatgctct cgttaagtag
     2641 tgtagcactt gcagctccta aaggtatctg tacccctgat aatggcgtat ttcacagtac
     2701 gcttgatttc tcg
//
LOCUS       DADLTY010000314         2697 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.318, whole genome shotgun sequence.
ACCESSION   DADLTY010000314 DADLTY010000000
VERSION     DADLTY010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.318"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            258..1673
                     /gene="gltX"
                     /locus_tag="JN334_004264"
     CDS             258..1673
                     /gene="gltX"
                     /locus_tag="JN334_004264"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAY7440177.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(1725..2096)
                     /locus_tag="JN334_004265"
     CDS             complement(1725..2096)
                     /locus_tag="JN334_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY7440178.1"
                     /translation="MTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGGRARLILV
                     DTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTDIEQEKVA
                     QFLSREGIRNFLARLDIDESA"
     gene            complement(2119..2463)
                     /locus_tag="JN334_004266"
     CDS             complement(2119..2463)
                     /locus_tag="JN334_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY7440179.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
BASE COUNT          666 a          649 c          674 g          708 t
ORIGIN      
        1 cgctgtgtgg agtcgcatta tagggagagt tcaaaatgag tcaacgcatt ttctaaagaa
       61 attgttcgtt cgtcgtaaat ttaagcaaga tgatcgcaaa acagaccgtg ttgcgcaatt
      121 tgtcaacgaa aacaataatg cgtaaggtag aaacccgaac tacattgagg aatcaggcgg
      181 gagtgataga atatcgccca ctcaattttt ccaggatttg ccggttgtcg gcatctttct
      241 aaacgtaagg ccatttcatg aaaatcaaaa ctcgcttcgc gccaagccca acaggctatc
      301 tgcacgttgg cggcgcgcgt actgctcttt actcctggct ttttgcacgt aaccacggcg
      361 gtgagttcgt gctgcgtatt gaagacaccg atcttgagcg ttccacgccg gaagctatcg
      421 aagccattat ggatggcatg aactggctga gcctggagtg ggatgaaggt ccgtactacc
      481 agaccaaacg ttttgatcgc tacaacgcgg tgatcgatca gatgctggaa gagggcactg
      541 cttataaatg ctattgctct aaagagcgcc tggaagcgct gcgcgaagag caaatggcga
      601 aaggtgagaa gccgcgttat gacggtcgct gccgccacag ccatgagcat catgctgatg
      661 atgaaccgtg tgttgtacgt tttgctaacc cgcaggaagg ttctgttgtt tttgacgatc
      721 agatccgtgg tccgatcgag ttcagcaacc aggaactgga cgatcttatt atccgccgta
      781 ccgatggttc cccaacctat aacttctgtg tggttgtcga tgactgggat atggaaatca
      841 cccacgttat ccgtggcgaa gaccatatca acaacacgcc gcgccagatc aacattctta
      901 aggccctgaa agcgccggtg ccggtttacg cgcacgtttc tatgatcaat ggcgatgacg
      961 gtaaaaaact gtccaaacgt cacggggcag tcagcgtaat gcagtatcgt gatgacggtt
     1021 atttgccaga agcactgctg aactatctgg tgcgtctggg ctggtcccac ggcgatcagg
     1081 aaatcttcac tcgtgaagag atgatcaaat acttcacttt gaatgccgtc agcaaatctg
     1141 ccagtgcgtt caacaccgac aagctgctgt ggctgaacca tcactacatt aacgcgctgc
     1201 cgccggagta tgttgctact cacttacagt ggcacattga gcaggaaaat atcgataccc
     1261 gtaacggccc gcagctggct gatctggtga aactgctggg cgaacgctgc aagacgctga
     1321 aagagatggc acagagctgc cgttatttct acgaagattt tgctgagttc gatgccgatg
     1381 cggcgaaaaa acatctgcgt ccggtagcgc gtcagccgct ggaagtggtt cgtgacaaac
     1441 tggccgcgat tactgactgg accgctgaaa acgttcatca cgctattcag gcgacggcgg
     1501 atgagctgga agtgggtatg ggtaaagttg gtatgccgct gcgtgtcgcc gtaaccggtg
     1561 cggggcagtc tccagcactg gatgttaccg ttcacgcaat tggtaagacc cgcagtatcg
     1621 agcgtatcaa caaagcgctg gattttattg ctgaacgcga aaatcagcag taattagcgt
     1681 gtaaaaagat aaacggcagg agataatatc ctgccgtttt ttatttatgc tgattcgtca
     1741 atatcgagac gggcgaggaa attgcgaatt ccttcacgtg acaaaaattg cgcgactttt
     1801 tcctgctcaa tgtctgtcat attatctatc gcgttgatga tttgtcgata cgccggggcg
     1861 cgtgctccag gagcgtattc agcaattctc tcttcagctt ccatcaacat tggtgtgtta
     1921 tggaaggctg gcgtgttctg aataaactcg caaacttgcg tatcgaccag aataagtctg
     1981 gcacgaccgc ctttcacgcc gggaaatttt tccgttttcc agcctttttc tctggtccaa
     2041 cggttaacag tttgtttagc cacaccgagg cattcagcca gttcttcggt ggtcatttta
     2101 ttgcgtaatc ttttcatatt atttgctatc gcgaatccct aagcgttgta acaatccgat
     2161 aatcccttcc cgcagaagca aggatgtaaa ctgtttttgt tcaactggcg tcatctcttt
     2221 cgccagtgtc accagcaata cttcaagcgg cgcatcacca gaaagggcag gcgcttctcc
     2281 ctgaccttct ggacgttcag cattgcgaat atattcacga acctgttcat tgacgtgaac
     2341 cagtcgggct ttgccaccct ggacgccagg ttttggtgac gttgtccagc cttccttgcg
     2401 tacccattta ttaatggtct ggcggctata gccggtcagt ctggcaagtt catctggtgt
     2461 catcctctcc ttgaacataa caattccctg aatgtatgtg ggtctattag tagttcattt
     2521 tataacacca ctttacagga aaccgtgaca tgaataacta ctgaatgcga gcggttttcc
     2581 aaaattgttc atttgcctga tttttcagcg attgaaacta cacgttgcaa tttgccgttg
     2641 acacattcgg gcggaattca tatgatgccg cccgtcaact cgacaagctt tacgtga
//
LOCUS       DADLTY010000315         2694 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.319, whole genome shotgun sequence.
ACCESSION   DADLTY010000315 DADLTY010000000
VERSION     DADLTY010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.319"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..120
                     /locus_tag="JN334_004267"
     CDS             <1..120
                     /locus_tag="JN334_004267"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HAY7440180.1"
                     /translation="ERELEMSTEIARLKRQLAERDEELAILQKAATYFAKRLK"
     gene            117..983
                     /locus_tag="JN334_004268"
     CDS             117..983
                     /locus_tag="JN334_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7440181.1"
                     /translation="MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQ
                     FRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKF
                     SPVSYRAHGLPVSENLLEQDFYASGPNQKWPGDITYLRTDEGWLYLAVVIDLWSRAVI
                     GWSMSPRMTAQLPCDALQMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSM
                     SAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGG
                     LSPEQFENQNLA"
     gene            complement(1087..1554)
                     /locus_tag="JN334_004269"
     CDS             complement(1087..1554)
                     /locus_tag="JN334_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAY7440182.1"
                     /translation="MYGTWSLSMAAIWLLRVELLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(1620..1859)
                     /locus_tag="JN334_004270"
     CDS             complement(1620..1859)
                     /locus_tag="JN334_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HAY7440183.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1994..2599)
                     /locus_tag="JN334_004271"
     CDS             complement(1994..2599)
                     /locus_tag="JN334_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7440184.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          783 a          628 c          593 g          690 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt
      421 tcagcccggt cagctaccgc gcacacggcc tgcctgtgtc agaaaatctg ttggagcagg
      481 atttttacgc cagtggcccg aaccagaagt ggccaggaga catcacgtac ttacgtacag
      541 atgaaggctg gctgtatctg gcagtggtca ttgacctgtg gtcacgtgcc gttattggct
      601 ggtcaatgtc gccacgcatg acggcgcaac tgccctgcga tgccctgcag atggcgctgt
      661 ggcggcgtaa gaggccccgg aacgttatcg ttcacacgga ccgtggaggc cagtactgtt
      721 cagcagatta tcaggcgcaa ctgaaacggc ataatctgcg tggaagtatg agcgcaaaag
      781 gttgctgcta cgataatgcc tgcgtggaaa gcttctttca ttcgctgaaa gtggaatgta
      841 tccatggaga acactttatc agccgggaaa taatgcgggc aacggtgttt aattatatcg
      901 aatgtgatta caatcggtgg cggcggcaca gttggtgtgg cggcctcagt ccggaacaat
      961 ttgaaaacca gaaccttgct taggcctgtg tccatattac gtgggtagga tcacaagacc
     1021 tactgctatc acaattactg agagaaaaaa aagcatccga tgattcattt atgctgtcct
     1081 aaaaaattaa caaaacaatg ggaaaacagg atatgtaagc agaaaagcca atgaaattgg
     1141 tacagcataa ggcacaccgt tttcacgaat tgattttcga aaaaaaagaa gacctgccag
     1201 aacaaccaga gcgccgcaaa aggtcattgc gacgagaaaa ttagccagtt catgttcatc
     1261 aatagctaac gaaagaacag ttaacagctt gacatcaccg ccgccaatta tccctgcgga
     1321 aaaaagaaag aagcccacga gcaaaacgct cccggctgca accagattgg gtttttgatg
     1381 ctgtaaaatg agtgccaaaa aaataagcac ggttaacacc agtaaataac gatggctaat
     1441 aattctgagt aatatatccc cccatgaaat aaaaataaga aagagcgcaa caaataaaag
     1501 ttcgactctt agtaaccata ttgctgccat tgaaagactc catgtaccat acaaggttgc
     1561 tgccagacat attcctttac gtctgacagc ctctagagag tttttttatt ttttataagt
     1621 tacggattac tcgttccgga aatcgttacc tgttgaattg ctgcagcgat agcgtcgaat
     1681 gcatctttaa ttgcgcccag gaagcctgag ttctgatcgc caaagatgaa agcaagcaga
     1741 gttgccatag caacaccaat caatgcatat tcgattgcag taacaccacg ttcatccttc
     1801 gcgaaacgcg caaagcactg tttagtttta ccatagtact tagtaataaa agtattcatg
     1861 ttttcctcgt atattattat ttaaattcaa accaccagct tcatctgcgt gtcaccggta
     1921 tcccatattt ttgggatgtg gctataatta tctattcccg ctatttagtc ttcaaaaaaa
     1981 attacattta atatcattta tcttgattaa gaaatagtaa gactaaaaaa acaagcgtcg
     2041 tcagccagca cactgctaac acggtaagaa cgctaataaa aacactccac tgcttcgttc
     2101 tataccattc aatatttttg tcagtatgtt ttattgggag cagctcttca gtagagaatt
     2161 cctggctgtt atcattctca ggaatgtttg catcagtgtt tacttccctg gcctcgctat
     2221 cgttggtata gctactactg tcttcatcac tgtcacgctc attctcacag acatgtttct
     2281 cgacctttcc cagatatctg acgccttttc gcggaatggt tttaattaga gaactcgaca
     2341 aacctgcgac gtctaatgcc tttctaagca tgtaaatctg cccataataa ctgctgtcgc
     2401 ttaccgaacc tttctgctcc cgccaaacct cattgataat atgttgctta tcggattctc
     2461 cctgtagtaa aaggagtaaa aatcgatagc cgttttcaga cagattgatt acattgcctg
     2521 tactggtggt gatacgtcga gacgctgggt aaaacttaac tccctgccct aaagaaaaat
     2581 aaaaacggtc atcgctcata agtaccgacc cctgttttct tcttcacatg actactcatt
     2641 acaactttgt atgaagtaag atgcaaaatc atattgttta cattaagtta agca
//
LOCUS       DADLTY010000316         2679 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.320, whole genome shotgun sequence.
ACCESSION   DADLTY010000316 DADLTY010000000
VERSION     DADLTY010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.320"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            168..1271
                     /gene="proB"
                     /locus_tag="JN334_004272"
     CDS             168..1271
                     /gene="proB"
                     /locus_tag="JN334_004272"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY7440185.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1283..2536
                     /gene="proA"
                     /locus_tag="JN334_004273"
     CDS             1283..2536
                     /gene="proA"
                     /locus_tag="JN334_004273"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY7440186.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT          637 a          678 c          768 g          596 t
ORIGIN      
        1 tggtaattta ttaaatctgt aataaaagcg taaacaactg ccgctacgct tgctgatccc
       61 gcgcaacaaa acgccatgct ttgctcgcag atggttggca accgacgaca gtcctgctaa
      121 aacgttcgtt tgatatcatt tttcctaaaa ttgaatggca gagaatcatg agtgacagcc
      181 agacgctggt ggtaaaactc ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc
      241 gtgcccatat cgttgaactt gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga
      301 ttgttattgt gacgtcgggc gcgatcgccg ccggacgtga gcacctgggt tacccggaac
      361 tgccagcgac tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc
      421 aactgtggga acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc
      481 gtgctgatat ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc
      541 tcgataacaa tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta
      601 aggtcggcga taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac
      661 tgttgctgct gaccgatcaa aaaggtttgt ataccgctga tccgcgcaga aatccgcagg
      721 cagaactgat taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca
      781 gcgtttcagg cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt
      841 gccgtgcggg tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg
      901 tgatggaagg catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc
      961 gtaaacgctg gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa
     1021 ctgccgccat tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg
     1081 gcaatttctc gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc
     1141 acggtgtcag tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag
     1201 aaattgatgc aatactggga tatgaatacg gcccggttgc cgttcaccgt gatgacatga
     1261 ttacccgtta aggagctggc cgatgctgga acaaatgggc attgccgcga agcaagcctc
     1321 gtataaatta gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga
     1381 tgaactggaa gcacaaagcg aaatcatcct caacgctaac gcccaggatg ttgctgacgc
     1441 gcgagccaat ggccttagcg aagcgatgct tgaccgtctg gcactgacgc ccgcacggct
     1501 gaaaggcatt gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt
     1561 aatcgatggc ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg
     1621 ggttattggc gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg
     1681 cctgaaaacc ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc
     1741 ggcaacggtg gcggtgattc aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt
     1801 gcaggcgatt gataatcctg accgtgcgct ggtcagtgaa atgctgcgta tggataaata
     1861 catcgacatg ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc
     1921 gacaatcccg gtgatcacag gtggtatagg cgtatgccat atttacgttg atgaaagtgc
     1981 agagatcgct gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg
     2041 taatacggtt gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt
     2101 aagcaaacaa atggcggaaa gcggcgtgac attacacgca gatgcagctg cgctgacaca
     2161 gttgcaggca ggccctgcga aggtggtggc ggttaaagcc gaagagtatg acgatgagtt
     2221 tctgtcatta gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat
     2281 tcgtgaacac ggcacacaac actccgatgc gatcctgacc cgcgatatgc gcaacgccca
     2341 gcgttttgtt aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta cgcgttttac
     2401 cgacggcggc cagtttggtc tgggtgcgga agtggcggta agcacacaaa aactccacgc
     2461 gcgtggccca atggggctgg aagcactgac cacttacaag tggatcggca ttggtgatta
     2521 caccattcgt gcgtaaataa aaccgggtga tgcaaaagta gccatttgat tcacaaggcc
     2581 attgacgcat cgcccggtta gttttaacct tgtccaccgt gattcacgtt cgtgaacatg
     2641 tcctttcagg gccgatatag ctcagttggt agagcagcg
//
LOCUS       DADLTY010000317         2648 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.321, whole genome shotgun sequence.
ACCESSION   DADLTY010000317 DADLTY010000000
VERSION     DADLTY010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.321"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..246
                     /locus_tag="JN334_004274"
     CDS             <1..246
                     /locus_tag="JN334_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440187.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="JN334_004275"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="JN334_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HAY7440188.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="JN334_004276"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="JN334_004276"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY7440189.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..>2648
                     /gene="mdfA"
                     /locus_tag="JN334_004277"
     CDS             1990..>2648
                     /gene="mdfA"
                     /locus_tag="JN334_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HAY7440190.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GR"
BASE COUNT          673 a          646 c          686 g          643 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtagga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggcc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 agcttctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccatg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
     2641 aacggccg
//
LOCUS       DADLTY010000318         2576 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.322, whole genome shotgun sequence.
ACCESSION   DADLTY010000318 DADLTY010000000
VERSION     DADLTY010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.322"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(27..668)
                     /locus_tag="JN334_004278"
                     /pseudo
     CDS             complement(27..668)
                     /locus_tag="JN334_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(750..2138)
                     /locus_tag="JN334_004279"
     CDS             complement(750..2138)
                     /locus_tag="JN334_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HAY7440191.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
BASE COUNT          745 a          610 c          637 g          584 t
ORIGIN      
        1 tcgcaatacc agtcgtagaa actttaacag gtgccgccaa gcagcgacag ataacgcgtt
       61 ccggcggcgt aagaaaccat cgacatcgcc gggatcggcg agaaaccggc aacgcgatcc
      121 gggccgtagg ttttgatggt ccagacgtta gcggcggcaa tcagctggtt tagttcctgc
      181 cagttggagc ggataaaccc gccgcgccca cgcacttgtt tgtagctcag gcacttttgc
      241 ggatcattca taatcgatgc ccacgccagt accggatcgc tgtgttgttt gagggcttcg
      301 cgccacagtt caatcagtcg tttacgaatg agcgggtatt tcaggcggtt agcgctgtaa
      361 agataccagg agtaacttgc gccacgcggg cagccgcgag gttcatgatt gggcaggtca
      421 gggcgagtgc gcgggtagtc ggtctgttgg atttcccagg tcaccagacc atttttaacg
      481 tagattttcc agctacagga gcctgtacag ttaacgccgt gggtggaacg cacgattttg
      541 tcgaactgcc aacgctggcg atagctgtcc tcccagtcgc ggttgctatg catcacctgt
      601 ccgtgaccat cggcaaaggt ttcgcccttt tgtttgaagt agcgaaagcg atccaaaagt
      661 ttactcatga catgactcct gctccaggaa tgaattggga tctgatgcct tgtcggatgc
      721 gacatggttg tacatcaggc atcgatctct tatttttggc tgaacttccg ccgaccataa
      781 accagccagg tcagcagcac acaaacgatg tagaagatta aaaacacttt catcgcgccg
      841 accggagagc cggtcatatt gagcgacatg ccaaatgcct gcggaataaa aaagccgccc
      901 actgcgccaa tggctgagat aaagcccagc gttgccgccg tttcggtgac ggcttctctc
      961 tgagcttgct catcactacc gcctttcatc ttcacccgat aaatggttat ctggcgaaag
     1021 atgacggcga tcatctggaa agtagaacca cttcccagac ccgcggtcag aaacagcccc
     1081 ataaatacgg cgtaaaaggc gatgaaatta ccggagcctg tgcccggtaa ggtaaggaac
     1141 agcagggcac tgaaaatcgc cataaaaata aagttgatca acgtcacccg cacgccgccg
     1201 aatttatcgg aaatagcacc accaaccgac cgcgcgatgg caccaataaa tgggccaaag
     1261 aacgccaggc gcagaatatt cacatccggg aactgggttt ttgccagcat ggcaaaaccc
     1321 gcagaaaaac cgataaacga accgaaggtg gcaaggtaaa gcaggctcag cagccagaga
     1381 tgcaggcgtt gtaagacagg gagctggtcg gcaattgagg cgcgtgaact ggcgatatca
     1441 ttcatccctg accaggcggc gatcgtggca atcgccagta gcggtaccca aatccatgcg
     1501 gcattcgcca gcgacatcac cgaaccgtcg gcctgcggta cgccattgac gccgagaaag
     1561 gcaaatacag gtacaaaaat gaccagcggt gcaaccagct gcattacact tacacctaag
     1621 tttcctaatc cgccattaat cccaagagcg ctcccttgtt tggcttttgg aaagaagaaa
     1681 ctgatattgc ccatgctcga agcaaagttt gcacctgcaa aaccgcatag caaagcgata
     1741 acgataaata tcccaaaagg agtattcgga ttttgcacgg caattccgag ccagacgcaa
     1801 ggaataatca ggattgcagt actaaaaacc ggccatcggc gtccaccgaa tataggcacc
     1861 ataaaggagt agggaacacg caataatgcg ccagaaacgg agggtaatgc ggttaataaa
     1921 aagagttgat cggtagtaaa attaaaaccg attttattga gattaacggt aactgcgcta
     1981 aatagcatcc agacacagaa ggcaagaagt agacaactga ctgatatcca gaggtttctt
     2041 cgagcaatat gttttccttt attttcccag aaggccggat tttctggttt ccagtcgcgt
     2101 aaaagataac gactattttt ctcattttgc agtgccatat tgttcctcat atgcacacat
     2161 tggtaatgaa aaaagacaaa acaggaggta aggcgcaata gccagttatt agaattaagg
     2221 atgaattggg tgaagtgctg attgaaagaa tagataagaa agcgtaaccg cgggggcaga
     2281 atggggatta agtagccaga tatgtgttac taaatgtaac taacaagcta accttcaaac
     2341 gggggttaat ctttgaacgt atgtcatgtt tcaacccttc agatcgtgaa tctaaagggt
     2401 tacatattaa ctatattctc aacaagacca gacacttata gaattagtga tgatttattc
     2461 actaaaaagg ccatgaattg gaggaaaacg atctgtccag aaagtctatc ggttcctctc
     2521 ttattgcctg attgtaataa tcgttattat agtaagtatt gtttatttca tctaag
//
LOCUS       DADLTY010000319         2572 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.323, whole genome shotgun sequence.
ACCESSION   DADLTY010000319 DADLTY010000000
VERSION     DADLTY010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.323"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(64..822)
                     /locus_tag="JN334_004280"
     CDS             complement(64..822)
                     /locus_tag="JN334_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAY7440192.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="JN334_004281"
     CDS             complement(830..1933)
                     /locus_tag="JN334_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY7440193.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..2554)
                     /locus_tag="JN334_004282"
     CDS             complement(1943..2554)
                     /locus_tag="JN334_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY7440194.1"
                     /translation="MAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLPLLAQWL
                     FGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQAVPYGQH
                     EAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDMVSLFAG
                     TVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          711 a          683 c          601 g          577 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat aacggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aa
//
LOCUS       DADLTY010000320         2530 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.324, whole genome shotgun sequence.
ACCESSION   DADLTY010000320 DADLTY010000000
VERSION     DADLTY010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.324"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..413)
                     /locus_tag="JN334_004283"
     CDS             complement(<1..413)
                     /locus_tag="JN334_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAY7440195.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPR"
     gene            625..1389
                     /gene="puuD"
                     /locus_tag="JN334_004284"
     CDS             625..1389
                     /gene="puuD"
                     /locus_tag="JN334_004284"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HAY7440196.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            1416..1973
                     /gene="puuR"
                     /locus_tag="JN334_004285"
     CDS             1416..1973
                     /gene="puuR"
                     /locus_tag="JN334_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HAY7440197.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            2123..>2530
                     /locus_tag="JN334_004286"
     CDS             2123..>2530
                     /locus_tag="JN334_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY7440198.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAID"
BASE COUNT          668 a          625 c          642 g          595 t
ORIGIN      
        1 ttccgcggct caacgtcaaa gggagcgcca tcttcatcga ccatggtcag gagcatctga
       61 ccgataaact ctggatcggc ggcagaagga gttaaggaac cgagaacagg aacacaggta
      121 cgatccggct cgcccatttc ctgacccaga cctgcttctt ctactacgtt gcccagaata
      181 tccattgcaa ataccgaggc cgggaaataa caccctttct cgagcttctt caggcttgaa
      241 acagggatac gttttccacg gaagcaacca ttcaaatcag taagtaaaac atcaacatat
      301 tgcgtattcg ggtagcgctc caggtagcgt ttcacttcct gcgtaaaggc gctaccccgc
      361 ctctcttctg actgctgaac aaagttctct acttcaacga tattggtttc catgattctt
      421 cgcctttggt ttgttttccg ctcgttatca aagcgtaaaa tataatgacc atcattcgaa
      481 tctgtatgca aaccaaatgt ttgtcaaatg ttaaattgag tttgcaaaaa tgaaaatcca
      541 ctgctagatt gaaaaaatat tgaacataaa ggtcatttaa agcgcagtaa tggcgataat
      601 ttagtccatt ttgtgagatt gagcatggaa aatataatga acaatccggt tatcggtgtc
      661 gtaatgtgca ggaacaggct taagggtcat gcgacccaga ctctgcaaga aaagtacctg
      721 aatgccatca tccacgcagg cggcttgcca attgcgctac cacatgcgct ggcggaaccg
      781 tcgttacttg aacaactttt gccgaaactc gatggcattt atcttcctag tagtcccagc
      841 aatgtgcagc cgcacctata tggtgaaaac ggcgatgagc ctgacgccga tcccgggcgt
      901 gatcttctga gcatggcgat aattaatgcc gcactcgaaa ggcgcatccc cattttcgcc
      961 atctgccggg gtttacaaga actggtggtg gcaaccggtg gttcgttgca tcgcaagctg
     1021 tgcgaacagc ctgaattgct ggaacatcgg gaagatcccg aactgccggt ggaacagcaa
     1081 tatgcaccgt cgcatgaagt tcaggttgaa gaggggggat tactgtctgc gttgttacct
     1141 gaatgtagca acttttgggt aaactctcta catggacaag gggcgaaggt cgttagccca
     1201 cggttgcgtg ttgaagctcg ctcgccggat ggcttagttg aggcggttag cgtcatcaat
     1261 catccttttg cgctgggcgt acagtggcac ccggaatgga acagtagcga gtacgcgctt
     1321 tcgcgtatat tgttcgaggg ctttatcacc gcttggcagc accatatcgc tgaaaaacag
     1381 cgactctgac cactacagtt taaggaaatg caaatatgag tgatgaggga ctggcgccag
     1441 gaaaacgctt gtcggaaatc cgccagcagc aggggctttc acaacgtcgt gccgccgaac
     1501 tctccgggct gactcacagt gctatcagta cgatagaaca agataaagtc agccctgcca
     1561 tcagtacgct gcaaaagctg ctgaaggtgt atggtctgtc actctcggaa ttcttttccg
     1621 agccggaaaa acctgatgag ccgcaggtcg tcattaatca ggacgactta attgagatgg
     1681 gcagtcaggg tgtgtcaatg aagctggttc ataacggtaa cccgaatcgc acgctggcga
     1741 tgatctttga aacgtaccag ccgggcacaa ccactgggga aagaattaag catcagggtg
     1801 aggaaatagg cacagtactg gaaggtgaaa ttgttctgac gattaatggt caggattacc
     1861 acctcgtcgc ggggcaaagt tatgccatta ataccggcat cccgcacagt ttcagtaata
     1921 cgtcggcagg tatttgccga attatcagcg cccatacgcc caccacgttt taatcttttt
     1981 gttctgtaag ccgggtaagc gcaatgcgcc cggcaatcca taaagaatgt cgccagaaat
     2041 aaacgggcat gcagcccggg gatctctgcg ccctgacgtt cacaaactgc atatatctga
     2101 tagacgtgaa acaggagtca taatgaattt tcatcatctg gcttactggc aggataaagc
     2161 gttaagtctc gccattgaaa accgcttatt tattaacggt gaatatactg ctgcggcgga
     2221 aaatgaaacc tttgaaaccg ttgatccggt cacccaggca ccgctggcga aaattgcccg
     2281 cggcaagagc gtcgatatcg accgtgcggt gagcgcagca cgcggcgtat ttgaacgcgg
     2341 cgactggtca ctctcttctc cggctaaacg taaagcggta ctgaataaac tcgccgattt
     2401 aatggaagcc cacgccgaag agctggcact gctggaaact ctcgacaccg gcaaaccgat
     2461 tcgtcacagt ctgcgtgatg atattcccgg cgcggcgcgc gccattcgct ggtacgccga
     2521 agcgatcgac
//
LOCUS       DADLTY010000321         2487 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.325, whole genome shotgun sequence.
ACCESSION   DADLTY010000321 DADLTY010000000
VERSION     DADLTY010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.325"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..>2487
                     /locus_tag="JN334_004287"
     CDS             <1..>2487
                     /locus_tag="JN334_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY7440199.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQE"
BASE COUNT          728 a          577 c          602 g          580 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggag
//
LOCUS       DADLTY010000322         2439 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.326, whole genome shotgun sequence.
ACCESSION   DADLTY010000322 DADLTY010000000
VERSION     DADLTY010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.326"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..565
                     /locus_tag="JN334_004288"
     CDS             <1..565
                     /locus_tag="JN334_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7440200.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            523..1200
                     /gene="bglJ"
                     /locus_tag="JN334_004289"
     CDS             523..1200
                     /gene="bglJ"
                     /locus_tag="JN334_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HAY7440201.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="JN334_004290"
     CDS             complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="JN334_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HAY7440202.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2167..2403
                     /locus_tag="JN334_004291"
     CDS             2167..2403
                     /locus_tag="JN334_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HAY7440203.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          628 a          534 c          627 g          650 t
ORIGIN      
        1 agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta
       61 ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta
      121 ttcccaggct gtagaactgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc
      181 cattgagaat ctggtcaaga ccgtacgttc cggcaatacg cacgcagagc gtatcagcgc
      241 catgctgacc tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct
      301 cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat
      361 agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg
      421 tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc
      481 aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt
      541 aagaagagaa atgtcgcact catagaaaaa tgcgtcatga gtagtatcgg tattgagagt
      601 ttattcagaa agtttgcggg caacccttat aagctccata cctataccag tcaggagtca
      661 tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg
      721 agaagtgagc gcagagaggg attatcttgc ctgactgagc tggcgattaa gtttccgcgt
      781 acccggcgtt tagttattgt ggatgatgat attgaagctc ggctgattgg ttcattgtcg
      841 ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa
      901 ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac
      961 attaaccaaa gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt
     1021 ggctactcaa tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg
     1081 cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc
     1141 gaagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa
     1201 tccgcatgat gccgggttta cttccccggc agtgctttca tttcagcgta caatagccac
     1261 attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt
     1321 cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa
     1381 aaaagagggc atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag
     1441 tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc
     1501 cggtcgcttc tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc
     1561 gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg
     1621 cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt
     1681 tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg
     1741 cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat
     1801 gatcggaata gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg
     1861 tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat
     1921 gtttggcgat catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg
     1981 ttcgccagat gacatcttca tagagcggtg cggaacgata ggccataatc gtgatagtaa
     2041 tctaaatgat aatgattgct aatcctagcg ataggtttac tcgatagcaa gggatttctc
     2101 tggcttgcaa atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat
     2161 gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg
     2221 attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg
     2281 ggattagtat tgacgccggt gatgctgtat cacaaacagt tgcggcatta cattttgcta
     2341 ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga
     2401 tgacaaaaca gggaagaaac aagaaaggaa gtaaagata
//
LOCUS       DADLTY010000323         2437 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.327, whole genome shotgun sequence.
ACCESSION   DADLTY010000323 DADLTY010000000
VERSION     DADLTY010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.327"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..360
                     /locus_tag="JN334_004292"
     CDS             <1..360
                     /locus_tag="JN334_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY7440204.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="JN334_004293"
     CDS             361..726
                     /locus_tag="JN334_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7440205.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1406
                     /locus_tag="JN334_004294"
     CDS             723..1406
                     /locus_tag="JN334_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HAY7440206.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
     gene            1605..1680
                     /locus_tag="JN334_004295"
     tRNA            1605..1680
                     /locus_tag="JN334_004295"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ile,seq:cat)
     gene            1682..1758
                     /locus_tag="JN334_004296"
     tRNA            1682..1758
                     /locus_tag="JN334_004296"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Lys,seq:ttt)
     gene            1761..1836
                     /locus_tag="JN334_004297"
     tRNA            1761..1836
                     /locus_tag="JN334_004297"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1794..1796,aa:Thr,seq:tgt)
     gene            1840..1914
                     /locus_tag="JN334_004298"
     tRNA            1840..1914
                     /locus_tag="JN334_004298"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1873..1875,aa:Gly,seq:tcc)
     gene            2207..2422
                     /locus_tag="JN334_004299"
     CDS             2207..2422
                     /locus_tag="JN334_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY7440207.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          640 a          520 c          653 g          624 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attattggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc gcccggccct ttagctcagt
     1621 ggtgagagcg agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca
     1681 accgccacta gctcatcagg aaagagcgtc aaccctttaa gttgagtgtg cgaggttcga
     1741 gtccccggtg gcggtccagt gccgacttgg ctcagtaggt agagcaactg acttgtaatc
     1801 agtaggtcac cagttcgatt ccggtagtcg gcaccatatg cgggcatcgt ataatggcta
     1861 ttacctcagc cttccaagct gatgatgcgg gttcgattcc cgctgcccgc tccagtcaga
     1921 gtctttcagt ctgcgatgat gggaaatccc ggagtgactg aaagacgttt aagttatgaa
     1981 tgatcgcttt tttttgcaaa attgctgtgc agaaatacta accttcgggc aggcgatcat
     2041 tcataagcac tctgctttta ttccgattaa ctgtgagtgg tttgttggat agagtgcttt
     2101 ccttattgta tatatcgttt cgcccgcttt tgcggttttt tcttttcaaa tccctttcat
     2161 ttctcagtgt aaaactacgc catccgttat ttgcggaggt gaggctatga aatccatgga
     2221 caaaatttca acgggcattg cctacggcac ctccgcaggc agtgctggct actggttttt
     2281 acagtggctt gatcaggtca gtccgtcaca gtgggctgcg attggtgtac tggggagtct
     2341 ggttctgggc ttcctgactt atctgacaaa tctgtacttc aaaatcagag aagacaagcg
     2401 taaggctgcg agaggtgaat aatgtcgcca tcattac
//
LOCUS       DADLTY010000324         2419 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.328, whole genome shotgun sequence.
ACCESSION   DADLTY010000324 DADLTY010000000
VERSION     DADLTY010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.328"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..244
                     /locus_tag="JN334_004300"
     CDS             <1..244
                     /locus_tag="JN334_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY7440208.1"
                     /translation="AENDALRRKLDNGGRVLVKGKCPVPSSAETSSASGMGNDATVEL
                     SPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(333..845)
                     /locus_tag="JN334_004301"
     CDS             complement(333..845)
                     /locus_tag="JN334_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7440209.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1040..1804
                     /locus_tag="JN334_004302"
     CDS             1040..1804
                     /locus_tag="JN334_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440210.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1755..>2419
                     /locus_tag="JN334_004303"
     CDS             1755..>2419
                     /locus_tag="JN334_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAY7440211.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          682 a          540 c          614 g          583 t
ORIGIN      
        1 agctgaaaat gatgctcttc ggcgcaagct tgataatggt ggtcgggtgc tcgtcaaagg
       61 aaaatgccct gtgccatcct cagccgaaac ctccagcgcc tccggcatgg gcaatgatgc
      121 caccgtcgaa ctctctccag ttgctggacg aaacgttctc ggtatccggg acggaatcat
      181 cagcgaccag acagcattga gaatgcttca ggagtacatc aggatccaat gccttggggg
      241 gtagcggtaa ttttactcat tatccttcaa atcagattct gttgtcagag gaatggggga
      301 agctggattc gcaagagctt tttatgggca gtttagctaa aaaaaggcaa cacgaagcta
      361 aaactaccaa cataaagtgc tgaccagcta gcagctgaaa tcagactcac tgtatacact
      421 ttcactggat gaagttgcaa cattcctgcc accaaaggag caatgtaacg cagcacggcg
      481 ataaagcgcg aagtgaaaag tattgacaca gagttatttt gcaactgaag gcgaacccgt
      541 tctattgtat tggaacggct cgatattata catgcaagtt gagggatatg cccaattatt
      601 ataccaagat ggtaattaac gatagtaccg caccatgcgc cactcatgac cgtgattcca
      661 gcttcccatg gtgaaagagt cgtcttgctg actgtaatta cagtggccat cataattgat
      721 gctggtggca atatcgccga aatgagtaaa gtggattttg tgaacgctat tataaaaaga
      781 agcccccata ggcgatatgg atgaagtaca aaatagttca tcaaagcatt tatccactcc
      841 atcaatgtct ttccttctgt gaatttttct ttgctattta accgtgatac gacttaattt
      901 aggcttaaac accttacatt tacaaggtac tcctgtgggg ggccctgcca cggagcgtcg
      961 caggagtgag tgatgaggaa gaattaaagc aacctattgt gacaggcagc gataacagta
     1021 aatcacagga agcataatca tggcaaaacc ggactgggag gccatcgaat cggcataccg
     1081 ggccggagtc cttagcctcc gtgatatagg cgagaaatac ggcgttacag aaggggctat
     1141 caggaagagg gccaaaaagc ttgattgggc acgcagtggc ggtacgcagg tttgcaaaaa
     1201 tggtacgcaa aaaaggaaag tgcgtaccag cagaaagcct gccattactg gccttacaca
     1261 aaaaagtacg caactaaaaa cagaatctac accggatacg aaaccgatac gcggaatgcg
     1321 taccgatccc ccgactaacc cattccaacc cggtaaccag caggcattaa aacacggtgg
     1381 ttatgcccgt cgccttctgc tcaaagatga ggtgatagag gacgctaaag cgttgacgct
     1441 cgaggacgaa ttatttcgcc ttcgtgctaa caaccttgtc gctgcagaga atattggtcg
     1501 gtggttggtg tcgctggaag atgctaatgg ggaccaggaa aggaagatgc tgattgaaaa
     1561 tatcagcgcc gccgagaaag caatgatgcg caatacagtt cgtattgagt ccatcgttgg
     1621 cacgcttgcg acggtaggaa aaatatttgc tgatacagcc taccgcaagg ccgccactga
     1681 taaggtgtct ctggaggctg atcgtcttcg ccgtgatgca ggtattgatg atggcaatgg
     1741 agagcgtgac ctcaatgact tctactctga catccaaacc gacgctgaat ccggctttac
     1801 gtagtttctg gactatgcgg gcacgtaaca aagtgcttta tggtggtcgg tcatcgtcaa
     1861 aatcatggga tgccgctggc attgccatat ttctgtcgaa taaatacacc ctgcgttttt
     1921 gttgtgcccg tcagatccag aataaaatcg aagagtcggt gtataccctg ctcaaaattc
     1981 agatagacag gtttggtctg cggcaccgtt tccgtattct gaacaacaaa atcattaacc
     2041 gggttactgg ctcggaattt gttttttatg gattatggcg caacatcgaa gaaattaagt
     2101 cactggaggg gatcgatatg ttgtggctgg aagaagccca cgcactgacg gaataccagt
     2161 ggaaaattct ggagccaacg atccgtaaag agggttcgga atgctggttc atattcaacc
     2221 ccggacttgt tactgatttc gtctggcgca acttcgttgt tgatccgccc gaaggcactc
     2281 tcatccgcaa aattaactat gacgaaaatc cgtttctgtc tgacaccatg cttaaggtta
     2341 tcgacgcggc gcgacgccgt gatccggatg gttttaaaca tgtgtatgag ggcgttccgg
     2401 agtctgatga tgatgcggc
//
LOCUS       DADLTY010000325         2371 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.329, whole genome shotgun sequence.
ACCESSION   DADLTY010000325 DADLTY010000000
VERSION     DADLTY010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.329"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..823)
                     /locus_tag="JN334_004304"
     CDS             complement(<1..823)
                     /locus_tag="JN334_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7440212.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1422..1670)
                     /locus_tag="JN334_004305"
     CDS             complement(1422..1670)
                     /locus_tag="JN334_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY7440213.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2025..>2371)
                     /locus_tag="JN334_004306"
     CDS             complement(2025..>2371)
                     /locus_tag="JN334_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7440214.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          611 a          437 c          574 g          749 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggtgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggggggc aatttttcct taaggtatga aaaaaggagc
     1081 tgaagctata taatagctat taatgccact ggtatcagtg aatcatgtat ataaaaaact
     1141 gtgttaagga ggctgtccat tagcggtttt ttattcttct ggaaccgcgg catctttgcg
     1201 tatctttttg tcttctcacc gtctggtcgg tgttttgctg agactgctaa tttcctgtct
     1261 ttgctagtgc tgtactggta ctgcccaatc atgattggcg ggggcgggcg gagttgagac
     1321 cgcagacacg tcgtatgcaa gaacgtgctg cggctggctg gtgaacttcc gatagtgcga
     1381 gtattgaatg atttccagcc gttactgatt ttacgtgcta attagtgaac aaaccactcg
     1441 tcagcagact cccaggtatc tttcagagtt tcctgaacaa aagttttagc tgaatcttta
     1501 tcggcggtgc gcgtaacaga aaggccatcg ttgctggtgg cttttacgat cacctctaca
     1561 tcgtcataac gtttactgat gcgtcgggtt aattcttcct ttaacgcatc cacagcaccg
     1621 gttggcattt tagtcatttt ctctttggct atgcagattt caaaacgcat aaaaatccct
     1681 ctgcactgtg tttttataca gtattatttt taactgtatg gataaacagt gtcaagagtg
     1741 agggaaagtg attttttggg gcatgcatgg ggcaaaaata tttgttgtgg ggcattttgg
     1801 ggcatgattg ggacactttg ttacatatga actttgccga ttttcatatg acatggcaat
     1861 ccaacgcttt gattattcta aagaaaacac atgctcttgg gcgttcttta gtgattttta
     1921 aaaatcccgt atcacgcagt taaagtggcg ggcatactct tcaaggctgg tgatccccaa
     1981 ccgcacccat ttaggatgtg accattgcgg tagaccgatg taaatcataa cgcgtgaagg
     2041 atctcttcca cgctacgcac acgggcgatg cgcgggtaga tatgattaat gctgttattg
     2101 tgttgctcgg cgctggcggc actacaggcg tcttcggcaa tcaccagatt aaagccaagt
     2161 tcccaggcat tgcgggcggt ggattcaaca ccgatattgg tcgagatccc acataacact
     2221 attgtatcga tacctcggcg gcgcagttgc aactccagat ccgtaccgta aaacgcaccc
     2281 cactgacgtt tgatgatttc gatatcactg tcagttgcac ctaatgcagc tggatgttgc
     2341 caccagtttt cgggcaacac tttagcgggg g
//
LOCUS       DADLTY010000326         2325 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.330, whole genome shotgun sequence.
ACCESSION   DADLTY010000326 DADLTY010000000
VERSION     DADLTY010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.330"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            61..282
                     /gene="kilR"
                     /locus_tag="JN334_004307"
     CDS             61..282
                     /gene="kilR"
                     /locus_tag="JN334_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HAY7440215.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            276..452
                     /locus_tag="JN334_004308"
     CDS             276..452
                     /locus_tag="JN334_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440216.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="JN334_004309"
     CDS             527..802
                     /locus_tag="JN334_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440217.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>2325
                     /locus_tag="JN334_004310"
     CDS             902..>2325
                     /locus_tag="JN334_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAY7440218.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          719 a          531 c          529 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatgttcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggta
//
LOCUS       DADLTY010000327         2321 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.331, whole genome shotgun sequence.
ACCESSION   DADLTY010000327 DADLTY010000000
VERSION     DADLTY010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.331"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            215..379
                     /locus_tag="JN334_004311"
                     /pseudo
     CDS             215..379
                     /locus_tag="JN334_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            873..1451
                     /locus_tag="JN334_004312"
     CDS             873..1451
                     /locus_tag="JN334_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440219.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1560..1818)
                     /locus_tag="JN334_004313"
                     /pseudo
     CDS             complement(1560..1818)
                     /locus_tag="JN334_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1802..2321
                     /locus_tag="JN334_004314"
                     /pseudo
     CDS             1802..2321
                     /locus_tag="JN334_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          741 a          420 c          575 g          585 t
ORIGIN      
        1 gctggctcga atacggtggt gcggctgaga gtcgcagagg ggaagttgcg tccatgttga
       61 ggggaacgga gcaaaaaacc atcagagata cattgaatca atcaatgtaa gcaatattaa
      121 gtgtctatac cgattacttg atgatcaggt tgtgttgaat aaacgcgcat tcttcggtgc
      181 ttttttaatc ttttggggat ttaaattcct atcgatgaac tgtcgatatg aaaaagcgag
      241 catcatactg acgtcaaaca aaggggtcgc agactgggga gaaatgttcg gagatcacgt
      301 gctggcaaca gcgatcctag atcggctgct acacgctgaa tatcaaagga gagagttacc
      361 ggttaaaaga gaaacgtaaa gctggagtgc tgaccaaaaa cacaacgcca atcagtgatg
      421 atgaaatggt ggaaaacgga cagcatcagt aacgaaagta tcttagcagg catgaaaatc
      481 gtccgcatcc cctaaacggt caaaatgctt ctgccgtgaa ggaaggagaa tgccatctca
      541 tcacttatat ctggcgaatg aaacacagat ggtcggcctt gcccccaaac acacggcggt
      601 gcaaaatgga atggcagaaa acttcgtcaa aacgatgaaa tatgattaaa tcagtgttat
      661 gttcgaacct gacgtgttaa cagcagtaaa gtagtctcgc gaaggtgttc aaacattata
      721 atgaatggca tcctcatagt gcgcagggtt atcgcttgct gcggaaatat ctgaagcgac
      781 gtaccagtaa tggggaaaat gatagctggt ttctgaaaat atagaggaaa atgtgatata
      841 tatatcctgt taatatttaa ggagaatata aaatgaaagt ctcgtttaag tctttaggct
      901 atatttttca tgatatatat aataaaaaac acactatcga tgagttcaac gatgtggtaa
      961 gaaaagcagt tttatcaggt aaaattaacg agctgaatgc atgtcataaa gtggcaattt
     1021 ttcttgccga aaaagacaat gagataacaa agaaagacaa agccaagata atagatacat
     1081 taactgaaaa ctattccatt gaatttcaac aattaatgaa tattagtgag cgcacgttaa
     1141 attcatcttt atatattact cctggtgaaa gtggttttgt cagttttgtt aatcgtgaag
     1201 gaaaaatatg tcatactgca tatgtaaaat catcagataa tagcatgaca tattaccatg
     1261 caaatggtag cagcattgac aaatatatta cggatatgtg cggccttatt tgtatgaggc
     1321 atattgagtc tactggcatt attttttata tgcttgatga aaaagtttta tctgccattg
     1381 ctgaatttat gaatgaaaaa ggatggcggg ctgcgttttg ttcagcaaag aacttatata
     1441 aatgtgttta attttagaaa aactatttgg taaaatccaa caagagtcga gaggttcgcg
     1501 ttgcttcagc agggcctcag cctgttcaac cagactcagt tgcaggtcgt gacgcagatt
     1561 tagtggtggt ggttgattgt cagataccag ccgatgatct tgtagtgcat atcatcagac
     1621 tttgagaagc atatggtttt gcgggtaagc cgcttaagat gtgtccgcag gttcagatta
     1681 tgtcgctcta tccgctgagt gcatttcttg cttataacat gactggttga ggacaataaa
     1741 gttcggtata ccggctcagg catcggtcat gtagaaggca atgctaaact ggctcagcaa
     1801 actaagagca gcgcgcatcc acttacccaa tgcggttgag aaaatagcct tcgatcgctt
     1861 tcatgtggcg aaacaaccgg gtgaggtggt ggataaaacc cgccagaatg aacatccgca
     1921 tcttcctgtt gaaagtcgtc gtcaggcaaa gggaactcgc ttcctgtggc agcacagcga
     1981 taaatggatg accgaatcca ggcaggaaaa gctgatatgg ctgcgtgcac agatgaagct
     2041 gacgagcctg tgctgggcgc tgaaagaact ggcaaaggat atctggagca gaccatggag
     2101 cgaggaaaga cgcaacgact ggcagagatg gctgcggcca acagtgacgt ccccatgatg
     2161 aagaatgtgg ctaaaacgat agggaaaagg ttgtacggta tcctgaacgc catgcgccat
     2221 ggcgtatcaa acggaaatgc ggaagcactg aacagcaaga tcaggctgct gagaataaaa
     2281 gccaagggct accgcaaccg ggagcgcttt aagctgggag t
//
LOCUS       DADLTY010000328         2295 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.332, whole genome shotgun sequence.
ACCESSION   DADLTY010000328 DADLTY010000000
VERSION     DADLTY010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.332"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..558
                     /locus_tag="JN334_004315"
     CDS             <1..558
                     /locus_tag="JN334_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAY7440220.1"
                     /translation="QPMKLALTGVALSACWASLTDYLMLSRPQDVNNALLWLTGSLWG
                     RDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLGVSVPHTRFWALLLAVAM
                     TSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTGALLLVVADLLARIIHPP
                     LELPVGVLTAIIGAPWFVWLLVRMR"
     gene            559..1326
                     /gene="fecE"
                     /locus_tag="JN334_004316"
     CDS             559..1326
                     /gene="fecE"
                     /locus_tag="JN334_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HAY7440221.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1884..2141)
                     /locus_tag="JN334_004317"
     CDS             complement(1884..2141)
                     /locus_tag="JN334_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440222.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
BASE COUNT          514 a          603 c          614 g          564 t
ORIGIN      
        1 cagccgatga agctggcgct caccggcgtg gcgctttctg catgctgggc cagcctgacg
       61 gattatctga tgctctcgcg cccgcaggat gtgaacaacg ccctgctgtg gctgaccggc
      121 agcttatggg gccgtgactg gagctttgtg aagattgcca tcccgctgat gattttattt
      181 ctgccgctga gcctgagttt ttgccgcgat ctcgacctcc ttgcactcgg cgatgcgcgc
      241 gccaccacgc tcggtgtgtc ggtgccccat acccgattct gggctttgtt actagctgtc
      301 gccatgacat ctaccggcgt ggccgcctgc ggcccgatta gctttattgg tctcgtggtg
      361 ccgcatatga tgcgtagcat caccggtgga cgtcaccgca gactgctgcc tgtttcagcc
      421 ctgacaggtg cgttgctgtt ggtggttgcc gatctgctgg cgagaattat tcatccccca
      481 ctggagctcc cggttggcgt gctgaccgcc attatcggtg cgccgtggtt tgtctggttg
      541 cttgtgagaa tgcgataaat gactttacga actgaaaatc tgacggtcag ttacgggaca
      601 gacaaggtac ttaacgacgt ttcactctca ctgccaacgg ggaagatcac cgccctgatc
      661 ggccctaacg gttgcgggaa atcgacgctg ttaaactgtt tttcgcggct tttaatgccg
      721 cagtctggca ccgtatttct cggcgataat cccataaata tgctctcatc acgccagttg
      781 gcccgcaggc tttcgctgct gcctcagcac catttaacgc cagaggggat cacagtccag
      841 gagctggttt cgtatggtcg taatccctgg ctgtcactct gggggcgtct ctccgctgaa
      901 gacaatgcac gagttaatgt cgccatgaac cagacccgga tcaatcatct tgccgttcgt
      961 cggttaaccg agctttccgg cggtcagcgc cagcgcgcat ttctggcgat ggtcctggcc
     1021 cagaatacgc ccgttgtatt acttgatgag ccaaccacct atcttgatat caatcaccag
     1081 gtggacctga tgcggttgat gggcgaactc cggactcagg ggaaaacggt ggtcgctgtg
     1141 ctgcacgacc ttaatcaggc tagccggtac tgcgatcaac tggtggtaat ggcaaacgga
     1201 catgttatgg cgcaaggcac accagaagag gtgatgaccc caggattgct gagaacagta
     1261 ttcagcgtgg aagcggaaat acaccccgag ccggtatctg gcaggccgat gtgcctaatg
     1321 aggtagattg cacaggccgt aagaaccaaa ccacgactga atgaaactgg actggcgcca
     1381 gcaagcctgt tcagactggg gctgaacttt tccggactct gaaagattac caatactcat
     1441 cgtccatccg cttgctttag gctgacaggt tcataatcaa cgcaaaccag agctgtacag
     1501 gcttgggcgc ggctttcaaa ccagtcgtga tcacggcaat caattttgaa ctctgcttaa
     1561 cggacatttc tgtataaccc ttacggcaac gaaaaacgcg aagttaaaat tttagaaacc
     1621 caaaaacgtg acatgactaa gtttagattt caggggggga gatcaaaaaa tttcgctctg
     1681 tgccagagcg gacattcacg gagctggttc attaccaatg aggttgggct tttgaggata
     1741 aatcaatgat cagacgccaa cgtaaatcaa aagcaaccct ggaacggaat tgctaatcca
     1801 gtttctgacc atcgattttt ctaaaaagtg tgcgtttgct actacttagg taggtgcagc
     1861 tttcttaatc accggcagcc acgttataca ggccagttga tggatcgatt gttatcaatg
     1921 atatcttcat gagtcggtgt ctcacccagc ttaccgaagc tggcataaag tgaaggcaga
     1981 cgggcccgtc cttctccctt tttcgccaga gggaaagcgc gaagcatggt ggcagcctcc
     2041 cagtcacaac aataggatgg tgtgcacggc tgctgacgcc atgattcagc gatagagccg
     2101 gaaaatacgg ggtcaaagcc ggtatcatta accagagtca tcgtgacctg tactgcggct
     2161 ggatgatctc ctgctactgc aactgctagg cgaccgcggc tcccttcagg tagtctgttg
     2221 tggtcaacta aaactggcct ccgcgttaga gtttttccag tatcggtttt ctgattcgtt
     2281 tggtggtaac ccacc
//
LOCUS       DADLTY010000329         2261 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.333, whole genome shotgun sequence.
ACCESSION   DADLTY010000329 DADLTY010000000
VERSION     DADLTY010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.333"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="JN334_004318"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="JN334_004318"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY7440223.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          574 a          559 c          668 g          460 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac gccctgcctc t
//
LOCUS       DADLTY010000330         2252 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.334, whole genome shotgun sequence.
ACCESSION   DADLTY010000330 DADLTY010000000
VERSION     DADLTY010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.334"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="JN334_004319"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="JN334_004319"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HAY7440224.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..>2252
                     /locus_tag="JN334_004320"
     CDS             1647..>2252
                     /locus_tag="JN334_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7440225.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENT"
BASE COUNT          609 a          480 c          583 g          580 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg
     1981 cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgtatgc
     2041 tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca ggcaaaccaa
     2101 cggataaacc aaacctagtg tgcggtccgg tacaaatctg ctggcataaa ttcgcccgct
     2161 actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt atggacccga
     2221 aacgcatgat tgaagcctgt gacgaaaaca cc
//
LOCUS       DADLTY010000331         2218 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.335, whole genome shotgun sequence.
ACCESSION   DADLTY010000331 DADLTY010000000
VERSION     DADLTY010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.335"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="JN334_004321"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="JN334_004321"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HAY7440226.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="JN334_004322"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="JN334_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HAY7440227.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2210)
                     /gene="arsR"
                     /locus_tag="JN334_004323"
     CDS             complement(1881..2210)
                     /gene="arsR"
                     /locus_tag="JN334_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HAY7440228.1"
                     /translation="MFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRHLA
                     LLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNCSG
                     DSKNICS"
BASE COUNT          589 a          611 c          555 g          463 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgg
//
LOCUS       DADLTY010000332         2190 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.336, whole genome shotgun sequence.
ACCESSION   DADLTY010000332 DADLTY010000000
VERSION     DADLTY010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.336"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..818
                     /locus_tag="JN334_004324"
     CDS             <1..818
                     /locus_tag="JN334_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAY7440229.1"
                     /translation="TILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAE
                     IVLEGYIDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDE
                     PAVLGVALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVW
                     SFLRQFMYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPV
                     SGLGSKMGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            864..1565
                     /gene="fre"
                     /locus_tag="JN334_004325"
     CDS             864..1565
                     /gene="fre"
                     /locus_tag="JN334_004325"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAY7440230.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(1757..>2190)
                     /gene="fadA"
                     /locus_tag="JN334_004326"
     CDS             complement(1757..>2190)
                     /gene="fadA"
                     /locus_tag="JN334_004326"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="FadA; fatty acid oxidation complex component beta;
                     functions in a heterotetramer with FadB; similar to
                     FadI2J2 complex; functions in beta-oxidation of fatty
                     acids; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HAY7440231.1"
                     /translation="AAAMLVMSESRAHELGLKPRARVRSMAVVGCDPSIMGYGPVPAS
                     KLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINLNGGAIALGHPLG
                     CSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
BASE COUNT          509 a          565 c          599 g          517 t
ORIGIN      
        1 ccacgattct cggtgcagtc acccccgttc cggatacgct ttcagagtat gcgtttgccg
       61 gattgctacg cggcaccaaa accgaagtag taaagtgtat ttccaatgac ctcgaagtgc
      121 cagccagtgc cgaaatcgtc ctggaagggt atatcgatcc tggtgagatg gcgccggaag
      181 ggccgtatgg tgaccacaca ggttactata atgaagtcga taatttcccg gtgtttaccg
      241 tgacgcatat tacccagcgt gaagatgcga tttaccattc cacctatacc gggcgtccgc
      301 cagatgagcc cgcggtactg ggcgtggcgt tgaacgaagt gtttgtaccg attctgcaaa
      361 aacagttccc ggaaattgtc gatttttacc tgccgccgga aggctgttct tatcgtctgg
      421 cggtagtgac gatcaaaaaa cagtacgccg gacacgcgaa gcgcgtcatg atgggcgtct
      481 ggtcgttctt acgccagttt atgtacacta aatttgtgat cgtttgcgat gatgacgtca
      541 acgcacgcga ctggaacgat gtgatttggg cgattaccac ccgtatggac ccggcgcggg
      601 atactgttct ggtagaaaat acgcctattg attatctgga ttttgcctcg cctgtctctg
      661 ggctgggttc aaaaatgggg ctggatgcca cgaataaatg gccgggggaa acccagcgtg
      721 aatggggacg tcccatcaaa aaagatccag atgttgtcgc gcatattgac gccatctggg
      781 atgaactggc tatttttaac aacggtaaaa gcgcctgatg cgcgtttgtt ttgccctatt
      841 tatcgatccg acagagaaag cgcatgacaa ccttaagctg taaagtgacc tcggtagcag
      901 ctatcacgga taccgtatat cgtgtccgca tcgtgccaga cgcggccttt tcttttcgtg
      961 ctggtcagta tttgatggta gtgatggatg agcgcgacaa acgtccgttc tcaatggcct
     1021 cgacgccgga tgaaaaaggg tttatcgagc tgcatattgg cgcttctgaa atcaaccttt
     1081 acgcgaaagc agtcatggac cgcatcctca aagatcatca aatcgtggtc gaccttcccc
     1141 acggagaagc gtggctgcgc gatgatgaag agcgtccgat gattttgatt gcgggcggca
     1201 ccgggttctc ttatgcccgc tcgattttgc tgacagcgtt ggcgcgtaac ccaaaccgtg
     1261 atatcaccat ttactggggc gggcgtgaag agcagcatct gtatgatctc tgcgagcttg
     1321 aggcgctttc gttaaagcac cttggtctgc aagtggtgcc ggtggttgaa caaccggaag
     1381 caggctggcg tgggcgtact ggtaccgtgt taacggcggt attgcaggat catggtacgc
     1441 tggcagagca tgatatctat attgccggac gttttgagat ggcgaaaatt gcccgtgacc
     1501 tgttttgcag tgagcgtaat gcgcgggaag atcgcctgtt tggcgatgcg tttgcattta
     1561 tctgagatat aaaaaaaccc gcccctgaca ggcgggaaga acggcaacta aaactttatt
     1621 cagtggcatt tagatctatg tcgcatccgg caattggtgc acgatgcctg atgcgacgct
     1681 ggcgcgtctt atcaggccta cattggtgcc ggatcggtag accggataag gcgttcacgc
     1741 cgcatccggc aagtggttaa acccgctcaa acaccgtcgc aataccctga cccagaccga
     1801 tacacatcgt cgccagacca aactgaacgt ctttgcgttc catcagattc agcagcgtgg
     1861 tgctgatacg cgcaccggaa caacccagcg gatgacccag cgcgatcgca ccaccattga
     1921 ggttgatctt ctcgtcaatc tgctccatta gcccgagatc tttaatgcac ggcaggatct
     1981 gcgcggcaaa ggcttcgttc atctcaaaca cgccgatatc gctggcagaa agccccgctt
     2041 ttttcagcgc cagcttcgag gccggaaccg ggccgtaacc cataatcgat gggtcacaac
     2101 caacgaccgc catcgaacgc acacgagcgc gcggcttaag acctaattca tgggcgcggc
     2161 tttcactcat caccagcatg gcagctgcgc
//
LOCUS       DADLTY010000333         2183 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.337, whole genome shotgun sequence.
ACCESSION   DADLTY010000333 DADLTY010000000
VERSION     DADLTY010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.337"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            12..203
                     /locus_tag="JN334_004327"
     CDS             12..203
                     /locus_tag="JN334_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAY7440232.1"
                     /translation="MPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVENVVD
                     VYVTHNPKKTEQKAGVSIH"
     gene            227..694
                     /locus_tag="JN334_004328"
     CDS             227..694
                     /locus_tag="JN334_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440233.1"
                     /translation="MYGGSPADIAAAIWRKAPPGIDMNGNTTFTVADKEYDPPYPEYV
                     ISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGR
                     YYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            696..1301
                     /locus_tag="JN334_004329"
     CDS             696..1301
                     /locus_tag="JN334_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HAY7440234.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1288..>2183
                     /locus_tag="JN334_004330"
     CDS             1288..>2183
                     /locus_tag="JN334_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7440235.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          531 a          507 c          609 g          536 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtatccat taacacccgg ttcgctttat gttggcgtgt acggcggcag
      241 cccggcagat atcgcggcgg ccatctggcg taaggctccg ccgggtattg atatgaatgg
      301 caacacaacg ttcactgttg cagataagga gtacgatccg ccgtatcctg aatacgtgat
      361 cagctggcag acactcaaac ccgtcagtct gcatgtcagt gtgacgctga aaaaaagtga
      421 ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg
      481 tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc
      541 cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga
      601 tggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt
      661 gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc
      721 ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc
      781 tttcccccga cagttttatc agtgattttt atggtcttat ctggaacatc gataccgcag
      841 aaaagtatgg tcttgatgtc tggggaaaga ttgtgggtgt cagtcgctgg ctgacggtaa
      901 aggacgattt taattacctg ggcttcagcg agtccaggat ggacaccccg gtaatggatg
      961 atccctgtcc gtttaatcag gcaccgtttt acaacggaaa atcggatacc cggactgttg
     1021 acctgtctga tgctgtatac cggcggctga tactgatgaa agccatgtcg aacattactg
     1081 actgttccgt tccggatatt aacctgatgc tgagatttat gttcggaaaa aaacgccggg
     1141 cttatgttct gaataatggc gggctgagga tgagttacgt ctttgagtcc gcgctctcgt
     1201 tggcagaact ggcgattatc cagtcgtcgg gtgcactgcc atccccgccg ggtgtttatg
     1261 tttcagtagt tttaaaggag tcccgtaatg aaggccagtg ataaaccccg ccagctggcg
     1321 gtcccctttg cgagtaccgg agataaaaac cgtatcccgg acaaggcgac acagcagacc
     1381 agagagagcg gtaatgctgc gtatgattca ggttttcctc cggtgaccat gacagcggtc
     1441 tcagcgggag gtataccgcc acacggcaag gattttaacg gtctgatgta cgatattacc
     1501 gcagcaatac ggttcgccca ggctggcggt ttgtacacgt ataatgccgg ttttgcgggg
     1561 gccattggtg gatatgcaaa aggagccatt ctcgccggag tcgcaacaac agcggtctgg
     1621 ctgaatacca cggacgataa cctgaccgat cctgaaggct ccgacagtgc gggctgggta
     1681 aatcttcttg aggatccgaa aaggatattc ctgcggcaga agaacaatct gtcagacctt
     1741 cagaataaag ggacggcacg ggataatctt caggtttaca gtaaagagca gtcagatcaa
     1801 cgctatgttc atcgggaagg cgataaaata accggagagc tgaaaatccg tggtgttaat
     1861 gcgctgagga ttttcaacga agcttttggc ctgatttttc gtcgttcgga agagtgcctg
     1921 caccttattc ccaccagtga aggtcagggg gaaaatggcg atattggtcc cctgcgcccg
     1981 ttcaccatta atttgcggac gggtgaaata tccatgtcgc ataaagtgtc tgttggcggt
     2041 ggttcgcagg tcaatggtgc gctgggtatc ggcgttcaga acgccctggg ggggaattca
     2101 attgttcttg gtgataatga caccggattt aaacagaatg gagacggtat tctggatgtt
     2161 tatgctaata gtcagcgtgt att
//
LOCUS       DADLTY010000334         2162 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.338, whole genome shotgun sequence.
ACCESSION   DADLTY010000334 DADLTY010000000
VERSION     DADLTY010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.338"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            80..259
                     /locus_tag="JN334_004331"
     CDS             80..259
                     /locus_tag="JN334_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440236.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            311..505
                     /locus_tag="JN334_004332"
     CDS             311..505
                     /locus_tag="JN334_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440237.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1304..1640)
                     /locus_tag="JN334_004333"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="JN334_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          688 a          444 c          450 g          580 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttctgacgaa atcaacatga aaataaccat atatgataat tattataata acggctttaa
     1801 ttggaataca tatattacaa cgtattatat ataattggta ttctgggaac tatattctca
     1861 aaatacagta gaaacgaggt atgtttctgg tggaaaggac agtgggatta aaaagtaaga
     1921 attgataaaa aaaacgccag caacacatag tgccggcgga gggaataccc catggagaaa
     1981 atgtgatgcc tagagcatca tgaatatata attaaaaata gttagcgttg tcaatacttt
     2041 cacaaaaata attttcgtag tatagaaaga tatttttatg catgacctac ctgaatttgc
     2101 tccgggtaga ggttataaat aaaaattgaa tcacgacaaa cacaatattc atagtatggc
     2161 ga
//
LOCUS       DADLTY010000335         2147 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.339, whole genome shotgun sequence.
ACCESSION   DADLTY010000335 DADLTY010000000
VERSION     DADLTY010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.339"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..219
                     /locus_tag="JN334_004334"
     CDS             <1..219
                     /locus_tag="JN334_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440238.1"
                     /translation="QAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRE
                     TEQQIYRQLTDEVLALRLSENGSQLHHS"
     gene            427..696
                     /locus_tag="JN334_004335"
                     /pseudo
     CDS             427..696
                     /locus_tag="JN334_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            693..805
                     /locus_tag="JN334_004336"
                     /pseudo
     CDS             693..805
                     /locus_tag="JN334_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970002.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            948..1538
                     /gene="ospG"
                     /locus_tag="JN334_004337"
     CDS             948..1538
                     /gene="ospG"
                     /locus_tag="JN334_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HAY7440239.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          609 a          464 c          526 g          548 t
ORIGIN      
        1 caggcagaag agcagaaata tgagatgctg gagaatgagt accctcagag ggtggctgac
       61 cggctgaaag catcaggtct gagcggtgat gcggatgcgg agagggaagc cggtgcacag
      121 gtgatgcgtg agactgaaca gcagatttac cgtcagctga ctgacgaggt actggccctg
      181 cgattgtctg aaaacggctc acaactgcac cattcataat cacatcgcat aaaactgtca
      241 gcgccagtga taggacaggt gaaacctgtc aatacgtacc ggcttacgcc gggcttcggc
      301 ggttttactc ctctgccata tgcaacaacg gagtgccgcc ttacatgccg ctggcgcggc
      361 atatcctggt gacgatatct gaacagttat atactgtgta tatacgtggt aatgacgagg
      421 tggcaaatgg cacaggttaa tatgagtgta agaatcgacg cagaactgaa ggatgctttt
      481 atggccgctg caaaaagcat ggaccgtaat ggctctcagt taatccggga ttttatgcgc
      541 cagaccgttg aacggcagca caattcttgg ttccgtgact aggttgcggc aggccgtcag
      601 caactcgaat gcggtgatgt gcttccccat gacatggtcg aatcctctgc tgctgcatgg
      661 cgggatgaaa tgagcaggaa gattgctgat aaatgatgga gatgttctgg accatgctgg
      721 ccatccagga caggaagcgc attcgtagta cattgcggag cagaacctga tggccgccat
      781 tgaaccggac gaacggattg gttattcagc cagcagcctg gcagggcagc cgtataaagg
      841 ccgcaacggt cgtgtggaag gaacatctgg accacacaag gtagcgtgta acgttatttt
      901 gtgtgaaaat ctcttgtgat ttttaaatat cgtaacaaag gtatgacatg aaaataacat
      961 ctaccattat tcaaacacct tttccatttg agaataataa ttctcatgct ggcatagtaa
     1021 cggagcccat tctcggtaag ttaataggtc aggggtcgac agcagaaatc tttgaagatg
     1081 tgaatgattc atctgctttg tataaaaagt atgatcttat tggcaaccag tacaatgaga
     1141 ttctggaaat ggcttggcaa gaatctgagc tttttaatgc tttttatggc gatgaagcat
     1201 ccgttgttat acagtatggc ggagatgtgt acctccgaat gctgcgcgtg cctgggactc
     1261 cccttagtga cattgataca gctgatatcc ctgataatat agagagcctt tatctacagt
     1321 tgatatgtaa attgaatgag ttgagtataa tccattacga tcttaataca ggtaatatgc
     1381 tgtatgataa agaaagtgaa agtttattcc caatagattt tcgcaatatt tatgctgaat
     1441 attacgctgc aaccaaaaaa gataaagaga ttatcgaccg acgattacaa atgcgtacaa
     1501 atgattttta ttcgttatta aacaggaaat atttatagac gtatttgttg atgctataag
     1561 ccagaggctc ttctatgaga atatggggac cattccctcg cgatattaac gtaatatctt
     1621 aattgtatgc accgccagaa tgcgttcagt gcgatcacgg cagcagacag gtaaaaatag
     1681 caacaaacca cccgaaaaac cgccgcgatc gcgtatgata aattttaacc acatgcatac
     1741 ctgtgcaaca aggcgaatca cgcacggatt tccagccaga gacacctgaa acgtaaccgg
     1801 ctcatttaaa ccgtctggtc tgttacctcc ggttccacaa aaataatgtc catcattttg
     1861 aagtggacac tatcgtatgg aataccggac ctgaatcact gaagctttac gccttcattt
     1921 cgaagaacat ttaccacggg ttgtggccgg atgtcgcctg ggcgtaccaa aatcaactgt
     1981 ctgtggtatg ttcgtgcgct tccgcaacgc tggcctttca tggcctctgc ccgcaggtat
     2041 gtcggagcag gaacttgatg cccttcttta cggaagtgcc tccacagtac ctgtcgtact
     2101 tactgaaagc accgttatgc ccaaactccc ggtggtaaaa aaacgtc
//
LOCUS       DADLTY010000336         2115 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.340, whole genome shotgun sequence.
ACCESSION   DADLTY010000336 DADLTY010000000
VERSION     DADLTY010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.340"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            298..551
                     /locus_tag="JN334_004338"
                     /pseudo
     CDS             298..551
                     /locus_tag="JN334_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
     gene            708..2111
                     /locus_tag="JN334_004339"
     CDS             708..2111
                     /locus_tag="JN334_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7440240.1"
                     /translation="MRQLVISITEGLNMSLFTEPKEIERLPSEEIERLYPVLRYRVFI
                     SIFLGYMGYYFVRNTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSK
                     VFLSLGLFLSGLVNFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEH
                     RERITWATLWNLSHNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLI
                     YFIQVDRPVSVGLPPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCI
                     YGSFVYILRYGIVSWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLS
                     VRLFRNSKPLTNTLFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIG
                     LYSMELVPKEAAGAASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFA
                     IIFTLMSRERSLEFEKE"
BASE COUNT          548 a          417 c          487 g          663 t
ORIGIN      
        1 gggttgggga gcaatggaac agtaaacgcc gttaagaagt caatttaaat agccaaaaat
       61 taactagcca atttacattg caggttgtaa tttttaacca ccttttttac gatagccaaa
      121 ttacctatac ccatcccggc cctgccaagc catacccgct atcggcgatg aaaatgcttt
      181 tgtgcgcggt cggagtggtc ccgacgaggg ttttacccga agtcggggcg tatctccgcg
      241 ttagcgggcc gtgagggccg cttacgagcg tgtatgagag acgtacagtg gttttgtccg
      301 ctcacaccag tacatacagg tacggaaata aaaccagtcg aaatgccggt aacgacgatt
      361 actcctgtct ctgatgccgg tggactacaa gattttattt actggcgtcc tgatgcggac
      421 agggctggta tggaacctgt ttatgtgata cttaatgatc ctctggattc cgggcgattt
      481 acgcgtaaac aactaaataa aaatatctta aacatgcaag taattttggt attctcgata
      541 caagaataaa tagtagtaac tactatagct gcaaataatg ttaaaaacat ttctatgatc
      601 atctttacat ttttcatgat tgacaattgt ttttggttaa aaaaagacaa ctaatgagtg
      661 tgataatgca atactttgag tgattatata atgataagta aacaggtttg cgacaattgg
      721 ttatcagtat tacagaagga ttaaatatgt cattattcac agaacctaag gaaattgaaa
      781 gattaccctc tgaagagatt gaacgattat atcccgtcct gcgatacagg gtctttatca
      841 gcattttcct cggatatatg gggtattatt ttgtccgaaa caccaccagt gtgttatccg
      901 gtgtgctgca catgtctgca accgagatcg gcattatctc ctgtgccggt tttttatcct
      961 atggtatatc aaagtttgtt tccggactta tttctgacag aagtaattca aaagtatttt
     1021 tgtctcttgg tttattcctg tccggactgg taaattttct gataggatat attcccggga
     1081 tcatcacatc cgttacgtta ttttcaacaa tgtatttact taacggctgg atccaaggta
     1141 tgggatatcc tcctggtgcc aaaacactgg tgttctggta tgaacatcgg gaaaggatca
     1201 cctgggccac actgtggaat ttatcgcaca atgtcggggg agctctggcc cccgttctga
     1261 ttggtttcag ttttggtttt ttcggcgaca gtgctctgga tcatgcccgg gccgcattta
     1321 tcttcccagg agtactgtgt atggcaatga gtgtgctgat ttactttatt caggtggaca
     1381 gacctgtatc agtggggctt cctcccattg aagaatggaa aggtaacgtc gtttcacacc
     1441 cggctaaagg ccgtgaacaa ggtccgagac tctccattcc tgatatcatc agaaaacata
     1501 tcatccgaaa taataaactc atttactgct gtatctatgg ttcgtttgtt tatattctga
     1561 gatacggtat tgtttcctgg gctcctaagt ttttaagtga cagtctggat gtcggtggta
     1621 aggatatggg aaaactggca tcaatggggg gggggtctgt ttttgaaatc ggtggtgtgg
     1681 ctggtatgct tcttgccgga tacctgtctg tcagactgtt cagaaactca aaaccattaa
     1741 caaatacttt atttctggca cttaccatta ttttattaat cgcgtactgg tacgtgccat
     1801 caggcaatga atatctctgg ctaaactata caatccttat tctgttgggg ctggcagtgt
     1861 atggtccggt gatgtttatt ggtctttatt caatggagct tgttcctaaa gaagctgccg
     1921 gagccgcatc agggctgtca ggcacatttt cctatatctt cggctcaatt gttgcgacac
     1981 tgggaatggg acttgttgtc gattatctgg gatggggggc cacatttatc gtactgatac
     2041 tgtctgctgt ttttgccatc attttcacac tgatgagtcg tgaacgctca ctggagttcg
     2101 aaaaagagta atttt
//
LOCUS       DADLTY010000337         2111 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.341, whole genome shotgun sequence.
ACCESSION   DADLTY010000337 DADLTY010000000
VERSION     DADLTY010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.341"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..726)
                     /gene="ipaH7.8"
                     /locus_tag="JN334_004340"
     CDS             complement(<1..726)
                     /gene="ipaH7.8"
                     /locus_tag="JN334_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HAY7440241.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQ"
     gene            1056..1235
                     /locus_tag="JN334_004341"
                     /pseudo
     CDS             1056..1235
                     /locus_tag="JN334_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1252..1942)
                     /locus_tag="JN334_004342"
                     /pseudo
     CDS             complement(1252..1942)
                     /locus_tag="JN334_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1865..>2111
                     /locus_tag="JN334_004343"
     CDS             1865..>2111
                     /locus_tag="JN334_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440242.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAI"
BASE COUNT          622 a          391 c          476 g          622 t
ORIGIN      
        1 ttgcagaacg cgagtcgaca atggattccc tgaaatatta atcctgtaac ttgaatctaa
       61 ttgtgaaaaa ctctccggaa gagtggttat ctgattaaaa tgaaaataat attccttttg
      121 tctgatattg tttctatcag aaaattgagg aaaagtaacc agattattgc gctgagcaca
      181 gagaatttcc agtttatccg gtaagtcggg taatgttgtc agcctgttat aataaacatg
      241 taaaaatttc aatttttcgg gaagagccgg taagttatat agcctgttat tttcaacaaa
      301 taatgatgtt aaatggtttg gcaaaacagg aaggttttcc agttgattag aacgaacact
      361 taaagttgtt aatgactcag gaagttctgg tatcacagaa agcctgttat tatcagcatt
      421 aagttcttta agaaatgcag gcagctttgg caatgatgtt aatttattat tttctacaat
      481 caaagcagaa atatgctttg gtaattcggg aagagacgtc aaatttaggt ctgataaatt
      541 taatactgct tcttgattct gaaagcactg ggagagtctg ttaaaagcaa tgcctcgctc
      601 ttccccggga gaagagttct tttcccattc agtcaggatt ctcagataat gttcattact
      661 ggttgagttt gagttaatag agggggagca agaaactgat gagtgtgtat tatttacaga
      721 gaacataatg ctaggtcaac cttatatttg tgagaataaa tatttaaaat tgtatattta
      781 tacaaatttc acttaccatt tccgatatta agttttaata gtttccattt tacacataac
      841 agaagttatg ttgtaattta tttatggagg ttttctatgg aattcttttt ttattgtggc
      901 tgcagtcaat aataacactg aattcaacat tccagtgagc aaggaaatgt aagccgagta
      961 agtatatttg ttttacatca tattttagtc aagctcattt attcctatta tagaatgaca
     1021 aattaaaatt actgtaaaaa aacgatacac atttaatacg agggaggccg gcagaaaaac
     1081 ggcttgcggt caggcaggag aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa
     1141 agctccagag gaaaactttg tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc
     1201 tgaatcactg gaatgcactg aatgagtttt gccgggtagt acttccaatc attagaatat
     1261 gtgcttttca atgaaggaac cgatgacttt ttcgtggatt tccacggaac gcgagaagca
     1321 gattgtttta cgtgccagcc gcttgatgcg ggggcggagg gtcaggttgt tacgttcata
     1381 cgttgcgtga agattttgcc ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg
     1441 ccccagtcat cgctggtcac catgccgata cagaacggag tcaacagggc taacagttcc
     1501 cgacaggtct catcattccg tggcccgcaa gtgtaagcca acacaccgcc agttttagtg
     1561 ttgtacgcat accagggcca gtgttgttgc gccttgctgc caacgaagcg ccactgctca
     1621 tcaagttcac agataagggc aacatcagcg tgagcgactg gcgcagatgt tatccgccgg
     1681 ggcgagagct ttttaatgtc cgtatgacgg tattgatgcc gattttcagt gtcctggcgg
     1741 tatcacggcc ccctgcgccg ttatgtgcca tttcaacgat ctgctcctta acgccgagtt
     1801 ttctggcttc ataagagtaa gtgagctgga aaacacgttt acatgagcga caacggaaac
     1861 gctcatgccg ggaacaacta cggccatgtc gataaacttc atcggaatta cagcgcggac
     1921 aatgaacagt aacggtagtc atggaaaacc tcaaatacca gtagattacc agaaacttct
     1981 atcgattgaa gacattacct tttgccgcaa taggtggggt aacattggtg aaaacgcatt
     2041 acgccgggtg gcagttggaa aaaaattatc ttttttcggc tctgatcgtg gtggagaaaa
     2101 tgcggcaata a
//
LOCUS       DADLTY010000338         2099 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.342, whole genome shotgun sequence.
ACCESSION   DADLTY010000338 DADLTY010000000
VERSION     DADLTY010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.342"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            7..>2099
                     /locus_tag="JN334_004344"
     CDS             7..>2099
                     /locus_tag="JN334_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_051577932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HAY7440243.1"
                     /translation="MQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQR
                     EQTRREHLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSV
                     ILPALNAVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLR
                     QSPAKPNSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFE
                     PQRRYATLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVR
                     LYGRIGQALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESY
                     ATLRRYAPEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQK
                     LVMTDAGIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSS
                     ELPLAVATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPD
                     TAQALIDQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADA
                     INLGLTKMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTT
                     SSSDGQNFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYV
                     LDGLLYHESDLRIEE"
BASE COUNT          438 a          708 c          609 g          344 t
ORIGIN      
        1 ttcgcggtgc agctctgtta cctgcgcttt cccggcgtca tcctgggcgt cgatgaacta
       61 ccgttcccgc ccttgttgaa gctggtcgcc gaccagctca aggtcggcgt cgaaagctgg
      121 aacgagtacg gccagcggga gcagacccgg cgcgagcacc tgagcgagct gcaaaccgtg
      181 ttcggtttcc ggcccttcac catgagccat taccggcagg ccgtccagat gctgaccgag
      241 ctggcgatgc aaaccgacaa aggcatcgtg ctggccagcg ccttgatcgg gcacctgcgg
      301 cggcagtcgg tcattctgcc cgccctcaac gccgtcgagc gggcgagtgc cgaggcgatc
      361 acccgtgcta accggcgcat ctacgacgcc ttggccgaac cactggcgga cgcgcatcgc
      421 cgccgcctcg acgatctgct caagcgccgg gacaacggca agacgacctg gttggcttgg
      481 ttgcgccagt ctccggccaa gccaaattcg cggcatatgc tggaacacat cgaacgcctc
      541 aaggcatggc aggcactcga tctgcctacc ggcatcgagc ggctggttca ccagaaccgc
      601 ctgctcaaga ttgcccgcga gggcggccag atgacacccg ccgacctggc caaattcgag
      661 ccgcaacggc gctacgccac tctcgtggcg ctggccaccg agggcatggc caccgtcacc
      721 gacgaaatca tcgacctgca cgaccgcatc ctgggtaagc tgtttaacgc tgccaagaat
      781 aagcatcagc agcagttcca ggcgtcaggc aaggccatca acgccaaggt acgtctgtac
      841 gggcgcatcg gtcaggcgct gatcgacgcc aagcaatcag gccgcgatgc gtttgccgcc
      901 atcgaggccg tcatgtcctg ggattccttt gccgagagcg tcaccgaggc gcagaagctc
      961 gcgcaacccg atgacttcga tttcctgcat cgcatcggcg agagctacgc caccctgcgc
     1021 cgctatgcac cggaattcct tgccgtgctc aagctgcggg ccgcgcccgc cgccaaaaac
     1081 gtgcttgatg ccattgaggt gctgcgcggc atgaacaccg acaacgcccg caagctgcca
     1141 gccgatgcac cgaccggctt catcaagccg cgctggcaga aactggtgat gaccgacgcc
     1201 ggcatcgacc ggcgctacta cgaactgtgc gcgctgtccg agttgaagaa ctccctgcgc
     1261 tcgggcgaca tctgggtgca gggttcacgc cagttcaagg acttcgagga ctacctggta
     1321 ccgcccgaga agttcaccag cctcaagcag tccagcgaat tgccgctggc cgtggccacc
     1381 gactgcgaac aatatctgca tgagcggctg acgctgctgg aagcacaact tgccaccgtc
     1441 aaccgcatgg cggcagccaa cgacctgccg gatgccatca tcaccgagtc gggcttgaag
     1501 atcacgccgc tggatgcggc ggtgcccgac accgcgcagg cgctgataga ccagacagcc
     1561 atggtcctgc cgcacgtcaa gatcaccgaa ctgctgctcg aagtcgatga gtggacgggc
     1621 ttcacccggc acttcacgca cttgaaatcg ggcgatctgg ccaaggacaa gaacctgttg
     1681 ttgaccacga tcctggccga cgcgatcaac ctgggcctga ccaagatggc cgagtcctgc
     1741 cccggcacga cctacgcgaa gctcgcttgg ctgcaagcct ggcatacccg cgacgaaacg
     1801 tactcgacag cgttggctga actggtcaac gctcagtttc ggcatccctt tgccgggcac
     1861 tggggcgatg gcaccacatc atcatcggac ggacagaatt tccgaaccgc tagcaaggca
     1921 aagagcacgg ggcacatcaa cccaaaatat ggcagcagcc caggacggac tttctacacc
     1981 cacatctccg accaatacgc gccattccac accaaggtgg tcaatgtcgg cctgcgcgac
     2041 tcaacctacg tgctcgacgg cctgctgtac cacgaatccg acctgcggat cgaggagca
//
LOCUS       DADLTY010000339         2089 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.343, whole genome shotgun sequence.
ACCESSION   DADLTY010000339 DADLTY010000000
VERSION     DADLTY010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.343"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..124)
                     /locus_tag="JN334_004345"
     CDS             complement(<1..124)
                     /locus_tag="JN334_004345"
                     /inference="COORDINATES: protein motif:HMM:NF025079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HAY7440244.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQ"
     gene            complement(127..687)
                     /locus_tag="JN334_004346"
     CDS             complement(127..687)
                     /locus_tag="JN334_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7440245.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(813..1163)
                     /locus_tag="JN334_004347"
     CDS             complement(813..1163)
                     /locus_tag="JN334_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HAY7440246.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(1366..>2089)
                     /gene="intI1"
                     /locus_tag="JN334_004348"
     CDS             complement(1366..>2089)
                     /gene="intI1"
                     /locus_tag="JN334_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY7440247.1"
                     /translation="LPWLQEIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLY
                     GTGMRISEGLQLRVKDLDFDHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWW
                     LKDQAEGRSGVALPDALERKYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTF
                     QRAFKRAVEQAGITKPATPHTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHV
                     LKVGGAGVRSPLDALPPLTSER"
BASE COUNT          422 a          636 c          614 g          417 t
ORIGIN      
        1 gctgtcggat gatcgagagg tcggtatcgt tgaatgtgta atgtcggatc aggtcgtcct
       61 tggagtccgg caacgccagc aggctttccc gctcggcggc ggacaggatg gaacgacgtg
      121 gcatatttac tgatccgttc tcaagtattg atacagggtt tcgcgactga ttccgaattc
      181 acgagcaagc ttggtctttt gctcgccagc ctcgacacgt tggcgcagtt cggcaatacg
      241 ctcagacgac agggatttct tcctgccacg gtaagccccg cgttgcttgg cgagcgcaat
      301 accctcgcgc tgacgctcgc ggatcagggc gcgctcgaac tcggcgaacg cgcccatcac
      361 cgagagcatc aggttcgcca tcggagagtc ttcgccagta aaactgaggt gttccttgac
      421 gaattcgata tgcacgccgc gttgtgtcag cgtttgcacg atccggcgca aatcatcgag
      481 attgcgcgcc aggcgatcca tgctatgcac caccacggtg tcgccggtgc gggcgaagct
      541 tatcagcgct tccagttgcg gacgcttgac atccttgccg gatgccttgt cgctaaaagc
      601 gcgatcaacc ttgacgcctt ccagttgccg ttccgggttc tggtcgaagg tgctgaccct
      661 gatataccca atgcgctgtc cagtcatgga attccctgca aaatgtcagg gaagactcta
      721 tgaccttcaa cgagatatgt caataaattc aaaattcaat cctatcctga cgcaatttac
      781 acatggcatc tgacatcagg ttagggtatg cctcaacctg acggcggcga atcacaagcg
      841 tccggtttga cgctggtttc ggtcgcagtg ctggcccgcg cctggaagcg ctgatagcac
      901 tccagcccgc agaaatgctc cacgtactcg gccccttccg gcgtgaaggc ggcatcgagc
      961 gggatttcct tgcagcacac gcagcaactg gtgctcggtt cattggcgtt catggtggtg
     1021 ttcctccatt ggttgacgaa gccgacgaag gccgccgccg gcatcggcct ggcgaacagg
     1081 aaaccctgca ccgtgtcgca acccgcctgc cgcaaccacg caaggcagtc gggtgtttcc
     1141 acaccctcgg ctaccacctc cattgtcgtt ttcagaagac ggctgcactg aacgtcagaa
     1201 gccgactgca ctatagcagc ggaggggttg gatccatcag gcaacgacgg gctgctgccg
     1261 gccatcagcg gacgcaggga ggactttccg caaccggccg ttcgatgcgg caccgatggc
     1321 cttcgcgcag gggtagtgaa tccgccagga ttgacttgcg ctgccctacc tctcactagt
     1381 gaggggcggc agcgcatcaa gcggtgagcg cactccggca ccgccaactt tcagcacatg
     1441 cgtgtaaatc atcgtcgtag agacgtcgga atggccgagc agatcctgca cggttcgaat
     1501 gtcgtaaccg ctgcggagca aggccgtcgc gaacgagtgg cggagggtgt gcggtgtggc
     1561 gggcttcgtg atgcctgctt gttctacggc acgtttgaag gcgcgctgaa aggtctggtc
     1621 atacatgtga tggcgacgca cgacaccgct ccgtggatcg gtcgaatgcg tgtgctgcgc
     1681 aaaaacccag aaccacggcc aggaatgccc ggcgcgcgga tacttccgct caagggcgtc
     1741 gggaagcgca acgccgctgc ggccctcggc ctggtccttc agccaccatg cccgtgcacg
     1801 cgacagctgc tcgcgcaggc tgggtgccaa gctctcgggt aacatcaagg cccgatcctt
     1861 ggagcccttg ccctcccgca cgatgatcgt gccgtgatcg aaatccagat ccttgacccg
     1921 cagttgcaaa ccctcactga tccgcatgcc cgttccatac agaagctggg cgaacaaacg
     1981 atgctcgcct tccagaaaac cgaggatgcg aaccacttca tccggggtca gcaccaccgg
     2041 caagcgccgc gacggccgag gtcttccgat ctcctgaagc cagggcaga
//
LOCUS       DADLTY010000340         2085 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.344, whole genome shotgun sequence.
ACCESSION   DADLTY010000340 DADLTY010000000
VERSION     DADLTY010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.344"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(19..285)
                     /gene="ospE2"
                     /locus_tag="JN334_004349"
     CDS             complement(19..285)
                     /gene="ospE2"
                     /locus_tag="JN334_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HAY7440248.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(467..868)
                     /locus_tag="JN334_004350"
                     /pseudo
     CDS             complement(467..868)
                     /locus_tag="JN334_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1297..2085
                     /gene="virF"
                     /locus_tag="JN334_004351"
     CDS             1297..2085
                     /gene="virF"
                     /locus_tag="JN334_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HAY7440249.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          641 a          370 c          389 g          685 t
ORIGIN      
        1 acgagtctga aattaacttc agaaatcaga ataccgttgg ctttgttttt catcaattcc
       61 ggcttcattt atctttgtca accattgcat ttctgtagca gtgaatttca acgttttcgt
      121 ccggttggca gcaggtattt gtaattcgta tatagctgct tttttattga aaacagtata
      181 tccatctgta gtaacagtag aagataataa tacaggattt gatacttcaa ggatgatatt
      241 ttcttgtttt tgtggaaggc aagggaatat agtttgtgta agcatattgc ttccttatgt
      301 tatattttag taaagaattt attttttaca ttatagtcgg ctaaatgaag tttactgtaa
      361 aaaaacgata cttttttcgt aagcgatgag atgtggagcc agaaaaatgg ctgcgttacg
      421 tcattgaact gtccttgacc cacgatattc agacagtcgt tgtatcttaa gttgacatta
      481 cccgctgacg tcgatattcc cttggtgaga gataacccaa cgcactatgc ggatggtttt
      541 cattgtaatg ctcgatcgcc actgcaaggt tatgcaatgc cgttcttggt ttcggcatga
      601 acgcgatgca gtcttccttc atcgttttca tgaaccgttc tgctattcca ttactctgag
      661 ggctactgac tgccgtcgtg cagggctcca gattcaactc tctggcgaac tgccgtgttt
      721 catgagccgt ataagccgaa ccgttatcgg ccactggatt ggccgttccg gtcctttatc
      781 gccatatcgc tcctgacatg acatcctgca ccgttgcttt gtcataatct cccgtgcctg
      841 ctgcccagtc tatggcttca cgatcgcaat atggaacgta acgctgtcat cgggagaatc
      901 gtcaatgcgg cattcggtta acgcttacca atatcaacac caaccggatt tgtggagcct
      961 ccagtctgaa ggggctttat gcgttccgta taggatatta tgatgctgga gttttgcgaa
     1021 gctccactcc tttccgaaca tttacacaga tattgcctaa gaaaagtgaa accataatat
     1081 ggttatagtc cctttcagtg caaatactta gcttgttgca cagagaaata gaagctgcat
     1141 aagctctttc ttcaaaaaat gtaaataaag ttaaatatag gaaaaattac ttaatctatc
     1201 ttaataacgg aaagattttg tatacaatca ctgttacaga aatttcttag ttactctgta
     1261 aacactaaat atagtttggt atattctgtt gaatttatga tggatatggg acataaaaac
     1321 aaaatagata taaaggttcg cttgcataac tatattattt tatatgcaaa aaggtgttca
     1381 atgacggtta gctcaggcaa tgaaactttg actatcgatg aagggcaaat tgcttttata
     1441 gagcgaaata tacaaataaa cgtctccata aaaaaatctg atagcattaa tccatttgag
     1501 attataagcc ttgacagaaa tttattatta agcattatta gaataatgga accaatttat
     1561 tcatttcaac actcctattc tgaggagaaa agggggttaa acaaaaaaat attcctcctc
     1621 tctgaggagg aggtttctat cgatttgttc aaatctataa aagagatgcc tttcggcaaa
     1681 agaaagatct atagtttagc ttgcctttta tcagctgttt ctgatgagga agctttatat
     1741 acttcgatat cgatagcttc ttctcttagt ttttctgatc agataaggaa gattgttgaa
     1801 aaaaacatcg agaagagatg gcgtctttct gatatttcaa ataacttgaa tttatcagaa
     1861 atagctgtta gaaaacgatt ggagagtgaa aaattaacat ttcaacaaat ccttcttgat
     1921 attcgcatgc atcatgcagc aaagctttta ttgaatagtc aaagctatat taatgatgta
     1981 tcaagactta tcggaatatc aagcccatct tattttataa ggaaatttaa tgaatattat
     2041 ggtataactc caaagaaatt ttacttatat cataaaaaat tttaa
//
LOCUS       DADLTY010000341         2084 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.345, whole genome shotgun sequence.
ACCESSION   DADLTY010000341 DADLTY010000000
VERSION     DADLTY010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.345"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            1..796
                     /locus_tag="JN334_004352"
                     /pseudo
     CDS             1..796
                     /locus_tag="JN334_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1235..2072
                     /locus_tag="JN334_004353"
                     /pseudo
     CDS             1235..2072
                     /locus_tag="JN334_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          515 a          504 c          631 g          434 t
ORIGIN      
        1 gggccttgcc cgtagcagtt atttttatca ccgggctgca ctgaaagccg gtgataaata
       61 cgccacgata cgtaagatgc tgacagatat ctttaacagt aattaccagt gttatggcta
      121 tcgtcgcctg catgcgatgc tcaggcatga gggggggcgg ctatcagaaa aggttgtacg
      181 cagacttatg gtggaggaac agcttgtcgt cagtcgtaac cgtcgtcgtc gctacagctc
      241 atattgcgga gaaatcggac cggctccgga taaccttatc gccagagatt ttaaggcgga
      301 gcaacctaat cagaaatagc tgacagatat cacggagttc cagctccctg caggtaaagt
      361 ctggctgtca ccagtggtgg actgcttcga tggaaaagtt gtgagctggt ctctcagtac
      421 acgccctgat gctgaactgg tcaacactat gctggatagc gctgtcgaaa cgttaaatgc
      481 tggcgaacga ccggtgatac acagtgacag aggtgggcat tatcgctggc caggctggct
      541 ggaaagagtg aatgcagcag gtcttattcg ctcaatgtcc cgtaaaggat gttcacctga
      601 taatgctgca tgcgaaggct ttttcggcag actgaaaacg gaaatgtatt atgggcgtaa
      661 atggtcgggc atcacgccag aaaagttcat gcagcaagtg gatgcttaca tcagatggta
      721 taacgagcgg cgtataaaat tatcgctggg tgccgtcagc cccaaaatgt accgccaaca
      781 atgcgggctg gaatgataaa gcagtccagg aaatcgtccg catcccccct tcaccatttg
      841 gattgtccgc tccacccaac atgttgtttc cttaaggttc ctacaccaca aaggaaactc
      901 tcatgctgcg cagtggatgg ctgacgttgc cgacaggcgg gaacttcgcc agttcaggca
      961 gacaccggaa cagcgcttca cgcaggaaca ggagcatctg cagccgctgc tgggtacgga
     1021 cttcgatatc cgtcatgtct cctgggatgg ctatatcgag gttggcggga accgttacag
     1081 tgtgccggaa agcctgtgtg gtcagctggt atcaatacgg atctcgctgg atgatgaact
     1141 gcggatctac agtaatgagc aacaggtagc atcgcaccgt ctctgttcgg ctgcatacgg
     1201 ctggcagacc gtgccggagc atcacgctcc actctggcag caggcttcgg agcgcatggc
     1261 cgaatggctg ggggagatac agaaacgggt aatcactgtc tgtgaccggg aagcggatat
     1321 ctggcattac ctgtattata aagtcagcca cggacagcgt ggtgcgtgct gcacagaatc
     1381 gccggctgga agaggcaccc ggcaagctct tcgaactgcc ggaagtcctg gcaaccgcag
     1441 gaagccacac gctgaatgtg atgcaaaaag gcgggcgggc agcccgtcag gcccggatgt
     1501 tcatcagtta cagcgaagtc agcataaaaa atcccgacaa cagcggccag gcgctcccgc
     1561 tcacgtatgt ctgttgccgg gagcaggcag aggacggtgc ctgctggcat ctgctgacgt
     1621 caggaaaagc ggcgagtgcc gccgatgcac gacgtattgt cagccattac gagcgacgct
     1681 ggctgacaga ggaataccac aaggcgtgga aaagtggtgg tacatggaat cgctgcgaat
     1741 gcagacccgg gataaccttg agcgcatggt ggttatcctg gcgtttatag cggtacgggt
     1801 gctgggcctg cggcagggag gtgtcagtga agaaacacaa aatgacagct gtgagaaaat
     1861 actgacgccg acagagtgga aactactgtg ggttaagctg gaaggaaagc cgcttccggt
     1921 gcaggcaccg accctgaaat gggcctgggg cgatggcatg acagcaaacg cacaggtcgt
     1981 cccggttggg gcgtcatgtg ggatggctgg tcagacttca ggatatggtt gagggttacc
     2041 tggtgatgaa gtctcttgat caggagctct gatcaagaga cagc
//
LOCUS       DADLTY010000342         2066 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.346, whole genome shotgun sequence.
ACCESSION   DADLTY010000342 DADLTY010000000
VERSION     DADLTY010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.346"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..545)
                     /locus_tag="JN334_004354"
     CDS             complement(<1..545)
                     /locus_tag="JN334_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440250.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
     gene            complement(1207..>2066)
                     /locus_tag="JN334_004355"
     CDS             complement(1207..>2066)
                     /locus_tag="JN334_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440251.1"
                     /translation="TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSV
                     LVPVRGGKRPEVVTAPSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALN
                     ICDNVIRTINSKMVDVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEV
                     SDYGIIAHESHLKALSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTAL
                     SFGCFRHDGESITVVLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVK
                     RLSFTKEAN"
BASE COUNT          566 a          472 c          480 g          548 t
ORIGIN      
        1 attccatcca gtcgttttgg gttgctaatg atgtcctgca tccgcttaat ctggtcgggc
       61 gtagacatat aaccccacag ggtgagcaga accttttttg cggctacact atttccttgg
      121 tctgatacta atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg
      181 gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa gaataaacgt tccccagggt
      241 ttctccgtca acgttaactt tcgtgtgttt tctgttaatg ttagcaatcc ttgagtttcg
      301 tctttggtat agtgactctt tatttcattt tttaatttgg ctgagtgaat cagaagacga
      361 taattaggat acttatctgg atttaacgat aaataacttt tatctgttgc tgcccccctg
      421 gaattaagaa tgttaaatac agattcgctt gtcacttctg cctctgtcgc ctggcatgtg
      481 ccgcacgaaa atataccagc gagcagaaga gttaatgcag atttcccttt cataaaataa
      541 tccatcaatc gtttgttaaa agcgtagata accacttatt caacaacttg aaaataaggt
      601 attttacctg agttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa
      661 atggggaagt gttacagcga ttgcgaaaag cctgaagtga ttgaattaag ataatataga
      721 aaatcagata cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag
      781 ggagcctacg acaaatcgaa gacaaccagc taacctattg tttcttaaag acaggtgaat
      841 ccagttggtt gtagtgatag tcacacattt agagatagaa ggtcaagact tgaagcacgt
      901 aatattccct ctaaaggaat agaactcacg gtctgactac ccggcccttt tggtctggct
      961 caggtcaggc tatgggggct tttggctcgc tctttatatt agatgaggtc tcacattttt
     1021 cagtcaaaac tcatcgtcta atagcccata gccagaccac cagtcccagc tttagcagga
     1081 agaccacgag atagactttg tgtaggtcca tttggccctc ctactttctg tattcacaaa
     1141 ctagattact agtacaaaat aaaggccaga catccagttc ggaagtctga ccaatagtcg
     1201 ttattgttag ttagcttctt tagtgaagct caggcgtttg actacaggca ggacagcagt
     1261 agccgagcca gtacccatct tgatggtcgt agcagcttca gctacaacaa acaccttgcc
     1321 gagaatctca aggtcgtgag actgggagtc tccatcacgt gaaaggacca cagtaatgct
     1381 ctcgccatcg tgacggaagc aaccgaagct cagagccgta aaacgcttag ccatcatgaa
     1441 cacgccacgg tcttcaccgt tgtaataggc gacgtcagtc cccagcatat cgacgatagc
     1501 gtcttcacca ccgaagccct gcttagcagc cagacgggag agagccttga ggtgtgactc
     1561 atgggcgata atgccgtagt cagaaacctc agaaccgaaa gaagactccg tgttctcgta
     1621 cagggcatcc atgatagcgt cagccttagc ggtcaggcta ccagtcaggg ccggaagttc
     1681 tacggtcttc agcagctttg aggactccag cacgtcaacc atcttagagt taatggtacg
     1741 aatcacgtta tcgcagatgt tcagtgcgtc ctgctggagc gtcccctgag tgaaaccagt
     1801 ggtcgacttc tggtcccaaa gctttgtttg gtgagacacg aggatcgcct gttcaatcgg
     1861 ctcaacggtg atttcagtgc cagacggagc ggtcacaact tcaggtcgct taccaccacg
     1921 gacagggacc agcacagagc ggtcagccag agtgtgagca atgattggag tcggtaagct
     1981 gttacgggtc ggcaggactt ctacagggaa cgggatgtta tcttgagcgt ctttaagtac
     2041 accgttcagc atagccttaa cagtct
//
LOCUS       DADLTY010000343         2060 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.347, whole genome shotgun sequence.
ACCESSION   DADLTY010000343 DADLTY010000000
VERSION     DADLTY010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.347"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(238..402)
                     /locus_tag="JN334_004356"
     CDS             complement(238..402)
                     /locus_tag="JN334_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440252.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1304..1588
                     /locus_tag="JN334_004357"
     CDS             1304..1588
                     /locus_tag="JN334_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY7440253.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1576..>2060
                     /locus_tag="JN334_004358"
     CDS             1576..>2060
                     /locus_tag="JN334_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7440254.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          529 a          434 c          588 g          509 t
ORIGIN      
        1 ttctcggggc gcttaccttc aaaatgatgg acattatttt tgtggaaccg gaggtaacag
       61 accagacggt ttaaatgagc cggttacttt aaacgggtga tggacagcac ctcaaaagtt
      121 cagatggatt atatttgtct gcaatacccc gggtattttc gatttgcgaa atggatggaa
      181 cgacttgctc aggggatagc tgatggagtt actgaagtac cgaaggacca ctaaaaatca
      241 caggccggga atttcggcgt ttccggcgta ggtgtgccac gaatgcatcc agtggcgtga
      301 tgttggatgc aatgctgtat gagagatgag gggcggcgtc cagacgcagc cttcaaaccg
      361 ccttgagctg ctgccgttaa gagcggtggt ggtgagcgtc aaggttaaaa ggcgcagaac
      421 atgtgtcagg taggtgttgc ggagatgaac gaaagtgaac tgtcgatgaa gtgtcgaaag
      481 gaacctctag gcgatgtcga aaatgtgcta tctacgcagc gcatgacgag tccagcagat
      541 atctggtact ggctgggcgg catccggcat ataggcagca tgaacgcaac acaggctctc
      601 gtacggaacg tgggaacctg tcgtctcgat gctaagggag cggcgcggag cagaggcact
      661 gcaagcgcct gagtaccgat gcgagacaca ggggcagatg gcttcgtagt agtgaggaag
      721 tgcctgtaat gggcatggag cgaagggggt cagtcgttgg ggctggttta tcggtcaacc
      781 ggatagccgg gaggagccca tgagtcagcc aaagccgttc gaggtctcta agtatgcggt
      841 ttggaaagca taccagcggg tcaaagctaa tcgaggtgcg gcaggtgtcg atgggcagtc
      901 agtcgaggcg tttgaggtta ccaatcgaag ctatacccgg taatggcgca ggttgacctg
      961 cacttggcca agtggattgc ccgcaaacac aagcgagcgc gaggtagtct ggtgcgtgca
     1021 ttcgagtggt tactgcgaat tcgtcacgac tgcccgacgc tgtttgcaca ctggtgctta
     1081 gcttacgata cttgaatggt tcaacgacaa gagccgtatg acgcgagagt gtcacgtacg
     1141 gctccgtggg agcgtagggg ggcggtgccc ctgcgccacc cgatatcccc tttgagtgcg
     1201 gttacgtcca cagaggtaaa tgccgctgac gcggcatctg taatctgtta ctttgtgtat
     1261 atcatttgct atatattgtc aatatgctat tgttgtaaag caaatgatat acggaggctt
     1321 tatgaaatca ggtgttcagc tcaatctcag agcaagagaa tcacagcgga tactcattga
     1381 tgctgctgca gaaattcttc ataagtcgcg tacagatttc attctggaaa tggcctgtaa
     1441 agctgctgaa gatgttattc tcgaccggcg ggtgtttaat tttaatgaca ggcaatatga
     1501 agagttcatt gaaatgcttg atgctccggt tgcagatgat cctgccatcg aaaaattgct
     1561 ggcaaggaaa cctcaatggg atgtgtaact gcaccagaac ctttatcctc atttcatcag
     1621 gtcgctgaat ttgtcagcag tgaagctgta cttgatgact ggttaaagca gaaggagcta
     1681 aaaaatcagg caatcggagc cacccggact tttgttgtat gcagaaaagg tacgcaacaa
     1741 atagtcggtt tttactcact ggccactggt agtgtcaatc atacagaagc tacaggtaac
     1801 cttcggcgca atatgccaga ccccattcct gtaattatac ttgcacgcct ggctgtagat
     1861 gtgtcatttc gaggaaaagg gcttggcgcg gatttacttc atgatgcagt tcgtcgttgt
     1921 tacagggttg ccgaaaatat cggtgtgcgt gcaattatgg ttcatgcgct tacggaaaac
     1981 gcaaaacagt tttatattca tcatggtttt aagccatcaa aaactcaggt gcaaacattg
     2041 tttcttaaac tccctcaata
//
LOCUS       DADLTY010000344         2048 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.348, whole genome shotgun sequence.
ACCESSION   DADLTY010000344 DADLTY010000000
VERSION     DADLTY010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.348"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(59..328)
                     /locus_tag="JN334_004359"
     CDS             complement(59..328)
                     /locus_tag="JN334_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7440255.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            385..1053
                     /locus_tag="JN334_004360"
     CDS             385..1053
                     /locus_tag="JN334_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7440256.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1369..>2048
                     /locus_tag="JN334_004361"
     CDS             1369..>2048
                     /locus_tag="JN334_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7440257.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          615 a          448 c          381 g          604 t
ORIGIN      
        1 ttaccagcca accgcagcac gtcctgcata cgtcgtgtct gcggtttttc tttttcgctt
       61 acattgtgtc tggttcttcc ggccactcaa tatcaggtgc agttgatgta tcaacacggt
      121 tcagcaacac ccgatacttc ttccaggctt ccagcaacga gttttcttcc tccgttgcga
      181 tctccagatc tacagcatcc tgaagtggcg caatatgctc actgaatccc tggatgtaga
      241 actgtgtggt gacggtcttc cagccattcg gctcctgctg tatcgaagca taccaggcta
      301 tttcaatatc gctatgctgc ggcagcattt aaccccttgt aattcatcgc cataattgat
      361 ttaattcaca aataaaacta taatatggtg aaattaatga aaaaaaacac agatgatggg
      421 gctaaaattt acacaccact taccctaaag ctttatgact ggtgggtttt gggagtatca
      481 aatcggcttg catggggatg tcctacaaag gaacaccttc ttccacactt tctggaacat
      541 ttaggtaaca accatctgga tattggtgtt ggaactgggt tttaccttac tcacgtacct
      601 gagagtagtc tgatatcttt aatggatttg aacgaagcta gcctgaacgc ggcatctaca
      661 agggctgggg aatcaaaaat taaacataaa attagccatg atgtttttga accttatcct
      721 gcggcgttac atggtcaatt tgattccatt tccatgtttt accttcttca ctgcctgcct
      781 ggaaatatat ctacaaaaag ctgtgtaata cgcaatgcgg cgcaggcctt aactgatgat
      841 ggaactctat acggagccac aattcttggc gatggagttg tgcacaatag cttcggtcaa
      901 aaactgatgc gcatttacaa tcagaaaggc atcttttcaa acacaaaaga ttccgaagaa
      961 ggcttaacac atatactctc agagcatttc gagaatgtta aaaccaaggt tcaaggtact
     1021 gtagtaatgt tttccgcttc agggaaaaaa tagcatccaa ccgcagcacg ttcttgctta
     1081 agacgtgcta cggtttcctt tacgatttgg ggttgcgact ttacctctat tgataatgca
     1141 ttccggcaga acgttcaaat atgaacgtac gatatttaac taaccgaaaa acaaaatata
     1201 gcacaggcga gacatttacc atctaagaaa atagtatcgt ttttttacag ttaaatcaac
     1261 atcacttcct taaaatgaaa acaataaatt tcaatcctga aacattttat ttcagcttat
     1321 tattccccag tatcacattt tatcaggata tccttctgcg aggttattat gcttcctgta
     1381 aataatcccc ccctatccac tggaaacgtc tctttttaca gaactacatc aatcgacaat
     1441 gttcacaata attatctctc cgaatgggtt gaatggacta aaaacagcat ttccggagaa
     1501 aacagggaaa ctgcttttac ccggctccaa ttatgtctgg agaacagtga aacatcgttg
     1561 gacttatctt gtttaggtct cagatctcta ccacgattgc ctgacaatct tgatgaaatt
     1621 aatgtaagca ataaccaact atcaatgctc cccgagctac caagggcatt gaaagagctg
     1681 aatgcaagca gtaatcaatt atctgcactt cctgaattac cagtgtcgct ggaatatata
     1741 aatgtgagtg ataaccattt gttcgcactt cctgaattac ctgcgtcact agaatatatt
     1801 aatgtaagtg acaatcacct gtctgtactt ccgaggttac caatgtcatt ggaattactt
     1861 gatgcagcca gaaatgcttt ggaagtaata ccagattttc cagaaagaga tgatcatatt
     1921 ataagaatat tctggcttaa tcagaaccgg atcacggcaa ttccggaaag catacttggc
     1981 ctcagttctg atagcgttgt caatcttaga gaaaatcaac tatctcccag aataatgcaa
     2041 actttgtt
//
LOCUS       DADLTY010000345         2034 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.349, whole genome shotgun sequence.
ACCESSION   DADLTY010000345 DADLTY010000000
VERSION     DADLTY010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.349"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..362)
                     /locus_tag="JN334_004362"
     CDS             complement(<1..362)
                     /locus_tag="JN334_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002443793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7440258.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /locus_tag="JN334_004363"
     CDS             complement(429..1643)
                     /locus_tag="JN334_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HAY7440259.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          633 a          492 c          414 g          495 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttgaata atcacataac aaactccatt aatcattgaa
     1861 aatgagcaca gaaatagaaa ttaattatat tcaattaatt aaaatgtgta ttgcttgatg
     1921 tatttaaaaa tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag
     1981 gaatgtgaca aataatggtt ctggttgggc gttaaattta aggtcaccag aatg
//
LOCUS       DADLTY010000346         2032 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.350, whole genome shotgun sequence.
ACCESSION   DADLTY010000346 DADLTY010000000
VERSION     DADLTY010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.350"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            9..566
                     /locus_tag="JN334_004364"
                     /pseudo
     CDS             9..566
                     /locus_tag="JN334_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            1086..>2032
                     /locus_tag="JN334_004365"
     CDS             1086..>2032
                     /locus_tag="JN334_004365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7440260.1"
                     /translation="MKETESSYNKKFNSDYKSNNQQTSFDQPDWKTGVFKFDTLHLNN
                     ADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNNGFDFKQTISTNLSIGE
                     TKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVIAQNSMFTTKGIDIS
                     GELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASVTGNIYADDAATITL
                     GQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNNAIWHLNSQSSINRL
                     ETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQA"
BASE COUNT          746 a          357 c          399 g          530 t
ORIGIN      
        1 catacggcat gatgatttat aaaaatgata aaacatttcg taatcttgaa atattcggag
       61 acagcggctc tggagcatac ttatatgata acaaactaga aaaatgggta ttagtcggaa
      121 caacccatgg tattgccagc gttaatggtg accaactgac atggataaca aaatacaatg
      181 ataaactggt tagtgagtta aaagatacct atagtcataa aataaatctg aatggcaata
      241 atgtaaccat taaaaacaca gatataacat tacaccaaaa caatgcagat accactggta
      301 ctcaagaaaa aataactaaa gacaaagata ttgtgttcac aaatggggga aatgttctgt
      361 ttaaggataa tttggatttt ggttgcggtg gtattatctt tgacgaaggc catgaatata
      421 acataaacgg tcagagattt acatttaaag gagcaggaat tgatatcgga aaagaaagca
      481 ttgtaaactg gaatgcattg tattccagtg atgatgtttt acacaaaata ggccccggta
      541 ctctgaatgt tcaaaaaaaa cagggggcaa atataaagat aggtgaagga aatgttattc
      601 ttaatgaaga aggaacattt aacaatatat accttgcaag cggaaatggt aaggtaatac
      661 taaataaaga taattccctt ggcaatgatc aatatgcggg gatatttttt actaaacgtg
      721 gtggcacgct agatttaaat ggacacaatc agacttttac tagaattgcc gccactgacg
      781 atggaacaac aataactaac tcagatacaa agaaagaagc cgttctggca atcaataacg
      841 aagactccta catatatcat gggaacataa atggcaatat aaaactaacg cacaatatta
      901 attctcagga taagaaaact aatgcaaaat taattctgga tggtagtgtc aacacaaaaa
      961 atgatgttga agtcagtaat gccagtctta ccatgtaagg ccatgcaaca gagcatgcaa
     1021 tattcagaag cacagcgagt cattgctccc tggtatttct ttgtggaacg gactgggtca
     1081 ccgttttgaa agaaacagag agttcatata ataaaaagtt caattctgat tacaaaagta
     1141 ataatcagca gacctcattt gatcagcctg actggaaaac cggggtgttt aaatttgata
     1201 cattacacct gaacaatgct gacttttcaa tatcacgcaa tgccaatgtt gaaggaaata
     1261 tatcagcaaa taaatcagct atcacaatcg gcgataaaaa tgcttacatt gataatcttg
     1321 cagggaaaaa tattactaat aatggttttg acttcaaaca aactatcagt actaatctat
     1381 ccataggaga aactaaattt acaggtggca tcactgcaca taacagccaa atagccatag
     1441 gtgatcaagc tgtagttaca cttaatggtg caacctttct gaataatact cctataagta
     1501 tagataaagg agcaaaagtt atagcacaaa attccatgtt cacaacaaaa ggtattgata
     1561 tctccggtga actgactatg atgggaatcc ctgaacagaa tagtaaaact gtaacgccgg
     1621 gtctccacta cgctgctgat ggattcaggc tgagtggtgg aaatgcaaat ttcattgcca
     1681 gaaatatggc atctgtcacc ggaaatattt atgctgatga tgcagcaacc attactctgg
     1741 gacagcctga aactgaaaca ccgactatat cgtctgctta tcaggcatgg gcagagactc
     1801 ttttgtatgg ctttgatacc gcctatcgag gcgcaataac agcccccaaa gctacagtta
     1861 gcatgaataa tgcgatctgg catctaaata gccagtcatc aattaatcgt ctagaaacaa
     1921 aagacagtat ggtgcgtttt actggtgaca atgggaagtt tacaaccctt acagtggaca
     1981 accttactat agatgacagt gcatttgtgc tgcgtgcaaa tctggcccaa gc
//
LOCUS       DADLTY010000347         2025 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.351, whole genome shotgun sequence.
ACCESSION   DADLTY010000347 DADLTY010000000
VERSION     DADLTY010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.351"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            6..224
                     /locus_tag="JN334_004366"
     CDS             6..224
                     /locus_tag="JN334_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 domain-containing protein"
                     /protein_id="HAY7440261.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="JN334_004367"
     CDS             226..591
                     /locus_tag="JN334_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7440262.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..1253
                     /locus_tag="JN334_004368"
     CDS             588..1253
                     /locus_tag="JN334_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440263.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="JN334_004369"
     CDS             1253..1618
                     /locus_tag="JN334_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY7440264.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          553 a          419 c          538 g          515 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcaca
//
LOCUS       DADLTY010000348         1978 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.352, whole genome shotgun sequence.
ACCESSION   DADLTY010000348 DADLTY010000000
VERSION     DADLTY010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.352"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            334..557
                     /locus_tag="JN334_004370"
                     /pseudo
     CDS             334..557
                     /locus_tag="JN334_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            666..932
                     /gene="ospE1"
                     /locus_tag="JN334_004371"
     CDS             666..932
                     /gene="ospE1"
                     /locus_tag="JN334_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HAY7440265.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            1322..>1978
                     /locus_tag="JN334_004372"
     CDS             1322..>1978
                     /locus_tag="JN334_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7440266.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     M"
BASE COUNT          659 a          415 c          336 g          568 t
ORIGIN      
        1 gcttcttacc atatttgaaa taaagctcaa tagcgcgaat tttttgttct tcagtaaaca
       61 tgtttcatct tctgagagtc caggaaatcg tccgcatccc cattaccgtc ttatatcact
      121 ggcgctgaca tgtgctcatt gatgaagtga atctgaatga catgttttgc catatcaacg
      181 ccaaccggtg tatatttcat ttgtggagtc tccagtctga ggacgcttta tgcatcccgt
      241 attgggcagt atgatgccgg aaagctgcga ggctccacac ctgccagttt caccctttta
      301 accatcatac tactccgtgt ttaattgtgt gggatgcgtt cattacattt taacagtgtc
      361 accccgtcgg ccacgaaaga taaacaggtg accagaaaag ggatcttcat ccagcacatg
      421 ctatatcttg aacgtaacaa cctgaccctc cgcacccgca tcaagcggct ggcacgtaaa
      481 acaatctgct tctcgcgttc cgtggaaatc cacgaaaagt catcggctcc ttcattgaaa
      541 aacacatatt ctactgattg gaagcgccac ccgaaaaagt atcgtttttt tacagtaaac
      601 ttcatttagc cgactataat gtaaaaaaat aaatttttta ccaaaatata acataaggaa
      661 gtaatatgct tacacaaact atattccctt gtcttccaca aaaacaagaa aatatcatcc
      721 ttgaagtatc aaatcctgta ttattatctt ctactgttac tacagatgga tatactgttt
      781 tcaataaaaa agcagctata tacgaattac aaatacctgc tgccagccgg acgaaaacgt
      841 tgaaattcac tgctacagaa atgcaatggt tgacaaagat aaatgaagcc ggaattgatg
      901 aaaaacaaag ccaacggtat tctgatttct gaagttaatt tcagactcgt ttaagctcca
      961 ttttcaaaaa aaatatatca gggtttgtta ctaataactt ctgaaaacag aaaaaaatag
     1021 agcagtaatt ctatttcaga gaggagtaac tccatctatc ctcgatacgt actgaacgtt
     1081 atcgtgatat attcatcatg ctttcttcac cattattcga gtatagggag agacgcaatg
     1141 accttaaata cctttttatg gctgaattac cattctcagg aataacagca gactccttcc
     1201 catagacggc ttagggggaa agcaataccc ggagaaaggg tacaaagcaa actgttttaa
     1261 aattaataag ccatattcta tttaactaga attcttatcg ttgataagaa ttctagttat
     1321 aatgattaaa tcaaccaata tacaggcaat cggttctggc attatgcatc aaataaacaa
     1381 tgtatactcg ttaactccat tatctttacc tatggaactg actccatctt gtaatgaatt
     1441 ttatttaaaa acctggagcg aatgggaaaa gaacggtacc ccaggcgagc aacgcaatat
     1501 cgccttcaat aggctgaaaa tatgtttaca aaatcaagag gcagaattaa atttatctga
     1561 gttagattta aaaacattac cagatttacc gcctcagata acaacactgg aaataagaaa
     1621 aaacctatta acacatctcc ctgatttacc accaatgctt aaggtaatac atgctcaatt
     1681 taatcaactg gaaagcttac ctgccttacc cgagacgtta gaagagctta atgcgggtga
     1741 taacaagata aaagaattac catttcttcc tgaaaatcta actcatttac gggttcataa
     1801 taaccgattg catattctgc cactattgcc accggaacta aaattactgg tagtttctgg
     1861 aaacagatta gacagcattc ccccctttcc agataagctt gaagggctgg ctctggctaa
     1921 taattttata gaacaactac cggaattacc ttttagtatg aacagggctg tgctaatg
//
LOCUS       DADLTY010000349         1975 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.353, whole genome shotgun sequence.
ACCESSION   DADLTY010000349 DADLTY010000000
VERSION     DADLTY010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.353"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(1..836)
                     /locus_tag="JN334_004373"
                     /pseudo
     CDS             complement(1..836)
                     /locus_tag="JN334_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT          532 a          465 c          458 g          520 t
ORIGIN      
        1 gctgctctac ggtcatattt ttaattgcgc tccggtttac aagagcccac ccctgttttt
       61 ccagataaaa ctggaaagta tccagggtac agaccagtgc accgtcagga acagtttcgg
      121 tgaatctgat attgccgtgt tcgtcgaagt gcacaaccag agtgcgaccg ttacccggaa
      181 tcatcttgtc agcggacggg gtattattct ggtgcagttc tgcctccatg cggtcgaaca
      241 tcagcgatgt aggcctcttt gaatgcagcg gcttttttcc cagtgaagcc catcaccagg
      301 aaaacgaagc cgtttttggt gatttggtac attgggcgtt tttcgccttt ggtgtcggtg
      361 taggtgacgt cctcaaaatt gagggcgtta aattcaggtg agcattcagt atttgcgatg
      421 gcacgtaaca cattgtcgtg tcgtttgtgg aagaactctg caaccgcaac agacgtagtg
      481 acagcgcgac cgttttcgat ggttacgtca gggtgagaaa gggcagggat agtagccatg
      541 atggcatcct cgagtgataa gttaattaac tcaccaccga ggttttccac gaccataagg
      601 gtggtgagac gtacaggggt ggaaataccg gtcactcgag aacccggcca gtcttgcgac
      661 tgccctgcac atcccaccat aatttgaatg gagctgtgca ttacgcataa aaaaaccgct
      721 tcagcgcggc tatgcgctcg agtaacgttc gggtttccac gcccggcccc cgttttatga
      781 ggtgcagatg cactataatt ccacccgttc tggttttcaa tagctacatt caacattttc
      841 tcttaccttt catcaccgaa gtgaactttg ttgatgcggt gcctggtgcc tccaggtgac
      901 gttaaccagt taacaattaa cgccggatac agggaaaccc ataaccaccc gatacgtggg
      961 aactaatcgc tttttaactg ttccgcgtgc gctcagccgc attcaccgca tcacaaaatt
     1021 cactttaaaa agggcggaca tcagccagca atgaaactga tgccgccaaa ggtaccaatc
     1081 aacatggagt gttgtggcgt ggttgtcact taagcgtatg gtcaacctga caacccggtg
     1141 tcctcaacgg ggaaggaata actccgccat acttaccgcc gcgtcatttc gcgttgtgtg
     1201 cctgctttta actacgtcag gcgaggtggt tcctgttatt ccccaacaac aagaaatttg
     1261 tataatctga ataccccaac aataagaaga gtgagcaagt gatgtattag cgatgtcagc
     1321 tctcaaagag actgcatcaa tgttaaaatt attcaatgaa gcaaaaactc aggctgaggt
     1381 tgatcgcgct gtgtctgaaa taaactctaa gttgtcaagc attcagcttg aatatttgtc
     1441 atctcattga tgagctgaca atctctaaaa gcaatgaggc tgcgctcaaa gcaaaactca
     1501 cggagataga aaagtttaag ttccaaagtg aagactgcga acctcgctaa ttgattactg
     1561 gaacgattgt gtatgccaga cagtgcaata ctagctctgg caagattacc atgtattttt
     1621 gtccagaatg ttttgcgaag agagcaatat ctatattgca acctttccct atcgaggaag
     1681 gcgataaata tacgaaaact caatgcccct cctgtaaaaa tgagtatctc atggactaca
     1741 atttcaataa tgatttttga gtcacggtat tcagattttt aaagaacatg ccggatactc
     1801 acccgtgtcc ggcgcacgca ctccacctca cccgtggaga actccttaat taccaacctt
     1861 ggcttcgttg gttagccatt aacgctggta tgtaatcatt ctggcaatgc ttaatttgaa
     1921 gtggcacact gaagttggcc acctgaacag aggtgatatg ctcacctcag aacaa
//
LOCUS       DADLTY010000350         1924 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.355, whole genome shotgun sequence.
ACCESSION   DADLTY010000350 DADLTY010000000
VERSION     DADLTY010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.355"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(135..1589)
                     /gene="ospC2"
                     /locus_tag="JN334_004374"
     CDS             complement(135..1589)
                     /gene="ospC2"
                     /locus_tag="JN334_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HAY7440267.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          610 a          332 c          300 g          682 t
ORIGIN      
        1 tctccaggat tcccggggcg ttcaccacat gcaatgtgtt gttgcagggg aacggtctgc
       61 accatcccct atatcgatca atataacaaa aatgacaacg tcagattcag ggcacaacct
      121 aaatatattt taggtcacct cccaaatcgt ttgcctgata ccgctccatt ttttaataaa
      181 aaatcaattg tttttgaatc tttgcttttc attgcattgt caagcatagt gtccccacta
      241 tttgcttttt gaaatctttt gtttacatca accaatccat tactaataaa atattctagc
      301 actttatagt tggcactatc ttcactaagt aaatactcaa catcatacag ctcacaatac
      361 atattgttgt tcattttctc catctctgcg atatcttgtt ttgtaaaatt aaatttagat
      421 agtaaataaa gtgccatatc agattttgca ttgcctagag aataagcaag agcttgatga
      481 gccatttttt tatcagtcac cttattgtta atttcttcat aattggatgc tttaatggca
      541 tcctccaaag atatatctct gagtcttatc tttttgaagt tatctgtgct tagcattctt
      601 ggtatatgat actcaagctg aaacacaaag tttataattc tatccagact aacaggatca
      661 atgtttttat tataacaaaa ctctcggaat ctttgatctt tactctttct tataaaatca
      721 ataagatgca acccaattcc aagcctcata tctttagtat taaatattgg cacatcagtt
      781 tttccctgat taccatgcac atacctgcta acttcatcca caacctcttt aaagttatct
      841 aagaaaaatg attggtgctc atggtaaaaa acaggtggaa tagcattatc aaagtggtcg
      901 gttaatgtca tatatccata tggagagtca tgatcaatga tatacgcatt agcaccaaaa
      961 tccacagtat gatgtgtttt atttagtggg agtgtttctt gatgattgga aatttcaacc
     1021 ccaaaaaaaa caaaatcgtg gttagctatt tcttcaatat cctcagagaa tgtgttttta
     1081 tatgtttgta tattctcaga cttaatttta tcaaaggact taatattaag agtattattt
     1141 gacaggttag cgttactttg atgtctgaag ttaaaatcag aattaataat aatagagagc
     1201 aagtgacgtt catccgaaga aagaggatga gttttttgta ttatttcaga aagatagtgt
     1261 ctggacacct tatcgaggtg ctcaagtgac cgaaatgatg accgtgctcc agatggcgct
     1321 tcaatagaaa gatttaatga tttgaaactg gtcccttgag agaacatttt actataactt
     1381 tgcgctgcta tagacttcct taaaaagctt ttcacagtat cagcacagtg atctaaatgt
     1441 ttttcactta tgccagaaga ggatgagttt gttacgcatg atatgttttt ttgtaatgct
     1501 tttgtatctt tgttcggatt tatttttcca tcaacaagat caatattgtt ctgaacatta
     1561 atatgattta ctgcttcagg tattttcata aaacatcccc cattatgtgc gtttttatct
     1621 gcagttattt agaataatat tatctaactt aatattttct tgtatgtatt ataacgaaca
     1681 tattttaata tgttgtacga ttttcactct gaaatcccct gagtttaatc acacttcctt
     1741 tactgaaagg aattattgca acagtattat ttgtttttgg atatatatct tgcgccttgt
     1801 tatgtagtta atgctcagaa tatgatagat aaaatagaga cgcaaaacag ggcatgctca
     1861 taacatgatg ataacataaa aaattagtaa caataaatga aagaatcatg ggcgctcaac
     1921 aaaa
//
LOCUS       DADLTY010000351         1913 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.356, whole genome shotgun sequence.
ACCESSION   DADLTY010000351 DADLTY010000000
VERSION     DADLTY010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.356"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(53..1702)
                     /gene="treF"
                     /locus_tag="JN334_004375"
     CDS             complement(53..1702)
                     /gene="treF"
                     /locus_tag="JN334_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HAY7440268.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          506 c          491 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DADLTY010000352         1886 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.357, whole genome shotgun sequence.
ACCESSION   DADLTY010000352 DADLTY010000000
VERSION     DADLTY010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.357"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..224)
                     /locus_tag="JN334_004376"
     CDS             complement(<1..224)
                     /locus_tag="JN334_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY7440269.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADA"
     gene            complement(221..754)
                     /locus_tag="JN334_004377"
     CDS             complement(221..754)
                     /locus_tag="JN334_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY7440270.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(820..1449)
                     /locus_tag="JN334_004378"
     CDS             complement(820..1449)
                     /locus_tag="JN334_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440271.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1453..1668)
                     /locus_tag="JN334_004379"
     CDS             complement(1453..1668)
                     /locus_tag="JN334_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY7440272.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          489 a          499 c          408 g          490 t
ORIGIN      
        1 gcatcagcta actccttcgt gtatttagca tcgagtgcat cagcatcacg ctggcgctgc
       61 tgcatgtcag taatggttgc gttcgccagc ttcagttctc cggcgttttt gtcgcgctgc
      121 tctttgtagg taatggcgtt atcacggtaa tgattaacag cccatgacag gcagacgatg
      181 atgcagataa ccagagcgga gataatcgcg gtgactctgc tcattgatct atcccccaac
      241 aggctaatgc gctttcctgg tcacgacgaa taacctgtcc atagcagtta tttgaacgta
      301 tgcggcaatc gcgcccaaca tcttttatcc accagcgaat cgcctcgcat gcgcccttac
      361 gatcaccggc attcagccgc ttataaaacg tcgacgggaa acacttaccg gggccaatgt
      421 tataggggca aaatgacgct atacccgctt tctggggttc ggtcagaggt actttaatat
      481 tgcgctccac ccatgccagc gccttatcac gttcaatagc gttaacctgg tcacattttt
      541 ccttcgacag cttcattccc ggtatgacag gcttaccatc caccattgtg gcaccacgac
      601 agatggtcca tatgccagaa ccatcacggt atgccgttgt gtgattaccc tctttttcgt
      661 tcagaaactg gtcaagtatc tggggagcag acgcgcctgc ggcaatcagc gccagaacag
      721 cagccgacag gccgtatttg atttttgcgc tcatggatat ttatcaggat ttatcggcaa
      781 caaataacga gccaacttat atacgccttt taagataagt cagccccggc tggatccagt
      841 catcaggctc tttcttaaag ggggagtatt gaaatcacga agaagagcct cccgcaccgc
      901 agcatccatg tctgcaccac tggcaagtgc ttcaatctct gccgccacct gcaaataccc
      961 catgcaacgg ccaatgcgtt tcataactct ggtgttttcg tctttcaggc atctgatagc
     1021 gagattaata attttcaggc agtgatgatg cgatgcccgg acaggttttc cgttctcata
     1081 aacgacgaac tcaccctctt cacaggaaac cctgaaaggc cgatggggtt cctggtcttt
     1141 atccggtgca gacgcaataa aatcactttt atgctcttcc tctccggcac tgttaaccgt
     1201 aatgacgtac tcaggccgca ggtaagccgc ctctttttct gacaatgcgg ccggtaaaaa
     1261 cttttcctgc aaaaacttca taaaagtctc gtgagcgact ttgtggcagt aatcgtaaat
     1321 cgccgcataa tgctcatcgc ggcgtttgtt ttcatcttca gaactcacca acgccgacaa
     1381 ttttttatta agctcagtga tttcattttc caggtgattg aatcgctgat tcatttcttc
     1441 atgcttcatt atttactctc cccgtgcagc cttacgcttg tcttctctta ttttgaaata
     1501 cagattcgtc agataagtca gaagccccag aagcagactt cccagcacac caatcgcagc
     1561 ccactgtgat ggcctgacct gatccaacca ctgcaaaaac cagtagccgg cactgccggc
     1621 ggaggtgccg taggcaatgc ccgttgaaat tttgtccatg gatttcatag cctcacctcc
     1681 gcaaataacg gatggcgtag ttttacactg agaaatgaaa gggatttgaa aagaaaaaaa
     1741 cgcaaaagcg ggcgaaacga tatatacagt aaggaaagca ctctatccaa caaaccaccc
     1801 acagttaatc ggaataaaag cagagtgctt atgaatgatc gcacgcccga aggttagtat
     1861 ttctgcacag caattttgca aaaaaa
//
LOCUS       DADLTY010000353         1881 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.358, whole genome shotgun sequence.
ACCESSION   DADLTY010000353 DADLTY010000000
VERSION     DADLTY010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.358"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(80..871)
                     /gene="aadA1"
                     /locus_tag="JN334_004380"
     CDS             complement(80..871)
                     /gene="aadA1"
                     /locus_tag="JN334_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY7440273.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(984..1814)
                     /locus_tag="JN334_004381"
     CDS             complement(984..1814)
                     /locus_tag="JN334_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HAY7440274.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
BASE COUNT          459 a          412 c          417 g          593 t
ORIGIN      
        1 caattatgtg cttagtgcat ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc
       61 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      121 gaacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      181 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      241 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      301 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
      361 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
      421 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
      481 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
      541 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
      601 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
      661 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
      721 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
      781 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
      841 tcgatacttc ggcgatcacc acttccctca tgatgtttaa cgtttgaaat aaggggcgcc
      901 gagcgccagc gaggggagcc aaaagcttgc ttttggccgt cccgacttga ttgaagggtt
      961 gggcgatttt gccattagat tttttataaa tttagtgtgt ttagaatggt gatcgcattt
     1021 ttcttggctt ttatgcttga tgttaaattc gaccccaagt ttcctgtaag tgcggacaca
     1081 aaaacatatt tatgtcctga tttgcttata ataaaccctt caaaccatcc gttttgtaag
     1141 gttctatttg ctgtgaatcc tgcaccagtt ttcccataca gttttgtact attatccaga
     1201 tcttgtagat acatgttctc tatggtgttt tctatggctg agtttttaac tgggagattg
     1261 tgattaataa ttttacgcag gaattgaatt tgttcttctg gtgaaatttt taagctactt
     1321 tcgagccatg cttctgttaa tccgttgttt ctttctttat ctccagagaa gtcttgattt
     1381 ccataatcaa aatctttgag ataattcttg attttattta atccaatttt ttgggttatt
     1441 tcttgcgaaa cccaaacaac agaaaattgc atccacgtct ttggtgtatg attgctgttc
     1501 cagatctcca ttcctttggg ggttttatcc catttgaata tggttttctg atctattatt
     1561 tccgcatcaa atgccataag tgataatgcg atcttgaaag ttgaatctgg tgccatttgc
     1621 gttgcacact ttgctttatt gaattgagca atttcagcgt ttgtggatgc atcgtaaagt
     1681 aaaaaacaac cttcagttcc ttcaaataat ggagatgcaa cagtagagat atctgttgat
     1741 gcactggcgc tgctgtagat aatatttgca attattaaaa aaatagcgaa gttgatatgt
     1801 attgtgtttt tcataataag tattggtttg gtaaagggct taattttaac ggctaacaat
     1861 taatgaggct ccgggttcgc c
//
LOCUS       DADLTY010000354         1872 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.359, whole genome shotgun sequence.
ACCESSION   DADLTY010000354 DADLTY010000000
VERSION     DADLTY010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.359"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..63
                     /locus_tag="JN334_004382"
     CDS             <1..63
                     /locus_tag="JN334_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HAY7440275.1"
                     /translation="AAKNTTQNHALVLADWLRSL"
     gene            complement(85..339)
                     /gene="yoaF"
                     /locus_tag="JN334_004383"
     CDS             complement(85..339)
                     /gene="yoaF"
                     /locus_tag="JN334_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HAY7440276.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            522..1547
                     /gene="dgcP"
                     /locus_tag="JN334_004384"
     CDS             522..1547
                     /gene="dgcP"
                     /locus_tag="JN334_004384"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HAY7440277.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            1580..1678
                     /gene="yoaK"
                     /locus_tag="JN334_004385"
     CDS             1580..1678
                     /gene="yoaK"
                     /locus_tag="JN334_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HAY7440278.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            1681..1755
                     /gene="yoaJ"
                     /locus_tag="JN334_004386"
     CDS             1681..1755
                     /gene="yoaJ"
                     /locus_tag="JN334_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HAY7440279.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
BASE COUNT          478 a          435 c          438 g          521 t
ORIGIN      
        1 gcagcaaaaa acaccacgca gaaccatgcg ctggtgctgg ccgactggct acgtagcttg
       61 tgattttagt acggcatccg gcggttattt ttcaccagcc ggatggtccc gtcgccacaa
      121 tgcccattca tcaatcgttt caccgcccgg taatttgcaa ttgttgctga ccccttgcgc
      181 tgtctgcact ggaatgagcg tcccgccctt ctgctggcaa tagaccgacg ccggatttgc
      241 cataccaatc tgcggcggtt taggtgcttc tggctgagaa ggggttgaac aaccagccag
      301 gaccagcaag caaggcagaa caaaactgat aattttcatt tattgatctc acatatttat
      361 ccaagcttag agtatcgcgg tatcgttttg ttttgcagca ctatttttat tacattcact
      421 caaaacatat tacgtcttgt ttcatctttg ttgatgatgt tttatcatgc ctgcaaagat
      481 taaataatca gcacttaccc gccgtatcct ggagttgttc cgtgtcagat cagattatcg
      541 cccgcgtctc gcaatccctt gccaaagaac agtcactgga aagtctggtc cgacagcttc
      601 tggagatgct ggaaatggtc actgatatgg aatcaaccta cctgaccaaa gtggatgtcg
      661 aagcgcgcct gcagcatatt atgtttgccc gtaacagcca gaaaatgcac atcccggaga
      721 attttaccgt cttgtgggat tactcgttat gcaaacgcgc cattgatgaa aactgctttt
      781 tcagcgatga agtccctgac cgttggggcg actgtattgc ggcacgcaat cttggcatca
      841 ccacatttct gagcacgcca attcacttac cggatggatc attctatggc acgctttgcg
      901 ccgccagcag tgagaagcgc cagtggagtg aacgcgcgga acaggtttta cagttattcg
      961 ccggactgat tgcacaatat attcaaaaag aggcactggt tgaacagctg cacgaagcca
     1021 atgctgcgct gattgcgcaa tcgtataccg actcgttaac cgggctaccg aatcggcggg
     1081 cgatttttga aaatctgacg acgctgtttt ccctcgcccg gcatcttaac cataagataa
     1141 tgatcgcgtt tatcgatctg gataacttca aattaatcaa tgatcgtttt ggtcataata
     1201 gtggcgatct gtttctcatt caggttggcg agcgccttaa tacgctccag caaaatggcg
     1261 aagttattgg tcgtctcggt ggtgatgagt ttttggttgt ttcactaaac aacgagaatg
     1321 cggatatttc gtcgctgcga gaacgtattc aacagcaaat acgtggagaa tatcacttag
     1381 gtgatgttga tttgtattat cccggtgcca gtcttggcat agtagaagtc gatcctgaaa
     1441 caaccgatgc agacagtgcc ctgcatgctg ccgatattgc gatgtatcag gagaaaaaac
     1501 acaaacagaa aacacctttt gtcgcgcatc cagcgctaca ttcctgaggc gtattcacat
     1561 ccttttgatt ggtgataaca tgcgaatcgg tattattttt ccggttgtaa tcttcattac
     1621 agcggtcgta tttttagcat ggttttttat tggctgctat gctgccccgg gagcataaag
     1681 atgaaaaaaa caacgattat tatgatgggt gtggcgatta ttgtcgtact cggcactgag
     1741 ctgggatggt ggtaacgtca cctctaaaaa atagcaaagg ctgcctgtgt gcagcctttg
     1801 tgcaatttaa gcgttaactt ttaatcttcc tgtagataaa tagcacgaca atcgcaccaa
     1861 taacggctga ag
//
LOCUS       DADLTY010000355         1860 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.360, whole genome shotgun sequence.
ACCESSION   DADLTY010000355 DADLTY010000000
VERSION     DADLTY010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.360"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(1..344)
                     /locus_tag="JN334_004387"
                     /pseudo
     CDS             complement(1..344)
                     /locus_tag="JN334_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562879.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(344..1264)
                     /locus_tag="JN334_004388"
     CDS             complement(344..1264)
                     /locus_tag="JN334_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7440280.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1402..>1860
                     /locus_tag="JN334_004389"
     CDS             1402..>1860
                     /locus_tag="JN334_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7440281.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVLRCTGNSG"
BASE COUNT          465 a          451 c          433 g          511 t
ORIGIN      
        1 cacacaccca gcacgccaat gcgttcagca tcaacatatg gcagagttac tgcataatcg
       61 ataacacggc taatatcctc tacgcgctgt gtcggatctt ctatccaacg aggctaccca
      121 ccagattctc cctggaaact ggcgtcataa gcgagaacga catagccttt ttcagccaat
      181 gccttgccat agatattgcc agacgtctgc tccttacaac tgccaaaagg atggacgctg
      241 ataatcgccg gatactgttt tgactcatca aaatttggtg gaaactgaat attggcagca
      301 atatgccagg ccatatcact attgcggatt tgaatatgtt tcattacaga gtttcctcaa
      361 agaacggagc taatactgaa attgcttcag ccacataggc tttgccgtca taaagatcca
      421 tatggtttgc gccttcaaca atgtgatagc gtttatcctg gcttgatgcc cgatcgtaca
      481 ggtcgtcact catccatttg ctccctgcct ggctgcccgc cacaatctgc atcggctgag
      541 tcaggtacac ttccgccata tggtaagcat cataggtaat aatctggtta aggctgcgca
      601 aagtagcgta acccggtgct gttggatact gcgcacgagg tgtgtggtaa tactcccagg
      661 cctgacgcag ttcttcgttc ggcgcatcgg cctccttcat tggagccagt ggcataatgg
      721 cgtattcttc gctgctaata tcgctggttc gggcgtttga gccagcttca atgtgtggca
      781 gagcatcaat agatttcaca ttgttttccc aaccattgcg gaacatcgaa ccaatattga
      841 ccgcactaac ggtaccgatg gccttgatgc ggcgatcctg aattgcagca ttggctgtat
      901 atcctgcacc ggcacaaatt cccatcgcac caattcgggt attgtcgaca taagaaagcg
      961 ttgtcaggta atcaatcacg gcactgacgt cttcagtacg aatgtatggg ttttctaact
     1021 gacgcggctc gccgccactt tcaccttgat aagatgcgtc ataagcaata gtgacaaatc
     1081 ccttttccgc cagcttttcg gcataggttc cggccgtttg ctctttaacg cccccacctg
     1141 ggtgagatac cacaattgcc ggatactgac gggtttcatc aaattttgga gggaaataga
     1201 tcactgcaga caaagagatg gttggattat tgctgttagt gaagctgact ttattattca
     1261 tcattcagtt cctcatggag ttgtcggttc gttttgacgg ttggtgatat cactatagat
     1321 attgatcatt aagttgatta gaccctaaat catgattaga cctatcattt aaatgattaa
     1381 tagattgtat ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc
     1441 gtcgttgcag aggagcgtag cttcactcgc gcagcagccc gcctgagcat ggtgcagtca
     1501 gctttaagcc agatagtgcg ccgtatagaa gaacgattgg gattgcggct tctgacgcga
     1561 accacgcgca gcgttgtacc cactgaagcg ggcgagcatc ttttgtctgt tcttggccct
     1621 atattgcatg acatagattc tgccctgaca tccctgagcg atctgcagaa ccgcccatcc
     1681 gggacaatac gtattactac tgtagaacat gcagcaaaaa cgatattgtt accagccatg
     1741 cgcacattcc tgaaatcgca tcctgaaatt gatattcagc ttaccattga ttatggtttg
     1801 accgatgtcg tttctgaacg ttttgatgca ggcgtcctga ggtgtactgg caatagcgga
//
LOCUS       DADLTY010000356         1846 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.361, whole genome shotgun sequence.
ACCESSION   DADLTY010000356 DADLTY010000000
VERSION     DADLTY010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.361"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..655)
                     /locus_tag="JN334_004390"
     CDS             complement(<1..655)
                     /locus_tag="JN334_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-octaprenyl-4-hydroxybenzoate carboxy-lyase"
                     /protein_id="HAY7440282.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     "
     gene            822..1310
                     /gene="rfaH"
                     /locus_tag="JN334_004391"
     CDS             822..1310
                     /gene="rfaH"
                     /locus_tag="JN334_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HAY7440283.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(1307..>1846)
                     /locus_tag="JN334_004392"
     CDS             complement(1307..>1846)
                     /locus_tag="JN334_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="magnesium dependent; involved in quality control of
                     mutated Tat exported substrates; not involved in the
                     Sec-independent protein export system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase TatD"
                     /protein_id="HAY7440284.1"
                     /translation="AQPEVVAIGECGLDFNRNFSTPEEQERAFVAQLRIAAELNMPVF
                     MHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREEMQACVARGIYIGITGWVCDERRG
                     LELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSSRRNEPAHLPHILQRIAHWRGEDA
                     AWLAATTDANVKTLFGIAF"
BASE COUNT          444 a          519 c          454 g          429 t
ORIGIN      
        1 cagaaaccgg gaaacgttcg cccggatgcg ccgcacacca ctcctgataa tccagcgcgc
       61 cgccgcgatg cgacagccag cgcataatca gtttgttttt accaatcagc tgctggcgat
      121 aaatgcccag attctgccgc tctttatgcg ggccgcgcgt tacggtcagc ccccaggtaa
      181 tcagcggcgc ggcatcttcc ggccagcagg tcataatggg aatgcgattg agatcgacgt
      241 catcgccaga gacgattttt tgttggcagg gcgcaccacg cagtcgcttt gtcggcatgt
      301 ttaacacctg cttaaactgc ggcagtttat caaacaggtc gcggaaacct tttggcggct
      361 ccggctcttt cagaaacgcc aataatttac caacttcacg cagcgccgaa acatcttcct
      421 gccccatgcc catcgccacg cgctttggcg taccgaacag gttgcacagc accggcattg
      481 agtagccttt agggttttcg aacaacagcg caggcccacc agcacgcaga gtgcggtcag
      541 caatttcagt gatttccaga tgcggatcca ccgggagcgt gatacgtttt agctcaccct
      601 gctgttcaag cagcgtcagg aagtcgcgta aatcgttata tttcatggcg tccattgtag
      661 cctcttaatc tgcgcccatt atacggcgtt catctttgca atgctgtaaa tttgttaaat
      721 tagcgtgaac tctgacggta taacgcaaac cggggaatat aattaactta gcgtaaagct
      781 tttgctatcc ttgcgccccg attaaacgga taagagtcat tatgcaatcc tggtatttac
      841 tgtactgcaa gcgcgggcaa cttcaacgtg cccaggaaca ccttgagaga caggcggtta
      901 attgcctcgc tccgatgatc accctggaaa aaatcgtacg gggtaaacgt accgcagtca
      961 gtgaaccgtt attccctaac tacctgtttg tggaatttga tccagaagtg attcatacca
     1021 cgaccatcag cgctacgcgt ggcgtcagcc actttgtgcg ctttggcgcg tcgccagcga
     1081 tagtcccatc ggcggtaatt catcagctat cggtatataa accgaaagac attgtcgatc
     1141 cggccccccc ttatccgggt gataaggtga ttattaccga aggcgcgttc gaaggctttc
     1201 aggccatttt caccgaaccc gatggtgagg ctcgctccat gctattgctt aatcttatta
     1261 ataaagagat taagcacagt gtgaagaaca ccgagttccg caaactctaa aacgcaatcc
     1321 caaacagtgt tttgacattg gcatccgtgg tggcagccag ccatgcggca tcttctccac
     1381 gccagtgcgc aatacgttgc aaaatatggg gcagatgggc tggctcgttg cgccgggatg
     1441 atggctttgg cgtgagatcg cgcgggagca gatacggcgc atctgtttcg atcagcaatt
     1501 tctccgccgg aatcaacggc aacaattccc gcagctccag cccgcgtcgt tcatcgcaaa
     1561 cccaaccggt aatgccgata taaattccac gcgccacgca cgcctgcatc tcttcgcgtg
     1621 tgccggtaaa gcaatgaaga accgcaccag gcagtttatc cagccacggc tccagcaatg
     1681 tcataaaccg ctcgtgggca tcgcgacagt gcataaatac cggcatgttt aattctgcgg
     1741 caatgcgtag ctgagcaaca aaagcgcgtt cctgctcttc cggcgtcgaa aagttacggt
     1801 taaagtcgag accacattca ccaatcgcca ccacttctgg ctgcgc
//
LOCUS       DADLTY010000357         1838 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.362, whole genome shotgun sequence.
ACCESSION   DADLTY010000357 DADLTY010000000
VERSION     DADLTY010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.362"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..164)
                     /locus_tag="JN334_004393"
     CDS             complement(<1..164)
                     /locus_tag="JN334_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7440285.1"
                     /translation="MLTSEQHRCHNEKRNFSAEFKRESAQLVVDQKYTVADAASAMDV
                     GLSTMTRWVK"
     gene            350..1192
                     /locus_tag="JN334_004394"
     CDS             350..1192
                     /locus_tag="JN334_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY7440286.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1195..1800
                     /gene="capU"
                     /locus_tag="JN334_004395"
                     /pseudo
     CDS             1195..1800
                     /gene="capU"
                     /locus_tag="JN334_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
BASE COUNT          533 a          334 c          405 g          566 t
ORIGIN      
        1 tgtttcaccc atcgcgtcat tgtggaaagg ccgacatcca tagcgctggc tgcatctgcc
       61 acggtgtatt tctggtcaac gaccagttga gcggattcgc gtttaaactc tgcgctgaaa
      121 tttctttttt cattatggca cctgtgttgt tctgaggtga gcatatcacc tctgttcagg
      181 tagctaaatt tagtgtgcca cctcaaggtt cttagtgagc cgtttttaaa agatatgata
      241 atatattatt gtttataaat tgttgatgtt tgatttagta gtatataaag ttcaccacct
      301 ttattaggct ttccttttat ctctacttac tgggtgccag aaactttaaa ttttaaacgg
      361 agggggcatt ttgtttaaag caaatcatgt tccggtatta atgtatcatc atgtcagcca
      421 ttgcccagga ctggttacac tttctcccga gacattccgg aaacaaatga aatggttggc
      481 tgaaaacaac tggaaaacac tgtcatcaga tgaactggag tttttttatc ggggcggaaa
      541 acttccccgt aaaagcgtta tgttgacatt tgatgatgga taccttgaca actggtttca
      601 ggtatatcct ttattaaacg agtttaatct caaggcacat atttttttaa ttaccagttt
      661 tattggaaat ggtccggttc gtcactctcc tggaaaggaa tattctcatc gcgattgcga
      721 acatcaaata gcaaccggga atgctgataa tgttatgctt cgctggtctg aagtgaacga
      781 aatgttgcaa tctggtctgg tcgaatttca tgttcataca catactcata cacgttggga
      841 taaaaagttc acttcacgag aagaacagtg taaacatctt cggcaggact tattgagcgg
      901 gagagaatat ttaaaaaaaa tgacaggaaa atgcagtaaa catttgtgtt ggccggaagg
      961 ttattataac aaggattata ttcagatcgc tgaagaactt ggattccatt atttatatac
     1021 aacagaaaga agaatgaatg ctcctgctaa aggaactaca cggataggaa gaataagcac
     1081 caaggaacgg gaaaattgcg tatggctgaa aagacgactt ttttattata caacaccgtt
     1141 tttttcatcg ttgctggccc ttcataaggg gccaagatta ccggatgact aataatgaat
     1201 atactattta cggaatcatc accgaatata ggtggtcagg aattacaggc tgttgctcaa
     1261 atgaaggccc tgaagaaaat ggggcattca gttctgcttg tctgcaggga gaacagcaaa
     1321 attgcttttg aagccagtaa gtttggaatt gatatcacat tcgcgttatt tcgaaacagt
     1381 cttcacattc ctactgtatg gagattactc ggaatagttc atagttttca gcccgatgca
     1441 atcgtttgtc acagtggaca tgatagtaat attgttggtt tggtacggtt ttttacctgg
     1501 aaacatccat tcagaattat caggcaaaag acatatttga cacgaaaaac aaaatttttt
     1561 tcaataaatc atttttgcga tgaggtgatt gttcccggaa caaatatgaa gacacatctg
     1621 gagcaggaag gatgtcgaac tcgggttact gttgtgcctc ctggctttga tttccaggaa
     1681 ttatacgttg attcccgaaa cagtttgcct ccgagtgttc tttcctggct ggcgtcccga
     1741 agaggatgcc ccgtaattgc tcaggtagga atgttgcgcc cggaaaaagg gcacgaattt
     1801 aggtgatgct gccaacttac tgatttagtg tatgatgg
//
LOCUS       DADLTY010000358         1831 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.363, whole genome shotgun sequence.
ACCESSION   DADLTY010000358 DADLTY010000000
VERSION     DADLTY010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.363"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..179)
                     /locus_tag="JN334_004396"
     CDS             complement(<1..179)
                     /locus_tag="JN334_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HAY7440287.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITH"
     gene            complement(320..874)
                     /locus_tag="JN334_004397"
     CDS             complement(320..874)
                     /locus_tag="JN334_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY7440288.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(871..1161)
                     /locus_tag="JN334_004398"
     CDS             complement(871..1161)
                     /locus_tag="JN334_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY7440289.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1161..1760)
                     /locus_tag="JN334_004399"
     CDS             complement(1161..1760)
                     /locus_tag="JN334_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY7440290.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          496 c          420 g          470 t
ORIGIN      
        1 ttatgggtga tcggggagag agtttcaaca gagatttgag tggtcataac gataactccg
       61 tacatttgga aattatcgcc accgacgacg ccaatcgaac tggtggcgaa ctgtgcaggg
      121 ttggcgtaac cgggtacgga aaccggcgag cctttcggct cccccacaca gcccgccata
      181 aatcgcgaat gtgactgtgc aaacgatatg aaaaaagacg cgggcgcgtc tcatatcgct
      241 ccgtaaacat ccgggacgcc aatcccgacg ccagattttg ctggcgcgtg aggaatatag
      301 ccccggataa cagattgagt caacaaacgg tttttagatc cccggaagag aatgcatcac
      361 gcatcggtag atagagcata tattcagcaa gatttagcca tacatctacc cgactgcagc
      421 acgtccggaa acaccactca gggtgtgcgt cattcagcaa ttcagccatt ttacgcttag
      481 tcatcccccg cccttcatac cgttgccgga ggacactaat caatccagga tgctctgcca
      541 gcacctcact tatgactcta tcaatacata acgcctctgc atcagtacaa tgcgccagcc
      601 aggtcttttg cttgccattg atcatctctc gcaaaaacgc ttccagctca ggtttctcta
      661 ttcccgcttt tttcatcctg cgcagggctt cattgacggc tgttttcgtc aattttttgg
      721 atgccagcaa ctggttaaac atatttcccg tcttaccgcc gccaatatac gaccagcgcc
      781 cccacatgcg cagttttccc tgaatccaga cactttccag cgtggtgaga cgaaggtgtt
      841 ccccgctttt gcctgtattt gttgggtaaa tcataaataa ccttcctttc tccagatttc
      901 ttgtgtgcga aaaacacctt ctgcatgcat caggcgtaat tcttctttgg tgtaatcgct
      961 ggtttttacc cgcccgtcga ttaaatcgtg gcatgagcta caggcaatcg ctgcctgcat
     1021 atcgtgtggt tttgtcgctg ttccgcacgt ccccgccagc ctgtaatgcg ccagcacaga
     1081 agtttcggga ttgtgattgc agtagccagg aattctgacg gtgcacatct gcccccgcgc
     1141 cgctttacgt aaatccacca ttacgcaaac tccagtaact gcgcggccac attttcgact
     1201 tcctccggag aggaaaattt acggaacagg atccagttcc acagcacatt cagtacagat
     1261 ttataaacct gctgaaactc ggtttcgtcc atgttcgcaa atgcgataga ttttgccctg
     1321 cgcccacgac taccatcagg ataaatatgc tcggtgtaaa atccggcctg aatggttacc
     1381 cactcgcgga aagcgtcaaa cgactttagc aatgccgtat cccgggttct gcatgtcgca
     1441 actgtattaa ggtattgctc tgcggcatca ctcagggctg gagtgtgttc ccgacctact
     1501 gattcgcaca ggtaatcaac gaagcctgat accagttttc gttcgcgagg cgtgatcgcc
     1561 ccaccgaccg gagtccagta atcaaatccc agttgcagga gtttgaaaaa acgcttgtgg
     1621 aatgcgtagt tacgcacacg cttaaagtct gcgtgtatcc actcacctat tttgatttga
     1681 tgcaaaaaat cgcaactctc cggcgtcgcc gggagaagta atccggaaga ggtttgtttg
     1741 accagttgta tatgcgccat cgtagttctc cgctggcgca gtagaatggg tgttcagccc
     1801 gttatgtagt ataccagaat taatgccaat a
//
LOCUS       DADLTY010000359         1808 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.365, whole genome shotgun sequence.
ACCESSION   DADLTY010000359 DADLTY010000000
VERSION     DADLTY010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.365"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            173..1198
                     /locus_tag="JN334_004400"
     CDS             173..1198
                     /locus_tag="JN334_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7440291.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1808
                     /locus_tag="JN334_004401"
                     /pseudo
     CDS             1266..1808
                     /locus_tag="JN334_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          504 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgtt
//
LOCUS       DADLTY010000360         1759 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.366, whole genome shotgun sequence.
ACCESSION   DADLTY010000360 DADLTY010000000
VERSION     DADLTY010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.366"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(1..263)
                     /locus_tag="JN334_004402"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="JN334_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /locus_tag="JN334_004403"
     CDS             complement(378..1016)
                     /locus_tag="JN334_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440292.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1175..1645)
                     /locus_tag="JN334_004404"
                     /pseudo
     CDS             complement(1175..1645)
                     /locus_tag="JN334_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          502 a          433 c          319 g          505 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttacttttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagta ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcgt gtctaaaaag
     1141 tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg actaacagtt
     1201 cccgacaggt ctcatcattc cgtggcccga aagtgtaagc caacacaccg ccagttttag
     1261 tgttgtacga ataccagagc cagtgttgtt gcagccttgc tgccaacgaa gcgccactgc
     1321 tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga tgttatccgc
     1381 cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc agtgtcctgg
     1441 cggtatcacg gccccctgcg ccgttatgtg ccatttcaac gatctgctcc ttaacgccgg
     1501 gttttctggc ttcataagag taagtgagct ggaaaacacg tttacatgag cgacaacgga
     1561 aacgctcatg ccgggaacaa ctacggccat gtcgataaac ttcatcggaa tgacagcgcg
     1621 gacaatgaac agtaacggta gccatggaaa accttaaata ccagtagatt accagaaact
     1681 tctgtcgatt gaagacatca cccgaagttt gaattcattc gaatactttt tacgaggttc
     1741 ttttcgccag tcctgtaat
//
LOCUS       DADLTY010000361         1744 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.367, whole genome shotgun sequence.
ACCESSION   DADLTY010000361 DADLTY010000000
VERSION     DADLTY010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.367"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..353)
                     /locus_tag="JN334_004405"
     CDS             complement(<1..353)
                     /locus_tag="JN334_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7440293.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMA"
     gene            complement(350..940)
                     /locus_tag="JN334_004406"
     CDS             complement(350..940)
                     /locus_tag="JN334_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7440294.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            complement(927..1298)
                     /locus_tag="JN334_004407"
     CDS             complement(927..1298)
                     /locus_tag="JN334_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7440295.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            complement(1310..1711)
                     /locus_tag="JN334_004408"
     CDS             complement(1310..1711)
                     /locus_tag="JN334_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HAY7440296.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
BASE COUNT          392 a          527 c          408 g          417 t
ORIGIN      
        1 gccatctcat tatcacgttg atagttaaaa ccgagtgggt tcagggtgtc gatgagtccg
       61 gaaagtgccg gtacatcatt cagggccggg aaaatggtgc tctccatcca catatccagc
      121 tctgaatccg gtgcctgtgc ccggatgaag acggcaatat gcagaacagc ctgccagtca
      181 tcttcatccg tcatttttcc ggtgtactga gcatcactca gccacaccgc cacggcaggc
      241 agttcctgcg catcaataaa tgccggaagc ccgtcaaaaa acgtggcgct gtctccacac
      301 tgttcccgaa ggcgtgccag cacgctctga cggatttgtg tgtgtcggtt catttactca
      361 gatgtaacct cagttgttgt ttcagggcat accccagctg tttcggcatt tccgcagcaa
      421 tgatgcggtc gcgggcatct tcaaatgcct gtgtcagcgg tccggacagc gggattttca
      481 ctacctccac aggaagacga ttttttctta gcctgccctg atggtcatgc cctgttgcaa
      541 aacgcgcttc aggaagacac ctcagaacat gccagcggcc attcgccagt tgctgaacaa
      601 atgccccccg gaaaaagtat tttcccactc tcagtccttc accagcacgc cgccgtgttg
      661 tgttcagttt gatggcgggc ggattgcccc ggttaacgcg gatcctggcc gtcatttttc
      721 ctgacggact ggctttaaac acccggacac gctgacgtac cagtttcagg gggatccctt
      781 tcacctggtt atcgcccgca acggtattcc cggcaacctg ccgggtggca accgagaccg
      841 ctttctgtgc cacacggttt atcgcccatg cgctggcctg tggcaccata cgggtatcaa
      901 ggctgttcag attgcggatg gcattctcaa gccccttcat aatatcgtcc tgcccttacg
      961 tttcccggca gcccctcacg ccgcaaccgg atataactac atccgccatc atcaggggtg
     1021 atacgatcca cacagaaaga ctcacgacca atcatcagcg aatcattgcg acacagccgt
     1081 aaaatatctg ctgttttcac aaacagggat ggtaaagagt cttcaatacg cacaccgccg
     1141 gccaccagag aaatattttc aggatcatca aaaaccccgg aaatcatgat cccttttagc
     1201 cggcctgacg taatgaccgc tctaatcccc atattgcgaa taatggcctt gtctgcgaga
     1261 tcaagtgcaa catcaaacag attatcgaaa tcagccacgc tgcctcccgt tattgcattc
     1321 tggccaggcc acgttctgtc attccggctg ccacaccggc aaagacacgg aacgccattc
     1381 ccggcggcac aaatgccaga ggttcatccc gcgtggcatg aagtgcatcg gtatgcagcg
     1441 tcaccagtgc cacgactgta accagatcat ccatattctg aatcacggtc tccggctgcg
     1501 ctgatatcac ctcattttca tgtccggtca gcgcattttc cgggtgggca gacttatccc
     1561 ggatgtcagt gtcatccata ttggcaagtt cctcctccag ctcagccaca cgctgcgcaa
     1621 tatctgctgc gcttcccgag atatccggct cacgtcccag cgatgctgcc agttctctca
     1681 gacgtttcag gttatcgtct ttctttgcca tatctgttcc tgtccgaata aaaaacggga
     1741 gccc
//
LOCUS       DADLTY010000362         1741 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.368, whole genome shotgun sequence.
ACCESSION   DADLTY010000362 DADLTY010000000
VERSION     DADLTY010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.368"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..316)
                     /locus_tag="JN334_004409"
     CDS             complement(<1..316)
                     /locus_tag="JN334_004409"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440297.1"
                     /translation="MACDYRYKNRQYHCLSGSYMARSAKPRKRKPAPQRSKLLRYVVK
                     LHEDDFFDEEEAEVLRFDNFDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEI
                     ARC"
     gene            complement(979..1218)
                     /locus_tag="JN334_004410"
     CDS             complement(979..1218)
                     /locus_tag="JN334_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY7440298.1"
                     /translation="MPQGGVISPLLSNIILNEFDQYLNKRYLSGKARKDRWYWNHSIQ
                     RGRSTAVKENWQWKPAVAYCCYADDCVPRRRVLGT"
     gene            complement(1413..1741)
                     /locus_tag="JN334_004411"
                     /pseudo
     CDS             complement(1413..1741)
                     /locus_tag="JN334_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          406 a          481 c          403 g          451 t
ORIGIN      
        1 tgcagcgggc aatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagtt
       61 ttttgtttcc ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat
      121 cgtcaaaatt atcaaagcgc agaacctcag cttcttcttc gtcaaaaaaa tcatcttcgt
      181 gaagttttac gacatagcgg aggagtttgc ttctttgagg tgcaggtttt cgtttgcgtg
      241 gtttagctga acgggccata taactacctg aaagacaatg atattgtctg tttttataac
      301 ggtaatcaca ggccatgaca agccgcagcc gtcaggccgc ctactcgggg cggtttagaa
      361 cgcttattta gtaccagatc ttacttataa taaaggggct tggcagtttg gataaactat
      421 tgtccttatt ccactaatac aaactttttg taaagtatat taaatatact tcatttcata
      481 ctgaaatccc cggaagtatt caatcaaaca attacaccat aaaaggaaga gggggcccca
      541 attaagttgt caagcatgtt tactgcgcaa tcgcttggtg ctccgtccct gtaccccgca
      601 ttggtactct ggcgctcatg gggcttccac ttgcgcttct cccttcacat cggggtgaca
      661 ggtccacgtt ccccacaaga gcctgaatcg acttcacgcc gcttttatgc cggatgccgc
      721 gcaggcagta aacatatccc acaggaacga ctccaccatt gttttgacat catctatatg
      781 ctttcgacac atcatcagcg gttcactttc gttcgtctct tcgaaccata cctgatcggc
      841 tcatgtccga ccttttccca tctcgctcac caccacatct cttaaatgca gcagcagtgg
      901 atggtttggt gcctgcgcct gcacgccgac accaaggggc cttccctcat ctcttgtgct
      961 gcatggctgc acagggactc acgtccccag gacgcgcctt cgtggcacac agtcatcggc
     1021 atagcaacag taagcaacgg caggtttcca ttgccagttt tctttgacag cagtacttcg
     1081 gcctcgctgg atactgtgat tccagtacca gcgatccttt ctggcttttc cactcaggta
     1141 gcgtttattc agatactggt cgaactcatt cagtatgatg ttcgacagca acggcgagat
     1201 cacaccgccc tgtggcatac tttcactgac cgcccggaag agaccaacat caacacggcg
     1261 ttttccacag cagtctcata aaacgtgtat cactgatcct gcggcagacg gctttcatca
     1321 gcagccggtg atgtactgtg tcgaagtagc tggacaggtc accttcaata acccggcgcc
     1381 ccgtgtttcc ccgcagtcgg tgagctgtaa tagtacgtga tgtctcccgc ccacttctgg
     1441 ttcgggccac tggcgtaaaa atcctgcgtc agcagatttt ctgatacagg caggccgtgt
     1501 gcacggtaac tgatcgggct gaacttctgg gaggcttttg cgctcagttc ctgacggcgc
     1561 agactggctg ccacggtttt tacgttaaag tggtaaccag agcacacagt tcatccgtca
     1621 ggcgtggggc accgtaacgc tggactgggt aaaagccttg cggacaacgc tgtcgcagtg
     1681 ctgacggaac tgcggacgtg tgtttatcct tgtccgccgc tgacaccacg tataccagcc
     1741 g
//
LOCUS       DADLTY010000363         1729 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.369, whole genome shotgun sequence.
ACCESSION   DADLTY010000363 DADLTY010000000
VERSION     DADLTY010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.369"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            114..1718
                     /locus_tag="JN334_004412"
                     /pseudo
     CDS             114..1718
                     /locus_tag="JN334_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT          474 a          395 c          440 g          420 t
ORIGIN      
        1 gacctttctt aaagtactcc aacctactga atcctaaggg atacggcttt aagcctcctt
       61 gcagagttac ttactgtaac ccactgtgta accctctcag ggcatgcaga catatgattc
      121 agctatccag tagtcacaaa cttccagcag tctactatct ctaccagcgt aacggcgtct
      181 actacttccg tcttagggtc agacaatcaa acaatgatag aatgacctca atatcattac
      241 ggaccaagga tagacgcacc gctatggctt actcaaggca cataaaggca gcactcaagg
      301 caatacacgc agacagaccc aacgctacct atgaggaaat gagggaacac cttaaggaca
      361 tcgctgagtg tgaactgtct atgggccgtt cagatctctt tgagcctgac atgagggata
      421 tctaccggga ccaatacgga gaacttggtg agagccttac cgatgctctg gctagtgagc
      481 cgctaagcat tgaccagcat cgttacatca atgaggccct gaaggtgcta aaagcctgta
      541 tgaggcgtat cgaagcggga gacagtcagc ctctgataga ttacgtcgat ctttttaatg
      601 atattgatag gcaagataac caagcagact ctgtgtccct atctgttaat gctccagaag
      661 taaaaccaga agttacgcct agcattacca tagcgagcct ctttgaacaa tacgaagcgg
      721 agaactatca gaactggaag cctgcaacct taagggaaaa caaggcgtca cacgctgcgt
      781 taatcgaaat attcgaccat ctgggtctca atgcagacgc taacagggct gacatgctta
      841 gggtccgtga tgtgctccag cagttaccta ggaaccgtaa gcagcgcttc aaggatgtcc
      901 cacttgctga cctgttgagc cgtgaagata agacagattg tctggatgtc gttaccatca
      961 acaacaagta tcttattaag atggctgctg tctttaggtg ggctgtgcgc aatgacttga
     1021 ttaaaaagaa catgactgag ggtctggagc ttaaagtccc tcagcgtaaa gcctcagggg
     1081 cccgtaacgc cttctctacg gagcaggtgg ggtaactact ggtcgcagct aaggcatact
     1141 ctcagaagac ctctggtaag ccgtatcatt attatgttac cgccttggct gctatcactg
     1201 gggcgagact caatgaaata gcccaactac aggtcaaaga cgtcaggacc actgaggcgg
     1261 gaaccgttta tattcatatc aatgaagacg atagtagtct acccggtaag agcatcaaga
     1321 acgctcatag tgaccgctgt gtgcctctgg tcgatggggc ctatggtttt atattggctg
     1381 actttatggc gcttgtagag acccgtagag gcgctgacgg tgatgacgct atggtgttcg
     1441 atggtctgcg tctcatgaag aacggttacg gtgagcaggt gagtaaatgg tttaatcgga
     1501 cgttgttgcc taaagtcctc gtagaccgta gcggcttagc gtttcactcg tttagacata
     1561 cagttgcggc ccagttaaaa caacatgggg tcgagttggc gtatgcacag gcgatcatgg
     1621 ggcatagttc agggtctatt acgtacgacc gttatgctaa agaggtcgag gttgataggc
     1681 tggtcaatgt gatggctgat gtttataagg agacctagag gagaccata
//
LOCUS       DADLTY010000364         1692 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.370, whole genome shotgun sequence.
ACCESSION   DADLTY010000364 DADLTY010000000
VERSION     DADLTY010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.370"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            420..1493
                     /locus_tag="JN334_004413"
     CDS             420..1493
                     /locus_tag="JN334_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR"
                     /protein_id="HAY7440299.1"
                     /translation="MHTRTKLPAPLAADVAALIKNGMDFLDKAREEFEAKQYKHSVVS
                     FWIAVEILLKVPLASEHWTLVCSGKKVSRKSYLAGDFQSVSFDDVCTRLRDILEKPLP
                     KETEAVFNTIRNHRNRVVHFFHTAFSDSEVETILAEQARAWFALNRLMREDWQQHFAS
                     PHNWALALGETQLLRGNEFYAEARLKHIQPELEQLATEGAEFHPCTICHKPAAIMEIL
                     AVGKNGPTVYEQTCRVCFHSERHVKFTCPECDTDQVLPVEEEDDDTFICRTCNAELSR
                     YNLLDEENFRHVDEMMYPDGLANCAHCEGHETVCVFGENFLCTRCLEIHTGYDTCEIC
                     GTPCEAMGETMRYTGCPHCADED"
BASE COUNT          437 a          407 c          418 g          430 t
ORIGIN      
        1 ttgtgccagg agcggacgtt aagggtttcc aagggagcta tctaatatgg catccacggt
       61 gaacgcattg ccagccggga aatgatgcga ataactgtgt ttaattttat cgagtgagat
      121 tacaatcggt ggcgaaatca ctgtgctgtg gcggactcag ctcggaattt attgaaacta
      181 taacctcgct agggtcttgc tcacattggg taggatcagt cttgtaatgc acctgttgta
      241 tggccttgtt catccgaaga tcctgcaatg gctgatggtg catgagaagt ttgagtgcaa
      301 tgaggtcaac accaaagtct ttgccctcga tgtggtggcc agccagcaac taagatttat
      361 cctctatacc cactaatctc tgcagcagct gccggccccc cgtgataacg gagatcagga
      421 tgcatacacg aacaaaatta cctgcccctc tcgcagctga tgtggcggct ctcattaaga
      481 atggcatgga ttttctggat aaggcacgtg aagagttcga ggccaaacag tacaaacact
      541 cggtagttag tttctggata gctgtagaaa ttttgctcaa ggtacccctt gccagcgaac
      601 actggacgct ggtctgcagc ggtaaaaaag tatcgcgtaa aagctacctt gccggtgatt
      661 ttcagtcggt gtcctttgat gatgtctgta cccggttgag ggatatcctt gaaaagcccc
      721 tgcctaaaga aaccgaagct gtgtttaata ccattcgcaa tcaccgaaac cgtgtagttc
      781 atttctttca tacagctttt agtgattctg aggtggagac cattctggct gaacaggctc
      841 gggcatggtt tgcactgaac cgactaatgc gtgaagactg gcaacagcat tttgcttcac
      901 cacacaactg ggcactggca ttaggtgaaa cacaactcct gcgaggcaac gagttttatg
      961 ccgaagcccg tctgaaacat atccagcccg agctggaaca acttgcgact gagggcgctg
     1021 aatttcatcc ctgcaccatt tgccacaagc cagcagccat catggaaatt cttgccgtcg
     1081 ggaagaacgg tcctaccgtt tatgagcaaa cttgtcgcgt ctgttttcac tcagagcgcc
     1141 atgtaaagtt tacctgtcct gagtgtgata cagaccaggt tctgccggtg gaagaagaag
     1201 atgacgatac cttcatctgc cgcacctgta acgcagaact ctcaaggtat aacttgcttg
     1261 atgaggagaa ttttcgtcat gtggacgaaa tgatgtatcc cgatggccta gcaaattgcg
     1321 ctcactgtga gggccatgaa acggtctgtg tgtttggcga gaacttcctg tgcacacgat
     1381 gtcttgagat ccatacaggc tatgatactt gtgaaatttg tgggactccc tgtgaagcaa
     1441 tgggcgagac aatgaggtat acgggctgcc cacattgtgc ggatgaagac tgaatcgcca
     1501 caaattcagt agccttcttt tagccttcaa cgtctgctcc tcgctcttag cggacattag
     1561 cataggctat ttaccataac gcctcattac gcgtaccgct cagacggcct gagcgcgttt
     1621 ctgacgtatc cccgttaaaa acagtaacaa accacccgaa aatgcacgcc agaaacgcta
     1681 cgtaagaatc ta
//
LOCUS       DADLTY010000365         1655 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.371, whole genome shotgun sequence.
ACCESSION   DADLTY010000365 DADLTY010000000
VERSION     DADLTY010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.371"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(156..1391)
                     /locus_tag="JN334_004414"
     CDS             complement(156..1391)
                     /locus_tag="JN334_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY7440300.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="JN334_004415"
     tRNA            complement(1538..1614)
                     /locus_tag="JN334_004415"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
BASE COUNT          420 a          450 c          394 g          391 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtc
//
LOCUS       DADLTY010000366         1645 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.372, whole genome shotgun sequence.
ACCESSION   DADLTY010000366 DADLTY010000000
VERSION     DADLTY010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.372"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            347..1302
                     /locus_tag="JN334_004416"
                     /pseudo
     CDS             347..1302
                     /locus_tag="JN334_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            1319..1600
                     /locus_tag="JN334_004417"
                     /pseudo
     CDS             1319..1600
                     /locus_tag="JN334_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          462 a          378 c          392 g          413 t
ORIGIN      
        1 cagcattata tagttctact ctggcgagat acgttggcat attcatttcc ttactggttg
       61 tgtgagaact tcagtaaaga taccaccaaa gcccggaagt ggtgaaataa aaccgggcac
      121 aacacgaagg cgcatttccg gtatccataa agagtcggtc ttgtctgtga aatttaaatg
      181 gtgggagtgc gcttccggtt gtaaataacg acattgctgt gtgaagtacc agttggcggc
      241 atcggtttaa ttgctggctg atgtccgccc tttttaaagt gaattttgtg atgcggtgaa
      301 tgcggctatg cgcacgcggc acagttaaaa gtcatgttag tccttattgg tttgggtggg
      361 aaagccaact gtaattgtta actggttaac gtcacctgga ggcaccaggc accgcatcaa
      421 caaagttcac ttcggtgatg aaaggaaaga gaaaatgttg aatgtagcta ttgaaaacca
      481 gaacgggtgg aattatagtg cacctgcacc tcataaaacg ggtgccggga ttgctacccc
      541 gacgatgact actgcgcata accgcgctca ggcggttttt tatgcgtcaa gcacagccac
      601 attcagatta tggtggggcg tgcagggcag ccgcaaggct ggccggtttc ggtagtcacc
      661 ggttgtagca accctgtgcg tctcaccacc catgagattg ctacctctgg tggtgagtca
      721 tttaaactga ctatcgaggc tgccatcatg gctactatcc ttaccctttc tcaccctgac
      781 gcaaccatcg aaaatggtcg cgctgtcact acgtctgttg cggttgcgga gttcttccgg
      841 aaaatgcaca aaaacgttat tcagaagatc gaaactctcg aatgctcgcc ggagtttaac
      901 cggcttaatt ttaagcccgt cacctatacc gacgccaaag gcgaaaaacg cccaatgtac
      961 caaatcacca aaaacggctt cgttttcctg gtgatgggct tcactggcaa aaaagccgcc
     1021 gcattcaagg aagcctacat tgctgagttc gaccgcatgg aggccgaact gcgccagaat
     1081 aacaccccac ctgcagacaa gatgattccg ggtgatggtc gcactctggt tgttcacttc
     1141 gacaaattcg gcaatgtcga attcaccgaa accgttcctg atggcgcact ggtctgtacc
     1201 ctggagactt tccggtttta tctggaaaaa cagggatgga ctcttgttaa ccggggcgca
     1261 attaaaaata tgactgtgga acaattactt tctttaaaat aaaaatggag ctacggatat
     1321 gaaccctatg cttgattatt gttttacctc tgcacctcag cagaccataa cacacaccgg
     1381 aatcaaaaat ttgttatgga taatcaaaaa aaacctccag tagaaaggtg atcccgattt
     1441 aattccctgt gcttatggcc tgtgtgacag acaagtaagc cctgataagg cttacactgc
     1501 tgaacttatt tacatgcacg gaaccatgat gaggaaaaag aaacgcaaat attgtagtga
     1561 aatctgcgcc gaaaaagacc agatggcaca cgaactttaa ttaactgact attcgaaact
     1621 gaatttatgc cagcaatggc aggga
//
LOCUS       DADLTY010000367         1601 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.373, whole genome shotgun sequence.
ACCESSION   DADLTY010000367 DADLTY010000000
VERSION     DADLTY010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.373"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(333..479)
                     /locus_tag="JN334_004418"
                     /pseudo
     CDS             complement(333..479)
                     /locus_tag="JN334_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            533..715
                     /locus_tag="JN334_004419"
     CDS             533..715
                     /locus_tag="JN334_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HAY7440301.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(816..1145)
                     /locus_tag="JN334_004420"
     CDS             complement(816..1145)
                     /locus_tag="JN334_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HAY7440302.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1317..>1601)
                     /locus_tag="JN334_004421"
     CDS             complement(1317..>1601)
                     /locus_tag="JN334_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440303.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          434 a          368 c          378 g          421 t
ORIGIN      
        1 agcaatgtct gtcgcacaac aaccacaatg tcacaaataa aaaatgagaa taaccagttc
       61 cctgaaggcg acgtagcccg gtacaagagt aacccacgtt agcgcttggc gacggcggaa
      121 caaagctgat tcgcgacgta agaaagtggc aggctcatat gacggacctc tgtaatgcgg
      181 tatccaaccc gcgaatatca gcatgattca ccgtcggatt tactgctacg tcgccctcag
      241 ggaattaaac ctaaaaagaa aatcgtcaaa attagagatt ccggtttgac acggaaagtc
      301 atatcagcgt caacggagca ccgtattgac gcttatttat tggtgagaac tacgttccat
      361 ggcaggagtt cgtcaacacg gttggagggc cattcaggca gtatgctcag aatatggcgc
      421 agatacgctt ccggatcgat accgttcaga cggcaggtgc ctgcttttcc ggcaacaagc
      481 aggaatctca gcgctgaagc aggaattaga ggtaaaaacg ccctaccggg ccatgaatca
      541 cccggtcatt ggagtagtaa ccaaagcaga tttagccagc atggaacaaa tatctttggt
      601 gaaaagttgg ttacgggaag ctggagcgca caacgtgtta gtaaccagtg cagttaataa
      661 caatggagtt acagaacttt ttgccttgct gcatacagaa gaaggctgtt gttaatttcg
      721 attggttctc agatgagaat gatgacggtg atacacagat aagaatcaat gagcaatgag
      781 ggttgccggg caaccctcat tgaataaaac gggaattact tcgcttcgcc gtttttcatt
      841 tcgcccatag ctttcagctt tttggagata tcgcggcgtt ctttggagag ctcggcattt
      901 ttgatgatgt aatcgtcaac gcgatcttca tagtcacctt tcatgctggc gatgatgccc
      961 tggattgctt caacgctcat ccctggcttg atgtaatcgc tcaggttgtc cagcaacagg
     1021 acgcgcttct ggttgtcacg gatctttttc tcaacgtcct gaatttcacg ttgcagtttg
     1081 ttcttacgac ggaacagacg aacaaattcc agtacgtcct ggaatgaagg cttggtagtt
     1141 tccattttta tacccctgat aatgtgagag tcggattcgt ttaagcaacc gctattcgtt
     1201 agggccaaca ttactcatag ctgccgcaga tgacaatgct tttatccctt ttcgctatca
     1261 taccctttat ccttgctgaa tcgaagcagc agcaagatga ttctgaagtt caggaattat
     1321 ttgcgcaagg cccggcaaat cagattcgat agcaactcaa gctgatgtgc cgtttccatg
     1381 ttcagccaca cataaccata gattggtgtt tccacgactt tcaggtcatg gtggttatcg
     1441 agcaactgac gcagctcttc cacagcatcg ttcaattttt ccgtattggc aaaaaccggc
     1501 tgcggattac cttcgtacag cgcatgaaca aggctcagca gtatttgctg catcatgtgc
     1561 tgggtatcac aaagtttttg cgcgttcaat aacacgaaat g
//
LOCUS       DADLTY010000368         1597 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.374, whole genome shotgun sequence.
ACCESSION   DADLTY010000368 DADLTY010000000
VERSION     DADLTY010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.374"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(1..702)
                     /locus_tag="JN334_004422"
                     /pseudo
     CDS             complement(1..702)
                     /locus_tag="JN334_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(750..1322)
                     /locus_tag="JN334_004423"
     CDS             complement(750..1322)
                     /locus_tag="JN334_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HAY7440304.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          474 a          337 c          319 g          467 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtgtttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca
     1501 agtttgttat cagacattaa tgataattca gaatatattt atatagaaag atagatgaat
     1561 ttattgatgt cagttataaa aaagggccac cggtcgg
//
LOCUS       DADLTY010000369         1596 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.375, whole genome shotgun sequence.
ACCESSION   DADLTY010000369 DADLTY010000000
VERSION     DADLTY010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.375"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            79..351
                     /locus_tag="JN334_004424"
     CDS             79..351
                     /locus_tag="JN334_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY7440305.1"
                     /translation="MVRKFDELEAKCAAQENKVIPVSTELPPANESVLLFDANGEGWL
                     IGWRSLWYTWGQKETGEWQWTFQVGDLENVNITHWAVMPKAPDAGA"
     gene            351..656
                     /locus_tag="JN334_004425"
     CDS             351..656
                     /locus_tag="JN334_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440306.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            656..1006
                     /locus_tag="JN334_004426"
     CDS             656..1006
                     /locus_tag="JN334_004426"
                     /inference="COORDINATES: protein motif:HMM:NF024139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7440307.1"
                     /translation="MEPYSLTLDEACHFFKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQACIAALQSPLHTVQVSAGDGITEERKCHSSAEVKYGTPVSHCRTVKDLNSLL
                     EQRTKGRRQNSMTS"
     gene            883..>1596
                     /locus_tag="JN334_004427"
     CDS             883..>1596
                     /locus_tag="JN334_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7440308.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGIQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAESCRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQINMQRRVAWINP"
BASE COUNT          529 a          347 c          388 g          332 t
ORIGIN      
        1 cagcctgagt tcacagataa aacactctcc aggaaacccg gggcggttca gaaagtaaat
       61 gaaacagatg ctgaatacct ggtgcgtaaa ttcgatgagc ttgaagctaa atgtgcagca
      121 caggaaaaca aagtaatacc agtgtcaact gaactgccac cagcaaatga aagtgttttg
      181 ttattcgatg ctaacggaga aggctggcta attggctggc gttctctctg gtacacctgg
      241 ggacaaaaag aaaccggaga atggcagtgg acatttcagg tcggggacct tgaaaacgtc
      301 aatatcactc actgggcagt aatgccaaaa gcaccggatg ctggagcata atgaccactt
      361 ttaccgacaa agaactgatt aaagaaatta aagagcgtat cagcagcctt gacgtgcgag
      421 acgatattga gcgccgtgct tatgaaatcg cactcctatc tctggaagta gaaccagatg
      481 aacgcgaagc ttatgaatta ttcatggaaa agcgttttgg tgacttagta gatcgtcgga
      541 gagcaaaaaa cggcgataac gaatacatgg catgggatat gactctcggt tggatcgtct
      601 ggcagcaacg agctggtatc catttctcaa caatgtcaca gcaagaggtg aaataatgga
      661 gccatacagc ctcacactcg atgaggcctg tcattttttc aagatatcca gaccgactgc
      721 cattaactgg atacgcacag ggcgtcttca ggcaacacgc aaagatccca ctaagaataa
      781 atctccttac ctcacaacac gacaagcctg cattgcggct cttcagtctc cgctgcatac
      841 tgtccaggtg agcgcgggtg atggcataac agaggaaaga aaatgtcact cttccgcaga
      901 ggtgaaatat ggtacgccag tttcacattg ccgaacggta aaagatttaa acagtctctt
      961 ggaacaaagg acaaaaggca ggcgacagaa ctccatgaca agctaaaggc tgaagcatgg
     1021 cgggtcagca aacttggtga aatacctgat ataacgttcg aggaagcgtg tgtcaggtgg
     1081 cttgaagaga aagcacataa aaaatcactg gacgatgaca aaagccggat cggattctgg
     1141 cttcaacatt tcgcaggaat acaactaaga gacattactg aatcaaaaat ttattcagca
     1201 atgcagaaaa tgacgaaccg gcgtcatgag gaaaactgga aactcagggc agaatcatgc
     1261 agaaaaaaag ggaaacctgt tccagaatac acgccaaaac cagcgtccgt tgcaacgaag
     1321 gctacgcatc tttcatttat aaaggcccta ctaagagccg cagagcgtga atggaaaatg
     1381 ctggataagg caccaattat taaagtgcct caaccaaaga ataaacggat ccgctggctg
     1441 gagccccatg aagcacaaag gctgattgat gaatgtccgg agccattaaa gtctgttgtt
     1501 gaatttgcac tggcaacagg cttaagacgc tcgaacatca tcaaccttga atggcaacaa
     1561 ataaatatgc agcgccgggt ggcatggata aacccg
//
LOCUS       DADLTY010000370         1569 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.376, whole genome shotgun sequence.
ACCESSION   DADLTY010000370 DADLTY010000000
VERSION     DADLTY010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.376"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..165)
                     /locus_tag="JN334_004428"
     CDS             complement(<1..165)
                     /locus_tag="JN334_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY7440309.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRS"
     gene            164..1303
                     /gene="queG"
                     /locus_tag="JN334_004429"
     CDS             164..1303
                     /gene="queG"
                     /locus_tag="JN334_004429"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAY7440310.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(1286..1339)
                     /locus_tag="JN334_004430"
     CDS             complement(1286..1339)
                     /locus_tag="JN334_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440311.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT          405 a          388 c          402 g          374 t
ORIGIN      
        1 cgaacgacac acctggaatg cggcctcgcc agcgcgaagc atcagctcat agagtgtgag
       61 ccccagcaca tctgccgcct cgcgttctcc gcggcggata tcgtcggcgt accagacggt
      121 gtgtggtata cttacggggt ttttcttcat tgtatggtcc gtcatgtcag agcccctcga
      181 tctcaatcag ttagcgcaaa aaattaaaca gtgggggctg gaactgggct ttcagcaggt
      241 aggtattacc gataccgatc tcagcgagtc cgagcccaaa ctgcaagcat ggctggacaa
      301 acaataccac ggcgaaatgg actggatggc acgtcacggt atgctgcgcg ctcgccccca
      361 tgagttatta cccggtacgc tgcgtgtaat cagcgtgcgg atgaattacc ttcctgctaa
      421 cgccgcattt gccagcacgc tgaaaaaccc caaactcggc tatgttagcc gttatgcgct
      481 gggccgtgac tatcacaaac ttctgcgcaa ccgactcaaa aagctgggcg agatgattca
      541 gcaacattgt gtttcgctga attttagacc gtttgtcgat tctgcgccta ttctcgagcg
      601 cccgttagct gaaaaagctg ggctcggctg gacaggtaag cactcactta tcctcaatcg
      661 cgaggccggt tcgttcttct ttttaggcga attgctggtc gatattccgc tgcccgtgga
      721 tcaaccagtc gaggaaggat gcggcaaatg cgtggcctgt atgacgattt gcccgaccgg
      781 tgccatcgtc gagccatata ccgtcgatgc tcgccgctgt atctcttatc tcaccatcga
      841 acttgaaggg gcgctcccgg aagagttgcg accgttaatg ggaaaccgta tttacggttg
      901 cgatgactgc cagcttatct gcccgtggaa tcgctattcg caactcacta cagaagacga
      961 tttcagcccg cgtaagccgc tacacgcacc ggaactcatt gagttatttg cctggagcga
     1021 agagaagttt ttaaaagtca cggaaggatc ggcgattcgc cgtataggtc acctgcgctg
     1081 gctgcgtaat atcgccgtgg cattaggcaa tgccccttgg gatgaaacga ttttgacagc
     1141 gctggaaagt cgtaaaggtg agcacccact tctcgatgag cacatagcgt gggcgattgc
     1201 gcagcaaata gagagacgaa atgcgtgcat agtcgaagtg caattgccga aaaaacagcg
     1261 tctggttcgg gtgattgaaa aagggttacc gcgtgacgcc tgattcatcc acagcttgtg
     1321 aataaaaata aaaacgcatt gcaattcaag acgaataaaa tcgtcaagtg atcgactcga
     1381 caaaacggaa tcttgatttt taacttattt atcaataggt taaaaaaata ccattcaccg
     1441 tgcgaagttt ctttgaaatt aagattacag cctgaggctg tggataactc tgtttacaag
     1501 agtttgtcag agacaacaaa aaccagcaat aacgcatcgc gtcgctgtag atattttatt
     1561 gagagaaga
//
LOCUS       DADLTY010000371         1492 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.377, whole genome shotgun sequence.
ACCESSION   DADLTY010000371 DADLTY010000000
VERSION     DADLTY010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.377"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            624..1223
                     /locus_tag="JN334_004431"
     CDS             624..1223
                     /locus_tag="JN334_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAY7440312.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
BASE COUNT          394 a          348 c          340 g          410 t
ORIGIN      
        1 tggcttcacc cgtgttaata tcctggataa ctgtgtttga aggatttctg ttaataatct
       61 gctcctgcaa ctcacctgat gagtattagt ggtaatacat aatcttactc gtaaaagagt
      121 ataccttatg tcattaaaca gtcttccggc gtgttgttca atcctgtacc tctgttactt
      181 atctggtacg ttgatatgtg taatgctacg ttaattgcaa tatctggtta tggctttata
      241 ccttcagcat ttaaaatatt ttattaccgg agaaacaggg aagtggaaag actttcggga
      301 taatgtcagg ccattctgac gttaatcaca gtaccaaata acgagagggt aatggaagtt
      361 aaaacctgcg taagttcctc tgttgttcac tggaaaaagt gattgcccac acgcgttata
      421 aaggcctttc cgctgcagcc tggttaacag agcttcaggc gttatatgcg ctgtatggct
      481 gttacgtatg ctgttctttg cctggttctg atacccgtgg aagtcgttac cggaggaaac
      541 acgttcaccc tactacctga gaacatcttc ttcaaaaatt acggcaacgc ttcttcctgt
      601 gttacagaac acgaggaaga aacatgcaaa cttataacac caatcccgct tacgaaatgg
      661 ctccccagct cctggagcac tttaaccagc atctggattc attattcggg gtctattcga
      721 aactgttacc gttcagaatg gattttgctt accgaaaaaa caccctcagc tacagatgtg
      781 cctgcaggta cgccatgtgc gcggagatgc tgcaactgat taacgaggtg ggagagaaac
      841 tggtcgggta tgcctgggtg atggaatata cagaacgtaa agggcttcac attcattttg
      901 tgggctatct gaacggccag agccacagga gttcgtatct ggtatccaga ttgatgggag
      961 atatctggag gcgggttacc gagggtaatg gttactacca ctggtgccgg tttaataaaa
     1021 attatccggt aaacattaac cacgtcattc actattcaga tcacaaagcg gtgaatgatt
     1081 tacgctatgc catcagctac ctggccaaac gggaacagaa agaatgtggc atcatcctga
     1141 aatgcagcgg gcttcctgaa aaaagtaacc ggggtcgacc acgactggac agccccctgc
     1201 cggggatatg tgctctggtg taaacccctg aacgacaagt acgggctggc gctaatcttt
     1261 gctgtcggtt ccggcggcca cttcagcggc actgcgcatt ttttacggcg tggtcattcc
     1321 gtatgaacca tacggagatt taaccatgac acattcttta ccattccttc cggcaaccga
     1381 tacagcacat cccccaatct ccatctctgc ttactgactg ttttttacgt cagtgggcca
     1441 ttgtgtgaca accacagtgc tgtaacagca accagcctgc tttacccaaa ct
//
LOCUS       DADLTY010000372         1470 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.378, whole genome shotgun sequence.
ACCESSION   DADLTY010000372 DADLTY010000000
VERSION     DADLTY010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.378"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(5..256)
                     /locus_tag="JN334_004432"
     CDS             complement(5..256)
                     /locus_tag="JN334_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7440313.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGTFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
     gene            708..992
                     /locus_tag="JN334_004433"
     CDS             708..992
                     /locus_tag="JN334_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HAY7440314.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            992..1267
                     /locus_tag="JN334_004434"
     CDS             992..1267
                     /locus_tag="JN334_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY7440315.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
     gene            1320..>1470
                     /locus_tag="JN334_004435"
     CDS             1320..>1470
                     /locus_tag="JN334_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440316.1"
                     /translation="MLSGQIFCIPLNNLVGDKINYDEITKITARDWRQYRAPGWQITH
                     QKRYCQ"
BASE COUNT          401 a          368 c          361 g          340 t
ORIGIN      
        1 aaaattactc tttttcgaac tccagtgagc gttcacgact catcagtgtg aaaatgatgg
       61 caaaaacagc agacagtatc agtacgataa atgtggcccc ccatcccaga taatcgacaa
      121 caagtcccat tcccagtgtc gcaacaattg agccgaagat ataggaaaat gtgcctgaca
      181 gccctaacag aataagttta cgcgtaaatc gccctgaatc cagaagagca ttaagtatca
      241 cataaacagg ttccatacca gtcctgaccg catcaggacg ccagtaaata aaatctcgta
      301 gtccaccggc atcagagaca agagtaatcg tcgttaccgg tatttcgact ggtttgtact
      361 ggtgtgagcg gacaaaacta ctgtacgtct ctcatacacg ctcgtaagcg gccctcacgg
      421 cccgctaacg cggagatacg ccccgactgc gggtaaaccc ttgtcgggac cactccgacc
      481 gcgcacagag gcattttcat cgctgacagg ccgcatggcc tcgcagagcc ggagggggac
      541 tggtgatatt cggacgaccg taccggcttg cgccgggctt cggcggtttt acacctgtgc
      601 tacatgtaac caccgtatgc cgccttatat gcgctgactc gcattatttg ccttgttccg
      661 tctgaaagta atcactatga ttaaatatga ttaacaacta atcgggtatg caaatgaaaa
      721 acaataccgc acaagcaaca aaagtaatta ccgcgcatgt gccattacct atggctgata
      781 aagtcgacca gatggccgcc agactggaac gctcccgggg ctgggttatc aaacaggcgc
      841 tttctgcatg gcttgcccaa gaggaggagc gtaatcgcct gacgctggaa gccctggacg
      901 atgtgacatc cggacaggtt atcgaccatc aggctgtaca gtcctgggcg gacagcctca
      961 gtactgacca tctgttaccg gtgccacgct gatggaactg aagtggacca gtaaggcact
     1021 ttctgatttg gcgcggttat atgattttct ggtgctggcc agtaaacctg ctgccgccag
     1081 aacggtgcag tccctgacac aggcgccggt cattctgtta actcatccac gtatgggaga
     1141 acagttgttt cagtttgaac ccagggaggt cagacggatt tttactggcg agtacgaaat
     1201 ccgttacgaa cttactggcc agactattta tgtattgcgc ctgtggcaca cacgagagaa
     1261 caggtagatt gggtgtggag gcaactttaa catagcctgc aggtattaat tctggagcat
     1321 tgttatcagg ccagatattt tgcattccat taaataatct tgtaggagat aaaataaatt
     1381 acgatgaaat aacgaaaatt accgctcgtg actggcggca atatcgcgct ccgggctggc
     1441 aaataaccca tcagaaacgt tattgccaga
//
LOCUS       DADLTY010000373         1444 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.379, whole genome shotgun sequence.
ACCESSION   DADLTY010000373 DADLTY010000000
VERSION     DADLTY010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.379"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..371
                     /locus_tag="JN334_004436"
     CDS             <1..371
                     /locus_tag="JN334_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7440317.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLK
                     GKLTPALLQILIREIKGSSH"
     gene            371..718
                     /gene="tnpB"
                     /locus_tag="JN334_004437"
     CDS             371..718
                     /gene="tnpB"
                     /locus_tag="JN334_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7440318.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            738..>1444
                     /locus_tag="JN334_004438"
     CDS             738..>1444
                     /locus_tag="JN334_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7440319.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQILEAQERISALQEEMAETLGEQYDPVLPS
                     PLRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVAGKYADYLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVA"
BASE COUNT          342 a          400 c          399 g          303 t
ORIGIN      
        1 ggcctaattt tccttatgaa tttaaaatcg ctttagtgga gcagtcactg cagcccggag
       61 cctgtgtggc gcagatcgcc cgtgaaaacg gaatcaacga taacctgctc ttcaactggc
      121 gccatcaata ccggaaaggt ggcctgctgc cttccggaaa aaatatgcct gcactgcttc
      181 ccgtgacgtt aacgccggag ccggataata aaatcccggc tccggcacag gaaccagagc
      241 agataaatac accgtccgac agtctgtgct gtgagctggt tctgccggcc ggaactctca
      301 ggcttaaagg taaactgacg ccggcgttat tacagatact tatccgcgaa ataaaaggga
      361 gcagccactg atgatatctt tccctgcagg ttcgcgtatc tggctggttg caggtatcac
      421 cgatatgcga aatggcttta acggcctggt ctcaaaagtt cagaacgtcc tgaaggatga
      481 cccgttctcc gggcatctgt tcatcttccg cggacgccgg ggtgaccaga taaaagtgtt
      541 gtgggctgac agtgacggac tgtgcctctt taccagacgc ctggagcggg gccgcttcgt
      601 ctggccggtc acccgtgacg gcaaggtgca ccttactccg gcccagttat ccatgcttct
      661 tgagggcatc gactggaaac acccgaaacg aacggaacgc gctggcatcc gcatataacc
      721 cgttgtaaag tgaggatatg gacacctcac ttgctcatga gaatgcccgc ctgcgggcac
      781 tgttgcagac gcaacaggac accatccgcc agatggcgaa atacaaccgc ctgctctcac
      841 agcgggtggc ggcttatgct tccgaaatca accggctgaa ggcactggtt gcgaaactgc
      901 agcgcatgca gttcggtaaa agctcagaaa aacttcgcgc aaaaaccgaa cggcagatac
      961 tggaagcaca ggagcgaatc agcgcacttc aggaagaaat ggcggaaacg ctgggtgagc
     1021 aatatgaccc ggtactgcca tctcccctgc gccagtcttc agcccgtaaa ccgttacctg
     1081 cctcacttcc ccgtgaaacc cgggtcatcc gtccggaaga ggaatgctgc ccggcctgtg
     1141 gtggcgaact cagttctctg ggatgtgatg tgtcagagca gctggagctt atcagcagcg
     1201 cctttaaggt tatcgaaaca caacgtccga aactggcctg ttgccggtgt gaccatatcg
     1261 tgcaggcacc ggtgccttca aaacccattg cacgcagtta tgccggagcg gggcttctgg
     1321 cccatgttgt tgctggaaaa tatgcagact atctgccgtt ataccgccag tcagaaatat
     1381 accgtcgtca gggcgtggag ctgagccgtg ccacactggg gcgctggaca ggtgctgttg
     1441 ctga
//
LOCUS       DADLTY010000374         1443 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.380, whole genome shotgun sequence.
ACCESSION   DADLTY010000374 DADLTY010000000
VERSION     DADLTY010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.380"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            82..1275
                     /locus_tag="JN334_004439"
     CDS             82..1275
                     /locus_tag="JN334_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HAY7440320.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1295..1443)
                     /locus_tag="JN334_004440"
                     /pseudo
     CDS             complement(1295..1443)
                     /locus_tag="JN334_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          422 a          313 c          339 g          369 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt ggtcttcggg ccagcaaaag tcgttcaaca
     1021 ggaagaattc tggttctgag tctgctggca acgttgagca ctatcgtaat gtggttactc
     1081 ggttatcacg ctgaaaataa agggttacac ctgaaatatc aggctaacag cattaaatcc
     1141 cggcgggtta tctcatatct gacgttagcg aagaatgttc ttcgacactc gccacttatt
     1201 ttaagacgaa cagtactgag cacagttcta aaccatctgt ccagaaccta ccgaaatatg
     1261 gtgctggttt attagcagta attttgtggg gatccctcag cctgaagggg cgacacagtc
     1321 ggggagagaa gcccatctcc ttgccgaact gccacagcga aggatggaac cggtgctgac
     1381 cggtctgata tgcgtcacgt tgcagaacca cagttttcat attgtcatac aacacttcgc
     1441 gcg
//
LOCUS       DADLTY010000375         1433 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.381, whole genome shotgun sequence.
ACCESSION   DADLTY010000375 DADLTY010000000
VERSION     DADLTY010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.381"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            1..252
                     /locus_tag="JN334_004441"
                     /pseudo
     CDS             1..252
                     /locus_tag="JN334_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            745..>1433
                     /locus_tag="JN334_004442"
     CDS             745..>1433
                     /locus_tag="JN334_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7440321.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          347 a          331 c          445 g          310 t
ORIGIN      
        1 gccaacacaa aggcaactaa taaagataca gataaagatc taaaaacaga tctaacccat
       61 cccaaaccct tcccttccgg aagggagttt cgggattttg tggctggagt gcttgagggg
      121 agattatctg gcggtactgc agcggaattt tgtaattctg cggtggttgc gttgcaggct
      181 gctggcctgg atgtctgtcg tgagtatccg gtgccagagc gtggtgacgg ttgcggaggg
      241 cggattgata tcgtcgtgac tgacaggaac ggtgtccggt gtgggatcga gcttgaccga
      301 aattctccgc gacagaaatc actgctcaaa atcggtgctg ttgaaaccgg gatatgtgtc
      361 ttgcggcgca gtgatatcgc aaggcacacc gagcagggaa ttctggttat cggtggggct
      421 gttcgccaga aaaaatttga cccgttgtca gttgatctgc ccgactggtt gtcagaaaca
      481 ctctggcatg agtgggtcca gttcaggcag gcattgcgaa aaccgattcg aacggagctg
      541 ggcgctaacg gggcgatacg ggaactggaa aaattccgtc agcagggttt tacacctgag
      601 caggtgattc gacacagcat cgccaatgaa taccagggtc tgttcgcgcc gaaaggtgtt
      661 cggcctgaga cgttgctccg acaggttaac accgtctcgt tgccggacag tgcgatcccg
      721 ccaggcttca gggggtaaca gaccatgaaa aatattgcga caggaggcgt tctggagcgt
      781 atccgcagac tgaccccacc acatgtaacc gccccattca gaacggttgc ggagtggcgc
      841 gagtggcaac ttgctgaagg ccagaaacgt tgcgaggaga tcaaccgcct gaatcgtcag
      901 ttgcgggtgg aaaaaatcct gaaccgctcc ggcatccagc cgttgcaccg gaagtgttcg
      961 tttgcgaatt accgggcgca gaacgacggt cagcgacatg cactgagtca ggcgaaatcc
     1021 atagctgacg aactgatgac aggctgtacg aattttgtgt tcagcggtaa gcccggcacc
     1081 ggaaaaaatc atcttgcagc cgcgattggc aaccggctga tggcgaaggg gcgtagcgtg
     1141 attatcgtca ccgtgtccga tgtcatgagc gtgttgcatg agagctacga caacggcaaa
     1201 tccggtgaaa aatttttaca ggagctttgt ggtgttgacc tgctggtcct ggatgaaatt
     1261 ggcatgcagc gggatacgaa aaacgagcag gtggtactga accagattgt tgatcgccgg
     1321 acggcatcgt tacgcggtgt ggggatgctg acaaatatta accatgcagc gatgaataca
     1381 cttctcggcg agcgggtgat ggatcgcatg gtcatgaacg gcgggcgctg ggt
//
LOCUS       DADLTY010000376         1404 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.382, whole genome shotgun sequence.
ACCESSION   DADLTY010000376 DADLTY010000000
VERSION     DADLTY010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.382"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..167
                     /locus_tag="JN334_004443"
     CDS             <1..167
                     /locus_tag="JN334_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HAY7440322.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="JN334_004444"
     CDS             152..790
                     /locus_tag="JN334_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAY7440323.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1018..>1404
                     /locus_tag="JN334_004445"
     CDS             1018..>1404
                     /locus_tag="JN334_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440324.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          347 g          393 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg
     1021 aaaaaacaga tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg
     1081 aaaatattta aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc
     1141 gaaccattcc acatatctgt ccaaaatact gttatttatg tatcaaaata caatggtata
     1201 tttattaata aattagttcc tttttctctt ttatttgaca gggatatatc tgttcttcaa
     1261 agaagagata tttgtgttgt gcgatttacg tcagaggaaa taagtgaaca taacgtttta
     1321 tttgatcatg acattgaacg attaaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagt
//
LOCUS       DADLTY010000377         1366 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.383, whole genome shotgun sequence.
ACCESSION   DADLTY010000377 DADLTY010000000
VERSION     DADLTY010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.383"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..292
                     /locus_tag="JN334_004446"
     CDS             <1..292
                     /locus_tag="JN334_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HAY7440325.1"
                     /translation="AYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLFES
                     LCIDHLQCHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            352..>1366
                     /locus_tag="JN334_004447"
     CDS             352..>1366
                     /locus_tag="JN334_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7440326.1"
                     /translation="MGFAGDGKTYTASELAIGLVMLMRQRGIEAGNRPVMFLDTETGS
                     DWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMIIDSISHFWTGLCDEYARR
                     RNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAGYEYDFFEGDDGKRQLAKT
                     GIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKDRSTRIDGQVFSNPTFKNF
                     LPHIESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRAKEIALDEIIELLNKHHGG
                     TSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNALWIELEGVEYAFPDPQTQN
                     ETQQ"
BASE COUNT          422 a          312 c          334 g          298 t
ORIGIN      
        1 cgcttatccg atttttgatc gtattgaaga aatggcatgg gctcgtcact accagcagat
       61 cgttcgtgaa gaaaaagaaa cggaactggc ggacgacctg gaaaaaggtc tgcctcagca
      121 cctgtttgaa tcgctctgca tcgaccattt gcaatgccac ggtgccaata aacaggcaat
      181 cagccacgca tttgatgatg atgttgagtt tcaggagcgc atggcagaac acatccggta
      241 catggctgaa accattgccc accaccaggt tgatattgat ttggaggact aaaatctatg
      301 gcgctttttc aacgagcaat aaacacacag gcctatctaa aagcaggaat tatggggttt
      361 gctggcgacg gcaaaaccta cacagcaagc gaactggcta ttggtctggt aatgcttatg
      421 cggcaacggg gaattgaagc tggtaaccgc ccggtaatgt ttctggacac agaaacaggc
      481 tctgactggg ttaaaccccg ctttgatgcg gaaaatatcg aattatttac ggcaaaaacc
      541 cgctcgtttg ttgatctgct tgaagccatt aatgaagcgg aaagcagcgg ttccgtaatg
      601 atcatcgact ccatcagtca cttctggacc ggattatgcg acgagtatgc cagacgccga
      661 aaccgtaaac gaggactgga attttcagac tgggcgtggc tcaaacagga gtggcgacgc
      721 tttaccgatc gatttgttaa cagtcaggcg cacattatta tgtgcgggcg tgcaggatat
      781 gagtatgact ttttcgaagg tgatgatggc aaacgccagt tggcaaaaac cggtataaaa
      841 atgaaagcag aaacagaaac cggttatgaa ccctccatcc ggatccagat ggagaagcaa
      901 atgaacatag aaaccggtca ggtgtggcgc accgccagaa tactaaaaga tcgctccacc
      961 cgtattgatg gacaggtatt ttcaaaccca acatttaaaa actttcttcc tcatatcgag
     1021 tctcttaacc tgggaggtga acaccccggc atcgatacct cacgggataa cagtgaatta
     1081 tttgccaacg acggtacacc gacatggcta aaagaaaagc gtgcaaaaga aatcgcactt
     1141 gatgaaatta tcgagttgct caacaagcac catgggggca ccagtaacga cgccaaacgc
     1201 gcaaaagctg atttgctgga aaaaacattc acctcccgct catgggaaag aatcaaaggt
     1261 atggactggc cgacaatcaa agctggtcgt aatgcactat ggattgaact ggaaggcgtt
     1321 gaatatgcat tcccggatcc acaaacacaa aacgaaacgc aacaag
//
LOCUS       DADLTY010000378         1356 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.384, whole genome shotgun sequence.
ACCESSION   DADLTY010000378 DADLTY010000000
VERSION     DADLTY010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.384"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(3..485)
                     /locus_tag="JN334_004448"
     CDS             complement(3..485)
                     /locus_tag="JN334_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7440327.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="JN334_004449"
     CDS             complement(476..778)
                     /locus_tag="JN334_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY7440328.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1050..1322
                     /locus_tag="JN334_004450"
     CDS             1050..1322
                     /locus_tag="JN334_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440329.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          398 a          342 c          288 g          328 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgcctg aaccacggca gcctggcggc tgcaaatcgg ggtgtggcag aatatgagct
     1021 ttcagagtaa ccatccagaa tgagttccca tgtcacacaa cctggagcac cagaaagtcc
     1081 acaccagaat ggtgaaagag gttctgaaag ccgtcgccag agccaataat cacccttata
     1141 agtccgtatt tgcagacttt atcaccggtc atccctcctg cacggtctgt ttctgggaaa
     1201 ccttccacaa aatgtacccg gattcaccgt acgaatacgt cacgttctgc catacctgcc
     1261 gtcgttttga tttatacgaa acagaagcgg agatgaaggc ggatgacccg aagtggtggt
     1321 gattttcagc cccgaccgct gaaacgcctg ttcaca
//
LOCUS       DADLTY010000379         1339 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.385, whole genome shotgun sequence.
ACCESSION   DADLTY010000379 DADLTY010000000
VERSION     DADLTY010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.385"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(210..422)
                     /locus_tag="JN334_004451"
     CDS             complement(210..422)
                     /locus_tag="JN334_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7440330.1"
                     /translation="MLCTCRLASYMPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFVDYESRK"
     gene            complement(593..1258)
                     /locus_tag="JN334_004452"
     CDS             complement(593..1258)
                     /locus_tag="JN334_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAY7440331.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          331 a          342 c          316 g          350 t
ORIGIN      
        1 ggtccatccc tcttctccct gcgcgctact taagtgagtc gattctatct gcgaaggtgc
       61 gcgaatcaaa ttcaccggaa ataaacaaca aaaaacccgc cgaaacgctg agagatcaca
      121 gcgatgggcg ggttaagtgc gggtgcgttg aggatgcctg acacatcaga ggtgggcggg
      181 gatgggatca gctccccacc cggtcactct tacttcctgg attcgtagtc tacgaagaca
      241 gcgacctccg tctggccggt tcggattcgt acctcacaga ggtctttcct cgttaccagt
      301 gccgtcacta tgacggttaa acagatgacg atcagggcga ttaacatcgc cttttgctgc
      361 ttcatagcct gcttctcctt gcctttcggc atgtaagagg ctaacctaca tgtgcaaagc
      421 atgaaattgg cctcagatta atgttaagcg tcttgccgga cgcgtaatgt taactggggc
      481 ttttctctat ctgccttttg gtgttcatgc ctgaagcaga tagcctcaag cacccgcagt
      541 cattctactt acctcgccaa tatgaaatca atcagaaagg tgccccataa aatcactcct
      601 tctcttcttt accgtagtgg agttgaccaa ttttgataag agggcgtccc tgagatttgc
      661 ggtgtagatt ggtatcgcgc agagaataca cacagccaca atattcctgc tgatagaatt
      721 tttcgcgctt gctgatttca atcatacggg acgagccgcc ctgcttgcgc cagttataat
      781 cccagtacac catgcccgga taatgcgcga cggctcgccg cccacagtcg ttaacctgct
      841 gcatattttt ccagcgtgaa atgcccagtg aactgctgat cacactgaaa ccattttcag
      901 cggcgtacag cgctgtccgc tcaaaacgca tgtcaaaaca catggtgcaa cggatccctc
      961 gttcgggctc ccattccatt cctttggctc gttcaaacca gttgtctgtg tcgtaatcag
     1021 catcaataaa cggcacgccg tgttgttcag caaagcgaat attctcatcc ttacgaatta
     1081 aatactcttt ctgaggatga atgttcgggt tgtagaaaaa gatggtgtag tcgatttccg
     1141 aggcctgaag cgcctccatc acctcaccgg aacatggagc acagcaagag tgcagtagta
     1201 gtttgtttgc cccgtttggg agctccaatt tgggccgttt gaaatcagca actgtcataa
     1261 atatgtttat tgaggtcata aaaatatcaa agagtgtagc attagaacgg ggctatcgga
     1321 aacagatgtg tgactgctc
//
LOCUS       DADLTY010000380         1313 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.386, whole genome shotgun sequence.
ACCESSION   DADLTY010000380 DADLTY010000000
VERSION     DADLTY010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.386"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(235..924)
                     /locus_tag="JN334_004453"
     CDS             complement(235..924)
                     /locus_tag="JN334_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HAY7440332.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(921..1280)
                     /locus_tag="JN334_004454"
     CDS             complement(921..1280)
                     /locus_tag="JN334_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7440333.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
BASE COUNT          325 a          368 c          265 g          355 t
ORIGIN      
        1 ttgaaccagc gacctggcga ttatgagtcg ctcgctctca ccactgagct aaagggccgg
       61 aggcagaata ataaccatat gtcatcacat ctgcaaactc atctgaccac cagcacgttt
      121 aacgtcctgt gccatttttc aggcataaaa aaaccgctcg gcggcgggtt ttgcttactt
      181 taccatcgcg tacaaaatcg gcaaagtatc agatttgcat gagatatatg ccttttaatc
      241 tacttttgca atactttgct ttgaaaatgc cgctttttgt tctgaacgtg ttctcatcac
      301 aaacaataaa gcctcactat ccagtcggtg aaaaatgtgt ttcattacaa cccagtgacc
      361 agtaaatgtt ttggaccagt ttttggttgt tactcccgcc agtaatgcca gctcctgata
      421 ttcataacct tccccaccaa aaagttctgc ttttaccgcc tgcgccgcca gccagattaa
      481 ttttttcagg cgttcctgtg ttttctctgc aattttccta gtgccagaat gagccttaaa
      541 ctcttcccac gcccagtggg ttatggtgat ctgatactcc cagcaaatac ttccgctgta
      601 acaccacaac aaccaggctt tatagtgctc ttcaagagac agaacagccc gccgccacga
      661 tgatgtcgaa aacgcaacct gactgaccag aggaattgac gcccccttcg ccagtgattg
      721 ttttcccggg attggtggat tatcccgcgt tatcattttt ccggtcacct catcgcggta
      781 ccggattttt ttacgcctgt aacgccctgt atcgaacatg gcattctcct gccaggcttc
      841 aagctgacct tttgtcgccc cactcaaatc agcggtggcg ataatgagct gctcacgaac
      901 aaactgtaaa tactggttat tcatgcacac tccagctctg tgatttttat ccccaaccga
      961 ccaccaggaa caggcagtcc gcgcacaata ttggtttcat caaactgctc gtcgtcgata
     1021 agcaaccccg catgtgtcag tgcatccagt ggtgctttca ggatattgtc caggtcacga
     1081 cgccgcttat ccggtggctc tgcaataatc tttatcgcca gtcttccgga caggcttaat
     1141 ttcagtcgct gctgacggac aataagcgcc acatcccggc gataacgctc accggctttt
     1201 gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat tcaccgtcgg cggataaggt
     1261 aaaataaact catggcgcat cagcgcagca cctcctgcac cagcttttca aac
//
LOCUS       DADLTY010000381         1277 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.387, whole genome shotgun sequence.
ACCESSION   DADLTY010000381 DADLTY010000000
VERSION     DADLTY010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.387"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="JN334_004455"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="JN334_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HAY7440334.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          358 a          229 c          286 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DADLTY010000382         1275 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.388, whole genome shotgun sequence.
ACCESSION   DADLTY010000382 DADLTY010000000
VERSION     DADLTY010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.388"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            309..1058
                     /locus_tag="JN334_004456"
     CDS             309..1058
                     /locus_tag="JN334_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY7440335.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          362 a          255 c          258 g          400 t
ORIGIN      
        1 aagttatcgg tgcgtttatc gaaaaacata tgttctacta attggagtca ttgtctgtaa
       61 aagcaactca aaaatacaag ttgcttattc cccttgttgg taagaactta attctaactg
      121 tttgttctgg gtgttttaaa cgtcctcctt tttatgcttg ttcataacgg atatttaata
      181 ggtaaccttt gttttagcat cttctgattt atttaaatta tgtgatagta cacacaattt
      241 tattatccag tactttatgc tcttaataaa ataattataa atatgtatcc tctataagga
      301 gatgttatat gaaaagacag ctttttactc ttagtattgt cggggtattt tctttaaata
      361 cctttgcatc attccctccg ggaaatgatg tgacaacaaa acctgacctt tactacctga
      421 caaacgataa tgctattgac agtctggcat tattaccgcc tccaccacaa atcggaagta
      481 ttgccttcct gaatgaccag gccatgtatg aaaaggggcg tttattgcga aatactgaaa
      541 gagggaagct ggcagctgag gatgccaatc tcagtagtgg tggcgtggca aatgtatttt
      601 ctgctgcttt tggttctccg ataacggcta aagattcacc agaattgcat aaattactga
      661 caaatatgat tgaggatgcc ggagacctgg caacgcgttc agcaaaagaa tattatatgc
      721 gtattcgacc ttttgctttt tatggtgttt ctacgtgtaa tacaaaagaa caggatacat
      781 tatccagaaa tggctcttat ccatcaggtc atacatcgat tggttgggca acagcacttg
      841 tattgtccga aataaatccg gcacgccagg ataccattct gaaacggggt tatgaactgg
      901 gggacagcag ggttatttgc ggttatcact ggcaaagtga tgttgacgca gcacgtattg
      961 ttggctctgc tattgtagca acactgcact caaatcctgt gttccaggca caattacaaa
     1021 aagcgaaaga tgaattcgct aacaatcaga aaaaataatg cttttagcca gtacgcctta
     1081 taaccccgac cttcagggct aatgccagtc agttaagcaa ctgactggct ctttttcagg
     1141 gctgtggggt atttccagtg cctctccttt accactctcg gaaaggccct ttcccttctt
     1201 gtcggtaatt tcactaggct gtgtccctta attacgtaag cgttgataaa agagtcggat
     1261 gcagcccagt ttcac
//
LOCUS       DADLTY010000383         1232 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.389, whole genome shotgun sequence.
ACCESSION   DADLTY010000383 DADLTY010000000
VERSION     DADLTY010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.389"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JN334_004457"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JN334_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HAY7440336.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          365 a          206 c          293 g          368 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattcctatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gt
//
LOCUS       DADLTY010000384         1159 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.390, whole genome shotgun sequence.
ACCESSION   DADLTY010000384 DADLTY010000000
VERSION     DADLTY010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.390"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..118
                     /gene="relE"
                     /locus_tag="JN334_004458"
     CDS             <1..118
                     /gene="relE"
                     /locus_tag="JN334_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HAY7440337.1"
                     /translation="SGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            190..345
                     /gene="hokD"
                     /locus_tag="JN334_004459"
     CDS             190..345
                     /gene="hokD"
                     /locus_tag="JN334_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HAY7440338.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            563..814
                     /locus_tag="JN334_004460"
     CDS             563..814
                     /locus_tag="JN334_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440339.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            881..1159
                     /locus_tag="JN334_004461"
                     /pseudo
     CDS             881..1159
                     /locus_tag="JN334_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          311 a          271 c          292 g          285 t
ORIGIN      
        1 ttcaggctat cgccttgtat accaggttat agacgagaaa gttgtcgttt tcgtgatttc
       61 tgttgggaaa agagaacgct cggaagtata tagcgaggcg gtcaaacgca ttctctgaac
      121 caaagcatga catctctgtt tcgcaccgaa ggtgacactt ctgctttgcg ttgacaggag
      181 aagcaggcta tgaagcagca aaaggcgatg ttaatcgccc tgatcgtcat ctgtttaacc
      241 gtcatagtga cggcactggt aacgaggaaa gacctctgcg aggtacgaat ccgaaccggc
      301 cagacggagg tcgctgtctt cacagcttac gaacctgagg agtaagagac ccggcgagtg
      361 agaaatccct cgccacctct gatgtggcag gcatcctcaa cgcacccgca cttaacccgc
      421 ttcggcgggt ttttgttttt attttcaacg catttgaagt tctggatggt gccggaatag
      481 aatcaaaaat acttaagtag cgcgcaggga taaaagggat ggaccccgaa caggggagtg
      541 ctacttatct ggaaggattc tgatgatgaa catcgaagaa ctgcgtaaaa ttttttgtga
      601 agatggcctc tatgctgtgt gcgttgaaaa tggaaatatt gttagtcatt accgcattgt
      661 gtgtttgcaa aaaaatgggg ctgcgttaat taattttgtg gatgcccgag tgacggacgg
      721 atttatcttg cgcgacggtg agtttgtcac ttcattacag gcattgaaag agatcggaat
      781 aaaagctggc ttttctgctt tttcagaaga ataaactcat ctacaatctt gcgcggggct
      841 gaactcccgc tgagtaacac cgtgccaccg gagaaaaccg atggcacgca acgcaaaata
      901 ttacaatcat gataattcga ccgttcttgc ccacacgcac gagcggtatt ctcacgcatt
      961 taagtcagac tggtaccagc atccctcatg cactgaagaa caggccgaat ggcttattca
     1021 gtgttaccgc aggcgcggat gcgaggttaa aaaagccctt agcctcgact accgtcacta
     1081 gataatctcc gtcaggctcc cttactccga acagccaccg cgtccgtccc gcacattcca
     1141 gcaacggatc tggaggtaa
//
LOCUS       DADLTY010000385         1159 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.391, whole genome shotgun sequence.
ACCESSION   DADLTY010000385 DADLTY010000000
VERSION     DADLTY010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.391"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            115..>1159
                     /gene="cadB"
                     /locus_tag="JN334_004462"
     CDS             115..>1159
                     /gene="cadB"
                     /locus_tag="JN334_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HAY7440340.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          246 a          255 c          298 g          360 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcaactatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctg
//
LOCUS       DADLTY010000386         1146 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.392, whole genome shotgun sequence.
ACCESSION   DADLTY010000386 DADLTY010000000
VERSION     DADLTY010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.392"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            116..>1146
                     /gene="curA"
                     /locus_tag="JN334_004463"
     CDS             116..>1146
                     /gene="curA"
                     /locus_tag="JN334_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HAY7440341.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAG"
BASE COUNT          273 a          248 c          353 g          272 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtga
//
LOCUS       DADLTY010000387         1144 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.393, whole genome shotgun sequence.
ACCESSION   DADLTY010000387 DADLTY010000000
VERSION     DADLTY010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.393"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..585)
                     /locus_tag="JN334_004464"
     CDS             complement(<1..585)
                     /locus_tag="JN334_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY7440342.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVR"
     gene            complement(582..944)
                     /locus_tag="JN334_004465"
     CDS             complement(582..944)
                     /locus_tag="JN334_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY7440343.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT          335 a          257 c          234 g          318 t
ORIGIN      
        1 gcgtacatat tcaacgacat ccgtcggacc acccacccag ctcagtatgc ctttcatgaa
       61 aagattgcgc tcaggcaaca gtttaatgtt ctccacaacc tcacgagaca tgagtcgaaa
      121 atctccgaca ttttcctcga tctttggggt gcttattttg ttgtgtaatt tatagaacca
      181 ctcagctgtc ttacgtttca gtcgtccatc agttgagcgg tctgaacgtt tagcaagcac
      241 catgtcagca cctgcctgcc acttttcaat aagatgaggg ataacctcaa ttgggtcttg
      301 caggtcgaca tcaataggaa ttacagcatc gccgcttgca tggtctaacc ctgcaaataa
      361 ggctggttct ttaccaaagt tgcgtgtaaa tgacagcgga acaactagcg ggtctgaaac
      421 agccagcgcg ttaataattg actctgtggc atctttactt ccgtcattta tgaatacaat
      481 ttctacttca tatggcttca actcttggaa ttcacgtacc gttttataga aaacaggtat
      541 cgcttcttct tcattgaaga caggaacgac aagagatatt ttcatttcgc atccctaaag
      601 acaatgaact ttgaatagac gaaaccgcac accagactga tggcggagaa ggtgataaga
      661 gtgactattg gaggaagtga gcatttatca gctgcccaac caacaatcac actcaatatt
      721 cccataaatc ccacgtataa catgtagcgc atcgctgtag tcaatgcttt gaatgtgaat
      781 cttgcattcg cgaagaagct aaagctcaca gccactacga aacctgtgaa gtttgccaga
      841 gcctgactgg tatgtgcggc atagatacat acaccaaaaa ccacccagtg tataagtgtg
      901 ttcagcacac ctatagatgt gtactttaca aataacttta acatttattt aatcaatgag
      961 ctctgaaagg catgaagtct atcatccaag tctcaattga tcgatacttg ctatgtctga
     1021 tgagacaaaa ctgagacaca taaggcctca caatggcttg caaggcttta catgttttga
     1081 tgtggtggga cgtgtgagcg cagtgttgat gggataatcc tttgaattac aagcggattc
     1141 ttat
//
LOCUS       DADLTY010000388         1140 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.394, whole genome shotgun sequence.
ACCESSION   DADLTY010000388 DADLTY010000000
VERSION     DADLTY010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.394"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            35..730
                     /locus_tag="JN334_004466"
     CDS             35..730
                     /locus_tag="JN334_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAY7440344.1"
                     /translation="MLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVNKPAPSMPVIHD
                     VPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFTGICYSHRVFVA
                     LNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGVHNHFGVSWFNP
                     EDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRPLPADYVTHCQN
                     GSVKIITPDSEDE"
     gene            complement(732..830)
                     /locus_tag="JN334_004467"
     CDS             complement(732..830)
                     /locus_tag="JN334_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HAY7440345.1"
                     /translation="MISQIDKLEYVVKVQRNQSNPTMFNKIVVFFQ"
BASE COUNT          298 a          283 c          247 g          312 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt
      361 aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag
      421 taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt
      481 gcataatcac tttggcgtca gctggtttaa ccctgaagat cttgccacta agcgtaaagt
      541 gatgaaccgt ggactacgga aaattttgct cgcccgccac gcgttgttcg atgaagtggc
      601 ctctgccagc ctcgcaccga tctctgcatt tgacgttctg attagcgatc gtccgttacc
      661 ggcagattat gttacgcact gccagaatgg ttctgtaaag atcattacac ctgattcaga
      721 agacgaatga cttactgaaa aaacaccaca atcttgttaa acatcgtcgg gttggactga
      781 ttacgttgca ctttcaccac atattccagc ttatctattt ggcttatcac ctactccaga
      841 cgctggtcat ccttgaccag tagccagata tggcttttgt cgcagtcctg aatcggcagg
      901 cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag cccacaaagg tgagtcatta
      961 cacccggatg gtaaacaggc acttccttgt tgtagaagcc gttatattgt ccataatgtc
     1021 tgggttttta atgcatactg ggtaacaacc ggacgaatct tcgaatatca ctggcgctgc
     1081 cagccggttt gaattcatct cacaaccctg catagagcga atctcctgcc tgcacgtcac
//
LOCUS       DADLTY010000389         1109 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.395, whole genome shotgun sequence.
ACCESSION   DADLTY010000389 DADLTY010000000
VERSION     DADLTY010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.395"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            1..1019
                     /locus_tag="JN334_004468"
                     /pseudo
     CDS             1..1019
                     /locus_tag="JN334_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            992..>1109
                     /locus_tag="JN334_004469"
     CDS             992..>1109
                     /locus_tag="JN334_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HAY7440346.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          312 a          271 c          258 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggtta
//
LOCUS       DADLTY010000390         1100 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.396, whole genome shotgun sequence.
ACCESSION   DADLTY010000390 DADLTY010000000
VERSION     DADLTY010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.396"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            55..>1100
                     /locus_tag="JN334_004470"
     CDS             55..>1100
                     /locus_tag="JN334_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HAY7440347.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          344 a          255 c          178 g          323 t
ORIGIN      
        1 cattaggaga gatacattaa atacaattta ttcacaatct aattccatat ccatatgcga
       61 gaaattaata tgctcagaaa catttcatcc tgtttatttc cacatatcag cacaattaca
      121 tcccccaacc attatttgtc cgaatgggat gattgggaga aacaggggtt accggaagaa
      181 cagcgtactg aggcggtaag aagacttcgt gcatgtctta cctctaaggg gcataaactg
      241 gacctgcgag ccttggcgct ttcctcgcta cctgtactcc ctgcttgcat taaaaagctt
      301 gatgtgagct gtaataaatt aaccatcctt actgatttac ctgaaaatat taaagaactt
      361 attgcaagag ataatttctt aacacatata tctgcattac cacattatct aataactttg
      421 gatgtgtccg aaaatcaatt agagaatctg ccgttattac cagacaccat caaatcacta
      481 agcgcagagt ataataggtt atccacactg ccttcattac ccttgaattt aaaaaaactt
      541 gaggttagga acaacgaact gcaaactctt ccatctctgc cttctaatct taagatactt
      601 aaggttgcgc acaaccatct tactgaactg ccccctttac ctaggagact gcaacttctt
      661 tttgcatata gcaatagatt aagcaactta ccaaacatcc aagaaaatat tatcatgaga
      721 agattttttt attttgaaaa caaccaaata actacaatcc cgacaaatct ttttcgttta
      781 gatcctcata taactattga gattgcaaat aaccccttat cagatcaaac tctgctattc
      841 ttaatacagc aaacttcggt tccaaatttt aacgggcctc agtttcgtat ttccctgtca
      901 gaccaaaaca gactgttttt acgccagatg ttgccgcaaa atttacattc gcgccatatc
      961 agagtcatca ctgaaggggg gcagaacttt cagatccccc ctcttcccga aactgtggca
     1021 gcctggtttc ctgaagcaga tcgtcgggag gtttctacac aatggacttc tttttccacc
     1081 gaggagaatt cccgggcatt
//
LOCUS       DADLTY010000391         1077 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.397, whole genome shotgun sequence.
ACCESSION   DADLTY010000391 DADLTY010000000
VERSION     DADLTY010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.397"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(109..1074)
                     /locus_tag="JN334_004471"
     CDS             complement(109..1074)
                     /locus_tag="JN334_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HAY7440348.1"
                     /translation="MTMNLHTQTTAPKASQATSVSPLEQSGSSKTKFSRGKSKARAGA
                     VKTDLYQTVTDSIIAALESGVKPWVCPWVRNGAAVGLPANFSTGTAYSGINIMLLWCS
                     AAKQGFQDERWLTYKQAQELGGQVRKGEHGTTAIFYKTLEKEDEDGEIEKIPMLKAFT
                     VFNVEQIDGLSIESVPQPVAGFDPLPQAEALMIRSGAKITEQGVKAFYRPATDEIVLP
                     ERFRFADAANFYATGLHELVHWTGAASRLNREKGNKFGSEAYAFEELIAELGSAFLLA
                     DLGITGEVQHESYIASWLKALKGDKRYIFKAAAAASKAHRWLMDC"
BASE COUNT          200 a          336 c          278 g          263 t
ORIGIN      
        1 gaggccggcc gcgttacaga attctaaaaa acagtgactt atataattcc ctccctgatt
       61 ctctctcttt caaaaattct gaagccctgc accgcagggc tccagctgtc agcagtccat
      121 cagccagcgg tgcgcttttg aggccgctgc cgccgcttta aagatgtagc gcttgtctcc
      181 cttcagcgct ttcagccagg acgcgatata gctttcatgc tgcacctctc ccgtaatccc
      241 caaatccgcc aacagaaacg cgctgcccag ctcggcgatc agttcctcaa acgcatacgc
      301 ctcagagcca aacttattgc ctttttcacg attgaggcgg gacgctgcac ccgtccagtg
      361 aaccagctca tgcaggccgg tggcgtagaa gttcgccgcg tccgcgaagc ggaaccgctc
      421 cggcaggacg atttcatcgg tggccggacg atagaacgcc tttacgccct gctcggtgat
      481 ttttgccccg ctccggatca tgagtgcttc cgcctgcggc agcggatcga aaccggcaac
      541 cggctgcggc acgctctcga tgctcaggcc gtcgatttgc tccacgttaa acacggtaaa
      601 ggctttcagc atcgggattt tttcgatttc cccgtcctcg tcctctttct ccagcgtctt
      661 gtaaaagatg gccgtcgtgc cgtgttcacc tttacgaacc tggccgccca gctcctgcgc
      721 ctgtttgtag gtcagccagc gctcgtcctg aaaaccctgt tttgccgcac tacaccacag
      781 cagcatgata ttaattccgc tgtatgccgt gccggtgctg aagttggccg gtaatcccac
      841 cgctgcgccg ttgcgaaccc acggacacac ccacggctta acgccgcttt ccagggctgc
      901 aatgatgctg tccgttactg tctggtaaag gtctgttttg actgcgccag cacgggcttt
      961 gcttttgccc ctggaaaatt tcgtttttga ggagcctgac tgctccagcg gggatacgct
     1021 ggtcgcctgt gaagctttag gggcggttgt ctgagtgtgc aggttcatcg tcatggc
//
LOCUS       DADLTY010000392         1070 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.398, whole genome shotgun sequence.
ACCESSION   DADLTY010000392 DADLTY010000000
VERSION     DADLTY010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.398"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="JN334_004472"
     CDS             complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="JN334_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HAY7440349.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          301 a          322 c          251 g          196 t
ORIGIN      
        1 tttaatccta tctgagtgtt tagcattgtt gcgctccttt catgtgcaat gccatataaa
       61 gccactggcg gagggagcgc gcagggggca gccagtcgcc gccgccccct gcacccccgg
      121 gctctggcga acaaaatcgc cgctgcgcgg taccttcagc ttatcccctt cggcaaccgg
      181 gtcgggcgcg aggtaacgtc ctgtaagacg cgccctcagc ccacatccat gtgggctgcc
      241 ccggccttcg gggaacgctt cagcgatttt gacgccacca atacccgtgc ggtttgtaat
      301 gtcgaagata aaacaaccag ggcagataaa atgccaatgc taattaatca cgcttttcca
      361 gcagagtccg gtaaatcaga ccaccgataa tgccgccgac aattggcacc acccagaaga
      421 accacagttg ttctaatgcc cagccgccct ggaagatagc aaccgcggtg ctgcgcgccg
      481 ggttaacaga agtgttagtc accggaatac tgattaagtg aatcagggtt aaggccagac
      541 caatagcgat cggcgcaaaa cctgccggag cgaatttgtc ggttgcaccg tggatcacta
      601 acaggaagcc cgcactcaat accagttcaa ctaccagcgc ggaaagcatg gaataaccgc
      661 ctggtgaatg ctcgccataa ccgttagaag caaaaccgct ggctgccgcg tcaaaacccg
      721 ttttaccact ggcaattaaa tacagcagcg ccgctgcaac aataccgccg acaacctggg
      781 caattacgta gccaacgact tcttttgccg gaaaacgtcc gccagcccat aaaccaatag
      841 tgaccgccgg gttaaaatga ccaccagaaa tatgaccaac agcgaaggcc atcgtcagaa
      901 cggtcaaacc aaatgccagc gccacgccgg caaaaccaat gcctaattcc gggaagcctg
      961 cggccagtac agcactacca cagccaccaa aaacaagcca gaaagtacca aaacattcag
     1021 ctgctaattt tctgaacata tccacctcaa ttaaaaattg accctgtgaa
//
LOCUS       DADLTY010000393         1069 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.399, whole genome shotgun sequence.
ACCESSION   DADLTY010000393 DADLTY010000000
VERSION     DADLTY010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.399"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(227..652)
                     /locus_tag="JN334_004473"
     CDS             complement(227..652)
                     /locus_tag="JN334_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440350.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(649..1032)
                     /locus_tag="JN334_004474"
     CDS             complement(649..1032)
                     /locus_tag="JN334_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440351.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          253 a          308 c          265 g          243 t
ORIGIN      
        1 ctcgcgtatg gttgattgac accacagtgc gaattcggcg aatgcgagat cacggtacga
       61 ttccgcacca cactaccaat caataccccg ggttttattc aggtaagcag gactgcggat
      121 atccggtgtc gcgcctttgt gtcacgaacg gggtgggtgc gaaacaccag ataaaataca
      181 ggttggcaat acctctgaac gccctgagca ggtcgggcat ttcggattaa gtactcgcac
      241 ctccgcagtc ctcaaacaag tctggctggt agctgtaaac agacttcgta cataccgctc
      301 tgcaataaat ccccgtacca caggcctcac agaacttctt cccatgaaaa aactgctcac
      361 acatcacaca atgccactcc agcacgaccg gtaatggcga taaaaacatc gccatatcct
      421 caatgtaagg gtgggacttc tccggattca gcaccacaca gtccgccttc tgttgctcac
      481 tcagggcatg taaatcatgc tcaaaccacg ccccctgagc atctgtctgc agaatcaacc
      541 ggccatggca ggaaaggcag aaacactgcc ggatatttct gctgagcgca gagtcggcac
      601 tgataatgtg ctcacccggc gtgatcccca gccccgtttt tataccgttc attctgctcc
      661 cccctcctca ctgaagcgcc cggtctggca gtgtgtgcag tactgctccc cataataatc
      721 gtggtgacat tgcctgcagt gccagctggc tttacgtact acgggtaagg tatccgggac
      781 gaattgctgc agacgtttaa tcaaccgtat ttctctgcgt tccggcctga cataagggca
      841 ctcatgaccg tgctccgtca gcccttcgtc agcgtgttca aaccagggcc gttcagtgtc
      901 gtattgcggg tggtatctga gtgcactgcc gcaaaggtgg caggtgtagc tatcgtaagg
      961 tgcagtctgt gcggtacggg caccggtcag acgtccgttg ccatcaagtg cgagaaaaga
     1021 ttttgcgtac atagtatatg ttcctcagcc gccagacgac acgcaggca
//
LOCUS       DADLTY010000394         1068 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.400, whole genome shotgun sequence.
ACCESSION   DADLTY010000394 DADLTY010000000
VERSION     DADLTY010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.400"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            18..1068
                     /locus_tag="JN334_004475"
                     /pseudo
     CDS             18..1068
                     /locus_tag="JN334_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580583.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          314 a          202 c          257 g          295 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc
      361 gttatctgag aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca
      421 ttacagcaga agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg
      481 tgaatgaagt tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca
      541 tgtcaggctg gaacctgtag agtcggtctt cactttttag gccagtgcgt aagtgttagt
      601 gtcagctcct gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg
      661 attcagcctc aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta
      721 gacagtgaga ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat
      781 ttgcatatgg aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat
      841 gtaaacggag gattttcttt ctcaaactca tggatagcag cagatgccga tggcactgaa
      901 caatttacgg ggatatattt tagaacgccg gcctcaacgc agtcacataa aattgtcagt
      961 ggtgttcata tcaacactgc aaacaaaaac acggctgcaa acaatcaaag tatagcgata
     1021 gaacagtcgg cgatcttcgt ctttgtaagt ggttgtacgt taactggt
//
LOCUS       DADLTY010000395         1066 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.401, whole genome shotgun sequence.
ACCESSION   DADLTY010000395 DADLTY010000000
VERSION     DADLTY010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.401"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..494)
                     /locus_tag="JN334_004476"
     CDS             complement(<1..494)
                     /locus_tag="JN334_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAY7440352.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEE"
     gene            complement(649..807)
                     /locus_tag="JN334_004477"
     CDS             complement(649..807)
                     /locus_tag="JN334_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAY7440353.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            complement(878..>1066)
                     /locus_tag="JN334_004478"
     CDS             complement(878..>1066)
                     /locus_tag="JN334_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HAY7440354.1"
                     /translation="AAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSV
                     SGSSAEGYNGQATLNVTF"
BASE COUNT          247 a          285 c          283 g          251 t
ORIGIN      
        1 ttttcctcaa tccggtcaaa aacccccacc acttcgtaag ccccttcgat aactttctcc
       61 accacatttc cccggtgtgg aacacgcact tcccccagag actgaccgca gacgcagcca
      121 ttctggcaga cgaatctgaa gtaacccggc agcatctggt agctggaggt accgtcatga
      181 gagttgagca gaataatttc agggacatgt tctccgttta tctctccggc ccgccgcaga
      241 cgcagcatgt gttttgtgta tccccggcgg cccgggtcgc gcacgcgggt ctggcaggcg
      301 aagaatggct gaaaaccttc ctgctgcagg ctttccagta cggtgatggt gggaatgtac
      361 gcatatcgtt tactgcggga agtatgccgg tcttcaccga aaatactggg aacatagtgc
      421 atcagttctt cgtgtgtcag cggacggtca cggcgtatct ggttcgcata accaaaacga
      481 ctggctagtc gcataatttg ctccttatcg gtggttacga tttactggtg taataaatga
      541 aaaagctgcg tctcccggag aagacgcggc ctgacagatg aaatgaatga cgtttattgt
      601 ctgagaagcc cttaactggc gagctgagta ttaagctgtg ttccggcatc accagcgcaa
      661 ctgaccttca gcattacgga taaccagccg ggaatatgtt ccctggtcat cttcagtaaa
      721 cacattgcgg taagctgttt tgacggcaac agcctgttcg cgtgagaaag ggccttcggg
      781 caataaacgt gatgtacaac ccggcatatc tgttgctccc tgaaagtaaa aagccccggt
      841 catgatgacc ggggcctgaa ggagagtgac ctgattatca aaaagtcaca ttcagcgtgg
      901 cctgaccgtt ataaccttca gcgctgctgc cgctgacgct gtgggcataa ccggcctgaa
      961 cgcccagggt gatattttcc cggacacggg cttccagtcc ggcctgcagg tccagtgacg
     1021 taccattctg tgacggtgag aacgtcatgt tactgccggc tgcggc
//
LOCUS       DADLTY010000396         1060 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.402, whole genome shotgun sequence.
ACCESSION   DADLTY010000396 DADLTY010000000
VERSION     DADLTY010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.402"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..384
                     /locus_tag="JN334_004479"
     CDS             <1..384
                     /locus_tag="JN334_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY7440355.1"
                     /translation="KDGYYLEPTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELA
                     NDTQYGLGAGVWSRNGNLAYKMGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETH
                     KMMLEHYQQTKCLLVSYSDKPLGLF"
     gene            complement(603..746)
                     /locus_tag="JN334_004480"
     CDS             complement(603..746)
                     /locus_tag="JN334_004480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440356.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            929..>1060
                     /locus_tag="JN334_004481"
     CDS             929..>1060
                     /locus_tag="JN334_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY7440357.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
BASE COUNT          279 a          227 c          262 g          292 t
ORIGIN      
        1 aaagacggct actacctcga accgacgatt ctgtttggtc agaacaatat gcgggtgttc
       61 caggaggaga tttttggccc ggtgctggcg gtgaccacct tcaaaacgat ggaagaagcg
      121 ctggagctgg cgaacgatac gcaatatggc ctgggcgcgg gcgtctggag ccgcaacggt
      181 aatctggcct ataagatggg gcgcggcata caggctgggc gcgtgtggac caactgttat
      241 cacgcttacc cggcacatgc tgcgtttggt ggctacaaac aatcaggtat cggtcgcgaa
      301 acccacaaga tgatgctgga gcattaccag caaaccaagt gcctgctggt gagctactcg
      361 gataaaccgt tggggctgtt ctgatataag aagctggtcg cattgggtat tcattgcctg
      421 atgcgacgct tacgcgtctt atcatgccta cgggaacctg accgtaggcc ggataaggcg
      481 tacacgccgc atccggcata aacaaagcgc acgttgttac aatttacgtt ttaaacctac
      541 gtgcgcaata acccagacta cgccaacaga agtgcgtttc tggggcgtgt tttgtcagga
      601 gtttaatctc gcgtttcgat ggtgagcggg cgaaccaggc cgtaaatcac cattggtgat
      661 ccgaggccaa tcagcaataa acggtagata attgaaggtt taatacgctt aatctgtact
      721 gtggatgttt tactggagtt attcattagt ttggccaata gttccactat ttcaatatga
      781 taaacctcgt cctgttgaaa aaccagacag cacatttcag aaagtatata aaaaacaata
      841 tgtcgttata taaataagga aattgcactg catcatggca tttcataata tgcgttaatt
      901 gttctttttt atattctttg agggaaatat gttcctggac tattttgcac tgggagtgct
      961 tatttttgta tttctggtga tattctatgg gattattatt ttacatgata ttccctacct
     1021 gattgctaaa aaacgtaatc atcctcatgc cgacgccatt
//
LOCUS       DADLTY010000397         1025 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.403, whole genome shotgun sequence.
ACCESSION   DADLTY010000397 DADLTY010000000
VERSION     DADLTY010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.403"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            1..555
                     /locus_tag="JN334_004482"
                     /pseudo
     CDS             1..555
                     /locus_tag="JN334_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          314 a          224 c          217 g          270 t
ORIGIN      
        1 atcaggtctg ggtcgcagat tcagttgtcc aggccttccg aaatcaaccg actgaaggcg
       61 ctggtcgcga aactgcagcg tatgcagttc ggtaaaagct cagaaaaact tcgcgcaaaa
      121 accgaacggt ggatacagga agcacaggag cgaatcagcg cacttcagga ggaaatggcg
      181 gaaacactgg gtgagcaata tgacccggta ctgccatctt ccctgcgcca gtcttcagcc
      241 cgtaaaccgt taccagcctc acttccccgt gccccccggg tcatccggcc ggaagaggaa
      301 tgctgtccgg cctgtggtgg tgaactcagt ccactgggat gtgatgtgtc agagcagctg
      361 gagcttatca gcagcgcctt taaggttatc gaaaaacaac gccctaaact ggcctgtcgc
      421 cggtgcgacc atatcgtgca ggcaccggtg ccttcaaaac ccattgcacg cagttatgcc
      481 ggagcggggc ttctggccca tgttgttacc ggaaagtatg cagaccatct gccattatac
      541 cgccaaagtg atctactatt ccacacagct atttagctct ctttttgttg agctcccatg
      601 attctttcat ttattgttac taattatttt atgttatcat gatgttagaa cacgctctgc
      661 tctgtgtctc tattttatct atcatattct gaggattaac tacataacaa ggcgcaagat
      721 atatatacaa aaacaaataa taccgttgca ataattcctt ttagtaaagg aggtgtgatt
      781 aatttcaggg gatttcagag taaaaattgt acaacatatt aaaatatgtt catttcgtta
      841 taatacacac aaggaaatat taagttagat aatatattct aaataaccat agataaaaac
      901 gcacataatg gggaatattt tatgaaaata cctgaagcag taaatcatat taatgttcag
      961 aataatattg atcttgttga tggaaaaacg aatccgaata aagcaacaaa agcattacaa
     1021 aaaat
//
LOCUS       DADLTY010000398         1019 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.404, whole genome shotgun sequence.
ACCESSION   DADLTY010000398 DADLTY010000000
VERSION     DADLTY010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.404"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(50..190)
                     /locus_tag="JN334_004483"
     CDS             complement(50..190)
                     /locus_tag="JN334_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440358.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..1000
                     /gene="queE"
                     /locus_tag="JN334_004484"
     CDS             329..1000
                     /gene="queE"
                     /locus_tag="JN334_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAY7440359.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          295 a          205 c          237 g          282 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt tcccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat
      601 tacgggtggt gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa
      661 cggttttagc tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac
      721 ctgggttacc gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc
      781 actggagcga gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact
      841 ggatgaacta ctggcaacgc tgaccgatga taaaccgcga gtcattgcac tgcagccgat
      901 tagccaaaag gatgatgcca cacgattgtg cattgaaacc tgtattgcgc gtaattggcg
      961 tttgtcgatg caaacacata aatatttaaa tattgcctga ttacacattt ataagcgtt
//
LOCUS       DADLTY010000399         1005 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.405, whole genome shotgun sequence.
ACCESSION   DADLTY010000399 DADLTY010000000
VERSION     DADLTY010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.405"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(22..825)
                     /gene="dkgB"
                     /locus_tag="JN334_004485"
     CDS             complement(22..825)
                     /gene="dkgB"
                     /locus_tag="JN334_004485"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HAY7440360.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          264 a          225 c          233 g          283 t
ORIGIN      
        1 cggaggagct gtcagagagg cttaatccca ttcaggagcc agaccttccg ggctaaccag
       61 gcggtcgttg caatccagtg cggcgatcgc ttttttatct tcggcatcaa gctgtaaatt
      121 ttgtgcctta agattacttt ccaggttttt acgtttagta gaagaaggaa ttactgagta
      181 accttccccc atagcccacg ccagaatcac ttgtgccgga atcgcattgt gtttagctgc
      241 aatacgagca ataacctcat ctttcagtgc cttaccataa gccagcgtca tataggaagt
      301 gatatgaata ccgtgctgtt tagcccaggc aaccacttta cggttttgca gataaggaga
      361 gagttcaatc tggttggtag cgatgttttc agcgccaaca gcagcaatca ctttttccat
      421 caatgggatc gtgaagttgg aaataccgat ctcacgcgtc agcccttgtt ttttggcttc
      481 cagcagcgcc tgcataaact cttcaacaga gacttcatcg tttggtgacg gccagtggat
      541 tagcgtcaga tcaacataat cggtacgcaa tttttgcagg ctctctttca gactcgggat
      601 caatttgtct ttgctgagat tttcaatcca gattttagtg gtgatgtaga gttcatgacg
      661 tggcacgcca ctttctgcaa tcgcctgacc tactgcggct tcgttatcat agatttgtgc
      721 ggtatcaatt gcgcgataac caagttcaag cgccgttttc acagatgaaa taacaacgtc
      781 gtctttcaga cggaaagtac ctaaaccaaa tgcagggata gccataatat gcctctttta
      841 atgctatttt cgttaactga ggtgattatg ggcttcagac tgttgcggga aaagagcgta
      901 aaacgcagag gatttttgcg attctagcaa taatagatat ccagacgcaa aaaaacatcg
      961 agcgttaact cgaggcttct taataagtgg cggaacggac gggac
//
LOCUS       DADLTY010000400          962 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.406, whole genome shotgun sequence.
ACCESSION   DADLTY010000400 DADLTY010000000
VERSION     DADLTY010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.406"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..496
                     /locus_tag="JN334_004486"
     CDS             <1..496
                     /locus_tag="JN334_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAY7440361.1"
                     /translation="LHASTNAPGSDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQK
                     THYSDTRWHEDSVRNRWFSVMAGLSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDN
                     KGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGV
                     GYKF"
     gene            560..>962
                     /locus_tag="JN334_004487"
     CDS             560..>962
                     /locus_tag="JN334_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7440362.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAA"
BASE COUNT          210 a          221 c          319 g          212 t
ORIGIN      
        1 tcttcatgcc tcgacgaacg ctcccggcag tgatgatctg aacgggatta acgtgaaata
       61 ccgttatgag tttacggaca cgctggggct ggtgacgtca ttcagctatg ccaacgctga
      121 agatgagcaa aaaacgcatt acagtgatac ccgctggcat gaggattccg tgcgtaaccg
      181 ctggttcagc gtgatggcgg ggctgtctgt gcgcgtgaat gaatggttca gcgcgtatgc
      241 gatggcgggc gtggcttaca gccgtgtgtc gaccttctcc ggggattatc tccgcgtaac
      301 tgacaacaag gggaaaacgc acgatgtgct gaccgggagt gatgacaacc gccacagcaa
      361 cacgtctctg gcgtgggggg ctggcgtgca gtttaacccg accgaatccg tggccattga
      421 ccttgcttat gaaggttccg gcagtggcga ctggcgcact gacggtttca tcgtgggtgt
      481 cggttataag ttctgattag ccaggtaaca cagtgttatg acagcccgct ggttcaggcg
      541 ggctttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag atggggcggg
      601 taaacctgtc gtaaattgtg cgattgaact gcgggccaga agaaccagtc cgaccgttgt
      661 ggcacacgtt gttgccactt gcgtgacgga caataacggt gcttatgtga ttgaggctga
      721 gccggggtat tacgaggttg cgcttcactg taacggctgg cagccaaccc gtgtcgggga
      781 tattgatgtg gcaccgactg atgcaccggg gacactgaac gcgtttctga atgcaccaaa
      841 ggatggtgat ttacgtccgg aggtgatgaa gcgctttgag gaaatggtgg cgcaggcgca
      901 gcagagtgcc ggggctgcag ccggaaacgc acagcagacg gcgcaggatg tggcggcagc
      961 cg
//
LOCUS       DADLTY010000401          932 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.407, whole genome shotgun sequence.
ACCESSION   DADLTY010000401 DADLTY010000000
VERSION     DADLTY010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.407"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            47..319
                     /locus_tag="JN334_004488"
     CDS             47..319
                     /locus_tag="JN334_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440363.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVLLLQLA"
BASE COUNT          245 a          181 c          216 g          290 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aa
//
LOCUS       DADLTY010000402          921 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.408, whole genome shotgun sequence.
ACCESSION   DADLTY010000402 DADLTY010000000
VERSION     DADLTY010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.408"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            1..198
                     /locus_tag="JN334_004489"
                     /pseudo
     CDS             1..198
                     /locus_tag="JN334_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            298..>921
                     /locus_tag="JN334_004490"
     CDS             298..>921
                     /locus_tag="JN334_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAY7440364.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          292 a          217 c          205 g          207 t
ORIGIN      
        1 tggctacgta attaaaacag aaaataaaga aaccccattc actgtagttg atatcgacgg
       61 tctatcagga aactttaaaa ctcttgttga aggtgccaca aaaatgtgcc tagtttacat
      121 cggaaataat ctgcccgcag aaaaaaagcc gaatttctgg caaatctgat tgcaatgaaa
      181 ttaaaaggtg aaatctgaaa aaaagaaagc ctgcacagtg tgcaggcctg agtgaagaac
      241 ctgggacatt tattcatcac tcgcagtaat tttaatctga gttgaggtta aaaaacaatg
      301 agcacaaaac cactcttcct gttacggaaa gcgaaaaaat catccggtga acctgacgtc
      361 gtcctgtggg caagcgacga ttttgaatcg accagcacca ctctggacta tctgctcgtt
      421 aagtcaggta aaaaactgag caactatttt aaagctgttg ccacaaattt tcctgtcgtt
      481 aatgaccttc cccctgaagg tgagatcgat tttacctgga gtgaacgcta tcaactcagc
      541 aaagactcca tgacatggga actaaaaccg ggagcagcgc cagacgacgt tcaccaccag
      601 gataatgctc aagaaaccaa agaactggcg ggaggccagg aagaaaacgc gcaggcagac
      661 gcccacgagg attgccagga ttgcgaagtc tctgtagcca ctttgcggtt cacacagcgt
      721 cttctgcaca tttttacgta tgctgccggg gatctgaaat acctgcatca tgccacccgt
      781 gaacaacgca aacacattac tgctcttgag atggatcagg aaaacagcta tgtccagaat
      841 ctgctgttgg ccatacgcgg catggcagaa ccgacaactc tggataatgc cgccctgctc
      901 cgcctgactg atgcaattaa g
//
LOCUS       DADLTY010000403          909 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.409, whole genome shotgun sequence.
ACCESSION   DADLTY010000403 DADLTY010000000
VERSION     DADLTY010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.409"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            487..>909
                     /locus_tag="JN334_004491"
     CDS             487..>909
                     /locus_tag="JN334_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440365.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNVLRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSLEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNF"
BASE COUNT          315 a          171 c          170 g          253 t
ORIGIN      
        1 atcccgtact ctctggccag ttgggccacg caggccccta gctggtaaga tttttcaata
       61 agatcaagct taaactccat agagtcatag agtaagaggc acgaatcctg cgggggcgaa
      121 gagaatctga cattgataga gtccattaac gttataagag gactctatca tcgccaatca
      181 tcagacattg aaatagacga cactgagatg acgcttacaa ttcttttggt ttctgtaata
      241 agtaatctga catcccccgt gcccagaaga ggagagggat atcaagccca agggttaata
      301 attgatacta cggttaaaat tgccttcagt aaaggaaacg tgattaaatt caggggattt
      361 cagagtgaaa accatacaac atattaaaat ctgttcattt agctataata catacaagaa
      421 agtgtccagt tagataatgt tatctaaata accacagata aaaacgcaca taattggggg
      481 ggggggatga aaatacctga agcagtaaat catattaatg ttcagaataa tattgatctt
      541 gttgatggaa aaacgaatcc gaataaagca acaaaagcat tacaaaaaaa tgtattacgc
      601 gtaacaaact catcttcttc tggcataagt gaaaaacatt tagatcactg tgctaatact
      661 gtgaaaaact ttttaaggaa gtctatagca gcgcaaagtt atagcaaaat gttttctcaa
      721 gggaccagtt tcaaatcact aaatctttct cttgaagcgc catctggagc acggtcatca
      781 tttcggtcac ttgagcatct cgataaggtg tccaggcact atatctctga gataatacaa
      841 aaagttcatc ctctttcttc agacgaacgt cacttacttt ctattattat taattctaat
      901 tttaacttc
//
LOCUS       DADLTY010000404          899 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.410, whole genome shotgun sequence.
ACCESSION   DADLTY010000404 DADLTY010000000
VERSION     DADLTY010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.410"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..>899)
                     /locus_tag="JN334_004492"
     CDS             complement(<1..>899)
                     /locus_tag="JN334_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAY7440366.1"
                     /translation="PMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFGVSSGLSI
                     SKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIWALFLPVI
                     IIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTSVVMFLVA
                     SAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTPTVLILTP
                     VLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDAVRGVFPY
                     VLVLYSLLVVFVFIPDLIILPLK"
BASE COUNT          285 a          211 c          230 g          173 t
ORIGIN      
        1 tttcaaaggg aggatgatga gatcgggaat aaaaacaaat acgactaata atgaatataa
       61 aaccaggacg taagggaaaa cgcctctgac tgcatcatcg aatttgagtt ttgccacccc
      121 tgaaataacg ttaagtacat taccgatagg cggtgtaatt aagccgattg agcagttaat
      181 gataaacatg acaccgaaat aaatcggatc gattcctgcc tctttaacta aaggcattaa
      241 taccggggta agaattaata ccgtcggcgt taaatccatg accatgccga caatcaggat
      301 agccaccata atgacgataa acagcagacg cggtgaatcg accaacggtt gcagtaaatc
      361 agaaaccatc atcggcagtt cagcaatggt aatcagccag gcggaaactt gtgccgaggc
      421 caccagaaac ataaccactg aggtggtttt cgccgcgcca atgagtacat gccaaagtgt
      481 ggcgaaggtc atttcacggt aaataactgt ggcgacaaac agcgcataga aagcagcaac
      541 ggccccggct tcggtagggg taaacagacc ggagcgaaac ccgccaataa tgatgactgg
      601 aagaaacagc gcccagatac cggagacaaa agagtgccag atttcctgca tcgttgcttt
      661 ttgctggcgg ggtaaattaa ggcgactggc ctgccgccac caggtaagca tcagcgttgc
      721 gcccatcatt atgccagggg caatgcccgc cataaacagc ttactgatgg ataatccgct
      781 ggaaacaccg aagataataa acggaatgga agggggaata attggcgcga taatgccgcc
      841 agaggcaatc agccccgccg cccggttgac cgggtagttg gcgctgcgca tcatcggca
//
LOCUS       DADLTY010000405          898 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.411, whole genome shotgun sequence.
ACCESSION   DADLTY010000405 DADLTY010000000
VERSION     DADLTY010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.411"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            157..822
                     /locus_tag="JN334_004493"
     CDS             157..822
                     /locus_tag="JN334_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7440367.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          287 a          185 c          195 g          231 t
ORIGIN      
        1 aaagcttatt ttcagcttta attaactaac cacgtcattg ttctgctgct aataacaaca
       61 aaggtgggtg gtttatgatt attcacttta atacactagg tgaactcctt ctgccatgca
      121 ggcagggttg gacagaaaac acgcgaagga attaaaatgc ctggaacggg aaaaatgaaa
      181 catgtcagtt tgactctgca ggttgagaac gacctgaaac atcagcttag tattggcgca
      241 ttaaaacctg gcgcacgcct gattactaaa aatctggcgg agcaattagg tatgagtatt
      301 acacctgtgc gtgaagcatt attacgtctg gtttcggtga atgcgctttc tgtcgcacct
      361 gcacaagcat ttacagtacc ggaagtgagg caaagtcagt tagacgaaat caatcggatc
      421 cgctacgaac tggaattaat ggcagttgcc ctggctgttg aaaacctctc ctcgcaagac
      481 atagcagaac ttcaggaatt gctcgggaaa ttacagcagg cgcaagaaaa gggcgacatg
      541 gaacaaatca ttaatgtgaa caggctattt cgcttagcga tttatcatcg ctcaaatatg
      601 cccatcctgt gtgagatgat tgagcaactg tggatcagga tgggacctgc tttacattat
      661 ctttatgaag cgattaaccc agcggaatta cgggagcgca tagaaaacta tcatctatta
      721 ctcgctgcgt taaaagcaaa agacaaagag ggatgcaaac attgtcttgc tgaaattatg
      781 caacaaaata ttgctatttt ataccagcaa tacaatcgtt aaattaaatg cccggcaaat
      841 accgggcatc aagtaattac tcaaatcgcc acgcaatatt catgccgaca ccataatt
//
LOCUS       DADLTY010000406          870 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.412, whole genome shotgun sequence.
ACCESSION   DADLTY010000406 DADLTY010000000
VERSION     DADLTY010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.412"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..>870
                     /locus_tag="JN334_004494"
     CDS             <1..>870
                     /locus_tag="JN334_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAY7440368.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSE
                     NGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVTL
                     AYRPLNDTQRKIV"
BASE COUNT          263 a          153 c          209 g          245 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa
      721 gagtcttggt gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt
      781 gaccgatatt agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc
      841 actaaatgac actcagagga aaattgttga
//
LOCUS       DADLTY010000407          858 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.413, whole genome shotgun sequence.
ACCESSION   DADLTY010000407 DADLTY010000000
VERSION     DADLTY010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.413"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(49..624)
                     /locus_tag="JN334_004495"
                     /pseudo
     CDS             complement(49..624)
                     /locus_tag="JN334_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            628..858
                     /locus_tag="JN334_004496"
                     /pseudo
     CDS             628..858
                     /locus_tag="JN334_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          198 a          238 c          200 g          222 t
ORIGIN      
        1 gtcaacgacg gatgaaaagt ggctcttccg caacctgggt ggagattttc atgccagtgg
       61 actcattacc ctctgcctga gtagttcaaa tcctgctcga ccatacatct ggcgtttcag
      121 catcttcttc aggcgattta catgaccttc gacaacacca ttactccagc gactactgat
      181 tgcctcacat atagctgcag catcagcttc catccccgcc gccacgcgcc gaaattctgc
      241 tgaaccgctt tcgtggactc gagtgaacca actgcttagc tgtgatttat tttgggtttt
      301 cagaatacgg tagaactcga gtgccagttg ctgcgctatt ttcagctcag gttctttttc
      361 acacatcaga ctaataaatc gggaagcata attttcttca tccctggtta ttctccaggg
      421 catcaaccag cggcttaccc gggagactga aggaagtctg acggcagtag taaccggtgg
      481 gttccagcct ttacgccatt tggcaaccgc atccctgacg atggtttcac tgcctgtaaa
      541 tccccgggcc accatttccc gccatatccg gctggcgtta taattaccac tttctcgttg
      601 ctcttttaac cactccctcc atggtgtcag cgaaaaacga aaccgctgtt gaaatccctg
      661 gaaagctggt tgcggaaaag atgaagaccc agtcgcgaca ctcagagttg gcgaaggcgt
      721 tcgcgtacgc acttaaccag tggccggcac tgacgtacta tgcgaacgat ggctgggtgg
      781 aaatcgacaa caacatcgct gaaaatgcct tgcgggcggt cagtctgggt cgtaaaaact
      841 tcctgttctt cggctctg
//
LOCUS       DADLTY010000408          832 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.414, whole genome shotgun sequence.
ACCESSION   DADLTY010000408 DADLTY010000000
VERSION     DADLTY010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.414"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..327
                     /locus_tag="JN334_004497"
     CDS             <1..327
                     /locus_tag="JN334_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HAY7440369.1"
                     /translation="VSETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNG
                     LLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVI
                     LKITYN"
     gene            complement(370..570)
                     /gene="glgS"
                     /locus_tag="JN334_004498"
     CDS             complement(370..570)
                     /gene="glgS"
                     /locus_tag="JN334_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HAY7440370.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          252 a          184 c          155 g          241 t
ORIGIN      
        1 gttagtgaga cgtttagtgt agtcatgaca aaagatcctg gtgcggcctg tactgagcaa
       61 ttcaatattt tagggagctt tttcactacg gatattttga gtgattatag ccatctggat
      121 atgggtaatg gactgctatt gaagatattt cataaagatg gaacagcaac ggaatttaac
      181 cgcttctcac aatttgcttc tttttcatcg tctagtgcac cttcggtcac cgcaccattc
      241 agggcagaac ttagtgcgaa cccggcagag actgttgttg agggaccgtt tagtaaagac
      301 gtaatcctca aaatcaccta taactagcat ctaatacaaa cactaaaacg ggccatcagg
      361 cccgttatat cagtgctcta actccagctc tcttgcctgc atcatctgtt ggcaatacca
      421 ggcataacgt gcgcaaaacc aagttctatg ttcttcatcc atgttaccag taacagccag
      481 aatatcgacc gggcgacctt ctgcgtgcat tctggcaaaa ctacgcgcca ggaaatcgaa
      541 attattcaaa gaattaagac tatgctccat aagcacatcc tccggttatc gcataccttt
      601 taaggccata tgctttttct gatttcagta ttccccaatc agtgtagtgc ctttaaacat
      661 aacgtgcgta aatatattgt ccccgatcac actttttagt gaaaaataaa aaaccatatt
      721 aaaatacttt ctcttccaga acttctctaa catgtgttat tattaacaaa gacaaatgta
      781 agcagggaaa ctattccaag gtaaatattg cgtcactaaa tggacattgg ag
//
LOCUS       DADLTY010000409          804 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.415, whole genome shotgun sequence.
ACCESSION   DADLTY010000409 DADLTY010000000
VERSION     DADLTY010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.415"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..>804
                     /gene="narH"
                     /locus_tag="JN334_004499"
     CDS             <1..>804
                     /gene="narH"
                     /locus_tag="JN334_004499"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY7440371.1"
                     /translation="TCNNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQEEWQGGWVR
                     DVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYYEPFTFDYEHLHSTPEGKHIPTARP
                     RSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNFEAMQKEMYGQFENTFMMYLPRLC
                     EHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKSE
                     KCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEASSTEREVDL"
BASE COUNT          212 a          209 c          226 g          157 t
ORIGIN      
        1 gacctgtaac aacgtctgga ccggacgcga aggcatggag tacgcatggt ttaacaacgt
       61 cgaaaccaaa ccgggcattg gttatccgaa aaactgggaa gatcaggaag agtggcaagg
      121 cggctgggtc cgcgatgtga atggcaagat ccgtccgcgt ctgggcagca agatgggcgt
      181 aataaccaaa atcttcgcca acccggtggt gccgcagcag attgatgatt actacgaacc
      241 tttcaccttc gactacgaac atttgcatag cacaccggaa ggcaaacata tccctactgc
      301 tcgcccgcgt tcactgattg acggcaaacg gatggacaaa gtgatctggg gcccaaactg
      361 ggaagaactg ctgggcggcg agtttgaaaa acgtgcccgc gaccgcaact tcgaggccat
      421 gcaaaaggag atgtacgggc agtttgaaaa caccttcatg atgtacctgc cgcgcctgtg
      481 cgaacactgc ctcaatccca gctgcgtggc gacctgccca agcggcgcta tctacaaacg
      541 cgaagaagac ggcattgtgc tgattgatca ggataaatgc cgtggctggc gtttgtgcat
      601 tagcggttgt ccgtacaaaa aaatctactt caactggaaa agcggcaagt cagaaaaatg
      661 tatcttctgt tatccgcgaa ttgagtcagg ccaacccacc gtgtgctcag aaacctgcgt
      721 gggtcgcatc cggtatctgg gcgtgctgct ttacgacgcc gaccgcatcg aggaagcgtc
      781 gagcaccgag cgcgaagttg acct
//
LOCUS       DADLTY010000410          782 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.416, whole genome shotgun sequence.
ACCESSION   DADLTY010000410 DADLTY010000000
VERSION     DADLTY010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.416"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(570..>782)
                     /locus_tag="JN334_004500"
     CDS             complement(570..>782)
                     /locus_tag="JN334_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440372.1"
                     /translation="WLSGDSGGQSTGYKDSDRLNYIEIGTWYYFNKNMNIYTAYQINL
                     LDKSDYVLAHGLNTDDQLAVGIVYQF"
BASE COUNT          213 a          131 c          201 g          237 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 ca
//
LOCUS       DADLTY010000411          780 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.417, whole genome shotgun sequence.
ACCESSION   DADLTY010000411 DADLTY010000000
VERSION     DADLTY010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.417"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..666)
                     /locus_tag="JN334_004501"
     CDS             complement(<1..666)
                     /locus_tag="JN334_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HAY7440373.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(656..>780)
                     /locus_tag="JN334_004502"
     CDS             complement(656..>780)
                     /locus_tag="JN334_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440374.1"
                     /translation="RVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT          251 a          203 c          177 g          149 t
ORIGIN      
        1 tggcagtgct attttgattc gacgttgatc aagtttctga taattccaac gacggtcgac
       61 gggcaccatc cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg
      121 atgctgccat gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cacagacttt
      181 acgccacctc acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat
      241 atgcagcgaa ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca
      301 tcctgtcacc ccactcactt caggacggat aaaactaaac ttcagcgggt agagaaaaaa
      361 accgcagcag gccattaccg ttgccagaat aagccggtgg acaagtcggc gttgttcgaa
      421 tgtcgagcta caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac
      481 aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tggggatatc
      541 atggctgtta aggtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa
      601 aaacggggcc agcaacaata accagccagc gccttgtagc aatacgttcc gacgttcagt
      661 tatcacgacg caccgccatc gaaaccgtaa aaatgcccca cacatcaatc cgttgagtgc
      721 atttatcaaa tccggcatta cgcacgagcg aatccatctc cccttgcgaa cgcacgcgca
//
LOCUS       DADLTY010000412          779 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.418, whole genome shotgun sequence.
ACCESSION   DADLTY010000412 DADLTY010000000
VERSION     DADLTY010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.418"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(142..>779)
                     /locus_tag="JN334_004503"
     rRNA            complement(142..>779)
                     /locus_tag="JN334_004503"
                     /product="16S ribosomal RNA"
BASE COUNT          168 a          228 c          189 g          194 t
ORIGIN      
        1 cctaggggat tcgaacccct gttaccgccg tgaaagggcg gtgtcctggg cctctagacg
       61 aaggggacgt atcagtctgc ttcgcaagac gccttgcttt tcactttcta tcagacaatc
      121 tgtgtgagca ctacaaagaa cgcttcttta aggtaaggag gtgatccaac cgcaggttcc
      181 cctacggtta ccttgttacg acttcacccc agtcatgaat cacaaagtgg taagcgccct
      241 cccgaaggtt aagctaccta cttcttttgc aacccactcc catggtgtga cgggcggtgt
      301 gtacaaggcc cgggaacgta ttcaccgtgg cattctgatc cacgattact agcgattccg
      361 acttcatgga gtcgagttgc agactccaat ccggactacg acgcacttta tgaggtccgc
      421 ttgctctcgc gaggtcgctt ctctttgtat gcgccattgt agcacgtgtg tagccctggt
      481 cgtaagggcc atgatgactt gacgtcatcc ccaccttcct ccagtttatc actggcagtc
      541 tcctttgagt tcccggccgg accgctggca acaaaggata agggttgcgc tcgttgcggg
      601 acttaaccca acatttcaca acacgagctg acgacagcca tgcagcacct gtctcacggt
      661 tcccgaaggc acattctcat ctctgaaaac ttccgtggat gtcaagacca ggtaaggttc
      721 ttcgcgttgc atcgaattaa accacatgct ccaccgcttg tgcgggcccc cgtcaattc
//
LOCUS       DADLTY010000413          763 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.419, whole genome shotgun sequence.
ACCESSION   DADLTY010000413 DADLTY010000000
VERSION     DADLTY010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.419"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            459..>763
                     /locus_tag="JN334_004504"
     CDS             459..>763
                     /locus_tag="JN334_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7440375.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT          259 a          152 c          127 g          225 t
ORIGIN      
        1 tgttgaaaca aatgacggta gagaatgtta acactgccgt taaacaatat ctttctcatc
       61 cggtaaatac ttataccgga gtattattgc caaaataata acagccccgt caacacatcg
      121 ttggcgggga ttttagcaat attcgctatt tttatgtaat aattttataa atgcgttcaa
      181 aataataacc aagtactaat agtgatattt taatacctga tttttacgtg ataattcagg
      241 aggcacagaa agcgcataaa aataacaaca taaaacacct tgccaccacc caagaattct
      301 ctattacatt gtttttaatg aaaaaatatc attccaacaa tatctcacga taaataacat
      361 taggattttg ttatttaaac acgagtcctt tgcacttgct tactttatcg ataaatccta
      421 cttttttaat gcgatccaat cattttaagg agtttaaaat ggataagaag caagtaacgg
      481 atttaaggtc ggaactactc gattcacgtt ttggtgcgaa gtctatttcc actatcgcag
      541 aatcaaaacg ttttccgctg cacgaaatgc gcgacgatgt cgcatttcag attatcaatg
      601 atgaattata tcttgatggc aacgctcgtc agaacctggc cactttctgc cagacctggg
      661 acgacgaaaa cgtccacaag ttgatggatt tatccattaa caaaaactgg atcgacaaag
      721 aagaatatcc gcaatccgca gccatcgacc tgcgttgcgt aaa
//
LOCUS       DADLTY010000414          759 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.420, whole genome shotgun sequence.
ACCESSION   DADLTY010000414 DADLTY010000000
VERSION     DADLTY010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.420"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            103..>759
                     /locus_tag="JN334_004505"
     CDS             103..>759
                     /locus_tag="JN334_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7440376.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     M"
BASE COUNT          263 a          144 c          131 g          221 t
ORIGIN      
        1 agcaatacct ggagaaagag tacaaagcaa actgtattaa acttaataag ttatattgta
       61 tttcactaga gttcttatcg ttgataagaa attctggtta taatgattaa atcaacgaat
      121 atacaggtaa tcggttctgg tattatgcat caaataaaca acatacactc gttaactcta
      181 ttttctttgc ctgtgagttt gagtccatct tgtaatgaat attatttaaa agtctggagt
      241 gaatgggaaa agaacggtac cccaggcgag caacgcaata tcgccttcaa taggctaaaa
      301 atatgtttgc aaaatcaagg ggcggaatta aatctatctg agttagattt aaaaacatta
      361 cctgatttac cgcctcagat aacaacactg gaaataagaa aaaacctatt aacatatctc
      421 cctgatttcc caccaatgct taaggtaata catgctcaat ttaatcaact ggaaagctta
      481 cctgccttac ccgagacgtt agaagagctt aatgtgggtg ataacaagat aaaagaatta
      541 ccatatcttc ctgaaactct aactcattta cggatccata ataaccgatt gcatattctg
      601 ccactattgc caccggagct aaaattactg atagtttctg gaaacagatt agacagcatc
      661 cccccctttc cagataagct tgaagggctg gctctggcta ataattttat agaacaacta
      721 ccggaattac cttttagtat gaacagggct gtgctaatg
//
LOCUS       DADLTY010000415          759 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.421, whole genome shotgun sequence.
ACCESSION   DADLTY010000415 DADLTY010000000
VERSION     DADLTY010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.421"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..>759
                     /locus_tag="JN334_004506"
     CDS             <1..>759
                     /locus_tag="JN334_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440377.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          184 g          209 t
ORIGIN      
        1 gagcgcattc tgaccgaccg tgaaaatggt gaaaaaggcg ataattctca agatacctct
       61 tatgcccgtg taggcgtgaa aggtgaaact cagatcaacc ctgaaatgac tggttacggt
      121 cagtttgaac tagacctgga agcatcaaac agacacaacc ctgaccaaac tcgtttggca
      181 tatgctggtt tgagttacaa agattttggt tcattcgact acagtcgtaa tgtcggtgtc
      241 gcgtatgatg cagaagcatt tactgatatg tttgttgagt ggggcggtga ctcatgggct
      301 ggtactgacc tgttcatgac caaccgtacc aacggtgttg caacctatcg taacacagat
      361 ttctttggca tggttgaagg tctaaatttt gctctgcaat atcagggtaa aaatgaaggt
      421 acaggcaact acaaagcaaa tggcgatggc catggtttat ctgcaactta cactattgac
      481 ggtttctcat ttgctggtgc ttatgcaaac tctgaccgta ctgattggca gtctggtgat
      541 ggtaaaggcg aaagagccga agtttgggcg ttgtcaacaa aatatgacgc taacaatgtt
      601 tatgctgctg tgatgtacgg tgaatctcac aacatgaatt ccgacgacgg tgatgttgtg
      661 aacaaaacac aaaacttcga agctgttctg caatatcagt tcgactttgg tctgcgtcca
      721 tccatcggtt actcctattc taaagcactg gacgtagca
//
LOCUS       DADLTY010000416          724 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.423, whole genome shotgun sequence.
ACCESSION   DADLTY010000416 DADLTY010000000
VERSION     DADLTY010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.423"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..>724)
                     /locus_tag="JN334_004507"
     CDS             complement(<1..>724)
                     /locus_tag="JN334_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440378.1"
                     /translation="GHGKACPLNEPILLADGTWTTHGNVKIGDQVASVDGNPSTVTGI
                     FPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEKRRVIDTFGLKRLSETKRHK
                     NGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKFSNVEPYMIERMKAELPDYN
                     FSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFIPRVFFSANKSTRIGMLCGL
                     LETDGYVEKDGTLVFSSASEELR"
BASE COUNT          180 a          209 c          145 g          190 t
ORIGIN      
        1 cgcagttctt cacttgctga ggaaaaaaca agcgttccat ccttctcgac atacccatcc
       61 gtttcaagca gaccacacag catgcctata cgcgttgatt tatttgcgga aaaaaacaca
      121 cgagggatga atttgttttt tgctgtgcac cccattagtc cataacctcg cagggtctcc
      181 atgagtggat ttacctgacc acgcgccgtt gatatcagcc agtccttacc atctccagag
      241 aagttgtaat caggcagttc agccttcata cgctcgatca tgtatggctc gacgtttgaa
      301 aacttcacgc tcccattgct aagactacca tccccgagaa gggagccgat aacccatgct
      361 aatggctctg agtggtcgcc aaagtcacca gttatttcag gaatcctaac gccatttttg
      421 tgcctcttcg tttcactcaa ccgcttcaac ccgaaggtat ctatcacgcg gcgtttctcg
      481 cctttcgtga atccacgact agtgacctcc cataggtgat cgcctgcgca atcaacataa
      541 cgaccatctt caaatgtgac tcggtaaaca tctctaacac cctgcgggaa tatcccagtg
      601 acagttgacg gattgccgtc tactgacgcc acctgatcgc caatttttac attcccgtga
      661 gtagtccatg tcccatcagc taaaagaata ggctcattca gtgggcatgc ctttccgtga
      721 ccgt
//
LOCUS       DADLTY010000417          717 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.424, whole genome shotgun sequence.
ACCESSION   DADLTY010000417 DADLTY010000000
VERSION     DADLTY010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.424"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(54..685)
                     /locus_tag="JN334_004508"
                     /pseudo
     CDS             complement(54..685)
                     /locus_tag="JN334_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001691565.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          207 a          147 c          138 g          225 t
ORIGIN      
        1 cagtgggtat atctgttcag aatatttcac gtagcacacg gatatataag aaatcattct
       61 tcctctggat agcttgaact taagagcata tcctgcgtga attggtcatt agctgattga
      121 ttgaagctaa agaaagatgg tgcatccggt ctaacagttg cttcaccagt gatattaact
      181 gataaaggct tcttagtttc tgctgttaaa gcgtctacct ttcttcctaa ctctgataca
      241 gccatagcta attgagcatt gctattaacc atgtcacctg tgatagctga tggagttgat
      301 tgtgtgttat ttacgaagtt attcatcggt tcttggaagt tagcaatttt attagttgca
      361 ccattaaacc agtcagcgat agaactattg gcttagatga atcacctttg tattcagggt
      421 tgaagtttgt ttctgtgcca atacctgatt gatgtaagtt cggattctta gctgcttcag
      481 ggtttaaatt atcaagagca aaatttacca actcagcata aaggacaact ctaccgactt
      541 tatttagcgc accacctgaa gaaccagcct tgccagcttt agctttatta acagcgtcac
      601 cgaatgaagt aacagcgttt gcaaggttta acaagcctcc tgctacattt gcagcctttt
      661 gagcagtaac agccacagca agcatcttca gaccatcaat taacccatca gataaac
//
LOCUS       DADLTY010000418          704 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.425, whole genome shotgun sequence.
ACCESSION   DADLTY010000418 DADLTY010000000
VERSION     DADLTY010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.425"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..443)
                     /locus_tag="JN334_004509"
     CDS             complement(<1..443)
                     /locus_tag="JN334_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAY7440379.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          214 a          172 c          131 g          187 t
ORIGIN      
        1 tcatctggat aatcggcatg agcaataaca tgcttgccgg tgcgactgtg tacttcaaga
       61 ataaagggca aatgctgaca tttttccagc gccgttttcg cttgtttctg ttgattatct
      121 gccagcgcaa taaaccagtc gccgccattc atcaaccaca aagacatctg ctggaatgcc
      181 agcgcatcca tcgccatctg ttcatgattg cctcttaccg cacgaaccca atgttgttcc
      241 agtaactgca gacaacgtaa actttgcggc ccacgatcga taacgtctcc cactgagata
      301 agtaaatctc gccacggatc aaaacgacaa tgccataatt tgcggcgcaa ctgctcaaga
      361 caaccgtgta tatcgccaga aagccagata tgtcgccatt gatgacccgc aattctctga
      421 taaacgggcg caggctgttt catcaatatt ttcctcccgc gctaaagatc acataatctt
      481 aacaagaatg ttaaaaaacg ctggactcag acagtagagt gtgtgttatg gttgactata
      541 aagtcagcga aggaaatgct tctggctttt aacagataaa aagagaccga acatgattcc
      601 tgttttcgtc aacaaacaat aaaacccttt aaaattaatg cgttgaataa atttacgttc
      661 atcttttaat ccctgacacg taccattgca tattaataca ttca
//
LOCUS       DADLTY010000419          695 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.426, whole genome shotgun sequence.
ACCESSION   DADLTY010000419 DADLTY010000000
VERSION     DADLTY010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.426"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            43..>695
                     /locus_tag="JN334_004510"
     CDS             43..>695
                     /locus_tag="JN334_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HAY7440380.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFY"
BASE COUNT          169 a          184 c          185 g          157 t
ORIGIN      
        1 tggtgataaa cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc
       61 cggcattttc agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc
      121 agttaccgtg agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg
      181 atttaccgct gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg
      241 cgtaaccctt ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc
      301 cgctgggcgt atctgtaccg ggccgtcgac agccggggcc gcactgtcga tttttatctc
      361 tcctcccgtc gtaacagcaa agctgcatac cggtttctgg gtaaaatcct caacaacgtg
      421 aagaagtggc agatcccgcg attcatcaac acggataaag cgcccgccta tggtcgcgcg
      481 cttgctctgc tcaaacgcga aggccggtgc ccgtctgacg ttgaacaccg acagattaag
      541 taccggaaca acgtgattga atgcgatcat ggcaaactga aacggataat cggcgccacg
      601 ctgggattta aatccatgaa gacggcttac gccaccatca aaggtattga ggtgatgcgt
      661 gcactacgca aaggccaggc ctcagcattt tatta
//
LOCUS       DADLTY010000420          692 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.427, whole genome shotgun sequence.
ACCESSION   DADLTY010000420 DADLTY010000000
VERSION     DADLTY010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.427"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(252..674)
                     /locus_tag="JN334_004511"
     CDS             complement(252..674)
                     /locus_tag="JN334_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY7440381.1"
                     /translation="MFNLMLTSKKLTKTAINEALRRMKKAGLSKSELEAFLRDMINGK
                     QKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELLNDAHPK
                     WSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          180 a          193 c          124 g          195 t
ORIGIN      
        1 gacttgaacc agcgacctgg cgattatgag tcgctcgctc tgaccaactg agctaaaggg
       61 ccggaggcag aataataacc atatgtcatc acatctgcaa actcatctga tcaccagcac
      121 gtttaacgtc ctgtgccgtt tttcaggtat aaaaaaaacc gcataaagcg ggctctttca
      181 aatgtccatg tctgctattc gcctcgcggt acagctttgc gaagcgtaca agaattgaag
      241 cagtttttac gtcaaaaagc aataactttt ttctctatac caaaagccat aaccattggt
      301 ttgtacaaaa taaattctgc cacctttagc caatgctcaa tgcgtctttc acaggttctt
      361 aaactccatt tcgggtgtgc atcattcagc agttcagcca ttttgcattt ggtcatcccc
      421 cgcccctcat accgttgccg aaggacgcta atcaatcctg gatgctctgc cagcacctca
      481 cttatgaccc gatcaataca taacgcctct gcatcagtac aatgcgccag ccagctcttt
      541 tgcttaccgt taatcatatc ccgcaaaaaa gcctcaagtt cagacttgct cagacctgct
      601 tttttcatcc tccggagcgc ctcgttaatt gccgtttttg tcagcttttt agaggtcaac
      661 atcaggttaa acatattccc cgtcttaccg cc
//
LOCUS       DADLTY010000421          685 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.428, whole genome shotgun sequence.
ACCESSION   DADLTY010000421 DADLTY010000000
VERSION     DADLTY010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.428"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            213..>685
                     /locus_tag="JN334_004512"
     CDS             213..>685
                     /locus_tag="JN334_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY7440382.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT          187 a          140 c          161 g          197 t
ORIGIN      
        1 ttttccttgc caacaatctt gcaaaaaaaa tgcgattgct aaaacaaaag tgcaatttgc
       61 ggcaatatag ttattccatg accgtacaat cttgcattca gtgtaaataa tcttgtttag
      121 ctctcgctaa acgcggttgt ataaccgcaa tacacgccgt attttatccg gcatatattg
      181 tttttgcttg ctcatggaat agggaggttg tcatgtatca acgctgtttt gataatgcca
      241 gtgaaacgct gtttgtcgcc ggtaaaacgc cacggctttc acgttttgca tttagcgatg
      301 atccaaaatg ggagtctgga catcacgttc atgacaatga aaccgagctg atttacgtca
      361 agaaaggggt tgcaaggttt accatcgatt cttcgttata tgtcgcacat gcggatgaca
      421 ttgtggtgat agaacgcggc aggctgcatg cggtggcctc tgacgttaac gatccggcaa
      481 cgacgtgtac ctgtgcgctg tacggctttc agtttcaggg ggctgaggaa aatcagttac
      541 tgcaaccgca ttcttgtccg gtaattgccg cggggcaggg aaaagaagtc ataaaaacct
      601 tatttaatga gctaagtgtg attttgccgc aaagtaaaaa tagccaaaca tcttcgttat
      661 gggatgcatt agcctataca ttagc
//
LOCUS       DADLTY010000422          683 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.429, whole genome shotgun sequence.
ACCESSION   DADLTY010000422 DADLTY010000000
VERSION     DADLTY010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.429"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            50..683
                     /locus_tag="JN334_004513"
                     /pseudo
     CDS             50..683
                     /locus_tag="JN334_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          205 a          171 c          172 g          135 t
ORIGIN      
        1 cacctgaaca gaggtgatat gctcacctca gaacaacaca ggtgctccaa tgaaaaaaag
       61 aaattttagc gcagagttta aacgcgaatc cgctcaactg gttgttgacc agaaatacac
      121 ggtggcagat gccgccaaag ctatggatgt tggcctttcc acaatgacaa gatgggtcaa
      181 acaactgcgt gatgagcgtc agggcaaaac accaaaagcc tcccccatta ccccggaaca
      241 aattgaaatc cgtgagctca ggaaaaagct acaacgcatt gaaatggaga atgaaatatt
      301 aaaaaaggct accgcgctct tgatgtcaga ctccctgaac agttctcgat aatcgggaaa
      361 ctcagagcgc attatcctgt ggtcacactc tgccatgtgt tcggggttca tcgcagcagc
      421 tacagatact ggaaaaaccg tcctgaaaaa ccagacggca gacgggctgt attacgcagt
      481 caggtacttg agctacatgg catcagccac ggttcggccg gagcaagaag catcgccaca
      541 atggcaaccc ggagaggcta ccagatggga cgctggcttg ctggcaggct catgaaagag
      601 ctggggctgg tcagctgtca gcagccgact caccggtata aacgtggtgg tcatgaacat
      661 gttgctatcc ctaactacct tga
//
LOCUS       DADLTY010000423          669 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.430, whole genome shotgun sequence.
ACCESSION   DADLTY010000423 DADLTY010000000
VERSION     DADLTY010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.430"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(180..653)
                     /locus_tag="JN334_004514"
     CDS             complement(180..653)
                     /locus_tag="JN334_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HAY7440383.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
BASE COUNT          173 a          160 c          142 g          194 t
ORIGIN      
        1 tgcgaatatg tgagttttga cctaatgagc cacaaaaact cattttcgcc cacgataatt
       61 tttctacttc ctttctttgg aatgctcatt tcaatattca cataacgtcg ctatcaatcg
      121 cggaaaaggc gtagtgcgcg aagcgaacga agctttttgc cgtcgattgc atagctttgt
      181 taaccctttt tccaaatttg atagcaatag ttaatgtttg aagtaaagtg ttgctcaaaa
      241 acaacttcga aggtatttgg aatactcggg aagaaaacat ccccctctgg ctcgatgtcg
      301 atcgtcgata agtggagcgt agaggccatg ggtaatgttt ctcggtaaat ttctccgcca
      361 ccagacacta taacgtgacc ggtgaattca gctagcctgt ccatggcctc ttcgattgac
      421 tgaaatacaa ctacattgtc atcatttgat gtccaacctg agcgggtaac gaccgcgtat
      481 ttcctattgg ggagtgcgcc catagattca aacgtcttgc gacccaccag aagccactga
      541 ttgtaggtca atgctttaaa aagtagctgc tcccctttcg cggaccaggg tatgtctgga
      601 ccgcaaccaa tcacgccgtt tttcgctttc gcagccatca atgatacttt caaggttctc
      661 atcctgggt
//
LOCUS       DADLTY010000424          668 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.431, whole genome shotgun sequence.
ACCESSION   DADLTY010000424 DADLTY010000000
VERSION     DADLTY010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.431"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..>668
                     /locus_tag="JN334_004515"
     CDS             <1..>668
                     /locus_tag="JN334_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HAY7440384.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKR"
BASE COUNT          133 a          230 c          177 g          128 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
      661 tcaaacgg
//
LOCUS       DADLTY010000425          666 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.432, whole genome shotgun sequence.
ACCESSION   DADLTY010000425 DADLTY010000000
VERSION     DADLTY010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.432"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            82..>666
                     /locus_tag="JN334_004516"
     CDS             82..>666
                     /locus_tag="JN334_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7440385.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITR"
BASE COUNT          139 a          180 c          199 g          148 t
ORIGIN      
        1 tgccggtatc agcgccggcg gtatgtactg cccggagtgt ggcacggttc actggccgga
       61 cggagtcgcc ccgcccttct gatgctcccc cgttttgccg atatttttca gcagggaaac
      121 cgctggctta actggctgga gaaacaaccg gaaggggcag tccgtccggt ggtaattgaa
      181 tcagtgacaa aaatcatggc ctgcgggacc acactgatgg gctacacgca gtggtgttgt
      241 tcatcaccgg actgttgcca tacaaaaaag gtctgcttcc ggtgtaaaag ccgctcctgc
      301 ccgcactgcg gggtgaaggc tggcgcacag tggatacagt atctgctgag cctggtcccc
      361 tactgcccgt ggcagcatat tgtgttcaca cttccctgcc agtactggtc cctggtgttc
      421 cacaaccggt ggttactggc agagatgagc cgcattgcag cggatgtgat actggaaatc
      481 tgccatcagg cagatgtgga gccggggata ttcacggtga tccacacatg ggggcgtgac
      541 cagcagtggc atccgcatat ccatttatcg acaactgccg gtggtgtgac gtcgggccac
      601 acctggaaaa atcttcattt ttacgcccgt aaggtgatga gcatgtggcg ctaccggata
      661 acacgg
//
LOCUS       DADLTY010000426          656 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.433, whole genome shotgun sequence.
ACCESSION   DADLTY010000426 DADLTY010000000
VERSION     DADLTY010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.433"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(151..648)
                     /locus_tag="JN334_004517"
     CDS             complement(151..648)
                     /locus_tag="JN334_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY7440386.1"
                     /translation="MTTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDLKE
                     LRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDPST
                     PVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKLVE
                     RLMQL"
BASE COUNT          173 a          160 c          144 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc gtaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaa
//
LOCUS       DADLTY010000427          636 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.434, whole genome shotgun sequence.
ACCESSION   DADLTY010000427 DADLTY010000000
VERSION     DADLTY010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.434"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            88..153
                     /locus_tag="JN334_004518"
                     /pseudo
     CDS             88..153
                     /locus_tag="JN334_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="JN334_004519"
     CDS             381..629
                     /locus_tag="JN334_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY7440387.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          202 a          131 c          147 g          156 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacac
//
LOCUS       DADLTY010000428          635 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.435, whole genome shotgun sequence.
ACCESSION   DADLTY010000428 DADLTY010000000
VERSION     DADLTY010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.435"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            18..491
                     /locus_tag="JN334_004520"
     CDS             18..491
                     /locus_tag="JN334_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7440388.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          210 a          113 c          127 g          185 t
ORIGIN      
        1 gagatgaacc ctatgcaatg tcctggttaa tatctcagtg tgctcatcag tgcactgata
       61 ataaaaagac ggagacagat gccatttacg ataaagttcg gagttcttat ttgctttcat
      121 gtattctgaa aaaaaacaaa aatgttgggc ttattcttca tgcaccatca tttgtttctg
      181 tgtctgaaaa aatcgccaga attgtgatgg caaattactc ccgtaactgg agtaattccg
      241 agttggccag tgcggtttta atgagtgaat cttctttaaa aagaagaatg tacaaagagg
      301 tcggttctat aagtacattt gtacataaaa taaagcttac agaagctatc aggaaattac
      361 gaaggacaaa tactcccatt tctgtcatat catctgagtt aggttacagt tcaccatcgt
      421 atttttctaa agtcttcttt aaatatttaa agacataccc tcaaaacata aggaagaaaa
      481 atggtcggta atgtcaccaa ttaggaggac ttgcagagcc gcttttttac gatactgaga
      541 tcaaacagac ataatctatg cggagattta cagcagggca tggttcacaa atctgacagc
      601 gatgaattat cggcactgcg ggcagaaaat gctcg
//
LOCUS       DADLTY010000429          624 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.436, whole genome shotgun sequence.
ACCESSION   DADLTY010000429 DADLTY010000000
VERSION     DADLTY010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.436"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..>624)
                     /locus_tag="JN334_004521"
     CDS             complement(<1..>624)
                     /locus_tag="JN334_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7440389.1"
                     /translation="VWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPE
                     KQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKK
                     IYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
BASE COUNT          140 a          173 c          140 g          171 t
ORIGIN      
        1 gacacttccg agccgttgat aatactggtt ttcatattct gtcggtgaca tctgttcgct
       61 agaaccatgc cgacgcttac tgttataaaa catttcgatg taatcaaaaa tatcactgcg
      121 ggcttcttcc cgcgttccgt agatcttttt ctttatccgt tcacgtttca acaactggaa
      181 aaaactttct gcaaccgcat tatcatggca gttaccgcga cggctcatgc taccctccag
      241 gccgtgtgat ttcaggaacg actgccactc atggcttgtg tactgactgc cctgatccga
      301 atgaaccagc acctgttttt cgggattacg ccgccataca gccatcagca gtgcgttcag
      361 gacaatgtcc tttgtcatcc gggattgcat ggaccagccg ataattttgc gtgagaacag
      421 atcaacaaca acggcaagat acagccagcc ttcgtgggtc ctgatgtagg ttatgtccgt
      481 tacccaacgc tcatcaggag catccggatt gaactgtcgc tggagcctgt tgggtgacac
      541 gatactggcc tcgcctttac gtgcccgcgg gcttcggtat ccgacctgag cctttattcc
      601 gacacgtttc atcagtctcc agac
//
LOCUS       DADLTY010000430          622 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.437, whole genome shotgun sequence.
ACCESSION   DADLTY010000430 DADLTY010000000
VERSION     DADLTY010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.437"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..251)
                     /locus_tag="JN334_004522"
     CDS             complement(<1..251)
                     /locus_tag="JN334_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY7440390.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
     gene            complement(272..>622)
                     /locus_tag="JN334_004523"
     CDS             complement(272..>622)
                     /locus_tag="JN334_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7440391.1"
                     /translation="TVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRH
                     RKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNRR
                     LSESVPCMPHKKAG"
BASE COUNT          140 a          145 c          141 g          196 t
ORIGIN      
        1 ctttgcaggt aagtcagtaa tgtttcttgc tgatcgcgcc agacgttctg cacatcaaca
       61 ttttcattac tgagcagtat tttaactttg ctgacctgca cgccgttgtc gatccagcgt
      121 ttgatctcgc ggatccggtc aatatcggca tcgttgaaca gccgatgacc gccgtctgtc
      181 cgttgcggtt tcagcaatcc gtaacgcctc tgccacgcgc gtaacgtgac agggattaaa
      241 ggtaaaccaa ttgattttgc aagtttttat tttaccccgc tttcttatgg ggcatgcatg
      301 ggacactttc agatagtctt ctgttaagaa gttctatctg ttcgtgattg ttgtctttca
      361 tccatgctcc gtaaacattg aataccattt gtgcgtttgt gtggcccatc tggcttgcga
      421 taaaactagg attagctcct gcggcaagtg accagcatgc ataggtatgc ctggattgat
      481 acgattttct gtgtctcaga cctgcgcgtt ttaagatact tgtccatgac tccctgatgg
      541 agtcaacctt atagtgcggt ccggaaaact gccgctgttt tattacctga ggactaaaaa
      601 caaaagtgca tttatgcaca gt
//
LOCUS       DADLTY010000431          602 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.438, whole genome shotgun sequence.
ACCESSION   DADLTY010000431 DADLTY010000000
VERSION     DADLTY010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.438"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..186)
                     /locus_tag="JN334_004524"
     CDS             complement(<1..186)
                     /locus_tag="JN334_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014462364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440392.1"
                     /translation="MPFHRPTKWVQYISAAPALSQQAVDQEWSYMDFLEHLLHEEKLA
                     RHQRKQAMYTRMAAFPAV"
     gene            197..>602
                     /locus_tag="JN334_004525"
     CDS             197..>602
                     /locus_tag="JN334_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7440393.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASA"
BASE COUNT          155 a          138 c          151 g          158 t
ORIGIN      
        1 caccgccggg aaggctgcca ttcgggtata catcgcctgt ttacgttgat gacgtgccag
       61 tttttcttca tgaagcagat gctccaggaa gtccatataa ctccattcct ggtctactgc
      121 ctgttgtgac agcgcaggcg ctgcgcttat gtactgcacc cattttgttg gacgatgaaa
      181 tggaatagcc cctgatatgt caaagccaaa ataccctttt gaaaagcgcc ttgaagtcgt
      241 gaatcactac ttcactactg atgatggtta caggatcatc tcggcacgtt ttggtgtccc
      301 ccgaacccag gtcaggacat gggttgccct ctatgaaaaa catggagaaa aaggcttaat
      361 tcccaaacct aaaggtgtta gtgctgatcc agagttgcgt attaaggtcg tgaaagctgt
      421 gattgagcag cacatgtccc ttaatcaggc tgctgctcac tttatgcttg ctggtagtgg
      481 ttctgtagcc aggtggctga aggtttatga agagcgcgga gaagctggtt tacgcgcgct
      541 caagattggc accaaaagaa acattgcaat atcagttgat ccagaaaaag cggcatcagc
      601 at
//
LOCUS       DADLTY010000432          602 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.439, whole genome shotgun sequence.
ACCESSION   DADLTY010000432 DADLTY010000000
VERSION     DADLTY010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.439"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(1..602)
                     /locus_tag="JN334_004526"
                     /pseudo
     CDS             complement(1..602)
                     /locus_tag="JN334_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          130 a          168 c          152 g          152 t
ORIGIN      
        1 gggcactgaa atccccaccg gtcccagtcg tgagcctctg tatgactgcc ccctgacaag
       61 tctgatgact ttatcaccgt cagcttctcc ctggtacgca gcaataatca gcccgccaat
      121 gtcagggtct ccccatctgt tgcggacaga gctcgccacg attctgtaaa taatatcttc
      181 tgtggttata tttattttca cccagtcagt ctggatatgg gccagtagcg gtagcgggtg
      241 tgataaatgg ggccgttaat gccgtaaaaa gtaagggatt tggctctgta ccgcggttct
      301 gttgtctcag ggcgtgcatc agtccaccgg atgctgagca ccccttcaaa ccgtgtgtcg
      361 ggtatgatga tgccgtaggg gccagcaacg gaatattcac ctggcagcgc attccttacc
      421 caggccagga aatcactctt agtgtcaaaa cggataacat cttcaggcag aaaagcacac
      481 ccaaagccga atgcgccggg tatcgccaga cggccttttg tccggtcgta aatgtcgctc
      541 tgtgcttcca tcgtggccgc acttttcagc cccagattat cccgggactt ctgttgtgcc
      601 tt
//
LOCUS       DADLTY010000433          594 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.440, whole genome shotgun sequence.
ACCESSION   DADLTY010000433 DADLTY010000000
VERSION     DADLTY010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.440"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..312
                     /locus_tag="JN334_004527"
     CDS             <1..312
                     /locus_tag="JN334_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HAY7440394.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
     gene            315..>594
                     /locus_tag="JN334_004528"
     CDS             315..>594
                     /locus_tag="JN334_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAY7440395.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGL"
BASE COUNT          111 a          150 c          161 g          172 t
ORIGIN      
        1 gtggctttta tggataacgc ccacgttcag gtcacctttc tggtggagaa actctccccc
       61 gcatggcaac gacgagtcgc tctggttacc cattccttaa tcttatttat ttgtggcgca
      121 ctggcctggg gcgcaacgct aaaaacaatt caggactgga gcgattattc accgatcctc
      181 ggtttgccca tcggcctgat gtatgccgct tgtctgccca ccagcctcgt tatcgcattc
      241 tttgaactgc gtcatttata tcaactcatc acgcgtagca attctctaac gtcgccaccg
      301 caaggagctt aatcatggct gtgctgattt ttctgggctg tctgttgggt ggtattgcta
      361 tcggtttgcc tattgcctgg gcactgttgt tgtgcggggc ggcgttaatg ttctggctgg
      421 acatgtttga tgtccagatc atggcacaaa cgctggtgaa cggagccgat agcttctccc
      481 tcctggcgat tccgttcttt gtgctggcgg gtgaaatcat gaatgcgggc ggcttgttaa
      541 aacgcattgt tgacctgccg atgaaactgg tggggcataa accaggcggg ctgg
//
LOCUS       DADLTY010000434          592 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.441, whole genome shotgun sequence.
ACCESSION   DADLTY010000434 DADLTY010000000
VERSION     DADLTY010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.441"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            43..>592
                     /locus_tag="JN334_004529"
     CDS             43..>592
                     /locus_tag="JN334_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7440396.1"
                     /translation="MTVAQCLDYWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPA
                     SSITVKQWVDLLTEEEKDNPRRARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGS
                     RAEVGDRVLSYHELAKIWLAIERSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLL
                     DNVWTVPKEHSKMGNVIRRPIFE"
BASE COUNT          161 a          142 c          154 g          135 t
ORIGIN      
        1 acggatccac gcacctattt tgaagagaag gtggagaact ccatgacggt cgcccagtgt
       61 ctcgactact ggttcgacaa ctacgtctct acaactctca gagaaaagac ccaggcactt
      121 tatcgatcaa cggttatgaa gcgcatgcat gacgccttcc ctaatcgtcc ggcatcttct
      181 atcacggtta agcaatgggt tgacctgctt accgaagaag aaaaagataa tccacgccga
      241 gcaaggcagg tgctaagtca actaagatca gcaattagtt ggtgcatgcg gcgtcagttg
      301 atagatagtt gcgcaattat gagcatccag ccaagggact tcggctcccg cgctgaggta
      361 ggggatcggg tactgtcgta tcacgaactg gctaagattt ggcttgctat tgaaagaagc
      421 cgtgcgtcta cgtcaaataa gctccttcat cagatgctta tgctgtgggg ggcgaggctc
      481 tcagagctta ggctggcaac aaagacagaa tttgacctgc tggacaacgt atggaccgta
      541 ccgaaagagc atagcaagat gggtaatgtt atccgccgtc caatcttcga ac
//
LOCUS       DADLTY010000435          587 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.442, whole genome shotgun sequence.
ACCESSION   DADLTY010000435 DADLTY010000000
VERSION     DADLTY010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.442"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            57..554
                     /locus_tag="JN334_004530"
                     /pseudo
     CDS             57..554
                     /locus_tag="JN334_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          142 a          144 c          181 g          120 t
ORIGIN      
        1 gccagccccg tcccgcccga tagagcgcgg catagcgggc cccggattac ttgcgtgcga
       61 cggcagaaga agccctgaag cgaatcagtg aactgtacgc catcgaggat gaaatacggg
      121 gattaccaga gtcagagtgt cttgcagtca ggcagcagcg aagcaaagcg ttactgacgt
      181 cgctgcatga atggatggtg gagaagaatg gcacgctgtc gaaaaaatcc agactgggcg
      241 aagcattcag ctatgtactg aatcagtggg acgccctctg ttattacagt gatgacggtc
      301 tggcggaggc ggacaataac acagcggaaa gagcgcttcg tgcagtctgt ctcggaaaga
      361 aaaattacgt gttcttcggt agcgatcacg gcggcgagcg tggtgcactg ctgtacgggc
      421 tgatcggcac ctgccgtctg aacggtatcg atccggaagc gtatctgcgc catattctga
      481 gcgtactgcc ggaatggcct tccaaccgag ttgacgacct cctgccatgg aaggtggtgc
      541 taccatcagg ataatcgtca atacggtgtt cggtgaacgt ttacttg
//
LOCUS       DADLTY010000436          583 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.443, whole genome shotgun sequence.
ACCESSION   DADLTY010000436 DADLTY010000000
VERSION     DADLTY010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.443"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..>583
                     /locus_tag="JN334_004531"
     CDS             <1..>583
                     /locus_tag="JN334_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HAY7440397.1"
                     /translation="SRNTVKRYLQAKSEPPKYTPRPAVASLLDEYRDYIRQRIADAHP
                     YKIPATVIAREIRDQGYRGGMTILRAFIRSLSVPQEQEPAVRFETEPGRQMQVDWGTM
                     RNGRSPLHVFVAVPGYSRMLYIEFTDNMRYDTLETCHRNAFRFFGGVPREVLYDNMKT
                     VVLQRDAYQTGQHRFHPSLWQFGKEMGFSPRLCR"
BASE COUNT          127 a          158 c          150 g          148 t
ORIGIN      
        1 tctcccgcaa taccgttaaa cgttatttgc aggcaaaatc tgagccgcca aaatatacgc
       61 cgcgacctgc tgttgcttca ctcctggatg aataccggga ttatattcgt caacgcatcg
      121 ccgatgctca tccttacaaa atcccggcaa cggtaatcgc tcgcgagatc agagaccagg
      181 gatatcgtgg cggaatgacc attctcaggg cattcattcg ttctctctcg gttcctcagg
      241 agcaggagcc tgccgttcgg ttcgaaactg aacccggacg acagatgcag gttgactggg
      301 gcactatgcg taatggtcgc tcaccgcttc acgtgttcgt tgctgttccc ggatacagcc
      361 gaatgttgta catcgaattc actgacaata tgcgttatga cacgctggag acctgccatc
      421 gtaatgcgtt ccgcttcttt ggtggtgtgc cgcgcgaagt gttgtatgac aatatgaaaa
      481 ctgtggttct gcaacgtgac gcatatcaga ccggtcagca ccggttccat ccttcgctgt
      541 ggcagttcgg caaggagatg ggcttctctc cccgactgtg tcg
//
LOCUS       DADLTY010000437          577 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.444, whole genome shotgun sequence.
ACCESSION   DADLTY010000437 DADLTY010000000
VERSION     DADLTY010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.444"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..98
                     /locus_tag="JN334_004532"
     CDS             <1..98
                     /locus_tag="JN334_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAY7440398.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            99..512
                     /locus_tag="JN334_004533"
     CDS             99..512
                     /locus_tag="JN334_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440399.1"
                     /translation="MKLAIRLQLPLAFASKERSMEIKPEDELSNIVLFPIKEDDPRNQ
                     VNFLYKPSERPYCHHASVRVDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRL
                     LRQEEQERRRNIEKLIQIERNAKARIRRATKSRTE"
BASE COUNT          183 a          110 c          149 g          135 t
ORIGIN      
        1 ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg
       61 ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc
      121 aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt
      181 taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag gttaattttc
      241 tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag
      301 agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct
      361 ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac
      421 aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga
      481 tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat
      541 ccttaaccgg agggatttct gcaccctcaa atcatca
//
LOCUS       DADLTY010000438          571 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.445, whole genome shotgun sequence.
ACCESSION   DADLTY010000438 DADLTY010000000
VERSION     DADLTY010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.445"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(47..571)
                     /locus_tag="JN334_004534"
                     /pseudo
     CDS             complement(47..571)
                     /locus_tag="JN334_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          160 a          135 c          116 g          160 t
ORIGIN      
        1 ccatcttaaa tgactctgag gtgtctgtta aacccgtggc gattcaggtc tgcatagcat
       61 tatcaacatt cggatgtgca actacagcta atgcaacatc atcccagctt gcaatagtgc
      121 tatcagcaac accatctccg tcagtatcta cggtgacaaa gttagattta ccactataat
      181 cgctggcatt acctgtatta ccgaaccaac cagtagtacc ttcatcagtc catgaaccag
      241 aggttacggt agaatcttta acagtgatgg tgttattaaa cacttcgctg ctgtttacgc
      301 cagtgctgac agtgttgtcg tcaatatctt cccactcata accctgagtc aacgtaatac
      361 ccgcaacgtg agagttatta gtgatggaca gatcaacttc ctgatccaga gtaatagccg
      421 taccaatgtt gtaaacgtct acaacatgag tatcagcggc attattgtaa gtgccgttat
      481 atgtgtagtg ttcgtagttg tcgtcgatcg ttgaattatc aacagtcagc gccagacggt
      541 catggtagta atcatccgca cgatcagcac a
//
LOCUS       DADLTY010000439          566 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.446, whole genome shotgun sequence.
ACCESSION   DADLTY010000439 DADLTY010000000
VERSION     DADLTY010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.446"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..297
                     /locus_tag="JN334_004535"
     CDS             <1..297
                     /locus_tag="JN334_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HAY7440400.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="JN334_004536"
     CDS             complement(405..>566)
                     /locus_tag="JN334_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440401.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DADLTY010000440          553 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.447, whole genome shotgun sequence.
ACCESSION   DADLTY010000440 DADLTY010000000
VERSION     DADLTY010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.447"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..>553
                     /locus_tag="JN334_004537"
     CDS             <1..>553
                     /locus_tag="JN334_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HAY7440402.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETV"
BASE COUNT          163 a          135 c          147 g          108 t
ORIGIN      
        1 tgcgccaact ctgcgtatcc gtgacccgca taaagatgaa aagatggcag caatccataa
       61 agcactggac gaatgcagcg cagagcatcc ggtcttttat gaagatgaag tggatatcca
      121 tcttcatccc aaaatcggtg cggactggca actgcgcgga cagcaaaaac gggtggtcac
      181 gccgggacag aatgaaaaat attatctggc cggagcgctg cacagcggga caggtaaagt
      241 cagctatgtg ggcggcaaca gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct
      301 taaagcgaca taccgtcgtg cgaaaaccat cacgctgatc gtggacaact acattatcca
      361 caaaagccgg gaaacacaga gctggctgaa ggagaacccg aagttcaggg gcatttatca
      421 gccggtttac tcgccatggg tgaatcatgt tgaacggcta tggcaggcac ttcacgacac
      481 aataacgcgt aatcatcagt gccgctcaat gtggcaactg ttgaaaaaag ttcgccattt
      541 tatggaaacc gtc
//
LOCUS       DADLTY010000441          550 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.448, whole genome shotgun sequence.
ACCESSION   DADLTY010000441 DADLTY010000000
VERSION     DADLTY010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.448"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..>550)
                     /locus_tag="JN334_004538"
     CDS             complement(<1..>550)
                     /locus_tag="JN334_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440403.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIK"
BASE COUNT          133 a          117 c          135 g          165 t
ORIGIN      
        1 atttaatatc aatctgttga aggtgtatta ttacatcagt agcatattcc agactgcctt
       61 cctggacgat ctgatccata acatcctcac gcactttata tcttgtgcat ttcatttcaa
      121 gtgccgtgag tacagccatt actggtgatg gtagctgtgt tgttttcagg ccgtcgatct
      181 gactgatcgc ttgggcgacg gtgttttgcc attctaaaga acaggtgctg aagtcaaatg
      241 tggctgtttt attatcccgt tgcgacacgc tacaggctaa gcgacgtaat tgggtcaatt
      301 tgtctgcttg ctgaacagga actccgggaa agcggcgatg acttaacgtg gtgtgtacaa
      361 cttcgggcgg aaaaacgaca gcaatatctt tcgccagcca aggtaaaaca gcatctgtat
      421 ttttgtcagc cgcacgggtt tctgctacga aagagggttg ttcctgaata atggtggata
      481 agtccgctac cttgagagag tccgttaccg tttgggaagg ttgcaaagaa ttatctttcg
      541 cttgctccac
//
LOCUS       DADLTY010000442          515 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.449, whole genome shotgun sequence.
ACCESSION   DADLTY010000442 DADLTY010000000
VERSION     DADLTY010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.449"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            101..>515
                     /locus_tag="JN334_004539"
     CDS             101..>515
                     /locus_tag="JN334_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HAY7440404.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPS"
BASE COUNT          128 a          142 c          133 g          112 t
ORIGIN      
        1 atagctgcgc ggaatagtag atccctttga gggaacttag cccggattgt gcgatctgat
       61 caatcgccaa atcaaaacaa atcaccaacc ggactgagca atgccgatca tagcaccaat
      121 ttcccgtgac gaacgacgcc tgatgcagaa agccatccat aaaacacacg ataaaaatta
      181 tgcccgcaga ctgactgcca tgctgatgct gcaccggggc gaccgtgtca gcgacgttgc
      241 cagaacgctc tgctgcgccc gttcctctgt tggacgctgg attaactggt tcacgcagtc
      301 gggtgttgag ggactgaaat cattacctgc cgggcgtgcc cgtcgctggc cgtttgagca
      361 tatctgcaca ctgttacgtg agctggtaaa acattctccc ggcgactttg gctaccagcg
      421 ttcacgctgg agtacagaac tgctggcaat aaaaatcaat gagataaccg gctgccagtt
      481 aaatgccgga accgttcgcc gctggttgcc gtctg
//
LOCUS       DADLTY010000443          504 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.450, whole genome shotgun sequence.
ACCESSION   DADLTY010000443 DADLTY010000000
VERSION     DADLTY010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.450"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            131..504
                     /locus_tag="JN334_004540"
                     /pseudo
     CDS             131..504
                     /locus_tag="JN334_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT          119 a          108 c          169 g          108 t
ORIGIN      
        1 ctgccggtat caacgccgac ggcatgtatt gccctgaatg cggtacggtt cactggccgg
       61 atggcgttat tccgcctttc tgatgcttcc ccgttttgcc gatatttttc agcagggaaa
      121 ccgatggctt aactggctgg agaagcagcc ggtgcagatg agccgcctgg aacattatgc
      181 cggtcaggat gaaattggcc tgcgttacaa cagccaccgg acaaaacgag aagaaaacct
      241 tgtgatgagt ggcgacgagt ttatggaaag gttctcctgg catgtggcgg ataaggggtt
      301 ccggatggtg atacggggtc ctgagtccgg tgaagcggcg attactggaa gatgtggtgt
      361 acgccataac ggagacagtg agaaaaacgg cgaggcaaat cacaaggaga gggatgtatc
      421 agcagtgtct gaaggttgac ccgctgaagt gcgagctgcg cggaggtcag atgcggttta
      481 cggggctgaa gaggggttac cgtc
//
LOCUS       DADLTY010000444          493 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.451, whole genome shotgun sequence.
ACCESSION   DADLTY010000444 DADLTY010000000
VERSION     DADLTY010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.451"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            1..493
                     /locus_tag="JN334_004541"
                     /pseudo
     CDS             1..493
                     /locus_tag="JN334_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          121 a          125 c          136 g          111 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggt
//
LOCUS       DADLTY010000445          484 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.452, whole genome shotgun sequence.
ACCESSION   DADLTY010000445 DADLTY010000000
VERSION     DADLTY010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.452"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..>484
                     /locus_tag="JN334_004542"
     CDS             <1..>484
                     /locus_tag="JN334_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7440405.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     E"
BASE COUNT          109 a          107 c          123 g          145 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cgaa
//
LOCUS       DADLTY010000446          483 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.453, whole genome shotgun sequence.
ACCESSION   DADLTY010000446 DADLTY010000000
VERSION     DADLTY010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.453"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            32..>483
                     /locus_tag="JN334_004543"
     CDS             32..>483
                     /locus_tag="JN334_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HAY7440406.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFK"
BASE COUNT          155 a           97 c          105 g          126 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 aga
//
LOCUS       DADLTY010000447          479 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.454, whole genome shotgun sequence.
ACCESSION   DADLTY010000447 DADLTY010000000
VERSION     DADLTY010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.454"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..250)
                     /locus_tag="JN334_004544"
     CDS             complement(<1..250)
                     /locus_tag="JN334_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440407.1"
                     /translation="MDITVCTYKPGGIVEKIKKLFSSKYAVIRRDDLSVIVEMDYFPE
                     TPKSIMYRNGRKAIFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          135 a           98 c           89 g          157 t
ORIGIN      
        1 aacaggatgc tctgactcgc aattcatcca gcagtttatc atttcccata atgtcactta
       61 ccctcatcgg taaaaaaatt gcctttcgac cattacgata cattattgat tttggggttt
      121 cagggaagta atccatttcg actataactg acaggtcatc acgacgtatg actgcgtatt
      181 tgctactaaa tagtttcttt attttttcca cgatgccccc aggtttataa gtacaaacgg
      241 ttatatccac atagagacaa aaatattaat ctgataaata tttatttcac gccgtatatt
      301 tgattgttta atgtgcaagt acaatgactt ttattttttg ttgtgtatat aatcaaatat
      361 atggttattt ttcaccctgc gtattcagca cgcaacaaaa aacccgccga agcgggttta
      421 gtgcgggtgc gttgaggatg cctgacacat gagaggtggc gagggatttc tcccccgcc
//
LOCUS       DADLTY010000448          472 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.455, whole genome shotgun sequence.
ACCESSION   DADLTY010000448 DADLTY010000000
VERSION     DADLTY010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.455"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..>472
                     /locus_tag="JN334_004545"
     CDS             <1..>472
                     /locus_tag="JN334_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7440408.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DADLTY010000449          467 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.456, whole genome shotgun sequence.
ACCESSION   DADLTY010000449 DADLTY010000000
VERSION     DADLTY010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.456"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            39..>467
                     /locus_tag="JN334_004546"
     CDS             39..>467
                     /locus_tag="JN334_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440409.1"
                     /translation="MENFIDTLENWRDQKQIISVICKDGIKIDDSIITSFKASKDVGI
                     SNGLRIQLTFQEINFKAIVGQTDVSAATGRTSTTNDGGATSKKNTGNTTTSLGSPMLT
                     CKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASAN"
BASE COUNT          148 a           96 c          102 g          121 t
ORIGIN      
        1 aatccggtag tacccttatc tcacgagata ataagctggt ggagaacttc atcgacactc
       61 ttgaaaactg gcgtgaccag aagcagatta tttcagttat ctgtaaagat ggtattaaga
      121 ttgacgactc cattattaca agctttaaag cctctaaaga tgttggaatt tcaaacggac
      181 tgagaatcca gctaactttt caggaaatta acttcaaagc tattgtaggg caaactgatg
      241 tttcagcagc cactggacgg acttctacca cgaatgatgg cggtgctacc agtaaaaaga
      301 acactggcaa cactacgaca agtcttggaa gccctatgct gacctgtaaa gagcttttta
      361 gctattcggc aagtgagtta tcagatgaag cgttaaaagc ccgtgtaacg tgctctaaga
      421 gcgttagcgt taaaaatggt gagagcacct tcacagcctc agctaat
//
LOCUS       DADLTY010000450          463 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.457, whole genome shotgun sequence.
ACCESSION   DADLTY010000450 DADLTY010000000
VERSION     DADLTY010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.457"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..>463
                     /locus_tag="JN334_004547"
     CDS             <1..>463
                     /locus_tag="JN334_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7440410.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGC"
BASE COUNT          117 a          109 c          142 g           95 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcc
//
LOCUS       DADLTY010000451          459 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.458, whole genome shotgun sequence.
ACCESSION   DADLTY010000451 DADLTY010000000
VERSION     DADLTY010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.458"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..>459)
                     /locus_tag="JN334_004548"
     CDS             complement(<1..>459)
                     /locus_tag="JN334_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HAY7440411.1"
                     /translation="LSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLM
                     FNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVRE
                     KLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          106 a          117 c           90 g          146 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaa
//
LOCUS       DADLTY010000452          450 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.459, whole genome shotgun sequence.
ACCESSION   DADLTY010000452 DADLTY010000000
VERSION     DADLTY010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.459"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..268
                     /locus_tag="JN334_004549"
     CDS             <1..268
                     /locus_tag="JN334_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAY7440412.1"
                     /translation="NLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGNRAEMSPEAAGI
                     AACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            269..>450
                     /locus_tag="JN334_004550"
     CDS             269..>450
                     /locus_tag="JN334_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440413.1"
                     /translation="MVAGTMQQLSFLPGEMTTRERSLILRALKTLDRHLHEPGVAFTS
                     THAAREWLILNMAGLER"
BASE COUNT          104 a          126 c          128 g           92 t
ORIGIN      
        1 gaatctgtac accctgaaca acggcggggc atttatggca cccgaaccgg atgacgatga
       61 tgacgaaaca tgggtactgt tcaatgccat gaacggtaac cgcgctgaaa tgagtccgga
      121 agctgccggc attgctgcct gtctgatgac gtacagtcat catgcctgtc gtacggagtg
      181 ttatgccatg acggtccatt attaccggct gcgggattac gccctgcagc atccggaatg
      241 cagcgccatt atgcgcatca tcgactgaat ggtggcaggg acaatgcagc agctttcctt
      301 tctgcccgga gaaatgacaa ccagagagcg cagcctcatt ctgcgggccc tgaaaaccct
      361 ggaccgccat cttcatgaac ccggcgtggc cttcacctcc acgcatgcgg cacgggaatg
      421 gctgattctg aacatggcgg gactggagcg
//
LOCUS       DADLTY010000453          444 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.460, whole genome shotgun sequence.
ACCESSION   DADLTY010000453 DADLTY010000000
VERSION     DADLTY010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.460"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..379)
                     /locus_tag="JN334_004551"
     CDS             complement(<1..379)
                     /locus_tag="JN334_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440414.1"
                     /translation="MNVVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFG
                     GMVRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEF
                     DIWNLNETWAFQEKLIFCEDESSL"
BASE COUNT          134 a          106 c           95 g          109 t
ORIGIN      
        1 acagacttga ttcatcctca caaaatataa gtttttcctg gaatgcccat gtctcattga
       61 ggttccagat atcaaattcg aagtcgtgat aacgaaaacg gatcccacca aacttattaa
      121 aacgcagttg tttgacgggc agttcagcca atgtctggaa gagttcctca cgggagcgac
      181 cgatgacgat atcaaggtct gaagagaatc catcgacgcc atacaagcct aagtcacgca
      241 ccatcccgcc aaaaatcctg gccggacagg aacaggccag agtatcaagt aatactctgg
      301 ctttcttatg ctcatcacta agcgtaaaaa aatcggcgat acgctgttca agcagttggg
      361 tgtttgagac cacattcata cagtgatttt agctcatcgt gaaggcgcat taaataacta
      421 tccgtcatgc ctgagattgc gtcg
//
LOCUS       DADLTY010000454          443 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.461, whole genome shotgun sequence.
ACCESSION   DADLTY010000454 DADLTY010000000
VERSION     DADLTY010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.461"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            213..>443
                     /locus_tag="JN334_004552"
     CDS             213..>443
                     /locus_tag="JN334_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY7440415.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          141 a          105 c           61 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gga
//
LOCUS       DADLTY010000455          437 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.462, whole genome shotgun sequence.
ACCESSION   DADLTY010000455 DADLTY010000000
VERSION     DADLTY010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.462"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..163
                     /locus_tag="JN334_004553"
     CDS             <1..163
                     /locus_tag="JN334_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7440416.1"
                     /translation="PLLPVHLKAGLKPDTMRNVFRCHIPRYEAIIARYLDMEPAQIWP
                     SRYMEKTSC"
     gene            157..375
                     /locus_tag="JN334_004554"
                     /pseudo
     CDS             157..375
                     /locus_tag="JN334_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          127 a          102 c          100 g          108 t
ORIGIN      
        1 tccgctattg ccagtacacc tcaaggcagg cttaaagcct gacactatgc gtaacgtatt
       61 tcgctgccat ataccacgat acgaagcaat cattgctaga tatttggata tggaaccagc
      121 ccagatttgg ccttctcggt atatggagaa aacatcatgt taacccattg ggtaacagcg
      181 caagaatgcg taggaatgtc atgctttcct aaacatgagg tgagtattcg ccgcaatctt
      241 gaaaagcttg cagccaatca tccagaacta cgtcgcaagc gtacgggttc aaaggctttg
      301 gaatttcata tatcagttct accagcggca gcacgtgctg agttgttatt aaaatctgag
      361 caaatagaaa caagctaggg gtattttgaa atcgcccgcc ccacgctgga agcgcacgat
      421 tacgatcgtg aggcact
//
LOCUS       DADLTY010000456          434 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.463, whole genome shotgun sequence.
ACCESSION   DADLTY010000456 DADLTY010000000
VERSION     DADLTY010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.463"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            35..434
                     /locus_tag="JN334_004555"
                     /pseudo
     CDS             35..434
                     /locus_tag="JN334_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DADLTY010000457          420 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.464, whole genome shotgun sequence.
ACCESSION   DADLTY010000457 DADLTY010000000
VERSION     DADLTY010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.464"
                     /strain="DE0614"
                     /db_xref="taxon:623"
BASE COUNT          157 a           71 c           69 g          123 t
ORIGIN      
        1 taattaatac gactcactat agggagaagg gacacaaaaa gttttctctt ctaggacaac
       61 taaccgtaac ttatcgcatc ctttcagggg cgggatgacc aattacagac accaagcgtt
      121 ctacttaaag tgaatgcttc ctgtgtgctc tgggtcagtc gataagtgaa tagcaataag
      181 acacaatcat cttaattatg gtctctagtc cctacagggg cttaacttaa agttaactta
      241 aagacacaat taaagattaa cttatagtat tacttaaagt taacttaaag acacaattag
      301 agattaactt taagtattaa ataaaagatt taattaaaga ccaattaaag attaactcta
      361 agggaatacc ttaaggggtg atgatattta taataatcaa tcaccaaacc tataggtctt
//
LOCUS       DADLTY010000458          418 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.465, whole genome shotgun sequence.
ACCESSION   DADLTY010000458 DADLTY010000000
VERSION     DADLTY010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.465"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..373
                     /locus_tag="JN334_004556"
     CDS             <1..373
                     /locus_tag="JN334_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7440417.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT          121 a          100 c          105 g           92 t
ORIGIN      
        1 aacagcagca gatctgttac ttcagttatc tacggcacaa cgtcagggcc gttataaaac
       61 gacgcttcag cgtggagtaa tggccccccg cctgctcatc attgatgaaa taggctatct
      121 gccgttcagt caggaagaag caaaactgtt cttccaggtc atcgctaaac gttacgaaaa
      181 gagcgcaatg atcctgacat ccaatctacc gttcgggcag tgggatcaaa cgttcgccgg
      241 tgatgcagca ctgacctcag cgatgctggg ccgtatctta caccactcac atgtcgttca
      301 aatcaaagga gaaagctatc gactcagaca gaaacgaaag gccggggtta tagcagaagc
      361 taatcctgag taaaacggtg gatcaatatt gggccgttgg tggagatata agtggatc
//
LOCUS       DADLTY010000459          413 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.466, whole genome shotgun sequence.
ACCESSION   DADLTY010000459 DADLTY010000000
VERSION     DADLTY010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.466"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..264
                     /locus_tag="JN334_004557"
     CDS             <1..264
                     /locus_tag="JN334_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7440418.1"
                     /translation="FYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISD
                     EDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            288..>413
                     /locus_tag="JN334_004558"
     CDS             288..>413
                     /locus_tag="JN334_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7440419.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVM"
BASE COUNT          112 a           92 c          107 g          102 t
ORIGIN      
        1 ttttaccgca atgatgaatt tgtctttgac tacagactca aagcggaaga tgagcgtgat
       61 gcccttctgg ctcaggtcag tgcccggaca ggggaatggg aagaagacct gctgctgggg
      121 ttaatcagcg acgaagataa agaaaagctg aaagcctgtc gtatttacgc gaaatcgctg
      181 caggcgatgg atttcagcac catcactgat aaatcctcat acaacgccat tgaatggccc
      241 gcctctccgg aaggttcttc ctgatttaat ttatcgcgag aaaaacaatg tctgtagtga
      301 tatcaggtgc gctgactgat ggcgcaggta tccccatgtc cggataccat attattctga
      361 aatcccgggt aaacaccccg gaagtggtga tgaacactgt tgctgatgtg atg
//
LOCUS       DADLTY010000460          393 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.467, whole genome shotgun sequence.
ACCESSION   DADLTY010000460 DADLTY010000000
VERSION     DADLTY010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.467"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..275
                     /locus_tag="JN334_004559"
     CDS             <1..275
                     /locus_tag="JN334_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440420.1"
                     /translation="GQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRF
                     CAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVASI"
BASE COUNT          123 a           79 c           98 g           93 t
ORIGIN      
        1 acggacaggt aattccgtta aattacggaa acttcgatgc ccgctataaa tatcgaacag
       61 agggggtaca ggatgtacgg tatggccatg aaatgtatta cagccccggc agtaacaccg
      121 tttcgtggag attttgcgca ccttcgggac acgggctgtc agggatggcg atatcggata
      181 ccggccgtaa ctcagcggat aacgttgacg gtgtgtatta ccgaccactg caaaaactga
      241 ttaatggcac ctggtataac gtagcgagta tttaacaatg ttgcatttaa aaaatattac
      301 tgcgggtaat ccgaaaacag cagaacaata tcagatgaca aaacgatatt cggtcacctg
      361 gcttttttca gaagacggaa aaaactggta tga
//
LOCUS       DADLTY010000461          392 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.468, whole genome shotgun sequence.
ACCESSION   DADLTY010000461 DADLTY010000000
VERSION     DADLTY010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.468"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            241..>392
                     /locus_tag="JN334_004560"
     CDS             241..>392
                     /locus_tag="JN334_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440421.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIR
                     KMLEEL"
BASE COUNT          109 a           99 c           89 g           95 t
ORIGIN      
        1 taggatgccc tgtagtttcg tatagcctga tgcctagaga gaccctagag agaccctaga
       61 gtggctccta gagagaccct cccagtagct ccgcacagct acatcgtcat catcatagta
      121 ttcaatctca aagtcattct gtttgcatcc tgttgtcctg ctggtctccc ttcgcttggg
      181 gactttgagt aggtcagtgg gggtgcgact tcagggtgca aacaaaaagg aaatcaagcg
      241 atgtctcaac aaccacaaca accacaacaa gaaatcagca tcattaacct cgaccagctt
      301 gtgtccatga cctcagttga aattgctgag ctgacaggca aggagcaccg aaatgtcctt
      361 cgtgatattc gtaagatgct cgaagaactt aa
//
LOCUS       DADLTY010000462          388 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.469, whole genome shotgun sequence.
ACCESSION   DADLTY010000462 DADLTY010000000
VERSION     DADLTY010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.469"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(40..>388)
                     /locus_tag="JN334_004561"
     CDS             complement(40..>388)
                     /locus_tag="JN334_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7440422.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           92 a           89 c           87 g          120 t
ORIGIN      
        1 ttcgtgaagc tgttgaaggg tggattctta acggtggcgt taatcagaaa tgctcctgat
       61 gtgccagaac aatttatcag cgtgaagctc atacgctttg cgctgagcct ctatccagtc
      121 gtgtttattg tatacggaca ttatcccgcc aagttcatga ccgagcattc gctcagtaac
      181 atgaggctcg acattcatct cggacagccg tgtaacaagc gttcttctga aatcatgtgt
      241 tcgccagtaa cctagatcca tccctcccct tattctattt acgaatcggt tggctgcagc
      301 gatgctgatc ggtttgttta tgtcttctcc agggaaaaga acatcattgt acattgtcat
      361 ggccttttcg agaaaaggct taatttgt
//
LOCUS       DADLTY010000463          381 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.470, whole genome shotgun sequence.
ACCESSION   DADLTY010000463 DADLTY010000000
VERSION     DADLTY010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.470"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(273..>381)
                     /locus_tag="JN334_004562"
     CDS             complement(273..>381)
                     /locus_tag="JN334_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HAY7440423.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          106 a           87 c           77 g          111 t
ORIGIN      
        1 tcccgtaaag acaggagtca agttcccgct ccgacataac tgcgtgcaat ggattttgaa
       61 ggaaccgtca gcccattccc cggaggcaaa catgggctgg caaaagtgta gcggtattag
      121 gcacagctat ttaggaaaga gggaccatct ccatctcatt aaatggacca cgccagacaa
      181 ttcactgtca tgctgatgct gcactgtggg gacagaatca ctcatgatga cttcgataat
      241 cgacgacatt attatttggc cgagctttta ggtcatatct caaatcgttt gcctaatatt
      301 gctccatttt ttaataaaaa atcaatcatt tttgaatctt tgcttttcat tgcattgtca
      361 agcatagtgt ccccactatt t
//
LOCUS       DADLTY010000464          376 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.471, whole genome shotgun sequence.
ACCESSION   DADLTY010000464 DADLTY010000000
VERSION     DADLTY010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.471"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(1..279)
                     /locus_tag="JN334_004563"
     CDS             complement(1..279)
                     /locus_tag="JN334_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440424.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
BASE COUNT           85 a           90 c          113 g           88 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcggg
//
LOCUS       DADLTY010000465          372 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.472, whole genome shotgun sequence.
ACCESSION   DADLTY010000465 DADLTY010000000
VERSION     DADLTY010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.472"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..311)
                     /locus_tag="JN334_004564"
     CDS             complement(<1..311)
                     /locus_tag="JN334_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7440425.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DADLTY010000466          367 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.473, whole genome shotgun sequence.
ACCESSION   DADLTY010000466 DADLTY010000000
VERSION     DADLTY010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.473"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            67..>367
                     /locus_tag="JN334_004565"
     CDS             67..>367
                     /locus_tag="JN334_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY7440426.1"
                     /translation="MTESSDCESVLVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLH"
BASE COUNT           89 a           99 c           89 g           90 t
ORIGIN      
        1 tgtaacgtgg atgccggtac ctgctctccg tggttatctg gttaacccat atacaaggga
       61 gacagaatga ccgaatccag cgattgcgaa tccgtcctgg tctttatcgg cgttgatgtc
      121 ggtaaagata cgcatcacgc tgttgccatt aatcgttcag gtaaacgcct gttcgataaa
      181 gcattaccca acgacgaaaa caaactcagg tcgctaatat ctgacctgaa acaacatggt
      241 cagatactgc tggttgttga tcagccagct accatcggtg cgttacctgt cgccgttgcc
      301 cgctcagaag gagtccttgt cggatacctc cctggactgg ccatgcgccg catagccgac
      361 ttacacg
//
LOCUS       DADLTY010000467          363 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.474, whole genome shotgun sequence.
ACCESSION   DADLTY010000467 DADLTY010000000
VERSION     DADLTY010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.474"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..>363
                     /locus_tag="JN334_004566"
     CDS             <1..>363
                     /locus_tag="JN334_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7440427.1"
                     /translation="QIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQL
                     WHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMEL
                     TSHLLTAAAFGTMKNSEN"
BASE COUNT           76 a          123 c          102 g           62 t
ORIGIN      
        1 accagattgt gaatcgcctg gacatcatgc tgccgggcaa tcgccccttc gttgccccca
       61 gtgccgttat tcaggcccgc cagcgcctgg gaagtgaggc tgtccgccgc gtgttcacga
      121 aaacagcgca gctctggcat aacgccacgc cgcatccgca ctggtgcggc ctgaccctgc
      181 tggccatcga tggtgtgttc tggcgcacac cggatacacc agagaacgat gcagccttcc
      241 cccgccagac acatgccggg aacccggcgc tctacccgca ggtcaaaatg gtctgccaga
      301 tggaactgac cagccatctg ctgacggctg cagccttcgg cacgatgaag aacagcgaaa
      361 atg
//
LOCUS       DADLTY010000468          363 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.475, whole genome shotgun sequence.
ACCESSION   DADLTY010000468 DADLTY010000000
VERSION     DADLTY010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.475"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            51..>363
                     /locus_tag="JN334_004567"
     CDS             51..>363
                     /locus_tag="JN334_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY7440428.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LP"
BASE COUNT          104 a           88 c           78 g           93 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctg
//
LOCUS       DADLTY010000469          359 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.476, whole genome shotgun sequence.
ACCESSION   DADLTY010000469 DADLTY010000000
VERSION     DADLTY010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.476"
                     /strain="DE0614"
                     /db_xref="taxon:623"
BASE COUNT           76 a          102 c           90 g           91 t
ORIGIN      
        1 agcctgcgct gatgcagcat gtccctgaag gcgtgattca ggcaccagaa tcacccgacg
       61 ccccggaggc gctgccgggg tgtcgttcca tttctgatga tgacctgtgc gcctggtgta
      121 cccgtctgct ttaccgtccc ggagaaatca gtctgtgcag actgagtata caggcaatgg
      181 catctggcca tccgtctgtg accggaacgg ttacgcgtac ggctgtccgg aatttcagcc
      241 gaatatcatt catccctgat attaccgggt catcacggtc agtgatgaac gtgatgttgc
      301 cagctcccga cctttactcc ctctcattta ttcacattac aaggatttca tacatgaaa
//
LOCUS       DADLTY010000470          349 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.478, whole genome shotgun sequence.
ACCESSION   DADLTY010000470 DADLTY010000000
VERSION     DADLTY010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.478"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..>349
                     /locus_tag="JN334_004568"
     CDS             <1..>349
                     /locus_tag="JN334_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7440429.1"
                     /translation="RWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKT
                     RTARLWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRAL
                     YESGRITEAACMAH"
BASE COUNT           70 a           96 c          109 g           74 t
ORIGIN      
        1 cgctggaccg gtgccgttgc tgaactgctg gagccgctgt atgacgtcct gcgccagtat
       61 gtactgatgc ccggtaaagt tcatgcggat gatatccccg ttccggtcca ggagccgggc
      121 agtggtaaaa caagaactgc ccggctgtgg gtctatgtcc gtgatgaccg taacgccggt
      181 tcacagatgc ccccggcggt ctggttcgcg tacagcccgg accggaaagg tatccatcca
      241 cagaatcacc tagccggtta cagcggtgtg cttcaggcag atgcttacgg tggttaccgg
      301 gcgttatacg aatccggcag aataacggaa gctgcgtgta tggcccatg
//
LOCUS       DADLTY010000471          340 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.479, whole genome shotgun sequence.
ACCESSION   DADLTY010000471 DADLTY010000000
VERSION     DADLTY010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.479"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..171
                     /gene="xisR"
                     /locus_tag="JN334_004569"
     CDS             <1..171
                     /gene="xisR"
                     /locus_tag="JN334_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HAY7440430.1"
                     /translation="LMLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWY
                     NYPKINQFIKDS"
     gene            173..>340
                     /locus_tag="JN334_004570"
     CDS             173..>340
                     /locus_tag="JN334_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HAY7440431.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHQISS"
BASE COUNT          108 a           64 c           82 g           86 t
ORIGIN      
        1 ctgatgcttc gcactggtct gggtgccaga caaattgaag cataccgcca gaactgttgg
       61 gtggaaggct tccacttcaa acgagtatct cctttaggga agccagacag taagcgaggg
      121 attatctggt acaactatcc gaagataaat cagtttatca aagactcatg atatgtctaa
      181 attaccaaca ggtgtcgaga ttcgaggtaa atacattcgc atctggttca tgtttcgagg
      241 aaaacgatgt cgggaaacat tgaaaggctg ggagattact aacagtaaca ttaaaaaggc
      301 cgggaattta agagcgttga tagttcatca aatcagttcc
//
LOCUS       DADLTY010000472          333 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.480, whole genome shotgun sequence.
ACCESSION   DADLTY010000472 DADLTY010000000
VERSION     DADLTY010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.480"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(45..332)
                     /locus_tag="JN334_004571"
     CDS             complement(45..332)
                     /locus_tag="JN334_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7440432.1"
                     /translation="MDEEYRKDLQLWFGLTHASFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT           92 a           85 c           66 g           90 t
ORIGIN      
        1 tataagccat tatcaaaccc acccgtagat aggctttgta atggctactt cttcagaaat
       61 gattcgatga attcacgtcg aggatgacga tagttcagaa tatcttctgg catcctcata
      121 aagcggttgt tgccgtcttt ggcagtaaca aaacagctgt gaactccgca gacatccgtt
      181 ttgatcgtgt cgctatactc aaaaagcaac tgagccatct tctcttgcca ttctggaggc
      241 atagcctcca tgaatactcg cggcatcacg cagaacgacg catgcgtaag accaaaccac
      301 agttgcaggt ctttacgata ttcttcatcc atc
//
LOCUS       DADLTY010000473          332 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.481, whole genome shotgun sequence.
ACCESSION   DADLTY010000473 DADLTY010000000
VERSION     DADLTY010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.481"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            14..>332
                     /locus_tag="JN334_004572"
     CDS             14..>332
                     /locus_tag="JN334_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7440433.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          107 a           78 c           84 g           63 t
ORIGIN      
        1 ataaaacaag ggaatgaaca atgaactccc cgatgatatt gagctgctta aagccatgtt
       61 gcgtaagcaa cagagtcggc ttcgacagta tgcctgtcag gtcgcgggct atgagcagga
      121 aattgaacgg ctgaaagcgc aactcgacag gttgcgtcgt atgttgttcg gccagagttc
      181 agagaaaaag cgtcataagc ttgaaaatca gatccgacag gcagaaaaac gactgtcgga
      241 actggaaaac cggctgaaca cagccagaaa tcttctggaa gatgcatcgt cagtcacaga
      301 ttcacctgac accagtcccc cgtcagaaaa cc
//
LOCUS       DADLTY010000474          332 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.482, whole genome shotgun sequence.
ACCESSION   DADLTY010000474 DADLTY010000000
VERSION     DADLTY010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.482"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            51..>332
                     /locus_tag="JN334_004573"
     CDS             51..>332
                     /locus_tag="JN334_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAY7440434.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT           83 a           70 c           98 g           81 t
ORIGIN      
        1 cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt atggcgaaac
       61 cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc gtgcatcagg
      121 acggtcgggt cacgattcgg cagttgtccg atgaaacagg tatcagtcgt gcgtctgtcg
      181 gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc tacggcttat
      241 tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc tcgagagtaa
      301 aggtgaggaa accggttgtt gttgatccgg ac
//
LOCUS       DADLTY010000475          329 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.483, whole genome shotgun sequence.
ACCESSION   DADLTY010000475 DADLTY010000000
VERSION     DADLTY010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.483"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..>329
                     /locus_tag="JN334_004574"
     CDS             <1..>329
                     /locus_tag="JN334_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7440435.1"
                     /translation="KVIAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIA
                     LEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTE
                     TSNIQLA"
BASE COUNT           90 a           79 c           76 g           84 t
ORIGIN      
        1 gtaaagttat tgctccagct tgtaccctgg tagctgcgac gaaagattca gtagtcacat
       61 tgccaaatgt cagcgccacc aagttgcaaa ctaatggagc agtctctggc gttaaaactg
      121 atgtaccaat tgccttagaa ggctgtgatg ttactgtaac taaaaatgcg acgtttacct
      181 ttagtggtac ggctgacggc gtgcaaccta cagcatttgc caaccaggct accaccgatg
      241 cagcgacgaa tgttgctcta caaatgtatc tgccagatgg ttctacatcg gtaactcccg
      301 gtaccgaaac gtcgaatatt cagttagcg
//
LOCUS       DADLTY010000476          329 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.484, whole genome shotgun sequence.
ACCESSION   DADLTY010000476 DADLTY010000000
VERSION     DADLTY010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.484"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            1..329
                     /locus_tag="JN334_004575"
                     /pseudo
     CDS             1..329
                     /locus_tag="JN334_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           82 a           88 c           84 g           75 t
ORIGIN      
        1 ttccactaat aaagaacagt cagatgctca ggccgagatc cgccgtctcc agaaagagct
       61 gaaacgggtt accgacgaac gggacatatt aaaaaaagcc gcggcgtact tcgcaaagct
      121 gtccgactga ggtacgcctt tatccgtgac aactcctgtt gctggcctgt tcgcctgctc
      181 tgtcgggtgc tggatgttca tcccagtggt ttttacgcct ggcttcagca gccgcattca
      241 caacgccatc aggcagacct gagactgaca ggacagatta aacagttctg gctggaatcg
      301 ggatgcgtct atggttatcg caaaatcca
//
LOCUS       DADLTY010000477          326 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.485, whole genome shotgun sequence.
ACCESSION   DADLTY010000477 DADLTY010000000
VERSION     DADLTY010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.485"
                     /strain="DE0614"
                     /db_xref="taxon:623"
BASE COUNT          111 a           56 c           61 g           98 t
ORIGIN      
        1 aatttacgta gctcacgctt aacagagaaa atacttatat atcaaaggct tggttaaaaa
       61 tgattaaaat gcgctaagtc tttgaaatca aaagagaatt taatgattta ctctaatcag
      121 cttcgttaca ctggcaaaga tggtgtttac ttccatctac aaggccaacc agataatttc
      181 ttgtcactat cagcaactga aaacatggaa tttgatagcc ctatgatggt taccactcaa
      241 aatatgcaat cagggcagac tgtaacagat aatgtaatgc gttcacctag aactatttca
      301 atcagtggtg tggttgtggt tggtga
//
LOCUS       DADLTY010000478          305 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.487, whole genome shotgun sequence.
ACCESSION   DADLTY010000478 DADLTY010000000
VERSION     DADLTY010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.487"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..>305)
                     /locus_tag="JN334_004576"
     rRNA            complement(<1..>305)
                     /locus_tag="JN334_004576"
                     /product="16S ribosomal RNA"
BASE COUNT           56 a          104 c           70 g           75 t
ORIGIN      
        1 gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc ttcgccaccg
       61 gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc cccctctacg
      121 agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga tttcacatct
      181 gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac gcttgcaccc
      241 tccgtattac cgcggctgct ggcacggagt tagccggtgc ttcttctgcg ggtaacgtca
      301 atgag
//
LOCUS       DADLTY010000479          304 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.488, whole genome shotgun sequence.
ACCESSION   DADLTY010000479 DADLTY010000000
VERSION     DADLTY010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.488"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..>304
                     /locus_tag="JN334_004577"
     CDS             <1..>304
                     /locus_tag="JN334_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7440436.1"
                     /translation="IEAEVRGCSAEQRLAARKARAAPLMQSLYDRIQQQMKTLSRHSD
                     TAKAFAYLLKQWKALNVYCSNGWVEIDNNIAENALRAVSLGRKNFLFFGSDHGGERG"
BASE COUNT           82 a           75 c           91 g           56 t
ORIGIN      
        1 ccatcgaggc agaagtccgg ggatgttcag cagaacagcg tctggcggca agaaaagcaa
       61 gagccgcgcc actgatgcag tcactgtatg accggataca gcaacagatg aaaacactgt
      121 cgcgtcactc ggatacggca aaagcgttcg catacctact gaaacagtgg aaggccctga
      181 acgtgtactg cagtaatggc tgggtggaaa tcgacaacaa catcgctgaa aatgccctgc
      241 gggcggtcag tctgggtcgt aaaaacttcc tgttcttcgg ctctgaccat ggtggtgagc
      301 gggg
//
LOCUS       DADLTY010000480          301 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.489, whole genome shotgun sequence.
ACCESSION   DADLTY010000480 DADLTY010000000
VERSION     DADLTY010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.489"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            61..>301
                     /locus_tag="JN334_004578"
     CDS             61..>301
                     /locus_tag="JN334_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAY7440437.1"
                     /translation="MAELAIELDDMTIITEKLRDMKNALSKLNHDIEEDTKRIKAITT
                     GSLKDIDRTTKEGMVETQLLIKGVLKEIVIDAAKRR"
BASE COUNT          117 a           55 c           64 g           65 t
ORIGIN      
        1 actgacacta ttgatagcct taaattacag cgtgaagatt tgcagcgtaa aatctcaaaa
       61 atggcagaac ttgctattga actcgatgat atgacaatta tcacagagaa attacgcgac
      121 atgaagaatg cgctttcaaa actcaatcat gatatcgaag aggatacaaa gcggataaag
      181 gccatcacaa ctggaagcct caaagatatt gacaggacaa caaaggaagg gatggtagag
      241 acacagctac ttatcaaggg cgtactcaaa gaaatagtta tagatgccgc taaacgtcga
      301 t
//
LOCUS       DADLTY010000481          299 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.490, whole genome shotgun sequence.
ACCESSION   DADLTY010000481 DADLTY010000000
VERSION     DADLTY010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.490"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..>299
                     /locus_tag="JN334_004579"
     CDS             <1..>299
                     /locus_tag="JN334_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY7440438.1"
                     /translation="DVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALE
                     MAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERF"
BASE COUNT           69 a           75 c           95 g           60 t
ORIGIN      
        1 gatgtgacct atatctggac gggtaagcgc tgggcgtacc tcgccgttgt tctcgacctg
       61 ttcgcaagaa aaccagtggg ctgggccatg tcgttctcgc cggacagcag gctcaccatg
      121 aaagcgctgg aaatggcatg ggaaacccgt ggtaagcccg gcggggtgat gttccacagc
      181 gatcagggca gtcattatac gagcaggcag ttccggcagt tattgtggcg ataccagatc
      241 agacagagta tgagccggcg cggaaactgc tgggataaca gcccaatgga acgcttctt
//
LOCUS       DADLTY010000482          298 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.491, whole genome shotgun sequence.
ACCESSION   DADLTY010000482 DADLTY010000000
VERSION     DADLTY010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.491"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            1..298
                     /locus_tag="JN334_004580"
                     /pseudo
     CDS             1..298
                     /locus_tag="JN334_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           63 a           72 c           93 g           70 t
ORIGIN      
        1 gttgaatatg gacgggcaaa aaagtggata gcgcacgcgc ccttattgcc cggggatggg
       61 gagtaagctt agtcagccgt tgtctccggg tgtcgcgtgc gcagttgcac gtcattctca
      121 gacgaaccga tgactggatg gatggccgcc gcagtcgtca cactgatgat acggatgtgc
      181 ttctccgtat acaccatgtt atcggagagc tgcccacgta tggttatcgt cgggtatggg
      241 cgctgcttcg cagacaggca gaacttgatg gtatgcctgc gatcaatgcc aaacgtgt
//
LOCUS       DADLTY010000483          296 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.492, whole genome shotgun sequence.
ACCESSION   DADLTY010000483 DADLTY010000000
VERSION     DADLTY010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.492"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..>296
                     /locus_tag="JN334_004581"
     CDS             <1..>296
                     /locus_tag="JN334_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7440439.1"
                     /translation="YGYRRVWALLRRQAELDGMPAINAKRIYRLMRQNALLLERKLAV
                     PPSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           68 a           73 c           90 g           65 t
ORIGIN      
        1 tatggttatc gtcgggtatg ggcgctgctt cgcagacagg ccgaacttga tggtatgcct
       61 gcgatcaatg ccaaacgtat ttaccggctc atgcgccaga atgcgctgtt gcttgagcga
      121 aaactcgctg taccgccatc gaaacgggca cataccggca gagtggctgt gaaagaaagt
      181 aatcagcgat ggtgctctga cgggttcgag ttccgctgtg ataacggaga aaaactgcga
      241 gtcacgttcg cgctggactg ctgtgaccgt aaggcactgc actgggcggt cactac
//
LOCUS       DADLTY010000484          291 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.493, whole genome shotgun sequence.
ACCESSION   DADLTY010000484 DADLTY010000000
VERSION     DADLTY010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.493"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..>291
                     /locus_tag="JN334_004582"
     rRNA            <1..>291
                     /locus_tag="JN334_004582"
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           71 c           94 g           54 t
ORIGIN      
        1 ctggaaacgg tagctaatac cgcataacgt cgcaagacca aagaggggga ccttcgggcc
       61 tcttgccatc ggatgtgccc agatgggatt agctagtagg tggggtaacg gctcacctag
      121 gcgacgatcc ctagctggtc tgagaggatg accagccaca ctggaactga gacacggtcc
      181 agactcctac gggaggcagc agtggggaat attgcacaat gggcgcaagc ctgatgcagc
      241 catgccgcgt gtatgaagaa ggccttcggg ttgtaaagta ctttcagcgg g
//
LOCUS       DADLTY010000485          289 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.494, whole genome shotgun sequence.
ACCESSION   DADLTY010000485 DADLTY010000000
VERSION     DADLTY010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.494"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..>289
                     /locus_tag="JN334_004583"
     CDS             <1..>289
                     /locus_tag="JN334_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY7440440.1"
                     /translation="TLMDKGYYSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKLGKG
                     DHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGG"
BASE COUNT           84 a           68 c           89 g           48 t
ORIGIN      
        1 gacgttaatg gataaaggtt attactcact gggactgtta aatggctgga gcctggcggg
       61 agaacaccgc cactggatga tacctctcag aaagggagcg caatatgaag agctcagaaa
      121 actgggtaaa ggcgatcatc tggtgaagct gaaaaccagc ccgcaggcac gaaaaaagtg
      181 gccgggactg ggaaatgaag tgacagcccg cctgctgacc gtgacgcgca aaggaaaagt
      241 ctgccatctg ctgacgtcga tgacggacgc catgcgcttc cccggagga
//
LOCUS       DADLTY010000486          289 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.495, whole genome shotgun sequence.
ACCESSION   DADLTY010000486 DADLTY010000000
VERSION     DADLTY010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.495"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            complement(<1..>289)
                     /gene="intI1"
                     /locus_tag="JN334_004584"
     CDS             complement(<1..>289)
                     /gene="intI1"
                     /locus_tag="JN334_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY7440441.1"
                     /translation="RERIRYLHYSLRTEQAYVHWVRAFIRFHGVRHPATLGSSEVEAF
                     LSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQEIGRPRPSRRLPVV"
BASE COUNT           69 a           89 c           85 g           46 t
ORIGIN      
        1 gcaccaccgg caagcgccgc gacggccgag gtcttccgat ctcctgaagc cagggcagat
       61 ccgtgcacag caccttgccg tagaagaaca gcaaggccgc caatgcctga cgatgcgtgg
      121 agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc
      181 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
      241 aagcctgttc ggttcgtaaa ctgtaatgca agtagcgtat gcgctcacg
//
LOCUS       DADLTY010000487          288 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.496, whole genome shotgun sequence.
ACCESSION   DADLTY010000487 DADLTY010000000
VERSION     DADLTY010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.496"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..269
                     /locus_tag="JN334_004585"
     CDS             <1..269
                     /locus_tag="JN334_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7440442.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
BASE COUNT           73 a           78 c           82 g           55 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtac
//
LOCUS       DADLTY010000488          277 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.497, whole genome shotgun sequence.
ACCESSION   DADLTY010000488 DADLTY010000000
VERSION     DADLTY010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.497"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            6..277
                     /locus_tag="JN334_004586"
                     /pseudo
     CDS             6..277
                     /locus_tag="JN334_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT           79 a           64 c           81 g           53 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgtccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtgga
//
LOCUS       DADLTY010000489          274 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.denovo.498, whole genome shotgun sequence.
ACCESSION   DADLTY010000489 DADLTY010000000
VERSION     DADLTY010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.denovo.498"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            1..224
                     /locus_tag="JN334_004587"
                     /pseudo
     CDS             1..224
                     /locus_tag="JN334_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT           70 a           66 c           85 g           53 t
ORIGIN      
        1 tcgttctcgc cggacagcag gcttaccatg aaagcactgg aaatggcatg ggaaacccgt
       61 ggtaagcccg tcggggtgat gttccaagcg atcaaggcag tcattatacg agcaggcagt
      121 tccggcagtt actgtggcga taccagatca gacagagtat gagccggcgt ggaaactgct
      181 gggataacag cccaatggag cgcttcttca ggagtctgaa gaactaatgg gtgccagcga
      241 cgggctatgt aagcttcagc gatgcagctc acgc
//
LOCUS       DADLTY010000490        10098 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.guided.150, whole genome shotgun sequence.
ACCESSION   DADLTY010000490 DADLTY010000000
VERSION     DADLTY010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.guided.150"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..246
                     /locus_tag="JN334_004588"
     CDS             <1..246
                     /locus_tag="JN334_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021138877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7440443.1"
                     /translation="QRYRASGLNLVTAAVVLWNTVYLERAAHALRGNGHAVDDALLQY
                     LSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
     gene            325..1329
                     /locus_tag="JN334_004589"
     CDS             325..1329
                     /locus_tag="JN334_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HAY7440444.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLAT
                     CPATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISR
                     LSTLLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCV
                     GTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLL
                     VQCARVFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA
                     "
     gene            complement(1511..1687)
                     /locus_tag="JN334_004590"
     CDS             complement(1511..1687)
                     /locus_tag="JN334_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HAY7440445.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            complement(1916..1993)
                     /locus_tag="JN334_004591"
                     /pseudo
     CDS             complement(1916..1993)
                     /locus_tag="JN334_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            2017..2832
                     /gene="sul2"
                     /locus_tag="JN334_004592"
     CDS             2017..2832
                     /gene="sul2"
                     /locus_tag="JN334_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAY7440446.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2893..3696
                     /locus_tag="JN334_004593"
                     /note="aph(3'')-Ib"
     CDS             2893..3696
                     /locus_tag="JN334_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAY7440447.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            3696..4532
                     /locus_tag="JN334_004594"
     CDS             3696..4532
                     /locus_tag="JN334_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HAY7440448.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(4504..5043)
                     /locus_tag="JN334_004595"
     CDS             complement(4504..5043)
                     /locus_tag="JN334_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7440449.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFGVVCGKGRNPTLRLWPAIFSGEIDV
                     FPGDRRSRLISSMTSVAPA"
     gene            complement(5253..6113)
                     /locus_tag="JN334_004596"
     CDS             complement(5253..6113)
                     /locus_tag="JN334_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HAY7440450.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(6296..6853)
                     /locus_tag="JN334_004597"
     CDS             complement(6296..6853)
                     /locus_tag="JN334_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7440451.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            7017..10025
                     /locus_tag="JN334_004598"
     CDS             7017..10025
                     /locus_tag="JN334_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAY7440452.1"
                     /translation="MPVDFLTTEQELNYGRYVAEPNDVQLARYFHLDERDLAFINQRR
                     GRHNRLGIALQLTTARFLGTFLTDLTRVLPGVQQFVAVQLNIRRPEVLTRYAERVTTL
                     REHTALIKEYYGYHEFGDFPWSFRLKRLLYTRAWLSNERPGLMFDFATAWLLQNKVLL
                     PGATTLVRLVSEIRERANQRLWKKLAALPDSWQTARVTELLDIPEGQRTSPLEQLKKG
                     PVTVSGPAFTEALERYIRLRNLEFSRLNFTGLPAIQLRNLARYAGMASVKYIARMPQQ
                     RKLAVLTAFVKAQETAALDEAVDVLDMLILDITRAAKKTGQKKRLRTLKDLDRAALIL
                     ARACSLLLDEQADDAELRETIFNSIPKSRLAESVSKVNELARPQNNNFHDEMVEQYGR
                     VKRFLPAVLRDLHFQAAPAGEHTLSAIHYLTELNGSKKRILDNAPEHIITGPWKRLVY
                     DAEGRIQRAGYSLCLLERLQDALRRRDIWLENSDRWGNPREKLLQGEEWQAQRVPVCR
                     ALGHPTDGHKGVQQLAVQLDETWKTVASRFEGNAEVHICHDGKHPSLTISSLEKLEEP
                     TSLHRLNSRVRLLLPPVDLTELLLEIDARTGFTREFTHVSESGARAQDLHISLCAVLM
                     AEACNIGLEPLIKHNIPALTRHRLSWVKQNYLRAETLVSANARLVDFQSTLELAGRWG
                     GGEVASADGMRFVTPVKTVNSGPNRKYFGSGRGITWYNFVSDQYSGFHGIVVPGTLRD
                     SIFVLEGLLEQQTGLNPVEIMTDTAGTSDIIFGLFWLLGYQFSPRLADAGEAVFWRVD
                     KSANYGALDELARGCADLSKAEDQWDEMMRTAGSLKLGTIHASELIRSLLKSSRPSGL
                     AQAIMEVGRVNKTLYLLNYIDDEDYRRRILTQLNRGEGRHAVARAICYGQRGEIRKRY
                     REGQEDQLGALGLVTNAVVLWNTLYMQEALSWIRSNGEEIGVEDIARLSPLMHGHINM
                     LGHYTFTLPEDILKGELRELNFNLNNELTS"
BASE COUNT         2355 a         2637 c         2684 g         2422 t
ORIGIN      
        1 cagcgctacc gggctagcgg cctcaatctg gtaacggctg ccgtcgtgtt gtggaacacg
       61 gtctatctgg aacgggctgc gcacgcgctg cgtggcaacg gccatgccgt tgatgacgcg
      121 ctgttgcagt acctgtcgcc gctcggttgg gagcacatca acctcaccgg cgattacctc
      181 tggcgcagca gcgccaagat cggcgcgggc aagttcaggc cgctacgacc gctgcaaccg
      241 gcttagcgtg ctttatttaa tgagatggtc actccctcct tcccagtact atgctgagga
      301 caggctttca ttcggagaac catcatggaa aacattgcgc ttattggtat cgatctgggt
      361 aagaactctt tccatattca ttgtcaggat catcgtggga aggccgttta ccgtaaaaaa
      421 ttcacccgac caaagctaat cgaatttctg gcgacatgcc cggcaacaac catcgcgatg
      481 gaagcctgtg gcggttctca ctttatggca cgcaagctgg aagagttagg gcattttcca
      541 aagctgatat caccgcaatt tgtccgccca ttcgttaaaa gcaacaaaaa tgacttcgtt
      601 gatgctgaag ctatctgtga agcagcatca cgtccatcta tgcgtttcgt gcagcccaga
      661 accgaatctc agcaggcaat gcgagctctg catcgtgtcc gtgaatccct ggttcaggat
      721 aaggtgaaaa caactaatca gatgcatgct tttctgctgg aatttggtat cagcgttccg
      781 cgaggtgctg ccgttattag tcgactgagt acccttcttg aggacagtag tttgcctctt
      841 tatctcagcc agttactgct gaaattacaa cagcattatc actatcttgt tgagcagatt
      901 aaagatctgg aatctcagtt gaaacgaaag ttggacgaag atgaggttgg acagcgcttg
      961 ctgagtattc cctgcgttgg aacgctgact gccagtacta tttcaactga gattggcgac
     1021 gggaagcagt acgccagcag ccgtgacttt gcggcggcaa cagggctggt accccgacag
     1081 tacagcacgg gaggtcggac gacattgtta gggattagca agcggggcaa caaaaagatc
     1141 cgaactttgt tggttcagtg tgccagggta ttcatacaaa aactggaaca ccagtctggc
     1201 aagttggccg actgggtcag ggagttgttg tgtcggaaaa gcaactttgt cgtcacctgt
     1261 gctctggcaa acaagctggc cagaatagcc tgggcactga cggcgcgaca gcaaacttac
     1321 gaagcataaa ggcagaaata caccagttta aacaatcatt catctggttt tgcgaatact
     1381 gatattgatg atactaacgg cccaccggcc tgttgaggaa cctgtaaaac ggaaaggctc
     1441 attgaagccg tatattttct ggaggttcat caggcgcgga actcatcgag gcgcgggaat
     1501 aaaatcccat tcagacgccg gatagattca agcaagccaa cttgtcgtca aaatcggtgt
     1561 tgcaaaaacg ggagtgacca tagattccgt tttctgaggc gacccccaca tagagacctt
     1621 cccggtcatt cactttgtag agtttgtccc tgggcttgag attgcgcagc ttggtatcgg
     1681 tcagcatgta ccttgtcctt cttcgtctcc gatacccact ctattgtaaa caagacattt
     1741 ttatctttta tattcaatgg cttattttcc tgctaattgg taataccatg aaaaatacca
     1801 tgctcagaaa aggcttaaca atattttgaa aaattgccta ctgagcgctg ccgcacagct
     1861 ccataggccg ctttcctggc tttgcttcca gatgtatgct attctgctcc tgcagctaat
     1921 ggagcaccgc aaacaggtta ctcgcctggg gattcccttt cgacccgagc atccgtatga
     1981 gactcatgct cgattattat tattatagaa gcccccatga ataaatcgct catcattttc
     2041 ggcatcgtca acataacctc ggacagtttc tccgatggag gccggtatct ggcgccagac
     2101 gcagccattg cgcaggcgcg taagctgatg gccgaggggg cagatgtgat cgacctcggt
     2161 ccggcatcca gcaatcccga cgccgcgcct gtttcgtccg acacagaaat cgcgcgtatc
     2221 gcgccggtgc tggacgcgct caaggcagat ggcattcccg tctcgctcga cagttatcaa
     2281 cccgcgacgc aagcctatgc cttgtcgcgt ggtgtggcct atctcaatga tattcgcggt
     2341 tttccagacg ctgcgttcta tccgcaattg gcgaaatcat ctgccaaact cgtcgttatg
     2401 cattcggtgc aagacgggca ggcagatcgg cgcgaggcac ccgctggcga catcatggat
     2461 cacattgcgg cgttctttga cgcgcgcatc gcggcgctga cgggtgccgg tatcaaacgc
     2521 aaccgccttg tccttgatcc cggcatgggg ttttttctgg gggctgctcc cgaaacctcg
     2581 ctctcggtgc tggcgcggtt cgatgaattg cggctgcgct tcgatttgcc ggtgcttctg
     2641 tctgtttcgc gcaaatcctt tctgcgcgcg ctcacaggcc gtggtccggg ggatgtcggg
     2701 gccgcgacac tcgctgcaga gcttgccgcc gccgcaggtg gagctgactt catccgcaca
     2761 cacgagccgc gccccttgcg cgacgggctg gcggtattgg cggcgctgaa agaaaccgca
     2821 agaattcgtt aactgcacat tcgggatatt tctctatatt cgcgcttcat cagaaaactg
     2881 aaggaacctc cattgaatcg aactaatatt ttttttggtg aatcgcattc tgactggttg
     2941 cctgtcagag gcggagaatc tggtgatttt gtttttcgac gtggtgacgg gcatgccttc
     3001 gcgaaaatcg cacctgcttc ccgccgcggt gagctcgctg gagagcgtga ccgcctcatt
     3061 tggctcaaag gtcgaggtgt ggcttgcccc gaggtcatca actggcagga ggaacaggag
     3121 ggtgcatgct tggtgataac ggcaattccg ggagtaccgg cggctgatct gtctggagcg
     3181 gatttgctca aagcgtggcc gtcaatgggg cagcaacttg gcgctgttca cagcctatcg
     3241 gttgatcaat gtccgtttga gcgcaggctg tcgcgaatgt tcggacgcgc cgttgatgtg
     3301 gtgtcccgca atgccgtcaa tcccgacttc ttaccggacg aggacaagag tacgccgcag
     3361 ctcgatcttt tggctcgtgt cgaacgagag ctaccggtgc ggctcgacca agagcgcacc
     3421 gatatggttg tttgccatgg tgatccctgc atgccgaact tcatggtgga ccctaaaact
     3481 cttcaatgca cgggtctgat cgaccttggg cggctcggaa cagcagatcg ctatgccgat
     3541 ttggcactca tgattgctaa cgccgaagag aactgggcag cgccagatga agcagagcgc
     3601 gccttcgctg tcctattcaa tgtattgggg atcgaagccc ccgaccgcga acgccttgcc
     3661 ttctatctgc gattggaccc tctgacttgg ggttgatgtt catgccgcct gtttttcctg
     3721 ctcattggca cgtttcgcaa cctgttctca ttgcggacac cttttccagc ctcgtttgga
     3781 aagtttcatt gccagacggg actcctgcaa tcgtcaaggg attgaaacct atagaagaca
     3841 ttgctgatga actgcgcggg gccgactatc tggtatggcg caatgggagg ggagcagtcc
     3901 ggttgctcgg tcgtgagaac aatctgatgt tgctcgaata tgccggggag cgaatgctct
     3961 ctcacatcgt tgccgagcac ggcgactacc aggcgaccga aattgcagcg gaactaatgg
     4021 cgaagctgta tgccgcatct gaggaacccc tgccttctgc ccttctcccg atccgggatc
     4081 gctttgcagc tttgtttcag cgggcgcgcg atgatcaaaa cgcaggttgt caaactgact
     4141 acgtccacgc ggcgattata gccgatcaaa tgatgagcaa tgcctcggaa ctgcgtgggc
     4201 tacatggcga tctgcatcat gaaaacatca tgttctccag tcgcggctgg ctggtgatag
     4261 atcccgtcgg tctggtcggt gaagtgggct ttggcgccgc caatatgttc tacgatccgg
     4321 ctgacagaga cgacctttgt ctcgatccta gacgcattgc acagatggcg gacgcattct
     4381 ctcgtgcgct ggacgtcgat ccgcgtcgcc tgctcgacca ggcgtacgct tatgggtgcc
     4441 tttccgcagc ttggaacgcg gatggagaag aggagcaacg cgatctagct atcgcggccg
     4501 cgatcaagca ggtgcgacag acgtcatact agatatcaag cgacttctcc tatcccctgg
     4561 gaacacatca atctcaccgg agaatatcgc tggccaaagc cttagcgtag gattccgccc
     4621 cttcccgcaa acgaccccaa acaggaaacg cagctgaaac gggaagctca acacccactg
     4681 acgcatgggt tgttcaggca gtacttcatc aaccagcaag gcggcacttt cggccatccg
     4741 ccgcgcccca cagctcgggc agaaaccgcg acgcttacag ctgaaagcga ccaggtgctc
     4801 ggcgtggcaa gactcgcagc gaacccgtag aaagccatgc tccagccgcc cgcattggag
     4861 aaattcttca aattcccgtt gcacatagcc cggcaattcc tttccctgct ctgccataag
     4921 cgcagcgaat gccgggtaat actcgtcaac gatctgatag agaagggttt gctcgggtcg
     4981 gtggctctgg taacgaccag tatcccgatc ccggctggcc gtcctggccg ccacatgagg
     5041 catgttccgc gtccttgcaa tactgtgttt acatacagtc tatcgcttag cggaaagttc
     5101 ttttaggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     5161 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     5221 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5281 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5341 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5401 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5461 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5521 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
     5581 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5641 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5701 gtcagaagta agttggcagc agtgttatca ctcatggtta tggcagcact gcataattct
     5761 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     5821 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
     5881 accgcaccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     5941 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6001 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     6061 caaaatgccg caaaaaaggg aataagggcg acacgaaaat gttgaatact catactcttc
     6121 ctttttcaat attattgaag catttaccag ggttattgtc tcatgagcgg atacatattt
     6181 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     6241 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     6301 aggccctttc gtcttcaaga attttataaa ccgtggagcg ggcaatactg agctgatgag
     6361 caatttccgt tgcaccagtg cccttctgat gaagcgtcag cacgacgttc ctgtccacgg
     6421 tacgcctgcg gccaaatttg attcctttca gctttgcttc ctgtcggccc tcattcgtgc
     6481 gttctaggat cctccggcgt tcagcctgtg ccacagccga caggatggtg accaccattt
     6541 gccccatatc accgtcggta ctgatcccgt catcaatgaa ccggactgcc acgccctgag
     6601 cgtcaaattc ctttatcagt tggatcatat cggcagtgtc gcggccaaga cggtcgagct
     6661 tcttaaccag aatgacatca ccttcctcca ccttcatcct cagcaaatcc agcccttccc
     6721 ggtctgttga actgccggat gccttatcgg taaatatacg gtttgctttc acacctgcgt
     6781 ctttgagtgc tctgacctga agatcaagag actgctgact ggttgagacc cgagcgtaac
     6841 caaaaagtcg cataaaaatg taccttaaat cgaatatcgg acaactcatg tctattatta
     6901 caaatttacg atttaataga catattaatg taacagtttt acgatgtccg ataatttata
     6961 atatttcgga cggttgcgaa attgttaatt catgaccatc aggcagggag gccggtatgc
     7021 ccgtcgattt tctgaccact gagcaggaac tgaattatgg tcgctatgtt gcagaaccca
     7081 atgacgtgca gctggcgcgc tattttcatc ttgatgagcg ggatcttgcc ttcattaacc
     7141 agcggcgggg aaggcataac cggctgggaa ttgcgcttca gctcaccacc gcccgttttc
     7201 tgggcacctt tctgacagac ttaacccggg ttctgccagg tgttcagcaa tttgtcgcgg
     7261 tgcagcttaa tattcgccgt ccggaagtcc ttacccgcta cgcagaacgg gttaccaccc
     7321 tcagagagca tactgcgctg attaaggaat attacggtta ccatgaattt ggtgatttcc
     7381 cctggtcttt ccgcctgaag cgcctgctgt acacccgcgc atggctcagt aatgagcgac
     7441 ccggccttat gtttgatttt gccaccgcgt ggctgcttca gaataaggtc ctgctgcccg
     7501 gagcaaccac acttgtacgt ctcgtcagtg aaatacgcga acgggcaaat cagcggctgt
     7561 ggaagaaact ggccgcgttg ccggacagct ggcagaccgc ccgcgtgacg gagcttctgg
     7621 acattcctga ggggcagcgt acttcaccgc tggagcaact gaaaaaggga ccggtcaccg
     7681 tcagcggccc ggcatttacc gaagcgctgg agcggtatat ccggctgcga aacctggaat
     7741 tttcccgact gaattttacc ggtctgcctg ctatacaact gcgtaatttg gcccgttacg
     7801 caggcatggc gtcggtaaaa tatatcgcgc gaatgccaca gcagagaaaa ctggccgtac
     7861 tcacagcatt cgttaaagcg caggagactg cggcgctgga tgaggccgtt gatgtgctcg
     7921 atatgctgat actggatatc actcgtgcgg cgaagaaaac ggggcagaaa aaacggctca
     7981 ggacgcttaa agatcttgat cgtgctgcgc taatattggc gcgggcatgt tcattgttgc
     8041 tggatgaaca ggctgacgat gctgaactga gggagaccat attcaacagc ataccgaaaa
     8101 gcaggctggc agaatccgtc agcaaggtaa atgagctggc ccggcctcag aacaacaatt
     8161 tccatgacga aatggtggag cagtacgggc gggtaaagcg ctttcttccg gcggtattgc
     8221 gcgacctgca tttccaggca gcgccagccg gagaacatac gttgtccgcc attcattatc
     8281 tgaccgaact gaacggctcg aaaaagcgca tcctggacaa tgcgcctgaa catattatta
     8341 ccggcccctg gaaacgcctg gtatacgatg cggagggccg gatacagcgt gccggttact
     8401 cgctttgtct gctggagcgc cttcaggatg cactacgtcg aagggacatc tggctcgaaa
     8461 acagcgatcg ctggggaaat ccccgcgaga agctgttgca gggggaagaa tggcaggctc
     8521 agcgggtccc cgtttgccgg gcgctgggac atcccactga tggacataaa ggcgtgcaac
     8581 agttggcggt ccagctagat gaaacctgga aaaccgtcgc atcccgcttt gaaggaaatg
     8641 cggaggttca tatttgccat gacggtaaac atccttccct gactatcagc agcctggaga
     8701 aactggagga gccaacatcg ttgcatcgtc tcaacagtcg ggtaaggctg ctactcccgc
     8761 cagtagattt gacggaactg ttgcttgaaa tagatgccag aacgggattt acacgtgagt
     8821 ttacgcatgt cagtgaatcc ggggcccggg cacaggatct gcacatcagc ctgtgcgcgg
     8881 tcctgatggc agaggcctgc aatatcgggc tggaaccgct gataaagcac aatataccgg
     8941 cgctgacgcg ccaccggctt agctgggtaa aacagaatta cctccgggca gaaacgctgg
     9001 tcagcgccaa tgcccgcctg gttgattttc agtccacact ggagcttgct ggccgctggg
     9061 gtggtggcga agtggcttca gctgatggca tgcgctttgt cacgccggtg aaaaccgtca
     9121 attcaggacc taacagaaaa tattttggtt ccggacgtgg catcacctgg tacaacttcg
     9181 tctctgatca gtactctgga ttccacggca tcgttgtccc cggcacatta cgagattcca
     9241 tttttgtgct ggaaggcctt ctggagcagc agacagggct gaatccggtt gagatcatga
     9301 cagacacagc cggtaccagc gacattattt ttggcctctt ctggctgctg ggataccagt
     9361 tttccccccg gcttgccgat gccggtgaag cggtattctg gcgagtggat aaatcggcaa
     9421 attacggtgc actggacgaa ctggcacgtg gttgtgccga tctgtcgaaa gccgaggatc
     9481 agtgggatga gatgatgcga accgccggtt cgctgaaact gggcaccatt catgcttcag
     9541 aactcattcg ctctttgctg aaaagctcgc gcccatcagg gctggcacag gcgatcatgg
     9601 aagtggggcg cgtcaacaag acgctgtacc ttcttaatta tattgatgat gaggattatc
     9661 gtcggcggat cctgacgcag ctaaaccggg gggaaggccg ccatgctgtg gcgagggcga
     9721 tctgctacgg gcagcgcggt gagatcagaa agcgctatcg tgaaggtcag gaagatcagc
     9781 tgggggcact gggcctggtc actaacgcag tggtactgtg gaacacactt tatatgcagg
     9841 aagccctgag ctggatacgc agtaatggag aagaaatcgg ggttgaagat atcgcccggt
     9901 tgtccccact gatgcacggg catatcaata tgctggggca ttatacgttt acgctaccgg
     9961 aggatatttt gaagggggaa ctgagagaac taaatttcaa tttaaacaat gaattaactt
    10021 cttagcgtac gttttcgttc cattggccct caaaccccat cttgtaacaa tcaggcatac
    10081 cacggagctt gtttgctt
//
LOCUS       DADLTY010000491         7053 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.guided.198, whole genome shotgun sequence.
ACCESSION   DADLTY010000491 DADLTY010000000
VERSION     DADLTY010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.guided.198"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            59..1102
                     /locus_tag="JN334_004599"
     CDS             59..1102
                     /locus_tag="JN334_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAY7440453.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            1218..1778
                     /locus_tag="JN334_004600"
                     /pseudo
     CDS             1218..1778
                     /locus_tag="JN334_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570718.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="JN334_004601"
     CDS             complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="JN334_004601"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY7440454.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3554..4012
                     /gene="gpt"
                     /locus_tag="JN334_004602"
     CDS             3554..4012
                     /gene="gpt"
                     /locus_tag="JN334_004602"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY7440455.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4104..5348
                     /gene="frsA"
                     /locus_tag="JN334_004603"
     CDS             4104..5348
                     /gene="frsA"
                     /locus_tag="JN334_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY7440456.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5406..5807
                     /locus_tag="JN334_004604"
     CDS             5406..5807
                     /locus_tag="JN334_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY7440457.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="JN334_004605"
     CDS             complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="JN334_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY7440458.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         1716 a         1735 c         1817 g         1785 t
ORIGIN      
        1 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
       61 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
      121 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
      181 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
      241 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
      301 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
      361 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
      421 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
      481 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
      541 gcatctgcaa ctgctggttc atagcggctc gagggggcta gggcagtcta ttttacagcg
      601 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
      661 tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg caacaggtta aggccaccgg
      721 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
      781 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
      841 ttcacgcggt gattactcca ggctggttca gcccgtcagg agtgaggaaa cattgcattc
      901 gctggcgcat ggggctgggc gtaaatgggg gcgcaccgag tgtaaagggc gtctggcagc
      961 gaaatacaca gcgacgcaac tctcgcgtac tgaacttggc agtcgggtaa tttgtcgcga
     1021 taaacaactc atcttcgaag aagcgccaca agcctataaa tcggctgaaa gtgtggtgca
     1081 atgtcagggc aggccggatt aattattcct gtcgcgcggc tgcgtccggt gctaacgctc
     1141 aaaaacagtg gagggaaaaa aggatgatct tgctataact ctcctcagcg caggggccgg
     1201 aagaatgttg tctcgcagtg aaaaaggcgc tggacaggct aattaaagaa gctgcccgac
     1261 aagacgtcgc ggtaacggtg ctggaaacag aaacaggccg ctactctgac acgctgcgtt
     1321 cggcgctgat ttctctggat ggcgacaacg catgggcgtt aagcgaaagc tggtgtggca
     1381 ctattcagtg gatttgcccg agcccgtatc gcccacatca cgggcgtaaa aactggtttc
     1441 tgggcattgg gcgttttacc gctgatgagc aggagcaatc cgatgcgatc cggtatgaga
     1501 cgctgcgttc gtcggggccg ggcggtcaac atgtcaataa aaccgactcg gcggtacgcg
     1561 ccacgcattt ggcatccggt attagcgtga aggttcagtc agagcgcagc cagcatgcta
     1621 acaaacggct ggcgcgattg ctgattgcct ggaagctgga tcagcagcaa taggaaaata
     1681 gcgcggtgct gaaatcacag cggcgaatgt tccatcacca gattgaacgt ggcaacccgc
     1741 gacgaacgtt tacagggatg gcttttatcg aaggataatg aaggatgaaa ctgccggaag
     1801 gcgattaaac gccatccggc agcaaatcaa ataattactt cgccggaatt tctttcagca
     1861 gttcagtcag cagtgtccag taatgaccta cgctttcgat gtgaacttgc tcatccggag
     1921 agtgtggacc ggtgatagtt ggcccgatag aaaccatgtc catttccgga tacggttttt
     1981 tgaacagacc acattccagg cccgcgtgga taatctggat gttcggcgtc ttgttgaaca
     2041 ggcgctgata ggtttcacgt accagatgca tcaccggaga attagcgtcc ggctgccagc
     2101 caggatatgc gcctttcgct tcggttttcg cgccagccag tttacccagc gaatccagca
     2161 tgctcaccac gtagtcttta ccgctgtcga ttagtgaacg gatcaggcag tgaatttcta
     2221 cgttattgtc agtcatggtc accacaccga cgttcaggga ggtttcgacc acaccttttg
     2281 ccacgtcgga attgcggatc acaccgttcg gggtggcgtt cagcagacga ataaaggtat
     2341 cgcgagattt cgcaatcagg gcagctttat cgttcgctac agagtccagc aacaaggcca
     2401 gatttttctc tttctctgcc agctcgtttt tcaggatctc ctgataggta ttcaccagag
     2461 atttcagtgc gtcgacttta tcagctgcga cagcaatggt cgcaaaggct tcacgcggga
     2521 tggcgttacg cagtgtgccg ccgttgaaat cgataaggcg caggtccagt tcttctgcat
     2581 gacccgccag gaagcgcacc agcagtttgt tggcattacc caggccaacg tggatttcac
     2641 cgccggagtg accgcctttc agacctttta aggttaactt gaaggtttca aaaccagctg
     2701 gaaccgcttc acgatctaaa tgcaggttgg aggtgaagtc gatacccccc gcacaaccca
     2761 tgtagatttc accttcttct tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt
     2821 tgctctgtaa gccgaacgca ccgtccatac cggcttcttc ggtcatggtc agcagcactt
     2881 ccagcgggcc gtgaaccacg ttttcgtcag ccagaaccgc cagtgcagag gccataccaa
     2941 tgccgttatc cgcacccagc gtggtgccgc gcgctttaac ccattcgcca tcaatataag
     3001 gctggatagg atctttcgtg aagtcatgca cggtgtcgtt atttttctgc ggcaccatat
     3061 cgaggtgggc ctgtaagacg accggtttac gattttccat acctgcggta gcaggttgac
     3121 gaatcaggat attacctacc tgatcgcgtt cgacatggaa acctttctct tttgcccaac
     3181 caacaatgta ttcagcgagt tgctcttcat gataggacgg gtgaggaata gaacagattt
     3241 tggcaaaaat atcccacagc ggctgtggag ataattgaga cagttcagac acgttaagtc
     3301 tccttgtcga tcacccgcaa aacagtattg caggtcacag ggttagcaga aaatgttgtc
     3361 aacacaagac aggcttgcga gatatgtatt gagaatacca ctttatcccg cgtcagggag
     3421 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
     3481 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
     3541 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
     3601 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
     3661 ccgtggcggt ctggtaccgg gggcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     3721 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     3781 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     3841 tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     3901 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggatcga
     3961 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     4021 acgcctggca ttgccgggcg ttgttctttt taacttcagg caggttacaa tagtttccag
     4081 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     4141 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     4201 cgcctgtgca gtcggctctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     4261 gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat cctcgacgtc caggcacgta
     4321 ttgtgatgag cgatgctgaa cgtaccgacg atgatttata cgataccgtg attggctacc
     4381 gtggtggcaa ctggatttat gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct
     4441 gtacggaaga agatccgcaa ctcagtggtc gacactggct gcatgcggct acgttgtaca
     4501 acattgccgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttat
     4561 caaaccgcgc ctatgaagag gccgcccagc gtctgccggg cacgatgcgg cagatggagt
     4621 ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc
     4681 cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc
     4741 tgtatgaacg ttattttgcg ccgcgcggca ttgcgatgct gactattgat atgccgtcgg
     4801 tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgtct
     4861 taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc
     4921 gtttcggcgc taacgttgcc gtgcgtctgg cataccttga atcgccgcgt ctgaaagcgg
     4981 ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg
     5041 tgccggaaat gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg
     5101 aagcgttacg cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctggggcgtc
     5161 gctgcccaac gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg
     5221 actcacgctt aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc
     5281 cggtgtatcg gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct
     5341 tgtgttaaaa atttgctaaa ttttgccaat ttggtaaaac agttgcatca caacaggaga
     5401 tagcaatgac gttaccgagt ggacacccga agagcagatt gatcaaaaaa tttaccgcac
     5461 taggcccgta tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg
     5521 ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga attctggggc tggtggatgg
     5581 agctggaagc gcaggaatcc cgttttacct acagctacca gtttggtctg ttcgataaag
     5641 caggcgactg gaagagtgtt ccggtaaaag atactgaagt ggttgaacga ctggagcaca
     5701 ccctgcgtga gtttcacgag aagctgcgtg aactgctgac gacgctgaat ctgaagctgg
     5761 aaccggcgaa tgattttcgt gatgaaccgg tgaagttaac ggcgtgagtg aaatgtgccg
     5821 gatgcatcac atccggcaat attcattaaa actgatacgt catcccaacc gcgacagtat
     5881 catcattatt aatattcagc ttattatcgc tatccagttg gttgatttta taatcaacaa
     5941 acgctgacat atttttgttg aaataatacg tagcaccgac gtcgatataa ttgaccagat
     6001 cttcatcacc gatgccctca atatctttcc ctttcgataa gacataaccc agcgatggac
     6061 gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa
     6121 agccgccagt tattggcgtc attttgcgtg tttcagaata gaaagttgcc agataaatat
     6181 tattggcatc gtatttcaga cctgttgccc atgcttctgc acgcttgcct gtgccacggc
     6241 tttgcaggtt ctgctcgttg gtgcgatctg agttggtata ggccccacta atggcgaaat
     6301 cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa
     6361 cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa gttcaggcca tcgataacgc
     6421 cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc gcgtttggtc ataaagttgt
     6481 cggtctgcgc cgaggaatcg ccaccaaatt ccgggaacat atcggtccag gcttccacgt
     6541 catacaacgc gcccaggtta cgaccataat cgaaagaacc caaatcttta tatttcaacc
     6601 cggcaaaagc gagacgcgtt ttttgctgtg cagtatcact ctccgcttta tttccggcaa
     6661 actccgcttc ccaccgacca taaccagtca gttgatcgtt aatttgagtt tcacctttaa
     6721 aaccaaaacg gatataactc tggtcgccat ctttactggc gttatcactc atataatgca
     6781 tggctttaac tttgccatag acatccagtt tattaccgtc tttattatat atttctgcag
     6841 cctgtacaga tgcagatgcc acaatgccca tcaccactaa tgccagagtg ctctttttca
     6901 ttttcattcc tgattttaat taacgcgcga atattcagcg ggagagcccc gttgaaaaca
     6961 ggaaagtttt taacctgaga ttgttaaaga tatattacag attaatgata ttctgaaaat
     7021 gggtaatgct gccaacttac tgatttagtg tat
//
LOCUS       DADLTY010000492         5717 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.guided.228, whole genome shotgun sequence.
ACCESSION   DADLTY010000492 DADLTY010000000
VERSION     DADLTY010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.guided.228"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            <1..80
                     /locus_tag="JN334_004606"
     CDS             <1..80
                     /locus_tag="JN334_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY7440459.1"
                     /translation="SLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            77..340
                     /locus_tag="JN334_004607"
     CDS             77..340
                     /locus_tag="JN334_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY7440460.1"
                     /translation="MSWSHYRPLLSHVAGFISIERFQSLATEGKMLSLSWWENEYAVL
                     QWKNHVLHAKAQQEGRESIFDFYKISIAHITREYSFKKDKDNV"
     gene            333..719
                     /locus_tag="JN334_004608"
     CDS             333..719
                     /locus_tag="JN334_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HAY7440461.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            788..1411
                     /locus_tag="JN334_004609"
     CDS             788..1411
                     /locus_tag="JN334_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY7440462.1"
                     /translation="MSDISRVKILSALMDGRAWTATELSSVANISASTASSHLSKLLD
                     CQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHGVHAKVSTPVHLRKARTCYDHL
                     AGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSER
                     RFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(1389..2012)
                     /gene="tetR(B)"
                     /locus_tag="JN334_004610"
     CDS             complement(1389..2012)
                     /gene="tetR(B)"
                     /locus_tag="JN334_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HAY7440463.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2094..3299
                     /gene="tet(B)"
                     /locus_tag="JN334_004611"
     CDS             2094..3299
                     /gene="tet(B)"
                     /locus_tag="JN334_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HAY7440464.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3412..4005)
                     /gene="tetC"
                     /locus_tag="JN334_004612"
     CDS             complement(3412..4005)
                     /gene="tetC"
                     /locus_tag="JN334_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HAY7440465.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4093..4503
                     /locus_tag="JN334_004613"
                     /pseudo
     CDS             4093..4503
                     /locus_tag="JN334_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4519..>5717)
                     /locus_tag="JN334_004614"
     CDS             complement(4519..>5717)
                     /locus_tag="JN334_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HAY7440466.1"
                     /translation="HKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRNL
                     PTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKR
                     LMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGF
                     KVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKS
                     NPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQ
                     LVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVH
                     AQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHG
                     YALGKL"
BASE COUNT         1581 a         1056 c         1244 g         1836 t
ORIGIN      
        1 agtcgctgtc gttctcaaaa tcggtggagc tgcatgacaa agtcatcggg cattatctga
       61 acataaaaca ctatcaataa gttggagtca ttaccgacca ctattaagtc atgtagctgg
      121 ttttatttca attgaacgtt ttcaaagtct agctacagaa ggaaaaatgt tatcgctatc
      181 ttggtgggaa aacgaatacg cagttctgca atggaaaaat catgttttac atgcgaaagc
      241 tcaacaagaa gggcgagagt caatatttga tttttacaaa attagtattg ctcatattac
      301 tcgcgaatat tcatttaaaa aggacaagga taatgtttga tgttcacgtt gttttagata
      361 atcaaatagg acaattagca ttactaggaa aaacattagg taataaaggt attggattgg
      421 aagggggagg gatatttacg gttggtgatg aatgccatgc tcattttctt gttgaacaag
      481 gaaaggaagc taaaatagcg ctagagcaag ctggactgtt agtacttgcg atccggacac
      541 cattaattcg taagttaaaa caggaaaaac cgggggaact tggcgaaata gcacgagtat
      601 tggcggagaa taacattaat attttagtgc aatacagtga ccatgctaac caactgatat
      661 taataacgga caatgatagt atggctgcat ctgttacgct cccttgggca ataaagtgaa
      721 cttgcgatgg ctaatttaat acgaaaagag gttacctttg agtcctcaat agccgctagg
      781 ggcggctatg tctgacattt cacgagttaa aatactcagt gctttgatgg atgggcgagc
      841 ttggacggcc actgagctaa gttctgtggc gaatatatca gcttcaacgg cgagcagtca
      901 tttatctaaa ttattagatt gccagctaat cacagtagta gctcaaggca agcatcgtta
      961 ttttcggcta gcaggaaaag atattgctga attgatggaa agtatgatgg ggatctcctt
     1021 aaaccatggc gtacatgcca aagtttccac gccagtgcat ttacgaaaag cacgtacttg
     1081 ctatgatcat ttagctggcg aagttgccgt taagatctat gattcccttt gtcaacagca
     1141 atggatcact gaaaatggtt caatgatcac attaagtggt attcaatatt ttcatgaaat
     1201 gggaattgac gttccttcca aacattcacg taaaatctgt tgtgcgtgtt tagattggag
     1261 tgaacgccgt ttccatttag gtgggtacgt tggagccgca ttattttcgc tttatgaatc
     1321 taaagggtgg ttaactcgac atcttggtta ccgtgaagtt accatcacgg aaaaaggtta
     1381 tgctgctttt aagacccact ttcacattta agttgttttt ctaatccgca tatgatcaat
     1441 tcaaggccga ataagaaggc tggctctgca ccttggtgat caaataattc gatagcttgt
     1501 cgtaataatg gcggcatact atcagtagta ggtgtttccc tttcttcttt agcgacttga
     1561 tgctcttgat cttccaatac gcaacctaaa gtaaaatgcc ccacagcgct gagtgcatat
     1621 aatgcattct ctagtgaaaa accttgttgg cataaaaagg ctaattgatt ttcgagagtt
     1681 tcatactgtt tttctgtagg ccgtgtacct aaatgtactt ttgctccatc gcgatgactt
     1741 agtaaagcac atctaaaact tttagcgtta ttacgtaaaa aatcttgcca gctttcccct
     1801 tctaaagggc aaaagtgagt atggtgccta tctaacatct caatggctaa ggcgtcgagc
     1861 aaagcccgct tattttttac atgccaatac aatgtaggct gctctacacc tagcttctgg
     1921 gcgagtttac gggttgttaa accttcgatt ccgacctcat taagcagctc taatgcgctg
     1981 ttaatcactt tacttttatc taatctagac atcattaatt cctaattttt gttgacactc
     2041 tatcattgat agagttattt taccactccc tatcagtgat agagaaaagt gaaatgaata
     2101 gttcgacaaa gatcgcattg gtaattacgt tactcgatgc catggggatt ggccttatca
     2161 tgccagtctt gccaacgtta ttacgtgaat ttattgcttc ggaagatatc gctaaccact
     2221 ttggcgtatt gcttgcactt tatgcgttaa tgcaggttat ctttgctcct tggcttggaa
     2281 aaatgtctga ccgatttggt cggcgcccag tgctgttgtt gtcattaata ggcgcatcgc
     2341 tggattactt attgctggct ttttcaagtg cgctttggat gctgtattta ggccgtttgc
     2401 tttcagggat cacaggagct actggggctg tcgcggcatc ggtcattgcc gataccacct
     2461 cagcttctca acgcgtgaag tggttcggtt ggttaggggc aagttttggg cttggtttaa
     2521 tagcggggcc tattattggt ggttttgcag gagagatttc accgcatagt ccctttttta
     2581 tcgctgcgtt gctaaatatt gtcgctttcc ttgtggttat gttttggttc cgtgaaacca
     2641 aaaatacacg tgataataca gataccgaag taggggttga gacgcaatcg aattcggtat
     2701 acatcacttt atttaaaacg atgcccattt tgttgattat ttatttttca gcgcaattga
     2761 taggccaaat tcccgcaacg gtgtgggtgc tatttaccga aaatcgtttt ggatggaata
     2821 gcatgatggt tggcttttca ttagcgggtc ttggtctttt acactcagta ttccaagcct
     2881 ttgtggcagg aagaatagcc actaaatggg gcgaaaaaac ggcagtactg ctcggattta
     2941 ttgcagatag tagtgcattt gcctttttag cgtttatatc tgaaggttgg ttagttttcc
     3001 ctgttttaat tttattggct ggtggtggga tcgctttacc tgcattacag ggagtgatgt
     3061 ctatccaaac aaagagtcat cagcaaggtg ctttacaggg attattggtg agccttacca
     3121 atgcaaccgg tgttattggc ccattactgt ttgctgttat ttataatcat tcactaccaa
     3181 tttgggatgg ctggatttgg attattggtt tagcgtttta ctgtattatt atcctgctat
     3241 cgatgacctt catgttaacc cctcaagctc aggggagtaa acaggagaca agtgcttagt
     3301 tatttcgtca ccaaatgatg ttattccgcg aaatataatg accctcttga taacccaaga
     3361 gggcattttt tacgataaag aagatttagc ttcaaataaa acctatctat tttatttatc
     3421 tttcaagctc aataaaaagc cgcggtaaat agcaataaat tggccttttt tatcggcaag
     3481 ctcttttagg tttttcgcat gtattgcgat atgcataaac cagccattga gtaagttttt
     3541 aagcacatca tcatcataag ctttaagttg gttctcttgg atcaatttgc tgacaatggc
     3601 gtttacctta ccagtaatgt attcaaggct aattttttca agttcattcc aaccaatgat
     3661 aggcatcact tcttggatag ggataaggtt tttattatta tcaataatat aatcaagata
     3721 atgttcaaat atactttcta aggcagacca accatttgtt aaatcagttt ttgttgtgat
     3781 gtaggcatca atcataatta attgctgctt ataacaggca ctgagtaatt gttttttatt
     3841 tttaaagtga tgataaaagg cacctttggt caccaacgct tttcccgaga tctcatctat
     3901 tgaaacagct tgatagcctt tttcaacaaa caatattcgt gctgagttaa ccagtgattg
     3961 ataggtactc ttaaaatttt cttgttgatg atttttattt tccatgatag atttaaaata
     4021 acataccgtc agtatgttta tggtatcatg atgatgtggt cgtgacaatc ttaagaacat
     4081 ttaggttatt ttatgtatat tgaacagcat tctcgctatc aaaataaagc taataacatc
     4141 caattaagat atgatgataa gcagtttcat acaacggtta tcaaagatgt tctattatgg
     4201 attgaacata atttagatca gtctttactg cttgatgatg tggcgaataa agcgggttat
     4261 accaagtggt attttcagcg gctgttcaaa aaagtaacag gggtcacact ggctagctat
     4321 attcgtgctc gtcgtttgac gaaagcggct gttgagttga ggttgacgaa aaaaactatc
     4381 cttgagatcg cattaaaata tcaatttgat tcccaacaat cttttacacg tcgatttaag
     4441 tacattttta aggttacacc aagttattat cggcgtaata aattatggga attggaggca
     4501 atgcactgag agatcccctc ataatttccc caaagcgtaa ccatgtgtga ataaattttg
     4561 agctagtagg gttgcagcca cgagtaagtc ttcccttgtt attgtgtagc cagaatgccg
     4621 caaaacttcc atgcctaagc gaactgttga gagtacgttt cgatttctga ctgtgttagc
     4681 ctggaagtgc ttgtcccaac cttgtttctg agcatgaacg cccgcaagcc aacatgttag
     4741 ttgaagcatc agggcgatta gcagcatgat atcaaaacgc tctgagctgc tcgttcggct
     4801 atggcgtagg cctagtccgt aggcaggact tttcaagtct cggaaggttt cttcaatctg
     4861 cattcgcttc gaatagatat taacaagttg tttgggtgtt cgaatttcaa caggtaagtt
     4921 agttgctaga acccatggct cctttgccga cgctgagtag attttaggtg acgggtggtg
     4981 acaatgagtc cgtgtcgagc gctgattttt tcggccttta gagcgagatt tatacaatag
     5041 aatttggcat gagattggat tgcttttagt cagcctctta tagcctaaag tctttgagtg
     5101 actagatgac atatcatgta agttgctgat aggtttccag ttttccgctc ctaggtctgc
     5161 atattgtact tttcctctta ctcgacttaa ccagtaccaa cccagcttct caacggattt
     5221 ataccatggc actttaaagc cagcatcact gacaatgagc ggtgtggtgt tactcggtag
     5281 aatgctcgca aggtcggcta gaaattggtc atgagctttc tttgaacatt gctctgaaag
     5341 cgggaacgct ttctcataaa gagtaacaga acgaccgtgt agtgcgactg aagctcgcaa
     5401 taccataagt cgtttttgct cacgaatatc agaccagtca acaagtacaa tgggcatcgt
     5461 attgcccgaa cagataaagc tagcatgcca acggtataca gcgagtcgct ctttgtggag
     5521 gtgacgatta cctaacaatc ggtcgattcg tttgatgtta tgttttgttc tcgctttggt
     5581 tggcaggtta cggccaagtt cggtaagagt gagagtttta cagtcaagta atgcgtggca
     5641 agccaacgtt aagctgttga gtcgttttaa gtgtaattcg gggtaatgac tccaacttac
     5701 tgatagtgtt ttatgtt
//
LOCUS       DADLTY010000493         4645 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.guided.253, whole genome shotgun sequence.
ACCESSION   DADLTY010000493 DADLTY010000000
VERSION     DADLTY010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.guided.253"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            32..1165
                     /gene="blaEC"
                     /locus_tag="JN334_004615"
     CDS             32..1165
                     /gene="blaEC"
                     /locus_tag="JN334_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY7440467.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1254..1787
                     /locus_tag="JN334_004616"
     CDS             1254..1787
                     /locus_tag="JN334_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY7440468.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1784..2101)
                     /locus_tag="JN334_004617"
     CDS             complement(1784..2101)
                     /locus_tag="JN334_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAY7440469.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2277..2423)
                     /gene="ecnB"
                     /locus_tag="JN334_004618"
     CDS             complement(2277..2423)
                     /gene="ecnB"
                     /locus_tag="JN334_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY7440470.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2534..2659)
                     /gene="ecnA"
                     /locus_tag="JN334_004619"
     CDS             complement(2534..2659)
                     /gene="ecnA"
                     /locus_tag="JN334_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY7440471.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(2711..3277)
                     /gene="efp"
                     /locus_tag="JN334_004620"
     CDS             complement(2711..3277)
                     /gene="efp"
                     /locus_tag="JN334_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY7440472.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3319..4347
                     /gene="epmB"
                     /locus_tag="JN334_004621"
     CDS             3319..4347
                     /gene="epmB"
                     /locus_tag="JN334_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY7440473.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1193 a         1176 c         1152 g         1124 t
ORIGIN      
        1 ggcgggccgt tttgtatgga aaccagacct tatgttcaaa acgacgctct gcgccttatt
       61 aattaccgcc tcttactcca catttgctgc ccctcaacaa atcaacgata ttgtgcatcg
      121 cacaattacc ccgcttatag agcaacaaaa gatccccggt atggcggtgg cggtaattta
      181 tcagggtaaa ccttattact ttacctgggg ctatgcggac atcgccaaaa agcagcccgt
      241 cacacagcaa acgttgtttg agttaggttc ggtcagcaaa acatttacgg gcgtgcttgg
      301 tggcgacgct attgctcgag gggaaatcaa gttaagcgat cccgcaacaa aatactggcc
      361 tgaacttacc gctaaacagt ggaatgggat cacactatta catcttgcga cctacaccgc
      421 tggcggcctg ccattgcagg tgccggatga agtgaaatcc tcaagcgact tgctgcgctt
      481 ctatcaaaac tggcagcctg catgggctcc aggaacacaa cgtctgtatg ccaactccag
      541 tatcggtttg ttcggcgcac tggctgtgaa gccgtctggt ttgagttttg agcaggcgat
      601 gcaaactcgt gtcttccagc cactcaaact caaccatacg tggattaatg tacctccccc
      661 agaagaaaag aattacgcct ggggatatcg cgaaggtaag gcagtgcatg tttcgccagg
      721 ggcgttagat gctgaagctt atggtgtgaa gtcgaccatt gaagatatgg cccgctgggt
      781 gcaaagcaat ttaccccccc ttgatatcaa tgagaaaacg cttcaacaag ggatacaact
      841 ggcacaatct cgctactggc aaaccggcga tatgtatcag ggcctgggct gggaaatgct
      901 ggactggccg gtcaatcctg acagcatcat taacggcagt gacaataaaa ttgcactggc
      961 agcacgcccg gtaaaagcga ttaagccccc aactcctgca gtacgcgcat catgggtaca
     1021 taaaacgggg gcgaccggcg gatttggtag ctatgtcgcg tttattccag aaaaagagct
     1081 gggtatcgtg atgctggcta acaaaaacta tcccaatcca gcgagagtcg ccgccgcctg
     1141 tcagattctc aacgctctac agtaaaattt catcgggtcc gaattttcgg accttttctg
     1201 cgcttttcct tgctgtcatc tacacttaga aaaaaaccag taaggaaaca tttatgcgcc
     1261 tgctccctct cgttgccgca gcgacagctg catttctggt cgttgcctgt agttttccta
     1321 cgccgccgcg tggcgtgacc gtagtgaata atttcgacgc caaacgttat ctcggtacct
     1381 ggtatgagat tgcccgtttt gatcaccgtt ttgaacgtgg actggaaaaa gtcaccgcaa
     1441 catacagcct gcgtgatgac ggcggcctga atgtcattaa taaaggctat aaccctgaca
     1501 gaggaatgtg gcagcagagt gaagggaaag cgtactttac cggcgcacca actcgcgctg
     1561 cgctgaaagt gtcattcttt ggtcctttct atggcggtta taacgttatt gcactcgatc
     1621 gggaataccg ccatgcgctg gtttgcggcc cggaccgcga ctacctgtgg atactctccc
     1681 gcacgccaac catttctgac gaagtgaaac aggagatgct ggcagtcgcg acccgggaag
     1741 ggtttgatgt cagtaagttt atttgggtac agcagcccgg tagttagtga gtgctgagtt
     1801 tcagaccaat aatccccaat acgattaacg ccagactcgc cagacgcatc gggttagcgg
     1861 actcaccgag cagcacaatg ccggtgatgg ccgcgccgac tgcgccaata cccgtccaca
     1921 cggcataagc cgtccctacc ggtaacgatt tcatcgccca ggcaagtagc gccatactga
     1981 ctatcatcgc cgtcacggta ataacactcg gcgtcaaacg gctaaagccg tgggtatatt
     2041 tcaggccaac ggcccatacc acttccagca gaccagcaat aactaagata atccaggaca
     2101 tatcaggctc cggaaatttg gggccgtccc cggtgaaaag aggcgtttgc aggtcgtcct
     2161 gcaaagctga tggttgaacg gatattttgc caggtggcag gggaatttca actattacct
     2221 ttatgacgat cgcagaaaaa acaaacggca cgacactgct gtcgtaccgt tattgcttat
     2281 tgctgcgctt tcgttgctgc gccagaaatc gcgttaccgc catcagaaat gtcttcacca
     2341 acgccacgcg tggtgttgca ggcagttaat actgttgaaa gcaccagaac agaaaagatc
     2401 gctgcaattg tcttcttcac cataacgtct tccttttagg taatgttatt tatgtttgcc
     2461 tatagcaaat taataataga caacatcgcc aaaaatgatg gaaatctgat gatttttcgg
     2521 aagagaagaa aaattagctg gcagcgcgag agatggagtt accgagatgt ttgatgtctt
     2581 cgccgaaacc gcaagcggtg ttacagcccg tgagcagcgt gctggcaagc aaaacaagaa
     2641 cgataaggcg tttcatcatc tctgacctga ataagtgatg gtgcagcctg caggccgcac
     2701 cacaaccgca ttacttcacg cgagagacgt attcaccaga gcgggtatcc actttgatga
     2761 cttcgccgat ttgtacgaac agcggaactt taaccacagc gccagtagac agggtagccg
     2821 gtttgccgcc agtacctgcg gtatcacctt tcaggcccgg atcggtatca acgatttcca
     2881 gttcaacgaa gttcggcgga gtaacggaga tcggctgacc attccacaga gttacgatac
     2941 actctgcctg atccagcagc catttagcat tgtcgccaat tgcttttgca tcagcagaca
     3001 gctgctcgaa agtttcgttg ttcatgaagt gccagaactc accgtcgttg tacaggtaag
     3061 tcaggttcat atcgacaaca tcagcgcctt cagcggaatc agtagatttg aaggtttttt
     3121 ctacgcgagt accggtcagc agacgacgca gtttaacgcg agcaaatgcc tggcctttac
     3181 ccggttttac gaattcactc gcttcaaccg cgtaaggttc gccgtctaac atgattttaa
     3241 gaccagcacg aaaatcgttg ctatagtacg ttgccataag gccctctgaa atttgttaat
     3301 tggtagctaa gccacaaaat ggcgcatatt gtaaccctaa ataccccatc cagagaagat
     3361 tggttaacgc aacttgccga tgttgtgacc gatcctgatg aacttctgcg tcttttgaat
     3421 atagacgcgg acgaaaaact gttggccgga cgcagcgcca aaaagctgtt tgccctgcgt
     3481 gtgccccgct catttatcga tcgcatggag aaaggcaatc cggacgatcc tcttttgcgt
     3541 caggtactta cctcgcaaga tgagtttgtc gtcgcgtccg gattctccac cgacccactg
     3601 gaagaacagc acagcgtagt gcctggtttg ttgcataaat accacaaccg ggcgcttttg
     3661 ctggtcaaag gcggctgcgc ggtaaattgc cgctattgct tccgtcgcca cttcccctat
     3721 gccgaaaatc agggcaacaa gcgtaactgg cagactgcac ttgagtatgt tgctgcgcat
     3781 ccggaactgg acgagatgat tttctccggc ggcgatccgc tgatggcgaa agagcacgag
     3841 ctggactggt tgctcacaca actggaagcc atcccgcata tcaaacgtct gcggattcac
     3901 agccgtctgc cgattgtgat cccggcacgt atcaccgagg cgctggttga acgctttgcc
     3961 cgttctacgc tgcaaatctt gctggtgaat cacatcaatc atgccaatga ggtagatgaa
     4021 acgttccgtc aggcgatggc taaattgcgc cgtgtcggtg tcaccctgct taaccagagc
     4081 gttctgttac gtggtgtgaa cgataacgca caaacgttgg caaacctgag taatgcgttg
     4141 ttcgatgccg gcgtgatgcc gtattacctg catgtgctag ataaagtaca gggcgcggcg
     4201 cattttatgg tgagtgatga cgaagcacgg cagattatgc gtgagttgct gacattggtg
     4261 tcgggatatc tggtgccgaa actggcgcga gaaattggcg gcgaacccag caaaacgccg
     4321 ctggatctcc agctacgcca gcagtaactg attaaacagg gaagttaaac attaaaattg
     4381 cctgatgcgc tacgcttatc aggcctacat cgtgcattga aattgaatgg atttgcaatg
     4441 aactgtaggc cggataaggc gtttacgccg catccggcaa gagcaacaaa cgcaagtaca
     4501 gcaaccactg agaaatttaa tctttgcacg ttgcataacc tttaattatt catatgcatt
     4561 gctgttgtta ctttttatat agctaacacc aataattaac ctttcttttc ggtaatgact
     4621 ccaacttact gatagtgttt tatgt
//
LOCUS       DADLTY010000494         1962 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.guided.354, whole genome shotgun sequence.
ACCESSION   DADLTY010000494 DADLTY010000000
VERSION     DADLTY010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.guided.354"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            109..939
                     /locus_tag="JN334_004622"
     CDS             109..939
                     /locus_tag="JN334_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HAY7440474.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1052..1843
                     /gene="aadA1"
                     /locus_tag="JN334_004623"
     CDS             1052..1843
                     /gene="aadA1"
                     /locus_tag="JN334_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY7440475.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          614 a          433 c          435 g          480 t
ORIGIN      
        1 acgatgttac gcagcagggc agtcgcccta aaacaaagtt gggcgaaccc ggagcctcat
       61 taattgttag ccgttaaaat taagcccttt accaaaccaa tacttattat gaaaaacaca
      121 atacatatca acttcgctat ttttttaata attgcaaata ttatctacag cagcgccagt
      181 gcatcaacag atatctctac tgttgcatct ccattatttg aaggaactga aggttgtttt
      241 ttactttacg atgcatccac aaacgctgaa attgctcaat tcaataaagc aaagtgtgca
      301 acgcaaatgg caccagattc aactttcaag atcgcattat cacttatggc atttgatgcg
      361 gaaataatag atcagaaaac catattcaaa tgggataaaa cccccaaagg aatggagatc
      421 tggaacagca atcatacacc aaagacgtgg atgcaatttt ctgttgtttg ggtttcgcaa
      481 gaaataaccc aaaaaattgg attaaataaa atcaagaatt atctcaaaga ttttgattat
      541 ggaaatcaag acttctctgg agataaagaa agaaacaacg gattaacaga agcatggctc
      601 gaaagtagct taaaaatttc accagaagaa caaattcaat tcctgcgtaa aattattaat
      661 cacaatctcc cagttaaaaa ctcagccata gaaaacacca tagagaacat gtatctacaa
      721 gatctggata atagtacaaa actgtatggg aaaactggtg caggattcac agcaaataga
      781 accttacaaa acggatggtt tgaagggttt attataagca aatcaggaca taaatatgtt
      841 tttgtgtccg cacttacagg aaacttgggg tcgaatttaa catcaagcat aaaagccaag
      901 aaaaatgcga tcaccattct aaacacacta aatttataaa aaatctaatg gcaaaatcgc
      961 ccaacccttc aatcaagtcg ggacggccaa aagcaagctt ttggctcccc tcgctggcgc
     1021 tcggcgcccc ttatttcaaa cgttaaacat catgagggaa gtggtgatcg ccgaagtatc
     1081 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     1141 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
     1201 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     1261 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     1321 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     1381 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     1441 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     1501 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     1561 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     1621 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     1681 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     1741 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     1801 tcactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc
     1861 aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat
     1921 tgcggtgatg ctgccaactt actgatttag tgtatgatgg tg
//
LOCUS       DADLTY010000495         1812 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.guided.364, whole genome shotgun sequence.
ACCESSION   DADLTY010000495 DADLTY010000000
VERSION     DADLTY010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.guided.364"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            264..923
                     /locus_tag="JN334_004624"
     CDS             264..923
                     /locus_tag="JN334_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HAY7440476.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1124..1501)
                     /locus_tag="JN334_004625"
     CDS             complement(1124..1501)
                     /locus_tag="JN334_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7440477.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1568..>1812)
                     /locus_tag="JN334_004626"
     CDS             complement(1568..>1812)
                     /locus_tag="JN334_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7440478.1"
                     /translation="RYRASGLNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYL
                     SPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT          468 a          431 c          445 g          468 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1201 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1261 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgct ttacccgtgc
     1321 ccttatttcg atcgcactga gaaacataca gctctttaat atgcagctga ccgctgtatc
     1381 ggggcgaggg ataaagaata ttgcaacatg ccaggccagt aatgttattg ccgcagcgcg
     1441 ctctgatcac cagggtaccg gaaagccggt tgaataactt tttacagaga taatccttca
     1501 tcaacgcttc ctgaatgata ccttcgccat aataggggtc gtctcagaaa acggaaaata
     1561 aagcacgcta agccggttgc agaggccgta gcggcctgaa cttccccgcg ccgatcttgg
     1621 cgctgctgcg ccataggtaa tcaccggtca ggttgatgtg ctcccagccg agtggcgaca
     1681 ggtactgcaa tagcgagtca tcgacggcat gaccattgcc gcgcaacgca tgcgccgcac
     1741 gctccaggta gaccgtgttc cacagcacga tggccgccgt caccaggttg aggccgctgg
     1801 cccggtagcg ct
//
LOCUS       DADLTY010000496          751 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain DE0614
            SAMEA1029914-rid11415893.guided.422, whole genome shotgun sequence.
ACCESSION   DADLTY010000496 DADLTY010000000
VERSION     DADLTY010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029914
            Sequence Read Archive: ERR047367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942057.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,627
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,190
            CDSs (with protein)               :: 4,190
            Genes (RNA)                       :: 53
            rRNAs                             :: 3, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 181 of 384
            Pseudo Genes (incomplete)         :: 95 of 384
            Pseudo Genes (internal stop)      :: 146 of 384
            Pseudo Genes (multiple problems)  :: 35 of 384
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029914-rid11415893.guided.422"
                     /strain="DE0614"
                     /db_xref="taxon:623"
     gene            58..531
                     /locus_tag="JN334_004627"
     CDS             58..531
                     /locus_tag="JN334_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HAY7440479.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
BASE COUNT          219 a          159 c          182 g          191 t
ORIGIN      
        1 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aacccaggat gagaaccttg
       61 aaagtatcat tgatggctgc gaaagcgaaa aacggcgtga ttggttgcgg tccagacata
      121 ccctggtccg cgaaagggga gcagctactt tttaaagcat tgacctacaa tcagtggctt
      181 ctggtgggtc gcaagacgtt tgaatctatg ggcgcactcc ccaataggaa atacgcggtc
      241 gttacccgct caggttggac atcaaatgat gacaatgtag ttgtatttca gtcaatcgaa
      301 gaggccatgg acaggctagc tgaattcacc ggtcacgtta tagtgtctgg tggcggagaa
      361 atttaccgag aaacattacc catggcctct acgctccact tatcgacgat cgacatcgag
      421 ccagaggggg atgttttctt cccgagtatt ccaaatacct tcgaagttgt ttttgagcaa
      481 cactttactt caaacattaa ctattgctat caaatttgga aaaagggtta acaaagctat
      541 gcaatcgacg gcaaaaagct tcgttcgctt cgcgcactac gccttttccg cgattgatag
      601 cgacgttatg tgaatattga aatgagcatt ccaaagaaag gaagtagaaa aattatcgtg
      661 ggcgaaaatg agtttttgtg gctcattagg tcaaaactca catattcgca ggacggcact
      721 gttgcaaata gtcggtggtg ataaacttat c
//
